LOCUS       DATIAZ010000001         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1841976, whole genome shotgun sequence.
ACCESSION   DATIAZ010000001 DATIAZ010000000
VERSION     DATIAZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841976"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..377
                     /locus_tag="VLF19_00005"
     CDS             63..377
                     /locus_tag="VLF19_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HSE91660.1"
                     /translation="MKGFGNLMKEAQRLQQQMAAMQEEIGQRKVDATAGGGMVTVQAN
                     GKQEIVSIKIDPEVINKDDAQMLEDLVLAACNEALRKSRELVQQELGKLTGGLKIPGL
                     GL"
     gene            387..989
                     /gene="recR"
                     /locus_tag="VLF19_00010"
     CDS             387..989
                     /gene="recR"
                     /locus_tag="VLF19_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HSE91661.1"
                     /translation="MAHYPEPVARLIEALQRLPGIGPKTAQRLTFFLLKRPAEEVREI
                     SEALVAVKDRIVYCQTCYNVTDENPCRICADPRRDGDLLCVVEEPNDLMAMERTGEYR
                     GRYHVLLGALSPLDGIGPEDLKVRELLARLDGGGATEVILATNPNVEGEATALYLAKL
                     LRPLGVRVTRIARGLPVGGDLEYADQVTLSKALEGRREIS"
     gene            1078..1671
                     /locus_tag="VLF19_00015"
     CDS             1078..1671
                     /locus_tag="VLF19_00015"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HSE91662.1"
                     /translation="MPRPGEASVPVWVVGHGWHVGLAVRIADVRVDVWPEPGRLGDVE
                     YVEVGWGDGAFYVAERATLALGLNAAVSSDSSVLHVAAFVPPVVDFFVASPIVELPLT
                     RAGFDDLSRYIADAYARDASGNPRRVGDGLYGTGGFYAARERYHVFNNSNQWAARALR
                     AGGVPVAASLALYGGTVISEAARFGVVRRATGSPARP"
     gene            2107..2182
                     /locus_tag="VLF19_00020"
     tRNA            2107..2182
                     /locus_tag="VLF19_00020"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2140..2142,aa:Asn,seq:gtt)
BASE COUNT          341 a          758 c          766 g          320 t
ORIGIN      
        1 ctggcggaat tcgagggcga ggtggtcgcg gtgcgcccgc gcccgccgga aggagaaggc
       61 cagtgaaggg gttcggcaac ctcatgaagg aagcgcagcg cctgcagcag cagatggcgg
      121 cgatgcagga ggagatcgga cagcggaagg tggacgcgac ggccggcggc ggcatggtga
      181 ccgtgcaggc caacggcaag caggagatcg tctcgatcaa gatcgacccc gaggtcatca
      241 acaaggacga cgcccagatg ctcgaagacc tcgtcctcgc cgcgtgcaac gaggcgctgc
      301 gcaagtcgcg cgagctggtc cagcaggagc tcggcaagct caccggcggc ctcaagatcc
      361 cggggctcgg cctctagggg ggcgccgtgg cgcactaccc cgagcccgtc gcgcgcctca
      421 tcgaggcgct ccagcggctg ccgggcatcg ggcccaagac ggcccagcgc ctgacattct
      481 tcctgctcaa gcgcccggcc gaggaagtgc gcgagatcag cgaggcgctc gtcgccgtca
      541 aggaccgcat cgtctactgc cagacctgct acaacgtcac cgacgagaat ccctgccgca
      601 tctgcgccga tccccgccgc gacggcgacc tcctctgcgt cgtggaggaa ccgaacgatc
      661 tcatggcgat ggagcgtacg ggcgagtacc gcggccgcta tcacgtgctg ctgggcgcgc
      721 tctcgcccct cgacggtatc ggccccgagg acctcaaggt ccgcgagctg ctggcgaggc
      781 tcgacggcgg cggcgccacc gaggtgatcc tcgccaccaa cccaaacgtg gagggcgagg
      841 cgaccgcgct ctaccttgcc aagctcctgc gcccgctcgg cgtgcgcgtg acgcggatcg
      901 cgcgggggct gcccgtgggc ggcgacctcg agtacgccga ccaggtgacg ctgtccaagg
      961 cgctggaggg ccggcgcgag atcagctagc cggcccggcg cacccggcgt cgccgccctg
     1021 atcgtcaccg gggcggcgct cctggtgctt gccggctgcg catccccgga tcagcgattg
     1081 ccgcgtcccg gcgaggcctc cgttcccgtg tgggtcgtcg gtcacgggtg gcacgtaggt
     1141 ctggccgtcc gcatcgccga tgtgcgggtc gacgtgtggc ccgagccggg ccgcctggga
     1201 gacgtcgagt acgtcgaggt cggctggggt gacggcgcgt tctacgtcgc ggagcgcgcc
     1261 acgctcgccc tcgggctcaa cgccgccgtc agctcggact cgagcgtgct ccacgtcgcg
     1321 gccttcgtgc cgccggtcgt ggacttcttc gtcgcgtcgc cgatcgtgga gctgcctctc
     1381 acgcgcgccg gcttcgacga cctctctcgg tacatcgccg acgcgtatgc ccgcgacgcc
     1441 agcggcaatc cgcggcgcgt cggcgatggc ctctacggca ccggcggctt ctacgccgcg
     1501 cgcgagcgct accacgtgtt caacaactcc aatcagtggg cggcgcgcgc cctgcgagcc
     1561 ggcggcgtgc cggtggcggc gtcgctcgcg ctctacggag ggacggtcat ctccgaagcg
     1621 gcgcgcttcg gcgttgtccg gcgtgcgacg ggcagtcccg cgcgtccctg aggcggccag
     1681 ggcattgtag aaaggtcccg gatggaggag gcaaccgcgc gcccgatctt cctgggtggc
     1741 aaccgtcatc acgtcacgcg tggtgcttcg gttgcccgaa cgattttcac gagccgacaa
     1801 gagccactcc gaccacggcc tggaacccgt ggcccccttg gacgcagccg cggagtgcat
     1861 ccctcgctct ccaagacgct catcgaacga tggcgaccgg gaactcgagg cggccctctg
     1921 ggccggaagc gaacatgcac cccgcgcgtt gaatcgtatg cgacgaggcg cgctaatcgg
     1981 gagatgtcga acgggtcgaa cggcgtccga agcaccagtc cccgagtcac tgtccgggac
     2041 ggatcttcgg tacggcatga tgccagccgc ggtacaatga cacgacgtcg cgtcagcgcc
     2101 ggtcactccc cggtagctca gtcggtagag cgcctgactg ttaatcagga tgtcgcaggt
     2161 tcgagacctg cccggggagc caact
//
LOCUS       DATIAZ010000002         8241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_789533, whole genome shotgun sequence.
ACCESSION   DATIAZ010000002 DATIAZ010000000
VERSION     DATIAZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789533"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..442
                     /locus_tag="VLF19_00025"
     CDS             164..442
                     /locus_tag="VLF19_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91663.1"
                     /translation="MTVRYAGARQAGLTEAKIAALDDLDSDLFSPRERAALTFAERMA
                     TDHHKVDDAVFAELRRHFSEAEIVELGVSTALFVGFGRFNAVLGIDPV"
     gene            487..1455
                     /locus_tag="VLF19_00030"
     CDS             487..1455
                     /locus_tag="VLF19_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSE91664.1"
                     /translation="MALDTTRLVKMLRTMVLIREFDELAIQLRVAGKIYGVVHPYVGQ
                     EAVAVGVCSNMTTSDRVTSTHRGHGHCIAKGADIRRMMAELFGRVDGYCKGRGGSMHI
                     ADFSVGMLGANGIVGGGLPIACGAALAAQLEGRGNVTVCFFGDGAAAEGEFHEALNIA
                     SVWQLPIVFVCENNQYAANNAVAVQHPRVDIAAHAGAYSMPGVVADGNDLLEVYAATG
                     EAVARARRGDGPSLLECKTYRWHFHAMRAVVPPETRPADEIASWKARDPIARFEHELL
                     ERGALSAFEIRAMRERVKAELAEAVAFAEASPFPDSKDLLVDMFAE"
     gene            1524..2444
                     /locus_tag="VLF19_00035"
     CDS             1524..2444
                     /locus_tag="VLF19_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSE91665.1"
                     /translation="MRRDPRVILMATEFTGDPVNEFGPTRVRLTPVSEAVLTGMGLGA
                     AGCGFRPIVNWRMVTFSFVAMDQIVNQASKIRYMFGGQADFPVTYRCTTGGGIGLAAQ
                     HSQSPYSMWMHLAGLKIVLPSTPADAKGLLKSAIRDNNPVVCFECSRLATATGPVPDG
                     DHLVPIGVAEVKRVGTDVTIVGLAYYVSQALAVAEELASEDISVEVVDPRTLVPMDIG
                     TLHVSVRKTGRVVIVDEAPATCSAASEIAALLVEDPETFRALRAPVHRVCAVPVPVPY
                     SPTLEHAALPTRDDIRTAVYEVLKDSSWRL"
     gene            2610..3464
                     /locus_tag="VLF19_00040"
     CDS             2610..3464
                     /locus_tag="VLF19_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HSE91666.1"
                     /translation="MERSFPIVRHPVAQKRTPVLVSVPHYGTQPLPHITRDDYCEPWF
                     ETFAYGFADTFAGDLYSDLHEHGATVLATPFSRMFVDVNRRRDDFEHHDGEVRSRRGV
                     VRTHTMRDVPIFARPLGLADLEVRLQTLYDPYYSALEGLLTQLRDAYGCAMLLDGHTG
                     SPRRMKDHQVIIGTRHETTCAPQLAATVATIFTRHGFEVHENVSGYAGGNIVTTFGQP
                     QARRIHALQLEINASLLMTTSREEFIGHVSRGGVPEKAEENIARIRRCLREVLAALPS
                     VLVTMHDP"
     gene            3613..4365
                     /locus_tag="VLF19_00045"
     CDS             3613..4365
                     /locus_tag="VLF19_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE91667.1"
                     /translation="MDLELGGHTAVVTGCSVGIGREIARVLAAESVPALVVARRGELL
                     KTLQDEIEAAGWVRPHALAIDLADRAAPARVRDEAQARLGRVDILVNNAGGSRPTAVD
                     APDGVWDESFALNFTSVRKLTQALLPGMIERRWGRILNVTGTSEPAGTNAAGPAKAAV
                     HAWAKGLSRDVGRHGITVNCLAPGRIHSEQIDQRLHPTPESQEQFARQIPLGYFGDPC
                     DMAYLVAFLCSPKARYITGQRIHVDGGLHRAV"
     gene            4489..5460
                     /locus_tag="VLF19_00050"
     CDS             4489..5460
                     /locus_tag="VLF19_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HSE91668.1"
                     /translation="MKAIVFTQTGGPEVLALADVPRPDVRPGMVLIKVHAVGVNFADT
                     RFRQGTYVVKPKLPDTPGMEASGVVDAVGDGVTDLRPGARVTAFTVKSYAEYCQAPAP
                     MVIPLPGFVSFVEGAAFPIQVLTAYHMLHTADSTGPGRTVLVHSAAGGVGLAAVQLAK
                     VAGARVFGTVSSDAKAPLVREHGADAVINYVTEKFADEVLRLTDGRGADLILDAVGKP
                     TFEEGLRCLAVFGHLILYGRAGGPTDPLNVATLSPKSQKVSGFMVPTVTRNFPDKTRE
                     SADRCFALMREGRLKLHVGKTFPLAQAAEAHRYLESRQSTGKLILLP"
     gene            complement(5473..6642)
                     /locus_tag="VLF19_00055"
     CDS             complement(5473..6642)
                     /locus_tag="VLF19_00055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSE91669.1"
                     /translation="MIVSRAVTHNLKEASWIRRMFEEGARLKQERGADKVFDFTLGNP
                     EIEPPAAVLAAARRVLDSDTPHLHAYMPNAGHPSVREAVAGRLRAATGLPYTANHVLM
                     TVGAGAALNTVLKALLDPGDEVVTVAPFFAEYVFYAENHGGRLVVVPPRPDLTPDIAR
                     LEAAITPRTRAILVNSPNNPSGVIYPASTFVEIEALLARAARPIVLISDEPYRALVFD
                     DVVPPEVPPLVTRSIVATSWSKSLAIPGERIGYLAFSPRMPEAAELFEACTFTSRVLG
                     FVNAPALWQWVVAEVGDLVIDVAPYREKRDLMYEGLTRIGYPCVKPRGAFYVFPRTPI
                     DDDVAFVRLLAEEGVLTVPGSGFGMSGHIRISLTVERDTVVRALPGFERAFRKAH"
     gene            6675..7448
                     /locus_tag="VLF19_00060"
     CDS             6675..7448
                     /locus_tag="VLF19_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE91670.1"
                     /translation="MSPRMAVHRRGSGPDVVLFHGGMGSWKHWIRNVEPLATRFTVHA
                     LDHPSYGASATVPRETAGAAYLDLVHELFVEMFPGDAPLRLAGFSFGGAIAANLARRL
                     APRVTHLCLVSPGGFPPRRFGDRPTRSYREAGNDEKLFRDICRHNLLVNMLGEEDSVT
                     EDTVDIQAECVRRTRFNSRKVSGGGTLLGDLRRLTCRIRLLWGERDDSPFRPADLLIA
                     EIREAVGTLDVHRIPRAGHWSAYENAPEVNRLMLEFFSS"
     gene            7542..8075
                     /locus_tag="VLF19_00065"
     CDS             7542..8075
                     /locus_tag="VLF19_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSE91671.1"
                     /translation="MITDIASYLRFFDSVRRRTERDVAALPPEAATWRPPGPGGEAGW
                     SIGDIVGHIGGSRLYFARAYRGEGWITTPPEVDRADQRTWLPWLQSSAERFVAQLRDT
                     PDAWLTRRIQMIDTPGSLSGWRMLMMMLEHEVHHRSQIDTYAGLQGWPVPDIFERSAE
                     TIAALQPAQRARHQTRG"
BASE COUNT         1245 a         2926 c         2860 g         1210 t
ORIGIN      
        1 cagcgggagc tcggcgagca cgcggaccct cgcccaccac ccaccagcgg tcaaggcgct
       61 cgccgccttt cgccgtgcgc tcgccaagga gagcgtgctc gacccgaccc tgaccgagct
      121 ggtgcggctc aaggtggcca accgcaacgc ctgtcgctac tgaatgacgg tccgctatgc
      181 cggggcccgg caagccggac tcaccgaagc caagatcgcg gcgctcgacg acctcgactc
      241 ggatctcttc agccctcgcg agcgcgccgc cctcaccttc gccgagcgga tggcgaccga
      301 ccatcacaag gtcgacgatg ccgtgttcgc cgagctcagg cgccacttct ccgaggcgga
      361 gatcgtcgag ctgggcgtgt ccaccgcact cttcgtcggg ttcggccgct tcaacgccgt
      421 tctcggcatc gatcccgtgt agcggcaccg ccccagcctg agcggctgat cgagggaggg
      481 cgcgcgatgg cgctcgacac gacgcgactg gtcaagatgc tgcgcacgat ggtcctgatc
      541 cgggagttcg acgagctggc catccagctg cgggtcgccg gcaagatcta cggggtggtg
      601 catccctacg tgggccagga ggcggtcgcg gtcggggtgt gctcgaacat gacgacgagc
      661 gatcgcgtga ccagcactca ccgcggccac gggcactgca tcgccaaggg cgccgacatc
      721 aggcgcatga tggccgagct gttcggccgc gtggacggct attgcaaggg caggggcggc
      781 tccatgcaca tcgccgactt ctcggtgggg atgctgggcg cgaatggcat cgtcggcggc
      841 gggctgccga tcgcctgcgg ggcggcgctg gccgcgcagc tcgagggccg gggcaatgtg
      901 accgtgtgct tcttcggcga cggcgcggcc gccgagggcg agttccacga ggcgctgaac
      961 atcgcctcgg tgtggcagct gcccatcgtc tttgtctgcg agaacaacca gtacgcggcg
     1021 aacaacgccg tcgccgtgca gcatccgcgg gtcgacatcg ccgcccacgc cggcgcctac
     1081 tccatgcccg gcgtcgtcgc cgatggcaac gacctgctcg aggtgtacgc ggccaccggc
     1141 gaggccgtgg cccgcgcgcg ccggggcgac ggaccgagtc tgctcgagtg caagacgtat
     1201 cgctggcact tccacgccat gcgggccgtc gttcccccgg agacccgccc ggccgacgag
     1261 atcgcatcct ggaaggctcg cgatcccatc gcgcgcttcg agcacgagct tctcgagcgg
     1321 ggcgcgctct ccgctttcga gatccgcgcc atgcgcgagc gcgtgaaggc cgagctcgcc
     1381 gaggccgtgg cctttgccga ggccagcccg ttccccgatt cgaaagacct gctcgtcgac
     1441 atgttcgccg agtgagcgcc gcggaacgcg ccctgcgcga gatcagcttc cagaaggccc
     1501 tcgaggaggc cgtggccgag gagatgcggc gcgatccccg tgtcatcctc atggccacgg
     1561 aattcacggg ggaccccgtc aacgagttcg gcccgacgcg cgtacgcctc acgcccgtct
     1621 ccgaggcggt gctgacgggc atgggtctgg gggcggcggg ctgcggcttc cgtcccatcg
     1681 tgaactggcg gatggtgacg ttctcgttcg tggcgatgga tcagatcgtc aaccaggcga
     1741 gcaagatccg gtacatgttc ggcgggcagg ccgatttccc ggtcacctat cgctgcacca
     1801 ccggaggagg catcggcctc gccgcccagc actcgcagag cccgtactcg atgtggatgc
     1861 acctcgcggg cctgaagatc gtcctgccct ccacgccggc cgacgccaag ggcctgctca
     1921 agagcgccat ccgcgacaac aatcccgtcg tctgcttcga gtgcagccgg ctggccaccg
     1981 cgaccggccc ggtgcccgac ggggaccatc tcgttccgat cggggtcgcc gaggtcaagc
     2041 gcgtgggcac ggacgtgacg atcgtgggcc tcgcctacta cgtgtcccag gcgctggcgg
     2101 tggcggagga gctggcgagc gaggacattt ccgtcgaggt ggtcgacccg cgaaccctgg
     2161 tccccatgga catcggcacg ctgcacgtct cggtccgcaa gaccggccgc gtcgtcatcg
     2221 tggacgaagc gcccgccaca tgctcggcgg cgtcggagat cgccgcgctg ctggtcgagg
     2281 atcccgagac gtttcgcgcc ttgagggccc ccgtccaccg cgtctgtgcc gtccccgtgc
     2341 ccgtgcccta cagccccacg ctggagcacg ccgcgctgcc gacccgcgat gacatcagga
     2401 cggcggtcta cgaggtgctc aaggactcct cgtggcggct gtagcgctcg agcgcccgcc
     2461 ccgtcgatgg cggacgcgag agagccgcca cgcgcgcggg cgagcgcgcg ccgtgcttcc
     2521 ggggtggccg tccgggcgca ccttcaccct cgcacggatc ccgcagcgca ccgtcgtccg
     2581 cggcacggtc ccgttctgag cggagcccga tggagagatc gtttccgatc gtcagacacc
     2641 ccgtggcgca gaagcgcacg cccgtgctgg tgagcgtgcc ccactacggc acgcagcccc
     2701 tgccccacat cacccgcgac gactactgcg aaccctggtt cgagaccttc gcgtacggct
     2761 tcgccgatac cttcgcgggc gatctctaca gcgacctgca cgagcacggg gccaccgtcc
     2821 tggccacgcc gttctcgcgc atgttcgtcg acgtcaatcg ccggcgcgac gacttcgagc
     2881 atcacgacgg cgaggtgcgg tcccggcgcg gggtggtgcg cacccacacc atgcgcgatg
     2941 tgcccatctt cgcgcgcccg ctcgggctcg ccgacctcga agtgcgactg cagacgctct
     3001 acgaccccta ctactcggcc ctcgagggcc tgctcaccca gctccgggac gcctatgggt
     3061 gtgcgatgct cctcgatggc cacaccggca gcccgcgccg catgaaggac catcaggtga
     3121 tcatcggcac ccgccacgag accacgtgtg ccccacagct ggcggcgacg gtggccacga
     3181 tcttcacccg ccacggcttc gaggtgcacg agaacgtcag cggctatgct ggcggcaata
     3241 tcgtgaccac gttcggtcaa ccacaggccc ggcgcatcca cgcgctccaa ctggagatca
     3301 acgcgtcgct gctgatgacg accagccgcg aggagttcat cggccacgtg tcacgaggag
     3361 gcgtccccga gaaggcggaa gagaacatcg cccgtatccg tcggtgcctc cgggaggtgc
     3421 tggcggccct gccctccgtc ctcgtgacca tgcacgatcc gtagcgccgc gctcgccggc
     3481 ccctcccgga cgcgaccttg cctcggcgcc atccagaggg cacaatcgcg cggcgccgcc
     3541 gattcgcggt cagtgggcac ggtcgtgaca acgcctggcc gcgacgccga cgccagcccc
     3601 ggggaggagt cgatggatct cgagcttggc ggccatacgg ccgtggtgac cggttgcagc
     3661 gtgggaatcg ggcgggagat cgcccgcgtc ctcgccgccg agagcgtgcc cgccctcgtg
     3721 gtcgcgcggc gtggcgagct cttgaagacc ctccaggacg agatcgaagc cgcggggtgg
     3781 gtgcgccctc acgccctggc gatcgatctc gccgaccgcg cggcgcccgc gcgggtccgt
     3841 gacgaggccc aggcccggct cggccgcgtg gatatcctcg tgaacaacgc cggcgggtcc
     3901 cggcccaccg cggtggacgc ccccgacggc gtgtgggacg agtcgttcgc cctcaacttc
     3961 acctcggtcc gcaagctcac ccaggcgctc ctgcccggca tgatcgagcg gcgctggggc
     4021 cggatcctca acgtcaccgg gacgtcggag ccggccggca ccaacgcggc ggggccggcc
     4081 aaggccgcgg tgcacgcgtg ggcgaaggga ctctcgcgcg acgtgggccg ccacggcatc
     4141 accgtgaact gcctcgcccc cgggcggatc cacagcgagc agatcgacca gcgcctgcac
     4201 cccacgcccg agagccagga gcagttcgcc cgccagattc cgctgggcta cttcggcgac
     4261 ccctgcgaca tggcctacct ggtggcgttt ctctgttcgc ccaaggcgcg ctacatcacg
     4321 ggccagcgta tccacgtgga cggcggcctc caccgcgccg tgtgacggct gggggccccg
     4381 acatggcccc cttgcccccg gcgttcggag cgtctcggcg aagtcgatgc gctccactat
     4441 actgcggcgg gctaccgcgg caggctccgg gacagaaatg agggagaaat gaaggcgatc
     4501 gtcttcacgc agaccggtgg tcccgaggtg ctggcgctcg ccgacgtgcc caggcccgac
     4561 gtgcggcccg ggatggtgct catcaaggtc cacgcggtcg gggtgaattt cgcggacacg
     4621 cgctttcgcc agggaacgta cgtcgtgaag cccaagcttc ccgacacgcc gggcatggag
     4681 gcgtcggggg tcgtcgacgc cgtcggcgac ggcgtgaccg atctccgccc gggagcgcgg
     4741 gtgaccgcct tcacggtcaa gtcgtacgcg gagtactgtc aggcgccggc gcccatggtc
     4801 attccgctgc ccggcttcgt gagcttcgtg gagggcgccg cgttcccgat ccaggtgctg
     4861 accgcctatc acatgctgca cacggcggac tcgaccgggc cgggtcgaac ggtgctcgtc
     4921 cactcggccg cgggcggggt cggcctcgcc gcggtgcagc tggcgaaggt ggccggtgcc
     4981 cgcgtgttcg gcacggtatc cagtgacgcc aaggcgccgc tcgtgcggga gcacggcgcc
     5041 gacgcggtca tcaactacgt caccgagaaa ttcgccgacg aggtgctgcg cttgaccgat
     5101 ggccgcgggg ccgacctcat cctcgacgcc gtggggaagc cgaccttcga ggagggactg
     5161 cggtgcctcg cggtgttcgg gcacctcata ctctacgggc gcgccggggg gcccacggat
     5221 ccgctcaacg tcgccacgct gtcgccgaag tctcagaagg tcagtggctt catggtgccg
     5281 acggtcaccc ggaactttcc ggacaagacg cgcgagagcg cggaccgctg cttcgccctc
     5341 atgcgcgagg gccggctcaa gctacacgta ggaaagacgt tcccgctcgc ccaggcggcc
     5401 gaggcgcacc gctacctcga atcgcggcag tcgaccggaa agctgatctt gctcccgtga
     5461 ccgaggagcc gttcagtgag ccttgcgaaa ggcgcgctcg aagccaggga gcgcgcgcac
     5521 cacggtgtca cgctccaccg tgagcgagat ccggatgtgg ccggacatgc cgaagccgga
     5581 gccgggcacc gtgaggacgc cctcctcggc cagcaagcgg acgaaggcca cgtcgtcgtc
     5641 gatgggcgtg cgcgggaaca cgtagaaggc gccccggggc ttcacgcacg ggtagccgat
     5701 gcgggtcagt ccctcgtaca tcagatcgcg cttctcgcga tagggcgcca cgtcgatcac
     5761 cagatccccc acctccgcca ccacccactg ccagagcgcc ggcgcgttca cgaagccgag
     5821 cacccggctg gtgaacgtgc aggcctcgaa gagctcggcg gcctccggca tgcgcgggga
     5881 gaaggccaga tagccgattc gctcccccgg aatggccaga gacttggacc aggacgtcgc
     5941 gacgatcgag cgggtcacca gaggcggcac ctcgggcggc accacgtcgt cgaagacgag
     6001 ggcgcggtag ggctcgtcgc tgatgagcac gatgggccgg gcggcgcggg cgagcagggc
     6061 ctcgatctcg acgaacgtcg acgcggggta gatgacgccg ctcgggttgt tcggcgaatt
     6121 gaccaatatt gcccgcgtcc gcggggtgat ggcggcctcg agcctcgcga tgtccggcgt
     6181 gaggtcgggt ctcggcggca ccacgaccag ccgcccgcca tggttctcgg cgtagaacac
     6241 gtactcggcg aagaaggggg ccaccgtgac gacctcgtcg ccagggtcga gcagggcctt
     6301 cagcacggtg ttgagcgcgg cgccggcacc caccgtcatg aggacgtgat tggccgtgta
     6361 cggcagcccg gtggccgcgc ggagccgccc ggccaccgcc tcgcggacgg acggatggcc
     6421 ggcgttgggc atgtaggcgt gcaggtgggg cgtgtcgctg tcgagcacgc gccgggccgc
     6481 ggccagcacc gcggccggcg gctcgatctc ggggttcccc agcgtgaagt cgaagacctt
     6541 gtcggcgccg cgctcctgct tgaggcgagc cccctcctcg aacatgcgac ggatccacga
     6601 ggcctctttc aagttatgcg tgacggccct ggagacgatc atcgtcggag tatagtcgac
     6661 gcggaggagg tcgcatgagc ccccggatgg cggttcaccg gcgcggcagc ggaccggacg
     6721 tcgtgctctt tcacgggggc atgggttcgt ggaagcactg gatccggaac gtcgagccgc
     6781 tcgccacgcg cttcaccgtg cacgccctcg atcatccgtc gtacggcgcc tcggcgaccg
     6841 tcccgcgcga gacggccggc gcggcgtacc tggacctcgt gcacgagctg ttcgtcgaga
     6901 tgttcccggg cgatgccccg ctgcggctgg ccgggttctc gttcgggggg gccatcgccg
     6961 cgaacctggc ccgacggctc gccccgcggg tgacccacct gtgcctggtc tctccgggcg
     7021 gcttcccgcc acgtcgcttc ggcgaccggc cgacccggag ctacagggag gccgggaacg
     7081 acgagaagct cttccgcgac atctgccgcc acaacctgct cgtcaacatg ctcggcgagg
     7141 aggacagcgt caccgaggac acggtggaca tccaggccga gtgtgtgcgc cggacgcgct
     7201 tcaacagccg caaggtgagc ggcggcggca cgctcctggg cgacctccgc cggctcacct
     7261 gccgcatccg gctgctctgg ggcgagcgcg acgattcacc gttccggccg gccgatctgc
     7321 tgatcgccga gattcgcgag gccgtcggca cactcgacgt gcaccggatt ccccgggccg
     7381 gccactggtc ggcgtacgag aacgctcccg aggtcaatcg cctgatgctg gagttcttct
     7441 cgagctgacg acgcgcacac gatcaggctc ggcgcgccga gtcctgctag gatgatcgcc
     7501 gaccggcgac ggcgcgcttc cggcgcgagg aggatggccc gatgatcacc gacatcgcgt
     7561 cctatctccg cttcttcgac agcgtgcggc ggcggaccga gcgtgacgtg gcggcgctcc
     7621 cgccggaggc ggcgacgtgg cggccgcccg gccccggtgg cgaggccggg tggagcatcg
     7681 gcgacatcgt gggtcacatc ggcggctcgc gtctctactt tgcccgcgcc tatcgcggcg
     7741 aggggtggat cacgacgccg cccgaggtcg atcgcgccga ccagcggaca tggctcccgt
     7801 ggctccagtc gtccgccgag cgcttcgtcg cccagctccg ggacacaccg gacgcatggc
     7861 tcacgcgccg gatccaaatg atcgacacgc ccgggtcgct ctccggctgg cggatgctca
     7921 tgatgatgct ggagcacgag gtccaccacc gctcgcagat cgacacctac gcgggcctcc
     7981 agggctggcc ggtgcccgac atcttcgagc gctccgccga gaccatcgcc gccctgcaac
     8041 cggcccagcg cgctcgccat cagacgcggg gctgaccggt cgaagggcga cgagtccggc
     8101 ccgggtgggc gcggcctcag ctccagtccc gcgaggccgc tggcgtcatc cttgccgaga
     8161 cctagcggtc gaggacgagc gggacgtccc ggtcgttgcg gccgcgcgcg aagatctcca
     8221 gggcgtccgg aagctcgtag t
//
LOCUS       DATIAZ010000003         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1842071, whole genome shotgun sequence.
ACCESSION   DATIAZ010000003 DATIAZ010000000
VERSION     DATIAZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842071"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VLF19_00070"
     CDS             <1..474
                     /locus_tag="VLF19_00070"
                     /inference="COORDINATES: protein motif:HMM:NF039584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HSE91672.1"
                     /translation="IAHALGIRTNATMLYGHVETMVERVDHLLRLREQQDASGGFLTF
                     VPLAYQVGTTRLRPRQTPPTDSLRTIAAARLLLDNFPHVEAYWVVLGEDTTSVALHFG
                     ADDVNGTLEDERIQHMAGAQTPAGVAREQLLRMIRDAGKTAVERDALYNVVQAYS"
     gene            501..1547
                     /gene="mqnC"
                     /locus_tag="VLF19_00075"
     CDS             501..1547
                     /gene="mqnC"
                     /locus_tag="VLF19_00075"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HSE91673.1"
                     /translation="MSGQRLDREDGRWLLAEAPLLELGALAQEVRFARIPERRVTFVI
                     DSNPNYTNVCVTDCQFCAFYRKPGDPEAWTLTVDEVLAKVGAAASRGATTVLLQGGHN
                     PALPLDYYLTLVRETRRRFPQVTPHFFTASEIRTIAQVAALGVGDVLDRLWDAGQRTI
                     PGGGAEVLSERVRRRIEPKKGGPEAWLDVHHEAHRRGFRSTATMMYGHVETVEDLLDH
                     LDAIRRLQDAHSGFTAFVPWSFKPGHTLLEKWITHYQGPNTYLRMLAAARLYLDNFPH
                     VQASWFSEGKRTGQIALQFGADDWGGTLFEENVHKAADYVNTIGVEELTTLIREAGFT
                     PVQRTTTYEIIRVW"
     gene            complement(1561..2391)
                     /locus_tag="VLF19_00080"
     CDS             complement(1561..2391)
                     /locus_tag="VLF19_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HSE91674.1"
                     /translation="MATIRIGHSPDPDDAFMFYALTAGKVRIPGAEVEHVLEDIESLN
                     RRARTAELEVTAVSAATYVLVADRYRLMDPGASMGKGYGPILVAREPLAAEQITQRVV
                     AIPGSHTTASLLLRLYLPEEPPLIEVTFDKIPRAVLEGQADVGLLIHEGQITHRSMGL
                     VKVLDLGELWQRDTALPLPLGVNVMRRDLGEDTHRAISSALRESIAWAHTHVDEALEY
                     AMRYGRGIDKETCRRFVLMYVNDYTVTLGNDGRTALERLFTMAHRRGLIPAVPPLDPV
                     "
     gene            complement(2402..2983)
                     /locus_tag="VLF19_00085"
     CDS             complement(2402..2983)
                     /locus_tag="VLF19_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSE91675.1"
                     /translation="MALLRGNLHAHTTFSDGVLAPHRLVEEYERLGYDFLAITDHDDL
                     VAESYWRALERLAPRLLLFHGIELSWQAFPQHVGKVVGDRETLFVLNHPARYKLSVAD
                     TLWRVGVIRADGVPLDAIEVTDTGLYRPLYHSASIPLVKVATDDAHKPVHFGRAWVEV
                     DAARDRDAIIRAIRAGDFALGFRQPELPAAPSA"
     gene            complement(2984..3412)
                     /locus_tag="VLF19_00090"
     CDS             complement(2984..3412)
                     /locus_tag="VLF19_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91676.1"
                     /translation="MRVGAAAFVVSMMIGGVAPAVAQQVDITAPVPRRTAPVPDRERE
                     AVVLPPGDHRDVTRPSDADYYPRGPRVRHDPAYIVPLSSRIAEGTGRAGIAGWTAPNT
                     PASGSEASGFREVTGWFALGIAVEWGGPPPRRAPARAPGR"
BASE COUNT          544 a         1325 c         1276 g          522 t
ORIGIN      
        1 atcgcccacg ccctcggcat ccgcacgaac gccaccatgc tctacggtca cgtcgagacg
       61 atggtcgagc gcgtggatca tctgctgcgc ctgcgcgagc agcaggacgc ctccggcggc
      121 ttcctgacgt tcgtccccct cgcctaccag gtcggcacga cgaggctgcg cccgcggcag
      181 accccgccga ccgacagcct gcgcaccatc gccgccgcgc ggctgctcct cgacaacttc
      241 cctcacgtcg aggcgtactg ggtggtcctc ggcgaggaca cgacgtcggt ggccctgcac
      301 ttcggcgccg acgatgtcaa tggcacgctg gaggacgagc gcatccagca catggccggc
      361 gcgcagacgc cggccggcgt ggcgcgcgag cagctgctgc ggatgatccg cgacgccggc
      421 aagaccgccg tcgagcgcga cgcgctctac aacgtggttc aggcctacag ttagtgctgg
      481 accgcatcgc cgacaaggcg ctgagcggcc agcgtttgga ccgcgaagac ggccggtggc
      541 tcctcgccga ggcgccgctg ctcgagctgg gcgcgctggc gcaggaggtt cgcttcgcgc
      601 ggatccccga gcgccgcgtc accttcgtca tcgactccaa ccccaactac acgaacgtct
      661 gcgtcaccga ctgccagttc tgcgcgttct atcgcaagcc cggcgacccc gaggcctgga
      721 cgctgacggt ggacgaggtc ctggccaagg tcggcgccgc ggcctcccgc ggcgcgacga
      781 cggtgctgtt gcagggcggc cacaatcccg ccctgccgct cgactactat ctgaccctcg
      841 tccgcgagac gcggcggcgg ttcccccagg tgaccccgca cttcttcacg gcctccgaga
      901 tccgcacgat cgcccaggtc gccgcgctcg gcgtcggcga cgtgctcgac cgcctgtggg
      961 acgcgggcca gcggaccatc cccggcggcg gcgccgaggt gctctccgag cgcgtgcgcc
     1021 gccggatcga gcccaagaaa ggcggcccgg aggcctggct cgacgtccac cacgaggcgc
     1081 accggcgtgg cttcaggtcg acggcgacga tgatgtacgg ccacgtggag acggtggagg
     1141 atctcctcga ccatctcgac gcgatccgcc ggcttcaaga cgcgcacagc ggcttcaccg
     1201 cgttcgtgcc ctggtcgttc aagcccggcc acacgctgct cgagaagtgg atcacgcact
     1261 accagggccc gaacacatac ctccgcatgc tcgccgcggc acggctgtac ctcgacaact
     1321 tcccccacgt ccaggcctcg tggttctcgg agggcaagcg caccggccag atcgcgctgc
     1381 agttcggcgc cgatgactgg ggcggcacgc tcttcgagga gaacgtgcac aaggccgcgg
     1441 actacgtgaa cacgatcggc gtggaggagc tgacgacgct gatccgcgag gcgggcttca
     1501 cgcccgtcca gcgcaccacc acctacgaga tcatcagggt ctggtagagg gccgccgggc
     1561 tcagaccggg tcgagaggcg gcaccgccgg gatgagcccg cgccggtgcg ccatcgtgaa
     1621 caggcgttcc agcgccgtcc ggccgtcgtt gccgagcgtc accgtgtagt cgttcacgta
     1681 catgagcacg aagcggcggc acgtctcctt gtcgatcccc cggccgtagc gcatcgcgta
     1741 ctcgagagcc tcgtccacgt gggtgtgggc ccacgcgatg gattcccgta gcgccgacga
     1801 gatcgcgcga tgcgtgtcct cgccgaggtc gcgccgcatg acgttgacgc cgagcggcag
     1861 cggcagcgcc gtgtcgcgct gccacagctc tccgaggtcc agcaccttca ccagtcccat
     1921 cgaccgatgg gtgatctgcc cctcgtggat cagcaggccg acgtccgcct ggccctcgag
     1981 gacggcgcga gggatcttgt cgaacgtcac ctcgatgagc gggggctcct cgggcaggta
     2041 caggcgcagc aggagcgatg cggtcgtgtg gctgcccgga atggccacga cgcgctgggt
     2101 gatctgctcc gcggcgagcg gctccctcgc gaccaggatc ggcccgtagc ccttgcccat
     2161 cgacgccccg ggatccatga gccggtaccg gtcggcgacg agcacgtagg tcgcggccga
     2221 caccgcggtc acctccagct cggcggtgcg cgcacgccgg ttcagcgact cgatgtcctc
     2281 gagcacgtgc tcgacctcgg cgccggggat ccggaccttg cccgcggtga gggcgtagaa
     2341 catgaacgcg tcgtcgggat ccgggctgtg gccgatgcgt atcgtcgcca tcgaggctcc
     2401 gctacgccga aggcgcggcc ggcagctcgg gctggcggaa gccgagcgcg aagtcacccg
     2461 cccggatggc ccggatgatc gcgtcgcggt cgcgcgccgc gtccacttcg acccatgcgc
     2521 gcccgaagtg cacgggcttg tgcgcgtcgt ccgtcgcgac cttgaccagc gggatcgacg
     2581 ccgaatggta gagcggccgg tagaggcccg tgtccgtcac ctcgatggcg tcgagtggca
     2641 ccccgtcggc ccggatcact ccgacgcgcc agagcgtgtc cgccacgctg agcttgtagc
     2701 gcgccgggtg gttcaggacg aagagcgtct cgcgatcgcc cacgaccttg cccacgtgct
     2761 gcgggaacgc ctgccaggag agctcgatgc cgtgaaagag cagcagccgc ggcgccaggc
     2821 gctcgagggc tcgccagtac gactccgcga cgaggtcgtc gtggtcggtg atggccagga
     2881 agtcgtagcc gaggcgctcg tattcctcca cgagccggtg cggggcgagg acgccgtcgg
     2941 agaacgtcgt gtgggcgtgg aggttaccgc gaaggagagc cactcagcga ccgggcgcgc
     3001 gcgctggcgc ccggcgcggc ggcgggccgc cccactcgac cgcgatgccc agtgcgaacc
     3061 agcccgtgac ctcacggaac ccgctcgcct ccgagccgct ggccggggtg ttcggcgccg
     3121 tccagccggc gattcccgcg cggccggtgc cctccgcgat cctcgacgac aggggcacga
     3181 tgtaggccgg gtcgtggcgc acccggggtc cgcgcggata gtagtcggcg tcgctcgggc
     3241 gcgtgacgtc acggtggtcg ccgggcggga gcaccacggc ctcgcgctcg cggtcgggca
     3301 ccggcgcagt acgccggggc accggcgcgg tgatgtccac ttgctgggcg accgccggcg
     3361 ccaccccacc gatcatcatc gacaccacga acgctgccgc tcccacgcgc atgcttccat
     3421 cttactccag aaccagcgcc tcgccacttc gagggcggac gggtctcgac gaacgtcttt
     3481 cgatcctctc ccagcccgcc cgggccggtt gatgccggtg ctcgaacggc caccgagcca
     3541 cggcgctatc cgcgcggcag gacgaggggc gggcgacggc tcagccgggg cgggccgagc
     3601 gtgggggact gtgggaggcc cgatccgtgt ctcccgcgcg atcgaggacg gcgtcggcat
     3661 cgatctc
//
LOCUS       DATIAZ010000004         2728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1053255, whole genome shotgun sequence.
ACCESSION   DATIAZ010000004 DATIAZ010000000
VERSION     DATIAZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053255"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..294
                     /gene="rbfA"
                     /locus_tag="VLF19_00095"
     CDS             1..294
                     /gene="rbfA"
                     /locus_tag="VLF19_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSE91677.1"
                     /translation="MLLQRELKDPRLGFVTVTGVETTKDLRSAKVFVSVLGDEQQWRA
                     SMAALLSARGFIRNWLRQHLNLRVTPELDFRADHSMEHAARIQELLKQLSSGC"
     gene            306..1268
                     /locus_tag="VLF19_00100"
     CDS             306..1268
                     /locus_tag="VLF19_00100"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HSE91678.1"
                     /translation="MTPTDAVLAAFRRPPQRALMLGHVHPDADVIGTLLALGLALEAR
                     GWTVAYGGPHPVPDLLAFLPGVERYRRLDRVEAPLDIAVLTDCPNPARTEGLLEQARA
                     TGATVVNIDHHHDNRRYGDVNWVDPAAAATGEMVHDLLMALGAPLTPAIATNLFTAVH
                     MDTGSFRYSNVTPDTFRIAGALVAAGADPAFVSGALYERRPREALRWLGEALSRVEVS
                     GDGRVGWLALPAGLVPEAFVEAEDLVNFPRSVASVRVACLLRERDGAVKVSLRGKGDV
                     DVSRVAARFGGGGHPNAAGCTVKTSLDAATRQVLEAVGEALGER"
     gene            1265..2185
                     /gene="truB"
                     /locus_tag="VLF19_00105"
     CDS             1265..2185
                     /gene="truB"
                     /locus_tag="VLF19_00105"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSE91679.1"
                     /translation="MTASHRSGVLVVDKAAGPTSFDVVGLVRRLFRHRRVGHAGTLDP
                     GATGVLPILLGEATKLMPYLQEMDKDYVTTVRLGVRTDTQDLTGRVLETAPVPPLAPI
                     DVERAAVSFVGAIRQVPPMYSAVHHGGRRLYELAREGFEVAREGREVVVREITVEEVA
                     LPLVRLRIVCGKGTYVRTLAADLGEALGCGGAVEALRRTRVGPFTLDDAVPFSMLTAM
                     SADALGARVRPPSAALAGWPTVRLAESVAARFRHGQAVEVVTAPVSGARVAVLSDDDT
                     LLGVGEVAAGRVKPARILHADHPGPRVLPV"
     gene            2148..>2728
                     /locus_tag="VLF19_00110"
     CDS             2148..>2728
                     /locus_tag="VLF19_00110"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91680.1"
                     /translation="MRTIRGLESYPSEARPAVVALGVFDGVHLAHRAILAKAVGSALD
                     AGLAPVVCTFDPHPMEVLQPARAPTPLSSLDERLDLIAATGVQATLVLTFTPELAAME
                     PEPFVTDVLIERLRAREVVVGFNHTFGRGARGDARLLRALGDRQGFATHVVEPLVVDG
                     VPVSSSEIRGALRGGAVERAARYLGRPYAVAGEV"
BASE COUNT          333 a          972 c         1003 g          420 t
ORIGIN      
        1 ctgctgctgc agcgcgagct caaggacccg cggctcggat tcgtcaccgt caccggggtc
       61 gagacgacca aggacctccg ctcggcgaag gtgttcgtct ccgtcctggg cgacgagcag
      121 cagtggcggg cctcgatggc cgcgctcctg tcggcgcggg gcttcatccg caactggctg
      181 cgccagcacc tgaacctccg tgtcacgccc gagctcgact tccgagccga ccactcgatg
      241 gagcacgctg cgcgcatcca ggagcttctc aagcagctct cctcgggctg ctgacgggtg
      301 gcgcggtgac cccgaccgac gcggtcctgg ccgcctttcg ccgccctccg cagcgcgcgc
      361 tgatgctcgg ccacgtgcac ccggatgccg acgtcatcgg cacgctgctc gccctcggcc
      421 tggcgctgga ggcgcgcggc tggacggtgg cctacggagg gccccacccg gtgcccgacc
      481 tgctcgcgtt cctgcccggc gtggagcgct accgtcggct cgaccgcgtc gaggcgccgc
      541 tcgacatcgc cgtgctcacc gactgtccga accccgcgag gaccgagggc ctgctcgagc
      601 aggcgcgtgc caccggcgcc acggtggtca acatcgacca ccaccacgac aaccgtcgct
      661 acggcgacgt caactgggtg gacccggcgg ccgcggccac gggcgagatg gtgcacgacc
      721 tgctgatggc gctcggcgcg ccgctcacgc cggccatcgc caccaatctc ttcaccgccg
      781 tacacatgga caccggctcg ttccggtact ccaacgtcac gcccgacacg ttccggatcg
      841 cgggggcgct ggtcgccgcc ggcgccgatc cagcgttcgt ctccggcgcc ctctacgagc
      901 gccgtccgcg ggaggcgctc cggtggctcg gcgaagcgct ctcgcgcgtg gaggtgagcg
      961 gtgacggccg ggtgggatgg ctcgcgctgc ccgccggtct cgtgcccgag gccttcgtcg
     1021 aggccgaaga cctggtcaac ttcccccgct cggtcgcatc ggtgcgcgtg gcctgcctgc
     1081 tgcgggagcg cgacggcgcg gtgaaggtga gcctccgtgg caagggcgac gtggacgtga
     1141 gccgggtcgc cgcccggttc ggcggcggtg ggcatccgaa cgccgccggc tgcaccgtga
     1201 agacgtcact cgacgccgcc acgcggcagg tgctcgaggc cgttggcgag gcactcggcg
     1261 agcgatgacg gcgtcgcatc ggtcgggcgt gctcgtcgtg gacaaggcgg cggggcccac
     1321 gtccttcgat gtcgtggggc tggtgcgccg gctgttccgg caccgtcggg tcggccacgc
     1381 ggggaccctc gatccgggcg cgacaggcgt cctgcccatc ctcctcggcg aggctaccaa
     1441 gctgatgccg tacctccagg aaatggacaa ggactacgtg acgaccgtca ggctcggggt
     1501 acggacggat acgcaagatc tcaccgggcg cgtgctcgag acggcgccgg tcccgccact
     1561 ggcgcccatc gacgtggagc gcgccgcagt ctccttcgtc ggagccatcc ggcaggtgcc
     1621 tccgatgtac tcggccgtgc atcacggcgg gcgccgcctg tacgagctgg ctcgcgaggg
     1681 gttcgaggtc gcccgcgagg ggcgcgaggt tgtggtccgg gagatcacgg tcgaggaggt
     1741 cgcgctgccg ctcgtcaggc tgcggatcgt gtgcggcaag ggcacctacg tgagaacgct
     1801 cgccgccgac cttggcgagg ccctcgggtg cggtggggcc gtggaggcgc tccgacggac
     1861 gcgagtgggt cccttcacgc tcgacgacgc cgtccccttc tcgatgctca ccgcgatgtc
     1921 ggccgacgcc ctgggggcac gggtccggcc gccgtccgca gcgctcgccg gatggccgac
     1981 cgtgcgcctc gccgagtctg tggcggctcg ctttcgccat ggccaggcgg tggaggtggt
     2041 cactgcccct gtctccggcg cgcgcgtcgc ggtgctgagt gacgacgaca ccctgctcgg
     2101 cgtcggtgag gtcgccgccg ggcgcgtcaa gccggcgcgc atccttcatg cggaccatcc
     2161 ggggcctcga gtcctacccg tctgaggcgc ggccagcggt cgtcgcgctc ggcgtcttcg
     2221 acggcgtcca cctcgcgcat cgcgcgatcc tcgccaaggc ggtcggaagc gcccttgacg
     2281 cgggtctggc gccggtggtc tgcacattcg atccgcatcc catggaagtg ctgcagccgg
     2341 cccgggcgcc gacgcccctc tcgtcgctcg acgagcgcct ggacctgatc gccgccaccg
     2401 gggtacaggc gacgctggtg ttgacattca cgcccgagct ggcggcaatg gagccggagc
     2461 cgttcgtgac ggacgtgctc atcgagcgcc tgcgcgcccg tgaggtggtg gtgggcttca
     2521 accatacgtt cgggcggggg gcgcgggggg acgcgcggct gctccgtgct ctgggtgatc
     2581 gccagggctt cgcaacccac gtggtggagc ctctcgtcgt ggacggtgtc ccggtgtctt
     2641 ccagcgagat ccgtggagcg ctccggggcg gggcggtcga gcgtgcggcg cggtacctgg
     2701 gccggccgta tgcggtggcc ggcgaggt
//
LOCUS       DATIAZ010000005         2421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1448203, whole genome shotgun sequence.
ACCESSION   DATIAZ010000005 DATIAZ010000000
VERSION     DATIAZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448203"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2082)
                     /locus_tag="VLF19_00115"
     CDS             complement(<1..2082)
                     /locus_tag="VLF19_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSE91681.1"
                     /translation="MLSEWPAFQALAKAFAEGEVCLRVAGLGGSARALAVAELHHAHP
                     RQALVLVPSMAEAHQWTQDLAFFGAPAVEFAEREPRLWRGGRQRESDAERALICRHLL
                     GGEPLIVVATPTALGVPLPPAAAFSAGTLRFARGDSLDRELLLEALEAAGYERVDTVV
                     EVGQWSVRGGIVDVFAPTHASPARLEFFGDEIESIRLFDPTSQRSQQTLDELVLLPLE
                     TPPDTALARDAPGATVLDYLAPAAPVIIPSVSVLEERPDEVPERAPLATRLAGRQRIE
                     LELLPGATGPAGFTLETQSVGRYSGRFAQLADELAAWRREGFRVRLAAVDAHQAGHLR
                     QILREHGLEALEVRGLDGPEGLAVVVGECSSGFNIPALGLILLTEGEVFGARRRTLRR
                     PKYQRGSPITAFTDLAVGDLVVHEDHGIGRYLGLRTMTIGDRVGDFLLLEYADGNQLY
                     LPVERLDLISKYLGGDTEAARLDRLGGASWQRVKESVRAALRDMAEELLKLYARRAVA
                     ERKAFSPDVPWQREFEAAFRFEETPDQLKAIGDVKRDMESPRPMDRLVAGDVGYGKTE
                     VALRAAFKAVAEGTQVAVLVPTTVLAQQHYVSFADRFAPFPARVELLSRFRSTAEQKA
                     VVEALKGGSVDVVVGTHRLLSKDVAFKNLGLLIVDEEHRFGVVHKERLKQFRASVDVL
                     AMTATPIPRTLY"
     gene            complement(2221..>2421)
                     /locus_tag="VLF19_00120"
     CDS             complement(2221..>2421)
                     /locus_tag="VLF19_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010689425.1"
                     /note="modulates transcription in response to the
                     NADH/NAD(+) redox state; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HSE91682.1"
                     /translation="GIQIAILAVPAESAQPVTDQLVAAGIRAILNFAPVRIRVPRDVR
                     LKDVDLSIELETLSFYLAKGVR"
BASE COUNT          361 a          885 c          860 g          315 t
ORIGIN      
        1 gtagagcgtg cgggggatcg gcgtcgccgt catcgccagc acgtccacgg aggcgcggaa
       61 ctgcttgagc cgctccttgt ggacgacgcc gaaccggtgc tcctcgtcga cgatgaggag
      121 cccgagattc ttgaacgcca cgtccttgct caacaaccgg tgggtaccga cgacaacgtc
      181 cacgctgccg cccttgagcg cctcgaccac cgccttctgc tccgccgtcg atcggaagcg
      241 cgagagcagc tccaccctgg ccgggaacgg cgcgaagcgg tcggcgaagc tgacgtagtg
      301 ctgctgggcc aggacggtgg tcggcaccag caccgccacc tgcgtgccct cggcgacggc
      361 cttgaacgcc gcgcgcagcg ccacctcggt cttgccgtag ccgacgtcgc cggccaccag
      421 acggtccatg ggtctcggcg attccatgtc gcgcttgacg tcgccgatcg ccttgagctg
      481 gtcgggcgtc tcctcgaatc ggaacgccgc ctcgaactcg cgctgccacg gcacgtccgg
      541 cgagaaggcc ttgcgctcgg cgacggcgcg gcgcgcgtag agcttcagca gttcctcggc
      601 catgtcgcgc agcgccgccc gcaccgactc cttcacgcgc tgccacgagg cgccgccgag
      661 ccggtcgagg cgcgcggcct ccgtgtcgcc gccgagatac ttcgagatca ggtcgagccg
      721 ctcgaccggc aggtagagct ggttgccgtc tgcgtactcg aggagcagga agtcgccgac
      781 gcggtcgccg atggtcatcg tgcggaggcc gaggtagcgg ccgatcccgt ggtcctcgtg
      841 gacgaccaga tcgccgacgg cgaggtcggt gaaggccgtg atcggcgagc cgcgctggta
      901 cttcgggcgg cgcagcgtgc ggcgccgcgc tccgaacacc tcgccttcgg tcaggaggat
      961 cagaccgagc gccgggatgt tgaagccgct cgagcactcg ccaacgacga cggcgaggcc
     1021 ctccggaccg tcgagaccgc gcacctccag ggcttccaga ccgtgctcgc gcaggatctg
     1081 gcgcaggtgg ccggcctggt gggcgtccac cgcggccaga cgcacacgga agccctcgcg
     1141 gcgccaggcg gccagctcgt cggcgagctg cgcgaaccgc ccggagtagc ggcccaccga
     1201 ctgggtttcg agggtgaagc cggccggccc cgtcgcgccc ggcagcagct cgagctcgat
     1261 ccgctggcgg cccgcgaggc gcgtcgccag tggcgcgcgc tcgggcacct catccggccg
     1321 ctcctccagc acgctcaccg acgggatgat cacgggcgcg gccggcgcca ggtagtcgag
     1381 caccgtcgcg cccggcgcgt cccgagccag ggccgtgtcc ggcggggtct cgagcgggag
     1441 gagcaccagc tcgtcgaggg tctgctgcga gcgctgggac gtcggatcga acaggcggat
     1501 ggactcgatc tcgtcaccga agaactcgag gcgtgcgggc gaggcgtgcg tgggcgcgaa
     1561 gacgtccacg atgccaccgc gcacgctcca ctggccgacc tcgaccaccg tgtccacgcg
     1621 ctcgtagccc gcggcctcga gcgcctcgag cagcagctcg cggtcgaggc tgtcccccct
     1681 ggcgaaccgg agcgtgccgg cgctgaaggc ggcggccggc ggcaagggca cgccgagcgc
     1741 ggtcggcgtc gccaccacga tcaggggctc gccgccgagc aggtgccggc agatgagagc
     1801 gcgctcggcg tcggattccc gctgccggcc gccgcgccag aggcgcggct cgcgctcggc
     1861 gaactcgacg gcgggggcgc cgaagaaggc gaggtcctgc gtccactggt gcgcctcggc
     1921 catcgatggg accagcacga gagcctgacg cggatgcgcg tggtggagct cggcgacggc
     1981 aagcgcgcgg gccgatccac cgagaccggc gacacgcaga cagacctctc cctcggcgaa
     2041 ggccttcgcc agggcttgaa acgccggcca ctcactcaac atcgtccgga catccccacc
     2101 ctaccacgcc gactcccggc cgaccgacgc gcgaggcgac caaccgccga tgctcccggc
     2161 accctctcgc tccgatctgc cgtcttcacc gcgcgcgatc acggcgcggg cgccgctgac
     2221 ctagcgcacg cccttcgcga ggtagaagct cagcgtctcg agctcgatcg acaggtcgac
     2281 gtccttgagc cgcacgtcgc gcggcacgcg gatacggacg ggcgcgaagt tcaggatcgc
     2341 gcggatgccc gcggccacga gctgatcggt caccggctgg gcgctctcgg cggggaccgc
     2401 caggatcgcg atctgaatcc c
//
LOCUS       DATIAZ010000006         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_395763, whole genome shotgun sequence.
ACCESSION   DATIAZ010000006 DATIAZ010000000
VERSION     DATIAZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395763"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VLF19_00125"
     CDS             complement(<1..201)
                     /locus_tag="VLF19_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91683.1"
                     /translation="MIATDALSIEEIRRRLSTSRVGAHIYLYGDVASTNATLRTLARS
                     GAPEGTVVLAEGQTAGRGRHGVA"
     gene            complement(198..674)
                     /locus_tag="VLF19_00130"
     CDS             complement(198..674)
                     /locus_tag="VLF19_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE91684.1"
                     /translation="MYKRVIVPLDGSPLAEGILPFILAIAGPLDMDIVLLRVVTPIPP
                     SVIEGSRHVALEDMEGRVADARAYLATVAADLTTRGLRTSVAVRRGEPVVEILAGARD
                     EGADLIAMTTHGRSGLGRVLFGSVAQAVVQHSEVPVFLMKLTKDQMQARAAHGAPR"
     gene            complement(667..1155)
                     /locus_tag="VLF19_00135"
     CDS             complement(667..1155)
                     /locus_tag="VLF19_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE91685.1"
                     /translation="MKVDKILVPLDGSRLSEAALEPALDMMREQPAATLVLLRAVEAT
                     SLPGADATEAQVAVVHEAETYLERVATRLAEVGADRVKTSVWYGPAAASIVEAANVGR
                     VGLIVMSTHGRSGLGRLILGSVAESVVRGTRAPILLVRDQAAPLAPPAGPAKASKEAT
                     HV"
     gene            complement(1312..>2097)
                     /locus_tag="VLF19_00140"
     CDS             complement(1312..>2097)
                     /locus_tag="VLF19_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSE91686.1"
                     /translation="SLPEQLLESELFGHEKGAFTGAIRGKPGLMEVAQGGTLFLDEIG
                     ELSLALQSKLLRALQEREIRRVGGTSQVPVDVRVVSATNRNLREAAARGVFREELYYR
                     VNVIAIELPPLRDRAGDVELLARAFVKKYGQGRVTAIEDAAMTALVRYSWPGNVRELQ
                     NVVERACALTDGATVRRADLPEYLLAGESVRATGAGGGASPVSAATGLPLQEAREHWM
                     QSLETAYLRDVLDRHGGNISAAAKAAGVDRKTFHRLVSKYRIR"
BASE COUNT          287 a          758 c          763 g          289 t
ORIGIN      
        1 ggcgacgccg tgccggcccc ggccggcggt ctggccctcg gcgagcacca cggtgccctc
       61 cggcgcgccg ctccgcgcca gcgtccgcag cgtggcgttc gtcgacgcaa cgtcgccgta
      121 gagatagatg tgggcgccga cccgcgacgt cgagagccga cggcggatct cctcgatgct
      181 gagcgcgtcg gtggcgatca tcgcggtgct ccgtgcgcag cccgggcctg catctggtcc
      241 ttggtgagct tcatcaggaa gaccgggacc tcggagtgct ggaccaccgc ctgcgccacc
      301 gagccgaaca gcactcggcc gaggccgctc cggccgtgcg tggtcatggc gatgaggtcg
      361 gctccctcat cgcgggcgcc ggcgaggatc tcgacgaccg gctcgccgcg gcggaccgcc
      421 accgaggtcc tgaggccccg ggtcgtgagg tccgcggcca ccgtggcaag atacgcgcga
      481 gcgtcggcga cccggccctc catgtcctcg agcgcgacgt gtcgtgaccc ctcgatcacc
      541 gaggggggga tcggggtcac cacgcgcagc agcacgatgt ccatgtcgag agggccggcg
      601 atcgcgagga tgaagggcag gatgccttcc gcgagcggcg agccgtcgag cgggacgatg
      661 acgcgcttat acatgggtcg cctccttgga ggccttcgcg gggccggccg gcggcgccag
      721 cggggccgcc tggtcgcgca cgagcaggat cggcgcccgc gtgccgcgca cgacggactc
      781 ggccaccgag cccaggatca ggcggccgag cccgctgcgg ccgtgggtgc tcatgacgat
      841 cagcccgacg cggccgacgt tcgccgcctc gacgatcgag gccgctgccg ggccgtacca
      901 gaccgaggtc ttcacgcggt cggcgccgac ctcggcgagc cgcgtcgcca cgcgctcgag
      961 gtacgtttcg gcctcgtgca cgacggccac ctgagcctcg gtggcgtcgg cgcccggcag
     1021 gctcgtcgcc tccaccgcac gcagcagcac gagcgtggcc gccggctgct cgcgcatcat
     1081 gtcgagcgcg ggttccagtg cggcttccga cagccgcgag ccgtcgagcg gcaccaggat
     1141 cttgtcgact ttcatgatgg cctcctcgag cgttcgtcgt tcgcttgggt agctaagcaa
     1201 cggcgctgcc atcccgagaa caccggaaat ccaggatgtt ggcgcacgcc cgacgcaccg
     1261 cgcgtggggt ccaaccaccc cgttgggaca gcggcgcccc ggcggcgccc ctcagcggat
     1321 ccggtacttg gagacgagcc ggtggaaggt cttgcggtcg acgccggcgg ccttcgccgc
     1381 cgcggagatg ttgccgccat ggcggtcgag cacgtcacgc aggtaggcgg tctcgagcga
     1441 ctgcatccag tgttcgcggg cctcctggag cggcaggccg gtggccgcgg acacgggcga
     1501 cgcgccgccg ccggcgccgg tggcgcggac ggactcaccg gccaggagat actcgggcag
     1561 atccgcgcgg cgcaccgtgg cgccgtccgt cagcgcgcag gcgcgctcga cgacgttctg
     1621 tagctcgcgg acgttgccgg gccaggagta tcggacgagg gccgtcatcg cggcgtcctc
     1681 gatggcggtg acgcgtccct gtccgtactt cttgacgaac gcccgcgcga gcagctcgac
     1741 gtcaccggcg cgatcgcgga gcggcggcag ctcgatggcg atgacgttga cgcggtagta
     1801 gagctcctcg cggaagacgc cacgggccgc cgcctcgcgc aggtttcggt tggtggccga
     1861 caccacgcgc acgtccaccg gcacctggct cgtgccgccc acccggcgga tctcgcgctc
     1921 ctgcagcgcg cgcagcagct tcgactgcag cgccagcgac agctcgccga tctcgtcgag
     1981 gaacagcgtc ccgccctgcg ccacctccat gagcccgggc ttgccgcgga tggcgccggt
     2041 gaaggcgccc ttctcgtggc cgaagagctc ggactcgagc agctgctccg gcagcga
//
LOCUS       DATIAZ010000007         5334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1711687, whole genome shotgun sequence.
ACCESSION   DATIAZ010000007 DATIAZ010000000
VERSION     DATIAZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711687"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="argJ"
                     /locus_tag="VLF19_00145"
     CDS             <1..942
                     /gene="argJ"
                     /locus_tag="VLF19_00145"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSE91687.1"
                     /translation="DAREIARITGELLRVPARHVLVASTGVIGVPLPMDRIRAALPKL
                     AKALSPQGGRAAAEAIMTTDTRPKEAALRVEVDGRPVTIGAVAKGVGMVEPHLATMFC
                     FITTDAVIGAPTLRRALRRATEGSLNRITVDGDQSTSDTVAALANGLAENAPLDRRGR
                     ALAQFARGLEAITARIARMLVGDGEGATKAVAVAVRGARSRRDALLAARSVASSPLVK
                     TAVNGQDPNWGRIMMALGKSAAKVDQDRVAILFDDETLVERGTLREGVRLDRIRETMG
                     RTEYTITIDLGLGRGEDRVLTCDLSEEYVRINAKYTT"
     gene            1028..1795
                     /gene="rpsB"
                     /locus_tag="VLF19_00150"
     CDS             1028..1795
                     /gene="rpsB"
                     /locus_tag="VLF19_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSE91688.1"
                     /translation="MAALTMKELLEAGVHFGHQTKRWNPKMQKYIFGERNGIYIIDLQ
                     KTLKKFREAYAFVRDLAAQGGAVLFIGTKKQAQDTVFEEANRCGMFYVNQRWLGGTLT
                     NFQTIRKSIGRLKRIEEMKEGGEYDRIPKKEALELDRERQKLEKALIGIKAMEQLPTA
                     VFIVDPRKERIAVAEAQRLGIPIVAIVDTNCDPTGIDYPVPGNDDAIRAVRLITSRVA
                     DAILEGRGTLQKDESAEETAEGASVTDEIAATAASEA"
     gene            1798..2403
                     /gene="tsf"
                     /locus_tag="VLF19_00155"
     CDS             1798..2403
                     /gene="tsf"
                     /locus_tag="VLF19_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSE91689.1"
                     /translation="MASNAQLVKELRDRTGAGVMDCKEALAASRDDLEGAVEYLRKKG
                     VASAAKRAHREVKEGVVGAYIHPGAKLGVLVEIDCETDFVARTDDFQGLVKDLAMQVA
                     AANPAWVAREDVPPAAVEKEREIYREQLADQKKPPQVIDKIIEGKLEKWLGEQCLLEQ
                     PFIRDPSGKTRVKDLVDAVNAKTRERVVVKRFARFQVGEGS"
     gene            2406..3140
                     /gene="pyrH"
                     /locus_tag="VLF19_00160"
     CDS             2406..3140
                     /gene="pyrH"
                     /locus_tag="VLF19_00160"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSE91690.1"
                     /translation="MAADAGSPRPAYRRIVLKLSGEALAGDQGYGIDPAVLERIAAET
                     REVAILGVQVAIVIGGGNIFRGVAASAGGMDRATADYMGMLATIINALALQDALEKAD
                     LQTRVLSAIEMRAVAEPYIRRRAIRHLEKGRVVIFAAGTGNPFFTTDTAGALRAIEIG
                     ADAIMKATKVDGIYSADPKRDAGAERLARTTYAEVLARRLQVMDATAISLCMENRLPI
                     IVFDLTQSGNIKRIVLGEPLGSIVSA"
     gene            3246..3800
                     /gene="frr"
                     /locus_tag="VLF19_00165"
     CDS             3246..3800
                     /gene="frr"
                     /locus_tag="VLF19_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSE91691.1"
                     /translation="MQALVKDIETRMTAAIDTLGREFASVRTGRASTGLLETVRVDYY
                     GTPTPVPQMASVSVPDARTLVIQPWEQAQLKAIEKAIMASDLGLTPINDGKVIRLIMP
                     TPTEERRKQLAKSVHKLAEEARVAIRNVRREANDKLKAMARDKKVSEDEERRGHDQIQ
                     KTTDRFIARVDELLKKKEQEILSF"
     gene            3815..4597
                     /locus_tag="VLF19_00170"
     CDS             3815..4597
                     /locus_tag="VLF19_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HSE91692.1"
                     /translation="MPLQTPNGGSGAELAALDERALLDLVRAHPLPRHVAIIMDGNGR
                     WATRRGLPRVAGHREGVGAARAIVRAAGELGLPYLTLYAFSTENWSRPEDEVTTLMRL
                     LEEAIHRELAELMERNVRLRVIGRGRGVPRPVRRGLDHVVEATRRNTGLNLLIAFNYG
                     GRDELIDAVRAIAQRVARGELAPEAITEADVSDALYTAGAPDPDLLIRTSGEMRVSNF
                     LLWQIAYTELWITPTLWPDFRAADLYHAIADFHGRARRFGGV"
     gene            4599..>5334
                     /locus_tag="VLF19_00175"
     CDS             4599..>5334
                     /locus_tag="VLF19_00175"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSE91693.1"
                     /translation="MPSVTTTVTGAPTAAVKRVLTAVVFLPPFLWMVSRGPGWVFVLF
                     VMAVSGLALWELLRLFQGAGWPVYTRAAIALGVVVTGSFALPAPAVVPSAFLCLAAAV
                     LLSAPLWSGTPPQAEPAALTLVSLVYVSWFLGHVLALHRRPEGDFLVLFLVAVTWLGE
                     TAAYVVGSTIGRHRLAPAVSPRKTMEGAVAQLVVSVAGAGLFAAWLLPAWSLAGALVA
                     GSILGVVGQVGDLVESVMKRSVGVKDA"
BASE COUNT          855 a         1826 c         1939 g          714 t
ORIGIN      
        1 gacgcgcgtg agatcgcgag gatcaccggc gagctcctgc gcgtccccgc ccggcacgtg
       61 ctggtcgcgt ccaccggcgt gatcggcgtg ccgctcccga tggacaggat tcgcgccgcg
      121 ctccccaagc tcgcgaaggc cctgtcgccc cagggggggc gcgcggcggc cgaggcgatc
      181 atgacgaccg acacgcggcc gaaggaggcc gcgctccgcg tggaggtcga cggtcggccc
      241 gtgaccatcg gggccgtcgc caagggcgtg ggcatggtgg agccccacct cgccacgatg
      301 ttctgcttca tcaccacgga cgcggtcatc ggagcgccga cgctgcgccg cgcgctgcgc
      361 cgggcgaccg agggatcgct caaccgcatc accgtggacg gcgaccagtc gaccagcgac
      421 accgtggccg cgctcgccaa cggattggcg gagaacgcgc cgctcgaccg ccgtggccgc
      481 gcgctggcgc agttcgcgcg ggggctggag gccattaccg cgcggatcgc ccgcatgctc
      541 gtgggcgacg gcgagggggc caccaaggcg gtcgccgtgg ccgtccgcgg ggcccgaagc
      601 cgccgcgacg ccctgctggc ggcccgcagc gtggccagct cgccgctggt gaagacggcc
      661 gtcaacggcc aggaccccaa ctgggggcgc atcatgatgg ccctcggcaa gtcggcggcg
      721 aaggtggatc aggatcgcgt ggcgatcctg ttcgacgacg agacgctggt cgagcggggt
      781 accctgagag agggcgtccg gctggaccgg atccgcgaga ccatgggccg gacggagtac
      841 acgatcacca tcgacctggg gctcgggcgg ggcgaggacc gcgtcttgac gtgcgacctc
      901 agcgaggagt acgtgcggat caacgccaag tacaccacgt aacacccatc acgcacgccg
      961 ggcgggactc ggtgcgggga ctccccgcgg gcccggcgga gggcaaaccg aaggagaagg
     1021 aggagacatg gcggcattga ccatgaagga gctgctggag gccggggtgc acttcggtca
     1081 ccagaccaag cggtggaacc cgaagatgca gaagtacatc ttcggcgagc gcaacggcat
     1141 ctacatcatc gatctgcaga agactctcaa gaagttccgc gaggcgtacg cgttcgtgcg
     1201 cgacctggcc gcccagggcg gcgcggtgct cttcatcggc accaagaagc aggcgcagga
     1261 cacggtcttc gaggaggcca accgctgcgg catgttctac gtgaaccagc gatggctcgg
     1321 cggcaccctg acgaacttcc agaccatccg caagtccatc ggacgcctca agcgcatcga
     1381 ggagatgaag gaaggcggcg agtacgaccg gatccccaag aaggaggccc tcgagctcga
     1441 ccgcgagcgt cagaagctcg agaaggccct catcggcatc aaggcgatgg agcagctgcc
     1501 caccgccgtg ttcatcgtcg acccgcgcaa ggagcggatc gccgtggccg aagcccagcg
     1561 cctgggcatc cccatcgtcg ccatcgtgga caccaactgc gacccgacgg ggatcgacta
     1621 tcccgtcccc ggcaacgacg acgccatccg tgccgtccgg ctgatcacct cccgcgtggc
     1681 cgatgcgatc ctcgagggcc gaggcacgct ccagaaggac gagagcgccg aagagacggc
     1741 cgagggcgcg tccgtgaccg acgagatcgc ggccaccgcg gcttcggagg cataggcatg
     1801 gcttcgaacg cccagctggt gaaggagttg cgcgaccgca cgggcgccgg cgtcatggac
     1861 tgcaaggagg ccctggcagc gagccgggac gacctcgagg gcgccgtcga gtatctccgc
     1921 aagaagggag tggccagcgc ggccaagcgg gcccaccgcg aggtcaagga aggggtggtc
     1981 ggcgcgtaca tccacccggg tgccaagctc ggcgtgctgg tggagatcga ctgcgagacc
     2041 gacttcgtgg cgcggaccga cgacttccaa ggcctggtga aggacctggc catgcaggtg
     2101 gcggcggcga acccggcctg ggtcgcccgc gaggacgtgc cgccggccgc ggtcgagaag
     2161 gagcgcgaga tctaccgcga gcagctcgcc gaccagaaga agccgcccca ggtgatcgac
     2221 aagatcatcg agggcaagct ggagaagtgg ctgggcgagc agtgcctgct ggagcagccg
     2281 ttcatcaggg acccgagcgg caagacgagg gtcaaggacc tcgtggacgc ggtgaacgcc
     2341 aagacccgcg agcgggtcgt cgtcaagcgc ttcgcgcgct tccaggtcgg cgagggcagc
     2401 tgagcgtggc ggcggacgcc ggcagcccgc gcccggccta ccgccgaatc gtcctcaagc
     2461 tctcggggga ggcgctggca ggggaccagg ggtacggcat cgacccggcg gtgctggagc
     2521 ggatcgccgc cgagacgcgc gaggtggcga tcctcggcgt ccaggtggcg atcgtgatcg
     2581 gtggcggcaa catcttccgc ggcgtggccg cgagcgccgg cggcatggac cgcgccaccg
     2641 ccgactacat gggcatgctc gccaccatca tcaacgcgct cgcgctccag gatgcgctgg
     2701 agaaggcgga tctccagacc cgggtgctct cggccatcga gatgcgcgcg gtggccgagc
     2761 cgtacatccg gcggcgcgcg atccggcacc tcgagaaggg gcgcgtcgtg atcttcgcgg
     2821 cggggaccgg caacccgttc ttcaccacgg acaccgccgg ggccctgcgc gccatcgaga
     2881 tcggggccga cgccatcatg aaggcgacca aggtcgacgg catctattcc gccgatccca
     2941 agcgcgacgc cggcgccgag cggctcgcgc gcacgacgta cgcggaggtg ctcgcccggc
     3001 ggctgcaggt gatggacgcc accgcgatct cgctctgcat ggagaaccgg ctgccgatca
     3061 tcgtcttcga cctgacgcag tccgggaaca tcaagcgcat cgtgctgggc gagcccctgg
     3121 gctccatcgt ctcggcctag actagggcgt gtcgggatgg cgaggcatgc cacggcgcag
     3181 cacgggcgcg ccgagtgcgg ggagtcgggg gggcccttcg gggccgccgt ggagggattg
     3241 aggccatgca agcgctggtg aaggatatcg agacccgcat gacggcggcg atcgacacgc
     3301 tgggccggga gttcgcctcg gtccgtaccg gccgggcgtc gaccggcctg ctcgagaccg
     3361 tccgcgtcga ctactacggc acgccgacgc cggtgccgca gatggcgtcg gtgtccgtgc
     3421 ccgacgcgcg cacgctcgtg atccagccgt gggagcaggc gcagctcaag gcgatcgaga
     3481 aggccatcat ggcgtccgac ctcgggctca cgcccatcaa cgacggcaag gtcatccgcc
     3541 tgatcatgcc gacaccgacc gaggagcgcc gcaagcagct cgccaagtcc gtccacaagc
     3601 tggccgagga ggcacgcgtg gccatccgca acgtccgccg cgaggcgaac gacaagctca
     3661 aggccatggc cagggacaag aaggtgtccg aggacgagga gcgccgcggc cacgaccaga
     3721 tccagaagac caccgaccgc ttcatcgcgc gggtggatga gctgctcaag aaaaaggagc
     3781 aggagatcct gtccttctaa tacgatgacc agcgatgcct ctccaaacgc ctaacggcgg
     3841 gtccggggcc gagctcgcgg cgctcgacga gcgcgcgctg ctcgacctgg tgcgcgctca
     3901 tccgctgccc cggcacgtgg ccatcatcat ggacggcaac gggcgctggg cgacccgtcg
     3961 cgggctgccc cgcgtggccg gccatcgcga gggcgtgggc gccgcgcgcg ccatcgtccg
     4021 cgcggccggc gaactcgggt tgccgtacct gacgctctac gcgttctcca cggagaactg
     4081 gagccgtccc gaggacgagg tcacgacgct catgcggctg ctcgaagagg cgatccaccg
     4141 cgagttggcc gagctcatgg agcgcaacgt ccggctccgc gtgatcggcc gcggccgcgg
     4201 cgtgccgagg ccggtgcggc ggggcctgga ccacgtggtg gaggccacgc gtcgcaacac
     4261 ggggctgaac ctgctgatcg cattcaacta cggcggccgc gacgagctga tcgacgctgt
     4321 ccgcgccatc gcacaacggg tggcgcgcgg cgagctggcg ccggaggcga tcaccgaagc
     4381 cgacgtgagc gacgcgctct acacggccgg cgcgccggat cccgacctgc tcatccggac
     4441 cagcggcgag atgcgcgtgt ccaactttct cctgtggcag attgcctaca ccgagctgtg
     4501 gatcaccccg acgctgtggc cggatttccg agccgccgat ctctatcacg cgatcgccga
     4561 cttccacggg cgcgcccgtc gcttcggggg ggtgtgaggt gccgtcggtc accacgaccg
     4621 tcacgggcgc gccgaccgcg gcggtcaagc gggtgctcac ggccgtggtg ttcctgccgc
     4681 ccttcctgtg gatggtctcg cgcgggcccg gatgggtgtt cgtgctcttc gtgatggccg
     4741 tgagcgggct cgcgctctgg gagctgctgc ggctcttcca gggagccggc tggcccgtgt
     4801 acacgcgggc ggcgatcgcg ctgggcgtcg tggtgacggg cagcttcgcg ctgccggccc
     4861 ccgccgtggt tccgagcgcg ttcctctgcc tggccgcggc cgtcctgctg agcgcgccgc
     4921 tctggagcgg cacgccgcct caggccgagc ccgcggccct cacgctggtg tcgctcgtct
     4981 acgtgagctg gttcctcggc cacgtgctcg cgctgcaccg gcggccggag ggcgacttcc
     5041 tcgtgctctt cctcgtcgcc gtcacgtggc tcggcgagac cgcggcgtac gtcgtgggct
     5101 ccaccatcgg gcgccacagg ctcgcgcccg cggtcagccc gcggaagacc atggagggcg
     5161 ccgtcgccca gctcgtcgtc tccgtcgccg gcgccgggct cttcgccgcc tggctactgc
     5221 ccgcgtggtc tctcgccggc gccctcgtcg ccggctcgat cctcggcgtc gtcgggcagg
     5281 tgggcgacct cgtcgagtcg gtgatgaagc ggtcggtcgg cgtcaaggac gccg
//
LOCUS       DATIAZ010000008         5515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_396314, whole genome shotgun sequence.
ACCESSION   DATIAZ010000008 DATIAZ010000000
VERSION     DATIAZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396314"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="clpP"
                     /locus_tag="VLF19_00180"
     CDS             <1..597
                     /gene="clpP"
                     /locus_tag="VLF19_00180"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HSE91694.1"
                     /translation="ERAFDIFSRLLKERIIFLPTFIEDELANLVIAQMLFLEAEDPDK
                     DIHLYINSPGGSVTAGMAIYDTMQYVKPAISTICMGQAASMGALLLAAGARGKRFALP
                     HARIMIHQPLGGVQGQATDIDIQAREILRMREELNRILVHHSGQPMDKIQKDTDRDFF
                     MTAEQAKEYRIVDEVISSKPTTRPVTEQAALSAQVQGR"
     gene            620..1870
                     /gene="clpX"
                     /locus_tag="VLF19_00185"
     CDS             620..1870
                     /gene="clpX"
                     /locus_tag="VLF19_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSE91695.1"
                     /translation="MAGRREGNGTLKCSFCGKSQNDVRKLIAGPTVYICDECIELCND
                     IIAEEWEEEKSREIRSLPKPAEIKAVLDQYVVGQDRAKKILSVAVHNHYKRIESGGDA
                     GDVELQKSNILLIGPTGSGKTLLAQTLAKMLRVPFTIADATTLTEAGYVGEDVENIIL
                     RLLQAADYDVERAERGIVYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFIQVDTSNVLFICGGAFVGLDKIIESRVGRSGIGFGAEIKSREER
                     RVGDLLAMIQPEDLLKYGLIPEFVGRLPVIATLHDLDEPALVRILKEPKNAIIKQYQK
                     YFDLEKVRLKFTDDAVAAIAREALKRGTGARGLRAILEEVMLEIMYELPSIQGLKECI
                     ITREVILNREAPMLVSEAKEQQSA"
     gene            1872..2543
                     /gene="rsmI"
                     /locus_tag="VLF19_00190"
     CDS             1872..2543
                     /gene="rsmI"
                     /locus_tag="VLF19_00190"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HSE91696.1"
                     /translation="MSGTLFVVATPIGNLEDLTLRALRVLREVDLVACEDTRRTRALL
                     SRYDVHTPTTSYFEHNKLTRGPQLLRALHEGRSVALVSDAGTPGISDPGFLLVRDARA
                     AGIPVVPVPGPSAVIAALSAAGIPADRFVFDGFPPVKPGKRLNRLRALRALETTVVLY
                     ESPHRILATLEAIGEVFGEVEIVVARELTKQFEEVVAATPAAHLERLRAGTPRGEFTL
                     VIPAP"
     gene            2658..3446
                     /gene="thiD"
                     /locus_tag="VLF19_00195"
     CDS             2658..3446
                     /gene="thiD"
                     /locus_tag="VLF19_00195"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HSE91697.1"
                     /translation="MTVRKALTIAGSDSGGGAGIQADLKTFTAFRVFGMSVLTAITAQ
                     NSLGVQGVFNLPPEFVARQIDSVLSDFGADAVKLGMLSTAPIVEAVADRLAAHGARRV
                     VVDPVMVAKSGDPLLEPEARAALIKRALPLADVVTPNLHEAAVLAGMPVATEKDMEEA
                     ARRIHSLGPRTVLVKGGHLGGAATDILWNGREVTRFTAPRLESSSTHGTGCTFSSAIT
                     ANLARGLALGDAIREAKAYVTAAIREGFQAGRGVGALRHFVERW"
     gene            3451..4206
                     /gene="tatC"
                     /locus_tag="VLF19_00200"
     CDS             3451..4206
                     /gene="tatC"
                     /locus_tag="VLF19_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSE91698.1"
                     /translation="MARAVNDKMSFMEHLGELRMRISRALIGLLIGLAIAFPFSQRIV
                     DHLAQPIQATGNPLVFLAVTEAFWVQMKVALIAGLFFASPVILWQVWAFVSPGLYAHE
                     KKYAAPFVVVGSLLFIGGGAFSLKVVTPYAVQFLLAYARPGLQPMISIGSYVDFLLKF
                     TLAFGLVFELPLAITLAARMGVVTPKTLARNRKYAVLGAFVAAAVLTPTPDAFNQALM
                     AGPLIVLYEVGILCARIFGGRRRPVTATGETPA"
     gene            4206..>5515
                     /locus_tag="VLF19_00205"
     CDS             4206..>5515
                     /locus_tag="VLF19_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSE91699.1"
                     /translation="MDFESLNLPDPVMQGIRAAGFVTATPIQEATLPLALRGKDVAGQ
                     SQTGTGKTAAFLISAFTRFLREPERPGRPPNAPRMLVIAPTRELVVQIESDARQLGRY
                     TPFGIRAVYGGIDYGKQRDSLREGCDFLVGTPGRLIDYLKQHVWSPRRVEVLVIDEAD
                     RMFDMGFIADLRFILRRLPPPEQRQSFLFSATLSFRVLELTWEFMNNPAQISITPQQK
                     TAEKVEQSLYHVGREEKFKLLLGLLKREGGERILIFSNTREEARRLEDRLTRNGWEAR
                     ALTGDVDQKKRLRILNDFKEGRLPVLVATDVASRGLHIEAVSHVINWDLPEDAEDYVH
                     RIGRTARAGAGGKAVALVDEASALRIEPIEKFIGQKISVEWPEDDLFVSEVMPTAEER
                     RRYAEERRARLAARRGRDGRPGDRGRGHRDRGPRPGTPGVERGS"
BASE COUNT          878 a         1948 c         1870 g          819 t
ORIGIN      
        1 gagcgcgcgt tcgacatctt ctcgcggctc ctcaaggagc ggatcatctt cctgcccacg
       61 ttcatcgagg acgagctggc gaacctcgtg atcgcccaga tgctctttct cgaggccgag
      121 gaccccgaca aggacatcca cctctacatc aactcgcccg gcggctccgt gaccgcgggc
      181 atggcgatct acgacaccat gcagtacgtc aagcccgcca tctcgacgat ctgcatgggt
      241 caggcggcgt cgatgggcgc gctcctgctc gctgccggcg cgaggggcaa gcgcttcgcg
      301 ctgccccacg cccgcatcat gatccaccag ccgctcggcg gcgtgcaggg ccaggccacc
      361 gacatcgaca tccaggcgcg cgagatcctc cgcatgcgcg aggagctcaa ccgcatcctc
      421 gtccaccaca gcggccagcc gatggacaag atccagaagg acaccgaccg ggactttttc
      481 atgaccgccg agcaggcgaa ggaatataga atcgtcgatg aagtcatctc gtcgaagccg
      541 acgacccggc cggtgactga gcaggccgca ctgtcggccc aggtccaggg ccgctgagag
      601 cgcggggaag cggagagcca tggcgggcag gcgtgagggg aacggcacgc tgaagtgctc
      661 gttctgcggg aagagccaga acgacgtccg taagctcatc gccggcccga cggtgtacat
      721 ctgcgacgag tgcatcgagc tctgcaacga catcatcgcg gaggagtggg aggaggagaa
      781 gagccgagag atccggagcc ttcccaagcc cgccgagatc aaggccgtgc tcgatcagta
      841 cgtggtcggg caggaccgcg cgaagaagat cctctccgtc gccgtccaca accactacaa
      901 gcgcatcgag tcgggcggcg acgccgggga cgtcgagctg cagaagtcga acatcctcct
      961 gatcggtccc accggctccg gcaagaccct gctggcccag accctggcca agatgctgcg
     1021 ggtgccgttc accatcgccg acgcgaccac gctgaccgag gccggctacg tcggtgaaga
     1081 cgtggagaac atcatcctcc gcctgctgca ggccgccgac tacgacgtcg agcgcgccga
     1141 gcgcggcatc gtctacatcg acgagatcga caagatcgcg cgcaagtccg agaacccgtc
     1201 gatcacccgc gatgtctccg gcgagggcgt gcagcaggcc ctgctcaaga tcctggaggg
     1261 caccgtcgcc aacgtgccgc cgcagggcgg gcgcaagcac ccgcaccagg agttcatcca
     1321 ggtcgacacc tccaacgtgc tgttcatctg cggcggggct ttcgtggggc tcgacaagat
     1381 catcgagtcg cgcgtgggcc gatcgggcat cgggttcggc gccgagatca agagtcgcga
     1441 ggagcggcgg gtcggcgacc tgctggccat gatccagccc gaggacctcc tcaagtacgg
     1501 cctcatcccg gagttcgtcg gccgcctgcc ggtgatcgcc acactccacg atctcgacga
     1561 gccggcgctg gtgcgcatcc tgaaggagcc gaagaacgcg atcatcaagc agtaccagaa
     1621 gtacttcgac ctcgagaagg tgcggctcaa gttcacggac gacgccgtgg ccgccatcgc
     1681 ccgcgaggcg ctcaagcgcg ggaccggggc gcgcgggctg cgggccatcc tggaggaggt
     1741 catgctcgag atcatgtacg agctgccctc gatccagggc ctcaaggagt gcatcatcac
     1801 ccgcgaggtc atcctgaacc gcgaggcgcc gatgctcgtg tccgaagcca aagaacagca
     1861 gtcggcgtag cctgagcggc accctcttcg tcgtcgccac ccccatcggc aacctcgagg
     1921 atctcaccct gcgcgcgcta cgcgtgctgc gcgaggtgga cctcgtcgcc tgtgaggaca
     1981 cgcgccgtac ccgtgcgctc ctgagccgct acgacgtcca cacgcccacg acgtcgtact
     2041 tcgagcacaa caagctgacc cgagggccgc agctgctgag ggcgctccac gaaggccggt
     2101 ccgtcgcgct cgtctccgat gcggggacgc cggggatctc cgacccgggg ttcctgctgg
     2161 tccgcgacgc gcgcgccgcc ggcatccccg tggtgcccgt gcccgggccc tccgccgtga
     2221 tcgcggccct ctcggcggcc ggcatccccg ccgaccgctt cgtcttcgac gggtttcccc
     2281 cggtcaagcc cggcaagcgc ctgaaccggc tccgggcgct ccgggcgctg gagacgacgg
     2341 tcgtgctgta cgaatccccg caccgcatcc tcgccacgct cgaggcgatc ggcgaagtgt
     2401 tcggggaggt ggagatcgtc gtggcacgcg agctgaccaa gcagttcgag gaggtcgtcg
     2461 cggcgacgcc ggcggcccac ctcgagcgcc tgcgcgcggg caccccgcgc ggcgagttca
     2521 ccctggtcat cccggcgccc tgatgggcag cccccgggtg gtcgccgcca tggtcgcgac
     2581 cgtccggccg agaccgcccc gtcgccgtcc ccggcagggg acagcgtgta tactgcgaag
     2641 ggcgacataa aggctatatg acggttcgaa aagcgctgac gatcgccggg tctgactccg
     2701 gcggcggcgc cggcatccag gcggacctca agacgtttac ggcgttccgc gtgttcggca
     2761 tgtcggtcct cacggccatc acggcccaga actccctcgg cgtgcaaggc gtcttcaacc
     2821 tgccgcccga gttcgtcgcc cggcagatcg actccgtgct ctcggatttc ggcgcggacg
     2881 ccgtcaagct cggcatgctc tcgacggccc cgatcgtcga ggctgtcgcc gaccggctcg
     2941 ccgcgcacgg cgcgcggcgc gtggtggtcg atccggtcat ggtcgccaag tcgggcgatc
     3001 cgctgctcga gcccgaggcg cgcgcggcgc tgatcaagcg ggcgctgccg ctcgccgacg
     3061 tggtcacgcc gaacctccac gaggcggccg tgctggccgg catgcccgtg gcgaccgaga
     3121 aggacatgga ggaggcggca cggcgcatcc actcgctggg cccgcgcacc gtgctggtca
     3181 agggcggcca cctcgggggg gccgccaccg acatcctgtg gaacgggcgc gaggtcacgc
     3241 gcttcacggc accgcgcctg gagtcgtcga gcacgcacgg caccgggtgc acgttctcct
     3301 cggccatcac cgccaacctc gcccggggcc tcgccctcgg cgacgccatc cgcgaggcga
     3361 aggcctacgt cacggcggcg atccgcgagg ggttccaggc gggccgcggc gtgggcgcgc
     3421 tgcggcactt cgtggagcgc tggtaggacg atggcgcgcg cggtcaacga caagatgtcg
     3481 ttcatggagc acctgggcga gctgcggatg cgcatctcac gcgcgctcat cggcctcctg
     3541 atcggcctgg cgatcgcctt cccgttcagc cagaggatcg tggaccattt ggcccagccc
     3601 atccaggcga ccgggaaccc gctcgtcttc ctcgccgtga ccgaggcgtt ctgggtccag
     3661 atgaaggtcg cgctgatcgc gggcctgttc ttcgcgtcgc cggtcatcct ctggcaggtg
     3721 tgggccttcg tctccccggg actctacgcc cacgagaaga agtacgcggc cccgttcgtc
     3781 gtcgtcggct cgctgctgtt catcggcggc ggcgcctttt ccctgaaggt ggtgacaccg
     3841 tacgcggtgc agttcctcct cgcctacgcc cggcccgggc tgcagccgat gatctccatc
     3901 ggctcgtacg tcgacttcct cctgaagttc acgctcgcct tcggcctggt cttcgagctg
     3961 ccgctcgcca tcacgctggc ggcgcgcatg ggggtggtca cgccgaagac cctcgcgcgg
     4021 aaccgcaagt acgccgtcct gggggccttc gtggccgcgg cggtcctgac gccgacgccg
     4081 gacgcattca accaggcgct catggccggc cccctgatcg tgctctacga ggtgggcatc
     4141 ctctgcgcgc gcatcttcgg cggcaggcgg cgcccggtca cggcgaccgg cgagaccccg
     4201 gcgtgatgga cttcgagtca ctgaacctcc ccgacccggt gatgcagggt atccgcgcgg
     4261 ccgggttcgt gacggccacg ccgatccagg aggcgacgct gccgctggcg ctccgcggca
     4321 aggacgtcgc ggggcagtcg cagacgggta ccggcaagac ggcggccttt ctgatctcgg
     4381 cgttcacgcg cttcctgcgc gagcccgagc ggcctgggcg gccgcccaac gcgccgcgca
     4441 tgctcgtgat cgcgcccacg cgcgagctgg tggtgcagat cgagtccgac gcccgtcagc
     4501 tcggacgcta cacgccgttc gggatccgcg ccgtctacgg cggcatcgac tacggcaagc
     4561 agcgggactc cctgcgcgag ggctgcgatt tcctcgtcgg cacgccgggc cggctcattg
     4621 actatctcaa gcagcacgtg tggtcgccgc ggcgcgtcga ggtgctggtg atcgacgagg
     4681 ccgaccggat gttcgacatg ggcttcatcg ccgatctccg cttcatcctg cgacggctgc
     4741 cgccgcccga gcaacggcag tcgttcctct tttcggccac gctctcgttc cgggtgctcg
     4801 agctgacgtg ggagttcatg aacaacccgg cacagatctc gatcaccccc cagcagaaga
     4861 ccgcggagaa ggtcgagcaa agcctctatc acgtcggccg cgaggagaag ttcaagctgc
     4921 tgctcggcct cctcaagcgg gagggcggcg agcgcatcct gatcttctcg aacacacgcg
     4981 aggaggcgcg ccgcctcgag gaccgcctca cgcgcaacgg gtgggaggcg cgcgcgctca
     5041 ccggcgacgt cgaccagaag aagcgcctgc gcatcctcaa cgacttcaag gagggacgcc
     5101 tgcccgtgct ggtggcgacc gacgtcgcct cgcgcggcct gcacatcgag gcggtcagtc
     5161 acgtcatcaa ctgggatctc cccgaagacg ccgaggacta cgtccatcgg atcggccgca
     5221 cggcgcgagc cggcgccggc ggcaaggcgg tggcgctcgt ggacgaggcg agcgcgctgc
     5281 ggatcgagcc gatcgagaag ttcatcggcc agaagatcag cgtggagtgg ccggaggacg
     5341 atctcttcgt ctccgaggtc atgccgaccg ccgaggagcg tcgccgctac gccgaggagc
     5401 ggcgggcccg cctcgccgcg cgccgcggcc gcgacggccg gccgggcgac cgcggtcgcg
     5461 ggcatcgcga ccgcgggccg cggccgggta cgccgggcgt cgagcgcggc agcga
//
LOCUS       DATIAZ010000009         8241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1580439, whole genome shotgun sequence.
ACCESSION   DATIAZ010000009 DATIAZ010000000
VERSION     DATIAZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580439"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /gene="urtC"
                     /locus_tag="VLF19_00210"
     CDS             <1..1177
                     /gene="urtC"
                     /locus_tag="VLF19_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HSE91700.1"
                     /translation="CNALPPSSPLHVSNFTLNLFGKLLTYAILALGLDLLWGYAGVLS
                     LGHGVFFGLGAYAMGMHLMLEIGTRSVYQSALPDFMVWNQVTELPLFWRPFHSPLFTL
                     VAVVAVPALAAFVFGFLVFRSRMRGVYLSIVTQAFALCAWLVFNRNEMNLGGTNGLAD
                     FKTLFGFPLSGPATQRGLYVATALALVGAYALCRWITSSRAGKVLVAIRDSETRVLFS
                     GYSPAHYKLFVFVVSATLAGVAGALYAPQVGIITPARIGVLPSIEMVVWVAAGGRGTL
                     VGAVVGAIGVNWTQSWLTTSYPDLWPLFLGALFMSVVLFFPDGVVGAAIRLGRRALSL
                     TTLRGTTAVRSASENHVGHAAPAPRPGAPRADLADPGSVTTPAPHVSGGDARRLARP"
     gene            1174..1956
                     /gene="urtD"
                     /locus_tag="VLF19_00215"
     CDS             1174..1956
                     /gene="urtD"
                     /locus_tag="VLF19_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HSE91701.1"
                     /translation="MTTSAVVYLEDVVVDYDGFKALSHVNFYMDRRELRVVIGPNGAG
                     KTTLLDVISGRVKPASGRVIFGHHTDLVTLRENEIAALGVGRKFQTPSVFANLTVREN
                     VELSLRRSSKGVFATLFGRDGVGDERARIAATLERVGLADRARRRAGTLSHGEKQWLE
                     IGMVIAQEPELLLVDEPVAGMTDDETARTGELLLDIASDRAVLVIEHDMEFVRQIAGK
                     VTVLHQGAVLCEGPVEQVQRDPRVLEVYVGQRRETAAGAGAC"
     gene            1950..2639
                     /gene="urtE"
                     /locus_tag="VLF19_00220"
     CDS             1950..2639
                     /gene="urtE"
                     /locus_tag="VLF19_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HSE91702.1"
                     /translation="MLSVERLDAAYGESPVLWAVDLAVPAGQVVCLMGRNGVGKTTLL
                     RAIMGLMPVRGGRVRFEDHDVTGWSPDRRARAGIGYVPQGREIFPNLTVAENLKMPLL
                     GCGRAAAIDEALELFPALRPLLGRKGGVLSGGEQQMLAIGRALLTRPKLLMLDEPTEG
                     IQPSIILEIEEAIRRIGAEMGLAVLLVEQYLDFAARLADSYVILAKGAVVATGATREL
                     RPEMVRQHLTV"
     gene            2661..2963
                     /locus_tag="VLF19_00225"
     CDS             2661..2963
                     /locus_tag="VLF19_00225"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HSE91703.1"
                     /translation="MHLTPREQEKLLVFTAAEVARRRRARGLKLNHPEAVALITAETL
                     EGIRDGRTVSELMETGLGILCRDDVMEGVPEMIDEVQVEGTFPDGTKLVTIHHPIR"
     gene            3308..3460
                     /locus_tag="VLF19_00230"
     CDS             3308..3460
                     /locus_tag="VLF19_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91704.1"
                     /translation="MEFCILLAYGALAARASAIAVRPRFSALVNRLAGSLLVAAGVGL
                     AQVRRA"
     gene            3501..3854
                     /gene="ureB"
                     /locus_tag="VLF19_00235"
     CDS             3501..3854
                     /gene="ureB"
                     /locus_tag="VLF19_00235"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HSE91705.1"
                     /translation="MIPGEYLLAEGDVVANEGRLTVEVTVTNTGDRPVQVGSHFHFFE
                     VNRALRFDRGMAFGMRLNVPAGTAVRFEPGDARRVTLVEVAGARRVHGLNALTEGGGR
                     EAALARLARWEGSRP"
     gene            3851..5569
                     /gene="ureC"
                     /locus_tag="VLF19_00240"
     CDS             3851..5569
                     /gene="ureC"
                     /locus_tag="VLF19_00240"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HSE91706.1"
                     /translation="MTLRMTRRQYADLYGPTTGDRVRLADTELIVRVERDLTVPGEEA
                     KFGGGKVIRDGMGQSSRATRAEGALDTVVTGALIVDHWGIVKADIGIRDGRIAGIGKA
                     GNPDLMAGVTTGMVIGAATEVIAGEGRIVTAGGLDTHIHFICPQLVAEALSAGLTTMV
                     GGGTGPATGTNATTCTPGEWNLHRMLEAAEAFPVNLGFLGKGNASAPDPLREQIAAGA
                     IGLKLHEDWGTTPAAIDCALGVAEEYDVQVAIHTDTLNEAGFVEDSIRAFKGRTIHTY
                     HTEGAGGGHAPDIIRVCGEPNCLPSSTNPTMPFTVNTMDEHLDMLMVCHHLDPRIPED
                     LAFAESRIRAETIAAEDVLHDLGAISMMSSDAQAMGRIGEVVLRTWQTADKMKRQRGP
                     LRGETPGDDNERVKRYVAKYTINPAIAHGLSDHVGSIEAGKLADLVLWKPAFFGVKPE
                     LVVKGGAIAWAAMGDPSASIPTPQPVLYRPMFGAHGRAPGATALTFVSAAALAADVPR
                     RLGLARRAVAVSRCRGIGKRDMVHNAALPRIEVDPETYEVRADGEVLRCEAAPALPMA
                     QRYFLF"
     gene            5569..6201
                     /locus_tag="VLF19_00245"
     CDS             5569..6201
                     /locus_tag="VLF19_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HSE91707.1"
                     /translation="MRIAVIGAGNVGGALGRVWAQRGHQVVFGVRDPEAGKIRTLVQG
                     AGANATAARIAPAVAGADVVVLAVPWSATGDTIAAAGDLRGKILVDATNPLTPDVSDL
                     AIGHDTSGGEQLARRVPAARVVKAFNTIGAMHLADPTFGGQRASMFLCGDDAAAKATV
                     AELAQAVGFEPVDVGPLRQARLLEPLAMLWISMAVAHGFGPNIAFKLLRK"
     gene            6227..6676
                     /locus_tag="VLF19_00250"
     CDS             6227..6676
                     /locus_tag="VLF19_00250"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HSE91708.1"
                     /translation="MPVVVVAHPHVDPAPGALDGLERDGLVLSAEERRWGRRRVRTRG
                     GRELGLALPTGSVLMPGEVLHVGDGWYVVVEAADEAVIAVTPRSRDEAVRVAFEVGNR
                     HFTLALDGERLLVADDPAMEQLVTRLGVPWRRDRAVFVPIGAGHRHD"
     gene            6795..7460
                     /locus_tag="VLF19_00255"
     CDS             6795..7460
                     /locus_tag="VLF19_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HSE91709.1"
                     /translation="MLQLADGLFPAGGFAHSFGLETYVQARVVGDRAGLEAFVRAHLE
                     ASAGPADAVATACATRIAAGGDLPALAEVDARLDAMKCVPEFRAASRQMGRHTARAAA
                     AFADDPFVSRLASTIAATATPGHHAVVFGAVLGRHGVEPRDAAAAFLHSTAALLVNAG
                     LRLLAIGQVEGQRILAALRPRAAVLAGEAATATVEDMWSFTPGLEIAGLRHADLDARL
                     FRS"
     gene            7457..8068
                     /gene="ureG"
                     /locus_tag="VLF19_00260"
     CDS             7457..8068
                     /gene="ureG"
                     /locus_tag="VLF19_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HSE91710.1"
                     /translation="MIPKPLKIGIGGPVGSGKTALAEALCLRLRDRVEMAVITNDIYT
                     KEDAEFLTRRGALPAERVVGVETGGCPHTAIREDASVNLEAIDGLLRRFPGLDLLLVE
                     SGGDNLAATFSPELVDATVYVIDVAEGEKVPRKGGPGITRSDLLVVNKIDLAPYVGAD
                     LGVMERDARRMRGHRPFVFTNLRTGEGVDRILEWIHSDLLLSA"
BASE COUNT         1095 a         2936 c         3023 g         1187 t
ORIGIN      
        1 ctgcaacgcg ctgccgccct cgtcgccgct ccacgtgtcg aacttcacgc tgaacctctt
       61 cggcaagctc ctgacgtacg ccatcctcgc gctgggcctg gacctgctct ggggctacgc
      121 cggcgtgctg agcctggggc acggcgtgtt cttcggcctc ggcgcctacg ccatgggcat
      181 gcacctgatg ctcgagatcg gcaccaggag cgtctaccag agcgccctgc cggacttcat
      241 ggtctggaac caggtcaccg agctcccgct cttctggcgg ccgttccaca gccccctctt
      301 cacgctcgtg gccgtggtgg cggtgccggc gctggcagcc ttcgtgttcg gcttcctggt
      361 cttccgtagc cgcatgcgcg gcgtgtacct gtcgatcgtg acgcaggcct tcgcgctgtg
      421 cgcgtggctc gtgttcaacc gcaacgagat gaacctcggt ggaaccaacg gcctcgcgga
      481 cttcaagacg ctcttcggct tcccgctgag cgggccggcg acgcagcgcg gcctctacgt
      541 ggccacggcg ctggcgctgg tcggcgccta cgcgctctgc cggtggatca cgtcgtcgcg
      601 ggcgggcaag gtgctcgtgg ccatccgcga cagcgagacc cgcgtgctct tctccggcta
      661 ctcgcccgcg cactacaagc tcttcgtctt cgtggtctcc gcgacgctgg ccggggtggc
      721 cggcgcgctc tacgcgccac aggtgggcat catcacgccg gcgcgcatcg gcgtcctgcc
      781 ctcgatcgag atggtggtgt gggtggcggc gggagggcgg ggcaccctcg tcggagccgt
      841 cgtcggcgcg atcggcgtga actggacgca aagctggctg acgacgagct acccggacct
      901 ctggccactc ttcctgggtg cgctcttcat gagcgtggtc ctcttctttc ccgacggcgt
      961 cgtcggcgcc gccatccgcc tgggacgccg cgcgctctcg ctgacgaccc tgcgcggaac
     1021 caccgccgtt cggtcggcct ccgagaatca cgtcggccac gctgcgcccg cgccccggcc
     1081 gggcgcgccc cgagccgacc tcgcggaccc cgggagcgtg acgacccctg cgcctcacgt
     1141 ctcgggcggg gacgcccggc ggctcgcacg gccatgacca cgtccgccgt cgtctatctc
     1201 gaggacgtcg tggtcgacta cgacggcttc aaggcgctga gccacgtcaa cttctacatg
     1261 gaccgccgag agctgcgcgt ggtgatcggg ccgaacggcg cgggcaagac gacgctgctc
     1321 gacgtgatct ccgggcgggt gaagccggcg agcgggcggg tcatcttcgg ccaccacacc
     1381 gacctcgtga cgctgcgcga gaacgagatc gccgcgctgg gggtgggccg gaagttccag
     1441 acgccgtcgg tgttcgcgaa tctcacggtg cgcgagaacg tcgagctgtc gttgcggcgg
     1501 tcgagcaagg gcgtcttcgc cacgctgttc ggccgcgacg gcgtcggcga tgaacgcgcc
     1561 cgcatcgcgg cgacgctcga gcgcgtcggg ctcgccgacc gggccaggcg gcgggccgga
     1621 acgctgtccc acggcgagaa gcaatggctc gagatcggca tggtgatcgc gcaggagccc
     1681 gagctgctgc tggtggacga gcccgtggcg ggcatgaccg acgacgagac cgcgcgcacc
     1741 ggcgagctgc tcctcgacat cgcgagcgac cgcgcggtgc tggtgatcga acacgacatg
     1801 gagttcgtgc gccagatcgc cggcaaggtg acggtcctcc accagggcgc cgtgctctgt
     1861 gaggggcccg tggagcaggt gcagcgcgat ccccgggtgc tcgaggtcta cgtgggtcag
     1921 cggcgcgaga ccgcggccgg agccggggcg tgctgagcgt ggagcggctc gacgcggcct
     1981 acggagagtc gccggtgctg tgggcggtcg acctcgcggt gccggcgggc caggtcgtgt
     2041 gtctgatggg gcgcaacggc gtgggcaaga ccacgctcct gcgcgcgatc atggggctga
     2101 tgccggtgcg cggtggacgc gtgcgcttcg aggaccacga cgtcaccggc tggtcgccgg
     2161 accggcgggc gcgcgccggc atcggctacg tgccacaggg ccgtgagatc ttcccgaacc
     2221 tcaccgtggc ggaaaatctg aagatgcccc tgctgggatg cggccgcgcg gccgccatcg
     2281 atgaggccct cgagctgttc ccggcgctgc gccctctgct cggccgcaag gggggcgtgc
     2341 tctcgggcgg tgagcagcag atgctggcga tcggccgtgc gctgctcaca cgcccgaagc
     2401 tcctcatgct cgacgagccg accgagggca tccagccgtc gatcattctc gagatcgagg
     2461 aagcgatccg gcggatcggc gccgagatgg ggctggccgt gctgctcgtg gagcagtatc
     2521 ttgacttcgc ggcgcgactg gcggactcgt acgtcatcct ggccaagggc gcggtggtcg
     2581 cgaccggcgc cacgcgcgag ctgcggccgg agatggtcag gcagcacctg acggtgtgat
     2641 tcgatcccgg aggcgcaacg atgcacctca ccccgcgtga gcaggaaaag ctgctcgtct
     2701 tcaccgccgc cgaggtcgcg cggcggcggc gcgcccgtgg cctgaagctg aatcacccgg
     2761 aggcggtggc gctcatcacc gcggagaccc tcgagggcat ccgcgacgga cgcacggtct
     2821 ccgagctcat ggagaccggg ctcggcatcc tctgtcgaga cgatgtcatg gaaggggtgc
     2881 cggagatgat cgacgaggtg caggtcgagg ggacgttccc cgacgggacc aagctcgtga
     2941 ccatccatca cccgatccga tgaacgtcgg cccggccggg gtggcggcgg gggccggcgg
     3001 gtccgccgac ccctgatgcg gtgcgcgtcg tcgggcgcgt gctatttcca tcgacggtgg
     3061 gccggccctg ttcggtcggc gggggacagg tcggcggacc cgccggcccc cgccgccacc
     3121 ccggccggtc accgcgagac ggtcgtcgac gagaggagtc cggcgcggcg gagcgtggtg
     3181 ggcgggggac agatcggccc gggcggccct cgccgtcacc gtgcgagggt cggcgaaacg
     3241 aggagagcct gatggccgtc gaggcgtggc tgctgctcgt cgtcacggcg ccgcgacgag
     3301 cgtggtgatg gagttctgca tactcctggc ctacggggcg ctggccgcgc gcgcgagcgc
     3361 gatcgccgtc cggccccggt tcagcgcgct cgtcaaccgc ctcgccggct cgctgctcgt
     3421 cgccgccggt gtcgggctgg cgcaagtgag gcgcgcgtga ccgccgctcg gccgtggccg
     3481 cctcgcgaga ggagacgggc gtgattcccg gagagtatct gctggccgag ggtgacgtcg
     3541 tggcgaacga agggcggctg accgtcgagg tgacggtgac caacacgggt gaccggccgg
     3601 tgcaggtcgg ctcgcacttc cacttcttcg aggtcaatcg cgcgctccgc ttcgaccgtg
     3661 gcatggcgtt cggaatgcgg ttgaacgttc ccgccggcac ggccgtgcgg ttcgagcccg
     3721 gcgacgctag gcgcgtcacg ctggtggagg tcgccggcgc gcgccgcgtc cacgggctca
     3781 acgccttgac cgagggcggg gggcgcgagg ccgcgctcgc gcggctggcg cgctgggagg
     3841 gctcccggcc gtgaccctcc gcatgacgcg ccggcagtac gccgacctct acgggccgac
     3901 gacgggggac cgcgtgcggc tcgccgacac cgagctgatc gtccgggtcg agcgcgatct
     3961 cacggtgccg ggcgaggagg cgaagttcgg gggcggcaag gtcatccgtg acggcatggg
     4021 gcagtcctcg cgcgccacgc gcgcggaggg cgcgctcgac accgtcgtca ccggcgcgct
     4081 gatcgtcgac cactggggca tcgtgaaggc ggacatcggc atccgcgacg gccgcatcgc
     4141 gggcatcggc aaggccggca acccggacct catggccggg gtcaccacgg gcatggtgat
     4201 cggcgccgcc accgaggtga tcgccggcga ggggcgcatc gtcaccgcgg gcggtctgga
     4261 cacccacatc cacttcatct gcccgcaact cgtggccgag gccctcagcg ccgggctcac
     4321 gaccatggtc gggggcggca ccggtccggc caccgggacc aacgccacga cctgcacgcc
     4381 gggcgagtgg aacctgcacc gcatgctcga ggccgccgag gcctttccgg tgaacctcgg
     4441 cttcctgggc aagggcaatg cgtcggcgcc cgacccgctg cgcgagcaga tcgccgcggg
     4501 agccatcggc ctcaagctcc acgaggactg gggcacgacg ccggcggcga tcgactgcgc
     4561 gctcggcgtg gccgaggaat acgacgtcca ggtggcgatc cacaccgaca ccctgaacga
     4621 ggccggcttc gtcgaggact cgatccgcgc cttcaagggc cggacgatcc acacctacca
     4681 cacggagggc gcgggcggcg gccatgcccc cgacatcatc cgcgtctgcg gcgagccgaa
     4741 ctgcctgccg tcgtccacga acccgacgat gccgttcacg gtgaacacga tggacgagca
     4801 cctcgacatg ctgatggtgt gtcaccacct cgatccgcgg atccccgagg acctcgcctt
     4861 cgccgagtcg aggatccgcg ccgagaccat cgccgcagag gacgtgctgc acgacctcgg
     4921 ggccatcagc atgatgtcgt cggacgcgca ggccatgggg cgcatcggcg aggtggtcct
     4981 ccgcacgtgg cagaccgcgg acaagatgaa gcgccagcgc ggcccgctgc gtggcgagac
     5041 gccgggcgac gacaacgaga gagtcaagcg ctacgtcgcg aagtacacga tcaacccggc
     5101 catcgcccac ggcctcagtg accacgtcgg ctcgatcgag gccggcaagc tggcggacct
     5161 cgtgctctgg aaaccggcgt tcttcggcgt gaagcccgag ctcgtcgtga agggcggggc
     5221 catcgcctgg gcggccatgg gcgaccccag cgcctcgatc cccacgcccc agccggtgct
     5281 gtaccggccc atgttcggcg cccacgggcg ggcgcccggc gcgacggcgc tgacgttcgt
     5341 gtccgcggcc gcgctcgccg ccgacgtgcc gcggcggctc gggctcgccc ggcgcgcggt
     5401 ggcggtgagc cggtgccgcg gcatcggcaa gcgcgacatg gtccacaacg ccgcgctgcc
     5461 gcggatcgag gtcgatcccg agacctacga ggtgcgggcg gacggcgagg tgctccggtg
     5521 cgaggccgcg cccgcgctgc cgatggccca gcgctacttc ctgttctgat gcggatcgcg
     5581 gtcatcggcg ccggcaacgt ggggggcgcg ctcggccgag tgtgggcgca gcgcggtcac
     5641 caggtcgtct tcggcgtgcg cgacccggag gccggcaaga tccggacgct cgttcagggg
     5701 gccggcgcca acgccacggc cgcgcgcatc gctcccgccg ttgccggcgc cgacgtcgtc
     5761 gtgctcgccg tcccgtggtc ggccaccggc gacacgatcg ccgctgccgg cgatctgcgc
     5821 ggcaagatcc tcgtcgatgc gacgaatccc ctcacgcccg atgtctccga cctcgccatc
     5881 ggacacgaca catcaggcgg cgagcagctg gcgcggcgcg tgccggcggc gcgggtggtc
     5941 aaggcgttca acacgatcgg cgcgatgcac ctggccgatc ccacgttcgg gggtcagcgc
     6001 gccagcatgt tcctgtgcgg agacgatgcg gcggccaagg ccaccgtcgc cgagctcgcc
     6061 caggcggtcg gcttcgagcc cgtcgacgtc ggaccgctga ggcaggcgcg cctgctcgag
     6121 ccgctcgcga tgctgtggat ctccatggcg gtcgcccacg ggttcggtcc caacattgcg
     6181 ttcaagctcc tgcggaaata gccgcgccgt ggacgcggca ccggcgatgc cggtcgtcgt
     6241 cgttgcccac ccgcacgtcg atccagcgcc cggcgccctc gacggcctcg agcgcgatgg
     6301 gctcgtgctc tcggcggagg aacgccggtg ggggcgtcgg cgcgtccgga cgcgcggcgg
     6361 ccgcgagctg gggctcgccc tgccgacggg cagcgtactg atgcccggcg aggtcctcca
     6421 cgtcggcgac ggctggtacg tcgtggtcga ggcggcggat gaagcagtga tcgcggtcac
     6481 gccgcgctcc cgcgacgagg cggtgcgcgt ggcgttcgag gtcggcaacc gccacttcac
     6541 gctcgcgctc gacggcgagc gcctgctcgt cgccgacgat cccgccatgg aacagctcgt
     6601 cacgcggctc ggcgtgccgt ggcggcgcga ccgcgccgtg ttcgtcccca tcggcgccgg
     6661 acaccgtcat gactgacccc ttgggggggt ccgacatggt cccccaggtc cccccgacgc
     6721 tcgcggcgcc gcggcacggc cgtggcgtcc ctcgactacg cgattcgcgc tcgggctcgg
     6781 agcgtctcct cgccgtgctc cagctcgccg acggcctgtt cccggccggg gggttcgcgc
     6841 actcgttcgg tctcgagacc tacgtgcagg cccgtgtcgt cggggaccgc gccgggctcg
     6901 aggcgttcgt gcgtgcccac ctcgaggcta gcgcggggcc cgccgacgcg gtcgccacgg
     6961 cgtgcgccac ccgcatcgcg gcaggcggcg atctgccggc cctggccgag gtcgatgccc
     7021 gcctggacgc catgaagtgc gtccccgagt tccgggcggc gagccggcag atgggccgcc
     7081 acaccgcgcg cgcggcggcc gccttcgcgg atgacccgtt cgtctcgcgg ctggcctcga
     7141 ccatcgcggc caccgccacg cccggccatc acgccgtcgt gttcggcgcc gtcctcggcc
     7201 gccacggcgt cgagccgcgg gacgcggcgg cggccttcct gcactccacg gcggcgctgc
     7261 tcgtcaacgc cggcctccgt ctgctcgcca tcgggcaggt ggagggccag cgcatcctgg
     7321 ccgccctgcg tccgcgcgcg gccgtgctcg ccggggaggc cgcgacggcc acggtcgagg
     7381 acatgtggtc gttcacgccc ggtctcgaga tcgcgggcct gcgccacgcc gatctcgatg
     7441 cgaggttgtt ccggtcatga tcccgaagcc cctcaagatc ggcatcggcg gacccgtcgg
     7501 ctccggcaag accgcgctcg cggaggcgct gtgcctgcgc ctgcgcgacc gcgtcgagat
     7561 ggcggtgatc acgaacgaca tctacacgaa ggaggacgcc gagttcctca cccgccgggg
     7621 tgcgctgccg gccgagcgcg tcgtcggcgt ggagacgggc ggctgtccgc acaccgcgat
     7681 ccgcgaggac gcgtcggtca acctggaggc gatcgacggg ctcctgcgcc gcttccccgg
     7741 gctggatttg ctgctcgtcg agagcggcgg cgacaacctc gcggccacgt tcagcccgga
     7801 gctggtcgac gccaccgtgt acgtcatcga cgtggccgag ggcgagaagg tcccccgcaa
     7861 gggcgggcca ggcatcacgc gctcggacct gctcgtcgtc aacaagatcg acctcgcgcc
     7921 gtacgtgggc gccgatctcg gagtcatgga gcgcgacgcc cggcgcatgc ggggccaccg
     7981 gcccttcgtg ttcaccaacc tcaggacggg cgagggcgtg gaccggatcc tcgagtggat
     8041 ccattcggac ttgctgctct cggcatgagc ctcggcgcga ccggcagcgc cccgacggcc
     8101 agtgcgatcg cccgcaccgg acgcgatggg cgcctgcggc tctccctcga gcgatggggc
     8161 accaggaccg tcgtggccgg ctgccgctgg acgcttccgc tccaggtgct ggcgccactg
     8221 gcggtggacg acccggccct g
//
LOCUS       DATIAZ010000010         6810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1186249, whole genome shotgun sequence.
ACCESSION   DATIAZ010000010 DATIAZ010000000
VERSION     DATIAZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186249"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VLF19_00265"
     CDS             <1..1777
                     /locus_tag="VLF19_00265"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91711.1"
                     /translation="FLVVEIMRTPETAEIGKVSSSLMWPERLTLPERVREVVAARLER
                     LGEPAQRLVALAATAGREFDFALLQRSAGLNEQATAEAIEQLVRRRVIQPVGERFDFT
                     HPSVRSVVYGQLPAHRRKELHYRVALAIEELHQDDLKPHHLVLARHLVEAEAWHKALG
                     YLWQAAMQAFGRCAYSEATLLFTQAFDIITRLPKTRPALEQAADVNLAIAHSLLLSGD
                     VAQALVHLREAEFLASAIGDQWRLGWVLAHFSEYFRIAGELDRAIGAGRRALAIGATL
                     GDATLQAALRLQLGQAHHAAGEYREALALLALNVNDETVRGEAAPGEGSLLAALVGHR
                     ARPTLLSAASRTWLILCLAELGDFSQGLGHGWEGIRLAEAASPADVSPLVLAYLGVGR
                     LLLAKGNVKEAIQLLERCRDMHRLGTVESFSLSVDSTLAAAYLVADRLPESVTLSTRA
                     VDEAGSMKVMSGQSLRLTLLGEALLAEGRTEQAREHALRALDLSRRQKERGHEAYALR
                     LVAEVLAQGEAGDLEAAEASYRQAHAIATELEMRPLAALCHLGLARLHRRRDEPHLAR
                     EHLADAVVMCREMERTLAQGPAELQ"
     gene            complement(1897..2595)
                     /locus_tag="VLF19_00270"
     CDS             complement(1897..2595)
                     /locus_tag="VLF19_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91712.1"
                     /translation="MSEGHSDLTDEALGRRLRADLPRHVAPARLRAAVAEAPPRPSRG
                     WWLAPALSAAATALVLLLFFVPLLPRVGPTDPVQRLARAVVSEHTRVALWGARQSEVV
                     PAGTVWLSQETGINLSKIFSGDERLTLVATEPVYLDQRRGVALHYRDADGHHLTYIVL
                     PAPGLGLPERRRVKVDRWRPALLQDDGFSVWVWKQGDLACFIVSDMASETDLAQFKDY
                     FVRLRTATEPKPAY"
     gene            complement(2592..3101)
                     /locus_tag="VLF19_00275"
     CDS             complement(2592..3101)
                     /locus_tag="VLF19_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSE91713.1"
                     /translation="MEADFRREALQHLDALYNFAMYLARKPAEAEDLVQETYLRAFRF
                     SHRFERGTHLRAWLFQILRNTFLTFYRLRERESPLAEDGVPDWDAPMFHEAPDEGPGA
                     LEAHTDLERALQRLPEEFRTVLLLAEVEGMPLEEVAQVMACPVGTVKSRIFRAKERLR
                     TLLRDYGGK"
     gene            complement(3110..3658)
                     /gene="folK"
                     /locus_tag="VLF19_00280"
     CDS             complement(3110..3658)
                     /gene="folK"
                     /locus_tag="VLF19_00280"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSE91714.1"
                     /translation="MARVYLSLGSNLGERVDLLRAAIARLRVTTGIRFVDASCLYETE
                     PWESEPGRTLRERHWYLNCAVAIETSLAPDDLLARLQEIENRLGRERPAGTPEAARFA
                     PRTLDVDILFYDDRVISVPDRLHVPHLLLHERAFVLRPLAELAPELEHPTLYRTIREL
                     LAEVEDEHAVERRDYPARWFED"
     gene            complement(3658..4017)
                     /gene="folB"
                     /locus_tag="VLF19_00285"
     CDS             complement(3658..4017)
                     /gene="folB"
                     /locus_tag="VLF19_00285"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSE91715.1"
                     /translation="MADRLLLDDVRFFGRHGTTKAEEAVGAWFSVDVELAFDLAAAAL
                     SDDLGATIDYGQVARRVVEIGTGQRVNLLERLAGLLADALLREFPAQEVRVRVRKLTA
                     PLEGLVGTPGVMLTRRR"
     gene            complement(4028..4828)
                     /gene="dapB"
                     /locus_tag="VLF19_00290"
     CDS             complement(4028..4828)
                     /gene="dapB"
                     /locus_tag="VLF19_00290"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HSE91716.1"
                     /translation="MTEIVAAGAAGRMGSRIVACLADAPDLRLVAALEAPGHPALGRD
                     AGETAGAGRTGVPLTSDAKAALTRDRVLVEFSTPEATLEHLRLAAAGGARAVIGTTGF
                     SPAQRDEIKALATRAAILVAPNMSVGVTLALRVLALMAKALGDDYDVEITEIHHRWKK
                     DAPSGTALRMAEVVAEALGRDLPGAAVYGRQGMPGERTRGEIGIFSLRSGDVVGEHTV
                     SFGTPGERLELTHRAHSRDTFARGALRAVRFVAGRPPGLYSMLDVLGL"
     gene            complement(4839..5726)
                     /gene="dapA"
                     /locus_tag="VLF19_00295"
     CDS             complement(4839..5726)
                     /gene="dapA"
                     /locus_tag="VLF19_00295"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSE91717.1"
                     /translation="MNRTFQGSLVAMVTPFRQGKVDEAKVRELVEFHVGNGTDALVPC
                     GTTGESPTLDHDEHRRMVELVVEAAAGRIPVVAGTGSNSTAEAISFTRHAERAGAAAA
                     LVVNPYYNRPTQEGLYRHFRAVAEATALPIFVYNIQGRTAVNVETDTMARLARDCKNI
                     VGVKEASGSLDQMTQVILACGPDFIVLSGDDNLTLPLMAVGGRGVISVIANIVPRETA
                     EMTHAALDGDWKRARDLHYKLFPLARAAFIETNPIPIKEAMAMAGMIEPEFRLPMCRM
                     SDANRARLRGIVETYGLVK"
     gene            complement(5871..>6810)
                     /locus_tag="VLF19_00300"
     CDS             complement(5871..>6810)
                     /locus_tag="VLF19_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91718.1"
                     /translation="THRADNTRLRELGVARVFRHESAEGTEPKPAMLSRGRIVGYTEI
                     GTIPLMPGVAPGPPARPGGQVAVVDGTRVTFQDRQAITHGRRYSYVVVTEDLQGRTSP
                     PSPRVSVVFVAPPEAPGQLAATPGEGQVRLTWTPPSRLADGSPAEGPIFYEVLRAPDA
                     TAPLTPVTPAPIEATEIVDRNLENERTYHYAVRALRRQGETTSRGPATERIAATPADM
                     TPPPAPSGLVAAVAGGTVRLSWLPSPASDVARYVIYRAPPGAPFTRVGTVAAPATVFV
                     DRDVPAGTHRYAVTAQDTGSRANEGPRSSEATVTLP"
BASE COUNT          957 a         2371 c         2495 g          987 t
ORIGIN      
        1 cttcctggtc gtcgagatca tgcgtactcc ggagactgcc gagatcggga aggtgtcgag
       61 tagcctcatg tggcctgagc gtctcacgct gcccgagcgg gtgcgggaag tggttgcggc
      121 tcgtctggaa cggctcggcg agccggcgca gcggctggtc gccctggctg ccactgccgg
      181 ccgcgagttc gatttcgccc tgctgcaacg atcggcggga ctcaacgagc aggcgacagc
      241 ggaagctata gagcagctgg tgcgacggcg tgtgatccag ccggtgggag aacgcttcga
      301 cttcactcat ccctcggtcc gcagcgtcgt ctacggtcag ttgccggcac accggcggaa
      361 ggagcttcat tatcgcgtgg cgctggcgat cgaagagctc caccaggacg acctgaagcc
      421 acaccatctc gtgcttgccc gtcacttagt cgaggcagag gcctggcaca aggcgctggg
      481 ctatctctgg caggcggcga tgcaggcctt cggccgctgc gcgtacagtg aggccacgct
      541 gttgttcacg caggcgtttg acatcatcac gcgcctaccg aagacccgcc ccgctctgga
      601 gcaggccgcc gacgtcaacc tcgccatcgc ccactcgctg ctccttagcg gtgatgtcgc
      661 gcaggcgctc gtccacctgc gagaggccga gtttctggcg tcggcgatcg gcgaccagtg
      721 gcgtctcggc tgggtcctgg cccacttcag cgaatacttc cggatcgccg gcgagctcga
      781 ccgggcgatc ggcgcaggcc ggcgggcgct cgcgatcggg gccaccctcg gcgacgcgac
      841 ccttcaggcg gcactacgcc tgcaactcgg acaggcacac catgccgccg gcgagtatcg
      901 cgaggcgctg gccctcctgg ccctcaacgt gaacgacgaa acggtgaggg gcgaagcggc
      961 tccgggcgag gggagcttgc tcgcggccct cgtcgggcac cgggccaggc cgacgctcct
     1021 gtccgcggcg tcccggacct ggttgatctt gtgcctcgcc gagctcgggg acttctcgca
     1081 aggattgggg cacggctggg aaggaatccg gctggccgag gcggcgtcgc cggcggacgt
     1141 ctccccgctg gtgctcgcgt atctcggcgt tggccgtctt ctcctcgcaa aaggaaacgt
     1201 gaaggaagcg atccagctcc tggaacgctg ccgcgacatg caccggctcg gcaccgtcga
     1261 gagcttctca ctctcggtcg actcgacgct tgccgccgcc tatctggtcg ccgatcggct
     1321 gccggagtcc gtgacgctct cgacccgagc cgtggacgag gccggctcga tgaaggtcat
     1381 gtccgggcag tcgctccggc tcacgttgct gggcgaggca ctcctggcgg aaggccgcac
     1441 ggagcaggcg cgagagcacg cgcttcgcgc cctcgacctc tcacggaggc agaaggagag
     1501 ggggcacgaa gcctacgcgc tcaggctcgt cgcggaggtc ctggcgcagg gcgaggcggg
     1561 cgatctcgag gccgcggagg cgtcctatcg tcaggcgcac gccatcgcga ccgagctcga
     1621 gatgcgcccc ctcgcggccc tgtgtcatct ggggctggcg aggctccatc gacgccgcga
     1681 cgagccccac ctcgcgcgtg agcatcttgc cgatgccgtc gtcatgtgcc gggagatgga
     1741 gaggaccctc gcgcagggac cggcagagct tcagtagggc ggcgccgcca gtgcttcggg
     1801 agggccggtg ccgagcagag cgccagccga ccggcgtcgt aagagctcca gcagaggggc
     1861 gccggcagat gccaataggc cggcgccggc cgcgcttcaa tacgccggct tgggctccgt
     1921 cgccgtgcgc agacgcacga agtagtcctt gaactgcgcg aggtcggtct ccgacgccat
     1981 gtccgagacg atgaagcagg cgaggtcgcc ctgcttccac acccacaccg agaagccgtc
     2041 gtcctgcagc agggcggggc gccagcggtc gaccttcacg cggcggcgct ccgggaggcc
     2101 gaggcccggc gccggcagca cgatgtaggt caggtggtgg ccgtcggcgt cgcggtagtg
     2161 cagggcgacg ccccgccgct ggtcgagata gacgggttcg gtcgccacca gcgtcagccg
     2221 ctcgtcgccc gagaagatct tcgagaggtt gatgccggtc tcctggctga gccacaccgt
     2281 gcccgccggc acgacctcgg actggcgcgc gccccacagc gccacgcgcg tgtgctccga
     2341 gacgaccgcg cgcgcgaggc gctggaccgg atccgtcggc ccgacgcgcg gcaggagggg
     2401 gacgaagaac aacagcagca ccagcgccgt ggccgccgcc gacagcgcgg gagccagcca
     2461 ccagccgcgc gaaggccgcg gcggcgcttc ggcgacggcc gcgcgcaggc gggccggcgc
     2521 cacgtgccgc ggcagatcgg cgcgcaaccg ccgccccagc gcctcgtccg tcaggtccga
     2581 atggccctcg ctcattttcc gccgtagtcg cggagcagcg tgcgcagccg ctccttggcc
     2641 cggaagatcc gggacttcac ggtgccgacc gggcaggcca tgacctgcgc gacctcctcg
     2701 agcggcatgc cctcgacctc ggcgaggagg agcacggtgc ggaactcttc cggcagccgt
     2761 tgcaacgccc gctcgaggtc ggtgtgggcc tcgagcgctc ccggaccctc gtccggcgcc
     2821 tcgtggaaca tcggcgcgtc ccagtcgggg acgccgtctt ccgccagcgg ggattcccgc
     2881 tcgcggagcc ggtaaaacgt caagaacgta ttcctcagaa tttgaaacaa ccatgcgcgg
     2941 aggtgagttc cccgctcgaa gcggtgcgag aaccggaagg cccggaggta ggtctcctgc
     3001 accaggtcct cggcctccgc cggcttgcgc gccaggtaca tggcgaagtt gtagagggcg
     3061 tcgaggtgct gcagggcctc gcgccggaag tccgcctcca ccgccggtct cagtcctcga
     3121 accagcgggc ggggtagtca cgccgctcga cggcgtgctc gtcctcgacc tcggcgagca
     3181 gctcgcgaat ggtgcgatag agcgtgggat gctcgagctc cggcgccagc tccgccagcg
     3241 gccggagcac gaacgcgcgc tcgtgaagca gcaggtgcgg gacgtggagg cggtcgggga
     3301 cgctgatgac gcggtcgtcg tagaagagga tatcgacgtc gagcgtccgg ggcgcgaagc
     3361 gcgcggcctc cggcgtgccg gccggccgct cgcggccgag ccggttctcg atctcttgca
     3421 gccgcgccag gagatcgtcg ggcgccagcg aggtctcgat ggcgaccgcg cagttgaggt
     3481 accagtgacg ctcgcggagc gtccggcccg gctcgctctc ccagggctcc gtctcgtaca
     3541 ggcacgaggc atccacgaac cggatgccgg tggtcacgcg cagccgcgcg atcgccgcgc
     3601 gcagcaggtc cacgcgctcg cccaggttcg agccgaggct caggtagacc cgcgccatca
     3661 ccgtcgccgc gtgagcatca cgccgggtgt gccgacgagg ccctcgaggg gggccgtgag
     3721 cttgcgcacg cggacgcgca cctcctgcgc ggggaactcg cgcaggagcg cgtcggcgag
     3781 caggccggcc agacgctcga gcaggttcac gcgctgcccg gtgccgatct cgacgacgcg
     3841 gcgggcgacc tggccgtagt cgatcgtggc gcccaggtcg tcggacagcg ccgccgccgc
     3901 gaggtcgaac gccagctcga cgtccacgga gaaccaggcg ccgaccgctt cctcggcctt
     3961 cgtggtgccg tggcggccga agaagcgcac gtcgtcgagc agcagccggt cggccatcgg
     4021 cgcgggctca gaggcccagg acatcgagca tcgagtagag gccgggcggg cggcccgcca
     4081 cgaaccgcac ggcgcgcagg gccccgcgcg cgaacgtgtc gcggctgtgg gcgcgatggg
     4141 tcagctcgag acgctcgccc ggcgtgccga acgagaccgt gtgctcgccg acgacgtcgc
     4201 cggagcgcag cgagaagatc ccgatctctc cccgggtgcg ctcgcccggc atgccctgcc
     4261 ggccgtacac ggcggcgccg ggcaggtcgc ggccgagcgc ctcggccacc acctcggcca
     4321 tgcggagcgc ggtgccgctg ggggcgtcct tcttccaccg gtggtggatc tcggtgatct
     4381 ccacgtcgta gtcgtcgccg agcgccttcg ccatgagcgc gagcacgcgc agcgcgaggg
     4441 tcacgcccac ggacatgttg ggcgcgacga ggatggccgc gcgcgtcgcc agtgccttga
     4501 tctcgtcgcg ctgggccggg ctgaaccccg tggtgccgat gacggcgcgg gcgccacccg
     4561 ccgcggccag gcgcaggtgc tcgagcgtgg cctcgggcgt ggagaactcg acgagcacgc
     4621 ggtcgcgggt gagggccgcc ttcgcgtccg acgtgagcgg cacgccggtg cgcccggcgc
     4681 cggccgtctc gccggcgtcg cggccgagcg cggggtggcc gggcgcctcg agggcggcga
     4741 cgagccgcag atccggcgcg tcggccaggc aggcgacgat gcggcttccc atccggccgg
     4801 cggcgccggc ggcgacgatc tccgtcacgc cggcgacgct acttgacgag gccgtaggtc
     4861 tcgacgatgc cgcgcaggcg cgcacggttc gcgtcgctca tgcggcacat cggcagacgg
     4921 aactcgggct cgatcatgcc ggccatcgcc atggcttcct tgatggggat ggggttggtc
     4981 tcgatgaacg ctgcgcgcgc cagcgggaag agcttgtagt gcaggtcgcg cgcccgcttc
     5041 cagtcgccgt cgagcgcggc gtgcgtcatc tcggcggtct cgcgcggcac gatgttggcg
     5101 atgaccgaga tcaccccccg gccgcccacg gccatgagcg gcagggtgag gttgtcgtcg
     5161 ccggagagca cgatgaaatc cgggccgcag gccaggatca cctgggtcat ctggtccagc
     5221 gagcccgagg cctccttgac gccgacgatg ttcttgcagt cgcgcgcgag ccgcgccatc
     5281 gtgtccgtct cgacgttgac ggccgtgcgg ccctggatgt tgtagacgaa gatcggcagc
     5341 gccgtggcct cggccaccgc gcggaagtgg cggtagaggc cttcctgggt cggccggttg
     5401 tagtacgggt tcaccaccag cgcggccgcg gcgcccgcgc gctcggcgtg cctggtgaag
     5461 ctgatcgcct cggccgtgga gttcgagccg gtgcccgcca ccaccgggat gcggccggcc
     5521 gcggcctcga cgaccagctc gaccatgcgc cggtgctcgt cgtggtcgag cgtgggcgac
     5581 tcgcccgtgg tgccgcaggg caccagcgca tccgtgccgt tgccgacgtg gaactcgacc
     5641 agctcccgga ccttcgcctc atcgaccttc ccctggcgga agggcgtgac catcgcgacg
     5701 agtgatccct ggaacgttcg gttcatggca cggtctccct ccgccaccag gtcctcgtca
     5761 ggtctcccgg cggcgcacga tcgtgtggct cccatcgagc gtgaggtgat ccatctccgc
     5821 gtcgtatcgg acgtttttct ttgtgaccgt agcatcgggt ctcggggaag tcaaggcagg
     5881 gtgacggtcg cctcgctcga gcgagggccc tcgttcgctc gcgagcccgt gtcctgtgca
     5941 gtgacggcgt agcgatgggt gccggccggc acgtctctgt ccacgaagac ggtcgccgga
     6001 gcggcgaccg tgccgacacg cgtgaacggc gccccgggcg gggcgcggta aatcacgtag
     6061 cgggcgacgt cggacgccgg gctcggcagc cacgacagcc ggacggtgcc cccggccacg
     6121 gcggcgacga ggccgctcgg cgccggcggt ggcgtcatgt ccgcgggcgt cgccgcgatg
     6181 cgctccgtgg cgggaccgcg cgaggtcgtc tcgccctgcc ggcgaagtgc gcgcactgcg
     6241 tagtgatacg tccgttcgtt ctcgaggtta cggtcgacga tctcggtcgc ctcgatcggc
     6301 gccggggtca ccggcgtcaa tggcgccgtg gcgtccggcg ctcgcagcac ctcgtagaag
     6361 atcggtcctt cggccggtga gccgtccgcg agccgcgaag gcggcgtcca ggtgagacgc
     6421 acctggccct cacccggcgt cgccgcgagc tgccccggcg cttcgggtgg cgcgacgaac
     6481 acgacgctga cgcgtggcga cggcgggctc gtgcgcccct ggaggtcctc ggtgacgacc
     6541 acgtagctgt agcggcggcc gtgggtgatc gcctggcgat cctgaaaggt cacgcgcgtg
     6601 ccgtcgacga cggccacctg gcctccgggc cgagcgggcg gtcctggagc cacccccggc
     6661 atgagcggga tcgtgccgat ctccgtgtag ccgacgatgc ggccgcgcga gagcatcgcg
     6721 ggcttgggct cggtgccctc cgcgctctcg tgacggaaca cgcgcgccac gccgagctcc
     6781 cgcagtcgcg tgttgtccgc gcggtgggtc
//
LOCUS       DATIAZ010000011         9482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_923252, whole genome shotgun sequence.
ACCESSION   DATIAZ010000011 DATIAZ010000000
VERSION     DATIAZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923252"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VLF19_00305"
     CDS             complement(<1..563)
                     /locus_tag="VLF19_00305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE91719.1"
                     /translation="MSMYSAPRLYEIAFDMNRKGEVDFLVHCFRRFARGRPVRRVLDI
                     ACGTGPHLVRLADRGYRMSGLDLSPRNIQFVRERLAERGHTGHLVVGDMTDFRLPRPV
                     DAAICMQDSQGHLLTNAQLLAHLRCVARALRRGGLYVFDRYMASSWTNPARSWSWTRR
                     RGNLVVRASFSALEDVDPVSQVFRERLR"
     gene            complement(560..1426)
                     /gene="speB"
                     /locus_tag="VLF19_00310"
     CDS             complement(560..1426)
                     /gene="speB"
                     /locus_tag="VLF19_00310"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HSE91720.1"
                     /translation="MRPVEPRFIACHRPLSEARVVVYGITFEGRVNLRKGADAGPRDL
                     RLASDSIETYSPALRRDLEDLALADAGDCELPDGATPREQLDAARAEIAAWWRPGLIP
                     FMLGGDHTATVPVVEVLGPAFPGLRVLQLDAHPDTREEFLGERFNYASAMARVMDVIG
                     EDRVWQVGIRTGAREEWERKRPHVLPAHEIHPVEAVKRILPELRPHPLYVTIDVDVLD
                     PSEAPGTGSPEPCGITVRDLIEIVRLLEGCRIVGGDLVEVAHAWDPSGRTGIAASWVI
                     REAFLAWWFDAR"
     gene            complement(1453..1932)
                     /locus_tag="VLF19_00315"
     CDS             complement(1453..1932)
                     /locus_tag="VLF19_00315"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HSE91721.1"
                     /translation="MTKRITMAAATVGHAEGGTPLNAFDNALLAAGIGNINLVKVSSI
                     LPPEVPVVDLPKIKPGALIPTAYAAAIGDVPGERLAAAVGYAVPDDPAKNGVIMEFHG
                     HGTRAEAEQAIQAMLEEAFRVRGEPIREMKVVAVEHTVARIGCALAAITLLGEEDLV"
     gene            2708..3103
                     /gene="speD"
                     /locus_tag="VLF19_00320"
     CDS             2708..3103
                     /gene="speD"
                     /locus_tag="VLF19_00320"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HSE91722.1"
                     /translation="MNALGRHLLLELFDCDSDAINNLEAVKGTLIEAAKRAQATIVDV
                     VFHEFNPFGISGVVVIAESHLSIHTWPEYRYAAVDIFSCGDVLQPEVAANYLVEQFGA
                     ERASVVEMQRGMFINAAAPVANKGAVAVS"
     gene            3100..4023
                     /locus_tag="VLF19_00325"
     CDS             3100..4023
                     /locus_tag="VLF19_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSE91723.1"
                     /translation="MTTQQAYKWFLEATTPAEGHMHAILRSLVDTRTKFQHAQIIETP
                     SCGKCLVLDGRMQSTQLDDFIYHEALVHPGLLSVDRRPESALVIGGGEGATLREILKY
                     PSIRRAVMVDIDGEVVALCRRYLPEMHRGAFDDPRTEVRHEDARAYLERTSERFDLIV
                     SDLPEPMEAGPACLLYTREFYRTVHDRLTPTGTLAMQAGMTKACELGFFTAIHSTLRA
                     VFPVVAGYQAFVSCFGTPWGFIVASRKVDPRRLDPAAVDRLVADRVRGSLEYWDGLTH
                     QHAFALPKHIRKAIDAEQRVVTDTEPLIVEA"
     gene            4064..4651
                     /locus_tag="VLF19_00330"
     CDS             4064..4651
                     /locus_tag="VLF19_00330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSE91724.1"
                     /translation="MLILASASPRRLELLERLTREFRVVPADVDETLAGGPTPEAVAA
                     LALRKARTVAVRLEQGVVLGADTVVVIDGDVLGKPADAAEARTMLYRLRGRRHEVITG
                     LAVVDAAGGRSATEAVVTGVVMRGYDDATIDAYVASGEPLDKAGAYAIQATGGALVAG
                     WLGSYSNVVGLPLDATRRLLAGFGVVSAPVSRSRA"
     gene            complement(4620..5603)
                     /locus_tag="VLF19_00335"
     CDS             complement(4620..5603)
                     /locus_tag="VLF19_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE91725.1"
                     /translation="MSIDGVLGVILLLQALAVAVSGPPTSSEYLPLRVADAEGYFEQE
                     GVQVTLRSVRGEVAAAEALATGEVDVAATSLEAILRFGPRVEGRQARLLLGLTAAPPV
                     ALLVSARDRETVRSVRDLAGRRLGYAAPGAPEYAWLAGILAGAGLSVSRVDLVSLGGR
                     RLASALESGDVQAALVHEPAASDLVRDGRARVLVDLHTPDAVGRALGAPTVNAAVFAR
                     QDRLPDAATLEGFVRAVLAAERQIASGPAATLAARLPPPVVGGPEAFARRVETTRDLY
                     LPGGLVSPERLRATVALIRAHMPLPAAVKVPRAEEMIHDEPLRRASGSPAR"
     gene            5785..6444
                     /locus_tag="VLF19_00340"
     CDS             5785..6444
                     /locus_tag="VLF19_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HSE91726.1"
                     /translation="MKPRLLRLHRALLARFGPQRWWPGRSAYEIAVGAVLTQHTAWVN
                     AARAVAALRARGLLRPGRAAALGRAELAAVIRPAGTYRLKALRLAAFTRWLLDRHRGV
                     WATVRRAPLEPLRRELLAVPGLGPETADAILLYAAGRPVFVADAYARRVLSRHRLLDA
                     HAGYEEARAFVQAHLPSDPALFNEFHALLVAVAKSHCRATPRCDGCPLRYDLAGRPPR
                     R"
     gene            complement(6801..7904)
                     /gene="murG"
                     /locus_tag="VLF19_00345"
     CDS             complement(6801..7904)
                     /gene="murG"
                     /locus_tag="VLF19_00345"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSE91727.1"
                     /translation="MLIAGGGTGGHTSPGLAVAAALRDRGVACAWIGSAGGVEARLVP
                     ARGIPYHAIRTGKLRRYWAWQNVVDLGVNVPAGVAAAWRLVARLRPRVVFATGGFVAV
                     PVAAGAAARGIPIVVHEQTAVPGLANRLIARLARRVAVTFPDETAAFAPGQVVVTGNP
                     LRPELRGGSRAEAAARFGLDAALPLVYVTGGALGAHRINRVLGEILEPLLAQAQIVHQ
                     CGDQPATGDRGWLEARRAALPPALARRYTVVPYVGTELAAIYAAAALVLGRAGAGTVN
                     ECCQLGMPALYVPLPGTRGDEQTANARLVERAGGCTVLPQAEMTPARLLERLRALLAE
                     PAALKEMGERARTLAVPDAADRLVRLLLEVAER"
     gene            complement(8032..9153)
                     /locus_tag="VLF19_00350"
     CDS             complement(8032..9153)
                     /locus_tag="VLF19_00350"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HSE91728.1"
                     /translation="MSPLRVGVVFGGRSGEHEVSLASAASVVVALERAGHDVVPIGIA
                     RDGRWIVGGDPLRALAAEARIGLPAGDATGDVKKALAGRAETPLAGHETALTRTEPAG
                     SLPAELRQGLDVVVIMLHGPYGEDGTIQGLFELADVPYVGAGVLASAVGMDKATMKAV
                     FRAHGLPVVDYLVVTRHEWRHEPDAARARVARDIGFPCFVKPSNLGSSVGISKVRDAR
                     ALGPALDEAARHDRKMVVERAVRGREVEVSVLGNDEPVASLPAEIRYTADWYDYATKY
                     AEGQAEVVVPAPIAPELTRRAQELAVAAFRAIDCSGMARVDFFLEGDRLLVNEINTIP
                     GFTATSGYARMWEASGIDYVTLVDRLVHLALERHREKPG"
     gene            complement(9183..>9482)
                     /locus_tag="VLF19_00355"
     CDS             complement(9183..>9482)
                     /locus_tag="VLF19_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91729.1"
                     /translation="AGTVFGQDAGRAEALFIVFAFLFLTPVVFAMGAFVPLFASAVAA
                     EFMFSIARLPQAAGALLFWIALTVTAWVKVDAWLPGVQWLGSLIARAFLIALHAR"
BASE COUNT         1307 a         3443 c         3482 g         1250 t
ORIGIN      
        1 cgcaggcgct cgcggaacac ctggctcacc gggtcgacgt cctcgagcgc cgagaacgag
       61 gcccgcacga cgaggttacc ccgccgccgg gtccacgacc agctccgcgc ggggttcgtc
      121 cacgacgagg ccatgtagcg gtcgaagacg tagagccctc cgcggcggag ggcgcgcgcg
      181 acgcaccgga gatgggcgag gagctgcgcg ttcgtgagga gatgcccctg cgagtcctgc
      241 atgcagatgg cggcatccac ggggcgcggc aggcggaaat cggtcatgtc gcccaccacg
      301 aggtggcccg tgtgaccccg ctcggccagc cgctcgcgga cgaactggat gttgcgcggg
      361 gacaggtcga ggcccgacat ccgatagccg cggtcggcga ggcggacgag gtgcggcccc
      421 gtaccgcagg cgatgtcgag cacgcgccgc accggccgcc cccgcgcgaa gcgcctgaag
      481 cagtggacga ggaagtccac ctctcccttg cggttcatgt cgaaggcgat ctcgtacagg
      541 cgtggggcgc tgtacatgct catcgggcgt cgaaccacca ggcgaggaac gcctcgcgga
      601 tcacccagga ggcggcgatg ccggtgcgtc cgctcgggtc ccaggcgtgg gccacctcca
      661 cgagatctcc cccgacgatc cggcagcctt ccaggagccg gacgatctcg atcaggtcgc
      721 gcacggtgat gccgcacggc tcgggcgagc cggtacccgg cgcctccgac gggtcgagga
      781 catcgacgtc gatggtcacg tagagcgggt gcggacggag ctccggaagg atgcgcttca
      841 cggcctcgac cgggtggatc tcgtgtgccg ggaggacgtg agggcgcttg cgctcccact
      901 cctcccgggc gccggtccgg atgccgacct gccagacccg gtcctccccg atgacgtcca
      961 tcacccgtgc catggccgag gcgtagttga agcgctcgcc gaggaactcc tcgcgcgtgt
     1021 ccgggtgggc gtcgagctgg agcacccgca ggccggggaa ggccgggccc aggacctcga
     1081 cgaccggcac cgtggccgtg tggtctccgc cgagcatgaa ggggatgagg ccgggtcgcc
     1141 accaggcggc gatctccgct cgggcggcat cgagctgctc gcgtggcgtc gcgccgtccg
     1201 gcagctcgca gtcgccggcg tcggccagcg cgaggtcctc gaggtcccgg cgcagagcgg
     1261 gggagtaggt ctcgatcgaa tcggaagcca gccggaggtc gcgcggcccg gcgtcggcgc
     1321 ccttgcggag gttcacgcgg ccctcgaacg tgatgccata gacgacgacg cgagcctcgg
     1381 agagaggccg gtggcaggcg atgaagcggg gctccaccgg gcgcatgccg cctcgacggc
     1441 cgcgagggcc ggctagacca ggtcctcttc gccgaggagc gtgatggcgg ccagcgcgca
     1501 gccgatccgg gccacggtgt gctcgacggc gacgaccttc atctcgcgga tcggctcgcc
     1561 gcggacccgg aaggcctcct cgagcatcgc ctggatggcc tgctcggcct cggcgcgggt
     1621 gccgtggccg tggaactcca tgatgacgcc gttcttggcc ggatcgtcgg gcacggcgta
     1681 gccgacggcc gcggcgaggc gctcgccggg gacgtcgccg atcgccgcgg cgtaggccgt
     1741 cgggatcagc gcgccgggct tgatcttcgg gaggtcgaca acgggaacct cggggggaag
     1801 gatgctcgag accttcacca ggttgatgtt gccgatgccg gccgcgagga gcgcgttgtc
     1861 gaaggcgttg agcggggtgc ccccttccgc atgaccaacc gtcgctgccg ccatcgtgat
     1921 ccgcttcgtc atccgctcgg tcctccccgt gtcgagtctc gcctcccaac cgcgggcgcc
     1981 gtccgcgccg cgcatcatcc gtcactttac gggggtgacc cgccaatgca agagaaaagt
     2041 tgcgttttag gtggctgcgg cggcaggaaa cggatcggag cggcacgact ccgctctcgt
     2101 cggccaccgc catggcgtcg cgtcgggcgc ggcgagcagc ggtcaccggt tcgcacgagc
     2161 cgtctccatt cgaaggcgcg cccacggcgg ctcggcgccg gctgcgccga gcccgatgtg
     2221 agaggtgagc gcgcggctgc tgccttcgcc gggcgggcgc ggcgggctcg cacgaccggg
     2281 aggcggtgtc gccgagctcg cgccgccctc cgctctggcg tcggtgaggc ggcggaggac
     2341 tcggagcgag gtccggcgac gctgagcccg ttcgtcggaa gcgtcgggcg gcgctccgcg
     2401 ggtttccggc ggtggccggc gtgcggtcag ctcgtcagcg gagagggcaa cgcggtcggg
     2461 aggcggcaag tctcgtcgcc cgagcggtgt cgcggctcgc ccgacgccgt cgagcgtcgc
     2521 cgcgagagcg cgaagtgacc gccgccgagc gcaggaaacg tgtttttttg ttgcaatcgc
     2581 cacaccacga ccctacagtg aaacaagttt cggccgcggc gaggtttttc gccgcagaat
     2641 cgcgacgctc gatgcgcgtc gcaggagacg tgcggtagat gaacaggcgg gaagggaggg
     2701 cgctggagtg aacgcactgg ggcgacacct gctgctcgag ttgttcgatt gcgactccga
     2761 cgccatcaac aacctcgagg ccgtgaaggg cactctcatc gaggcggcga agcgggctca
     2821 ggccacgatc gtcgatgtcg tcttccacga gttcaacccg ttcgggatca gcggcgtcgt
     2881 cgtcatcgcc gagtctcacc tgtccatcca cacgtggccc gagtacaggt acgccgcggt
     2941 cgacatcttc tcctgcggcg acgtcctcca gcctgaggtc gcggccaact acctcgtcga
     3001 gcagttcggt gccgagcgcg catcggtggt ggagatgcag cggggtatgt tcatcaacgc
     3061 cgccgcgccc gtcgccaaca agggagcggt cgccgtcagt tgacgacgca gcaggcctac
     3121 aagtggttcc tcgaggcgac gacgccggcc gaggggcaca tgcacgccat cctccgctcc
     3181 ctcgtcgaca cccgcacgaa gttccagcac gcccagatca tcgagacgcc gtcctgcggc
     3241 aagtgcctcg tgctggacgg ccgcatgcag tcgacccagc tggacgactt catctaccac
     3301 gaggccttgg tccacccggg gttgctcagc gtcgaccgcc gtcccgagag cgcgctggtc
     3361 atcggcggcg gtgaaggcgc gacgcttcgc gagatcctca agtacccgtc gatccggcgg
     3421 gcggtcatgg tggacatcga cggcgaggtc gtggccctct gccgccgtta cctgccggag
     3481 atgcaccgcg gcgccttcga cgacccccgc acggaggtcc gccacgagga cgcccgggcc
     3541 tatctcgagc ggacgagcga gcggttcgac ctgatcgtga gcgacctgcc ggagcccatg
     3601 gaggcgggac cggcctgcct cctctacacc cgcgagttct accggacggt tcacgaccgc
     3661 ctgacgccga cgggcaccct cgcgatgcag gcgggcatga cgaaggcgtg cgagctcggc
     3721 ttcttcaccg ccatccacag cacgcttcgg gcggtgttcc cggtggtcgc cggctaccag
     3781 gcgttcgtgt cctgtttcgg cacgccgtgg gggttcatcg tcgcctcccg caaggtcgac
     3841 ccccgccggc tcgacccggc ggcggtggat cgcctcgtcg ccgaccgcgt ccgcggcagc
     3901 ctggagtact gggacggcct gactcaccag cacgccttcg ccttgccgaa gcacatccgc
     3961 aaggcgatcg acgccgagca gcgggtcgtc accgacaccg agcccctcat cgtcgaggcc
     4021 tgaaggcgcc gccgtcacgc cggaccggct ctaggctcca cccgtgctga tcctcgcctc
     4081 ggcttcgccg cggcgactcg agctactcga gcgcctcacc cgcgagttcc gcgtcgtgcc
     4141 cgccgacgtg gacgagacgc tggcgggcgg cccgacgccc gaggcggtcg ccgcgctcgc
     4201 cctgcgaaag gcgcgcacgg tggccgtccg cctcgagcag ggcgtggtgc tcggcgccga
     4261 cacggtcgtc gtgatcgacg gcgacgtgct cggcaagccc gccgacgccg ccgaggcgcg
     4321 cacgatgctg tatcggctcc gtggccggcg ccacgaggtg atcacgggcc tggcggtggt
     4381 cgatgcagcc ggcggacggt cggcgacgga ggccgtcgtc accggagtgg tgatgcgcgg
     4441 ctacgacgac gccacgatcg acgcctacgt cgccagcggc gagcccctcg acaaggcggg
     4501 cgcctacgcg atccaggcga ccggcggggc gctggtggcc ggctggctcg gctcctacag
     4561 caacgtcgtg ggcctgccgc tcgacgccac gcgccggctg ctggcgggct tcggcgtcgt
     4621 cagcgcgccg gtgagccgct cgcgcgcctg agcggctcgt cgtggatcat ctcctcggcg
     4681 cgaggcacct tgacggctgc cggcaacggc atgtgggcgc ggatgagggc gacggtcgcg
     4741 cggaggcgct cgggcgacac gagtcccccg ggcaggtaca ggtctcgtgt cgtctcgacg
     4801 cgccgggcga acgcctcggg gccgccgacc acgggcggcg gcagccgcgc ggccagcgtc
     4861 gccgccggcc cgctggcgat ctgacgctcg gcagcgagga ccgcgcgcac gaagccctcg
     4921 agcgtcgcgg cgtccggcag gcgatcctga cgcgcgaaga cggcggcgtt caccgtcggt
     4981 gccccgagcg cccggccgac ggcgtccggc gtgtgcagat cgacgagcac ccgggcgcgc
     5041 ccgtcacgga ccagatcgga cgctgccggc tcgtggacga gcgccgcctg gacgtctcca
     5101 ctctcgagtg cgctggccag tcggcggccg ccgaggctca caaggtccac ccgcgagacc
     5161 gacagccccg cgcccgccag gatccctgcg agccaggcgt actcgggagc gcccggcgcg
     5221 gcgtacccca gccggcgccc ggcgaggtcg cgcaccgagc ggaccgtctc ccggtcgcgt
     5281 gccgagacca gcagtgcgac cgggggcgcg gccgtgaggc ccaggaggag ccgcgcctgc
     5341 cggccctcga cccgcggccc gaagcggaga atcgcctcga gcgaggtggc ggcgacatcc
     5401 acctcgcctg tcgcgagggc ctcggcagcg gccacctcgc cgcgcaccga gcggagcgtg
     5461 acctggacgc cctcctgctc gaagtagcct tcggcatcgg cgaccctcag cggcaggtac
     5521 tcggatgagg tcggggggcc gctcaccgcg accgccagcg cctgaaggag caggatgacg
     5581 ccgagcacgc cgtcaattga catgggggcc ccgacatggc ccccaagccc cccgacgttc
     5641 ggcacgcccc ggcggagccg tggcgcgcct ctggcacgcg cgacggggat gcgggccccg
     5701 aatggccccc atgtccccag cgcccgccag gcccgttcgt tccggctccg gaggcggacg
     5761 attcccggtc cgccgacgga ccccgtgaag ccacgcctgc tcaggctcca tcgcgcgctc
     5821 ctcgcccgct tcggcccgca gcgctggtgg ccgggccgaa gcgcctacga aatcgcggtg
     5881 ggcgccgtgc tcacgcagca cacggcatgg gtcaacgcgg cgcgcgccgt ggctgcgctg
     5941 cgcgcgcgcg gtctgctgcg ccccggacgc gccgcggcgc tcggccgagc ggagctcgcg
     6001 gccgtcatcc gccccgccgg cacgtatcgc ctcaaggccc tgcggctcgc cgcgttcacg
     6061 cgctggctgc tcgatcgcca tcgcggcgtc tgggcgaccg tccgccgggc gccgctcgag
     6121 ccgctgcgcc gcgagctgct ggcggtgccc gggctgggcc cggagacggc cgacgccatc
     6181 ctcctctacg cggccggacg ccccgtgttc gtcgccgatg cctacgcgcg gcgcgtgctc
     6241 tcgcgccacc ggctgctcga cgcgcacgcc ggctacgagg aggctcgggc cttcgtgcag
     6301 gcgcatctgc cctcggatcc tgcgctcttc aacgagttcc acgccctcct cgtcgccgtc
     6361 gccaagtccc actgccgtgc gacgccccgg tgtgacggct gcccgttgcg ctacgacctc
     6421 gccgggcggc caccgcgacg gtgaccgtga tgctagccgg ttttacgaat cgaagctcgc
     6481 tggcgaaggc gccggccggg gtggggtcgg gggcgtgggt ccggcgaccc ctgatgcgct
     6541 gcgcgtcgtc gatctggtgc tgtttccatc ttcgggtggt ggcggggttc cgtgggcggg
     6601 ggacatgtcg gcggacccac gcccccgacc ccaccccggc ctacggtcgg cgagcttgcg
     6661 actcgagaga ctggagtccc gcattcggct catccggctg ccgctgcgct gctcggccca
     6721 acggagggag aggcgtgcac gcgaagaaga cagcggaatt gcgcgagacg tgtcggtgga
     6781 cggcgccgag ccgcccgttc tcatcgctcc gccacttcca ggagcagacg aacgaggcgg
     6841 tcggcggcgt cgggcacggc gagtgtgcgg gcgcgctcgc ccatctcctt gagtgccgcc
     6901 ggctcggcga ggagtgcccg caggcgctcg agcaggcgag cgggcgtcat ctcggcctgg
     6961 ggcagcacgg tacacccgcc ggcgcgctcc accagcctgg cattggccgt ctgctcgtcg
     7021 ccgcgggtgc cgggcagagg cacgtagagc gccggcatac ccagctggca gcactcgttg
     7081 accgtgcccg ctccggcccg cccgaggacg agcgccgcgg cggcgtagat ggccgccagc
     7141 tcggtgccga catacggcac gacggtatag cgccgcgcca gcgccggagg tagcgccgcg
     7201 cgccgggcct cgagccagcc gcggtcgccc gtggccggct gatcgccgca ctggtggacg
     7261 atctgcgcct gcgcgagcag cggctcgagg atctcgccca gcacgcggtt gatccggtgc
     7321 gcgcccagcg cgccgccggt cacgtagacg agcggcagcg cggcgtcgag gccgaaccgg
     7381 gcggccgcct ccgcgcgcga gccgccccgg agctcggggc gaagtgggtt cccggtgacg
     7441 acgacctgcc cgggcgcgaa cgcggcggtc tcgtccggaa acgtgacggc cacgcgccgg
     7501 gccagacgcg cgatgagccg gttggcgagc ccgggcacgg ccgtctgctc gtggacgacg
     7561 atgggaatgc cccgcgcggc ggccccggcg gccacgggca cggcgacgaa gccgcccgtg
     7621 gcgaagacca cgcgcgggcg caggcgggcg acgagtcgcc acgccgccgc gacgccggcc
     7681 ggcacgttga cgccgagatc gacgacgttc tgccacgccc agtagcgccg cagcttgccg
     7741 gtgcggatgg cgtggtaggg gatcccgcgc gcgggcacca ggcgcgcctc gacgccgccg
     7801 gcgctgccga tccacgcgca cgcgacgccg cgatcacgga gggcggccgc gaccgcgagc
     7861 cccgggctcg tgtggccgcc ggtaccgccg ccggcgatga gcacggtcgg gcggtctatt
     7921 gccgtcggag cgcctccgcc gggccatcgc cgaccatttc agcgctcggc tcggccgccg
     7981 agccggcctg cgcctcgcac cgtggctcga tcggcccggc gggccacgca cctaccctgg
     8041 cttctcgcgg tggcgttcca gcgcgagatg cacgaggcga tccacgagcg tgacgtagtc
     8101 gatccccgac gcctcccaca tgcgcgcgta gccgctcgtc gccgtgaagc cggggatggt
     8161 gttgatctcg ttcacgagga gccgatcgcc ctcgaggaag aagtccacgc gcgccatgcc
     8221 gctgcagtcg atggcgcgga aggccgcgac ggcgagctcc tgcgcgcggc gggtcagctc
     8281 gggcgcgatg ggcgcgggca cgacgacctc cgcctggccc tcggcgtact tcgtggcgta
     8341 gtcgtaccag tccgcggtgt agcggatctc cgccggaagg gaagccacgg gctcgtcgtt
     8401 gcccagcacg ctgacctcga cctcgcgccc gcggacggcg cgctccacca ccatcttgcg
     8461 gtcgtggcgc gcggcctcgt cgagcgccgg gccgagcgcc cgggcgtcgc gcaccttgct
     8521 gatgcccacg ctcgagccga ggttcgacgg cttgacgaag cagggaaagc cgatgtcccg
     8581 cgcgacccgc gcccgcgcgg cgtcaggctc atggcgccac tcgtggcgcg tcaccacgag
     8641 gtagtccacg acgggcagac cgtgggcgcg gaacaccgcc ttcatcgtgg ccttgtccat
     8701 gccgacggcg gaggcgagca cgccggcgcc cacgtagggg acgtcggcca gctcgaagag
     8761 tccctggatc gtcccgtcct cgccgtaggg cccgtggagc atgatgacga cgacgtcgag
     8821 gccctggcgc agctccgcgg gcaggctgcc cgcgggctcg gtgcgcgtga gcgccgtctc
     8881 gtgtccggcc aggggtgtct cggcgcggcc ggcgagcgcc ttcttcacgt cgcccgtcgc
     8941 gtcgccggcc gggagcccga tgcgggcctc ggcggcgagc gctcgcagcg ggtcgccgcc
     9001 gacgatccag cggccgtcgc gcgcgatgcc gatgggcacg acgtcgtggc cggcgcgctc
     9061 cagcgcgacg acgacggagg ccgccgaggc cagcgacacc tcgtgctctc cggagcggcc
     9121 gccgaagacg acgccgaccc gcagcggcga catgttgcga ttctagcagg cgcgcgcggt
     9181 ggtcaccgcg cgtgcagcgc gatgaggaac gcccgcgcga ttaggctgcc gagccactgg
     9241 acgcccggga gccaggcatc caccttcacc cacgccgtga ccgtgagcgc gatccagaag
     9301 agcagcgcgc ccgcggcctg cggcaggcgc gcgatcgaga acatgaactc ggcggccacc
     9361 gcggaggcga acaaggggac gaacgcgccc atcgcgaaca ccaccggcgt gaggaagagg
     9421 aaggcgaaca cgatgaacag cgcctcggcc cggccggcgt cctgtccgaa gaccgtcccc
     9481 gc
//
LOCUS       DATIAZ010000012         4171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_660445, whole genome shotgun sequence.
ACCESSION   DATIAZ010000012 DATIAZ010000000
VERSION     DATIAZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660445"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..974)
                     /locus_tag="VLF19_00360"
     CDS             complement(171..974)
                     /locus_tag="VLF19_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSE91730.1"
                     /translation="MPPADRPPGRPPGKASRGSPLPGAGALTLVAVAVLAAALVKGAI
                     GFGFPTLATPLLTLFIDVKTAVVVLILPNIVMDGLQFSRRGAPAATMRRFAWLLVFGA
                     LGTVLGTRVLVTLSSRTATLTLAAFLLVFVVLNAARVSPRVPSPWEPWLSPLVGLIAG
                     IVGGVTNVPGTPLAMYFYALRLPKHEFVQSIAFTFLVYKTIQLGAVAYYGLLTWTSLG
                     VAVGLTAVALAGFAVGLRVQDRLAPAAFDRAVLAFLALLGAWLLARTLW"
     gene            complement(893..3799)
                     /locus_tag="VLF19_00365"
     CDS             complement(893..3799)
                     /locus_tag="VLF19_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSE91731.1"
                     /translation="MSDLEHELRKVVAGDVRFDTGSRLLYSTDASMYQVEPVGVVIPR
                     DRDDVEAAMTVARTHGVALLPRGGGTSLTGQTVNHALVLDFSRWMNQVLEVNETERWA
                     RVQPGLVQDELNHHVRPQGLLFGPDTSTSNRATLGGMIGNNSGGSHSIAYGLTVDHVI
                     EVTALLADGTRVVVGDVTPDEFARKSALDGAEGRIYREVARIRDTYADEIRARYPNHW
                     RRVAGYNLNELVGIGVRPGSYAGGGNGARRPFSLARLVVGSEGTLLTVVEAKVRLVPR
                     PKHTALAVIHYRDMQEALESSQSILETGAHAVELTDKMILDLARDNIEQRKRAGFIQG
                     DPAAILIVEYTGDSAAEARGKVEALEARRQRERFGYAAHQAFDPAEQQSIWKLRKAGL
                     GLLFGLKGDGKPIAFVEDTAVDPKHLPEFVPRFRRVFARHGTDGAYYGHCSVGCLHIR
                     PVINLKTKRGLEQVEAIAREITDLVLEFGGTISSEHGDGRARSPLLERMYGPTLMRAF
                     RELKAAFDPEGRMNPGNIVANSDLTRHLRYGAQYTTWEPRTLLDFSEQGGFAAAVEMC
                     NGVGECRKKLEGTMCPSYMATRDEEHSTRGRANALRAVLSGKLPPTEFTGTRLYEVMD
                     LCLECKGCKAECPANVDMAKLKYEFLHHYHRAHGLPLRNRLFGHIARLNALGSRVPGL
                     VNRISRLAPNRWLLEKVAGIDRRRPLPTLAPETFTAWFARRPAPAAAPRGEVVLFHDT
                     FTTYNVPEIGRAAVALLEAGGYRVVLADRKCCGRPLISKGMLDVAREHAAWNVARLHP
                     HVARGAAIVGLEPSCLLTLRDETVDLVRTDEARAVARASFLLEEFLVRERDRGLTLDF
                     ARHGRKALLHGHCHQKALVGTAPTVTALRWAGYEVSEVDSGCCGMAGSFGFEREHYDV
                     SAWLGNRRLVPAVKAAPADTEIVAPGMSCRQQIDHLAGRRAKHPAEALCQALAR"
     gene            3819..>4171
                     /locus_tag="VLF19_00370"
     CDS             3819..>4171
                     /locus_tag="VLF19_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91732.1"
                     /translation="MRGVIRLVPVLARLGLIVVFVLAAVGTASPRLIVSGPPPAARAA
                     ALRGPAVGRITLVGAAEAAPAAPGFRVKVLDSSRYVDTRELIGKRVLVLRFQSSYCKP
                     CGRESAGLDRIAARYR"
BASE COUNT          596 a         1461 c         1534 g          580 t
ORIGIN      
        1 ttccaggcct gagtggccga ccaggcgtcg taagactggc ggtagtcggc gtggaaggca
       61 tggggcggat ccgggccagg ggttctcccg gaccggacca cgggcgtcga ccagcctgaa
      121 gagcgcggga tcgcggcgct cctcggcacg gcgaccggtc cggcgccctg tcaccacagg
      181 gtgcgggcca gcagccacgc accgagcaac gcgaggaagg cgagcaccgc gcgatcgaac
      241 gccgccggcg cgagccgatc ctggacccgc aggcccacgg cgaacccggc gagggcgacg
      301 gccgtgagcc cgacggccac gccgagcgac gtccacgtca gcaggccgta gtacgcgacg
      361 gctccgagct ggatggtctt gtagaccagg aaggtgaagg cgatcgactg aacgaactcg
      421 tgcttgggca ggcgtagcgc gtagaagtac atcgccagcg gcgtgccggg cacgttcgtc
      481 acgccgccga cgatgcccgc gatgagcccc accagcggtg acagccacgg ctcccacggc
      541 gaggggacgc ggggggacac ccgcgcggcg ttgaggacca cgaagacgag gaggaaggcg
      601 gccagcgtga gggtcgccgt tcgcgacgac agcgtgacga ggacccgcgt gccgagcacg
      661 gtgccgagcg caccgaacac gaggagccac gcgaaccggc gcatggtggc ggccggcgcc
      721 ccgcgtcgcg agaactggag gccgtccatc acgatgttgg ggaggatgag gaccacgacg
      781 gccgtcttca cgtcgatgaa gagcgtcagc agcggcgtcg ccagcgtcgg gaacccgaag
      841 ccgatggcgc ccttgacgag cgccgcggcg aggacggcga ccgcgacgag ggtcagcgcg
      901 ccagcgcctg gcagagggct tccgcgggat gctttgcccg gcggcctgcc aggtggtcga
      961 tctgctggcg gcaggacatc ccgggcgcga cgatctcggt gtcggcgggg gccgccttca
     1021 ccgcgggcac cagacggcgg ttgccgagcc aggcgctgac gtcgtagtgc tcgcgctcga
     1081 agccgaagga gccggccatg ccgcagcagc cggagtcgac ctcgctgacc tcgtagccgg
     1141 cccagcgcag cgcggtcacg gtgggcgccg tgcccacgag cgccttctgg tggcaatgac
     1201 catggagcag ggccttgcgc ccgtggcggg cgaagtccag ggtcaggccg cggtcacgtt
     1261 cacggacgag gaattcctcg agcaggaacg acgcgcgcgc cacggctcgg gcctcgtcgg
     1321 tgcgcaccag atccaccgtc tcgtcgcgaa gcgtcagcag acacgagggc tccagcccga
     1381 cgatggccgc gcccctggcc acgtgggggt gcaggcgcgc gacgttccac gccgcgtgct
     1441 cgcgggcgac gtccagcatc cccttggaga tcagcgggcg gccgcagcac ttgcggtcgg
     1501 ccagcaccac ccggtagccg ccggcctcga gcagcgccac cgcggcgcgg ccgatctcgg
     1561 ggacgttgta ggtcgtgaac gtatcgtgga acagcaccac ctcgccgcgc ggcgccgccg
     1621 ccggcgcggg ccggcgcgcg aaccaggccg tgaacgtctc gggcgcgagc gtcggcagcg
     1681 gacggcgccg gtcgatgccc gcaaccttct cgagcagcca ccggttgggc gcgagccggg
     1741 agatccggtt gacgagcccg ggcacgcggc tgcccagcgc gttcagccgg gcgatgtggc
     1801 cgaagagccg gttgcgcagc ggcaggccgt gggcccggtg gtagtggtgg aggaattcgt
     1861 acttgagctt cgccatgtcc acgttggccg gacattcggc cttgcagccc ttgcactcga
     1921 ggcagagatc catgacctcg tagagccgcg tgccggtgaa ctccgtgggc ggcagcttcc
     1981 ccgagagcac cgcccggagc gcgttggcgc ggccgcgcgt ggagtgctcc tcgtcccgcg
     2041 tggccatgta ggacgggcac atcgtgccct cgagcttctt gcggcactca cccacgccgt
     2101 tgcacatctc gaccgcggcg gcgaagccgc cctgctcgga gaagtcgagc agcgtcctcg
     2161 gctcccacgt cgtgtactgg gcgccgtagc gcaggtgccg cgtgaggtcg gagttcgcga
     2221 cgatgttgcc ggggttcatc cgcccctcgg gatcgaaggc ggccttcagc tctcggaacg
     2281 cccgcatgag cgtcggaccg tacatccgct cgaggagcgg gctgcgcgcg cgtccgtcgc
     2341 cgtgctcgct ggagatggtg cctccgaact ccagcacgag gtcggtgatc tcacgggcga
     2401 tggcttccac ctgctcgagc ccgcgcttcg tcttgaggtt gatgaccggg cggatgtgca
     2461 ggcaccccac ggagcagtgg ccgtagtagg cgccgtccgt gccgtgccgc gcgaagaccc
     2521 ggcggaaccg ggggacgaac tccggcaggt gcttgggatc caccgcagtg tcctcgacga
     2581 aggcgatcgg cttgccgtca cccttgagcc cgaacagcag gccgagcccg gccttgcgga
     2641 gcttccagat cgactgctgc tcggcggggt cgaacgcctg gtgcgccgcg tacccgaacc
     2701 gctcgcgctg gcgacgggct tcgagcgcct cgaccttgcc gcgcgcctcg gcggcgctgt
     2761 cccccgtgta ctcgacgatg aggatggccg ccggatcgcc ctggatgaac ccggcgcgct
     2821 tgcgctgctc gatgttgtcg cgcgcgaggt cgaggatcat cttgtcggtc agctcgacgg
     2881 cgtgggcgcc ggtctcgagg atcgactggg acgactcgag ggcctcctgc atgtcgcggt
     2941 agtggatcac ggcgagcgcc gtgtgcttcg gccgcggcac gagccgcacc ttggcctcga
     3001 cgacggtgag cagcgtgccc tcggagccca ccacgagccg cgccagactg aagggccgcc
     3061 gggcgccgtt gcccccgccc gcgtaggagc caggcctcac gccgataccc accagctcgt
     3121 tcaggttgta gccggccacg cgccgccagt gattcggata gcgcgcgcgg atctcgtcgg
     3181 cgtaggtgtc gcggatgcgc gccacctcgc ggtagatccg gccctcggcg ccgtcgagcg
     3241 cgctcttgcg ggcgaactca tccggcgtca cgtcgccgac gaccacgcgg gtgccgtcgg
     3301 ccaggagcgc cgtgacctcg atcacgtggt ccaccgtgag gccgtaggcg atcgagtgcg
     3361 agccgccgga gttgttcccg atcatgccgc cgagggtggc ccggttcgac gtcgaggtgt
     3421 cgggcccgaa gagcagcccc tgcggccgca cgtggtggtt cagctcgtcc tgcacgagcc
     3481 ccggctgcac gcgcgcccac cgctccgtct cgttcacctc cagcacctgg ttcatccagc
     3541 gcgagaagtc gaggaccagc gcgtgattga ccgtctggcc ggtgagcgag gtgccgccgc
     3601 cccgggggag cagcgccacg ccgtgcgtgc gcgccacggt catggccgcc tcgacgtcgt
     3661 cgcggtcgcg cgggatcacg acgcccacgg gctccacctg gtacatcgag gcgtcggtcg
     3721 agtacagcag ccgggacccg gtgtcgaagc ggacgtcgcc agcgacgacc ttgcggagtt
     3781 cgtgctcgag gtcgctcacc gggaggtatt atacgcgcgt gcgcggcgtg atccggctcg
     3841 tccccgtgct cgcgcggctc gggctcatcg tggtcttcgt gctcgccgcc gtgggcaccg
     3901 cctcgccgcg cctgatcgtg tcaggcccgc ctccggcggc gcgcgcggcc gcgcttcgcg
     3961 gtccggcggt cgggcggatc acgctcgtcg gcgccgccga ggcagcgccg gcggcgccgg
     4021 gcttccgcgt gaaggtcctc gacagctcgc gctacgtgga cacgcgcgag ctcatcggca
     4081 agcgcgtcct cgtgctgcgc ttccagtcgt cgtactgcaa gccgtgcggc cgcgagtcgg
     4141 ccggcctcga ccgcatcgcc gcccgctacc g
//
LOCUS       DATIAZ010000013         4789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_2711, whole genome shotgun sequence.
ACCESSION   DATIAZ010000013 DATIAZ010000000
VERSION     DATIAZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VLF19_00375"
     CDS             <1..699
                     /locus_tag="VLF19_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSE91733.1"
                     /translation="GADTARLFSLFAAPPEKDLDWNDHGVEGAFRFLNRVWRFVTAHL
                     DELAPGPAVAAAATLSPDGRAFRRTIHETIRRVTGDIERDFHFNTAVSAIMELVNALQ
                     AFEASTLDRLPAGERRALMCEAVDTLVLLLAPFCPHVTEELWERLGRAGGVVAQRWPE
                     ADPAALVKDEVTVVVQVDGKVRSRLTVDVGASEAEVRRQALEDDKVRPWLGARTIDRV
                     VVVPNRLVNIVTRS"
     gene            696..1211
                     /locus_tag="VLF19_00380"
     CDS             696..1211
                     /locus_tag="VLF19_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HSE91734.1"
                     /translation="MTRHGAFPAVVLVLLGALAGGCGYSLRGTLPDHLKTIAVPVFAN
                     RTREPFVENIVTRAVVEAFTTSGRLRVVRPEEADSILEGEVTGYDLQSVAFDSAANVR
                     QFRLVVTMNLRFRDLRENKVLYDRAGVQERADFRVPASVSDTIAREQSALQVAAVEIG
                     RAVVSFTIERF"
     gene            1274..2245
                     /gene="holA"
                     /locus_tag="VLF19_00385"
     CDS             1274..2245
                     /gene="holA"
                     /locus_tag="VLF19_00385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSE91735.1"
                     /translation="MPPIVLVHGADAQSLDDTLAAVTRALFTNPSSTAFDREVFDGRE
                     TDPEAIVTAALTLPLAAPRRLVAVRRAQAVPARAAEALARYVAAPNPSACLLLLADEA
                     LHPSRDRRTAHWLLGVVPPAAVVAPALRRGRAVEEWLRARVQVEGLTIGADAARLLVQ
                     WVGDDGATLLAEARKAALAGGPDNCAVGVKEVEAVVGEHKVSGVFDLTRAIERREVGL
                     ALRTLERLLATEDPMPVLVMLTREVRTAWSVREWHAAGQSVEQMARRLGRPPAVVQAV
                     VTTAAGQSPAALARKLALCWHAERRLKSGGEARAEMTALVAELARRG"
     gene            2238..3551
                     /locus_tag="VLF19_00390"
     CDS             2238..3551
                     /locus_tag="VLF19_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSE91736.1"
                     /translation="MARACGAAGILAVLLLATRVSAADACPACLRAGAASVALRVPAG
                     APLAGYGSLSRRLLLPDVLGRHAHAFWFKPHDGALDEVRARALVVETATVRVVWVTAD
                     LIAVGREFTARLARRLGEIGVPPGRLLLSASHTHSGPGAFIDSAVMGFISLDRVDEGV
                     REALIGSLAEAVRRADASKTTARVGAGTTEAAGLTRGRLGHPPDREVVLVKVAAEDGT
                     PIAALWNYAIHGTTLGARNRKLSGDVMGLASAEIERALGVPALFVNGAVGDVSPARHG
                     LDEARGAGRALARAVLGAWPRTPASRRGPLEVASTRVSLPAPRLSLRNCLGRFLPRGL
                     SLPLDSAFPRDAELHAGRLGDVAWVTIPGELQSQLGEAVKRNAGAGRTFLAGVTNDYL
                     GYFVTSGDYDTVAYVTCASLYGPAAGERLVDAAGDLLRALPGAER"
     gene            complement(3644..3904)
                     /gene="rpsT"
                     /locus_tag="VLF19_00395"
     CDS             complement(3644..3904)
                     /gene="rpsT"
                     /locus_tag="VLF19_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSE91737.1"
                     /translation="MANTKSAKKRMRQSEKRRVRNRMVRSKVRTAVKTARTAVASSAA
                     DASAAVLDAIRVLDKAVTRGVIHRNTAARKKSALARGLKSAS"
     gene            3988..>4789
                     /locus_tag="VLF19_00400"
     CDS             3988..>4789
                     /locus_tag="VLF19_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HSE91738.1"
                     /translation="MTTERPVLAALGPIGVATLASRVLGFGREMVVALAFGAGPVTDA
                     FFVAFRIPNVLRRLLAEGALSTAVVPVFADYAASRPREDFLRMARAVLGGALLALTVT
                     TLVGIASAPWLLRAVAPGLASEGGQASLAVLLTRVMFPYLLLVGVSALAVGALNTHGR
                     FFAAALGPAVLNVGMIAGVLFLAPRSEPPVLGLAIGVLAGGLGQLLVQVPSLHVSGLL
                     VGPSAELGHPALGRVLKLLVPAVFGLAAVQVMVFVNTQLASLLPAGSIS"
BASE COUNT          521 a         1758 c         1803 g          707 t
ORIGIN      
        1 ggcgccgaca ccgcgcggct cttctcgctc ttcgcggcgc cgccggagaa ggacctcgac
       61 tggaacgacc acggtgtcga gggggcgttc cggttcctga accgcgtgtg gcggttcgtg
      121 accgcccatc tggacgagct cgcgcccggg cccgccgtcg cggcggcagc cacgctttcg
      181 cccgacggca gggccttccg gcggacgatc cacgagacga tccggcgggt caccggcgac
      241 atcgagcgcg acttccattt caacacggcg gtgagcgcca tcatggagct ggtcaacgcg
      301 ctccaggcgt tcgaggcgtc gacgctcgac cggctgccgg ccggcgagcg tcgggcgctg
      361 atgtgcgagg ccgtggacac gctggtgctc ctgctggcgc ccttctgccc gcacgtcacc
      421 gaggagctct gggagcggct gggccgggcc ggcggggtgg tcgcgcagcg ctggccggag
      481 gccgatccag cggcgctggt gaaggacgag gtcaccgtcg tcgtccaggt cgacggcaag
      541 gtgcggagcc gtctcaccgt ggacgtgggc gcgtcggagg ccgaggtgcg gcgccaggcg
      601 ctggaggacg acaaggtgcg gccctggctc ggggcccgca cgatcgaccg cgtcgtcgtg
      661 gtgccgaacc ggctcgtgaa catcgtgacg cgctcgtgac ccgccacggg gcgttccccg
      721 ccgtcgtgct ggtcctgctg ggcgcgctgg ccggggggtg tggatactct ctccgcggca
      781 ccctgccgga ccacctcaag accatcgccg tgcccgtctt cgccaaccgg acgcgcgagc
      841 cgttcgtcga gaacatcgtc acccgcgctg tcgtcgaggc gttcacgacc agtggccggc
      901 tgcgcgtggt ccggccggag gaggcggact cgatcctcga gggcgaggtg accggctacg
      961 acctgcagtc ggtggccttc gactcggccg cgaacgtccg gcaattccgc ctggtggtga
     1021 cgatgaacct ccggttccgt gacctgcgcg agaacaaggt gctctacgat cgcgccggcg
     1081 tgcaggagcg cgcggacttc cgcgttcccg cctcggtgtc ggacacgatc gcgcgcgagc
     1141 agtcggcgct ccaggtcgcc gcggtcgaga tcgggcgggc cgtggtgagc ttcaccatcg
     1201 agcggttctg atcccgtccc cggggcccgg cctggacgac gcggcctttc tccgcagcct
     1261 cgagcgcggt cccgtgccgc cgatcgtgct cgtgcatggc gccgacgcgc agtcgctgga
     1321 cgacacgctg gccgcggtca cccgcgcgct gttcaccaac ccgtcgtcga cggccttcga
     1381 tcgcgaggtc ttcgacggcc gggagaccga cccggaggcc atcgtcaccg cggccctcac
     1441 gctcccgctg gccgccccgc ggcggctcgt ggcggtgcgc cgcgcccagg cggtgccggc
     1501 acgcgccgcc gaggcgctgg cacgctacgt ggccgcgccc aacccctccg cctgccttct
     1561 gctgctcgcc gacgaggcgc tgcacccctc gcgcgaccgc aggaccgcgc actggctcct
     1621 gggcgtggtc ccgccggcgg cggtcgtcgc gccggcgctg cggcggggcc gggcggtcga
     1681 ggagtggctg cgcgcgcggg tccaggtcga gggcctcacg attggcgctg acgccgcgcg
     1741 gctcctcgtg cagtgggtcg gggacgacgg cgccaccctg ctggcggagg cgcgcaaggc
     1801 cgcgctggcg ggcgggcccg acaactgcgc ggtgggcgtc aaggaggtcg aggccgtcgt
     1861 cggcgagcac aaggtcagcg gcgtcttcga tctcacgcgc gccatcgagc ggcgcgaggt
     1921 cggtctggcg ctgcgcacgc tggagcgcct gctcgcgacg gaagacccga tgccggtgct
     1981 ggtcatgctc acgcgcgagg tgcgcacggc gtggagcgtg cgcgagtggc acgccgccgg
     2041 acagtcggtc gagcagatgg cccggcgcct cggccggccg ccggcggtcg tccaggccgt
     2101 cgtcaccacg gcggcggggc agtcgccggc ggcgctcgcc cgcaagctcg ccctctgctg
     2161 gcacgccgag cgccggctga agtcgggcgg ggaggcacgc gccgagatga cggcgctggt
     2221 cgcggaactc gcgcgccgtg gctagggctt gcggcgccgc aggaatcctc gccgtcctgc
     2281 tcctcgcgac acgggtgtcg gcggcggacg cctgtcccgc gtgcctgcgc gctggcgccg
     2341 cctccgtcgc gctgcgcgtg ccggcgggcg ccccgctggc cggctacggc agcctcagcc
     2401 gccgcctgct cctccccgac gtgctcggcc gtcacgccca cgccttctgg ttcaagcctc
     2461 acgacggcgc cctcgacgag gtgcgggcgc gcgccctcgt cgtcgagacg gcgaccgtcc
     2521 gcgtggtgtg ggtcacggcg gacctcatcg ccgtcggccg cgagttcacc gcgcggctgg
     2581 cgcgccggct cggcgagatc ggcgtgccgc cagggaggct gctgttgtcg gcctcgcaca
     2641 cgcactcggg gccgggcgcg ttcatcgact cggcggtgat gggcttcatc tccctggacc
     2701 gcgtcgacga gggcgtccgc gaggcgctca tcggaagcct cgccgaggcg gtgcgccgcg
     2761 ccgacgcgtc caagacgacg gcccgcgtgg gcgcggggac gacggaggcc gcggggctca
     2821 cgaggggccg gctgggccac ccgcccgatc gcgaggtcgt cctcgtgaag gtcgccgccg
     2881 aggacgggac gccgatcgcc gcactctgga actacgccat ccacggcacg acgctcggcg
     2941 cccgcaatcg gaaactgtcc ggcgacgtga tggggctcgc ctcggcagag atcgagcgag
     3001 cgctcggggt gcccgcgctc ttcgtgaacg gcgccgtcgg tgacgtgagc cccgcgcgcc
     3061 acgggctcga cgaggcgcgc ggcgcgggtc gcgcgctggc gcgggcggtg ctcggcgcct
     3121 ggccgcggac gccggcgtcg cggcgggggc cgctcgaggt cgcgagcact cgggtgagcc
     3181 tgccagcacc gaggctctcg ctgcgcaact gtcttggtcg cttcctgccg cgcggcctct
     3241 cgctgccgct cgactccgcc ttcccgcgcg acgccgagct acacgcgggc cgcctcggcg
     3301 acgtggcgtg ggtgaccatt ccgggcgagc tgcagtcgca gctgggcgag gcggtcaagc
     3361 gcaacgccgg cgccgggcgg acgttcctgg ccggggtcac caacgactat ctcggctact
     3421 tcgtgacgtc cggcgactac gacaccgtgg cctacgtgac gtgcgccagc ctgtacggac
     3481 ccgccgccgg agaacgactg gtggacgcgg ccggcgacct gctgcgggcg ctgccgggag
     3541 cagagcggtg acgcgacggg atgccgggtc aacgaggcgc gccacggcct tgccggggcg
     3601 tgccgagcct gggggtcttg ggggccatct cggggccccc gtgtcacgag gcggacttca
     3661 ggccacgggc gagcgcggac ttcttgcggg cagccgtgtt gcggtggatc acaccccggg
     3721 tgacggcctt gtccagcacc cggatcgcgt cgagcaccgc cgcactcgcg tcggcggcgc
     3781 tcgacgcgac ggcggtccgg gcggtcttga ccgccgtgcg gaccttggag cggaccatgc
     3841 ggttgcgcac gcggcgcttc tcgctctgcc gcatccgctt cttcgccgat ttcgtgttcg
     3901 ccaacggccg gtgcctcctg ggctggagtt cgagacccgc gcagcattct gcccgaacgg
     3961 tccgggaatt gccagcggct tgacggcgtg acgacggagc gcccggtcct cgccgccctc
     4021 ggcccaatcg gtgtcgcgac gctggcgagc cgggtgctcg gcttcggccg cgagatggtg
     4081 gtggcgctcg ccttcggcgc cgggccggtc accgacgcct tcttcgtcgc gttccggatc
     4141 ccgaacgtgc tgcgccgcct gctcgcggag ggcgcgctct ccacggccgt cgtgccggtg
     4201 ttcgccgact acgcggcgag ccggccgcgc gaggactttc tccgcatggc gcgcgccgtc
     4261 ctcggcgggg cgctgctcgc gctcaccgtg acgacgctcg tcggcatcgc gtcggcgccg
     4321 tggctgctcc gtgcggtcgc cccggggctc gcttccgagg gtgggcaggc gtcgctcgcc
     4381 gtgttgctga cgcgcgtgat gttcccgtat ctcctgctcg tcggcgtctc cgcgctcgcc
     4441 gtgggcgcgc tcaacacgca cggacgcttc ttcgccgcgg cgctcggccc cgccgtcctc
     4501 aacgtcggca tgatcgccgg ggtgctgttc ctggccccgc gttcggagcc acccgtgctc
     4561 ggcctcgcca tcggcgtgct cgcaggcgga ctcggccagc tgctcgtcca ggtgccgagc
     4621 ctgcacgtca gcggcctcct cgtcggcccc tcggccgagc tcgggcatcc cgcgctcggc
     4681 cgcgtcctga aactgctggt gccggctgtc ttcgggctgg ccgcggtgca ggtgatggtc
     4741 ttcgtcaaca cgcagctggc ctcgctgctg cccgccggca gcatctcgt
//
LOCUS       DATIAZ010000014         5958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1318508, whole genome shotgun sequence.
ACCESSION   DATIAZ010000014 DATIAZ010000000
VERSION     DATIAZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318508"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="VLF19_00405"
     CDS             complement(<1..1649)
                     /locus_tag="VLF19_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HSE91739.1"
                     /translation="MALTGLGTGHAARLATTLAVAVLYVLAGKLGLSFASLHASASPV
                     WPPAGIALAAFLLLGYHVWPAVFAGAFLVSITTAGTVATSAGIALGNTLEALAVAWLV
                     NRFAGGAAVFDRVQGILKFLGLAGLASTTLGATIGVTSLALGGQAPWADYRFIWLTWW
                     LGDAAGLLIVAPLLVLWLRTPVPIELRQRPVEAVLLLASLVITGLVVFAGVLPPLQRR
                     PIEYLGIPPLLWAALRFGPAVTSAATVVLCGIAVAGTLQGTGPFARGTANESLLLLQG
                     FMATIAVTMLAVAVLVWDRRRADDAIRIHEARLRVAVDAARMGTWEWTIHTGAVEWSP
                     SLEAMHGLAPGSFAGTYEAFQADIHPDDRAGVDRAVLDALERGEHRVEYRIVRPDGAV
                     RWVEGRGRVFRDAEGRPERMIGVCLDVTERRAAEEERARTLERLGFLGEIARSIGSSL
                     DLDTVLQRIAEGATALCRSDSAVIFLRDDPSDAMVPRYRVGPWLRVYDTLRVRPGEGL
                     GGEVMRTGRPVRVERYQDDARVPRDFHHIAAETGTVALMVVP"
     gene            1723..4047
                     /locus_tag="VLF19_00410"
     CDS             1723..4047
                     /locus_tag="VLF19_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE91740.1"
                     /translation="MGEFGIGQAIKRFEDPRLLRGEGRYLGDVTLPGQTYMVVVRSPH
                     AHARIRSIDTAAARSAPGVLAVFTGADVARDGLGTMRMTLKRRRPDGSPMFARPHPGL
                     VHDRVRYVGDPVAVVIAETPAQAKDAAERVVVDYEPLPSVTATAEAGPGSAPVWDECA
                     DNVSNVFEAGDRGATDAAFARAHRVIRRRYVITRVHAQFMEPRGAMGVYDPGEDRYTL
                     YADVQYPHRVRNALATNIFRVPEHKLRVIAGDIGGAFGTKGWQYAEHRLVLWAARKLR
                     RPVRWACERSEAILADEHARDNISEAELALDAAGRFLGLRVRTVANIGAYLSSDRNLL
                     STFANVGTLTGVYAFPAAHVQVTCVFTNASATAPYRGAGRPEAIYVVERLIDDTAREL
                     GVDAVELRRQNLVPASAMPYRNAFGQTYDSGAFEKSMDEALKLADVDGFAARREAARQ
                     RGMRRGLGVANAIEKAAGPTLEYAEIRFDASGTATVLMGTKNQGQGHETTFRQIVHSR
                     LGLDPADVRYIDGDTDRVAFGTGTMGSRSTVIGGTALWMAADKIIAKGKKIAARLLEA
                     AEADIVCADGRFTVTGTDRGVTMREVARAAFQPASLPKGLEPGLYESGTFQPPGDTYP
                     NGCHVCEVEVDEATGEVCLVNYVVVDDVGTVINPVTLKGQIHGGLAQGLGQAIMEQVV
                     YDRESGQVLTASFMDYAIPRADTFCDITVEGNPVPTKLNPLGAKGAGEAGTVGALPAV
                     MNAVLDALAPLGVRELDMPATSERVWRAIQTARA"
     gene            complement(4142..5026)
                     /locus_tag="VLF19_00415"
                     /pseudo
     CDS             complement(4142..5026)
                     /locus_tag="VLF19_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
     gene            complement(5031..>5958)
                     /locus_tag="VLF19_00420"
     CDS             complement(5031..>5958)
                     /locus_tag="VLF19_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE91741.1"
                     /translation="PMKVKFGMPTTPPNLVHITPWVALEQGFFKAEGLDVEISTFEGG
                     LYVTRNVVSGALDAGGAVGATTAVSVARKAGIRAIYGMAPRFASTMTVRADVKTLQDL
                     KGKKVGIQEVGGFADILSRMALRKAGLKPEDVTFVPIASADVPPLLAGAIDTAILHVD
                     QLVTARRKEPSLHPLVKFWELEPNQLFQVVLAQDKKLQAEPAKYQALVRALAKANRFM
                     YANKAKTVEVAVKHAKLAADIAAEAYDELARGKVWSQNDGLPRAKVEYTIDRMVQVGN
                     IKPAERPTYEDYVAVQIVQEALKGLPRQADYD"
BASE COUNT          858 a         2123 c         2171 g          806 t
ORIGIN      
        1 ggcaccacca tcaacgcgac ggtgcccgtc tcggcggcga tgtggtggaa gtcccgggga
       61 acgcgcgcgt cgtcctggta gcgctccact cgcaccggcc ggcccgtccg catcacctcg
      121 ccgcccagac cctcgccagg cctgacccgc agcgtgtcgt agacgcgcag ccaggggccg
      181 actcggtagc gggggaccat ggcgtcggac gggtcgtcgc gcaggaagat cacggccgag
      241 tcgctgcggc agagagccgt ggcgccctcg gcgatccgct ggagcaccgt gtcgaggtcc
      301 agcgacgagc cgatcgagcg ggcgatctcg ccgagaaagc ccaggcgctc cagggtcctc
      361 gcacgctcct cttccgccgc ccggcgctcc gtcacgtcca ggcagacgcc gatcatgcgc
      421 tcgggccggc cctcggcgtc gcggaagacc ctgccgcggc cctcgaccca ccgcacggcg
      481 ccgtcgggtc tcacgatgcg gtactcgacg cggtgctcgc cgcgctcgag ggcgtcgagg
      541 acggcgcggt ccacgccggc acgatcatcg ggatggatgt cggcctgaaa ggcctcgtac
      601 gtgccggcga agctgccggg cgcgagtccg tgcatcgcct cgaggctcgg agaccactcg
      661 acggccccgg tgtggatcgt ccactcccac gtccccatgc gcgcggcgtc gaccgccacg
      721 cggagccgcg cctcgtgtat gcggatggcg tcgtccgcgc gccggcggtc ccacaccagg
      781 acggcaacgg ccagcatggt cacggcgatg gtggccatga atccctggag cagcagcagc
      841 gactcgttcg cggtgccgcg tgcgaagggg cccgtgccct ggagcgtccc ggccaccgcg
      901 atgccgcaca ggacgacggt ggcggcactc gtgacggccg ggccgaagcg cagcgccgcc
      961 cacaagagcg gcgggatgcc gagatactcg atcggacgcc gctggagcgg gggtagcacg
     1021 ccggcaaaga cgacgagccc ggtgatgacc agcgacgcca ggagcaggac ggcctcgacg
     1081 ggccgctggc gcagctcgat cggcacgggc gtgcgcagcc agaggaccag caacggggcg
     1141 acgatgagca ggccggccgc gtcgcccagc caccacgtca gccagatgaa ccggtagtcg
     1201 gcccacggcg cctgaccgcc gagggcgagg ctggtgaccc cgatggtcgc gccgagtgtc
     1261 gtgctggcca ggccggcgag gccgaggaac ttgaggatgc cctggacgcg gtcgaagacg
     1321 gccgcgccgc cggcgaagcg gttcacgagc caggcgacgg ctagcgcctc gagcgtgttg
     1381 ccgagggcga tgccggcgga cgtggctacg gtgcccgccg tcgtgatgct cacgaggaag
     1441 gcgccggcga acaccgccgg ccacacgtgg tagcccagca gcaggaacgc cgccagcgcg
     1501 atgcccgccg ggggccagac gggcgaggca ctggcgtgga gggatgcgaa ggacagcccg
     1561 agcttgcccg ccagcacgta gaggacggcg acggcgaggg tcgtcgccag gcgggcggcg
     1621 tgtccggtcc cgagtcctgt cagggccatc gtcgggcgct ctggggcgcg tggagcgcta
     1681 tcgtaacctg agttaccctt ggcgcggagg tcgggacgaa gaatgggaga gttcgggatc
     1741 ggacaggcga tcaagcggtt cgaggacccg cggctgctgc ggggggaggg gcggtacctg
     1801 ggcgacgtga ccctccccgg ccagacgtac atggtcgtcg tgcgctcgcc gcacgcccac
     1861 gcgcggatcc ggtccatcga cacggcggcc gcgcggtcgg cgcccggcgt gctggccgtc
     1921 ttcacgggcg ccgacgtcgc gcgcgacggc ctgggcacca tgcgcatgac gctcaagcgc
     1981 cgccgcccgg acggctcgcc gatgttcgcg cggccgcacc cgggtctggt ccacgaccgc
     2041 gtccggtacg tcggcgaccc ggttgccgtc gtgatcgccg agacgccggc gcaggccaag
     2101 gacgccgccg agcgcgtggt cgtcgactac gagccgttgc cgtcggtcac cgcgacggcc
     2161 gaggccggcc ccgggagcgc gccggtctgg gacgagtgcg ccgacaacgt ctcgaacgtg
     2221 ttcgaggcgg gcgatcgcgg ggccaccgac gccgccttcg cccgggccca ccgcgtgatc
     2281 cgccgccgct acgtgatcac ccgggtgcac gcgcagttca tggagccgcg cggcgcgatg
     2341 ggcgtgtacg accccggcga ggaccgctac acgctctacg ccgacgtgca gtacccgcac
     2401 cgcgtgcgca atgccctggc gacgaacatc ttccgcgtgc cggagcacaa gctccgcgtc
     2461 atcgccggcg acatcggcgg cgccttcggc accaagggct ggcagtacgc cgagcaccgc
     2521 ctcgtgctct gggcggcccg gaagctgcgc cggcccgtgc gctgggcgtg cgagcgaagc
     2581 gaggcgatcc tggccgacga gcacgcgcgc gacaacatca gcgaggccga gctggccctc
     2641 gacgcggccg gccgcttcct gggcttgcgc gtgcgcaccg tggccaacat cggcgcctac
     2701 ctgtcctcgg accggaatct gctctcgacc ttcgccaacg tcggcacgct caccggcgtc
     2761 tacgccttcc ccgccgccca cgtgcaggtc acctgcgtgt tcaccaacgc cagcgcgacg
     2821 gcgccgtacc gcggtgccgg ccggccggag gccatctacg tcgtcgagcg gctcatcgac
     2881 gataccgccc gcgagctggg cgtggacgcc gtggagctcc gccggcagaa cctcgtgccg
     2941 gcctcggcga tgccctatcg gaacgccttc ggccagacgt acgacagtgg cgcgttcgag
     3001 aagagcatgg acgaggccct caagctcgcg gacgtggacg gcttcgccgc gcggcgcgag
     3061 gccgctcgcc agcgcggcat gcggcggggc ctcggcgtcg ccaacgccat cgagaaggcg
     3121 gcgggcccca cgctcgagta cgccgagatc cggttcgacg cgagcggcac ggcgaccgtg
     3181 ctcatgggca ccaagaacca gggccaggga cacgagacca cgttcaggca gatcgtccac
     3241 tcccgcctcg gcctcgaccc cgcggacgtc cggtacatcg acggcgacac cgaccgcgtg
     3301 gccttcggca ccggcaccat gggctcgcgc tcgacggtga tcggcggcac cgcgctgtgg
     3361 atggcggccg acaagatcat cgcgaagggc aagaagatcg cggcgcgcct cctggaggcc
     3421 gccgaggccg acatcgtctg tgccgacggc cggttcacgg tgacgggcac cgaccgtggg
     3481 gtgacgatgc gtgaggtcgc ccgcgccgcg ttccagcccg cgagcctgcc gaaggggctc
     3541 gagcccggtc tctacgagtc gggcacgttc cagcccccgg gcgacacgta cccgaacggc
     3601 tgccacgtgt gcgaggtcga ggtcgacgag gcgaccggcg aggtgtgcct cgtcaactac
     3661 gtcgtggtgg acgacgtcgg caccgtgatc aacccggtca cgctcaaggg gcagatccac
     3721 ggtggcctcg cgcaggggct cgggcaggcg atcatggagc aggtcgtcta cgatcgcgag
     3781 tccggccagg tgctgacggc gtcgttcatg gactacgcga tcccgcgcgc cgacacgttc
     3841 tgcgacatca cggtcgaggg caatcccgtg ccgacgaagc tgaacccgct cggtgccaag
     3901 ggtgccggcg aggccggcac ggtcggggcg ctgcccgccg tcatgaacgc cgtcctcgac
     3961 gcgctggccc cgctcggcgt ccgcgagctg gacatgccgg ccacgagcga gcgcgtctgg
     4021 cgcgccatcc agaccgcgcg cgcgtagtcg cgaccgtccg gcgtccggtc ctgccttcgc
     4081 gcggcggcga gcggggtagg cacggaggtg ggggccgaag cgccatgttg cgcggcgaga
     4141 ttcacgctgg acgaaccagt ccgatgtctc cgaagtagcg cagggcgccg gggtggatgg
     4201 cctcgagtcg cggcgcggcg gcgacggtgt tcgcagcggt cgtctccctg gcctgcgcga
     4261 ggcgctttcc cagcgcggcc tcgccgcgat ggagcccacg ggcgagccga tagaccacgt
     4321 cctcggcgag gtcgggcctg gccaggatga ggctccagga gccgaccgac acgatgggcg
     4381 tgtcctggcc cgggtaggag ccggccgggg cggtcagggt cttgaggaag ccgtgcttgt
     4441 cctggatacg cgtgatgccc ttcgcgtccg gggcgatgaa ccgggcgccg gcgggcccgc
     4501 gcgcgacggc gacgaagccc ggccatccga ggccgccgcc ccagagcgcg gcgacccggc
     4561 cgtccatcac catcttcggc ccgtcgcccg cctggtcgag gaacacggcc tggaagtcgc
     4621 ggtgcatgtc gagtccgagc ccgtcgagca cgtagcggac gagcaccacg agccccgagc
     4681 ccttcgcgcc gaaggcgaca ggcttgcccc ggaggtcctc gatcgtccgg tgcggcgcgt
     4741 cggcgcggac cacgaacatg cccggcgtcg agtacatcgc gctcaggatg cgcagactcg
     4801 ccgggggccg cccgatgccg gccatggcct cgtgggtgac ctcgccggtg gcgagcccga
     4861 gatcgatctg cccggcctcg agcaatggca cgttctcggt actgcccttg gtgttccggg
     4921 tctggaccgc cagtgtcggc tcggtctcgt tgatggtctc ggccacggcc tgaccgtaga
     4981 ccggaaatcc gccgccgggc gtcgccgttc ccagcaccac cggcgtcttc tcagtcatag
     5041 tcggcctggc ggggcagtcc cttcaaggcc tcctggacga tctgcaccgc gacgtagtcc
     5101 tcgtacgtcg ggcgctccgc gggcttgatg ttgccgacct gcaccatccg gtcgatcgtg
     5161 tactcgactt ttgcgcgcgg cagcccgtcg ttctgcgacc agaccttgcc gcgggccagc
     5221 tcgtcgtacg cctcggcggc gatgtcggcc gcgagcttcg cgtgcttcac cgcgacctcg
     5281 accgtcttcg ccttgttcgc gtacatgaac cgattcgcct tggcgagcgc gcgcacgagc
     5341 gcctggtact tggccggctc cgcctggagc ttcttgtcct gggccaggac gacctggaag
     5401 agctggttcg gctcgagctc ccagaacttc acgagcgggt ggagcgacgg ctccttccgg
     5461 cgcgccgtga cgagctggtc gacgtggagg atggcggtgt cgatggcgcc ggccagcagg
     5521 ggcggcacgt cggcagacgc gatggggacg aacgtcacgt cctcgggctt gaggcccgcc
     5581 ttgcgcagcg ccatccggct caggatgtcg gcgaacccgc cgacctcctg gatgccgacc
     5641 ttcttgccct tgaggtcctg gagcgtcttg acgtcggcgc gcacggtcat ggtcgacgcg
     5701 aagcgcgggg ccatgccgta gatggcccgg atgccggcct tgcgcgccac cgacaccgcc
     5761 gtcgtcgcgc ccaccgcgcc gccggcgtcg agggctccgc tgacgacgtt gcgggtcacg
     5821 tagaggccgc cctcgaacgt ggagatctcc acgtccaggc cttccgcctt gaagaagccc
     5881 tgctccaggg ccacccacgg cgtgatgtgg acgaggttcg gcggcgtcgt cggcatgccg
     5941 aacttgacct tcatcggc
//
LOCUS       DATIAZ010000015        18903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1581647, whole genome shotgun sequence.
ACCESSION   DATIAZ010000015 DATIAZ010000000
VERSION     DATIAZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 18903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18903
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581647"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /locus_tag="VLF19_00425"
     CDS             complement(<1..1211)
                     /locus_tag="VLF19_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSE91742.1"
                     /translation="MARPDYETHEHDVIVIGAGGAGLRAAIEAAAQGVSVGLICKSLL
                     GKAHTVMAEGGIAAAMGNVWPEDNWQVHFRDTMRGGKMLNNWRMAQLHAQEAPDRVLE
                     LERYGALFDRTKDGLILQRDFGGHRYARLAHVGDRTGLELIRTLQYHAIHQGIDVYME
                     CKIERLLKDGDRVAGAFGYWRNTGKFVLFKAKAVVLATGGIGKAWKITSNSWEYTGDG
                     HSLALWAGADLIDMEFVQFHPTGMVWPPSVRGILVTEGVRGDGGTLKNNKGERFMFNY
                     IPEFFKGETADNEAEADRWYEDKSNRRTPDLLPRDEVARAINSEVKAGRGSPHGGVFL
                     DICTRRPADYIKRRLPSMYHQFKELAGVDITKEPMEVGPTCHYVMGGVRVEADTTQAT
                     VPGLFAAGEVAG"
     gene            complement(1222..2055)
                     /locus_tag="VLF19_00430"
     CDS             complement(1222..2055)
                     /locus_tag="VLF19_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91743.1"
                     /translation="MIREILKDQRGHVVGAARPPIAAPHLRRDAWWALPVTVVVVLGA
                     FVVYSTWAALQNAHYYAAPYLSPFYSPCLSKSCLHPTFGWAMPDVQLPWFGIVSPAFL
                     ILWGPGLFRLTCYYYRKAYYRSFWLAPAACAVRDVKRGYTGETRVPLVLQNFHRYAWY
                     VAVIFIVLLTWDAILAFWFPAPGGGVQFGIGVGTLVMWINVILLAGYTFSCHSCRHVC
                     GGHVDMFSRAKTRYAVWHFITRLNERHPMFAWLSLFSVGLADLYIRLVSMGLIRDLRI
                     V"
     gene            complement(2052..2630)
                     /locus_tag="VLF19_00435"
     CDS             complement(2052..2630)
                     /locus_tag="VLF19_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HSE91744.1"
                     /translation="MVSAGADGIKDVTTPLSDADAESLRAGDRVRISGVIYTARDAAH
                     GRLFPLIEQGKPLPIDVRGAIVYYTGPSPARPGDVVGSIGPTTASRMDRFTPALLRLG
                     LKGTIGKGYRGQAVKDALRQYKGVYFGAIGGAGAVLSRHVKRLEVVAYEDLGTEAIRR
                     LEVEDFPAIVINDCHGGDLYQDGQKQFAREGA"
     gene            complement(2623..3177)
                     /locus_tag="VLF19_00440"
     CDS             complement(2623..3177)
                     /locus_tag="VLF19_00440"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HSE91745.1"
                     /translation="MTPGHGVTAIFRALELPADVAGRVYLASMPGRYERLEAALHEMD
                     RHGIERVVCLTPDTEIEAKAPEYLDAVRGGGLRRPCERLDVTDYRAPADENAFRVLVD
                     HIARRLRQGERILVHCAGGIGRTGTLAICLLVALGWTVQQSREAARRAGSRPESDEQN
                     AFIGGFAGSALGGITLASLDGRHG"
     gene            complement(3180..4022)
                     /locus_tag="VLF19_00445"
     CDS             complement(3180..4022)
                     /locus_tag="VLF19_00445"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HSE91746.1"
                     /translation="MRDIHVSAIADGVKKLCMEANWNLEPDMLRAFDRALQTERSAAG
                     KQVLQILKDNAELARTKRIPYCQDTGMVVCFVELGQEVHVTGGGLVDAINEGVRQGYT
                     EGYLRASIVRSPFDRVNTGDNTPAVIHVDVVPGAALHIMIMAKGGGCENRSKYRMLTP
                     AEGVAGVKDFIIECVKTAGPDACPPLVLGVGVGGTFEKSAILSKKALFRELGSPNADP
                     TLDALEKKLLEQANRLGIGPQGYGGDTTAFGIHILSYPCHITSLPVAVTIECHAHRHK
                     EVTL"
     gene            complement(4026..4505)
                     /locus_tag="VLF19_00450"
     CDS             complement(4026..4505)
                     /locus_tag="VLF19_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE91747.1"
                     /translation="MATLVPEGYHTVTPYLTIRGAADAIEFYKRAFGAQETERMPGPD
                     GKGIMHAEIRIGDSVVMLADEMPSMGCRAPQTLGGTTSYLFLYVTDVDAAFKRAVDAG
                     AKATMPPTDMFWGDRFGKLTDPFGHEWGLATHKEDVPPEEMKRRAAEAMKQLSAGKK"
     gene            complement(4579..5544)
                     /gene="mdh"
                     /locus_tag="VLF19_00455"
     CDS             complement(4579..5544)
                     /gene="mdh"
                     /locus_tag="VLF19_00455"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSE91748.1"
                     /translation="MQVGHAEPLTVVNRRPRITVVGAGNVGATVAQYVVEKELGDVVL
                     VDVIDGIPQGKALDLAQAGPVHGYDSTLVGSNGYDETANSDLVVITAGLARKPGMTRD
                     DLLFKNAEIVGSVVEQVTRRSPDAILILVTNPLDAMVQLAWRKSGFRPERVVGMAGVL
                     DSARFRTFIARELDVSVENVTAFVLGGHGDSMVPLPRYSTVAGIPITELLPPDKIDAL
                     VKRTANGGAEIVNYLKSGSAYYAPAASAVEMVEAILKDKKKILPCAAYLNGQYGVKGL
                     YVGVPVKLGRNGVEQVIEITLSADEQAAFQRSVAAVRELVDKLKL"
     gene            complement(5555..6817)
                     /gene="icd"
                     /locus_tag="VLF19_00460"
     CDS             complement(5555..6817)
                     /gene="icd"
                     /locus_tag="VLF19_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HSE91749.1"
                     /translation="MAGKVKVPAGEKITIDHGKLRVPDHPVIAFIEGDGTGPDIWKAS
                     VRVLDAAVEQAYKGRKKIAWMEVHAGDKAQTVYGKDCPPNLLPPETLDYIREYLVAIK
                     GPLTTPVGEGFRSLNVTLRQEMDLYVCLRPVRWFKGVPSPVKRPDKVDMVIFRENTED
                     IYAGIEWEQGTPEAKKVVDFLQTQMGVKKIRFPNTSSIGIKPVSKEGSERLIRAAIRY
                     ALENNRRNVTFVHKGNIQKYTEGMFMKWGYALAKREFADRTIGWEECGGKPPAGKMLL
                     KDAITDAFLQQILTRPDEFDVIPCCNLTGDLISDALAAQVGGIGIAPGANINYDTGHA
                     LFEATHGTAPKYAGQDKVNPGSVILSGEMMLRYMGWTDAADRIIAGLEKTIQEKVVTY
                     DLARLMEGAKEVKCSEFGTAIVQSMARL"
     gene            complement(6817..9156)
                     /locus_tag="VLF19_00465"
     CDS             complement(6817..9156)
                     /locus_tag="VLF19_00465"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HSE91750.1"
                     /translation="MSAAQIRSLYSAKPDRLARARNVFGRPLTLAEKILVAHCWSFDD
                     QQWERGKAILRLRVDHVALQDATGQMALLQFMMSGRKRVAVPTTQHCDHLIRAESGAA
                     DDFKRAITENKEVYDFLRSASKKFGLGFWRPGSGIIHQVLLENYAFPGGLMIGADSHT
                     PNCGGLGMIAIGVGGADCAEAMAGLPWEVLHPRLIGVRLTGKLAGWTAPKDVITYLCT
                     RLTVKGGTNKILEYFGPGAESISATGKGTICNMGAELGATTSLFPFDRRMTDYLRATE
                     RADIAAAAEEFRDHLVADREVLADPGKYYDEIIEVDLDRLEPHVVGPHSPDRGRPISQ
                     LASEAKQNGWPLTITNALIGSCTNSSYEDMRRAAHVATQAIKAGLRAKVPFYITPGSD
                     RIFQTIKRDGIIDTFQRIDGTVLANACGPCIGQWKRRDVQVDQTNTIVSSFNRNFPGR
                     NDGNNATLSFISSPEIVTALAFAGTLEFDPVHGTLTGPDGKQFRFTPPEAEDLPRDGF
                     ARGLEGYEAPAEDGDAVRIELPENSERLQLLGPFVAWDGKDLIDLPLLVKTKGKTTTD
                     HISPAGAWLRYRGHLDKISDNMFLGAVNAFTGETGKGVNPLTGAAGQPFAKTARELKA
                     RGQPWVVVGDENYGEGSSREHAAMSPRYLGCAAVIVRSFARIAETNLKKQGILPLTFA
                     DPKDYDRVEQDDRVSIVGLYGLAPGAPVTVRLTKKDGRVVEIRGNHTLTKEQIAWFRA
                     GSALNAGQAPVDPATGRAAQLEDVKLRPGDVDVKPGGSQ"
     gene            9329..9595
                     /locus_tag="VLF19_00470"
     CDS             9329..9595
                     /locus_tag="VLF19_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91751.1"
                     /translation="MTPRVALLMVLVGALLVMAVQPPPAEADPTVAIALASAAVIVLI
                     LVAYLIVANVDEQRRTLRDGQDMPTLVVYAPPAPGADGGRYDAP"
     gene            complement(9580..10302)
                     /locus_tag="VLF19_00475"
     CDS             complement(9580..10302)
                     /locus_tag="VLF19_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HSE91752.1"
                     /translation="MATLRVFRWSSGQRERWQDYDVAAGTDTTVLDALVAVQRGPDPT
                     LAFRYACRVGMCGSCAVVVNGRERWACRTRLATLGAGPVTVRPLYHFPLLRDLVVDMA
                     PFHRRLRDVGAFAPRADAEPYALVPRDSAERREIDPSIECIGCGMCVSACTISAWNAR
                     FPGPAALNRAFTLQRDSRDGATAGRWAGLLSEDALPRCHGQGNCTEVCPMGLSPAGSI
                     VRLRQMAARKLLRGWSRAQGAS"
     gene            complement(10328..11194)
                     /locus_tag="VLF19_00480"
     CDS             complement(10328..11194)
                     /locus_tag="VLF19_00480"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HSE91753.1"
                     /translation="MKRVKVLDSEMAYVDTGRGTPVVFLHGNPTSSYLWRNVIPHVGE
                     MSCPQTDPVARCLAPDLIGMGRSGKAPAGSYRFADHARYLDAWFETLGLSDVTLVVHD
                     WGSALGFDWARRHPHRVRGLVYMEAIVRPVTSDEWPENARKIFQAMRSPSGEEIVLQK
                     NVFVERILPGSIMRRLSDDEMAEYRRPWLEPGEVRRPMLTWPREIPMDGQPADVVAIV
                     EDYARWLATSPIPKLFVNAEPGSILVGAQRESCRRWPNQREVTVRGLHFVQEDSPGEI
                     GRAVADFVRSLG"
     gene            complement(11253..11570)
                     /locus_tag="VLF19_00485"
     CDS             complement(11253..11570)
                     /locus_tag="VLF19_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91754.1"
                     /translation="MTRILRREWTTVEGWRDTKAGMWAWLLQRAAAVLLLIVIALHLV
                     NPFRRGVQAALLALALLHALLGVRSLLLDVGVPLRWHRALFALALALAAVLFVLVWAW
                     RWY"
     gene            complement(11567..12754)
                     /locus_tag="VLF19_00490"
     CDS             complement(11567..12754)
                     /locus_tag="VLF19_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE91755.1"
                     /translation="MDRFVLTGATLVDGTDAPPGRGKAVVVEQGRIAAVVDEGQAPAG
                     RVLRLDGLTLLPGLVNAHVHLCLGAEPDPARTLREDPLGLTAIKAVVRARQTVEAGVT
                     TVRDLSGREYVELAVRDAVRDGLVVGPRILAAGRGICMTGGHGWWFGREADGPVEARR
                     AVREQLKAGADVIKIFATGGVMTAGADPNAPQLEADEIRAAVDEARKAGRRTAAHAQA
                     TQGIAWCVEAGISSIEHGVFLTEPLVRRMARDGVALVPTLIAPHAIAAGGATAGIPEF
                     MVQKSEALRGRHVESFQLALAAGVTIAAGTDAGTPLNPHGSMVPELTLMVKAGMAPAA
                     AVRAATSVAARLLGLEHETGRVAPGLAADLLAVAGDPTEDVRALEQVRLVVVRGRIVV
                     ERP"
     gene            13126..14613
                     /locus_tag="VLF19_00495"
                     /pseudo
     CDS             13126..14613
                     /locus_tag="VLF19_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
     gene            14851..15027
                     /locus_tag="VLF19_00500"
     CDS             14851..15027
                     /locus_tag="VLF19_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91756.1"
                     /translation="MKRWEGAIAEMVKDPAFLAVTDRLWMHLDHLTAAEMSVFVEKQA
                     AYYTEMAAKIGIRR"
     gene            complement(15119..16855)
                     /locus_tag="VLF19_00505"
     CDS             complement(15119..16855)
                     /locus_tag="VLF19_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSE91757.1"
                     /translation="MTPAPDVREVDVLILGAGGAGLMAALHAAEAAPSLRVAVVVKGL
                     LGRAGCTRMVQGGYNAVLTPPDSLEAHLADTLAGGGFINDQELVWTLVREAPGRVLEL
                     ESRYGCLFDRTAEGRIHQKPFAGQTHDRTVHKGDLTGIEIMNRLSEQTLARANIEAFE
                     ECRAVDLLLDDHGRSAGALVLDVRTGGFLSIRARATLLAMGGGPTMYKVIACSADKAA
                     DGIALAWRAGLPLRDMEMIQFHPTGLVIPGSLMTGALLEEGLRGAGGRLLNGRGERFM
                     ARHDPARMERSTRDLVSRASFLEVAEGRGTPNGGVWIDVSHLGAAVVERNFRGMVRRC
                     RDFGRDLAREPVEVAPTAHFMMGGVVIDPACQTSIEGLFAAGEDTGGVHGANRLGGNG
                     VAESTVFGGIAGDVMAEFVAGRRPPVVSTAGLRETVDALTAPLGRPAGGDLYGLLAAL
                     RETMWERAGLVRDGAGLREAIVEAGRVGTALEHVGVSGGPALNLAWQDWLNVKSLAAV
                     ARLVALSALERSESRGAHFRRDFPAAESGPLYSVRVQRVGVETTARRDSVVLTRVTPT
                     PQPVPATVEIGD"
     gene            complement(16852..17154)
                     /locus_tag="VLF19_00510"
                     /pseudo
     CDS             complement(16852..17154)
                     /locus_tag="VLF19_00510"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            complement(17167..17430)
                     /locus_tag="VLF19_00515"
     CDS             complement(17167..17430)
                     /locus_tag="VLF19_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91758.1"
                     /translation="MSRRIAAVATGLVLVATLTVLWLATRPASTGRAGAGGVALDDLM
                     RDLGIVPVTGAAKPFALPALDGRKVALADFKGRPVLVYFWASW"
     gene            complement(17461..18381)
                     /locus_tag="VLF19_00520"
     CDS             complement(17461..18381)
                     /locus_tag="VLF19_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE91759.1"
                     /translation="MRLTVMEPFRSLFYAPQFVALHLGHFADEGFDVTVRTGADATGT
                     TRALIDGTAQFSLGGIMRSLDLADRGGRFLVHFAEVNSRNGFFLLAREARPGFTWPDL
                     AGRTVISFAGAPTPWQCMLTVLRRHGVDPARVTFVRDLHGDDAVRAFREGRGDFLETG
                     QPTAEALIAEGAAHLVVSMGDATGPVPFSSYMTTPERLRREPELCERFTRAVYRAQRW
                     IAANDGRAIAGVVAPSFPDVPGELAARAVERYRRQGTWAADPICRQPGYEYLVDILLA
                     GGFIRRRHRYADLVDTEIARRVVATTRPGP"
     gene            complement(18462..18794)
                     /locus_tag="VLF19_00525"
     CDS             complement(18462..18794)
                     /locus_tag="VLF19_00525"
                     /inference="COORDINATES: protein motif:HMM:NF020350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HSE91760.1"
                     /translation="MSDIGKVRIRVPSSIKAGDVVRVRTLVIHPMERVERDPQGKVVQ
                     KKYRYIDRVVATYLGKTVAAFDTTQSVAENPSFAFTFRATDPGQLKVVFHDTHGATWE
                     GTADIRLS"
     gene            complement(18802..18888)
                     /locus_tag="VLF19_00530"
     CDS             complement(18802..18888)
                     /locus_tag="VLF19_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91761.1"
                     /translation="MRAIVEQSDGTLLQVKREVKVTVGGCGG"
BASE COUNT         2681 a         6748 c         6548 g         2926 t
ORIGIN      
        1 cccgcgacct caccggccgc gaagagcccg ggcacggtcg cctgtgtcgt gtcggcttcc
       61 acccgcaccc cgcccatcac gtagtgacag gtcgggccga cctccatcgg ctccttcgtg
      121 atgtcgaccc cggccagctc cttgaactgg tggtacatgg acggtaaccg cctcttgatg
      181 tagtcggccg gacggcgggt gcagatgtcg aggaacacgc caccgtgcgg gctgccgcgg
      241 ccggccttga cctcggagtt gatggcgcgc gccacctcgt cgcgcggcag caggtcgggc
      301 gtgcggcggt tgctcttgtc ctcgtaccag cggtcggcct cggcctcgtt gtcggcggtc
      361 tcccccttga agaactcggg gatgtaattg aacatgaacc gctcgccctt gttgttcttg
      421 agcgtcccgc cgtcaccccg cacgccctcg gtcacgagaa tgccgcgcac ggagggcggc
      481 cacaccatgc cggtcgggtg gaactggacg aactccatgt cgatgaggtc cgcgcccgcc
      541 cacagggcga gcgagtggcc gtcgcccgtg tactcccagg agttcgacgt gatcttccac
      601 gccttgccga tgccgccggt ggcgagcacc acggccttgg ccttgaagag cacgaacttg
      661 cccgtgttcc gccagtagcc gaaagcgccg gcgacgcggt cgccgtcctt gagcagccgc
      721 tcgatcttgc actccatgta gacgtcgatc ccctggtgga tcgcgtggta ctggagggtg
      781 cggatcagct cgagcccggt gcgatcgccc acgtgggcca gtcgcgcgta gcggtggccg
      841 ccgaagtcgc gctggaggat gagcccgtcc ttggtgcggt cgaagagcgc cccgtagcgc
      901 tccagctcga gcacgcggtc cggcgcctcc tgggcgtgca gctgcgccat gcgccagttg
      961 ttgagcatct tgccgccgcg catggtgtcg cggaagtgca cctgccagtt gtcctcgggc
     1021 cacacgttcc ccatggccgc ggcgatgccg ccctcggcca tgaccgtgtg ggccttgccc
     1081 aggagcgact tgcagatcag ccccacggag acgccctgcg ccgccgcctc gatggccgcg
     1141 cgcaggccgg cgcccccggc gccgatcacg atgacgtcgt gctcgtgtgt ctcgtagtcc
     1201 ggccgtgcca tcggtccccc tctagacgat ccggagatcg cggatcaggc ccatcgacac
     1261 caggcggatg tagaggtccg ccaggcccac gctgaacagg ctcagccacg cgaacatcgg
     1321 atgccgctcg ttcagacggg tgatgaaatg ccagacggcg tagcgcgtct tcgcccgcga
     1381 gaacatgtcc acgtggccgc cacagacgtg gcggcaggag tggcaggaaa acgtgtaacc
     1441 cgccagcagg atcacgttga tccacatcac cagcgtgccg acgccgatac cgaactgcac
     1501 gccgccgccc ggcgcgggga accaaaacgc gaggatcgcg tcccaggtga ggaggacgat
     1561 gaagatcacc gcgacgtacc acgcgtaccg gtggaagttc tgcaggacga gaggcacgcg
     1621 cgtctcgccc gtgtagcccc gcttcacgtc gcgcacggcg caggccgccg gcgccagcca
     1681 gaacgagcgg tagtacgcct tgcggtagta gtagcaggtc agccggaaca ggcccggccc
     1741 ccagaggatc aggaacgccg gcgacacgat gccgaaccac ggcagctgga cgtcgggcat
     1801 cgcccagccg aacgtcgggt gcaggcacga cttcgaaagg cagggggagt agaacggcga
     1861 gaggtacggc gccgcgtagt agtgcgcgtt ctggagcgcc gcccacgtcg agtacacgac
     1921 gaaggcgccg agcaccacga ccaccgtgac gggcagcgcc caccacgcgt cccggcgcag
     1981 gtgcggcgcc gcgatgggcg gtcgcgccgc gccgacgacg tggccccgct ggtccttcag
     2041 gatctcgcgg atcatgcgcc ctcccgcgcg aactgcttct ggccgtcctg gtagagatca
     2101 ccgccgtggc agtcgttgat gacgatggcc ggaaagtcct cgacctcgag ccgccggatg
     2161 gcctcggtgc ccaggtcctc gtaggcgacg acctcgagcc tcttgacgtg ccggctcagc
     2221 acggcgccgg cgccgccgat ggcgccgaag tacacgccct tgtattgcct gagcgcgtcc
     2281 ttcacggcct ggccgcgata gcccttgccg atcgtgccct tgagcccaag ccggagcagg
     2341 gccggcgtga acctgtccat ccggctcgcc gtggtcggcc cgatggatcc gacgacatct
     2401 cccggccggg ccggcgaggg tcccgtgtag tagacgatgg cgcccctgac gtcgatcggc
     2461 agcggcttgc cctgctcgat cagcgggaac agacggccgt gagcggcatc gcgcgccgta
     2521 tagatcacgc cggagatgcg cacgcggtcg ccggccctca gcgactccgc gtcggcatcg
     2581 ctcagcggcg tcgtgacgtc cttgatcccg tccgctcctg cgctaaccat ggcgaccgtc
     2641 cagcgaggcc agtgtgatgc cgccgagggc gctgccggcg aagcccccga tgaacgcgtt
     2701 ctgctcgtcg ctctccggcc tcgaccccgc ccgccgggcc gcttcccggc tctgctgcac
     2761 cgtccagccg agcgcgacca gcaggcagat cgccagcgtc ccggtccggc cgataccgcc
     2821 ggcgcagtgc acgaggattc gctcgccctg acgcagtcgc cgggcgatgt gatccacgag
     2881 aacacgaaac gcgttctcgt ccgccggcgc acggtagtcg gtgacgtcga gccgctcgca
     2941 gggtcgccgc aggccgcccc ctctcaccgc gtccaggtac tccggcgcct tggcctcgat
     3001 ctcggtgtcc ggagtcagac acacgacccg ctcgatcccg tgccggtcca tctcgtggag
     3061 ggccgcctcc aggcgctcgt accggcccgg catgctcgcg agatagacgc ggcctgcgac
     3121 gtcggccggc agctccagcg cgcggaagat cgccgtcaca ccatgtcccg gggtcatggc
     3181 tagagggtga cttccttgtg gcggtgcgcg tggcactcga tcgtcacggc cacgggcagc
     3241 gaggtgatat ggcatggata gctcaggatg tggatcccga aggcggtggt atcaccgccg
     3301 tagccctgcg gcccgatgcc gagccggttg gcctgctcga gcagcttctt ctccagcgcg
     3361 tcgagcgtcg gatcggcgtt cggggagccg agctcgcgga acagtgcctt cttgctgagg
     3421 atggcggact tctcgaaggt gccgcccacg ccaacgccga ggacgagcgg cgggcaggcg
     3481 tcgggccccg ccgtcttcac gcactcgatg atgaagtcct tcacccccgc cacgccctcg
     3541 gccggcgtga gcatcctgta cttcgaacgg ttctcgcagc cgccgccctt ggccatgatc
     3601 atgatgtgga gcgccgcccc cggcaccacg tcgacgtgga tcaccgccgg cgtgttgtcc
     3661 ccggtgttca cgcggtcgaa cggcgaccgg acgatcgagg cgcgcaggta gccttccgtg
     3721 tagccctgcc gcacgccctc gttgatcgcg tcgacgaggc ccccgccggt cacgtggacc
     3781 tcctgcccca gctcgacgaa gcagacgacc atgccggtgt cctggcagta ggggatccgc
     3841 ttcgtccgcg ccagctccgc gttgtccttg aggatctgga gcacctgctt gccggcggcc
     3901 gaccgctcgg tctggagcgc ccggtcgaag gcgcgcagca tgtcgggctc caggttccag
     3961 ttcgcctcca tgcagagctt cttgacgccg tcggcgatgg cgctgacgtg gatgtcgcgc
     4021 atcgcctact tcttccccgc gctcagctgc ttcatcgcct cggccgcccg ccgcttcatc
     4081 tcctcgggcg gcacgtcttc cttgtgcgtg gccaggcccc actcgtgtcc gaaggggtcg
     4141 gtcagcttgc cgaagcgatc cccccagaac atgtcggtcg gcggcatcgt cgccttcgcg
     4201 ccggcgtcca cggcgcgctt gaaggccgca tcgacgtccg tcacgtacag gaacaggtag
     4261 ctggtcgtgc cgccgagcgt ctgcggggcg cggcacccca tggagggcat ctcgtccgcg
     4321 agcatcacga ccgagtcgcc gatacggatc tcggcgtgca tgatgccctt gccgtccggc
     4381 cccggcatgc gctccgtctc ctgggccccg aacgcacgct tgtagaactc gatcgcgtcc
     4441 gccgcgcccc ggatcgtcag gtagggagtg acggtgtggt atccctcggg aaccagcgtg
     4501 gccatggtcc ttcctccacg ggatggcgat ccgggagctc tccggtgacg gagcgccccc
     4561 ggcatcgtcg tcccgccgct acagcttcag cttgtcgacc agctcgcgca ccgccgccac
     4621 cgagcgctgg aacgccgcct gctcgtccgc gctcagcgtg atctcgatga cctgctccac
     4681 gccgttccgc ccgagcttca cggggacacc gacgtagagg cccttcacgc cgtactggcc
     4741 gttcaggtag gcggcgcacg gcaggatctt cttcttgtcc ttcaagatcg cctcgaccat
     4801 ctcgacggcc gaggcggccg gagcgtagta ggcgctcccg gacttgaggt agttgacgat
     4861 ctccgcgccg ccgttggccg tgcgcttgac gagcgcgtcg atcttgtcgg gcggcagcag
     4921 ctcggtgatg gggatgccgg ccaccgtgga gtagcgcggc aacggcacca tgctgtcgcc
     4981 gtggccgccc agcacgaacg cggtcacgtt ctcgaccgac acgtccagct cccgcgcgat
     5041 gaacgtgcgg aaccgcgccg agtccagcac gccggccatg cccacgacgc gctcgggtcg
     5101 gaagccggac ttccgccacg cgagctgcac catggcgtcg agggggttcg tcacgagaat
     5161 gaggatcgcg tctggcgagc gccgcgtgac ctgctcgacg accgagccga cgatctcggc
     5221 gttcttgaac agcaggtcgt cgcgcgtcat gcccggcttg cgcgcgaggc cggcggtgat
     5281 gaccacgagg tcggagttcg ccgtctcgtc gtagccgttg gagccgacca gcgtcgagtc
     5341 gtagccgtgg acagggccgg cctgggcgag gtcgagggcc ttgccctgag ggatgccgtc
     5401 gatcacgtcc acgagcacga cgtcgcccag ctccttctcg acgacgtact gcgcgacggt
     5461 ggcgcccacg ttaccggcgc cgaccacggt gatccgggga cggcggttga ccacggtcag
     5521 cggctcggcg tgcccgactt gcatgcccgt tctcctacag cctggccatg ctctggacga
     5581 tcgccgtccc gaactccgag cacttcacct ccttcgcgcc ctccatgagc cgcgcgaggt
     5641 cgtaggtgac gaccttttcc tggatggtct tctcgaggcc ggcgatgatc cggtccgcgg
     5701 cgtcggtcca gcccatgtag cggagcatca tctcgccgga gaggatgacc gagccggggt
     5761 tgaccttgtc ctggccggcg tacttcggcg cggtgccgtg ggtggcctcg aagagggcgt
     5821 ggccggtgtc gtagttgatg ttggcgccgg gcgcgatgcc gatgcccccc acctgcgcgg
     5881 ccagcgcgtc ggagatcagg tcgccggtga ggttgcagca cgggatcacg tcgaactcgt
     5941 ccggccgggt gaggatctgc tgcaggaacg cgtcggtgat ggcgtccttg agcagcatct
     6001 tgcccgcggg cggcttgccg ccgcactcct cccacccgat cgtgcggtcg gcgaactccc
     6061 gcttggccag cgcgtagccc cacttcatga acatgccttc ggtgtacttc tggatgttgc
     6121 ccttgtgcac gaacgtcacg ttgcgccggt tgttctcgag cgcgtagcgg atggccgcgc
     6181 ggatgaggcg ctcggagccc tccttcgaca cgggcttgat gccgatcgac gacgtgttcg
     6241 gaaagcggat cttcttgacg cccatctgcg tctggaggaa gtccacgacc ttcttcgcct
     6301 ccggcgtccc ctgctcccac tcgatgccgg cgtagatgtc ttccgtgttc tcgcggaaga
     6361 tcaccatgtc gaccttgtcc ggccgcttca ccggcgacgg cacacccttg aaccaccgca
     6421 ccgggcgcag gcagacgtac aggtccatct cctggcgaag cgtcacgttg agcgaccgga
     6481 agccctcgcc gacgggggtg gtgagcggcc ccttgatggc gacgaggtac tcgcggatgt
     6541 aatcgagcgt ctccggtggc agcaggttcg gcgggcagtc cttgccgtag acggtctggg
     6601 ccttgtcgcc ggcgtggacc tccatccacg cgatcttctt tcgccccttg taggcctgct
     6661 cgaccgcggc gtcgaggacg cgcacgctgg ccttccagat gtcgggaccc gtgccgtcgc
     6721 cctcgatgaa ggcgatgacg gggtggtcgg gcacgcgcag cttgccgtgg tcgatggtga
     6781 tcttctcgcc tgcggggacc ttgaccttgc ctgccattac tgactgcctc ctggcttgac
     6841 gtcgacgtcg ccgggacgca gcttcacgtc ctcgagctgt gccgcgcgtc cggtagccgg
     6901 atccacgggc gcctgaccgg cgttgagcgc cgagcccgcc cggaaccagg cgatctgctc
     6961 cttggtgagg gtgtggttgc cgcggatctc cacgacccgg ccgtccttct tcgtgaggcg
     7021 caccgtgacc ggcgcgcccg gcgcgagccc gtagaggccg acgatgctca cgcggtcgtc
     7081 ctgctcgacg cggtcgtagt ccttcgggtc ggcgaacgtc agcggcagga tgccctgctt
     7141 cttgaggttc gtctcggcga tgcgcgcgaa cgatcgcacg atgaccgcgg cgcagccgag
     7201 atagcgcggc gacatcgcgg cgtgctcacg gctggagccc tcgccgtagt tctcgtcgcc
     7261 gaccacgacc cacggctggc cccgcgcctt cagctcgcgc gccgtcttcg cgaacggctg
     7321 cccggccgcg ccggtgagcg ggttcacccc cttgcccgtc tcgccggtga aggcgttgac
     7381 cgcgccgagg aacatgttgt cgctgatctt gtcgaggtgg ccgcggtacc gcagccaggc
     7441 accggccggg gagatgtggt cggtggtggt cttgcccttg gtcttgacga ggagcggcag
     7501 gtcgatgagg tccttgccgt cccaggcgac gaacggcccc aggagctgca gccgctcgct
     7561 gttctccggc agctcgatcc gcacggcgtc gccgtcctcg gcgggggcct cgtaaccctc
     7621 cagcccacgc gcgaagccgt cgcgcggcag gtcctcggcc tccggcggcg tgaaccggaa
     7681 ctgcttgccg tcgggccccg tcagcgtccc gtgcacggga tcgaactcca gcgtgccggc
     7741 gaaggccagg gcggtgacga tctccgggct cgagatgaac gacagcgtcg cgttgttgcc
     7801 gtcgttgcgc cccgggaagt tgcggttgaa ggacgacacg atcgtgttcg tctggtccac
     7861 ctgcacgtcg cggcgcttcc actggccgat gcacgggccg cacgcattgg ccagcacggt
     7921 gccgtcgatg cgctggaacg tgtcgatgat gccgtcgcgc ttgatggtct ggaagatgcg
     7981 gtcggagccc ggcgtgatgt agaagggcac cttcgcgcgc agtccggcct tgatggcctg
     8041 cgtcgccacg tgggccgcgc gccgcatgtc ctcgtacgag gagttcgtgc aggagccgat
     8101 cagcgcgttg gtgatggtca gcggccagcc gttctgcttc gcctcgctgg cgagctggct
     8161 gatcggccgg ccgcggtcgg gggagtgcgg gcccaccacg tggggctcga gccggtcgag
     8221 atcgacctcg atgatctcgt cgtagtactt cccggggtcc gccagcacct cgcggtcggc
     8281 gaccaggtga tcgcggaact cctcggcggc agcggcgatg tccgcgcgct ccgtggcgcg
     8341 cagatagtcg gtcatgcggc gatcgaaggg gaagagcgac gtggtggcgc cgagctcggc
     8401 gcccatgttg cagatggtac ccttgccggt ggcgctgatc gactcggcgc cgggcccgaa
     8461 gtactcgagg atcttgttgg tgccgccctt gacggtcagg cgcgtgcaga gataggtgat
     8521 cacgtccttc ggcgcggtcc agccggcgag cttgccggtg agccgcacgc cgatgagcct
     8581 ggggtgcagc acctcccacg gcaacccggc catcgcctcg gcgcagtcgg cgccaccgac
     8641 gccgatcgcg atcatcccca ggccaccgca gttgggcgtg tgggaatcgg cgccgatcat
     8701 gagcccgccg gggaaggcgt agttctcgag gaggacctgg tggatgatgc cggagcccgg
     8761 gcgccagaat ccgagaccga acttcttcga ggccgaccgc aggaagtcgt agacctcctt
     8821 gttctccgtg atcgcgcgct tgaagtcgtc ggcggcgccc gactcggcgc ggatcaggtg
     8881 gtcgcagtgc tgggtcgtgg ggacggcgac ccgcttgcgc cccgacatca tgaactgcag
     8941 cagcgccatc tggcccgtcg cgtcctgcag cgccacgtgg tccacccgca gtcgcaggat
     9001 cgccttgccc cgctcccact gctggtcgtc gaagctccag cagtgggcga cgaggatctt
     9061 ctcggcgagc gtcagcggac gaccaaagac gttgcgggcc cgggcgaggc ggtcgggttt
     9121 cgcggagtag agtgagcgga tctgcgcggc ggacatccag ggcctccctc gtcgagtacg
     9181 acgctgggca aacgttcgcc tcccgtccgt cgcggacggg aggcgcgcaa acgacggata
     9241 actctaacgc cacgccgggt tagggtcaag atacgcaccg cgccgattct tttctcggat
     9301 cgcggcctcg ctctgctagc gtgcgggcat gacaccacgc gtggccctcc tcatggtcct
     9361 cgtcggcgcc ctgctcgtca tggccgttca acccccgccg gccgaggcgg acccgaccgt
     9421 cgcgatcgcc ctcgccagcg ctgccgtcat cgtcctcatc ctcgtcgcct atctgatcgt
     9481 cgccaacgtc gacgagcagc gacgaaccct gcgggacggg caggacatgc ccacgctcgt
     9541 agtgtacgcg ccgccggcgc ccggagccga cggcgggcgc tacgacgcgc cctgagcgcg
     9601 cgaccacccc cgcagcagct tcctcgccgc catctgcctc aggcgcacga tcgatccggc
     9661 cggcgacagg cccatcgggc acacctccgt gcagttcccc tggccgtggc agcgcggcag
     9721 cgcgtcctcc gagagcagcc ccgcccagcg tcccgccgtg gccccgtcgc gcgagtcgcg
     9781 ctgcagcgtg aacgcgcggt tcagcgccgc gggcccgggg aagcgcgcat tccaggcgct
     9841 gatcgtgcac gccgagacgc acatgccgca gccgatgcac tcgatcgacg gatcgatctc
     9901 ccggcgctcg gcggagtcgc gcgggacgag ggcgtagggc tcggcgtcgg cccggggtgc
     9961 gaaggcgccg acgtcgcgga ggcgccggtg gaacggcgcc atgtccacca ccaggtcgcg
    10021 caggagcgga aagtggtaga gcgggcgcac ggtgaccggg ccggcgccca gcgtggcgag
    10081 ccgcgtgcgg caggcccagc gctcgcgccc gttcacgacg acggcgcagg acccgcacat
    10141 cccgacgcgg caggcgtagc ggaaggcgag cgtcggatcc ggcccgcgct gcaccgccac
    10201 gagcgcgtcg aggacggtcg tgtcggtgcc ggccgccacg tcgtagtcct gccagcgctc
    10261 gcgctggccc gacgaccagc ggaagacgcg gagcgtcgct atccccgcac ctccgcggag
    10321 gtcggcctca cccgagactc ctcacgaagt ccgccacggc gcggccgatc tcgcccggcg
    10381 aatcctcctg gacgaagtgc agccctcgca cggtgacctc ccgctggttc ggccaccgcc
    10441 ggcaggactc gcgctgcgcg ccgaccagga tggatcccgg ctccgcgttg acgaagagct
    10501 tggggatcgg gctcgtggcg agccaccggg cgtagtcctc tacgatggcc acgacgtcgg
    10561 ccggctggcc gtccatgggg atctcgcgcg gccacgtgag catgggccgg cgcacctcgc
    10621 cgggctcgag ccacggccgg cggtactcgg ccatctcgtc gtcgctgagc ctgcgcatga
    10681 tgctcccggg caggatccgc tcgacgaaga cgttcttctg gagaacgatc tcctcgccgg
    10741 agggtgagcg catggcctgg aagatcttgc gcgcgttctc gggccactcg tccgacgtca
    10801 ccgggcgcac gatcgcctcc atgtagacga ggccccgcac gcgatggggg tgcctccgcg
    10861 cccagtcgaa gccgagtgcc gagccccagt cgtgcaccac gagggtcacg tcggagagcc
    10921 cgagcgtctc gaaccaggcg tcgagatacc gcgcgtggtc cgcgaagcgg tacgagccgg
    10981 ccggcgcctt gcccgagcgg cccatgccga tcaggtccgg cgccaggcac cgcgcgacgg
    11041 gatcggtctg cgggcaggac atctctccga cgtgcgggat cacgttcctc cacaggtacg
    11101 acgacgtcgg gttgccgtgc aggaacacca ccggcgtccc gcggcccgtg tcgacgtagg
    11161 ccatctcgct gtcgagcacc tttacgcgct tcatgaggtc accggggtcc ggccatcgat
    11221 tgtaacgcgc ctgacggagg ccagcgcgcg gttcagtacc agcgccaggc ccagacgagc
    11281 acgaacagca cggccgccag cgccagggca agggcgaaga gggcgcgatg ccagcgcagc
    11341 ggcacgccga cgtccagcaa cagcgagcgg actcccagga gcgcgtggag cagcgccagc
    11401 gccagcagcg ccgcctgcac gccccggcgg aaggggttga cgaggtgcag cgcgatcacg
    11461 atcagcaaca gcacggcggc ggcgcgctgc aggagccacg cccacatgcc ggccttcgtg
    11521 tcgcgccacc cctcgaccgt ggtccactcg cggcggagga tcctggtcac gggcgttcaa
    11581 cgacgatccg gccgcgcacg acgacgagcc gcacctgctc cagcgcccgg acgtcctccg
    11641 tggggtcgcc ggcaacggcg aggaggtcgg cggcgagccc gggagcgacg cgcccggttt
    11701 cgtgctcgag accgagcagt cgcgcggcga ccgaggtggc ggcgcggacg gcggcggccg
    11761 gcgccatccc cgccttcacc atcagcgtga gttcggggac catggagccg tgggggttga
    11821 gcggcgtgcc ggcgtcggtg ccggcggcaa tggtcacccc cgcggcgagg gcgagctgga
    11881 agctctccac gtgccggccg cgcagggcct ccgacttctg gaccatgaac tccgggatcc
    11941 cggcggtcgc gccaccggcg gcgatcgcgt gcggggcgat cagcgtcggc acgagcgcca
    12001 caccgtcgcg cgccatgcgc cgcacgagcg gctcggtgag gaacacgccg tgctcgatgg
    12061 agctgatccc ggcctccacg caccaggcga tgccctgcgt ggcctgcgcg tgggcggcgg
    12121 tgcgacgccc ggccttgcgc gcctcgtcca cggccgcgcg gatctcgtcg gcctcgagct
    12181 ggggcgcgtt gggatcggcg ccggccgtca tcacgccgcc ggtcgcgaag atcttgatga
    12241 cgtccgcgcc ggccttgagc tgctcgcgca cggcgcgccg cgcctccacg gggccatccg
    12301 cctcgcgccc gaaccaccag ccgtgaccgc ccgtcatgca gatgccgcgc cccgcggcca
    12361 gaatgcgcgg gccgacgacg agcccgtcgc gcaccgcgtc gcgcaccgcc agctccacgt
    12421 actcccggcc gctgaggtca cgcaccgtcg tgacaccggc ctcgacggtc tggcgcgcgc
    12481 gcacgacggc cttgatcgcg gtgagcccga gcgggtcctc gcgcagcgtt cgggccgggt
    12541 ccggctcggc gccgaggcag aggtggacgt gcgcgttgac gagcccgggc aggagcgtga
    12601 gtccgtcgag tcggaggacc cgccccgccg gcgcctgccc ttcgtccacc acggcggcga
    12661 tccgcccctg ctcgaccacg acggccttgc cacggcccgg cggcgcgtcg gtgccgtcga
    12721 ccagcgtcgc gcccgtcagc acgaatcgat ccatctcacc gttctccgcg aatgtgcctc
    12781 gacagcgcgc ggcactccgg cggcaagtcg tcgagcgacg cctcacacgt cggcgatcac
    12841 tctgtgcgga tcggcctccg ccaacagttg cgcgatgtcc cacgcgtcct gggtccgccc
    12901 gcgaacagct tcaggaggac aaggtcagcc acgtccgcca cgggtaccgc ggcggcctcg
    12961 atcggcgatg caacctctcc cctcgccgcc ctgccagcgc cgaactgccg ccacctcgcc
    13021 gagcaggctc agccgatgac ctccgagaga caccggcagg ggcaacgccg ttgccactcc
    13081 tcgctcctca acccgtcggc gacgcttcgc ctgccccccg gcaatatagc aggtggcgcc
    13141 ccgaggctcc cgtccctgtc cacgcgggca gcatttgtgg gcgcgctgct cgggacgctc
    13201 gtgggtatcc tccccgggct tggccccacc gccggcatcg ccattctgtt ccctctgacc
    13261 gccgccctcc cacccacggc cgccatcatc atgctcggcg cgatctgcga tggcgcgatg
    13321 tacgggggct cgacgaccgc gatcctcctg aacatccccg gagaggtggc atccgtgccg
    13381 acgacgtggg acggttatcc cctggcccag caggggcggg gcgggtctgc cctcggcgtg
    13441 gccgcgatcg ggtcgttctt cgccggaacc gttggcgtcg tcgcactcac gttctttgcg
    13501 ccaatcctgg ccgaccatgc gctgcgattc ggtccgcccg agtacttcgg cctcatggtc
    13561 cttgccttca cagtggtcgg gtcgctctcc ggtgccgccc tcgccaaggg gattgccatg
    13621 gccctggtgg gctacttcct ctccctggtc ggcatgggac ccgccaccgc ccagccgcgt
    13681 ttgaccttcg gcttccagga cctctacgcc ggagtggaaa tgatcgcgct gatcgtcggc
    13741 ctgttcgcga tctcggaagt cctgctcggg atcgaggagg cgcgggccac gatcgcggag
    13801 aggatccgca acgtgtaccc cagcctggcc gacatcaggc agagctttcc cgccatcctg
    13861 cgcggaactg ggatcggctt cctgctcggg ctcctgcccg gctgcgcccc gagcatcacg
    13921 gtctttctcg cctacgacac ggagaagaaa gtcgcgaagc acccagagcg atttggcaac
    13981 ggcgccatcg agggtatggc gtctccagag tcggccaaca acgcgaccag ctccgccggg
    14041 ttcatcccgc tcatggcctc ggcattcccg cctcggtgcc actggcgctg ctgctgggcg
    14101 ggctcatggt ctacgggctg accccgggcc cgctcctctt tcagaagaac ccggacttcg
    14161 tctggacggt catcgcgagt ctctacattg ggaacgtcat cctcctcata ctgaacctcc
    14221 cgctcgtcgg catctgggtg cgtatcacgc gcatccccta cgggatcctg gggcccgtca
    14281 tcctgctcct ctgttttctc ggggtctaca ccgtccgcac agcatgttcg acgtgggcgt
    14341 gagcctcgtc ttcgggctgg tgggctacgt gctccgcaag tatcattggc cgctcgctcc
    14401 gctgctgctg gcctacatcc tcggcccgct gctcgagaag aaccggattc aggcgctctc
    14461 catgagcggc gggagcccct gatcttcctg cagaggaggc tgtcgctatc gcttctggtg
    14521 gcggcggcag ctctggtggc cctctccctc gtcctcgtgc ggggcacagt ccgccgcgtc
    14581 gagcaggtcg aggcggaggc cggaatcgcc tgactgaaca aggaggtcga gcgatgcgat
    14641 cgtggcgatg gagcatgctc gcgtgggtga tggtcctgcc gacgtggctg gccggcgccg
    14701 gtgacgcgtt cgcgcagacg tacccgacgc gaccggtgga gatcgtggtg ccgttcgtgc
    14761 ccgggggcgg gaccgacctg atcgcatccg ctacctgccc gacgtggtgg gccggcattt
    14821 ccgttccggc cgcgaccccc cgacttcatc gtgaagcggt gggagggcgc catcgccgaa
    14881 atggtgaagg atcccgcttt cctggcggtc accgatcgtc tctggatgca cctcgaccat
    14941 ctgaccgccg ccgagatgag cgtgttcgtc gagaagcagg ccgcctacta cacggagatg
    15001 gcggcgaaga tcggcatccg ccggtaggcg cctgtctggt ccagaggcgc gccacagctg
    15061 tgctggggcg tgccgaacgc cggggggctt gggggccagc tcggggcccc catgtcagtc
    15121 aatcaccgat ctcgacggtc gcgggcaccg gctgaggcgt cggcgtcacg cgggtcaaca
    15181 ccaccgaatc gcgtcgtgcg gtggtctcga cgccgacgcg ctgcacgcgc acgctgtaga
    15241 gcggtccgct ctcggccgcg gggaagtccc ggcggaagtg cgcgccgcgg ctctcgctgc
    15301 gctcgagcgc gctcagggcc acgaggcggg cgaccgccgc caggctcttg acgttgagcc
    15361 agtcctgcca ggcgaggttc agcgccggcc cccccgacac gccgacgtgc tcgagcgccg
    15421 ttccgacgcg ccccgcttcg acgatggcct cgcgcagacc cgcgccgtcg cggacgaggc
    15481 cggcgcgctc ccacatcgtc tcgcgtagcg cggccaggag cccgtagaga tcgcctccgg
    15541 cggggcgacc gagcggcgcc gtgagcgcgt ccaccgtctc ccgcaggccg gccgtcgaga
    15601 ccaccggcgg gcgtcggccg gcgacgaact ccgccatcac gtcgcccgcg atgccgccga
    15661 acaccgtcga ctcggccacg ccattgccgc ccaggcggtt ggcgccgtgc acgccgccgg
    15721 tgtcctcgcc ggcggcgaag agcccctcga tggacgtctg gcacgccggg tcgatcacga
    15781 cgccgcccat catgaagtgc gcggtgggcg ccacctcgac gggctcgcgc gcgaggtcac
    15841 ggccgaagtc gcgacagcgc ctcaccatgc cgcggaagtt gcgctcgacc accgcggcgc
    15901 ccaggtgcga gacgtcgatc cagacgccgc cgttcggcgt gccgcggccc tcggcgacct
    15961 cgaggaacga ggcccgcgat accagatcgc gcgtggagcg ctccatgcgg gccgggtcgt
    16021 gccgcgccat gaaccgttcg ccacggccgt tcagcaggcg gccgccggcg ccgcgcagcc
    16081 cttcctccag cagcgccccc gtcatgagcg agccggggat cacgaggccg gtcgggtgga
    16141 actggatcat ctccatgtcg cgcagcggga ggccggcgcg ccaggcgaga gcgatgccat
    16201 cggcggcctt gtcggccgag caggcgatga ccttgtacat ggtcggaccg ccgcccatgg
    16261 ccagcagcgt ggcgcgcgcg cggatggaga gaaagccacc ggtgcgcacg tcgagcacga
    16321 gggcgccggc gctgcggccg tggtcatcca ggagcaggtc gacggcccgg cactcctcga
    16381 acgcctcgat gttggcgcgg gcgagcgtct gctcggacag gcggttcatg atctcgatgc
    16441 ccgtgaggtc ccccttgtgc acggtgcggt cgtgggtctg accggcgaac ggcttctggt
    16501 ggatccggcc ctcggccgtg cggtcgaaga ggcagccgta gcgcgattcc agctcgagca
    16561 cccgccccgg cgcctcgcgc acgagcgtcc acaccagctc ctggtcgttg atgaacccgc
    16621 cgccggcgag cgtgtcggcg aggtgggcct cgagcgagtc gggcggcgtg agcacggcgt
    16681 tgtagccgcc ctgcaccatc cgcgtgcacc cggcgcgccc gagcaagccc ttgacgacga
    16741 cggcgacgcg gagcgacggg gcggcctcgg cggcgtggag cgcggccatc agacccgctc
    16801 cgcccgcacc gaggatcagg acgtcgacct cgcggacgtc cggggccggc gtcacgactt
    16861 gccgagcgcc tcgagcagcg cgcggcccgt ggccgaggcc cacggtttgg tgccgaccgc
    16921 cttgccgcgg agcgtgccgt cgggcgccac gaggaacacc gtcggggtcg cgctcacgcc
    16981 gtagtcctcg gtcgcccgac cgtcggtatc gagaaggatc gtgaacgaca cgatgtgggc
    17041 gtcgacccag cgctgcacgg tctcacgccg ctcccggatg gagatggcca cgacggcgag
    17101 gccccccggc tgcgccgcct tgtagacctc ctcgatcgtc gagggcaact ccctcgtgca
    17161 gtacggtcac cagctcgccc agaaatagac gagcaccggt cgtcccttga aatcggccag
    17221 cgctaccttc cggccgtcga gcgccggcag cgcgaagggc ttcgccgccc ccgtgaccgg
    17281 cacgatgccc aggtcgcgca tgaggtcgtc gagcgcgacg cccccggccc ccgcgcgccc
    17341 ggtcgaggcg ggtcgggtcg cgagccacag cacggtgagc gtggcgacga gcacgaggcc
    17401 ggtggcgacg gcggcgatcc tccgggacat agccgcgttc atcttagcgt gattcgcggc
    17461 tcatggccct ggcctcgtgg tcgccacgac ccggcgcgcg atctccgtgt ccacgaggtc
    17521 ggcgtagcgg tggcggcgcc ggatgaagcc gccggcgagc aggatgtcca ccaggtactc
    17581 gtaaccgggc tgccggcaga tggggtccgc ggcccacgtt ccctgacgcc ggtaccgctc
    17641 caccgcccgc gccgcgagct cgccggggac gtcggggaac gagggcgcga cgactccggc
    17701 gatggcccgg ccgtcgttcg cggcgatcca gcgctgcgcg cggtagaccg cgcgcgtgaa
    17761 gcgctcgcag agctcgggct cgcgccgcag ccgctccggt gtcgtcatgt aggacgagaa
    17821 cggcacgggt ccggtggcgt cccccatcga gacgacgagg tgcgcggcgc cctcggcgat
    17881 gagggcctcc gcggtcggct ggccggtttc gaggaagtct ccgcggccct cgcggaaggc
    17941 ccgcacggcg tcgtcgccgt gcaggtcccg cacgaacgtc acgcgcgcgg ggtccacgcc
    18001 gtggcggcgg aggaccgtga gcatgcactg ccagggggtc ggcgcgccgg cgaacgagat
    18061 caccgtgcgg ccggccaggt cgggccaggt gaagccggga cgcgcctcgc gcgcgaggag
    18121 gaagaaaccg ttgcggctgt tcacctcggc gaagtgcacg aggaaccggc cgccgcggtc
    18181 ggcgaggtcg aggctgcgca tgatgccgcc gaggctgaac tgcgcggtgc cgtcgatcag
    18241 ggcgcgcgtg gtgcccgtcg cgtcggcgcc cgtgcgcacc gtgacgtcga aaccctcgtc
    18301 ggcgaagtgc ccgagatgca gggcgacgaa ctgcggcgcg tagaagagcg agcggaacgg
    18361 ctccatgacg gtgagccgca tgggggccga gtatagcagg cgccggcgga gcggacgcca
    18421 ctgcacgcgc cggccccgtc ggcgatcgtg ccgaccccgc ctcaggagag ccggatgtcg
    18481 gccgtgccct cccaggtggc cccgtgggtg tcgtggaaga cgaccttgag ctgcccgggg
    18541 tcggtcgcgc ggaaggtgaa cgcgaacgac gggttctcgg cgacgctctg cgtggtgtcg
    18601 aaggccgcga cggtcttgcc gaggtaggtg gcgacgaccc gatcgatgta gcggtacttc
    18661 ttctggacga ccttgccctg cgggtcccgc tcgacgcgct ccatcggatg gatcaccagt
    18721 gtccgcacgc gcacgacgtc gccggccttg atgctcgagg gcacccggat ccgcaccttg
    18781 ccgatgtccg acatgccgcc cctaaccgcc gcagccgccg acggtgacct tgacctcgcg
    18841 cttcacctgg agcagggtgc cgtcgctctg ctccacgatc gcgcgcacgt cgccggtctc
    18901 gcc
//
LOCUS       DATIAZ010000016        11658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_792877, whole genome shotgun sequence.
ACCESSION   DATIAZ010000016 DATIAZ010000000
VERSION     DATIAZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11658
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792877"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VLF19_00535"
     CDS             complement(<1..959)
                     /locus_tag="VLF19_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSE91762.1"
                     /translation="MPALVRPYREREALGDLGDVFAVMTDMAETCGRLTHGIARSLGA
                     SKCVVALYDPATREMVGQAPGYGVADEVIRAFRCRVDLPWLRSQGPMVKNRPSEFHPV
                     QWEYLRPFEIYNMAGVPLVVDGRIIGMVAAGNRPGGFSHRAVRSLATFAARAAIALQN
                     ARLYAHLAELAGALRVKMQNRTAELEATNRELAISHARLREVDRLKSDFLGNVSHELR
                     TPLASIKGFVDNLLDGVTGPLTSKQQHYLARMRDNADRLTRMVSDLLDLTRIEAGRLE
                     VTLARVDLVAVLNDAVESLVPLARGRGIQMAVMSASCPPVRGDA"
     gene            complement(1158..1391)
                     /gene="fdxA"
                     /locus_tag="VLF19_00540"
                     /pseudo
     CDS             complement(1158..1391)
                     /gene="fdxA"
                     /locus_tag="VLF19_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(1510..2412)
                     /locus_tag="VLF19_00545"
     CDS             complement(1510..2412)
                     /locus_tag="VLF19_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE91763.1"
                     /translation="MALTPPVDRAAHLLESPARRVWLAASVDFTRRRPLGAAGAAIVL
                     LMFVVAVTATVIAPYDPLAVDFGAMLAAPSTDHWLGTDAFGRDVLSRLIYGSRTALIV
                     GFGAAFFGATAGAILGVASAYFGGRIDLYLQRVMDIFISFPLIILALAMVAILGNNLP
                     NLITAITIPMIPRAALVIRSSALSVREMPYVDAARAAGFGHSRIIMRHMLPNVMAPYL
                     IMLTAFLGQAVLLEASLSFLGLGVQEPIPAWGLMLRGAAVQFAETAPWMAIFPGLAIS
                     LSVFAFNLFGDSLRDALDPRLRTQ"
     gene            complement(2420..3379)
                     /locus_tag="VLF19_00550"
     CDS             complement(2420..3379)
                     /locus_tag="VLF19_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE91764.1"
                     /translation="MWKYIVKRFFLMIPTLFGVAVLAFLLLRIVPGDIVEARFMGAGQ
                     GTFVSPEMIAQERAKLGLDQPLWKQFGAWIWGLARFDLGRSMWTGAPIVEEIRLRFAL
                     SLQVAVMATVFATLLAIPLGVVAALKQDTWVDYVVRIFSIAGLATPSFWLGIVIILGL
                     LIVFRWLPPMVYTPFWVDPWQNLAQLIWPALAVGYRYSAVATRMTRSAMLEVLREDYI
                     RTAKAKGLWLKLILVRHALKNAMLPVVTVIGLEFAFLLGGLVVTEQVFNLNGLGLLFV
                     EAVARRDYTLTQALVMLTASAFIVVNFIMDAMYAWLDPRIRYR"
     gene            complement(3476..5071)
                     /locus_tag="VLF19_00555"
     CDS             complement(3476..5071)
                     /locus_tag="VLF19_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE91765.1"
                     /translation="MRRLSAFLALVLIALLAVPAIAQDKPRSGGELVFPVPSEPPSYD
                     GHREETFGLTHPFAPFYNTLLRVDPTDPSGTKPVPSIAESWTVTNDGRTYTFKIRRGV
                     KFHDGSEMTSRDVKASYDKIIFPPQGIGSSRKGQYQNVEAVEAPDPSTVRFRLKYPQG
                     SFLLSIASPWNFIYKADILAKDMTWYEKNIMGTGPFTFVEHVKGSHVVGKKNPNYWDK
                     GKPYLDGFRAMFVRDSAAQVALIRGERAHIQFRGFSPKERDTLKTALGDKITVQESAW
                     DCILMVAINHEKKPFDDRRVRRALTLALDRYEGSKALSQIAIVKEVAGVQVPGTPYAT
                     PPAELASLAGYGKDINAARAEARKLLREAGVPDGFAFTFKNRGIPMPYEPLAIWMIDQ
                     WRQVGLNVKHEVVESAAYYGQVLRPGAFEVAMDFQCGYIVEPDLDLYKFISTDKNPSN
                     YGRYTDRVLDDLYEKQGRTQDQEERKKLIRAFEKRLLDEEVRYLLTLQWHRIIPHSAK
                     VKGWQITPSHYLNNTLDTVWLKE"
     gene            complement(5153..6145)
                     /locus_tag="VLF19_00560"
     CDS             complement(5153..6145)
                     /locus_tag="VLF19_00560"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HSE91766.1"
                     /translation="MTGLAPHGDVLLEVRDLVKHFAVGGGLLGRSHGIVRAVDGVSFS
                     IRRGETLGLVGESGCGKTTTGRCILQLERATSGQVIFEGRDLTTLEGAALRAVRRKVQ
                     VIFQDPYSSLNPRMTVGQIIAEPLAVHGIVPDAGSRGQRVGELLRRVGLLPQHAQRYP
                     HEMSGGQRQRVGVARALAMEPALIVCDEPVSALDVSIQAQIINLLEDLQAEFGLTYLF
                     IAHDLAVVRHISDRVAVMYLGKIAEIADRKSLYDDPLHPYTKALLAAVPIPDPELEAR
                     RERVVLRGEVPSPLDPPSGCVFHPRCPIAVDRCHEQIPPLREIRSGHWAACHLA"
     gene            complement(6142..7149)
                     /locus_tag="VLF19_00565"
     CDS             complement(6142..7149)
                     /locus_tag="VLF19_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE91767.1"
                     /translation="MAPLLEVRSLSTQFVTRAGTVRAVDDVSWDVGEGETVALVGESG
                     CGKSVSALSIMRLIQGPAGRIAGGQVLFKGRDLLTLSEEEMRKVRGREIAMVFQEPMT
                     SLNPVLTIGRQLTEGLEIHLGLSPTDARSRAVDLLAMVGIPDPQRRLPQYPHQFSGGM
                     RQRMMIAMALACEPALVLADEPTTALDVTIQAQILELMKDLSRRLGVAMLIITHNLGV
                     VARYADRVNVMYAGKIIERGTAREIYAHPRHPYTLGLLRSVPRLDEPRRERLAPIDGQ
                     PPDLARLPPGCAFAPRCAFRVDRCLAEVPALRAVPRDGHVVACWEAERLDGARTAVAA
                     R"
     gene            complement(7313..8560)
                     /locus_tag="VLF19_00570"
     CDS             complement(7313..8560)
                     /locus_tag="VLF19_00570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSE91768.1"
                     /translation="MIRVAMLSVHTCPLAALGGKETGGMNVYVRELSRELGRMGAALD
                     IFTRSQNEAIPRVVEMAETVRVVHLPAGPEAPLLRERVHEHLDEFLDGVEAWRIARGV
                     EYDLVHAHYWLSGVVALALRERWSVPVVQMFHTLGRLKNGAARSPADREPLLRLREEA
                     RLVADADLIVAASEVERAHLIGHGAEAARVTVIPCGVDTALFRPGDAGEARAALGLDD
                     GPLMLYVGRIAPIKGLDTLLDAVGRLRAHGRAPRLLIVGGDADEPRAGHEAELRRHAG
                     RLGLADVVTFVGAQPQERLRRYYVASDVTVLPSYYESFGMVALEAMACGSPVIASRVG
                     GLATTVVDGVTGFLVSDGDAGALARRLDGVLADPALRWRIGREGVRWAAQHRWPCVAE
                     AVCRAYAGLVPAAGEHLAAARCR"
     gene            complement(8557..9546)
                     /locus_tag="VLF19_00575"
     CDS             complement(8557..9546)
                     /locus_tag="VLF19_00575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE91769.1"
                     /translation="MKIALHDRLEAVGPPAWDALHRRTRLRSPFLTWTWQSEWARTFA
                     HGRRLEVWRVEDHGETVALLPLYETGPAAMELLGGADVSDYLDLVAESGREEEAWSAL
                     LAARATTPAAWDLHAVPAASATVTAVPGLAAAFGLAVTEAVEERCPVLELPASWDDYL
                     ATLSGKQRHELTRKMRRLAREVPEAGATCAATRADVEIRLDDFLGLHRRSRAGKARFM
                     DQRMEAFFRRAVTALAEVGMARLWLLDTRGGPLAAFITLEWDGVVGLYNSGFHPDHAA
                     LSPGVVLLGYLVQDAIARGRRRFDFLRGEERYKYDFGPVPEDVYRVTISPALP"
     gene            complement(9543..9926)
                     /locus_tag="VLF19_00580"
     CDS             complement(9543..9926)
                     /locus_tag="VLF19_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSE91770.1"
                     /translation="MPIYEYECRDCRRVVSLLVLRPAAAEAPRCPRCGGAALTRLMSR
                     FATVKSEEARLDALAGDASFGDIDENDPASVARFMKKMGREMGDDLGDDLDEAVDEAM
                     AGSEDDVAAPDAEATSGSGTADTEL"
     gene            10143..10337
                     /locus_tag="VLF19_00585"
     CDS             10143..10337
                     /locus_tag="VLF19_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91771.1"
                     /translation="MTEPTDLTELRGDSERLLIEAVVELARTLRDERDRLAGRLRQVE
                     SELTTLTERLEASRTAPPGA"
     gene            complement(10347..11381)
                     /locus_tag="VLF19_00590"
     CDS             complement(10347..11381)
                     /locus_tag="VLF19_00590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSE91772.1"
                     /translation="MRITFFCPHLGIAGGVRAVLTYADRLAARGHDVTVLVPVRRPWQ
                     AWWRNVHGRGPDWVPGFRPRVQWMPDFSPESVPAGDVVVATAWQTAAAVAEAPARCGA
                     KFYLIQHYESLYHGAPDRVDATYRLPLAKIVVSTWLRDVMREKFASEPEVLVTPVDTA
                     LFHPVPATVATPRPRVLMLHHDYAWKGVADGLEAVAVARRSLPSLFLVGLGVKPPRRA
                     MRYDEFHADPPQETLREVYSNCEIYLCPSWDEGLGMPPMEAMACGAALVTYDNGGCRD
                     YARDGETALVARRRDIADLATKLERLAADATLRRKIAVAGQLLVTTAFDWNRAVDRME
                     AMFVGADGRR"
     gene            complement(11386..>11658)
                     /locus_tag="VLF19_00595"
     CDS             complement(11386..>11658)
                     /locus_tag="VLF19_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSE91773.1"
                     /translation="CIIGNDGGWGQIRNPQLSFFGEERAVATSLPTTRYDLMVEALGG
                     RGVLVTDPAAIRPALERALGSDEVWCINVPLDPAAYRRTGQVSMAI"
BASE COUNT         1810 a         4055 c         4160 g         1633 t
ORIGIN      
        1 gcatcgccgc gcaccggcgg gcaggatgcg ctcatgaccg ccatctggat cccccggccg
       61 cgcgccagcg gcaccaggct ctcgacggcg tcgttgagca ccgcgacgag atcgacccgg
      121 gcgagcgtca cctcgagccg acccgcctcg atgcgcgtga gatcgagcag gtccgatacc
      181 atccgggtca ggcggtccgc gttgtcgcgc atccgcgcca ggtagtgctg ctgcttcgag
      241 gtcagcggac ccgtcacgcc atccagcagg ttgtcgacga accccttgat ggacgccagc
      301 ggagtgcgca gctcgtgcga gacgttgccg agaaaatccg acttgaggcg gtccacctcg
      361 cggagccggg cgtgcgagat ggcgagctcg cggttggtcg cctcgagctc cgcggtccgg
      421 ttctgcatct tgacgcgcag ggcgcccgcc agctccgcga ggtgcgcgta gagacgggcg
      481 ttctggaggg cgatcgcggc gcgcgccgcg aacgtcgcca gcgaccggac ggcgcggtgg
      541 gagaaccctc ccggccggtt cccggcggcg accatcccga tgatgcgccc gtcgacgacc
      601 agaggcacgc ccgccatgtt gtagatctcg aacggtctga ggtactccca ctggacagga
      661 tggaactcgg agggtcggtt cttgaccatc ggcccctggc tcctcagcca tggcaggtcg
      721 acgcggcacc ggaaggcccg gatcacctcg tcggcgacgc cgtagccggg ggcctgcccg
      781 accatctccc gcgtggccgg gtcgtacagc gcgacgacac acttgctcgc gcccagggag
      841 cgggcgatgc cgtgcgtgag ccggccgcac gtctcggcca tgtcggtcat gaccgcgaag
      901 acgtccccga ggtcgccgag cgcctcgcgc tcccggtacg ggcgcacgag agcaggcatc
      961 atcggcggcg gctcggccac cagccgtgcg ccgtcgccgg tggacgtcgg gtcagggcga
     1021 gcggcgagcg agcgcccggt cgtcccgaca aacgtcgagg ggcgccgcgc tgacacggcg
     1081 tccctcgaat cggaatgggg tcggtggcgg atccgggtcg accgctactt cttggtcgcg
     1141 ggctgcaccc cggggtggtc cttgaaaaac tgcttgttga gctcgatgaa gttcttccac
     1201 tgctccggcg tctcgtcctc cgcgaagatc gccttcaccg ggcacacagg ctcgcaggcg
     1261 ccgcagtcga tgcactcgtc ggggtggatg tagagcatgt ccggcccctc gtagatgcaa
     1321 tcgaccgggc acacttcgac gcaggccttg tccttcacgt tgatgcacgg ctcggcgatg
     1381 atatacggca tcggattccc ccttctggag tcactcaagc ctggtcggac cacgtgagtt
     1441 tttacatgac ccggcgcagc gtgtcaagca agcatatcgc agacttagcc gcgtcttgcg
     1501 gcccggcggt cactgcgtcc gcaagcgggg atcgagcgcg tcgcgcagcg agtcgccgaa
     1561 caggttgaag gcgaagaccg agaggctgat ggccaggccc gggaagatgg ccatccacgg
     1621 cgcggtctcg gcgaactgca cggcggcgcc gcggagcatg agtccccagg cgggaatggg
     1681 ctcctgcacg ccgaggccga ggaacgacag ggacgcctcg agcaggacag cctggccgag
     1741 gaacgccgtc agcatgatca ggtacggcgc catcacgttg ggcagcatgt ggcgcataat
     1801 gatgcggctg tggccgaacc cggcggcgcg cgcggcgtcc acgtagggca tctcgcgcac
     1861 gctgagggcg ctggagcgga tgacgagcgc ggcgcgcggg atcatcggaa tggtgatggc
     1921 cgtgatgagg tttggcaggt tgttgccgag gatggcgacc atcgccaggg ccaggatgat
     1981 gagcgggaac gagatgaaga tgtccatcac gcgctgcagg taaagatcga tccgccctcc
     2041 gaagtacgcg ctggccacgc cgaggatggc ccccgccgtc gccccgaaga aggccgcgcc
     2101 gaagccgacg atcagcgccg tccgcgaccc gtagatgagc cgggagagca cgtcgcggcc
     2161 gaaggcatcg gtgccgagcc agtggtccgt ggacggcgcg gcgagcatgg cgccgaagtc
     2221 cacggcgagc gggtcgtagg gcgcgatcac ggtggcggtg acggccacca cgaacatcag
     2281 gagcacgatg gccgcgccgg ccgcgccaag cgggcggcgg cgggtgaagt cgacgctggc
     2341 cgcgagccac acgcggcgcg cgggcgactc cagcaggtgc gcggcgcggt cgacgggcgg
     2401 ggtcagggcc atgggcggct cagcgatacc ggatccgcgg atcgagccag gcgtacatgg
     2461 cgtccatgat gaagttcacg acgatgaacg ccgaggcggt gagcatcacg agcgcctgcg
     2521 tgagcgtgta gtcccgccgg gccacggctt ccacgaagag caggccgagc ccgttgaggt
     2581 tgaacacctg ctcggtgacc acgagtccgc ccagcaggaa ggcgaactcg agaccgatga
     2641 cggtgaccac cggcaacatc gcgttcttga gcgcgtgccg gaccaggatc agcttcagcc
     2701 agagcccctt cgccttggcc gtccggatgt agtcctcccg cagcacctcc agcatcgccg
     2761 agcgcgtcat gcgcgtggcc acggccgagt agcggtagcc cacggcgagg gccggccaga
     2821 tgagctgggc caggttctgc cagggatcga cccagaacgg cgtgtacacc atcgggggca
     2881 gccagcggaa cacgatgagc agcccgagga tgatcacgat ccccagccag aacgagggcg
     2941 tggccagccc ggcgatcgag aagatccgca ccacgtagtc cacccacgtg tcctgcttca
     3001 gcgccgcgac cacaccgagc gggatggcca gcaaggtagc gaacaccgtg gccatcacgg
     3061 ccacctgcag cgacagggcg aaccggagcc ggatctcctc cacgatcggc gcgcccgtcc
     3121 acatggagcg gccgaggtcg aagcgggcga ggccccagat ccacgcgccg aactgcttcc
     3181 agagcggctg atccagcccc agcttggccc gctcctgcgc gatcatctcc ggcgagacga
     3241 acgtcccctg ccccgcgccc atgaagcggg cctcgacgat atcaccgggc acgatgcgca
     3301 gcagcaagaa ggccagcacc gccaccccga acagcgtcgg aatcatcaag aagaaccgct
     3361 tcacgatgta cttccacata tcccgcgctc tacgctccgt ccgcgctacc gccggaatgt
     3421 gctccgccca ccgccgtcat cgccgtgttc gagcgcgggg ttcgcccgct ccgagtcact
     3481 ccttcagcca gaccgtgtcg agcgtgttgt tcaggtagtg gctcggcgtg atctgccacc
     3541 ccttgacctt cgccgagtgc gggatgatgc gatgccactg cagcgtcaag aggtaccgca
     3601 cctcctcgtc gagcaggcgc ttctcgaagg cccggatcag cttcttgcgc tcttcttgat
     3661 cctgggtccg cccctgcttc tcgtacaggt cgtcgagcac ccgatccgtg tagcggccgt
     3721 agttcgacgg gttcttgtcg gttgagatga acttgtagag atccaggtcg ggctcgacga
     3781 tgtagccgca ctggaagtcc atggcgacct cgaaggcccc gggccgcagc acctggccgt
     3841 aataggccgc cgattccacc acctcgtgct tgacgttgag cccgacctgc cgccactgat
     3901 cgatcatcca gatggccagc ggctcgtagg gcatcgggat cccgcggttc ttgaacgtga
     3961 aggcgaagcc gtccggcacg ccggcctcac ggaggagctt ccgggcctcg gcccgggcgg
     4021 cgttgatgtc cttgccgtac ccggccagcg aggccagctc ggcgggcggg gtggcgtacg
     4081 gcgtgcccgg cacctggacc cccgccacct ccttcacgat ggcgatctgg gagagcgcct
     4141 tggacccttc gtagcggtcc agggccaggg tgagcgcccg gcggacgcgc ctgtcgtcga
     4201 acggcttctt ctcgtggttg atggcgacca tcaggatgca gtcccaggcg ctctcctgca
     4261 ccgtgatctt gtcgccgagc gcggtcttga gggtgtcacg ctccttgggg ctgaagccgc
     4321 ggaactggat gtgggcgcgc tcgccccgga tcagggccac ctgggcggcc gagtcgcgaa
     4381 cgaacatcgc ccggaacccg tccaggtagg gcttgccctt gtcccagtag ttggggttct
     4441 tcttgcccac cacgtgcgag cccttgacgt gctcgacgaa ggtgaagggc ccggtcccca
     4501 tgatgttctt ctcgtaccag gtcatgtcct tggccaggat gtcggccttg tagatgaagt
     4561 tccacggcga ggcgatggac agcaagaacg agccctgggg gtacttgagc cggaagcgaa
     4621 cggtcgaggg atccggcgcc tcgacggcct cgacgttctg gtactggccc ttgcgactcg
     4681 agccgatccc ttgcggcggg aagatgatct tgtcgtacga ggccttgacg tccctggagg
     4741 tcatctcgct gccgtcgtga aacttcacgc cccggcggat cttgaacgtg taggtccggc
     4801 cgtcgttggt caccgtccag ctctcggcga tgctgggcac cggcttggtc ccggaggggt
     4861 cggtgggatc gacgcggagc agcgtgttgt agaacggcgc aaagggatga gtgagaccga
     4921 aggtctcctc gcggtggccg tcgtaggacg ggggctccga ggggaccggg aacaccagct
     4981 cgccgccgga gcgcggcttg tcctgggcga tcgcggggac cgccagcagg gcgatgagca
     5041 ccagggccag gaacgcgctg aggcgtcgca tcagccacct ccgtgagtag ttgtcgggtt
     5101 tcgccggaaa cattggaagt tacggcacat taaccgcggt ctgaccacgc tgtcaagcca
     5161 ggtggcaagc cgcccagtgg ccagaccgga tctcccgcag agggggtatc tgttcatggc
     5221 agcggtccac ggcgatgggg caccggggat ggaagacgca cccggacgga gggtccagcg
     5281 gactcggcac ctcgccgcgg aggaccacgc gctcacggcg ggcctccagc tccgggtccg
     5341 ggatcggcac ggcggccagc agcgccttgg tgtaaggatg cagcgggtcg tcgtagaggg
     5401 acttgcggtc ggcgatctcg gcgatcttcc ccaggtacat cacggcgacg cgatccgaga
     5461 tgtgccggac cacggcgagg tcgtgggcga tgaagaggta cgtcagaccg aactcggcct
     5521 ggaggtcctc gagcaggttg atgatttggg cctggatgga cacgtcgagg gcggagacgg
     5581 gctcatcgca gacgatcaac gccggctcca tcgcgagcgc gcgcgcgacg ccgacgcgct
     5641 ggcgctgccc gccggacatc tcgtgcgggt accgctgtgc gtgctggggc aggagtccga
     5701 cgcggcgaag cagctcaccg acgcgctggc cccgcgaccc ggcgtcgggc acgatgccgt
     5761 gcacggccag gggctcggcg atgatctggc cgacggtcat gcgagggttg agcgagctgt
     5821 aggggtcctg gaagatcacc tggaccttgc ggcgcaccgc ccgcagcgcc gcgccctcca
     5881 gggtggtgag gtcgcgcccc tcgaagatca cctgccccga cgttgcgcgc tcgagctgga
     5941 ggatgcaccg cccggtcgtc gtcttgccgc agcccgactc gcccacgagt cccagcgtct
     6001 caccgcgacg gatcgagaag ctgacgccgt ccacggcgcg cacgatgcca tgggagcggc
     6061 cgaggagccc gcccccgacc gcgaagtgct tgacgaggtc ccgaacctcc aggagcacgt
     6121 cgccgtgagg ggccagcccg gtcatcgcgc tgcgaccgcc gtgcgcgccc cgtccagccg
     6181 ctcggcttcc cagcacgcga ccacgtggcc atcgcgcggc accgcgcgca gcgccggcac
     6241 ctcggccagg cagcggtcga cgcggaaggc gcagcgtggc gcgaaggcgc agccgggggg
     6301 caggcgcgcg aggtccgggg gctggccgtc gatcggcgcc aggcgttccc ggcggggctc
     6361 gtccagcctg ggcaccgagc gcagcagtcc gagggtatag ggatgccggg gatgggcgta
     6421 aatctcgcgc gcggtgcccc gctcgatgat tttccccgca tacatgacat tcacccgatc
     6481 cgcgtaccgg gccaccaccc cgaggttgtg ggtgatgatg agcatcgcga cgcccaggcg
     6541 gcgcgacagg tccttcatga gctcgaggat ctgggcctgg atcgtcacgt cgagcgcggt
     6601 ggtgggctcg tcggcgagca ccagcgcggg ctcgcacgcg agcgccatcg cgatcatcat
     6661 gcgctgccgc atgccgccgc tgaactggtg cgggtactgg ggcaggcgcc gctgcgggtc
     6721 ggggatgccg accatcgcga gcaggtccac cgcgcggctg cgggcgtcgg tcggagacag
     6781 gccgaggtgg atctcgagcc cctcggtgag ctgacggccg atggtcaaca cggggttcag
     6841 cgaggtcatc ggctcctgga agaccatcgc gatctcgcgg ccgcgcacct tccgcatctc
     6901 ctcctcggag agcgtgagca ggtcgcgtcc cttgaagagg acctgaccgc cggcgatccg
     6961 gccggccggc ccctggatca ggcgcatgat gctcagcgcg gagacgctct tgccgcatcc
     7021 ggactcgccc acgagcgcga cggtctcccc ctcgccgacg tcccacgaga catcgtcgac
     7081 ggcgcgcacg gtgcctgctc gagtcacgaa ttgcgtgctc agcgagcgga cttcgaggag
     7141 gggcgccacg gttgcgcgag tgtaccacac gagcgcggcg gcccggcccc ggaggcgagg
     7201 ccaccgggtg cgcttctccg cgtgtgtccc tcccgccctc atgcatcggc gagcgcccga
     7261 atcactgccc ttcgagcgcc gctccgccgg cgcatcgaat cctggggggc agttaccggc
     7321 agcgggcggc ggcgaggtgc tcgccggcgg ccggcaccag accggcgtag gcgcggcaga
     7381 ccgcctcggc gacgcagggc cagcggtgct gggcggccca gcgaacgccc tctcgtccga
     7441 tgcgccagcg cagcgccgga tcggcgagca cgccgtccag ccggcgcgcc agcgccccgg
     7501 catcgccgtc ggacaccagg aacccggtca cgccgtccac caccgtcgtg gccaggccgc
     7561 ccacgcgcga ggcgatcacc gggctgccgc acgccatcgc ctcgagggcg accatcccga
     7621 acgactcgta gtacgagggc agcacggtga cgtcgctggc gacgtagtag cggcgcagcc
     7681 gctcctgcgg ctgggcgccc acgaacgtca ccacatcggc caggccgaga cgcccggcgt
     7741 ggcggcgcag ctcggcctcg tggccggcgc gcggctcgtc ggcgtcgccg ccgacgatca
     7801 gcagccgcgg cgcccgcccg tgcgcgcgca ggcgccccac ggcgtccagc agcgtgtcga
     7861 ggcccttgat gggcgcgatg cggccgacgt agagcatcag cggcccgtcg tcgaggccga
     7921 gcgctgcccg cgcctcgccg gcgtcgcccg ggcggaacag cgcggtgtcc acgccgcacg
     7981 ggatcacggt gacgcgtgcg gcctcggccc cgtgaccgat gaggtgcgca cgctcgacct
     8041 cggaggccgc gacgatcagg tcggcgtccg cgacgaggcg ggcctcttcc cgcaatctca
     8101 acaacggctc gcggtcggcg ggggagcgcg ccgccccgtt cttgagccgg ccgagggtgt
     8161 ggaacatctg cacgacgggc acggaccagc gctcgcgcag ggcgagcgcg acgacgcccg
     8221 acagccagta gtgcgcgtgc acgagatcgt actcgacgcc acgggcgatc cgccaggcct
     8281 ccacaccgtc gaggaactcg tcgaggtgct cgtgcacgcg ctcgcggagc agcggcgcct
     8341 cgggcccggc cggcaaatgc acgacgcgca cggtctcggc catctcgacg acgcgcggga
     8401 tcgcctcgtt ctgggagcgc gtgaagatgt cgagggcggc gcccatgcgc ccgagctcgc
     8461 gggacagctc gcgcacgtac acgttcatcc cgccggtctc cttgccgccg agcgcggcga
     8521 gcgggcaggt gtgcacgctg agcatcgcga cgcggatcat ggtagcgccg gcgagatcgt
     8581 cacccggtac acgtcttccg ggacgggccc gaagtcgtac ttgtagcgct cctcgccgcg
     8641 caggaaatcg aagcggcgcc ggccgcgggc gatggcgtcc tggacgaggt agccgagcag
     8701 gacgacgccc ggcgagagcg ccgcgtgatc ggggtgaaac ccggagttgt agagcccgac
     8761 gacgccgtcc cactcgagcg tgatgaaagc cgcgagcggg cccccgcgcg tgtcgaggag
     8821 ccagagccgc gccatgccga cctcggcgag ggcggtcacc gcgcggcgga agaaggcctc
     8881 catgcgctgg tccatgaagc gcgccttgcc ggcgcgcgag cgccggtgca gcccgaggaa
     8941 gtcatccagc cggatctcga cgtccgcccg cgtcgccgcg cacgtggccc ccgcttcggg
     9001 cacctcgcgc gcgagacggc gcatcttccg ggtcagctcg tgacgctgct tgccggagag
     9061 ggtggccagg tagtcgtccc acgaggcggg cagctcgagg accgggcaac gctcctccac
     9121 cgcctccgtc acggcgagcc cgaaggcggc cgcgagcccc ggcactgcgg tcacggtcgc
     9181 cgacgccgcg ggcaccgcgt gcaggtccca cgcggcgggc gtcgtggcgc gcgcggcgag
     9241 gagggcggac caggcctcct cctcgcgccc cgactcggcg acgaggtcga ggtagtccga
     9301 gacgtcggct ccgcctagca gctccatcgc cgccgggccg gtctcgtaga gcggcaacag
     9361 cgccacggtc tcgccgtgat cctcgacgcg ccacacctcg agccgccgtc cgtgcgcgaa
     9421 ggtgcgtgcc cactcgctct gccacgtcca cgtgagaaag ggcgagcgga ggcgggtgcg
     9481 gcgatgcagg gcgtcccacg caggggggcc gaccgcctcg aggcggtcgt ggagggcgat
     9541 cttcacaact cggtgtcggc tgtgccggag cccgacgtgg cctccgcgtc gggcgcggcg
     9601 acgtcgtcct cggaccccgc catcgcctcg tcgacggctt cgtcgaggtc gtcgcccagg
     9661 tcgtcgccca tctcgcggcc catcttcttc atgaaccgcg cgacgctggc ggggtcgttc
     9721 tcgtcgatgt ctccgaagct cgcgtcgccc gcgagcgcgt ccagacgcgc ctcctccgac
     9781 ttgacggtcg cgaatcgcga catgaggcgg gtgagcgcgg cgccgccgca ccggggacat
     9841 cggggagcct cggcggccgc cggacggagc acgaggaggc tgaccacgcg gcggcagtcg
     9901 cggcactcgt actcgtagat cggcatcccc ggcaattcta gcaccggctt ccaggggtgt
     9961 ctcgatggcc gacatcgatg actcgcgcgt gtcagcaagg cgcacacgcg cgcgtggcac
    10021 agccgccgaa accccgcgtg gatcaaccga aaccccgcgc cgatcaacgc ctgggcggcg
    10081 ggcactcttc ttgctcggcc agtccgtgtc agcgtggacc tggcccggag gtcgaactgg
    10141 cgatgaccga accgacggac ctgaccgagc tacggggcga tagcgagcgc ctgttgatcg
    10201 aggccgtggt cgagctggcg cgcacgctgc gcgacgagcg ggaccgcctg gccgggcgcc
    10261 tccgccaggt ggagagcgag ctcaccacgc tgacggagcg cctggaagcc tcgcggaccg
    10321 ccccccccgg cgcctgacgc cgtcggtcag cgccgtccgt ccgcgcccac gaacatcgcc
    10381 tccatccggt ccaccgcccg attccagtcg aatgccgtcg tgacgagcag ctgccccgcc
    10441 accgcgatct tccgccggag cgtggcgtcc gccgcgaggc gctcgagctt cgtggcgaga
    10501 tcggcgatgt cgcgacggcg cgcgacgagc gcggtttcgc cgtcgcgcgc gtagtcgcgg
    10561 cagcctccgt tgtcgtaggt gacgagcgcc gcgccgcagg ccatggcctc catcggcggc
    10621 atccccagcc cttcgtccca cgacggacac aggtagatct cgcagttcga gtagacctcg
    10681 cgcagcgtct cctggggcgg atcggcgtgg aactcgtcgt acctcatggc acggcggggc
    10741 ggcttgacgc ccaggccgac caggaacagc gaaggcagcg agcgccgcgc gaccgcgacg
    10801 gcctccaggc cgtccgcgac acccttccag gcgtagtcgt gatggagcat gagcacgcgc
    10861 gggcgcggtg tcgcgaccgt cgccggcacg gggtggaaga gcgcggtgtc gacgggggtc
    10921 acgagcacct cgggctcgct cgcgaatttc tcgcgcatca cgtcacgcag ccaggtcgag
    10981 acgacgatct tcgcgagcgg caggcggtag gtggcgtcca cccggtcggg cgcgccgtgg
    11041 tagaggctct cgtagtgctg gatcaggtag aacttcgcgc cgcagcgggc gggcgcctcg
    11101 gccaccgccg ccgccgtctg ccacgcggtc gcgacgacca cgtctcccgc gggcacgctc
    11161 tccggggaga aatccggcat ccactgcacc ctcggcctga accccggcac ccagtccggt
    11221 ccgcggccgt ggacgttgcg ccaccacgcc tgccacggcc ggcgcacggg gaccagcacg
    11281 gtcacgtcgt gcccccgcgc ggcgaggcgg tccgcgtacg tgaggaccgc gcgcacgccg
    11341 cccgcgatcc cgaggtgcgg gcagaagaac gtgatgcgca cgcgctcaga tcgccatcga
    11401 cacctgcccc gtgcgccggt aggccgcggg gtcgagcggc acgttgatgc accagacctc
    11461 gtcggagccg agcgctcgct cgagcgccgg ccggatggcg gcggggtcgg tgaccagcac
    11521 gccgcggccg cccagggcct cgaccatgag gtcgtagcgg gtcgtcggca gcgacgtcgc
    11581 gaccgcgcgc tcctccccga agaacgagag ctgcgggttg cggatctggc cccagccgcc
    11641 gtcgttgccg atgatgca
//
LOCUS       DATIAZ010000017         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_661301, whole genome shotgun sequence.
ACCESSION   DATIAZ010000017 DATIAZ010000000
VERSION     DATIAZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661301"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..723)
                     /gene="lepB"
                     /locus_tag="VLF19_00600"
     CDS             complement(4..723)
                     /gene="lepB"
                     /locus_tag="VLF19_00600"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSE91774.1"
                     /translation="MDDRKLEAEVERTRIGVSRSGGSTAEPAEAPVTLGVRRKSLVRE
                     YGEAIVIAILLALVIRTLVVQAFTIPSGSMMDTLLVGDYILVNKFLYGPEIPFTEWRT
                     PGLRQPRRGDIIVFKYPQDEKRDFIKRIVGTPGDEVQIRGHDVYVNGGKLAEPYVRQG
                     GPLVHAGGGYCGYAYGCEPTRVPSDSYFVMGDNRDNSQDSRYWGFVKRDKIKGKAFLI
                     YWSWDSDRHWLRWWRLGNYIP"
     gene            complement(725..2515)
                     /gene="lepA"
                     /locus_tag="VLF19_00605"
     CDS             complement(725..2515)
                     /gene="lepA"
                     /locus_tag="VLF19_00605"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSE91775.1"
                     /translation="MTLERIRNFSIVAHIDHGKSTLADRLLALTGTMDAKKAVDQVLD
                     SMDLERERGITIKAHTVRLLYRARDGQEYTLNLIDTPGHVDFSYEVSRALAACEGAIL
                     IIDAAQGIEAQTLANYYLAFEAGLTIVPVINKIDLPAADVEGTRAQITEVLDLDGDEA
                     LAISAKHGTGVPEVLEAIVHRIPPPVGDAEAPLKALVFDSFYDSYQGVVVYVRLTDGR
                     VRAGTKILLMSNGRSCDVQQVGVFTPEMRPVEELSAGQVGFLTASIKRVADAKVGETI
                     TAAARPTEEAFPGYRDAKPMVFAGLYPIEDTDYEALRDALEKLRLNDPAFTFEPETSL
                     ALGYGFRCGFLGMLHMEIVQERLEREFNLSLIVTAPSVRYRVLTTAGDVMEIDNPAKL
                     PTPDRIGQMEEPYVTSSIFAPTEFMTAIYKLAQDKRAEHRSLEYLGKRVHIRFDFPLA
                     EIVADFYDKLKSISKGYASFDYEFADFRPSDLVKLDILLNGDPVDALSIIVHRDKAYE
                     KGKALVEKLRTVIPRQLFEVAIQAAIGSRVIARETVKAMGKNVTAKCYGGDITRKRKL
                     LERQKEGKKRMKQVGRVEVPQEAFLTVLKS"
     gene            complement(2568..>2787)
                     /locus_tag="VLF19_00610"
     CDS             complement(2568..>2787)
                     /locus_tag="VLF19_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91776.1"
                     /translation="GPRAVLGAGCVVAAGATVEEAVLWERVEVGPGAVLRGCVVVSDA
                     RIGAGAEVGAGVVVESGAVVADHARPTV"
BASE COUNT          434 a          950 c          933 g          470 t
ORIGIN      
        1 acctcacggg atatagtttc ccagccgcca ccagcgaagc cagtgacggt cgctgtccca
       61 cgaccagtag atcaggaagg ccttgccctt gatcttgtcc cgcttcacga acccccagta
      121 ccggctgtcc tgggagttgt cgcggttgtc gcccatcacg aagtacgagt cgctcggcac
      181 ccgcgtcggc tcgcagccgt acgcatagcc acagtagccg ccgcccgcgt ggacgagtgg
      241 tccgccctga cgcacgtagg gctccgcgag cttgccgccg ttgacgtaga cgtcgtggcc
      301 gcggatctgt acctcgtcgc ccggcgtccc cacgatccgc ttgatgaagt cgcgcttctc
      361 gtcctgagga tacttgaaga cgatgatgtc gccgcgccgg ggctggcgga ggcccggggt
      421 gcgccactcc gtgaagggga tctcggggcc gtagaggaac ttgttcacga ggatgtagtc
      481 accgaccagc agcgtgtcca tcatcgagcc cgacggaatc gtgaacgcct gcacgacgag
      541 ggtacggatg accagtgcca ggagaatggc gatgacgatc gcctcgccgt actcccggac
      601 cagcgacttg cggcgcacgc cgagggtcac cggagcctcg gcgggctcgg cggtcgaccc
      661 ccccgaccgg gagaccccga tccgcgtccg ctcgacctcg gcctcgagtt tgcggtcgtc
      721 catctcagct cttcagcacc gtcaggaacg cctcctgggg gacttcgacc cggcccacct
      781 gcttcatgcg cttcttccct tccttctgcc gctccagcag cttgcgcttg cgcgtgatgt
      841 cgcccccgta gcacttggcg gtcacgttct tgcccatcgc cttcacggtc tcccgggcga
      901 tcacgcggct gccgatggcc gcctggatgg ccacctcgaa cagctggcgc gggatcaccg
      961 tccggagctt ctcgacgagc gccttgccct tctcgtacgc cttgtcgcgg tggacgatga
     1021 tggacagcgc gtcgacaggg tcgccgttga ggaggatgtc gagcttgacg agatccgaag
     1081 ggcgaaagtc tgcaaactcg tagtcgaacg acgcgtaccc cttggagatc gacttgagct
     1141 tgtcgtagaa gtccgcgacg atctcggcga gcgggaagtc gaagcggatg tggacccgct
     1201 tgccgagata ctcgagggac cggtgctcgg cgcgcttgtc ctgggcgagc ttgtagatcg
     1261 ccgtcatgaa ctccgtcggc gcgaagatcg acgaggtgac gtacggctcc tccatctgcc
     1321 cgatccggtc cggagtcggc agcttggcgg gattgtcgat ctccatgacg tcgcccgcgg
     1381 tggtgagcac gcggtagcgc accgacggcg cggtgacgat cagcgagagg ttgaactcgc
     1441 gctccagccg ctcctgcacg atctccatgt gcagcatgcc gaggaacccg cagcggaagc
     1501 cgtagccgag ggccagcgag gtctcgggct cgaaggtgaa ggccggatcg ttcagccgga
     1561 gcttctccag cgcgtcgcgc agcgcctcgt agtccgtgtc ctcgatggga tagagcccgg
     1621 cgaacaccat gggcttggcg tcccggtagc cgggaaacgc ctcttcggtg ggccgggcgg
     1681 ccgccgtgat cgtctcgccc acctttgcgt cggccacgcg cttgatcgag gcggtgagaa
     1741 agcccacctg accggccgac aactcctcca cgggccgcat ctcgggcgtg aagacgccga
     1801 cctgctggac gtcgcaggag cggccgttgg acatgaggag gatcttcgtg ccggcccgca
     1861 cgcgtccgtc cgtgagccgt acgtacacca cgacgccctg gtaggagtcg tagaaggagt
     1921 cgaagaccag tgccttcagc ggtgcctcgg cgtcgccgac gggcggcggg atgcggtgga
     1981 cgatcgcctc cagcacctcg ggtacgccgg tgccgtgctt ggcggagatc gccagcgcct
     2041 cgtcgccgtc gaggtcgagc acctcggtga tctgcgcgcg cgtaccctcc acgtcggccg
     2101 cgggcaggtc gatcttgttg atgacgggga cgatggtgag gcccgcctcg aacgccaggt
     2161 agtagttcgc gagcgtctgg gcctcgatgc cctgcgcggc gtcgatgatc aggatagcgc
     2221 cctcgcaggc cgcgagcgcc cgcgacacct cgtacgagaa gtcgacgtga cccggcgtgt
     2281 cgatgaggtt gagcgtgtac tcctggccgt cgcgcgcccg atagagcagg cgcaccgtgt
     2341 gggccttgat cgtgatgccc cgctcgcgct cgaggtccat cgagtccagc acctgatcga
     2401 cggccttctt ggcgtccatg gtgcctgtga gagcgagcag ccggtcggcg agcgtggact
     2461 tgccgtggtc gatgtgggcg acgatcgaga aattccggat ccgctcgagt gtcacaacgt
     2521 ctcagtctaa cacgcgggct ctcgcgccgg gccgacggtg cctcccgtca gacggtcggt
     2581 cgcgcgtggt cggcgacgac ggcgcccgac tccaccacca caccggcgcc gacctccgcg
     2641 ccggctccga tccgggcatc ggacaccacg acgcatccgc gcaggaccgc gcctggcccc
     2701 acctcgacgc gctcccacag cacggcctcc tcgaccgtgg cgccggcggc gacgacgcag
     2761 ccggcgccga ggaccgcccg cggcccg
//
LOCUS       DATIAZ010000018         4478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_398201, whole genome shotgun sequence.
ACCESSION   DATIAZ010000018 DATIAZ010000000
VERSION     DATIAZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398201"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /gene="mraY"
                     /locus_tag="VLF19_00615"
     CDS             complement(<1..1071)
                     /gene="mraY"
                     /locus_tag="VLF19_00615"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSE91777.1"
                     /translation="MLYHLLVPLARDHIVFNVFRYLTFRSVMALITALVISLLIAPWI
                     IHRLHAWQQGGDTVREDTPERHQQKKGTPTMGGLVILLALLLSTLLWANLTNRYVWVV
                     VLATAGFGLIGLWDDVRKLRRRKGLTGRTKLGLQFALALALVQIVFWQAPASGWGPTL
                     AIPFLKGWLLDLGWWWIPFAMLVIVGASNAVNLTDGLDGLAIGPIIMAGGAFGVIAYL
                     TGNFRAADYLKIVNVRGAGELTVFCGALIGAAIGFLWFNCHPAQVFMGDVGSLALGGA
                     IGTLAVLTKAELLLPLIGGLYVVEAASVIIQVVSFRLTGRRVFRMAPLHHHYELTGWA
                     EPKIVVRFWIVSFALALLALTTL"
     gene            complement(1081..2487)
                     /gene="murF"
                     /locus_tag="VLF19_00620"
     CDS             complement(1081..2487)
                     /gene="murF"
                     /locus_tag="VLF19_00620"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSE91778.1"
                     /translation="MRAALAAFTVQEIVRATQGAMVVGDLAIPVTGVGIDSRSLGVGE
                     AFFAIRGHRLDGHQFVADAASRGAACLVVHTVPDPIPAGVPLVLVEDTTKALGRLAAA
                     HRRKFDIPVVAVTGSNGKTTTKEMTAVVLATRWRVLKPEGSFNNQWGLPLTLLRLSPD
                     HEALVVELGTNQPGEIAALSEIAAPTVGIVTTVAAVHTEFLGSLDGVRDEKAALVRAL
                     PRDGVAVLNADDPRVSGMARDTVARVVTYGGAEGAAVRVAGDVVEEAAGLAFVVQAGD
                     ARERVRLAFAGRHNVGNALAAAAAGLALGLPLSDVVRGLEAARPAKGRCVWRRAGDIL
                     VLDDTYNANPASVAAALDTASASRGGRRLVVVLGDMLELGPTADEAHREVGRRVAESG
                     AAELVGLGPRMRLAVEGARAAGLAEAHHLATFEDAVAHLLKRVAPGDLVLVKGSRGMR
                     MERVADALTARLARPGEA"
     gene            complement(2484..3965)
                     /locus_tag="VLF19_00625"
     CDS             complement(2484..3965)
                     /locus_tag="VLF19_00625"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSE91779.1"
                     /translation="MPVTDLLAALPDSTVLGTPPSTVTGIADDSRKVAPGDCFVAVPG
                     LKHDARRFVPEAVARGAALVVTEGDALPGVPVAQVLVGSAREALGRLAGAYHGHPSRR
                     LTVVGITGTNGKTTTSYLVEALLHARGLKTGVIGTIQYRLGERTLPAGQTTPDALALQ
                     AMLAAMSEDGVGGVAMEVSSHALAQGRIEGLAIDVAVFTNLTQDHLDFHGTLEAYRRA
                     KRRLFELLERSPKPGRTAVVNADDPSGREMTGGLSVPVLTFGLRPGASIRAREWSSTL
                     EGVRLRADTPHGEIALASPLIGEHNVMNLLGAIGVGLALGLAPDAAARALAAVGTVPG
                     RFEQVREGQSFLVVVDYAHTPDALDRVLATARKLTAGRLAVVFGCGGDRDRGKRPIMG
                     EIAARVADRVWVTSDNPRSERPEAIVEEIAAGARRAGGGPGKVVTWTDRRGAIEDALG
                     WARAGDTVVIAGKGHETYQIVGGETFPFDDRAVARQALRARRG"
     gene            complement(3956..>4478)
                     /locus_tag="VLF19_00630"
     CDS             complement(3956..>4478)
                     /locus_tag="VLF19_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HSE91780.1"
                     /translation="RRYSRAPGVLSFVGFAPADEPRFVMLVLLDEPKTERWGSEAAAP
                     IFASVGREILRYLEVPPRDAQPVQIVRGSGAEVPAATRVRLVSMAPDVHEPGVVPDLR
                     GRPLRRALDTLAALGLGVAVTGRGVVVQQSPAPGGAIEPGGRVQLTLAAPPHGTRATA
                     VSGRGEPCESPCR"
BASE COUNT          637 a         1683 c         1607 g          551 t
ORIGIN      
        1 cagggtcgtc aacgccagca gcgccagcgc gaacgagacg atccagaagc gcacgacgat
       61 cttgggctcc gcccagccgg tcagctcgta gtggtggtgg agcggagcca tgcggaagac
      121 gcggcgtccg gtgagcctga agctgacgac ctggatgatg acggacgccg cctcgacgac
      181 gtagaggccg ccgatcagcg gcaacagcag ctcggccttg gtcagcacgg cgagcgtgcc
      241 gatcgcgccc ccgagggcca gcgagcccac gtcgcccatg aatacctggg ccgggtgaca
      301 gttgaaccag aggaagccga tggcggcgcc gatcagcgcc ccgcagaaca cggtcagctc
      361 gccggcgccg cggacgttca cgatcttgag gtagtccgcc gcccggaagt tgcccgtgag
      421 gtatgcgatc acgccgaagg cgccgccggc catgatgatc ggcccgatcg ccagcccgtc
      481 gaggccgtcg gtcaggttga ccgcgttcga ggcgcccacg atcaccagca tcgcgaacgg
      541 gatccaccac cagccgaggt cgagcagcca gcccttgagg aagggaatgg ccagggtcgg
      601 gccccagccg ctcgccggcg cctgccagaa gacgatctgc acgagggcca gcgccagcgc
      661 gaactgcagg ccgagcttcg tgcgcccggt gaggcccttg cggcgccgga gcttcctcac
      721 gtcgtcccag aggccgatga gaccgaagcc cgccgtggcg agcaccacca cccagacgta
      781 gcggttcgtg agattcgccc agagcagcgt cgagagcagg agcgccagga ggatcaccag
      841 gccgcccatg gtcggcgtgc ccttcttctg ctggtgacgc tccggcgtgt cctcccgcac
      901 cgtgtcgccg ccctgctgcc aggcgtggag ccggtggatg atccacggcg cgatcaagag
      961 cgagatcacc agcgccgtga tgagggccat taccgaccgg aacgtcaggt acctgaagac
     1021 gttgaagacg atgtggtccc tggcgagcgg gacgagcagg tggtacagca tccgacgtcc
     1081 tcatgcctct ccgggccggg ccagccgcgc ggtgagggcg tccgcgacgc gctccatgcg
     1141 catgcctcgc gagcctttga ccaggaccag gtcccccggc gccacccgct tgagcaggtg
     1201 cgcgaccgcg tcctcgaacg tcgccaggtg gtgggcctcc gcgagaccgg ccgcccgggc
     1261 gccctcgacg gccaggcgca tgcgcgggcc caggcccacc agctccgcgg cgccgctctc
     1321 ggccacgcgc cgtcccactt cgcgatgcgc ctcgtcggcg gtggggccga gctcgagcat
     1381 gtcgccgagc acgacgacga gccggcggcc gccgcgggac gccgacgccg tgtcgagggc
     1441 cgcggccacc gaggccgggt tcgcgttgta ggtgtcgtcc agcacgagga tgtcgccggc
     1501 gcggcgccac acgcagcgcc ccttcgccgg tcgcgcggcc tcgagcccgc gcaccacgtc
     1561 ggagagcggc agccccagcg cgaggccggc ggcagccgcg gccagggcgt tgccgacgtt
     1621 gtggcgcccc gcgaaggcca ggcgcacgcg ctcgcgtgcg tccccggcct ggacgacgaa
     1681 cgccagcccc gccgcctcct cgacgacgtc cccggccacc cggaccgcgg ctccctcggc
     1741 cccgccgtac gtcacgaccc gcgccacggt gtcgcgcgcc atgccgctga cccgcgggtc
     1801 gtcggcattg agcacggcca cgccgtcgcg aggcagcgcg cgcacgagcg ccgccttctc
     1861 gtctcgcacg ccgtcgagcg atccgaggaa ctcggtgtgc acggcggcga cggtggtgac
     1921 gatgcccacc gtgggcgcgg cgatctcgga cagggcggcg atctcgccgg gctggttcgt
     1981 gccgagctcc acgaccagcg cctcgtggtc gggcgagagg cgcagcaggg tcaggggcag
     2041 cccccactgg ttgttgaagc ttccctcggg cttgagcacg cgccagcgcg tcgccagcac
     2101 gacggcggtc atctccttgg tcgtggtctt gccgttcgag ccggtcacgg cgacgacggg
     2161 gatgtcgaac ttccgccggt gcgcggcggc cagccgcccg agcgccttcg tggtgtcctc
     2221 gacgaggacg agcggcactc ccgccggaat cgggtccggg acggtgtgga ccaccaggca
     2281 ggcggcgccg cggctcgcgg cgtcggcgac gaactgatgg ccgtcgagcc ggtggccacg
     2341 gatcgcgaag aaggcctcgc cgacgccgag gctgcgcgag tcgatgccga cgccggtgac
     2401 cggaatggcg agatcgccca ccaccatcgc accctgggtc gcccggacga tctcctggac
     2461 ggtgaacgcc gccaaggccg ccctcacccg cgccgcgcgc gcagcgcctg ccgcgccacg
     2521 gcgcgatcgt cgaaggggaa tgtctcgccg ccgacgatct ggtacgtttc gtgtcccttg
     2581 ccggcgatga cgacggtgtc gccggcccgt gcccagccca gcgcgtcctc gatggcgccc
     2641 cggcggtcgg tccaggtgac gaccttgccc ggccccccgc ccgctcgccg cgcgcccgcg
     2701 gcgatctcct ccacgatcgc ctcggggcgc tccgatcggg gattgtcgga cgtgacccac
     2761 acgcggtcgg cgacgcgcgc ggcgatctct cccatgatcg ggcgcttgcc gcggtcgcgg
     2821 tcgccgccgc agccgaagac gacggcgaga cgccccgccg tcagcttgcg ggccgtcgcg
     2881 agcacgcggt cgagcgcgtc gggcgtgtgc gcgtagtcga cgacgacgag gaacgactgc
     2941 ccttcacgga cctgctcgaa ccgcccgggc acggtgccga ccgcggcgag cgcccgcgcg
     3001 gcggcgtcgg gagccaggcc gagcgccagg ccgacgccga tggcgccgag caggttcatc
     3061 acgttgtgct ccccgatgag cggcgaggcg agcgcgatct cgccgtgcgg cgtgtcggcg
     3121 cggagccgca cgccctccag cgtcgaggac cattcgcgag cgcggatcga cgcgcccgga
     3181 cgcaggccga acgtcagcac gggcacggac agccctcccg tcatctcgcg tccggacgga
     3241 tcgtcggcgt tcaccaccgc ggtccgtcca ggcttgggcg accgctccag cagctcgaag
     3301 agacgccgct tggcccgccg gtacgcctcc agcgtgccgt ggaagtcgag atggtcctgc
     3361 gtcagattcg tgaacacggc gacgtcgatc gcgagcccct cgatgcgccc ctgcgccagc
     3421 gcgtgggaag agacctccat cgcgaccccg cccacgccgt cctcggacat ggcggccagc
     3481 atggcctgga gcgccagcgc gtcgggggtc gtctgcccgg cgggcagcgt ccgctcgccg
     3541 agccgatact ggatggtccc gatcacaccc gtcttgagcc cgcgggcgtg caagagggct
     3601 tccacgagat aagacgtggt cgtcttgccg tttgttcccg tgatgcccac gacggtgagc
     3661 cgacgcgacg gatgtccgtg gtacgcgccg gcgaggcgcc cgagtgcctc gcgcgcggac
     3721 ccgacgagca cttgcgccac cggcacgccc ggcaaggcgt cgccctcggt cacgacgagg
     3781 gcggcccccc gcgcgacggc ctcgggcacg aagcgccgcg cgtcgtgctt gagccccggc
     3841 acggccacga agcagtcgcc gggcgcgacc ttgcgcgagt cgtcggcgat gcccgtcacg
     3901 gtcgacggcg gtgtgccgag caccgtggag tccggcaacg ccgcgagcag gtcggtcacc
     3961 ggcatgggct ctcgcacggc tctccccgcc ccgataccgc ggtcgcccgc gtgccgtgcg
     4021 gcggcgccgc gagcgtgagc tgcacgcgcc cccccggctc gatcgcgccg ccgggcgccg
     4081 gcgactgctg caccaccacc ccgcgccccg tgacggcgac gccgagtccg agcgcggcca
     4141 gcgtgtcaag cgcccggcgc agcgggcggc cgcgcaggtc cggcacgacg ccgggctcgt
     4201 ggacgtccgg cgccatgctc acgagccgca cgcgcgtcgc cgccgggacc tcggcaccgg
     4261 acccgcggac gatctgcacc ggctgcgcgt cgcgcggcgg cacctcgagg tacctcaata
     4321 tttctcggcc gacggacgcg aagatcggcg ccgccgcctc gctgccccag cgctcggtct
     4381 tcggctcgtc gagcaggacg agcatcacga agcgcggctc gtcggccggc gcgaagccca
     4441 cgaaggacag caccccgggc gcgcgcgagt agcgccgc
//
LOCUS       DATIAZ010000019         4404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1187911, whole genome shotgun sequence.
ACCESSION   DATIAZ010000019 DATIAZ010000000
VERSION     DATIAZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187911"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1071)
                     /gene="trpS"
                     /locus_tag="VLF19_00635"
     CDS             complement(79..1071)
                     /gene="trpS"
                     /locus_tag="VLF19_00635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSE91781.1"
                     /translation="MTATRERVLSGMRPTGKVHLGNHLGALDNWVRLQEAYDCFFFVA
                     NWHALTTDVENVREVPAATIEMLADWLGAGLDPQRATLFVQSWVPEHAELHLLFSMIT
                     PVGWLERVPTYKEMIEQLGLESPSYGFLGYPLLQAADILMYKAKWVPVGIDQVPHVEL
                     TREVARRFNHSFAAVFPEPQAKLTEIPKVPGTDGRKMSKSYGNAIYLSDAEATIREKV
                     LPMITDPARRRRSDPGNPDVCPVFDLHRIFTPSADREWAATGCRTAGIGCLDCKSKLL
                     EHMLPALAPIRERRQQYAEKPHVIVDILREGSARARAVARQTMEDVRRVVTLTP"
     gene            complement(1068..1823)
                     /locus_tag="VLF19_00640"
     CDS             complement(1068..1823)
                     /locus_tag="VLF19_00640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSE91782.1"
                     /translation="MTAARLSAVVVTLNEEERLRACLESVAWADEIVVVDAESHDKTV
                     QIAREFTDRVLVRPWPGFAAQKNFGLEQATGDWLLSLDADEEVSPALREELGALLRAP
                     ATVDGYAVPRRNIFLGQWIRHGGLYPDWQVRLFRAGRGRFVARDVHESVEVEGAVGRL
                     AGHLVHRSYRDLSDFFERAHRYSTLAARDLVRRGRTARARELLVRPVGRFLGMYLVQR
                     GFLDGRKGLLLAALYAYYVFMRTAKVWEVTRRR"
     gene            complement(1820..2821)
                     /gene="waaC"
                     /locus_tag="VLF19_00645"
     CDS             complement(1820..2821)
                     /gene="waaC"
                     /locus_tag="VLF19_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HSE91783.1"
                     /translation="MNVVIVKLSSIGDVVHALPVAATLRARLPGARITWLCERREAVV
                     LVGHPAIDEIVVADTRGWRRARGLDAMRAVASGFVALARQLRSGRFDVALDLQGLLKS
                     GLLTACTRAPLRIGFESSRCRERVNALFTNRRVLPPESARHVADQYLALLEPLGVAGP
                     VREFRLPCDPAAEAAIDDVFSAAGLKPRNRVVVLNPGAARADKRWPVERFRSLGARLA
                     TEAGAQVIVVWGPGEASAARAIAGPAGGRVLLAPPTDIHQLIAVLRRASLLVAADTGP
                     LHLAAAIGTPCVGLYGPTRAERNGPYGSGHRALEGREGRVGAIGVEDVLRAAVELLG"
     gene            complement(2818..3315)
                     /locus_tag="VLF19_00650"
     CDS             complement(2818..3315)
                     /locus_tag="VLF19_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HSE91784.1"
                     /translation="MSGAAPLSVDEAAALAGRLRAAGRRIILANGCFDLLHVGHVRYL
                     DGARALGDVLFVGINSDAAVARLKGAGRPLMPAGERAELLAALRAVDHVVVFDEDTAD
                     GLIARLRPHVHAKGTDYTAQSVPERDTARAVGADVAIAGDPKGHSTRDLIARIRARFP
                     GGSRA"
     gene            complement(3312..4313)
                     /locus_tag="VLF19_00655"
     CDS             complement(3312..4313)
                     /locus_tag="VLF19_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSE91785.1"
                     /translation="MTPSERLRAAIDLFAGRVVAVVGDLITDEYLIGKPGRISREAPV
                     IILRFTDRDVRLGGAANAAHNVHALGARVVPIGVVGRDVAGEELMALFRGVGIPTDGL
                     VVEAGRTTPVKTRIMAGGYQATRQQVVRMDREPTGELAPTTEDALLARVTALAERADA
                     IVVSDYGYGTVTPRVFERVRAIARKRGNVVTVDSRYELPRFIGVTAATPNEAELGELT
                     GAPVDDERAVEKAGRQLLERLDAGMLLVTRGSQGLALLEREGAATFIPIFGTDEIADV
                     TGAGDTVISAFTVGLASGASPVEAATLANMAGGLVVMKRGTATVSRVELAQALGAAS"
BASE COUNT          620 a         1623 c         1578 g          583 t
ORIGIN      
        1 ggagcaggtc gaaggggcct tcgaacggcc cgacgcgcag cgtcagcccg gacgtggcgc
       61 ctgccgtggc cgtctcggtc atggcgtcaa cgtgacgacc cggcgcacgt cctccatcgt
      121 ctgccgagcg acggcgcggg cgcgcgcaga gccttcgcgc aggatgtcga cgatcacgtg
      181 gggcttctcc gcgtactgct ggcggcgctc gcggatcggc gcgagcgccg ggagcatatg
      241 ctcgagcagc ttgctcttgc agtcgaggca tccgatgccg gcggtgcggc agcccgtggc
      301 cgcccactcg cggtcggccg acggcgtgaa gatcctgtgc aggtcgaaca ccggacagac
      361 gtcggggttg ccgggatccg accgccgccg tcgcgccggg tcggtgatca tcgggagcac
      421 cttctcgcgg atcgtcgctt cggcgtccga caggtagatc gcgttgccgt acgatttcga
      481 catcttgcgc ccgtcggtcc ccgggacctt ggggatctcc gtgagcttgg cctggggctc
      541 cgggaagacc gcggcgaagc tgtggttgaa gcgccgcgcc acctcgcgcg tcagctccac
      601 gtgcggcacc tggtcgatgc ccaccggcac ccacttcgcc ttgtacatga ggatgtcggc
      661 ggcctgcagc agcggatagc cgaggaaccc gtaggacggc gactcgagcc cgagctgctc
      721 gatcatctcc ttgtacgtgg gcacccgctc gagccatccc accggggtga tcatcgagaa
      781 gagcaggtgc agctcggcgt gctccggcac ccaggactgt acgaacagcg tcgcgcgctg
      841 cggatcgagg cccgccccga gccagtcggc gagcatctcg atggtcgccg ccggcacctc
      901 gcggacgttc tccacgtcgg tggtgagcgc gtgccagttg gcgacgaaga agaagcagtc
      961 gtaggcttcc tgcaacctca cccagttgtc gagcgcaccg aggtggttgc cgaggtgcac
     1021 cttgccggtc ggccgcatgc ccgacagcac gcgctcccgc gtcgccgtca tcgccgtctc
     1081 gtgacctccc acaccttggc cgttcgcatg aagacatagt acgcgtagag cgcggcgagc
     1141 agaaggccct tgcgcccgtc gaggaagccc cgctgcacga ggtacatccc caggaaccgc
     1201 ccgaccggcc ggacgagcag ctcgcgcgct cgcgccgtcc ggccccgacg caccagatcg
     1261 cgcgcggcca gcgtcgagta gcggtgggca cgctcgaaga agtcactcag gtcccgatag
     1321 gaacggtgca cgaggtggcc ggcgagacgg ccgacggcgc cctcgacctc gacggactcg
     1381 tgcacgtcgc gcgcgacgaa gcgcccccgc ccggcgcgga acagtcgcac ctgccagtcc
     1441 ggatagagcc cgccgtggcg gatccactgg ccgaggaaga tgttgcggcg gggcacggcg
     1501 tagccgtcca cggtcgccgg cgcgcggagc aaggcgccca gctcctcgcg cagcgccggc
     1561 gacacctcct cgtcggcgtc gagcgacagg agccagtcgc cggtcgcctg ctccaggccg
     1621 aagttcttct gcgcggcgaa gcccggccag gggcgcacca gcacccggtc ggtgaactcg
     1681 cgcgcgatct ggaccgtctt gtcgtgggat tcggcgtcca cgacgacgat ctcgtcggcc
     1741 caggccacgc tctcgagaca ggcccgcagg cgttcctcct cgttcagggt cacgaccacg
     1801 gcgctcaggc gcgcggccgt catccgagca gctccacggc ggcgcgcagg acgtcctcga
     1861 ccccgatggc gccgacgcgg ccctcgcgtc cttccagcgc ccggtggccg cttccgtagg
     1921 gaccgttgcg ctcggcgcgg gtggggccgt agaggccgac gcacggcgtg ccgatagccg
     1981 cggccagatg gagcggcccc gtgtcggcag ccacgagcag gctcgcccgg cgcaggacgg
     2041 cgatgagctg gtggatgtcc gtgggcggcg ccagcagcac gcgtccgccg gcgggaccgg
     2101 cgatcgcgcg cgcggcgctc gcctcacccg ggccccacac cacgatcacc tgggcgcccg
     2161 cctccgtcgc caggcgcgcg cccagggacc ggaagcgctc caccggccag cgcttgtcgg
     2221 cccgtgctgc gccggggttg agcacgacga cgcggttgcg cggcttgagc cccgccgccg
     2281 agaacacatc gtcgatcgcc gcctcggcgg ccggatcgca cgggagccgg aactcgcgca
     2341 ccggcccggc cacgccgagc ggctcgagca gcgcgaggta ctggtccgcg acgtgtctcg
     2401 ccgactccgg cgggagcacg cgccgattcg tgaagagcgc gttgacccgc tcgcggcagc
     2461 gggacgactc gaacccgatg cggagcggcg ctctcgtgca cgccgtgagc aggccgctct
     2521 tcagcaaccc ctgcaggtcc agcgcaacgt cgaagcgacc gctccgcagt tgccgggcga
     2581 gcgcgacgaa gcccgacgcc acggcccgca tggcgtccag gccgcgggcg cgacgccacc
     2641 cgcgcgtatc ggcgacgacg atctcgtcga tcgccgggtg gccgacgagc accaccgcct
     2701 cgcggcgctc gcacagccac gtgatgcgcg cgccggggag ccgcgcgcgc agtgtcgccg
     2761 ccaccggcag cgcgtggacg acgtcgccga tggaggacag cttgacgatg acgacattca
     2821 cgcccgcgat ccgccgggga agcgcgcgcg gatgcgcgcg atgaggtcgc gggtcgagtg
     2881 ccccttggga tcgccggcga tcgccacgtc cgcgccgacc gcccgggcgg tgtcccgctc
     2941 gggcaccgac tgcgccgtgt agtccgtgcc cttggcgtgc acgtgcggcc tgagccgcgc
     3001 gatcagaccg tcggcggtgt cctcgtcgaa gacgaccacg tggtccaccg cccgcagcgc
     3061 ggccagcagc tcggcgcgct cgccggcggg catcagcggg cgccccgcgc ccttgagacg
     3121 cgcgacggcg gcatcggagt tgatgccgac gaagagcacg tcgccgaggg cacgcgcgcc
     3181 gtccaggtag cgtacgtgac cgacgtggag gagatcgaag cagccgttgg cgaggatgat
     3241 ccgccggccg gccgcgcgga ggcgcccggc gagggctgcc gcctcgtcca cgctgagcgg
     3301 cgcagccccg ctcacgaggc ggcgccgagg gcctgggcca gctccacgcg cgagaccgtc
     3361 gcggtgccgc gcttcatcac cacgaggccg cccgccatgt tggcgagggt ggcggcctcc
     3421 accggcgacg ccccgctggc caacccgacg gtgaacgcac tgatcacggt gtcgcccgcc
     3481 ccggtgacgt cggcgatctc gtccgtaccg aagatcggga tgaacgtcgc cgccccctcg
     3541 cgctcgagca gcgcgagccc ctggctgccc cgggtgacca gcagcatgcc cgcgtcgagt
     3601 cgctcgagga gctggcggcc tgccttctcc accgcccgct cgtcgtccac gggggcgccc
     3661 gtcagctcgc ccagctcggc ctcgttgggc gtggccgcgg tcacgccgat gaagcgtggc
     3721 agctcgtagc ggctgtccac ggtgacgacg tttccgcgct tgcgggcgat ggcgcgcacc
     3781 cgctcgaaga cccgcggcgt cacggtgcca tagccgtagt ccgagacgac gatcgcatcg
     3841 gcccgctcgg cgagcgccgt gacccgcgcc agcaacgcgt cctccgtggt gggggccagc
     3901 tcgccggtgg gctcgcggtc catcctcacc acctgctggc gagttgcctg gtagccgccg
     3961 gccatgatcc gcgtcttcac gggtgtcgtg cggcccgcct cgacgacgag accgtccgtc
     4021 gggataccga cgccacggaa gagtgccatc agttcttcgc cggccacgtc gcggcccacg
     4081 acaccgatcg ggacgacgcg cgcgccgagg gcgtggacat tgtgggcggc gttcgcggcg
     4141 ccgccgagcc ggacgtcgcg gtcggtgaac ctcaggatga tcaccggcgc ctcgcgcgag
     4201 atccggccgg gcttgccgat gaggtactcg tcggtgatga ggtccccgac gaccgcgacg
     4261 acgcgcccgg cgaagaggtc gatggccgcg cgcagccgct cggatggcgt catcacgtcc
     4321 cccgccgcgc gagcgcccag tccacggcct cgagcaggtc gtcggccacg tggtcgggct
     4381 tcacggtgaa cgtctgacgc cggt
//
LOCUS       DATIAZ010000020         8589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1056811, whole genome shotgun sequence.
ACCESSION   DATIAZ010000020 DATIAZ010000000
VERSION     DATIAZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056811"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /gene="mraY"
                     /locus_tag="VLF19_00660"
     CDS             <1..153
                     /gene="mraY"
                     /locus_tag="VLF19_00660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851850.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSE91786.1"
                     /translation="FRLTGRRVFRMAPLHHHYELTGWAEPKIVVRFWIVSFALALLAL
                     TTLELR"
     gene            376..1746
                     /gene="murD"
                     /locus_tag="VLF19_00665"
     CDS             376..1746
                     /gene="murD"
                     /locus_tag="VLF19_00665"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSE91787.1"
                     /translation="MRGRAYLQTLTGRRVVVVGLARSGVAAARLLHAAGARVIATDAK
                     PLDALGPEASALALLGVRLHAGATAPAVEDAQLVVVSPGVPLDAPQLAPARARGVPVI
                     GELELAWRAMEADTLAITGTNGKTTTTALTGALLAEQARPVLVAGNIGTPLAASALTF
                     PADGLVVCEVSSFQLETIDVFHPRVAAVLNVTPDHLDRHGTLAAYADAKARVFANQTA
                     ADCAVLNADDPGSRALASRTRARVVWFSRRGALQHGVFVQDGWVVARLNGHAENICPV
                     DEIFLRGAHNLENVLAATACALWAGLAPEAIRRGVARFRGVAHRIELVRDLRGVHYYN
                     DSKGTNVDSTIKALESFAEPIVLIVGGVGKGQDFAPLAAAARGRVTHAVAIGQDGAKI
                     AAALEVAGIPVTAAASLEAALQTARALARPGDVVLLSPACASFDMFENYEHRGNVFKG
                     LVGGLV"
     gene            1746..2312
                     /locus_tag="VLF19_00670"
     CDS             1746..2312
                     /locus_tag="VLF19_00670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE91788.1"
                     /translation="MAPRMPAAARWPFAAERLLLAEPRVRPATDADLPFCWDMLHEVA
                     IAAPLLRGLPMADVLARPGVASHLTGWGRAGDIGLVAVGPDGAPLGAAWHRVLTPSER
                     GLGAPALAGVPELTMAVRRACRGVGVGSALLAGLAVRARQAGYERLAALVDPLNGAVK
                     FFRRHGFVEAGRHVGVDGVRLLLIGEIG"
     gene            2467..3564
                     /gene="ftsW"
                     /locus_tag="VLF19_00675"
     CDS             2467..3564
                     /gene="ftsW"
                     /locus_tag="VLF19_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HSE91789.1"
                     /translation="MPRKLQPDMWLLGVAVLLLSIGIVMVYSASAIVAADRFHDPYLF
                     LRKQLFWALLGCACLVAALRLDYRRLERLGWPIVIAAAVLLVLVLVPPFAQPINGSRR
                     WLRLGPVSVQPVELAKLALVIYLAAFISRRREGLADFRHGVLPPLAAAGGLAALVLVQ
                     PDLGNSLTLITVAFALLFLAGGQVRHLLLVCAAALPLLAMAVWLAPYRLRRITTFLDP
                     WSDPRGSGFQIIQSWLALGGGGWLGRGIGESKQKLFYLPESHTDFIFAVLGEELGFVG
                     AAAIIALFVVFVWRGLRVGMRAPDAFGAYLALGITLLIATEMLVNLGVVTGLLPTKGL
                     PLPFMSFGGSALVVTMLSTGVLLNISQHAHV"
     gene            3557..4957
                     /gene="murC"
                     /locus_tag="VLF19_00680"
     CDS             3557..4957
                     /gene="murC"
                     /locus_tag="VLF19_00680"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSE91790.1"
                     /translation="MFKRYRQIHFVGIGGSGMSGIAEILITLGYQVTGSDQRRSDAVE
                     RLEQLGAKVHIGHAAEHVEGAHVVVYSSAVSRDNVEVQAARQRGVPAIPRAEMLAELM
                     RLKYGIAVAGTHGKTTTTSMVGAVLAEGRLDPTIVVGGRVTGLGANARLGQGEFLVAE
                     ADESDGSFLKLAPTIAVVTTIDAEHLDHYGSLDAIREAFVAFVNKVPFYGAAVLCLDQ
                     PNIQMLLPRIEKRVITYGLEANADLVARRIHASGLTSRFEVSRRGTPLGECTLQVPGR
                     HNVLNALAAIGVGLDLDVPFATIQRALAGFAGVQRRFQVRGRAGDVMVVDDYGHHPAE
                     IRATLAAAKAGFDARVVCVFQPHRYTRTLHLGQEFLTAFNQADVLLVMDVYAAGEPPI
                     PGVSASALAEGIRAHGHREVTYVGADKARVVEHLVQITRPGDLVLTLGAGDVYQVGAE
                     LLLRLEHPSLGGRTTC"
     gene            4951..5829
                     /gene="murB"
                     /locus_tag="VLF19_00685"
     CDS             4951..5829
                     /gene="murB"
                     /locus_tag="VLF19_00685"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSE91791.1"
                     /translation="MLGEIRGEVRFKEPLSFHTSLRIGGAADIFIVPQDADDIRHALL
                     FAHREQLPLVVVGGGNNLLVGDRGVRGVVLKLEGCLGRSEFHGEEAVAGAGASLSALI
                     REGAALNLGGLESLVGIPATIGGALAMNAGTADGAIGDFVSAAYFLYPDGTLGEFKPN
                     SGAFTYRALHFPPGAVLVGCRLRLTRRPFAEIQREIKLRLKHKKSTQPLALASAGCVW
                     RNPPGELAAKLIEKVGLKGKRINGAEVSAKHANFIVNRGGAAATDVQALMEVTRDRVH
                     AHFGITLEPEIRTIGE"
     gene            5822..6691
                     /locus_tag="VLF19_00690"
     CDS             5822..6691
                     /locus_tag="VLF19_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HSE91792.1"
                     /translation="MSKVALVSPRGGAATLGDRGERTAFIAGQRVGRQAAARRRPAPG
                     RLSRGRRLLAGAAILAALVGPPAGVYWALTSPRFAVEDVEVRGASRVPVPRILAAAGV
                     APGTNLWRIDPTEAMARVQALPGIRRAELTRELPNRVTILVEERRPFTLVSSGRLHWL
                     DEEGWLLGEESQAVAPPVPVITGLSEDEVATMRTQPSEKARAAIGLIRALLRSGSPLA
                     AEISEIDMSRRDGPVLFTVDGIEVRLGTEDWEERLARLEGVLAQVTADESGVSAIDLR
                     FRDQVVLTKGGRG"
     gene            6688..7920
                     /gene="ftsA"
                     /locus_tag="VLF19_00695"
     CDS             6688..7920
                     /gene="ftsA"
                     /locus_tag="VLF19_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSE91793.1"
                     /translation="MARARVRDLVVGLDVGTTKTCCVIAEPASGGGLDVVGVGAAPSR
                     GLRKGVVVNIESTVEAIKHAVAEAEQMAGAEVSGVYAGVAGGHIRGVNSRGVVAVSGK
                     DREVSAADVERAIDAARAINLPQDREIVHVLPQTFVVDEQDGIREPVGMTGVRLEVEV
                     HVVTGAITSVQNVVRSVNRAGLTVHDIVLEPLASAEAVLYPEERELGVVLIDIGGGTT
                     DVAFFRDGAIWHTAILPLGGDHVTNDIAVGLRAPVAEAEDLKRRYGCALTALVSPEET
                     VEVASVGGRKPRQLSRQILSEIVQPRMEEILTLVARELAKAGVQDAATAGVVVTGGTA
                     IMEGVPELAEAVFDLPVRRGVPETIGGLADVVKSPVYATGVGLALYGARAAESAPSSS
                     KDQSSLVRRLASWFGEIF"
     gene            8102..>8589
                     /locus_tag="VLF19_00700"
     CDS             8102..>8589
                     /locus_tag="VLF19_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSE91794.1"
                     /translation="MEKERGRRPLFALDEAPQAAKIKVIGLGGGGSNAVSRMIAAQSI
                     GVDFIVANTDAQALRASPAPVRIQLGAKLTQGLGAGSNPQIGREAALEDPEQITRLLQ
                     GADMVFITAGLGGGTGTGAAPVVASLAKDLGILTVAVVTKPFHFEGRRRMLQAEAGME
                     ALR"
BASE COUNT         1085 a         3113 c         3164 g         1227 t
ORIGIN      
        1 ttcaggctca ccggacgccg cgtcttccgc atggctccgc tccaccacca ctacgagctg
       61 accggctggg cggagcccaa gatcgtcgtg cgcttctgga tcgtctcgtt cgcgctggcg
      121 ctgctggcgt tgacgaccct ggagctgcga tgagcagccg cgtgacccga gcgctgcgtg
      181 ggcgcgcgct ggcttcggcg gcccaacctc ttctggaagg gcccggcatg accccggcgg
      241 cgcgggttcg agcgccgcga tgagggagac cacggcatga ctcgacagat gctcggtcga
      301 gcgccggcgg tgccggcgca acctctccgg ggggaggctt cggaggggga cgtcgaggct
      361 ccctcggaag aagtcatgcg gggacgagcg tacctgcaga cgctgacagg ccgccgggtg
      421 gtcgtcgtcg ggctcgcgcg cagcggcgtg gccgcggcgc gcctgctcca cgccgccggt
      481 gcccgcgtga ttgccacgga cgccaagccg ctcgacgcgc tcgggccgga ggcgagcgct
      541 ctggccctgc tgggcgtgcg gctgcacgcc ggcgcgacgg ccccggccgt cgaggacgcg
      601 cagctcgtcg tggtcagccc gggcgtcccg ctcgacgcgc cgcagctcgc cccggcccgc
      661 gctcgcggcg tgccggtgat cggcgagctc gagctcgcct ggcgggcgat ggaggccgac
      721 accctggcca tcaccggcac caacggcaag acgaccacga ccgcgctcac gggcgcgctc
      781 ctggccgagc aggcccggcc cgtcctcgtg gccggcaaca tcggcacgcc gctcgcggcc
      841 tccgcgctca cctttccggc cgacggcctc gtcgtgtgcg aggtatcgag cttccagctc
      901 gagaccatcg acgtcttcca cccccgggtg gcggccgttc tcaacgtcac gccggaccac
      961 ctggaccggc acggcacgct cgcggcgtac gcggacgcca aggcgcgcgt cttcgccaac
     1021 cagacggcgg cggactgcgc ggtgctcaac gccgacgacc cgggcagccg cgcgctggcg
     1081 tcccggacgc gggcacgcgt ggtgtggttc agccggcgcg gcgcgctcca gcacggcgtg
     1141 ttcgtgcagg acggctgggt ggtggcgcgg ctcaacggcc acgccgagaa catctgcccg
     1201 gtggacgaga tcttcctgcg cggcgcccac aacctcgaga acgtgctcgc ggcgaccgcc
     1261 tgcgcgctgt gggccggcct cgcgcccgag gccatccgcc gcggcgtcgc gcggtttcgc
     1321 ggcgtggccc atcgcatcga gctcgtgcgc gacctccggg gcgtccacta ctacaacgac
     1381 tcgaagggca cgaacgtcga ctccacgatc aaggcactcg agagcttcgc ggagccgatc
     1441 gtcctcatcg tcggcggtgt cggcaagggg caggacttcg ccccgctggc cgcggccgcg
     1501 cgggggcggg tgacccacgc cgtggcgatc gggcaggacg gggcgaagat cgcggcggcg
     1561 ctcgaggtcg ccggcatccc ggtgaccgcg gccgcctcgc tcgaggccgc gctgcagacg
     1621 gcccgcgccc tcgcgcggcc cggcgacgtc gtcctgttgt cgccggcgtg cgcctccttc
     1681 gacatgttcg agaactacga gcatcgcggc aacgtgttca agggcctggt gggagggctc
     1741 gtctgatggc gccccgcatg ccggcggcgg cacggtggcc cttcgccgcc gagcgtctcc
     1801 tcctggccga gccgcgggtg cgtccggcga ccgacgccga tctgcccttc tgctgggaca
     1861 tgctccacga ggtcgccatc gcggcgccgt tgctccgcgg cctgccgatg gcggacgtgc
     1921 tggcgcgtcc cggcgtcgcc tcacacctga cgggctgggg acgcgcgggc gacatcggcc
     1981 tcgtggccgt cggccccgac ggcgcgccgc tgggcgccgc ctggcatcgc gtgctcacgc
     2041 cgtcggagcg cgggctcggc gcgccggcgc tcgccggcgt gccggagctc accatggccg
     2101 tgcgccgcgc ctgccggggt gtcggtgtcg gctcggcgct gctggccggc ctcgccgtcc
     2161 gggcgcgcca ggcggggtac gagcgcctgg cggcgctcgt cgatccgctc aacggcgccg
     2221 tcaagttctt ccggcgccac ggcttcgtcg aggccggccg ccacgtcggc gtggacggcg
     2281 tgcgcttgct gctgatcgga gagatcggct agatgcgcat ccgggctcgg ctcggttcga
     2341 gcgcggactt cgcccgcgca tcctgcctcg agcgcgggct ttgcccgcgc cctgcagatc
     2401 ctgaagcccg tgttccacgc tggggggctg tcgaggggag cggcgcgctc cccccgacta
     2461 cgtaggatgc cgcgcaagct ccagcccgac atgtggctgc tcggcgtggc cgtcctgctg
     2521 ctgagcatcg gcatcgtcat ggtgtactcg gcgagcgcca tcgtcgccgc cgaccgcttc
     2581 cacgacccgt atctcttcct gcgcaagcag ctcttctggg cgctgctcgg ctgcgcgtgc
     2641 ctggtcgccg ccctccgcct ggactaccgg cgtctggaga ggctcggctg gcccatcgtg
     2701 atcgcggcgg cggtactgct ggtgctggta ctcgtgccgc cgttcgccca gccgatcaac
     2761 ggcagccggc gctggctccg actcgggccc gtctcggtcc agccggtgga gctcgccaag
     2821 ctcgcgctcg tgatctatct ggccgccttc atctcacgcc ggcgggaggg gctcgccgac
     2881 ttccgccacg gcgtcctgcc gccgctcgcg gcggccggcg ggctggccgc gctcgtgctg
     2941 gtacagcccg atctcggcaa cagcctgacg ctcatcaccg tcgcgttcgc cctgctgttc
     3001 ctggccggcg gccaggtgcg ccatctgctg ctggtctgcg cggcggcgct gccgctcctg
     3061 gcgatggccg tgtggctggc gccctaccgg ctgcgccgga tcacgacgtt cctcgacccg
     3121 tggagcgacc cgcgcggcag cggcttccag atcatccagt cgtggctggc cctgggcggg
     3181 ggcgggtggc tgggccgcgg catcggcgag tcgaagcaga agcttttcta cctgcccgag
     3241 tcgcacaccg acttcatctt cgccgtgctc ggcgaggagc tgggcttcgt cggagcggcc
     3301 gcgatcatcg cgctcttcgt ggtcttcgtc tggcgcggcc tgcgcgtcgg gatgcgggcg
     3361 ccggacgcct tcggcgcgta cctggcgctc ggcatcacgc tgctgatcgc caccgagatg
     3421 ctcgtcaacc tcggcgtcgt cacggggctc ctgcccacga agggcctgcc cctgccgttc
     3481 atgtcgttcg gcggctcggc gctggtcgtg accatgctgt cgaccggggt gctcttgaac
     3541 atctcccagc acgcccatgt ttaagcgcta ccggcagatc cacttcgtgg ggatcggcgg
     3601 cagcggcatg tccggcatcg ccgagatcct catcaccctc ggctaccagg tgaccggctc
     3661 cgaccagcgg cgcagcgacg cggtcgagcg cctcgagcag ctcggcgcca aggtccacat
     3721 cggccacgcc gccgagcacg tcgagggcgc ccacgtcgtc gtctactcct cggcggtgtc
     3781 ccgcgacaac gtcgaggtgc aggccgcgcg ccagcgcggg gtgccggcga tcccgcgggc
     3841 ggagatgctc gccgagctga tgcggctcaa gtacggcatc gccgtggccg gcacccacgg
     3901 caagacgacg accacgtcga tggtcggcgc cgtcctggcc gagggccgcc tcgacccgac
     3961 catcgtggtc ggcgggcgcg tgaccgggct gggcgccaac gcccggctcg ggcagggcga
     4021 gttcctggtc gcggaggccg acgagtccga cggctcgttc ctgaagctcg cgccgacgat
     4081 cgccgtggtg acgacgatcg acgccgagca tctcgaccac tacggctcgc tcgacgccat
     4141 ccgcgaggcc ttcgtcgcct tcgtcaacaa ggtgccgttc tacggcgccg cggtcctctg
     4201 cctcgaccag ccgaacatcc agatgctgct gccgcggatc gagaagcgcg tcatcaccta
     4261 cggcctggag gccaacgccg acctcgtcgc ccgccggatc cacgcgagcg ggttgacgag
     4321 tcgcttcgag gtctcccggc gcggcacgcc gctcggcgag tgcaccctgc aggtgcccgg
     4381 gcgccacaac gtgctgaacg cactcgccgc gatcggcgtc gggctcgacc tcgacgtccc
     4441 gttcgccacg atccagcggg cgctggccgg ctttgcgggt gtccagcgcc gcttccaggt
     4501 gcggggacgg gccggggacg tgatggtcgt cgacgactac ggccaccacc cggccgagat
     4561 ccgggccacg ctggctgccg ccaaggccgg cttcgacgcc agggtcgtgt gcgtcttcca
     4621 gccccaccgc tacacgcgca cgctccacct cggccaggag ttcctcaccg cgttcaacca
     4681 ggccgacgtc ctgctcgtca tggacgtcta cgcggccggc gagcctccga tccccggcgt
     4741 gagcgccagc gcgctcgccg agggcatccg ggcgcacggc caccgcgagg tcacctacgt
     4801 cggtgcggac aaggcgcggg tcgtggagca cctcgtgcag atcacgcgcc ccggggacct
     4861 cgtgctcacg ctcggggcgg gcgacgtcta ccaggtcggt gccgagctct tgctccgact
     4921 cgagcacccc tcgctcggag ggaggaccac atgctagggg agatccgggg ggaggtccgg
     4981 ttcaaggaac cgctgagctt ccacacctca ctgcgcatcg gaggcgccgc cgacatcttc
     5041 atcgttcctc aggacgcgga tgacatccgg cacgcgctgc tgttcgcgca ccgcgagcag
     5101 ctgccgctgg tcgtcgtcgg cggcggcaac aacctgctgg tgggcgaccg cggcgtccgc
     5161 ggcgtcgtgc tcaagctcga ggggtgcctc gggcggagcg agttccacgg cgaggaagcc
     5221 gtggctggcg cgggcgcgag cctctcggcg ctgatccgcg agggggccgc gctgaacctc
     5281 ggcgggctcg agtccctggt cggcatcccg gcgaccatcg gcggcgccct ggccatgaac
     5341 gcgggcaccg cggacggggc gatcggcgat ttcgtctccg ccgcgtactt cctctacccg
     5401 gatggcacgc tcggcgagtt caagccgaac tccggggcct tcacgtaccg cgcgttgcac
     5461 tttccgcccg gcgccgtcct cgtcggctgt cggctccggc tgacccgccg gccgttcgcc
     5521 gagatccagc gcgagatcaa gctgcggctc aagcacaaga aatccacgca gccgctggcg
     5581 ctggcctcgg cgggctgcgt gtggaggaac ccgccgggcg agctggcggc gaagctcatc
     5641 gagaaggtgg ggctcaaggg caagcggatc aacggggcgg aggtgtccgc caagcacgcc
     5701 aacttcatcg tcaaccgggg cggcgccgcg gccacggacg tccaggcgct gatggaggtg
     5761 acgcgcgacc gcgtccacgc gcacttcggc atcactctcg agcccgagat caggacgatc
     5821 ggtgagtaag gtggcgctgg tctccccgcg cgggggcgcg gcgacgctcg gcgaccgcgg
     5881 cgagcgcacg gcgttcatcg cgggccagcg agtcggccgg caggcggcgg cgcgccgacg
     5941 tccggcgccg ggccgcctct cgcgcggccg ccggctcctc gccggcgcgg cgatcctcgc
     6001 agcgctcgtc ggcccccccg ccggcgtcta ctgggcactc acctcgccgc gcttcgccgt
     6061 cgaggacgtc gaggtgcgcg gcgcctcgcg cgtgcccgtg ccgcgcatcc tggccgccgc
     6121 cggcgtcgcg ccgggcacga acctgtggcg gatcgacccg accgaagcga tggcgcgggt
     6181 gcaggcgctg cccgggatcc ggcgtgcgga gctcacgcgg gagctgccga accgcgtgac
     6241 gatcctggtg gaggagcggc ggccgttcac gctcgtctcc agcggccgcc tgcactggct
     6301 ggacgaggag ggctggctgc tgggcgagga gtcccaggcc gtggcgccgc cggtgccggt
     6361 catcaccggg ttgtcggagg acgaggtggc gacgatgcgg acccagccga gcgagaaggc
     6421 gcgcgcggcg atcggcctga ttcgggcgct gctgcgcagc ggcagcccgc tcgccgccga
     6481 gatctcggag atcgacatga gccggcggga cggtcccgtg ctcttcaccg tcgacggcat
     6541 cgaggtgcgg ctcgggaccg aggactggga ggagcggctg gcgcggctgg agggcgtgct
     6601 cgcacaggtc accgccgacg agagcggcgt gagcgcgatc gacctccgct tccgcgacca
     6661 ggtggtcctc acgaaaggag gccggggata gcgcgcgcca gggtcaggga cctcgtcgtc
     6721 gggctcgacg tgggcaccac gaagacctgc tgtgtcatcg ccgagcccgc gagcggcggt
     6781 ggcctcgacg tggtcggcgt cggcgcggcg ccgtcgcgcg ggctgcgcaa gggcgtcgtc
     6841 gtcaacatcg agtccacggt cgaggccatc aagcacgccg tggccgaggc cgagcagatg
     6901 gccggcgccg aggtgtccgg cgtgtacgcg ggcgtggccg gcggtcacat ccgcggcgtc
     6961 aacagccggg gcgtcgtggc ggtgtccggc aaggaccgcg aggtgagcgc ggccgacgtc
     7021 gagcgcgcga tcgacgccgc gcgggcgatc aacctgccgc aggaccgcga gatcgtgcac
     7081 gtcctgcctc agaccttcgt ggtcgacgag caggacggca tccgggagcc cgtgggcatg
     7141 acgggcgtgc ggctcgaggt cgaggtgcac gtggtgaccg gcgccatcac gtcggtgcag
     7201 aacgtggtcc gttcggtgaa ccgcgccggc ctgaccgtcc acgacatcgt gctggagccg
     7261 ctcgcctccg ccgaggccgt gctctacccg gaggagcgcg agctgggcgt ggtgctgatc
     7321 gacatcggcg gcggcacgac cgacgtcgcg ttcttccggg acggcgccat ctggcacacg
     7381 gcgatcctgc ccctcggtgg tgaccacgtc accaacgaca tcgccgtggg cctgcgcgcg
     7441 ccggtggccg aggcggagga cctcaagcgc cgctacggct gcgccctcac cgcgctcgtg
     7501 tcgcccgagg agaccgtcga ggtggcctcg gtcggcgggc gcaagccgcg gcagctctcg
     7561 cgccagatcc tctccgagat cgtgcagccc cgcatggagg agatcctcac gctggtggcc
     7621 cgtgagctgg cgaaggcggg cgtccaggat gcggcgacgg cgggcgtggt cgtgaccggc
     7681 ggcaccgcga tcatggaggg cgtgccggag ctggccgagg cggtgttcga cctgccggtg
     7741 cggcggggcg tgcccgagac gatcggcggc ctcgccgacg tggtgaagag cccggtgtac
     7801 gccaccggcg tggggctcgc gctctacggc gcgcgcgccg cagagagcgc gccgtcgtcc
     7861 tcgaaggacc agagcagtct cgtccggcgg ctggcgagct ggttcggtga aatcttctga
     7921 catgggggcc ccgacatggc ccccaagccc ccagggtggc tcggcgcgcc ccgccgaagc
     7981 cgcggcgcgc ctcggtcatc ctggacggtc atcgtctcgc aatcagccgg ggctgcccgg
     8041 cgtgacggtg gagacgctcg agcgccggcc ggaccggcgc aatcccgggg ggaggtgctg
     8101 gatggagaag gaacgcggca gacgcccgct gttcgcgctg gacgaggctc cgcaggcggc
     8161 gaagatcaag gtcatcgggc ttggcggcgg tggcagcaac gcggtgagcc gcatgatcgc
     8221 ggcgcagtcc atcggcgtcg acttcatcgt cgccaacacc gacgcccagg ccctgcgcgc
     8281 gtcgccggca ccggtgagga tccagctcgg cgcgaagctc acccagggcc tcggtgcggg
     8341 ctccaacccc cagatcggac gggaggccgc gctcgaggac cccgagcaga tcacgcgcct
     8401 gctccagggc gccgacatgg tgttcatcac cgcgggcctc ggcggcggca ccggaacggg
     8461 cgccgcgccc gtcgtcgcct cgctcgccaa ggatctcggc atcctgaccg tcgccgtggt
     8521 cacgaaaccc ttccacttcg agggacggcg gcgcatgctc caggccgagg ccggcatgga
     8581 ggcgctgcg
//
LOCUS       DATIAZ010000021         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_136187, whole genome shotgun sequence.
ACCESSION   DATIAZ010000021 DATIAZ010000000
VERSION     DATIAZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136187"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VLF19_00705"
     CDS             <1..216
                     /locus_tag="VLF19_00705"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HSE91795.1"
                     /translation="YATADGQVTRAANPNGSLENIAGIVNETGTVLGLMPHPERAAEP
                     AMGGTDGLAILQSLLGSLVEDGTFLKR"
     gene            230..>2130
                     /gene="purL"
                     /locus_tag="VLF19_00710"
     CDS             230..>2130
                     /gene="purL"
                     /locus_tag="VLF19_00710"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HSE91796.1"
                     /translation="MTGSEPKATPDLARELGLTADEYDRIIQRLGREPTWTELGLFSA
                     LWSEHCAYKHSRVFLARLPTTGPRVLQGPGENAGALEIGDGWAVVFKIESHNHPSFIE
                     PLQGAATGVGGILRDIFTMGARPIAILDSLRFGDPDDPKMRRLIEGVVSGISWYGNCF
                     GCPTVGGEIAFAPEYAGNPLVNVMCVGLVRADRIFRARAEGVGNPVFYVGNKTGRDGI
                     HGATMASATFDERAEERRPTVQVGDPFTEKLLLEACLEAMRTGAIVGIQDMGAAGLAC
                     CTSEMPARSGTGMDIEVSRVPQRETQMTPYEILLSESQERMLLVVARGREDEIRRVFA
                     KWELDAVEIGHVTGDGELTVRDHGEVVARVAVRALAEAPKYEKPTDPPPGLAALRAFD
                     PMTLSAPDDCGEALASLLGSPTIACKEWAYRQYDHQVGINTLVLPGSDAAVLRVKGTR
                     LALAVATDGNGRQVFLDPRRGAAMAVAEAARNVSCAGGRPLGVTDCLNFGSPERPEIL
                     WQFAEAIEGIAEACRALDLPVVGGNVSFYNETVQARGVTAPSDSPGGHAILPTPIVGV
                     VGLLEDASRVATQWFKGPGHRIALLGPDAVSLGGSEYLWIRHRRLAGALGPVDLGTER
                     RVQAAIQAAV"
BASE COUNT          314 a          731 c          781 g          304 t
ORIGIN      
        1 tacgccacgg cggacggaca ggtcacgcgg gcggcgaatc cgaacggatc gctggagaac
       61 atcgcgggca tcgtgaacga gacgggaacc gtcctcgggc tgatgccgca tcccgagcgg
      121 gccgccgagc cagcgatggg aggcaccgac gggctcgcca tcttacagtc gctcctcggc
      181 agcctcgtcg aggacggcac gttcctcaag cggtagcgct ggagggggat tgacgggttc
      241 ggagccgaaa gccacaccgg acctcgcccg cgaactcgga ctcaccgccg acgaatacga
      301 ccggatcatc cagcgtctcg gccgggagcc cacctggacg gagctggggc tgttctccgc
      361 cctctggtcc gagcactgtg cctacaagca ctcccgcgtg ttcctcgcac ggttgcccac
      421 gaccgggccg cgggtgttgc aggggccggg cgagaacgcc ggcgcgctcg agatcggcga
      481 tggctgggcc gtcgtcttca agatcgagag tcacaaccac ccctcgttca tcgagccact
      541 gcaaggcgcg gccaccggag tcggcggcat cctgcgcgac atcttcacga tgggcgcccg
      601 tcccatcgcc atcctcgact cgctgcgctt cggcgacccc gacgacccga agatgcgccg
      661 gctgatcgaa ggcgtggtct ccggcatctc gtggtacggc aactgcttcg gctgcccgac
      721 cgtcgggggc gagatcgcct tcgccccgga atacgcgggc aacccgctcg tgaacgtcat
      781 gtgcgtcggc ctcgtccggg ccgaccggat cttccgcgcg cgggccgagg gcgtgggcaa
      841 cccggtcttc tacgtgggta acaagaccgg gcgtgacggc atccacggcg ccaccatggc
      901 ctcggcgacg ttcgacgagc gcgccgagga gcgccggccg acggtgcagg tcggcgaccc
      961 tttcaccgag aagctcctgc tcgaggcgtg cctggaggcc atgcggacgg gcgcgatcgt
     1021 cggcatccag gacatgggcg cagccgggct ggcgtgctgc acgtcagaga tgccggcgcg
     1081 cagcgggacc ggcatggaca tcgaggtctc gcgagtgccg cagcgcgaga cgcagatgac
     1141 cccctacgag atcctgctct cggagtcgca ggagcggatg ctgctcgtgg tcgcgcgtgg
     1201 ccgcgaggac gagatccggc gcgtcttcgc caagtgggag ctcgacgccg tggagatcgg
     1261 gcacgtgacc ggcgacggcg agctgacggt tcgcgatcac ggcgaggtcg tcgcgcgggt
     1321 ggccgttcgg gcgctcgccg aggcgccgaa gtacgagaag ccgaccgacc cgccgccggg
     1381 gctcgccgcg ctccgcgcgt tcgacccgat gacgctgtcc gcgccggatg actgcggcga
     1441 ggcgctcgcg agcctgctcg ggtcgccgac gatcgcctgc aaggagtggg cataccgaca
     1501 atacgaccac caggtgggca tcaacacgct cgtgctgccg ggctcggacg ccgccgtgct
     1561 tcgcgtgaag ggtacgcgcc tggcgctcgc ggtcgccacc gacggcaacg gacgccaggt
     1621 cttcctcgac ccgcggcggg gcgccgccat ggctgtcgcc gaggcggcgc gcaacgtatc
     1681 gtgcgcgggc gggcggccgc ttggcgtcac cgactgcctg aacttcgggt ccccagagcg
     1741 tccggagatc ttgtggcagt tcgccgaggc catcgagggc atcgccgagg cgtgccgcgc
     1801 gctggacctg ccggtagtgg gcggcaacgt ttccttctac aatgagaccg tgcaggcgag
     1861 gggggttacg gccccctcgg actcccccgg ggggcacgcg atcctgccta cgccgatcgt
     1921 gggcgtggtc gggctgctcg aggacgcctc gcgggtggcg acgcagtggt tcaagggacc
     1981 ggggcaccgg atcgcgctcc tcggccccga tgcggtgagc ctgggtggca gcgagtatct
     2041 ctggatccgc catcgccggc tcgccggggc gctcgggccg gtcgatctcg gcaccgagcg
     2101 gcgcgtgcag gccgcgatcc aggcggcggt
//
LOCUS       DATIAZ010000022         5657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_925365, whole genome shotgun sequence.
ACCESSION   DATIAZ010000022 DATIAZ010000000
VERSION     DATIAZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925365"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VLF19_00715"
     CDS             <1..1173
                     /locus_tag="VLF19_00715"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSE91797.1"
                     /translation="RYRYHVPAELEGTLHDRYVATLDRAFDVYGAWLPRLRDFYAERF
                     PRAESDSEAVHRMTIRAKALDTLRGLLPAATVSNVGIYGTGQGYEQLLLRMRAHPLAE
                     VRDYAGLMLVELRKVIPAFLRRVDVPDRGGAWSAYLAQTRAGAEATAARALAGVGPEP
                     RGEVTLTDFDPEGEVKVVAAALYAASELPDDQLLERARKMTHDERLAVLDAYVGQRGN
                     RRHKPGRAFERTSYRFDVLADYGAFRDLQRHRLLTLEWQRLTPRHGWVVPAAIDEAGA
                     GTDWARVMADSAALHDTIAAAGLPHVASYAVSMAYRVRFYMEMNAREAMHLVELRSAR
                     QGHPAYRRISQTMHRLIAERAGHRAIAAAMTFADHSEVALERLEAERAAARRRARS"
     gene            1338..1937
                     /locus_tag="VLF19_00720"
     CDS             1338..1937
                     /locus_tag="VLF19_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HSE91798.1"
                     /translation="MAPVRREQIVRATIRCLARSGYAGLTMKKVSREAGVSQGILHYY
                     FPGKRAILVAALEAVMADIDRRVAAAQARSGRDPRARLRALVRTSLGTAVDAREVWVV
                     FVEFWGETMHDRRLREVNAALYARMRRLIGRLVAEGVRAGHWRRVDPATAGAVILGLL
                     DGVALQLTFAPGVFGVAEAVRFCEEAVERYLAEGVRHGR"
     gene            1934..2515
                     /locus_tag="VLF19_00725"
     CDS             1934..2515
                     /locus_tag="VLF19_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91799.1"
                     /translation="MKEIHVDQEAVFKQKARDYLRTRGTLAASDTIREQVAAAFGTLD
                     TVLAGVTAERAALATIAGEWAVQEIVDHLVETHRPGLDELRCLCAGRRPPGEPIPAGL
                     QSKAPLLRPWTWLLAELRRVHGDIVAVLGSVPPDFETAARAPLVMVANVPSGDGQIRA
                     VHWVEELDWKAYSIVWRLHALDHLAQIRKVLAA"
     gene            2512..2934
                     /locus_tag="VLF19_00730"
     CDS             2512..2934
                     /locus_tag="VLF19_00730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSE91800.1"
                     /translation="MSLAATAPELAALLTEIPVAREHGFRLVEVADGACTVEVPFAPA
                     LERPGGVVAGPAFMAAADVTMWFAILTRLGTADESVTAHLDTTFVAAARREGFRCTAR
                     VLKLGSRLIHGVAECVAADGRLLTHHTITYARAGPPSF"
     gene            complement(2900..3490)
                     /locus_tag="VLF19_00735"
     CDS             complement(2900..3490)
                     /locus_tag="VLF19_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HSE91801.1"
                     /translation="MKRLGAVVALSGLAAACATVSGGRPETARAELKSPGGQTVGTAT
                     FTEVSGGVRVVVEVRGIPAGARAVHVHEVGRCEPPGFTSAGGHFNPGKRQHGILNPEG
                     PHAGDLPNMTVAPDGTGRLETTTDRFTLRAGPTSLFDADGSALVVHAAPDDFRTDPTG
                     NSGGRAACGVLAKDDRLTWGPRPHVPRSWAGPPARR"
     gene            complement(3487..4680)
                     /locus_tag="VLF19_00740"
     CDS             complement(3487..4680)
                     /locus_tag="VLF19_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HSE91802.1"
                     /translation="MTTDDLKAGIAAAADGLANELEALSHRIHDHPELGFGEEKAQAW
                     LTDFLEKHGARVERGVGGLPTAFRATIPGTAPGPTIAILCEYDALPGIGHACGHNVIA
                     TAGAGAGAALARALGTLPFPGCVQIIGTPAEEGGAGKVKLMEAGVFRDVDAAMMVHGR
                     CGTQVWRPSLGIIKVKVEYFGKAAHASSWPWRGVNALNAVIQLFVAVDAMRQQLRPDA
                     RVHGVIRNGGAQPNIIPEYTSAEFYLRALDREYCHGLLRRFESVAQGAAVATGCQVRI
                     GQDGPMHEPLRPNATMAGLFRKNLERIDFPEDPDDGEAGFGSTDCGNVSQAIPTIHPY
                     VRISPDGVPGHSREFAEWARSPIARVGMLAGAKAMALTALDLLAAPQALQSAKHDFAR
                     GGHSA"
     gene            complement(4677..>5657)
                     /locus_tag="VLF19_00745"
     CDS             complement(4677..>5657)
                     /locus_tag="VLF19_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSE91803.1"
                     /translation="AFRATLDSGDGPTIAIMCEYDALPSIGHACGHNAIAAAGAGAGA
                     GLAALRDRLPKGRIHVIGTPAEEGGGGKIRLIRGGVFEGVDAAMMCHGWDRWIAHQDL
                     LGIVRVGFEFSGKAAHAAADPWEGVNALDAVIQTFNNVSMLRQQTRPDARIHGIITSG
                     GAAPNIIPEYAAATFYVRSASLDYMWALQKRVIACAEGAARATGSSLKTTERDDAYEP
                     MRRNETLLEAWRQNLRRLGLTEVPEVKDRLGSSDVGNVSQVVPTIQPLMKIAPDGTPI
                     HSRAFEAAAVSPLAREGLLAAAKAMAMTTLDLLSDPTLVARAKQEMAGAR"
BASE COUNT          736 a         2047 c         2069 g          805 t
ORIGIN      
        1 cgctaccgct atcacgtacc cgccgaactg gagggcacgc tgcacgaccg gtacgtcgcg
       61 accctcgatc gggccttcga tgtgtacggc gcgtggctgc cgcggctgcg cgacttctac
      121 gccgagcgct ttccccgagc cgagagcgat tccgaggccg tccaccggat gacgatccgc
      181 gcgaaggcgc tggacacgct ccgaggcctg ctgccggcgg ccaccgtgtc caacgtgggc
      241 atctacggca ccgggcaggg atacgagcag ctcctgctcc gtatgcgggc gcaccccctc
      301 gccgaggtgc gggactacgc gggcctcatg ctggtcgagc tacgcaaggt gattcccgcg
      361 ttcctgcgcc gcgtggacgt gcccgaccgt ggcggggcgt ggtccgccta cctcgcgcag
      421 actcgggcgg gcgccgaggc tacggcagcg cgggcgctcg cgggggttgg gccagagccg
      481 cgcggcgagg tcaccctgac cgacttcgat cccgagggcg aggtgaaggt ggtggcggcc
      541 gcgctctacg cggcgtccga gctccccgac gaccagcttc tcgaacgcgc ccggaagatg
      601 acgcacgacg agcgcctcgc cgtcctggac gcctacgtgg gccagcgcgg caaccgccgc
      661 cacaagcctg gccgggcctt cgagcgcacg tcgtaccgct tcgacgtgct cgcggactac
      721 ggcgcgttcc gcgatctgca gcgccaccgg ctgctcacgc tggagtggca gcggctcacg
      781 ccccgccacg gctgggtggt cccggccgcg atcgacgagg cgggcgctgg caccgactgg
      841 gcgcgcgtca tggcggactc ggccgcgctc cacgacacga tcgctgcggc ggggctgccg
      901 cacgtcgcct cgtacgcggt gtcgatggcg tatcgcgtcc gcttctacat ggagatgaac
      961 gcgcgcgagg cgatgcacct cgtcgagctc cgcagcgcgc gccagggaca ccccgcctat
     1021 cgccgcatca gccagaccat gcaccggctc atcgccgagc gcgcgggcca ccgtgcgatc
     1081 gccgcggcca tgaccttcgc cgaccattcc gaggtggcgc tggagcgtct cgaggccgaa
     1141 cgggctgccg cgcgccggcg cgcccgatcg tgaccgcagg gcaacggccc tcgctcgggc
     1201 ccgcgcgccc gagtcgcctc ccgccccgcc cgcgctgatc gtccgcgacg gcttgacgac
     1261 ggcgcgcggt gccaacataa ttggtcgtcc agccaaaaaa ggagggaggc ggcgagccac
     1321 tgagcccgaa ggtcggcatg gcgccggtgc gccgcgagca gatcgtgcgg gcgacgatcc
     1381 gatgtctcgc ccgcagcggc tacgccggcc tcacgatgaa gaaggtcagt cgcgaggccg
     1441 gcgtgagcca ggggatcctg cactactact tcccgggcaa gcgcgcgatc ctcgtcgcgg
     1501 cgctcgaggc cgtcatggcc gacatcgacc gacgcgtcgc cgcggctcag gccaggagcg
     1561 gccgcgaccc gcgcgcccgg ctccgggcgc tggtgcggac ctcgctggga acggcggtcg
     1621 acgcgcgcga ggtgtgggtg gtgttcgtcg agttctgggg cgagacgatg cacgaccgcc
     1681 ggctccgcga ggtcaatgcc gcgctctacg cgcgcatgcg gcgcctcatc gggcggctgg
     1741 tcgccgaggg cgtgcgggcc gggcactggc ggcgggtcga tcccgccacg gcgggagcgg
     1801 tgatcctcgg gctgctcgac ggcgtcgcgc tgcagctgac gttcgccccc ggtgtcttcg
     1861 gagtggcgga ggccgtacgg ttctgtgaag aggcggtcga gcggtacctg gccgaaggcg
     1921 tgcgccacgg ccgatgaagg agatccacgt ggaccaggaa gcggttttca agcagaaggc
     1981 gcgggactac ctccgcacgc gcgggacgct ggcggcgtcg gacacgatcc gcgagcaggt
     2041 ggccgccgcg ttcgggacgc tggacacggt gctggccggg gtgacggccg agcgggccgc
     2101 gctggccacg atcgccggtg agtgggcggt gcaggagatc gtggaccacc tcgtcgagac
     2161 gcaccgcccg gggctcgacg agctgcgctg tctgtgcgcg ggccggcgcc cacccggcga
     2221 gccgatcccc gccggactcc agtccaaggc gccgctgctg cggccctgga cgtggctgct
     2281 ggccgagctg cgtcgcgtgc acggcgacat cgtcgccgtc ctcggctccg tcccgcccga
     2341 cttcgagacc gccgcccgcg cgccgctggt catggtggcc aacgtgccgt ccggcgacgg
     2401 acagatccgg gcggtacact gggtcgagga gctggactgg aaagcctaca gcatcgtctg
     2461 gcgcctgcac gcgctcgatc acctcgcgca gatccgcaag gtcctcgccg cgtgagcctg
     2521 gcggcgaccg caccggagct ggcggcgctc ctgaccgaga tccccgtcgc ccgcgagcac
     2581 ggcttccggc tcgtggaggt cgccgacggc gcctgtacgg tggaggtgcc cttcgcgccg
     2641 gccctcgagc ggcccggcgg cgtcgtggcg ggccccgcgt tcatggccgc ggccgacgtc
     2701 acgatgtggt tcgcgatcct gactcggctc ggcacggccg acgagtccgt gaccgcgcac
     2761 ctggacacga cgttcgtcgc cgcggcgcgg cgcgaaggct tccgctgcac cgcgcgggtg
     2821 ctcaagctcg ggagccggct gatccacggc gtggccgagt gcgtggccgc cgacggccgc
     2881 ctcctcacgc accacacgat cacctacgcg cgggcgggcc cgcccagctt ctaggaacat
     2941 gggggcgggg tccccatgtc agtcggtcgt ccttggcgag cacgccacag gcggcgcgtc
     3001 cgccgctgtt gcccgtggga tcggtgcgga agtcgtcggg ggcagcgtga accacgagcg
     3061 cgctgccgtc cgcgtcgaac agcgacgtgg gccccgcccg cagcgtgaag cggtccgtcg
     3121 tggtctcgag acggccggtg ccgtcgggcg cgacggtcat gttcggaagg tcgccggcgt
     3181 gcgggccctc cgggttgagg atgccgtgct gccgcttgcc cgggttgaag tggccacccg
     3241 cggacgtgaa tcccgggggc tcgcaccggc ccacttcgtg gacgtgcacg gccctggcgc
     3301 cggccgggat gccgcggacc tccacgacga cgcgcacgcc accgctgacc tcggtgaacg
     3361 tcgccgtacc caccgtctgg ccgccggggc tcttgagctc ggcccgtgcc gtctccggcc
     3421 ggccgccgct cacggtcgca cacgcggcgg ccagccccga cagcgcgacc accgcgccca
     3481 gccgcttcat gccgagtggc cgccgcgcgc gaagtcgtgc ttggcgctct ggagcgcttg
     3541 cggcgcggcg agcaggtcga gcgcggtcag cgccatggcc ttggcgccgg ccagcatgcc
     3601 gacgcgcgcg atgggcgatc gcgcccactc ggcaaactcg cgcgagtggc cagggacccc
     3661 gtccggagag atgcggacat agggatggat cgtcgggatg gcctggctca cgttgccgca
     3721 gtccgtcgag ccgaaccccg cctctccgtc gtcggggtct tccggaaagt cgatcctctc
     3781 gaggttcttc cggaagagac cggccatcgt ggcgttgggc ctgagcggct cgtgcatcgg
     3841 accgtcctgc ccgatcctca cctggcatcc cgtggccacc gccgcgccct gggccaccga
     3901 ctcgaaccga cggagcagcc cgtgacagta ctcgcggtcc agcgcgcgga gatagaactc
     3961 ggcgctggtg tactccggga tgatgttggg ctgggcgccg ccgttcctga tgacgccgtg
     4021 gacccgggcg tccgggcgga gctgctggcg catcgcgtcc acggccacga agagctggat
     4081 gacggcgttg agcgcgttca ctccgcgcca cggccacgac gaggcgtgcg cggccttgcc
     4141 aaagtactcc accttcacct tgatgatccc caggctcggc cgccacacct gcgtgccgca
     4201 gcggccgtgg accatcatcg ccgcgtccac gtcgcggaag acaccggctt ccatcagctt
     4261 caccttgccg gccccgccct cctcggcggg agtgccgatg atctgcacgc agccggggaa
     4321 gggtagcgtc cccagcgccc gcgccagcgc ggcgccggcg cccgcgccgg cggtggcgat
     4381 gacgttgtgg ccgcaggcgt ggccgatgcc cggtagcgcg tcgtactcgc acaggatggc
     4441 aatcgtcggg cccggcgccg tgcccgggat cgtcgcgcgg aacgccgtgg gcaggccgcc
     4501 cacaccgcgc tcgacccgtg cgccgtgctt ctcgaggaag tccgtgagcc aggcctgcgc
     4561 cttctcctcg ccgaagccga gctcggggtg gtcgtggatc cggtgggaca gcgcctccag
     4621 ctcgttggcg agaccgtcgg cggccgcggc gatcccggct ttcagatcgt cggtcgtcat
     4681 cgcgcgcccg ccatctcctg cttcgcccgc gccaccaacg tagggtcgga gagaaggtcc
     4741 agggtggtca tggccatggc cttggccgcc gccagcaggc cctcgcgcgc cagggggctc
     4801 acggccgcag cctcgaacgc acgcgagtgg atcggcgtgc cgtcgggcgc gatcttcatc
     4861 aggggctgga tcgtggggac cacctggctc acgttgccca cgtccgagga gccgagccgg
     4921 tccttgacct cgggcacctc tgtcaggccc aggcgccgga gattctggcg ccacgcctcg
     4981 agcagggtct cgttgcgccg catgggctcg taggcgtcgt cgcgctcggt ggtcttcagc
     5041 gagctgccgg tggcccgggc cgcgccctcg gcgcacgcga tgacgcgctt ctgcagcgcc
     5101 cacatgtagt cgaggctggc cgaccgcacg tagaaggtcg ccgcggcgta ctccgggatg
     5161 atgttgggcg cggcgccgcc gctcgtgatg atgccgtgga tgcgcgcgtc cggccgtgtc
     5221 tgctggcgca gcatgctgac gttgttgaag gtctggatga cggcgtcgag cgcgttgacg
     5281 ccctcccagg gatccgcagc ggcgtgcgcg gccttgccgc tgaactcgaa gcccacgcgc
     5341 acgatgccga gcaggtcctg gtgcgcgatc cacctgtccc agccgtggca catcatcgcg
     5401 gcgtccacgc cctcgaacac gccgccgcgg atgagccgga tcttgccgcc gccgccctcc
     5461 tcggccgggg tgccgatcac gtggatgcgg cccttgggta gtcgatcgcg cagcgccgcg
     5521 agaccggcgc cggcgccggc gccggcggcc gcaatggcgt tgtggccgca cgcatggccg
     5581 atcgagggca gcgcatcgta ctcgcacatg atcgcgatgg tgggcccgtc gccggagtcg
     5641 agcgtggcgc ggaacgc
//
LOCUS       DATIAZ010000023        13824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1846163, whole genome shotgun sequence.
ACCESSION   DATIAZ010000023 DATIAZ010000000
VERSION     DATIAZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846163"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1404)
                     /locus_tag="VLF19_00750"
     CDS             complement(157..1404)
                     /locus_tag="VLF19_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91804.1"
                     /translation="MSAGPPREIAVYRPFALLAFAATVLVGTPLGVRMLAWLYLGASA
                     VTVDTVLLHAHLQTVGFFGTLIPGVAHHLVARFTGRPTRPTRATPWMAALLGTAGVLR
                     IACTWSAPAGLLVAALLETAAFTVFGAWILRSLAPPPLTTVRRHLAAATAWLGVACLA
                     EAALRGSALAGGVPTPDLGGMRAAHAMAIYGGAIGWVLGVLLRAGPMFVEGWRVPGLL
                     ATTLPGLLAAAVMVTAAGEIAAVPAIARAGEALALGAVVAVALAAGALRPAPRALPML
                     SRSAPEARIFRLGVVSAAFALCGAVGAAALAAAGAGVHVLTDAVRHLVTVGFLTSVVV
                     AMAFRLIPTLEATSLPWPGLRVVAFWSLLGATVLRTTQVAVAHGWPVLAPIVPVSGLL
                     AWVALAGVGATLLGTLHRVASRR"
     gene            1784..2425
                     /locus_tag="VLF19_00755"
     CDS             1784..2425
                     /locus_tag="VLF19_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSE91805.1"
                     /translation="MVAREHPELDAALARAGDGVFTVAPDGRIVLWNRAAERLLGFTA
                     REVLGKPCCDVFSGHDDKGNRLCYQGCHVRTLVKLGESVQNFDMRTRTKGGRPVWLNF
                     SILTLPEGAPGGGTTIHLFRDVTSTKELLTFVHEKLAAAASGGEPSPASALTRREVEV
                     LRLLAAGANTKAMADRLHVSPATIRNHVQNIGGKLGVHSRLEAVAYATRHRLL"
     gene            2536..4227
                     /locus_tag="VLF19_00760"
     CDS             2536..4227
                     /locus_tag="VLF19_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE91806.1"
                     /translation="MLRRWEDGKPRNYWDDDVHLRAALAWRLGRARLSAVEAALRQAG
                     ADSAGPVGALCRELDRPEHLPRLERWDAIGERTEDVAFHPDHHEIGRFVWRSGVLAVL
                     REPGRVTEHVALSYLWALNGEAAHLCSIACTAGLIKAVQRAGTDWMRREWLPWLLASD
                     YDRRWHGAQFLTEVQGGSDVGANTCVATPEPGRPGAWRVSGEKWFCSNVSADLYAVSA
                     RPQGAADGTRGLGLFVVPRRREDGGPNLAFARRLKQKLGTRTLPTAEMDFDGALAWQL
                     GSLEDGFRLMMGVVVNTSRLAVATGASGAMRRAFVEALHYARVREAFGHRLIEFPAVR
                     RQLADMRARAVAALTATLAMAAVEDRLTEAGVPPEADSLFRTGVILTKYLASLDAGRV
                     VHDAIEILGGNGTIEDFSPLPRLWREAPVQESWEGPHNTLMAQLLRDAERAKMHDALF
                     DRVEEALLAAGGPAGGAVVERALAARDELRERFVALLRGGGDRAALHVRPLAHRMGRL
                     FQVALLLEQAAAAAEPRPLVAVAEWLLHAEVISGHDPADDPEYAARILAILAPEQ"
     gene            4387..5013
                     /locus_tag="VLF19_00765"
     CDS             4387..5013
                     /locus_tag="VLF19_00765"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSE91807.1"
                     /translation="MAIATRYNDILNAAGEVFGRRGYHQASIREIARACGLSLAGLYH
                     YVGGKDELLFLTLDRALDTLRGALERARATARTPELRLLALIRTHLDFGFHHPSELKV
                     VNRDWEHLAEPRRSEIAAKRQAYVESGLAILRELDPHGRSGAELVSATNLLLGMLNGI
                     ARRPFLKSGDDTRVLAATVGALFLYGFLERSAVADGVLVTSGGGEHDD"
     gene            5003..6157
                     /locus_tag="VLF19_00770"
     CDS             5003..6157
                     /locus_tag="VLF19_00770"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HSE91808.1"
                     /translation="MTIDAILERCRERAGQPLGAVAHEWKAAHPGGRAIAVYPVWAPA
                     EIVHAAGMLPLALLGGGTSVELTHADARFQSFVCSIAKSSLELGFQGLVAGVDGFVFS
                     NICDVARNLGSIYKRNFPEAFVEYLHLPQNSTSPAVVGYLASELRRLAEGFTAAFDLG
                     VAETALAKSIDTYNALRARLRALYALRIAEPQKLSTVELYLVQRAAALAPPEEALGWL
                     DALLAGMPARSARPQDRMRVVVEGAFCEQPPLGLLEVLEEAGCYVVEDDLMLGWRWYT
                     GDVPGDGDPFERLAWAYVNRAVPSSTRHEAREHRAAGLIEKVRRARADAVVFTPAKFC
                     EPALFDYVLMKQGLEREGIPHLLVEFEEKMWTFERTRNEVETFVESMLFD"
     gene            6237..7505
                     /locus_tag="VLF19_00775"
     CDS             6237..7505
                     /locus_tag="VLF19_00775"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HSE91809.1"
                     /translation="MATPVTERQYQGRVHEVSRRLMADWMAQLGRAEAEGVPTGALMI
                     SGNCVELLRACHVLPMFPEVTALQNAIRHKSLPLILKAEQAGYSSDNCAYVKADIGLF
                     LEGGMGPGKPIPFPTITVCNYVGCNVYVKWFEHLADVTGSQLFMLDVPFARTGEASEA
                     DVRYVVAQLRELIALCERVSGRRFDIDRLREVLAHAARAEAGYARCKHLTKHHPAPFD
                     SYFDSINMMGPINVLRGTPEAADFFDVAAAEFESLVAQDLGPLSEERFRTVIEGPPPY
                     PYYKNFRNLFTKWGAVAVQSTYSTVGGIWEWGFRHDPRRPLESIAEQMLRENLTNRSI
                     TARYQQIRRYVEEWEADALVIHSIKSCRLFSAGQGDMRDYFARELGVPTLMVESDLED
                     PRYYAEAQIKNRIDAFFESLEHRKLTAAAH"
     gene            7519..10278
                     /locus_tag="VLF19_00780"
     CDS             7519..10278
                     /locus_tag="VLF19_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE91810.1"
                     /translation="MATTGVEKVPVYCYQCVAGPDLLKVVVRDGVAVGVEPNGDVAEH
                     PACGKVCVRAYGLVQKLYNPERVRTPLRRTNPRKGRNEPPRWAPISWEEALDLLASRL
                     NGLRARGLVDESGYPRLAVTFGSGGIAPAYLGTLAAFLAAWGPVDQGIGSGQGVKCYH
                     SEHLYGEFWHRAFTVAADVPRCDYVLSFGYNGDASGGVTGVWRHAEARARGIRWVQLE
                     PHQSVTAAGADEWVPIRPKTDAAALFALLHVILVEHDWRAVCDVPFLEGMTASPYLVG
                     PGGWYLRDPQTRKPLVWDLDRERPVPFDDARCLRPALTGAFIAAGVEVGADGATRTVS
                     ERVRPAFDRLIEHVRPYSPEWAAEICDVPAETLRRLAREYVAHARVGATVDVEGVTYP
                     HRPVAVLLGKTVTNGWGGYECCWARTVLAALVGALEVPGGILGTTVRLNRPAQNRLDS
                     VKAGPDGFMEQPLNATSRGQWQGAPHIRNAYRTLVPLADNSPWSAALGPAHLPWLFME
                     SPPEHWPAPTLPDVWIIYRTNPAISNWETDRIERELERFPFVAAFAYTQDETNWYADL
                     LLPEATDLESLQLYRVGGTKYIEQYWQHTGWAIRQPVTRPPFDTRDLTEVATELAART
                     GLLDAYLAALNRGAGTTVPLTGNGWDLAFSPGARPGADEIWDRVCRAATLSLTGGAVE
                     HDLEWFKRHGAFFVPFPARQYYLHGAMAARRLRYELPYQERIRRLGAELAARLHERGI
                     AWWDVQLEEYQALPAWKDFPAIWRDTAAAHGRRGEEFPLWLLTSRSMQYSWGSNVSLP
                     VLADVARNVRGHFGVMLNRRTAARLGIAEGDLVDIESPTGRTRGHAILREGVRPDVAV
                     ILQQFGHWATPFARDLEMPSLNQVAAMDLALTDATGSGADLVPVRVRKASAASVRAGK
                     RRTAR"
     gene            10349..10987
                     /locus_tag="VLF19_00785"
     CDS             10349..10987
                     /locus_tag="VLF19_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSE91811.1"
                     /translation="MTRWGMVIDVRRCVGCQTCTIACKQEHGLPAGTQWRFVADCEVG
                     EYPDVRRLFLPMQCMHCAEPPCVPVCPTGASRQREDGIVWVAYEACVGCGYCALACPY
                     HGRHLVHDGGTYFGEPTPAERATAQPARRGVMTKCTFCKERVDDGLARGLTPGADADA
                     TPMCAVACIARAIHFGDLDDPTSAVARMVVERRAVPLMPECGSEPSVYYVVE"
     gene            10989..11858
                     /locus_tag="VLF19_00790"
     CDS             10989..11858
                     /locus_tag="VLF19_00790"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSE91812.1"
                     /translation="MTTAAPVVLIPGRLQNLWRMPAVVNFAGGGLGAGLYVVAALASD
                     FLPAPALTVASWLGPLLVLGGFAAVATEAGRPLRGARVLARVATSWMSREVWIGAAFA
                     ALAACEFVIPHSLARLLALAAAVALVVAQGFIVRRARGLAAWNVPLMPFTFLVSSLVS
                     GAGGYLLVTLALGGPPGGRLLGATLAVLVLSALNWRAYLTWSADPAYVEATRPLREGA
                     TAREVVGIGHVAPFALVAAALVAPALAAPAAALAGALMIAVQVRAKAALILAAGQLRP
                     ITVAQLRLQRRPS"
     gene            11855..12739
                     /locus_tag="VLF19_00795"
     CDS             11855..12739
                     /locus_tag="VLF19_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HSE91813.1"
                     /translation="MIFGAGVDVGSTQTKAVIVDVSRTIVARSLIATGANVSRAGETA
                     FARACEAAGLSREAVSYVVGTGYGRYKVTFGDAQITEITCHARGAHSLFPATRTVIDM
                     GGQDTKAIKVGPDGSVVDFAMNDKCAAGTGRFLSAAADVTGLGLDEIGPRALQAKTPV
                     RLTSVCTVFVESDIMSYLAQRKTVEDILGGVHKAIATRTMALVRRVGVEDEVTFTGGV
                     SRNTGMVRALEAVLGRPVNVSEEGHFMGALGAALFALERAEAAAAHDAAPGARAEASA
                     PPDARAGLTPSAPMEART"
     gene            12736..13539
                     /locus_tag="VLF19_00800"
     CDS             12736..13539
                     /locus_tag="VLF19_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HSE91814.1"
                     /translation="MTLVAGIDIGSGTTKCCLVDQEGRPRGRGQVRTKADFEQVAREA
                     LDAALADAGATLPDVAYVATTGLGRYAIAARDIQITDLTCGARGAATLFPATRHVLDI
                     GAQCSRAIRLREGGKVKEFHMNEKCAAGSGGFLERAAKYLEVPVADIGGLSLNAARPQ
                     PISSVCAVLAESEIINHVSEGVSVENILRGIHNSLADRGLALLKRVGLDGEVTFIGGV
                     ARQEGMVSALREKLGVPVNVPTEPHLTTALGAALLGLQRFRRRAAAAAA"
     gene            13639..>13824
                     /locus_tag="VLF19_00805"
     CDS             13639..>13824
                     /locus_tag="VLF19_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE91815.1"
                     /translation="MSRTDVTIRPAEATDLDDIVRIDETLAGRTRKDYWQTRLDIAAL
                     RPPWMSLVAETDGRVVGF"
BASE COUNT         1836 a         5172 c         4976 g         1840 t
ORIGIN      
        1 ggggtccggc tccgtcgata gctcatgaat cgacgatacg ggacgcctcc gcggggcgtc
       61 aacgggcggc cccccgggcg taccatggcc accgcgggtg ccatcagccc acgcctggcc
      121 acgcaggcga gacgctggca cgcggcggcc cgcggcctat cgcctcgagg cgacccggtg
      181 gagcgtcccg agcagcgtcg cgccgacgcc ggcgagcgcg acccacgcga ggaggccgga
      241 gaccggcacg atcggcgcca gcacgggcca gccgtgggcg acggcgacct gggtcgtccg
      301 gagcaccgtg gcgcccagca gcgaccagaa cgccaccacc cgcagccctg gccacggcag
      361 cgacgtggcc tcgagcgtcg ggataaggcg gaatgccatg gccacgacga ccgacgtgag
      421 gaacccgacc gtgacgaggt ggcggacggc gtcggtcagg acgtgcacgc ccgcgccggc
      481 ggcggcgagc gccgccgcac cgacggcgcc gcacagcgcg aacgccgccg acacgactcc
      541 caggcggaag atgcgcgcct cgggggcgct gcgcgagagc atcggcagcg cgcgcggcgc
      601 cggccggagc gcgccggccg cgagagcgac ggccacgacg gcgcccagcg cgagcgcctc
      661 gccggcgcgc gcgatcgccg ggacggcagc gatctcgccc gccgcggtga ccatcacggc
      721 cgcggcgagg agcccgggaa gcgtcgtcgc gaggagcccc ggtacgcgcc agccctcgac
      781 gaacatcggc ccggcgcgca gcaggacacc gagcacccac ccgatcgcgc cgccgtagat
      841 cgccatcgcg tgggcggccc gcatgccgcc gaggtcaggc gtcggcacac cgccggcgag
      901 cgccgaccca cgcagcgcgg cctcggccag gcaggcgacg ccgagccacg ccgtcgccgc
      961 ggcgagatgt cgccgcacgg tcgtcagcgg tgggggggcc agcgagcgca atatccaggc
     1021 gccgaacacc gtgaacgccg ccgtctcgag cagcgcggcc accagcagac ccgccggggc
     1081 agaccaggtg cacgcgatcc tgaggacgcc ggcggtgccg agcagcgcgg ccatccaggg
     1141 cgtcgcgcgg gtcgggcggg tcgggcgccc cgtgaaccgc gcgacgagat ggtgcgcgac
     1201 gcctggaatc agcgtgccga agaagccgac ggtctgcagg tgagcgtgca gcagcaccgt
     1261 gtcgacggtc acggccgagg cgcccaggta gagccaggcc agcatgcgga cgcccagcgg
     1321 cgtgccgacc aggaccgtcg cggcgaaggc cagcagcgcg aacggccggt agacggcgat
     1381 ttcgcggggc ggccccgcgc tcatcgcgcc gctgtgcgac ccagggggcc ggccgcagcg
     1441 cgtcccacgt ggtgccgccg gcgcgggtcc gctggccggc ggccagcgtt ccgagcggct
     1501 atgccgtccc gtccccgcag agctctccgt cgcgtgtcca tcggcagctc atcgatcgac
     1561 gatagtggat cgtctcgcgg agcgtcaacg caccgctggg cgcgccggcc gaggggcctg
     1621 acggaaaccg gggcctcgct cgacgcgggc agaggcgggg cgcccgcgcg cggctgtggc
     1681 ggcggtgccc cgggtcggca gatggcggcg ctggacaaac atcgacctga cgcggggttg
     1741 gcgtccgtac gggtgtggta tcgcgcttgc ttcggaggtc ggtatggtcg cgcgtgagca
     1801 ccctgagctc gatgccgcgc tcgcacgagc gggcgacggc gtgttcaccg tggcgcccga
     1861 cggccggatc gtcctgtgga accgggcggc cgagcggctg ctcggcttca ccgcgcgcga
     1921 ggtcctcggc aagccttgct gcgacgtgtt ctccggacac gacgacaagg gcaaccgcct
     1981 ctgctaccag ggctgtcacg tccggacgct ggtcaagctc ggggagagcg tccagaactt
     2041 cgacatgcgc acgcgcacga agggcgggcg gcccgtgtgg ctgaacttca gcatcctcac
     2101 tctccccgag ggtgctcccg gtggcggcac gacgattcat ctctttcgcg acgtgacgtc
     2161 caccaaggag ctgctgacct tcgtccacga gaagctggcg gccgcggcga gcggcggcga
     2221 gccgtcgccc gccagcgcgc tcacgcggcg cgaggtcgag gtgctccggc tgctcgcggc
     2281 gggggcgaac accaaggcga tggccgaccg cctgcacgtg agcccggcga cgatccgcaa
     2341 ccacgtccag aacatcgggg gcaagctcgg cgtgcacagc cggctcgagg cggtcgcgta
     2401 cgccacccgc catcgcctgc tgtgagcgac gacgagcgcg gcccgacctc cgagcacggc
     2461 ctcgccccgc cggcgcccgc tgggccgtct gcgccgtccg gcgccacggc cccggacgcc
     2521 gcgcgcggtc ggtcgttgct gcgtcgatgg gaagatggca agcctcgcaa ctactgggac
     2581 gacgacgtcc acctgcgcgc cgcgctggcg tggcgcctgg gtcgcgcgcg gctctcggcc
     2641 gtggaggccg cgctccgtca ggccggcgcc gacagcgccg ggcccgtcgg cgcgctctgc
     2701 cgggagctcg atcggcccga gcacctgccg aggctcgagc ggtgggatgc gatcggcgag
     2761 cgtaccgagg acgtggcgtt ccatcccgat caccacgaga tcggccggtt cgtgtggcgc
     2821 tccggcgtgc tcgccgtgtt gcgtgagccg ggccgggtca ccgagcacgt cgccctgtcg
     2881 tacctctggg cgctgaacgg cgaggcggcg cacctctgct cgatcgcctg caccgcaggg
     2941 ctcatcaagg cggtccagcg cgcggggacg gactggatgc gacgcgagtg gctgccgtgg
     3001 ctgctcgcgt ccgactacga tcgtcgctgg cacggcgcgc agtttctcac cgaggtgcag
     3061 ggcggctccg acgtcggcgc caacacgtgc gtcgccacgc cggagcccgg ccggccgggc
     3121 gcgtggcgcg tcagcggcga gaagtggttc tgctcgaatg tctccgccga tctctacgcc
     3181 gtctcggcgc gcccccaggg cgccgccgac ggcacccgcg gcctcggcct gttcgtcgtg
     3241 ccgcgccgcc gcgaagacgg cggccccaac ctcgccttcg cgcgacgcct caagcagaag
     3301 ctcggtacgc gcacgctgcc cacggccgag atggatttcg acggcgcgct cgcctggcag
     3361 ctcggcagcc tcgaggacgg cttccgcctg atgatgggcg tggtcgtcaa cacgtcgcgc
     3421 ctggccgtcg ccaccggcgc ctcgggcgcc atgcgccggg cattcgtcga ggccctgcac
     3481 tacgcccgcg tacgcgaggc gttcggccac cggctcatcg agtttcccgc cgtgcgacgc
     3541 cagctcgccg acatgcgggc ccgcgccgtc gccgcgctca ccgccacgct ggcgatggcg
     3601 gcggtcgagg accggctgac ggaggccggg gtgccgcccg aggcggactc cctgttccgc
     3661 accggcgtga tcctcaccaa gtatctcgcc tcgctcgacg cgggacgggt ggtgcacgac
     3721 gccatcgaga tcctgggcgg caacgggacc atcgaggact tcagcccgtt gccgcgcctc
     3781 tggcgcgagg cgccggtgca ggagagctgg gagggcccgc acaacacact gatggcgcag
     3841 ctcctgcgcg acgcggagcg ggcgaagatg cacgacgcgc tgttcgaccg ggtggaggag
     3901 gctctgctcg ccgcgggcgg gccggccggg ggcgccgtgg tcgagcgggc gctggccgcc
     3961 cgcgacgagc tgcgcgagcg gttcgtcgcg ctgctgcgtg gcggcggcga ccgggccgcc
     4021 ctccacgtgc gcccgctcgc ccaccggatg gggcggctct tccaggtcgc cctgctcctc
     4081 gagcaggcgg ccgcggcggc cgagccccgg ccgctcgtcg ccgtcgccga gtggctgctg
     4141 cacgccgagg tcatctccgg ccacgacccc gccgacgacc ccgaatacgc cgcacgcatc
     4201 cttgcgatcc tcgcccccga gcagtagccg atctcttgag gccccggggg ccgattcgcg
     4261 ccggcgcagc tcgggtcgac cccgtctttc cgcactacaa aaatgaggtg atcggcggat
     4321 tccaagcctt taccgaacgg cctaccgtac gcccagcccg aacgatcgtt cggcgggagg
     4381 ttcggcgtgg cgatcgcgac acggtacaac gacatcctga acgccgccgg tgaggtgttc
     4441 ggtcgccgtg gctaccatca ggcctcgatc cgggagatcg cgcgcgcttg cggcctgtcg
     4501 ctcgcgggcc tctaccacta cgtgggcggc aaggacgagc tgctgttcct gacgctcgac
     4561 cgcgcgctcg acacgctgcg cggcgcgctg gagcgcgcgc gggccaccgc gcggacgccg
     4621 gagctgcggc tgctcgcgct gatccgcaca cacctcgact tcggcttcca ccatcccagc
     4681 gagctgaagg tcgtcaaccg cgactgggag cacctcgccg agccccgccg ctcggagatc
     4741 gccgccaagc gccaggccta cgtcgagtcg gggctcgcca tccttcgcga gctggacccg
     4801 cacgggcgaa gcggcgcgga gctggtctcc gcgaccaacc tgctgctggg catgctgaac
     4861 ggcatcgcgc ggcgcccctt cttgaagtcg ggggacgaca cccgcgtgct cgccgcgacc
     4921 gtcggcgcgc tgttcctgta cggcttcctg gaacgctcgg cggtcgcgga cggcgtgctc
     4981 gtgacctccg ggggaggcga gcatgacgat tgacgcgatc ctcgagcgct gccgcgagcg
     5041 ggccggtcag cccctcggcg ccgtcgccca cgagtggaag gccgcccacc cgggcggccg
     5101 ggccatcgcc gtctaccccg tgtgggcccc ggccgagatc gtccacgcgg ccgggatgct
     5161 gccgctcgcg ctgctcggcg gcggcacgtc ggtggagctg acacacgccg acgcccgctt
     5221 ccagtcgttc gtgtgctcca tcgcgaagtc ctccctggag ctcggcttcc aggggctcgt
     5281 cgcgggcgtg gacggcttcg tgttctccaa catctgcgac gtcgcgcgca acctcggctc
     5341 catctacaag cgcaacttcc cggaggcctt cgtggagtac ctgcacctgc cgcagaactc
     5401 cacgtcgccg gccgtcgtcg gctatctcgc cagcgagctg cgccggctgg ccgagggctt
     5461 cacggcggcc ttcgacctcg gcgtcgccga gaccgcgctg gccaagtcga tcgacaccta
     5521 caacgcgctc cgtgcccggc tccgggcgct gtacgccctg cgcatcgccg agccccagaa
     5581 gctctccacc gtggagctct acctcgtgca gcgcgcggcg gccctggcgc cgcccgagga
     5641 ggccctcggc tggctcgacg cgctgctcgc cggcatgccg gcccgcagcg cgcggccgca
     5701 ggaccgcatg cgcgtggtcg tggagggcgc gttctgcgag cagccgccgc tgggcctgct
     5761 cgaggtcctg gaggaggccg gctgctacgt ggtcgaggac gacctgatgc tcggctggcg
     5821 ctggtacacc ggcgacgtcc ccggcgacgg cgatccgttc gagcggctgg cctgggccta
     5881 cgtgaaccgc gcggtgccgt cctccacgcg gcacgaagcc cgcgagcacc gcgcggccgg
     5941 gctcatcgag aaggtgcggc gcgcgcgggc cgacgcggtc gtcttcacgc cggcgaagtt
     6001 ctgcgagccc gcgctgttcg actacgtcct catgaagcag gggctcgagc gcgagggcat
     6061 cccgcacctg ctcgtggagt tcgaggaaaa gatgtggacc ttcgagcgca cgcgcaacga
     6121 ggtcgagacc ttcgtcgagt cgatgctgtt cgactgagct gcacgcgatc gaggcccgtc
     6181 cgtcggagcc cggaggctcc cgacggggcc cccgacggca tatgaaagga cgtccgatgg
     6241 cgactcccgt gaccgagcgg cagtaccagg gtcgggtgca cgaggtctcc cgccggctca
     6301 tggccgactg gatggcgcag ctcgggcgcg ccgaggcgga gggcgtgccc accggggccc
     6361 tcatgatctc cggcaactgt gtggagctgc tccgcgcctg ccacgtgctg ccgatgttcc
     6421 ccgaggtcac cgcgctgcag aacgccatcc gccacaagtc gctgcccctc atcctcaagg
     6481 ccgagcaggc cggctactcc agcgacaact gcgcctacgt caaggcggac atcgggctct
     6541 tcctcgaggg cggcatgggc cccggcaagc cgatcccctt ccccaccatc accgtgtgca
     6601 actacgtcgg gtgcaacgtc tacgtgaagt ggttcgagca cctcgccgac gtgacggggt
     6661 cgcagctctt catgctcgac gtgcccttcg cgcgcaccgg cgaggcgagc gaggccgacg
     6721 tgcgctacgt ggtggcgcag ctccgcgagc tgatcgccct gtgcgagcgc gtgtccggcc
     6781 ggaggttcga catcgatcgc ctccgcgagg tgctggcgca cgcggcgcgc gccgaggcgg
     6841 gctacgcgcg gtgcaagcac ctcaccaagc accacccggc gcccttcgac tcgtacttcg
     6901 actccatcaa catgatgggc cccatcaacg tgctgcgcgg cacgccggag gcggcggact
     6961 tcttcgacgt ggcggcggcc gagttcgagt cgctcgtggc tcaggacctc ggccccctca
     7021 gcgaggagcg cttccggacg gtcatcgagg gcccgccacc ctacccgtac tacaagaact
     7081 tccggaacct cttcaccaag tggggcgccg tggccgtgca gtccacctac tccaccgtgg
     7141 gcggcatctg ggagtggggc ttccgccacg acccgcggcg gccgctcgag tcgatcgccg
     7201 agcagatgct ccgcgagaac ctcaccaacc ggtccatcac cgcccgttac cagcagatcc
     7261 gccgctacgt ggaggagtgg gaggccgacg cgctggtcat ccactccatc aagtcgtgcc
     7321 ggctcttctc ggccgggcag ggcgacatgc gcgactactt cgcgcgcgag ctgggcgtcc
     7381 ccaccctcat ggtggaatcc gacctcgagg acccgcgcta ctacgccgag gcccagatca
     7441 agaaccgcat cgacgcgttc ttcgaatcgc tggagcaccg caagctcacg gcggcggcgc
     7501 actgagacgg agccggccat ggccacgacg ggcgtcgaga aggttccggt ctactgctac
     7561 cagtgcgtgg ccggccccga cctcctgaag gtcgtcgtgc gcgacggcgt cgccgtcggc
     7621 gtcgagccga acggcgacgt ggccgagcac cccgcgtgcg gcaaggtctg cgtgcgcgcc
     7681 tacgggctcg tccagaagct ctacaacccc gagcgcgtcc gcacgccgct gcgtcgcacc
     7741 aatccccgga agggccggaa cgagcccccg cggtgggcgc cgatctcgtg ggaggaggcg
     7801 ctggacctgc tggcctcgag gctcaacggc ctccgcgcgc gcggcctcgt cgacgagtcc
     7861 ggctacccgc ggctcgcggt gacgttcggc tccggcggca tcgcgccggc gtacctcggc
     7921 accctcgccg cgttcctggc ggcgtggggg cccgtggacc agggcatcgg cagcggccag
     7981 ggcgtcaagt gctaccactc cgagcacctc tacggcgagt tctggcatcg cgcgttcacc
     8041 gtcgccgccg acgtgccccg ctgcgactac gtgctgtcgt tcggctacaa cggcgacgcc
     8101 tcgggcggcg tgaccggcgt gtggcgccac gcggaggccc gcgcgcgcgg catccggtgg
     8161 gtgcagctcg agcctcacca gtccgtcacc gccgccggcg ccgacgagtg ggtgccgatc
     8221 cggcccaaga ccgacgccgc cgcgctgttc gcgctgctgc acgtgatcct ggtcgagcac
     8281 gactggcgcg ccgtctgcga cgtgccgttc ctcgagggca tgacggcctc gccgtacctc
     8341 gtcggccccg gcggctggta cctgcgcgac ccgcagacgc ggaagcccct ggtgtgggac
     8401 ctcgaccgcg aacgccccgt gcccttcgac gacgcccgct gcctccgtcc cgccctcacc
     8461 ggcgccttca tcgccgcggg ggtcgaggtc ggcgccgacg gtgccacccg cacggtcagc
     8521 gagcgggtgc ggccggcctt cgaccggttg atcgagcacg tccggcccta cagcccggag
     8581 tgggcggcgg agatctgcga cgtgccggcg gagacgctgc ggcgtctggc ccgcgagtac
     8641 gtcgcccacg cccgcgtggg cgcgaccgtg gacgtcgagg gcgtgaccta cccgcatcgc
     8701 cccgtcgccg tgctcctcgg caagaccgtg accaacggct ggggcggcta cgagtgctgc
     8761 tgggcgcgca ccgtcctcgc ggcgctcgtg ggggccctgg aggtgcccgg cgggatcctc
     8821 ggcacgacgg tgcggctcaa ccgcccggcg cagaaccgcc tcgactcggt gaaggcgggc
     8881 cccgacgggt tcatggagca gccgctgaac gcgacgagcc gcgggcagtg gcagggcgcg
     8941 ccgcacatcc gcaacgccta tcgcacgctg gtgccgctcg ccgacaactc gccgtggtcg
     9001 gccgcgctgg gccccgcgca cctgccctgg ctcttcatgg agtccccgcc ggagcactgg
     9061 ccggcgccga cgctgcccga cgtgtggatc atctaccgca ccaacccggc catctccaac
     9121 tgggagacgg accgcatcga gcgcgagctc gagcgcttcc ccttcgtcgc cgccttcgcc
     9181 tacacccagg acgagacgaa ctggtacgcc gacctgctgc tgccggaggc gaccgatctc
     9241 gagtccctgc agctctaccg ggtggggggc accaagtaca tcgagcagta ctggcagcac
     9301 acgggttggg cgatccgcca gcccgtcacc cggccgccct tcgacacgcg cgacctcacg
     9361 gaggtggcga cggagctggc ggcccgcacc ggcctgctcg acgcctacct ggcggcgctc
     9421 aaccgcgggg cggggaccac cgtgcccctg acgggcaacg gctgggacct ggcgttctcg
     9481 cccggcgccc gcccgggggc cgacgagatc tgggaccggg tctgccgggc ggcgacgctg
     9541 tcgctcacgg gcggcgccgt cgagcacgac ctcgagtggt tcaagcggca cggcgctttc
     9601 ttcgtgccgt tccccgcccg ccagtactac ctgcacggcg cgatggcggc gcggcggctc
     9661 cgctacgagc tgccctacca ggagcgcatc cggcgcctgg gcgcggagct ggccgcgcgc
     9721 ctgcacgagc gcggcatcgc gtggtgggac gttcagctcg aggagtacca ggcgctcccc
     9781 gcgtggaagg acttcccggc catctggcgc gacaccgccg ccgcgcacgg gcgccgcggc
     9841 gaggagttcc cgctgtggct gctcaccagc cggagcatgc agtactcctg gggctccaac
     9901 gtctcgctgc ccgtcctcgc cgacgtcgcc cgcaacgtcc gcggccactt cggcgtgatg
     9961 ctcaaccgtc gcaccgcggc gcggctcggc atcgccgagg gcgacctggt ggacatcgag
    10021 tcgccgaccg ggcgcacccg gggccacgcc atcctacgcg agggcgtccg gcccgacgtc
    10081 gccgtgatcc tccagcagtt cggtcactgg gcgacgccgt tcgcgcgcga cctggagatg
    10141 ccgagcctga accaggtggc ggccatggac ctcgcgctca ccgacgccac cggctccggc
    10201 gccgaccttg tgcccgtgcg cgtccggaag gcgtccgcgg cgagcgtccg tgccgggaag
    10261 cggaggaccg cacgatgacg tgctggttgg cgaccctcgt tcggggcccc gcgcgggcgg
    10321 gcgacgccgt cgggcggcgg agcgcgcgat gactcgctgg ggcatggtca tcgacgtccg
    10381 ccgctgcgtg ggctgccaga cctgcaccat cgcctgcaag caggagcacg ggctgcccgc
    10441 gggaacccag tggcgcttcg tcgccgactg cgaggtcggc gagtaccccg acgtgcgccg
    10501 gctcttcctg ccgatgcagt gcatgcactg cgcggagccg ccgtgcgtgc cggtgtgccc
    10561 gaccggcgcc tccaggcagc gggaggacgg gatcgtctgg gtggcctacg aggcgtgcgt
    10621 gggctgcggc tactgcgcgc tggcctgccc gtaccacggg cggcacctcg tccacgacgg
    10681 cggcacgtac ttcggagagc ccacgcccgc cgagcgcgcg accgcgcagc ccgcgcgccg
    10741 cggcgtgatg accaagtgca ccttctgcaa ggagcgggtg gacgacgggc tcgcgcgcgg
    10801 cctcacgccc ggcgcggacg ccgacgccac gccgatgtgc gcggtggcct gcatcgctcg
    10861 tgcgatccac ttcggcgacc tggacgaccc gacgagcgcc gtcgcgcgca tggtcgtcga
    10921 gcgccgcgcc gtgcccctca tgcccgagtg cggcagcgag ccctcggtct actacgtggt
    10981 ggagtgagat gaccactgcc gcccccgtcg tgctgatccc cggcaggctg cagaacctct
    11041 ggcggatgcc cgccgtcgtc aactttgccg gcggcgggct cggcgccggc ctctacgtcg
    11101 tcgccgccct ggcctcggac ttcctgcccg cgccggcgct gacggtcgct tcatggctcg
    11161 gcccactgct cgtgctcggc ggcttcgcgg ccgtggcgac cgaggcgggc cggccgctgc
    11221 gcggcgcgcg cgtgctcgcc cgcgtggcga cgtcgtggat gtcgcgcgag gtgtggatcg
    11281 gcgcggcgtt cgcggcgctc gccgcctgcg agttcgtgat cccgcactcg ctggcgcgtc
    11341 ttctcgccct ggccgccgcc gtcgcgctgg tcgtcgccca ggggttcatc gtgcggcggg
    11401 cgcgcggtct cgccgcctgg aacgtgccgc tgatgccgtt caccttcctg gtctcctcgc
    11461 tcgtgtccgg cgccggcggc tatcttctcg tcaccctcgc cctgggcggg ccgccgggcg
    11521 gccggctgct gggcgcgacg ctcgccgtgc tggtgctgtc cgcgctcaac tggcgcgcgt
    11581 acctcacgtg gtcggccgat ccggcgtacg tcgaggccac ccggccgctc cgtgaaggcg
    11641 ccaccgcgcg cgaggtcgtg gggatcggcc acgtggcccc gttcgccctc gtggcggccg
    11701 cgctcgtggc ccccgctctc gccgcgccgg cagccgccct cgccggcgcg ctcatgatcg
    11761 cggtccaggt ccgcgccaag gcggcgctga tcctcgcggc gggacagctc cggcccatca
    11821 ccgtcgcaca gctgcgcctg cagaggaggc cctcatgatc ttcggcgccg gggtcgacgt
    11881 cggctccacc cagaccaagg ccgtcatcgt ggatgtgtcg cgaacgatcg tggcacggtc
    11941 gctgatcgcc accggcgcca acgtcagccg cgccggcgag accgccttcg cccgggcgtg
    12001 cgaggcggcc gggctgtcgc gcgaggccgt cagctacgtc gtcggcaccg gctacggtcg
    12061 ctacaaggtg accttcggcg acgcgcagat cacggagatc acctgccacg cccgcggcgc
    12121 tcactcgctg ttcccggcca cccgcacggt gatcgacatg ggcggccagg acaccaaggc
    12181 catcaaggtt ggcccggacg gctccgtcgt cgatttcgcg atgaacgaca agtgcgccgc
    12241 cggcaccgga cgtttcctct cggcggccgc cgacgtgacc ggcctcgggc tcgacgagat
    12301 cggcccacgc gcgctgcagg cgaagacgcc ggtgcggctc acgtccgtgt gcacggtctt
    12361 cgtggagtcc gacatcatgt cctacctcgc ccagcgcaag accgtcgagg acatcctcgg
    12421 cggcgtccac aaggccatcg ccacgcgcac gatggcgctc gtccgccgcg tcggcgtcga
    12481 ggacgaggtg accttcacgg gcggcgtgtc gcgcaacacc ggcatggtcc gcgcgctcga
    12541 ggcggtgctc ggccgcccgg tcaacgtgag cgaggagggc cacttcatgg gggcgctggg
    12601 cgccgccctg ttcgcgctcg agcgcgcgga ggccgccgcc gcgcacgacg cggcgcccgg
    12661 cgcgcgggcc gaggccagcg caccgcccga cgcccgcgcg gggctcacgc catccgcgcc
    12721 aatggaggca cgcacatgac cctcgtggcc ggcatcgaca tcggctcggg aacgacgaag
    12781 tgctgtctcg tcgaccagga gggcaggccc cgcggccgcg ggcaggtccg caccaaggcc
    12841 gatttcgagc aggtggcgcg cgaggcgctg gacgcggcgc tggcggacgc gggcgcgacg
    12901 ctgccggacg tcgcctacgt ggcgacgacg ggcctcgggc gctacgccat cgccgcgcgc
    12961 gacatccaga tcaccgacct gacctgcggc gcgcgggggg ccgccaccct gttccccgcc
    13021 acgcggcacg tgctcgacat cggcgcccag tgctcgcgcg ccatcaggct gcgcgagggc
    13081 ggcaaggtca aggagtttca catgaacgag aagtgcgcgg cgggctccgg cggcttcctg
    13141 gagcgcgcgg cgaagtatct cgaggtgccg gtggcggaca tcggcgggct gtcgctgaac
    13201 gccgcccggc cccagccgat ttccagcgtc tgcgcggtgc tcgccgagtc cgagatcatc
    13261 aaccacgtct ccgagggcgt cagcgtcgag aacatcctgc gcggcatcca caactcgctc
    13321 gcggaccgcg gcctggccct gctcaagcgc gtgggcctcg acggcgaggt cacgttcatc
    13381 ggaggcgtcg cccgccagga gggcatggtc agcgccctgc gcgagaagct cggcgtgccc
    13441 gtcaacgtgc ccaccgagcc gcacctcacc acggccctcg gcgccgcgct gctgggcctg
    13501 cagcgcttcc gcaggcgggc cgccgcggcc gccgcctgag cccgcggacg gaaggtgcag
    13561 tcgaggggcg ccacggcgcc cggagcaccg ggggccccgg gagagcccgg agcctccatg
    13621 gacagggagg atccgacgat gagccgcacc gacgtgacga tccggccggc cgaggccacc
    13681 gatctcgacg acatcgtgcg catcgacgag acgctcgccg gccggacccg caaggactac
    13741 tggcagaccc ggctggacat cgccgcgctc cgcccgccgt ggatgtcgct cgtggcggag
    13801 accgacggcc gtgtcgtcgg cttc
//
LOCUS       DATIAZ010000024         3960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_662617, whole genome shotgun sequence.
ACCESSION   DATIAZ010000024 DATIAZ010000000
VERSION     DATIAZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662617"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VLF19_00810"
     CDS             complement(<1..330)
                     /locus_tag="VLF19_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91816.1"
                     /translation="MSRRRARLARPLVTGGEGISFAPSPLRSARGAPPRGTRAWRYTG
                     IVGVAVFLSTSTALAAYEAVDVKDGGVLSGRVSFSGTPPKLEPIAVNKNRDVCGERKP
                     SEALLVGA"
     gene            complement(327..767)
                     /locus_tag="VLF19_00815"
     CDS             complement(327..767)
                     /locus_tag="VLF19_00815"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HSE91817.1"
                     /translation="MPFERQLGRAARIARRGAALRCPRCGETSLFLGGEPSRARAAAG
                     TSAVRRAVKRIEHWFTMRRECALCGLVFERAQGYFVGAIYINYAVTVTIAVGGYFLLW
                     SMTSISTATQLAIWVPFVLLFPLWFFRLSRSLWLALEYFVNPEP"
     gene            complement(777..1106)
                     /locus_tag="VLF19_00820"
     CDS             complement(777..1106)
                     /locus_tag="VLF19_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HSE91818.1"
                     /translation="MATTEHKHPNYMAIFWYLAVLTVVELAVIFMPLGKLTIGVLLCA
                     LAVTKAALVAMYFMHLRFEARTLGMVAVTPLAIATLLIFLLLPDGSAVDHKTAEKKGA
                     ATAPAKH"
     gene            complement(1119..1736)
                     /locus_tag="VLF19_00825"
     CDS             complement(1119..1736)
                     /locus_tag="VLF19_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSE91819.1"
                     /translation="MSQVAHVHGAVEPAETPLTPESWGKLGMWIFLAGDAVGFGVLLA
                     SYGVMRATSADWPNPYDVLGINLTAAMTFLLICSSVTMVKALEWLGRGDRARCKMFLL
                     FTALGGAIFVSLQAYEWTHLIGGGLHIDGNPWGAALFGTSFFIVTGFHGLHVSGGVIY
                     LLSMIRYVSNRPDPAASYNAVEITGLYWHFVDLVWIMVFTFMYLL"
     gene            complement(2140..2811)
                     /locus_tag="VLF19_00830"
     CDS             complement(2140..2811)
                     /locus_tag="VLF19_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSE91820.1"
                     /translation="MTPTLEAPPRRARANGHLVPPPPPPTGGGDGPDGDPPPRRVALD
                     NAVLGTLFFIGAEVMLFAGLVFSFWILRLAAPVWPPPLQPRLPVGVTGANTVVLLASS
                     VAMAAGLRTLSRGQRDAGVRRLATAAALGVVFVGVQGYEWLRLIGFGLTAASGAYGAT
                     FYTLIGAHGLHVLGAVTWLSITLWLAARGRFADGRSAPLRACAMYWHFVVALWPVLYV
                     SVYLL"
     gene            complement(2924..3817)
                     /gene="cyoE"
                     /locus_tag="VLF19_00835"
     CDS             complement(2924..3817)
                     /gene="cyoE"
                     /locus_tag="VLF19_00835"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSE91821.1"
                     /translation="MTEIVVDVIARDRRQVFADLVTLTKPRVVVMVVVTTLVGYYAAL
                     TGAPDYGRMLHLLLGTLLAAGGTLALNQYWERDVDSRMERTRSRPLPDGRLAPLEALA
                     FGAAVTAAGIVYLAACVNVLAAVVTAVITLLYLFAYTPLKLRTPLCTVVGAVPGALPP
                     VTGWVAAREDVAVGAWVLFGILFLWQLPHTLAIARLYREDYARAGVRVLPVVDVGGRS
                     TERQIVTGSLALLAVSLLPTLIGMAGPLYFAGAFVLGAGLASLGAWQALDPSLPAARR
                     VMFASLLYLPAVLALLALDKV"
BASE COUNT          682 a         1369 c         1454 g          455 t
ORIGIN      
        1 ggcgccgacc aggagggcct ccgacggctt gcgctcgccg cacacgtcac ggttcttgtt
       61 cacggcgatc ggctcgagct tcggcggcgt gccgctgaag ctcacccgcc cgctgaggac
      121 gccgccgtcc ttgacgtcca cggcttcgta cgcggccagc gccgtcgacg tcgagaggaa
      181 gaccgcgacc ccgacgatgc cggtgtagcg ccacgcgcgt gtcccgcgcg gtggcgcacc
      241 ccgcgcggac cggaggggac tcggggcgaa cgagatgccc tccccgccgg tcacgagcgg
      301 gcgtgccagc cgggcgcgcc ggcggctcac ggctctggat tcacgaagta ctccagcgcc
      361 agccacaggc tccggctcag ccggaagaac cacagcggga acaggaggac gaacggcacc
      421 cagatcgcga gctgggtcgc cgtcgagatc gacgtcatgg accacagcag gaagtacccg
      481 ccgacggcga tcgtcaccgt gaccgcgtag ttgatgtaga tcgcgccgac gaagtagccc
      541 tgcgcgcgct cgaagaccag cccacagagc gcgcattccc ggcgcatcgt gaaccagtgc
      601 tcgatccgct tcaccgctcg ccgcaccgcg ctcgtgcccg cggccgcccg ggcgcggctc
      661 ggctcgcccc ccaggaagag cgacgtctcg ccgcagcggg gacaccgcag cgccgccccg
      721 cgccgcgcga tccgcgcggc gcgccccagc tggcgctcga agggcacggc gcacgttcag
      781 tgcttggcgg gcgcggtggc ggcgcccttc ttctccgctg tcttgtggtc gacggcggag
      841 ccgtcgggca gcagcaggaa gatgagcagc gtcgcgatcg ccagcggggt gaccgccacc
      901 atgcccagcg tccgcgcttc gaaccgcagg tgcatgaagt acatggcgac cagggcggcc
      961 ttggtgacgg ccagggcgca cagcagcacg ccgatggtca gcttgccgag cggcatgaag
     1021 atgacggcga gctcgacgac cgtcagcacg gccaggtacc agaagatcgc catgtagttg
     1081 ggatgcttgt gctcggtggt cgccatcgct cccgccccct agagcaggta catgaaggtg
     1141 aagaccatga tccagaccag atcgacgaag tgccagtaga ggcccgtgat ctccacggcg
     1201 ttgtagctgg ccgccggatc gggccggttc gacacgtagc ggatcatcga cagcaggtag
     1261 atcaccccgc cggacacgtg caggccgtgg aatccggtga cgatgaagaa cgaggtcccg
     1321 aacagggcgg caccccaggg gttgccgtcg atgtgcaggc ccccaccgat caggtgcgtc
     1381 cactcgtacg cctgcaggct gacgaagatc gcgccgccga gcgccgtgaa gagcaggaac
     1441 atcttgcacc gggcgcggtc gccccggccc agccactcga gcgccttcac catggtcacg
     1501 ctcgagcaga tgagcaggaa ggtcatggcc gccgtcaggt tgatcccgag gacgtcgtag
     1561 gggttgggcc agtcggcgct ggtggcgcgc atgacgccgt aggacgccag cagcacgccg
     1621 aagccgacgg cgtcgccggc gaggaagatc cacatgccga gctttcccca gctctcgggc
     1681 gtgagcggag tctcggcggg ttccaccgcc ccgtggacat gggccacctg actcatgatc
     1741 tctcctcgga tggtggtgcg agccgagcgg cccccgagct gccggaggca tgagggccgc
     1801 gaggcgagtg gtgagagggg tcgtcggaat gtgcgagccg ggcggccccc gagctgtcgg
     1861 aggcatgagg gccgcgaggc gagctctgag atggctcgct cgagtgtgcg ggccccggca
     1921 cgagtgaccg gaagccgcgc ggcatggaca gcatgcggta ccggtagagc agcgtgccgc
     1981 cgatggcggc ggcgacgacg aacggcatgg cgatcagacc caggaacgcc cagttgaacg
     2041 tgcggtcccc gtatgcggac cacaggcacg tcgcgcaggc agcggcgacc gtgggccgcc
     2101 cgagcgtcgt cacgacgagg atcgcgagca cccggaccat cacagcaggt acaccgacac
     2161 gtagaggaca ggccacagcg cgaccacgaa gtgccagtac atcgcacagg cgcgcagcgg
     2221 cgccgatcgt ccgtcggcga accgtccccg cgccgcgagc cacagcgtga tcgagagcca
     2281 ggtgaccgca ccgagcacgt ggagcccgtg ggcgccgatg agggtgtaga acgtggcccc
     2341 gtaggcgccc gacgcggcgg tcagcccgaa gccgatcagc cggagccact cgtagccctg
     2401 gacgcccacg aagaccacac cgagcgccgc agcggtggcg agccgacgca ccccggcgtc
     2461 tcgctgcccg cgcgacagcg tgcgcaggcc ggcggccatc gccacgctcg aggccaggag
     2521 caccacggtg ttggcgccgg tcacgcccac cggaagccgc ggctgcagcg gcggcggcca
     2581 caccggggcg gccaggcgca ggatccagaa ggagaacacg agccccgcga agagcatgac
     2641 ctccgcgccg atgaagaaca gcgtccccag cacggcgttg tcgagggcca cacggcgcgg
     2701 cggcggatcg ccgtcggggc cgtcaccgcc gccggtgggg ggcggcggcg gcggcacgag
     2761 gtgaccgttc gcccgcgcgc gtcgtggcgg cgcctcgagg gtcggggtca ccagcggatc
     2821 cccacgagga gcgcggccag cgcgagcgcg cccatcacgg ccagcaggat cctcaggacc
     2881 tgtcggttgc ggcggtgcag ctcgtcgtcg cgcacgatcg cgctcatacc ttgtcgaggg
     2941 ccagcagggc caggacggcc ggcaggtaga gcagcgaagc gaacatgacc cgccgagccg
     3001 ccggcaacga cggatcgagc gcctgccagg caccgagcga cgcgagcccg gcgcccagca
     3061 cgaaagcgcc ggcgaagtag agcggcccgg ccatgccgat cagcgtgggc agcaggctca
     3121 ccgccagcag agcgaggctg ccggtcacga tctgacgctc ggtgctccgg ccaccgacgt
     3181 ccacgaccgg cagcacgcgc acgcccgcgc gcgcgtagtc ctcgcgatag agacgcgcga
     3241 tcgccagcgt gtgggggagt tgccagagaa acaggatgcc gaacagcacc caggcgccca
     3301 cggcgacgtc ctcgcgcgcc gcgacccagc cagtcaccgg ggggagcgcg ccgggaacgg
     3361 cgccgacgac ggtgcagagc ggcgtccgga gcttgagcgg cgtgtacgcg aacaggtaga
     3421 gcagcgtgat gacggcggtc accacggccg cgagcacgtt cacgcaggcg gccaggtaga
     3481 cgatgccggc cgccgtcacc gcggcgccga acgcgagcgc ctcgagcggc gcgagccggc
     3541 cgtcgggcag cggccgggag cgcgtgcgct ccatgcgcga gtcgacgtcg cgctcccagt
     3601 actggttgag cgccagggtg ccgcccgccg ccagcagcgt gccgagcagg aggtggagca
     3661 tccgcccata gtcgggcgcg ccggtgaggg cggcgtaata gccgacgaga gtggtgacga
     3721 ccaccatgac gacgaccctg ggcttggtca gcgtgaccag atccgcgaac acctggcggc
     3781 ggtcgcgagc gatgacgtcg acgacgattt ccgtcatagc gggtgccgcg aggccgcgag
     3841 aggcgtggaa gtgccaagac gcggcgcgtc cgaggcgtga ggtgagcccg cgtgcgtcgg
     3901 gctcaccatg ccggtggtgg ctgcgcgtgc ctcaccgatt tgttccgccg gagaggccgg
//
LOCUS       DATIAZ010000025         4991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1320560, whole genome shotgun sequence.
ACCESSION   DATIAZ010000025 DATIAZ010000000
VERSION     DATIAZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320560"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1016)
                     /locus_tag="VLF19_00840"
     CDS             complement(180..1016)
                     /locus_tag="VLF19_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HSE91822.1"
                     /translation="MATSRSTRSGRPPAPGWRQRLHEIIFEADTPAGRAFDVALLVAI
                     LLSVLAVLLESIPDVRRGYGTALRAAEWTFTVLFTAEYGLRLISVTRPARYALSFFGV
                     VDLLAILPTYLALAIPETQSLMVIRAIRLLRVFRILKLARFLQDAELLIRALSASRRK
                     ITLFLGSLVTIVLIMGTLMYLIEGEKHGFTSIPRSMYWAVVTMTTVGYGDIVPRTALG
                     RMLAAFVMILGYSIIAVPTGIVSVELAQVSRPVSTQACPACGRQGHDVDARHCKHCGA
                     ML"
     gene            complement(1025..1417)
                     /locus_tag="VLF19_00845"
     CDS             complement(1025..1417)
                     /locus_tag="VLF19_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSE91823.1"
                     /translation="MKVIYEVKDRIARITINRPEAMNAMDPEVYEELSKAWTDVRDNA
                     DVRVAIVTGAGEKAFTAGADLKGMTSRTWEKADFWLTQKNMILNVTKTGIKAGIAAVA
                     YKGDMVDEAMVKTAREMLAFARSIGVTS"
     gene            1668..2507
                     /locus_tag="VLF19_00850"
     CDS             1668..2507
                     /locus_tag="VLF19_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HSE91824.1"
                     /translation="MTVTIGLLHPGEMGGALGEALRIAGSRVLWTSEGRSASTRARAG
                     AAGLEDAGSLAAVVQASEVILSVCPPHAALDLARAVAARRFAGLYVDANAVSPATTRE
                     IGGIVEKGGATFVDGGIVGPPPRQRGTTRLYLSGASARRVAALCEVGALEAIVLEGGP
                     GAASAMKVAYASWTKGSQALLMTVRALAAADGVEAALLAEWERSQPGLVARSEAAAQG
                     TARKAWRWIAEMEEIAATFAAAGLPDGFHLASAEIYRRLQGYKDAKAPPSIAEVARML
                     PRR"
     gene            complement(2593..3429)
                     /locus_tag="VLF19_00855"
     CDS             complement(2593..3429)
                     /locus_tag="VLF19_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSE91825.1"
                     /translation="MPELPEVEAARRAMRRAAVGRRIVAVRCADDPIVFEAPPARVRR
                     ALLGRRVLDAHRHGKHLWLALDARPWPVFHFGMTGGFHQPTRPGARLKAYGSRHPAEV
                     WPPRFTRLCLVLDDGNELAFADARRLGRIRLRHDPRDEPPVSLLGFDALRELPAPRRF
                     RELLRERSAPLKAVLLDQTFAAGVGNWIADEVLYQARLDPRRRAHTLEDQEVDRLRAK
                     LRSVIETSVRVLNDSDRYPRRWLFHDRWGRDPGALTSRGEPIRYLTISGRTTAWVPSV
                     QR"
     gene            complement(3441..4799)
                     /locus_tag="VLF19_00860"
     CDS             complement(3441..4799)
                     /locus_tag="VLF19_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSE91826.1"
                     /translation="MSPRGTIEQEYAQARPKSRALHERAARVMPSGVAHDGRAFSPFP
                     FYVERADGARKWDVDGHVYLDGWSGHGALILGHNHPAIVAAITEQARRGLHYSACHEL
                     EVRWAELIRACVPGAEKVRFTMTGTETTALAIRVARAATGRTKVVKFQGHFHGVHDAV
                     VSGVKDPFEIPMSAGVPRETLAATLVARHNDLAHVRELLDGHEVAAVILEPAAARSMV
                     APTNPAFLGALRELTRERGVVLIFDEVVTGFRLAPGGAQEHFGVTADLICLAKAVAGG
                     LPGAALVGRADLLDAIAFTGDTARDRTGRVADQGTYSATPLVAAAGVAALEILRTGQV
                     QRELNRLGDRLRAGMGQVLKTRGVRGCVYGASSIFRIHLGADAHELGLDDWTMDVTRL
                     DRGMGPLAAPLHLAMLIHGVDYNRGSAIGWLNAAMTDADIDQMVEAFDRSLVRLREDG
                     RL"
BASE COUNT          654 a         1794 c         1847 g          696 t
ORIGIN      
        1 cgaactactg cgagagcatg accgccccgc cgccgccgtc gaagccgtgg tggcgctggc
       61 tgttcggacg ctgacgcgcg gttcgtctcg tcggagccgc aggccgggcg catgtagggg
      121 tgaccgcgtc caggcgccgg ctctgcccgg gggtcgaggc ccccctccga tggcggggct
      181 cacagcatcg cgccgcagtg cttgcagtgg cgggcgtcga cgtcgtggcc ctggcggccg
      241 cacgcggggc aggcctgggt ggagacgggc cggctcacct gggccagctc gacggacacg
      301 atgccggtcg gcaccgcgat gatgctgtag ccgaggatca tgacgaacgc cgccagcatc
      361 ctgccgagcg ccgttcgcgg gacgatatcg ccgtagccga ccgtcgtcat cgtcaccacg
      421 gcccagtaca tgctccgcgg gatgctcgtg aatccgtgct tctctccctc gatcaggtac
      481 atcagcgtgc ccatgatgag cacgatggtc acgaggctgc cgaggaagag ggtgatcttt
      541 cggcggctgg cgctcagcgc cctgatgagc agctcggcgt cctggaggaa ccgggcgagc
      601 ttgaggatgc ggaagacgcg cagcaggcgg atcgcgcgga tcaccatcaa cgactgggtc
      661 tccgggatcg cgagcgccag gtacgtcggg aggatggcga gcaggtccac cacgccgaag
      721 aagctgaggg cgtagcgcgc gggccgcgtg accgagatca ggcgcaggcc gtactcggcg
      781 gtgaacagca cggtgaaggt ccactccgcg gcccgcagcg ccgtgccgta cccccgccgg
      841 acgtcgggga tgctctccag cagcaccgcg aggacgctca ggaggatggc gacgagcagc
      901 gccacgtcga aggcgcggcc ggccggagtg tcggcctcga agatgatctc gtggaggcgc
      961 tgcctccacc ccggcgccgg tggccgcccg gaacgcgtgg agcggctcgt ggccatggct
     1021 cgcgtcagga ggtgacgccg atcgaccggg cgaaggcgag catctcgcgg gccgtcttca
     1081 ccatcgcctc gtccaccatg tcgcccttgt acgccaccgc ggcgatgccg gccttgatgc
     1141 cggtcttcgt gacgttgagg atcatgttct tctgggtcag ccagaaatcg gccttctccc
     1201 acgtccggga ggtcatccct ttcagatcgg cacccgccgt gaacgccttc tcgcctgcgc
     1261 ccgtcacgat ggccacccgg acgtcggcgt tgtcccgcac atccgtccag gccttcgaca
     1321 gctcctcgta gacctcgggg tccatcgcgt tcatggcctc cggccggttg atcgtgatgc
     1381 gggcgatcct gtccttcacc tcgtagatga ccttcatgaa gcgtcatcct ccccctaagc
     1441 gggtcgcgct gagcctagca cagcgcgccg ggtcgccggc gggcgcgcgg acgggcgcgc
     1501 ctcatcgcgc ttcggtgtat cgttgacacg cgggcgatct tcgtcgtggc cgccgcgctc
     1561 atggtggtca ccgtcttcac ggtcttgcag ttccgacgcc gggcgctgcc ggcggccgcg
     1621 cgggtggcca ccgggtcggc gacggccggc tcttgaggag gtacgagatg acggtcacga
     1681 tcgggctctt gcacccgggc gagatgggcg gcgcgctcgg cgaggcactt cgcatcgccg
     1741 gctcgcgcgt gctgtggacc tccgaggggc gcagcgcctc cacgcgggcg cgcgcgggcg
     1801 ccgcaggcct ggaggacgcc ggctcgctgg ccgccgtggt ccaggcgagc gaggtgatcc
     1861 tgtccgtgtg cccgccccac gccgcgctgg atctggcgcg cgctgtcgcc gcccggcgtt
     1921 tcgccggcct ctacgtggac gccaacgccg tgtcgcccgc cacgacgcgc gagatcggcg
     1981 gcatcgtcga gaagggcggc gccaccttcg tggacggcgg catcgtgggc ccgcccccgc
     2041 gccagcgggg gacgacgcgc ctctaccttt ccggggcatc ggcccgccgc gtggccgcgc
     2101 tctgcgaagt cggcgccctc gaggcgatcg tgctcgaggg cgggccgggc gcggcctccg
     2161 cgatgaaggt cgcctacgcc tcctggacca agggctcgca ggcgctgctc atgaccgtgc
     2221 gcgcgctggc cgccgccgac ggcgtggagg ccgcgctgct cgccgagtgg gagcgctccc
     2281 agcccggcct cgtggcccgc tccgaagccg ccgcccaggg caccgcgcgc aaggcgtggc
     2341 gctggatcgc ggagatggag gagatcgcgg cgaccttcgc cgccgccggg ctgcccgacg
     2401 gctttcacct ggcgtccgcc gagatctacc ggcgcctcca gggctacaag gacgcgaagg
     2461 cgccgccctc catcgccgag gtcgcgcgta tgctgcccag gcgctgacgg ccgcggtcac
     2521 cggcctcgcc gagccgatca atggatcgac ggcgtggcac gacggatgcc gccgcctccg
     2581 cccgcccgac catcagcgct ggaccgacgg cacccaggcg gtcgttcgcc ccgagatcgt
     2641 gaggtagcgg atcggctcgc cgcgcgaggt gagcgcaccg gggtcgcggc cccagcggtc
     2701 gtgaaagagc cagcggcggg gatagcggtc gctgtcgttc agcacgcgca ccgacgtctc
     2761 gatgaccgac cgcagcttcg cgcgcaggcg gtcgacttcc tggtcctcca gcgtgtgcgc
     2821 ccgtcgcctc gggtcgagcc gcgcctggta gagcacctcg tcggcgatcc agttgcccac
     2881 gcccgcggcg aaggtctgat cgaggagcac ggccttgagg ggcgccgacc gctcgcgcag
     2941 cagctcgcgg aaccgccgcg gtgccggcag ctcgcggagc gcatcgaagc ccagcaggct
     3001 caccggcggc tcgtcgcgcg ggtcgtggcg cagccggatg cggcccaggc ggcgcgcgtc
     3061 ggcgaacgcc agctcgttgc cgtcgtcgag caccaggcag agtcgcgtga accgcggcgg
     3121 ccacacctcc gccgggtggc gcgagccgta cgctttgagc cgcgcgcccg gtcgggtcgg
     3181 ctggtggaac ccgcccgtca tcccgaaatg gaagacgggc caggggcgag cgtcgagcgc
     3241 gagccagagg tgcttgccgt ggcgatgcgc gtcgagcact cgccggccga gcaacgcgcg
     3301 ccgcacccgc gccggcgggg cctcgaagac gatcgggtcg tcggcgcagc ggaccgcgac
     3361 gatgcggcgt ccgaccgcgg cgcgacgcat ggcacgacgc gccgcctcga cctcgggaag
     3421 ctcgggcatc cgcgcgcggc ttagagccgg ccgtcctcgc gcaggcggac gagcgagcgg
     3481 tcgaaggcct cgaccatctg gtcgatgtcg gcgtcggtca tggcggcgtt gagccagccg
     3541 atggccgagc cgcggttgta gtcgacgccg tggatcagca tggccaggtg cagcggagcg
     3601 gccagtggcc ccatgccgcg atccagccgc gtcacgtcca tcgtccagtc gtcgaggccc
     3661 agctcgtggg cgtcggcgcc gaggtgaatg cggaagatcg aggaggcgcc gtagacgcac
     3721 ccgcgcacgc cgcgcgtctt cagcacctgg cccatgcccg cgcgcaggcg gtcgcccagg
     3781 cggttcagct cgcgctgcac ctgaccggtc cggaggatct cgagcgccgc gacgccggcc
     3841 gcggccacga gcggcgtggc gctgtaggtg ccctggtcgg ccacgcgccc cgtccggtcc
     3901 cgcgcggtgt cgccggtgaa ggcgatggcg tcgagcaggt cggcgcggcc gacgagcgcg
     3961 gcgcccggca ggccgccggc caccgccttc gcgaggcaga tgagatcggc cgtcacgccg
     4021 aagtgctcct gcgcgccgcc gggtgccagc cgaaagcccg tcaccacctc gtcgaagatc
     4081 agcaccacgc cgcgctcgcg cgtcagctcg cgcagcgccc cgaggaacgc ggggttcgtg
     4141 ggcgccacca tcgagcgcgc cgccgccggc tcgaggatca ccgcggccac ctcgtgcccg
     4201 tcgagcagct cccgcacgtg ggcgaggtcg ttgtgccggg cgaccagcgt ggcggcgagg
     4261 gtctcgcggg gcacgccggc ggacatcggg atctcgaagg ggtccttcac gccgctgacg
     4321 accgcgtcgt gcaccccgtg gaagtggccc tgaaacttca cgaccttggt ccgtcccgtc
     4381 gccgcgcgcg ccacgcggat ggccagcgcg gtcgtctccg tcccggtcat cgtgaagcgc
     4441 accttctcgg cgcccggcac gcaggcccgg atcagctcgg cccagcggac ctccagctcg
     4501 tggcaggccg agtagtgcag cccgcggcgc gcctgctcgg tgatggccgc gacgatcgcg
     4561 ggatggttgt ggccgaggat cagggcgccg tggccgctcc agccgtcgag gtagacgtgg
     4621 ccgtccacgt cccacttgcg ggcgccgtcg gcgcgctcga cgtagaacgg gaacggggag
     4681 aaggcccggc cgtcgtgggc caccccgctg ggcatgacgc gcgccgcgcg ctcgtgcagc
     4741 gccctcgact tcgggcgggc ctgcgcgtat tcctgctcga tggttcctcg tgggctcatg
     4801 gccacccagg atagcggacc tccgccgcgg cgcgccaccc cgacgagtgg cgccaatcgg
     4861 acgtcgctgg tggactcgcc ggccggggtg gggtcggggg cgcgggtccg ccgacccctg
     4921 atgcgcttcg cgttgtcgct cgggtgccat ttccatcttc gggatggggc ggggttccct
     4981 gggcggggaa c
//
LOCUS       DATIAZ010000026        10164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_531718, whole genome shotgun sequence.
ACCESSION   DATIAZ010000026 DATIAZ010000000
VERSION     DATIAZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531718"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VLF19_00865"
     CDS             <1..149
                     /locus_tag="VLF19_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HSE91827.1"
                     /translation="FRIGHLGGLNELDLLGSLAGVEMMLREQGVAVRPGSGLGAAEEF
                     LIGA"
     gene            complement(159..530)
                     /locus_tag="VLF19_00870"
     CDS             complement(159..530)
                     /locus_tag="VLF19_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91828.1"
                     /translation="MRRGCTLEPMKPAGRWITVSVLVAGSVALLAGAVPAQHTTSQGT
                     DLEQQMRKRRFVVLPKPGPEAVTQDVDRATEALAARRGFDDVVRESRSPIGRRPNLDY
                     DVKSGIQSRSVQGLGLVPLRP"
     gene            complement(527..988)
                     /locus_tag="VLF19_00875"
     CDS             complement(527..988)
                     /locus_tag="VLF19_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSE91829.1"
                     /translation="MARHPTWLRALFADDDLGAVIAAIRRAEAGTSGEVRVHFERRLP
                     PSARGDALVRAREVFARLGMARTAARNGVLIYLALNDHRLAVVGDEAVHARVGDGYWN
                     RLRDGLVERLRAGRAREAVVAAVEDVGEVLSQHFPRRRDDVNELSDDVSAG"
     gene            complement(990..1697)
                     /locus_tag="VLF19_00880"
     CDS             complement(990..1697)
                     /locus_tag="VLF19_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSE91830.1"
                     /translation="MVPPPPTSRVNDYAGLLAPAQTERLERLLAERERATGTQMVIAV
                     FRSLEGESLEDFSIRLAERWRIGRKGLDDGVILLVFVAERRVRLDVGYGLEPRIPDAV
                     AGRIIGEAVAPRFREGRYAEGLEAAVAAVYGRLESGRAGGAGKARRGPDVATLATLAV
                     LAVIVGFVVMSQLGAARRRGYTATRGGWSAPRDVYRSGWGWGGGHRGGGGGFSGGGGG
                     FSGGGGSFGGGGASGSW"
     gene            complement(1739..2335)
                     /locus_tag="VLF19_00885"
     CDS             complement(1739..2335)
                     /locus_tag="VLF19_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSE91831.1"
                     /translation="MGRGLLIGLLVVAVLAVAIGGWVVSVNNQLVRLEQGVNATWAQV
                     QNVYQRRADLIPNLVETVKGFAAQERTVLEEVTRARASASGVQLTPQALNDPKALERF
                     QAVQNQLSGALSRLLVTVERYPELKSNQNFLALQTQLEGTENRIAVERRRYNEAVRDY
                     NTRLRLFPGSLIAGVMGFQPKAFFEAAPGAAEPPKVKF"
     gene            2369..3199
                     /locus_tag="VLF19_00890"
     CDS             2369..3199
                     /locus_tag="VLF19_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSE91832.1"
                     /translation="MPESLPDYHVRARNTSAGHENKIHDDEVARRYGFTGGLVPGVTV
                     YAYLTHPLVEALGAPWLARGTASVRFVKPVLDDEEVHVTGTVTARAADGLTARVTATT
                     ASSGECAVLEATLPAGLPTPVNGALYREAALPAERPPASRSMLERLEPLGTPQALYDA
                     TRAAEYLERVSDALPIYRGRGGWVHPAFLLDQSNRALDRNVRLGPWIHVASRVRHLGG
                     ARMGESLATRGRVRSLYERRGREFVELDLLIVSGVTARPVAHVLHTAIYALPEPGRPR
                     "
     gene            3302..4510
                     /locus_tag="VLF19_00895"
     CDS             3302..4510
                     /locus_tag="VLF19_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE91833.1"
                     /translation="MDLTLAAEDLAFRDATRRWFEDNTPAIEPKTLDERKAWHRKMYD
                     AGFIGLLWPREYGGRGATPMQQAIVQDEMARASAPPPINGLGIGFIGPTIIVHGTDAQ
                     KQRYLKKMLTAEEIWCQLYSEPNAGSDLASLKTRAEDAGDHFVVNGQKIWTSSGPIAD
                     WGILLARTDSKVAKHKGISCVLLNMRQPGVEVRPLKQITGSSLFSEVFFTNARAEKSD
                     LIGQLNQGWAIAQTTLGYERGANSLGRVTRYLTAFNQLVRSARELRRGGRALIEDPVV
                     RAKLGRVFADLEVQRYAALRVLSALQKGEPISAASSITKLSYTEFEKRYFEIALEILG
                     PWGQLMAAPDDIVEEVSTSSGEPGTFATAFLWSRAGTIYSGSSEIQKNVIGERVLGLP
                     KEIRADRVKG"
     gene            4515..5633
                     /locus_tag="VLF19_00900"
     CDS             4515..5633
                     /locus_tag="VLF19_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSE91834.1"
                     /translation="MDFAFSSDQQLLKNAARQFLDDHCKPATVRLLWDDPRGESEAMW
                     KEMAQLGWLGLALPEAYGGSDLGLVETAILLEEMGRASYPGPYLPTVLTARALLEAGT
                     APQKDRWLPAIAAGGARATVALLDAELSWDLDATTSRAERAGSGWQLSGVKHFVPWAH
                     VADVLLVPARTPEGLGAFLIERGAAGLKLSPVAGMDLATRWVKVEMDGVPAGAEAVLG
                     TPGQARPLLERLLRAGAVGAAAEMLGAARRCLDMSVEYAKVREQFGQPIGSFQAIRHK
                     CAEMLLEVENSHSAVYYAAWARDAGAADADLAASVAKAYVGDAARKVCGESIQVHGGI
                     GFTWEYDLHLYFKRAKALEAMYGDADFHRELIVRRVAA"
     gene            5639..6814
                     /locus_tag="VLF19_00905"
     CDS             5639..6814
                     /locus_tag="VLF19_00905"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE91835.1"
                     /translation="MAEPAPPLDGIRVVDLTSYIAGSYAAMMLADLGADVVKVEALEG
                     DNFRELPGFFGWNRGKRSIAANVKTPEGRAIVDRLVERGDVFMENMRPGVADRLGVGH
                     ERLRGINPQIIYCSVTGFGSTGPYVDRPAFDPLLQAMSGLMTIQGFLGPPQYVRVAVT
                     DYYTASLAAQAVLAALFVRERTGRGQRVETSLLHGAMALNSGAMIDYPGKPTLARDNP
                     TYRLYQAGDGEWLFLACGNQNFWVKLCKAMEIAELADDPRFGSWLLRLDNREELLPIL
                     EAKFRSKPRDEWLAILAANDIPAGRVQPLAEFLNDPAVGHHDMMREYDHDEAGRLRVM
                     GQPLVFEETPTRDPGPPPTLGEHTDEILGELGYDQSAIADLRARNVVTSRVRPGPPR"
     gene            6811..7593
                     /locus_tag="VLF19_00910"
     CDS             6811..7593
                     /locus_tag="VLF19_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSE91836.1"
                     /translation="MTYDTIRYELADGVVTVTLNRPDVHNAMNDAMRGELLRCFTDIE
                     TDDAVRVVVVTGAGERAFSAGADIREFPAPQVPTRFRDSRRRLDFRSVMERCPQPIIA
                     AIRGYAFGGGLELALACDIRIASEDARLGLTEVDLAIIPGGGGTQRLPRIVGRGKALE
                     MILTAARIDAREALRIGLVERVVAPQEVLPAAQALARTLADKAPVALRYAKEAVVKGL
                     ELPLADGLRLEQDLATLLRTTEDRIEGAKAFLEKRKPRFKGV"
     gene            7594..8589
                     /locus_tag="VLF19_00915"
     CDS             7594..8589
                     /locus_tag="VLF19_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSE91837.1"
                     /translation="MPFSYYARLSRAKQVIYRKSDAITDVRLPRPELLRPLVEDLAAA
                     LASEEREATQQATERLIRALGQELSLPPVRVEVLAARPHARWGELHGLYTAERGHAPK
                     IQLWMRTAKQRRVVAFRTYLRTLLHEMGHHVDYTLLRLADSFHTEGFYRRESSLFHQL
                     VPSGGSTTMPTMEEYAKQPLAERIARLERTADELAAAVGGQSDAVLSRRPDAKNWAAK
                     EVVCHLRDTEESFMGRFEQIMAMDEPKFLAVHPDRWAEERQYLRCDVPDAIAAFRRRR
                     QEAVAYIRRLAPGQLARAGVHITRGRLTADDVVSMMAWHDDNHLAQLRRALEGRA"
     gene            8935..9381
                     /locus_tag="VLF19_00920"
     CDS             8935..9381
                     /locus_tag="VLF19_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91838.1"
                     /translation="MTGIDQTPSPRRAFTDSVVLTGGHRPRAHEPRPPAGIEAVVEAL
                     EAGDAAAMLRAWHAACLDALGSQRWEPMIDVGDAAWRIGQATGFKIAFAVKARQAYHV
                     ALYRAHKQSSVEGVQRVGQAFQTLGDREAVQQCAAVAARLTERVDP"
     gene            complement(9454..9711)
                     /locus_tag="VLF19_00925"
     CDS             complement(9454..9711)
                     /locus_tag="VLF19_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91839.1"
                     /translation="MDTRAAFVAVLVVLAVSACAEPEREWLKVNQPYSAEEFRRDIAA
                     CTQGSALDEECMKGRGWISVSAKRSEKSQEPIRAPASPRYR"
     gene            9756..>10164
                     /locus_tag="VLF19_00930"
     CDS             9756..>10164
                     /locus_tag="VLF19_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91840.1"
                     /translation="MDSGWGRGVWAALVEAGDRLAAVLPALLVMLVLVVIGLVLGLVA
                     RVVLTRLGRAVKLDRHLERWGLASSFRRSGIRRTPADLLGVVAFWAIFVLFASLGVDA
                     LGVPGSRSATAFLFAFLPPLFAATLILVVGWAVA"
BASE COUNT         1453 a         3751 c         3517 g         1443 t
ORIGIN      
        1 tgttccggat cggccatttg ggcggcctca acgagctgga cctgctgggc tcgctggccg
       61 gcgtcgagat gatgctgcgc gagcagggtg tcgcggtgcg cccgggctcc ggtctcggcg
      121 ccgccgagga gttcctcatc ggcgcctgag agcggggttc agggccggag ggggacgagc
      181 cccagaccct ggacgcttcg cgactggata ccgctcttga cgtcgtagtc gaggttcggc
      241 cggcggccga tcgggctccg cgactcgcgc accacgtcgt cgaacccgcg ccgggccgcg
      301 agggcttcgg tcgcccgatc gacgtcctgc gtgaccgctt ccggacctgg cttcggcagc
      361 acgacgaagc gccgttttct catctgctgc tcgaggtccg tgccctggga ggtcgtgtgc
      421 tgagccggca cggccccggc gagcagcgcc accgacccgg cgacgaggac cgagaccgtg
      481 atccaccgcc ctgcgggctt catgggctcc agcgtacacc cgcgcctcat ccggcgctca
      541 catcgtcgct cagctcgttg acgtcgtccc ggcgccgcgg aaagtgctgg ctcaggacct
      601 cacccacgtc ctcgacggcg gccacgaccg cctcgcgcgc gcgcccggcg cgcagccgct
      661 cgacgagccc gtcgcgcagc cggttccagt agccgtcgcc gacgcgcgcg tggacggcct
      721 cgtcgcccac gaccgccagc cggtggtcgt tcagggccag gtagatcagc actccgttgc
      781 gcgcggccgt ccgggccatg ccgagccgcg cgaacacctc gcgggcgcgc accagcgcgt
      841 caccacgcgc ggagggcggc agccgccgtt cgaagtgcac gcgcacttct ccggaggtcc
      901 ccgcctcggc acgccggatg gcggcgatca ccgcgccgag gtcgtcgtcg gcgaagagcg
      961 cgcgcagcca cgtcggatgc ctcgccacgt caccagctcc cgctcgcgcc gccgccgccg
     1021 aagctgccgc cgcctccgga gaacccgccg cctccgcccg agaacccacc gcctccgccg
     1081 cggtgcccgc cgccccaccc ccagccgctg cgatacacgt cccggggcgc ggaccagcca
     1141 cccctggtgg cggtgtaacc acgacgccgg gccgcgccga gctggctcat gacgacgaac
     1201 ccgacgatga ccgcgagcac ggccagtgtg gccagcgtgg cgacgtccgg tccgcggcga
     1261 gccttcccgg ctccgccggc gcgcccggac tcgaggcggc cgtacaccgc ggcgaccgcc
     1321 gcctcgagcc cttccgcgta gcgcccctcg cggaacctcg gagcgaccgc ctcgccgatg
     1381 atccgccccg ccaccgcgtc ggggatgcgc ggctcgagcc cgtagcccac gtcgaggcgc
     1441 acgcggcgct cggccacgaa gaccaggagg atgacgccgt cgtccagtcc cttgcggccg
     1501 atgcgccagc gctcggcgag ccggatcgag aagtcctcga gactctctcc ctcgagcgaa
     1561 cggaagacgg cgatcaccat ctgggtgccc gtcgcgcgct cgcgctcggc gagcaggcgc
     1621 tcgagccgct cggtctgcgc cggggcgagc agccccgcgt agtcgttgac ccgggacgtc
     1681 ggcggtggcg ggaccacgag ggcgagggtg aggacgacgg ccggcagcag cagcaggact
     1741 aaaacttcac cttcgggggc tcggcggcac ccggcgccgc ctcgaagaac gccttgggct
     1801 ggaagcccat caccccggcg atcaagctcc ccggaaagag ccggaggcgc gtgttgtagt
     1861 cgcggaccgc ctcgttgtag cggcgccgct cgacggcaat gcggttctcg gtgccctcga
     1921 gctgggtctg cagcgcgagg aagttctggt tggacttcag ctccggatag cgctcgacgg
     1981 tcacgagcag ccggctgagc gcgccggaga gctggttctg cacggcctgg aagcgctcga
     2041 gcgccttcgg gtcgttgagc gcctgcggcg tgagctgcac gccgctggcg ctggcccgcg
     2101 cccgcgtgac ctcctcgagc accgtccgct cctgggcggc gaaccccttg acggtctcga
     2161 cgaggttggg aatgaggtcg gcgcggcgct gatagacgtt ctgcacctga gcccacgtgg
     2221 cgttgacgcc ctgctcgagc cgcacgagct ggttgttgac gctcacgacc cagccgccga
     2281 tggcgacggc gagcacggcg accaccagca ggccgatcag gagccctctg cccatgtggg
     2341 aaccattata cgtgctagac tccgcgccat gcccgagtcg ctgcccgact accacgtccg
     2401 ggcgcgcaac acttcggccg gccatgagaa caagatccac gacgacgagg tagcccgtcg
     2461 gtacggcttc acggggggcc tcgtgcccgg cgtcaccgtg tacgcctatc tcacccaccc
     2521 gctggtcgag gcgctcggcg caccgtggct cgcacgcggc accgcgagcg tccgcttcgt
     2581 caagcccgtg ctcgacgacg aggaggtcca cgtgaccggc accgtcaccg cgcgcgccgc
     2641 cgacggcctc acggcgcggg tgaccgcgac gacggcctcc tcgggtgagt gcgccgtgct
     2701 cgaggccacg ctgccggcgg gcctgccgac gccggtcaac ggcgccctct atcgcgaggc
     2761 cgcgctcccg gccgagcggc cgccggccag ccgctcgatg ctggagcgac tggagccgct
     2821 cggcacgccg caggcgctct acgacgccac gcgcgccgcc gaatacctcg agcgcgtgtc
     2881 cgacgcgctg ccgatctacc gtggccgcgg gggctgggtc catccggcgt tcctgctcga
     2941 ccagtccaat cgcgccctcg accgcaacgt ccggctcggc ccgtggatcc acgtggccag
     3001 ccgcgtgcgc cacctcggtg gcgcgcgcat gggcgagtcg ctcgccacgc ggggccgtgt
     3061 ccgctcgctc tacgagagga gaggtcgcga gttcgtcgag ctggacctgc tgatcgtgag
     3121 cggtgtcacc gcccgacccg tcgcccacgt gctccacacc gcgatctacg cactgcccga
     3181 gcccggtcgg ccacgctgaa cctctcccgc tcggtgagcc acaccggccg gccctccggg
     3241 agcggcgttg cttcccccta cccgccgtgc tagaaacgtc catccagccg aggaggagcc
     3301 gatggatctg acactcgcgg ccgaggatct cgccttccgc gacgccaccc ggcgctggtt
     3361 cgaggacaac acaccggcga tcgagccgaa gaccctcgac gagcgcaagg cctggcaccg
     3421 gaagatgtac gacgccggct tcatcgggct gctctggccc agggagtacg gcggccgggg
     3481 cgcgacgccg atgcagcagg cgatcgtgca ggacgagatg gcgcgggcga gcgcgccgcc
     3541 gcccatcaac ggtctcggca tcgggttcat cggcccgacg atcatcgtcc acggcaccga
     3601 cgcgcagaag cagcgctatc tgaagaagat gctcacggcc gaggagatct ggtgccagct
     3661 ctactcggag ccgaacgccg gctccgacct cgcgagcctc aagacgcgcg ccgaggacgc
     3721 cggcgatcat ttcgtcgtca atggccagaa gatctggacg tcgagcggcc ccatcgccga
     3781 ctggggcatc ctcctcgcac ggaccgactc gaaggtggcc aagcacaagg gcatctcctg
     3841 tgtcctcctc aacatgcgcc agcccggcgt cgaggtgcgg ccgctcaagc agatcacggg
     3901 cagctcgctc ttctccgagg tgttcttcac gaacgcgcgc gccgagaagt ccgacctgat
     3961 cggccagctc aaccagggct gggcgatcgc ccagacgacg ctcggctacg agcgcggcgc
     4021 caactcgctg ggccgcgtga cccgctatct caccgcgttc aaccagctcg tgaggtcggc
     4081 cagggagctg cgccggggcg ggcgggccct catcgaggac ccggtggtgc gcgccaagct
     4141 cggccgcgtc ttcgccgacc tggaggtgca gcgctacgcc gcgctgcgcg tgctgtcggc
     4201 gctgcagaag ggcgagccca tcagcgcggc ctcctcgatc accaagctct cgtacacgga
     4261 gttcgagaag cggtatttcg agatcgcgct cgagatcctg gggccctggg gccagctcat
     4321 ggccgctccc gacgacatcg tggaggaggt ctccacgtcg tcgggcgagc cgggcacgtt
     4381 cgccaccgcg ttcctgtggt cccgggcagg cacgatctac tcgggctcgt cggagatcca
     4441 gaagaacgtc atcggcgagc gcgtgctcgg gctgcccaag gagatccgcg ccgaccgggt
     4501 gaagggatag cccgatggac ttcgcgttca gctccgacca gcagctcctg aagaacgccg
     4561 cccggcagtt cctggacgac cactgcaagc ccgccacggt gcggctgctc tgggacgacc
     4621 cccgcggcga gagcgaggcg atgtggaagg agatggccca gctcggctgg ctcggcctgg
     4681 ccctgcccga ggcgtacggc ggcagcgatc tcggcctcgt cgagacggcc atcctcctcg
     4741 aggagatggg ccgggcgagc tatccgggac cgtatcttcc gaccgtgctg accgcccgcg
     4801 ccctgctcga ggcgggcacg gcgccgcaga aggaccgctg gctgccggcg atcgcagccg
     4861 gcggcgcccg ggcgacggtg gccctcctcg acgccgagct gtcgtgggac ctcgacgcca
     4921 ccaccagccg cgcggagcgt gcaggcagcg gctggcagct ctccggcgtc aagcacttcg
     4981 tgccgtgggc ccacgtggcc gacgtgctgc tggtgcccgc gcgcacgccc gagggcctgg
     5041 gcgccttcct gatcgagcgg ggcgcggcgg ggctcaagct ctcgccggtg gcgggcatgg
     5101 acctcgccac gcggtgggtc aaggtcgaga tggacggcgt gcccgccggg gccgaggccg
     5161 tcctcgggac gcccggtcag gcccggcccc tgctggagcg cctgctccgc gcgggcgcgg
     5221 tgggcgcagc ggccgagatg ctcggcgccg cgcggcggtg tctggacatg agcgtggagt
     5281 acgccaaggt gcgggagcag ttcggccagc ccatcggctc gttccaggcc attcgtcaca
     5341 agtgcgccga gatgctgctc gaggtggaga actcgcactc ggccgtgtac tacgcggcgt
     5401 gggcgcgcga cgccggggcc gcggacgccg acctcgcggc ctcggtggcc aaggcctacg
     5461 tgggggacgc cgcgcggaag gtctgcggcg agtccatcca ggtgcacggc ggcatcggct
     5521 tcacctggga gtacgacctg cacctctact tcaagcgcgc caaggcgctg gaggcgatgt
     5581 acggcgacgc ggacttccac cgcgagctga tcgtccgccg ggtcgcggcc tgaccgccgt
     5641 ggctgagccc gctccgcccc tcgacggcat ccgcgtcgtc gacctcacga gctacatcgc
     5701 cggctcctac gcggccatga tgctcgccga cctcggcgcc gacgtggtca aggtggaggc
     5761 gctcgagggc gacaacttcc gggagctccc gggcttcttc ggctggaacc gcggcaagcg
     5821 ctcgatcgcg gcgaatgtca agacgccgga gggccgcgcc atcgtcgacc gcctggtgga
     5881 gcgcggcgac gtcttcatgg agaacatgcg gcccggcgtg gccgaccgcc tgggcgtggg
     5941 ccacgagcgg ctgcgcggga tcaatccgca gatcatctac tgctcggtca cgggcttcgg
     6001 gtccaccggc ccctacgtcg accgtcccgc gttcgacccg ctgctgcagg cgatgagcgg
     6061 gctcatgacc atccagggct tcctcggacc gccccagtac gtccgcgtcg cggtcaccga
     6121 ctactacacg gccagcctcg ccgcccaggc cgtgctcgcc gcgctcttcg tgcgcgagcg
     6181 caccgggcgc ggccagcgcg tggagacgtc gctgctgcac ggcgccatgg cgctgaactc
     6241 gggcgcgatg atcgactacc cgggcaagcc caccctggcc cgcgacaacc cgacgtaccg
     6301 cctctaccag gccggcgacg gcgagtggct gttcctggcc tgcggcaacc agaatttctg
     6361 ggtcaagctc tgcaaggcca tggagatcgc ggagctcgcc gacgatccgc gcttcggctc
     6421 ctggctcctc cgtctcgaca accgcgagga gctgctgcca atcctcgagg ccaagtttcg
     6481 cagcaagccg cgcgacgagt ggctggccat cctggccgcc aacgacatcc ccgccggacg
     6541 cgtccagccg ctcgccgagt tcctgaacga cccggccgtg ggccaccacg acatgatgcg
     6601 cgagtacgac cacgacgagg ccgggcgcct gcgcgtcatg ggccagccgc tggtcttcga
     6661 ggagacgccc acgcgggacc cggggccgcc gccgaccctc ggggagcaca ccgacgagat
     6721 cctcggcgag ctgggctacg accagtcggc gatcgccgat ctccgcgcgc gcaacgtcgt
     6781 gacctcgcgc gtgcgtccgg ggccgccgcg atgacctacg acacgatccg ctacgagctg
     6841 gccgacggcg tcgtcacggt gacgctgaac cgccccgacg tccacaacgc catgaacgac
     6901 gcgatgcggg gcgagttgct ccggtgcttc accgacatcg agaccgacga cgcggtccgc
     6961 gttgtcgtcg tcaccggcgc cggcgagcgc gcgttctccg ccggcgccga catccgcgag
     7021 ttcccggcac cccaggtccc cacgcgcttc cgcgacagtc gccggcggct cgacttccgc
     7081 tcggtcatgg agcgctgtcc ccagccgatc atcgctgcca tccgaggcta cgccttcggt
     7141 ggcggcctcg agctggcgct cgcctgcgac atccgcatcg cctccgagga cgcccgcctc
     7201 gggctcacgg aggtggacct cgccatcatc cctggtggcg gcggcaccca gcgcctgccg
     7261 cggatcgtcg ggcggggcaa ggcgctcgag atgatcctca cggccgcccg catcgacgcg
     7321 cgcgaggcgc tccgcatcgg cctcgtcgag cgcgtcgtgg cgccccagga ggtattgccg
     7381 gccgcccagg ccctggcgcg gacgctggcc gacaaggcgc cggtcgcgct gcgctacgcc
     7441 aaggaggccg tcgtgaaggg gctcgagctg ccgctcgcgg acgggctccg tctcgagcag
     7501 gatctcgcca cgctcttgcg caccaccgag gaccgcatcg aaggcgccaa ggccttcctc
     7561 gagaagcgca agccgcgctt caagggcgtc taggtgccgt tctcctacta cgcgcgcctg
     7621 agccgcgcga agcaggtgat ctaccgcaag agcgacgcga tcacggacgt gcggctgccg
     7681 cgccccgagc tcctgcgtcc gctggtggag gacctggcgg cggcgctcgc ctccgaggag
     7741 cgcgaggcca cccagcaggc cacggagcgg ctcattcgcg ccctcggcca ggagctgagc
     7801 ctgcccccgg tgcgggtgga ggtgctggcg gcgcgcccgc acgcccgctg gggcgagctg
     7861 cacggcctct acaccgccga gcgcgggcac gcgccgaaga tccagctctg gatgcgcacg
     7921 gcgaagcagc gccgcgtcgt ggcgttccgc acgtatctga ggacgctgct ccacgagatg
     7981 ggccaccacg tggactacac gctgctccgt ctggccgact cgtttcacac cgagggcttc
     8041 tacaggcggg aatcgagcct cttccaccag ctcgtcccgt caggagggtc cacgacgatg
     8101 ccgacgatgg aggaatatgc caagcagccg ctggccgagc gcatcgcacg cctcgagcgc
     8161 accgccgacg agctggccgc cgcggtcggc ggccagagcg acgccgtgct gtcccgacgg
     8221 cccgacgcga agaactgggc cgcgaaggag gtcgtctgcc acctgcgcga caccgaggag
     8281 tccttcatgg ggcgcttcga gcagatcatg gccatggacg agccgaagtt cctcgccgtc
     8341 catcccgacc ggtgggccga ggagcgccag tacctccgct gcgacgtgcc cgacgcgatc
     8401 gcggcgttcc ggcgccgacg ccaggaggcc gtcgcgtaca tccgcaggct cgcgcccggc
     8461 cagctcgcgc gggccggcgt gcacatcacc cgaggccggc tcaccgccga cgatgtcgtc
     8521 tcgatgatgg cctggcacga cgacaaccac ctggcccagc tcaggcgcgc gctcgagggt
     8581 cgggcctaga ccagcgaggg ggcacaggta gcccagaagc aggccgatcg ccgagtcagg
     8641 tcgccgccgc ctgaccacct cctgaccgtt caatttttca ccactaccac cgtgccgagc
     8701 ggttctacca gccgcaagtg tctgtttacc ttgagatcgt ggagtggcat ctcttgtgcg
     8761 tcccagcggt tccgaggcac acgagctgag tcgcggccgg aggcgcagca tgagagagcc
     8821 atcgacgttc accgatcgcg cgtgcgtggc gatcgttgca ctcctggtgc tcgcgttggt
     8881 cgtgggattc tccatcctgg aggcgccgag cagcgccggc gggtcggtac tgcgatgacc
     8941 ggcatcgatc agaccccctc gccccggcgc gccttcaccg actcggtcgt gctgaccggt
     9001 ggccaccggc cgcgggccca cgagccacga ccgccggctg gaatcgaagc cgtcgtggag
     9061 gcactcgagg cgggcgacgc cgccgcgatg ctgcgtgcct ggcacgctgc gtgcctcgac
     9121 gcgctcggca gccagcgatg ggagccgatg atcgacgtcg gcgacgctgc ctggcgcatc
     9181 gggcaggcga ccggcttcaa gatcgcgttc gccgtaaagg cccgccaggc ctatcacgtc
     9241 gcgctgtaca gggcccacaa gcagagcagc gtcgagggcg tgcaacgggt cggccaggca
     9301 ttccagacgc tcggcgaccg cgaagccgtc cagcagtgcg cggccgtcgc cgcaaggctg
     9361 accgagcgag tcgacccttg agccatgtcg atgaggctgg cggccttcgg catgtcccgg
     9421 cgcagccggg acgcgccctc tcgaaccgac aggctatctg taacgcggac tggccggcgc
     9481 ccggatgggc tcctgagact tctcggacct cttcgccgac acgctgatcc acccgcgccc
     9541 cttcatgcac tcctcgtcca gcgcgcttcc ctgcgtgcag gcggcgatgt cacgccggaa
     9601 ctcctcggca gaatagggct ggttgacctt cagccactcc cgctccggct cggcgcaggc
     9661 ggacaccgcg agcaccacga gcacggcgac gaatgcggcc cgggtatcca ttggccccgc
     9721 attgtaccgg gtgtatcgtc tatgcggtaa ctcccatgga ctccggctgg ggacggggtg
     9781 tctgggcggc gctggttgag gcgggtgatc gcctggcggc ggtcctgccc gccctgctgg
     9841 tgatgctcgt tctggtcgtg atcgggctcg tgctcggact cgtcgcgcgt gtggtgctca
     9901 cgcggctcgg ccgggccgtc aagctcgacc ggcacctgga gcgctggggg ctcgcctcct
     9961 cgttccgccg ctcgggtatc cggcggacac cggccgacct cctcggcgtg gtggccttct
    10021 gggcgatctt cgtgctcttc gcgagtctgg gcgtcgatgc gctcggcgta ccgggctccc
    10081 gcagcgcgac ggcgttcctc ttcgccttcc tgccgccgct cttcgcggcg accctgatcc
    10141 tcgtcgtcgg ctgggccgtc gcca
//
LOCUS       DATIAZ010000027         8820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_663316, whole genome shotgun sequence.
ACCESSION   DATIAZ010000027 DATIAZ010000000
VERSION     DATIAZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8820
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663316"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..959
                     /locus_tag="VLF19_00935"
     CDS             3..959
                     /locus_tag="VLF19_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HSE91841.1"
                     /translation="MTLDDVKAAARRLQGHVTRTPVVTSQSWDDATGYRVFFKCENLQ
                     RAGAFKIRGALNKLLTLTPEERARGVIAFSSGNHAQGVALAARMLGTTAVIVMPRDAP
                     ALKLEATRGYGAEVVFYDRQADDREAIARRLAEETGRMLVPPYDDYSIMAGQGTAALE
                     LFEEVPSLDALVAPVGGGGLMAGCSTVGRALAPGMTIVGVEAETANDTWLSLEKGERV
                     TIPPPPTIADGIRITSPGALTFPVLVRNRVEVVLVSDDEIRTAVRLLALRAHLVTEPT
                     GAVGAAAALARRIGVRAGGRVGVVLSGGNVDPEMLIDILRAR"
     gene            complement(1050..2663)
                     /locus_tag="VLF19_00940"
     CDS             complement(1050..2663)
                     /locus_tag="VLF19_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HSE91842.1"
                     /translation="MATLHQLQTVVLLLIAVLVLVTLAQRVLIPYPILLVLGGLALSL
                     VPGVPVVPLQPDLVFFIFLPPILWAAAYFTSLRDFRANLRPITLLAVGLVLATTAAVA
                     AVARLILPGLSWPAAFALGAIVSPPDAVATTAIARRLHIPHRLVTILEGESLINDATA
                     LVLYRVAVAAVVTGSFVLGETPWLLVATALGGTAIGLVVGAVARRALRLTADSLAETA
                     VTLLAPYAAWVLAERMHTSAVLACVVGGLYLRQGFSEVVAPATRLQARAVWDLLVFVL
                     NGVIFILIGLQLAAIREAAPPGTLAPLLVKGAVISATAIAVRLVWVPLAAVIPRLLSP
                     SLRRRDPMPPWQALFLLGWAGMRGIVSLAAALALPLTTASGAPFPFRDEITVLSFVVI
                     LSTLVVQGLSLPRLIRVLNLGEDATLELEEVQAREAAARAALARLDEVTGAPWARAAQ
                     VDRLRAIYTQRLRRSSPLTAGQDRMAAEGQASYRRLRHETLSAERRAVIALRDQGAIS
                     DEVLHRIEQELDVEATRIGLGEARLETEA"
     gene            complement(2718..3650)
                     /locus_tag="VLF19_00945"
     CDS             complement(2718..3650)
                     /locus_tag="VLF19_00945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSE91843.1"
                     /translation="MKIKYRIGVMPGPWPAGGGVEHLWRLLDLCERTDIDSVWFNERL
                     SSPLPVLEPVTAMAAVAARTRRLKFGPSVLITPFRTPVLLARELAMLDWLSGGRMLPA
                     LGVGVEQEREFRAAGVPFKERGRRTDEAISIMRHCWAEDEVTFAGEFWQLEKVSVLPK
                     PIQRPFPVWIGGNSEAAMRRAGRLGDGWIPSFIRPEQVRVGIEKTQAFAAEAGREVPT
                     DHFGTLVYFGFDDDAARARATAQPFIPRGRTDEATLAAVTAFGPPAVLAERLEQYVRS
                     GASKFIVRPMVPPDQVLDQLAKLAEEVVPAFHAR"
     gene            3725..4312
                     /locus_tag="VLF19_00950"
     CDS             3725..4312
                     /locus_tag="VLF19_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSE91844.1"
                     /translation="MRDPAKPQQIVDAAIRVFARTGYYNSRVSDIAREAGIASGTIYL
                     YFKTKDEILVTLFREKMAEWVAFVRREVATEHDAVAKVRRLVALHFAVLERDPDLAEV
                     VQVELRQGHKFFRGASAHEVSAYFDLIASVLHEGVAAGRFQADLPIKVATKVLFGAMD
                     QVATSWVLGKRGYRLSDAADAVAAIFLKGVSKDAV"
     gene            4302..5075
                     /locus_tag="VLF19_00955"
     CDS             4302..5075
                     /locus_tag="VLF19_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSE91845.1"
                     /translation="MPFETLLLDREESFVVITLNRPPANAISEVLVRELNDAFDGLAD
                     DERVRAVIVTGAGDRIFCAGADLGSAFAGGSVDMFIRFGNAVLRKIERFPKPVIAAVN
                     GHALGGGCEIAMACHFRLLKESARMGQTESNLGIIPGFGGTQRMSRLIGRTKALEFMV
                     LGTQVAATECLALGLVNRLSKDGETLGDAKALARQIARRPPIATRLILEVVDEGLEAP
                     MDRALEVETRAFLKTLRTEDAAEGIQAFFAKREPQFKGR"
     gene            5079..5828
                     /locus_tag="VLF19_00960"
     CDS             5079..5828
                     /locus_tag="VLF19_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE91846.1"
                     /translation="MDLGIRGRVAIVTGGARSLGRADADVLAAEGAKVAILDLNEEGA
                     QEAAKEISATGAVARGWACDVREAAQVREVAGAVERDLGPVDILVNNAGIIYTMGQIK
                     DTSEQDWALNLAVNLTGTFNATKAVFPGMRERRWGRVVCMASIAGLMGGFGQTAYATT
                     KMGLVGFARSVALEGARHNVTCNVIAPGIVGPNAKLSPLYDRMVKRVAMQREGEPEDV
                     ANAVAFLCSERARYITGAVLTVTGGMDLFTF"
     gene            5954..6274
                     /locus_tag="VLF19_00965"
     CDS             5954..6274
                     /locus_tag="VLF19_00965"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSE91847.1"
                     /translation="MPTVSEIFQNMPSRFRPDKASGTNATVQYDISGDGGGTWHAVIK
                     DGTCTVNPGPGQNPNLTLQVGAQDWVDVTTGKQNPQMLFMSGKLKIKGDMGLAMKLGS
                     MFSV"
     gene            6309..7442
                     /locus_tag="VLF19_00970"
     CDS             6309..7442
                     /locus_tag="VLF19_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE91848.1"
                     /translation="MGPERFADIVDRFTNGYTDARAAGVAPDVLARWQEEIDRNARRM
                     KNLALMTFAPSEPRTGPTPRQEVWRTNKSRLYRYASARTHRTPLLFVPNLGISRPYIF
                     DLLPGGSFVEHMTREGFDFYLLDWGVFGPEDNNLSLAHCVSKILPRMARKVLETSGAS
                     ELSVLGYCMGAPLSASFVASHPEVPVRNFINMAGPIDFSKVGLFGLWLDRKHFDVDRF
                     VDTLGAVPADMVKAGFKLLKPTMDLSTNLNLWWNLWNDKYVEGFTALNKWANEYVAFP
                     GEFFREWVRDFYQGNKLFRGELVTGGRPVRLGAISCPIYVVGAREDYIAPPECVRALI
                     DAVSSREKEYVELPGGHISLIAGRGAALHCWPKISGWLAPRSG"
     gene            7500..8060
                     /locus_tag="VLF19_00975"
     CDS             7500..8060
                     /locus_tag="VLF19_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91849.1"
                     /translation="MADATQQLFEMWRRQFEEGAQAWARVMSGGQPPPPAAADPTAFW
                     KPLVDQWVQAWAGAFARTPMTPDVMPQWKQFLDQSIDAWSRALGQAMNTEAFAQMLGR
                     YLDQWLTASAPVRKASDQAVDAALQTLNLASRTQLTAVARQLVELDERVERVEDTLAA
                     IMRRLDEVSRTLARPKAATPAAGEHG"
     gene            8057..8638
                     /locus_tag="VLF19_00980"
     CDS             8057..8638
                     /locus_tag="VLF19_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSE91850.1"
                     /translation="MIGKTIAELAQGDSAEIVRTVSMDDIAGFVEAVGDHNPVHSDPG
                     YAATTTFREPIAPGIFTAGLISAVIGTRLPGPGAIYLSQSLKFTKPVRAGDTITTRVE
                     VLEVMRERNRIRLSTVCRNQRGEDVLMGEAWVMPSRAPITYDGAPSCRPPRLPLALQP
                     WSVAAQALALWGSLALAVLAASLPRSTTSAGRR"
     gene            complement(8616..>8820)
                     /locus_tag="VLF19_00985"
     CDS             complement(8616..>8820)
                     /locus_tag="VLF19_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE91851.1"
                     /translation="NAVAPGFIETEMVSGIPEKVRARLLDQIPLKRFGTAEEVGRAVV
                     YLVSADGDYITGAEVSINGGLLM"
BASE COUNT         1364 a         3017 c         3224 g         1215 t
ORIGIN      
        1 ggctgaccct cgacgacgtc aaggccgccg cccgccgcct ccagggccac gtcacgcgca
       61 ctcccgtcgt cacgtcccag tcgtgggacg acgccacggg ctatcgcgtt ttcttcaagt
      121 gcgagaacct gcagcgggcc ggcgccttca agatccgggg cgcgctcaac aagctgctca
      181 cgctcacgcc ggaggaacgc gcccggggcg tcatcgcctt ctcgtcgggc aaccacgccc
      241 agggggtggc gctggccgcg cgcatgctgg ggacgacggc ggtgatcgtc atgcccaggg
      301 acgcgcccgc gctcaagctc gaggccacgc gcggctacgg cgccgaggtc gtgttctacg
      361 accggcaggc ggacgaccgc gaggcgatcg cgcggcggct cgcggaggag acggggcgga
      421 tgctggtccc gccctacgac gactactcga tcatggccgg gcagggtacg gcggcgctgg
      481 agcttttcga ggaggtgccg tcgctcgacg cgctggtggc gcccgtgggt ggcggcggcc
      541 tcatggccgg ctgcagcacg gtcggccgcg cgctggcccc cggcatgacg atcgtcgggg
      601 tggaggccga gaccgccaac gacacgtggc tgtcgctcga gaagggcgag cgcgtcacga
      661 tcccgccgcc cccgacgatc gccgacggta tccggatcac gtcgccgggc gcgctcacgt
      721 ttcccgtcct ggtccggaac cgggtcgagg tggtgctcgt gagcgacgat gagatccgca
      781 ctgccgtccg cctcctcgct ctgcgcgcgc acctcgtcac ggagccgacg ggcgccgtcg
      841 gcgccgccgc ggcgctcgcg cggcgtatcg gtgtgcgcgc cggcggccgc gtgggcgtgg
      901 tgctctcggg gggcaacgtg gatcccgaga tgctgatcga catcctccgc gcgcgctgac
      961 gggagcccgc tcgcgatccg gacgggccct gccctccgtg gcgagacccg tgttctgtcc
     1021 acggcggatc gtcggcctcc ggcgcgggct cacgcctcgg tctccagccg cgcctcgccg
     1081 aggccgatgc gcgtcgcctc gacgtccagc tcctgctcga tgcggtgcag cacctcgtcg
     1141 ctgatcgccc cctgatcccg cagcgcgatc acggcccggc gttccgcgct gagggtctcg
     1201 tgacggagcc ggcggtagga ggcctggccc tccgcggcca tgcggtcctg gccggcggtg
     1261 aggggggagg agcgccgcag gcgctgggtg tagatcgccc gcaggcggtc cacctgggcc
     1321 gctcgcgccc acggcgcacc cgtgacctcg tcgagccgcg cgagagccgc ccgggccgct
     1381 gcctcccgcg cctggacctc ctccagctcc agcgtggcgt cttcgccgag gttcaggacg
     1441 cggatcagac ggggaaggct gaggccctgc accaccagcg tgctcaggat caccacgaac
     1501 gagagcacgg tgatctcgtc gcgaaacgga aacggggcgc cggaggccgt ggtcagcggc
     1561 agggccagcg cggcggcgag ggagacgatg ccccgcatcc ccgcccagcc gagcaggaac
     1621 agggcctgcc atggcggcat cgggtcgcgc cgccgcagcg aggggctgag gaggcgcgga
     1681 atgactgcgg cgagtgggac ccagacgaga cgcacggcga tggccgtcgc gctgatgacg
     1741 gcccccttga ccaggagcgg cgccagcgtg ccggggggcg cggcctcgcg gatggcggcg
     1801 agctgcaggc cgatgaggat gaagatgacg ccgttcagca cgaagacgag gaggtcccag
     1861 acggcgcgcg cctgcagccg ggtcgcgggc gccacgacct cgctgaagcc ctggcggagg
     1921 tagaggcctc ccacgacaca ggccaggacg gcggaggtgt gcatgcgctc ggcgagcacc
     1981 cacgcggcgt acggcgccag cagcgtgacc gcggtctcgg cgagactgtc cgcggtcagc
     2041 cggagcgccc ggcgggccac ggcgccgacg acgaggccga tcgcggtccc cccgagcgcg
     2101 gtcgcgacca acagccacgg cgtctcgccg agcacgaagc tgccggtgac cacggcggcg
     2161 acggcgacgc ggtagagcac cagcgctgtg gcgtcgttga tgaggctctc gccctcgagg
     2221 atcgtgacga ggcggtgggg aatgtgcagc cgccgtgcga tggccgtcgt ggccacggcg
     2281 tcgggcggcg agacgatcgc gcccagggcg aaggccgcgg gccagctgag gcccgggagg
     2341 atgagccggg ccaccgcggc gaccgcggcc gtggtggcca gcacgagccc gacggcgaga
     2401 agggtgatcg ggcggagatt cgcccggaag tcgcgcagcg aggtgaagta ggccgcggcc
     2461 cataggatcg gcggcaggaa aatgaagaag acgaggtcgg gctggagcgg taccaccggc
     2521 acgccgggca ccaagctgag cgcgaggccc ccgagcacga gcaggatggg gtacgggatg
     2581 aggacacgtt gggcgagcgt gaccaggacg agcacggcga tcaggagcaa gaccaccgtc
     2641 tggagctgat ggagcgtcgc catggccacg agtcgacgca gcctgcggcc gccgctggat
     2701 ctcccctccg ccgcgactca gcgtgcgtgg aaggccggga cgacctcctc ggccaatttc
     2761 gccagctggt cgagcacctg gtcgggtggg accatcggcc gcacgatgaa cttcgaggcg
     2821 ccgctccgca cgtactgctc gagtcgctcg gcgagcaccg cgggcggccc gaacgcggtg
     2881 acggcggcga gggtggcctc gtccgtgcgt ccgcggggaa tgaagggctg cgccgtcgcc
     2941 cgggcccgcg ccgcgtcgtc gtcgaagccg aaatacacga gcgtgccgaa gtggtcggtg
     3001 ggcacctcgc gcccggcctc ggcggcgaag gcctgggtct tctcgatccc gacgcggact
     3061 tgctcgggcc tgatgaacga cgggatccac ccgtcgccca ggcggccggc ccggcgcatc
     3121 gcggcctcgc tgttgccgcc gatccacacg gggaaagggc gctggatggg cttcggcagc
     3181 acgctgacct tctcgagctg ccagaactcg cccgcgaagg tcacctcgtc ctcggcccag
     3241 cagtgccgca tgatggagat ggcctcgtcg gtccgccggc cgcgctcctt gaagggcacg
     3301 cccgcggcgc ggaactcgcg ctcctgctcg acgccgacgc cgagcgcggg cagcatgcgg
     3361 ccacccgaga gccagtcgag catggccagc tcgcgggcga gcagcaccgg cgtccggaac
     3421 ggcgtgatga gcacgctggg cccgaacttg aggcgccggg tgcgcgcggc gaccgcggcc
     3481 atggccgtga ccggctccag caccggcagg ggcgaggaca ggcgctcgtt gaaccacacg
     3541 gagtcgatgt cggtccgctc gcagaggtcg agcaagcgcc acaagtgttc gacgccgccg
     3601 cccgccggcc aggggccggg catcacgccg atccggtact tgatcttcat ggccgcgatc
     3661 ctaacacgcg cgttgacctt tctcccggcg gcccctacaa tgaaccacga ttcagccact
     3721 tcgcatgcgc gacccagcta agccccagca gatcgtcgac gctgccatcc gcgtgttcgc
     3781 gcgcaccggc tactacaact cgcgggtgtc tgacatcgcg cgcgaggccg gcatcgccag
     3841 cgggacgatc tacctgtact tcaagaccaa ggacgagatc ctcgtcaccc tgttccggga
     3901 gaagatggcc gagtgggtag ccttcgtgag gcgggaggtc gccaccgagc acgacgcggt
     3961 ggccaaggtc cggcggctcg tggccctgca cttcgccgtc ctggagcgcg accccgacct
     4021 cgccgaggtc gtgcaggtgg agctgcgcca ggggcacaag ttcttccgcg gcgcttccgc
     4081 tcacgaggtg tcggcctact tcgacctgat cgcctcggtg ctgcacgagg gcgtcgcggc
     4141 cgggcgcttc caggcggatc ttccgatcaa ggtggccacc aaggtactct tcggggcgat
     4201 ggaccaggtg gcgacttcgt gggtgctggg caagcgcggc taccggctga gcgacgcggc
     4261 cgacgccgtg gccgcgatct tcctcaaggg agtgtcgaag gatgccgttt gagacgctgc
     4321 tcctcgaccg cgaggagagc ttcgtggtca tcacgctgaa ccgcccgccc gcgaacgcca
     4381 tcagcgaggt gctggtgcgg gagctgaacg acgccttcga cggtctggcc gacgacgagc
     4441 gcgtgcgggc ggtcatcgtg acgggcgccg gcgaccggat cttctgcgcc ggggcagacc
     4501 tcggctcggc gttcgccggc ggcagcgtgg acatgttcat tcgcttcggc aacgccgtgc
     4561 tgcgcaagat cgagcgcttc ccgaagcccg tgatcgcggc cgtcaacggc cacgccctcg
     4621 gcggcggctg cgagatcgcc atggcctgcc acttccggct cctcaaggag tcggcgcgga
     4681 tggggcagac ggagtccaac ctcggcatca tccccgggtt cggaggcacc cagcgcatgt
     4741 cgcggctcat cgggcgcacg aaggcgctgg agttcatggt cctcggcacg caagtggcgg
     4801 cgacggaatg tctcgcgctg gggctcgtca accggctctc gaaggacggc gagacactcg
     4861 gcgacgccaa ggccctggca cgccagatcg ccaggcgccc gcccatcgcc actcgactca
     4921 tcctcgaggt ggtggacgag gggctcgagg ccccgatgga ccgcgcgctc gaggtcgaga
     4981 cgcgcgcgtt tctgaagacg ctgcgcaccg aggacgccgc cgaggggatc caggcgttct
     5041 tcgccaagcg cgagccgcag ttcaaggggc gctgaggagt ggatctcggc atccgcggtc
     5101 gcgtcgcgat cgtcaccggg ggggctcgca gcctcgggcg cgccgacgcc gatgtgctcg
     5161 ccgccgaggg cgcgaaggtc gccatcctcg acctcaacga ggagggagcg caggaggcgg
     5221 ccaaggagat ctcggccacg ggcgccgtgg cgcgcggctg ggcctgcgac gtccgcgagg
     5281 ccgctcaggt ccgcgaggtg gcgggtgccg tcgagcgcga tctcggcccg gtcgacatcc
     5341 tcgtcaacaa cgccggcatc atctacacga tgggccagat caaggacacg agcgagcagg
     5401 actgggcgct gaacctggcc gtcaacctga ccggcacgtt caacgcgacg aaggcggtgt
     5461 tcccgggcat gcgcgagcgg cgctggggcc gcgtcgtgtg catggcctcg atcgccggac
     5521 tcatgggcgg cttcggacag acggcgtacg cgacgacgaa gatgggactg gtgggcttcg
     5581 cgaggtcggt cgcgctcgag ggggcgcggc acaacgtcac ctgtaacgtg atcgcgcccg
     5641 gcatcgtggg tcccaacgcc aagctgtcgc cgctctacga ccggatggtc aagcgcgtgg
     5701 ccatgcagcg ggaaggggag ccggaggacg tggccaacgc cgtcgccttc ctctgctcgg
     5761 agcgtgcgcg gtacatcacg ggcgcggtgc tcacggtcac cggggggatg gacctgttca
     5821 cgttctagca gcaatcgcgc gccatgcccg accgtcgggc cgcgatgcga gacgcaaacc
     5881 ggcagccgag ccgggcggtg aaacgggcgg ggaggggctg cgaagtcggt tcggacggca
     5941 aaaggaggag atcatgccga cagtcagcga gatcttccag aacatgccca gcaggttccg
     6001 ccccgacaag gccagcggga cgaacgccac cgtccagtac gacatcagcg gggacggagg
     6061 gggcacctgg cacgcggtca tcaaggacgg cacctgcacg gtgaacccgg ggcccgggca
     6121 gaaccccaac ctgacgctgc aggtcggggc gcaggactgg gtcgacgtta cgaccggcaa
     6181 gcagaatccg cagatgctgt tcatgtcggg caagctgaag atcaagggtg acatggggct
     6241 cgcgatgaag ctggggtcga tgttctcggt ctgactcgac gttctcgatg tgagcgagag
     6301 gagcgtggat gggcccggag cgattcgccg acatcgtcga ccggttcacg aacggttaca
     6361 ccgacgcgcg cgccgccggc gtggcgccgg acgtgctcgc gcggtggcag gaagagatcg
     6421 accgcaacgc ccggcgcatg aagaatttgg ccctgatgac gttcgcgccg agcgagccgc
     6481 ggaccggtcc gacgccgcgc caggaggtct ggcgcacgaa caagtctcgc ctctatcgct
     6541 acgcctccgc ccgcacgcac cggacgccgc tgctgttcgt gcccaacctc ggcatcagcc
     6601 gcccctacat cttcgatctc ctgcccggcg gctccttcgt cgagcacatg acccgcgagg
     6661 gcttcgattt ctacctgctc gactggggcg tcttcggccc ggaggacaac aacctcagcc
     6721 tcgcgcactg cgtgtcgaag atcctgccca ggatggcccg gaaggtgctc gagacctcgg
     6781 gcgcatcgga actctcggtg ctcggctact gcatgggggc gccgctgtcg gcgtccttcg
     6841 tcgccagcca ccccgaggtg cccgtacgga acttcatcaa catggcgggg ccgatcgact
     6901 tctcgaaggt cgggctgttc gggctctggc tcgaccgaaa gcacttcgac gtcgaccgct
     6961 tcgtggacac gctcggcgcg gtgccggccg acatggtgaa ggccggcttc aagctgctca
     7021 agccgaccat ggacctgtcc acgaacctca acctctggtg gaacctctgg aacgacaagt
     7081 acgtcgaggg gttcacggcg ctcaacaagt gggccaacga gtacgtggcc ttccccggcg
     7141 agttcttccg cgagtgggtg cgcgacttct atcagggcaa caagctcttc cgcggggagc
     7201 tggtgacggg cggccgtccc gtgcggctcg gcgccatcag ctgcccgatc tacgtcgtcg
     7261 gcgccaggga ggactacatc gccccgccgg agtgcgtgcg agcgctcatc gatgccgtgt
     7321 cgagccggga gaaggagtac gtggagctgc ccggcggcca catctcgctc atcgcgggcc
     7381 gcggcgcggc gcttcactgc tggcccaaga tatccgggtg gctcgcgccc cgctccggtt
     7441 gaggcgccgc gtcatcgggg ttggcgcaaa gcgtcaatcc ggataggatg gaagcagcca
     7501 tggctgacgc gacccagcaa ctgttcgaga tgtggcgccg gcagttcgag gagggcgccc
     7561 aggcgtgggc cagggtgatg agcggcggcc agccgcctcc gccggcagcg gcggacccca
     7621 cggcgttctg gaagcccctc gtcgaccagt gggtgcaggc gtgggcgggc gccttcgcgc
     7681 ggactcccat gacgccggac gtcatgccgc agtggaagca gttcctcgac cagtcgatcg
     7741 acgcctggtc acgggccctc gggcaggcca tgaacaccga ggccttcgcg cagatgctgg
     7801 ggcgatacct ggaccagtgg ctcaccgcct ccgcgcccgt gcgcaaggcg agcgaccagg
     7861 ccgtggacgc cgcgctccag acgctcaacc tcgcctcgcg cacgcagctc acggccgtgg
     7921 cccggcagct ggtcgagctg gacgagcgcg tcgagcgcgt cgaggacacc ctcgcggcga
     7981 tcatgcggcg cctggacgag gtgtcccgga cgctcgcgcg gccgaaggcg gcgacgccgg
     8041 cggccgggga gcacggatga tcggcaagac gatcgcggag ctggcgcagg gcgactccgc
     8101 ggagatcgtg cgcacggtgt cgatggacga catcgcaggg ttcgtggagg cggtcggcga
     8161 ccacaatccc gttcacagcg acccgggcta cgcggcgacg acgaccttcc gggagccgat
     8221 cgcgccgggc atcttcaccg cggggctgat ctcggcggtc atcggcacca ggctgccggg
     8281 accgggcgcg atctacctgt cgcagagcct caagttcacc aagcccgtga gagccggtga
     8341 cacgatcacc acgcgcgtcg aggtgctcga ggtgatgcgc gagcgcaacc gcatccggct
     8401 ctcgacggtg tgccgtaacc agcgggggga ggacgtgctg atgggcgagg cgtgggtgat
     8461 gccctcgcgc gcgccgatca cctacgacgg cgcgccgtcc tgccgcccgc cgcgtctgcc
     8521 cctcgctctg cagccgtggt cggtcgccgc gcaggccctg gcgctgtggg ggagcctggc
     8581 gctggcggtg ctcgcggcga gcctgccgcg ctcgactaca tcagcaggcc gccgttgatg
     8641 gagacctcgg cgcccgtgat gtagtctccg tcggccgaca cgaggtagac cacggcgcgc
     8701 ccgacctctt cggccgtccc gaaccgcttg agggggatct gatcgagcag cctggcgcgc
     8761 accttctcgg ggatgcccga gaccatctcc gtctcgatga atcccggcgc caccgcgttc
//
LOCUS       DATIAZ010000028         4658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_137223, whole genome shotgun sequence.
ACCESSION   DATIAZ010000028 DATIAZ010000000
VERSION     DATIAZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137223"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VLF19_00990"
     CDS             complement(<1..306)
                     /locus_tag="VLF19_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSE91852.1"
                     /translation="MKLVVIGGGIAGLACAHRAVELARERGLALDLTLLEARDRLGGT
                     IETERAGGFLVEAGPDSFLSEKPWALALCRRLGVEHRLARTDDRHRRTFVWFRGRLHP
                     "
     gene            complement(303..1229)
                     /gene="hemH"
                     /locus_tag="VLF19_00995"
     CDS             complement(303..1229)
                     /gene="hemH"
                     /locus_tag="VLF19_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HSE91853.1"
                     /translation="MIDSVLLVAFGGPTAPGEIRPFLENVTRGRRIPPERIEEVARHY
                     ERMPGGRSPLNELTLIQARSLEALLAGANVRLPVFVGMRNWTPYLHETLAAMSRRGRR
                     RALGVILSAFRTEASWERYMEDVTVARARTPQAPDVVFAPPFFDHPLFVEAVADRVRA
                     ALGRVPSPARAAAPVVFTAHSVPTAMADASPYVGDFTTTSRLVAERLGLVRWSRAFQS
                     RSGNPRDPWLEPDVNDVVKDLASQGVRHVVVAPIGFVCDHVEVLYDLDVEARATAAAA
                     GLTLHRATAVNDHPRFIAALADLVQRAQPTAA"
     gene            complement(1226..2260)
                     /gene="hemE"
                     /locus_tag="VLF19_01000"
     CDS             complement(1226..2260)
                     /gene="hemE"
                     /locus_tag="VLF19_01000"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSE91854.1"
                     /translation="MPADASPLLLAARRRPAPYTPVWLMRQAGRYLPEYRAMRARFGF
                     LELCHNPEAAAEVTLQPVERFGVDAAILFADILLIVEPLDVALEFTRGEGPVIHRPVR
                     TAADVARLGPIDVEGGVPFVFETARTVARALAGRVPLIGFAGAPFTVASYLVEGGPSR
                     DYLAVKGLMYESPEAWHRLLALLADATARYLNGQIAAGAEVVQLFDSWVGVLSPADYR
                     AFVLPHVRTLVNALAPGVPVIHFGTGTAALLPLMREAGGDVIGLDWRVDLDAGWAAVG
                     HDVAVQGNLDPAVLLAPRPMIRDRVKDILARAGGRPGHIFNLGHGVHQQTPVENVKAL
                     VDMVHELSGR"
     gene            2325..3995
                     /locus_tag="VLF19_01005"
     CDS             2325..3995
                     /locus_tag="VLF19_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSE91855.1"
                     /translation="MSFISGKQAFLQILQSEGVSVMFGNPGTTELPLMDGLARQPDIR
                     YVLALQEAAAIAMADGYAQASGGLAAVNVHVSPGLGNAMGMLYDAQKAGAPLLLTAGQ
                     HDQSFTVTEPILWSELPPIAQPFVKWSTEARRLEDLPRIVRRAVKTALAQPTGPVFLS
                     LPVDVLNAERDVDLLESTRVAPRIVGDRQAVDRAAVLLAKAERPLIVAGDAVTHSDAL
                     AELIELAELVGCPVVAESVPSTCSFPFTHPQYAGAMPRLGPLIRAVLMRHDLVFSVGG
                     DLFTLSLPDDVDPMPPGVTVVHLDVNPWELGKNYPAAVAIQGDPKATLPELSEALRRH
                     TTKTGHPDAGKRREAARAAHERMRAELAERARKDAGQVPMSPLALVHAVAETVPADAT
                     IIDETISSANGVRQLFRCADAKSFFGLRGGGIGWGLPAALGVKLALPHRPVVALVGDG
                     SAMYTAQALWTAAHESLAVVYVIFNNASYRILKQRTLALKGFSAQDDVYVGMDLERPL
                     IDYVGLARSLGVPGERVEKTGDVSVALERGLRNGGPYLVDVRIDPSFK"
     gene            4151..>4658
                     /locus_tag="VLF19_01010"
     CDS             4151..>4658
                     /locus_tag="VLF19_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE91856.1"
                     /translation="MAMTFGIHIGHMGGPLAEMRRLWKFAEAQGFDWFSVSDHFQESP
                     PRGGDLDCFEAISIMTAAAVETQRIRIGSLVYSVLYRNPGVLASALTSIDHLSGGRVD
                     CGIGAAWHEIESGCFGIPFPSIGIREDMLEEYAQVLRLLFDPSVKRADFTGKHFSLKN
                     APNNPKPLQ"
BASE COUNT          663 a         1700 c         1671 g          624 t
ORIGIN      
        1 gggatgcagc cgcccgcgga accagacgaa cgtccggcga tggcggtcgt cggtgcgcgc
       61 gaggcgatgc tcgacgccga ggcggcggca gagcgccagc gcccagggct tctcggagag
      121 gaaggagtcg gggccggcct cgacgaggaa gccgccggcg cgctcggtct cgatcgtgcc
      181 gccgagccgg tcgcgcgcct cgagcagcgt caagtcgagg gccaggccgc gctcgcgcgc
      241 cagctcgacg gcgcggtggg cgcaggcaag cccggcgatg ccgccgccga tcacgacgag
      301 cttcacgcgg ccgtcggctg cgcgcgctgc accagatcgg ccagggccgc gatgaaccgc
      361 gggtggtcgt tcacggccgt ggcgcggtgg agcgtgaggc cggcggcggc cgcggtggcc
      421 cgcgcctcga cgtcgaggtc gtagagcacc tcgacgtggt cgcagacgaa tccgatcggc
      481 gcgacgacga cgtggcgcac gccctgcgag gcgaggtcct tgacgacgtc gttcacgtcg
      541 ggctcgagcc aggggtcacg cgggttgccg ctgcggctct ggaacgcccg cgaccagcgc
      601 acgagtccga gccgctcggc cacgagccgc gacgtggtcg tgaaatcgcc cacgtacggc
      661 gaggcgtcgg ccatcgccgt cggcacgctg tgcgccgtga agaccacggg cgccgccgcc
      721 cgggccgggg acggcacgcg gccgagcgcc gcccgcacgc ggtcggcgac ggcctcgacg
      781 aagagcggat ggtcgaagaa cggcggggcg aagaccacgt ccggcgcctg gggcgtccgc
      841 gcgcgggcca ccgtcacgtc ttccatgtag cgctcccacg aggcctccgt gcggaacgcc
      901 gagaggatca cccccagcgc gcgccgccgc ccgcgccggc tcatcgcggc cagcgtctcg
      961 tgcaggtagg gcgtccagtt gcgcatgccc acgaagaccg gcagccggac gttggcgccg
     1021 gcgagcaggg cctcgagcga gcgcgcctgg atcagcgtca gctcgttgag gggcgagcgg
     1081 cctcccggca ttcgctcgta gtggcgcgcg acctcctcga tccgctcggg cggaatccgc
     1141 cggccgcgcg tgacgttctc gaggaagggc cggatctccc cgggcgccgt gggcccgccg
     1201 aacgcgacga gcagcaccga gtcgatcacc ggccgctcag ctcgtggacc atgtccacca
     1261 gcgccttcac gttctcgacc ggcgtctgct ggtgcacgcc gtgcccgagg ttgaagatat
     1321 gcccgggccg gcccccggcc cgggccagga tgtccttgac ccggtcgcga atcatcggcc
     1381 gcggcgccag gagcaccgcc gggtcgagat tgccctgcac ggccacgtcg tggccgacgg
     1441 cggcccagcc cgcgtcgaga tccacgcgcc agtcgagccc gatcacgtcc ccgcccgcct
     1501 cacgcatgag cggcagcaac gccgcggtgc cggtcccgaa gtggatcacg ggcacgcccg
     1561 gcgcgagcgc gttcacgagc gtgcggacgt gggggagcac gaatgcgcgg tagtccgccg
     1621 gcgagagcac ccccacccac gagtcgaaga gctgtacgac ctcggcgccc gcggcgatct
     1681 ggccgttcag gtagcgcgcg gtcgcgtcgg ccagcagcgc cagcagccgg tgccacgcct
     1741 ccggcgactc gtacatgagc cccttgacgg cgagatagtc gcgcgacggg cctccctcga
     1801 cgagatacga ggccacggtg aagggcgcgc ccgcgaaccc gatcagcggc acgcggcccg
     1861 cgagcgcgcg cgcgacggtc ctggccgtct cgaagacgaa cggcaccccg ccctcgacgt
     1921 cgatcggacc cagacgggcg acgtcggcgg ccgtccgcac cggccggtgg atcaccgggc
     1981 cctcgcccct ggtgaactcg agcgccacgt cgagcggctc gacgatcagc aggatgtcgg
     2041 cgaagaggat ggccgcgtcc acgccgaacc gctcgacggg ctgcagcgtc acctctgccg
     2101 cggcctcggg gttgtggcac agctccagga agccgaagcg cgcccgcatg gcccggtact
     2161 cgggcaggta gcgcccggcc tggcgcatga gccacaccgg cgtgtagggc gcggggcggc
     2221 ggcgcgcggc gaggaggagc ggtgatgcat cggcgggcac ggggagagcg taccggcccg
     2281 gcccctctgg aatcaagcgc gccgcgggtt tatgatccag ccccatgtca ttcatatccg
     2341 gcaagcaggc gttcttgcag atcctccagt ccgaaggcgt ctcggtcatg ttcggcaacc
     2401 ccggcaccac ggagctgccg ctcatggacg gcctcgcacg ccagcccgac atccgctacg
     2461 tgctcgcgct gcaggaggcc gcggccatcg cgatggccga tggctatgcg caggccagcg
     2521 gcggactcgc cgccgtcaac gtgcacgtct cgcccgggct cggcaacgcc atgggcatgc
     2581 tctacgatgc gcagaaggcc ggcgcgccgc tcctgctcac ggccggccag cacgaccagt
     2641 cgttcacggt gaccgagccg attctctggt ccgagctgcc gccgatcgcc cagccgttcg
     2701 tgaagtggtc gacggaggcg cggcgcctcg aggatctccc ccgcatcgtc cgccgcgcgg
     2761 tgaagacggc gctcgcgcag cccacgggcc ccgtgttcct ctcgttgccg gtggacgtgc
     2821 tcaacgccga gcgggacgtc gacctgctgg agtcgacgcg cgtggccccg cgcatcgtcg
     2881 gcgaccgcca ggcggtcgac cgcgccgccg tgctgctggc gaaggccgag cggcccctca
     2941 tcgtcgccgg ggacgccgtg acccacagcg atgcgctcgc cgagctgatc gagctggcgg
     3001 agctggtcgg ctgtcccgtc gtcgccgagt ccgtgccctc cacgtgcagc ttcccgttca
     3061 cccaccccca gtatgcgggc gcgatgccgc gcctcggccc gctcatccgc gcggtgctca
     3121 tgcgccacga cctcgtcttc tccgtgggcg gcgacctctt cacgctctcc ctgcccgacg
     3181 acgtcgatcc catgccgccg ggcgtgaccg tggtgcacct cgacgtgaac ccgtgggagc
     3241 tgggcaagaa ctaccccgcc gccgtggcca tccagggcga tccgaaggcc acgctgccgg
     3301 agctgtccga ggcgctccgg cggcacacga ccaagaccgg ccatcctgac gccggcaaac
     3361 ggcgcgaggc cgcgcgggcc gctcacgagc ggatgcgcgc ggagctcgcg gagcgggcgc
     3421 ggaaggatgc cggccaggtg cccatgtcgc cgctcgcgct ggtccacgcc gtcgccgaga
     3481 ccgtccctgc agacgccacg atcatcgacg agacgatctc gtccgccaac ggcgtgcgcc
     3541 agctcttccg ctgcgccgac gccaagagct tcttcggcct gcgcggaggc gggatcggct
     3601 ggggcctgcc cgcggcgctc ggcgtgaagc tggcgctgcc gcaccgcccc gtggtcgccc
     3661 tggtcggcga cggcagcgcg atgtacacgg cccaggcgct gtggacggcc gcgcacgagt
     3721 cgctggccgt ggtctacgtc atcttcaaca acgcgtcgta ccgcatcctc aagcagcgga
     3781 cgctcgccct caagggcttc tcggcgcagg acgacgtcta cgtgggcatg gacctcgagc
     3841 gcccgctgat cgactacgtc ggcctcgcgc gctcgctcgg cgtgcccggg gagcgcgtgg
     3901 agaagaccgg ggatgtctcc gtcgcgctcg agcgcgggct ccggaacggc ggcccctatc
     3961 tcgtcgacgt ccgcatcgac ccgtcgttca agtaggcctc tcaccgtccg gaccagagga
     4021 gggtttcaga gatgaggcct ttccggctcc cccgctggcg gttcacgagc tgctgtgggc
     4081 attcgtgggg tcaccgcccc ggcgccggcg ctcgaacatg ggtcgagcac tgggcgtgag
     4141 gagtgtcggg atggcgatga cattcgggat ccatatcggg cacatggggg ggccgctcgc
     4201 ggagatgcgg cggctgtgga agttcgcgga ggcgcagggc ttcgactggt tctcggtgtc
     4261 cgatcacttc caggagtcgc cgccgcgggg aggcgatctg gactgcttcg aggccatctc
     4321 gatcatgacg gcggcggccg tggagacgca gcggatccgg atcggctcgc tggtctactc
     4381 cgtgctctac cgcaatccgg gcgtgctcgc ctcggccctg acgtcgatcg accacctctc
     4441 gggcgggcgc gtggactgcg gcatcggcgc cgcgtggcac gagatcgaga gcggctgctt
     4501 cggcatcccc ttcccgtcca tcggcatccg cgaggacatg ctggaggaat acgcgcaggt
     4561 cctgcgcctc ctcttcgacc cctccgtgaa gcgcgccgac ttcacgggca agcacttcag
     4621 cctcaagaac gccccgaaca atcccaagcc gctgcagc
//
LOCUS       DATIAZ010000029         4322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1321881, whole genome shotgun sequence.
ACCESSION   DATIAZ010000029 DATIAZ010000000
VERSION     DATIAZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321881"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VLF19_01015"
     CDS             complement(<1..452)
                     /locus_tag="VLF19_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSE91857.1"
                     /translation="MNASVSVNGRTYRSPAAPTVVVCIDGSEPDYFDRAIEAGAAPAV
                     ARFRREGFATLARSVVPSFTNPNNLSIVTGVPPAVHGIAGNYFYDATTGREVMMNDPR
                     YLRCGTMLAALAQVGARVAVVTAKDKLRRLLGHEMTGVCFSSEKADQAT"
     gene            complement(457..1110)
                     /locus_tag="VLF19_01020"
     CDS             complement(457..1110)
                     /locus_tag="VLF19_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HSE91858.1"
                     /translation="MARGAVVGLKAAVWAACLAPLAALGVWVATGDLTANPISFVTNH
                     LGDWTFRMLLASLAMTPLRLVTGWGWPALLRRLLGLFAFAYALLHFAIWILLDHFFNW
                     GQMAEDIVKRPYITVGMLALLGLVPLAATSTSAMIKRLGARNWRRLHRLVYAVGVLAA
                     LHFLWLAKVGRTDQYVHAAVLALLLAVRAADALRRLARRRREAKIPVVGRSAGSSIP"
     gene            complement(1115..2071)
                     /gene="msrP"
                     /locus_tag="VLF19_01025"
     CDS             complement(1115..2071)
                     /gene="msrP"
                     /locus_tag="VLF19_01025"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HSE91859.1"
                     /translation="MLIRRAERFKATEITDEGLVLDRRAFIAGAAAALAGAACDVNAA
                     QAPSQGAALPAPRNDALSVKQSPTTWESATTYNNFYEFGVDKDDPAREAHRLKTRPWT
                     VKVDGLVHKPKTFDIDEILKAFPLEERVYLLRCVEAWSMVIPWVGFPLAALLKRVEPT
                     GAAKYVEFTTLHDPQQMPGQKPGWFGGGLDWPYTEGLRLDEALHPLTLMTVGMYGRVL
                     PNQNGAPIRVVVPWKYGFKSAKSIVRIRLAADQPGTAWNKAAPHEYGFYSNVNPNVSH
                     PRWSQATERRIGEFRRRETLMFNGYGEQVASLYAGMDLKKNY"
     gene            complement(2096..2776)
                     /locus_tag="VLF19_01030"
     CDS             complement(2096..2776)
                     /locus_tag="VLF19_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSE91860.1"
                     /translation="MPTILVVDDEPEIVRGLEDNLRYEGYQTFSATDGEQALALAARE
                     APDLIVLDLMMPRLSGWDVCRALRARGIDVPIIMLTARGGEADRVRGLELGADDYLAK
                     PFSLRELLARVRAVLRRPGPRLKVEEYAFGDVRVRLRGRQVFRAGREVAVTRKEFDLL
                     VHLLGHRGEVLTRERLLEEVWGYERFPTTRTVDTHVLRLRRKFETDPDRPTWILTVHG
                     QGYRFATA"
     gene            complement(2776..3069)
                     /locus_tag="VLF19_01035"
     CDS             complement(2776..3069)
                     /locus_tag="VLF19_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91861.1"
                     /translation="MIAPSAPCAPLDIDPRAALLHDRARLEAHVAELVAPSREYLEQE
                     LRREDSPACAVLDDCGVRRPSLDTSAVGRLDGRALAAATVQAGAAVVAATGSG"
     gene            complement(3152..3931)
                     /locus_tag="VLF19_01040"
     CDS             complement(3152..3931)
                     /locus_tag="VLF19_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSE91862.1"
                     /translation="MGRPRVSIVIPVRNDAAALARTLDWLERELTIRGGGADPAAGAE
                     TIVSASGDPDGTARAVGGRARLLWPGGSTRAALMNAGAAAADGDVLFFLHADSFPPRA
                     ALALITRALEDPRAVGGAFEHRFAEPIGSLRAITWINRIRYRLTRNYYGDQGIFVRAS
                     IFRELGGYRDLRLMEDLDLSQRLKRRGRHVLVRTPLVTSGRRFLARGPWRTFFFIVWL
                     LLLHTLRIDTQRYAERWRGPADRPPGSPWPGQTGDVASARR"
     gene            complement(3935..>4322)
                     /locus_tag="VLF19_01045"
     CDS             complement(3935..>4322)
                     /locus_tag="VLF19_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HSE91863.1"
                     /translation="PWPPFGAGDPRRALAIEPIDPRIHFALNCGARSCPPVGVYRAAA
                     LDRQLAVAARNFLNQEVGLDDRGRVRCSRLLKWYGTDFGPREALRDFLLAHLDDGPAR
                     RALAASRAACQVFDAYSWALARPPVE"
BASE COUNT          630 a         1529 c         1596 g          567 t
ORIGIN      
        1 gtcgcctggt cggccttctc cgacgagaag cagacgccgg tcatctcgtg gccgagcagc
       61 cggcgcagct tgtccttggc ggtgacgacg gccacgcgtg cgccgacctg agcgagggcg
      121 gccagcatcg tgccgcagcg caggtagcgc gggtcgttca tcatgacctc acggccggtc
      181 gtcgcgtcgt agaagtaatt gccggcgatg ccgtgcaccg cgggcggcac gccggtcacg
      241 atcgagaggt tgttcggatt cgtgaagctc ggcaccacgc tccgcgcgag cgtcgcgaac
      301 ccctcccggc gaaagcgcgc gacggcgggc gccgcgccag cctcgatcgc gcggtcgaag
      361 tagtcgggct cgctgccgtc gatgcacacg acgacggtcg gggcggccgg gctgcgatac
      421 gtgcggccgt tgacggagac ggacgcgttc atggactcac ggaatactac tcccggcaga
      481 ccggccgacg acgggaattt tcgcctcgcg ccggcgtcgt gccagacgac gcagggcgtc
      541 cgccgcgcgc acggcgagga gcagggcgag gacggcggcg tggacgtact ggtcggtgcg
      601 cccgaccttc gccagccaca ggaagtgcag cgccgccagc acgccgaccg cgtacaccag
      661 gcgatgcagg cgcctccagt tccgggcccc caggcgcttg atcatcgcgc tcgtggaggt
      721 cgccgccagc ggcaccaggc ccaggagcgc cagcatcccg acggtgatgt acggacgctt
      781 cacgatgtcc tccgccatct ggccccagtt gaagaagtga tcgagcagga tccagatggc
      841 gaaatggagc agagcgtagg cgaacgcgaa caggccgagc agcctccgca gcagcgccgg
      901 ccacccccag cccgtcacga ggcgcagcgg cgtcatggcc aggctggcga ggagcatcct
      961 gaacgtccag tcgcccaggt ggttcgtgac gaagctgatg gggttggcgg tgaggtcgcc
     1021 ggtggcgacc cagacgccga gcgccgcgag cggcgccagg caggccgccc agaccgccgc
     1081 cttcaagccg acgacggcgc cgcgcgccac gacctcagta gttcttcttc agatccatcc
     1141 ccgcgtagag gctcgcgacc tgctcgccgt agccgttgaa catgagcgtc tcgcggcggc
     1201 ggaactcgcc gatccgccgc tccgtcgcct ggctccagcg cgggtggctg acgttcgggt
     1261 tgacgttcga gtagaacccg tattcatggg gcgccgcctt gttccatgcc gtccctggct
     1321 gatcggccgc gagccggatg cggacgatcg acttcgcgct cttgaagccg tacttccacg
     1381 gcacgacgac acggatgggc gcgccgttct ggttgggcag cacgcggccg tacatgccca
     1441 cggtcatcag ggtcagcggg tgcagcgcct cgtcgaggcg caagccctcc gtgtacggcc
     1501 agtcgagccc gccgccgaac cagcccggct tctggcccgg catctgctga gggtcgtgca
     1561 gggtcgtgaa ctcgacgtat ttcgcggcgc cggtcggctc cacgcgcttg agcagggcgg
     1621 ccagcgggaa gccgacccag gggatgacca tcgaccaggc ctcgacacag cggagcaggt
     1681 acacgcgctc ctcgagcggg aacgccttga ggatctcgtc gatgtcgaac gtcttcggct
     1741 tgtggacgag gccgtccacc ttcaccgtcc acggccgcgt cttgaggcga tgggcctcgc
     1801 gcgcggggtc gtccttgtcc acgccgaact cgtagaagtt gttgtacgtg gtcgccgact
     1861 cccacgtggt cggcgactgc ttcacgctga gcgcgtcgtt gcgcggggcc ggcagcgccg
     1921 ccccctgcga gggggcctgg gcggcgttca cgtcgcaggc ggcaccggcc agggccgccg
     1981 cggcgccggc gatgaacgcg cggcggtcga ggaccagccc ctcgtcggtg atctcggtcg
     2041 ccttgaagcg ctcggctcgt cggatcagca tgacgcctcc actatcgcac gggattcacg
     2101 cggtggcgaa ccgatacccc tgcccgtgga ccgtgaggat ccacgtcggt cggtccgggt
     2161 cggtctcgaa cttgcgccgc aggcgcagca cgtgggtgtc cacggtgcgc gtcgtcggga
     2221 accgctcgta gccccacacc tcctccagca ggcgctcgcg cgtgaggacc tcgccgcggt
     2281 ggccgagcag gtggaccagg aggtcgaact ccttgcgcgt cacggcgact tcgcgcccgg
     2341 cccggaacac ctggcggccc cgcaggcgca cccgcacgtc accgaacgcg tactcctcga
     2401 ccttcaggcg gggccccgga cggcgcagca cggcccgcac gcgtgccagc agctcgcgca
     2461 gcgagaaggg cttggcgagg tagtcgtcgg caccgagctc gaggccgcgc acgcggtcgg
     2521 cctctcctcc gcgcgcggtc agcatgatga tgggcacgtc gatgccccgc gcgcggagcg
     2581 cgcggcacac gtcccagccc gagaggcgcg gcatcatgag gtcgagcacg atcagatcgg
     2641 gtgcctcccg cgccgcgagc gcgagcgcct gctcgccgtc ggtcgcgctg aacgtctggt
     2701 agccctcgta ccgcaggttg tcctcgagcc cacggacgat ctccggctcg tcgtccacca
     2761 cgaggatcgt cggcatcacc cgctccccgt ggccgcgacg acggccgcgc cagcctggac
     2821 cgtcgcggcg gcgagcgccc ggccgtcgag ccttccgacg gcgctcgtgt cgagcgacgg
     2881 ccgccggacg ccgcagtcgt cgaggacggc gcaggcaggg ctgtcctcgc gccgaagctc
     2941 ctgctcgagg tactcacggg agggcgccac gagctcggcc acgtgcgcct ccagccgggc
     3001 gcgatcatgg agcaaggctg cccgcgggtc gatgtcgagg ggcgcgcacg gcgcggaagg
     3061 cgcgatcact ccgtgtcccc ctcgagcggg cggggcaccc cggcacggcc ggcgaagccg
     3121 atggcgcggg tgatgaggac gggtaccgcg ctcaccgccg cgcgctcgcc acgtcgccgg
     3181 tctggccggg ccacgggctt cccggcggcc ggtccgcggg cccgcgccac cgctcggcgt
     3241 agcgctgggt gtcgatccgg agcgtgtgga gcaggagcag ccagacgatg aagaagaacg
     3301 tgcgccacgg cccgcgggcg aggaagcggc gccccgatgt cacgagcggc gtgcgcacga
     3361 ggacgtggcg cccccggcgc ttgagccgct ggctgaggtc gaggtcttcc atcaggcgca
     3421 ggtcgcggta gccgccgagc tcgcggaaga tcgacgcgcg cacgaagatg ccctgatcgc
     3481 cgtagtagtt gcgcgtgagg cggtagcgga tgcgattgat ccaggtgatc gcgcggaggc
     3541 tcccgatcgg ctccgcgaag cggtgctcga aggccccgcc gacggcgcgc gggtcctcga
     3601 gcgcccgggt gatgagggcg agcgcggcgc gtggcggaaa cgagtcggcg tgcaggaaga
     3661 acagcacgtc gccgtccgcc gccgccgcgc ccgcgttcat gagcgccgcg cgcgtcgagc
     3721 cgccgggcca gagcaggcgc gcgcggccgc cgacggcgcg cgcggtgccg tcggggtcgc
     3781 cggaggcgga gacgatcgtc tcggcgccag cggcgggatc ggcaccgccg ccacggatcg
     3841 tcagctcccg ctcgagccag tcgagcgtgc gcgccagcgc cgccgcgtcg ttgcggacgg
     3901 ggatgacgat cgacacgcgc ggccggccca ctcgtcactc caccgggggg cgggcgagcg
     3961 cccacgagta cgcgtcgaat acctggcacg cggcgcggct cgccgccagc gcgcggcggg
     4021 cgggaccgtc gtcgaggtgc gcgagcaaga aatcgcgcag cgcctcgcgc gggccgaagt
     4081 cggtcccgta ccacttgagc agcctcgagc accggacccg cccgcggtca tccagcccga
     4141 cctcctggtt caggaagttg cgggcggcga cggcgagctg gcggtcgagg gccgcggcgc
     4201 ggtagacccc gacgggcgga cacgagcgag cgccgcagtt cagcgcgaag tggatgcgcg
     4261 ggtcgatggg ctcgatcgcc agggcccgcc gtggatcgcc ggcaccgaag gggggccacg
     4321 gc
//
LOCUS       DATIAZ010000030         3945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_6126, whole genome shotgun sequence.
ACCESSION   DATIAZ010000030 DATIAZ010000000
VERSION     DATIAZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6126"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VLF19_01050"
     CDS             complement(<1..287)
                     /locus_tag="VLF19_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HSE91864.1"
                     /translation="MEAILDAIVRNTREELGRRRAATTQSALEARCRVLPPARDFEEA
                     LRPSVGGVRLVAEVKKASPSRGVLAPSLDPVELAARYARHGADAISVLTDE"
     gene            complement(323..1534)
                     /locus_tag="VLF19_01055"
     CDS             complement(323..1534)
                     /locus_tag="VLF19_01055"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HSE91865.1"
                     /translation="MTTPKRPFHTASDAEIKSGEVSDVYFARTVEILTHRRDRKRVKA
                     EVYLKSLPEDWQWGVLAGIQEVAALLEGVPVDARAMDEGTLFAPYQPVLVVEGTYVEW
                     AKYETALLGLLCQASGIATKAARCKKAAGERQVISFGARRMHPTLAPMIERNAFIGGC
                     DGVAVTKSAELIDADPMGTIPHSLVLMMGDTVEALRAFHEVVDSKVRRVALIDTLQDE
                     KFEALRVAEALGKDLYAVRLDTPSSRRGDFFRIIQEVRWELDLRGFEHVKMIASGGVD
                     EYEILHLNPIADAYGVGTSIANAPVLNFALDIMEIEGRPIAKRGKWSGAKDVLRCRGC
                     RATEVVPAGRAPRPCACGGEWEPLLKPLVRAGRVVRDLPPPRTIRDYVLDQLTVVPLE
                     TLRRQGLRTDF"
     gene            complement(1686..2564)
                     /gene="galU"
                     /locus_tag="VLF19_01060"
     CDS             complement(1686..2564)
                     /gene="galU"
                     /locus_tag="VLF19_01060"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HSE91866.1"
                     /translation="MTVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPTIQYVVEEA
                     VASGISEIIIVTGRNKRAIEDHFDAAFELECYLNDRGKVDELAQIKTISELASLCYVR
                     QKEPLGLGHAILCAQPLVCDQPFAVFLGDDIIGGAPVPCMRQLLDVYERLDGPVIAVE
                     RVPRARIHQYGVIDARPVQGADGARVYEIRDLVEKPRAEEAPSDLAIIGRYVLTPDLF
                     DILAETRPDRRGEIQLTDALRRLRAQRPMYAVEFEGKRYDTGDKLGFLKATVEFALAR
                     PDLADEFRAYLKSLSI"
     gene            complement(2605..3108)
                     /locus_tag="VLF19_01065"
     CDS             complement(2605..3108)
                     /locus_tag="VLF19_01065"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HSE91867.1"
                     /translation="MTRRRLRLGARLHALLHLDDPPRKIAWALAVGVFIGCTPFWGLQ
                     TVLSIAVAWIFGLNRAATVTGTWLNLPWVAPIVYGASLKIGGLLVPDPEGLRAAWLDY
                     LLEHWRSVSWHDALALFRELSVALLVGCAVVGAVAALGAYAVSLAVLSVRRTRRSGDA
                     RRPRRAA"
     gene            complement(3307..>3945)
                     /locus_tag="VLF19_01070"
     CDS             complement(3307..>3945)
                     /locus_tag="VLF19_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE91868.1"
                     /translation="ILDWLGAGRGRRLLDVGAAEGLLSRPLTEQGWRVTAIEVDPEAA
                     RAGAGHCERMIVTSAGRPLPDLGGPFDVIVCADVLEHLADPESALERLVTHLAPGGAV
                     VVSIPNVAHLWVRLGLLAGRFDYADRGLLDRTHLRFFTRRTLEALLATAALAAERFTA
                     TPVPLYQVVPSRWHGRGLAAVHTLSAFVARSMPCLFAYQFVVLARLARRHAL"
BASE COUNT          610 a         1369 c         1425 g          541 t
ORIGIN      
        1 ttctcgtcgg tgagcaccga gatcgcgtcg gcgccgtggc gggcgtagcg cgccgccagc
       61 tcgaccgggt ccagggaggg agccagcacg ccccgcgagg gcgacgcctt cttcacctcg
      121 gcgacgagcc ggacgccccc caccgacggc cggagcgcct cctcgaagtc gcgcgcgggc
      181 gggagcacgc ggcagcgggc ctcgagcgcg gactgcgtcg tggccgcgcg ccggcggccg
      241 agctcctcac gcgtgttccg cacgatggcg tcgaggatcg cttccatgtc agtgtcgatc
      301 cggcgggggg cctcggagcc gttcagaagt ccgtccgaag cccctggcgg cgcagcgtct
      361 cgagggggac caccgtgagc tgatcgagga cgtagtcgcg gatcgtgcgc ggcggcggga
      421 ggtcgcgcac gacgcggccg gcccgcacca gcggcttcaa cagcggctcc cactcgccgc
      481 cgcacgcgca cggccgcggc gcacggccgg ccgggacgac ctcggtggcg cggcagcccc
      541 ggcagcgcag cacgtccttg gcgccggacc acttgccgcg cttggcgatg ggccggccct
      601 cgatctccat gatgtcgagg gcgaagttca ggaccggcgc gttggcgatg gacgtgccca
      661 ccccgtaggc gtccgcgatc gggttgaggt gcaggatctc gtactcgtcg acgccgcccg
      721 aggcgatcat cttgacgtgc tcgaagcccc ggaggtcgag ctcccagcga acctcctgga
      781 tgatgcggaa gaagtcgccg cggcgtgagg agggcgtgtc gagccgcacc gcgtagaggt
      841 ccttgccgag ggcctcggcg acgcggaggg cctcgaactt ctcgtcctgg agcgtgtcga
      901 tgagcgccac gcgccgcacc ttggagtcga ccacctcgtg gaaggcgcgg agcgcttcga
      961 cggtgtcgcc catcatgagc acgagcgagt gggggatggt gcccatgggg tcggcgtcga
     1021 tcagctcggc cgacttcgtc accgccacgc cgtcgcagcc cccgatgaag gcgttgcgct
     1081 cgatcatcgg cgccagcgtg gggtgcatgc gccgggcgcc gaacgagatc acctgacgct
     1141 cgcccgcggc cttcttgcag cgcgccgcct tggtcgcgat gccggaggcc tggcagagca
     1201 ggcccagcag cgcggtctcg tacttcgccc actcgacgta ggtcccctcg acgaccagca
     1261 ccggctgata cggcgcgaag agcgtgccct cgtccatcgc gcgcgcgtcc accggaacac
     1321 cctcgaggag cgccgccacc tcctggatcc cggcgagcac gccccactgc cagtcctcgg
     1381 gcaggctctt gaggtagacc tcggccttga cccgcttgcg atcgcgccgg tgggtcagga
     1441 tctcgacggt gcgcgcgaag tagacgtccg aaacctcgcc gctcttgatc tcggcgtcgg
     1501 atgcggtgtg aaacgggcgc ttcggggtcg tcatcgcgtt gtccggccct ccggagcggc
     1561 accggggcgg cccgaaagtg gtggaaattc tggcagaagc ctaccggtaa gtcaatcaaa
     1621 cccctcccga accccacccc ggccggcgct tcctcagcga gtcaccgatt cgcgagacta
     1681 cgaggtcaga tgctcagcga cttcaagtac gcccggaact cgtcggcgag gtcggggcgg
     1741 gccagcgcga actccaccgt cgccttgagg aagccgagct tgtcgccggt gtcgtagcgc
     1801 ttgccctcga actccaccgc gtacatcgga cgctgcgccc gcagccggcg aagagcgtcc
     1861 gtgagctgga tctcgccgcg tcggtccggg cgcgtctcgg ccaggatgtc gaagaggtcg
     1921 ggcgtgagca cgtagcgccc gatgatggcc aggtcggagg gcgcctcctc ggcgcgcggc
     1981 ttctccacca gatcgcggat ctcgtagacg cgggcgccgt cggccccctg caccggacgc
     2041 gcgtcgatga caccgtactg gtggatacgc gcgcgcggca cgcgctcgac cgctatcacg
     2101 gggccgtcca gacgctcgta gacgtcgagg agttgccgca tgcagggcac cggcgcgccg
     2161 ccgatgatgt cgtcgccgag gaagacggcg aagggctggt cgcagacgag cggctgcgca
     2221 cacagaatcg cgtggccgag cccgagcggc tccttctggc ggacgtagca cagcgaggcc
     2281 agctccgaga tcgtcttgat ctgtgccagc tcgtccacct tgccccgatc gttcaggtaa
     2341 cactcgagct cgaaggcggc atcgaagtgg tcctcgatcg cgcgcttgtt gcggccggtc
     2401 acgatgatga tctcggaaat tcccgaggcc acggcctcct cgaccacgta ctgaatggtg
     2461 ggcttgtcga cgaggggcag catctccttg ggctgggcct tggtggcggg caggaaccgc
     2521 gtgccgaggc ccgcggccgg gaacaccgct ttgcgtacgg tcatgggcgt ggcctcctcc
     2581 acgttcatga taccctcggc cttctcacgc cgcccgtcgt ggccggcgcg cgtcgccgct
     2641 gcgccgcgtc cgccggacgg agagcacggc gagcgacacc gcgtacgcgc ccagcgccgc
     2701 gaccgctccg acgacggcgc agcccacgag gagcgccacc gacagctcgc ggaagagcgc
     2761 cagggcatcg tgccaggaga cgctccgcca gtgctcgagc aggtagtcga gccaggccgc
     2821 gcggaggcct tccgggtccg gcaccagcag gccgccaatc ttcagggagg cgccgtacac
     2881 gatcggggcg acccacggga ggttgagcca cgtcccggtg accgtggcgg ctcggttgag
     2941 gccgaagatc caggcgacgg cgatcgacag cacggtctgg agcccccaga acggcgtgca
     3001 gccgatgaag acgccgacgg cgagcgccca ggcgatcttc cggggggggt cgtccaggtg
     3061 cagcagcgcg tggagtcgcg cgccgagtcg cagtcggcgt cgggtcacgg ctcgccgcgc
     3121 ggcagccgga ccgcctgaaa cgcctcagcg tccttcacgg gccgggaagc cgcggaagtt
     3181 ggcgcgggac aaggccagtt cgaagatcgt cgagaaggcc agagcctcat catcgttcat
     3241 caatgataag tcaagcgaac gggtcgaggg tcgagagaag agggtcggcg tgcgcacggc
     3301 ggcggatcaa agagcatggc gtcgagcaag gcgggccagc accacgaact ggtaggcgaa
     3361 gaggcagggc atgctgcggg ccacgaaggc gctcagcgtg tggacggcgg ccagtcccct
     3421 cccgtgccac cgggacggca ccacctggta gagcggcacc ggggtcgccg tgaaccgctc
     3481 ggcggcgagc gctgccgtcg ccagcagcgc ctcgagcgtg cgtcgtgtga aaaatctcag
     3541 atgcgtgcgg tccaggaggc cgcggtcggc gtagtcgaac cttccggcga gcagaccgag
     3601 ccggacccac aggtgggcga cgttggggat ggagacgacg acggcgccgc cgggcgccag
     3661 gtgcgtcacg aggcgctcca gcgcgctctc ggggtcggcc aggtgctcca gcacgtccgc
     3721 gcagacgatc acgtcgaacg gcccgccgag gtcggggagc ggtctcccgg cgctcgtcac
     3781 gatcatccgc tcgcagtgac ccgcgcccgc ccgcgccgct tcggggtcga cctcgatcgc
     3841 cgtgacgcgc cagccctgct cggtcagcgg ccggctcagg agcccctcgg cggcgccgac
     3901 gtcgagcagc cgccgcccac ggccggcgcc aagccagtcg aggat
//
LOCUS       DATIAZ010000031         4714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1716591, whole genome shotgun sequence.
ACCESSION   DATIAZ010000031 DATIAZ010000000
VERSION     DATIAZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716591"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VLF19_01075"
     CDS             <1..473
                     /locus_tag="VLF19_01075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSE91869.1"
                     /translation="SFVVVPGTGILLNNGMIWFDPEPGKPNSVAPGKRALVNMVPVLG
                     FERGRPCFTLGAPGGRTIISAIPQVVANLADGATAQAAIEAPRLHTEGGDVLVSDRVG
                     ERAVSDLARRGHRVVAKRETYATLNFARPVAVRVTPQGLEAGLEQYAAAAAAGV"
     gene            897..1208
                     /locus_tag="VLF19_01080"
     CDS             897..1208
                     /locus_tag="VLF19_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91870.1"
                     /translation="MADVSLTAAFSTVPAGEQEDIRRVLVETVEQIARDDKTFGRAKL
                     VFTESDERCGLTAELDGVKREGVPLQIDIEQLEDAKSSAGARAELKEALRSYFRRVQG
                     K"
     gene            1205..1903
                     /locus_tag="VLF19_01085"
     CDS             1205..1903
                     /locus_tag="VLF19_01085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HSE91871.1"
                     /translation="MTDDGVLLFAWRGCLFRVTDEVQPPKAGSLFFCRSVRVRPGERV
                     LELGAGLGLFAVLASRAGARVVATDIVPEAVEAVRLNAHLNGVSVDVRLGDCYAPVTG
                     ERFDLICTNPPQMPTPPDRERDDAVAAADNGGADGWAILDRVIAGACDHLSPGGRLVF
                     SIFGFLGRKKALAKLEARGLAPSVIATEVQRFPRIGHERLDHIRRVDPEGSVPPGPAP
                     TKVERLIIQGELSH"
     gene            1900..2679
                     /locus_tag="VLF19_01090"
     CDS             1900..2679
                     /locus_tag="VLF19_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HSE91872.1"
                     /translation="MRRLIVNADDFGMTRGVSAGILRAHRHGIVTSTTVVVTGEIDHE
                     QLAAARDSALGLGLHVNLTLGKPLSHGRSLVDAQGRFVRDARRAAARADARDVEREVD
                     AQIERFVTLVGRPPTHLDSHHHVGLHEPVAGVVLEAARRLGVPVRSQNDAARRRARSA
                     GLGTPDAFFGESGPDAYWTPARTFRRLRDLPAGISEFMTHPGIFDETLGHSRYGRQRE
                     TELVGLGTLAARAAASALGITLCSFKALGTPMPVRRPSTSR"
     gene            2693..3676
                     /locus_tag="VLF19_01095"
     CDS             2693..3676
                     /locus_tag="VLF19_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSE91873.1"
                     /translation="MSGVLLGVDVGGTTTAAGLVTAEGEVLLEESAPTHGAGPATSWR
                     TIVTLIERVRRGADARGLAIHGIGVGIPGVVDAPAGRVGDEAPHVEDLRNSPVASRLT
                     DRFGLPTFVDNDVNALALAEHFFGLGRGVPSLVVLAPGTGFGSGIVLDGRLVRGAHGF
                     GGELGHVPVKFDGAPCFCGGRGCLAAYASGRGVVERARDRVAGHAGATLLRRAGGDPM
                     AITAPLVFEAAASGDVESGAVVDEACRALGAIIAVVVNGLNPDVVVVTGGVAAAYAPL
                     ERTVLRHAGEHAFARALAGTTVRVVPGDKRLTMRGAAALVRYETELKSRPR"
     gene            complement(3747..4370)
                     /locus_tag="VLF19_01100"
     CDS             complement(3747..4370)
                     /locus_tag="VLF19_01100"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSE91874.1"
                     /translation="MRGVIRLVPVLARLGLIVVFVLAVGTASPRLIVSSPPPAARATA
                     ALSGPAVGRITLVGAAEAAPAAPGFRVKVLDSSRYVDTRELIGKRVLVLRFQSSYCKP
                     CGRESAGLDRIAARYRQRDVEVLALHVQDTAVDTRRFLKRHRATYAVALDPTLTIGNR
                     YGFKGTPYTVVVDRRGEMVAQIHGVSAVSRLPRILDETLAADPPPKP"
     gene            4391..>4714
                     /locus_tag="VLF19_01105"
     CDS             4391..>4714
                     /locus_tag="VLF19_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSE91875.1"
                     /translation="MSDLEHELRKVVAGDVRFDTGSRLLYSTDASMYQVEPVGVVIPR
                     DRDDVEAAMTVARTHGVALLPRGGGTSLTGQTVNHALVLDFSRWMNQVLEVNETERWA
                     RVQPGL"
BASE COUNT          647 a         1699 c         1731 g          637 t
ORIGIN      
        1 gatccttcgt cgtcgtgccg gggacgggga tcctgctgaa caacggcatg atctggttcg
       61 atcccgagcc gggcaagccc aactcggtcg cgccgggcaa gcgcgccctc gtcaacatgg
      121 tgcccgtgct cggcttcgag cgtggccgtc cctgcttcac gctcggcgcc cccggcgggc
      181 ggaccatcat ctcggcgatc ccgcaggtgg tcgccaacct cgccgacggc gccacggcgc
      241 aggccgcgat cgaggctccg cggctgcaca ccgaaggtgg cgacgtgctc gtcagcgacc
      301 gcgtcggcga gcgggcggta agcgacctgg cccgccgcgg ccatcgtgtc gtggccaagc
      361 gcgagaccta cgcgacgctg aacttcgcgc ggcccgtggc ggtgcgcgtc acgccgcagg
      421 ggctcgaggc gggactcgag cagtacgcgg cggcggcggc cgccggagtc tgatcgcggc
      481 ttctgtggcc ggacgtcggg cggcgcagac tctgcccagc gccccgacgg cagacacgag
      541 ggagcgggtt ccgtgggccg accgtcgggg cggcggaggc actgcgtgcc gccccgacgg
      601 cgagacaaga gatccgacgc ccaggcgtga gccgcgagga gagcacacga tggcgcagct
      661 cgaagagaaa gtgatcgcgt cgtggaaata caagaagggc gcgctcgacc ccaagaccgc
      721 cgcgctgtgc tacctggccg cgaacctcgc cgccggcaac acgcactgag cgaagcgcga
      781 cctggcgggc gccaggacgg ccggggcgac cgaggacgag gtgcgcgagg ccatcagctt
      841 cgcgatccgc gccaacgcgg cgaagaccca tgccgacatc ctccaggtgt ggaccgatgg
      901 cggacgttag cctgaccgcg gccttctcga cggtcccggc cggtgagcag gaagacatca
      961 ggcgcgtgct cgtcgagacc gtcgagcaga ttgcccgcga cgacaagacg ttcggccggg
     1021 ccaagctcgt cttcaccgag tccgacgagc gttgcgggct gaccgccgag ctggacggcg
     1081 tcaagcgaga aggagtgccg ctgcagatcg atatcgagca gctcgaggac gccaagtcct
     1141 cggcgggcgc gcgcgccgag ctcaaggagg cgctgcgctc gtacttccgg agggtgcagg
     1201 gcaagtgacg gacgacggcg tcctgctctt cgcctggcgg ggctgcctct tccgcgtgac
     1261 cgacgaggtc cagccgccca aggccggctc cctcttcttc tgccgcagtg tcagggtgcg
     1321 gccgggcgag cgcgtcctcg agctcggcgc cggcctcggg ctcttcgcgg tcctggcctc
     1381 gcgcgcgggc gcgcgggtgg tcgccaccga catcgtcccc gaggccgtgg aggccgtccg
     1441 cctcaacgcc cacctcaacg gcgtgagcgt ggacgtccgg ctcggggact gctacgcgcc
     1501 cgtcaccggc gagcgcttcg acctgatctg cacgaatcca ccgcagatgc cgaccccgcc
     1561 cgaccgcgag cgtgacgacg cggtcgctgc ggccgacaac ggcggcgccg atggctgggc
     1621 catcctggac cgggtcatcg ccggcgcctg cgaccacctg tcgccgggcg gccggctggt
     1681 cttctcgatc ttcggcttcc tcgggcggaa gaaggccctc gccaagctgg aggcccgggg
     1741 gctcgcgccg tccgtgattg cgaccgaggt ccagcgcttc ccgcgcatcg gccacgagcg
     1801 gctcgaccac atccgacgcg tcgatcccga gggctcggtg ccaccgggtc cggctcctac
     1861 caaggtcgag cgcctcatca tccagggaga gctaagtcat tgagacggct gatcgtgaac
     1921 gccgacgact tcggcatgac tcgtggggtg agtgccggca tcctccgcgc tcaccggcat
     1981 ggcatcgtga cgagcacgac ggtggtggtg acgggcgaga tcgatcacga gcagctcgcc
     2041 gccgcgcgcg acagcgccct gggtcttggc ctgcacgtga atctcacgct aggcaagccg
     2101 ctctcgcacg gccgctcgct ggtcgatgcc cagggccgct tcgtgcgcga cgcgcgccgt
     2161 gccgcggcgc gcgcggacgc ccgtgacgtc gagcgcgagg tcgacgcgca gatcgagcga
     2221 ttcgtcaccc tggtcgggcg ccctccgacc cacctcgaca gccaccacca cgtcggcctg
     2281 cacgagcccg tggcgggcgt cgtcctcgag gctgcgcgca ggctcggcgt gcccgtgcgg
     2341 agccagaacg acgccgcgcg ccggcgggcg cggtcggccg ggctcgggac acccgatgcc
     2401 ttcttcggcg agtcggggcc cgatgcctac tggacgcccg cccgcacctt caggcggctc
     2461 cgcgacttgc cggcggggat ctccgagttc atgacgcatc ccggcatctt cgacgagacg
     2521 ctcgggcaca gccgctacgg gcggcagcgc gagacggagc tggtcgggct cggcacgctg
     2581 gccgcgcggg cggcggcgtc ggccctcggg atcacgctct gcagcttcaa ggcgctcggc
     2641 acgcccatgc ctgtccgtcg cccctcgacg tcgcgatagt cggctgtccg ccgtgagcgg
     2701 ggtgctgctc ggtgtcgacg tcggggggac gacgaccgca gccggcctcg tcaccgcgga
     2761 aggcgaggtc ctgctcgagg agtccgcacc gacccacggg gcggggccgg cgacctcgtg
     2821 gcgcacgatc gtcacgctca tcgagcgagt ccgcaggggc gcggatgccc gcggcttggc
     2881 catccacggc atcggcgtcg gtatccccgg cgtggtggac gcgccggcgg gacgtgtcgg
     2941 cgacgaggcg ccccacgtgg aggacctccg taactcgccg gtcgcatcgc ggctgaccga
     3001 ccgcttcggg ctgccgacgt tcgtcgacaa cgacgtcaac gcgctcgcgc tggccgagca
     3061 cttcttcggg ctcggccgcg gtgtgccctc gctggtcgtc ctggctcccg gcacgggctt
     3121 cggctcgggc atcgtgctcg acggccggct cgtgcgcggc gctcacggct tcggcggcga
     3181 actcggccac gtgccggtga agttcgacgg cgcgccgtgc ttctgcgggg ggcgaggctg
     3241 cctggccgcc tacgccagcg gccgcggcgt cgtggagcgc gcgcgagacc gggtggccgg
     3301 ccacgccgga gcgacgctcc tgcgccgggc gggaggcgat ccgatggcga tcacggcgcc
     3361 gctggtcttc gaggccgcgg cctccgggga cgtcgagtcc ggcgccgtcg tggacgaggc
     3421 ctgccgagcg ctcggcgcca tcatcgccgt cgtcgtcaac ggcctcaacc cggacgtcgt
     3481 ggtggtcacg ggcggcgtcg ccgccgcgta cgcgccgctc gagaggaccg tgctgcggca
     3541 cgccggagag cacgcgttcg cccgcgcgct cgcggggacg accgtccgcg tcgttcccgg
     3601 cgacaagcgg ctgacgatgc gcggcgccgc cgcgctcgtg cgctacgaga ccgaactcaa
     3661 atcccgtccg cggtagtctc ccgagccacg ccgtcgtctc cctttcggag gctcgcgatc
     3721 ccgcccgccg gccgcgcgag gatccctcag ggcttcggcg gcggatcggc ggcgagcgtc
     3781 tcgtcgagga tcctgggcag ccgtgacacc gcggacacgc cgtggatctg cgccaccatc
     3841 tcgcccctgc ggtccacgac gaccgtgtac ggcgtgccct tgaacccgta ccggttgccg
     3901 atggtcaagg tcggatcgag cgccaccgcg taggtggcgc ggtgacgctt gaggaagcgc
     3961 cgcgtgtcga ccgccgtgtc ctgcacgtgc agcgcgagga cctcgacgtc gcgctggcgg
     4021 tagcgggcgg cgatgcggtc gaggccggcc gactcgcggc cgcacggctt gcagtacgac
     4081 gactggaagc gcagcacgag gacacgcttg ccgatgagct cgcgcgtgtc cacgtagcgc
     4141 gagctgtcga ggaccttcac gcggaagccc ggcgccgccg gcgccgcctc ggcggcgccg
     4201 accagcgtga tccgcccgac cgccggaccg ctaagcgcgg ccgtcgcacg cgccgccgga
     4261 ggcgggcttg acacaatcag gcgcggcgag gcggtgccga cggcgagtac gaagaccacg
     4321 atgagcccga gccgcgcgag cacggggacg agccggatca cgccgcgcac gcgcgttata
     4381 atacctcccg gtgagcgacc tcgagcacga actccgcaag gtcgtcgctg gcgacgtccg
     4441 cttcgacacc gggtcccggc tgctgtactc gacggatgcc tcgatgtacc aggtggagcc
     4501 cgtgggcgtc gtgatcccgc gcgaccgcga cgacgtcgag gcggccatga ccgtggcccg
     4561 cacgcacggc gtggcgctgc tcccccgggg cggcggtacc tcgctcaccg gccagacggt
     4621 caatcacgcg ctggtcctcg acttctcgcg ctggatgaac caggtgctgg aggtgaacga
     4681 gacggagcgg tgggcgcgcg tgcagccggg gctc
//
LOCUS       DATIAZ010000032         5361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1585420, whole genome shotgun sequence.
ACCESSION   DATIAZ010000032 DATIAZ010000000
VERSION     DATIAZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585420"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="VLF19_01110"
     CDS             complement(<1..1744)
                     /locus_tag="VLF19_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSE91876.1"
                     /translation="MTIDPITLAVVWGALSSIAVEIGTTVHKTAYSEQAREGQDFSVA
                     IFDRAGRMVAQGPYSPGHMGAMSFAVKNALAAHPVATLRPGDAILLNDPLLGSGHFPD
                     FFITQPAFHRGELIGFVVNIVHHTDVGGQRPGSQGVVGILDYFQEGLRIPPTKVWREY
                     REDEGVIGIVAANTRTPDRVLGDLRAQRSALRVGEQRLVEVVERYGRDTVFTVMDEII
                     ARTETAMRAAIRAVPDGVYTFEDFLDDYGPDTEPVRVRCAVTVSGDALTVDYEGSSPQ
                     TPSGLNSYINYTRSYSYAAIKCLTDPLGPMNEGALAPITVRAPEGSFLNPRPPAGGGP
                     RALICYRVFEAVLGALAPALPERVAAAASHMANPTFGGRDRRHGRRFVAYELVLSGTG
                     ARATRDGCEGMSWAFNASNIPVEAQEARHPIVVERFELQRDSGGPGRYRGGCGVRRDM
                     RFLADDGLLTNLTDRQRLAPYGLFGGGPGRLGRTLINPEAGEVVVHGKESRGFAYGDV
                     ISFQQAGAGGYGDPLERDPARVLEDVLDDYVSIEAARADYGVVLAGAGADLYVDALAT
                     RTLRAELRTRARASP"
     gene            complement(1741..3807)
                     /locus_tag="VLF19_01115"
     CDS             complement(1741..3807)
                     /locus_tag="VLF19_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSE91877.1"
                     /translation="MPYRIGFDVGGTFTDFVLQSPTGELTTAKRLTTYPDPSVACLTG
                     LDELTAAAGVAWPDVVQAVHGTTLGSNVVIERKARGVGLLTTRGFRDVLIIGREKRYQ
                     VYDLQIEKPPPLIPRRLIGEVTERVLADGSVRTPLDEADARRAIRALAARGVTTLAVC
                     FLHAYLNPAHERRIALLAAEEAPHVAVTLSHEVSPTFREYERTSTTVVNAYVMTAVRA
                     YLRDLRSALGERGYRGRLFVMQSSGGVATAEAMERYPVRMIESGPAAGALMAAAYGEL
                     AGQRDLIAFDMGGTTAKLALIEQGRPLTTTAFELHRVDNVPGSGLPMNIQAVDLVEIG
                     AGGGSIARARMGVIAVGPESASSTPGPACYGRGGLEPTVTDANLVLGYLNPDDFAGGA
                     MRLSLGAAARAIEERLARPLGLALEEAAWGVHQVVTTNMELATRVVSIERGRDPRDLT
                     LVAFGGSGPVHGCRLAQALGVPRVILPAAAGVTAAIGLLAAELRLDVARTWAGRLDAV
                     DPGILTAMYEEMARQAVDVVRESGVTGEIAITRAADARYAGQGYELTVSVPPGALSAT
                     VLSRVRAGFDEAYAARYGYASPGEPVEVVTWKLAAVGGAPRVALAKAPAETGELPRKA
                     VRRAWFPEARGYVDTPVYHRYRLWAGFALTGPAIVEERESTTVLPPGATAVVDEHASL
                     IVTVRP"
     gene            complement(3814..4539)
                     /locus_tag="VLF19_01120"
     CDS             complement(3814..4539)
                     /locus_tag="VLF19_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSE91878.1"
                     /translation="MNYRPGIPRYLQIADALRRALKGEGERIPSEHQLCALYKVSRPT
                     VRQALDVLVQEGLLYRHAGRGTFSTPTPGGDRKLRVIGSVDDMIALGEETWFKLLTRE
                     VVRVAANIAQALRLPPGSEAYRIVGVRHGDTGPFQHVTAYLPRHMAALLSEEDLSKTS
                     LIAVIERHLGRTIKVLEQVVDAALAPRHVAELLQVRPRTPLLLFERTYFTAGGEPVEH
                     AVTYQSSRRYPYRVVLSRAERRS"
     gene            complement(4871..>5361)
                     /locus_tag="VLF19_01125"
     CDS             complement(4871..>5361)
                     /locus_tag="VLF19_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSE91879.1"
                     /translation="MAAATKRPAQVLGLHFFNPVPLMRLVEVVRTILTDESAVAAATE
                     WIHAIGKVPVQTKDSTAFIVNRLLVPYLLDAIRLYEGGLASLEDIDQAMKLGCGYPMG
                     PFTLLDLVGLDTTMYVAEVMFDEYREPRYAPPPLLKRMVLAGHLGRKTGRGFYTYDEP
                     RR"
BASE COUNT          777 a         1907 c         1956 g          721 t
ORIGIN      
        1 cgggcgacgc ccgcgctcga gtgcgcagct ccgcccgcag cgtccgggtc gcgagcgcgt
       61 cgacgtacag gtcggcgccg gcgccggcga ggacgacgcc gtagtcggcg cgcgccgcct
      121 cgatcgacac gtagtcgtcc agcacgtcct ccaggacgcg cgcgggatcg cgctccagcg
      181 ggtcaccgta gccgccggcg ccggcctgct ggaacgagat cacgtcgccg taggcgaagc
      241 cgcgcgactc cttgccgtga acgaccacct caccggcctc agggttgatc agcgtccggc
      301 cgagccgtcc cggccccccg ccgaagagcc cgtagggcgc gagccgttgc cggtccgtga
      361 gattggtgag caggccgtcg tcggcgagga agcgcatgtc gcgtcggacg ccgcaaccgc
      421 cccggtacct cccgggcccg ccggagtcac gctgcagctc gaaccgctcg acgacgatcg
      481 gatggcgcgc ctcctgcgcc tccacgggga tgttcgaggc gttgaaggcc cacgacatgc
      541 cctcgcagcc gtccctggtc gcgcgggcgc cggtgcccga cagcaccagc tcgtaggcga
      601 cgaagcggcg tccgtgccga cgatcgcggc cgccgaaggt cgggttcgcc atgtgcgagg
      661 ccgccgcggc cacgcgctcc ggcagcgccg gcgcgagcgc gccgagcacg gcctcgaaga
      721 cccggtagca gatcaacgcc cgtggaccgc cgcccgccgg cggccgggga ttcaggaacg
      781 agccctcggg cgcccgcacg gtgatcggcg ccagcgcgcc ttcgttcatc gggccgaggg
      841 ggtccgtgag gcacttgatc gcggcgtacg agtacgagcg cgtgtagttg atgtacgagt
      901 tcagtcccga cggcgtctgc ggggacgagc cctcgtagtc caccgtcagc gcgtcgccac
      961 tgaccgtcac ggcgcaccgc acgcgcacgg gctcggtgtc cgggccgtag tcgtccagga
     1021 agtcctcgaa cgtgtacacg ccgtcgggca cggcgcggat cgccgcgcgc atggcggtct
     1081 cggtgcgggc gatgatctcg tccatgaccg tgaacaccgt gtcccgcccg tagcgctcga
     1141 cgacctcgac gagtctctgc tcacccacgc gcagcgcgct ccgctgggcg cgcaggtcac
     1201 ccagcacccg gtcgggcgtg cgcgtgttgg cggcgacgat gccgatcacg ccctcgtcct
     1261 cgcggtactc gcgccagacc ttggtcggcg ggatccggag tccctcctgg aagtagtcga
     1321 ggatgccgac cacgccctgg ctgcccgggc gctggccgcc gacgtccgtg tggtggacga
     1381 tgttcacgac gaagccgatc agctcgcccc ggtggaacgc cggctgcgtg atgaagaagt
     1441 cgggaaagtg ccccgacccg agcagagggt cgttcagcag gatcgcgtcg cccggacgca
     1501 gcgtcgccac cgggtgcgcg gccagggcgt tcttgaccgc gaaggacatc gcgcccatgt
     1561 ggccgggcga gtacggcccc tgggccacca tccgaccggc tcggtcgaag atggcgaccg
     1621 agaaatcctg gccttcgcgc gcctgctccg agtacgccgt cttgtgcacc gtggtgccga
     1681 tctcgacggc gatcgacgag agcgcgcccc agacgacggc gagcgtgatc gggtcgatcg
     1741 tcacggcctc accgtcacga tgaggctcgc gtgctcgtcc acgacagcgg tcgcacccgg
     1801 cggcagcacc gtggtcgact cccgctcctc cacgatcgcg gggccggtca gcgcgaaacc
     1861 cgcccagagg cgatagcggt ggtacacggg tgtgtccacg tagccgcgcg cctcgggaaa
     1921 ccaggcgcgc cggacggcct tgcgcgggag ctcgccggtc tccgcggggg ccttggccag
     1981 cgcgacccgc ggcgcgccgc cgacggccgc gagcttccag gtgacgacct ccaccggctc
     2041 tccgggactc gcgtagccgt agcgcgcggc gtaggcctcg tcgaagccgg cgcgcacccg
     2101 tgagagcacg gtggcgctca gcgcacccgg cggcaccgac acggtcagct cgtagccctg
     2161 gccggcgtag cgagcgtccg cggcacgggt gatcgcgatc tccccggtga caccggattc
     2221 gcggacgacg tccacggcct gccgtgccat ctcctcatac atcgccgtga ggatgccggg
     2281 gtcgaccgcg tccagtcggc ccgcccaggt gcgcgcgacg tctaagcgca gctcggcggc
     2341 gaggagcccg atggccgcgg tcacgccggc ggcggcgggc aggatcacgc gcgggacgcc
     2401 gagcgcctgc gcgagccggc agccgtgcac cggccccgag ccgccgaagg ccacgagggt
     2461 gaggtcgcga ggatcgcggc cgcgctcgat cgacaccacg cgcgtggcga gctccatgtt
     2521 cgtggtgacg acctggtgga caccccacgc cgcctcctcg agcgcgagcc cgagcggccg
     2581 agccagccgc tcctcgatcg cgcgcgccgc tgcgccgagc gacagtctca tcgcgccccc
     2641 ggcgaagtcg tcggggttca ggtagccgag gacgaggttg gcgtcggtca ccgtcggctc
     2701 gaggccaccg cgaccgtagc aggcggggcc gggcgtggac gaggcgctct ccgggccgac
     2761 ggcgatcacg cccatccggg cgcgcgcgat ggagccgccg ccggcgccga tctcgaccag
     2821 gtccacggcc tggatgttca tcggcaggcc gctgccggga acgttgtcga cccggtgcag
     2881 ctcgaaggcc gtcgtggtca ggggccgacc ctgctcgatc agggcgagct tggcggtggt
     2941 gccgcccatg tcgaaggcga tgaggtcgcg ctggcccgcg agctccccgt aggccgcggc
     3001 catgagcgcg cccgcggccg ggccggactc gatcatgcgc acggggtagc gctccatcgc
     3061 ctccgcagtc gcgacgccgc cggaggactg catgacgaag agtcgcccgc gatacccgcg
     3121 ctcgccgagc gccgagcgga ggtcccggag gtacgcgcgt accgcggtca tcacgtaggc
     3181 gttgacgacc gtggtcgagg tgcgctcgta ctcccggaac gtcggcgaca cctcgtgcga
     3241 cagggtcacc gccacatggg gcgcctcctc ggcggcgagg agggcgatgc gccgctcgtg
     3301 ggcaggattg aggtaggcgt gcaggaagca cacggcgagc gtggtgacgc cgcgggcggc
     3361 cagcgcgcgg atcgcccgcc gcgcgtcggc ctcgtcgagc ggcgtgcgca ccgagccgtc
     3421 ggcgagcacc cgctcggtca cctcgccgat gagccggcgg ggaatcagcg ggggcggctt
     3481 ctcgatctgg agatcgtaga cctggtagcg cttctcccgg ccgatgatca gcacgtcgcg
     3541 gaacccgcgc gtggtgagga gccccacgcc gcgcgccttg cgctcgatca cgacgttgga
     3601 gccgagggtg gtcccgtgga cggcctggac gacatcgggc caggcgacgc cggcggccgc
     3661 cgtcagctcg tcgaggcccg tcaggcaggc caccgacggg tcgggatacg tggtcagccg
     3721 cttggcggtc gtcagctcgc ccgtgggcga ctggagcacg aagtcggtga acgtgccgcc
     3781 gacgtcgaac ccgatgcggt acggcaccgg ggatcagctc ctgcgttcgg cccgggacag
     3841 cacgacgcgg tacgggtagc gccggctcga ctggtaggtc accgcgtgct cgaccggctc
     3901 gccgcccgcc gtgaagtacg ttcgctcgaa gaggagcagc ggcgtgcgcg gacggacttg
     3961 caacagctcg gccacgtggc ggggtgcgag tgcagcgtcg accacctgct cgagcacctt
     4021 gatcgtccgt ccgaggtggc gttcaatcac cgcgatcagc gacgtcttcg acaggtcctc
     4081 ctcggagagc agcgcggcca tgtggcgcgg caggtacgcc gtcacgtgct ggaacgggcc
     4141 ggtgtcgccg tggcgcacgc cgacgatgcg gtaggcctcc gagcccggcg gcaagcgcag
     4201 cgcctgggcg atgttcgcag cgacccggac gacctcgcgc gtgaggagct tgaaccacgt
     4261 ctcctcgccc agcgcgatca tgtcgtccac cgagccgatc acacggagct tgcggtcgcc
     4321 gcccggcgtc ggcgtgctga aggtgccgcg gccggcgtgg cggtagagca acccctcctg
     4381 gacgaggacg tcgagggcct gccggaccgt cggccggctc accttgtaga gcgcgcacag
     4441 ctgatgttct gagggaatgc gctcgccctc gcccttgagc gcgcggcgga gggcgtcggc
     4501 gatctgaagg tagcgcggaa tgccgggacg atagttcacc gcggcgagcc tcctgctgtc
     4561 aggatgtcta gacatctaca ccactcgcgc gcggtcgtca acgcgcggcg catcccgtca
     4621 gaactggaag cggactccgt ggaggacgtc gtcgccgccg acttccccgg cgcgcccgcc
     4681 ctaaccgggt cgggccagcg cgtccgtgcc cccgtgcgtc acgaggtatt cgccgaagag
     4741 gctgctgcgg gcacccaccc tgaaagcacc gctccgggct cgccctgagg gacgaggagc
     4801 gtgccggcgc ccctcggctc caggggggaa gagggtgggc cccatcttga gaagggacct
     4861 cgaccgcggg tcagcggcgc ggctcgtcgt acgtgtagaa cccgcgaccg gtcttgcggc
     4921 cgagatggcc ggccagcacc atgcgcttga ggagcggcgg cggcgcgtac cggggctccc
     4981 gatactcgtc gaacatcacc tcggcgacgt acatcgtcgt gtccaggccc acaaggtcca
     5041 gcagcgtgaa cgggcccatc ggatagccgc agccgagctt catcgcctgg tcgatgtcct
     5101 cgagggaggc cagcccgcct tcgtagaggc gaatggcgtc gagcagatag ggcacgagca
     5161 ggcggttgac gatgaacgcc gtggaatcct tcgtctgcac cggcaccttg ccgatcgcgt
     5221 ggatccactc ggtcgccgcc gccacggccg actcgtcggt gaggatcgtg cgcacgacct
     5281 cgacgagcct catgagcggc acggggttga agaaatgcag cccgaggacc tgggcgggcc
     5341 gcttcgtcgc cgccgccatc g
//
LOCUS       DATIAZ010000033         8320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1059520, whole genome shotgun sequence.
ACCESSION   DATIAZ010000033 DATIAZ010000000
VERSION     DATIAZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059520"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VLF19_01130"
     CDS             <1..190
                     /locus_tag="VLF19_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HSE91880.1"
                     /translation="LRGAGDAEATAVTGQAFGRDPAFYGFMRRLETYERVFTPETTIV
                     LRPDSDLLRYLDSPRERR"
     gene            310..591
                     /locus_tag="VLF19_01135"
     CDS             310..591
                     /locus_tag="VLF19_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSE91881.1"
                     /translation="MHRERRCAGRARHHTALGAATGAVTAPEGDQRWLGMRGTTVSPL
                     RPAGIADFGGERIDVVSRGEYIEAGQPIEVIRVEGNRIVVRPERGQGEE"
     gene            595..1319
                     /locus_tag="VLF19_01140"
                     /pseudo
     CDS             595..1319
                     /locus_tag="VLF19_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
     gene            1332..1670
                     /locus_tag="VLF19_01145"
     CDS             1332..1670
                     /locus_tag="VLF19_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91882.1"
                     /translation="MEQILLLALFVVGALASQLARWLKARRPAEAEPAEGVEALPPRA
                     RPRQVTPVPVETSPLPDVRLLAPAPPSPPRRPPRSRLGRPSDLRGAIVLMAVLGPCRA
                     LEHEEPPLPR"
     gene            complement(1686..2453)
                     /locus_tag="VLF19_01150"
     CDS             complement(1686..2453)
                     /locus_tag="VLF19_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE91883.1"
                     /translation="MGKTTFDFSGQVVAVTGGARGIGRGSAEAFARAGARVYILDLDE
                     TGGEAVAAGIRKHGGHATFVVCDVTDWARVKAAFDRIVAEGEHLDVLVNSAGGFWKQL
                     SVEETPEDEWDKVIDLNLKSIFLCARAAIPAFKRQGRGRIVNIGSMAGVSALQPSSPP
                     YAAAKAGVHSLTRVLAFELGKHGVTANAIAPGTTATERVVAVRSKEQRDAIGRSTLVG
                     RIAEVSDMVGWVLFLASPEAGYFTGQTLSVNGGRLMV"
     gene            complement(2494..3450)
                     /locus_tag="VLF19_01155"
     CDS             complement(2494..3450)
                     /locus_tag="VLF19_01155"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HSE91884.1"
                     /translation="MATLTGQPTITTIHPHLGALVEGVDLARPLDDATFGAVFDAFQE
                     HSVLVFRDQRLTDEQQMAFSEHFGPLETTIRSISKGGRLHPNLVDLSNLDPEAEDRLM
                     DWGDRRMVYQSGNQLWHSDSSFKPVPAMASMLSGREVPPAGGETEFASLRHAWATLPD
                     ATRRLIEGRVAVHSIVYSRYTIAPGLFDPGQEQGVPPVRQALVRVNPVNGRKSIFVGS
                     HAWYVDGMDYAESRRLLDDLLAHATRPECVYQHRWRPWDLVMWDNRCVLHRGRPWDAA
                     RHRRVMRRTTVAGDGPTAEPPLATRTPAWAGIIPESVQGPTG"
     gene            3639..4853
                     /locus_tag="VLF19_01160"
     CDS             3639..4853
                     /locus_tag="VLF19_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE91885.1"
                     /translation="MSALSRRTLLKMGAGAAVLGAPGVLRAQGKDVVMLGLWPFTGAF
                     ADVGPILDRGMKMALEERGMKAAGRPIKYVTRDSETKAGSATRRAEEAVDGEGAKYII
                     GPWSSGVALAVSEVAKRKKVVHYFSGGTEDISGSRCHRYSFQWAASPYTAAKTVVDNF
                     MKANPKAKRWHLLVADYAFGWSVEKYIKEVGKDHGLEFVGADRHPLGEREFSNYVTKA
                     AGNKPDAVAMINFGLDAVTAAREIFNFGLTPKVPLIMTWSSGVEELIQLAPEIRENTW
                     VGSNFYYTADTPVARAFVKSYQEKHKNPPGYAPAAAYGMTRMVLAAMEKARSAEVPEV
                     IAALEGLEVDDLVGRMRVEPKTHQTLRPYFFMRCKKKDAMKHPMDFADIIATGSTPLP
                     AQYSACKDIGAV"
     gene            5051..5914
                     /locus_tag="VLF19_01165"
     CDS             5051..5914
                     /locus_tag="VLF19_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE91886.1"
                     /translation="MNAGLLFGQTLNGVATGMLYALMGIGLSIVTGVLNIPNFAHGAM
                     FALGAYLLFAAIQVVGNFWLALVLAPVGVLALGLVVEYAGIRPLYRAGHDYQLLLTFG
                     LSLIVTEGIIVVWGPIGMSQLPPDILRGGVDLGVTIYPKYRLFVMGMAGLLVAGSWLF
                     LEKTRYGAIMRAGIEDREMVALLGIDIHRLFTAAFGLGAYLAGIAGALTAPIRGLNPF
                     MGVDMLGIAFVVVALGGLGNLPGAIVAGIIVGVAQSLVSLVWPEASVAVIFAVMAAVL
                     LVRPQGLFGIR"
     gene            5976..6908
                     /locus_tag="VLF19_01170"
     CDS             5976..6908
                     /locus_tag="VLF19_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE91887.1"
                     /translation="MIRAWLAVLAAVVLLPLVVRHAIATEIWIFAIFGLGLNLLLGYT
                     GLLSFGQATFFGSAAYVGGYLLKHHGVPVLAVLAIGLAVGAVSAALVGYLCVQRSGLY
                     FIMLTFALNQMFFFTAYQWTSVTGGEDGMPGVPRPPLLGLDLNGTLTYYAFVTALFLM
                     SLWVMQRIVASPLGVILQAIRENEVRAEAVGFDARRFKLLAFVIGGAFSGVAGVLYAM
                     LFGIVPLEAIGFVFSGNVVFATLIGGSGSLYGPIIGSFIFIWLSESVSVIWQRWPLLL
                     GVAFVIVVLFFRGGVVEAWDRLRLWRTRRGAAED"
     gene            6889..7638
                     /locus_tag="VLF19_01175"
     CDS             6889..7638
                     /locus_tag="VLF19_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE91888.1"
                     /translation="MALLRTEGLTKTFGALTAVSDVSLAVEAGTLHSIIGPNGAGKTT
                     LFNLLTGQHAPTAGRIVLDGRDIAGTPPQRIAHLGVARSFQRTNVFPALTVLDNVWVA
                     AFARGSWRGLAWRPAARYADRRERALTALREVGLADKAGHRAREISHGEQRQLELAIA
                     LAATPRLLLLDEPAAGLSPDETRKMVQLVRALKGRYTIVLVEHKMDVIMTISDRISVM
                     HFGRLIAEGTPAEIREHPEVRRAYLGGMAAS"
     gene            7635..>8320
                     /locus_tag="VLF19_01180"
     CDS             7635..>8320
                     /locus_tag="VLF19_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE91889.1"
                     /translation="MILDVRRIDAHYGLAHVLHGLSLRVEEGEVVALLGRNGAGKTTT
                     MRAITGLTPPRAGDILYRGSSIAGRAAHHIARLGIALVPETRGIFSYLTARENLDIAR
                     RPGSRWSREIVLERFPKLREVLDRKGRFLSGGEQQMLAIARALMTGPDLLLLDEPSQG
                     LAPLVVETVMQTIAELKRERVSMLLVEQNAELALRLADRVYVIDHGTIVFEGTPASLR
                     ADVRVTSTYLG"
BASE COUNT         1134 a         2963 c         2939 g         1284 t
ORIGIN      
        1 cctgcggggc gccggcgacg cggaagccac cgccgtgacc ggccaggcct tcgggcgcga
       61 tccggccttc tacggcttca tgcggcgcct ggagacgtac gagcgcgtct tcacgccgga
      121 gacgacgatc gtgctccggc cggactcgga cctgttgcgc tatctcgact cgccgcggga
      181 gcgtcggtaa ccgcgcggtg cgggcgggag gccgggcagc tgcgggcgac ggcgccgtaa
      241 ggcgctgggg acgggcgtcg ctcgtggtgc tctgcctgct ggggtcggta cctgccgccg
      301 gcccgggcgg tgcacaggga gcgccgctgt gccgggcgcg ctcggcatca caccgccctc
      361 ggcgcggcca caggcgcggt cacggcgccc gagggcgatc agcgctggct gggcatgcgg
      421 gggacgacgg tgtcgccgct gcgcccggcc ggcatcgccg atttcggcgg ggagcgcatc
      481 gacgtggtct cgcgggggga gtacatcgag gcagggcagc ccatcgaggt catccgcgtc
      541 gagggcaacc gtatcgtcgt ccgccccgag cgcggacagg gggaggagtg agcgatggtg
      601 gggatcgaca tcggactcgt cggagtcctc gtgctcgtgg tggtcgcgct gatcctcgtg
      661 ctgtacctgg tcccgatccc cctctggatc gccgcgtggg cctccggcgc cgacgtcggt
      721 ctcttcacgc tgatcgggat gcggctccgg cgggttccgc cggcacggtc gtcaccgcgc
      781 ggatcagcgc cgtgaaggcg gggctcgaca tctcgatcaa cgacctcgag gcccactacc
      841 tggctggcgg caacgtcgtg cgcgtggtca acgcgctgat ctccgccgat aaggcgaaca
      901 tcgtcatgcc gttcaagcgg gcggcggcca tcgacctcgc cgggcgcgac gtcctcgagg
      961 cggtgaagat gtcggtcatc cccaaggtca tcgagacgcc acggatcgcg gccgtcgcca
     1021 aggacggcat ccagctcatc gccgtgtcgc gcgtgacggt tccggacgaa cctcgaccgg
     1081 ctggtgggcg gggcaggcga ggagaccgtc atcgcgcgcg tgggcgaggg gatggtcaga
     1141 tgcgggcgcg ggtcgtggag gccgaggccg aggtgccgcg cgcgatggcg gaggccttcc
     1201 ggaacgggaa tctggggatc atggactact accggatgaa gaacgtgcag gccgacacgt
     1261 cgatgcgtga gtcgatcgcc gggccaggcg gcgaggcccc gccgccggtc acgcgctagg
     1321 aaggggtgcg cgtggagcag atcctcctcc tcgccctgtt cgtggtgggg gcgctggcga
     1381 gtcaactcgc gcgctggctc aaggcgcgcc ggccggccga ggcggaaccg gccgagggcg
     1441 tcgaggccct cccgccgcgt gcgcgcccgc gtcaggtgac gccggtaccc gtggagacgt
     1501 ccccgctccc ggacgtccgg ctgctcgcgc cggcgccgcc gtccccacca cggcgacccc
     1561 cacgctcgcg cctcgggcgg ccgtccgacc tgcggggcgc catcgtgctg atggccgtgc
     1621 tcggcccgtg ccgggcgctc gagcacgagg agccgccgct cccgcggtag cgcctggctg
     1681 gctcctcaca ccatcaggcg cccgccgttg acggacaggg tctgccccgt gaagtagccg
     1741 gcctccggcg aggccaggaa gagcacccag cccaccatgt ccgacacctc tgcgatgcgg
     1801 ccgacgaggg tggacctgcc gatcgcgtcg cgctgctcct tgctgcgcac ggcgacgacg
     1861 cgctcggtgg cagtcgtacc aggcgcgatg gcattggcgg tcacgccgtg cttgcccagc
     1921 tcgaaagcga gcacgcgggt gagcgagtgc acgccggcct tggcggcagc gtagggcggg
     1981 gaggagggct ggagcgcgct cacgccggcc atcgagccga tgttgacgat gcgtccgcgg
     2041 ccctggcgct tgaaggccgg gatggccgcc ctggcacaga gaaagatcga cttcagattc
     2101 aggtcgatga ccttgtccca ctcgtcttcg ggcgtctctt ccaccgagag ctgcttccag
     2161 aagccgcccg cgctgttgac gagcacgtcg aggtgttcgc cctccgcgac gatgcggtcg
     2221 aacgccgcct tcacccgcgc ccagtcggtc acgtcgcaga cgacgaaggt cgcatggccc
     2281 ccgtgcttgc ggatgccggc ggcgacggcc tcgccgccgg tctcgtccag gtccaggatg
     2341 tagacgcgcg cgcccgcccg ggcgaaggcc tcggcggagc ccctgccgat gccccgcgcg
     2401 ccgccggtca ccgccaccac ctgtcccgag aagtcgaacg tcgtcttgcc catgccggga
     2461 agatattgcc ggatcgcccc gtgggcggag cgatcagccc gtgggacctt gcacgctctc
     2521 cgggatgatc cccgcccacg ccggcgtccg ggtggcgagg ggcggctcgg ccgtcggtcc
     2581 gtcgccggcg acggtggtgc ggcgcatcac gcggcggtgc cgcgcggcgt cccacgggcg
     2641 cccacggtgc agcacgcagc ggttgtccca catgacgagg tcccacgggc gccagcggtg
     2701 ctggtagacg cactcgggac gcgtggcgtg cgcgagcagg tcgtcgagca gccggcgcga
     2761 ctccgcgtag tccatgccgt cgacgtacca ggcgtgcgag cccacgaaga tggacttacg
     2821 cccgttcacc gggttgacgc gcacgagcgc ctggcggacg ggcggcaccc cctgctcctg
     2881 gcccgggtcg aagagcccgg gcgcgatcgt gtatctcgaa tagacgatgc tgtggaccgc
     2941 cacgcgcccc tcgatgagcc tccgcgtcgc gtcgggcagc gtcgcccacg cgtggcgcag
     3001 gctggcgaac tcggtctcgc cgccggccgg aggcacctcg cgaccggaga gcatcgacgc
     3061 catggcggga acgggcttga acgacgagtc gctgtgccag agctggttgc ccgactgata
     3121 gaccatgcgc cggtcgcccc agtccatcag gcggtcttcg gcctcgggat cgaggttcga
     3181 gaggtccacc agattcgggt gcaggcggcc ccccttgctg atcgagcgga tcgtcgtctc
     3241 gagcggtccg aagtgctcgc tgaacgccat ctgctgctcg tcggtcagcc gctggtcgcg
     3301 gaacaccagc acggagtgct cctggaaggc gtcgaagacc gcgccaaacg tcgcgtcgtc
     3361 cagaggccgc gcgagatcca cgccttccac cagcgccccg aggtgagggt ggatcgtcgt
     3421 gatcgtcggc tggcccgtca gggtggccat gggcacacct cccgctgacg ggaggataac
     3481 ggtggccggc ggcgacccgc aagcgccacc cacgtcgacc acctccgagc tgcgggagcc
     3541 gtgcggggcg ggccgcccgc gccgcgtgtg aacccggccg gccggtgcgg gggcggtgct
     3601 acaatcgcgc cctcggcacc acacacgcgg aggacgccat gagcgcactg agtcggcgga
     3661 cgctgttgaa gatgggcgcg ggtgctgccg tcctgggcgc accgggagtg ctccgggccc
     3721 agggcaagga cgtggtgatg ctgggtctct ggccgttcac cggcgccttc gcggacgtcg
     3781 ggcccatcct cgaccgcggc atgaagatgg cgctcgagga gcgcggcatg aaggccgcgg
     3841 ggcgcccgat caagtacgtc acgcgggact ccgagaccaa ggccggctcg gccacgcgac
     3901 gcgccgagga ggccgtggac ggcgagggcg cgaagtacat cattgggccc tggtcgtccg
     3961 gggtggcgct ggccgtctcc gaggtcgcca agcgcaagaa ggtcgtccac tacttctcgg
     4021 gcggcaccga ggacatctcg gggtcgcgct gccaccgcta ctcgttccag tgggcggcca
     4081 gcccgtacac ggcggccaag accgtcgtgg acaacttcat gaaagccaac cccaaggcca
     4141 agcgctggca cctgctggtc gccgactacg ccttcggctg gtccgtcgag aagtacatca
     4201 aggaggtcgg caaggaccac gggctggagt tcgtcggtgc cgaccgccat ccgctcggcg
     4261 agcgcgagtt ctccaactac gtcaccaagg ccgccggcaa caagcccgac gccgtggcca
     4321 tgatcaactt cgggctcgac gcggtgaccg ccgcgcgcga gatcttcaac ttcggactca
     4381 cgccgaaggt ccctctgatc atgacgtggt cgtcgggcgt cgaggagttg atccagctcg
     4441 cgcccgagat ccgcgagaac acctgggtcg gctcgaactt ctactacacc gccgacaccc
     4501 cggtggcgcg cgcgttcgtg aagagctacc aggagaagca caagaacccg cccggctatg
     4561 cgccggcggc ggcctacggg atgacgcgca tggtcctggc cgccatggag aaggccagat
     4621 ctgccgaggt ccccgaagtc atcgcggccc tcgagggcct cgaggtcgac gacctggtcg
     4681 gccggatgcg ggtcgagccc aagacccacc agacgctgcg gccctacttc ttcatgcgct
     4741 gcaagaagaa ggacgcgatg aagcacccga tggacttcgc ggacatcatc gccacgggct
     4801 cgacgccgct gccggcgcag tacagcgcct gcaaggacat cggcgcggtc tagtaacatg
     4861 ggggccccga catggccccc aagcccccaa cgttcggcac gccccggcgc agccgtggcg
     4921 cgcctctatc ccgcgcgagg tgttgggctc gacatggggg ccctgacatg gcccccaagc
     4981 ccccaacgtt cggcacgccc cggcgcagcc gtggcgcgcc tctatcccgc gcgaggtgtc
     5041 gggtcccacg atgaacgcgg gcctgttgtt cggtcagacc ctgaacggcg tggcgacggg
     5101 gatgctgtac gccctgatgg ggatcgggct cagcatcgtc accggggtgc tgaacatccc
     5161 gaactttgcg cacggcgcca tgttcgccct cggcgcctac ctgctcttcg cggcgatcca
     5221 ggtcgtggga aacttctggc tcgcgctggt gctggcgccg gtcggcgtcc tcgcgctcgg
     5281 gcttgtcgtc gagtacgccg gcatccggcc gctctaccgc gccggccacg actaccagct
     5341 cctgctcacc ttcgggctct ccctgatcgt caccgagggc atcatcgtcg tctgggggcc
     5401 gatcggcatg agccagctcc cgccggacat cctccgcgga ggcgtcgatc tcggggtcac
     5461 gatctacccg aagtaccggc tgttcgtcat gggcatggcg gggctgctgg tggcgggctc
     5521 ctggctgttc ctcgagaaga cgcgctacgg ggccatcatg cgcgccggca tcgaggacag
     5581 ggagatggtc gcgctgctcg gcatcgacat ccaccggctc ttcacggcgg cctttggcct
     5641 cggcgcgtat ctcgccggca tcgcgggagc gctgaccgcg ccgatccggg gcctgaaccc
     5701 gttcatgggc gtggacatgc tcgggatcgc cttcgtggtg gtcgcgctcg gcggcctcgg
     5761 caacctcccc ggcgccatcg tggccggcat catcgtcggc gtggcccaga gcctggtgtc
     5821 gctcgtctgg ccggaggcct cggtcgccgt catcttcgcg gtgatggccg cggtcctgct
     5881 cgtccgcccc cagggattgt tcggcatccg atgacgccac gccctcccgt gtcacgctcc
     5941 tcgccgtggc gccggccgcg cggcttccac cacgaatgat ccgcgcctgg ctcgccgtgc
     6001 tcgccgccgt cgtcctgctg cccctcgtcg tccgccacgc catcgccacc gagatctgga
     6061 tcttcgcgat cttcggcctc gggctgaatc tcctgctcgg ctacacgggg ttgctctcgt
     6121 tcgggcaggc gacattcttc ggctcggccg cctacgtcgg cggctacctg ttgaagcatc
     6181 acggcgtccc cgtcctcgcc gtgctggcga tcggtctcgc ggtcggcgcc gtgtccgcgg
     6241 ccctcgtcgg ctatctctgc gtacagcgct cgggcctcta cttcatcatg ctgaccttcg
     6301 cgctgaacca gatgttcttc ttcaccgcct accagtggac gtcggtcacc ggcggcgagg
     6361 acggcatgcc gggcgttccg cgcccgccgc tcctcggcct cgacctcaac gggacgctca
     6421 cctattacgc cttcgtcacc gccctctttc tgatgtcgct ctgggtcatg cagcgcatcg
     6481 tggcttcccc gctcggcgtg atcctccagg ccatccgcga gaacgaggtg cgcgccgagg
     6541 ccgtgggctt cgacgcccgg cgcttcaagc tgctggcgtt cgtgatcggc ggggccttct
     6601 ccggagtcgc cggcgtcctc tacgcgatgc tcttcggcat cgtgccgctc gaggccatcg
     6661 gcttcgtgtt ctccggcaac gtggtcttcg ccacgctcat cggcggctcg ggctcgctct
     6721 acggcccgat catcggcagc ttcatcttca tctggctgtc ggagtcggtt agcgtgatct
     6781 ggcagcgctg gccgctcctg ctgggcgtcg ccttcgtcat cgtcgtcctc ttcttccgcg
     6841 gcggcgtcgt ggaggcgtgg gaccggctcc ggctctggag gacgcgccgt ggcgctgctg
     6901 aggactgaag gactcaccaa gaccttcggc gcgctcacgg ccgtgagcga cgtgtcgctg
     6961 gcggtcgagg ccggcacgct gcactcgatc atcggcccga acggcgccgg caagacgacg
     7021 ctcttcaacc tgctcacggg ccagcacgcg ccgaccgccg gccgcatcgt cctggacggc
     7081 cgcgacatcg ccggcacgcc gccccagcgc atcgcgcacc tcggcgtggc gcggtcgttc
     7141 cagcgcacca acgtcttccc cgcgctgacg gtgctggaca acgtgtgggt ggcggcgttc
     7201 gcgcgcgggt cgtggcgcgg gctggcgtgg cggccggctg cccgctacgc cgaccggcgc
     7261 gagcgcgcgc tgacggcgct gcgcgaggtg ggcctcgccg acaaggccgg ccaccgggcg
     7321 cgcgagatct cgcacggcga gcagcggcag ctggagctgg ccatcgcgct ggccgccacg
     7381 ccccgcctgc tgctgctcga cgagcccgcg gccggattga gccccgacga gacgcggaag
     7441 atggtgcagc ttgtccgcgc cctcaagggc cggtacacga tcgtgctggt ggagcacaag
     7501 atggacgtca tcatgacgat ctcggaccgc atctcggtga tgcacttcgg ccggctcatc
     7561 gccgagggca ccccggccga gatccgggag caccccgagg tgcgccgggc ctacctcggc
     7621 gggatggcgg cctcgtgatc ctcgacgtcc gccgcatcga cgcgcactac ggcctcgccc
     7681 acgtgctcca cggcctgtcc ctgcgcgtgg aggagggcga ggtggtggcg ctgctcgggc
     7741 gcaacggcgc cggcaagacc accacgatgc gcgcgatcac gggtctgacg cccccgcggg
     7801 ccggcgacat cctctacagg ggctcgagca tcgccgggcg cgccgcgcac cacatcgcgc
     7861 gccttggcat cgcgctggtg ccggagacgc ggggcatctt ctcctacctc accgcccgcg
     7921 agaacctgga catcgcgcgg cgccccgggt cgcgctggag ccgcgagatc gtgctcgagc
     7981 gcttccccaa gctccgcgag gtcctcgacc gcaagggccg gttcctctcg ggcggcgagc
     8041 agcagatgct cgccatcgcg cgcgccctga tgaccggccc cgacctgttg ctgctcgacg
     8101 agccctcgca gggactggcc ccgctggtcg tcgagaccgt catgcagacc atcgccgagc
     8161 tcaagcgcga gcgggtgagc atgctgctcg tcgagcagaa cgccgagctg gccctgcgcc
     8221 tcgccgaccg cgtctatgtg atcgaccacg gcacgatcgt cttcgagggc acgccggcgt
     8281 cgctgcgcgc ggatgtgcgg gtgacgtcga cctacctggg
//
LOCUS       DATIAZ010000034         5501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1586316, whole genome shotgun sequence.
ACCESSION   DATIAZ010000034 DATIAZ010000000
VERSION     DATIAZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586316"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VLF19_01185"
     CDS             complement(<1..137)
                     /locus_tag="VLF19_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HSE91890.1"
                     /translation="MTEGLMANIPGDLRYTREHEWAKKDGANVRVGITHFAQEQLGDV
                     V"
     gene            complement(316..1416)
                     /gene="gcvT"
                     /locus_tag="VLF19_01190"
     CDS             complement(316..1416)
                     /gene="gcvT"
                     /locus_tag="VLF19_01190"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HSE91891.1"
                     /translation="MLKRTPLHDVHVKAGARMVPFGGWDMPVQYTSILEEHRRVRAAV
                     GLFDVSHMGEFEVEGAEALAALQHLTTNDVAALEVGQVQYSLLCDPDGGIVDDLTLYR
                     LAPERYMLTVNASNTDKDWAWVTGESHRFRGARWRNTSAETGLVAVQGPRAEALVGRL
                     ADRDVSAIGYYRFAPRTVGGVATLASRTGYTGEDGFELYVATGETVRLWHALVEAGAA
                     DGVGPAGLGARDTLRLEMRYALYGNDIDQTTNPLEAGLGWVVKPAKGEFIGRAAIERV
                     RAAGPRRRLVGLEMADRAVARHGYLVVRDARAVGVVTSGSYGPSVEKSIALAYVESAL
                     AAAGTELGVDVRGQARPARVVKTPFYPSRVKK"
     gene            complement(1526..2320)
                     /locus_tag="VLF19_01195"
     CDS             complement(1526..2320)
                     /locus_tag="VLF19_01195"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HSE91892.1"
                     /translation="MAAQKFIDLNCDMGESYGRWTLGNDAEIMPFITSANVACGFHGG
                     DPHVMRATVELALRHGVAVGSHPSLPDLMGFGRRVMDVSPQELKDYLHYQSGALREFL
                     RAGGSELQHVKPHGILYTMMERHEYEALGRACGEAVQEARDPKLILMTLAAGKCDQAC
                     RKMGVRVASEGFADRAYNVDLTLVSRKIQGSLITDPQRAAAQAVKMAMEGKVKTLDGV
                     EVDLAVQTICCHGDTPGSDRIVRAVREALEKAGCQVKPLRDWLPAV"
     gene            complement(2342..3667)
                     /locus_tag="VLF19_01200"
     CDS             complement(2342..3667)
                     /locus_tag="VLF19_01200"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSE91893.1"
                     /translation="MAIDKELNEYLAKTSRSRSMHEEATAVMPGGNSRTTTFFDPYPF
                     YIQRGQGAHVWDADGVDRLDFNGNYTSLVLGHAPPDVVKAAQEAAAQGMSFPGPTEHE
                     IRLAEMLVRRLPSVETIRFTNSGTEATMNAVRAARAFTGRPKIAKFEGAYHGTHDWVL
                     VSVAPDPKAAGSRKHPKPVPASSGVPPTVLKHTVVLPWNDPDACQALLAKEADSLACL
                     LVDPLLGIGGVLPPADGFLQSLREVTARHGIVLIFDEVISFRIAWGGAQERYGVAPDL
                     TTLGKIIGGGLPVGAFGGRGDIMAAYDPRRGGARISHGGTFNANPVTMAAGAATLNAL
                     TPEAYARLDALGDRLRGGVSRLLQNTRRRGQVTGVGSLFCLHWTSGPLTDYRSSRPKD
                     PEAPIRVFMGLLNEGILLTQRGLGACSLAMEDEDVDRFVNALARVLARE"
     gene            complement(3681..5354)
                     /gene="pxpB"
                     /locus_tag="VLF19_01205"
     CDS             complement(3681..5354)
                     /gene="pxpB"
                     /locus_tag="VLF19_01205"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HSE91894.1"
                     /translation="MRFFPAGDLAVSVELDEEISVEVNTRVRALEFLIQQKGLAGVVE
                     TVPAFRTLLVYYDTLVTEYDELCAALSELAAAAAQATLPPARTVELPCCYDDPELGVD
                     LAPAAQRLGLTPEELVRLHAGAEYLVYFLGFTPGLPYMFGMPERIHLPRLETPRTKVP
                     PGSVGIGGAQCCIYSVESPGGYWLLGRTPLRLYAPDTPEPILLRPGDRVRFRPIDRTE
                     YEAIAAEVSASSAGMVGASDGGRPRNGDEAPVRVIEPGVQTTVQDLGRPAQLRYGIPP
                     SGPMDRWSFVVANRLVGNPDTAAGLECAYMGPRFQVDRDCAMAVTGAPAPVTVNGQEA
                     PRWTTVVLRAGDTVRVGPPRTGVLSYVAFSGGIDVPLVLGSRSTYVRGRLGGLQGRAL
                     RRDDVLRLGDAPLPATCSLATPPSIGGEEETVVRVVLGPQADRFTEKGIQTLLGSAYE
                     MQPQSDRMGARLRGPRISHSRGHDIISDGIALGSIQVPGDGQPIALLVDRQSTGGYTK
                     VATICSFDIGRLGQVKPGQRVRFEALALADAHRTLRHWQASLAGVLAPR"
     gene            complement(5373..>5501)
                     /locus_tag="VLF19_01210"
     CDS             complement(5373..>5501)
                     /locus_tag="VLF19_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013057187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1445 domain-containing protein"
                     /protein_id="HSE91895.1"
                     /translation="VFWACGVTPQAVALASKPPFMITHSPGHMFVTDVPNSALAVL"
BASE COUNT          772 a         1973 c         1953 g          803 t
ORIGIN      
        1 aagacgacgt cgccgagctg ctcctgcgcg aagtgcgtga tgccgacgcg gacgttggcg
       61 ccgtccttct tggcccactc gtgctcgcgc gtgtacctga gatcgcccgg gatgttcgcc
      121 atgagtccct ctgtcatctt acttgccgtc ggagcgcctt cgcagggctc cggcgctcga
      181 cggtcggccg ggtgccgcgc tccatcatgt cccgttgccg tcggcgcgcc ttcgcagggc
      241 tccggcgctc gacggtcggc ccgcgccgcg ctcgatcatg tcccgttgcc gcgccggagc
      301 accgcacgtg ctcggtcact tcttgacccg tgacgggtag aacggggtct tgacgacgcg
      361 ggccggccgg gcctgcccgc gcacgtccac accgagctcg gtgccggcgg cggcgagcgc
      421 gctctcgacg taggccagcg cgatcgattt ttcgacggac ggcccgtagg agccggacgt
      481 caccacgccg acggcgcggg cgtccctcac caccagatag ccgtggcgcg cgacggcccg
      541 gtcggccatc tcgagtccga cgagccgccg ccgcggcccg gcggcccgca cgcgctcgat
      601 cgccgcgcgc ccgatgaact cgcccttggc gggcttcacg acccatccca acccggcctc
      661 gagcggattc gtcgtctggt cgatgtcgtt gccgtacagc gcgtagcgca tctccagccg
      721 caaggtgtca cgggccccga gtccggccgg gcccacgccg tccgcggcgc cggcctccac
      781 gagcgcgtgc cacaggcgaa cggtctcgcc ggtggcgacg tacagctcga agccgtcctc
      841 cccggtgtag ccggtgcgcg aggcgagcgt ggcgacgccg cccacggtgc gcggcgcgaa
      901 gcgatagtaa ccgatcgcgg acacgtcacg gtcggccagg cgcccgacca gcgcctcggc
      961 gcgcgggccc tgcacggcga cgaggcccgt ctccgccgac gtgttgcgcc agcgcgcgcc
     1021 ccggaagcgg tgcgattccc cggtgaccca cgcccagtcc ttgtcggtgt tcgacgcgtt
     1081 gacggtcagc atgtagcgct cgggggccag gcgatacagc gtgaggtcgt cgacgatgcc
     1141 gccgtcggga tcgcagagca acgagtactg gacctgcccg acctccagcg cggccacgtc
     1201 gttggtggtg aggtgctgca gcgcggcaag cgcttcggcc ccctcgacct cgaactcgcc
     1261 catgtgactc acgtcgaaca ggccgacggc cgcgcgcacg cgacggtgct cctcgaggat
     1321 cgacgtgtac tgcaccggca tgtcccagcc gccgaagggg accatgcggg cgccggcctt
     1381 cacgtggacg tcgtgcagag gggtgcgctt gagcagcgtc tccatcgagg cgcctcagta
     1441 gaacatcgcc cccgcagagg gtcaagagcc ggccggcagc gcctttgctc cggtcccgtc
     1501 gtcggcaaac cttgcggtgc ggactctaga ccgctggcaa ccagtcgcgc agcggcttca
     1561 cctgacagcc cgccttctcg agcgcctccc gcaccgcgcg gacgatgcgg tcagaacccg
     1621 gagtgtcgcc gtggcagcag atcgtctgga cggcgaggtc gacctccacg ccgtcgagcg
     1681 ttttcacctt gccttccatc gccatcttca cggcctgggc ggcggccctc tgtggatcgg
     1741 tgatgagcga gccctggatc ttgcgcgaga cgagagtgag gtcgacgttg tacgcgcggt
     1801 cggcgaagcc ctcggaggcg acgcgcacgc ccatcttccg gcacgcctgg tcgcacttgc
     1861 cggcggcgag cgtcatgagg atgagcttgg ggtcgcgcgc ttcctgcacc gcctcgccgc
     1921 aggcccgccc gagggcctcg tactcgtgcc gctccatcat cgtgtagagg atcccgtgcg
     1981 gcttgacgtg ctgcagctcg gagccccccg cgcggaggaa ctcgcgcagc gcgcccgact
     2041 ggtagtggag gtagtccttc agctcctggg gtgagacgtc catcacgcgc cggccgaaac
     2101 ccatgagatc gggcagcgag gggtgggagc ccacggcgac gccgtgccgg agcgcgagct
     2161 ccaccgtggc gcgcatcacg tgcgggtcgc ccccgtggaa gccgcaggcc acgttcgccg
     2221 aggtgatgaa gggcatgatc tcggcgtcgt tgccgagcgt ccaccggccg tagctctcgc
     2281 ccatgtcgca gttgaggtcg atgaatttct gggctgccat gaatggatct cctttcgccg
     2341 tctactcgcg cgccaggacc ctcgccagcg cgttgacgaa gcggtcgacg tcctcgtcct
     2401 ccatcgcgag cgagcaggcg ccgaggcccc gctgggtgag gaggatgccc tcgttgagga
     2461 ggcccatgaa cacgcggatc ggcgcctccg ggtccttggg ccggctcgag cgatagtcgg
     2521 tgagcgggcc cgacgtccag tgcaagcaga acagcgagcc gacgcccgtg acctggccgc
     2581 ggcgccgcgt gttctgcagc agacgcgaca cgccgccacg caggcggtcg cccagtgcgt
     2641 cgagcctcgc gtaggcctcg ggcgtgagcg cgttgagcgt cgccgcgccg gcggccatgg
     2701 tcaccgggtt cgcgttgaaa gtgccgccat ggctgatgcg cgcgccgccg cgccgcgggt
     2761 cgtaggcggc catgatgtcc ccgcgcccgc cgaaggcgcc gacgggaagg ccgccgccga
     2821 tgatcttgcc gagcgtggtg aggtccgggg cgacgccgta ccgctcctgc gcgccgcccc
     2881 acgcgatccg gaacgagatg acctcgtcga agatcagcac gatgccgtgc ctcgccgtca
     2941 cctcgcgcag ggattgcagg aacccgtcgg ccgggggcag gacgccgccg ataccgagca
     3001 gcgggtcgac gaggaggcac gccaggctgt ccgcctcctt cgcgagcagc gcctggcacg
     3061 catcgggatc gttccacggc agcaccacgg tgtgcttgag caccgtcggg ggcacccccg
     3121 acgacgccgg cacgggcttg gggtgcttgc ggctgccggc cgccttcggg tcgggcgcga
     3181 cgctcacgag cacccagtcg tgggtgccgt gatacgcgcc ctcgaacttc gcgatcttcg
     3241 gccgacctgt gaacgcgcgg gcggcgcgca cggcgttcat ggtcgcctcg gtgccggagt
     3301 tcgtgaagcg gatcgtctcg accgacggca ggcggcgcac gagcatctcg gcgagccgga
     3361 tctcgtgctc ggtcgggccc gggaacgaca tgccctgcgc ggccgcctcc tgcgcggcct
     3421 tgacgacgtc ggggggcgcg tgacccagga cgaggctcgt gtaattgccg ttgaagtcca
     3481 gccggtcgac gccgtcggca tcccagacgt gggcgccctg cccgcgctgg atgtagaacg
     3541 gataggggtc gaagaacgtc gtggtgcggc tgttgccgcc gggcatgacc gcggtcgcct
     3601 cctcgtgcat cgagcgcgag cgcgacgtct tcgccaggta ctcgttcagc tccttgtcga
     3661 tcgccatccc cgttctcccg tcacctcggg gcgagcacgc ccgcgagcga ggcctgccag
     3721 tgtctgagtg tccgatgcgc atcggccagg gcgagcgcct cgaagcgcac ccgctgtccc
     3781 ggcttcacct ggccgaggcg accgatgtcg aacgagcata tggtcgccac cttcgtgtag
     3841 ccgccggtcg actgccggtc cacgagcagc gcgatcggct ggccgtcgcc gggcacctgg
     3901 atgctgccca gcgcgatgcc atccgagatg atatcgtgcc cgcgcgagtg gctgatgcgc
     3961 ggtccgcgca gccgcgcgcc catgcgatcc gactgcggct gcatctcgta cgccgagccg
     4021 agcagcgtct ggatgccctt ttcggtgaag cggtccgcct gcggccccag gacgacgcgc
     4081 acgaccgtct cctcttcgcc gccgatggag ggcggcgtgg ccagcgagca tgtcgccggc
     4141 agcggggcgt cgccgagacg caggacgtcg tcccggcgca gcgcgcgtcc ctgcaggccg
     4201 cccaggcggc ctcgcacata cgtcgagcgc gacccgagga cgagcggcac gtcgatcccg
     4261 ccggagaacg cgacgtagct gagcaccccc gtccgcgggg gtccgacacg caccgtgtcg
     4321 cccgcgcgga gcacgaccgt cgtccagcgg ggcgcctcct ggccgttgac cgtcaccggc
     4381 gccggcgcgc cggtcacggc catcgcgcag tcgcgatcca cctgaaagcg cggccccatg
     4441 tacgcgcact cgaggccggc cgccgtgtcc gggttgccga cgagccgatt ggcgaccacg
     4501 aacgaccacc ggtccatcgg gcccgagggt gggatgccgt agcgcagctg ggccggccgc
     4561 ccgaggtcct gcaccgtcgt ctgcacgccg ggctcgatca ctcggacggg ggcttcgtcc
     4621 ccgttccgag gcctcccccc atcacttgcc ccgaccatgc cggcgctcga agccgagacc
     4681 tcggccgcga tcgcttcgta ttcggtgcgg tcgatggggc ggaagcggac gcgatcgccc
     4741 gggcgcagga ggatgggctc gggggtgtcg ggggcgtaga gtctgagcgg cgtccgtccg
     4801 agcagccagt agccgccggg actctcgacg gagtagatgc agcactgcgc gccgccgatc
     4861 cccacgctgc ccggcggcac cttcgtacgc ggcgtctcga ggcgcggcag atggatgcgc
     4921 tcgggcatcc cgaacatgta gggcagcccc ggcgtgaagc cgaggaaata gacgaggtac
     4981 tcggccccgg cgtgcagccg caccagctcc tcgggggtga ggcccaggcg ctgcgccgcg
     5041 ggggcgaggt cgacgcccag ctccgggtcg tcgtagcagc acggcagctc gaccgtgcgc
     5101 gccggcggga gggtggcctg cgcggccgcc gccgccagct cgctgagcgc ggcgcagagc
     5161 tcgtcgtatt ccgtcacgag ggtatcgtag tacacgagca gcgtccggaa cgcgggaacg
     5221 gtctcgacga cgccggcgag ccctttctgc tggatcagga actcgagcgc gcgcacccgc
     5281 gtgttgacct cgacgctgat ctcctcgtcc agctcgacgg agacggcgag atcgcccgcg
     5341 gggaaaaagc gcagacgccg gcccctggac cgctagagga ccgccagcgc cgagttgggc
     5401 acgtcggtga cgaacatgtg ccccgggctg tgggtgatca tgaacggcgg cttcgaggcc
     5461 agcgcgaccg cctgaggcgt cacgccacag gcccagaaca c
//
LOCUS       DATIAZ010000035         3004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1849626, whole genome shotgun sequence.
ACCESSION   DATIAZ010000035 DATIAZ010000000
VERSION     DATIAZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849626"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1138)
                     /locus_tag="VLF19_01215"
     CDS             complement(350..1138)
                     /locus_tag="VLF19_01215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009636181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE91896.1"
                     /translation="MKTLDGKVAIVTGGGTGIGRAVALMLAAEGALVVVAGRRKAPLD
                     AVVREIEEAGGRGVAYQVDLVDATEAAALGRWAVQTHGRVDVLVNNAGQSSHVRNIRW
                     LPKDEWDSVLGVNLTGVFALTQAVLGKMLEQGGGTVVTVASAAALRSGLLSGAAYGAA
                     KAGARNLMTHLHNVFRNQGIRATTIIPAEVDTPILDKRPLPPDAAARATMMQPEDVAR
                     AILLCATLPPRTVIEEIVISPTVLRDQSQDIAFNRDLGAPQARA"
     gene            complement(1146..1745)
                     /locus_tag="VLF19_01220"
     CDS             complement(1146..1745)
                     /locus_tag="VLF19_01220"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HSE91897.1"
                     /translation="MRQRAAAHLAAFERRVAGVNGHRPAAVAAVLLPDGVGRACLLIT
                     RRAATLRSHTRQWALPGGRIESDETPVGAALRELREEVGLALGESAVLGLLDDYPTRS
                     GYVITPVVVWAGGDVVLEPSPDEVASVHRVPLDDLDAPEVPRLVRIPESDRPVIQLPI
                     LSTLVHAPTAAVVYQVREVVVHGRPTRVDHFEQPVWAWR"
     gene            complement(1772..2062)
                     /locus_tag="VLF19_01225"
     CDS             complement(1772..2062)
                     /locus_tag="VLF19_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HSE91898.1"
                     /translation="MPAYVIGDIEVTDAAGFEEYRKQVPATVQKYGGRFLVRGGAMEV
                     VEGDWRPKRVVVLEFPSMEQAKRWYDSEEYRAPKAVRLKTSRGRIILVEGTS"
     gene            complement(2064..2417)
                     /locus_tag="VLF19_01230"
     CDS             complement(2064..2417)
                     /locus_tag="VLF19_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91899.1"
                     /translation="MPITTRYLFSAAMDVAPDREAIFNEVYDHEHVPLLLKVPGVVSV
                     ARFTLEPLTMILGGERKTIVVQDQPRYQALYELETPDVLVSEPWAKAVDQGRWPGEVR
                     PHTKNRRHVLYRRIP"
     gene            complement(2542..>3004)
                     /locus_tag="VLF19_01235"
     CDS             complement(2542..>3004)
                     /locus_tag="VLF19_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HSE91900.1"
                     /translation="RCDVSPKGDPAGFVLVLDDHHLVIPDRPGNRRFDGMRNILANPH
                     IGLLFLVPARGETLRVNGRASIIRDAEVLARLAVEGKTPRLAIGVEVEEVFLHCAKAF
                     RRSSLWRPEAWPDISTLPSAACMLLAHARPEGMTLEQMEHRLRHSYDTQLY"
BASE COUNT          408 a         1077 c         1074 g          445 t
ORIGIN      
        1 ccagcgcccg accccggccg atcgcacggt gacgcgcccg tcgaccccac gctcgagcag
       61 catccgctcg agcaggagct gcgccatcac gctgcgcgcc gtgttggcct ggcagacgag
      121 caggatacgc accgctcaga gtccgtcgac gaatcgcgtt gtcgaggagt gccaccgctg
      181 tgccggggca cttccgggcg tctggccggg cgagtccgag ggggcgtggc ccacccatcg
      241 ctcgaagacc gtgggtggcc atgtcggggc cctcatgtca ccagcagccg gggactcggc
      301 tcgcctgctc ggctggcgcg gcccggctcg ctcgggtcgc tcggcgcgct catgcccgtg
      361 cctgcggggc cccgaggtcg cggttgaatg cgatgtcctg gctctggtcg cgcaggacgg
      421 tcggcgagat cacgatctcc tcgatcaccg tgcgcggcgg cagcgtcgcg cacaggagga
      481 tggcgcgcgc cacgtcctcg ggctgcatca tggtcgcgcg agcggcggcg tccggcggca
      541 ggggacgctt gtcgaggatg ggggtgtcca cctccgcggg gatgatcgtc gtcgcgcgga
      601 tcccctggtt gcgaaatacg ttgtggaggt gggtcatgag gttgcgcgcg ccggccttgg
      661 cggcaccgta ggccgcgccg ctcaggagac ccgagcgcag cgcagccgcg gaggcgacgg
      721 tcacgaccgt gccgccgccc tgctcgagca tcttgccgag caccgcctgg gtcagcgcga
      781 agacgccggt caagttcacc ccgagcacgc tgtcccactc gtccttcggc agccagcgga
      841 tgttgcgcac gtggctcgac tggccggcgt tgttgacgag gacgtccacg cgcccgtgcg
      901 tctggaccgc ccaccgtccg agcgccgccg cctcggtcgc gtcgacgagg tccacctggt
      961 aggcgacgcc gcggccaccg gcctcctcga tctcgcggac gacggcgtcg agcggcgcct
     1021 tccgccgccc ggcgacgacg accagcgcgc cctcggccgc cagcatgagc gccacggccc
     1081 gacctatgcc cgtcccccca cccgtgacga ttgcgacctt cccgtcgagt gtcttcatgt
     1141 cggagctatc tccacgccca cacgggctgc tcgaagtggt cgacgcgggt gggccggccg
     1201 tgcacgacga cctcgcgcac ctggtagacg accgccgccg tcggcgcgtg gaccagcgtg
     1261 gagaggatcg gcagctggat caccggccgg tcgctctccg ggatccgcac gagcctcggc
     1321 acctccggcg cgtcgaggtc gtcgagcggc acacgatgga cgctcgcgac ctcgtccgga
     1381 ctcggctcca ggacgacgtc gccgcccgcc cacacgacga cgggcgtgat cacatagccc
     1441 gagcgggtcg ggtagtcgtc gaggagtccg aggacggcgc tctcgccgag cgcgagcccc
     1501 acttcctccc ggagctcgcg cagcgccgct cccactggcg tctcgtcgga ctcgatgcga
     1561 ccgccgggca gcgcccactg gcgcgtgtgc gaacggagcg tcgccgcccg ccgggtgatc
     1621 agcaggcacg cccggccgac gccgtccggc aggagcaccg cggccacggc cgccggccgg
     1681 tggccgttca ctccggcgac gcggcgctcg aacgcggcca ggtgggcggc ggcccgctgg
     1741 cgcaacgcct cgtcgaaccg gtgggatgaa gtcaggaggt gccctcgacg aggatgatac
     1801 gcccgcgcga cgtcttcagg cgcaccgcct tcggcgcgcg gtactcctcg gagtcgtacc
     1861 agcgcttggc ctgctccatg ctcgggaact cgagcaccac cacgcgcttg ggcctccagt
     1921 ccccttccac gacttccatc gccccgccgc ggacgaggaa tcggccgccg tacttctgga
     1981 cggtcgcggg tacctgcttc cggtactcct cgaacccggc cgcgtccgtg acctcgatgt
     2041 cgccgatgac gtaggccggc atgtcagggg atcctccggt agaggacgtg ccgccggttc
     2101 ttcgtgtggg gccgcacctc gccgggccag cgcccctggt ccacggcctt ggcccacggc
     2161 tcgctcacga ggacatccgg ggtctccagc tcgtacaggg cctgatagcg gggctggtcc
     2221 tggacgacga tggtctttcg ctcgcccccc aggatcatcg tgagcggctc gagcgtgaag
     2281 cgcgccaccg agacgacgcc gggcaccttc agcagcagcg gcacgtgctc gtggtcgtag
     2341 acctcgttga agatcgcctc gcggtctggc gcgacgtcca tggcggcgct gaagagatag
     2401 cgggtcgtga tcggcatcgc tctcctcctt ccgggcccag agcccgtgta cgaatcgcgc
     2461 tgtcgaaggg cgctactgct ctgccgtaac gtttcgagcc acccgggggg cttggggggc
     2521 gcccggagcc cccccatgta ttcaataaag ttgggtgtcg taggagtgcc gcaggcggtg
     2581 ctccatctgc tcgagtgtca tgccctccgg ccgcgcgtgt gccagcagca tgcaggccgc
     2641 ggagggcagc gtgctgatgt ccggccacgc ctcggggcgc cagaggctcg agcgcctgaa
     2701 ggccttggcg cagtgcagga agacctcctc cacctcgaca ccgatcgcca ggcggggcgt
     2761 cttgccctcg acggccaggc gggcgagcac ctcggcgtcg cggatgatcg aggcgcggcc
     2821 gttcacgcgg agggtctcgc cgcgcgcggg cacgaggaag agcagcccga tgtgcgggtt
     2881 ggcgaggatg ttgcgcatgc cgtcgaaccg gcgattgccc ggccggtcgg ggatgacgag
     2941 gtggtggtcg tccaggacga ggacgaagcc ggcagggtcg cccttgggcg acacgtcgca
     3001 gcgg
//
LOCUS       DATIAZ010000036         5313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_929512, whole genome shotgun sequence.
ACCESSION   DATIAZ010000036 DATIAZ010000000
VERSION     DATIAZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929512"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VLF19_01240"
     CDS             complement(<1..860)
                     /locus_tag="VLF19_01240"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSE91901.1"
                     /translation="MSAITGVFAREILDSRGFPTVEVEVQLESGAWGRAAVPSGASTG
                     SREAIELRDGDRSRYGAKGVLKAVEFVNTEISEAIVGLDATEQAFVDRTLIDLDGTEN
                     KSRLGANAILAVSMATARAAAEESGLPLYRYLGGSGPMRMPVPMMNVVNGGAHANNNL
                     DFQELMIVPVGQQSFREALRCGSEVFQALKKILESKNLPTAVGDEGGFAPDMPSHAAA
                     IELVIEAIGKAGYNAGQDVVLALDCAASEFFKDGSYTVEDKKLSSAQMIDYLAQLAGK
                     YPIVSIEDGMA"
     gene            complement(862..1755)
                     /locus_tag="VLF19_01245"
     CDS             complement(862..1755)
                     /locus_tag="VLF19_01245"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HSE91902.1"
                     /translation="MTFDLQHYLGARRELVDEALERFLPPDDAPPPAVHRAMRYSVMA
                     GGKRLRPILVIAGAEAVGGGSDTVLPTACALELIHTYSLIHDDLPAMDDDDYRRGRPT
                     SHKVFGEAMAILAGDALLTLAFRLVADNATLVRDPGTICAVVAEIATAAGTGGMVGGQ
                     VVDIESEGKTVTAETLDYIHTHKTAALIRVALRSGALLAGAPPAELQAITTTGERLGL
                     AFQIVDDILDVEGSLEELGKTSGSDERKQKATYPALHGLDASRRRARTLIQDAKDALT
                     PLGRAAAPIEALADFIFERRS"
     gene            complement(1752..1997)
                     /locus_tag="VLF19_01250"
     CDS             complement(1752..1997)
                     /locus_tag="VLF19_01250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HSE91903.1"
                     /translation="MTDLKFEDCLTRLEQIVGALESGNLALEESLKVFEEGVALARHC
                     ARYLDDAERRIELLTRDEGGGLAARPFTDWEREGEAG"
     gene            complement(1978..3267)
                     /gene="xseA"
                     /locus_tag="VLF19_01255"
     CDS             complement(1978..3267)
                     /gene="xseA"
                     /locus_tag="VLF19_01255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSE91904.1"
                     /translation="MSAGRAVLTVSQLTSQLRGVVEERFPAVWVEGEVSNFRLYGSGH
                     AYFTLKDEGAQLRAVLFRNRMRRLRFEPGDGQHLLAFGALEVYAQRGEYQLVVELLEP
                     RGFGALQLAFEQLKRRLTAEGLFDAARKRPLPRFPRKIGIATSPSGAAVRDMLRVIGR
                     RFGGLSIVVAPCRVQGEGAAQEIAEAIAALNALGDVDVIIVGRGGGSLEDLWAFNEET
                     VARAIAGSKAPVVSAVGHEVDVTIADFVADVRAATPSNAAELVVREKQAVADALGALR
                     RRLDRAVARPLGDLARRVDDARARLTRAGAAGHRRLGHRLELLSARLHAASPFARLAS
                     HRHRLERAASRLDGLMTRRLTAGRHRLGAAVGRLDSLSPLAVLGRGYSLTRTADGAIV
                     RRAVQVDAGDAVSVLLHEGSLLCRVERTEERDDRPQV"
     gene            complement(3264..4145)
                     /locus_tag="VLF19_01260"
     CDS             complement(3264..4145)
                     /locus_tag="VLF19_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HSE91905.1"
                     /translation="MILDGKVVREKVLARVREGAQRLRAARGVMPCLAVVLVGDDPAS
                     RSYVASKKRTAESVGIETRDHVYPTGLDQAALVRLLRQLDDDASIHAILLQLPLPAGM
                     DEAAALAAIAPEKDVDGLHPMNLGKLLAGEPGTVPCTPAGCIEILDHYGAELSGAEAV
                     VVGRSRLVGKPLAQLLLARHATVTTCHTRTRDLAAHVRRADVLCVAAGRPGIVTGDMV
                     KDGVWVIDVGFNRLPGGKAVGDVDFESVRRRARGITPVPGGVGPMTIAMLLENTLAAA
                     ERAPGGGPGHGPRSSTR"
     gene            complement(4195..4971)
                     /locus_tag="VLF19_01265"
     CDS             complement(4195..4971)
                     /locus_tag="VLF19_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HSE91906.1"
                     /translation="MNILMVGDVFGESGRAALRALLPGLRQQHAIDFTVVNVENAASG
                     FGVTPQQCRDFLDQGADVLTSGNHIWDRKEIVEFITRENLLLRPANFPAGTPGVGHVT
                     VKAGPHRVAVLNLMGRVFMHPIDCPFRKADEVVPELRRETPIVLVDMHAEATSESVAM
                     GWYLDGRVSAVVGTHRHVQTADERVLPGGTAYITDLGMTGPTDGVIGVDRDLILQRFL
                     QQMPIRFEPAKGPAALHGVVITVDPDTGRSTEIRRLRVPA"
     gene            complement(4979..>5313)
                     /locus_tag="VLF19_01270"
     CDS             complement(4979..>5313)
                     /locus_tag="VLF19_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HSE91907.1"
                     /translation="EPQTIEAVLTEAADGISAARPGARRDVLESYIKRLAKLEEIALS
                     YQGVEMCYAIQAGRELRVLTKADIVSDLDAHQLAKDITKRIEAEMQYPGHIKVVVIRE
                     TRAVEVAK"
BASE COUNT          712 a         1956 c         1882 g          763 t
ORIGIN      
        1 gccatgccgt cctcgatcga gacgatgggg tacttccccg ccagctgcgc aaggtagtcg
       61 atcatctgcg cggagctgag cttcttgtcc tccaccgtat aagagccgtc cttgaaaaac
      121 tctgaagcgg cgcagtccag cgccaggacg acgtcctgcc ccgcgttgta gcccgccttg
      181 ccgatcgcct cgatcaccag ctcgatcgcc gcggcgtggc tgggcatgtc cggcgcgaag
      241 ccgccctcgt cgccaacggc tgttggaagg ttcttgcttt cgagaatttt cttgagagcc
      301 tgaaagacct ctgacccgca cctcagtgct tcccggaagc tttgctggcc gacggggacg
      361 atcatcagct cctggaagtc gaggttgttg ttggcgtgcg cgccgccgtt caccacgttc
      421 atcatcggca ccggcatgcg catcgggccc gagcccccga ggtagcggta gagcggcagc
      481 ccggactcct cggccgccgc cctggcggtg gccatggaca cggcgaggat cgcgttcgcg
      541 cccaggcgcg acttgttctc cgtgccgtcg aggtcgatga gcgtcctgtc gacgaaggcc
      601 tgctcggtgg cgtccaggcc cacgatcgcc tccgagatct cggtattcac gaactccacc
      661 gccttcagca cccctttcgc gccgtagcgg ctgcggtcgc cgtcgcgcag ctcgatcgcc
      721 tcgcggctgc cggtggaggc ccccgagggc accgccgcgc ggccccaggc gccggattcg
      781 agctggacct cgacctcgac cgtgggaaag ccgcgagaat cgaggatttc gcgggcgaag
      841 acaccggtga tggcagacac gtcaggatct cctctcgaag atgaagtcgg ccagcgcctc
      901 gatcggcgcc gccgcgcgcc ccagcggggt cagcgcgtcc ttcgcgtcct ggatcagggt
      961 ccgggcgcgc cggcgggagg cgtcgaggcc gtgcagggcg ggataggtcg ccttctgctt
     1021 gcgctcgtcg ctaccggacg tcttccccag ctcctcgagg ctgccctcga cgtcgaggat
     1081 atcatcgacg atctggaacg cgagcccgag ccgctccccg gtcgtggtga tggcctggag
     1141 ctccgccggc ggggcgccgg ccagcagcgc gcccgagcgc agcgccacgc ggatgagcgc
     1201 cgccgtcttg tgggtgtgga tgtagtccag cgtctccgcg gtgacggtct tgccctcgga
     1261 ttcgatgtcc accacctgcc cgccgaccat gccgccggtg cccgcggccg tggcgatctc
     1321 cgccacgacg gcgcagatcg tgccggggtc gcgcacgagc gtggcgttgt cggcgacgag
     1381 ccggaaggcc agcgtgagca gcgcgtcgcc ggcgaggatc gccatcgcct cgccgaacac
     1441 cttgtggctc gtcggccggc cgcgccggta gtcgtcgtcg tccatcgccg gcaggtcgtc
     1501 gtggatcagc gagtacgtgt ggatcagctc gagcgcgcat gcggtgggca gcacggtgtc
     1561 gcttccgccg ccgacggcct cggcgccggc gatgacgagg atgggccgca gccgcttgcc
     1621 accggccatc acgctgtaac gcatcgcgcg atgcaccgcg ggcggcggcg cgtcgtcggg
     1681 cggcaggaag cgctcgagcg cctcgtccac gagctcccgg cgcgcaccga ggtagtgctg
     1741 gagatcgaag gtcatccggc ctcgccctcc cgctcccagt ccgtgaacgg ccgcgcggcc
     1801 aggcctccac cctcgtcgcg ggtcagcagc tcgatccgcc gctcggcgtc gtcgagatag
     1861 cgcgcgcagt ggcgggccag ggccacgccc tcctcgaaga ccttcagcga ctcctcgagc
     1921 gccaggtttc ccgactccag cgcgcccacg atctgctcga ggcgcgtcag gcagtcctca
     1981 aacttgaggt cggtcatccc gctcctccgt ccgctcgacg cggcacagga ggctcccctc
     2041 gtgcagcagc acgctcacgg cgtcgcccgc gtcgacctgc acggcgcgcc gcacgatcgc
     2101 gccgtccgcc gtgcgggtca ggctgtagcc gcggcccagc acggccagtg gcgacagcga
     2161 gtccaggcgg ccgacggccg cgccgagccg gtggcggccc gccgtcagcc gccgggtcat
     2221 gaggccgtcc aggcgcgagg ccgcacgctc gagacggtgc cggtgcgagg ccagccgcgc
     2281 gaacgggctc gccgcgtgca gccgtgcgct cagcagctcc aggcgatgcc cgagccgccg
     2341 gtggcccgcc gcgcccgccc gggtcagcct cgcccgcgca tcgtccacgc ggcgcgccag
     2401 gtccccgagc gggcgggcga cggcgcgatc gaggcggcgc cgcagcgcgc cgagcgcgtc
     2461 ggccaccgcc tgcttctccc gcaccaccag ctccgccgcg ttcgagggcg tggccgcgcg
     2521 cacgtcggcc acgaagtcgg cgatggtgac gtccacctcg tgccccaccg cggagaccac
     2581 cggcgctttc gagccggcga tggcgcgcgc caccgtctcc tcgttgaacg cccacaggtc
     2641 ctccagcgag ccgccgcccc ggcccacgat gatcacgtcc acgtcaccga gcgcattgag
     2701 ggcggcgatg gcctcggcga tctcctgcgc ggcgccctca ccctgcaccc ggcagggcgc
     2761 gacgacgatg gagaggccgc cgaaccggcg gccgatgacg cgcagcatgt cgcgcaccgc
     2821 ggcgccgctg ggcgaggtgg cgatgccgat cttgcgcggg aaccgtggga gcggccgctt
     2881 gcgggcggcg tcgaagaggc cctcggccgt gagccgccgc ttgagctgct cgaaggcgag
     2941 ctgcagcgcg ccgaacccgc gcggctccag cagctccacg acgagctggt actcgccgcg
     3001 ctgggcgtag acctcgagcg cgccgaatgc gagcaggtgc tggccgtcac ccggctcgaa
     3061 gcgcaggcgc cgcatgcggt tgcggaagag cacggcacgg agttgcgcgc cctcgtcctt
     3121 caaggtgaag tacgcgtgcc ccgacccgta cagccggaag ttcgacacct ccccctccac
     3181 ccagaccgcc gggaacctct cctccaccac cccccgcagc tgcgatgtca gctgcgacac
     3241 cgtcagcacc gccctccccg cgctcatcgc gtgctcgagc gcgggccgtg gccaggcccc
     3301 cctccggggg ctcgttcggc ggccgcgagc gtgttctcga gcagcatcgc gatggtcatc
     3361 ggccccacgc cgccgggcac gggggtgatg ccgcgcgcgc gccggcgcac cgactcgaag
     3421 tccacgtcgc ccacggcctt gccgccgggc agccggttga agccgacgtc gatcacccag
     3481 acgccgtcct tgaccatgtc gccggtgacg atgcccggcc ggcccgccgc cacgcacaag
     3541 acgtccgcgc gccggacgtg ggccgccagg tcgcgcgtgc gcgtgtggca ggtcgtcacc
     3601 gtggcgtgac gcgcgagcag cagctgcgcg agcggcttgc cgacgagccg cgagcgcccg
     3661 accacgaccg cctcggcccc ggacagctcg gcgccgtagt ggtcgaggat ctcgatgcat
     3721 ccggccggcg tgcagggcac ggtgcccggc tccccggcca gcagcttgcc caggttcatc
     3781 gggtgcagcc cgtccacgtc cttctcgggc gcgatggccg cgagcgcggc ggcctcgtcc
     3841 atccccgccg gcagcggcag ctggagcagg atcgcgtgga tcgacgcgtc gtcgtcgagc
     3901 tgccgcagca ggcggacgag cgcggcctgg tccaggccgg tcggatagac gtggtcccgc
     3961 gtctcgatgc ccacggactc ggccgtgcgc ttcttgctcg ccacgtacga ccgggacgcc
     4021 ggatcgtcgc cgacgaggac gacggccagg cacggcatga cgccgcgcgc ggcccggagg
     4081 cgctgggccc cctcgcggac gcgggccagc accttctctc ggaccacctt gccgtcgagg
     4141 atcacgcgtg gctcctgcgc gccggcgcga ggcgacgggc ggccgctcgg aatgtcacgc
     4201 cgggacgcga aggcgtcgga tctcggtgga gcgcccggtg tcggggtcga cggtgatgac
     4261 cacgccgtgc agcgcggccg gacccttggc cggctcgaag cggatgggca tctgctggag
     4321 gaagcgctgc aggatcaggt cgcggtccac gccgatcacc ccgtcggtgg gcccggtcat
     4381 gccgaggtcg gtgatgtacg cggtgccgcc cggcagcacc cgctcgtcgg cggtctgcac
     4441 gtggcggtgc gtgcccacga cggcgctgac gcgcccgtcg aggtaccagc ccatggcgac
     4501 cgactccgac gtcgcctcgg cgtgcatgtc cacgagcacg atcggcgtct cgcgccgcag
     4561 ctcgggcacg acctcgtcgg ccttgcggaa ggggcagtcg atggggtgca tgaagacccg
     4621 gcccatcagg ttcagcaccg ccacccggtg cggcccggcc ttgacggtga cgtgccccac
     4681 gcccggcgtg cccgccggga agttggccgg ccggaggagc aggttctccc tggtgatgaa
     4741 ctcgacgatc tccttgcggt cccagatgtg gttgcccgac gtcagcacgt cggcgccctg
     4801 gtcgaggaag tcgcggcact gctggggcgt gacgccgaag ccgctggcgg cgttctcgac
     4861 gttcaccacg gtgaagtcga tcgcgtgctg ctgccggagc cccggcagca gcgcgcgcag
     4921 agccgcgcgg cctgactcgc cgaagacgtc gccgaccatc aggatgttca tgcgcggcct
     4981 acttcgccac ctcgacggcg cgcgtctccc ggatgacgac gaccttgatg tggccggggt
     5041 actgcatctc ggcctcgatg cgtttggtga tgtccttggc gagctggtgg gcgtcgaggt
     5101 cggagacgat gtcggccttg gtgaggacgc gcagctcgcg gcccgcctgg atggcgtagc
     5161 acatctccac accctggtag gagagcgcga tctcctcgag cttggccagc cgcttgatgt
     5221 aggactccag cacgtcgcgg cgcgcgccgg ggcgggcggc ggagatgccg tcggcggcct
     5281 ccgtgaggac ggcctcgatc gtctgcggct cga
//
LOCUS       DATIAZ010000037         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_8860, whole genome shotgun sequence.
ACCESSION   DATIAZ010000037 DATIAZ010000000
VERSION     DATIAZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8860"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VLF19_01275"
     CDS             complement(<1..530)
                     /locus_tag="VLF19_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSE91908.1"
                     /translation="MPMQELTIRRREATGKQAAKRLRRAGAVPGVLYGGIKPEAVTVD
                     PRAVLRILHGHAGSTQLLTLAIEGDDGSRMAIIRDLQFDPVSDSLLHVDLQEVQADRA
                     ITVRVAVHPVGEAAGVKEQKGILQIVLHELEVSCLPTRIPERIDADVSPLMIGHVLTV
                     GDLAVPEGVRILNDRG"
     gene            complement(544..1488)
                     /locus_tag="VLF19_01280"
     CDS             complement(544..1488)
                     /locus_tag="VLF19_01280"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSE91909.1"
                     /translation="MTYDLKLFSGNAHRVLADEIAEYLKLPLGDVEVSRFSDGEVFVQ
                     VNENVRGTDVFVIQPTCPPVNDNLMELLVMIDAFKRASARRITAVLPYYGYARQDRKV
                     QPRVPITAKLVADLLTAAGVDRVLALDLHAGQIQGFFNIPVDHLFAGPVVMIDYLRGK
                     DLRDPVIISPDAGGVERARAIAKRFSAGLAIIDKRREGPNSAVAMHLIGDVEGRDAVV
                     IDDMIDTAGTLIQAVGAIQREGARRIIACGIHAVLSGPAIDRIKASPIEEVIVTNSIP
                     IADGKRAAGRITVLTVAPLLGEAIRRIHDEESVSTLFV"
     gene            complement(1508..1582)
                     /locus_tag="VLF19_01285"
     tRNA            complement(1508..1582)
                     /locus_tag="VLF19_01285"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1548..1550),aa:Gln,seq:ttg)
     gene            complement(1693..>2177)
                     /locus_tag="VLF19_01290"
     CDS             complement(1693..>2177)
                     /locus_tag="VLF19_01290"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HSE91910.1"
                     /translation="EIAVTLGMDVPFFLRGGTALATGRGEKLEPLPGVGLALVLVNPR
                     FGSSTAEVYGRVTPEMYTDGARARAMLDALRSRRPARVAGGLYNGLEAAAASAHAEIG
                     RMKAGLLAAGALGTALSGSGSTVFGIARSFEHARQIRARLVRGSWECWAVRTTCGRVR
                     "
BASE COUNT          327 a          774 c          740 g          336 t
ORIGIN      
        1 tggccgcggt cgttgaggat gcgcacgccc tcgggcacgg caaggtcgcc gacggtgagc
       61 acgtgaccga tcatgagtgg gctgacgtcg gcatcgatgc gctccgggat ccgggtcggc
      121 aggcaggaga cctcgagctc gtggagcacg atctgcagga ttcccttctg ctccttgacg
      181 ccggccgcct cgccgacggg atgcacggcg acccggacgg tgatggcccg atcggcctgc
      241 acctcctgca ggtccacgtg cagcaggctg tccgacaccg ggtcgaactg gaggtcgcgg
      301 atgatggcca tccgcgagcc gtcgtccccc tcgatcgcca gcgtcaggag ctgggtcgag
      361 cccgcgtggc cgtggaggat ccgaagcacc gcgcgcgggt cgacggtgac cgcctccggc
      421 ttgatgccgc cgtagagcac cccgggtacc gcgccggcgc gccgcagccg cttggcggcc
      481 tgcttgccgg tggcctcgcg gcgcctgatc gtcagttcct gcatcggcat gggtgacact
      541 ccttcatacg aacagggtcg agacggattc ctcgtcgtgg atccgccgga tggcttcccc
      601 gagcagcggc gccacggtga gcaccgtgat gcggcctgct gcacgcttgc cgtccgcgat
      661 cgggatcgag ttcgtgacga tgacctcctc gatgggggag gccttgatgc ggtcgatggc
      721 ggggcccgac agcaccgcgt ggatgccgca agcgatgatc cgtctggcgc cctctcgctg
      781 gatggcgccg accgcctgga tcagggtgcc ggcggtgtcg atcatgtcgt cgatgacgac
      841 ggcatcacga ccctcgacgt ccccgatgag gtgcatggcg acggcggagt tcggcccctc
      901 gcgccgcttg tcgatgatgg caagacccgc gctgaaccgc ttggcgatcg cgcgcgcgcg
      961 ctccacccct ccggcgtcgg gcgagatgat gacggggtcc cggagatcct tcccgcgcag
     1021 atagtcgatc atcaccaccg gacccgcgaa gaggtggtcc accgggatgt tgaagaagcc
     1081 ctggatctgc ccggcgtgca gatcgagcgc gagcacgcgg tccacgccgg ccgcggtgag
     1141 gaggtcggcc accagcttgg ccgtgatggg cacccggggc tgcaccttgc ggtcctgccg
     1201 cgcgtacccg tagtacggca gcacggcggt gatgcgtcgg gccgacgcgc gcttgaacgc
     1261 gtcgatcatg accagcagct ccatgagatt gtcgttcacc ggcggacacg tcggctggat
     1321 cacgaacacg tcggttcccc ggacgttctc gttgacctgc acgaacacct cgccgtcgga
     1381 gaaacgcgag acctccacgt cgccgagcgg caacttgagg tactcggcga tctcgtcggc
     1441 caggacccgg tgcgcgttgc ccgagaagag cttgaggtcg taggtcatcg tccgcctcgc
     1501 ctgacgttgg ttggggcggg aggattcgaa cctccgaata cgggatccaa agtcccgcgc
     1561 cttaccgctt ggccacgccc caccatacgc tccgatcttc gagccacttc accgtccggc
     1621 tcgccgcccg agcgcggctc acctcacacg atccggccat ctcagtggtc cggctcgccg
     1681 cccgagcgcg gctcacctca cacggccaca cgtcgtgcgc accgcccagc actcccagga
     1741 tccacgtacc aggcgcgcgc ggatctggcg cgcgtgttcg aaggagcgcg cgatgccgaa
     1801 cacggtggac ccgctgcccg acagcgcagt gcccagggcg ccggcggcga gcagcccggc
     1861 cttcatacgc ccgatctcgg cgtgtgccga cgccgcggcg gcctcgagac cattgtaaag
     1921 gcctccagcc acccgcgcgg ggcgccggct ccggagcgca tcgagcatcg cccgggcgcg
     1981 cgcgccgtcc gtgtacatct ccggcgtgac ccgtccgtag acctcggcgg tgctcgaccc
     2041 gaatcgcggg ttcaccagca cgagggcgag cccgacgccg ggcagcggct ccaacttctc
     2101 tcctcgcccg gtcgcgagtg ccgtaccgcc ccggaggaag aacggcacgt ccatcccgag
     2161 cgtgaccgcg atctcgc
//
LOCUS       DATIAZ010000038         4961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_403964, whole genome shotgun sequence.
ACCESSION   DATIAZ010000038 DATIAZ010000000
VERSION     DATIAZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403964"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VLF19_01295"
     CDS             <1..487
                     /locus_tag="VLF19_01295"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSE91911.1"
                     /translation="WIRVREDVAEMPDGRTTLYGVIECREAVGVLPFLDGEQVVLVGQ
                     YRYVARDFRWEMPTGAVHAGEDLEAAVRRELREEAGYEAGRLTRLCAFHTSKSVLDET
                     AHLYLAEGLRPASAEPDPTEFIEVRAFPFDEVLRMVETSEITDSMTVLAVLHAARRRE
                     R"
     gene            complement(604..1389)
                     /locus_tag="VLF19_01300"
     CDS             complement(604..1389)
                     /locus_tag="VLF19_01300"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSE91912.1"
                     /translation="MNHEVMDAQAAAYALGALEGEELTAFESHLAQGCARCEVTLRES
                     AEALAALARTAPSAVPPAHVREALLLRAAASAPRPSPAELRRTWLRWTAATAAAMLVG
                     GFLAGGWVASRYEARLGQAARQMSALRDQLRREEAALRARVAQYESVVDLLRDPATRV
                     VVLRGAGPSPEAHGRVVWNEAAGGHVLVARLAPTPEGKAYEAWTITGGRPSPGGVFRV
                     DASGHAVHRVAPPGGPVDVFAVTIEPESGTPTPTGPIVLVSAK"
     gene            complement(1386..1946)
                     /locus_tag="VLF19_01305"
     CDS             complement(1386..1946)
                     /locus_tag="VLF19_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSE91913.1"
                     /translation="MSPGVAGAELVRRMATGDRDAFGQFYDRYAPLVYPLIVRIVRTR
                     GDADDVLQEVFWEAWQAASAYDPNRGTPEAWIVMRARARAIDRIRSVRRRSDTFVAPI
                     DEALAPAPAEPGGDTADRTADRSLVTAALARLPENQREVIELAYYGGLTQTEIADRLK
                     QPLGTVKTRMRIGLERLRETVGRQAG"
     gene            2216..3826
                     /locus_tag="VLF19_01310"
     CDS             2216..3826
                     /locus_tag="VLF19_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91914.1"
                     /translation="MRVSAIAVAFAMLLTLPAAAQQSAEKLGTVNFQTSCNPAAQAEF
                     SRAVALLHSFWFQHAIRGFEAAAVADPSCGIAAWGAAVSWLGNPLAGAPPAKYVELGA
                     ATVARAKSMGAKTQRELDYIAAIETFYKDHDKVDHRTRALAYEKAMGALAARYPEDRE
                     AAIFYALALNITLNPNDKTYANQLKAAAILEKAFKDQPNHPGVAHYMIHSYDFPPIAG
                     KGLGAARRYAKIAPSAPHAQHMPSHIFTRLGYWQESIDTNRASVEAAKDELRQARLEA
                     GSYNALHAMDYITYAALQLVKDREARAVYDEIRRIEKIDTEHFAAAFAFTAIPARYTL
                     ERRQWAEAAELALHPQSLSWQKFPQAESILVFARGLGAARGGNVAGAKGEVARLEALR
                     DRMAAARNTYWAEQSEIQRLAVSGWIARAEKRNDEALKLLRAASDLEGGTEKHPVTPG
                     AIQPAREMLGELLLELGQPTQALAEFEASQRTDPNRFHGLAGAARAAELAGDRDKARG
                     YYQQLLALAKDADSERPELKQAKAYLAK"
     gene            4269..>4961
                     /locus_tag="VLF19_01315"
     CDS             4269..>4961
                     /locus_tag="VLF19_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE91915.1"
                     /translation="MRVPGAGMSGYGSPAKYEGKIHRTGIASQPGTTGSGASRTPLER
                     LEGMITPSGLHFERHHSGVPDIDPSAHRLLVHGLVKRPLVFTMDALLRYPMVSRIQFI
                     ECSGNSRPNLSPAPPAASCGAIHGLVSCSEWTGVPLAILLAEAGADRSAQWLLAEGAD
                     AAAMSRSVPVAKAMDDALLALYQNGERLRPENGYPLRLFLPGYEGNMSVKWLRRIKLT
                     AGPTMTKDETSKY"
BASE COUNT          748 a         1790 c         1745 g          678 t
ORIGIN      
        1 gtggatccgc gtgcgcgagg acgtggccga gatgcccgac ggccgcacga cgctctacgg
       61 cgtgatcgag tgccgcgagg cggtcggcgt gctgcccttc ctcgacggcg agcaggtcgt
      121 gctcgtcggc cagtaccggt atgtggcgcg cgacttccgg tgggagatgc cgacgggcgc
      181 cgtgcacgcg ggcgaggacc tcgaggccgc ggtccgccgc gagctacgcg aggaggcggg
      241 ctacgaggcc gggcgcctca cccggctctg cgcgttccac acctccaaga gcgtcctcga
      301 cgagaccgcg cacctctacc tcgcggaagg gctgcggccc gcgtccgccg agcccgatcc
      361 caccgagttc atcgaggtgc gtgcctttcc cttcgacgag gtgctgcgaa tggtggagac
      421 gagtgagatc accgactcga tgaccgtcct ggccgtgctc cacgccgcac gccggcgcga
      481 gcgctgaggc cccggagccc gtcggcgtgt cgaggcgggg ccgccgaccc aggccgcggg
      541 ccgcagcgag cgcggcggcc ccgccgggcg cgtcgccggg ccgaggacgc gccgagtgcc
      601 cgcctacttc gccgagacca gcacgatcgg cccggtgggc gtcggcgtgc cggactcggg
      661 ctcgatcgtg acggcgaaga cgtcgaccgg gccgccgggc ggggccacgc ggtgcacggc
      721 gtgcccggaa gcatcgacgc ggaagacccc cccggggctc ggccggccgc cggtgatggt
      781 ccacgcctcg taggccttgc cctcgggcgt cggggccagc ctggcgacca ggacgtggcc
      841 gccggccgcc tcgttccaga ccacccggcc gtgagcctcg ggactcggcc cggcgccccg
      901 caggacgacg acccgtgtgg cggggtcgcg tagcaggtca acgacgctct cgtactgggc
      961 cacgcgcgcg cgcagcgcgg cctcctcgcg acggagctgg tcgcgcagcg cgctcatctg
     1021 gcgcgcggcc tggccgagcc gtgcctcgta ccgcgaggcg acccatccgc cggcgaggaa
     1081 gccgccgacg agcatcgcgg ccgcggtcgc cgccgtccag cgaagccacg tgcgccggag
     1141 ctccgccggc gagggccggg gcgcggaggc cgccgcccgc agcaggagcg cctccctgac
     1201 gtgagccggc ggcacggccg acggcgccgt gcgcgccagc gccgccagcg cctcggccga
     1261 ctcgcgcagc gtcacctcgc agcgcgcgca gccctgcgcc aggtgcgact cgaaggccgt
     1321 cagctcctcg ccctcgagcg cgccgagcgc gtacgccgcc gcctgggcgt ccatgacctc
     1381 gtgattcatc ccgcttgccg tcccacggtc tcccgcagcc gctccagccc gatccgcatg
     1441 cgggtcttca ccgtgccgag cggctgcttc aggcgatcgg cgatctcggt ctgcgtcagc
     1501 ccgccgtagt acgcgagctc gatcacctcg cgctggttct cgggcaaccg cgcgagcgcc
     1561 gctgtcacga ggctgcgatc ggccgtccgg tcggcggtgt cgccgcccgg ctccgccgga
     1621 gccggagcga gtgcctcgtc gatcggcgcc acgaacgtgt cgcttctcct acgaacggat
     1681 cggatccggt cgattgccct ggcccgggcc cgcatgacga tccaggcctc gggcgtaccg
     1741 cggtttggat cgtaggccga ggctgcctgc cacgcctccc agaaaacctc ctgcagcacg
     1801 tcgtcggcgt ccccacgcgt acgaacgatc ctcacgatga gggggtaaac gaggggggca
     1861 taccggtcgt agaactggcc gaaggcgtcc cgatcgccgg tggccatccg ccgcacgagc
     1921 tccgcgcccg cgaccccggg tgacatgacg cgccactcac tagcagacag gttcggcatt
     1981 gacaagctta ccgagggaat acgagcgatc ctcggacgct ggatcgcgtc gccgcttgtc
     2041 gtcggcggca ggctttcggg catgtcgccc gccttcgggc aatccgcccg tcttctcgcc
     2101 acgttgcctc tctgcaagac cgcgtcatgg cggacattgg gcccggcacg tcttgtgccg
     2161 ggtgtgaggg cattcagcga agctccctgc cttcgggtgg ggaaggagga cgacgatgcg
     2221 cgtttcagca atcgccgtgg cattcgctat gctcctcacc cttcccgcgg cggcccagca
     2281 atccgccgag aagctgggga ccgtgaactt ccagacgtcg tgcaacccgg ccgctcaggc
     2341 ggagttctcc agggccgtcg ccctgctgca ttccttctgg ttccagcacg ccatcagagg
     2401 gttcgaggcc gcggcggtgg ccgatccgtc ctgcggcatc gcagcctggg gggcggcggt
     2461 ctcgtggctg ggcaacccgc tcgccggagc accaccggcc aagtacgtcg agctgggcgc
     2521 cgccaccgtg gcccgcgcca agagcatggg cgccaagacc cagcgtgagc tcgactacat
     2581 cgcggccatc gagaccttct acaaggacca cgacaaggtc gaccatcgca cgcgcgcgct
     2641 cgcctacgag aaggccatgg gggcgctggc cgcccgctat cccgaggacc gcgaggcggc
     2701 catcttctac gcgctcgcgc tcaacatcac tctcaatccg aacgacaaga cctacgcgaa
     2761 ccagctcaag gcggcggcca tcctcgagaa ggcgttcaag gaccagccca accatccggg
     2821 cgtggcccac tacatgatcc acagctacga cttcccgccc atcgccggca agggactcgg
     2881 cgccgcgcgc cggtatgcca agatcgcgcc gtcggcgccg cacgcgcagc acatgccatc
     2941 ccacatcttc actcggctcg gctactggca ggagtcgatc gacaccaacc gggcctcggt
     3001 cgaagccgcc aaggacgagc tgcgccaggc gcggctggag gcgggctcgt acaacgcgct
     3061 gcacgccatg gactacatca cgtacgccgc gctgcagctc gtgaaggacc gggaagcgag
     3121 ggccgtgtac gacgagatcc gccgcatcga gaagatcgac accgagcact ttgccgcggc
     3181 gttcgccttc accgccatcc cggcgcgcta cacgctggag cgtcggcagt gggcggaggc
     3241 ggcggagctg gcgctccatc cgcagagcct gtcgtggcag aagttcccgc aggcggagtc
     3301 catcctcgtg ttcgcacgcg gcctgggcgc agcacgcggc ggcaatgtcg ccggcgccaa
     3361 gggcgaggtc gcccgcctgg aggcgctccg tgaccgcatg gccgcagcgc ggaacacgta
     3421 ctgggcggag cagtcggaga tccagcggct ggcggtgtcc ggctggatcg cccgggccga
     3481 gaagcgtaat gacgaggcgc tgaagctcct gcgcgcggcg tccgacctcg agggtgggac
     3541 ggaaaagcac ccggtcaccc cgggcgcgat ccagccggcc cgcgagatgc tcggtgagct
     3601 gctgctcgag ctggggcagc ccacccaagc gctggcggag ttcgaggcct ctcagcgcac
     3661 ggacccgaat cggttccacg gcctcgcggg agcggcgcgc gccgccgagc tggccggcga
     3721 ccgcgacaag gcgcgcggct actaccagca gctgctcgcc ctggcgaagg acgccgactc
     3781 cgagcgcccg gagctgaagc aggcgaaggc gtacctcgcg aagtagcggg cgcaacacca
     3841 gggccgccga gccgaaccgg ctcggcaagc cgccgtgctc cgcggccctg ggtagccccg
     3901 tcagccccgg tgatggacgc agccgggtaa cccggtcctg gtcgcgctgt cgcccgggct
     3961 cagccgccag catgctcggg ttgcgccgta cggcgccgct tgcggggggc gcgaccatcg
     4021 cggtagagtc caagtcactc gagtggcgcc gtttcgaccg tcgtgtcagc acggattcgt
     4081 gacgggagga gacacacatg ccgagctcga gcccgccgag agtctcgctc gaagaagtcg
     4141 ccgggaacgg attgctgcat cgccggatct tcctgaccca gggcgtcgct ctcatcggtg
     4201 caggaggcct cacggtgctg tcggcggggt cggcggtggc cgcggaaccg cccgacatac
     4261 caccctggat gcgcgttcca ggggcgggga tgagcggcta cgggtccccg gcgaagtacg
     4321 agggcaagat ccatcgaacc ggaatcgcct ctcagcccgg cacgacgggt agcggcgcat
     4381 cccgcacgcc gctcgagcga ctcgagggca tgatcacgcc gagcggtttg cacttcgagc
     4441 gccatcacag cggcgtgccg gacatcgatc cgagcgctca ccgtctgctc gttcacggtc
     4501 tggtcaagcg gcccctggtg ttcacgatgg atgccctcct gcgctatccg atggtgtcgc
     4561 gcatccagtt catcgagtgc tcgggaaata gccgaccgaa cctgagtcct gcgccccccg
     4621 cggcgtcctg tggagcgatc cacggcctcg tctcgtgcag cgagtggacg ggggtgccgc
     4681 tggcgatctt gctggcggag gccggagcgg accgtagcgc gcagtggctc ctcgccgagg
     4741 gcgccgacgc ggcggcgatg agccggagcg tgccagtggc caaggcgatg gacgacgcgc
     4801 tgctcgcgct ctaccagaac ggtgagcgcc tccggcccga gaacggctat ccgctgcgcc
     4861 tgttcctgcc gggctacgag ggcaacatga gcgtcaagtg gttgcggcgg atcaagctca
     4921 ccgccgggcc gacgatgacc aaggacgaga cctcgaagta c
//
LOCUS       DATIAZ010000039         7165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_272548, whole genome shotgun sequence.
ACCESSION   DATIAZ010000039 DATIAZ010000000
VERSION     DATIAZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272548"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1250)
                     /locus_tag="VLF19_01320"
     CDS             complement(243..1250)
                     /locus_tag="VLF19_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91916.1"
                     /translation="MATLVGVVSTTHSPTCYLPAERWNEVRASRALRADVPMDDLEEN
                     RRKKARVEQGFATLKQKLMAAAPDVLVVFGDDQLECFDFTNFPSFAVYVGETFEGALS
                     TGERVKLPGHHDLAVGLVGGLMRRGFDPAFCMDMPKPERGVGHAFLRPAESLTDFRTP
                     IVPFFVNCYYAPQPTGMRCYQLGRAVHEIIAEHPGDLRVAVVGSGGLWHTPRATDAYL
                     DEDFDRAMLRYLTAGDARGAARHFDEYKVPAGDRSQDVGTRGRSTTGMPGPGGPQGGT
                     RETCNWIIAAAVVDGKPATLVDYVPVYASPIGAAFAYWPDVQAARRSSFAPGFRSRRD
                     R"
     gene            1357..2667
                     /locus_tag="VLF19_01325"
     CDS             1357..2667
                     /locus_tag="VLF19_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSE91917.1"
                     /translation="MLSREDNELLCRIGPGTPMGALMRQYWVPAALSSELPETDGPPL
                     RVRLLGEDLIAFRVSSGAVGLIQNACPHRGASLFYGRNEAEGLRCVYHGWKFDVTGCC
                     VDMPSEPAESSFKHKVKAVAYPCVERAGLIWAYLGPRQTPPAMPDLEPIVGEGSTIQI
                     YQRECNWMQALEGDIDTGHTVFLHLGGVAEGDVPPGTWSRYALSKRAPRYEVVETDFG
                     VMYGACRPAEADTNYWRIANFLFPFYAMVPTGVLGLEVRVRAWVPMDDDHTLAITISR
                     GQPPQSRNAGRQVVGPPETLPNTTDWYGRFRCVADAGNDYLIDRKSQKAASYTGIGSI
                     FLQDQAVTESMGPIYNRTQEHLATSDTMVIRTRKRLIDAARALRDQGQVPPGVDAPAV
                     YRVRSGGVVLPRDADWIEATKELRRAWAEHPGLSRSVLGGLPAV"
     gene            2669..3499
                     /locus_tag="VLF19_01330"
     CDS             2669..3499
                     /locus_tag="VLF19_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE91918.1"
                     /translation="MASLVSTLPELVEYGLRGSVPPRRRVLGVKVAAVLGLLAVWGLI
                     AGLVAALRLFNPIFFPGPWVVVGALVELGVKGQLWPHVVTTLERVVVGFAAGAGLALA
                     LGLLAGAVRPVRNLIEPVVELLRPIPPLAMLPLFIVWVGIGEASKVGFITYATFFPMF
                     LTTVHAVGQIDPVLVRAARSLGARRLQLFRRVILPAALPDILTGLRLGVALSFFVIVI
                     SEFIGAEHGLGYLINDGRNFFLVAQMLGAAVVLGLLGWAGNGLVRLAERRLLRWQHPS
                     "
     gene            3508..4269
                     /locus_tag="VLF19_01335"
     CDS             3508..4269
                     /locus_tag="VLF19_01335"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE91919.1"
                     /translation="MKIRIERLGKSFGTDARRSAALTGIDLDVADREFVCILGPSGCG
                     KSTLLNIVAGFLPPSDGRVLVDGRPVSGPGADRGVVFQEYALFPWLTVADNVDFGLVV
                     RGQPAADRRRIVGRYLDLVGLAAHAGKFPAQLSGGMKQRVAIARALATNPAIILMDEP
                     FGALDAQTREVLQDELSRLQRLEHKTVLFVTHSIREAVYLADRVVVMTSAPGRIKEVF
                     TIKLPEARDRFAPEFTRYESEITRIVKEEVAKVRE"
     gene            4266..5027
                     /locus_tag="VLF19_01340"
     CDS             4266..5027
                     /locus_tag="VLF19_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE91920.1"
                     /translation="MRTTLTRAGAMVAVALVWALVAHANRAREFVNPVLLPTPAEVLA
                     VAIDTVRDGTLHRHVLTSLGRVAQGFGLAAAAAVALGVLAGVSLPLRLVADPIVEFVR
                     PIPPLAFLPMFLVWFGLGEASKVAFIGYTTFFPMFVAVAASVVRVDVVLLRAAASLGA
                     SRADLIRRVILPAALPGIVVALRLGVGLALFVIVGAEFMGADAGLGHLIMEGRTFFNP
                     AQIVMGALVLGLLGSLVNALLLAIERRMLRGRAAM"
     gene            5302..6336
                     /locus_tag="VLF19_01345"
     CDS             5302..6336
                     /locus_tag="VLF19_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE91921.1"
                     /translation="MHASVVALTLLTILVGASPALAQGKPEVAEVEAWLVRDAQMSSQ
                     FAVADQLGYFKAEGIKVVPRWYIAGTDLPSMWGAGNIHLGTATATMVVPIAASGQAIY
                     NIAPQSDIAGTQQIILGKKGQEIVRSPKDFEKLKIGMPKGASVTMAIQAMARETGVDF
                     TKIQFVNLSPPDAVTALAKGDIDAMAAWAPWVFNAVKQAGGKFYFSGNKSAIPGKEGA
                     VDWLHVHAGVVASGKMLKENPNTLKAVLRALRKATDTLNKDRETAVKIVAREMKTDEA
                     LTRDIMALNVYSMEVDDKHVRGMGEFVDFLHSLDRIKQKFPPESVFYTRLLEEVDPKL
                     VKWKSKTEVR"
     gene            6336..>7165
                     /locus_tag="VLF19_01350"
     CDS             6336..>7165
                     /locus_tag="VLF19_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE91922.1"
                     /translation="MVRVHLRVPGTAPMPALMRLLQDIEAAGFDGAGILDSQLLCRDT
                     FVTLGQAATHTSRLALFPAVTNPFTRHASVLAGAIQTVEELAPGRVRFVIGTGYTSAS
                     TIGRPPGTLAEMRACIGTVKTLLAGESVDFGGTPGRLAFASRRRIPVLMAASGPKAIE
                     VAGEIADGALLLVGFNRGIVETALDHLERGVKRAGRRLDDLEIIWAVRTGTAATTEEA
                     RRLARPTAVHWGILRWGGHWLGPAGLRLPKLEIPDAVHKIYPDLSHARDWEAAIAATS
                     "
BASE COUNT         1012 a         2524 c         2529 g         1100 t
ORIGIN      
        1 gagcgcgttg gagttgggat cccatcccgg cggatggatg acggccgcgt tcacgccgcc
       61 ctcgtccatc tccttcagca cgtcggcggc cgtgaagtca ctgatctgac gatggttcgg
      121 gttcgtcggc ttgtgtcctg cccagatgtg gatctgagcg tcgacgatca gcatggggtg
      181 gtccctacct ttctatccgc gcaggggcgg agaccttcgc gggcgccagc cgcgtaaccg
      241 tatcatcgat cccttcgcga ccggaaccca ggcgcgaacg aggatctgcg cgccgcttgc
      301 acgtccggcc agtacgcgaa cgctgcgccg atgggcgagg cgtagacggg cacgtagtcg
      361 acgagggtgg cgggcttgcc gtcgacgacg gcggccgcga tgatccagtt gcaggtctcg
      421 cgcgtgccgc cctgcggccc gccgggtccg ggcatgccgg tcgtgctcct tccgcgcgtg
      481 ccgacgtcct gactgcggtc gccggcgggc accttgtatt cgtcgaagtg gcgcgccgcc
      541 cccctcgcgt ctcctgccgt caggtagcgc agcatcgccc ggtcgaagtc ctcgtcgagg
      601 taggcgtcgg tggcgcgggg cgtgtgccag agtccgccgg agccgaccac ggccacgcgt
      661 agatcgcccg gatgctcggc gatgatctcg tgcacggcgc ggccgagctg gtagcagcgc
      721 atccccgtcg gctgcggggc gtagtagcag ttgacgaaga acggcacgat cggcgtgcgg
      781 aagtccgtca gcgactcggc gggccggagg aacgcgtggc cgacgccgcg ctcgggcttg
      841 ggcatgtcca tgcagaacgc ggggtcgaag ccccggcgca tgagcccgcc gaccagcccg
      901 acggccaggt cgtggtgacc cggcagcttc acccgctcgc ccgtcgagag cgcgccctcg
      961 aacgtctcgc ccacgtacac cgcgaacgac gggaagttcg tgaagtcgaa gcactcgagc
     1021 tggtcgtcgc cgaaaacgac gaggacgtcg ggggcggccg ccatcagctt ctgcttcagc
     1081 gtggcgaagc cctgctcgac gcgcgccttc ttccggcggt tttcctcgag gtcgtccatc
     1141 ggcacgtcgg cccgcagcgc gcggctggcg cgcacctcgt tccagcgctc cgccggcaga
     1201 tagcaggtgg ggctgtgggt ggtgctgacg acgccgacca gggtggccat cgctacgctc
     1261 cgcggtgaga atctaccatc attgcgccgg tatgataccg tctggcggtc ggccggctga
     1321 tggccctcgg atcgcgggcc cgggagggaa cacgccatgc tgagtcgaga ggacaacgag
     1381 ctgctctgtc ggatcgggcc ggggaccccc atgggcgcgc tcatgcgcca gtactgggtg
     1441 ccggcggcgc tgtcgagcga gctgccggag accgacggcc ctccgctgcg cgtgcgcctg
     1501 ctgggcgagg acctcatcgc cttccgcgtc tcgtccggcg ccgtcggact gatccagaac
     1561 gcctgcccgc accgcggcgc gagtctcttc tacgggcgga acgaagcgga aggcctgcgg
     1621 tgcgtgtacc acggctggaa gttcgacgtg acgggctgct gcgtggacat gccgagcgag
     1681 cccgccgaga gcagcttcaa gcacaaggtg aaggccgtcg cctatccctg cgtggagcgc
     1741 gccggcctga tctgggccta cctcgggccg cgtcagacgc cgccggcgat gccggacctg
     1801 gagcccatcg tgggcgaggg cagcaccatc cagatctacc agcgcgagtg caactggatg
     1861 caggcgctgg agggcgacat cgacaccggc cacaccgtgt tcctgcacct tggcggcgtc
     1921 gccgaggggg acgtgccgcc cggcacctgg tcccggtacg cgctgagcaa gcgcgcgccg
     1981 cgctacgagg tggtggagac ggacttcggc gtcatgtacg gcgcctgtcg gcccgccgag
     2041 gcggacacga actactggcg catcgccaac ttcctgttcc cgttctacgc gatggtgccg
     2101 accggcgtgc tgggactcga ggtccgcgtg cgcgcctggg tgcccatgga cgacgaccac
     2161 acgctggcca tcacgatcag ccggggccag ccgccacaga gccggaatgc cgggcgccaa
     2221 gtggtgggcc cgcccgagac gctgccgaac accaccgact ggtacggccg gttccggtgc
     2281 gtggccgacg cgggcaacga ctacctcatc gaccggaaat cgcagaaggc cgccagctac
     2341 acgggcatcg gcagcatctt cctccaggac caggcggtca ccgagagcat ggggccgatc
     2401 tacaaccgca cccaggagca cctggcgacc agcgacacga tggtcatccg gacccgcaag
     2461 cgactgatcg acgccgccag ggcgcttcgc gaccaggggc aggtgccgcc gggagtggac
     2521 gcccccgcgg tctaccgcgt acggtcgggc ggcgtcgtcc tgccgcgcga cgccgactgg
     2581 atcgaggcga cgaaggagct gcgccgcgcg tgggccgagc acccggggct cagccgctcc
     2641 gtgctcgggg gtctgcccgc cgtctgacgt ggcctcgctc gtcagcacgc tccccgagct
     2701 ggtggagtac gggcttcgcg gatccgtgcc gccgcgtcgc cgcgtcctgg gtgttaaggt
     2761 ggccgcggtg ctcggcctgc tcgccgtatg gggcctcatc gccggactcg tcgcggcgct
     2821 gcgcctcttc aacccgatct tcttcccggg cccgtgggtc gtcgtcggcg ccctcgtcga
     2881 gctgggcgtc aagggccagc tctggcctca cgtcgtgacg acgctcgagc gggtcgtcgt
     2941 cgggttcgcc gccggcgccg gcctcgccct cgcgctcggt ctgctcgcgg gcgccgtccg
     3001 gcccgtgcgc aacctgatcg agccggtcgt cgagctgcta cggccgatcc cgccgctggc
     3061 catgctgccg ctcttcatcg tctgggtggg tatcggcgag gcctcgaagg tcgggttcat
     3121 cacctacgcc acgttctttc ccatgttcct gacgacggtg cacgccgtcg gacagatcga
     3181 ccccgtgctg gttcgcgcgg cccggagcct gggagcgcgg cggctgcagc tgttccgccg
     3241 ggtgatcctg ccggcggcgc tgcccgacat cttgacgggg ctgcgcctcg gcgtggccct
     3301 gtccttcttc gtcatcgtga tctccgagtt catcggcgcc gagcacgggc tcggctacct
     3361 gatcaacgac gggcgcaact tcttcctggt cgcccagatg ctcggcgccg ccgtcgtcct
     3421 cgggttgctc ggctgggcgg gcaacggcct cgtgcgtctc gccgagcgtc ggctcttgcg
     3481 ctggcagcat ccgtcatgaa cggcagcgtg aagattcgca tcgagcgcct gggcaagtcg
     3541 ttcggcaccg atgcgcgccg gtcggcggcg ctgaccggca tcgatctcga cgtggccgac
     3601 cgcgagttcg tctgcatcct cggcccgtcg ggatgcggca agtcgacgct cctcaacatc
     3661 gtcgccggct tcctgccgcc gagcgacggg cgcgtgctgg tggacggccg tccggtcagc
     3721 gggcccggcg ccgaccgggg tgtggtcttc caggagtacg cgctcttccc gtggctcacg
     3781 gtggccgaca acgtggactt cggtctcgtg gtgcgcggcc agccggccgc cgaccggcgg
     3841 cggatcgtcg gccgctacct cgacctggtg ggactcgccg cgcacgccgg caagttcccg
     3901 gcccagctgt cgggcggcat gaagcagcgc gtggccatcg cccgcgcgct ggccaccaac
     3961 ccggcgatca tcctgatgga cgagccgttc ggggccctcg acgcccagac ccgcgaggtc
     4021 ctgcaggacg agctgtcgcg gctgcagcgt ctggagcaca agaccgtcct gttcgtcacg
     4081 cactcgatcc gcgaggcggt gtacctcgcc gaccgcgtgg tcgtcatgac gtcggcgccc
     4141 ggccgcatca aggaggtgtt cacgatcaag ctgcccgagg cgcgcgaccg cttcgcgccc
     4201 gagttcaccc gctacgagag cgagatcacg cggatcgtca aggaagaagt ggccaaggtg
     4261 cgcgagtgag gacgaccctg acccgcgccg gggcgatggt ggcggtcgcg ctcgtgtggg
     4321 cgctcgtcgc ccacgccaac cgcgcgcgcg agttcgtgaa cccggtgctg ctacccaccc
     4381 cggcggaggt gctggcggtg gcgatcgaca cggtccgcga cggcacgctg caccgccacg
     4441 tcctcacgtc gctggggcgg gtcgcccagg gcttcgggct ggcggcggcc gccgcggtgg
     4501 cgctgggagt gctggccggc gtctcgctgc cgctccggct cgtcgcggat ccgatcgtgg
     4561 agttcgtccg ccccatcccg ccgctggcct tcctgcccat gttcctggta tggttcggcc
     4621 tcggcgaggc ctcgaaggtg gccttcatcg gctacacgac gttctttccg atgttcgtgg
     4681 ccgtcgccgc cagtgtcgtg cgggtggacg tggtgttgct gcgcgcggcg gccagtctcg
     4741 gcgcctcccg cgccgatctc atccgccgcg tgatcctgcc ggccgcgctg ccgggcatcg
     4801 tcgtcgcgct gaggctcggc gtgggcctgg cgctgttcgt gatcgttggc gcggagttca
     4861 tgggcgccga cgccgggctc ggacacctga tcatggaggg ccgcacgttc ttcaacccgg
     4921 cccagatcgt catgggagcc ctcgtcctcg ggctgctcgg ctcgctcgtc aacgcgctgc
     4981 tgctcgccat cgagcgacgc atgctccgcg gccgggcggc gatgtgagag cctgtcggga
     5041 ccgaccgaca tgggggcccc gacatggccc ccaagccccc aaggcggctc ggcacgcccc
     5101 ggcaaggccg tgacgcgcct cgatctcgcg cgagggccag gggggccccg gacatggccc
     5161 ccaagccccc aaggcggctc ggcacccccc ggcaaggctg tggcgcgcct cgatctcgcg
     5221 cgagggccag gggggccccg acatggcccc ccaggccgcc aacgttacga aacgccccgt
     5281 gccgctcgaa aggagtcgtc catgcatgca tccgtcgtcg cgttgaccct gctcacgatc
     5341 ctggtcgggg cctcgccggc gctcgcccag ggcaagcccg aggtcgcgga ggtcgaggcg
     5401 tggctcgtcc gcgatgccca gatgtcgtcc cagttcgccg tcgccgatca gctgggttac
     5461 ttcaaggccg agggcatcaa ggtcgtcccg cgctggtaca tcgccggtac cgacctgccc
     5521 tcgatgtggg gcgccggcaa catccacctc ggcacggcca ccgccaccat ggtcgtgccc
     5581 atcgcggcgt ccgggcaggc gatctacaac atcgctccgc agagcgacat cgccggcacc
     5641 cagcagatca tcctcggcaa gaaggggcag gaaatcgtcc gctcgcccaa ggacttcgag
     5701 aagctcaaga tcggcatgcc caagggcgcc tcggtcacga tggccatcca ggcgatggcc
     5761 cgcgagaccg gcgtggactt caccaagatc cagttcgtga acctctcacc ccccgacgcg
     5821 gtgacggcgc tggcgaaggg cgacatcgac gcgatggccg cctgggcgcc ctgggtattc
     5881 aacgccgtca agcaggctgg cggcaagttc tacttcagcg ggaacaagag cgcgataccg
     5941 ggcaaggagg gcgccgtcga ctggctccac gtgcacgcgg gcgtggtggc cagcggcaag
     6001 atgctgaagg agaaccccaa cacgctgaag gccgtgctcc gggccctgcg caaggcgacc
     6061 gacacgctga acaaggaccg cgagaccgcg gtgaagatcg tcgcacgcga gatgaagacg
     6121 gacgaggccc tgacgcggga catcatggcc ctcaacgtgt actcgatgga ggtcgacgac
     6181 aagcacgtgc gcgggatggg cgagttcgtc gacttcctgc actcgctcga ccggatcaag
     6241 cagaagttcc cgccggagag cgtcttctac acccggctgc tggaggaggt cgatcccaag
     6301 ctggtgaagt ggaagtccaa gaccgaggtg cgctgatggt ccgcgtccac ctgcgggtgc
     6361 ccggcaccgc gccgatgccc gcgctcatgc ggctgctcca ggacatcgag gcggcgggct
     6421 tcgacggcgc cgggatcctc gacagccagc tcctgtgccg cgacaccttc gtcaccctcg
     6481 gccaggccgc cacccacacc tcgcgcctgg cactcttccc cgcggtgaca aacccgttca
     6541 cccgtcacgc ctcggtactc gccggcgcca tccagaccgt cgaggagctg gcgccggggc
     6601 gagtgaggtt cgtcatcggt acgggctaca cgtcggccag cacgatcggc aggccgcccg
     6661 gcacgctggc cgagatgcgc gcctgcatcg gcacggtgaa gacgctgctg gcgggggaga
     6721 gcgtcgactt cggcgggacg cccggccggc tggcattcgc gtcgaggcgg cggatcccgg
     6781 tgctgatggc cgcctcggga ccgaaagcca tcgaggtcgc gggagagatc gccgacgggg
     6841 cgctcctcct cgtcggcttc aaccgcggca tcgtggagac ggcgctcgac cacctcgagc
     6901 gcggcgtcaa gcgggccgga cgccggctcg acgatctcga gatcatctgg gccgtgcgga
     6961 cgggcacggc ggcgacgacc gaggaggcgc gccgcctggc gcgccccacg gcggtccact
     7021 ggggaatcct gcgatggggc ggccactggc tggggccggc cggactgcgt ctgccaaagc
     7081 tcgagatccc ggacgcggtg cacaagatct atcccgatct gtcccacgcg cgcgactggg
     7141 aggcggccat cgcggccacc tcgtt
//
LOCUS       DATIAZ010000040         8163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_272593, whole genome shotgun sequence.
ACCESSION   DATIAZ010000040 DATIAZ010000000
VERSION     DATIAZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272593"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VLF19_01355"
     CDS             <1..1504
                     /locus_tag="VLF19_01355"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HSE91923.1"
                     /translation="TRGLTAVVGFIDRRDDVFNAAAVLHDGARAGVYHKQYLPNYGVF
                     DENRYFQAGAATPVFTVGDTTFAVNICEDVWYPAGPTTAQALAGAELVVTINGSPYHA
                     GKAHVRERMVATRAADDLVCLAFVNMVGGQDELVFDGGSFVVSERGEVVVRGRAFEED
                     LVVADLDLDAVFHARLHDPRRRQEKLRAGDGVTRVALPALPAPAVLPALAPREVPALS
                     PVAEVFQALVLGTRDYVRKSGFKHVVIGLSGGIDSSLVAAVAVEALGRENVTGVTMPS
                     PYSSGGTKSDARRLARNLGIELLRLPITPVFAAFKRALARPFEGLKEDVAEENIQARV
                     RGTLLMALSNKFGWLVLTTGNKSEIGVGYSTLYGDMAGGFAVIKDVPKTLVYELSRHL
                     NARAGRELIPESVFTRAPSAELRPDQTDQDTLPPYPVLDAILEAYVEDDRSVADLAAL
                     GFDPEIARKVVRMVDANEYKRRQGPIGVKITPRAFGRDWRLPIVNRFRQP"
     gene            complement(1591..3336)
                     /locus_tag="VLF19_01360"
     CDS             complement(1591..3336)
                     /locus_tag="VLF19_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE91924.1"
                     /translation="MRLLAPYRRLLPYIRPHTPTLVLGGALALVVAAMDGAIAWLVKP
                     VMDDIFIRRDAAMLKLVPPALLGAYLVKGAARYFQAYLMAAVGERVVARLRRDLYTHI
                     QSMPLAFFAGMHSADLMSRILNDVNRLARLASTVLVMAVRQVGTIVALLAVMLAREWL
                     LTLAALVAFPFIALIVRVIGRKLYAINRRFQERVAQLAVLLHEAFSGTKIVKAFGREA
                     HEQQRFDAINARLLDLSLRNVRADEITEPLMEVAGALGIVAAILYGGYRVIAGDMTPG
                     TFFSFTAAVIMLYGPARRLSRSLNTVQQTTASVERVFEIVDQPPAVADRAGAVALEEF
                     RDAIRFEAVAFRYARGTEPALRDISLTIRKGEVVAFVGMSGAGKSTLMDLLPRFHDVT
                     EGRITVDGHDVRDITQASLRALMGIVTQETFLFSDTIRYNIAYGQPDAPDEAIERAAR
                     QAQAHDFVVACPEGYQTLVGERGVRLSGGQRQRIAIARAFLKNPPILILDEATSDLDA
                     ESEFMIQQALADLMKGRTVLVIAHRLATVRNADRIVVVHEGRIAEVGRHGELLARDGL
                     YRRLHALQMTPAGTR"
     gene            complement(3333..4433)
                     /locus_tag="VLF19_01365"
     CDS             complement(3333..4433)
                     /locus_tag="VLF19_01365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSE91925.1"
                     /translation="MRILQLYPKSDYFTGAAIQLRELCLGLAERGHEVVLATRPSETW
                     AEKTRAAGLPHYALPMASEVDLQSVRRLVRIIRKHRIEVVHAQKGKARTLSLMAGLFA
                     RIPVLVLNRGVSFPLDPFARLGYNARRVTAVVAVCESIQRGLIASGVRADKIQVIYSG
                     TDTDRFHPGVDGSGIRRGLGLDPAAFLVTQIGVRSWKGNDDVVDAMGAVAARAPQAHL
                     LIVGARRPEVLLERARALGLEGRAHVWGYREDVPEILAASDCCVDASYTGLGLTGTVR
                     EALAVETAVVATAIEGNPELVIDGQTGLLVPPRDPAALAVAILRLIEDPALRRATARA
                     GRELVRARFSTRAKLDATETLYRRLLAERERA"
     gene            complement(4439..5518)
                     /locus_tag="VLF19_01370"
     CDS             complement(4439..5518)
                     /locus_tag="VLF19_01370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSE91926.1"
                     /translation="MTIPLSRPPVDDEIKQAVLAAMDSRQYILGPQCRELEAELARHA
                     GLRHAVLTTSATAALWMTMKALGVKPGDEVLTPAHTAFPTIEAICCAGGTPVFVDVDD
                     WHTMDPKDAQAKVTPRAVGILPVHLYGQPADLPAIQDVATRHGLWVLEDCAQAQGAAW
                     DARAVGGFGRAAVLSFYPSKNLPAMGDGGAVLTSDDEIAARCRRLRDHGRLSKDVHAE
                     LGFNLRFSELQAAALRVLLRRLPAMNERRRALAARYAAGLAGLPLGLPDEREHARHVY
                     HLYVVRAPERDGLAAFLKARGIQTGVHYPVPTHRQPAMATLTPPALPRTEQLVREILT
                     LPMSPNHTDAEIDTVIATVRAFFGR"
     gene            complement(5515..5997)
                     /locus_tag="VLF19_01375"
     CDS             complement(5515..5997)
                     /locus_tag="VLF19_01375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSE91927.1"
                     /translation="MIGPDDFRRVLGHFATGVTVVTTYDADQRPTGLTVSAFSSLSLD
                     PPLVLVCVDHKSQSYPALRDSGRFAVNVLATDQEAVSRRFATTRMDKFDGLPHRLGTL
                     GTPLIEGALVHLECVTVAAHVEGDHTVFVGRVEEAGGGEGEPLLYYRGKYGRLQGGPS
                     "
     gene            6093..7229
                     /locus_tag="VLF19_01380"
     CDS             6093..7229
                     /locus_tag="VLF19_01380"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSE91928.1"
                     /translation="MITERAVLDVLRTIKDPDGQQDIVALGLVRDLAIQDTEVSFTLA
                     FTTQAPATKAALHAAASRAVAQVPGVAKVTARIGAASRPAPGGHAGHAEQAGHAHHAG
                     QAPGAAGSAQAADLIPEVKHTVAVSSGKGGVGKSTVAVNLAIALRMAGAQVGMVDSDV
                     YGPDVPLMLGSRGKPGMFENKIIPVEAHGIKMMSIGLLVGDRESLVWRGPMIHSFIQQ
                     MLRDVMWGALDYLVFDMPPGTGDAQLSLSQVIPLSGVVMVTTPQDVALLDVRKAVGMF
                     QRLNVPILGIVENMSWFACPHCGERTEIFGDKGGRRLAEEYGVPLLAQLPLDPETRKG
                     GDEGVPITLRRPDAPQARAFRDLGAAVRRRLDELAPLRSLPKIG"
     gene            7247..7903
                     /locus_tag="VLF19_01385"
     CDS             7247..7903
                     /locus_tag="VLF19_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSE91929.1"
                     /translation="MSSPDPTPPAAALVLPVFPLPDVTLFPHVPLPLHIFEARYRAMV
                     SDALARDRRLCVARLEPGWEAGYNGKPPVAPIAGAGEIVSWERLATGRYNIVVRGQWR
                     VRIERELPTDTLYRVMRMRRLADEPAAADPAPRLGRIRAACLRLLDAVGRPSGLLDPL
                     LAPEQPPGVIADRVAAAVLPDARQRQELLETLDVGVRLERLAAALDALVEELRKGGKP
                     "
     gene            7900..>8163
                     /locus_tag="VLF19_01390"
     CDS             7900..>8163
                     /locus_tag="VLF19_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSE91930.1"
                     /translation="MTPAVRLLALVALLVAASAADARSWAWLGVRIRDMTEQEMEEIS
                     KRHGIREGFGVVIVEVLEGTPAARAGLKNGDIVVAFGERPVTET"
BASE COUNT         1145 a         2873 c         3031 g         1114 t
ORIGIN      
        1 cacgcgcggg ctcaccgcgg tcgtgggctt catcgaccgc cgggacgacg tcttcaacgc
       61 cgccgccgtc ctgcacgacg gcgcgcgggc cggcgtctac cacaagcagt acctgccgaa
      121 ctacggcgtc ttcgacgaga accgctactt ccaggcgggt gcggcgacgc cggtgttcac
      181 cgtgggcgac acgaccttcg ccgtgaacat ctgcgaggat gtctggtacc cggcggggcc
      241 gacgaccgcc caggcgctgg ccggggcgga gctggtggtg accatcaacg gctcgcccta
      301 ccacgccggc aaggcccacg tgcgcgagcg catggtggcg acgcgcgccg ccgacgacct
      361 cgtgtgcctg gccttcgtga acatggtggg cgggcaggac gagctggtgt tcgacggcgg
      421 gagcttcgtg gtgagcgagc gcggcgaggt cgtcgtgcgg gggcgcgcgt tcgaggagga
      481 cctggtcgtc gccgacctcg acctcgacgc cgtcttccac gcccgcctgc acgacccgcg
      541 tcgccggcag gagaagctcc gcgcgggcga cggggtcacc cgcgtcgcgc tcccggcgct
      601 gccggccccg gcggtgctgc ccgcgctggc gccgcgcgag gtgccggcgc tctccccggt
      661 cgccgaggtg ttccaggcgc tggtgctcgg cacgcgggac tacgtccgca agagcggatt
      721 caagcacgtc gtgatcgggc tttccggcgg catcgactcc tcgctggtgg cggcggtcgc
      781 cgtggaggcg ctcggtcgcg agaacgtcac cggcgtgacg atgccctcgc cgtactcgtc
      841 gggcggcacg aagagcgacg cgcgccggct ggcgagaaac ctcggcatcg agctcctccg
      901 actgcccatc acgcccgtct tcgcggcctt caagcgggcg ctggcccggc cgttcgaggg
      961 gctgaaggag gacgtcgcgg aggagaacat ccaggcgcgc gtgcgcggta cgctcctgat
     1021 ggcgctctcg aacaagttcg gctggctcgt gctcaccacc ggcaacaaga gcgagatcgg
     1081 cgtcggctac tccacgctct acggcgacat ggccggcggc ttcgccgtca tcaaggacgt
     1141 ccccaagacg ctggtgtacg agctgtcccg ccacctcaac gcgcgcgccg gccgcgagct
     1201 gatcccggag agcgtgttca ctcgcgcgcc gtccgcggag ctgcgtcccg accagaccga
     1261 ccaggacacg ctgccgccgt accccgtgct cgacgccatc ctcgaggcct acgtcgagga
     1321 cgaccgcagc gtggccgatc tggccgcgct cggcttcgac cccgagatcg cgcgcaaggt
     1381 ggtgcgcatg gtagacgcca acgagtacaa gcgccgccag gggccgatcg gtgtgaagat
     1441 cacgccgcgg gcgttcggcc gggactggcg cctgcccatc gtcaaccgct tccgccagcc
     1501 ctgaggccgc cggcggcgcc ccgcgtcttt ggcgcgtccc ggtgagccac gggctcggcg
     1561 gcgccctcga gcccgtggag gcccctccga ctatcgggtg ccggccggag tcatctgcag
     1621 cgcgtggagc cggcgataga ggccgtcgcg cgccagcagc tcgccgtggc ggccgacctc
     1681 ggcgatccgg ccttcgtgca cgaccacgat gcggtcggcg ttgcgcaccg tcgccagtcg
     1741 gtgcgcgatg accaggacgg tgcgcccctt catgaggtcg gcgagcgcct gctggatcat
     1801 gaactcgctc tcggcgtcga ggtcggacgt ggcctcgtcg aggatcagga tgggcgggtt
     1861 cttgaggaac gcgcgcgcga tggcgatgcg ctggcgctgg ccgccgctca ggcgcacgcc
     1921 gcgctcgccg accagcgtct ggtagccctc gggacaggcg acgacgaagt cgtgagcctg
     1981 ggcctggcgc gcggcgcgct cgatcgcctc gtccggcgcg tcgggctgcc cgtaggcgat
     2041 gttgtagcgg atggtgtcgc tgaagaggaa ggtctcctgc gtgacgatgc ccatcagcgc
     2101 ccggagcgag gcctgggtga tgtcgcgcac gtcgtggccg tccacggtga tgcgcccctc
     2161 ggtgacgtcg tggaaccggg gcagcaggtc catcagggtc gacttgccag ccccgctcat
     2221 gccgacgaag gccaccacct cgcccttccg gatcgtgagc gagatgtcgc ggagcgccgg
     2281 ctcggtgccc cgagcgtagc ggaacgccac ggcctcgaag cggatggcgt cgcggaactc
     2341 ctcgagggcg acggcccccg cgcggtcggc gacggccggc ggctggtcga cgatctcgaa
     2401 gacgcgctcc accgaggccg tcgtctgctg caccgtgttc agcgaccgcg agagccgccg
     2461 cgcggggccg tagagcatga tgacggcggc ggtgaacgag aagaacgtcc ccggcgtcat
     2521 gtcgccggcg atcacccggt agccgccgta caggatggcg gccacgatgc ccagcgcgcc
     2581 ggccacctcc atgagcggct cggtgatctc gtcggcccgg acgttccgga gcgagaggtc
     2641 cagcagccgg gcgttgatcg cgtcgaagcg ctgctgctcg tgggcttcgc ggccgaaggc
     2701 cttgacgatc ttcgtccccg agaacgcctc gtgcaggagc acggcgagct gcgccacgcg
     2761 ctcctggaat cgccggttga tcgcgtagag cttgcggccg atcacgcgca cgatgagcgc
     2821 gatgaacggg aaggccacga gcgcggccag tgtgaggagc cactcgcgag ccagcatcac
     2881 ggccaggagg gcgacgatgg tgccgacctg acgcaccgcc atgacgagga cggtcgaggc
     2941 caggcgcgcc aggcggttga cgtcgttgag gatccgcgac atcaggtccg ccgagtgcat
     3001 cccggcgaag aaggccagcg gcatcgactg gatgtgggtg tagaggtcgc ggcggagccg
     3061 ggcgaccacc ctctcgccca ccgcggccat gaggtaggcc tggaagtacc gggcggcgcc
     3121 cttgacgagg taggcgccga ggagcgccgg cggcaccagc ttcagcatcg ccgcgtcgcg
     3181 gcggatgaag atgtcgtcca tcacgggctt gacgagccag gcgatggcgc cgtccatcgc
     3241 ggccacgacg agggccaggg ccccgcccag gaccagcgtc ggcgtgtggg gccggatgta
     3301 cggcagcagg cggcggtagg gggcgagcag cctcacgcgc gctcccgctc ggcgagcagc
     3361 cggcggtaga gggtctcggt ggcgtccagc ttcgcccgcg tcgagaaccg cgcccgcacg
     3421 agctcccgcc cggcgcgcgc ggtggcccgg cggagggcgg ggtcctcgat cagacgcagg
     3481 atcgccacgg cgagggcggc ggggtcgcgc ggcggcacca gcaggccggt ctgcccgtcg
     3541 atcaccagct cggggttgcc ctcgatcgcg gtggcgacca cggccgtctc cacggcaagc
     3601 gcctcgcgca ccgtgccggt gaggccgagc ccggtgtacg aggcatccac gcagcagtcg
     3661 ctggccgcca ggatctcggg cacgtcctcg cggtagcccc acacgtgcgc gcggccctcg
     3721 agaccgagcg cgcgggcacg ctcgaggagc acctccgggc gccgcgcgcc gacgatcagc
     3781 aggtgcgcct gcggcgcgcg cgccgcgacc gcgcccatgg cgtcgacgac gtcgtcgttg
     3841 cccttccacg agcgcacgcc gatctgcgtc accaggaagg ccgccgggtc gagccccagc
     3901 ccgcgccgga tccccgagcc gtccacgccg ggatggaacc ggtccgtgtc cgtcccggag
     3961 tagatgacct ggatcttgtc ggcgcgcacg ccggaggcga tcagaccgcg ctggatggac
     4021 tcgcacacgg cgacgaccgc ggtgacccgg cgcgcgttgt agccgaggcg ggcgaagggg
     4081 tcgaggggaa aggagacccc gcggttgaga accagcaccg ggatgcgggc gaagagcccg
     4141 gccatgagcg agagcgtccg cgccttgccc ttctgggcgt gcacgacctc gatgcggtgc
     4201 ttgcggatga tccgcacgag ccgacgcacc gactgcaggt ccacctcgga agccatcggc
     4261 agcgcgtagt gggggagccc ggccgcgcgc gtcttctccg cccacgtctc gctcggccgc
     4321 gtggccagca ccacctcgtg accgcgctcg gcgagcccga ggcagagctc gcggagctgg
     4381 atggcggcgc cggtgaagta gtccgacttc gggtagagct ggaggatcct cacccgtgct
     4441 accgcccgaa gaaggccctc accgtggcga tgaccgtgtc gatctcggcg tcggtgtgat
     4501 tcggcgacat gggcagcgtg aggatctccc gcacgagctg ctccgtccgc ggcagcgccg
     4561 gcggagtcag cgtcgccatc gcgggctgcc ggtgcgtggg cacgggatag tggacgccgg
     4621 tctggatgcc gcgggccttg aggaaggcgg cgagcccgtc gcgctccggg gcgcggacga
     4681 cgtagagatg atagacgtgg cgcgcgtgct cgcgctcgtc gggcaggccg aggggcaatc
     4741 cggcgagccc cgccgcgtag cgcgcggcca gcgcccggcg gcgttcgttc atcgcgggaa
     4801 gccgccggag cagcacgcgc agcgccgccg cctgcagctc gctgaagcgc aggttgaagc
     4861 ccagctccgc gtgaacgtcc ttgctgagcc ggccatggtc gcgcaggcgg cggcagcgcg
     4921 cggcgatctc gtcgtcgctc gtgagcacgg cgccgccgtc gcccatggcg ggcaggttct
     4981 tggacgggta gaacgagagc acggcggcgc ggccgaagcc gcccaccgcg cgggcgtccc
     5041 aggccgcgcc ctgcgcctgc gcgcagtcct cgagcaccca gagaccgtgg cgcgtcgcca
     5101 cgtcctggat cgcgggcagg tccgcgggct ggccgtagag atgcacgggc aggatgccca
     5161 ccgcgcgcgg cgtgaccttc gcctgggcgt ccttgggatc catcgtgtgc cagtcgtcca
     5221 cgtcgacgaa caccggggtc ccgccggcgc agcagatggc ctcgatcgtc gggaacgccg
     5281 tgtgggcggg ggtcagcacc tcgtcgccgg gcttcacgcc gagggccttc atcgtcatcc
     5341 agagcgcggc cgtggccgag gtcgtgagca cggcgtggcg gagccccgcg tggcgggcca
     5401 gctcggcctc gagctcgcgg cactgcggcc cgaggatgta ctgccgcgag tccatcgcgg
     5461 ccagcacggc ctgcttgatc tcgtcgtcca cggggggacg ggacaggggg atcgtcacga
     5521 aggtcctccc tggagccggc cgtacttgcc gcggtagtag aggagcggct cgccctcacc
     5581 cccgcccgcc tcctccacgc ggccgacgaa gaccgtgtgg tcgccctcga cgtgcgcggc
     5641 cacggtgacg cactcgaggt gcaccagcgc gccctcgatg agcggcgtgc cgagcgtccc
     5701 gagccggtgg ggcaagccgt cgaacttgtc catacgggtg gtcgcgaagc ggcgcgacac
     5761 cgcctcctgg tcggtcgcca gcacgttcac ggcgaagcgc ccgctgtcgc gcagggccgg
     5821 ataggactgg gacttgtggt ccacgcagac gagcacgagc gggggatcga gcgagagcga
     5881 cgagaaggcg ctgaccgtga gtccggtggg tcgctggtcg gcgtcgtaag tggtgacgac
     5941 ggtcacgccg gtcgcgaaat ggccgaggac acggcgaaaa tcgtcggggc cgatcaccag
     6001 ttcgttctag caggtcgccc ggggcaatgc aacctcggac tccggtcccg gcccgtgcga
     6061 cgcccgggac cgtgcggtat agtgggcggc atatgatcac ggaacgggcg gttctcgacg
     6121 tcctgcgcac gatcaaggac cccgacggtc agcaagacat cgtcgcgctg gggctcgtgc
     6181 gcgacctcgc catccaggac acggaggtct ccttcacgct ggccttcacc acgcaagcgc
     6241 cagcgacgaa ggccgcgctg cacgccgccg cctcgcgcgc ggtggcacag gtgcccggcg
     6301 tggcgaaggt gaccgcgcgc atcggcgcgg cctcccgccc ggcgcccggc gggcacgccg
     6361 gccacgccga gcaggccggc catgcccacc atgccggaca ggcgccgggc gctgccggaa
     6421 gcgcgcaggc cgcggacctg atccccgagg tcaagcacac ggtcgccgtc tcctcgggca
     6481 agggcggagt gggcaagtcc acggtggccg tcaacctcgc catcgcgctg cggatggccg
     6541 gcgcgcaggt cggtatggtc gactccgacg tgtacggccc cgacgtgccg ctgatgctgg
     6601 gctcgcgcgg gaagccgggc atgttcgaga acaagatcat cccggtggag gcccacggca
     6661 tcaagatgat gtcgatcggg ctgctcgtcg gcgaccgcga gtcgctcgtg tggcgcggtc
     6721 cgatgatcca ctcgttcatc cagcagatgc tgcgcgacgt catgtggggg gcgctcgact
     6781 acctcgtctt cgacatgccg cccggcaccg gtgatgcgca gctctcgctc tctcaagtca
     6841 tcccgctgtc gggcgtggtg atggtgacga cgccgcagga cgtcgcgctg ctcgacgtgc
     6901 gcaaggccgt cggcatgttc cagcgcctca acgtacccat cctcggcatc gtcgagaaca
     6961 tgagctggtt cgcctgcccg cactgtggcg agcgcaccga gatcttcggg gacaaaggcg
     7021 gccggcgcct cgccgaggag tacggcgttc cgctcctcgc ccagctgccg ctcgatccgg
     7081 agacccgcaa gggcggcgac gagggagttc cgatcaccct gcggcggccc gacgcgccgc
     7141 aggcgcgcgc cttccgcgac ctgggcgcgg ccgtgcggcg gcgcctcgac gagctggccc
     7201 cgctgcggtc gctgcccaag atcggctgac ggcgggcgct cggccgatgt cgtctcccga
     7261 tccgacgccg ccggccgccg cgctcgtgct gccggttttt ccgctccccg acgttaccct
     7321 cttcccgcac gtgcccctgc cgctgcacat cttcgaggcg cgctatcgcg cgatggtgag
     7381 cgacgcgctg gcccgtgacc gcaggctctg cgtggcgcgg ctcgagccgg gctgggaggc
     7441 cggatacaac ggcaagcctc cggtggcccc gatcgcgggc gccggggaga tcgtttcgtg
     7501 ggagcggctg gcgaccggac gctacaacat cgtcgtcagg ggccagtggc gcgtgcggat
     7561 cgagcgggag ctgccgacgg acaccctcta ccgggtgatg cgaatgcggc ggctcgccga
     7621 cgaaccggcc gccgccgatc cggcgccccg cctcggccgc atccgtgccg cgtgcctgcg
     7681 gctgctcgac gcggtggggc ggccctcggg tctgctcgac cccttgctcg caccggagca
     7741 gccgcccggc gtcatcgccg accgggtggc cgccgcggtg ctgcccgacg cccgccagcg
     7801 tcaggagctg ctcgagacgc tcgacgtcgg cgtccggctg gagcggctgg cagcagcgct
     7861 ggacgcgctg gtcgaggagc tccggaaggg cgggaagcca tgacgccggc cgtccggctc
     7921 ctcgccctcg tcgccctgct ggtggcggcc tcggcggccg atgcacgctc gtgggcgtgg
     7981 ctcggcgtgc gcatccgcga catgactgag caggagatgg aggagatctc caaacgccac
     8041 ggcatccgcg agggcttcgg cgtcgtgatc gtcgaggtgc tcgagggcac gcccgccgcg
     8101 cgcgcggggc tgaagaacgg cgacatcgtg gtggcgttcg gcgagcgtcc agtgacggag
     8161 acc
//
LOCUS       DATIAZ010000041         4069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_141223, whole genome shotgun sequence.
ACCESSION   DATIAZ010000041 DATIAZ010000000
VERSION     DATIAZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141223"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VLF19_01395"
     CDS             complement(<1..850)
                     /locus_tag="VLF19_01395"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751870.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HSE91931.1"
                     /translation="MAISPLAGMPVPKAMLVDPAQLEREYHGRQPDLGDPAQLVGFGT
                     SGHRGSPLTGSFTEAHIAAITQAICDYRREHGTSGPLYMGKDTHALSEPAQRTALEVL
                     AANGVDTIVQRDHGVTPTPVISRAILAHNRHRTQHLADGIVVTPSHNPPEEGGFKYNP
                     PHGGPADTDATRWIEERANRLLRDGNQGVKRMPFAQAMTSPSTHEDDFILPYVRDLEN
                     VVDMPAIRGAGLALGVDPLGGAAGPVWEAINAVYKLDIAVVNPVIDPTFSFMTADHDG
                     KIRMDPS"
     gene            complement(862..3369)
                     /locus_tag="VLF19_01400"
     CDS             complement(862..3369)
                     /locus_tag="VLF19_01400"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HSE91932.1"
                     /translation="MRNRRTARARTPSAPGMQKLLEQYGCGPIEFTGSGDALYERHLM
                     FDNVVDPAAVGPRERYEAAARAVRDLLSQRWLRTENTYERENPKRVYYLSMEFLIGRS
                     LVNNITNMLIQPLARRVATDQQLDWLGVLEEEPDAGLGNGGLGRLAACFIDSMATMQL
                     PAMGYGLRYEYGIFKQAIQDGWQHEQPDNWLRRPDPWEVARPQERVEIRLNCSFEVRE
                     GTLRAVLGRPSRVFGLPFDRPVVGYGGKTINTLRLWAAAAPDAFDFQVFSGGEFVSAL
                     AEQLAAESVTRVLYPDDSTSMGQGLRFVQEHFLVACSLADLVRRFRRGNASWDALPAK
                     VAIQLNDTHPAMAVPELMRILLDEAHLGWDQAWDLTRRTLAYTNHTLLPEALEKWPLA
                     WFEMMLPRQLEIIFEINRRLLDDVRTRFPGDEGRVGRVSLVEEGRERKIRMANLAIVG
                     SHSTNGVAAIHSGLLRTVTVKDLAEMFPERFGNKTNGVTPRRWLLLANPALAGTITAA
                     IGDGWITDLGQLRRLTPLAGDAGFLDAFRKAKREAKSQFADWLKSTAGLTVDPESIFD
                     SQVKRIHEYKRQLLNALRIVVLYSRLRENPALPMTPRTFFFAGKAAPAYQLAKLVIKL
                     LNNLAGTIDGDPAVRGRLKVLFLPEYCVSLAERLIPATDVSNQISTAGYEASGTSNMK
                     FMMNGALTIGTRDGATIEMAQEAGEENFFLFGLTADQVAGSRGWYSPHWHYDNEPETR
                     AALDLIFSDHFSRNEPGVFAPLRGTLLTGGDHYMHLADLTSYLEADRRLVQLYTDPKG
                     WAHKAVLNVAASGKFSSDRTIAEYAAEIWDAKPCPVP"
     gene            complement(3412..4038)
                     /locus_tag="VLF19_01405"
     CDS             complement(3412..4038)
                     /locus_tag="VLF19_01405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSE91933.1"
                     /translation="MSVFAIRHGETEWSLSGQHTGTTDIPLTDNGRRLAELLRPALTR
                     QAFALVLVSPMQRARETCELSGLAGAAVVDPDLVEWNYGTYEGLTSGQIHEAAPGWLI
                     FRDGCPGGEAPGHVGARVDRVIARARAVEGDVALFAHGHVLRVLVARWLGLPAGAGQH
                     FLLDTGTLSVLGYYRGIPAVKTWNAPLAGNGVPASARREPEAMARSRG"
BASE COUNT          567 a         1395 c         1445 g          662 t
ORIGIN      
        1 tcgacgggtc catgcggatc ttcccgtcgt gatcggcggt catgaacgag aaggtcggat
       61 cgatcacggg gttcacgacc gcgatgtcga gcttgtagac cgcgttgatc gcctcccaga
      121 ccggcccggc ggcgccgccc agcggatcca cgccgagcgc gaggccggcg ccccggatgg
      181 ccggcatgtc gacgacgttc tccaggtccc ggacgtaggg caggatgaag tcgtcctcgt
      241 gcgtgctcgg cgacgtcatc gcctgcgcga acggcatccg cttcaccccc tggttgccgt
      301 cacgcaggag ccggttggct cgctcctcga tccaccgcgt cgcgtcggtg tcggccgggc
      361 cgccgtgggg cgggttgtac ttgaacccgc cctcctccgg ggggttgtgc gacggggtga
      421 ccacgatgcc gtccgcgagg tgctgcgtgc ggtggcggtt gtgagcgagg atggcgcgtg
      481 agatgaccgg cgtcggcgtc acgccgtgat cgcgctgcac gatggtgtcc accccgttgg
      541 cggcgaggac ctcgagcgcc gtgcgctggg ccggctccga cagcgcgtgg gtgtccttgc
      601 ccatgtagag ggggccgctg gtcccgtgct cgcgccggta gtcgcagatg gcctgggtga
      661 tggccgcgat gtgggcctcg gtgaacgagc ccgtgagcgg ggacccccgg tggccgctgg
      721 tgccgaagcc gacgagctgt gccgggtcac cgaggtcggg ctgccgcccg tggtactcgc
      781 gctcgagctg ggcagggtcc accagcatcg ccttcggtac cggcattcct gccaggggag
      841 agatcgccat cgtcgccctt cctagggcac cgggcagggc ttcgcatccc agatctcggc
      901 cgcgtactcg gcgatggtgc ggtcgctcga gaacttcccg gaggcggcca cgttgaggac
      961 ggccttgtgc gcccatccct tcgggtccgt gtagagctgc accagtcgcc ggtcagcctc
     1021 gagataggac gtgaggtccg ccaggtgcat gtaatggtcg ccgcccgtca gcagcgtgcc
     1081 gcgcagcggg gcgaagacac ccggctcgtt gcggctgaag tggtcggaga agatcaggtc
     1141 gagcgccgcg cgcgtctccg gctcgttgtc gtagtgccag tgcgggctgt accagccgcg
     1201 gctgccggcc acctggtctg cggtgaggcc gaacaggaag aagttctcct cgcccgcctc
     1261 ctgggccatc tcgatggtgg cgccatcgcg cgtgccgatc gtcagcgcgc cgttcatcat
     1321 gaacttcatg ttgctggtgc cgctggcctc gtagccggcg gtggagatct ggttggagac
     1381 gtcggtggcc gggatcaacc gctcggccag cgacacgcag tactcgggca ggaacagcac
     1441 cttgaggcgg ccgcgcaccg cggggtcgcc gtcgatggtg ccggccaggt tgttgaggag
     1501 cttgatgacg agcttggcca gctggtaggc cggcgccgcc tttccggcga agaagaacgt
     1561 gcgcggcgtc atcggcaggg ccgggttctc ccgcagccgg ctgtagagca ccacgatccg
     1621 gagcgcgttg agcaactgcc gcttgtactc gtggatgcgc ttgacctggc tgtcgaagat
     1681 ggactccggg tccaccgtca gcccggccgt cgacttgagc cagtcggcga actgcgactt
     1741 cgcctcgcgc ttggccttcc ggaaggcgtc gaggaaaccc gcgtcgccgg cgagcggcgt
     1801 gaggcgccgg agctgaccca gatccgtgat ccacccgtcg ccgatggccg cggtgatcgt
     1861 gccggcgagc gccgggttgg ccaggagcag ccagcgccga ggcgtgacgc cgttggtctt
     1921 gttgccgaag cgctcgggaa acatctcggc caggtccttg accgtcaccg tgcggaggag
     1981 accggagtgg atcgcggcca cgccgttggt gctgtgcgag ccgacgatgg cgaggttcgc
     2041 catccggatc ttgcgctcgc ggccctcctc cacgaggctc acgcgcccga cgcgaccctc
     2101 gtcgccgggg aaccgggtcc ggacgtcgtc gaggaggcgc cggttgatct cgaaaatgat
     2161 ttccagctgg cgcggcagca tcatctcgaa ccacgcgagc ggccacttct ccagcgcctc
     2221 gggcagcagc gtgtggttcg tgtacgcgag cgtccggcgg gtgaggtccc acgcctggtc
     2281 ccatccgagg tgcgcctcgt cgagcaggat ccgcatcagc tcggggacgg ccatggcggg
     2341 gtgcgtgtcg ttcagctgga tggcgacctt cgcggggagc gcgtcccagc tggcattgcc
     2401 gcggcggaag cgccgcacga gatccgccag ggagcaggcc acgaggaagt gctcctgcac
     2461 gaagcggagc ccctgcccca tgctcgtgga gtcgtcggga tagagcactc gcgtcaccga
     2521 ttcggcggcc agctgctcgg ccagcgcgct gacgaactcg ccgccgctga acacctggaa
     2581 gtcgaaagca tcgggcgccg ccgcggccca gagccgcagc gtgttgatgg tcttgccgcc
     2641 gtagcccacc acgggccgat cgaagggcag cccgaacacc ctcgagggcc ggccgaggac
     2701 ggcgcgcagc gtcccctcgc gcacctcgaa cgagcagttc agcctgatct cgacccgctc
     2761 ctgcgggcgg gcgacctccc acggatccgg gcggcgcagc cagttgtccg gctgctcgtg
     2821 ttgccagccg tcctggatgg cctgcttgaa gatcccgtat tcgtaccgca ggccatagcc
     2881 catcgccggg agctgcatcg tggccatgga gtcgatgaag cacgccgcca gacgcccgag
     2941 cccaccgttg ccgaggccag cgtcgggctc ctcctcgagc accccgagcc agtcgagctg
     3001 ctggtcggtg gcgacgcgcc tggccagcgg ctggatgagc atgttcgtga tgttgttgac
     3061 cagggagcgc ccgatgagga actccatcga gaggtagtag acccgcttgg ggttctcccg
     3121 ctcgtaggtg ttctccgtgc gcaaccaccg ctgcgagagg aggtctcgca cggcgcgggc
     3181 ggcggcctcg tagcgctccc gcgggcccac cgcggcggga tcgacgacgt tgtcgaacat
     3241 gaggtggcgc tcgtagagcg catcgcccga ccccgtgaac tcgatggggc cgcatccgta
     3301 ctgctcgagg agcttctgca tccccggcgc gctcggcgtt ctcgcccggg cggtcctgcg
     3361 gtttctcatg acgtcatcct ccccgcgtga ctgggggtcc ttctgtccag ttcacccgcg
     3421 cgatcgggcc atcgcctccg gttctcgtcg agcgcttgcc ggcactccgt tgccggccag
     3481 gggcgcattc caggtcttca cggcgggaat cccgcggtag tagccgagca cgctcaacgt
     3541 gcccgtatcc agcaggaagt gctggccggc gcctgcgggt agcccgagcc agcgcgcgac
     3601 gagcacgcgc agcacgtgcc cgtgcgcgaa cagcgcgacg tcgccctcga ccgcgcgcgc
     3661 ccgggcgatc accctgtcca ctcgcgcgcc gacgtgcccc ggcgcttcgc cacccgggca
     3721 cccgtcccgg aagatcagcc agcccggcgc cgcctcgtgg atctgccccg aagtcagccc
     3781 ctcgtacgtc ccgtagttcc actcgacgag gtcgggatcg acgacggcgg cgccggcgag
     3841 gcccgatagc tcgcaggtct cccgggcccg ctgcatcggg ctgacgagca cgagcgcgaa
     3901 tgcttgcctg gtgagcgccg gccgcaggag ctccgcgagc cggcgcccgt tgtcggtcaa
     3961 ggggatgtcc gtggtgccgg tgtgctgccc gctcaggctc cactcggtct ccccgtgcct
     4021 gatcgcgaac acgctcacga cgccgaccga acgctggtca tcaccgctg
//
LOCUS       DATIAZ010000042         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1325982, whole genome shotgun sequence.
ACCESSION   DATIAZ010000042 DATIAZ010000000
VERSION     DATIAZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325982"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..732
                     /gene="nthA"
                     /locus_tag="VLF19_01410"
     CDS             70..732
                     /gene="nthA"
                     /locus_tag="VLF19_01410"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008804890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HSE91934.1"
                     /translation="MSHDHHQDHDHVDAGPPLSDVQLRVRALESLLTDKGLVDPAALD
                     RLIDTYETKVGPRNGARVVAKAWVDPAYRQRLLADATAAIAELGYIGRQGEDMVVLEN
                     TPKVHNLVVCTLCSCYPWPVLGLPPVWYKAAPYRSRAVIDPRGVLREFGVEVGDDVEV
                     RVWDSTAELRYLVLPERPAGTEGMSEEELAELVTRDAMIGVARVAPPARDPAATGGGS
                     RR"
     gene            752..1426
                     /gene="nthB"
                     /locus_tag="VLF19_01415"
     CDS             752..1426
                     /gene="nthB"
                     /locus_tag="VLF19_01415"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008804891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HSE91935.1"
                     /translation="MNGVHDMGGMHGMGPIQAERDEPVFHSEWERRAFAINVASGFLG
                     KWNIDMSRYAREQMPPAEYLGTSYYEHWLWGLEKLLVDKGILTREEVAARLTGRPAPL
                     APPPSGLRVLKALDVAKALQNRRAARMDDPVPPRFKPGDHVVTKNMNPVGHTRLPRYA
                     RGRRGLVDRDHGVFVFPDAAAAERGRQPQHVYSVRFAGRELWGPEAPERDAIYIDLFE
                     PYLEPA"
     gene            1423..1779
                     /locus_tag="VLF19_01420"
     CDS             1423..1779
                     /locus_tag="VLF19_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HSE91936.1"
                     /translation="MRSPDLDQLPSIPRDAEGPVFREPWQAQAFGMTVVLHERGHFTW
                     REWTDRLTAEIAAAAARGDVDDGTRYYLYWLAALEKLVADKRLVLADELAVRKQEWDE
                     AARSTPHGQPIELRPR"
     gene            1853..2698
                     /locus_tag="VLF19_01425"
     CDS             1853..2698
                     /locus_tag="VLF19_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE91937.1"
                     /translation="MTIRLGEVTIDRVVEIDRSFYPTRDMLPESSPEVLARHLSWLKP
                     DFFDETVGDLGSRIQSYVVRTPQHTVLLDTGVGNDKERPESPLWHRRNGTYLDDLEAL
                     GIAPEQVDFVVCTHMHVDHVGWNTRLVDGRWVPTFSDATYLFAGEEWEYWRWEKDVCI
                     ADSVVPIVETGRAKLVATDHVIDPWLRFEPTPGHTPGHACVRLTTSGGQAVFAGDLMH
                     RTVQVAEPQWSSRFCWDGKRAAATRREFVECHADTGVLVAPAHFPHPGYVVREDGGFR
                     FVKAD"
     gene            2779..>3227
                     /locus_tag="VLF19_01430"
     CDS             2779..>3227
                     /locus_tag="VLF19_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91938.1"
                     /translation="MKALLTIIPLLFTAIGIFLGVIQYGRTQQEEFRKRYWDVQFKLY
                     SEATESAAAIANARQLSDVEKERRRFWQLYWGVLSAIEHPEVETAMKRFGTVLAECEA
                     GRRDSCPSPLPGAARTPLRSASYNLAHCVRASLAKTWRPVDLGDANY"
BASE COUNT          484 a         1119 c         1177 g          447 t
ORIGIN      
        1 gggatcgacg ccgggatgcg agccggcgtc cggtatgcga taaatgaggg ccgtgaaggg
       61 agggcccgga tgagccacga ccaccaccag gatcacgacc acgtcgacgc ggggccgccg
      121 ctcagcgatg tgcagctccg cgtcagggcg ctggagtcgc tgctgaccga caaggggctc
      181 gtcgaccccg ccgcgctcga ccggctcatc gacacctacg agaccaaggt ggggccccgc
      241 aacggcgccc gcgtcgtcgc gaaggcgtgg gtcgatccgg cctaccggca gcggctgctc
      301 gccgacgcca cggcggccat cgcggagctc gggtacatcg gccgccaggg cgaggacatg
      361 gtcgtcctcg agaacactcc gaaggtccac aacctcgtcg tctgcacgct gtgctcgtgc
      421 tatccgtggc cggtgctcgg cctgccgccc gtctggtaca aggccgcgcc ctaccgctcg
      481 cgcgcggtga tcgacccgcg cggggtgctc cgcgagttcg gcgtcgaggt cggagatgac
      541 gtcgaggtgc gcgtgtggga ctcgacggcc gagctgcgct acctggtact gccggagcgg
      601 ccggcgggta cggagggcat gagcgaggag gagctggcag agctcgtgac ccgcgacgcc
      661 atgatcggcg tggccagggt cgcgccgccc gcgcgggacc cggccgcgac cggaggcggg
      721 tcgcggcgat gagcacggtg gggtcgcgga gatgaacggc gtgcacgaca tggggggcat
      781 gcacggcatg gggccgatcc aggccgagcg tgacgagccc gtcttccaca gcgagtggga
      841 gcggcgcgcg tttgcgatca acgtcgccag cggcttcctc gggaagtgga acatcgacat
      901 gtcgcgctac gcgcgcgagc agatgccgcc ggccgagtac ctcgggacgt cgtactacga
      961 gcactggctg tgggggctcg agaagctgct cgtggacaag ggcatcctca cgcgcgagga
     1021 ggtcgcggcg cgcctgacgg gccggccggc gccgctggcc ccgccgccgt cgggtctgcg
     1081 tgtcttgaag gcgctggacg tcgccaaggc gctccaaaac cgccgggccg cgcgcatgga
     1141 cgatccggtc ccgccgcggt tcaagccggg cgaccacgtg gtcacgaaga acatgaaccc
     1201 cgtcggtcac acgcggctgc cgcgctacgc gcgcggccgg cgcggcctgg tcgaccgcga
     1261 tcacggcgta ttcgtgttcc ccgacgcggc cgcggcggag cggggccggc agccccagca
     1321 cgtctacagc gtgcgtttcg ccgggcgcga gctgtggggg ccggaggccc ccgagcggga
     1381 cgcgatctac atcgacctgt tcgagccgta cctggagccc gcatgagatc gcccgacctc
     1441 gaccagctcc cctcgattcc gcgcgacgcc gagggcccgg tgttccgcga gccgtggcag
     1501 gcgcaggcct tcggcatgac cgtcgtgctc cacgagcggg gccacttcac gtggcgcgag
     1561 tggacggacc gcctcaccgc cgagatcgcc gctgccgccg cgcgaggcga cgtggacgac
     1621 ggcacccgct actacctgta ctggctcgcc gccctcgaga agctcgtcgc cgacaagcgg
     1681 ctcgtgctgg ccgacgagct ggccgtgcgc aagcaagagt gggacgaggc cgcgcgctcg
     1741 acgccccacg gccagcccat cgagctgcgc ccgcgctgac gctcctgtcg acggcctgcg
     1801 cctcggcggc acgtgccgcg agcacacggg gaaggtcagg gaggacgctt ccatgacgat
     1861 caggctgggc gaggtcacca tcgaccgtgt cgtcgagatc gaccgctcct tctaccccac
     1921 gagggacatg ctgccggagt cgtcgccgga ggtgctggcg cgccatctgt cctggctcaa
     1981 gccggacttc ttcgacgaga ccgtcggcga tctcggctcg cggatccagt cgtacgtcgt
     2041 ccgcacgccc cagcacaccg tgctgctgga tactggcgtc ggcaacgaca aggagcgccc
     2101 cgaatccccg ctctggcacc ggcgcaacgg cacgtacctg gacgacctcg aggcgctggg
     2161 gatcgcgccg gagcaggtgg atttcgtcgt ctgcacgcac atgcacgtgg atcacgtcgg
     2221 ctggaacacg cgtctcgtgg acggccgctg ggtgccgacg ttctccgacg ccacgtatct
     2281 cttcgccggc gaggagtggg agtactggcg ctgggagaag gacgtctgca tcgccgacag
     2341 cgtggtgccg atcgtcgaga ccggccgggc gaagctcgtg gccaccgacc acgtcatcga
     2401 cccgtggctc cgcttcgagc ccacgccggg gcacacgccg ggtcacgcct gcgtgcggct
     2461 gacgacgagc ggcggccagg ccgtgttcgc cggcgacctc atgcaccgca cggtgcaggt
     2521 ggccgagccg cagtggtcga gccggttctg ctgggacggc aagcgcgccg ccgccacccg
     2581 ccgcgagttc gtggagtgcc acgccgacac cggcgtgctc gtggcgccgg cgcacttccc
     2641 gcaccccggc tacgtggtgc gcgaggacgg cgggttcagg ttcgtcaagg cagactgagt
     2701 ctcgagcacc tcgggaagag ctggtcccac ggcgcggcct gaggtccgcg acctcgggcg
     2761 cccccggagg tgagcctgat gaaagcgctg ctgaccatca ttcccctgct gttcacggcg
     2821 atcggcatct tcctcggcgt catccagtac ggccggacgc aacaggagga gttccgcaag
     2881 cggtactggg acgtgcagtt caagctgtac agcgaggcga cggagtcggc ggcggccatc
     2941 gcgaatgccc ggcagctctc ggacgtcgag aaggagcgcc ggcgcttctg gcagctctac
     3001 tggggggtgc tctccgccat cgagcacccc gaggtcgaga ccgcgatgaa gcggttcggg
     3061 accgtgctgg cggagtgcga agcgggcagg agggactcgt gcccgtcgcc gctgcccggg
     3121 gcggcacgta cgccgctccg cagcgcgtcc tacaacctgg cgcactgcgt ccgcgcgtcg
     3181 ctggccaaga cctggcgccc cgtggatctc ggcgacgcga actactg
//
LOCUS       DATIAZ010000043         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1457279, whole genome shotgun sequence.
ACCESSION   DATIAZ010000043 DATIAZ010000000
VERSION     DATIAZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457279"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VLF19_01435"
     CDS             complement(<1..312)
                     /locus_tag="VLF19_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSE91939.1"
                     /translation="MIRSVPGRTPRIHPGAWVDPAAQVIGDVEVGDASSIWPGTIVRG
                     DQDNYVTIGRNSNVQDCSVLHVTPQNPCIVGDNVTIGHRCVVHACTVKDNVRIGIGAV
                     VL"
     gene            complement(315..1499)
                     /locus_tag="VLF19_01440"
     CDS             complement(315..1499)
                     /locus_tag="VLF19_01440"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSE91940.1"
                     /translation="MAEAVIVSTARTPIGRAYRGAFNNTHGATMGGHVIRHAVERARI
                     DPGEVEDVIMGCALPEGATGQNIARQCALRAGLPVSTGGVTVNRFCSSGMQAIAMAAH
                     RVVVDRVPIMVAGGLESISLVQNEHFNNHRIHEPWIDEHKPELYMPMIDTAEVVARRY
                     YIAREAQDEYALASQQRTAAGQKDGRFDIEIVPLATTKLVQDKNTGEIREEEVTLTKD
                     EGNRPDTTLEGLAKLAPVRGEKGFITAGNASQLSDGASACVVMDARLAERRGLQPLGV
                     FRGFTVAACEPDEMGIGPVFAVPRLLERFGLEMDQVDLWELNEAFAVQVVYCRDRLGI
                     PMERLNVDGGAISIGHPYGMSGARMVGHALIEGKRRGARHVVCTMCIGGGMGAAGLFE
                     VA"
     gene            1671..2399
                     /locus_tag="VLF19_01445"
     CDS             1671..2399
                     /locus_tag="VLF19_01445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE91941.1"
                     /translation="MSPAGEWSEADSRAFLDAGDVFVPDRHEQVRTLCALVPAARDET
                     FTAVELGAGGGVLARAVLEAFPRCRYLALDGSPAMREHLAAALARHGPRVEVRDFDLA
                     AQDWRDALSPVRCVLASLVVHHLDATGKRALFADLGQRLGPGGALLLADLVEPPTRAA
                     RTLFAQQWDDAARAQSLARTGGLEAYRRFERERRNFYRDPEPDPLDQPSRLLDQLDWL
                     RESGFTRVDCFWMRAGHAIYGGYR"
BASE COUNT          331 a          939 c          865 g          389 t
ORIGIN      
        1 gagcacgacg gcgccgatgc cgatgcggac gttgtccttg accgtgcatg cgtgcacgac
       61 gcaccggtgc ccgatggtga cgttgtcgcc gacgatgcag gggttctgcg gagtgacgtg
      121 caggaccgag cagtcctgca cgttcgagtt gcgcccgatc gtgacgtagt tgtcctggtc
      181 cccacgcacg atcgtgccgg gccagatgct ggaggcgtcg ccgacctcca cgtccccgat
      241 aacctgcgcg gccggatcca cccacgcccc gggatggatc cggggcgtgc ggccgggcac
      301 cgagcggatc acgctcaggc gacctcgaac agccccgccg cccccatgcc cccgccgatg
      361 cacatcgtgc agacgacgtg acgcgcgccg cggcgcttgc cctcgatgag ggcgtggccg
      421 accatgcgcg cgccgctcat cccgtacggg tggccgatgg agatcgcccc gccatccacg
      481 ttgaggcgct ccatgggaat gccgagccgg tcccggcagt acaccacctg caccgcgaac
      541 gcctcgttca gctcccagag gtcgacctgg tccatctcga ggccgaaccg ctcgagcagg
      601 cggggcaccg cgaacaccgg gccgatgccc atctcgtctg gctcgcaggc ggccaccgtg
      661 aagccccgga agacgccgag cggctggagc ccgcggcgct cggcgagcct ggcgtccatg
      721 acgacgcatg ccgaggcccc atcggagagc tgactggcgt tgcccgcggt gatgaagccc
      781 ttctcgccgc gcaccggcgc cagcttggcg agcccctcga gggtggtgtc gggacgattg
      841 ccttcgtcct tcgtcagcgt cacctcctcc tcgcggatct cgccggtgtt cttgtcctgc
      901 acgagcttcg tcgtcgccag cggcacgatc tcaatgtcga agcggccgtc cttctggccc
      961 gcggcggtgc gctgctggct ggccagcgcg tactcgtcct gcgcctcgcg cgcgatgtag
     1021 tagcgccggg ccacgacctc cgccgtgtcg atcatgggca tgtacagctc gggcttgtgc
     1081 tcgtcgatcc acggctcgtg gatgcggtga ttgttgaagt gctcgttctg cacgaggctg
     1141 atcgactcga ggccgccggc gaccatgatc ggcacccggt cgacgacgac ccggtgggcc
     1201 gccatggcga tggcctgcat cccggaagag cagaagcggt tgacggtgac cccgccggtg
     1261 ctcaccggca gccccgcccg gagcgcacac tggcgcgcga tgttctgccc cgtggcgccc
     1321 tcgggcagcg cgcagcccat gatgacgtcc tcgacctcgc cgggatcgat ccgcgcgcgc
     1381 tcgacggcat ggcggatgac gtggccgccc atggtcgcgc catgggtgtt gttgaaggcg
     1441 ccgcggtagg cccgcccgat cggggtccgg gcggtcgaga cgatcacggc ctctgccatg
     1501 gatgggcctc cggaaagagt ggcttgcgcc gatcatacac ccccggcccg tcgccggctc
     1561 ccctggcgcc gcgggtcggc tctcggctac cctgccctca tgcgcatcac gggaaaagtc
     1621 gcgcccgttt ccggcgtcgc cttgcccgtc gacgggggcc acgcggcggc gtgagccccg
     1681 ccggcgagtg gagcgaggcc gactcgcgag cgttcctcga cgcgggcgac gtgttcgtgc
     1741 cggatcgcca cgagcaggtc cgcacgctct gcgccctcgt cccggcagcg cgcgatgaga
     1801 ccttcacggc cgtcgagctg ggcgccggcg gcggagtgct ggcgcgcgcc gtgctcgagg
     1861 cgttcccccg gtgccgctac ctggcgctgg acggctcccc ggcgatgcgc gagcatctgg
     1921 cggcggcgct ggcgcgtcac ggcccacggg tcgaggttcg cgacttcgac ctcgcggcac
     1981 aggactggcg cgacgccttg tcgcccgtcc gctgcgtgct cgcctcgctc gtcgtccatc
     2041 acctggacgc gacgggcaag cgcgcactgt tcgccgatct cggccagcgg ctcggaccgg
     2101 gcggcgcgct gctgctcgcc gatctggtcg agcccccgac gagagcggcg cgcacgctct
     2161 tcgcccagca gtgggacgac gccgcccgcg cgcagagcct ggcgcgcacc ggcgggctcg
     2221 aggcctaccg ccggttcgag cgcgagcgcc ggaacttcta tcgcgacccc gagcccgacc
     2281 cgctcgacca gccctcgcgc ctgcttgacc agctcgactg gctccgcgag tctggattca
     2341 cccgcgtcga ctgcttctgg atgcgcgccg gccacgccat ctacggcggc taccgctgat
     2401 ttcccacgtc caaccatctc gcgacgtccg ggtggcggcc ggaaagccgc gatatcgccc
     2461 gatctcgagc ggctggccac gttacgcagc tgcccgaggc tcatcacaca gctcccgtcc
     2521 cggg
//
LOCUS       DATIAZ010000044         5942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_10550, whole genome shotgun sequence.
ACCESSION   DATIAZ010000044 DATIAZ010000000
VERSION     DATIAZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10550"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            608..1867
                     /locus_tag="VLF19_01450"
     CDS             608..1867
                     /locus_tag="VLF19_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE91942.1"
                     /translation="MSDTDVRPSAWRAIAAATALNAPLGSLYAFSVFLKPLETLLGLS
                     RADLALVFGLASAGFGAGMNLAPHVYGLASAPMLVLACAAASTLGIALAATAGGLAQL
                     AIGYGVLFGAGGGAAYILVQQAVNLAVTRRHGLVNGYIVALYPAGAMIAAPLFGWAVR
                     ELGVRITFGGLATILAVTGSISAWLIAHSGVTLTAAAASAAPGEAERRRLVFWRLWLV
                     FFLAASAGLMVLSQAAGIITAYGGATRLAVYGTTFIAGSIAAARLGGGWMVDWLTIPA
                     VAAGAHAVALAGSVALTLWPGPEVSVLALTLVGLGYGVISGVTAAAMAVYWRRALYGR
                     VASRIYLAWCAAAIVLPIAAGRLFDLTQSYGTAILIAAGGNALGVIVALGLPRQRTPR
                     AEARLRSGQAHVKAAGPTRPANSLSAG"
     gene            complement(2486..3112)
                     /locus_tag="VLF19_01455"
     CDS             complement(2486..3112)
                     /locus_tag="VLF19_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3068..3070),aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HSE91943.1"
                     /translation="MTRPVTMRIFSDYVUPWCYLSTVRIERLKAEHDVKVEWVHFPLH
                     PDTPPEGRSLAALFAGRNVDRKAMHAQMKARMDAEGLPYGERTMTYNSRLAQELGKWA
                     DTQPGGEAIHDALFRAYFVEARDISQPAVLLDIVQHVGLSVDDAREVLEKRTFKDAVD
                     QDWELSRRYGVTGVPTFVAGRHGVVGAQPYEVLEQLVRKAASQEDAEG"
     gene            complement(3127..4089)
                     /locus_tag="VLF19_01460"
     CDS             complement(3127..4089)
                     /locus_tag="VLF19_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HSE91944.1"
                     /translation="MSVILGTGSHRYEVVDRWAKLPPGREFNADVAAVGVDRQDRVYA
                     FNRGQHPMVVFDRDGNFLRSWGEGVFRRAHGVHMAPDDTLWLTDDGDHTVRHCTLDGK
                     VLLTIGIPGTPKPYMSGEPFHRCTHTTLSPQGDLYVSDGYGNSRVHKFAPNGTLLSSW
                     GEPGTDPGQFNIPHNICCDADGWVYVADRENHRVQVFDGNGRYETQWNNMHRPSGLFL
                     QSGPDPRFYVGEIGGGVAVNYDVPNIGPRVSIYSPQGEVLARLGRGPAGLEPGQFMSP
                     HGLAVDSRGDIYVGEVSFTNWRNRYKDQPHPPGLRSLQKLVRVG"
     gene            complement(4183..4416)
                     /locus_tag="VLF19_01465"
     CDS             complement(4183..4416)
                     /locus_tag="VLF19_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91945.1"
                     /translation="MHDAIAAESRGIPAVAVMTDRFVPSARAVAELNGLPDYPFVVIA
                     HPIANDGDEALRSKAEAAVTRIVALLTERAASP"
     gene            complement(4438..4719)
                     /locus_tag="VLF19_01470"
     CDS             complement(4438..4719)
                     /locus_tag="VLF19_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91946.1"
                     /translation="MTLQVLDPRLSAEGEAARLAAPLASLDGALVGLLDNAKIGTARF
                     YDHLEAILRARFGVREVIRRRKPDSSRPAPADVLGELSAADAIVSGIGD"
     gene            complement(4716..5750)
                     /locus_tag="VLF19_01475"
     CDS             complement(4716..5750)
                     /locus_tag="VLF19_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91947.1"
                     /translation="MATLQSPLHEVADAFEANELFQRNGWTDGLPIVPPTATGVRRFL
                     EAAGLSPDVVVGVEPVRRRRITAEKAAIAAVMAGCLPEYLPVVVAVVTAMCEPEFGLH
                     GVTASTGGSAPFVVVNGPVRLAIGMNATHNVLANGSRPNATIGRALRLLIINVIGGIP
                     GQLDRSTLGHPGKFTLCLAEDEEDSPWLPLATERGVPPGASAVTVLAVESPHQIMNEW
                     THDPRELAETYAAAIRANMLTYSIWAGNYAIVVPRQHRQIFAEAGWSKRDLRECVHER
                     ARVARREWRTVGKGAVAPRKDEDAVYTALRSPDDLLVVAAGGPAGGFGAILPPWYGKK
                     SLAVTKGLER"
BASE COUNT          818 a         2100 c         2081 g          943 t
ORIGIN      
        1 tccgatcggg tccgcgcggc accctgcagg tggcctcggt caccgacgtg cgaccggtgc
       61 gccccgcgaa caccggcgcg tcgaggtgct tctggtacca gagctgcgcg cagaacggat
      121 cgtcctggaa catggctgca cgcccttggc cctggcctcg gcgatctgcg ccttcgtcag
      181 gccgggcgtg ctgcccgtgc tcggccaggt gtcgctgctg acgaggaccg agccgccttc
      241 atccgtggtg tagaggggca agagcctgac gtccggcggc gccgcgaggg ccggcggacc
      301 gccccagctc gccttcgccg aggtcgggaa ctgccggatg tagaaatcgg ccgccgcatc
      361 gggatcgacg gagttcaggt gcagatgatg gaagcccgga gccgcacgca tgtcagcctc
      421 cctggccgct tcctactcgg gaacgcggcg ctggtcaagc tccaggggtt catggaatat
      481 atccgagcga tcggctggac aaggccctcg cttccaatcg cccggccgtc gtggaggtca
      541 tcggtgacgt cgacgcgatg tccaaacggg cgtggagacc ttccgcaaca tagaggctcc
      601 gatcgtcgtg agcgacactg acgtccggcc gagtgcgtgg cgcgcgatcg cggcagccac
      661 cgcgctcaat gcaccgctcg gctcgctcta cgcgttcagc gtgttcttga agccgctgga
      721 gactctactc gggctgagcc gggccgatct cgcgctcgtc ttcggcctgg catcggccgg
      781 gttcggcgcc ggcatgaacc tggcgcccca cgtctacggc ctcgcgtcgg cgccaatgct
      841 cgtgctggcc tgcgccgccg cgagcacgct gggtatcgcg ctggccgcca ccgccggtgg
      901 cctggcccag ctggccatcg ggtacggggt gctcttcggc gccggtggcg gcgcggccta
      961 catcctcgtg cagcaggccg tgaacctggc ggtgaccaga cgccacgggc tggtgaacgg
     1021 ctacatcgtc gccctgtatc cggcaggcgc catgatcgcc gcgcccctct tcgggtgggc
     1081 cgtccgcgag ctcggcgtgc gcatcacttt cggagggctc gccacgatcc tggccgtcac
     1141 ggggtcgatc agcgcgtggc tgatcgcgca ttccggcgtc accctcacgg cggcggccgc
     1201 gagcgccgca ccgggcgagg cagagcgacg ccggctcgtg ttctggcggc tgtggctcgt
     1261 cttcttcctg gcggcatccg ccggcttgat ggtgctcagt caggccgccg gcatcatcac
     1321 ggcctatggc ggcgcgaccc ggctcgccgt ctacggcacc acgttcatcg cgggcagcat
     1381 cgccgccgca cgcctgggcg gcgggtggat ggtcgactgg ctcaccattc ccgcggtcgc
     1441 ggccggcgcc cacgccgtcg cgctcgccgg cagcgtggcg ctgacgctct ggcccggccc
     1501 cgaggtatcg gtcctcgcac tgacgctggt ggggctgggc tacggcgtga tctccggcgt
     1561 caccgcggcc gcgatggccg tctactggcg acgcgcactg tatggccggg tggccagccg
     1621 catctacctg gcgtggtgcg ccgcagccat cgtccttccg atcgccgccg gccgcctgtt
     1681 cgacctgacg cagagctatg ggacggccat cctgatcgct gccggcggca acgccctggg
     1741 cgtgatcgtg gcgcttggac tgccgcgtca gcgcacgcca cgggccgaag ctcggcttcg
     1801 atccggccag gcgcacgtga aggcggccgg cccgactcgg cccgcgaaca gcctctccgc
     1861 tggatgacct ggcgcgagcg gtgcttgggc catcgacccg cgagagccgg gcgagccggc
     1921 cggcggacat cacttgcatc cgatcttcga ggcggaggag actgcccgaa cgatcggtgg
     1981 tacgggctgg cgctggaagc tgcggcgcgc tcggagccgg ccgtctcgcg tcgtcggctc
     2041 gccgaggccc ggtggcggct ggccgccacc gggtctcggc cgggaggcga aggcctatcg
     2101 acccgtcgct ggcgacgccg cggtgggcgt tgactcggga gcgttggcga ctaccgggta
     2161 ctttgcgcac gccgaaagcc cggcgagcaa cgcgatcagt gcgagcacga acatcgtcga
     2221 tttcagtgtc gagtacatgc gttgttcccc ccttggatcg ccgccgatgg gcgcgtctcg
     2281 gttgaactca ctcatgccgg cgtcggggtg cacgcgcagt gccagtccca caaactgtcg
     2341 acgaatcaag gctgagggtg tgcttcatgg cctggcgcgt gacagccagg cgtggacagg
     2401 tgaccggagt tgtcagccgg cgagctaccc gccaggaggc ccgtctgccc gtgcgacgcc
     2461 ccgcggcgtc ctggcccgcg gcgtcctagc cctcggcatc ctcctgcgac gcggccttcc
     2521 gcacgagctg ctccagcacc tcgtatggct gcgcgccgac gacgccgtgg cgcccagcga
     2581 cgaacgtcgg cacacccgtc acgccgtacc ggcgcgagag ctcccagtcc tggtcgaccg
     2641 cgtccttgaa cgtccgcttc tccagcacct cgcgggcgtc gtcgaccgac aggcccacgt
     2701 gctgcacgat gtccaggagc accgccggct gggagatgtc gcgcgcctcc acgaagtagg
     2761 cgcggaacag cgcgtcgtgg atggcctccc cgcccggctg cgtgtccgcc cacttgccca
     2821 gctcctgggc cagccggctg ttgtacgtca tggtgcgctc gccgtagggg agaccttcgg
     2881 cgtccatgcg cgccttcatc tgcgcgtgca tggctttccg gtccacgttg cgccccgcga
     2941 acagagccgc cagcgagcgg ccttccggcg gggtgtccgg atgcagggga aagtgcaccc
     3001 actcgacctt cacgtcgtgc tctgccttga gccgctcaat acgcacggta ctgagatagc
     3061 accagggtca gacgtagtcg gagaagatcc tcatcgtcac cggccgggtc atgacgcctc
     3121 ctgtcttcag cccaccctga cgagcttctg caagctgcgc agcccgggcg ggtgaggctg
     3181 gtccttgtag cgattgcgcc agttggtgaa cgacacctcg cccacgtaga tgtcgccgcg
     3241 cgaatcgacg gcgagcccgt gcggcgacat gaactggccc ggctcgagcc cggccgggcc
     3301 acggcccagc cgcgccagca cctcgccctg ggggctgtag atgctgactc gcgggccgat
     3361 gttgggcacg tcgtagttga ccgccacgcc gccgccgatc tcgccgacgt agaagcgcgg
     3421 gtcggggccg gattgcagga acagccccga gggacggtgc atgttgttcc actgcgtctc
     3481 gtacctgccg ttgccgtcga agacctggac gcgatggttc tcgcggtcgg cgacgtacac
     3541 ccagccgtcg gcgtcgcagc agatgttgtg cgggatgttg aactggcccg gatcggtgcc
     3601 gggctcgccc cacgaggaga gcagcgtgcc gttcggcgcg aacttgtgga cgcgcgagtt
     3661 gccgtagccg tcggagacgt agagatcgcc ctgcggcgac agcgtcgtgt gcgtgcagcg
     3721 gtggaacggc tccccgctca tgtagggctt cggcgtcccg gggatgccga tcgtcaggag
     3781 caccttgccg tccagcgtgc agtgcctgac ggtgtggtcg ccgtcgtcgg tcagccagag
     3841 ggtgtcgtcg ggcgccatgt gcacgccgtg agcgcggcga aacacccctt cgccccagga
     3901 gcgcaggaaa ttgccgtcgc ggtcgaacac gaccatcggg tgctgacccc gattgaacgc
     3961 gtacacccga tcctgccggt cgactccgac cgcggcgacg tcggcgttga actcgcgtcc
     4021 cggcggcagc ttcgcccacc gatcgaccac ctcgtaacga tgcgaccccg tgcccaggat
     4081 gaccgacatc gcgcatcccc tctctccggc ggtgacttgc gccgattata ggccgaggcg
     4141 gcgcgactcg atgccgggag gatctggatg gctgcggagc tcctacggcg aggcggcgcg
     4201 ctcggtcagc agggcgacga tccgcgtcac cgcggcttcc gccttgctcc tgagcgcctc
     4261 gtcaccgtcg ttcgcgatcg ggtgggcgat cacgacgaac gggtagtcgg gcaggccgtt
     4321 cagctcggcc acggcgcggg cgctcggcac gaagcgatcc gtcatgacgg cgacggccgg
     4381 gatgccccgg ctctccgcgg cgatggcgtc gtgcatactg cacgacgagc agcttcctca
     4441 atcgccgatg cccgagacga tcgcgtccgc ggcgctcagc tcgcccagca cgtcggccgg
     4501 agctggccgg ctcgaatcgg gcttgcggcg gcgaatgacc tcacgcacgc cgaagcgggc
     4561 gcgcaggatc gcctccagat gatcgtagaa gcgggcggtg ccgatcttcg cgttgtcgag
     4621 caggcccacc agggcgccgt cgagcgacgc gagcggggcc gcgaggcgcg cggcctcgcc
     4681 ttcggcggac agccggggat cgaggacctg gagcgtcatc gttcgagtcc tttcgtcacg
     4741 gccagggact tcttgccgta ccacggcggc aggatcgcgc cgaatccgcc ggcggggcca
     4801 ccggcggcga ccaccaggag gtcgtcgggc gagcggagcg cggtgtagac ggcgtcctcg
     4861 tccttccggg gggcgacggc gcccttgccg accgtccgcc actcgcggcg cgccacgcgg
     4921 gcgcgctcgt ggacgcactc ccgcagatcc cgcttgctcc acccggcctc cgcgaagatc
     4981 tgccggtgct gcctgggcac cacgatggcg tagttccctg cccagatcga gtacgtgagc
     5041 atgttggcgc ggatcgccgc cgcgtacgtc tccgcgagct ccctcgggtc gtgcgtccac
     5101 tcgttcatga tctggtgcgg cgactcgacc gccaggaccg tgaccgcgga cgctcccggg
     5161 gggacacccc gctcggtggc gagcggcagc cacgggctgt cctcctcgtc ctcggccagg
     5221 cagagggtga acttccccgg gtgaccgagc gtcgagcggt cgagctggcc cggaatgccg
     5281 ccgataacgt tgatgatgag caagcgcagc gcacggccga tcgtcgcgtt gggccggctg
     5341 ccattggcga ggacgttgtg ggtcgcgttc atgccgatgg cgagccgcac cgggccgttc
     5401 accaccacga acggcgcgct ccctccggtg ctggcggtga cgccgtgaag cccgaactcc
     5461 ggctcgcaca tggccgtgac gaccgccacc acgaccggca ggtactccgg caggcagccg
     5521 gccatcaccg cggcgatcgc ggctttctcg gcggtgatcc ggcggcgccg gaccggctcg
     5581 acaccgacga ccacgtcggg ggaaaggccg gcggcctcga ggaagcgccg caccccggtc
     5641 gccgtgggcg gcacgatcgg cagcccgtcg gtccatccgt tccgctggaa cagctcgttc
     5701 gcctcgaacg cgtcggccac ctcatggagg gggctctgca gcgtcgccat catctcgcct
     5761 cgctcgcctc ggccatcgcg ccctgcctat cttcgctcac ccgagagcaa agtccaatcc
     5821 gcgccacggc ttgcgggacg ctccggtggg cggcggctcc tcccggcatc ggtcgctttc
     5881 acatccggcg cggaccggtg tctcataggg cgtgggagag agtgtcacat tgaccgccga
     5941 ga
//
LOCUS       DATIAZ010000045        12842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_405314, whole genome shotgun sequence.
ACCESSION   DATIAZ010000045 DATIAZ010000000
VERSION     DATIAZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12842
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405314"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VLF19_01480"
     CDS             <1..833
                     /locus_tag="VLF19_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE91948.1"
                     /translation="LVHAGHHLAYDRYSYLACLGWALLAGGGIAAAWEARARVRPAFA
                     RAAVAAAAVGALGLGVITVYQIRVWHNTETLWRHAVAVDPGCSICRDNLGVALYNANR
                     IDAARAEFETALALRPDRARARLNLGVALVALGEPERAEAEFARVVSAHPTSASALNA
                     LGVALIRQRRPEAALTYLERAVALAEGDPYARTHLAVALTAAGRPGDALGHYRRAIEL
                     LPDAGLPRLGLARAHLALGDLRGAREHYEALKTIDARLAGQLALEMHASIQGGAGERF
                     "
     gene            820..4011
                     /locus_tag="VLF19_01485"
     CDS             820..4011
                     /locus_tag="VLF19_01485"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSE91949.1"
                     /translation="MNASDRQRGRVLTGVLVCFFFSGFTGLVYEVLWIRMLGLVFGQT
                     VFAITTVLAAFMGGLGLGSFLFGRVVDRDQRPLRTYGLIEIGIGVSCLLVPVVLPWVS
                     TLYLGLHRALGLSYFGFSLTQFVLVLAVLLVPTTLMGGTLPVLSRFFARDAVTLSRRV
                     GLLYALNTFGAVAGTAAAGYGLLPLFGVRGTLLLAATLTIGVGALAIVFSAHMDRLAS
                     DAPARETEPAPDPQGQPAGVGRLAGVVTLGLGVSGASSMIYEIGWSRALALVLGSSTY
                     AFTAMLVAFLVGIAGGSALFSRLRASRAATAGTFALIQLGIATSALLILPAFEWMPRA
                     LLRALTLSLDPGFVMAAQIVMSIAAMIVPTLLIGASFPCAIDLVARRVERAGFDVGRL
                     YSVNTLGAIVGTVLAGFLLIPALGAQTTVKVAVALNALTGLAIAVTGGQAVRRPQWAG
                     IGALAAVTAAAIGWIPAWNAAAMTTGVAVYGHVYRSAPSLARGREVLLYEDGLTATVS
                     VHRAGGNISLRVNGKTDASNGADMHTQTMSAHLPLLAHPDPKSVLVIGLASGVTVGAA
                     AQHPVERIDVVEIEPAMRRAAQFFADENRRVLDDPRVRVIFADGRNFLLAATQRYDVI
                     ISEPSNPWIRGVATLFTREFYTLARSRLNPGGVMLQWAQGYALHPSDLRMVVRTYQEA
                     FPAVSIWSTYKRGDYLLLGRAEPRPLDLGRLRTAYETIPGLRQDMARSGQRSPYALLA
                     DFVLAEADATRFGSLGDVHTDDLLQLEFSAPRSLHTDTAPLNSRILRSFRTTEFPPVV
                     GDGRERLDDPAVRYDLGMAYLTSELPSEAATQFERALRAAPNHVPALLELAAVQQRLN
                     MPIQAVESLERALKREPGNAGAYFRLSQLYVSQRAPARALEAAERAVALKPRDAAHRA
                     HLARLLHEAGRLDEAARHFQTALESSSKDPAILTGLGMTYLAQGRGAEALPVLEQAQA
                     LRPDDSFVLHQLGRACLAVNQHARATTMLGRAAVLTPQVATIHADLGRAYLSAGDLER
                     AASSLERAIAVDPAQPGLVDVLGTVYARLSGAP"
     gene            4011..4769
                     /locus_tag="VLF19_01490"
     CDS             4011..4769
                     /locus_tag="VLF19_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HSE91950.1"
                     /translation="MIGPFAVLAALLGLVIGSFVNVVVARLPAGRSLWGPHSACLACG
                     TPIAWHDNIPLLSFALLRGRCRTCGVAISPRYPIIEAMTGGLFVLAYATLGLDSDLVI
                     AFLLLPALVAITAIDLEHQIVPDKITLPGIVAGVIANVACQRVPWVDSIIGIVVGGGV
                     FLVIILASGGGMGGGDMKLGAMLGAFLGWKIALLSILVAVIVGGAVAVAFLASGTKGR
                     KEPIAFGPFLAGGGVVGLLWGERVITWYMSAFRV"
     gene            4742..5251
                     /locus_tag="VLF19_01495"
     CDS             4742..5251
                     /locus_tag="VLF19_01495"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HSE91951.1"
                     /translation="MVHERVQGLGQRGYSLAEIMVAVAVVGVLFAASAPSFISYWRAA
                     ATRAAARELTAALNHGRQLAIARNCSVRVMTNGAGSLRFFDPGVTCNPTLAPPGAPAD
                     GFWRGPGTDGMGWMRIANDIQLTGPGVDVAFTNLGAAVPGGVFTVRNPIDSQQLTVTV
                     AASGRITTP"
     gene            5248..5709
                     /locus_tag="VLF19_01500"
     CDS             5248..5709
                     /locus_tag="VLF19_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSE91952.1"
                     /translation="MTRHRHVIASTRDQRGFTMVEVLIATAIIGVALVAVMLGFGYAT
                     TGTEAGRQQTTAVFLAEQRVEAVRSIGVNVGAANYWTDPQIAAGVTQEAYGAIPNAPG
                     YRRVTTITDAPPPPPVNWKRIQVDVFYRPITERGSQPQERQVTVVSIVSRR"
     gene            5722..6408
                     /locus_tag="VLF19_01505"
     CDS             5722..6408
                     /locus_tag="VLF19_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSE91953.1"
                     /translation="MGTTRALVLGEGGFTLTELLVTLAIVGLVVGGLATMIVTGQQHY
                     VMGANQVEAQQNVRIAFDRLAREIREAGYNPTRNANWAAVVNVAGGAAMPTATAFMIQ
                     NDWNGDGAANPLAVCIPSNSAPPACAPGDTLRGEQVRYAIVGTQLTRQEIGIDAAAQV
                     LIAGVDTTGIAQPFDYRAFDGVTVPGAPANIAQVTVTLQAVSESNLGASATSGHVVVR
                     MNDTVRLRNK"
     gene            6431..7813
                     /locus_tag="VLF19_01510"
     CDS             6431..7813
                     /locus_tag="VLF19_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91954.1"
                     /translation="MNRITTALKDERGVALVMAMIMLVAMTGLMLAFLSVSAFEPQIS
                     QNLATASQARFVADAGLEWGYDRLASLPAGNVLDQKAQWDAFLAMPGGGTISGAAPMQ
                     IPGAGGPMSAAFGTFVVRVRNDTNDRDRMITGYCQTSAVVPCPPTLGGDTGGPTDDQN
                     AIVILTSTATVRGVTKTVTAAVRRLVPFGFNGAMSFPGRDAEIRYDGNTFDVSGKDWK
                     MPPPPPNPGSEPSVPDGVNPEVMGIVVSTEFPINEGVVESGLTAVQQDNVTGLAEGAG
                     AGEGANTINTDPGALMENVHSFIENIGQLAPSANLIEYTVARPADLSQPALTLPQTMG
                     SPTQPVVVHVKATDLDPLSEWTAVATQGGTVGYGVLIVEDGDLKVNGNFRWNGPVIVS
                     GKYVSVYFNGAGEQSIYGAVVINETSTDPTLEAWLRGNIKIRYSKEALDFAMNGLFGQ
                     RARMYSWREN"
     gene            8196..9239
                     /gene="pilM"
                     /locus_tag="VLF19_01515"
     CDS             8196..9239
                     /gene="pilM"
                     /locus_tag="VLF19_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HSE91955.1"
                     /translation="MAFLRRTRDAFGLDIGSSAVKAVQLRNGHGHRLAALGVVPLDPD
                     TISEGTIRNPEFVASAIREAVAMAGIKSRDAVIGVSGRELIIKKVQIPEVPARELRDA
                     VQLEAEHHIPFSIDEVFLDHHVVGMREGVMDLILVAVKKARVTEYTAVVEDAGFAPVV
                     VDVDSFALGNQFELNHPEALGEAVALIDIGASTMKTNVVRGGASVFARDIPFGGNNYT
                     QAIAQHLRIPVGQAEAAKLGKNVGVAWDAIVPALESVSRELSLEIQRTFDYFASTADS
                     ERIGKIVLAGGCSLLPGLTDYLSSTWGVGVEVAHPFERVDVGDFAETVAAAGPSLAVA
                     VGLGLRHAGDKEP"
     gene            9236..9793
                     /locus_tag="VLF19_01520"
     CDS             9236..9793
                     /locus_tag="VLF19_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HSE91956.1"
                     /translation="MIRINLGQDGDRRGGPSFSLGLPSFNVGMLFLAVYVLGVGGLGF
                     YWLTLHREEARLTAELDRARSENERLKVANGQAGNIKERVVEMQKRVASVHDLVKSQG
                     RPIQLLDAFVDTIPQDLWITALEEKNAVVKIVGTAYSSTAVSDLMSNLKRSGKFKDID
                     IAIARQDLVKSPRPVTFEVTCRFEG"
     gene            9796..10476
                     /gene="pilO"
                     /locus_tag="VLF19_01525"
     CDS             9796..10476
                     /gene="pilO"
                     /locus_tag="VLF19_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSE91957.1"
                     /translation="MALPAFFDPIATGPRWQKLLLGIMGLAVIAGVSYYLLISPAETR
                     VAALGGQLTLVQAELAQNRRIAADIARYRQELAQLERRMEAIRERLPNEKEMPALYRT
                     VSDAAYQAGLGVALFEPKAPKITDYYSELPIVVAAEGGYHDVGTFLGRVASLPRVVNL
                     TDWKLSGLTKGKGSLKADLMLATYMYRPPGSAPAPKPATAGAAPGVLPPQPPIATRPA
                     QPEPRVTR"
     gene            10483..11052
                     /locus_tag="VLF19_01530"
     CDS             10483..11052
                     /locus_tag="VLF19_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91958.1"
                     /translation="MDIASRLATTVGLATLLSACGGSPPALPPAPVAGSGATLPPAPG
                     GATLPPAPVAGSGVSLPPAPVVGSDAALPAASPSASVETVKLEFPAPSDVKWEARGRR
                     DPFEPLVAREGAAGTTVSSAKLTGIVRRGGTALALVETPDGLGYILKAGDTLGDGRVL
                     EIGQNTVVFSIVPRPGITDNRVVLRLAGE"
     gene            11079..>12842
                     /locus_tag="VLF19_01535"
     CDS             11079..>12842
                     /locus_tag="VLF19_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HSE91959.1"
                     /translation="MHAVLALVIGLLLFPTLALAAAAETTPPSDAARAKVSDVTVTER
                     SDSTRVHIRTTGAAKYRAEFIDSPWRLVIDLDDTVYAWRKTPLPIGKEPLKQVRGGQY
                     RKGVTRVVVEFTRKSPYAIREDADGLAIIVPTVHAEAPNDTASAAVAATPAPGPAPTP
                     IPPAQAPKAPAQVREPGVPAAAVREAPAPVRIAQAQAAPAPAGTGRPISLEFKDADVV
                     NLLRILAAESGRNVVIGEDVKGKMSISLRNVPWQLALDTILEARGLQKIEKDGVLRIV
                     STEQLTKEREAQARVNEAKIKGEAEARAKLADAQVKEAEAQTKRLAAQAAAKEQEAKG
                     PLREETVRLSYADPDEVAKTLQGILGIPAEGAKIQGPGYLGLPGPPNPELGVGYQGAP
                     PAGPVPAVTPVSVSQDVLARGLTIRAHKPTNTLFLRLYEADLERVKTIIREALDIPLP
                     QVKIEARMEILDRSALEQIGIQWGGATAQTPGNMTLVGQGFQTTQVGGQTVGFLPGIV
                     QGSQNVIDLVPANGGAGFGPGVLAQPSVIPPAIPPATGGFPISFQTGLPAGGNLINLP
                     ISALPNAGPLPAAGIAFGLVGS"
BASE COUNT         1850 a         4633 c         4471 g         1888 t
ORIGIN      
        1 gcctcgtcca cgccggccac cacctcgcgt acgatcgcta cagctatctc gcgtgcctgg
       61 gctgggcgct gctggctggc ggcggcatcg ccgcggcctg ggaagcccgc gcgcgggtgc
      121 ggccggcatt cgcgcgtgcc gccgtcgccg ccgcggcggt gggcgcgctc gggctcggcg
      181 tgatcacggt gtaccagatc cgcgtctggc acaacaccga gaccctctgg cggcacgccg
      241 tcgccgtcga cccggggtgc tcgatctgcc gggacaacct cggggtggcg ctgtacaacg
      301 ccaaccggat cgacgcggcc cgggcggagt tcgagacggc gctcgcgctg cgccccgatc
      361 gggccagggc gcgcctcaac ctgggcgtcg cgctggtcgc gctcggcgag cccgagcgcg
      421 ccgaggccga gttcgcgcgc gtggtctcgg cgcatcccac gtccgccagc gcgctgaacg
      481 cgctcggcgt ggcgctgatc aggcagcggc gacccgaggc cgcactgacg tacctggagc
      541 gagccgtcgc gctcgccgag ggcgacccgt acgcgcgcac tcacctcgcc gtcgccctca
      601 ccgcggccgg acggccgggt gacgccctcg gccactatcg acgcgcgatc gagctgttgc
      661 ccgatgccgg cctcccgcgg ctgggtctgg cccgcgcgca cctcgcgctc ggcgacctgc
      721 gcggcgcgcg cgagcactac gaggcgctga aaaccatcga cgcgcggctg gccgggcagc
      781 tcgcgctcga gatgcacgcg agcatccagg ggggagccgg tgaacgcttc tgatcggcaa
      841 cgcgggcgcg tcctgaccgg ggttctcgtg tgcttcttct tctccggctt cacgggcctg
      901 gtgtacgagg tgctgtggat ccggatgctc gggctcgtct tcggacagac ggtgttcgcg
      961 atcaccacgg tcctcgccgc cttcatggga gggctcggtc tcggcagctt cctgttcggg
     1021 cgcgtcgtcg accgcgacca gcggccgctg cggacctacg ggctcatcga gatcggcatc
     1081 ggggtctcct gcctcctcgt gccggtcgtg ctgccctggg tgtccacgct ctacctcggc
     1141 ctgcaccgag cgctcgggct ctcgtacttc ggcttcagcc tgacccagtt cgtgctcgtg
     1201 ctcgccgtgc tgctcgtgcc gacgacgctg atgggcggca cgctgcccgt cctgagccgc
     1261 ttcttcgccc gcgacgcggt cacgctgagc cgccgcgtcg gcctgctcta cgcactcaac
     1321 actttcggcg ccgtcgccgg caccgccgcg gcggggtacg gattgctgcc cctgttcggc
     1381 gtgcgcggca cgctcctcct cgcggcgacg ctgacgatcg gtgtgggcgc gctggcgatc
     1441 gtcttctcgg cacatatgga ccgcctggcc tcggacgcgc cggcacgcga gaccgagccg
     1501 gcgcccgatc cccagggcca gcccgccggc gtcggtcggc tcgcgggcgt ggtcaccctc
     1561 ggcctcggcg tctcgggcgc gtcctccatg atctacgaga tcggctggag ccgggcgctg
     1621 gccctggtcc tcggcagctc gacctacgcc ttcacggcga tgctggtggc cttcctggtc
     1681 ggtatcgcgg ggggcagcgc gctcttctcg cggctgcggg cgtcacgcgc ggcgacggcg
     1741 ggcacgttcg cgctgatcca gctcggcatc gcgacgtccg cgctcctcat ccttcccgcc
     1801 ttcgaatgga tgccccgggc tctgctgcgg gccttgaccc tctcgctcga cccggggttc
     1861 gtgatggccg cgcagatcgt catgtcgatc gccgcgatga tcgtgccgac gctgctgatc
     1921 ggcgcgagct ttccgtgcgc gatcgatctg gtcgcgcggc gggtggagcg ggcgggcttc
     1981 gatgtggggc gtctctactc cgtcaacacg ctcggagcca tcgtcggcac ggtcctcgcg
     2041 ggattcctgc tgatcccggc gctcggggca cagaccaccg tcaaggtcgc ggtggcgctg
     2101 aacgccctga ccggcctcgc catcgccgtc acgggcgggc aggccgtgcg ccgcccgcag
     2161 tgggcgggga tcggagccct ggccgccgtc accgcggccg cgatcggctg gatcccggcg
     2221 tggaacgccg cggcgatgac gaccggcgtc gcggtctacg gccacgtcta ccggagtgcg
     2281 ccatccctgg cgcggggccg cgaggtcctc ctctacgagg acgggctgac ggcgaccgtg
     2341 agcgtccacc gggccggcgg caacatctcg ctccgcgtga acggcaagac cgacgccagc
     2401 aacggggccg acatgcacac gcagaccatg tcggcgcacc tgccgctgct cgcgcacccg
     2461 gaccccaagt ccgtgctcgt catcggcctc gcgagcgggg tcacggtggg cgccgcggcg
     2521 cagcacccgg tcgagcgcat cgacgtggtg gagatcgagc ccgcgatgcg gcgcgccgcc
     2581 cagttcttcg cggacgagaa ccgccgcgtg ctcgacgacc cgcgcgtgcg ggtgatcttc
     2641 gccgatggcc gcaacttcct gctggcggcg acgcaacggt acgacgtcat catctcggag
     2701 ccgtcgaacc cctggatccg cggcgtggcg acgctgttca cccgcgagtt ctacacgctc
     2761 gcccggtctc gcctcaaccc gggcggcgtc atgctccagt gggcgcaggg ctacgcgcta
     2821 catccgtcgg acctccggat ggtcgtcagg acgtaccagg aggcgttccc ggcggtcagc
     2881 atctggtcca cgtacaagcg cggcgactac ctgctgctcg gccgtgccga gccgaggccg
     2941 ctcgacctcg gccggctccg gaccgcgtac gagacgattc cgggcctccg ccaggacatg
     3001 gcgcgctccg gtcaacgctc gccctacgcg ctcctggccg atttcgtcct cgccgaggcc
     3061 gacgccacgc gcttcggctc gctcggcgac gtccacacgg acgacctgct ccagctcgag
     3121 ttctccgcac cccgcagcct tcacacggac acggccccgc tcaattcccg gatcctgagg
     3181 tcgttccgga cgacggaatt tcccccggtg gtcggcgacg ggcgagagcg gctcgacgat
     3241 ccggcagtcc gctacgatct cgggatggcg tacctcacga gcgagctgcc ctcggaggcg
     3301 gccacccagt tcgagcgcgc gctgcgcgcg gcgcccaacc acgttcccgc gctcctcgag
     3361 ctggccgcgg tgcagcagcg actgaacatg ccgatccagg ccgtcgagag cctggagcgc
     3421 gccctcaagc gagagccggg caacgccggc gcctacttcc gcctctcgca gctgtacgtc
     3481 tcccagcgcg cgcccgcgcg cgcgctggag gccgcggagc gcgccgtcgc gctgaagccc
     3541 cgggacgcgg cccaccgcgc ccacctcgcc cgcctgctgc acgaggccgg gcggctcgac
     3601 gaggcggcgc gccacttcca gacggcgctg gagagctctt cgaaggatcc cgcgatcctg
     3661 accgggctcg gcatgacgta cctggcccag gggcgcggcg cggaggcctt gcccgtcctc
     3721 gagcaggcgc aggcgctccg gccggacgac agcttcgtcc tccaccagct cgggcgcgcc
     3781 tgtctcgcgg tgaaccagca cgcgcgcgcg acgacgatgc tcgggcgcgc ggccgtcctg
     3841 accccgcagg tcgcgacgat ccacgcggac ctcgggcgcg cgtatctttc cgcgggcgac
     3901 ctcgagcgcg ccgcgtcgtc gctcgagcgc gcgatcgccg tcgaccccgc gcagccgggg
     3961 ctggtggacg tcctcggcac ggtctatgcg cgactgagcg gggcgccgtg atgatcggcc
     4021 cgttcgcggt cctcgccgcg ctgctcggcc tcgtcatcgg cagcttcgtg aacgtcgtcg
     4081 tcgcgcggct gccggccggc cgcagcctct ggggaccgca ctcggcctgc ctggcgtgcg
     4141 ggacaccgat cgcgtggcac gacaacatcc cgctgctctc gttcgctctc ctgcgcggcc
     4201 gatgccgcac gtgcggcgtc gcgatctccc cgcgttatcc gatcatcgag gcgatgacgg
     4261 gtggactgtt cgttctcgca tacgcgacgc tcgggctcga ctccgacctc gtcatcgcgt
     4321 tcctgctgct gcccgccctg gtcgcgatca ccgccatcga cctcgagcat cagatcgtcc
     4381 ccgacaagat cacgctgccc ggcatcgtgg caggcgtcat tgccaacgtg gcatgccagc
     4441 gtgttccctg ggtcgactcg atcatcggaa tcgtcgtcgg tggtggtgtc tttctggtca
     4501 tcatcctggc gagcggcggc ggcatgggcg gcggcgacat gaagctcggc gccatgctcg
     4561 gcgcgttcct gggctggaaa atcgcgctgt tgtcgattct cgtggcggtc atcgtcggtg
     4621 gcgccgtggc ggtcgcgttc ctggcgtcag gaacaaaggg tcgcaaggag ccgatcgcgt
     4681 ttggtccgtt ccttgctggt gggggagtgg tcggactcct ctggggcgag agggtgatca
     4741 cttggtacat gagcgcgttc agggtctagg gcagcggggc tattcgctcg cggagatcat
     4801 ggtcgccgtc gccgtcgtcg gcgtcctctt cgccgcgtcg gccccgagct tcatcagcta
     4861 ctggcgcgcc gcggcgacga gggcggccgc gcgtgagctc acggcggcgc tgaaccacgg
     4921 ccgccagctc gcgatcgccc gtaactgctc cgtgcgcgtg atgacgaacg gcgccgggag
     4981 ccttcgcttc ttcgatcccg gcgtcacctg caatccgacg ctggctcctc ccggcgcccc
     5041 tgccgacggc ttctggcgcg gcccggggac cgacggcatg ggctggatga ggatcgcgaa
     5101 cgacatccag ctcacggggc ccggcgtgga cgtcgcgttc acgaatctgg gggcggccgt
     5161 tccagggggg gtgttcacgg tgcgtaaccc gatcgacagc cagcagctca ccgtcacggt
     5221 cgcggcgtcg ggccgcatca ccacgccatg acccgccatc ggcacgtcat cgcctccacc
     5281 cgcgatcagc gcggcttcac catggtggaa gtgctcatag ccacggccat catcggcgtg
     5341 gccctcgtcg ccgtcatgct gggcttcggc tacgcgacca ccggcaccga ggccggccgg
     5401 cagcagacca ccgcggtctt cctcgccgag cagcgggtcg aggccgtgcg gtccatcggg
     5461 gtcaacgtgg gtgccgcgaa ctactggacc gatccccaga tcgcggccgg cgtcacccag
     5521 gaagcgtacg gcgcgatccc gaacgcgccc gggtatcgcc gggtcaccac catcacggac
     5581 gcgccgccgc cgccgccggt caactggaag cgcatccagg tcgacgtgtt ctaccggccg
     5641 atcaccgagc gcggatccca gccgcaggag cgccaggtga cggtcgtctc gatcgtcagc
     5701 cgacgctgac aggagggcca cgtgggcacg acgcgggcgc tcgtcctcgg cgagggaggc
     5761 ttcacgctca cggagctgct cgtcaccctg gccatcgtcg gcctcgtggt gggcggtctc
     5821 gccacgatga tcgtgacggg gcagcaacac tacgtgatgg gagccaatca ggtcgaggcc
     5881 cagcagaacg tccggatcgc cttcgaccgc ctcgcgcgcg agatccgcga ggccggctac
     5941 aacccgacgc ggaacgcgaa ctgggcggcc gtcgtcaacg tggccggagg cgcggccatg
     6001 cccacggcga ccgccttcat gatccagaac gactggaacg gcgacggcgc ggccaacccc
     6061 ctcgccgtct gcatcccctc gaactctgcg ccgccggcct gtgcgcccgg cgacacgctg
     6121 cgcggcgagc aggtgcgcta cgccatcgtc ggcacgcagc tcacccgcca ggagatcggg
     6181 atcgacgcgg ccgcacaggt gctgatcgcc ggcgtcgaca ccacggggat cgcccagccc
     6241 tttgactatc gagccttcga cggcgtcacc gtcccgggcg cgccggcgaa catcgcgcag
     6301 gtcacggtga cgctgcaggc cgtgtcggag agcaacctgg gggcgtcggc gacctcgggg
     6361 cacgtggtcg tgcggatgaa cgacaccgtc cgcctcagga acaagtgacc ggggcggcgt
     6421 ggaggggacc atgaacagga tcacgacagc gctcaaggac gagcgcggcg tggcgctcgt
     6481 gatggcgatg atcatgctag tcgccatgac gggcctcatg ctggcgttcc tctcggtgag
     6541 cgccttcgag ccgcagatct cgcagaacct ggcgacggcc tcgcaggcgc gcttcgtcgc
     6601 cgacgcgggc ctcgagtggg gctacgaccg gctcgcgagc cttcccgccg gcaacgtgct
     6661 ggaccagaag gcccagtggg acgcgttcct ggcgatgccg gggggaggca cgatctccgg
     6721 cgccgccccg atgcagatcc cgggggcggg cggtccgatg agcgcggcct tcggcacctt
     6781 cgtggtgcgt gtccggaacg acacgaacga ccgcgaccgg atgatcaccg gctactgcca
     6841 gacctccgcg gtggtgccgt gcccgccgac gctggggggc gacacgggcg ggccgaccga
     6901 cgaccagaac gccatcgtga tcctcacgtc gaccgccacg gtgcggggcg tgaccaagac
     6961 cgtcaccgcg gccgtcaggc ggctcgtgcc gttcggcttc aacggcgcca tgtcgttccc
     7021 gggcagggat gcggagatcc gttacgacgg caacaccttc gacgtgtccg gcaaggactg
     7081 gaagatgccg cccccgcccc ccaaccccgg ttcggagcca tcggtgccag acggggtgaa
     7141 ccccgaggtc atgggcatcg tcgtatcgac tgaattcccg atcaacgagg gcgtggtcga
     7201 gtcggggctg acggccgtcc agcaggacaa tgtcaccggg ctggccgagg gcgccggagc
     7261 cggcgagggc gccaacacga tcaacaccga tccgggtgcc ctgatggaga acgtccacag
     7321 cttcatcgag aacatcgggc agctggcgcc cagcgccaac ctcatcgagt acacggtcgc
     7381 ccgaccggcc gatctgtcgc agccagcgct cacattgccc caaacgatgg gcagcccgac
     7441 gcagcccgtg gtcgtgcacg tcaaggcgac cgatctcgac ccgctgtccg aatggacagc
     7501 ggtggcaacc cagggcggca ccgtcggcta cggagtcctg atcgtcgagg acggtgacct
     7561 caaggtcaac ggcaacttcc gttggaacgg ccccgtcatc gtctcgggaa agtatgtcag
     7621 cgtctacttc aacggtgctg gcgagcaatc catctacggc gcggtggtca tcaacgagac
     7681 gtccaccgac cccactctcg aggcctggct caggggcaac atcaagatcc ggtacagcaa
     7741 ggaagccctc gacttcgcga tgaacgggct cttcggccag cgggcgcgga tgtacagctg
     7801 gcgggagaac tgacgatgcg ccgggcgtgg gcggccgccg gcgtcaccct ctgcgggagc
     7861 tgcgggcaat ccccggctcc gcccgcgacc gtagccgtga accccctgac cgcctcggcg
     7921 cgggaggcag ccacgccccg gtcccggcgc ggcgaccggg cagcggcagt ggtcaaggac
     7981 cgcgaggccg cggcgctccg cggagtggcg aaagtgggac cgataggttc gatgtcgccg
     8041 cgagactttc ctccgcgtgc cggcggctga acggccccga gcccatggac cgggggtcgg
     8101 tcctggggcc gaccccattg cgcaggggtg acggaaaaga taggggcggg gtccacccgc
     8161 cccctagact gaagtaggaa agtgagcgtt tgagcatggc attccttcgt cgtaccaggg
     8221 acgcgttcgg gctcgacatc ggatccagcg cggtgaaggc cgtccagctc cggaacggcc
     8281 acggccaccg gctcgcggcg ctcggcgtgg tgcccctcga tcccgacacg atctcggagg
     8341 gcaccatcag gaaccccgaa ttcgtcgcat ccgccatcag ggaggcggtg gcgatggcgg
     8401 gcatcaagag ccgcgacgcc gtcatcgggg tgtcgggccg cgagctcatc atcaagaagg
     8461 tccagatccc cgaggtgccg gccagggagc tgcgcgacgc cgttcagctc gaggccgagc
     8521 atcacatccc gttctcgatc gacgaggtgt tcctcgacca tcacgtcgtg ggcatgcgcg
     8581 agggcgtgat ggacctcatc ctcgtcgcgg tcaagaaggc gagggtgacc gagtacacgg
     8641 ccgtggtcga ggacgcgggc ttcgccccgg tcgtcgtcga cgtggacagc ttcgcgctcg
     8701 gcaaccagtt cgagctgaat caccccgagg cgctgggcga ggcggtggcg ctgatcgaca
     8761 tcggcgcctc gacgatgaag accaacgtcg ttcgcggcgg cgcctcggtc ttcgcgcgcg
     8821 acatcccctt cggcggcaac aactacaccc aggcgatcgc ccagcacctg cgcatccccg
     8881 tcgggcaggc ggaggccgcg aagctgggca agaacgtggg ggtcgcgtgg gatgccatcg
     8941 tgccggcgct cgagtccgtg tcgcgcgagc tgtcgctcga gatccagcgc accttcgact
     9001 acttcgcgtc gacggcggac tcggagcgca tcggcaagat cgtgctggcc ggcggttgct
     9061 cgctgctgcc cggcctgacc gactatctgt cgtccacctg gggtgtcggg gtggaagtcg
     9121 cacacccctt cgagcgtgtc gacgtcggtg acttcgccga gacggtcgcg gcggccgggc
     9181 cctcgctggc cgtcgccgtg ggcctgggcc ttcgtcacgc aggagacaaa gagccttgat
     9241 caggatcaat ctcggacagg atggtgatcg ccgcggaggt ccctcgttca gcctcggcct
     9301 gccgagcttc aacgtcggca tgctgttcct ggccgtctac gtgctcggcg tgggcgggct
     9361 cggcttctac tggctgaccc tccaccgcga ggaggcgcgt ctgaccgccg agctggacag
     9421 ggcgcggagc gagaacgagc ggctcaaggt cgccaacggc caggccggca acatcaagga
     9481 acgggtcgtc gagatgcaga agcgggtcgc gtccgtgcac gacctcgtga agagccaggg
     9541 ccggcccatc cagctgctcg acgcgttcgt cgacacgatt cctcaagacc tctggatcac
     9601 ggccctggag gagaagaacg cggtggtcaa gatcgtgggc acggcgtact cgtcgacggc
     9661 cgtgtccgac ctcatgtcga atctcaagcg ctccggcaag ttcaaggaca tcgacatcgc
     9721 gatcgcgcgc caggatctcg tgaagagccc gcgcccggtg accttcgagg tcacctgccg
     9781 cttcgagggt tgaccatggc acttccagcc ttcttcgacc cgatcgccac tgggccccgc
     9841 tggcagaagc tcctcctcgg catcatgggg ctcgccgtca tcgcgggcgt ctcctactac
     9901 ctgctgatct cgccggccga gacgcgggtc gccgcgctcg gtggccagct caccctcgtg
     9961 caggccgagc tggcgcagaa ccggcgcatc gccgccgaca tcgcgcgcta ccggcaggag
    10021 ctcgcccagc tggagcgccg catggaggcg atccgggagc ggctgcccaa cgaaaaggag
    10081 atgccggcgc tctaccggac cgtctccgac gcggcgtacc aggcgggcct gggcgtcgcc
    10141 ctcttcgagc cgaaggcgcc gaagatcacc gactactaca gcgagctccc gatcgtcgtc
    10201 gccgccgagg gcggctacca cgacgtgggg acgttcctcg ggcgggtggc gagtctgccg
    10261 cgcgtcgtga acctgaccga ctggaagctg tcggggctca ccaagggcaa ggggtcgctg
    10321 aaggcggacc tgatgctcgc gacgtacatg taccggcccc cggggtcggc tcccgcgccg
    10381 aagccagcca cggccggcgc ggcacccggc gtccttccgc cccagccacc cattgcgacc
    10441 cggccggctc agccggagcc gcgagtgacg aggtaatcca cgatggacat cgcgtcacgt
    10501 ctggccacga ccgtcggtct cgccaccctg ctgagcgcat gcggcggctc cccgcccgca
    10561 ctgccgcccg cgccggtggc cggctccggc gccaccttgc cgcccgcacc gggcggcgcc
    10621 accctgccgc ccgcgcccgt ggccggctcc ggtgtcagct tgccgcccgc gcccgtggtc
    10681 ggctcggacg cggccctccc ggcagcgtcg ccctccgcgt cggtcgagac cgtcaagctc
    10741 gagttcccgg cgccgtccga cgtgaagtgg gaggcgagag gtcgtcgcga cccgttcgag
    10801 ccgctcgtgg cccgcgaggg cgcggcgggg acgaccgtgt cctcggcaaa gctgacggga
    10861 atcgtgagga gaggcggcac ggccctcgcg ctggtcgaga cgcccgacgg gctgggctac
    10921 atcctgaagg ccggcgacac gctcggggac gggcgagtgc tcgagatagg acagaacacg
    10981 gtcgtcttca gcatcgtccc gcgcccgggc ataaccgaca accgcgtagt cctgaggctg
    11041 gcgggtgagt gatgactcca cgacggggta aaccggtggt gcacgccgtt ctcgcgctgg
    11101 tgatcggtct cctgctgttc cccacgctgg ctctggcggc ggcggcggag accacgccgc
    11161 cgtccgacgc cgcacgggcg aaggtcagcg acgtcaccgt caccgagcgg tccgactcga
    11221 cccgcgttca catccggacg acgggcgctg ccaagtaccg cgccgagttc atcgattccc
    11281 cgtggcgcct cgtcatcgac ctcgacgata cggtctacgc ctggcgcaag acgccgctcc
    11341 cgatcggcaa ggagccgctg aagcaggtcc gcggcggcca gtaccgcaag ggcgtgacgc
    11401 gcgtggtcgt ggagttcacg cgcaagtccc cctacgcgat tcgcgaggac gccgacggcc
    11461 tggcgatcat cgtcccgacc gtccacgccg aggcgcccaa cgacaccgcg agcgccgcgg
    11521 tcgcggcgac gccggctccc gggccggcac ccacgcccat cccgccggcg caggctccga
    11581 aggcccccgc ccaggtgcgc gagccgggcg tcccggccgc cgcggtgcgc gaggcgcctg
    11641 cgccggtccg catcgcacag gcgcaggcgg cacccgcccc ggccggcacc ggtcgtccca
    11701 tctcgctcga gttcaaggac gccgacgtcg tcaacctcct gcgcatcctc gcagcggaga
    11761 gcggccgcaa cgtcgtcatc ggcgaagacg tgaaggggaa gatgtcgatc tccctccgga
    11821 acgtcccgtg gcagctggcg ctcgacacga tcctcgaggc gcgcggcctc cagaagatcg
    11881 agaaggacgg ggtgctgcgc atcgtctcca cggagcagct gaccaaggag cgcgaggcgc
    11941 aggccagggt caacgaggcc aagatcaagg gcgaggccga ggcacgcgcg aagctcgccg
    12001 acgcgcaggt gaaggaggct gaggcccaga ccaagcggct ggctgcccag gctgcagcca
    12061 aggagcagga ggccaagggg ccgctgcgtg aggagacggt ccgcctctcg tacgcggacc
    12121 ccgacgaggt cgcgaagacg ctccagggta tcctcggcat ccctgccgag ggcgcgaaga
    12181 tccaggggcc cggctacctc gggctgcccg ggccgccgaa ccccgagctc ggcgtgggct
    12241 accagggcgc gcccccggcc ggtccggttc ctgccgtgac gcccgtgtcg gtctcacagg
    12301 acgtgctggc cagggggctg acgatccgcg cgcacaagcc gacgaacacg ctgttcctgc
    12361 gcctctacga ggccgatctc gagcgcgtga agacgatcat ccgcgaggcg ctcgacatcc
    12421 cgctgccgca ggtcaagatc gaggcgcgga tggagatcct cgaccgcagc gcgctcgagc
    12481 agatcggcat ccagtggggc ggcgccaccg cgcagacgcc gggcaacatg accctggtcg
    12541 gccagggctt tcagacgacg caggtcggcg ggcagacggt gggtttcctg cccggcatcg
    12601 tccagggctc gcagaacgtc atcgacctcg tcccggccaa cgggggcgcc ggcttcgggc
    12661 ccggggtcct cgcgcagccc agcgtgatac cgccggcgat cccgccggcg acgggcggct
    12721 tcccgatcag cttccagacg ggcttgccgg ccggcggcaa cctcatcaac ctgccgatca
    12781 gcgccctgcc caacgcgggt cccctgccgg cggcgggcat cgccttcggc ctcgtcggct
    12841 cc
//
LOCUS       DATIAZ010000046        10652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_273877, whole genome shotgun sequence.
ACCESSION   DATIAZ010000046 DATIAZ010000000
VERSION     DATIAZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273877"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VLF19_01540"
     CDS             complement(<1..603)
                     /locus_tag="VLF19_01540"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HSE91960.1"
                     /translation="MTAGSGAPLTPTPLGEHLSRARGQHAADLGAIVGCLAEAGVTIA
                     RELGRAALVGRLGTTGTTNVQGETVKKLDLWANDVVVQALGATGRVATLVSEEMEDPL
                     RRPVRGSAPWTVCFDPVDGSSNLDVNGVVGTIFSVRPGDAPSQPGTAQVAAGYIMYGP
                     STVLVLATDDGVDAFTLDVTIGRFLHSLAKIRIPPSGHIYS"
     gene            complement(600..1352)
                     /gene="pgl"
                     /locus_tag="VLF19_01545"
     CDS             complement(600..1352)
                     /gene="pgl"
                     /locus_tag="VLF19_01545"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSE91961.1"
                     /translation="MSTVRSFDDADAVAQAGAEEFVTLAAAAAGARGRFMVALAGGST
                     PRRMYRLLAEPAWRDRVAWDRVEVFWGDERAVPPDHADSNYHMAATALLERVPVPSGR
                     VHRIRAEAADLADAAREYQAEIARVFGVPEDGPPPAFDLILLGMGADGHTASLFPGTT
                     GLDERRRWVVSHAVPSMRTERVTLTLPIINRAREIRFLVTGEDKAGRLREALTPPRGT
                     PLPVQRVAPEAGRLVWLADRAAAGTPAPVARS"
     gene            complement(1369..2355)
                     /gene="gnd"
                     /locus_tag="VLF19_01550"
     CDS             complement(1369..2355)
                     /gene="gnd"
                     /locus_tag="VLF19_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HSE91962.1"
                     /translation="MQIGMVGLGRMGGNMARRLMRGGHEVVGFATDAKAVQDLAKDGA
                     AGTTSLDDLVKKLRAPRVAWVMVPAGAVTEQVVNDLAARMERGDTIVDGGNSMFKDDV
                     RRAKALLPRGIHYVDAGTSGGVWGLERGYCLMVGGETEVIKRLTPIFTTLAPGRGNIG
                     RTPGREKVSGGTAEDGWLHCGPVGAGHFVKMIHNGIEYGIMQAYAEGFDILKNADSSS
                     MPADQRYDLNLPDIAELWRRGSVVSSWLLDLTAQALVEHPTLGKYTGVVQDSGEGRWT
                     VQQAVETAVPADVLTASLYVRFRSRQQNTFAEKVLSAMREKFGGHVEPPAGG"
     gene            complement(2498..4564)
                     /gene="tkt"
                     /locus_tag="VLF19_01555"
     CDS             complement(2498..4564)
                     /gene="tkt"
                     /locus_tag="VLF19_01555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSE91963.1"
                     /translation="MSADLDQLSINTIRTLSMDAVQQANSGHPGTAMAMAPVIYTLWQ
                     RQLRFDPDDPIWGNRDRFVLSMGHASMLLYSMLHLAGVKAVNPQYERLGSLSVTLDDI
                     KRFRQLDSKCAGHPEYRWTSGVETTTGPLGAGVATSVGMAIAARWMAARFNRPDFPMF
                     DYDVYALGGDGCFMEGISGEAASLAGHLRLGNLCWIYDNNRITIEGSTALAFTEDVAT
                     RFLGYGWNVLRVGDANDISMLERALATFKRTPDRPTLIIVDSHIAWGSPHKQDTHAAH
                     GEPLGEEEIRLTKRAYGWPEDAKFLVPDGVREHFGAGIGARGKAARMAWVEMFGRYRD
                     KYPELADHLLRMQRRQLPEGWDKGLDPFPADPKGVAGRDASAKVLNTLAKNVPWLVGG
                     SADLAPSTKTRLTFEGAGDFAAETPHGRNLHFGVREHAMGAVLNGLALSKVRAYGSGF
                     LIFSDYMRAPIRLSALMELPVVYIFTHDSIGVGEDGPTHQPVEQVASLRAIPGLITFR
                     PCDANEVLEAWRYTMTLRHEPVAMILSRQALPTIDRAKHGDARGTAKGAYVLADAPDG
                     RPDVILMATGSEVALCLAAREKLAAEGVRARVVSMPSWELFDRQPEDYRAAVLPPGMT
                     ARVAVEQASTLGWARWVGSEGEIIGMRTFGASAPLKELQSKFGFTPDAVVAAAKRTVA
                     RARGEK"
     gene            complement(4561..5253)
                     /gene="rpiA"
                     /locus_tag="VLF19_01560"
     CDS             complement(4561..5253)
                     /gene="rpiA"
                     /locus_tag="VLF19_01560"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HSE91964.1"
                     /translation="MAIVSEQLLGALADRALSFVREDTIVGLGSGRAATAFVKSLGAR
                     VGQGLRITGVPTSDATAALARSVGIPLVGLDAGDLDIAVDGADEVAPNLDLVKGWGGA
                     LVRERIVAMAARRRVILVDSEKLVDRLGRRGRVPVEVLPFATAFCQRKLSALGCRPAL
                     RMADGRPFVSDNWNVILDCALDPIDDPRALERAIRAIPGVIDTGLFLDVADTVLVWDG
                     GVVRALERKEPR"
     gene            complement(5396..7387)
                     /locus_tag="VLF19_01565"
     CDS             complement(5396..7387)
                     /locus_tag="VLF19_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSE91965.1"
                     /translation="MPLAPVDVGREVTGDFELAAGREWLCANGIGGFASGTIAGALTR
                     RYHGLLVAALSPPLGRTLMAVKVDETVTYGGVTRALGANAWASGIVEPAGHRHLVRFA
                     LDGTTPVWTYACADALVEKRIFMEPGANTTYVRWRVLRAQAPLALEAAVLVDHRDYHG
                     ATRGGWPMRVEVVPKGLRVTAFDGARPLLLLADGAVIAPEHEWYYGFHLAREQDRGLE
                     FLEDHLRAGVLRATLPPGGEVTLVLSAEGAPSVDGAAAWDRREAWEADLLDRWRRAQP
                     TAPAAPAWIAQLVLAADQFVARRPLADDPDGMTVIAGYHWFGDWGRDTMIALPGLALT
                     TGRPEIARRILTTFARFVDRGMLPNRFPDAGEAPEYNTVDATLWYVEAVRAYHEATGD
                     DVLIGELFPTLETIVGAHRGGTRYGIGEDPADGLLRAGEPGVQLTWMDAKVGDWVVTP
                     RAGKPVEINALWYNALRVMAAFARRLGRRDDPWTAVADRVERSFARFWCERTGCCYDV
                     IDGPGGDDDALRPNQVFAVSLPASPLPPERQRRVVEVCARHLLTPMGLRSLAPGHPAY
                     RGRYEGGVRERDGAYHQGLVWSWLLGPFALAWRRVFDDAAGARDLLAPLAHHLGEYGL
                     GSIAEIFDGDPPHTPRGCIAQAWSVAETLRAWHALTEVG"
     gene            complement(7488..8294)
                     /locus_tag="VLF19_01570"
     CDS             complement(7488..8294)
                     /locus_tag="VLF19_01570"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSE91966.1"
                     /translation="MNDQSLKDQRALVTGAGSGIGAAIAMALGTAGAAVVVNYSRNRD
                     AALRVVAEIKDTGSDAMAVAADVASEADVEAMFERVQSAWGGIDIVVSNAGIQRDAAV
                     TEMTLGQWNEVIAVNLTGAFLAARAATRAMIRQGIRAGVSRAAGKIVFVSSVHEIIPW
                     AGHANYAASKGGVKLFMQSLAQELAPRRIRVNAVAPGAIQTPINRSAWETPEARAALL
                     RLIPYGRIGVPEDVGRVVAWLVSDAADYVHGQTLVVDGGMTLYPEFAHGG"
     gene            complement(8380..9177)
                     /locus_tag="VLF19_01575"
     CDS             complement(8380..9177)
                     /locus_tag="VLF19_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSE91967.1"
                     /translation="MPQPLTHRRIALLGGVYSNYLALEAALADARGRGAEAVYCLGDL
                     GGFGPHPDRVFPLLHDAAVACVQGNYDVSIAAGAADCGCGYTDPRDNDFAAVSYRYTL
                     ARTSSEHRRWLGALPPALRLTLGAWRLLLCHGSPRQVNEFLWESATPVGLLERFCDRY
                     DADVLCVTHTGLKWHRALPSGRHAVNVGALGRPENDGSPAVWYTLLTAGDDLEVDFVP
                     VHYDHEALAREMEREGLPPEFVETIRTGWWTCCLESLPAKERARGRW"
     gene            complement(9162..10055)
                     /locus_tag="VLF19_01580"
     CDS             complement(9162..10055)
                     /locus_tag="VLF19_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSE91968.1"
                     /translation="MTPRTRGAAPAPAAPTPWVALHDLRSVWLQVTGTLCNLACRHCF
                     NASGPRAPWLGSLDATAIRAAIAEAEGLGVKELYFTGGEPFLHPDLLALLETALAVAP
                     TTVLTNGTLVDDLGADRLAAIAASSAYSLEVRVSVEADTAEANDRVRGRGSFARALAA
                     VRRLDARGVPPIVTATKIVASRAGPTLYERLRACLLAAGVRRPRVKILPVLPLGRCAW
                     RADERCLTADDLEAFDVSRLPCAETRVVADGGVYACPILAGLPGARLSDGRLADALGP
                     ASLYHPVCVTCHATGLACRNH"
     gene            complement(10052..>10652)
                     /locus_tag="VLF19_01585"
     CDS             complement(10052..>10652)
                     /locus_tag="VLF19_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSE91969.1"
                     /translation="GRVPDETARHEYYRVIARESERLTRLIDNVLDFSRIEGGRRTYE
                     RVPTAVEPLVREVLETFAYPLEQHGFKVDVRVVPDVPEVPMDADAVGQALANLIDNAI
                     KYSADEKSLTIEARVAGDCLAITVADEGIGIPAEEHGRIFDKFYRVGRSDTQGRRGSG
                     VGLALVRHVAEAHGGRVTVESRPGEGSRFTMWLPVEVGR"
BASE COUNT         1475 a         3886 c         3846 g         1445 t
ORIGIN      
        1 gctgtagatg tggccactcg gcgggatgcg gatctttgcc agcgagtgca gaaacctccc
       61 gatggtcacg tcgagcgtga aggcgtccac gccgtcatcg gtcgcgagca cgaggaccgt
      121 cgaggggccg tacatgatgt agccggctgc gacctgcgcc gtccccggct gcgacggcgc
      181 gtcccccggc cggacgctga agatggtgcc gaccaccccg ttgacgtcga ggttcgagga
      241 gccgtcgacg ggatcgaagc agacggtcca cggcgcgctc cctcgcaccg ggcgtcgcag
      301 cgggtcttcc atctcttcgg agacgagcgt cgccacgcgg cccgtggcgc cgagcgcctg
      361 gaccacgacg tcgttggccc acaggtcgag cttcttgacc gtctcgccct ggacgttcgt
      421 cgtccccgtc gtcccgaggc ggccgaccag cgcggcacgg ccgagctcgc gcgcgatcgt
      481 caccccggcc tcggcgaggc agcccacgat cgcgcccagg tcggcagcgt gctggccgcg
      541 cgcgcggctc aggtgctcgc cgagcggcgt cggcgtcagg ggcgcgccgc tcccggccgt
      601 catgagcgcg ccaccggtgc aggcgtcccc gccgccgcgc ggtcggcgag ccagacaagc
      661 cggcccgcct cgggcgccac ccgctggacg ggcagcgggg tgcctctcgg cggagtcagc
      721 gcttcgcgca gcctgcctgc cttgtcctcg ccggtgacga ggaagcggat ctcgcgcgca
      781 cggttgatga tcggcagcgt cagcgtcacg cgctcggtgc gcatcgatgg tacggcgtgg
      841 ctcacgaccc agcggcggcg ctcgtcgagg ccggtcgtcc cgggaaagag cgaggccgtg
      901 tggccgtcgg cgcccatgcc gagcaggatc agatcgaacg ccggtggcgg gccatcctcg
      961 gggacgccga agacgcgcgc gatctccgcc tggtactcgc gcgcggcgtc ggccaggtcg
     1021 gcggcctccg cgcggatgcg gtgcactcgg cctgacggga cgggcacgcg ctcgagcagc
     1081 gccgtggccg ccatgtggta gttggagtcc gcgtggtccg gcgggaccgc gcgctcgtcg
     1141 ccccagaaga cctccacccg atcccacgcc acgcggtcgc gccaggccgg ctcggcgagc
     1201 agccggtaca tccgccgcgg cgtgctgccc ccggccagcg cgaccatgaa ccgtccccgc
     1261 gcgcccgcgg cggcggccgc cagcgtgacg aactcctcgg cccccgcctg ggccacggcg
     1321 tcggcgtcgt cgaagctgcg gaccgtgctc atgtcgtcac ggcctcgctc atccgcccgc
     1381 cgggggctcg acgtggccgc cgaacttctc gcgcatggcc gagagcacct tctcggcgaa
     1441 ggtgttctgt tgccgcgagc ggaaccggac gtagagcgag gccgtcagca cgtcggccgg
     1501 caccgcggtc tcgacggcct gctggaccgt ccaccgcccc tcgccggagt cttggaccac
     1561 gccggtgtac ttgccgagcg tcgggtgctc gacgagagcc tgggcggtga ggtcgagcag
     1621 ccacgagctg acgacgctgc cgcggcgcca cagctcggcg atgtccggga ggttgaggtc
     1681 gtagcgctga tccgcgggca tcgacgacga gtccgcgttc ttgagaatgt cgaacccctc
     1741 ggcgtacgcc tgcatgatgc cgtactcgat gccgttgtgg atcatcttca cgaagtgccc
     1801 ggcgcccacg gggccgcagt gcagccatcc gtcctcggcc gtcccgccgg agaccttctc
     1861 gcgcccgggc gtgcgcccga tgttgccgcg gccgggcgcc agcgtggtga agatgggggt
     1921 cagccgcttg atgacctcgg tctcgccgcc gaccatcagg cagtaaccgc gctcgaggcc
     1981 ccacacgccg ccgctggtgc ccgcgtcgac gtagtggatg ccgcggggca ggagcgcctt
     2041 ggcgcggcgg acgtcgtcct tgaacatcga gttgccgccg tcgacgatcg tgtcgccacg
     2101 ctccatccgc gccgcgaggt cgttcaccac ctgctcggtg accgcgccgg cgggcaccat
     2161 cacccacgcc acccgcgggg cacgcagctt cttgacgagg tcgtcgagcg aggtcgtgcc
     2221 cgcggcgccg tccttcgcga ggtcctgcac ggccttggcg tccgtcgcga agccgaccac
     2281 ctcatggccg ccgcgcatca gccgccgcgc catgttgccg cccatccgcc ccagcccgac
     2341 catcccgatc tgcatggttc ctcctcatcc tcgccctacc cgacgatggg aacagcaccc
     2401 gaaagacgac gcgcaacgca tcaggggtcg ccggacccgc tccaccccga ccccgccccg
     2461 gtcggcgctt tcgtccgcaa gcttcggacg cgggacacta cttttccccg cgggcgcgcg
     2521 cgacggtgcg cttggcggcg gcgaccaccg cgtcgggcgt gaaaccgaac ttggactgca
     2581 gctccttgag cggcgccgag gccccgaacg tcctcatgcc gatgatttcc ccctccgagc
     2641 caacccaccg cgcccacccg agggtcgagg cctgctcgac cgccacgcgc gcggtcatcc
     2701 cgggcgggag caccgcggcg cgatagtcct cgggctggcg atcgaagagc tcccacgacg
     2761 gcatgctcac cacacgcgcc ctcaccccct cggcggcgag cttctcccgc gcggccaggc
     2821 agagggcgac ctcgctgcca gtggccatga ggatcacgtc gggccggccg tcgggggcat
     2881 cggccagcac gtaggcgccc ttggcggtgc cgcgggcgtc gccgtgcttc gcgcggtcga
     2941 tcgtcggcag cgcctgccgc gacaggatca tggcgaccgg ctcgtggcga agcgtcatcg
     3001 tgtaccgcca cgcctccagc acctcgttgg cgtcacaggg gcggaacgtg atgagtcccg
     3061 ggatggcccg gagcgaggcg acctgctcga ccggctggtg cgtcggcccg tcctcgccca
     3121 cgccgatcga gtcgtgggtg aagatgtaca ccaccggcag ctccatcagg gcgctcagcc
     3181 ggatgggcgc ccgcatgtag tcgctgaaga tgaggaagcc ggagccgtag gcgcgcacct
     3241 tgctgagggc caggccgttg agcacggcgc ccatggcgtg ctcccgtacg ccgaagtgca
     3301 ggttgcgacc gtgcggcgtc tccgcggcga agtcgccagc gccctcgaac gtcagacgcg
     3361 tcttggtgga cggcgccagg tcggccgagc cgccgacgag ccacggcacg ttcttggcga
     3421 gcgtgttgag caccttggcc gaggcgtcgc ggccggcgac gcccttggga tcggccggga
     3481 acgggtcgag gcccttgtcc cagccctcgg gtagttgccg gcgctgcatg cggagaaggt
     3541 ggtcggccag ctcggggtac ttgtcccggt agcgcccgaa catctccacc cacgccatcc
     3601 gcgcggcctt gccacgcgcg ccgatgccgg cgccgaagtg ctcgcggacg ccgtcgggga
     3661 cgaggaactt cgcgtcctcg ggccagccgt aggcgcgctt ggtgagccgg atttcctcct
     3721 cgccgagcgg ctcgccgtgg gcggcgtgcg tgtcttgctt gtgcggcgaa ccccaggcga
     3781 tgtggctgtc aacgatgatc agggtcggcc ggtccggcgt gcgcttgaac gtcgccaagg
     3841 cgcgctcgag catcgagatg tcgttggcgt cgccgacgcg cagcacgttc cagccgtagc
     3901 ccaggaagcg cgtggccacg tcctcggtga aggccagagc ggtcgagccc tcgatggtga
     3961 tccggttgtt gtcgtagatc cagcacaggt tgcccaggcg caggtgaccg gccagcgagg
     4021 cggcctcccc ggagatgccc tccatgaagc atccgtcccc gcccagcgcg tacacgtcgt
     4081 agtcgaacat cgggaagtcg gggcggttga agcgcgcggc catccagcgc gccgcgatgg
     4141 ccatgcccac gctggtcgcg acgccggcgc cgagcgggcc cgtggtcgtc tcgacgcccg
     4201 aggtccaccg gtactccggg tggcctgcgc acttgctgtc gagctggcga aagcgcttga
     4261 tgtcgtcgag cgtgaccgag agcgagccga gccgctcgta ctggggattc acggccttca
     4321 cgccggccag gtgcagcatc gagtacagga gcatggaggc gtgccccatc gagagcacga
     4381 agcggtcgcg gttgccccag atcgggtcgt caggatcgaa gcgcagctgc cgctgccaga
     4441 gcgtatagat gacgggcgcc atggccatgg ccgtcccggg gtggccggag ttggcctgct
     4501 gcacggcgtc catcgagagc gtgcggatcg tgttgatgct gagctggtcg aggtcggcgc
     4561 tcatcgtggc tcctttcgct cgagcgcccg caccaccccg ccgtcccaga cgagcaccgt
     4621 gtcggcgacg tcgaggaaga ggccggtgtc gatgacgccg ggaatggcgc ggatcgcgcg
     4681 ctcgagcgcc cgggggtcgt cgatggggtc gagcgcgcaa tcgaggatga cgttccagtt
     4741 gtcgctgacg aaggggcgac cgtcggccat gcggagcgcg ggccggcagc cgagggccga
     4801 gagcttccgc tggcagaagg ccgtcgcgaa aggtagcacc tccaccggca cgcgcccgcg
     4861 gcggccgagt cgatcgacga gcttctcgct gtccacgagg atgacccggc gccgcgcggc
     4921 catggctacg atgcgctcgc gcacgagcgc gccgccccag cccttgacga gatcgaggtt
     4981 gggcgcgacc tcgtcggccc cgtccaccgc gatgtcgaga tcaccggcgt ccaggccgac
     5041 gagcgggatc cccaccgacc gcgccagcgc tgccgtcgcg tcggaggtcg gcacgcccgt
     5101 gatgcgcagc ccctggccga cgcgggcgcc cagcgacttg acgaaggcgg tggccgcgcg
     5161 gccggagccg aggccgacga tggtgtcctc gcgcacgaag ctcaacgccc ggtcggccag
     5221 cgcaccgagc aactgctcgc tcactatcgc catgtcggcc ctcgggctcg ccccttacag
     5281 gcgggggagc cagcggtgac gtcgtccctg ggggtcgcca gccggctcgt cacgagaatt
     5341 tcgtaactct aggcgcttcg cgcgatccag tcaaccagag ccgagcgatc accaatcaac
     5401 ctacctcggt cagcgcgtgc caggcgcgga gggtctcggc gacgctccag gcctgggcga
     5461 tgcagccgcg gggggtgtgc ggcggatcgc cgtcgaagat ctcggcgatc gagccgaggc
     5521 cgtactcgcc gaggtgatgg gccagcggcg ccagcaggtc gcgggcgccc gccgcatcgt
     5581 cgaacacgcg ccgccacgcg agcgcgaagg gaccgagcag ccagctccac accagcccct
     5641 ggtggtaggc gccatcgcgc tcgcgcacgc cgccctcgta gcggccgcgg taggcgggat
     5701 ggcccggtgc caggctccgg agccccatcg gggtgagcag atggcgcgcg cacacctcca
     5761 caacgcggcg ctgccgctcg ggcggcagcg ggctcgcggg cagcgagacg gcgaagacct
     5821 ggttcgggcg cagcgcgtcg tcgtcgccgc cggggccgtc gatgacgtcg tagcagcagc
     5881 cggtccgctc gcaccagaag cgcgcgaacg accgctcgac gcggtcggcg accgcggtcc
     5941 acgggtcgtc ccgccggccc aggcgccggg cgaaggcggc catgacgcgc agcgcgttgt
     6001 accagagagc gttgatctcg acgggcttgc cggcgcgcgg ggtgaccacc cagtcgccga
     6061 ccttggcgtc catccacgtg agctgcacgc cgggctcgcc ggcccgcagc aggccgtccg
     6121 ccgggtcctc accgatcccg tagcgcgtcc cgccgcggtg tgcgccgacg atggtctcga
     6181 gcgtcgggaa gagctcgccg atcaggacgt cgtctccggt cgcctcgtgg tacgcgcgga
     6241 cggcctcgac gtaccagagc gtcgcgtcca cggtgttgta ctcgggagcc tcgccggcgt
     6301 cggggaagcg gttcggcagc atgccacggt cgacgaagcg cgcgaacgtc gtcaggatgc
     6361 ggcgggcgat ctccggtcgg ccggtggtca gcgccagccc cgggagcgcg atcatcgtgt
     6421 cgcgccccca gtcgccgaac cagtgatagc ccgcgatgac ggtcatgccg tcagggtcgt
     6481 cggcgagcgg ccggcgggcg acgaactggt ccgcggcaag caccagctgg gcgatccagg
     6541 ccggcgctgc gggggcggtc ggctgagcgc ggcgccagcg gtcgagcagg tccgcctccc
     6601 aggcctcccg ccggtcccac gccgccgcgc cgtcgaccga gggcgccccc tccgccgaca
     6661 gcacgagggt gacctcccca cccggtggca gcgtcgcgcg gagcaccccc gcgcggaggt
     6721 ggtcctcgag gaactccaga ccgcggtcct gctcacgcgc caggtggaag ccgtagtacc
     6781 actcgtgctc cggcgcgatg accgcgccat cggcgaggag cagcagcggc cgcgcgccgt
     6841 cgaacgccgt cacgcggagc cccttgggca cgacctcgac gcgcatgggc cagcccccgc
     6901 gcgtcgcccc gtggtaatcg cggtggtcga cgagcaccgc cgcctcgagc gcgagcgggg
     6961 cctgcgcgcg tagcacgcgc cagcgcacgt acgtggtgtt ggcgccaggc tccatgaaga
     7021 tgcgcttctc cacgagcgcg tccgcgcacg cgtaggtcca gaccggcgtc gtcccgtcga
     7081 gcgcgaagcg caccaggtgg cggtggccgg ccggctccac gatgccggac gcccaggcgt
     7141 tggccccgag ggcgcgggtg acgccgccgt aggtcacggt ctcgtccacc ttcacggcca
     7201 tcagcgtgcg gccgagcggc ggtgacagcg ccgccacgag caggccgtga tagcgccgcg
     7261 tgagagcgcc ggcgatcgtg ccggaggcga agcctccgat gccgttggcg cagagccact
     7321 cgcggccagc ggccagctcg aagtcaccgg tcacctcgcg cccgacgtcg acgggagcga
     7381 gtggcacctg cccgggtcga ttcgcaggga gcctgtgcca gatgcgcgtg gcgccgccac
     7441 gggcgttctc cgcgttgccg acactgctcg ggcttggcgt catggcctca ccctccgtgg
     7501 gcgaattcgg gatagagcgt catgccgccg tccaccacga gcgtctggcc gtggacgtaa
     7561 tcggcggcgt ccgagaccag ccacgccacc acgcggccga cgtcctccgg cacgccgatg
     7621 cggccgtagg ggatcagccg gagcagcgcc gcacgcgcct cgggcgtctc ccacgccgac
     7681 cggttgatgg gcgtctggat cgctcccggc gcgacggcgt tgacccggat ccgccgcggc
     7741 gccagctcct gggcgagcga ctgcatgaac agcttcacgc cgcccttgga cgccgcgtag
     7801 ttggcatggc cggcccacgg aattatctcg tgcaccgacg acacgaagac gatcttcccg
     7861 gcggcccgcg agacgccggc tcggatgccc tggcggatca tggcgcgggt ggccgcccgc
     7921 gcggcgagga acgcgcccgt caggttcacg gcgatgacct cgttccactg cccgagcgtc
     7981 atctccgtca ccgcggcatc gcgctggatg ccggcgttgc tgacgacgat gtcgataccg
     8041 ccccaggcgg actggacgcg ctcgaacatc gcctcgacgt cggcctcgct ggccacgtcg
     8101 gcggccacgg ccatggcgtc ggacccggtg tccttgatct cggccacgac gcgcaaggcc
     8161 gcgtcccggt tgcgcgagta gttcacgacg acggcggccc cggcggtccc gagcgccatc
     8221 gcgatggccg cgccgatccc ggagccggcg ccggtcacca gggcccgctg gtccttcagc
     8281 gactgatcgt tcatcgcacg gcctcctacc cgctcgggcc cggcggtgag acaccggcgc
     8341 ggcgatgtcc ggttcggaca ccgtcactct acgccagggc taccagcgcc cgcgcgcgcg
     8401 ctccttcgcg ggcagggact cgaggcagca cgtccaccag cccgtccgga tcgtctcgac
     8461 gaactccggt ggcaggccct cgcgctccat ctcgcgcgcg agggcctcgt ggtcgtagtg
     8521 gaccggcacg aagtcgacct ccaggtcgtc tcccgccgtg agcagcgtgt accagacggc
     8581 cgggctgccg tcgttctcgg ggcgcccgag agcccccacg ttgacggcgt ggcggccgct
     8641 cggcagggcg cggtgccact tgagaccggt gtgcgtgacg cagaggacgt cggcatcgta
     8701 gcggtcgcag aagcgctcga gcaggccgac cggcgtcgcg ctctcccaca ggaactcgtt
     8761 cacctgccgc ggcgatccgt ggcacagcag gagccgccag gcgccgagcg tgagtcgcag
     8821 cgccggcgga agcgcgccga gccagcgccg gtgctcgctc gacgtccgcg cgagcgtgta
     8881 gcgatacgac accgccgcga agtcgttgtc gcgggggtcc gtgtagccgc agccgcagtc
     8941 ggcggcgccg gcggcgatcg acacgtcgta gttgccctgc acgcacgcca cggccgcgtc
     9001 gtggaggagc gggaagacac ggtccgggtg gggcccgaaa ccgccgagat cacccaggca
     9061 gtacacggcc tcggcgcccc gcccacgggc gtcggccagc gccgcctcca gggccaggta
     9121 gttgctgtag acgccgccca gcagggcgat ccggcgatgg gtcagtggtt gcggcacgcg
     9181 agtcccgtgg cgtggcaggt cacgcagacc ggatggtaga gcgacgccgg ccccagcgcg
     9241 tcggccagcc gtccgtcgct caggcgcgcg ccgggcaggc cggcgaggat cgggcaggcg
     9301 tagacgccgc cgtcggcgac gacgcgcgtc tcggcgcacg gcagacggct cacgtcgaag
     9361 gcttccaggt cgtccgcggt gaggcatcgc tcgtcggccc gccaggcgca gcgcccaagg
     9421 ggcaggacgg gcaggatctt cacgcgcggc cgccgcacgc cggccgcgag caggcacgcc
     9481 cgcagcctct cgtagagcgt ggggcccgcg cgcgacgcga cgatcttcgt cgccgtcacg
     9541 atcggcggca cgccccgcgc atcgagacgg cgcacggcgg cgagcgcccg cgcgaagctt
     9601 ccgcggccac gaacgcggtc gttggcctcc gcggtgtcgg cctcgacgct gacgcgcacc
     9661 tccagcgagt acgccgacga cgcggcgatg gcggccaggc gatccgcccc caggtcgtcc
     9721 acgagcgtcc cgttggtgag gacggtcgtc ggcgccaccg ccagcgccgt ctcgagaagg
     9781 gcgaggagat cggggtgcag gaacggctcg ccgccggtga agtacagctc cttgacgccc
     9841 agaccctccg cttcggcgat ggccgcccgg atcgccgtcg cgtccaggct ccccagccac
     9901 ggtgcccggg gaccgctggc attgaagcag tgacggcacg cgaggttgca gagggtgccc
     9961 gtcacctgca gccagacact gcgcaggtcg tgcagggcga cccacggcgt gggggctgcg
    10021 ggcgccggcg ccgcgcctcg ggtgcgcgga gtcatcgacc gacctcgacc ggaagccaca
    10081 tcgtgaaccg gctgccctcg cccgggcggc tctcgacggt gacgcggccg ccgtgggcct
    10141 cggccacgtg gcggacgagc gcgagaccga cgccgctgcc ccggcggccc tgcgtgtcgc
    10201 tgcgccccac ccggtagaac ttgtcgaaga tgcggccgtg ctcctcggca ggaatgccga
    10261 tgccctcgtc ggccaccgtg atggccagac agtcgccggc gacgcgggcc tcgatcgtga
    10321 gggacttctc gtcggccgag tacttgatgg cgttgtcgat gaggttcgcc agcgcctggc
    10381 ccacggcgtc ggcgtccatc ggcacctcgg gcacgtccgg gaccacgcgc acgtccacct
    10441 tgaagccgtg ctgctcgagc ggataggcga acgtttccag gacttcgcgc acgaggggct
    10501 ccacggcggt aggcacgcgc tcgtacgtgc gccgcccgcc ctcgatgcgg gagaagtcga
    10561 ggacgttgtc gatcaggcgc gtcagccgct cgctctcgcg cgcgatcacg cgatagtatt
    10621 cgtgccgcgc cgtctcgtcc ggcacgcggc cc
//
LOCUS       DATIAZ010000047         5701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_668868, whole genome shotgun sequence.
ACCESSION   DATIAZ010000047 DATIAZ010000000
VERSION     DATIAZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668868"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VLF19_01590"
     CDS             <1..315
                     /locus_tag="VLF19_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSE91970.1"
                     /translation="ARALGVRNAVIMFRHLLPNAMVATLTFMPFILNGSITTLTSLDF
                     LGFGLPPGSASLGEILAQGKANLQAPWIGLTGFVVLAVMLSLLIFIGEAVRDAFDPRK
                     VQ"
     gene            312..1961
                     /locus_tag="VLF19_01595"
     CDS             312..1961
                     /locus_tag="VLF19_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002969641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE91971.1"
                     /translation="MSAGNAGERADGAGGPLLSVRELSVSFAVEGGELHAVRGASFDV
                     ARGETVALVGESGSGKTVTALSIMQLLPYPKARHPSGRILFQGAELIDAPEPAMARVR
                     GDRIAMIFQEPMTSLNPLHTIEKQVNEVLLVHKGLPRTAARTRTLELLRLVGLPEAER
                     RLDAYPHQLSGGQRQRVMIAMALANEPDLLIADEPTTALDVTIQAQILLLLRGLQARF
                     GMALLLITHDLGVVKKMAERVCVMREGEVVEAGPVAEVFERPRHPYTKRLLAAEPRPM
                     PRAPRVDAPVVVEAKDLRVWFPVRRGLLRRVVDHVKAVDGVSLTVREGQTLGVVGESG
                     SGKTTLGLALLRLQACRGRIRFAGRDLHALTARAVRPLRREMQVVFQDPYGALSPRMS
                     IAQIVGEGLGVHGVGATAGERRHLIDEALREVGLDPALRDRYPHEFSGGQRQRIAVAR
                     AMVLKPRFVVLDEPTSALDMSVQAQIVDLLRELQARHRLAYLFISHDLKVVRAMSDEI
                     LVMRAGRVVEEGPAERIFEQPEQAYTRALMAAALDLEAVAS"
     gene            2007..2984
                     /locus_tag="VLF19_01600"
     CDS             2007..2984
                     /locus_tag="VLF19_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE91972.1"
                     /translation="MDIRHRFVETNGVRMHVAEAGQGPLVVLCHGFPESWYSWRHQLV
                     ALARAGFHAVAPDQRGYGQTDQPEAIDQYTLFHLVGDVVGLLDALGAPTAVIAGHDWG
                     APVAWSAALLRPDRFRGVIGLSVPYLPRGSVRPTTVMPRTGDTIFYQLYFQEPGVAEA
                     ELERDPRDTIRRVLYWGSGDAPRPPRTVGSGDVGMVRRDVGFLGGRQAPAALPPWVTE
                     ADVEFYAGEFARAGFRGGLNWYRNIDRNWELMAPWTGARLTAPALYVAGDRDLVLGFR
                     GMDRLLASLRGFVPNLTETTILPGCGHWTQQERASEVNAAILKFLKGLS"
     gene            2981..4453
                     /locus_tag="VLF19_01605"
     CDS             2981..4453
                     /locus_tag="VLF19_01605"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HSE91973.1"
                     /translation="MKTHIAGGWVVADNGEGHELHEQGSVVFEDDRIVHAGGPYTGPA
                     DTRIEARGRLVSPGFINTHVHTTGGGGDYLLLDMTRNDYRTANYMGFAAPLRGTMTPP
                     AVAATGALRAFTFLHTLKNGTTTHIDVGGLRGDWEGYARLVDHLGVRVYGSPPFRDRD
                     TYMDARGRLTYEEDAGVGRERLREAVDFVRKFDGAAQGRLRGMLNAAQVETCTEGLLR
                     AGKDAARELGVPIHTHAGGNLIEFQRIMDEHRTTPIRFLADIGFLDDRALIGHGVFTT
                     AHPWTHYPFGDDVRVLAERGATVGHCPYKYAKMAITLHSFQRCVDAGVTVALGTDTYP
                     MDIVSELRWASILAKVTDGSYQAGQARDVFNAATLGGCRFLRRDDLGRLAPGAKADIL
                     IIDLEHLGAGVYADPIKALVDAGTGRDVRTVIVDGQVLVQDGRLTRVDEAEVYAKARE
                     ATRRYWGNVPSWRWDGAGVERIIPPAFPLHRAGNDTPAQS"
     gene            complement(4468..5268)
                     /locus_tag="VLF19_01610"
     CDS             complement(4468..5268)
                     /locus_tag="VLF19_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HSE91974.1"
                     /translation="MSETALETLLRRDRVVVGAALAALIAGAWLYLLYLASAMSAMAM
                     PDMPGRAMAMPAMHAWSWVEVGALVVMWTVMMVAMMMPSATPMILVFAAIHRRRAIEG
                     RPTVPTAIFLLGYLVMWTIYSVAAALAQAGLHAAALLSPAMAATSPLLAGGLLVVAGV
                     FQWTPLKRTCLAGCRSPLSFFMTGWREGRRGAFRMGLHHGLYCLGCCWALMALLFVAG
                     VMNLLWVAAIAVTVLVEKVVPRGDLVGRLAGVALVTAGVLLVARVVAG"
     gene            complement(5286..>5701)
                     /locus_tag="VLF19_01615"
     CDS             complement(5286..>5701)
                     /locus_tag="VLF19_01615"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HSE91975.1"
                     /translation="AIHEGNGKAAFIFDVAATDEQVEALSQILAGKLGGLPWELLGST
                     FKVVGILKRKITVEGSGRKSTFRIEGVGEGRGDTLKNPVTGEDHIVNVDLPTGFIWKK
                     GEAGQGSFHASVEDLSVQFDKTNWIYYQYDWSNTT"
BASE COUNT          820 a         2065 c         2030 g          786 t
ORIGIN      
        1 gcccgcgcgc tcggcgtccg gaacgccgtc atcatgttcc gccacctgct gccgaacgcc
       61 atggtcgcca cgctcacctt catgcccttc atcctgaacg gctcgatcac cacgctgacc
      121 tcgctcgact ttctcggctt cgggctgccg ccgggctcgg cctcgctcgg cgagatcctc
      181 gcgcagggca aggccaacct ccaggcgccg tggatcgggc tcaccggctt cgtcgtgctc
      241 gcggtgatgc tctcgctcct catcttcatc ggcgaggccg tgcgggacgc cttcgatccc
      301 cgcaaggtcc aatgagcgcc ggcaacgccg gggagcgggc ggacggcgcg gggggccccc
      361 tcctctccgt gcgcgagctc tcggtgtcgt tcgccgtgga gggcggcgag ctgcacgcgg
      421 tccgcggcgc gtcgttcgac gtcgcgcgcg gcgagacggt ggcgctggtg ggcgagagcg
      481 gctcgggcaa gacggtcacc gcgctctcga tcatgcagct cctcccctac cccaaggccc
      541 ggcacccgtc cggccgcatc ctgttccagg gcgcggagct gatcgacgcg cccgagccgg
      601 cgatggcgcg cgtgcgcggc gaccgcatcg cgatgatctt ccaggagccg atgacgtcgc
      661 tgaacccgct gcacaccatc gagaagcagg tgaacgaggt cctcctcgtc cacaagggac
      721 tcccgcggac ggccgcgcgg acgcgcacgc tggagctgct ccgcctcgtc ggcctgcccg
      781 aggccgagcg ccggctcgac gcctacccgc accagctctc cggcggtcag cgccagcggg
      841 tgatgatcgc gatggccctc gccaacgagc ccgacctgct catcgccgac gagcccacga
      901 ctgccctgga cgtcaccatt caggcgcaga tcctcctgct gctgcgcggg ctgcaggcca
      961 ggttcggcat ggcgctgctg ctcatcaccc acgatctcgg cgtcgtcaag aagatggccg
     1021 agcgcgtgtg cgtgatgagg gagggcgagg tcgtcgaggc gggacccgtg gccgaagtct
     1081 tcgagcgccc gcgccacccg tacacgaagc ggctgctcgc cgccgagccg cgcccgatgc
     1141 cgcgcgcgcc gcgtgtcgac gcgcccgtgg tcgtcgaggc caaggacctc cgtgtgtggt
     1201 tcccggtgcg gcgcggactg ctgcgccgcg tggtggacca cgtcaaggcc gtggacggcg
     1261 tgagcctcac ggtccgcgag ggccagacgc tcggcgtggt gggcgagagc ggctcgggca
     1321 agaccacgct cgggctcgcg ctgctgaggc tccaggcctg ccgggggcgc atccggttcg
     1381 ccggccgcga cctccacgcg ctgacagcgc gtgccgtccg gccgctgcgg cgcgagatgc
     1441 aggtggtctt ccaggacccg tacggtgcgc tgtcgccgcg gatgtccatc gcgcagatcg
     1501 tcggcgaggg gctcggcgtg cacggcgtgg gggcgacggc gggcgagcgc cggcacctca
     1561 tcgacgaggc gctccgcgag gtcggcctcg atcccgcgct ccgcgatcgc tacccgcacg
     1621 agttctcggg cggccagcgt cagcgcatcg ccgtcgcgcg ggcgatggtg ctgaagccgc
     1681 gcttcgtggt cctcgacgag ccgacgtccg cgctcgacat gtcggtccag gcgcagatcg
     1741 tcgatctcct gcgcgagctg caggcgcgcc accggctggc gtacctcttc atcagccacg
     1801 acctcaaggt cgtccgcgcg atgagcgacg agatcctcgt catgcgcgcc gggcgcgtcg
     1861 tcgaggaggg gccggccgag cgcatcttcg agcagccgga gcaggcgtac acgcgcgcgc
     1921 tgatggccgc cgcgctcgac ctggaggccg tcgcgtcatg atctcgtcga cacgggcacc
     1981 agagggaagg cggtagaatc ccggccatgg acatccggca ccgcttcgtc gagaccaacg
     2041 gcgtccgcat gcacgtcgcc gaggccggcc aggggccgct cgtggtgctg tgccatggtt
     2101 ttcccgagtc gtggtactcg tggcgtcatc agctcgtggc cctggcgcgg gccggcttcc
     2161 acgccgtggc gcccgatcag cgcggctacg ggcagaccga ccagcccgag gcgatcgatc
     2221 agtacaccct cttccacctg gtcggcgacg tcgtcggcct cctcgacgcc ctcggcgcgc
     2281 cgacggcggt gatcgccggc cacgactggg gcgcgccggt ggcgtggagc gcggcgctgc
     2341 tgcggcccga ccggttccgt ggcgtgatcg ggctgagcgt gccgtacctg ccgcgcggct
     2401 cggtgcgccc cacgaccgtc atgccgcgca ccggggatac gatcttctac cagctctact
     2461 tccaggagcc cggggtcgcg gaggccgagc tcgagcgcga cccgcgcgac acgatccgcc
     2521 gcgtgctgta ctggggatcg ggcgacgcgc cccgcccgcc ccgcacggtg gggtcgggcg
     2581 acgtcgggat ggtgcggcgc gacgtcggct tcctgggggg ccggcaggca ccggcggccc
     2641 tgccgccgtg ggtcaccgag gccgacgtcg agttctacgc cggcgagttc gcgcgcgcgg
     2701 gcttccgcgg cggcctcaac tggtaccgca acatcgaccg caactgggag ctgatggcgc
     2761 cgtggacggg cgcgaggctg acggcgccgg cgctctacgt ggccggcgac cgcgacctgg
     2821 tgctgggctt cagggggatg gaccgcctgc tggcgtcgct ccgggggttc gtgcccaacc
     2881 tcacggagac gaccatcctg ccgggctgcg ggcactggac gcagcaggag cgggcgagcg
     2941 aggtgaacgc ggcgatcctg aagttcctga aggggctctc atgaagactc acatcgcggg
     3001 cggctgggtc gtcgccgaca acggcgaagg ccacgaactg cacgagcagg gcagcgtggt
     3061 cttcgaggac gaccgcatcg tccacgccgg cgggccgtac acgggccccg ccgacacgcg
     3121 catcgaggcc cgcggacggc tcgtctcgcc ggggttcatc aacacgcacg tgcacacgac
     3181 gggcggcggc ggcgactacc tgctcctgga catgacgcgc aacgactacc gcaccgcgaa
     3241 ctacatgggc ttcgcggcac cgctccgggg cacgatgacg ccgccagccg tggcggccac
     3301 gggcgcgctg cgcgccttca ccttcctgca cacgctgaag aacggcacca cgacgcacat
     3361 cgacgtgggg ggcctgcgcg gcgactggga gggctacgcg cgcctggtgg accacctcgg
     3421 cgtccgcgtc tacggcagcc ccccgttccg ggaccgcgac acctacatgg acgcgcgcgg
     3481 gcggctcacc tacgaggagg acgccggcgt cggccgggag cgcctgcgcg aggcggtcga
     3541 cttcgtcagg aagttcgacg gcgcggcgca ggggcggctg cgcggcatgc tgaacgcggc
     3601 gcaggtcgag acctgcaccg aggggctgct acgcgctggc aaggacgccg cccgggagct
     3661 cggcgtgccc atccacaccc acgccggcgg caacctcatc gagttccagc ggatcatgga
     3721 cgagcaccgg acgacgccga tccgcttcct ggccgacatc ggcttcctcg acgaccgcgc
     3781 gctcatcggc cacggcgtgt tcacgaccgc gcatccgtgg acgcactacc cgttcggcga
     3841 cgacgtgcgg gtgctcgccg agcgtggcgc taccgtcggc cactgcccgt acaagtacgc
     3901 caagatggcg atcacgctgc actcgttcca gcgctgcgtg gacgccggcg tcaccgtggc
     3961 cctcggcacc gacacctacc cgatggacat cgtgtcggag ctgcgctggg cgtcgatcct
     4021 cgcgaaggtc accgacggca gctaccaggc ggggcaggcg cgcgacgtct tcaacgcggc
     4081 cacgctcggc ggctgccggt tccttcgccg cgacgacctg ggccgcctgg ccccgggcgc
     4141 caaggccgac atcctgatca tcgacctcga gcacctcggc gccggcgtct acgccgatcc
     4201 catcaaggcg ctcgtggacg ccggcaccgg gcgcgacgtg cgcacggtga tcgtggacgg
     4261 ccaggtgctc gtgcaggacg gccgcctcac gcgcgtggac gaggcggagg tgtatgcgaa
     4321 agcgcgcgag gccacccggc gctactgggg caacgtgccg agctggcgct gggacggcgc
     4381 cggggtcgag cgcatcatcc ccccggcctt ccccctgcac cgcgccggca acgacacgcc
     4441 ggcgcagtcc tgacgtccgg cgcgcggtca gcccgcgacg acgcgggcca cgagcagcac
     4501 gccggcggtg acgagcgcca cgccggcgag acggcccacc aggtcgccgc ggggcacgac
     4561 cttctccacc aggacggtca ccgcgatggc ggcgacccag agcaggttca tgacgcccgc
     4621 cacgaacagc agcgccatga gtgcccagca gcacccgagg cagtagaggc cgtggtgcag
     4681 gcccatccgg aacgcgccgc ggcggccctc gcgccagccg gtcatgaaga aggacagcgg
     4741 tgagcggcag ccggccaggc acgttcgctt gaggggcgtc cactggaaca cgccggcgac
     4801 gacgaggagg cctccggcga gcagggggct cgtggccgcc atcgccggcg acagcagcgc
     4861 ggcggcgtgg agcccggctt gcgccagcgc cgcggcaacg ctgtagatcg tccacatcac
     4921 gaggtagccg aggaggaaga tcgccgtcgg caccgtgggg cgaccttcga tcgcgcggcg
     4981 ccggtggatg gctgcgaaca cgaggatcat cggcgtggcc gacggcatca tcatggcgac
     5041 catcatcacg gtccacatga cgacgagtgc gccgacctcc acccagctcc aggcgtgcat
     5101 cgcgggcatg gccatggccc tccccggcat gtcgggcatc gccatggccg acatcgcaga
     5161 ggccaggtag aggagataga gccaggcgcc ggcgatgagc gccgccagcg ccgcgccgac
     5221 cacgacacgg tcgcgacgga gcagggtttc gagcgccgtc tcgctcatcg cgcgggtccg
     5281 ccgcttcagg tggtgttcga ccagtcgtac tggtagtaga tccagttcgt cttgtcgaac
     5341 tggacggaca gatcctcgac cgacgcgtgg aacgagccct ggccagcctc gcccttcttc
     5401 cagatgaagc ccgtgggcag gtcgacgttg acgatgtggt cctcgcccgt gaccgggttc
     5461 ttcaaggtat caccacgccc ctcgcccacg ccctcgatgc ggaacgtgct cttgcgcccg
     5521 ctgccctcga cggtgatctt cctcttcagg ataccgacga ccttgaaggt cgatccgagc
     5581 agctcccacg gcaacccgcc gagcttgccg gcgaggatct gggagagcgc ctcgacctgc
     5641 tcgtcggtgg cggccacgtc gaagatgaac gcggccttgc cgttgccctc gtggatcgcc
     5701 t
//
LOCUS       DATIAZ010000048         5893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1194841, whole genome shotgun sequence.
ACCESSION   DATIAZ010000048 DATIAZ010000000
VERSION     DATIAZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194841"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VLF19_01620"
     CDS             <1..1793
                     /locus_tag="VLF19_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSE91976.1"
                     /translation="VLPVRAEARGRVPGIVHDRSQSGQTIFVEPADLVEANNDLVQLS
                     RDEEAEAARVLAELTDAVRERLGEMDGLVDVVTELDWTFARAALAERMRATAPTVTER
                     GPVAIAGARHPLLLAQEWQDPARPVVPVDLELGPDRPAVIITGPNAGGKTIVLKTLAL
                     HALMAQTGCHLPAEEGARLPAFTRVHAIIGDDQSVAENLSTFSAFVKQVREILAEADD
                     RSLVLLDELGAGTDPDEGAALAQAILEELAGRGALVLATTHLEPLKAFASTHPRARNA
                     SVEFDTTTLAPTFRLRYDRPGQSLALTIAARLGLSPALIERAQSHRSMHAARLGELLQ
                     RLDEYTRGEAERTMAIERREQETASRLAAAREAETTAQDRARELVARAKAEAAQLVAD
                     VRRAVSAEWERLKRGDRSRRDVEESRRRINAAAARTVAAPAPPTPEAAAALVPGARVA
                     AEHLGLRGELLALEGGTATVRAGNVTVRVPVQALRLAAGLDPPAPAGVAGRARAAISV
                     PEKTGVGSELHLLGRTTDEARDLVEQYLDDAFMAGLPTVRLVHGKGTGALRKAIRDLL
                     AAHPLVESYRDGEPAEGGAGATVAALKVGA"
     gene            1806..3662
                     /gene="dnaG"
                     /locus_tag="VLF19_01625"
     CDS             1806..3662
                     /gene="dnaG"
                     /locus_tag="VLF19_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSE91977.1"
                     /translation="MAGYSPALLDEVRTGIDLVDLIGRFVNLRKTGANFKGLCPFHSE
                     KTPSFMVNPKKGIFHCFGCGAGGDAFGFLMRQDRLTFPEAVRTLARQAGVSLPDDRGA
                     VPDDSGREELYRVMDVAAKFYAEALWKPGGERAREYLDRRGIEPEAARRFALGLAPDA
                     WDALLGFMRSQRVAPEALEAAGLVIRRESGTGVYDRFRGRLMFAIRDLQGRVVAFGGR
                     GFGDEQPKYLNSPETPIYSKGSLLYAIDTARTTIREKNRALLVEGYVDCLMAHQHGFT
                     ETVAALGTAFTPAQLGLLRRYCEAVVTFFDADAAGQKAAERAAELLEPSGAGAAWAVN
                     RTGGFEAGPAFRLKVALLPAGHDPDTFLRAHGAEAFTARIAAARSLLGYALDRAIADP
                     DGASGPRARANAFARVALMLAKVADAEEAATLSREAAVKLGVDPTQLWIESQRLQTAL
                     RRPPASTAPAAVPAPGRAEDRALVRLLLRHGEARESLLPLVEERELAAEPLRAVLVAL
                     RARPDATPESLLTDLDDAGRGALAALVVDEDEAGTTDPQASIADFRRRLERAQRLRRI
                     RAVSRSIAEAQATDAPLEDRLRMLERESREVHATARGAHHPGPDGSPRSPDA"
     gene            3659..5497
                     /gene="rpoD"
                     /locus_tag="VLF19_01630"
     CDS             3659..5497
                     /gene="rpoD"
                     /locus_tag="VLF19_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSE91978.1"
                     /translation="MSDEPKLEELDRLISVGKQKGYLTYDEVNDALPSDIVSLDQLDD
                     IMMMFGAMDIEVVDSAKAARLPSEIQRAVPAEEPDDEGGDAIDLTPGPVGRTEDPVRL
                     YLREMGRVALLTREGEIALAKRIEEGKNQVANAILSTNLALERFRELREQLRQDDISI
                     KEVVDVNEEEFTEEKEIELKRQVINAFGQVDRLLRDRDALVEQARKLKAKSGGRKKVK
                     GGKEPTWRKLELKGQQHQDRVLKKLRELNIGNQIIDREDGDTVRRGLVQRLRDLVEDI
                     ERAERVIALWTNGRRPSAEEVQNLIYVSFRDPSANGANGTFADLHLRAAKKFTSPKQQ
                     QIWVAQQEIKAAEGRANARADEIKRVMAVIKQGQAKAAQAKKEMVEANLRLVISIAKK
                     YTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIR
                     IPVHMIETINKLIRTSRQLVQELGREPTPEEIAQKMDVPVDKVRKVLKIAQEPISLET
                     PIGEEEDSHLGDFIEDKQVVSPVESIIGLSLREQTNKVLNTLTPREEKVLRLRFGLSD
                     GCEHTLEEVGQDFAVTRERIRQIEAKALRKLRHPSRSKKLRSFLES"
     gene            5525..5596
                     /locus_tag="VLF19_01635"
     tRNA            5525..5596
                     /locus_tag="VLF19_01635"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5557..5559,aa:Ile,seq:cat)
BASE COUNT          941 a         2113 c         2092 g          747 t
ORIGIN      
        1 acgtcctgcc ggtgcgcgcc gaggcccgcg gacgcgtccc gggcatcgtt cacgaccgct
       61 cgcagagcgg ccagacgatc ttcgtcgagc ccgccgatct cgtggaggcc aacaacgacc
      121 tggtccagct atcgcgcgac gaggaggccg aggccgcccg cgtcctggcc gagctgaccg
      181 acgccgtgcg ggagcggctg ggcgagatgg acgggctcgt cgacgtcgtg accgaactcg
      241 actggacgtt cgcccgcgcg gcgctcgccg agcgcatgag ggcgacggcg cccaccgtga
      301 ccgagcgcgg acccgtcgcg atcgccgggg cccgccaccc cctccttctg gcgcaagagt
      361 ggcaggaccc ggcacgcccc gtcgtgcccg tggacctcga gctgggaccg gaccggcccg
      421 ccgtgatcat caccgggccg aacgcgggcg gcaagacgat cgtgctcaag acgctcgcgc
      481 tgcacgcgct gatggcccag acgggctgtc acctgccggc ggaggagggc gcgcgactcc
      541 cggcgttcac ccgcgtgcac gccatcatcg gcgacgacca gagcgtggcc gagaacctct
      601 cgaccttctc cgcgttcgtc aagcaggtcc gcgagatcct ggccgaggcc gacgaccgct
      661 cgctggtgct gctcgacgag ctgggtgccg gcacggatcc cgacgagggc gcggcgcttg
      721 cccaggcgat cctcgaggag ctggccggac gcggtgcgct cgtgctggcg accacgcacc
      781 tggagccgct caaggcgttc gcgagcacgc accctcgcgc gcgcaacgcc tccgtcgagt
      841 tcgacacgac caccctggcc ccgacgttcc gcctccgcta cgatcgcccc ggccagagcc
      901 ttgcgctcac gatcgccgca cgcctgggtc tctcccccgc gctgatcgag cgcgcgcagt
      961 cgcaccgctc gatgcatgcg gcgcggctcg gcgagctgct acagcggctc gacgagtaca
     1021 cgcgcggcga ggccgagcgc acgatggcca tcgagcgccg cgagcaggag acggcgtcgc
     1081 ggctggccgc cgcccgcgag gccgagacga ccgcgcagga ccgtgcgcgc gagctcgtcg
     1141 cccgggccaa ggccgaggcc gcccagctcg tggccgacgt gcgccgtgcg gtgagcgcgg
     1201 aatgggagcg tctcaagcgt ggggaccgct cccgccgcga cgtcgaggag agccgacggc
     1261 ggatcaacgc ggccgccgcc cggaccgtgg ccgcccccgc tccaccgacg ccggaggccg
     1321 ccgcagcgct cgtgccgggg gcgagggtcg ccgccgagca tctcgggctg cggggggagc
     1381 tgctggcgct cgagggtggc acggccacgg tgcgagccgg caacgtcacc gtgcgggtcc
     1441 ccgtgcaggc gcttcggctg gccgccgggc tcgacccgcc ggcgcccgcc ggcgtcgccg
     1501 gccgagcgcg ggcggccatc agcgtaccgg agaagacggg ggtggggtcc gagctgcacc
     1561 tgctgggccg caccaccgac gaggctcgcg acctggtcga gcagtacctt gacgacgcct
     1621 tcatggccgg cctgcccacc gtgcgcctcg tccacggcaa gggcaccggc gccttgcgca
     1681 aggccatccg ggacctgctg gcggcgcacc cactggtcga gtcttaccgc gacggggagc
     1741 ctgccgaggg aggcgccgga gcaacggtgg cggcgctcaa ggtcggagcg taggatggag
     1801 ggctgatggc cggttactcg cccgcgctcc tggacgaggt ccggacgggc atcgacctcg
     1861 tcgacctcat cgggcggttc gtcaatctcc gcaagaccgg agcgaacttc aagggtctct
     1921 gccccttcca cagcgagaag accccgtcgt tcatggtcaa tcccaagaag ggcatcttcc
     1981 actgcttcgg ctgcggcgcc ggcggggacg ccttcggctt cctcatgcgc caggaccggc
     2041 tgaccttccc ggaggccgtg cgcacgctcg cccggcaagc cggcgtgagc ctgcccgatg
     2101 atcgcggcgc cgtccccgac gactcggggc gcgaggagtt gtaccgcgtg atggacgtcg
     2161 ccgcgaagtt ctacgccgag gcgctgtgga agccgggcgg ggagcgcgcc cgggaatacc
     2221 tcgatcggcg cggcatcgag ccggaggccg cgcggcgctt cgcgctcggg ctcgcgcccg
     2281 acgcctggga cgccctgctc gggttcatgc gaagccagcg agtggcgccg gaggctctgg
     2341 aagcggccgg gttggtgatc cggcgcgaga gcggcaccgg cgtctacgac cgcttccgcg
     2401 gccgactcat gttcgccatc cgcgatcttc agggccgcgt ggtcgccttc ggcggacgcg
     2461 gtttcggcga cgagcagccg aagtacctca actctcccga gacgccgatc tacagcaagg
     2521 gcagtctcct ctacgcgatc gacacggccc gcaccaccat ccgcgagaag aaccgcgcgc
     2581 tgctggtgga aggctacgtg gactgtctga tggcccacca gcatggcttc acggagacgg
     2641 tcgccgcgct cggcacggcc ttcacgcccg cgcagctcgg tctcctccgg cgctactgcg
     2701 aggcggtcgt gaccttcttc gacgccgacg ccgcaggcca gaaggccgcc gagcgcgcgg
     2761 cggagctgct cgagccgagc ggtgccggcg cggcgtgggc cgtgaaccgc accggcgggt
     2821 tcgaggccgg ccccgccttc cggctgaagg tcgcgctgct gccggcgggc cacgatcccg
     2881 acacgttcct gcgcgcacac ggggccgagg cgttcaccgc ccgcatcgcg gccgcgcgca
     2941 gtctcctcgg gtacgccctc gaccgggcca tcgcggaccc ggacggcgcc tccggacccc
     3001 gggcgcgcgc caacgccttc gcacgggtgg cgctgatgct cgcgaaggtc gccgacgccg
     3061 aggaggccgc gacgctgtct cgcgaggcgg ccgtcaagct cggggtggac ccgacccagc
     3121 tctggatcga gtcgcagcgg ctgcagacgg cgctccggcg accgccggcg tccacggcgc
     3181 cggccgcggt gccggcgccg ggtcgtgccg aggaccgcgc gctcgtccgc ctgctcctgc
     3241 gccacggcga ggcgcgcgag tccctgctgc cgctggtgga agagcgcgag ctcgccgccg
     3301 agccgctgcg ggccgtgctc gtcgcgctcc gcgcgcgtcc cgacgctacc cccgagagtc
     3361 tgttgaccga cctcgacgac gccggccgcg gcgcgctcgc tgcgctcgtg gtggacgagg
     3421 acgaggcggg gacgaccgat ccccaggcca gcatcgccga cttccggcgg cgcctcgagc
     3481 gggcccagcg cctgcggcgg atccgcgccg tcagccgcag catcgccgag gcgcaggcca
     3541 cggacgcacc gctcgaggac cgcctccgca tgctcgagcg ggagagccgc gaggtccacg
     3601 ccacggcgcg gggtgcccac cacccgggac ccgacgggtc cccaaggagt ccagacgcat
     3661 gagtgacgag ccgaagctgg aggagctgga tcgcctgatc tcggtgggca agcaaaaggg
     3721 ctacctcacc tacgacgagg tgaacgacgc cctcccgtcg gacatcgtgt cgctcgacca
     3781 gctcgacgac atcatgatga tgttcggcgc catggacatc gaggtggtgg attcggcgaa
     3841 ggccgcgcgc ctgccgtcgg agatccagcg cgcggtaccg gccgaggagc ccgacgacga
     3901 gggcggcgac gcgatcgacc tcacgcctgg ccccgtgggc cgcaccgagg acccggtgcg
     3961 gctctacctc cgcgagatgg ggcgggtggc gctgctcacg cgcgagggcg agatcgcgct
     4021 cgccaagcgg atcgaggaag gcaagaacca ggtcgccaac gccatcctca gcaccaacct
     4081 cgccctcgag cggttccgcg agttgcgcga gcagctccgc caggacgaca tctccatcaa
     4141 ggaggtcgtg gacgtcaacg aggaggagtt caccgaggag aaggagatcg agctcaagcg
     4201 ccaggtcatc aacgccttcg gccaggtcga ccgcctcctc cgggaccgcg acgcgctcgt
     4261 cgagcaggcg cgcaagctca aggccaagtc gggcggccgc aagaaggtca aggggggcaa
     4321 ggagccgacc tggaggaagc tcgagctgaa gggccagcag caccaggacc gggtcctcaa
     4381 gaagctgcgc gagctgaaca tcggcaacca gatcatcgac cgcgaagacg gcgacaccgt
     4441 ccgccgtggc ctcgtgcagc ggctgcgcga cctggtcgag gacatcgagc gcgccgagcg
     4501 ggtgatcgcg ctctggacca acgggcgccg gccgtccgcg gaggaggtgc agaacctcat
     4561 ctacgtctcc ttccgcgatc cgtccgccaa cggtgccaac ggcaccttcg ccgacctgca
     4621 cctcagggcc gccaagaagt tcaccagccc caagcagcag cagatctggg tcgcccagca
     4681 ggagatcaag gccgcggagg gacgcgccaa cgcccgggcc gacgagatca agcgcgtcat
     4741 ggcggtgatc aagcagggcc aggccaaggc cgcccaggcc aagaaggaga tggtcgaggc
     4801 caacctccgc ctcgtgatct ccatcgccaa gaagtacacg aaccgcggcc tgcagttcct
     4861 cgacctcatc caggagggca acatcggcct catgaaggcc gtggacaagt tcgagtaccg
     4921 tcgcggctac aagttctcga cctacgcgac gtggtggatc cgccaggcga tcacgcgcgc
     4981 catcgccgac caggcccgca ccatccgcat ccccgtgcac atgatcgaga cgatcaacaa
     5041 gctcatccgg acctcgcgtc aactggtgca ggagctgggc cgcgagccca ccccggagga
     5101 gatcgcgcag aagatggacg tgccggtgga caaggtccgc aaggtgctca agatcgcgca
     5161 ggagcccatc tcgctggaga cgcccatcgg tgaggaagag gacagccacc tgggtgactt
     5221 catcgaggac aagcaggtgg tctcgccggt ggaatcgatc atcgggctca gcctccgcga
     5281 gcagacgaac aaggtcctca acacgctgac gccgcgcgag gagaaggtgc tgcgcctccg
     5341 cttcgggctc tccgatggct gcgagcacac gctggaggag gtcggccagg acttcgcggt
     5401 gacccgtgag cgcatccgtc agatcgaggc caaggcgctc cgcaagctgc gtcatccctc
     5461 gcgctccaag aagctccgca gcttcctgga gtcgtgatgt acgctgaggc gttcatgagc
     5521 ccgcgggccc atagctcaac ggcagagcag ccggttcata cccggtcggt tcctggttcg
     5581 agtccaggtg ggcccactat tgtagcgacg ggttggagcc cggttcgaac cgagggtggc
     5641 ctgttcgatg agcgcgtggc accgcaggcc ctggcaaccg tcccggaggg ggcggcaccg
     5701 cacgcccggt cgtcgcgcga gcggcgtcgt gactgagctg cagacgctcg tgcatctcca
     5761 ggaattcgac acgcggatat ctgctctcga ggtcgaggcg gcgcgtctcc ccaagcagat
     5821 cgaggcgctc cacgccgcgc tggccgaggc caagaagacg atggagacgg tcaagggccg
     5881 gctcgacgcc acg
//
LOCUS       DATIAZ010000049         5333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1195125, whole genome shotgun sequence.
ACCESSION   DATIAZ010000049 DATIAZ010000000
VERSION     DATIAZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195125"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VLF19_01640"
     CDS             <1..180
                     /locus_tag="VLF19_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSE91979.1"
                     /translation="AYSVSGEYAMLKAAAQLGWLDEDRAMMEALTGIRRAGADIVITY
                     FAKDVARRLERKREP"
     gene            198..380
                     /locus_tag="VLF19_01645"
     CDS             198..380
                     /locus_tag="VLF19_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE91980.1"
                     /translation="MGAGATFEATRERGARAERDLVEPWDEIAGAVSHVVHRLTLGEL
                     SARVRERLSLERATYV"
     gene            383..1303
                     /gene="cysK"
                     /locus_tag="VLF19_01650"
     CDS             383..1303
                     /gene="cysK"
                     /locus_tag="VLF19_01650"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HSE91981.1"
                     /translation="MDRPLVAESVLDLIGWTPMVRLNRLPSPKGARVLAKLESVNPGG
                     SVKDRIGIAMLEDAERRGALRPGGTIVEPTSGNTGIGLAMAAAVKGYRLILTMPDDMS
                     VERQRLLARFGAEIHLTPAIEGMTGAVFAAQELLREHPDYFMPQQFENPANPGVHRRT
                     TALEILSAVGDRRIDAFVAGVGTGGTVTGVGEVLKEKMPGVRVVAVEPARSAVLSGGR
                     ARPHAIQGIGASFVPGVLNRAVLDSIVQVRDEDAIAWSRRLAREEGLLVGISAGAAVF
                     AACGVAGDLSPEQLVVTVLPDTGERYLSMG"
     gene            1445..1726
                     /locus_tag="VLF19_01655"
     CDS             1445..1726
                     /locus_tag="VLF19_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSE91982.1"
                     /translation="MAVMVYIPTPFRRATGNRDRVEVAAGDVGDLLDGLERQFAGLRG
                     LVRNDSGEVHHHVNIYVNSEAIEALQGLGTPLRDGDEVTIIPALAGGRA"
     gene            1723..2211
                     /locus_tag="VLF19_01660"
     CDS             1723..2211
                     /locus_tag="VLF19_01660"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HSE91983.1"
                     /translation="MILAPDELDAIRRQAVEEYPNECCGVVMVRGRDRQVVRCRNIQD
                     ELHALDPVRHPRTARTAFNIGKDDHDRIASLQAQGFELAVIYHSHVDVGAYFSETDRR
                     MAMMGQDPRRHDPLYPDVTHVVMAVTRDGVEQVNAFRWNPTTRDFPPVEPGVTVPSER
                     GA"
     gene            2211..3488
                     /gene="hemL"
                     /locus_tag="VLF19_01665"
     CDS             2211..3488
                     /gene="hemL"
                     /locus_tag="VLF19_01665"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSE91984.1"
                     /translation="MDSDGLFRKAQQVIPGGVNSPVRAFRGVGGEPFFVARALGARLT
                     DVDGRSYVDFVGSWGPLILGHAPPAVVEAVAEAAGRGTSYGAPTAQEVEMAEAVTAAC
                     PSMDLVRLVSSGTEATMSAIRVARGATGRDVIVKFDGCYHGHADSLLVKAGSGGATLG
                     IPDSRGVPDGLARLTATLAFNDLEAVRELFRRRGDDIAAVIVEPVAGNMGVVPPVPGF
                     LEGLREVTARHDSVLIFDEVITGFRVAYGGAQARYGVRPDLTCLGKIIGGGLPVGAYG
                     GRRDLMAHVAPLGGVYQAGTLSGNPLAVAAGLATLRALREGDPYARLEALGARVEAGL
                     RAASDKAGVPLTVNRVGSMLTGFFTDGPVTDYATARRADTARYGRYFHAMLDRGVFLA
                     PSQFEAAFVSLAHTEADIDHAARAATEALAIVG"
     gene            3568..4155
                     /locus_tag="VLF19_01670"
     CDS             3568..4155
                     /locus_tag="VLF19_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSE91985.1"
                     /translation="MSAKVGVGALGLALLAVLIFLAASSWSRRPAVAQPSIQPIAFPH
                     NVHVQQFKIDCQYCHADARRSEYAGLPSVARCMGCHKITLADRPEIKKLADYAARNEP
                     IPWVRVFKVPEFTYFPHKPHVRAGVVCQTCHGPVETMTTVGARTGPRLANDLLILAGV
                     RASPPPLTMGWCVDCHRAENAKGVRAPLDCVTCHH"
     gene            4160..>5333
                     /locus_tag="VLF19_01675"
     CDS             4160..>5333
                     /locus_tag="VLF19_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE91986.1"
                     /translation="MNRRDFFKVVGVCGAAAAAAGCQQPVEQILPLVVPNEQLVPGVA
                     AWFATVCRECPAGCGVLARNREGRVVKLEGNPDHPVNHGALCIRGQAALQGVYHPDRF
                     AGPQRRDGAGWKPLPWDDALKSVAGKIGELRQGGKARAVALVTQLEAGSLGALGDRFT
                     QALGARPRITLEPLGYEWMRAANRAVFGRDAIPYHAFQDAEVVLSFGADFLETWLSNV
                     DHARGFGRMHGFRTGRAGTVIHVEPRQSLTASNADEWVRNAPGTEALVALAVLRALLD
                     EGVVDKRFGEAVAGLDLKKAAEESGVALETIKHVAQVFGHASAVLAVGGGAGVSGTHA
                     VQTQVAVNLLNAAMGAVGKTVLFGPDSAYARVTPYADVAALVGAMGNGEVEVLLLGP"
BASE COUNT          722 a         1883 c         1967 g          761 t
ORIGIN      
        1 gcctactcgg tgtccggcga gtacgcgatg ctcaaggccg ccgcgcagct cggctggctc
       61 gacgaggacc gcgccatgat ggaggcgctc accgggatcc ggcgcgctgg cgccgacatc
      121 gtcatcacgt acttcgccaa ggacgtcgcg cgccgcctgg agcgcaagcg ggagccttga
      181 ggaggcggtc atcggcggtg ggcgccggcg cgacgttcga ggccacgcgc gagcgcggag
      241 ctcgagccga gcgcgacctc gtggagccgt gggatgagat cgccggggcc gtctcgcacg
      301 tcgtccatcg cctcacgctc ggcgaactgt cggcacgcgt gcgcgagcgg ctgtcgctcg
      361 agcgcgcgac gtacgtctga gcatggaccg tccgctcgtc gccgagagcg tgctcgatct
      421 catcggctgg acgccgatgg tgcgactcaa ccggctgccg agcccgaagg gcgcccgcgt
      481 cctcgccaag ctcgagtcgg tcaacccggg cggcagcgtc aaggaccgca tcggcatcgc
      541 gatgctggag gacgccgagc ggcgcggtgc cctccggccc ggaggcacga tcgtggagcc
      601 cacgagcggc aacacgggga tcgggctggc catggcggcg gcggtgaagg gctaccgcct
      661 catcctcacg atgcccgacg acatgagcgt cgagcgccag cggctgctcg cacgcttcgg
      721 cgccgagatc cacctgacgc cggccatcga gggcatgacg ggggcggtgt tcgccgcgca
      781 ggagctgctc cgggagcacc ccgactactt catgccgcag cagttcgaga atcccgcgaa
      841 ccccggggtc caccggcgca ccacggcgct cgagatcctg tcggcggtcg gcgaccggcg
      901 catcgacgcc ttcgtggccg gcgtgggcac gggcggcacg gtgaccggtg tgggcgaggt
      961 gctgaaggag aagatgccgg gcgtgcgcgt tgtcgccgtg gagcccgcgc gctccgcggt
     1021 gctgtccggt ggcagagcgc ggccccacgc gatccagggc atcggcgcgt ccttcgtgcc
     1081 cggggtgctg aaccgcgccg tgctcgacag catcgtgcag gtccgcgacg aggacgccat
     1141 cgcgtggtcg cggcgcctcg cgcgcgagga ggggctgctc gtcggcatct cggcgggtgc
     1201 cgcggtcttc gcggcctgcg gggtcgcggg agatctgtcg cccgagcagc tcgtcgtcac
     1261 cgtgctgccg gataccggcg agcgctacct cagcatgggc tgagcgagat ctccccgcgg
     1321 ggagatggcg ccgaccagcc cgcgctcgag cagagaagct ggtgggctcg cggcccgagt
     1381 gccgggcgcc ggcgctctgc gccgacgcac cgacggcaca tgagatggac ggaggatcag
     1441 ggccatggcg gtgatggtct acattccgac gccgttccgg cgagcgaccg gcaaccgcga
     1501 ccgggtcgag gtcgcggccg gtgacgtcgg cgacctgctg gacgggctcg agcgtcagtt
     1561 cgccggactg cgcggcctcg tgcgcaacga cagcggcgag gtccaccacc acgtcaacat
     1621 ctacgtcaac agcgaggcga tcgaggcgct ccaggggctc ggcaccccgc ttcgcgacgg
     1681 cgacgaggtg accatcatcc cggcgctggc gggtggccgc gcgtgatcct cgcgcccgac
     1741 gagctcgatg cgatccgccg gcaggcggtc gaggagtacc ccaacgagtg ttgcggggtc
     1801 gtcatggtcc gtggccgcga ccggcaggtc gtgcgctgcc gcaacatcca ggacgagctt
     1861 cacgcgctcg atcccgtgcg ccacccgcgg accgcgcgga cggccttcaa catcggcaag
     1921 gacgaccacg accgcatcgc gagcctccag gcccagggct tcgagctcgc ggtcatctac
     1981 cactcgcacg tggacgtggg agcctacttc tcggagacgg accgccgcat ggccatgatg
     2041 gggcaggacc cgcgccggca cgacccgctc taccccgacg tgacgcacgt cgtgatggcg
     2101 gtcacgcgtg acggggtgga gcaggtcaac gcgttccgct ggaacccgac gacgcgggac
     2161 ttcccgccgg tggagcccgg cgtgacggtt ccgagcgagc ggggggcgtg atggactccg
     2221 acggcctctt ccgcaaagcc cagcaggtga tccccggtgg cgtgaacagt ccggtgcgcg
     2281 cgttccgcgg cgtcggcggc gagcccttct tcgtcgcgcg cgcgctcggt gcgcggctga
     2341 cggacgtgga cggtcggtcc tacgtggact tcgtcggctc gtgggggccg ctcatcctcg
     2401 gccatgctcc gcccgccgtc gtcgaggccg tcgcggaggc cgccggtcgg ggcacctcgt
     2461 acggggcgcc gaccgcgcag gaagtggaga tggccgaggc cgtcaccgcc gcctgtccgt
     2521 cgatggatct ggtccggctc gtcagttccg gcaccgaggc gacgatgagc gcgatccgcg
     2581 tggcgcgcgg cgcgaccgga cgcgacgtca tcgtcaagtt cgacgggtgc tatcacggcc
     2641 acgccgacag cctgctggtg aaggccggct ccggcggcgc cacgctgggg attcccgaca
     2701 gccgtggcgt tcccgacggc ctcgcgcgcc tgacggcgac gctggcgttc aacgacctcg
     2761 aggccgtgcg cgagctcttc cggaggcgcg gtgacgacat cgccgcggtg atcgtcgagc
     2821 cggtcgccgg caacatggga gtggttcctc ccgtgccagg cttcctcgag gggctgcgcg
     2881 aggtgacggc gcgccacgac agtgtgctca tcttcgacga ggtgatcacc ggcttccgtg
     2941 tcgcgtacgg cggcgcccag gcccgatacg gcgtgcggcc cgatctgacg tgcctcggca
     3001 agatcatcgg gggtggtctg cccgtgggcg cctacggagg ccggcgggat ctcatggccc
     3061 acgtggcgcc gctcggcggc gtttatcagg ccggcacgct gtcgggcaac ccgctcgccg
     3121 tcgccgccgg gctggcgacg ctgcgcgcgt tgcgggaggg cgatccctac gcgcggctcg
     3181 aggccctcgg cgcccgcgtc gaggccggcc tgcgcgcggc ctccgacaag gccggcgtcc
     3241 cgctgacggt gaaccgcgtg ggctcgatgc tcacgggctt cttcaccgac gggccggtga
     3301 ccgactacgc gacggcccgg cgcgccgaca ctgcgcggta cggacgctac ttccacgcga
     3361 tgctcgatcg cggggtgttc ctcgctccgt cacagttcga ggcggctttc gtctcgctcg
     3421 cccacacgga ggcggacatc gatcacgcgg cacgcgccgc gacggaagcg ctcgccatcg
     3481 tcgggtgatc gactgttgac ttgtctccgg cgctggtgat agcttgcaca agcttcttgc
     3541 gatgccccgg agggaacccg accgtcgatg agcgcgaagg tgggtgtggg cgccctggga
     3601 ctggcgctcc tggccgtgtt gatcttcctg gccgcatcgt cgtggagccg tcgaccggcc
     3661 gtcgcccagc cttcgattca accgatcgcc ttcccccata acgtccacgt ccagcagttc
     3721 aagatcgact gccagtactg tcacgccgac gcacgccgct cggagtacgc gggcctgccc
     3781 tccgtcgccc gctgcatggg ctgccacaag atcacgctcg ccgaccggcc ggagatcaag
     3841 aagctcgccg actacgccgc caggaacgag cccatcccgt gggtgcgcgt cttcaaggtg
     3901 ccggagttca cttactttcc ccacaagccc cacgtgcggg cgggcgtcgt ctgccagacc
     3961 tgtcacggac cggtggagac catgaccacc gtcggcgcgc gcacgggccc gaggctggcg
     4021 aacgacctgc tgatcctcgc cggcgtgcgc gcgtcgccgc cgccgctgac gatgggctgg
     4081 tgcgtcgact gccaccgcgc cgagaacgcg aagggcgtgc gggcgccgct cgactgcgtg
     4141 acgtgccacc actgatccca tgaaccggcg cgacttcttc aaggtcgtgg gggtctgcgg
     4201 ggcggcggcc gcggccgcgg ggtgccagca gcccgtcgag cagatcttgc cgctcgtcgt
     4261 ccccaacgag cagctcgtgc ccggcgtggc cgcgtggttc gccaccgttt gccgcgagtg
     4321 cccggccggc tgcggggtgc tggcccgcaa ccgcgagggc cgggtggtga agctcgaggg
     4381 aaacccggac cacccggtga accacggcgc gctctgcatc cgcgggcagg cggccctcca
     4441 gggtgtctat catcccgacc ggttcgcggg gccgcagcgg cgtgacggcg ccggctggaa
     4501 gccgctgccg tgggacgacg ccctcaagag cgtcgccggc aagatcggcg agctgcgcca
     4561 gggcggcaag gcgcgggcgg tcgcgctcgt cacgcagctc gaggccggca gcctcggcgc
     4621 gctcggcgat cgcttcacgc aggcgctcgg cgcccgcccg cgcatcacgc tggagccgtt
     4681 gggctacgag tggatgcgcg cggccaaccg cgccgtgttc gggcgcgacg ccattcccta
     4741 ccacgcgttc caggacgccg aggtggtgct gtcgttcggg gccgacttcc tggagacgtg
     4801 gctctcgaac gtcgaccacg cgcggggttt cggccgcatg cacggcttcc gcaccggccg
     4861 cgccggcacg gtgatccacg tcgagccccg gcagtcgctc acggcctcca acgccgacga
     4921 atgggtccgg aacgcgccgg gcacggaggc gctcgtggcg ctggcggtgc tccgggcgct
     4981 gctcgacgag ggtgtcgtcg acaagcgctt tggcgaggcg gtggccggcc tcgatctcaa
     5041 gaaggccgcc gaggagagcg gcgtcgccct cgagaccatc aagcacgtcg cccaggtgtt
     5101 cggccacgcc agcgcggtgc tggccgtggg cggcggggcc ggcgtgagcg ggacccacgc
     5161 cgtgcagacg caggtcgccg tcaatctgct caacgcggcc atgggcgccg tgggcaagac
     5221 cgtgctcttc gggccggact cggcctacgc gcgcgtcacg ccgtacgcgg acgtcgccgc
     5281 gctcgtcggc gccatgggca acggcgaggt cgaggtcctg ctgctcgggc ccg
//
LOCUS       DATIAZ010000050         8973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1721527, whole genome shotgun sequence.
ACCESSION   DATIAZ010000050 DATIAZ010000000
VERSION     DATIAZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721527"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VLF19_01680"
     CDS             <1..504
                     /locus_tag="VLF19_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE91987.1"
                     /translation="NVGIGSRGSILDESEESWRRLMQVNVDTMFLAGRHAIPAMKRAG
                     GGAIVNVSSISALRPRGLTAYSVSKGAVIALTRAMAVDHGPDGIRVNCVAPGPVYTPM
                     VYTRGMSAVAREQRRKASVLGLEGTGWDIGHAVRFLLSDHARYITGHTLVVDGGTTLT
                     APERDSR"
     gene            complement(886..1779)
                     /locus_tag="VLF19_01685"
     CDS             complement(886..1779)
                     /locus_tag="VLF19_01685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSE91988.1"
                     /translation="MNRLGWLAFALVLGGCQQATDVARTLPIPGVAGSLVRGGAEALP
                     AARGAFVDMEEPEEIELGRAVTTNIGSRYRLLRDASLTRYVALVGNTVAAHSERPDVK
                     YYFGVLDAPEVNAFAAPGGYVFITVGALRLMKDEAALAGVLGHEVAHVARRHHQEAIK
                     AERRKQIAMIGVREGLAYTPAAAFGGVISMAADKVADQVILKGFSRAEEGDADQAGFR
                     YAAAAGYDPAGLGDFLAALVAGGEKEATATRFFSTHPGTPERLQEQRKLIAARSSADG
                     RRNPERFTQNAGQYLAPPPAR"
     gene            complement(1776..2252)
                     /locus_tag="VLF19_01690"
     CDS             complement(1776..2252)
                     /locus_tag="VLF19_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HSE91989.1"
                     /translation="MRRIVVAVTLVAAVGASAQETSRWVERAKADVRAGRGSYFDVVD
                     TVMKNDELRVLRSEARWLFVRTPKAREGWIFESALAAKPVGGDSELLKLLPGDSATSA
                     TAASAGAKGVYAQAYAREKGFDYGVVQWIETNQGLTAVDHERFLREGALTVPGGAR"
     gene            complement(2304..4502)
                     /locus_tag="VLF19_01695"
     CDS             complement(2304..4502)
                     /locus_tag="VLF19_01695"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HSE91990.1"
                     /translation="MRLRATSLRLLAGGLIGLAATVGAGAAATTVVIDTYERRLLDLR
                     TELFARRERADGRIVAVVIDQKSLDAIAARPDRGGLDHGWPWPRDYYAAVVAYLFAAG
                     ARAVGIDLVLSEPSVYTRLGVAEDDAALAEAIEAEPVVLAAMLTRERAGAASLADVAW
                     PAVLREQGKAREVRGLVPDLNRASLPVEPLAGAARWLGWIGFEPDVDGVCRSIRPAAG
                     YAPQGSDTAVEVPAFAVALAEAAGADVQTRPRRPAGERLVVDGRLIPLDEDGRILLRF
                     HGGERTYRDFSFASVLRSAKRHAAGLPVDDASPADFRGKIVIVGANAAGLLDLRATPV
                     GPALPGYAIHATALDNLLAGNPLSRLPRATRAALVTLAGPLCGVLVAAAARPRESGLA
                     ALALALAHTAGALWAFAARGVWVDLLPPLMALGLAYAGTSGYLYVTEGRRQRFLRDAF
                     SRYLSPAFVERLVTDPRRLALGGESRPVTVLFADVVGFTTLSEGREPAELVRLMNECF
                     TELTDVIQARGGTVDKFIGDAIMAFWNAPADLPDHPRRAALAAREMLAALDALRARWL
                     AEGRPAVDMRIGLATGPALVGNVGSRSKFNYTAMGDTVNLASRLEGAAKVYHTRTLVA
                     ASTATAADGVRFRELDWLQVKGRSEPVPVFEILPADVDITREDVHRHYAEGLAAYRAR
                     RFDDALARFEKALATDPDDGPSGEMHARCIEYLKQPPPPEWRGEHVLTSK"
     gene            complement(4599..6314)
                     /locus_tag="VLF19_01700"
     CDS             complement(4599..6314)
                     /locus_tag="VLF19_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE91991.1"
                     /translation="MPHDLVIKNGVLIDGSGLARYRADVAVKHGRIVSIGRVREGARE
                     VIDADGMVVAPGFVDGHTHMDAQIFWDPLGTCSCWHGVTTVVMGNCGFTLAPCAEDKK
                     HLVVRNLERAEDISAEAMAAGITWQWTTFPEWLDCLDGLPKGINYASYLGHSALRTHV
                     MGERAFEHAASEHELRAMERELRDALRAGAMGFTTSRSPIHQTPDGRPVASRLAGWDE
                     IRRLVGVMGDLNAGIFELAGEAAGRELTDPVAFREYHVRLRDLAVETGRPVTFGTFSR
                     LEAPDVWRHYMNLLDETAAAGGRMFAQVHSRGLNVVLSFKTQLPFDDLPAWKPFRALP
                     LAEQRERLRDPAVKRRLVDGAHDEPDRKTLGTEARPFPYEWIFLYDTVAGPHPTITDI
                     AHERGVDPAEAMIDLALERDLDRFFIHPVANEQQDTVLEMMRHPRAVVTFSDSGAHVS
                     QIIDSSLQTHLLSHWVREQQAFTLEQAVRMLTLVPATYWGFADRGLVREGFAADLIVF
                     DPDTIAPEMPEVVHDLPAGARRLVQRARGIAATVVNGEVLLRDGKHTGALSGRLLRGP
                     TARTA"
     gene            complement(6506..7675)
                     /locus_tag="VLF19_01705"
     CDS             complement(6506..7675)
                     /locus_tag="VLF19_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HSE91992.1"
                     /translation="MRTSDERILTTHVGSLPRPPALRDLLVKQDRGEAIDERELDRQV
                     EAAVRDVVARQQAAGIDVGNDGEQPRVGFSTYVARRMQGFGGESKRPPARDAVEFPDY
                     AAMLEYRRRAAARIANAPQAVAEVRYDDLSEAARECDVFARCTGGSPPPFVERFMTAA
                     SPGTIATVLMNAHYDSHERYVMALAREMRKEYDLIHARGFVLQLDCPDLAMERARFFQ
                     HESVEGFQRAVALHVEAINQATAGIPADRIRLHVCWGNYDGPHIHDVPLEAILPIVLR
                     ARVGALSLPLANPRHQHEHRLIARHGVPAGMLFLPGVIDTTTNYVEHPEVVADRIVQA
                     VEAVGDRTRVIASTDCGFGTFAGSENCAPSVVWAKLRALREGADLATRRLDRRGE"
     gene            complement(7682..8194)
                     /locus_tag="VLF19_01710"
     CDS             complement(7682..8194)
                     /locus_tag="VLF19_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HSE91993.1"
                     /translation="MTGLAALAAPLAALLVERKETLAVAESSAGGLISAALLAVPGAS
                     AYFLGGGVIYTQGARRALLQVPDESVKGIRSSTEAYALVKARTIRERLGATWGLAETG
                     ASGPSGNRYGDAPGHACLAVAGSIERALTLETGHGDREKNMWAFTRAALELLEQSIRA
                     AGPPRAGHGR"
     gene            complement(8191..>8973)
                     /locus_tag="VLF19_01715"
     CDS             complement(8191..>8973)
                     /locus_tag="VLF19_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HSE91994.1"
                     /translation="LRTHGITVAPDGTVWCTDDGNHTVRRFTPEGKLLMTLGTMNTPS
                     DTGYDGKDTMSVKRAAGPFNRPTNIAVGPKGDLYVSDGYGNSRVHQFSPTGALKRSWG
                     EPGTGPGQFYLPHGIAVAADGRVFVCDRESDRIQIFSPDGEYLSEWVDTQRPTHLVFD
                     AQGRAYVSELWWYLGQTSRRHGKMERPTFGRVSVYDREGRMLARWGKPDMASPGGLVG
                     PHGVAVDSRGDVYVSEVTWTFGVSRGHAPEGTHTFQKFALAR"
BASE COUNT         1205 a         3259 c         3289 g         1220 t
ORIGIN      
        1 aacgtcggca tcggcagccg cggctccatc ctggacgaga gcgaggagag ctggcgccgg
       61 ctgatgcagg tgaacgtgga cacgatgttc ctggctgggc ggcacgcgat cccggcgatg
      121 aagcgcgctg gcggcggcgc catcgtcaac gtctcgtcga tctccgcact gcgcccccgc
      181 ggcctcacgg cctattcggt ctccaagggc gccgtcatcg cgctcacccg ggcgatggcc
      241 gtggaccacg gccccgacgg gatccgcgtc aactgcgtgg cgcccgggcc cgtctacacg
      301 ccgatggtct acacgcgggg catgagcgcg gtggcgcgcg agcagaggcg caaggcgtcc
      361 gtcctcggcc tcgagggcac cggctgggac atcggccacg cggtgcgctt cctgctctcg
      421 gaccacgccc gctacatcac cggtcacacg ctcgtggtgg acggcggcac gacactgacg
      481 gcgcccgagc gcgactcccg gtgagcgcga cggtcgacag tcctggcggc cgaaggtaga
      541 ctctaccggc gatcgcgtcg agcggcacgg tcacacgctg cacgaaacgc acagcaaggc
      601 cgtgtcgcca gcgctcccgg cgacccccct ctacgtcgac gccgatcccg cgcggctcga
      661 gcagatcatc gtcaacgtcc tcaacaatgc cgccaagtac acgcccgctc gacggccgga
      721 tcagcgccga gccgatcctc gtcgccatca gcgggtacgg gcaggcccac gaccggcgtc
      781 gctcggtcga ggccggcttc gacgcgcacc tgacgaagcc ggtctctctc gaccatctct
      841 cgggcctcct tgccgcgctg gcccgcggcc gcgagccggg tgggcctacc gggccggcgg
      901 cggcgccagg tattgccccg cgttctgcgt gaagcgctcc gggttgcgtc ggccgtccgc
      961 gctcgaccgc gcggcgatca gcttccgctg ctcctgcagc cgctcgggcg tgccggggtg
     1021 tgtcgagaag aaccgggtgg cggtcgcctc cttctcgccg ccggcgacca gcgccgcgag
     1081 gaagtcgccc aggccggccg gatcgtagcc cgccgcggca gcgtagcgga agccggcctg
     1141 gtcggcgtcg ccctcctcgg cgcggctgaa gcccttgaga atcacctggt ccgccacctt
     1201 gtcggcggcc atgctgatca cgccgccgaa ggcggcggcc ggcgtgtacg ccaggccctc
     1261 gcgcacgccg atcatggcga tctgcttcct ccgctcggcc ttgatcgcct cctggtgatg
     1321 gcgtcgcgcg acgtgcgcca cctcgtggcc caggacgccg gccagtgcgg cctcgtcctt
     1381 catcagccgg agcgcgccca cggtgatgaa gacgtaaccg cccggcgccg cgaacgcgtt
     1441 cacctccggc gcgtcgagca cgccgaagta gtacttcacg tcgggccgct ccgagtgcgc
     1501 ggccacggtg ttgccgacca gcgccacgta gcgggtcagg ctggcgtcgc gcaagagccg
     1561 gtagcgcgag ccgatgttcg tcgtcaccgc gcgacccagc tcgatctctt caggctcctc
     1621 catgtcgacg aaggcacccc gggccgcggg cagcgcctcg gcgcccccgc gtacgaggga
     1681 accggcgacg ccggggatgg gcagcgtgcg ggcaacgtcg gtcgcctgct ggcagccgcc
     1741 gagcacgagc gcgaaggcga gccacccgag cctgttcatc gcgcgcctcc cggcacggtc
     1801 agggcaccct cgcggaggaa ccgttcgtgg tcgaccgccg tcaggccctg gttggtctcg
     1861 atccactgga cgacgccgta gtcgaagccc ttctccctgg cgtaggcctg ggcgtagacg
     1921 cccttggcgc ccgcggatgc cgcggtggcg gaggtcgccg agtctccggg caggagcttc
     1981 agcagctccg agtctcctcc cacgggcttg gcggcgaggg cgctctcgaa gatccacccc
     2041 tccctggcct tcggcgtccg cacgaacagc cagcgggcct cgctccggag gactcggagc
     2101 tcgtcgttct tcatgacggt gtcgacgacg tcgaagtacg agccgcgccc ggcccgcacg
     2161 tcggccttcg cgcgctcgac ccagcgggag gtttcctgcg ccgacgcgcc gacggccgcg
     2221 accagtgtga ccgcaacgac gatccgcctc atgctgtccc ccctcgttcg tggttcgccc
     2281 gtgacgacag tgcgacgcgc gcgtcacttg ctcgtgagca cgtgctcgcc ccgccactcc
     2341 gggggcggtg gctgcttcag atactcgatg cagcgggcat gcatctcgcc cgatggcccg
     2401 tcgtccgggt ccgtggccag cgccttctcg aaccgcgcca gggcgtcgtc gaagcgacgg
     2461 gcgcggtacg cggcgagccc ctcggcgtag tggcgatgga cgtcctcccg agtgatatcc
     2521 acgtccgcag gcaggatctc gaagaccggc accggctcgc tccgtccttt gacctgcagc
     2581 cagtcgagct cccggaagcg caccccgtcg gccgccgtcg ccgtgctcgc ggcgacgagc
     2641 gtgcgcgtgt ggtagacctt ggccgcgcct tcgagcctgg aggcgaggtt caccgtgtcg
     2701 cccatggccg tgtagttgaa cttgctccgc gacccgacgt tgccgaccag cgccgggccg
     2761 gtggccagcc cgatccgcat gtcgacggct ggccggccct ccgcgagcca gcgagcgcgc
     2821 agcgcatcga gggcggccag catctcgcgg gcggcgagcg ccgcgcggcg cgggtgatcg
     2881 ggcaggtcgg cgggcgcgtt ccagaacgcc atgatggcgt cgccgatgaa cttgtcgacg
     2941 gtgccgccgc gggcctggat cacgtcggtc agctcggtga agcactcgtt catcagtcgg
     3001 accagctcgg caggctcccg gccctcagac agcgtggtga agccgacgac gtcggcgaag
     3061 agcacggtga ccggccggct ctcgccgccg agcgcgaggc ggcgcggatc ggtcacgagc
     3121 cgctccacga aggccggcga gaggtagcgc gagaaggcgt cgcgcaggaa ccgctggcgg
     3181 cggccctcgg tcacgtagag gtagccgctc gtgcccgcgt aggcgagacc cagcgccatc
     3241 agcggcggca gcaggtcgac ccacacgccg cgcgcggcga aggcccacag cgcgccggca
     3301 gtgtgcgcga gcgcgagggc gagggcggcc agcccgctct cgcgcgggcg ggcggccgcg
     3361 gcgacgagca cgccgcacaa cgggccggcg agcgtcacga gggcggcgcg cgtggcccgc
     3421 gggagccgcg acagcgggtt gccggcgagc aaattgtcga gggccgtggc gtggatcgcg
     3481 tagccgggca aggcggggcc cacgggcgtt gcgcgcagat cgagcaggcc cgccgcgttg
     3541 gcgccgacga tcacgatctt gcccctgaag tcggccgggc tcgcgtcgtc caccggcaga
     3601 ccggcggcgt ggcgcttggc ggaccgcagc accgaggcga aggaaaagtc gcggtaggtc
     3661 cgctcgccgc cgtggaagcg gagcaggatg cggccgtcct cgtccaaggg gatcagccgg
     3721 ccgtcgacga ccaggcgctc gccggcaggc cgccggggcc ttgtctgcac gtcggcgccc
     3781 gcggcctctg ccagcgccac ggcgaacgcg gggacctcga cggcggtgtc gcttccctgg
     3841 ggcgcatagc cggcggcggg ccggatggag cggcacacgc cgtccacgtc gggctcgaag
     3901 ccgatccagc cgagccaccg cgcggcgccc gcgagcggct cgacgggcaa cgacgcgcgg
     3961 ttcaggtccg gcacgagccc ccggacctcc cgcgcctttc cctgctcgcg gagcaccgcg
     4021 ggccacgcga cgtcggcgag cgaggcggct ccggcgcgct cgcgggtgag catggccgcg
     4081 aggacgacgg gttcggcctc gatggcctcc gccagcgccg cgtcgtcctc cgcgaccccg
     4141 agccgcgtgt agacgctcgg ctcgctgagc acgaggtcga tcccgaccgc gcgcgcgccg
     4201 gccgcgaaga gataggcgac gacggccgcg tagtagtcgc gcggccacgg ccagccgtga
     4261 tcgagtccgc cccgatcggg ccgcgcggcg atcgcgtcga gcgacttctg gtcgatcacg
     4321 accgcgacga tgcggccgtc ggcgcgctcg cggcgggcga agagctcggt gcggagatcg
     4381 aggaggcgcc gctcgtacgt gtcgatcacc accgtggtcg ccgcggcgcc ggcgccgacg
     4441 gtcgcggcga ggccgatgag gccgccggcg agcagacgca gcgacgtggc acgaagcctc
     4501 acggatcgaa catagcagac cctgtcgcct ccgggcagcc gccggtgacg cggcggtgcg
     4561 ccgccgccgg ccggccacct tgcccgagcg agggagggtt acgcggtccg cgccgtcggt
     4621 ccccgcagca gccggccgga cagggcgccg gtgtgcttgc cgtcgcgcag caggacctct
     4681 ccgttgacga ccgtcgccgc gatgccgcgc gcccgctgca cgagccggcg ggcgccggcg
     4741 ggcaggtcgt gcacgacctc cggcatctcg ggcgcgatcg tgtcggggtc gaagacgatc
     4801 aggtcggcgg cgaagccctc gcgcacgaga ccgcggtcgg cgaagcccca gtacgtcgcg
     4861 ggcaccagcg tcaacatgcg tacggcctgc tcgagcgtga acgcctgctg ctcgcgcacc
     4921 cagtggctca gcaggtgggt ctgcagcgag gagtcgatga tctgggagac gtgagcgccg
     4981 gagtccgaga aggtcaccac cgcgcgcggg tggcgcatca tctccagcac ggtgtcctgc
     5041 tgctcgttgg cgaccgggtg gatgaagaag cggtcgaggt cgcgctcgag ggccaggtcg
     5101 atcatcgcct cggccgggtc cacgccccgc tcgtgcgcga tgtcggtgat cgtgggatgc
     5161 ggccccgcga ccgtgtcgta gaggaagatc cactcgtacg ggaagggccg ggcctcggtg
     5221 ccgagcgtct tgcggtcggg ctcgtcgtgg gcgccgtcga ccagccgccg cttgaccgcc
     5281 ggatcgcgca ggcgctcgcg ctgctcggcc agcggcaggg cccggaacgg cttccacgcc
     5341 ggcaggtcgt cgaagggcag ctgggtcttg aacgacagca cgacgttgag gccgcggctg
     5401 tggacctggg cgaacatccg gccgccggcg gccgccgtct cgtcgaggag gttcatgtag
     5461 tggcgccaca cgtcgggcgc ctccagccgg ctgaaggtgc cgaacgtcac cgggcgcccg
     5521 gtctcgacgg ccagatcgcg caggcgcacg tggtactcgc ggaaggcgac cgggtccgtg
     5581 agctcgcggc cggcggcctc gccggccagc tcgaagatgc cggcgttcag gtcgcccatc
     5641 acccccacga gccgccggat ctcgtcccac cccgcgagac ggctcgccac cggccggccg
     5701 tcgggcgtct ggtggatcgg cgagcgcgag gtcgtgaagc ccatcgctcc cgcgcggagg
     5761 gcgtcgcgca gctcgcgctc catcgcgcgc agctcgtgct cgctcgccgc gtgctcgaag
     5821 gcgcgctcgc ccatcacgtg cgtgcgcagg gcggagtggc cgagatagct cgcgtagttg
     5881 atgcccttgg gcagcccgtc gagacagtcg agccactccg ggaaggtcgt ccactgccag
     5941 gtgatgcccg cggccatggc ctcggcggag atgtcctcgg cccgctcgag attgcgcacg
     6001 accaggtgct tcttgtcctc ggcgcacggc gccagcgtga agccgcagtt gcccatgacc
     6061 acggtggtga cgccgtgcca gcacgagcag gtgcccagcg ggtcccagaa gatctgggcg
     6121 tccatgtggg tatggccgtc gacgaagccg ggggccacga ccatgccgtc ggcgtcgatc
     6181 acctcgcgcg cgccctcgcg gacgcggcca atggagacga tgcgaccgtg cttcacggcg
     6241 acgtcggccc ggtagcgggc caggccggag ccgtcgatca gcacgccgtt cttgatcacg
     6301 aggtcatggg gcatcgagca ccctccttgc tggtccctgg gatcgcgcgt gggcggcact
     6361 attctaccgg tccccgcccg gagagacacg cggcgacccc cgggaacctc gagccgagcg
     6421 taacggcggc gcgactgacg acggcgcctt ccggtgcgca caatcggccc cacgcaggcg
     6481 acgatgcgtc gccgacgcgc ccggctcact cgcctcgccg gtcgaggcgg cgcgtcgcca
     6541 ggtcggcccc ttccctcagc gcgcggagct tggcccagac cacgctcggc gcgcagttct
     6601 cggagccggc gaacgtcccg aacccgcagt cggtcgaagc gatgacccgg gtgcggtcgc
     6661 cgaccgcctc gacggcctgg acgatgcggt cggcgacgac ctcggggtgc tccacgtagt
     6721 tggtggtcgt gtcgatcacg cccggcagga agagcatgcc ggccggcacg ccgtggcggg
     6781 cgatcagccg gtgctcgtgc tggtggcgcg ggttggcgag cggcagcgag agcgcgccca
     6841 ccctggcgcg cagcacgatc ggcaggatcg cctcgagcgg cacgtcgtgg atgtggggtc
     6901 cgtcgtagtt gccccagcag acgtgcaggc gaatgcggtc ggcggggatg cctgccgtcg
     6961 cctggttgat ggcctcgacg tgcagcgcca ccgcgcgctg gaagccctcc acggactcgt
     7021 gctggaagaa gcgggcccgc tccatggcga ggtccgggca gtcgagctgc agcacgaatc
     7081 cccgcgcgtg gatgaggtcg tactcctttc gcatctcgcg ggccagcgcc atgacgtagc
     7141 gctcgtggga gtcgtagtgg gcgttcatga ggacggtcgc gatggtgccc ggcgaggccg
     7201 cggtcatgaa gcgctcgacg aacggcggcg gcgatccccc ggtgcatcgt gcgaacacgt
     7261 cgcactcgcg cgcggcctcg ctcaggtcgt cgtagcggac ctcggcgacc gcctgcggcg
     7321 cgttggcgat gcgcgccgcc gcccggcggc ggtactcgag catcgcggcg tagtccggga
     7381 actccacggc gtcgcgcgcg ggcggtcgct tgctctcgcc gccgaagccc tgcatgcgcc
     7441 gcgcgacgta cgtcgagaac cccacgcgcg gctgctcacc gtcgttgccg acgtcgatgc
     7501 cggccgcctg ctggcgcgcc accacgtcgc gcacggcggc ctccacctgg cggtccagct
     7561 cgcgctcgtc gatcgcctcg ccgcgatcct gcttgacgag gaggtcgcgg agcgcggggg
     7621 ggcgcgggag actcccgacg tgggtcgtga ggatgcgctc gtcactcgtg cgcacgggtc
     7681 ctcagcggcc gtgaccggca cgcggcggcc cggccgcccg gatcgactgc tccagcagct
     7741 ccagcgcggc ccgcgtgaag gcccacatgt tcttctcccg gtcgccgtga ccggtctcga
     7801 gcgtcagcgc gcgctcgatc gagcccgcga ccgcgaggca ggcgtggccg ggcgcgtcac
     7861 cgtagcggtt gccgctgggc ccgctggcgc cggtctcggc cagcccccac gtcgcgccca
     7921 gccgctcgcg gatcgtccgc gccttgacca gcgcgtaggc ctccgtgctc gaccggatgc
     7981 ccttgaccga ctcgtcgggg acctgcagca gcgcgcgccg cgccccctgc gtgtagatca
     8041 cgcccccgcc caggaagtac gcggaggcgc cgggcacggc gagcagcgcg gcggagatga
     8101 gcccgcccgc cgacgactcg gccaccgcga gcgtctcctt gcgctcgacc aggagcgcgg
     8161 cgaggggcgc ggccagcgcc gccagccccg tcatcgcgcc agcgcgaact tctggaacgt
     8221 gtgggtgccc tcgggcgcgt ggccgcggct cacgccgaag gtccaggtga cctcgctcac
     8281 gtagacgtcg ccccgcgaat cgacggcgac gccgtgcggg ccgacgaggc cgccgggcga
     8341 cgccatgtcg ggcttgcccc agcgcgccag catccggccc tcgcggtcgt agacgctgac
     8401 gcggccgaac gtcggccgct ccatcttgcc gtggcggcgc gacgtctgac ccagatacca
     8461 ccagagctcg gagacgtagg cgcgtccctg ggcgtcgaag accaggtggg tcgggcgctg
     8521 ggtgtcgacc cactcgctca ggtactcgcc gtcggggctg aagatctgga tgcggtcgct
     8581 ctcgcggtcg cagacgaaca cgcggccgtc cgcggcgacg gcgatgccgt gcgggagata
     8641 gaactgcccc ggcccggtgc cgggctcgcc ccaggagcgc ttcagcgccc ccgtcggcga
     8701 gaactggtgc acgcggctgt tcccgtatcc gtcggagacg tagagatcgc ccttcggccc
     8761 caccgcgatg ttcgtcggcc ggttgaacgg cccggccgcg cgcttgacgg acatggtgtc
     8821 cttgccgtcg tagccggtgt cggagggcgt gttcatcgtg ccgagcgtca tcaacagctt
     8881 gccctccggc gtgaagcgcc ggacggtgtg attgccgtcg tcggtgcacc acacggtgcc
     8941 gtcgggcgcg acggtgatgc cgtgcgtgcg cag
//
LOCUS       DATIAZ010000051         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_932072, whole genome shotgun sequence.
ACCESSION   DATIAZ010000051 DATIAZ010000000
VERSION     DATIAZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932072"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VLF19_01720"
     CDS             <1..597
                     /locus_tag="VLF19_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HSE91995.1"
                     /translation="PFILGPAAVAAVLFLVGHRGLATPFALASLASLGFFRDPERAVP
                     VTIDGVLAPADGTVTAVVDDEDGFVGPAVRVSIFLSPLDVHVNRAPIAGVVVDARYTP
                     GRFLAAYQPEAGLVNERCAIHIQGERARVTVVQIAGVLARRIVCRVGAGDKLEAGERF
                     GLIRFGSRTDCHMPRGTDVRVRVGEHVTGGVTELARFV"
     gene            594..1382
                     /gene="pssA"
                     /locus_tag="VLF19_01725"
     CDS             594..1382
                     /gene="pssA"
                     /locus_tag="VLF19_01725"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HSE91996.1"
                     /translation="MRRPGRGGRRRRWQELRDKRRRSIFLLPSLLTTGNLFCGFFAIL
                     LAARGRFEEAALAIFVAMVMDLLDGRVARLMKATSQFGVEFDSLADVVSFCAAPAFLI
                     YAFALQDLGRPAWFGAFLFVICGALRLARFNVQTGLGDRRFFVGLSTPAAAGIVASSV
                     LLLYGAPVTRPLRVGLAMATYVVALLMVSTFRYWSFKDLDWARRRPVQTLLIVVLGVM
                     IVATHHEVFLFLIFAGYAVSGPIRWLIVGRPAPAAAEPGTREAT"
     gene            1411..2955
                     /locus_tag="VLF19_01730"
     CDS             1411..2955
                     /locus_tag="VLF19_01730"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSE91997.1"
                     /translation="MDRVIIFDTTLRDGEQAPGFSMNTMEKLEMARQLARLQVDVIEA
                     GFPISSEEDFEATREVARQVGTLEGAPSICGLSRVGLADIDRCWEAVKYARRPRVHTF
                     VATSDIHLKYKLRKSRAEVMKAAVEAVRHARGYCEDVEFSPEDASRSDFDYMCDVLSA
                     VIGAGARTINIPDTVGYAIPKEWGERIARIRERVHGIDDVVLSVHCHNDLGQAVANSL
                     TAIMNGARQVECTINGIGERAGNASLEEIVMALRTRKDFFGVETRVRSEEIFRASRLL
                     SHITGVHVQPNKAVVGENAFAHEAGIHQDGVLKEKLTYEIMKPEDIGRPSNKLVLGKH
                     SGRHALAARLQDLGFALEGTELDRAFRKFKDLADRKKEVYDEDLLAIVTDEATKIPET
                     FELDYLHVISGTGVVPSATVRLRKEGQLFQDSGVGDGPVDAVLAAIDAITGLKGRLQD
                     YAIRAATSGKDAIGEVSVKVDFDGTIVSGKGSSTDVIEASARAYLYALNRMVHANTQR
                     TKEVGP"
     gene            3068..>3577
                     /locus_tag="VLF19_01735"
     CDS             3068..>3577
                     /locus_tag="VLF19_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HSE91998.1"
                     /translation="MTTWRIAVLAGDGIGQEVTPEAVRALQAVAKAGGFGLQLEQALI
                     GGGAIDATGTPLPPATLALCKSAHAILFGAVGGPKWDHAPQERKPERGLLALRKELDL
                     YANLRPARCFPMLVDASPLKREVVEGTDIMVIRELTGGLYFGEPRGIERFADGGARGV
                     NTMVYTTREI"
BASE COUNT          512 a         1197 c         1321 g          547 t
ORIGIN      
        1 ccgttcatcc tcggcccggc agcggtggcc gccgtgctgt tcctggtcgg ccaccgaggg
       61 ctggccacgc cgttcgcgct cgcgtcgctg gcctcgctcg ggttcttccg tgacccggag
      121 cgcgcggtcc cggtcactat cgacggcgtc ctggcgccgg cggacggaac cgtcaccgcg
      181 gtcgtggacg acgaggacgg cttcgtcggg cccgccgtgc gcgtgtcgat ctttctctcc
      241 ccgctcgacg tgcacgtgaa ccgggcgccc atcgccggcg tggtggtgga cgcgcggtac
      301 acgcccggac gcttcctggc cgcctaccag ccggaggccg ggctggtgaa cgagcgctgc
      361 gcgatccaca tccagggcga gcgagcccgg gtgaccgtgg tgcagatcgc cggcgtcctg
      421 gcgcggcgga tcgtctgccg ggtgggcgcg ggcgacaagc tcgaggccgg cgagcgcttc
      481 gggctcatcc gcttcggctc gcgcaccgac tgccacatgc cgcggggcac cgatgtgcgt
      541 gtccgcgttg gcgagcacgt caccggcggc gtcaccgagc tggcgaggtt cgtgtgagac
      601 ggccggggcg gggcggccgg cggcggcgct ggcaggagct gcgcgacaag cgccggcgga
      661 gcatcttctt gctcccgagc ctgctcacca cgggcaacct cttctgcggc ttcttcgcga
      721 tcctcctcgc cgcgcgcggg cggttcgagg aagccgcgct ggcgatcttc gtggccatgg
      781 tcatggatct gctcgatggc cgcgtggcgc gcctgatgaa ggccacgagc cagttcggcg
      841 tggagttcga ctcgctggcc gacgtcgtgt ccttctgcgc cgcgcccgcg ttcctgatct
      901 acgccttcgc gctgcaagat ctcgggcgcc cggcgtggtt cggagcgttc ctgttcgtca
      961 tctgcggcgc gctgcggctc gcgcgcttca acgtgcagac gggcctcggc gaccgccgct
     1021 tcttcgtcgg cctcagcacg ccggccgcgg ccggcatcgt ggcctccagc gtgctgttgc
     1081 tgtacggcgc gccggtgaca cggcccctcc gggtgggact ggcgatggcg acttacgtcg
     1141 tggccctgct gatggtctcg accttccgct actggagctt caaggacctg gactgggcgc
     1201 gccggcggcc ggtgcagacg ctgctgatcg tcgtgctcgg cgtgatgatc gtggccaccc
     1261 accacgaggt cttcctcttc ttgatattcg ccggctacgc cgtgtcggga ccgatccggt
     1321 ggctgatcgt cggccgcccg gcgccggccg cggccgagcc cggcacccgc gaggcgacct
     1381 gagtacgacg acctcgtcga aggaggacgt atggaccgtg tgatcatctt cgatacgacg
     1441 cttcgggacg gcgagcaggc gcccggcttc tccatgaaca ccatggagaa gctggagatg
     1501 gcgcggcagc tcgcccgact gcaggtggac gtcatcgagg cggggttccc catctcctcg
     1561 gaggaggact tcgaggccac gcgcgaggtg gcgcggcagg tcgggacgct ggagggcgcg
     1621 ccgtcgatct gcgggctgtc ccgggtgggg ctcgccgaca tcgaccgctg ctgggaggcc
     1681 gtgaagtacg cgcggaggcc gcgggtccac accttcgtgg cgacctccga catccacctg
     1741 aagtacaagc tccgcaagtc gcgggccgag gtgatgaagg cggcggtgga ggccgtgcgt
     1801 cacgcgcgag ggtactgcga ggacgtggag ttctcgccgg aggacgcctc gcgctcggac
     1861 ttcgactaca tgtgcgacgt gctctcggcg gtcatcggcg ctggcgcgcg gacgatcaac
     1921 atccccgaca cggtgggtta cgcgatcccc aaggagtggg gcgagcgcat cgcccggatc
     1981 cgcgagcggg tgcacggcat cgacgacgtc gtcctctccg tgcactgtca caacgacctc
     2041 gggcaggccg tggccaactc gctgacggcc atcatgaacg gcgcccgcca ggtggagtgc
     2101 accatcaacg gcatcggcga gcgggccggc aatgcctcgc tcgaggagat cgtgatggcg
     2161 ctcaggacgc ggaaggactt cttcggcgtc gagacccggg tgcggagcga ggagatcttc
     2221 cgggcgtcgc ggctgctgtc gcacatcacc ggcgtccacg tgcagcccaa caaggccgtg
     2281 gtgggcgaga acgccttcgc ccacgaggcg ggcatccacc aggacggcgt cctcaaggag
     2341 aagctgacct acgagatcat gaagcccgag gacatcgggc ggccgtcgaa caagctcgtg
     2401 ctcggcaagc actcgggccg gcacgcgctc gccgcgcggc tccaggacct cgggttcgcg
     2461 ctggagggga ccgagctgga ccgcgccttc cggaagttca aggacctcgc cgaccggaag
     2521 aaggaggtgt acgacgagga cctgctggcc atcgtcaccg acgaggcgac gaagatcccc
     2581 gagacgttcg agctggatta cctgcacgtg atcagcggca cgggcgtcgt gccgtcggcg
     2641 acggtacggc tgcgcaagga gggtcagctg ttccaggact ccggcgtggg cgacgggccc
     2701 gtggacgcgg tgctggcggc catcgacgcg atcacggggc tcaaggggcg gctccaggac
     2761 tacgcgatcc gggcggccac gtccgggaag gacgccatcg gcgaggtctc ggtgaaggtg
     2821 gacttcgacg gcacgatcgt gtcgggcaag ggctcctcca ccgacgtcat cgaggccagc
     2881 gcccgtgcgt atctctatgc cctcaaccgc atggtgcacg cgaacacgca gcgcacgaag
     2941 gaggtcgggc cgtagcctcg gagggtcgcg aagccagcgg acgaaaggcg gccgttcgag
     3001 gcggtgtgtg ccgtcggcgg gaggaaggcg acgcgatgtc gctgggaaag gaagggtgcg
     3061 tagcgcgatg acgacctggc ggatcgcggt gctggccggg gacgggatcg ggcaggaggt
     3121 gacgccggag gccgtccggg cgttgcaggc ggttgccaag gcgggcggat tcgggctgca
     3181 gctcgagcag gcgctgatcg gcgggggggc catcgatgcc actggaaccc ccctgccgcc
     3241 cgccacgctg gcgctgtgca agagtgccca cgccatcctg ttcggcgccg tcggtggccc
     3301 caagtgggac cacgccccgc aggagcgcaa gcccgagcgc gggctgctgg cgctccgcaa
     3361 ggagctcgac ctctacgcca acctgcgtcc tgcgcgctgc ttcccgatgc tcgtcgacgc
     3421 ctcgcccctc aagcgtgagg tggtcgaggg caccgacatc atggtcatcc gcgagctgac
     3481 cggcgggctc tacttcggcg agccgcgcgg catcgagcgc ttcgccgacg gcggcgcccg
     3541 cggcgtcaac accatggtct acacgacccg ggagatc
//
LOCUS       DATIAZ010000052         7667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_669475, whole genome shotgun sequence.
ACCESSION   DATIAZ010000052 DATIAZ010000000
VERSION     DATIAZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669475"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1500
                     /locus_tag="VLF19_01740"
     CDS             280..1500
                     /locus_tag="VLF19_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE91999.1"
                     /translation="MELPGGLRALNHRDFRVYYAGQLVALTGTWMQIVAQSWLVLQLT
                     ASPLRLGLIGTLQFAPLLLFSLFTGAVADRLPKRRLLVATQSTQACLALGLAVLIGTG
                     RVEYWHVAVGAVLWGVANALDQPTRQSFVVSLVGRADLISAVGLNSAAFNAARIVGPA
                     IGGLLIARAGIALAFALNTLAFGIAITALLTVGARGAAPPRGGTTMLEEIREGVAYAV
                     RSARVRLVLGLLAVTSFCVFNFSVYVPLLAREVLGLGSEGFGFLMAALGVGAVAAGLA
                     LGWIPSGAPPLAAMKASLATALAGLVALSAVDRFWLAALLLAVVGFAGTITTASCNTS
                     LQLTAPDQLRGRVMSVYTLLSGGVFPLGAFWVGAVSEARGVSAAFLVNGALGLAALGA
                     LVAWWRTRRRRSGG"
     gene            complement(1515..3557)
                     /locus_tag="VLF19_01745"
     CDS             complement(1515..3557)
                     /locus_tag="VLF19_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE92000.1"
                     /translation="MGTLTARVTKASVCPLDCPDTCSLAVTVENDTIVDIRGTRANPY
                     TEGVLCAKVPASYPEFVHGPGRLRTPLRRVGAKGGGRFARISWDEALDQIHDRFTAVM
                     AEHGRQAILPLNYAGPHGFLAGGSMDLRFFHRLGASLLDRKPLCGGIRTESWIGTYGS
                     VPGIRPEQVEDARLIVVWGNNVTWCNLHLTPIIHRARRKGARLVVVDPRRTKIAEQAD
                     LHLALRPGTDVLLAFAVTGELERLGALDREFIARHVHGFDGYMERVRPWPVATAAPLC
                     GVPEAQIRTLAEWYATLSPAAISVGNGLERNQNGGSGIRAVFALPALAGKWGVRGGGL
                     VNGARLAFPTTPQRLARPDLIPPGTRTLNIIDVGAHLLDARLKPPVGAVFIYNHNPLV
                     VHPDQNTLRRGLAREDLFVVGCDVVMTDSLAYADVVLPACSHFEHADLFAAYGQHWLQ
                     RAEPVIPRQGEALPNTEIFRRLAARFGFTEPCFTASDEELMDDALDAGDPRLGGARPS
                     RMPTDRATPMTAGGREFVLFENTLPKTPSGKVELASSYLDTKYGALLPDFRPVVSRFP
                     LALISPASDKRITATFGGCTGSAVAAPLEMHPDDARARRLADGARVKVWNDLGEVHLT
                     LKVTDAVPRGVVCSLKGAWMRTTDNGQTVSALAPTHKADVSEGACYNDTRVEVAAL"
     gene            complement(3574..4140)
                     /locus_tag="VLF19_01750"
     CDS             complement(3574..4140)
                     /locus_tag="VLF19_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92001.1"
                     /translation="MSERRPRMPLLVQFLPLTLFATYAFWAGPTPPPERWERAFMLGG
                     VTALVQMVLVLRQRAPVNRLLLGANLYLLVGGLATVTRQWRVLVFYGLLKEAGIFAAI
                     LLVGLVTTIFSPSGYVGRRHPDRQKVRLYSLVLLGLTAAGLVVTVLFRGSGTWPVALP
                     LLVVAIANQVLAHRLDQSPNGPAARAAR"
     gene            4164..5255
                     /locus_tag="VLF19_01755"
     CDS             4164..5255
                     /locus_tag="VLF19_01755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HSE92002.1"
                     /translation="MTSGLEDRFQTLHEIFKAAQQNLAPGPWDYLTGATETETTLRRN
                     RLAIDSIAFRPRVLRDVSRVDTSTAFLGRRMTIPVMQAPIGSIESFTPGGGATAARGC
                     AAAGVPLMLSSVCNPGLEATAAAAPDGFRIFQLYVRGDDAFVDDHVKRARDNGYAAFA
                     LTVDTAHYSRRERDLAKRFIKPWRQRATGREFQAALNWDHVKRFKDHHDVPLILKGIA
                     TAEDATVACEHGVDVVYVSNHGGRQLDHGRGSLDVLPEVVQAVAGRATIIIDGAFYRG
                     TDVIKAIALGAHVVGMGRLPCYGLAAANEAGLQRVMELLEDEIRICMGLLGVHSLGML
                     DRSYLHPGAPIVTRSSVRSAFGPLLEEGY"
     gene            complement(5252..5821)
                     /locus_tag="VLF19_01760"
     CDS             complement(5252..5821)
                     /locus_tag="VLF19_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92003.1"
                     /translation="MIALSTEYDAAQWLAAGTIMAGAVQAELGEGDTAIATIRRGLGA
                     YGSTGAHLFVPYFLSLLARACLKTGQAREGLRVIGEALEKARATGELVWEAELVRLEG
                     ELHLAASPHDVAGARDRFCRAIEIARRQGARSWELRAASSLARLHIADGRRDEARRAV
                     GGVYDSFTEGFDTADLRDAKAFLEASERG"
     gene            6534..6848
                     /locus_tag="VLF19_01765"
     CDS             6534..6848
                     /locus_tag="VLF19_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92004.1"
                     /translation="MWNQVMLDAIVAGTLGNPQAMRMAATVNTAMFDARNGVGQTYTP
                     VFETRTAPPGSHPRAAVVQAAYITLKAFYPEQLPRFDHQRARSLAEFDRDHAATVQRG
                     ID"
     gene            6909..>7667
                     /locus_tag="VLF19_01770"
     CDS             6909..>7667
                     /locus_tag="VLF19_01770"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HSE92005.1"
                     /translation="MPPFDGAGATIGQWESATGASMSPASISFTAPFVLASNGQFQWA
                     FPRPWATLDSGGYAESFNAIVTMGVKTGSGRTADQTHIAFFFNGYATNDYVEAAIQIA
                     KARQTSRRKTPRIFALLTIAMHDTSVTVFRAKRDFGMNPADVTWRPILAIPRADLDGN
                     PNTAPISGWVPRITTPNHPEYPGSHPGSHGAGPRVLQHFFGDVNASEVHPAFNGVFPG
                     PPQGGVKPRRYTRISDMAREGIAARTYGGMHFRGA"
BASE COUNT         1092 a         2773 c         2691 g         1111 t
ORIGIN      
        1 gcaagtgggc gcggcgaggc cggactgacg tcgcccgccg gcgcgtgcag cgcgctgccg
       61 cgctcgcgac tcggacgggc gccgtcaccg acgcgggacg agctggcgtc tcgcccgagt
      121 gtcgtgcctc gaacgcagcg atctcgcact cgtggccgag gatcagcgcg aactcgtcct
      181 gcccggtcag ccgcgtccgc ttccggcggg ggtcgacgtc gacgcggtgg accgcgtcgg
      241 gcggtgaacc gccgggacgc ccgcatacta tcgagaggca tggagctccc gggcggcctg
      301 cgcgcgctga accaccgcga cttccgcgtg tactacgcgg gccagctcgt ggcgctgacg
      361 gggacgtgga tgcagatcgt ggcccagtcc tggctggtcc tgcagctcac cgcctcgccg
      421 ctgcgcctcg ggctcatcgg cacgctgcag ttcgcgccgc tcctgctctt ctcgctcttc
      481 accggcgccg tcgccgaccg gctgccgaag cgccggctcc tcgtcgccac gcaatcgacg
      541 caggcgtgcc tggcgctggg tctcgcggtg ctgatcggga cgggccgcgt cgagtactgg
      601 cacgtcgcgg tcggcgcagt gctctgggga gtcgccaacg ccctggatca gcccacccgc
      661 cagtcgttcg tcgtctcgct ggtgggacgg gccgatctga tcagcgccgt cgggctcaac
      721 tcggccgcgt tcaacgccgc gcgtatcgtc gggcccgcca tcggcggcct gctgatcgca
      781 cgggccggca tcgcgctcgc cttcgcgctc aacacgctgg ccttcggcat cgccatcacg
      841 gcgctgctca cggtcggcgc gcggggcgcg gcgccgccgc gcggcggcac caccatgctg
      901 gaggagatcc gggagggcgt ggcctatgcc gtgcggtcgg cgcgcgtccg cctcgtcctc
      961 gggctcctcg cggtgacgag cttctgcgtg ttcaacttca gcgtgtacgt cccgctgctc
     1021 gcgcgcgagg tcctcggcct gggctccgag ggcttcggct tcctgatggc cgcgctcggc
     1081 gtgggcgcgg tggccgcggg actcgcgctc ggctggatcc ccagcggcgc gcctccactg
     1141 gccgccatga aggcctcgct ggccacggcg ctggccgggc tcgtggcgct ctcggccgtg
     1201 gaccggttct ggctcgcggc gctcctgctc gcggtggtcg gcttcgcggg cacgatcacc
     1261 accgcctcgt gcaacacgtc gctgcagctc acggccccgg accagctgcg cgggcgggtg
     1321 atgagcgtct acacgctgct gtcgggcggc gtcttcccgc tcggcgcctt ctgggtgggc
     1381 gcggtgtcgg aggcccgggg cgtgtccgcg gccttcctcg tcaacggcgc gctcgggctc
     1441 gccgcgctcg gcgcgctcgt cgcgtggtgg cgcacgcggc gccggcggtc cggcggctga
     1501 cggcgccggc gcgactacag agcggccacc tcgacgcggg tgtcgttgta gcaggctccc
     1561 tccgacacgt cggccttgtg ggtcggcgcc agcgccgaga ccgtctggcc gttgtcggtc
     1621 gtgcgcatcc aggcgccctt gagcgagcag accacacccc gcggcaccgc gtcggtcacc
     1681 ttgagggtca ggtgcacctc gccgaggtcg ttccagacct tcaccctcgc gccgtcggcg
     1741 agccgtcggg cgcgcgcgtc gtccgggtgc atctcgagcg gggccgcgac ggcactaccc
     1801 gtgcagccgc cgaaggtggc ggtgatgcgc ttgtcggagg ccggcgagat cagggccagc
     1861 ggaaagcgcg agacgaccgg ccggaagtcc gggagcagcg cgccgtactt cgtgtcgaga
     1921 taggacgagg cgagctccac cttccccgac ggggtcttcg gcagcgtgtt ctcgaacagc
     1981 acgaactccc ggccgccggc cgtcatcggc gtcgctcggt cggtcggcat ccgactcggt
     2041 cgcgcgccgc ccagacgcgg atcgcccgcg tcgagggcgt cgtccatcag ctcctcgtca
     2101 ctcgccgtga agcacggctc cgtgaacccg aagcgcgccg ccagccgccg gaagatctcg
     2161 gtgttgggga gcgcctcgcc ctggcgcggg atgaccggct cggcgcgctg gagccagtgc
     2221 tggccgtaag ccgcgaagag atcggcgtgc tcgaagtgac tgcaggccgg cagcacgaca
     2281 tcggcgtagg ccaggctgtc cgtcatcacc acgtcgcagc cgacgacgaa caggtcctca
     2341 cgcgccaggc cgcgccgcag ggtgttctgg tcggggtgca cgaccagcgg gttgtgattg
     2401 tagatgaaga ccgctccgac cggcggcttc aggcgcgcgt cgagcaggtg cgcgcccacg
     2461 tcgatgatgt tgagcgtccg cgtccccggc gggatcaggt ccggccgcgc caggcgctgt
     2521 ggcgtcgtcg ggaaggccag gcgggcgccg ttgacgagcc cgccgccgcg cacgccccac
     2581 ttcccggcga gcgcgggtag cgcgaagacc gcgcggatac cgctgccgcc gttctggttg
     2641 cgctcgaggc cgttcccgac gctgatcgcc gcgggcgaga gcgtggcgta ccactcggcg
     2701 agcgtgcgga tctgggcctc gggcacgcca cacagcggcg cggccgtggc gacgggccag
     2761 ggccgcacgc gctccatgta accatcgaag ccgtgcacgt gacgcgcgat gaactcgcgg
     2821 tcgagcgcgc cgaggcgctc gagttccccg gtgaccgcga acgccagcag cacgtccgtc
     2881 cccggccgaa gcgcgagatg cagatccgcc tgctcggcga tcttcgtgcg cctgggatcg
     2941 acgaccacca gcctggcgcc cttgcgcctc gcgcggtgga tgatcggcgt caggtgcaga
     3001 ttgcaccagg tgacgttgtt gccccagacg acgatcagcc gcgcgtcctc gacctgctcg
     3061 ggccggatgc ccggtacgct cccgtaggtg ccgatccacg actccgtgcg gatgccgccg
     3121 cagagcggct tgcggtccag gaggctcgcg ccgagccggt ggaagaagcg caggtccatc
     3181 gatccgccgg cgaggaagcc gtgcgggccg gcgtagttga gcggcaggat cgcctgccgc
     3241 ccgtgctcgg ccatgacggc agtgaaccgg tcgtggatct gatcgagcgc ctcgtcccac
     3301 gagatgcgcg cgaaccggcc cccgcccttc gcgcccacgc ggcgcagcgg cgtgcgcagc
     3361 cgcccgggcc cgtgcacgaa ctcgggatac gacgccggca ccttcgcgca cagcacgccc
     3421 tccgtgtacg gattggcgcg cgtgccgcgg atgtcgacga tcgtgtcgtt ctcgacggtg
     3481 accgcgaggc tgcaggtgtc ggggcaatcg agcgggcaga cgctggcctt cgtgactcgc
     3541 gcggtgaggg tgcccatggg tgaggcgatt ctatcagcgc gcggcgcgcg ccgccggccc
     3601 gttcggcgat tggtcgagcc ggtgcgccag cacttggttg gcgatggcga ccacgagaag
     3661 aggcagcgcc accggccacg tgcccgagcc ccggaacaga accgtgacga cgagcccggc
     3721 cgcggtcaat ccgagcaaga cgagcgagta gagccgcacc ttctggcggt cggggtgccg
     3781 ccgtcccacg tagcccgacg ggctgaagat cgtcgtcacg agccccacca ggaggatcgc
     3841 ggcgaagatg cccgcctcct tgaggaggcc gtagaagacc agcacgcgcc actggcgggt
     3901 gaccgtcgcc agtccgccga ccagcaggta gagattcgcg ccgagcagga ggcggttcac
     3961 gggagcgcgc tggcgcagga cgaggaccat ctgcacgagg gccgtcacgc cgccgagcat
     4021 gaacgcccgc tcccagcgct ccggcggcgg cgtcggtccc gcccagaacg cataggtggc
     4081 gaagagcgtg agaggcagga actggacgag cagcggcatg cgcgggcggc gctcggacac
     4141 gccgtcatgc tataacaccg gccatgactt caggactcga ggaccgcttc cagaccctgc
     4201 acgagatctt caaggccgcc cagcagaacc tggcccccgg gccgtgggac tacctgacgg
     4261 gcgccaccga gaccgagacg acgctccggc gcaaccgcct ggcgatcgac tcgatcgcgt
     4321 tccggccgcg ggtcctgcgc gacgtctcac gcgtggacac gtcgacggcc ttcctgggcc
     4381 gacgcatgac catccccgtc atgcaggccc cgatcggctc gatcgagtcg ttcacgccgg
     4441 ggggcggggc gacggcggcc agggggtgcg cggcggccgg cgtgccgctg atgctctcgt
     4501 cggtgtgcaa ccccgggctc gaggccacgg ccgcggcggc gcccgacggc ttccggatct
     4561 tccagctcta cgtgcgcggc gacgacgcct tcgtggacga ccatgttaag cgcgctcgcg
     4621 acaacggcta cgccgccttc gccctcacgg tggacaccgc acactacagt cggcgcgagc
     4681 gcgacctggc caagcgcttc atcaagccct ggcggcagcg cgccaccggc cgggagttcc
     4741 aggccgcgct gaactgggat cacgtcaagc gcttcaagga ccaccacgac gtgccgctga
     4801 tcctcaaggg catcgccacg gccgaggatg ccacggtggc ctgcgagcac ggtgtggatg
     4861 tcgtctacgt ctccaaccac ggcggccgcc agctcgacca cggccgcggc tcgctcgacg
     4921 tgctccccga ggtcgtccag gccgtcgccg gccgcgccac gatcatcatc gacggcgcct
     4981 tctaccgcgg caccgacgtg atcaaggcga tcgcgctggg cgcccacgtg gtcggcatgg
     5041 ggcgcctgcc ctgctacggg ctggccgcgg ccaacgaggc cggactgcag cgcgtgatgg
     5101 agctgctgga ggacgagatc cggatctgca tgggcctgct cggcgtgcac tcgctcggaa
     5161 tgctcgacag gtcgtacctg caccccggcg cgccgatcgt cacccgctcc agcgtccgga
     5221 gcgccttcgg gccgctgctg gaggagggat actaaccccg ttcactcgcc tccaggaagg
     5281 ccttcgcgtc ccgaaggtcg gcggtgtcga agccttcggt gaacgaatcg tagacgccgc
     5341 cgacggcgcg ccgcgcctcg tcgcgccgac cgtccgcgat atgcaggcgc gcgaggctcg
     5401 aggcggcgcg cagctcccac gaccgtgcgc cctgccggcg cgcgatctcg atcgcccggc
     5461 agaagcggtc ccgggctccg gcgacgtcgt gcggagatgc ggcgaggtgt agctctccct
     5521 cgagccgaac cagctccgcc tcccacacga gctcacccgt cgcgcgggcc ttctcgaggg
     5581 cctcgccgat cacacgcagc ccctctcgag cctggccggt cttcaggcag gcgcgggcca
     5641 gcagggagag gaagtacggc acgaaaaggt gcgcgccggt cgacccgtag gccccgagcc
     5701 cgcgcctgat cgtcgcgatc gccgtgtcgc cctcgccgag ctcggcctgg acggcccctg
     5761 ccatgatcgt gcccgccgcc agccactgcg ccgcgtcgta ctcggtcgag agggcgatca
     5821 aggcacgggc gtgcttgccg acctcgtgcg gttcgacgca ggtcgcgaaa gacgcacgcc
     5881 cacacgagcg cgacgatgat gctcggggga tgatcgagct ccctggccag cctgagcgac
     5941 atgacgctct cgtcgaggcc gcgcgccgga tggccgagga tccaggaggc ccagctcgag
     6001 accttccagc agcacacgcc ggggtcgtgg ccaccataga ggaacgcgtg gctccggtgc
     6061 cctgtcttgt cgtacagcgt cttgccctga tccaggtgcc ggagtgcggc gttcgcctct
     6121 cccagccaca cgagcaccgg ccagagcgcg tggtgcccct cgagcaggag cgccgggtcg
     6181 tcgaggcgct cggcgagtgc caggagctct cgtccgagct cgagcgcggc atggcgcgag
     6241 atgcgtcgcc cacgatagca cggcccccac ccgctggcca cgcggccctt gacgcggttt
     6301 ttgacgccgg cctctacgaa tatcgaacgc ccgtccatta cacagggatg cgtggagcgg
     6361 atcctctgcg gctccgacca caggacacat ggtgacatcg tggcgaccaa gagggggcca
     6421 tgcttcgatt tcatcggctc atgcttctct cgttcgtggg agccctgctt ctctcaccgc
     6481 ccgggctcgg aacggcctcg ggggaggcgg tcaggaaggt cgacgagacc ctggtgtgga
     6541 accaggtcat gctcgatgcg atcgtcgcgg ggactctcgg caatccgcag gccatgcgca
     6601 tggcggcgac cgtgaacacc gcgatgttcg acgcccgaaa cggcgtcggc cagacctaca
     6661 cgcccgtctt cgagactcgg accgcgccgc cgggaagcca tcctcgcgca gcggtggtgc
     6721 aggctgccta catcaccttg aaggcatttt acccggagca gctgccccgg ttcgaccacc
     6781 agcgggcccg atccctcgcc gaattcgatc gcgaccacgc ggcgaccgtc cagcgcggca
     6841 tcgactgagg cgagagcgtc gcgaagcacg tcctggcgtg gcgcgggacc gacggcttca
     6901 gcaatccggt gcctccgttc gacggcgcgg gcgcgacgat cgggcaatgg gagtccgcga
     6961 ccggcgccag catgtcgccg gccagcatct ctttcaccgc tccgttcgtg ctggccagca
     7021 acggccagtt ccagtgggcc tttccgcggc catgggcgac cctcgacagc gggggctacg
     7081 ccgagagctt caacgcgatc gtgacgatgg gcgtgaagac cggctctggc cgcaccgcgg
     7141 accagacgca cattgcgttc ttcttcaacg gctacgccac caacgactac gtcgaggcgg
     7201 ccatccagat cgccaaggct cgccagacat cgcgccgtaa gacccctcgc atcttcgcgc
     7261 tgctcaccat cgcgatgcac gacacgtcgg tgacggtgtt ccgcgccaag cgagacttcg
     7321 gcatgaatcc cgcggacgtc acctggcggc ccatcctcgc cattcccagg gcggatctcg
     7381 acggcaaccc caacaccgcc ccgatctccg gctgggttcc gcgcatcacg acaccgaatc
     7441 acccggagta cccgggctcg catccgggat cgcacggcgc ggggccccgc gtgctccagc
     7501 acttcttcgg cgatgtgaac gcctccgagg ttcatccggc gttcaacggc gtcttcccgg
     7561 gccccccgca ggggggagtg aagccgcggc gctacacgcg catctcggac atggcgcgag
     7621 aaggcatcgc cgcccgcacc tacggcggca tgcacttccg cggcgcg
//
LOCUS       DATIAZ010000053        12056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_406961, whole genome shotgun sequence.
ACCESSION   DATIAZ010000053 DATIAZ010000000
VERSION     DATIAZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406961"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..690
                     /locus_tag="VLF19_01775"
     CDS             37..690
                     /locus_tag="VLF19_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE92006.1"
                     /translation="MDPQQEIEPYLEAAGQKGMRITHVFETHVQADHVSGARALAAVT
                     GAPVLYHESAEVRFPHVELADGEEHELGNVRLSVLHTPGHTPDSVCLMVTDRSRGPEP
                     WFVLTGDTLFSGGVGRPDLLGQGAEERLAGQLYESLHGRLLALPDHLEVHPAHFGGAA
                     CGKGLSGKPGSTIGFERRFNPALRRPGKDDFVRFVLADLPPQPPAFAENRRRNLGLE"
     gene            788..1927
                     /locus_tag="VLF19_01780"
     CDS             788..1927
                     /locus_tag="VLF19_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92007.1"
                     /translation="MLPLLGEQEFGLASKTAITSFIVSFGVTKAVVNLVAARVSDRVG
                     RKPILVAGWLFALPVPFLIIHAPAWWWIDLANVLLGANQAMAWSMTVIMKIDLVGPKR
                     RGLALGLNEFAGYFAVGLMSWVTGYIAAQTALRPQPFYLGIVIAAVGLALSVLFVRET
                     RPYAHLEAATAPPAPSGGRSSLGSIFYLTSLGNASLFAACQAGLVNNLNDGMSWGLYP
                     LFFAAHGLGVASIGVIKAVYPLVWGVLQVVTGPLSDRFGRKELIAGGMIVQAAGIWLT
                     VLVPAYAAWLAGAALQGLGTAMVYPTLLAAITDHAHPTWRATSLGVYRFWRDLGYAVG
                     ALLSGAVADLVGLGAAIHLVAALTLASGFVVMGAMRKRRSVAVPS"
     gene            complement(2046..2933)
                     /locus_tag="VLF19_01785"
     CDS             complement(2046..2933)
                     /locus_tag="VLF19_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE92008.1"
                     /translation="MAVPPITITATKVTPRLYFVEGLPGVASVANRGFNSNAGFVVTD
                     EGVVVVDALGTEPLGAALVDAIRKVTTRPIRRVIVTHYHADHFYGLGPLKAAGAEVWA
                     HHLGREYLETQGGARLAQRRQDLAPWVDERTRLVPADRWLAGDTTFAMGGVDFDVVHM
                     GPAHAPDDVIVVVRQEQAIFCGDIMISGRVPFVGEADSKRWLVTLDRLLALRPRLMVP
                     GHGGVSRDPARDLAFTRDYLLYLRKTMGRAVADLVPFDEAYAATDWSRYAAVPAFDAA
                     NRINAYGTYLLMERESLGR"
     gene            complement(2944..4656)
                     /gene="soxB"
                     /locus_tag="VLF19_01790"
     CDS             complement(2944..4656)
                     /gene="soxB"
                     /locus_tag="VLF19_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HSE92009.1"
                     /translation="MTRREFLRTLALAAAAGLPLDARPAAAQADRFYDVPPFGDVSLL
                     HFTDSHGQLLPLYYREPSVNLGVGAGRGQPPHLVGEKLLAHFGVARGSREAHALTHLD
                     YVQAAATYGKVGGFAHLRTLVKRLRATRPGAILLDGGDTWQGSATALWTRGQDMVDAA
                     KLLGVEVMAGHWEFTLGAERVKEIVGKDFKGRIDFLAQNVRTTDFEDAVFPAYVVREV
                     RGVPIAVIGQAFPYTPIANPRYFTPEWSFGIREDNLQAVVDEARGKGAQAVVLLSHNG
                     MDVDLKLAGRVRGIDAILGGHTHDGVPAPTIVRNAGGRTLVTNAGSNGKFLAVLDLAI
                     RDRKVADFRYRLLPVFSNLLPGDQEMAAHIRAVRAPYETRLAEPLAVTRALLYRRGNF
                     NGPFDQVILDALMAVKGAQIAFSPGFRWGVSLLPGETITFEHVMGQTAITYPYTTVTE
                     MTGETIKAILEDVGDNLFNPDPYYQQGGDMVRVGGLTYACDPAAKVGARISDLRLGGV
                     PLDAAKRYKVAGWAPVSEEARAAGGEPIWDVVARYLRDRKTIVPPVVNVPRLIGVAGD
                     PGLA"
     gene            complement(4673..5290)
                     /gene="soxX"
                     /locus_tag="VLF19_01795"
     CDS             complement(4673..5290)
                     /gene="soxX"
                     /locus_tag="VLF19_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HSE92010.1"
                     /translation="MKAIRIAASAALVLLLGASGSGDAQSDRKAVDVMKSSFKARGQA
                     QLDRLDQDEMQAACSRLPRDGALPGVVAERIQKANLEAVKYPADGKFMGDWKQGERIA
                     QTGVGMQFSDDPKRPAGGNCYACHQLSGAELSYGTIGPSLRNYGKVRGASLEVQRFTY
                     TKIYNSHAYQACSNMPRFGHKGILTEEQLKHLVALLLDPDSPVNK"
     gene            complement(5299..6075)
                     /gene="soxA"
                     /locus_tag="VLF19_01800"
     CDS             complement(5299..6075)
                     /gene="soxA"
                     /locus_tag="VLF19_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HSE92011.1"
                     /translation="MALGGYVASAQQGEDTVKGIEKYREALADANPAELWEARGEALW
                     KEKRGPMTVSLERCDLGKGPGVVAGAYAVLPRYFADADRVMDLETRLVHCMVTLQGFT
                     PAEATRQKYGSGPKRSDLEALVAFVTAESRGHVMNVPLGHPKEKEAYALGERIFFFRG
                     GSHDFSCASCHSENGKRIRLQDLPNLTEQKDARRAYTTWPAYRVSQGELRTMQWRLYD
                     CFRQQRFPELEYGSEASIALTMFLARNANGGTFNAPAIKR"
     gene            complement(6149..6577)
                     /locus_tag="VLF19_01805"
     CDS             complement(6149..6577)
                     /locus_tag="VLF19_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSE92012.1"
                     /translation="MSAYRLAPALLFLASMVVTPARAEDPIKVVYHLNEGIPQAARAL
                     GNVRNHLAADPTAKIVVVTHGNGIEFLLKDAKKPSGAAFSNDIEELSLRGVEFRVCQN
                     TLETRKIDPARVVEEGKIVRSGVAEVARLQAREGYVYVRP"
     gene            complement(6574..6885)
                     /gene="soxZ"
                     /locus_tag="VLF19_01810"
     CDS             complement(6574..6885)
                     /gene="soxZ"
                     /locus_tag="VLF19_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HSE92013.1"
                     /translation="MADPMRIRASIQGDTVQVRVLMSHEMETGQRKDSAGNVVPAHFI
                     QSVVATHNGRQVFRAEWGPAVSKNPYLQFQFKGAKTGDKVMITWSDNKGDSRTDEGVI
                     R"
     gene            complement(6958..7446)
                     /gene="soxY"
                     /locus_tag="VLF19_01815"
     CDS             complement(6958..7446)
                     /gene="soxY"
                     /locus_tag="VLF19_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HSE92014.1"
                     /translation="METWKVGRRQALKVLGAAAVAFGGPALLVRGAVAQQQAMAWNKD
                     AFAAQSVADVLKALGAGSPAATQDVSWGATPAIAENGAVVPVNVTSKIPDTESIAIII
                     EKNPNKLAGNFFFPEGTEPALATRVKMSESSNVHAVVRTKSGKAFMATREIKVTLGGC
                     GG"
     gene            complement(7464..8531)
                     /locus_tag="VLF19_01820"
     CDS             complement(7464..8531)
                     /locus_tag="VLF19_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSE92015.1"
                     /translation="MFRSARGVAALAGLLLVAGAAHAQGRFPGVGRPATPAEVQVWDI
                     DVRPDFTGLPRGSGSVARGQEAWDDKCASCHGTFGESTQVFPPIVGGTTKADVASGRA
                     AALRSPSEQRTTLMKLSTLSTLWDYINRAMPWHAPKTLTVEEVYALVAYILHLGEIVP
                     ADFVLSDANIREVQRLLPNRAGMTRDHGLWDVAGRPDVTGTACMRNCSAEPKVTSAIP
                     ERARDAHGNLMTQHRVVGPVRGADTTRPPLASAEETKDLAQRALAALSVTDGAAGGFD
                     LAKRLACVACHLVEQRLVGPSFKEIAGKYRGTADGRAAVATKIRAGGVGAWGPVPMPP
                     QPQVSEAEAETLARWIIAEAQ"
     gene            complement(8515..9846)
                     /gene="soxC"
                     /locus_tag="VLF19_01825"
     CDS             complement(8515..9846)
                     /gene="soxC"
                     /locus_tag="VLF19_01825"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HSE92016.1"
                     /translation="MARRLIPRGRILPAPESFLGPRQIAAIDSGRRGFLASGLAAVAA
                     VAAARAASAADAGDPAILTLPPHSASLGQPVAARRYGVPSKWEVNLQRRQSPGLTRVD
                     AASVSFAPLQGFFGIITPNGLHFERHHQGWHDIDPSRHRLMVNGLVKAARVYTMDDLM
                     RLPAVSRIHFIECGANSGMEWGNVAVPTVQYTHGMLSCCEFTGVPLATLLDDCGADLK
                     KGKFVLAEGADGSGLTRTIAMERALDDVIVAWGMNGEMLRPENGYPLRLVVPGVQGVS
                     WVKWLRRLEVGDQPYGTKDEVLHYVDLMPDGRHRQYTSIQECKSVITTPSGGQVLLAR
                     GYYNVTGLAWSGRGRVKRVDVSVDGGRSWRIARLEAPVLPKSLTRFSLDWVWDGRDAI
                     LQSRAADETGYVQPAIAQLRAVRGTRSIYHNNAIQSWKVTAAGDVSNVQVG"
     gene            complement(9831..10172)
                     /locus_tag="VLF19_01830"
     CDS             complement(9831..10172)
                     /locus_tag="VLF19_01830"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSE92017.1"
                     /translation="MVRALGHPTRLRILDLVEGREVAYPELLRETGVSKANLSQHLGV
                     LRAGHLLTERRHGREVHFRLTYPGIKVLCGAMRQILARHLDRQARVADAGRREVARAG
                     VRRRRQQWRAG"
     gene            complement(10331..10987)
                     /locus_tag="VLF19_01835"
     CDS             complement(10331..10987)
                     /locus_tag="VLF19_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92018.1"
                     /translation="MWRAAVAIVLGVALAGAGLALTVSAQQAPASGPRQPHDHGSMPS
                     HDPDTHAQHMQAMHGRAGGGAPALPGQDAFGAIAEVVAILEADPNTDWSKVDLERLRQ
                     HLIDMSEVVLRSTVRQTPVPAGLSMDITGTGRTEQAIRAMVVPHAVELDRMPSLAART
                     ETIAGGVRLTVTTRRADDAKLVARIRGLGFAGLITAGAHHQPHHLAMARGEALDGHRH
                     "
     gene            complement(11051..11782)
                     /locus_tag="VLF19_01840"
     CDS             complement(11051..11782)
                     /locus_tag="VLF19_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSE92019.1"
                     /translation="MTPPRASVAATVEVLRRVSYFAALPATEVRRLAGQCTARRLGPG
                     ETLFEEGEPCRGLLVVAEGRVEIRQISLRGREQVFHTEGPGATLGEGPLFDGGGYIAS
                     AVALAPTQVLFLPRAEVLGLCRRRPEVALAMLKTMARRVRHFAGIVGDLAFRPVTERL
                     ARYVATAVVGPVEPGASVELILTHAQLAARLGTVRELIARAFSQLEQGGVISRERSRI
                     VIRDPARLAALARGDEDAARGGGVT"
     gene            complement(11793..>12056)
                     /locus_tag="VLF19_01845"
     CDS             complement(11793..>12056)
                     /locus_tag="VLF19_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92020.1"
                     /translation="TQTQLISFFVNPPLAMLSGGLTPIEAMPGWVQPLTALNPIAHFA
                     TIARSVLVKGAGLDVIYPNLLALATLASLLVGVSAWRFRRQLS"
BASE COUNT         1631 a         4356 c         4212 g         1857 t
ORIGIN      
        1 ctcctcggct gagggggcgc aggcctctgc gctgtggtgg atccacagca ggagatcgag
       61 ccctacctcg aggcggccgg gcagaagggg atgcgcatca cccacgtttt cgagacccac
      121 gtccaggccg accacgtctc cggggcgcga gcgctggccg ccgtcacggg ggccccggtc
      181 ctctatcacg agtccgccga ggtgcgcttc ccgcacgtcg agctggcgga cggcgaagag
      241 cacgagctgg gcaacgtgcg gctctcggtg ctccacacgc ccggccacac gccggacagc
      301 gtctgcctga tggtcaccga tcggagccgg ggacccgagc cgtggttcgt cctcaccggc
      361 gacacgctct tctcgggcgg cgtcggccgg ccggacctcc tgggccaggg cgccgaggag
      421 cggctcgccg ggcagctcta cgagagtctc cacggccggc tgctcgcgct gccggatcac
      481 ctcgaagttc accccgcgca cttcggcggc gccgcctgcg gcaagggcct cagcggcaag
      541 cccggctcga cgatcggctt cgagcgccgg ttcaacccgg ccctccggcg gccgggcaag
      601 gacgacttcg tgcgcttcgt gctggccgac ctgccgcccc agccgccggc cttcgccgag
      661 aaccgccgcc ggaacctggg gctcgagtag cgcgatccgg ctcgggctgc gcgagaacct
      721 cggccagttc tcgctgctcg tcgtcctcaa cgccttcgtc ggcgcgatgg tggggctgga
      781 gcgcacggtg ctcccgctcc tcggcgagca ggagttcggg ctcgcgtcga agacggccat
      841 cacctcgttc atcgtgagct tcggcgtgac caaggccgtc gtcaacctcg tggccgcgcg
      901 cgtgtccgac cgcgtcgggc gcaagccgat cctggtggcc ggatggctct tcgccctgcc
      961 cgtgcccttc ctcatcatcc acgccccggc gtggtggtgg atcgacctcg ccaacgtgtt
     1021 gctgggcgcg aaccaggcca tggcgtggtc gatgacggtc atcatgaaga tcgatctcgt
     1081 ggggccgaag cggcgtggcc tggctctggg actgaacgaa tttgccggct acttcgcggt
     1141 gggactgatg tcgtgggtca ccgggtacat cgccgcacag acggcgctgc ggccgcagcc
     1201 gttctatctc ggcatcgtca tcgccgccgt cggtctcgcg ctctccgtcc tgttcgtgcg
     1261 agagacgcgc ccctacgccc acctcgaggc cgcgacggca ccgccggcgc cgagtggcgg
     1321 ccgctcatcg ctcggcagca tcttctacct gacgagcctc ggcaacgcga gcctcttcgc
     1381 cgcctgccag gccgggctcg tgaacaacct caacgacggc atgtcgtggg gtctctaccc
     1441 gctgttcttc gccgcccacg gcctcggcgt cgccagcatc ggcgtgatca aggccgtgta
     1501 cccgctcgtc tggggcgtcc tccaggtcgt gaccggtccc ctctccgacc gtttcggccg
     1561 caaggagctc attgccggag ggatgatcgt gcaggccgcc gggatctggc tgaccgtgct
     1621 cgtgcccgcc tacgccgcgt ggctggccgg ggcggcgctc cagggactcg gcacggccat
     1681 ggtctatccg acgctgctcg ccgccatcac ggaccacgcc catccgacgt ggcgcgcgac
     1741 gagcctcggc gtctaccgct tctggcgcga cctggggtac gcggtcggcg cgctcctgtc
     1801 cggcgccgtc gccgacctcg tcggactcgg ggccgcgatc cacctcgtgg cggcgctcac
     1861 gctcgcctcc ggcttcgtcg tgatgggcgc gatgcggaaa cgccgctccg ttgccgtgcc
     1921 atcatgagta catgccgacc tctcgctctg ggggtcactg catgaccgag cccccggtcc
     1981 gcggcgccgt tgtgagcggg taggcgattc ttggggcagg cccggggtcc gtggcccgcg
     2041 gggccctacc ggccgagcga ctcccgctcc atcaagaggt atgtgccgta ggcgttgatc
     2101 cggttcgccg cgtcgaaggc cggcaccgcc gcgtagcggc tccagtccgt cgccgcgtac
     2161 gcctcgtcga agggcaccag gtcggccacc gcgcgcccca tcgtcttccg gaggtacagc
     2221 aagtagtcac gcgtgaaggc gagatcgcgg gcgggatctc gcgagacgcc gccgtgcccc
     2281 ggcaccatca ggcgcggccg gagcgcgagc agacggtcga gcgtcaccag ccaccgcttg
     2341 ctgtcggcct ccccgacgaa cggcacgcgc ccgctgatca tgatgtcgcc gcagaagatc
     2401 gcctgctcct ggcggacgac gacgatgacg tcgtcgggcg cgtgcgccgg ccccatgtgg
     2461 acgacgtcga agtcgacgcc gcccatcgcg aacgtcgtgt cgccggccag ccaccggtcg
     2521 gcgggcacca gccgcgtccg ctcgtcgacc cacggcgcca ggtcttgccg gcgctgggcg
     2581 agccgcgcgc cgccctgggt ctcgaggtat tcgcggccga ggtggtgggc ccagacctcg
     2641 gcgccggcgg ccttgagcgg gcccaggccg tagaagtggt cggcgtggta atgcgtcacg
     2701 atcacgcggc ggatgggccg cgtcgtcacc ttgcggatgg cgtcgaccag ggcggcgccg
     2761 agcggctcgg tcccgagggc gtcgaccacg acgacgccct cgtcggtgac gacgaatccg
     2821 gcgttcgagt tgaagccacg gttggccacc gaggccacgc ctggcaggcc ctccacgaag
     2881 tagaggcgcg gcgtgacctt cgtcgcggtg atcgtgatcg gcgggacggc catgccgccc
     2941 acctcaggcg agcccggggt cgccggcgac gccgatcagc cgcggcacgt tcacgacggg
     3001 gggcacgatg gtcttgcggt cgcgcaggta gcgcgccacc acgtcccaga tcggctcgcc
     3061 ccccgccgcg cgagcctcct cggagacggg cgcccagccg gcgaccttgt aacgcttcgc
     3121 cgcgtcgagg ggcacgccgc ccaggcgcag gtcgctgatc cgcgcgccaa ccttcgccgc
     3181 cggatcacag gcgtaggtga ggccgccgac gcgcaccatg tcgccgccct gctggtagta
     3241 gggatccggg ttgaagagat tgtcgccgac gtcctcgagg atcgccttga tcgtctcccc
     3301 ggtcatctcc gtcaccgtcg tgtacgggta cgtgatggcc gtctggccca tcacgtgctc
     3361 gaaggtgatc gtctcgccgg gcagcagcga cacgccccag cggaacccgg gggagaaggc
     3421 gatctgggcg cccttcaccg ccatgagggc gtcgaggatg acctggtcga acgggccgtt
     3481 gaagttgccg cgccggtaga gcagcgcccg ggtcacggcg agcggctccg cgagcctcgt
     3541 ctcgtagggt gcccgtactg cgcggatgtg ggccgccatc tcctgatcgc cgggcaggag
     3601 gttcgagaag acgggcagga ggcggtagcg gaagtcggcg accttcctgt cgcggatcgc
     3661 gaggtcgagc accgccagga acttgccgtt ggagccggcg ttcgtcacca gtgtgcggcc
     3721 gccggcgttc cgcacgatcg tcggcgccgg cacgccgtcg tgagtgtgcc cgccgaggat
     3781 cgcgtcgatg ccgcgcaccc gaccggcgag cttcaggtcg acgtccatcc cgttgtgcga
     3841 gaggaggacg acggcctgcg cgcccttgcc acgggcctcg tccaccaccg cctggaggtt
     3901 gtcctcgcgg atcccgaagc tccactccgg cgtgaagtag cgggggttgg cgatgggcgt
     3961 gtacgggaat gcctgtccga tgacggcgat gggcacgccg cgcacctcgc gcacgacgta
     4021 ggcgggaaac accgcgtcct cgaagtccgt ggtcctgacg ttctgcgcga ggaagtcgat
     4081 ccggcccttg aagtccttcc cgacgatctc cttcactcgc tcggcgccca gcgtgaactc
     4141 ccagtgcccc gccatcacct ccacgcccag cagcttcgcg gcgtcgacca tgtcctggcc
     4201 gcgcgtccac agcgccgtcg ccgacccctg ccacgtgtcg ccaccgtcga gcaggatcgc
     4261 gcccggccgg gtcgcgcgca gccgcttcac cagcgtgcgc aggtgcgcga agccgccgac
     4321 cttgccgtac gtcgccgcgg cctggacgta gtcgaggtgc gtgagggcgt gcgcctcgcg
     4381 cgagccccgc gcgacgccga agtgtgcgag cagcttctcg ccgacgaggt gcggcggctg
     4441 cccgcggccc gcgcccacgc cgaggttgac gctcggctcg cggtaataga ggggcaggag
     4501 ctggccgtgc gagtcggtga agtgcaggag gctgacgtcg ccgaacgggg ggacgtcgta
     4561 gaagcggtcg gcctgcgcgg ccgccggccg cgcatcgagc ggcaggccgg cagctgcggc
     4621 gagcgccagc gtacggagga actcgcggcg ggtcaccggc cgcccccccg ttctacttgt
     4681 tcaccggcga gtccgggtcc agcagcagcg ccaccaggtg cttgagctgc tcctcggtca
     4741 ggatgccctt gtggccaaag cgcggcatgt tcgagcacgc ctggtaggcg tgcgagttgt
     4801 agatcttggt gtaggtgaag cgctgcacct cgagcgaggc gccccgcacc ttcccgtagt
     4861 tgcgcaggct gggcccgatg gtcccgtagg agagctcggc cccggagagc tggtgacagg
     4921 catagcagtt gccgccggcg ggtcgtttcg ggtcgtcgct gaactgcatg ccgacgccgg
     4981 tctgggcgat ccgctcgccc tgcttccagt cgcccatgaa cttgccgtcg gccgggtact
     5041 tcacggcctc gaggttggct ttctggatgc gctcggccac gaccccgggc agggcgccgt
     5101 cccggggcag ccggctgcac gccgcctgca tctcgtcctg gtcgagccgg tcgagttggg
     5161 cctggccgcg tgccttgaac gagctcttca tcacgtccac ggccttcctg tccgactgcg
     5221 cgtccccgga ccccgaggcg ccgagaagga ggacgagcgc cgcgctcgcc gcgatcctga
     5281 tcgccttcat gtcgtcccct atcgcttgat ggcgggcgcg ttgaacgtgc cgccgttggc
     5341 gttacgagcc aggaacatcg tcagcgcgat cgaggcctcg gagccgtact ccagctccgg
     5401 gaaccgctgc tgccggaagc agtcgtagag gcgccactgc atcgtccgca gctccccctg
     5461 cgagacgcga taggccggcc acgtcgtgta ggcgcgccgg gcgtccttct gctcggtgag
     5521 gttcggcagg tcctggagcc tgatgcgctt gccgttctcg ctgtggcacg aggcgcagga
     5581 gaaatcgtgc gagcccccgc ggaagaagaa gatgcgctcg ccgagcgcgt acgcttcctt
     5641 ctccttggga tggcccaggg gcacgttcat cacgtggcct ctggactccg cggtcacgaa
     5701 ggcgacgagg gcctcgaggt ccgaccgctt cggcccgctc ccgtattttt gcctggtcgc
     5761 ctccgccggc gtgaagccct ggagggtcac catgcagtgc acgagccgcg tctccaggtc
     5821 catcacccgg tcggcgtccg cgaagtagcg cggaaggacg gcgtaggcgc cggcgaccac
     5881 gccgggcccc ttgccgaggt cgcaccgctc gagggagacg gtcatcgggc cgcgcttctc
     5941 cttccacagc gcctcccccc gcgcctccca cagctcggcc gggttggcgt cagcgagcgc
     6001 ttcccggtat ttctcgatgc ccttcaccgt gtcctcgcct tgctgggcgc tggcgacgta
     6061 cccgcccagc gccatcccgc ccacgaggac cgccgcgacg agcatccacc accgcgcgta
     6121 tcgcatcatg gcctcgcctc cagacccctc agggccggac gtagacatag ccctctcgcg
     6181 cctgcagccg ggccacctcg gccacgcccg accgcacgat cttgccctcc tcgaccacgc
     6241 gggccgggtc gatcttccgg gtctcgagcg tgttctggca gacgcggaac tccacgccgc
     6301 ggagcgacag ctcctcgatg tcgttgctga aggccgcgcc cgagggcttc ttcgcgtcct
     6361 tcagcaggaa ctcgatgccg ttgccgtgag tgaccacgac gatcttcgcg gtcgggtcgg
     6421 cggccaggtg gttccggaca tttccgagcg cccgcgcggc ctgcgggatg ccctcgttca
     6481 gatgatagac gaccttgatc gggtcctcgg cgcgggcggg cgtgaccacc atcgaggcga
     6541 ggaagagcag cgcgggcgcg agccgatagg cgctcatcgg atcaccccct cgtcggtccg
     6601 gctgtcgccc ttgttgtcgc tccacgtgat catgaccttg tcgccggtct tggcgccctt
     6661 gaactggaac tgcaggtagg ggttcttcga caccgcgggc ccccactcgg cccggaacac
     6721 ctggcggccg ttgtgcgtgg ccaccacgga ctggatgaag tgggccggga cgacgttccc
     6781 ggcagagtcc ttccgctggc ccgtctccat ctcgtggctc atgagcacgc gcacctgcac
     6841 ggtgtcgccc tggatcgacg cgcgaatccg catggggtca gccatttgcc gcctctcctg
     6901 ctgtttgctg tcggttcgtg tggaaacgtg gctcgtccgg tcgagtcgtc gggcggccta
     6961 cccgccgcag cccccgaggg tgaccttgat ctcgcgcgtc gccatgaacg ccttgccgct
     7021 cttcgtccgg acgaccgcgt gcacgttgga ggactcgctc atcttgacgc gggtcgccag
     7081 cgccggctcc gtgccctccg ggaagaagaa gttcccggcg agcttgttcg ggttcttctc
     7141 gatgatgatg gcgatcgact ccgtgtccgg gatcttgctg gtgacgttca ccggcaccac
     7201 cgcgccgttc tcggcgatgg ccggcgtcgc gccccacgac acgtcctgcg tcgccgccgg
     7261 gcttcccgcg ccgagcgcct tcaggacgtc ggccacgctc tgggcggcga acgcgtcctt
     7321 gttccacgcc atcgcctgct gctgcgccac tgcgccacgc acgagcagcg ccgggccgcc
     7381 gaacgcgacc gccgccgccc ccagcacctt cagcgcctgt ctccgaccga ccttccacgt
     7441 ctccatcgtc gtgccttcct ccctcactgt gcctctgcga tgatccaccg ggccagcgtc
     7501 tcggcctcgg cctcgctcac ctggggctgc ggcggcatcg gcaccggccc ccacgcaccg
     7561 accccgcccg cccggatctt cgtggcgacg gcggccctgc cgtcggccgt gccgcgatac
     7621 ttgccggcaa tctccttgaa cgacgggccc accagccgct gctccaccag gtggcacgcg
     7681 acgcaggcga gcctcttcgc caggtcgaag ccgccggcgg cgccgtcggt cacggacagc
     7741 gcggccagcg cccgctgcgc gagatccttc gtctcctcgg cgctcgcgag cggcggccgc
     7801 gtcgtgtcgg cgccgcggac cggcccgacc acgcggtgct gcgtcatcag atttccgtgc
     7861 gcgtcgcgcg cccgctccgg gatcgccgac gtcaccttcg gctcggcgga gcagttccgc
     7921 atgcacgcgg tgcccgtcac gtcgggccgg cccgcgacgt cccacagtcc gtggtcgcgc
     7981 gtcatgcccg cgcggttcgg caggagccgc tgcacctcgc ggatgttcgc gtccgagagc
     8041 acgaagtcgg cggggacgat ctcgcccagg tgaaggatgt aggcgacgag ggcgtagacc
     8101 tcctcgaccg tcagcgtctt cggcgcgtgc cagggcatcg cgcggttgat gtagtcccac
     8161 agggtcgaca gcgtggagag cttcatgagc gtcgtgcgct gctccgaggg cgagcggagg
     8221 gcggcggcac ggccgctcgc gacgtctgcc ttcgtcgtgc ccccgacgat gggcgggaac
     8281 acctgggtgc tctcgccgaa ggtgccgtga caggaggcgc acttgtcgtc ccacgcctcc
     8341 tgccccctgg ccacgctgcc ggagccgcga ggcaggcccg tgaagtcggg gcgcacgtcg
     8401 atgtcccaca cctggacctc ggccggcgtg gccggacgcc cgacccccgg gaagcggccc
     8461 tgcgcgtgcg ccgctccggc gacgagcagg aggccggcca gcgcggccac gcctctagcc
     8521 gacctgaaca ttgctgacat cgccggcggc tgtcaccttc caagactgga tggcgttgtt
     8581 gtggtagatc gagcgggtgc cgcgcaccgc gcgcagctga gcgatggcgg gctgaacgta
     8641 tccggtctcg tcggcggccc gggactggag gatggcgtcc cgaccgtccc acacccagtc
     8701 caggctgaag cgtgtcaggg acttcgggag cacgggcgct tcgagccggg cgatgcgcca
     8761 gctccgtccg ccgtcgacgg acacgtccac ccgcttgacc cggccgcgcc ccgaccaggc
     8821 gagccccgtc acgttgtagt agccacgggc gaggagcacc tggccgccgg agggcgtcgt
     8881 gatgaccgac ttgcactcct ggatcgacgt gtactggcga tgccgcccgt ccggcatcag
     8941 gtcgacgtag tggaggacct cgtccttggt cccgtacggc tggtcgccga cctcgagccg
     9001 gcgcagccac ttcacccacg acacgccctg gacccccggc accacgagcc gaagcggata
     9061 cccgttctcg ggccgcagca tctcgccgtt catgccccag gcgacgatca cgtcgtcgag
     9121 cgcccgctcc atggcgatgg tccgcgtcag cccggagccg tcggcgccct cggcgagcac
     9181 gaacttgccc ttcttcagat cggccccgca gtcgtcgagc agcgtggcca gaggcacccc
     9241 cgtgaactcg cagcacgaga gcatcccgtg cgtgtactgg accgtgggga cggcgacgtt
     9301 gccccactcc atgcccgagt tcgcgccgca ctcgatgaag tggatgcgcg agacggcggg
     9361 cagccgcatg aggtcgtcca tggtgtagac cctggcggcc ttcacgaggc cgttgaccat
     9421 gaggcggtgc cggctcggat cgatgtcgtg ccagccctgg tgatgccgtt cgaagtgcag
     9481 cccgttgggc gtgatgatgc cgaagaaccc ctggagcggg gcgaaggaga cggaggcggc
     9541 atccacccgc gtcagccccg ggctctgccg ccgctgcagg ttcacctccc acttcgacgg
     9601 cacgccgtac ctgcgcgccg ccaccggctg cccgagcgag gcgctgtgcg gtggcagcgt
     9661 gaggatggcg ggatccccgg catccgccgc ggaggccgcg cgggcggcgg cgacagcggc
     9721 gacggcggcg aggcccgacg ccaggaaccc acgacggccc gagtcgatgg cggcgatctg
     9781 ccggggcccg aggaagctct cgggcgccgg gaggatgcgg ccgcggggaa tcagccggcg
     9841 cgccattgct gcctccgccg gcgcacgccc gcccgcgcca cctcgcggcg gcccgcgtcg
     9901 gccacccgcg cctgccggtc cagatgccgg gcgaggatct gccgcatggc cccgcacagg
     9961 accttgatgc cgggataggt cagccggaag tgcacctcgc ggccgtgccg tcgctcggtg
    10021 agcagatggc ccgcccggag gacgccgagg tgctgggaga ggttcgcctt gctcacgccc
    10081 gtctcgcgca ggagctccgg gtacgccacc tcgcggccct cgacgaggtc gaggatccgc
    10141 agccgcgtgg gatggccgag ggcccggacc atcttcgcct ggagctcgta cagctcgcgc
    10201 gagactgcgg ctcgcctggc ggtgcttcgg tttcgtcgtt gcaagatttc gaaacaatat
    10261 gcccgcgggc cggcgctgtc aagagtcggg gcgacgggcc gaagcgggcg ccgttgtctt
    10321 cggcgcttcc tcagtgccgg tgaccgtcga gggcctcgcc ccgcgccatc gccagatgat
    10381 ggggctggtg gtgcgcccct gcggtgatca gccccgcgaa ccccaggcca cggatgcggg
    10441 cgacgagctt cgcgtcgtcg gctcgcctgg tcgtgacggt cagacgcaca ccgcccgcga
    10501 tggtctccgt cctcgccgcg agcgaaggca tgcggtccag ctccaccgca tgcggcacca
    10561 ccatcgcgcg gatggcctgc tcggtgcgcc cggtgcccgt gatgtccatg ctgaggccgg
    10621 ccgggacggg tgtctgcctg accgtcgacc gcagcacgac ctcgctcatg tcgatgagat
    10681 gctggcggag tcgctcgagg tcgaccttgc tccagtcggt gttcggatcg gcctcgagga
    10741 tcgcgacgac ttccgcgatc gccccgaaag cgtcctggcc gggcagcgcg ggtgctccgc
    10801 ccccggccct gccgtgcatc gcctgcatgt gctgcgcgtg tgtgtcgggg tcgtgagacg
    10861 gcatcgaccc gtggtcgtgc ggctggcgcg gcccggaggc cggcgcctgc tgggccgaga
    10921 cggtgagcgc cagccccgct cccgcgaggg cgacgccgag gacgatggcg acggctgctc
    10981 tccacatgac tgcctcctgt tggaccgctc acccgccagt atcgagagcc gggcgcgccg
    11041 gatctatgac ctaagtcaca ccgccgccgc gtgccgcgtc ctcgtctccc cgggcgagcg
    11101 cggcgaggcg cgccggatcg cggatgacga tgcgcgagcg ctcgcgggag atgaccccgc
    11161 cctgctcgag ctgagagaag gcgcgcgcaa tgagctcgcg caccgtaccc agccgtgccg
    11221 cgagctgggc gtgggtcaga atgagctcga ccgaggctcc gggctcgacg ggtccgacga
    11281 ccgccgtggc cacgtagcgg gccaggcgct cggtcaccgg gcgaaaggcg aggtcgccga
    11341 cgatgcccgc gaagtgacgg acccgacggg ccatcgtctt cagcatggcc agggcgacct
    11401 cggggcgccg ccgacacagg ccgagcacct cggcgcgcgg caggaacagc acctgcgtcg
    11461 gcgcaagggc caccgccgag gcgatgtagc cgccgccgtc aaagagcggg ccttcgccga
    11521 gcgtggcgcc cggtccctcc gtgtggaaga cctgctcgcg tccccggagc gagatctgcc
    11581 ggatctcgac gcgtccttcg gcgacgacga ggaggccgcg gcacggttca ccttcctcga
    11641 agagggtctc cccagggccg agtctccgcg ccgtgcactg cccggccagg cggcgcacct
    11701 cggtcgccgg cagtgcagcg aagtaagaga cacggcggag gacctcgacg gtcgcggcaa
    11761 ccgacgcgcg aggaggagtc atggccggcg ctttagctca gctgtcgccg gaaccgccag
    11821 gcgctgacgc ccacgaggag actcgccagc gtcgccagcg cgaggaggtt cgggtagatc
    11881 acgtcgagcc cggcgccctt cacgagcacg ctgcgcgcga tcgtcgcgaa gtgagcgatg
    11941 gggttgagcg cggtcaaggg ctggacccag cccggcatgg cctcgatggg cgtcaaccct
    12001 ccggacagca tcgccagggg cggattcacg aagaagctga tcagctgcgt ctgggt
//
LOCUS       DATIAZ010000054         5108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_934032, whole genome shotgun sequence.
ACCESSION   DATIAZ010000054 DATIAZ010000000
VERSION     DATIAZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934032"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VLF19_01850"
     CDS             <1..761
                     /locus_tag="VLF19_01850"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HSE92021.1"
                     /translation="AGVLVKRFGRHEVDPLLRNIAVHAQYEGIPRAKGRRGGDIRVVT
                     RPDMGWLWLIPLSDTVTSVGAVIPRTVHGQSSKATAEESLAHYLADTPAAAPLFERAR
                     RVSAARFDADYSYLATRMAGDRWVAVGDAAAFLDPIFSTGVLLAMQGGLEAAEAIDAG
                     LRAGDLSRRAFAGYERIVRKRYGHFRRFAVGFYDPAFRDLWFVRPRRLVGVYRAVVSI
                     LAGNWRPSPMTRARVEVFFALVALRRFFRTVRRA"
     gene            880..1617
                     /locus_tag="VLF19_01855"
     CDS             880..1617
                     /locus_tag="VLF19_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HSE92022.1"
                     /translation="MTATAFGWLAQHGPRLYVGVLLVAMVDATGVPLPGRVLLIAAGA
                     LAHDWGQVAAMIGAATLGAVAGDHVWYMAGRVGSDRLMALYCKLSLVSGRCKRRARSH
                     LDRFGPLAIVIGRFFAGVRIASAPMVAIGAVSYPRYLVFEVIGAILWSAVFILLGHVL
                     GQPWRAIMGRFGVGTVLAVVGGLVVLGVAAIVALRLLRRRRHGQAETEHRGRRPGRRG
                     PAMAPTATSAARRPSRSSGLHPRGRRA"
     gene            1735..2505
                     /locus_tag="VLF19_01860"
     CDS             1735..2505
                     /locus_tag="VLF19_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSE92023.1"
                     /translation="MATTARINGRIPRYHRIAEALRSRIARGDLAPGARLPNQRRLAR
                     EFGVTLMTLRQALEVLEREKLIARHHGLGTFVAAPSIDYDINHLRRFAGDLLAQGEAV
                     TTRLLASRFVAADRRVAAALGLPPRARVLALERLRLVDGHPMSLQRSFLPAAIGEEVV
                     KADLAATPLRQALEFKLGLAVDRARETVSAVRLGRREARELGCRAGVPAFESERVSSA
                     ADGRPIVFDHVFIPGDRFRITRELKYDWHSDDLGGASA"
     gene            2502..3281
                     /locus_tag="VLF19_01865"
     CDS             2502..3281
                     /locus_tag="VLF19_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HSE92024.1"
                     /translation="MKILVAVKQVPDTATQVKIGADPRAIDTTGITWIVSPYDEFAVE
                     EALRIKEKRGQGEVVAVAVGPDRAKEALRSALAMGCDRAIHVSDPTLAEADTLTTARA
                     LAAVVKQEQPALVLCGRQAIDDDMGAVPAQLAELLGWPSAYWIMEDAVAADGKTIRSA
                     RQVEGGLEVFDIPLPAVLSAQKGLNEPRYPTLKGIMGAKKKEIKDLKAGDLGLGGEPP
                     QLSVVKLEALPPRPPGRVIPGDVSTAVKELVRALREDAKAI"
     gene            3311..4288
                     /locus_tag="VLF19_01870"
     CDS             3311..4288
                     /locus_tag="VLF19_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HSE92025.1"
                     /translation="MAGAIWCVVEDDRKGMPKKVMSEVLGEATRLGGPTEAVWITDKA
                     SDAGLAQLGEWGATKVWLLENAAFAPYRSEVWVAVAAELAGKESPRAILGPVTSRQRE
                     LLARLAARLGVGISADSTALAMEGDRLVATRPVYAGKLLAKVTWAKQPWIATLRPNVF
                     RPADVQAGRTAAVERPSVTVPGEQLKLVERREEASTGLPELTEAEIVISGGRGMKGPE
                     NYVILEELGGVIGAAVGASRAAVDAGWRPHRFQIGQTGRTISPKLYLGFGVSGAIQHL
                     AGMRTSKVIVAVNKDPEAPIFKIADYGIVADLFEVVPALTQEFKRLLEK"
     gene            4291..>5108
                     /locus_tag="VLF19_01875"
     CDS             4291..>5108
                     /locus_tag="VLF19_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92026.1"
                     /translation="MRLELTDEQQMVQAMAREFAESEIRPIAEEIDREARFPHETVKR
                     MGELGLMGIAIPERWGGSGADVVSYAVALVEIARQCASHAVVMSVNNSLYCEPVYKFG
                     TDAQRERFLTPFAQGHQIGCFALTEPQAGSDATNQHTLAVRDGDHYVLDGRKVFVTNG
                     REASVALVFCQTDRARAHRGISAFLVEKGTPGFHVPKTEDKLGIRASDTAEFVFEDCR
                     VPATNRLGEEGMGFKIAMGSLDGGRIGIAAQALGIAQGAYERAVAYAKERKAFGV"
BASE COUNT          713 a         1772 c         1876 g          747 t
ORIGIN      
        1 gggccggcgt gctcgtgaag agattcggcc gccacgaggt cgacccgctc ctgcgcaaca
       61 tcgcagtcca cgcccagtac gaaggaatcc cgcgcgccaa gggccgccgc ggcggtgaca
      121 tccgggtcgt cacgcggccc gatatgggct ggctgtggct gatcccgctg tcggacacgg
      181 tgacgagcgt gggggctgtg atccccagga cggttcatgg ccagagctcg aaggccaccg
      241 ccgaggaaag cctcgcgcac tacctggcgg acacgccggc ggccgcgcca ctcttcgagc
      301 gggcgcggcg cgtctccgcc gcccgcttcg acgccgacta ttcctatctg gcgacccgga
      361 tggcagggga ccgctgggtg gccgtcgggg atgcggcggc gttcctggac cccatctttt
      421 cgaccggtgt gttgctcgcc atgcaaggtg gtctggaagc cgccgaggcg atcgatgccg
      481 ggctgcgggc cggcgacctc tcgcggcgag ccttcgccgg atacgagcgc atcgtccgga
      541 agcgctacgg tcacttccgg aggttcgcgg tcgggttcta cgatcccgca ttccgtgatc
      601 tgtggttcgt ccgtcccagg cgcctcgtcg gcgtctaccg ggcggtcgtg tcgatcctcg
      661 ccggaaactg gcggccctcg ccgatgaccc gggccagggt cgaggtgttc ttcgcgctcg
      721 tcgccctgcg acgcttcttc aggacggtgc ggcgcgcgtg atcgcgtcac gtccctgacg
      781 cgccatggcg cgcgcgagtc gagatagcgg cggcgacacg gtcgagcgcc gggagccacg
      841 cgggctgccg ccaggccgca tactgtaggt gtggagacgg tgaccgccac cgcgttcggc
      901 tggctcgcgc agcacgggcc gcggctctac gtcggcgtct tgctggtcgc gatggtcgat
      961 gcgaccgggg tgccgctccc cgggcgcgtg ctgctgatcg ccgccggcgc cctggcgcac
     1021 gactgggggc aggtcgcggc gatgatcggg gccgccacgc tgggcgccgt cgccggcgac
     1081 cacgtctggt acatggccgg acgcgtgggc agcgaccggc tgatggcgct ctactgcaag
     1141 ctctcgctcg tctccggacg ctgcaagcgg cgcgcacggt cccacttgga ccggttcggc
     1201 ccgctcgcca tcgtcatcgg ccgcttcttc gccggggtga ggatcgcctc ggcccccatg
     1261 gtggcgatcg gcgccgtctc gtatccgcgc tacctggtgt tcgaggtgat cggggcaatc
     1321 ctctggtcgg cggtcttcat cctcctcggc cacgtgctgg gccagccgtg gcgcgcgatc
     1381 atgggccggt tcggggtcgg cacggtccta gccgtggtcg gtggcctcgt ggtcctgggc
     1441 gttgcggcga tcgttgccct ccgcctgctc cggcggaggc gccacggcca ggcggagacg
     1501 gaacaccggg gccggcgccc gggacgccgc ggcccggcaa tggctccgac tgcgacgtcg
     1561 gccgctcggc gacctagccg gtcctccggc ttgcaccctc gaggccggcg cgcctagaat
     1621 agctgcacga cgcgctcgag gtggattacg aggcgcgtcc aagcgcctgt cgctgtcatt
     1681 cgtcccgggg ttgacgatcg cgggatggtc ggcgagaagg agttctagaa caccgtggca
     1741 acgacggcac ggatcaacgg ccggatcccg cgctaccacc ggatcgccga ggctctgcgc
     1801 tcgcgcatcg cgcggggtga cctggcgccg ggcgcccggt tgccgaacca gcggcggctc
     1861 gcgcgcgagt tcggcgtgac gctgatgact ctccgccagg cgctcgaggt gctcgagcgc
     1921 gagaagttga tcgcccgcca tcacgggctc ggcacgttcg tcgccgcgcc gtcgatcgac
     1981 tacgacatca accacctgcg ccgcttcgcc ggcgacctgc tcgcgcaggg cgaggccgtc
     2041 accacgcgcc tgctggcgag ccgcttcgtc gccgccgatc gccgcgtcgc cgcagcgctc
     2101 ggcctgcccc cgcgcgcccg cgtgctcgcc ctcgagcggc tgcggctcgt ggacgggcat
     2161 cccatgagcc tgcagcgctc cttcctgccc gcggcgatcg gcgaggaggt tgtgaaggcc
     2221 gacctcgcgg ccacgccgct ccgtcaggcg ctggagttca agctcggcct ggccgtcgac
     2281 cgggcgcgcg agaccgtctc cgccgtgcgg ctcgggcgtc gggaggcccg cgagctcggc
     2341 tgccgcgccg gcgtgccggc cttcgagtcc gagcgcgtgt cgtccgccgc cgacgggcgc
     2401 cccatcgtgt tcgaccacgt cttcatcccg ggcgaccggt tccggatcac ccgcgagctc
     2461 aagtacgact ggcactccga cgaccttgga ggagcgtccg catgaagatc ctcgtggctg
     2521 tgaagcaagt tcccgatacg gcgacccagg tgaagatcgg cgccgacccg agagcgatcg
     2581 acacgaccgg catcacctgg atcgtatcgc cctacgacga gttcgccgtc gaggaagccc
     2641 tgcgcatcaa ggagaagcgc ggccagggcg aggtcgtcgc ggtggcggtc ggtcccgacc
     2701 gcgccaagga ggccctgcgc tcggcgctgg cgatgggctg cgaccgcgcg atccacgtca
     2761 gcgaccccac gcttgccgag gccgacacgc tgacgacggc gcgggcgctg gcggcggtgg
     2821 tgaagcagga gcagcccgcg ctcgtgctgt gcgggcgcca ggcgatcgac gacgacatgg
     2881 gcgcggtgcc cgcgcagctc gccgagctgc tgggctggcc cagcgcgtac tggatcatgg
     2941 aggacgccgt cgccgccgac ggcaagacga tccgctcggc gcgacaggtc gagggcgggc
     3001 tcgaggtctt cgacatcccg ctgcccgcgg tgctctcggc gcagaagggc ctcaacgagc
     3061 cgcgttatcc gacgctgaag ggcatcatgg gcgccaagaa gaaggagatc aaggatctga
     3121 aggccggcga tctcggactc ggcggcgagc cgccgcagct cagcgtcgtc aagctggagg
     3181 cgctgccgcc gcggccgccc gggcgggtca tccccggcga cgtgtcgacg gccgtcaaag
     3241 agctggtccg ggcgcttcgg gaggacgcaa aggcgatctg agtcgaccct ggggaggttt
     3301 cggaggagcc atggctggcg cgatctggtg cgtggtcgag gacgaccgga agggcatgcc
     3361 gaagaaggtg atgtcggagg tactcggcga ggccacgcgg ctcggcggcc cgacggaggc
     3421 ggtctggatc accgacaagg cctccgacgc cgggctcgcg cagctcggcg aatggggcgc
     3481 gacgaaagtg tggctgctgg agaacgccgc cttcgcgccg taccgcagcg aggtgtgggt
     3541 ggccgtggcc gcggagctgg ccggcaagga gtcgccccgg gccatcctcg ggcccgtgac
     3601 gagccgccag cgcgagctcc tcgcgcggct ggcggcgcgc ctgggcgtgg gcatctccgc
     3661 ggactccacg gcgctcgcga tggaagggga caggctcgtc gccacccgcc ccgtctacgc
     3721 gggcaagctg ctggcgaagg tgacgtgggc caagcagccg tggatcgcga cgctgcgtcc
     3781 caacgtgttc cgtccggctg acgtccaggc cggcaggacg gctgcggtcg agcggccgag
     3841 cgtgacggtt cccggggagc aactgaagct cgtcgagcgc cgcgaggagg cctcgacggg
     3901 cctgcccgag ctgaccgagg ccgagatcgt gatctcgggc ggacggggca tgaagggccc
     3961 cgagaactac gtgatcctcg aggagctggg cggcgtgatc ggcgctgccg tcggtgcctc
     4021 gcgggcggcc gtggacgccg gctggcgccc gcaccgcttc cagatcggcc agacgggccg
     4081 gaccatctcg cccaagctct acctgggctt cggcgtgtcg ggggcgatcc agcacctggc
     4141 gggcatgcgc acctcgaagg tcatcgtcgc cgtcaacaag gaccccgagg cgccgatctt
     4201 caagatcgca gactacggca tcgtcgccga tctcttcgag gtcgtgccgg cgctgaccca
     4261 ggaattcaag aggctgctgg agaagtgacg atgcgtctgg agctgaccga cgagcagcag
     4321 atggtccagg ccatggcgcg ggagttcgcc gagagcgaga tccggcccat cgccgaggag
     4381 atcgaccgcg aggcgcggtt cccgcacgag accgtcaagc gcatgggcga gctcggcctc
     4441 atgggcatcg ccatccccga gcggtggggc ggcagcgggg ccgacgtggt ctcctacgcg
     4501 gtcgcgctcg tcgagatcgc gcggcagtgc gcctcgcacg ccgtcgtcat gtccgtcaac
     4561 aactcgctct actgcgaacc ggtctacaag ttcggcaccg acgcccagcg cgagcggttc
     4621 ctcacgccgt tcgcccaggg ccaccagatc ggctgcttcg cgctcaccga gccgcaggcg
     4681 ggctcggacg ccaccaacca gcacacgctg gccgttcgcg acggcgacca ctacgtgctc
     4741 gacggccgca aggtgttcgt caccaacggg cgcgaggcgt cggtggcgct cgtcttctgc
     4801 cagaccgatc gcgccagggc ccaccgcggc atctcggcgt tcctcgtcga gaagggcacg
     4861 ccgggctttc acgtacccaa gaccgaggac aagctcggta tccgggcctc ggataccgcg
     4921 gagttcgtct tcgaggactg ccgcgtgccc gccaccaacc ggctcggcga ggagggcatg
     4981 gggttcaaga tcgccatggg gtcgctcgac ggcggccgca tcggcatcgc cgcgcaggcg
     5041 ctgggcatcg cgcagggcgc ctacgagcgc gcggtggcct acgccaagga gcgcaaggcg
     5101 ttcggcgt
//
LOCUS       DATIAZ010000055         8041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_803294, whole genome shotgun sequence.
ACCESSION   DATIAZ010000055 DATIAZ010000000
VERSION     DATIAZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803294"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VLF19_01880"
     CDS             complement(<1..467)
                     /locus_tag="VLF19_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92027.1"
                     /translation="MRGATRAGLASAFELETAPLAAEPPDDLLARCEALLAWRPLPRL
                     AARAPRLRWIHSLTAGVEQWLASPDLPRDAVLTCSRGVHRLSMPEHILAALFLVQKKL
                     AAIVLDQRERRWTRHVNDTLAGKTLGILGLGTIGAEVARKAHALEMRVIGTRRG"
     gene            complement(515..1738)
                     /gene="moeB"
                     /locus_tag="VLF19_01885"
     CDS             complement(515..1738)
                     /gene="moeB"
                     /locus_tag="VLF19_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(818..820),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HSE92028.1"
                     /translation="MKTLKEMITEARQAVPEEGPADVQRRLRAREPVVVIDVRDPDEY
                     RDGHIEGATNVSRGFLEFRVGSVVADQETPVVLYCQTGLRSVLAGKALKELGYKHVIN
                     LQGGYQKWAQSGLPTVREEPFTTDQIQRYSRHFLLPQVGEKGQHRLLRSRVLLIGAGG
                     LGSPTALYLAAAGVGTLGLMDGDTVDITNLQRQVLHTTADIGKPKVESGTRTLKALNP
                     DVNVIPLATRITVDNVMDTIKDYDLIVDGSDNFETRYLVNDACYLAGKTNVHGSIFQF
                     EGMATVFAPNEGPCYRCLYPTPPPPGLVPSUSEAGVLGVLPGVIGMVQATEAVKVLLG
                     IGEPLIGRLLTYDALGMRFREVKLRRDPNCPVCGSSPTITDLTIHMDGHGGACETTTN
                     GQPAPAGAPTSRAGG"
     gene            1786..2670
                     /gene="rfbA"
                     /locus_tag="VLF19_01890"
     CDS             1786..2670
                     /gene="rfbA"
                     /locus_tag="VLF19_01890"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HSE92029.1"
                     /translation="MKGIILAGGSGTRLYPLTRAVSKQLVPVYNKPMVYYPLSSLMLA
                     GIREILVITTPEDREAFRRLLADGRQLGLTIDYAEQPRPEGLAQAFVIGRDFVGSDAV
                     ALALGDNIFYGHGFPEYLRRAADRKVGATVFGYWVRDPERYGVVEFDRAGRAIGLEEK
                     PTAPRSSYAVTGLYFYDNAVLDIAAGLRPSARGELEITDVNLAYLRAGTLHVEVLGRG
                     IAWLDTGTHEALLQASTFIQTIEERQGLMVACLEEIAYAMGLITADDVRRTAEPMRNN
                     AYGQYLLRLLEGAARARA"
     gene            2820..3365
                     /locus_tag="VLF19_01895"
     CDS             2820..3365
                     /locus_tag="VLF19_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HSE92030.1"
                     /translation="MRGMELSPEAKRQFHVQSYGGAPRIHGVEIVDLRRHHDDGGAMT
                     ELLRVADGRAEALAGFTLRQVNFSEVEPGAVKAFHLHTRQTDVWYVPPSDRMLVVLAD
                     VRRGSPTEGAQMRFVLGGGASRLVRIPPGVAHGVRNVGAATGRVIYFVDVQFSPAPAE
                     CDEGRLPWDYLGGEIWEITRG"
     gene            3396..4412
                     /locus_tag="VLF19_01900"
     CDS             3396..4412
                     /locus_tag="VLF19_01900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HSE92031.1"
                     /translation="MRRRPFVAVALSLLLPLAAAASPHVREPRPHPKTIAGEPSYVRR
                     VAPSIVGLRVLADENAPSSARLGSRRFGSAAIFDVRGYAVTVTYVLMDAMSVEAQLRD
                     GRTVRARVVGLDLETGLGVVQLTGAGPWPAATLGQSRDAAPGMLTGTVGVDEDNDLVW
                     VAGAVQGVRRFSGFWEYMLDRALMVAPGSPAWGGSAIVDASGEIVGVASLRLGEPPHV
                     NVAIPMETFVPIKDELIAAGRVVSRRPRPWLGLYTQARRDGVFVDGFSPIGPAAQAGF
                     RRGDRIVGVNGVAVRSQEEFYEQLWRGQAGDVIKVAVERGDARHVIPVASIDRYRLLR
                     APGR"
     gene            4428..5834
                     /locus_tag="VLF19_01905"
     CDS             4428..5834
                     /locus_tag="VLF19_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSE92032.1"
                     /translation="MKGAADTYEGRGLIADPIHQYIQYTRPGGIPGEATEQDVIDTPW
                     VQRLRRIPQLQSARWVFPAAEHSRFQHSLGAMHLAGRLAQQLYPSLRAIFPDGPSGPL
                     LEELLRMAGLLHDVGHGPFGHFFDDNFLVDYELTHELVGQRIIRESLGGVLRALRRSP
                     SGAFNADEWIDPEWICYLMGKAYTQPLESHPKWLPFLKPLLSGVFTADNMDYVLRDAY
                     MCGVAVGPIDVERIIYYAFFSEKGLTLNRAGLGAFIMFLNARFYMYTNVYYHRTTRGI
                     DLHLKEIFRDTMRLAFPIDLRQELHPYLHLTEWTLLEEVGRWHHAEEPEKKALGAEWQ
                     QILERRLKWRMSHEVVLDLFEPKRGQAFLDAETVERRVRMFLPARLRDFPFRIDMALQ
                     DPRPLNPLKMGDRQIYVYDASRSEVAAEPLTELLKYLPGKVAQCRIFATHHEHDADLA
                     IALQRALEEEPSSIVTNV"
     gene            6176..6841
                     /locus_tag="VLF19_01910"
     CDS             6176..6841
                     /locus_tag="VLF19_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HSE92033.1"
                     /translation="MSMSWRFITCAALFVTCLLTANVIAAKLITVAGVVLPAAIVVFP
                     LSYVLADVLTEVWGYAATRRVIWLGFGCNVVMVAAIWAGGEIPPAPFWKGQGAYVEIL
                     GQAPRILVASFAAYLVGEFTNAFVLAKLKIATAGRWLWLRTIGSTVVGQAVDSLVFVG
                     LAFVGTVPLGVLVAIMAGQWVAKVLYEAAATPLTYAAVGWLKSREGADTFDYQTDFNP
                     MRL"
     gene            complement(6914..>8041)
                     /locus_tag="VLF19_01915"
     CDS             complement(6914..>8041)
                     /locus_tag="VLF19_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HSE92034.1"
                     /translation="CNNRFKEMYIVPQELLQPGRPYAHFLRYLAEHGYYGEGDVDTLV
                     ARRVESLRNPSGKSFDDHAPDGRWFRILRRRVAAGGTVTVMTDVTEQKAAERDLLEAT
                     RRTEEANKLISDKNRMLEALYSEIRDKNRQLEEQTAQIAEWNARLETRVSEQVAQIGQ
                     MSKLTRFLSPKVSDLIMSGDVDDPLKTRRREITVVFVDLRGFTGFTETAEPEEVMGVL
                     REYHAELGRAIVAYDGTIEHFAGDGVMILFNDPVPVENHELQAIRMALMMRDSVAALS
                     RGWKKRGYALGFGVGIAGGYATIGTIGFEGRLDYGAIGTVTNLAARLCGEARDDQVLI
                     SPRIFSKVEAHIEAQPVGELSLKGFHRPVFAHNVLGVRPEG"
BASE COUNT         1143 a         2816 c         2801 g         1281 t
ORIGIN      
        1 ccgcgccgcg tgccgatcac ccgcatctcg agcgcgtgcg ccttgcgggc tacctcggcg
       61 ccgatggtgc cgagccccag gatgccgagc gtcttgccgg ccagcgtgtc gttcacgtgg
      121 cgggtccagc ggcgctcgcg ctggtccagc acgatggcgg cgagcttctt ctggacgaga
      181 aagagcgccg ccaggatgtg ctcgggcatc gagaggcgat gcacgccccg ggagcaggtc
      241 agcacggcgt cgcgcggcag gtccggcgag gccagccact gctcgacacc ggcggtgagg
      301 gagtggatcc accgcagccg cggcgcgcgg gccgcgagcc gcgggagcgg gcgccaggcg
      361 agcagggcct cgcagcgcgc gagcaggtcg tcgggaggct cggcggccag cggcgccgtc
      421 tcgagctcga acgccgaggc gaggcccgcc cgcgtcgcgc cgcgcacgta ggcgccgggc
      481 tggtcgagcc agagcaggac ggagcgcgtg gtgatcagcc gcctgcgcgc gaggtcgggg
      541 cgccggccgg tgccggctgg ccgttcgtcg tggtctcgca ggcgccgccg tgcccgtcca
      601 tgtggatcgt cagatccgtg atggtgggcg agctgccgca gaccgggcag ttcgggtcgc
      661 gccggagctt cacctcgcgg aagcgcatgc cgagcgcgtc gtaggtgagc agccggccga
      721 tcagcggctc gccgatgccg agcagcacct tcaccgcctc cgtcgcctgt accatcccga
      781 tcactcccgg gagcacgccc aggacgccag cctcgcttca ggagggcacg agccccggcg
      841 gcggcggcgt ggggtagagg cagcggtagc acggcccctc gttgggcgcg aagaccgtgg
      901 ccatgccctc gaactgaaag atcgagccgt gcacgttggt cttgccggcg aggtagcagg
      961 cgtcgttgac gaggtagcgc gtctcgaagt tgtccgagcc gtcgacgatc aggtcgtagt
     1021 ccttgatggt gtccatcacg ttgtcgacgg tgatgcgcgt cgccagcggg atgacgttga
     1081 cgtcggggtt cagggccttc agcgtgcgcg tgccggactc gaccttgggc ttgccgatgt
     1141 cggccgtcgt gtggagcacc tgccgctgga ggttcgtgat gtccacggtg tcgccgtcca
     1201 tgagcccgag cgtgcccacg ccggcggcag ccaggtagag cgcggtcggc gagccgagcc
     1261 cgccggcgcc gatcaggagc accctgctgc gcagcagccg gtgctggccc ttctcgccca
     1321 cttgcggcaa gaggaagtgc cggctgtagc gctggatctg gtcggtcgtg aacggctcct
     1381 cgcgcacggt cggcaggccc gactgcgccc acttctggta gccgccctgg agattgatca
     1441 cgtgcttgta gcccagctcc ttgagcgcct tgccggcgag caccgagcgg agcccggtct
     1501 ggcagtagag gacgaccggc gtctcctggt cggccacgac gctgccgacg cggaactcga
     1561 ggaagccgcg gctgacgttg gtggcgccct cgatgtggcc gtcgcggtac tcgtcggggt
     1621 cgcgcacgtc gatgacgacg acgggctcgc gcgcgcgcag gcgtcgctgc acgtcggccg
     1681 gcccctcctc aggcaccgcc tgccgggcct cggtgatcat ctccttcagc gtcttcatgg
     1741 cgccgatgtt accgccattc cggggcgagt gctatattcg gggcggtgaa gggcatcatc
     1801 ctcgccggcg gctccggcac acggctctac cccctcacgc gcgcggtcag caagcagctc
     1861 gttcccgtct acaacaagcc gatggtgtac taccccctgt cctcgctgat gctcgccggc
     1921 atccgggaga ttctcgtcat cacgacaccc gaggaccggg aggccttccg ccggctgctc
     1981 gctgacgggc gccagctcgg gctgacgatc gactacgccg agcagccgcg cccggagggc
     2041 ctggcccagg ccttcgtcat cgggcgcgac ttcgtcggca gcgacgccgt ggcgctggcc
     2101 ctcggcgaca acatcttcta cggccacggc ttcccggagt acctgcggcg cgccgccgac
     2161 cggaaggtgg gcgccaccgt gttcggctac tgggtgcgcg accccgagcg ctacggcgtc
     2221 gtcgagttcg accgggccgg acgcgcgatc ggcctcgagg agaagccgac ggcgccccgc
     2281 tcgtcgtacg cggtgaccgg actctacttc tacgacaacg cggtcctcga catcgccgcg
     2341 gggctgcgtc cgtcggcccg cggcgagctc gagatcaccg acgtcaacct cgcctacctg
     2401 cgcgccggca cgctgcacgt ggaggtgctg ggccgcggca tcgcctggct cgacacgggc
     2461 acccacgagg cgctgctcca ggcgagcacg ttcatccaga cgatcgagga gcggcaggga
     2521 ctcatggtcg cctgcctcga ggagatcgcg tacgccatgg gcctgatcac cgcggacgac
     2581 gtgaggcgga ccgccgagcc catgcgcaac aacgcctacg gccagtatct gctgcgcctc
     2641 ctggaaggcg ccgcccgggc tcgcgcttag acgcccgggt cacgcccgcc cggcgccgca
     2701 gcgacgggga cgggtgcgca gccgccaacg tcgacaacgg tggatgcgtc tgactccggg
     2761 aggccaggag cgtgtcggag tcccgcgggg cttcccgcgg ccgtgggaag cctgttagga
     2821 tgcgcggaat ggaactgagc ccggaagcca agcggcagtt ccacgtgcag tcctatgggg
     2881 gcgctccccg catccacggt gtcgagatcg tggacctgag gcgccatcac gacgacggcg
     2941 gggccatgac cgagttgctc agggtcgcgg acggccgcgc cgaggccctg gccggcttca
     3001 cgctgaggca agtgaacttc agcgaggtcg agccgggcgc cgtgaaggcc tttcaccttc
     3061 acacgcggca gaccgacgtg tggtacgtgc cgccgtccga ccgcatgctg gtcgtgctgg
     3121 cggacgtccg caggggctcg ccgaccgagg gcgcgcagat gcgcttcgtg ctcgggggcg
     3181 gcgcctcgcg cctggtccgc atcccgcccg gcgtggcgca tggcgtccgc aacgtcggcg
     3241 ccgccaccgg ccgcgtgatc tacttcgtcg acgtgcagtt ctcgcccgcg cccgcggagt
     3301 gcgacgaggg ccggctccca tgggactacc tgggcggcga gatctgggag atcactcgcg
     3361 gatgaccgcc tgccgggcgg tgtgaggagg agcccttgag gcgtcgcccg ttcgtcgccg
     3421 tcgcgctgtc gctcctgctg ccgctcgccg cggcggcgag cccgcacgtc cgcgagccgc
     3481 ggccgcaccc caagacgatc gccggcgagc cgtcctacgt gcggcgcgtt gcgccgtcga
     3541 tcgtaggtct ccgcgtgctg gccgacgaga acgcgccgtc cagcgcccgg ctcggcagcc
     3601 ggcgcttcgg cagcgccgcc atcttcgacg tgcgcggcta cgcggtgacc gtgacctacg
     3661 tcctgatgga cgcgatgagc gtcgaggccc agctccgcga cggccgtacc gtgcgcgcgc
     3721 gcgtggtcgg cctcgacctc gagaccgggc tcggcgtcgt gcagctcacc ggcgccgggc
     3781 cgtggcccgc ggccacgctc gggcagtcgc gcgacgcggc gcccggcatg ctgacgggca
     3841 ccgtgggcgt ggacgaggac aacgacctgg tctgggtcgc cggcgccgtg cagggcgtgc
     3901 gccgcttctc gggcttctgg gagtacatgc tcgaccgcgc cctcatggtc gcgccgggca
     3961 gccccgcatg gggtggcagc gcgatcgtcg atgcctcggg cgagatcgtc ggcgtggcct
     4021 cgctccggct cggcgagccg cctcacgtca acgtcgcgat ccccatggag acgttcgtgc
     4081 cgatcaagga cgagctgatc gccgccggac gagtcgtgag ccgccgcccg cgcccgtggc
     4141 tcggactcta cacgcaggcc cggcgcgacg gcgtcttcgt ggacggcttc tcgcccatcg
     4201 gccccgccgc gcaggcgggc ttccgccgcg gggaccgcat cgtcggcgtc aacggcgtag
     4261 ccgtgcgctc gcaggaggag ttctacgagc agctctggcg gggccaggcc ggcgacgtga
     4321 tcaaggtcgc ggtggagcgc ggcgacgcac gacacgtcat ccccgtggcc tcgatcgatc
     4381 gctatcggtt gctgcgcgcg ccggggcgct gagataatcg gcgccagatg aaaggcgccg
     4441 ccgatacgta cgagggccgg ggcctcatcg ccgaccccat ccaccagtac atccagtaca
     4501 cgcgccccgg cggcatcccc ggcgaggcga ccgagcagga cgtcatcgac acgccgtggg
     4561 tgcagcgcct gcgccgcatc ccgcagctgc agtcggcgcg ctgggtgttc cctgccgccg
     4621 agcacagccg gtttcagcac tcgctggggg ccatgcacct ggccggccgc ctcgcgcagc
     4681 agctctaccc ctcgctccgc gcgatcttcc ccgacgggcc gtcggggccg ctgttggagg
     4741 agctgctgcg gatggccgga ctgttgcacg acgtcggcca cggaccgttc ggccatttct
     4801 tcgacgacaa cttcctcgtc gactacgagc tgacgcacga gctggtgggc cagcgcatca
     4861 tccgcgagtc gttggggggt gtgctccgcg ccctccgtcg gagcccgtcc ggcgcgttca
     4921 atgccgacga gtggatcgac ccggagtgga tctgctacct gatgggcaag gcctacacgc
     4981 agccgctcga gtcccacccg aagtggctgc cgttcctcaa gccgctgctc tccggcgtct
     5041 tcacggcgga caacatggac tacgtgctgc gcgacgctta catgtgcggc gtggcggtcg
     5101 ggcccatcga cgtcgagcgc atcatctact acgcgttctt ctcggagaag gggctcacgc
     5161 tcaaccgcgc cgggctgggc gcgttcatca tgttcctgaa cgcgcgcttc tacatgtaca
     5221 cgaacgtcta ctaccaccgc accacgcgcg ggatcgacct gcacctcaag gagatcttcc
     5281 gcgacacgat gcggctggcc tttccgatcg acctgcgcca ggagctgcac ccctacttgc
     5341 acctcaccga gtggacgctg ctcgaagagg tcggccgctg gcaccacgcc gaggagcccg
     5401 agaagaaggc gctgggcgcg gagtggcagc agatcctcga acggcggctc aagtggcgca
     5461 tgtcgcacga ggtcgtgctc gacctcttcg agcccaagcg ggggcaggct ttcctcgacg
     5521 cggagacggt cgagcgccgc gtgcggatgt tcctgcccgc gcggctgcgg gactttccct
     5581 tccggatcga catggcgctg caggacccgc gtccgctcaa cccgctcaag atgggcgacc
     5641 ggcagatcta cgtgtacgac gccagccgca gcgaggtggc cgcggagccg ctgacggagc
     5701 tgctcaagta cctgccgggc aaggtcgcgc agtgccgcat cttcgccacg caccacgagc
     5761 acgacgcgga tctcgccatc gcgctccagc gcgcgctcga ggaggagccc tcctcgatcg
     5821 tcacgaacgt ctgatgcggg cagcccgacg ctatcgtgac ccgagcctga cggtcggcgc
     5881 ctcgaactgc accgcccgcc caggcgggtg gccgccccgc tccgccccga catccggccc
     5941 gtccccgcca ggggcgcggg cgagggaaaa tcgccgacga tggcgtggac acttgtcgat
     6001 gccggtggcc gtctggactt gtcgcaccga gtcgatgacc ccggcgtggc cgcacgaggt
     6061 gatgacgacg agaccgcggt ccttcacgtg caggtggtcg cgtgctcgcc ctggaacgtg
     6121 tctggtgtga ggcccccccc attatgggga ccggagccca taccatctag aatccgtgag
     6181 catgtcctgg cgcttcatca cctgcgcggc gctcttcgtc acctgcttgc tgacggccaa
     6241 cgtcatcgcc gccaagctca tcacggtggc cggcgtggta ctgcccgcgg ccatcgtggt
     6301 cttcccgctc tcctacgtgc tcgcggacgt gctgacggag gtgtgggggt acgccgcaac
     6361 gcggcgggtg atctggctcg gcttcggctg caatgtggtg atggtggccg ccatatgggc
     6421 gggtggcgag atccctccgg cgccgttctg gaaggggcag ggagcctacg tggagatcct
     6481 cggccaggcg ccgcgcatcc tggtcgcgtc gttcgccgcc tacctggtgg gggagttcac
     6541 caacgccttc gtgctcgcca agctcaagat cgccacggcc ggccgctggc tctggctccg
     6601 gacgatcggc tctaccgtgg ttggccaggc ggtggactcg ctcgtcttcg tggggctggc
     6661 cttcgtcggc accgtcccgc tcggtgtcct ggtggcgatc atggcgggcc agtgggtcgc
     6721 caaggtcctc tacgaggccg cggccacgcc gctgacgtac gccgctgtcg ggtggctgaa
     6781 atcgcgcgag ggcgccgata cgttcgacta ccagaccgat ttcaacccga tgaggctctg
     6841 agaaccactg ctcggcgcga tagctcggcg gtggagtgct cagtccgagg ggcctcgggc
     6901 tggcaagaac ccgctagcct tccgggcgga cccccaagac gttgtgagcg aagaccggac
     6961 ggtggaagcc cttcagggac agctccccga ccggctgcgc ctcgatgtgc gcctcgacct
     7021 tcgagaagat gcgcggggag atcaggacct ggtcatctct ggcctcccca cacaggcggg
     7081 cggcgagatt ggtgaccgta ccgatcgcgc cgtagtcgag gcgaccctcg aagccgatgg
     7141 taccgatagt cgcgtagcct cccgcaatgc cgaccccgaa gccgagtgca tagccgcgct
     7201 tcttccatcc gcgggagagg gccgctaccg aatcgcgcat catgagcgcc atgcggatag
     7261 cctgcaattc gtgattttcg accgggaccg ggtcgttgaa caagatcatc acgccgtcgc
     7321 cggcgaagtg ctcgatggtt ccgtcatagg ccacgatcgc gcggcccagt tcggcgtggt
     7381 actcgcgcag cacacccatc acctcctccg gttccgccgt ctccgtaaag ccggtgaacc
     7441 cgcgcaagtc cacgaacacc accgtgatct cccgtcggcg cgttttcaga gggtcgtcga
     7501 catcgcccga catgatcagg tcgctcactt tgggcgagag aaaccgcgtg agcttggaca
     7561 tctgcccgat ctgcgccacc tgctcggaga cccgcgtttc gagccgcgca ttccattcgg
     7621 cgatctgcgc cgtctgctcc tccagttgtc ggttcttgtc ccggatctcg ctgtagaggg
     7681 cttcgagcat ccggttcttg tcgctgatga gcttgttcgc ttcctccgtg cggcgggtcg
     7741 cctcgaggag atcgcgctcg gccgcctttt gctcggtcac gtcggtcatc accgtgacgg
     7801 tgccacccgc cgccaccctg cgccgcagaa tgcgaaacca ccggccgtcg ggtgcgtgat
     7861 catcgaagct cttccccgac gggttgcgca ggctctccac ccgccgggca accagcgtat
     7921 cgacgtcgcc ctcgccgtag tagccgtgct cggccagata gcgcaggaag tgcgcgtacg
     7981 gacggcccgg ttgcagcaat tcctgcggca cgatgtacat ctccttgaag cggttgttgc
     8041 a
//
LOCUS       DATIAZ010000056         7712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_803325, whole genome shotgun sequence.
ACCESSION   DATIAZ010000056 DATIAZ010000000
VERSION     DATIAZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803325"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VLF19_01920"
     CDS             complement(<1..796)
                     /locus_tag="VLF19_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE92035.1"
                     /translation="MTKRTFTVEGFRLDSGRLLPEMTLAYETYGKLAPDGTNAILVTH
                     GFTSSQHAAGRYSPADDSPGWWDGLIGPGKTIDTDRYFVVCSNMLGSSYGSTAPASVN
                     PATGTRYGPDFPHITLRDMVTAQKALLEALGVRRLVAVAGPSFGGYQAFQWAVSYPEF
                     MRGIVAVVTAPKATSRQPADSLPRRFARDPGWNGGWHYDKGGIPATMAALRYDTLMRY
                     GANESLAARFPDRDARQARLRAMSEAWARDFDPNSMITLRKASLTFD"
     gene            complement(1008..2069)
                     /locus_tag="VLF19_01925"
     CDS             complement(1008..2069)
                     /locus_tag="VLF19_01925"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSE92036.1"
                     /translation="MVKTSNLRVESIRPLIPPGILLEELPLGEEGSRLVAGARDEVRA
                     VLDGRDDRLLVVVGPCSIHDPRAGLEYARRLRRLADDLARDLLVVMRVYFEKPRTTVG
                     WKGLINDPRLDGSFAINEGLRLARRFLLDLVDLRLPAGCEFLDPITPQFISDLVTWAA
                     IGARTTESQVHRELASGLSMPVGFKNGTDGGVQIAIDALWAAAHHHRFLGVTEQGLAG
                     IVSTSGNPDCHVILRGGASGPNYDAISVQKTLAALRDARLPARLMIDTSHGNSEKDHG
                     RQPLVARDIGAQLAQGEASIVGLMMESFLVEGRQDLRDPARLVFGQSITDACLSWEAT
                     VPVLEGLAESVRARRTTRS"
     gene            2094..3209
                     /locus_tag="VLF19_01930"
     CDS             2094..3209
                     /locus_tag="VLF19_01930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSE92037.1"
                     /translation="MTYVPPPDAWPSRPPAALGLRPDGLAAAVEYHRTHESRWRRDFL
                     TESGRYIGVSDEPPTADDVLGPVRHREGPNGIVVRGGYVAAEWGDTGRVDMSFSIAKS
                     YLAVLAGIALARGLVGSIDDPVRERVRDGGFESRQNRAITWRHLLQQTSEWQGTLWDK
                     PDSIDHNRDVGKSELGFSDKGTLRPMRPPGTFWEYNDVRVNRLSLALLRVFRRPLADV
                     LREAVMDPIGASRDWEWQPYRNSWVQIDGVRMPSVPGGSHWGGGLWMSTRDHARFGLL
                     VHRRGHWGDRELVPVAWIDEMRRPCPLNPQYGLLWWLNTGRAQMPSAPEPCYAARGAG
                     SNVVWIDPEHDLVVVVRWIDKASVDGFVRRVLEAVER"
     gene            3206..4144
                     /locus_tag="VLF19_01935"
     CDS             3206..4144
                     /locus_tag="VLF19_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSE92038.1"
                     /translation="MSANTGGAPLVGGVARVVGGVEIGGTEVVCAVGTGPDDLRAEAR
                     VPTTTPDETLARVVAFFADQPAHTRPVAVGVASFGPLDLEERSTTWGCITATTKPGWS
                     HTDVAGALRRGLRVPIAIDTDVNGAALGEHRWGAGTGLDPFVYVTVGTGIGGGGVIGG
                     RTMRGLVHPEMGHMRVPHDGTADPFPGVCRIHGDCLEGLASGRAMRERWGAPAEALPA
                     EHPAWPLEARYLALGLVNIVATLSPRRIAAGGGVAAQPVLLPMVRREVVGLLAGYVRA
                     PAVLEHVGDYIVPPLLGERAGVLGAMALALDAPTVG"
     gene            complement(4149..5063)
                     /gene="puuE"
                     /locus_tag="VLF19_01940"
     CDS             complement(4149..5063)
                     /gene="puuE"
                     /locus_tag="VLF19_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HSE92039.1"
                     /translation="MTSPYPRDLVGYGKHPPDPGWPGGARLALQIVLNYEEGGERAIL
                     HGDEESEAFLQEVPSMAPLRGVRNMQVESMYEYGSRAGFWRLLRLFAERGISISVFAV
                     AMALERHPEAAAAIVEAGHEVVSHGWRWIDYQFVDEAVERQHLRRAVESLTRTTGTRP
                     LGWYTGRTSPSTRRLVVEAGGFLYDADAYNDDLPYWESIGGTPHLIVPYTLDVNDMKF
                     SSIAGFTTGEQWLAHARDAFDVLYAEGAERPKMMSVGLHARLIGRPGRFAALARFLDH
                     VARHDRVWVCRRVDIARHWIAHHPYRAG"
     gene            complement(5090..6334)
                     /locus_tag="VLF19_01945"
     CDS             complement(5090..6334)
                     /locus_tag="VLF19_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSE92040.1"
                     /translation="MSAYRDLVPSARILLGPGPTMADPRVLRAMATPLLGQFDPEFTA
                     IMNEVMDLSRLVFRTVNARAFPVSGTGRAGLEAALVSLLEPGDRVVVGECGRFGLLLV
                     EIAERCGADVVAVRGEWGRAIEPDAIEAALRGGQTKLVAMIHGETSTGVLQPLPEIAR
                     AAHAHGALLMADCVVTLGGCEVAVDRWGLDVAVAGTQKCLSGPSGLAPVTYNGVAEQA
                     IAGRRTKVRGNYLDLGQLADYWSPGRFNHHTAPTSMVYGLREALRAVHEEGLEARFAR
                     HRLHGDALRAGIDALGLALFGKEAPERRLPFITPVVVPDGVDELRVRRRLLEDFGIEI
                     GAAFGPLQGKIWRIGTMGWSAQRHNVLLCLAALEAVLRAEGLEVPAGGGVDAALAHYR
                     AAGADSGARVDDSLGAGGIGRV"
     gene            6417..6956
                     /gene="uraD"
                     /locus_tag="VLF19_01950"
     CDS             6417..6956
                     /gene="uraD"
                     /locus_tag="VLF19_01950"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HSE92041.1"
                     /translation="MPSQEALTGRLTLAALNRMERARFTRALGAVFESSPWVAERAWA
                     ARPFASVDTLHRAMADVVRRAPRETQVALLRAHPDLAGKAAREGAMTASSVAEQSSAG
                     LDRLSDEESERFGRLNAAYRQTFGFPFIIAVRRHDKAGILAAFERRLGHTAEEEVEAA
                     LAEVFDITRMRLDALLSPA"
     gene            6959..7327
                     /gene="uraH"
                     /locus_tag="VLF19_01955"
     CDS             6959..7327
                     /gene="uraH"
                     /locus_tag="VLF19_01955"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HSE92042.1"
                     /translation="MGRGARTLTTHVLDTARGRPAAGLRVDLAVIEGDGRTRLVKTVA
                     TNADGRTDGPLLAEGEMQAGCYELVFHVGAYFSAAGLPVTDPPFLDAVPIRFAIAEPD
                     AHYHVPLLVSPYGYSTYRGS"
BASE COUNT         1033 a         2764 c         2861 g         1054 t
ORIGIN      
        1 cgtcgaacgt cagcgaggcc ttgcggagcg tgatcatcga gttcgggtcg aagtcgcggg
       61 cccaggcttc ggacatcgca cgcaggcgag cctggcgcgc gtcgcggtcc gggaagcggg
      121 cggccagcga ctcgttggcg ccgtagcgca tcagggtgtc gtagcgcaac gcggccatgg
      181 tggcggggat accgcccttg tcgtagtgcc agccgccgtt ccatccggga tcgcgggcga
      241 agcggcgggg cagggagtcg gcgggttggc ggctggtcgc cttgggcgcg gtgacgacgg
      301 cgacgatgcc ccgcatgaac tcggggtagc tcacggccca ctggaaggcc tggtagccgc
      361 cgaacgaggg cccggcgacg gccacgaggc gccgcacgcc gagcgcctcc agcagcgcct
      421 tctgcgcggt caccatgtcg cgcagggtga tgtggggaaa gtcgggcccg taccgcgtgc
      481 cggtcgcggg gttcacgctg gcgggcgcgg tcgagccgta cgacgagccg agcatgttcg
      541 agcacacgac gaagtagcgg tccgtgtcga tcgtcttgcc cggaccgatg agcccgtccc
      601 accatccggg tgagtcgtcc gccggcgagt agcggccggc ggcgtgctgg ctgctggtga
      661 aaccgtgggt gacgaggatc gcgttggtcc cgtcgggcgc gagcttgccg taggtctcgt
      721 aggcgagggt catctcgggc aggaggcggc cgctgtcgag ccggaagccc tcgaccgtga
      781 atgtgcgctt cgtcacggca tccagcggat caggcgtggt catcgcgggg aggctcccga
      841 ccggcgccga gagggtggcg ctggtccgag gcggcgcggt ggcacagccg gcaagggcca
      901 gcagcgccac cgcgagcgcg agtggacgac acgaggtcgt ggtcaggtct cgaggtccat
      961 gacggcgacc gtgtccggcg cgccggggtc gggcgtgccg gttggcatca gctgcgcgtc
     1021 gtgcggcgag cgcggacgct ctcggcgagg ccttcgagca cgggcacggt cgcctcccag
     1081 ctcaggcagg cgtcggtgat ggattggccg aagacgagcc gcgcggggtc tcgcaggtcc
     1141 tggcggccct ccaccaggaa gctctccatc atgagcccga cgatgctagc ctcgccctgc
     1201 gcgagctgcg cgccgatgtc gcgcgccacc agcggctggc ggccgtggtc cttctcgctg
     1261 ttgccgtggc tcgtgtcgat catgaggcgc gcgggcagcc gggcgtcacg aagggccgcc
     1321 agcgtcttct gcacgctgat cgcgtcgtag ttgggcccgc tggcgccgcc gcgcagtatg
     1381 acgtggcagt cggggttgcc gctcgtggag acgatgccgg cgaggccctg ctcggtcacg
     1441 ccgaggaacc ggtggtggtg cgcggccgcc cacagggcgt cgatggcgat ctgcacgccg
     1501 ccgtcggtgc cgttcttgaa gccgacgggc atcgagaggc ccgaggccag ctcgcggtgc
     1561 acctggctct ccgtcgtgcg cgcgccgatg gccgcccacg tgacgaggtc cgagatgaac
     1621 tgcggggtga tggggtcgag gaactcgcag cccgccggca gccgcagatc gacgaggtcg
     1681 agcaggaagc ggcgcgccag cctcagcccc tcgttgatgg cgaacgagcc gtcgaggcgc
     1741 gggtcgttga tgagcccctt ccagcccacc gtggtgcgcg gcttctcgaa gtacacgcgc
     1801 atgacgacga ggaggtcgcg ggccaggtcg tcggccagcc ggcggagccg gcgggcgtac
     1861 tcgaggccgg cacgcgggtc gtggatcgag caagggccga cgaccaccag caggcgatcg
     1921 tcccggccgt ccagcaccgc gcgcacctcg tcgcgcgcgc cggcgaccag ccgcgagccc
     1981 tcctccccca gcgggagctc ctcgagcagg atgcccggcg ggatcagggg ccggatgctc
     2041 tcgacgcgca ggttgctggt cttcaccagc cgctcattgt acatagaaac accatgacct
     2101 acgtgccccc gcccgatgcc tggccgtccc gcccgcccgc cgctctcggc ctccggcccg
     2161 acggcctcgc ggcggccgtc gagtaccacc ggacccacga gagccgctgg cgccgcgact
     2221 tcctcaccga gtccgggcgc tacatcggcg tgtccgacga gccgccgacc gcggacgacg
     2281 tgctcggccc ggtgcgccac cgcgagggcc caaatggaat cgtcgtgcgc ggcggctacg
     2341 tcgccgcgga gtggggcgac acgggcagag tcgacatgtc gttcagcatc gccaagagct
     2401 acctggcggt gctcgccggc atcgcgctgg cgcgtgggct cgtcgggagc atcgacgatc
     2461 ccgtacgcga gcgcgtccgc gacggcggat tcgagtcccg gcagaaccgg gcgatcacgt
     2521 ggcggcattt gctccagcag acgagcgagt ggcaggggac gctctgggac aagcccgact
     2581 ccatcgacca caaccgcgac gtcggcaaga gcgagctcgg tttctcggac aaggggaccc
     2641 tgcgcccgat gcggccgccg ggcacgttct gggagtacaa cgacgtgcgg gtgaaccggt
     2701 tgagcctcgc gctgctgcgg gtgttccggc gcccgctggc cgacgtgctg cgcgaggccg
     2761 tgatggaccc catcggcgcc tcgcgcgact gggagtggca gccctaccgg aattcctggg
     2821 tccagatcga cggtgtccgc atgccgtcgg tgccgggcgg ctcgcactgg ggtggggggc
     2881 tgtggatgag cacgcgggac cacgcgcggt tcggcctgct cgtccaccgg cgcgggcact
     2941 ggggcgaccg cgagctcgtc cccgtcgcct ggatcgacga gatgcgccgg ccgtgcccgc
     3001 tcaatcctca gtacgggctg ctctggtggc tcaacaccgg ccgcgcccag atgccgagcg
     3061 cgcccgagcc ctgctacgcc gcgcgcggcg ccggcagcaa cgtcgtctgg atcgaccccg
     3121 agcacgacct ggtcgtcgtc gtgcgctgga tcgacaaggc gagcgtggac ggcttcgtgc
     3181 gccgggtgct ggaggccgtc gagcgctgag cgcgaacacc ggcggcgcac ctctcgtcgg
     3241 cggtgtcgcg cgggtcgtcg gcggggtcga gatcggtggc accgaggtcg tctgcgccgt
     3301 cgggaccggg cccgacgatc tccgcgccga ggcccgcgtg ccgacgacga ccccggacga
     3361 gaccctcgcg cgcgtcgtcg ccttcttcgc cgaccagcca gctcacacgc gacccgtcgc
     3421 ggtcggcgtc gcgtcgttcg gtcccctgga tctcgaagag cgctccacca cgtggggctg
     3481 catcaccgcc accaccaagc cgggctggtc gcacaccgac gtggcgggcg ccctgcgccg
     3541 ggggctacgt gtcccgatcg ccatcgacac cgacgtcaac ggcgccgccc tcggtgagca
     3601 ccgatggggt gccgggaccg ggctcgaccc cttcgtctac gtgaccgtcg ggacggggat
     3661 cggtggcggt ggcgtcatcg ggggccggac gatgcgaggg ctcgtccatc cggagatggg
     3721 gcacatgcgc gtgcctcacg acggcaccgc cgacccgttc cccggcgtct gccggattca
     3781 cggcgattgc ctcgagggcc tcgcctcggg ccgcgcgatg cgcgagcggt ggggcgcgcc
     3841 ggcggaggcg ctgccggccg agcatcccgc gtggccgctc gaggcgcgct acctggcgct
     3901 ggggctggtg aacatcgtcg ctacgctgtc gccccgccgc atcgccgccg gcgggggggt
     3961 cgccgcgcag cccgtgctcc tgccgatggt ccgccgcgag gtggtcggcc tgctcgccgg
     4021 ctacgtccgg gcgccggcgg tgctcgagca cgtcggcgac tacatcgtcc ccccgctgct
     4081 gggtgagcgc gccggcgtgc tcggggcgat ggcgctggcc ctggacgcgc cgacggtcgg
     4141 ctgagcgatc accccgcgcg atacggatgg tgcgcgatcc agtggcgggc gatgtccacg
     4201 cggcggcaga cccacacgcg gtcgtggcga gccacgtgat cgaggaagcg ggcgagcgcc
     4261 gcgaaccggc cgggacggcc gatgagccgc gcgtgcaggc ccaccgacat catcttcggg
     4321 cgctcggccc cctcggcgta gagcacgtcg aaggcatccc gcgcgtgggc cagccactgc
     4381 tcgccggtgg tgaatccggc gatcgacgag aacttcatgt cgttgacgtc gagcgtgtag
     4441 ggcacgatca ggtgcggtgt gcctccgatg ctctcccagt acggaaggtc gtcgttgtag
     4501 gcgtcggcgt cgtagaggaa gccgcccgcc tcgaccacga gccgccgcgt gctggggctg
     4561 gtgcgccccg tgtaccagcc gagcgggcgc gtccccgtcg tccgcgtgag gctctcgacc
     4621 gcccgccgca ggtgctggcg ctcgaccgcc tcgtcgacga actggtagtc gatccaccgc
     4681 cagccgtggc tcaccacctc gtggcccgct tcgacgatgg ccgccgccgc ctcgggatgg
     4741 cgctcgagcg ccatcgccac cgcgaagacg ctgatgctga tcccacgctc ggcgaagaga
     4801 cgaaggagcc gccagaagcc ggcgcggctg ccgtactcgt acatcgactc gacctgcatg
     4861 ttccgcacgc cgcgcagggg cgccatgctc ggcacctcct gcaggaacgc ctcggactcc
     4921 tcgtcaccgt gcaggatcgc gcgctcaccg ccctcctcgt agttgaggac gatctggagc
     4981 gccagccgcg cgccgccggg ccacccggga tcgggcgggt gcttgccgta gccgacgagg
     5041 tcgcgcggat agggcgaggt cacgtcagcg tgagctggaa gacggcacgt cagacgcggc
     5101 cgatgccgcc ggcccccagg ctgtcgtcga cgcgcgcgcc gctgtcggcg ccagcggccc
     5161 ggtagtgagc gagggcggcg tcgacgccgc cgcccgccgg cacctcgagg ccctccgcgc
     5221 gcaggacggc ctccagcgcg gcgaggcaca ggagcacgtt gtggcgctgg gcggaccagc
     5281 ccatcgtgcc gatgcgccag atcttgccct ggagtggacc gaacgccgcg ccgatctcga
     5341 tgccgaagtc ctcgaggagc cggcggcgca cgcgcagctc gtccacgccg tcgggcacga
     5401 cgaccggcgt gatgaagggc aggcggcgct ccggcgcctc cttgccgaac agggcgagac
     5461 ccagcgcgtc gataccggcg cgcagcgcgt cgccgtgcag gcggtggcgg gcgaagcgtg
     5521 cctcgagccc ttcctcgtgg acggcgcgca gcgcctcgcg caggccgtag accatcgacg
     5581 tcggcgccgt gtggtggttg aagcgccccg ggctccagta gtccgcgagc tgtccgaggt
     5641 cgagatagtt gccgcgtact ttcgttcgcc gcccggcgat agcctgctcc gccaccccgt
     5701 tgtaggtcac cggcgcgagc cccgaggggc cggagagaca cttctgcgtg cccgcgacgg
     5761 cgacgtcgag cccccagcgg tccacggcga cctcgcagcc gcccagcgtg acgacgcagt
     5821 cggccatcag tagcgcgccg tgcgcgtggg ccgcccgcgc gatctccggg agcggctgaa
     5881 ggacacccgt ggacgtctcg ccgtggatca tcgccacgag tttcgtctgg ccgccgcgca
     5941 gcgccgcctc gatcgcgtcc ggctcgatgg cgcgccccca ctcgccgcgg acggcgacga
     6001 cgtcggcgcc gcagcgctcg gcgatctcga cgagcaggag cccgaacctg ccgcactcgc
     6061 ccacgaccac tcgatctccc ggctcgagca acgacaccag cgcggcctcc aggcccgcac
     6121 gcccggtgcc cgacaccggg aaggcgcgtg cgttgaccgt ccggaagacc aggcgcgaca
     6181 ggtccatcac ctcgttcatg atcgcggtga actcggggtc gaactggccc aggagcggcg
     6241 tggccatcgc gcggagcacg cgtgggtcgg ccatcgtcgg gccagggccg agcaggatcc
     6301 gcgcggacgg caccagatcc cggtacgcgg acatgccccg accttacccg attcgcctcg
     6361 ccgcgggccg gaaaatcggc ttgccggagg gcggaaaatc ggccagagtt cgccacgtgc
     6421 cgtcccagga ggctctcacc ggccggctca cgctggccgc gctcaatcgc atggagcggg
     6481 cccgcttcac gcgcgcgctg ggggcggtct tcgagagctc gccgtgggtg gccgagcggg
     6541 cgtgggcggc gcggcccttc gcgagcgtgg acaccctgca ccgggccatg gccgacgtgg
     6601 tccgccgcgc gccgcgcgag acgcaggtgg cgctgctgcg cgcgcacccg gacctcgccg
     6661 gcaaggcggc gcgtgaaggc gccatgaccg cctcgtcggt cgccgagcag tcctccgcgg
     6721 gcctcgaccg gctcagcgac gaggagagcg agcgtttcgg ccgcctcaac gccgcctacc
     6781 gccagacgtt cggctttccc ttcatcatcg ccgtgcgccg tcacgacaag gccggcatcc
     6841 tcgccgcgtt cgagcggcgc ctcggccaca ccgccgagga ggaagtggag gcggccctgg
     6901 ccgaggtatt cgacatcaca cgcatgcgcc tcgacgcgct cctgagcccc gcgtgagcgt
     6961 ggggcggggc gcgcgcacgc tgacgacgca cgtgctcgac accgcgcgcg ggcggccggc
     7021 cgccgggctg cgcgtggacc tcgcggtcat cgagggcgac ggccgcacgc ggctcgtcaa
     7081 gaccgtcgcg accaacgctg acgggcgcac cgacggcccg ctgctcgcgg agggcgagat
     7141 gcaggcggga tgctacgagc tcgtcttcca cgtgggcgcc tacttctccg ccgccggcct
     7201 gcccgtgacg gatccgccgt tcctggacgc ggtccccatc cgcttcgcca tcgccgagcc
     7261 ggacgcgcac taccacgtgc cgctgctggt ctcgccgtac ggatactcga cgtaccgcgg
     7321 gagctgagcg cggtcacgct ggaggcgtta acccatcgct tcggcgccgt caccgcggtg
     7381 gacgacgtca cgctggacat ccgcaccgac gagctgctcg cgcggcggcg gcgtcagggc
     7441 tgctgatcct cttcaccctg gtcgtgctgc tggccgccga gcggcttgcc ggtctgacgc
     7501 ccagctacgg cgacggtcga ccatgccgtt ggcgtcgcgg ccctcgcggc cgctacgtgg
     7561 ctggcggcgg tcggcgggta ccccagacga agagatgggt ctggccgccg aggaagcgtc
     7621 cggcggcctg ctcggcgcgg agggccgcca gccactcgcg ctgctcgtcc acggtgatgg
     7681 cgccggcctg cacggcgacg gcagcgcggc gc
//
LOCUS       DATIAZ010000057         5646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1460612, whole genome shotgun sequence.
ACCESSION   DATIAZ010000057 DATIAZ010000000
VERSION     DATIAZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460612"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VLF19_01960"
     CDS             complement(<1..734)
                     /locus_tag="VLF19_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92043.1"
                     /translation="MDSGHHVTDRVAIVTGAGHGIGRATALALAGAGADVAMVDIDQA
                     AAEKTAAAVTALGRRSLAVGADVGDVAAIDRMVRQVTDAFGRIDVLVNNAGVTRRAYI
                     MDLTEADWDRIMRVNGKGVFFCLQRVAREMIPRRSGVIVNIASIAGKGYVGASNAIYA
                     ASKAAVIGMTRMAALQLARHNINVNAICPGTTVTALSNDNLRNRAREEGVSVEEMERR
                     RNSAIPLGRPNDPDDVAALAVFLASPG"
     gene            885..2153
                     /locus_tag="VLF19_01965"
     CDS             885..2153
                     /locus_tag="VLF19_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92044.1"
                     /translation="MIVSRRNFLQLAGAAALAAGSRLGAAPAFAQGGGPVKIGVLTIR
                     AGIAAPVGTAGLRGTEWWAERVNKAGGILGRPVQLVVEEESSPKDTVERYRKLVLQEK
                     VEVVLGGISTGVTLALGPVAEDLATPWLSWDGTTQKGVEETMPNPRWAFKSVDNEVEA
                     IAAGILTAKYFKGIKTIAGINNDYSYGHDCWQSYQAVLKRYGFDVKPVLELFPKLGVT
                     DFTSHIASIQQAKPDLLMTSFWSGDATILMKQAAAVGLFKTMKGVFTTAGGVHDSLKK
                     EFTPEGLLLGYNSMYFEDPKGSALLKQFVREYKAKYNEYPPYECDHAYFCAESYRAAA
                     EKAAGSRKQWPEKAAVVKALEGIEVESISGKRSWREDHVQMCNFYQGITTHKNGYDFV
                     TINPVEVVSTRQAMKPAGTKLFDWISGWKI"
     gene            2226..3113
                     /locus_tag="VLF19_01970"
     CDS             2226..3113
                     /locus_tag="VLF19_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92045.1"
                     /translation="MTSDLVNIVMSGVFHASILFLVAAGLQVVFGVQKIFNLACGSFY
                     ALGAYVGVSAVQAFVDAGGPPALFVLPLALAGLGVGVVGVVVERGLLRFVYGRDETFQ
                     LLLTFALVLMLEDVVRMTWGTAPRSTAGLYLTYGQVRVLGAAVPVYNLIVIAASVLIA
                     VTIGWLLTRTAFGRIIRATADNREMAEALGVDMRRVYAKVFTLGTALGTLGGALVIPA
                     TAAMSEMGVELIVEAFAVVVIGGLGSMRGALVGAMVVGILRAVAISVYPEIEMLLIYL
                     IVITVLVLRPRGLFGAPAA"
     gene            3110..4066
                     /locus_tag="VLF19_01975"
     CDS             3110..4066
                     /locus_tag="VLF19_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92046.1"
                     /translation="MTARVVVAAATLAVAVAPLVMPTYHTLLMLPFMAYGVILLGLNL
                     LFGYTGLVSFGHALFIGVGAYAGAFLTTHTSLRSLEVILVVSAVLGAVVAAPVGALCV
                     RYVKIYFGMLTLAFGMVFYTFLLKFYRLTGGDEGMRVLRPTLLGRDFADLSKTDYLVG
                     PYYFYSLGVLVVAAVVMWRIVGSPFGLCLRTVRDNPMKAETLGISVTRYRWYAFVISA
                     VYAAVGGALLGPPTGNVDPTLAYWTHSGNIVFMTLLGGFASFFGPVVGAFVFIYLQDY
                     VMSVVPYWRLVFGAILAVVVIFAPGGLMGFFPPRRPAAPLRP"
     gene            4063..4821
                     /locus_tag="VLF19_01980"
     CDS             4063..4821
                     /locus_tag="VLF19_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92047.1"
                     /translation="MILSAEGIRKHYGAFCALDGVSLGVRAGEFVSIIGPNGAGKSTL
                     INVLTGVLAPTAGTVRFKDRDIAGIGPVRLARLGMARSFQLVHVFPELTVLETLQAAA
                     VSRLGRSRRLFASLARDAEVRDAALEVAELFGLADKRHARSKHLPQGDKKLLDVASAF
                     ALRPEIILLDEPTSGVSTRDKTAIMEILVAAAKRIGLQAIIQVEHDMDIVFAYSDRIV
                     ALHQGRVLADATPAQIQADQRVVDTVIGRRAATL"
     gene            4833..5540
                     /locus_tag="VLF19_01985"
     CDS             4833..5540
                     /locus_tag="VLF19_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92048.1"
                     /translation="MLSVTGVDVFIETSQILREVSLEVGEREVVCLVGRNGAGKTTAL
                     RTIMGYLRPRAGRITFRDRPIHGRRTHEIARLGLGFAPEDGAIFGDLTVAENIEIATW
                     TRPGGRPAPERLALAYDVFPNLRQYARRKGPQMSGGERKMLSIARALALDPEMLLLDE
                     PFEGLSPAIIPTIAEGLATITSLGHAILLAESNIHHVPVWATRLYVIERGEIVFAGTP
                     AAARRDPAVGRIIGGST"
BASE COUNT          777 a         2024 c         1921 g          924 t
ORIGIN      
        1 cccggcgagg ccaggaacac ggcgagggcg gccacgtcgt cggggtcgtt gggccgcccc
       61 agcggaatgg cggagttccg ccggcgctcc atttcttcga cgctgacgcc ctcctcacgg
      121 gcgcgatttc gcagattgtc gttggacagc gcggtcacgg tcgtgccggg gcagatggcg
      181 ttgacgttga tgttgtgacg cgcgagctgg agcgcggcca tgcgcgtcat cccgatcacg
      241 gcggccttgc tggcggcgta gatggcgttc gacgcgccca cgtacccctt gccggcgatg
      301 gaggcgatgt tgacgatcac gccgctccgg cgggggatca tctcgcgcgc cacgcgctgc
      361 aggcagaaga agacgccctt gccgttgacg cgcatgatgc ggtcccagtc ggcctcggtg
      421 aggtccatga tgtacgcgcg tcgcgtgacg cctgcattgt tcacgaggac gtcgatccgg
      481 ccgaaggcat cggtgacctg gcggaccatc cggtcgatgg cggcgacgtc gccgacgtcg
      541 gcgccgacgg ccaggctccg gcgcccgagc gcagtgaccg ccgccgccgt cttctcggcc
      601 gccgcctggt cgatgtcgac catcgcgacg tcggcgccgg ccccggccag cgccagcgcg
      661 gtggcgcgcc cgatgccgtg cccggcgccc gtgacgatgg cgacgcgatc ggtcacgtga
      721 tggccgctgt ccatggcgag agcatgatac tgcacgtcca gaggcgaggc gccagcgaag
      781 cggtgggcct ttttcggcag cccacgcccg gaaccgacta gaatgtgcca cgtcgtccgg
      841 accactcgcg tccgcgcacc cgatcgttcg aggaggggtc aacgatgatc gtcagtcggc
      901 ggaatttcct gcagctcgcc ggcgccgccg ccctcgctgc gggcagccgt ctcggcgccg
      961 cgccggcctt cgcccagggc ggaggccccg tgaagatcgg tgtgctcacc atccgcgccg
     1021 gcatcgcggc gccggtgggc acggccggac tccggggcac ggagtggtgg gccgagcgcg
     1081 tcaacaaggc gggcggcatc ctgggccggc ccgtgcagct ggtggtcgag gaggaatcga
     1141 gccccaagga caccgtcgag cgctatcgca agctcgtgct gcaggagaag gtcgaggtcg
     1201 tcctcggcgg catctccacc ggcgtgacgc tcgcgctcgg gcccgtcgcc gaggacctgg
     1261 ccacgccgtg gctgtcgtgg gacggcacca cgcagaaggg cgtcgaggag acgatgccca
     1321 atccccggtg ggccttcaag agcgtggaca acgaggtcga ggccatcgcg gcgggcatcc
     1381 tcaccgcgaa gtacttcaag ggcatcaaga ccattgccgg catcaacaac gactactcct
     1441 acggccacga ctgctggcag tcctaccagg cggtgctcaa gcggtacggc ttcgacgtca
     1501 agcccgtgct cgagctcttc cccaagctcg gggtgacgga cttcacctcg cacatcgcct
     1561 ccatccagca ggccaagccg gacctcctca tgacgtcgtt ctggtccggc gacgccacca
     1621 tcctcatgaa gcaggcggcg gcggtcgggc tcttcaagac catgaagggc gtcttcacca
     1681 ccgccggcgg cgtccacgac tcgctcaaga aggagttcac gcccgagggg ctgctgctcg
     1741 gctacaactc gatgtacttc gaggatccca agggcagcgc cctgctcaag cagttcgtcc
     1801 gcgagtacaa ggcgaagtac aacgagtacc cgccctacga atgcgaccac gcctatttct
     1861 gcgccgagtc ctacagggcc gccgcggaga aggcggcggg ctcgcgcaag cagtggccgg
     1921 agaaagcggc cgtggtcaag gcgctcgagg gcatcgaggt ggagtcgatc tcgggcaagc
     1981 ggagctggcg tgaggatcac gtccagatgt gcaacttcta ccagggcatc acgacccaca
     2041 agaacggcta cgacttcgtg accatcaatc ccgtcgaggt cgtctccacc cggcaggcca
     2101 tgaagcccgc gggcaccaag ctcttcgact ggatcagcgg ctggaagatc tgatcgacgg
     2161 atcgcggggc aggcgcggat gatcgcgcgc gtgcggcgcc tgcccccgtc cacccccctg
     2221 cgcccgtgac gtccgacctc gtcaacatcg tcatgagcgg cgtgttccac gcgtcgatcc
     2281 tgttcctggt ggcggccggg ctgcaggtgg tgttcggcgt ccagaagatc ttcaacctcg
     2341 cctgcggctc gttctacgcg ctgggcgcct acgtcggcgt ctcggcggtg caggccttcg
     2401 tcgacgccgg cgggccgccg gcgctgttcg tgctgccgct cgcgctggcg ggcctcgggg
     2461 tcggcgtcgt gggcgtggtc gtcgagcgcg ggctgcttcg cttcgtctac ggccgcgacg
     2521 agaccttcca gctgctcctg accttcgccc tcgtgctcat gctggaggac gtcgtccgga
     2581 tgacgtgggg cacggcgccc cggtccaccg caggcctcta tctcacgtac ggccaggtgc
     2641 gtgttctcgg cgcggccgtg cccgtgtaca acctgatcgt catcgcggcc agcgtcctca
     2701 tcgccgtcac catcggctgg ctgctcacgc gcaccgcgtt cggccggatc atccgtgcga
     2761 ccgccgacaa ccgcgagatg gccgaggcgc tcggcgtcga catgcgtcgg gtctacgcga
     2821 aggtcttcac gctcggcacc gcgctgggca ctctcggggg tgccctggtc atcccggcca
     2881 cggccgcgat gagcgagatg ggggtcgagc tgatcgtcga ggccttcgcc gtcgtcgtca
     2941 tcggcggtct cggcagcatg cgaggcgcgc tggtcggcgc gatggtcgtc gggatcctcc
     3001 gcgccgtcgc catctccgtc taccccgaga tcgagatgct cctcatctat ctgatcgtca
     3061 tcacggtgct ggtgctccgc ccccgcgggc tcttcggggc gcccgcggca tgaccgctcg
     3121 cgtggtcgtg gccgcggcca ccctcgccgt ggccgtcgcg ccgctggtga tgccgaccta
     3181 ccacacgctg ctcatgctgc ccttcatggc ctacggcgtc atcctgctcg ggctgaacct
     3241 cctgttcggc tacaccggcc tggtgtcgtt cggccacgcg ctcttcatcg gcgtcggcgc
     3301 ctacgcgggc gccttcctca cgacgcacac cagcctgcgc tcgctggagg tcatcctggt
     3361 cgtgtcggcc gtgctcggcg cggtcgtcgc cgcgcccgtc ggcgccctgt gcgtgcggta
     3421 cgtcaagatc tacttcggca tgctgacgct ggccttcggc atggtcttct acacgttcct
     3481 gctcaagttc taccgcctga ccggcggcga cgaggggatg cgggtgctgc ggccgacgct
     3541 gctcgggcgg gacttcgcgg acctgtccaa gaccgactac ctcgtggggc cctactactt
     3601 ctactcgctc ggcgtgctcg tcgtcgccgc cgtcgtgatg tggcgcatcg tcggctcgcc
     3661 cttcggcctg tgcctgcgca ccgtccggga caacccgatg aaggcggaga cgctcggcat
     3721 ctccgtcacc cgctaccgct ggtacgcctt cgtgatctcc gccgtctacg ccgccgtcgg
     3781 cggcgcgctc ctgggcccgc ccaccggcaa cgtggacccg acgctggcgt actggacgca
     3841 ctcgggcaac atcgtcttca tgacgctcct gggcggcttc gccagcttct tcggccccgt
     3901 ggtcggcgcc ttcgtcttca tctacctgca ggactacgtc atgtcggtcg tgccgtactg
     3961 gcggctggtc ttcggcgcca tcctggccgt cgtggtgatc ttcgcgccgg gcgggctgat
     4021 gggcttcttc ccgccgcggc gcccggcggc gccgctgcgc ccatgatcct gagcgccgag
     4081 gggatccgga agcactacgg cgccttttgc gccctcgacg gggtcagcct cggcgtccgg
     4141 gccggcgagt tcgtctcgat catcggcccc aacggcgcgg gcaagtcgac gctgatcaac
     4201 gtcctgacgg gcgtgctggc gccgaccgcg ggcaccgtcc gcttcaagga tcgcgacatc
     4261 gccggcatcg ggcccgtgcg cctggcgcgg ctcggcatgg ctcggagctt ccagctcgtg
     4321 cacgtcttcc ccgagctcac cgtgctcgag acgctgcagg cggcggccgt gtcgcgcctc
     4381 ggccgcagcc gccgcctctt cgcgtcgctc gcgcgggacg ccgaggtccg ggacgccgcc
     4441 ctcgaggtgg cagagctgtt cggcctcgcc gacaagcgcc acgcgcgctc gaagcacctg
     4501 ccgcagggcg acaagaagct gctcgacgtc gcctcggcct tcgccctgcg cccggagatc
     4561 atcctgctcg acgagccgac cagcggcgtc agcacgcgcg acaagacggc gatcatggag
     4621 atcctcgtcg ccgccgcgaa gcggatcggg ctccaggcca tcatccaggt cgagcacgac
     4681 atggacatcg tcttcgccta ctccgaccgc atcgtggccc tccaccaggg ccgggtcctg
     4741 gccgacgcga cgccggccca gatccaggcc gaccagcgcg tggtggacac cgtgatcggc
     4801 cgccgcgcgg cgaccctctg agggttttgc gcgtgctgag cgtgacgggc gtcgacgtgt
     4861 tcatcgagac gagccagatc ttgcgggagg tctcgctcga agtcggcgag cgcgaggtcg
     4921 tgtgtctggt cgggcgcaac ggcgccggca agacgacggc gctccgcacg atcatggggt
     4981 atctgcggcc ccgggccggg cgcatcacgt tccgggaccg gcccatccac ggacggcgca
     5041 cgcacgagat cgcacggctc gggctcggct tcgcgcccga ggacggcgcg atcttcgggg
     5101 acctgacggt ggccgagaac atcgagatcg ccacgtggac gcggccgggc ggccgcccgg
     5161 cgccggagcg gctcgccctg gcctacgacg tcttccccaa cctcaggcag tacgctcgcc
     5221 gcaagggacc gcagatgagc ggcggcgagc gcaagatgct gtccatcgcg cgcgccctcg
     5281 cgctcgaccc cgagatgctg ctgctcgacg agcccttcga gggcctctcc ccggcgatca
     5341 tcccgacgat cgccgagggc ctcgcgacga tcacgagcct gggacacgcc atcctcctcg
     5401 cggagtcgaa catccaccac gtcccggtgt gggccacacg gctctacgtg atcgagcgcg
     5461 gcgagatcgt cttcgccggg acgcccgccg ccgcccgcag ggaccccgcc gtcgggcgca
     5521 tcatcggcgg aagcacgtga gcccctgtcc agcccgcgca tacgcgatga cgtttcaccc
     5581 tgtgggccgg gcgcgtgacc cgaattcgtc ctcttcccct gcgtgcgggc ggcgccgatc
     5641 gccggg
//
LOCUS       DATIAZ010000058         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1855730, whole genome shotgun sequence.
ACCESSION   DATIAZ010000058 DATIAZ010000000
VERSION     DATIAZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855730"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(839..1171)
                     /locus_tag="VLF19_01990"
     CDS             complement(839..1171)
                     /locus_tag="VLF19_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92049.1"
                     /translation="MRKHRHTQPSVPSKARHDNFNAANALESVYVDLIQVDAFAHAAR
                     EAIVELRCPSNRAERRTYNRIYTLVTKVADEARAALLHSDALVAALSAHMEARGTSAD
                     TDPSTPQT"
     gene            complement(1204..1452)
                     /locus_tag="VLF19_01995"
     CDS             complement(1204..1452)
                     /locus_tag="VLF19_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92050.1"
                     /translation="MVLPELAILAALQQLLELTTRTLTAVHPDAVVDHSRLRPLDLRA
                     TLADQIIELGACLARATTCYRVAAIDSLYAPDTDDIPF"
     gene            complement(1508..2350)
                     /gene="istB"
                     /locus_tag="VLF19_02000"
     CDS             complement(1508..2350)
                     /gene="istB"
                     /locus_tag="VLF19_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HSE92051.1"
                     /translation="MPKPARTQRRAKPEPRGDGGVLERLRDHLERLGLKELDQHLDAH
                     LAWAHDHAPSALVLLERVFGDGAQQLRQRRVERRIDTCGLRVRKTLEAFDWVFQKNLN
                     RAVIEGLATLGFIDDCEDLLITGKSGTGKSHILQALALRACEREIQVRYTRCVDLIDD
                     LYAGLADQTYEARLRRWARAEWLVIDDVGLGQLRRRDDEPTAAHMLFNLLDRRHEQAA
                     TAITSNIKLGEWGKYLGDATLAAAILDRLAMTSIRIDIDGPSYRDHLAKQRAKDRGIV
                     DAAA"
     gene            complement(2248..3909)
                     /gene="istA"
                     /locus_tag="VLF19_02005"
     CDS             complement(2248..3909)
                     /gene="istA"
                     /locus_tag="VLF19_02005"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSE92052.1"
                     /translation="MTRSREELAHDVVMLARQGMSVRAIARALHVGRNRVRKILAAHD
                     AARNGDAPPSALPPLPASRPSMLDAHEAFVRGLIARFPTITAQRVYEELQARQDPAFE
                     GSYTIVKKYVRALRPKPVVQISTPVEEPEPGKVAECDWATITIDFKNRTRQKLQVFGY
                     TLAYSHRRYYRLYDRADFHALLDGHVEAFDHLEGIAEECKYDGQKTVVLRWEGQQPIY
                     NPRFIAFATYYLFRPRACRPYHPNDKPHVELSFRALRISFFNGRDFHDLDDLKAQLAR
                     WMTGIDDERPQRKKQRRTPFELHAEEQPHLVARPRHAYDTARVVYRVCDLEGFVAWEG
                     NRYSLPVENVTELLPVRITQNEIFVYAQNLKLVARHELRRRGTGDDVVAPGHRPKPDR
                     GPDLDQLRLVYRELGDGADDFLLALERAQPRSAAYHARHILAFRERYRTDDVVAAMDH
                     ARQYGAFEYRAIERILVARAAPRRLDEYVAEATEKKLAAIVRQSRTEPRDLSAYDALP
                     NWSRPTHPGEPECPNQREPSAEPNPSPEETAASSNDSETTSSDSD"
     gene            complement(3899..>4580)
                     /locus_tag="VLF19_02010"
     CDS             complement(3899..>4580)
                     /locus_tag="VLF19_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92053.1"
                     /translation="AEIHEVTGPAAKLLLWQSNVRQELSDLEEAWLVRALYREDRLNQ
                     PQIAQLVGRHKSWVCRRLVLAEGLTSEVESDVRLGLISATAAREVGRLPRGNQEKVAQ
                     VIARRGLTTRQATRLVDQWLAATDEDARTTVLAEAERASAPPAVRGPRGVPVTPGEAI
                     IADAAALAFRAARLQARLLERPLASLGPEVERVVVGQLGELHAVVAALCRTLTHVGAM
                     NAEVSRDA"
BASE COUNT          656 a         1494 c         1653 g          777 t
ORIGIN      
        1 agatagggcc caaccatgag ccgcgccggc gctgtcgcga aacccctctg ccacgacttg
       61 aatatccatt caatcgccct taatccacgt tctggccgca gtgtggcaag tgcggtgcgg
      121 tgctggcacc gcaaaggcct accggaccgc cttcaggctt taccgtgatc gaccgcgaag
      181 cacctacgac ccggcgctac gtcaccgcgg aggtagcgcc atgacagcgc gtgctacggc
      241 gcgtgctgcc gccgtcgccg agtcataggc gccggctaga agtggatcca ttcgcggcgg
      301 tacggcggat ccagttcggt tgacgtgcgt tgcgttcgcc gaagcagagc cgcactcgct
      361 gggcagctgg gcagctgggc agctcggcag ctcggctgct gggctgggca gctcggcagc
      421 tcggcagctc ggcagctcgg cgtgtagcgt ttaggacggc ccccgttcga gttgtcgcgt
      481 tcacaggcca gcgctagcgg cgcggccggc acgacgactt cggtcacgag ccccggcgcc
      541 cacgtgggcg cggccatgcg acgatctcga tcaccgcccg gcgcccacgt gggcgcggcc
      601 catgtgacgg agctgaccgc gctcggatgg aagccggtca ttgcgcacgc tgcggtcgag
      661 gccgccgcgg cacggagctg gctctcgagc gatcgagctt cgagacgtta cggcgatgac
      721 cgcccgacgg taggagcgcg gtgtcatccg ccgaggctcg tcgtgatcgc gagtcaaggc
      781 cggtgaggcg gcgcctgggt tgccggcgcg ggttgttacg gctggatcgc gctcgacgtc
      841 aggtctgggg cgtcgacggg tcggtatctg cggacgtgcc gcgagcttcc atgtgcgctg
      901 agagtgcggc gaccagagcg tcactgtgga ggagcgcagc cctggcctcg tctgcgacct
      961 tcgtgaccaa cgtgtagatc cgattgtacg tgcgccgctc ggcgcgattt gacggacacc
     1021 gcaactcgac gatcgcctcc ctggcggcgt gtgcgaaggc atcgacctgg atcaggtcga
     1081 cgtagacgct ctcgagcgca tttgcggcgt tgaagttgtc gtgccgagcc ttgctcggta
     1141 cgctgggctg ggtgtggcgg tgtttgcgca tcgttgctcc ttggctcgtc ggcgtgtaac
     1201 cgctcagaag gggatgtcat ccgtgtcggg tgcgtagagc gagtcgatcg ccgcgacgcg
     1261 atagcaagtc gtcgccctcg cgagacacgc cccgagctcg atgatctggt cggcaagggt
     1321 cgcgcgtagg tccagtggac gcaggcggga gtggtcgacg acggcgtccg gatggacggc
     1381 ggtgagcgtg cgcgtcgtca gctcgagaag ctgttgcagg gcggcgagga tcgcgagctc
     1441 agggaggacc accagctcgt ccggcgtggg cgacgtcggt agcgtggcgc ctgccgctgc
     1501 ccgatgacta cgcggcggcg tcgacaatgc cacggtcctt ggcgcgctgc ttggccaggt
     1561 ggtcgcgata gctcgggccg tcgatgtcga tgcggatcga ggtcatggca agccggtcga
     1621 ggatcgcggc ggccagggtg gcgtcgccca ggtacttgcc ccactccccc agcttgatat
     1681 tcgaggtgat cgcggtggcg gcctgttcgt ggcgccggtc gaggaggttg aagagcatgt
     1741 gggccgcggt tggctcgtcg tcgcgacggc gtagctggcc gaggccaacg tcgtcgatca
     1801 cgagccactc ggcgcgcgcc cagcggcgga gccgggcttc gtaggtctga tccgcgaggc
     1861 cggcgtagag gtcgtcgatc aggtcgacac agcgagtgta gcggacctgg atctcgcgct
     1921 cgcaagcacg cagcgcgagc gcttgcagga tgtggctctt gcccgtgccg ctcttgccgg
     1981 tgatcaggag atcttcgcag tcgtcgatga agccgagggt cgccaggccc tcgatgacgg
     2041 cgcgattgag attcttctgg aacacccagt cgaaggcctc gagagtcttg cggacccgca
     2101 ggccacaggt gtcgatgcgt cgctcgacac ggcgctgccg gagttgctgg gcgccgtcgc
     2161 caaacacccg ctcgagcagc accagggccg acggagcgtg gtcgtgcgcc caggcgaggt
     2221 gtgcgtcgag gtgctggtcg agctctttca gtccgagtcg ctcgaggtgg tctcggagtc
     2281 gttcgaggac gccgccgtct cctcggggct cgggtttggc tcggcgctgg gttcgcgctg
     2341 gtttgggcat tcgggttctc ctggatgggt gggtctggac cagttgggaa gtgcgtcgta
     2401 ggcagagagg tcgcgcggct cggtccgact ctggcgcacg atggcggcga gcttcttctc
     2461 ggtcgcctcg gcgacgtact cgtcgagccg gcggggcgcg gcgcgggcaa cgaggatccg
     2521 ctcgatcgcg cggtactcga aggcgccgta ctggcgagcg tggtccatcg ccgcgaccac
     2581 gtcgtcggtc cggtagcgtt cgcggaaggc caggatgtgg cgcgcgtggt aggcggccga
     2641 gcgtggctgc gcgcgctcga gcgccagcaa gaagtcgtcg gcgccgtcac cgagctcgcg
     2701 gtacacgagg cgcagctgat cgaggtcggg gccacggtcg ggcttgggcc gatggcccgg
     2761 cgccacgaca tcgtcgccgg tgccgcgccg gcgcagctcg tgccgcgcga ccagcttgag
     2821 gttctgcgcg tacacgaaga tctcgttctg cgtgatccgg accggcaaga gctcggtgac
     2881 gttctcgacg ggtagcgagt agcggttgcc ctcccacgcg acgaagccct cgaggtcgca
     2941 cacccgatac accacccgcg cggtgtcgta ggcgtggcgc ggcctggcga cgagatgcgg
     3001 ctgctcctcg gcatgcagct cgaacggcgt ccggcgctgc ttcttgcgct gcggcctttc
     3061 gtcgtcgatg ccggtcatcc agcgcgcgag ctgcgccttg aggtcgtcga ggtcgtggaa
     3121 gtcacgcccg ttgaagaagc tgatccgcag cgcgcgaaag ctcagctcga cgtgcggctt
     3181 gtcattggga tgatacggac gacacgcccg tggccgaaat agatagtacg tcgcaaatgc
     3241 gatgaagcgt ggattataga tgggctgttg accttcccag cgcaacacga ccgtcttctg
     3301 cccgtcgtac ttgcactcct cggcgatgcc ctcgaggtgg tcgaaggcct cgacgtgacc
     3361 gtcgagcagc gcatgaaagt cggcacggtc gtacagccgg tagtaccgtc ggtggctata
     3421 cgcgagcgtg tagccgaaga cctgcagctt ttgccgcgtg cggttcttga agtcgatcgt
     3481 gatggtggcc cagtcgcact ccgcgacctt gccgggctcg ggctcctcga cgggcgtgga
     3541 gatctggacc accggcttgg gccgcagcgc gcggacatac ttcttgacga tcgtgtagct
     3601 gccctcgaag gcggggtcct ggcgagcctg gagctcctcg tagacccgct gcgccgtgat
     3661 ggtggggaag cgtgcgatca aacctcggac aaaggcctcg tgggcgtcga gcatcgacgg
     3721 tcgcgacgcc ggcagcggcg gcagcgcgct cggcggcgcg tcgccgttcc gcgcggcgtc
     3781 atgcgcggcg aggatcttcc ggacccggtt gcgcccgacg tggagtgcgc gggcgatcgc
     3841 gcgcacgctc atcccctgcc gggcgagcat cacgacgtcg tgggccagct cctcgcggct
     3901 acgcgtcacg ggacacctcc gcgttcattg ccccgacgtg ggtcagtgtc cggcacagcg
     3961 ccgcgacgac cgcgtggagc tctccgagct gcccgaccac cacccgctcg acctcgggcc
     4021 ccaggctcgc gagcgggcgc tcgagcagcc gagcctggag gcgcgctgcc cgaaacgcca
     4081 gcgccgcagc gtccgcgatg atggcctcgc cgggagtcac cggcacaccc cgcggtcccc
     4141 gcacggccgg aggcgcgctc gcacgctcgg cctcggcgag caccgtggtc cgggcgtcct
     4201 cgtcggtggc ggcgagccac tggtcgacca gccgcgtggc ctgccgcgtg gtcaagccac
     4261 gccgcgcgat cacctgagcg accttctcct ggttgccgcg cggcaaccgg ccgacttccc
     4321 gggccgccgt cgccgagatc agccccagtc ggacgtccga ctccacctcg ctggtgagcc
     4381 cctccgccag caccagccgg cgacacaccc agctcttgtg ccgtcccacg agctgggcga
     4441 tctgcggttg attgagccgg tcctcgcgat agagcgcgcg caccagccac gcctcctcga
     4501 gatccgacag ctcctgtcgc acgttgctct gccacagcag cagcttggcc gccgggcccg
     4561 tgacctcgtg gatctcggca
//
LOCUS       DATIAZ010000059         9851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1330192, whole genome shotgun sequence.
ACCESSION   DATIAZ010000059 DATIAZ010000000
VERSION     DATIAZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330192"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VLF19_02015"
     CDS             complement(<1..446)
                     /locus_tag="VLF19_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE92054.1"
                     /translation="MKVPALTEVSFRIGKGEFVVITGPSGAGKTTLLRLLYGDETPTE
                     GDIEILAQPVTELSRPKIQTLRRSLGVVFQDAKLLPGRTVYENIAFVLRVTGTPRREI
                     TARAFEALRAVGLSSRAQAYPAQLSQGEAQRASLARAIVRKQPVLIA"
     gene            complement(522..2027)
                     /gene="gatB"
                     /locus_tag="VLF19_02020"
     CDS             complement(522..2027)
                     /gene="gatB"
                     /locus_tag="VLF19_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSE92055.1"
                     /translation="MSAVAAETRYEVVIGLEVHAQLLTRSKMFCGCPTAFGGEPNTQT
                     CPVCQGMPGTLPVINRRAIEFGVKTALAFDCDVNRSCRFARKHYYYPDMPKNYQISQY
                     EEPLAGEGWLEIDLPGGATKRVGIQRLHLEEDVGKLLHEGTLETAQSSLVDYNRSGVP
                     LMETVSKPDIRSPEEAAAYLRAFRAVLIHLGVCDGNMEEGSLRCDANISLRARGQQEF
                     GTKVEIKNLNSFRNVQHALEYEVQRQARVLDAGERVVQETRLWDAERGATRSMRSKEY
                     AHDYRYFPEPDLVPLELDAAWVDEVRRGLPELPRARRHRYVETLGLPAYDAGVLTQSP
                     AITAYYEDAVRQLVPDAPPGSPRLARAAKATSNWVMSELMRVWSGDDEAAIGACRVTP
                     SHLAGLQRLIDEGTISGKIAKGVFEKMLATGEDARVIVDREGLTQLADSGELGAVIDR
                     VLADNPKPIEDFRKGKAQAQKALVGLVMKATAGKANPALVNQLLQEKLAHR"
     gene            complement(2024..3460)
                     /gene="gatA"
                     /locus_tag="VLF19_02025"
     CDS             complement(2024..3460)
                     /gene="gatA"
                     /locus_tag="VLF19_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSE92056.1"
                     /translation="MHELAQAYRRGDTTPTAVAEAYLARIEKLDGRVGAYLTVTAAQA
                     RQQAKAAEARLADQAALGPLDGVPVALKDVFCTRGVRTTCGSKILESFVPPYDATMVE
                     RLVRAGAVLLGKTNMDEFAMGSSTEHSAFKLSRNPWDLERVPGGSSGGSAAAVAGELA
                     AGAFGTDTGGSVRQPAAFCGVVGLKPTYGRVSRYGLIAFASSLDQVGPLARDVRDCAL
                     LLGAVAGVDPADSTSVDAPVPDYVAALEGGVTGLRLGVPREYFAEGLDPEVARAVREA
                     IAALERLGAVTEDISLPTTDYGIAVYYILAPAEASSNLARYDGVKYGLRVPGRDLIDM
                     ESHTRAAGFGTEVKRRIMLGTYALSAGYYDAYYGRAQKVRTLVKRDFARAFERVDLIV
                     APTTPNVAFRHGEKEDPLAMYLNDVYTVPTSLAGLPAVSIRCGFTTSGLPVGLQLIGK
                     AFDEATILRAAHAYERATPWLEHRPDLG"
     gene            complement(3588..3875)
                     /gene="gatC"
                     /locus_tag="VLF19_02030"
     CDS             complement(3588..3875)
                     /gene="gatC"
                     /locus_tag="VLF19_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSE92057.1"
                     /translation="MIDDEDVEHVARLARLALSAAEKARMREQLARILQYIEKLKELD
                     VEGVEPTSHAVPLVNVMRDDRIVPGLPRMAMLANAPDPVGEFVRVPRIIED"
     gene            complement(3872..6043)
                     /locus_tag="VLF19_02035"
     CDS             complement(3872..6043)
                     /locus_tag="VLF19_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSE92058.1"
                     /translation="MPADLERLLDDLNPAQCAAVTFGEGPLLVLAGAGSGKTRVIAYR
                     IAWLLGVRGVSPRNVLAVTFTNKAAGEMARRVDDLLLPAGLKSPLIATFHAACVRVLR
                     QNGEHIGLPKSFTIYDEDDRLALVKECMKEGELADRQFTPSAAVHRISYWKNQMTSVP
                     DALRDARGPWEQKAALVYSRYEKRLKETGAVDFDDLLLVVVRLFSEVPEVLAWYRGLW
                     RHVLVDEYQDTNRAQYRIIRLLTSEHRNVCVVGDSDQSIYKWRGADVRNILEFEDDYP
                     GTTVVKLEQNYRSTRRVLALASSVIANNVQRKDKTLWTENPEGDRARLYRAWDEHEEA
                     NFVAQTILGLRAEGIPWEGVAVFYRTNAQSRVLEDALRRARVPYVIVGGVRFYERKEI
                     KDALAHLRLTINATDDVAFRRAIGAISRGIGPSTVARLEEVARREDRSLLAVAAEPPV
                     DLKGKPRRTLEGFAATIARLAAQRRTMPPPQFIDLVLKDSGYRQALEQERTPEAEARL
                     ENLEELIAAAEEFTVGQQEATLEGFLDSVALMSDVDELKDEASRVTLMTLHSAKGLEF
                     PAVFMTGMEEGVFPHARSMNEQEEIEEERRLCYVGLTRARERLYLSWAVHRRVHGYGA
                     GEPSRFLREMPEDQILALNVRSGPPPEMLRDAVAHAAAAAASQDDLPLRVGARVCHSR
                     WGEGMVVGVEREDGDVLVTVRFASVGRKRLSLQYAHLEELE"
     gene            complement(6127..6450)
                     /locus_tag="VLF19_02040"
     CDS             complement(6127..6450)
                     /locus_tag="VLF19_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HSE92059.1"
                     /translation="MALGAFGAHLLRDRLGPPLMATFETGQRMHIFHTVALLAVAWAA
                     TRWPGAGVSAAGWLFVAGIVLFSGSLYLLAITGQRWLGAITPLGGLCFLGAWFLLARA
                     AWSGQ"
     gene            complement(6510..8351)
                     /gene="glmS"
                     /locus_tag="VLF19_02045"
     CDS             complement(6510..8351)
                     /gene="glmS"
                     /locus_tag="VLF19_02045"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSE92060.1"
                     /translation="MCGIVGYVGEKNAVGIIVDGLKRLEYRGYDSAGVAVLGAGGALE
                     VRRAAGRIKTLEGILRDRPLTGTVGIGHTRWATHGRPSDENAHPHTDCTGSLVVVHNG
                     ILENYLEIKERLRAEGHVFRSETDTEVIAHLVEHHLGQGARLDQAVKRAMGEFSGSYA
                     IGVVSSAAPDRLVAAKHGAGSVVIGLGKGEMFIASDIPAILSHTRDVVILEDGEVAEV
                     SADGVTLSTLDGEPVQREPVRILWDPIMAEKGGYRHFMLKEMYEQPRALTDTFRGRVS
                     IETGNVVLPDVNLDPSTVRSLQRVVFVACGTASYASLLGRMYVERLAGLPAEVDLGSE
                     FRYRDAPVGPETLVISVSQSGETADTLGAVKAARLKGCPILAITNVVGSALSREATGT
                     LYMHAGPEIGVASTKAFTAMIVAAYLVGLWLGRQREAITAEDVRKRIRDLVEIPRLVE
                     QTLELDGKIAALARYLSAADNFLYLGRGLQYPIALEGALKLKELSYIHAEGYAGGEMK
                     HGPIALIDDGFPVVALAPRDGSYERMLGNIEEVRAREGQVIAVVQSGDKLVAGKAQHV
                     IEVPSAADLLAPIVTVVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(8355..9743)
                     /gene="glmU"
                     /locus_tag="VLF19_02050"
     CDS             complement(8355..9743)
                     /gene="glmU"
                     /locus_tag="VLF19_02050"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HSE92061.1"
                     /translation="MDGLTVVILAAGEGKRMRSRQAKVLHALCGRPLLGYALRIARVL
                     ADRIVVVAPPNTDGVARLAGPDGATVVQHERLGTGHAVMQAREACEGAQTVLVLPGDM
                     PLLTVETIERLVTHHAATGAAVTVLTANVDTPHGYGRVLRQRGRVTRIVEERDATDDQ
                     KKVAEINTSVYCFDARRLWPALEEVRADNDQGEYYLTDILGILARAGARVEAVAVPDA
                     ADATGVNDRKQLASVAAIQRRRILDRLMESGVTILDPATTYIDDTVTIGPDTVVHPQA
                     VIEAATTIGSDCVIGAGCHVTGSRFADRVTLRPYSIIEDAVVEEDATLGPFCHLRPGC
                     HIGAAAEIGNFAELKKSRVGRKTKIHHVSYTGDATLGAHVNVGAGTITCNYDGQRKYQ
                     TVIGDGVFVGTNSSLVAPVTIGEGAYVAAGSVITKDVPPGALAVERSPQVVKDGWAAR
                     RAARRAAERKDG"
BASE COUNT         1376 a         3485 c         3489 g         1501 t
ORIGIN      
        1 gcgatcagca cgggctgctt gcggacgatg gcgcgcgcca gggacgcgcg ctgggcctcg
       61 ccctggctga gctgcgcggg gtaggcctgc gcgcgcgagg agagcccgac ggcgcggagc
      121 gcttcgaagg cgcgcgcggt aatctcgcgg cggggcgtcc cggtcacgcg cagcacgaac
      181 gcgatgttct cgtagacggt gcggcccggc aggagcttgg cgtcctggaa caccacgccg
      241 agggaacgtc ggagcgtctg gatcttcggg cgcgacagct cggtgaccgg ttgggccagg
      301 atctcgatgt caccctcggt cggcgtctcg tccccgtaca ggagccgcaa cagcgtcgtc
      361 ttgccggcgc cgctcgggcc ggtgatcacg acgaactcgc ccttgccgat tcggaaggag
      421 acctccgtga gggcagggac tttcaccggt ccgacctcgt acaccttcgt gacgcggtga
      481 aactcaatca tcgcgggcgc tctcgattat agcggcccgc ctcaccggtg agcgagtttc
      541 tcctggagga gctggttcac gagcgcgggg ttggccttgc cggccgtggc cttcatgacg
      601 agcccgacca gggccttctg cgcctgggcc ttgcccttcc tgaagtcctc gatcggcttg
      661 gggttgtcgg cgaggacgcg atcgatgacc gcgcccagct cgccgctgtc ggcgagctgg
      721 gtcagtccct cgcggtcgac gatgacccgc gcgtcctcgc ccgtggccag catcttctcg
      781 aacacgccct tggcgatctt gccgctgatc gtgccctcgt cgatgagccg ctggaggccg
      841 gcgagatgcg acggcgtcac gcggcacgcc ccgatggccg cctcgtcgtc gcccgaccac
      901 acgcgcatca gctccgacat cacccagttg gaggtggcct tggccgctct cgcgagcctc
      961 ggagagcccg gcggcgcgtc cgggaccagc tgcctcacgg catcctcgta gtacgccgtg
     1021 atggccgggc tctgggtcag cacgccggcg tcgtacgcgg gcagcccgag cgtctccacg
     1081 taccggtggc ggcgcgcgcg gggcagctcg ggcagaccgc gccgaacctc gtccacccag
     1141 gcggcgtcga gctcgagcgg gaccaggtcg ggctccggga agtagcggta gtcgtgcgcg
     1201 tactccttcg agcgcatcga gcgggtggcg ccgcgctcgg cgtcccagag ccgcgtctcc
     1261 tgcacgacgc gctcgccggc gtccagcacc ctggcttgcc gctgcacctc gtactccagc
     1321 gcatgctgga cgttgcggaa cgaattcagg ttcttgatct cgaccttagt gccgaactcc
     1381 tgctgccccc gcgcacgcag ggagatgttg gcgtcgcagc gcagcgagcc ctcctccatg
     1441 ttgccgtcgc agacgccgag gtggatcagc accgcgcgga aggcgcggag ataggcggcg
     1501 gcctcttccg gtgaccggat gtcgggcttc gacaccgtct ccatcagcgg cacgcccgag
     1561 cggttgtagt cgacgaggct cgactgcgcg gtctcgagcg tgccctcgtg tagtagcttg
     1621 ccgacgtcct cctccagatg cagccgctgg atgcccacgc gtttcgtcgc cccgccgggc
     1681 aggtcgatct ccagccagcc ctcgccggcg agtggctcct cgtactggct gatctggtag
     1741 ttcttcggca tgtcagggta gtagtagtgc ttgcgcgcga accgacacga acgattgacg
     1801 tcgcagtcga aagcgagcgc cgtcttcacg ccgaactcga tggcgcgccg gttgatcacg
     1861 ggcagcgtcc ccggcatgcc ctggcagacc gggcaggtct gcgtgttggg ctcgccaccg
     1921 aatgccgtgg ggcagccgca gaacatcttc gagcgtgtca ggagctgggc gtgcacctcg
     1981 aggccgatga cgacctcgta gcgggtctcc gcggccacgg cgctcatccg agatcgggcc
     2041 ggtgctcgag ccagggggtg gcccgctcgt aggcgtgggc cgcgcgcagg atcgtggcct
     2101 cgtcgaaggc cttgccgatg agctggagtc cgacgggtag cccggacgtg gtgaagccgc
     2161 agcggatcga gaccgccggc agcccggcca ggctcgtcgg caccgtgtac acgtcgttga
     2221 gatacatcgc cagtgggtcc tccttctcgc cgtgccggaa cgccacgttc ggcgtcgtcg
     2281 gcgccacgat caggtcgacc cgctcgaacg cccgcgcgaa gtcgcgcttg accagcgtgc
     2341 gcaccttctg cgctcgcccg tagtaggcgt cgtagtagcc cgccgagagg gcgtaggtgc
     2401 cgagcatgat ccgccgcttg acctcggtgc cgaagcccgc cgcgcgggtg tggctctcca
     2461 tgtcgatcag gtcgcggccg ggcacgcgca ggccgtactt cacaccgtcg tagcgcgcca
     2521 ggttcgacga ggcctcggcg ggggcgagga tgtagtagac ggcgatgccg tagtccgtgg
     2581 tgggcagcga gatgtcctcg gtcaccgcgc cgagccgctc cagcgcggcg atcgcctcgc
     2641 gcacggcgcg ggcaacctcg gggtcgaggc cctcggcgaa gtactctcgc ggcaccccga
     2701 ggcgcaagcc cgtcacgccg ccctcgagcg ccgcgacgta gtctgggacg ggtgcgtcga
     2761 cggacgtcga gtcggcggga tcgacgccgg ccacggcgcc gagcaggagc gcacagtcgc
     2821 ggacgtcccg ggcgagcggc ccgacctggt cgagcgacga ggcgaaggcg atcaggccgt
     2881 agcgcgatac ccgcccgtag gtcggcttga ggcccaccac gccgcagaag gccgccggct
     2941 gccgcacgga gccgccggtg tcggtgccga aggcgccggc ggcgagctcg ccggccacgg
     3001 cggccgcgga gccccccgag gagccgccgg gcacccgctc gagatcccac gggttgcgcg
     3061 agagcttgaa ggcggagtgc tcggtggacg agcccatcgc gaactcgtcc atgttggtct
     3121 tgccgagcag cacggccccg gcccgaacga gccgctcgac catggtggcg tcgtagggcg
     3181 gcacgaacga ctcgaggatc ttcgagccgc aggtcgtgcg cactccccgc gtgcagaaca
     3241 cgtccttgag cgcgaccggc acgccgtcga gcgggccgag tgccgcctgg tcggcgaggc
     3301 gggcctcggc ggccttggcc tgttgccggg cctgcgcggc cgtcaccgtg aggtacgcgc
     3361 cgacacgccc gtcgagcttc tcgatgcgcg cgaggtacgc ctccgcgacc gccgtgggcg
     3421 tggtgtcgcc ccggcggtag gcctgcgcca gctcgtgcat ggcgagccgt gtcagctcgc
     3481 tcacggccat cccgccgcgg ccgcgatccc cacgctccgt tccatccccg gtcgtctccg
     3541 ccagcatcct cggtctgcgc ccgaagcccc ggtcctcgcc gacgctctca gtcttcgatg
     3601 atccgcggca cgcggacgaa ctcgccgacc gggtcgggcg cgtttgccag catggccatc
     3661 ctcgggagac ccggcacgat cctgtcgtcg cgcatcacgt tgacgagggg caccgcatgc
     3721 gacgtcggct cgacgccctc gacgtcgagc tccttcagct tctcgatgta ctggaggatc
     3781 ctggccagct gctcgcgcat gcgcgccttc tcggcagcgg acagcgccag ccgggcgagc
     3841 cgggcgacgt gctcgacgtc ctcgtcgtcg atcattccag ctcctccaga tgcgcgtact
     3901 ggagcgacag ccgcttgcgt ccgaccgagg cgaaccggac ggtgacgagg acgtcgccgt
     3961 cctcccgctc gacgccgacc accatcccct cgccccagcg cgagtgacag accctggcgc
     4021 cgaccctgag gggcaggtcg tcctggctcg ccgcggcggc ggcggcgtgg gcgacggcgt
     4081 cgcgcagcat ctccgggggc ggtcccgacc tgacgttgag ggcgaggatc tggtcttccg
     4141 gcatctcgcg caggaaccgc gagggctcgc ccgcgccgta gccgtgcacc cggcggtgca
     4201 ccgcccagga gaggtagagc cgctcgcgcg cccgcgtgag gccgacatag cagagccgcc
     4261 gctcctcctc gatctcctcc tgctcgttca tcgaccgcgc gtgcgggaag acgccctcct
     4321 ccatccccgt catgaagacg gcggggaact cgaggccctt ggccgagtgc agcgtcatga
     4381 gggtcacccg ggaggcctcg tccttcagct cgtccacgtc gctcatgagc gcgacgctgt
     4441 cgaggaagcc ctcgagcgtc gcttcctgct ggcccacggt gaactcctcg gcggcggcga
     4501 tcagctcctc gaggttttcg agccgcgcct cggcctcggg cgtgcgctcc tgctcgagcg
     4561 cctgccggta gccggagtcc ttgagcacga ggtcgatgaa ctggggcggc ggcatcgtgc
     4621 ggcgctgggc ggccagccgc gcgatcgtcg ccgcgaagcc ctccagcgtc ctgcgcggct
     4681 tgcccttgag atcgacgggc ggctcggcgg ccaccgccag cagcgaccgg tcctcgcggc
     4741 gggcgacctc ctccaggcgc gccaccgtgg acgggccgat gccgcgggag atcgcgccga
     4801 tcgcgcggcg gaaggccacg tcgtcggtcg cgttgatcgt gaggcgcagg tgcgcgagcg
     4861 cgtccttgat ctccttgcgc tcgtagaacc gcacgccccc cacgatgacg tacgggacgc
     4921 gcgcacgcct gagcgcatct tccagcaccc gggactgggc gttggtacgg tagaagaccg
     4981 cgacgccctc ccaggggatg ccctcggcgc gcagaccgag gatcgtctgg gcgacgaagt
     5041 tcgcctcctc gtgctcgtcc caggcgcgat agagccgggc cctgtcgccc tccgggttct
     5101 ccgtccagag tgtcttgtcc ttgcgctgga cgttgttggc gatgacggac gaggcgagcg
     5161 cgaggacccg ccgggtcgac cggtagttct gctccagttt gacgaccgtg gtcccgggat
     5221 agtcatcctc gaattcgagg atgttgcgga cgtccgcccc ccgccacttg tagatcgact
     5281 gatcactgtc tccgacgaca cacacgttcc ggtgctccga cgtcagcagc cggatgatcc
     5341 ggtactgggc gcggttcgtg tcctggtact cgtcgaccag cacgtggcgc cagaggccgc
     5401 gataccacgc gagaacctcg ggaacctccg agaagagacg cacgaccacc agcagcaggt
     5461 cgtcgaagtc gaccgcgccc gtctccttga gccgcttctc gtagcgggag tagacgagcg
     5521 ccgccttctg ctcccacggc ccgcgcgcgt cgcgcagcgc atccggcacg ctcgtcatct
     5581 ggttcttcca gtacgagatg cggtggacgg cggcgctcgg cgtgaactgc cggtcggcca
     5641 gctcgccctc cttcatgcac tccttgacga gggccaggcg gtcgtcctcg tcgtagatcg
     5701 tgaagctctt cggcaggccg atgtgctcgc cgttctggcg aaggacgcgc acgcacgccg
     5761 cgtggaaggt ggcgatcagc ggcgacttga gccccgcggg taacagcagg tcgtccaccc
     5821 gccgcgccat ctcgccggcc gccttgttcg tgaaggtgac ggcgagcacg ttgcgcggcg
     5881 agacgcccct gacgccgagg agccacgcga tccggtaggc gatgacgcgc gtcttgccgc
     5941 tgcccgcgcc cgccagcacc agcagcggtc cttctccgaa ggtgaccgcc gcgcactgcg
     6001 cgggattcag gtcgtcgagg aggcgttcca ggtcagccgg caccgaccga ttgtaccgta
     6061 ccggtcaccg gatgccgacg acccggccgg tgccgacgcg ccggggcgcg gctcaggcgc
     6121 gggctctcac tgccccgacc acgccgcccg cgccagcagg aaccacgcgc ccaggaagca
     6181 gagcccgccc agcggcgtga tcgcgccgag ccagcgctgg ccggtgatcg ccagcagata
     6241 gaggctcccg gagaagagca cgatgccggc cacgaagagc cagccggccg cactcacccc
     6301 ggcgcccggc cagcgcgtcg ccgcccaggc gacggcgagc aacgcgacgg tgtggaagat
     6361 gtgcatccgc tggcccgtct cgaaggtcgc catgagcggc ggcccgaggc ggtcgcgcag
     6421 caggtgggcc ccgaaggcgc cgagcgccac ggcgaggaag cccgagaccg accccagaac
     6481 gaggaagagc cggtcgggag ccgtcgaccc tactccaccg tcaccgactt cgcgaggttg
     6541 cggggctggt cgacatcgca gccgcggcgc acggcgatgt ggtaggcgag gagctgcagc
     6601 ggcaccacgg tgacgatcgg cgccaggagg tcggcggccg acggtacctc gatgacgtgc
     6661 tgggccttgc cggcgaccag cttgtcgccg ctctggacca cggcgatcac ctggccctcg
     6721 cgcgcgcgga cctcctcgat gttgccgagc atgcgctcgt aggacccgtc gcgcggcgcc
     6781 agcgccacca ccgggaagcc gtcgtcgatg agtgcgatcg gaccgtgctt catctcgccg
     6841 cccgcgtagc cctcggcgtg gatgtaggac agctccttga gcttgagcgc gccctccagc
     6901 gcgatgggat actgcagccc ccgcccgagg tagaggaagt tgtcggcggc ggacaggtag
     6961 cgcgccagcg cggcgatctt gccgtcgagt tcgagcgtct gctcgaccag ccgggggatc
     7021 tcgaccaggt cccggatgcg cttgcggacg tcttccgcgg tgatcgcctc gcgctgacgg
     7081 ccgagccaga ggccgaccag gtacgcggcc acgatcatcg cggtgaaggc cttggtggag
     7141 gccacgccga tctcgggccc ggcgtgcatg tagagcgtgc ccgtggcctc gcgcgacagc
     7201 gccgagccga cgacgttggt gatggcgagg atcgggcacc ccttgagccg cgccgccttg
     7261 accgccccca gcgtgtcggc cgtctcgccc gactgtgaga ccgagatcac cagggtctcg
     7321 ggccccaccg gcgcgtcccg gtagcggaac tccgagccga ggtcgacctc ggccggcagt
     7381 ccggccagcc gctcgacgta catccggccc agcagcgagg cgtacgaggc ggtgccgcag
     7441 gcgacgaaga cgacccgctg cagactgcgg acggtggaag gatcgaggtt gacgtcgggc
     7501 agcaccacgt tgccggtctc gatcgagacg cggccgcgga aggtgtcggt cagggcgcgc
     7561 ggctgctcgt acatctcctt gagcatgaag tgccgatagc cacccttctc ggccatgatc
     7621 ggatcccaga ggatgcgcac cggctcgcgc tgcacgggct caccgtcgag cgtcgagagc
     7681 gtcacgccgt cggcgctgac ctcggccacc tccccgtcct cgaggatcac cacgtcgcgc
     7741 gtgtgcgaga ggatcgccgg gatgtccgag gcgatgaaca tctcgccctt gccgaggccg
     7801 atcaccacgc tgccggcgcc gtgcttggcg gcgaccagcc ggtcgggcgc ggcgctggag
     7861 accacgccga tggcgtagga gccgctgaac tcgcccatcg cccgcttgac cgcctggtcg
     7921 agccgcgcgc cttgcccgag gtggtgctcg accaggtggg cgatcacctc ggtgtccgtc
     7981 tctgacctga aaacgtggcc ctcggcgcgg aggcgctcct tgatctcgag gtagttctcg
     8041 aggatgccgt tgtggacgac gacgagcgag cccgtgcagt cggtgtgcgg gtgtgcgttc
     8101 tcgtcggatg ggcggccgtg cgtggcccag cgcgtgtggc cgatgccgac ggtaccggtc
     8161 agcgggcggt cgcggaggat gccctcgagg gtcttgatcc gccccgccgc ccggcgcacc
     8221 tcgagcgcgc cgccggcgcc gagcacggcc acgcccgcgg agtcgtaccc gcgatactcc
     8281 aggcgcttga ggccgtccac gatgatcccc accgcgttct tctcaccgac gtagccgacg
     8341 atcccgcaca tcggtcagcc gtccttccgc tcggcggccc gacgcgccgc gcgccgtgcc
     8401 gcccagccgt ccttcaccac ctgcggcgat cgctcgacgg cgagcgcgcc cggcggcacg
     8461 tccttggtga tcacggagcc ggcagccacg taggcgccct ccccgatcgt gacgggcgcc
     8521 acgaggctcg agttcgtgcc cacgaaaacg ccgtcgccga tgaccgtctg gtacttgcgc
     8581 tgcccgtcgt agttgcaggt gatggtgccg gcaccgacgt tgacgtgggc gcccagcgtg
     8641 gcgtcgccgg tgtaggagac gtggtggatc ttggtcttgc gaccgacccg cgatttcttc
     8701 agctcggcga agttgccgat ctccgccgcg gcgccgatgt ggcagcccgg ccgcaggtgg
     8761 cagaacgggc cgagggtggc gtcctcctcc accacggcgt cctcgatgat gctgtacggc
     8821 ctgagcgtga cccggtcggc gaagcggctt ccggtcacgt ggcagccggc gccgatcacg
     8881 cagtcgctcc cgatcgtggt cgcggcctcg atcacggcct gggggtgcac gacggtgtcg
     8941 ggaccgatgg tcaccgtgtc gtcgatgtag gtcgtggccg ggtcgaggat cgtgaccccg
     9001 gactccatca agcggtccag gatgcgccgc cgctggatcg cggcgacgga cgcgagttgc
     9061 ttgcggtcgt tcacgcccgt ggcgtcggcg gcgtcgggca cggccacggc ctcgacgcgc
     9121 gcgccggcgc gcgccaggat gccgaggatg tcggtcaggt agtactcgcc ctggtcgttg
     9181 tccgcgcgca cctcctcgag cgccggccac aggcgcctcg cgtcgaagca gtagaccgag
     9241 gtgttgatct cggcgacctt cttctggtcg tcggtggcgt cgcgctcttc cacgatgcgt
     9301 gtcacgcgcc cgcgctggcg cagcacgcgc ccgtacccgt gcggggtgtc gacgttggcc
     9361 gtgagcacgg tcaccgccgc gcccgtggcc gcgtggtgcg tgacgaggcg ctcgatggtc
     9421 tcgacggtga gcagcggcat gtcgccgggc aggacgagga cggtctgcgc gccctcgcac
     9481 gcctcgcgcg cctgcatgac tgcgtggccg gtgcccagcc gctcgtgctg caccacggtg
     9541 gccccgtcgg gtccggcgag cctggccacc ccatccgtgt tgggcggcgc gacgaccacg
     9601 atgcggtcgg cgaggactcg ggcgatgcgg agggcgtagc caaggagcgg gcgaccgcag
     9661 agcgcgtgca gcaccttggc ctggcgggag cgcatccgct tgccttcgcc cgctgcgagg
     9721 atgacgacgg tcaggccgtc catgcagtgt ctctcatcat agcatcggga attcggcaag
     9781 ccccgcgcca tgccggggcg cctggaatca ctcgaaaaac tggcggaatc agccagcggt
     9841 gtggcggaga t
//
LOCUS       DATIAZ010000060         2059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1593618, whole genome shotgun sequence.
ACCESSION   DATIAZ010000060 DATIAZ010000000
VERSION     DATIAZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593618"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VLF19_02055"
     CDS             <1..612
                     /locus_tag="VLF19_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSE92062.1"
                     /translation="LALVVSGGHTALYHAKAPLQYALVGQTRDDAAGEAFDKVAKLLG
                     LGFPGGPAIQRAAGSGDPRAIPFPLAQMTDQSRDFSFSGIKTSVSLHVKRHAPLSGAQ
                     VADVAASFQAAVVKMLVRRTIRAALHLGVKRVVVTGGVASNGPLRQALREEADAHGIR
                     LYIPPPRLCTDNAAMITAAGTARLAAGERADLSMNAIPDLALA"
     gene            710..1783
                     /locus_tag="VLF19_02060"
     CDS             710..1783
                     /locus_tag="VLF19_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92063.1"
                     /translation="MNYEAVLAGLPEAVIAVDAALRVVFWNAAAEELVGRSARRAQGR
                     LVKEVLSPGSSLVSRLAETLATGESRSEADAALDTADGRTVPASLVTAPLFARDGTVE
                     GAVAVLRDVSRLRQLEAEVRRGETLAAAGRVAVGVAHEIRNPLGAIRGAVQLLQRELG
                     DEPRLAEYTAVLLKEVDRVNRIIETLLDLGRPVQLQRVPLNLHQLLERVTLLHAEAAA
                     PRGVTFVRRYDPSLPPILGDEDRLLQVFHNLVVNALDAMPRGGQLTLASRVSRDPLYG
                     KMDTGGGQRTMVEVQVIDEGAGIPAAARARIFDLFFTTKDRGLGLGLALCHRIIEEHR
                     GAIHVESTEGRGTTVTCFLPVAR"
     gene            1786..>2059
                     /locus_tag="VLF19_02065"
     CDS             1786..>2059
                     /locus_tag="VLF19_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92064.1"
                     /translation="MARILIADDEDSLRWVLEKGLRGAGYEVMPVEDGEAALRAIERE
                     PFDLVLLDVRMPGLDGLTVLERVRELRPDTYVVVMTAHGTMETAIKA"
BASE COUNT          274 a          742 c          763 g          280 t
ORIGIN      
        1 ctcgcgctcg tcgtgtcggg cggccacacc gcgctgtatc acgcgaaggc gccgctgcag
       61 tacgcgctgg tcgggcagac gcgggacgac gccgccggcg aggcgttcga caaggtcgcg
      121 aagctgctgg gactcggctt cccgggcggg ccggccatcc agcgggccgc cgggtccggc
      181 gacccgcgcg cgatcccgtt cccgctggca cagatgacgg accagtcgcg cgacttctcg
      241 ttcagcggca tcaagacgtc ggtgtccctg cacgtcaagc gccacgcgcc cctctccggc
      301 gcccaggtgg cggacgtcgc ggcctcgttc caggccgcgg tggtcaagat gctggtgcgc
      361 aggacgatcc gggcggcgct tcacctggga gtcaagcggg tggtggtcac gggcggcgtc
      421 gcctcgaacg gtccgctgcg gcaggcgctg cgggaggagg cggacgccca cggcatccga
      481 ctgtatattc caccgccgcg gctctgcacc gacaacgccg cgatgatcac ggcggccggc
      541 accgcgcggc tggcggccgg cgagcgcgca gacctctcga tgaacgcgat ccccgatctg
      601 gccctggcat gacgcccgcg cgccacgcgc ggctcgccgc ttcgagcgcc ggcattgccg
      661 gtgcccgggg ggaggcctcg ggagcggctg ccggggccac ctccgactca tgaactacga
      721 ggcagtgctg gccggtctgc ccgaggccgt gatcgccgtc gacgcggcgc tgcgcgtggt
      781 gttctggaac gcggcagcgg aagagctggt gggccgctcg gcccggcggg cgcagggccg
      841 gctcgtgaag gaggtcctgt cgccggggtc gtcgctggtc agccggctcg ccgagactct
      901 cgccaccgga gagagccgct cggaagccga cgccgccctg gacaccgcgg acggtcgcac
      961 ggttccggcc agcctggtca cggcaccgct cttcgcgcgc gacggcaccg tcgagggggc
     1021 ggtggccgtg ctgcgcgacg tctcgcgcct tcgccagctc gaggccgagg tccgccgcgg
     1081 cgagacgctg gccgcggcgg ggcgcgtggc cgtgggcgtc gcgcacgaga tccgcaaccc
     1141 gctcggcgcc atccgcggcg cggtgcagct cttgcaacgc gagctgggcg acgagccgcg
     1201 gctcgcggag tacacggccg tcctcctcaa ggaggtcgac cgcgtcaatc ggatcatcga
     1261 gaccctgctc gacctcggac ggcccgtgca gctccagcgc gtgccgctca acctgcacca
     1321 gctcctcgag cgcgtgacgc tgctgcacgc ggaggcggcg gcgccgcgtg gcgtgacctt
     1381 cgtccggcgc tacgacccga gcctgccccc gatcctgggt gacgaggacc gcctgctgca
     1441 ggtctttcac aacctcgtcg tgaacgccct cgacgcgatg ccgcgcggcg gccagctcac
     1501 gctggcctct cgcgtgagcc gggatcccct ctacggcaag atggacaccg gcggaggcca
     1561 gcgcacgatg gtggaggtgc aggtgatcga cgagggcgcc ggtatcccgg cggccgcgcg
     1621 cgcccgcatc ttcgacctct tcttcaccac caaggatcgc gggctcgggc tcggcctggc
     1681 gctgtgccac cggatcatcg aggagcaccg gggcgccatc cacgtcgagt ccacggaagg
     1741 tcgcggcacc acggtgacct gcttcctgcc ggtcgctcga tgaccatggc gcggatcctg
     1801 atcgcggacg acgaggacag cctgcgctgg gtcttggaga agggcctgcg cggcgcgggc
     1861 tacgaggtga tgcccgtcga ggacggcgag gcggccctgc gcgccatcga gcgcgagccg
     1921 ttcgacctcg tgctgctcga cgtgaggatg ccgggcctcg acgggctcac cgtgctcgag
     1981 cgcgtgcgcg agctgcggcc ggacacctac gtcgtcgtga tgaccgcgca cggcacgatg
     2041 gagacggcca tcaaggcca
//
LOCUS       DATIAZ010000061         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_409084, whole genome shotgun sequence.
ACCESSION   DATIAZ010000061 DATIAZ010000000
VERSION     DATIAZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409084"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VLF19_02070"
     CDS             complement(<1..421)
                     /locus_tag="VLF19_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSE92065.1"
                     /translation="MNVEAPGGAPVAATELAARIFADRNAAFLRLAAGVAHELRNPLA
                     VILARAQLLALEIAQGRRPEPVKIESVLKIIEEQAIRASGIIESLSMFARPRRPVLDA
                     LDMATVVQDALQTLRQRAEAAGVTVEVAIEQAPVTLSA"
     gene            complement(581..>2012)
                     /locus_tag="VLF19_02075"
     CDS             complement(581..>2012)
                     /locus_tag="VLF19_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSE92066.1"
                     /translation="GWTVTDATQTDFEAEMEAQRERARASASFRAAEGDGEGTGVYQR
                     LSAEIPPVEFVGYETLTSPARVLALVTTRDGLTRVREAGAGDEVEVILDRTPAYAESG
                     GQVGDTGTLVGRDGRGEILDTYYRGAKLIVHRVRVRQGGFREGEDVAVSVESPRRLGL
                     RQHHTGTHLLHAALRRVLGTHVAQAGSLVAPDHLRFDFSHGAALEDRQVRRIEELVNE
                     QAQANVAVSRMEMDLDEALRTGALALFGEKYGQRVRVIKIGDFSTELCGGTHLDQTGQ
                     LGLLKISAEGAVASGVRRVEAVAGPAALAAVARQERTLREAAEILKIAPGEVPQRLRK
                     LLEEQRALEKQLQELEARLARSRADDLVAGATQVNGIAVVAERLDGLDADGLRAVVDR
                     VRERLGSGVVVVGGAAGGKVALVSGVTRDLTPRIHAGKLMQEVARAVGGTGGGRPDLA
                     QGGAKDDSRLSELKARVLAYVGGRGQ"
BASE COUNT          239 a          779 c          724 g          270 t
ORIGIN      
        1 cggcgctgag cgtcaccggc gcctgctcga tcgcgacctc gaccgtgacc cccgcggcct
       61 cggcgcgctg gcggagcgtc tggagcgcgt cctgcacgac cgtggccatg tcgagcgcgt
      121 cgagcacggg ccggcgcggc cgcgcgaaca tcgacaggct ctcgatgatc cccgaggcgc
      181 ggatcgcctg ctcctcgatg atcttcagca cgctctcgat cttgaccggc tcggggcgac
      241 gaccctgcgc gatctcgagc gccaggagct gggcccgggc gaggatcacc gcgagcgggt
      301 tgcgcagctc gtgcgccacg ccggccgcca gtcggaggaa cgccgcgttc ctgtcggcga
      361 aaatccgggc agccagttcg gtcgcggcca cgggcgcgcc gccgggggcc tcgacgttca
      421 tgccgagagc tattctagcc ggaccgccgg gccggcccct aagagcccgt cgggtgccgt
      481 attgccacgg cctggccggg aggcgctggg gtgtcggggc cgggtccggg ccccgtgctc
      541 tgggaaccgg tcgggactcg gaacatcgag gtggccacgg tcattgaccg cggccgccga
      601 cgtaggccag caccctggcc ttcagctcgg acagccggct gtcgtccttc gccccaccct
      661 gcgccaggtc gggccgcccg ccgcccgtcc cccctaccgc cctggccacc tcctgcatga
      721 gcttgccggc gtggatgcgg ggtgtcaggt ccctggtgac cccgctcacg agcgcaacct
      781 tgccgccagc cgcgccgccg acgaccacga cgccggagcc gagccgctcg cggacacggt
      841 ccaccaccgc gcgcaggccg tcggcgtcca gcccgtccag ccgctccgca accacggcga
      901 tgccgttgac ctgcgtggcg ccggccacga ggtcgtcggc gcgcgagcgc gccagcctcg
      961 cctccagctc ctggagctgc ttctccagcg cgcgctgctc ctcgagcagc ttgcgcaggc
     1021 gctggggcac ctcgcccggc gcgatcttga ggatctcggc ggcctcgcgg agggtgcgct
     1081 cctgccgggc gacggcggcg agcgccgccg ggccggcgac ggcctcgacc cggcggacgc
     1141 ccgaggccac ggcgccctcc gcggagatct tgagcagccc gagctggccc gtctggtcca
     1201 ggtgcgtgcc gccgcagagc tcggtggaga agtcgccgat cttgatcacg cgcacgcgct
     1261 ggccgtactt ctcgccgaag agcgcgagcg cgcccgtccg gagcgcctcg tcgaggtcca
     1321 tctccatccg gctcacggcc acgttggcct gtgcctgctc gttgaccagc tcctcgatcc
     1381 gccggacctg acggtcctcg agcgccgcgc cgtgggagaa gtcgaagcgc aggtggtcgg
     1441 gcgccacgag cgagcccgcc tgcgccacgt gggtgccgag cacccggcgg agcgcggcgt
     1501 gcagcaggtg cgtgcccgtg tggtgctggc gcagccccag gcgccgcggc gactcgacgc
     1561 tgacggcgac gtcctcgccc tcgcggaacc cgccctggcg cacgcgcacg cggtggacga
     1621 tcagcttggc gccgcgatag taggtgtcga ggatctcgcc gcggccgtcc cggccgacga
     1681 gggtgcccgt gtcgcccacc tgaccgccgg actccgcgta ggccggggtg cgatcgagga
     1741 tcacctcgac ctcgtcgccc gcgccggcct cgcgcacgcg cgtgaggccg tcgcgggtcg
     1801 tcaccagcgc gagcacgcgg gccggcgacg tgagcgtctc gtagccgacg aactcgaccg
     1861 gcgggatctc cgccgacagg cgctgataga cgccggtgcc ctcgccgtcg ccctcggcgg
     1921 cgcggaagga cgcgctcgcg cgcgcccgct cgcgctgcgc ctccatctcg gcctcgaagt
     1981 ccgtctgcgt cgcgtcggtg acggtccagc cc
//
LOCUS       DATIAZ010000062         6100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_541669, whole genome shotgun sequence.
ACCESSION   DATIAZ010000062 DATIAZ010000000
VERSION     DATIAZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541669"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VLF19_02080"
     CDS             <1..346
                     /locus_tag="VLF19_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92067.1"
                     /translation="RYMASSWTNPARSWSWTRRRGNLVVRASFSALEDVDPVSQVFRE
                     RLRIEGIENGTHRVYRQTHLSRMVFPQELRALVELAGDFEFVQWFFGFKPHQVLERSR
                     HPLLMVVVLRKT"
     gene            539..1702
                     /locus_tag="VLF19_02085"
     CDS             539..1702
                     /locus_tag="VLF19_02085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HSE92068.1"
                     /translation="MAKAILRLKRERERRALTHPWIFKGDVADVTDAPPDAVVTVVDA
                     AGRFVGRGFFNPRPALCCRIVTWEDEPLDDHLLARRVAAAVARRGATATLGRLVWSEA
                     DGLPGLVADRYGPVLVLQCLTRAMASHRPALVEALRAATADLPIFSADEATPAGLEGF
                     EPVRGWVDREGPARVIVEETWVRLAVTLGGGHKTGLYLDQRENRPRVGALATGRDVLD
                     AFAYTAAFACHALAAGARRALCLESSPEAIAGARENLALNGLADRAEIVAGNAFDELR
                     RLERTRARFGLIVLDPPPFARGRTTLDAAARGYKEINLRAMRLLDRGGALATFSCSHH
                     VSGGFFEEICRAAATDAGVRLRIVATLGQPADHPIVLTVPETGYLKGLLLQAW"
     gene            1711..2607
                     /locus_tag="VLF19_02090"
     CDS             1711..2607
                     /locus_tag="VLF19_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92069.1"
                     /translation="MTAPGLPPIDGTWYCDLWDPDLKILHRVRTTLFDGASAFQTIEV
                     ADTCGFGRMLILDGLPQAAEKDEMVYAKALAWPALLAVPGPKRVLVTGGGDGHVMREV
                     LRFPSVTSVVVCDIDSMVTKVTQEHMPFMWDGAERDRRAEIRHADAWEFLGSAAPGSF
                     EVVISDITDPAGEGSASHHLYSPAYFQRIRRCLAPGGVCAAQAQELSVRDFAHHARLR
                     RCVASVFAGVRSGHVYMPSFGYPEGLLFASDDPARLGLGRVEVEARLREAGLAADAHF
                     DAAVHAAMFTLPPLIRAALEKS"
     gene            complement(2619..3659)
                     /locus_tag="VLF19_02095"
     CDS             complement(2619..3659)
                     /locus_tag="VLF19_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE92070.1"
                     /translation="MARSTSALVLAVLVCAGCAGVGGPVPGAPPHHRERGFANPNPAF
                     VHPTFWAIQKFRLSRLWRGISANRPVPDLPRTANDGAALRANSGRPTVTWVGHATLLV
                     QLDGVNVLTDPIWSERAGPTSWLGPRRLNAPGVRFEDLPPIHAVVVSHSHYDHLDRST
                     VERLARTHRPRFFVPLGLKAWLADLGITDVEELDWWQSRTLRDVTFVCTPVQHWSGRT
                     PWDTDRRLWSGWALAGRERRLFFGGDTGYWDGFRDVGARLGPFDLVAVSIAAYEPPEI
                     MQHTHTTPEQSLELFAAVRGRRFVAMHWGTFDLGDEPIAEPPRRLQAEAQRLGLDDER
                     VWILRHGETRAW"
     gene            complement(3665..4915)
                     /locus_tag="VLF19_02100"
     CDS             complement(3665..4915)
                     /locus_tag="VLF19_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92071.1"
                     /translation="MRLAVDPHLKILHTVSEAVSHSLDVDEVVRTAVEALTSVTGHES
                     SSLHLLSDDGTRLLLKGERGLSPQLREINRSLPVGEGLIGRVAATGEMLCIDDNARDP
                     GVFAPARAAVRTDRIRGFVCVPIRSRGRILGTLSLGRQTAEGFDDREVALVQLTADQI
                     GIALDNARLYSESQRQLEELRRTQAQLLHAEKLSAIGELASGVAHEINNPLTTILGQA
                     HLVLADTDVKVNLRDRIRIIADETSRAARIVQNLLMFARHYIPERRPCALADQVRRVL
                     ELKIYQLQQDDIAVETDFAPCPPVYADENQIQQVLLNLVQNAHHAMSRQESPRVLTIR
                     VSAAGDHGRIEVLDTGHGIPPEVLPKVFDPFFTTKPAGKGSGLGLSVSYGIVVEHHGR
                     LTAANRPEGGAAFVVDLPFGAPAD"
     gene            complement(5013..5756)
                     /locus_tag="VLF19_02105"
     CDS             complement(5013..5756)
                     /locus_tag="VLF19_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92072.1"
                     /translation="MNVEAPGGAPVAETDALAARTFADKSAAFLRLAAGVAHELRNPL
                     AVILARAQLLALEIAQGRRPEPVKIESVLKIIEEQAIRASGIIESLSMFARPRRPVLD
                     ALDTATVVQDALQTLRQRAEAAGVTVEVAIEQAPVTLSADREQLVTALTHLACNALDA
                     MEAGGRLTVRVRRGAGSVEIVVADTGPGVAAGDAERIFEPFFSTKSEAAGLGLCVAQT
                     IAEAHGGALRLTASDTAGAEFVLALPARS"
     gene            complement(5929..>6100)
                     /locus_tag="VLF19_02110"
     CDS             complement(5929..>6100)
                     /locus_tag="VLF19_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSE92073.1"
                     /translation="DLTPRIHAGKLMQEVARAVGGTGGGRPDLAQGGAKDDSRLSELK
                     ARVLAYVGGRGQ"
BASE COUNT          758 a         2207 c         2239 g          896 t
ORIGIN      
        1 ccgctacatg gcctcgtcgt ggacgaaccc cgcgcggagc tggtcgtgga cccggcggcg
       61 gggtaacctc gtcgtgcggg cctcgttctc ggcgctcgag gacgtcgacc cggtgagcca
      121 ggtgttccgc gagcgcctgc ggatcgaggg catcgagaac ggcacacatc gggtctaccg
      181 tcagacccat ctgtcgcgca tggtgttccc tcaggagctt cgggccctcg tcgagctggc
      241 gggagacttc gagttcgtcc agtggttctt cggcttcaag ccccaccagg tgctggagcg
      301 gtcgcgccat ccgctgttga tggtcgtcgt actccggaag acgtagcccg gcggccatgc
      361 tcccggcggg cgcgatcgag gcggggcgcc ctgtcttcat tcgtgccccg acggccggcg
      421 atcgtgacga gctcgtggcc ctcgggcgct cgagccggcc ctccgcccgt gggtcgatcc
      481 gccccacgga gcgtggcttc ctcgttcgcg gccatcgcgc cgaggtgatc gtggtgccgt
      541 ggcgaaggcg attctgagac tcaagcggga gcgcgagcgc cgggccctga cccatccgtg
      601 gatcttcaag ggcgacgtgg cggacgtcac cgatgcgccg ccagacgccg tggtcaccgt
      661 ggtggacgct gccgggcgct tcgtcggtcg cggcttcttc aacccgcggc cggctctttg
      721 ctgccgcatc gtcacgtggg aggacgagcc gctcgacgac cacctgctcg cccgccgggt
      781 cgccgccgcc gtcgcccggc gtggagccac ggcgacgctc ggccgcctgg tgtggagcga
      841 ggccgacggc cttcccggcc tcgtcgccga ccgctacggc ccggtgctgg tcctgcagtg
      901 cctcacgcgg gcgatggcct cccaccgccc ggcgctcgtc gaggcgctcc gcgcggcgac
      961 ggcggacctg cccattttct cggcggacga ggcgacgccg gccgggctgg aaggcttcga
     1021 acccgtccgg ggctgggtgg accgggaggg gccggcgcgc gtgatcgtgg aggagacctg
     1081 ggtgcgtctc gccgtcaccc tcggaggcgg ccacaagacg ggtctctacc tggaccagcg
     1141 tgagaaccgg ccccgcgtgg gcgcgctggc gaccgggcgc gacgtgctcg atgccttcgc
     1201 gtacaccgcg gccttcgcct gccacgccct ggccgccggc gcccgccggg ccctctgcct
     1261 ggagtcgtcg ccggaggcca tcgcgggcgc gcgcgagaac ctggcgctca acggcctcgc
     1321 cgaccgcgcg gagatcgtcg cgggcaacgc cttcgacgag ctgcggcgtc tcgagcgcac
     1381 gcgggcgcgc ttcgggctga tcgtgctgga cccgccgccc ttcgcgcgcg gccggacgac
     1441 gctggacgcc gccgcccgcg gctacaagga gatcaacctg cgcgcgatgc ggctgctcga
     1501 ccgcggtggc gcgctggcga cgttctcgtg ctcgcaccac gtctcgggtg ggttcttcga
     1561 ggagatctgc cgcgcggcgg ccacggatgc cggcgtccgg ctgcggatcg tggcgacgct
     1621 cggccagccc gccgaccacc cgatcgtgtt gacggtgccg gagaccggct acctcaaggg
     1681 cctgctcctg caggcgtggt agtctctgcc atgaccgcgc ccggcctgcc cccgatcgac
     1741 ggcacctggt actgcgatct ctgggacccg gacctcaaga tcctccaccg cgtccgcacc
     1801 acgctgttcg acggcgcctc cgcgttccag acgatcgagg tggccgacac gtgcggcttc
     1861 gggcggatgc tgatcctcga cggcctgccg caggccgccg agaaggacga gatggtctac
     1921 gcgaaggcgc tcgcatggcc ggcgctgctc gccgtccccg ggcccaagcg cgtgctcgtc
     1981 accggcggtg gcgacggcca cgtcatgcgg gaagtgctgc gcttccccag cgtcacctcg
     2041 gtcgtcgtct gcgacatcga ctcgatggtg accaaggtga cccaggagca catgccgttc
     2101 atgtgggatg gcgccgagcg cgaccggcgc gcggagatcc gtcacgccga cgcctgggaa
     2161 ttcctgggct ctgcggcgcc cggctcgttc gaggtggtca tctccgacat caccgacccg
     2221 gccggcgagg gcagcgcctc ccatcacctc tactcgcccg cgtacttcca gcggatcagg
     2281 cgctgcctgg cgccgggcgg cgtctgcgcg gcgcaggccc aggagctgtc cgtgcgcgac
     2341 ttcgcgcacc acgcgcggct gcgccggtgc gtcgccagcg tcttcgccgg ggtacggtcc
     2401 ggccatgtct acatgccgtc cttcggctac ccggaggggc tgctcttcgc ttccgatgac
     2461 cccgcacgcc tcggcctcgg ccgcgtggag gtcgaggcgc gcctgcgcga ggcgggactc
     2521 gccgccgacg cccacttcga cgcggcggtc cacgcggcga tgttcaccct ccccccgctg
     2581 atccgcgccg cgctcgagaa gtcctgagag gcgccggctc accaggcccg cgtctccccg
     2641 tgacgaagga tccacacgcg ctcgtcgtcg agcccgaggc gctgcgcctc ggcctggagc
     2701 ctccgtggcg gctcggcgat gggctcgtcg ccgaggtcga acgtgcccca gtgcatcgcg
     2761 acgaagcgcc ggccgcggac ggccgcgaag agctcgagcg actgctccgg cgtcgtgtgg
     2821 gtgtgctgca tgatctcggg tggctcgtac gccgcgatcg acaccgcgac cagatcgaag
     2881 gggccgagcc tggcgcccac atccctgaag ccgtcccagt agcccgtgtc cccgccgaag
     2941 aagaggcgcc gctcccggcc cgcgagcgcc cacccgctcc agagtcggcg gtcggtgtcc
     3001 cagggcgtcc gccccgacca gtgctgcacg ggcgtgcaga cgaaggtgac gtccctcagg
     3061 gtcctgctct gccaccagtc gagctcctcg acgtcggtga tcccgaggtc cgcgagccac
     3121 gccttcaggc cgagcgggac gaagaagcgc gggcggtggg tccgcgccag gcgctccacc
     3181 gtggaccggt cgaggtggtc gtagtggctg tgcgagacga cgaccgcgtg gatcgggggg
     3241 aggtcctcga agcgcacgcc cggcgcgttg aggcggcgcg ggcccagcca cgacgtgggc
     3301 ccggcgcgct cggaccagat gggatccgtg aggacgttga cgccgtcgag ctggaccagg
     3361 agcgtggcgt gccccaccca cgtgacggtc ggtctcccgc tgttcgcccg caacgcggcg
     3421 ccgtcgttgg cagtgcgcgg caggtccggg accggccggt tggcgctgat cccgcgccag
     3481 agccggctca gccggaactt ctggatcgcc cagaacgtgg ggtggacgaa ggcgggattc
     3541 gggttcgcga atccccgctc gcgatgatgc ggcggcgccc ctggcaccgg cccgccgacg
     3601 cccgcgcacc ccgcacagac gagcaccgcc agcaccagcg cggacgtcga acgggccatt
     3661 cggctcagtc ggccggcgcg ccgaacggca ggtcgaccac gaaggcggcg ccgccctcgg
     3721 gccggttcgc ggcggtgagg cgcccgtggt gctccaccac gatgccgtag gaaacgctga
     3781 gcccaagccc gctgcccttt cccgccggct tcgtggtgaa gaacgggtcg aagaccttcg
     3841 gcagcacctc cggcgggatc ccgtggcccg tgtccagcac ctcgatgcgc ccgtggtcgc
     3901 cggccgcgga cacgcggatc gtgagcacgc ggggcgactc ctgccgtgac atcgcgtggt
     3961 gggcgttctg gacgaggttc agcagcacct gctggatctg gttctcgtcg gcgtacaccg
     4021 gcgggcacgg cgcgaagtcg gtctcgacgg cgatgtcgtc ctgctggagc tggtagatct
     4081 tcagctcgag cacccgccgc acctggtcgg cgagcgcgca cggccggcgc tccgggatgt
     4141 agtggcgggc gaacatcagg aggttctgca cgatccgcgc cgcgcgcgag gtctcgtcgg
     4201 cgatgatccg tatccggtca cggaggttca ccttcacgtc ggtgtccgcg agcaccaggt
     4261 gcgcctgccc gagaatggtc gtgagggggt tgttgatctc gtgcgccacg cccgaagcga
     4321 gctcgccgat ggccgagagc ttctccgcgt gcaggagctg ggcctgggtg cgccggagct
     4381 cctcgagctg ccgctgcgac tcggagtaca ggcgggcgtt gtcgagcgcg atgccgatct
     4441 ggtcggcggt gagctgcacc agggcgacct cgcgatcgtc gaagccttcc gccgtctgcc
     4501 ggcccagcga gagcgtgccg aggatgcgcc cgcggctgcg gatgggcacg cagacgaagc
     4561 cacggatccg gtcggtccgt acggcggcgc gggcgggcgc gaacacgccg gggtcccggg
     4621 cgttgtcgtc gatgcagagc atctcgccgg tcgcggcgac gcggccgatc agcccctcgc
     4681 ccacgggaag gctccggttg atctcgcgca gctgcggcga gagcccgcgc tcgcccttca
     4741 ggagcagccg cgtgccgtcg tccgagagca ggtgcaggct ggagctctcg tgaccggtca
     4801 cgctggtgag cgcctcgacc gccgtgcgga ccacctcgtc gacgtcgagg ctgtggctga
     4861 cggcctcgga gacggtgtgg aggatcttga ggtgcgggtc gacggcgagc ctcagccggc
     4921 cccgagtgtc gtgcgggcga cgccgcccgc ggcctcgctc gtggagcgca cggcggccgg
     4981 caggtcgcgc cccgcgcttt gggccatggt gatcagctcc tggccggcag cgcgagcacg
     5041 aactcggctc cggcggtgtc tgatgccgtc agccggagcg cgcccccgtg cgcctcggcg
     5101 atggtttgcg cgacgcagag tccgagcccg gcggcctcgg acttggtcga gaagaacggc
     5161 tcgaagatgc gctcggcgtc gccggcggcg acgccggggc cggtgtcggc caccacgatc
     5221 tccacgctgc ccgcgccgcg cctcacgcgc acggtgagcc ggccgccggc ctccattgca
     5281 tccagcgcgt tgcacgcgag gtgcgtgagc gcggtgacga gctgctcgcg gtcggcgctg
     5341 agcgtcaccg gcgcctgctc gatcgcgacc tcgaccgtga cccccgcggc ctcggcgcgc
     5401 tggcggagcg tctggagcgc gtcctgcacg accgtggccg tgtcgagcgc gtcgagcacg
     5461 ggccggcgcg ggcgcgcgaa catcgacagg ctctcgatga tccccgaggc gcggatcgcc
     5521 tgctcctcga tgatcttcag cacgctctcg atcttgaccg gctcggggcg acggccctgc
     5581 gcgatctcga gcgccaggag ctgggcccgg gcgaggatca ccgcgagcgg gttgcgcagc
     5641 tcgtgcgcca cgccggccgc cagtcggagg aacgccgcgc tcttgtcggc gaaggtccgg
     5701 gcagccagtg cgtcggtctc ggccacgggc gcgccgccgg gggcctcgac gttcatgccg
     5761 agagctattc tagccggact gcggggccgg cccctaagac cccgtcgggc ggcgtatcga
     5821 ggcgtgcccc ggccgggccg ggggcgccga gcgctggggt gtcggggcca cgtcggggcc
     5881 ccgtgccctg ggaaccggtc gggattcggg acatcgaggt ggccacggtc attgaccgcg
     5941 gccgccgacg taagccagca ccctggcctt cagctcggac agccggctgt cgtccttcgc
     6001 cccgccctgc gccaggtcgg gccgcccgcc gcccgtcccc cctaccgccc tggccacctc
     6061 ctgcatgagc ttgccggcgt ggatgcgggg tgtcaggtcc
//
LOCUS       DATIAZ010000063         8603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1725506, whole genome shotgun sequence.
ACCESSION   DATIAZ010000063 DATIAZ010000000
VERSION     DATIAZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725506"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VLF19_02115"
     CDS             <1..276
                     /locus_tag="VLF19_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HSE92074.1"
                     /translation="VYRVGGHTAGLQIVTVDTARGTVVLTSDASHFYRNVETRQPVQI
                     ITSLPEMLAAFETIHALAGPQKLIVAGHDPQVAERFKPVEDGIIKIA"
     gene            495..1772
                     /locus_tag="VLF19_02120"
     CDS             495..1772
                     /locus_tag="VLF19_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92075.1"
                     /translation="MQAGLRTALASLVLGTLLALVPALDGAGAQPKPIRIGEINSYSG
                     VATVYTFPYKEGLALATKEINDAGGVLGRPIEFIHRDDKLKPDEAVKAARELVLQDKV
                     DFLAGCISSGVGLAISAYARDAKVLYFATHCQTSRLTWDDGHKYVAHTTNNVNQYVRV
                     AARKASTLPIKKWANISPDYEYGHNMWEEFSTYLKKLKPDVEFVQVNWPKLGETDHSS
                     YITALLQSGAEGLFSSLWGSQSIAFVKQARPFGLFDKMRYVAPSVGNPDELDPLGKEA
                     PVGAIATPFAWYDPGLNKLHPKLAAWTKKYTEWAKAKGLKDPQPKLGASWGYASAYII
                     AEAIKRARSTETDKVIAALSEGFEMDFPWGKVVMRGCDQQALPPQWVGVVKLDAQGKP
                     ITTDVEEVQGKDVARSCDEVKDLRATATARQKK"
     gene            1824..2738
                     /locus_tag="VLF19_02125"
     CDS             1824..2738
                     /locus_tag="VLF19_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92076.1"
                     /translation="MDLITGVFPWITNGPLILVQVMSGLSVAMFLFLVSVGMSLIFGV
                     TRIVNLAHGSFYMVGAYLMVTLAEMLPEHPLSFLAALLLAPLGVAILGGAVEVALLRR
                     IYHRDPLMQLILTFGLILIIGTLVLLVWGPDNKSVSRPDALAGAVSIFGQPFPSYYLL
                     VIALAPVVGVGLWLVFYRTRWGMLIRAATVDREMLGALGVDVATLYTQVFVFGSWLAG
                     LGGALAAPTVAVALGMDSDVLTTAFVVVVVGGLGSFAGTFISALLVGELQSFGILVFP
                     AVSIVLLFAIMAAVLIVRPWGLLGRPED"
     gene            2708..3667
                     /locus_tag="VLF19_02130"
     CDS             2708..3667
                     /locus_tag="VLF19_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92077.1"
                     /translation="MGPARASGGLAIVAARWGIGLTALLVLPLVLPSFLVVLMNEIAI
                     VALFATAFNLLMGFGGMVSFGQAAYFALGAYAAAILVTHAAVPMLPALAAAPFVAAAG
                     ALLFGFFIVRLTHTYFAMLSLAFAQIVFTAIFKWTSLTGGDNGLLNIWPPEWLKSPRA
                     YCYFTLVVVGLAILALHAIVASPFGYALKAIRENPRRARFIGVNVRRHQLVAFVLSGF
                     FSGLAGGLFAFFNGSVFPDFAFLPKSFEPLVVALLGGVQSFFGPLAGAVGFKILETFI
                     ARQWPVYWPLFLGSAVVLVIILLPQGFVGLLQRRTWRIRATAR"
     gene            3640..4446
                     /locus_tag="VLF19_02135"
     CDS             3640..4446
                     /locus_tag="VLF19_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92078.1"
                     /translation="MADPGNGTLVLHVVGLAKHFGGVQAVNGVNLAVPEGDVRAIIGP
                     NGAGKTTLFNLITGDLPHDAGQIYFRGEEVSGLPPQVLCRRGVGRTFQITSIFRRLTT
                     VENVQTALLTHHRRHFDILRPARRLYRSEAMILLDRVGLADQAAKPGGILSHGDQRRL
                     ELAIALASEPHLLMLDEPTAGMAPKERHEIMALAAGIATDAGLTILFTEHDMDVVFAV
                     AKRITVLHQGAVIAEGSPLQVRANVEVQRVYLGAQPLGRQAPGREAVTPQ"
     gene            4553..5272
                     /locus_tag="VLF19_02140"
     CDS             4553..5272
                     /locus_tag="VLF19_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92079.1"
                     /translation="MTPLLEVRGLDAYYGDSHILYGLSLDVAEGEVVCLLGRNGAGKS
                     TTLKSIIGLVPPRSGRVALRGRALTGQPPFRIARAGLGYVPEERRIFGNLSVAENLEV
                     ARRTWTNDRGAPQAWTAERVLELFPHLRAMRDRPGGRLSGGEQQMLTIARTLMGSPAV
                     LLLDEPSEGLAPLVVEMLAEQLTNLKRTGLAMLVAEQNVHFVSGLGDRVYILENGAVR
                     YQGPMSAFLADDQVRRAHLAV"
     gene            5503..7602
                     /locus_tag="VLF19_02145"
     CDS             5503..7602
                     /locus_tag="VLF19_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSE92080.1"
                     /translation="MSWRVGVDIGGTFTDVVLAEDARGVLAVAKVPTTPRDLTRGVLD
                     ALGAATREHGVAPDQVTLLAHATTVVTNALLEARGARTALITTRGFRDVLELRRSARA
                     SLYDLFQDGPTVLVPRHLRLEVAERVDARGAVVQPLVAEDVDDAIDFIKRHDVQAVAV
                     CLLFAFLNDAHERAIGARLRAALPDLPVFLSSEVLPEIREFERTSTTAVCAYVSPILA
                     AYLERLEAAVTGRGFPAPLIMSSAGGVLGVAEALRMPAMVVESGPAAGVMAAAVAGRT
                     LGEPRLISFDMGGTTAKASLIENGEITTTSEYEVGGGGHVRRWLHGTGHPIRVPVIDL
                     AEVSAGGGSIAWLDPGGALRVGPESAGAEPGPVCYGRGGQRPTVTDADLVLGYLNPVA
                     LLGGALPVDRGRAAAAIDDAIARPLGLGLDAAAAGIVDVVNAGMAAALRIVSVERGFD
                     PREFSLVAFGGAGPVHAARLAEELAIPRVIVPPIPGGFSALGLVASDLRRDYAKTFYA
                     PLASAPLRDIAVVYEDLESAARAVLRTAGVPESRWELSRAADVRYSRQAYELTVPLAP
                     GPITRATVERLAADFHDKHRSMYGHASPDEPVQLVTLRLSAVGRKAGLDLGRSGCAVA
                     AGAAVSSPPVPTARDVHFRETGRVRCDVMDRAGLAPGRKTRGPTIIESMDTTVVVPPG
                     WDVRADARGFILLERNA"
     gene            7602..>8603
                     /locus_tag="VLF19_02150"
     CDS             7602..>8603
                     /locus_tag="VLF19_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSE92081.1"
                     /translation="MRETDPATFEVVKNALYAAAEEMKIVLAKTAYSPLLKVAGDYSC
                     GLFDVRGEMVAQGPDLPIHLGSMPLAVKAVIQAWPVVQPGDVFIHNDPYFGGSHLPDV
                     NVVTPAFHAGTLLGFACVRAHWPDIGSATPGSYGATTEIFGEGLRLPPVRLYAAGVLN
                     RDVEDIIFTNVRTPEERRGDLRAQLAANRRGTARLAALAEKHGAERLLHLMQQVIDYS
                     ERMMRAMLADLPDGTATFEDFCDGDGILEEGEKEDRTFRIRMRVTKHGDRLTVDFAGT
                     DPQVPGPMNAPLNVTASGIYAAVKMVADPGDLVPPNSGCWRPVRLVADPGTVVNAMPP
                     "
BASE COUNT         1186 a         3107 c         2976 g         1334 t
ORIGIN      
        1 gtgtaccgcg tgggcggcca caccgcggga ttgcagatcg tcaccgtcga caccgcccgc
       61 ggcacggtcg tcctcacctc cgacgcctcg cacttctacc gcaacgtgga gacgcgccag
      121 cccgtgcaga tcatcacgag cctgccggag atgctcgcgg ccttcgagac catccacgcc
      181 ctggccggcc cgcagaagct catcgtggcc ggtcacgacc cccaggttgc cgagcgcttc
      241 aagcccgtgg aggacggcat catcaagatc gcgtagcgcg aacccccttg acccacggct
      301 cgggggggtg cagagtgccc ccactttttc aaccgtccct cccgtcggga ggcagcatgc
      361 ccctggctcg ggtctcagga cagttcttcg acgcccatcc cgatcgccgc catcggatcc
      421 tggcggccgt gtctcgcctc actgcccgac cccggccccc aacccgtccg cgtgtctcca
      481 gaaggaggcg caccatgcaa gcagggctcc ggaccgcgct cgcgagtctc gtcctcggca
      541 ccctcctggc gctcgtaccg gccctcgatg gcgccggcgc ccagcccaag ccgatccgga
      601 tcggcgagat caactcctac agcggcgtgg cgaccgtcta cacctttccc tacaaggaag
      661 ggctggcgct ggccaccaag gagatcaacg acgccggcgg ggtgctgggc cgcccgatcg
      721 agttcatcca ccgcgacgac aagctcaagc cggacgaggc cgtgaaggcc gcgcgcgagc
      781 tggtgctgca ggacaaggtc gacttcctgg ccggctgcat cagcagcggc gtcggcctgg
      841 ccatctcggc gtacgccagg gacgccaagg tcctctactt cgccacccac tgccagacct
      901 cgcggctcac ctgggacgac gggcacaagt acgtcgccca caccacgaac aacgtgaacc
      961 agtacgtgcg ggtcgcggcc aggaaggcgt cgaccctgcc gatcaagaag tgggcgaaca
     1021 tctcgcccga ctatgaatac ggccacaaca tgtgggagga attctccacc tacctcaaga
     1081 agctcaagcc cgacgtggag ttcgtccagg tcaactggcc caagctgggc gagaccgacc
     1141 acagctccta catcacggcg ctgctgcagt ccggtgcgga ggggctcttc agctcgctct
     1201 gggggtcgca gtcgatcgcc ttcgtgaagc aggcgcgacc tttcgggctg ttcgacaaga
     1261 tgcgctacgt ggccccctcc gtgggcaatc cggacgagct cgacccgctg ggcaaggagg
     1321 cgcccgtcgg cgccatcgcc acccccttcg cctggtacga ccccgggctg aacaagctgc
     1381 acccgaagct cgcggcgtgg acgaagaagt acacggagtg ggcgaaagcg aaaggcctca
     1441 aggacccgca gcccaagctg ggcgcttcct ggggctacgc gagcgcgtac atcatcgccg
     1501 aggccatcaa gcgtgccagg agcaccgaga ccgacaaggt catcgccgcg ctgagcgagg
     1561 gcttcgagat ggacttcccc tggggcaagg tggtcatgcg gggctgcgac cagcaggcgc
     1621 tgccgccgca atgggtcggc gtcgtcaagc tcgacgccca gggcaagccc atcacgaccg
     1681 acgtcgagga ggtgcagggc aaggacgtcg cgcgcagctg cgacgaggtc aaggacctgc
     1741 gtgccacggc gacggccagg cagaagaagt agcggatccc gccggcgggg gccgcagccg
     1801 gcccccgcct ccgtgagccc ggcgtggacc tgatcaccgg cgtcttcccc tggatcacca
     1861 acgggccgct catcctcgtg caggtgatga gcgggctgtc cgtggcgatg ttcctgttcc
     1921 tcgtctcggt cgggatgtcg ctcatcttcg gcgtcacgcg gatcgtcaac ctcgcccacg
     1981 gtagcttcta catggtcggc gcctacctca tggtcacgct ggccgagatg ctgcccgagc
     2041 acccgctgtc cttcttggcc gcgctgctcc tggcgccgct cggcgtggcg atcctcgggg
     2101 gcgccgtgga agtcgcgctg ctccggcgca tctatcaccg ggacccgctc atgcagctca
     2161 tcctgacgtt cggcctgatc ctcatcatcg gcacgctcgt gttgctcgtg tggggtcccg
     2221 acaacaagag cgtgtcccgg ccggacgcgc tggcgggcgc ggtgtcgatc ttcggccagc
     2281 cctttccgtc ctactacctg ctggtgatcg cgctggcgcc cgtcgtcggc gtcggcctct
     2341 ggctcgtgtt ctaccggacg cgctggggca tgctcatccg cgcggccacg gtcgaccggg
     2401 agatgctggg cgccctgggc gtggacgtcg ccacgctcta cacgcaggtg ttcgtcttcg
     2461 gcagctggct ggccggcctg ggcggcgcgc tggccgcgcc gaccgtcgcc gttgcgctcg
     2521 gcatggacag cgacgtgctc acgaccgcgt tcgtggtcgt cgtcgtcggc gggctcggca
     2581 gcttcgccgg caccttcatc agcgcgctgc tcgtcggcga gctgcagtcc ttcggcatcc
     2641 tcgtcttccc cgcggtgtcc atcgtgctcc tgttcgccat catggccgcg gtcctgatcg
     2701 tccggccgtg gggcctgctc gggcgtccgg aggactagcc atcgtcgccg cgcgctgggg
     2761 gatcgggctg accgcgctgc tcgtgctgcc gctcgtgctc ccgagtttcc tcgtcgtgct
     2821 gatgaacgag atcgccatcg tggcgctgtt cgccaccgcc ttcaacctcc tgatggggtt
     2881 cggcgggatg gtctcgttcg gccaggccgc ctacttcgcg ctcggcgcct acgcggccgc
     2941 catcctcgtc acgcacgcgg cggtgcccat gctgccggcc ctggccgccg cgccgttcgt
     3001 ggccgcggcc ggggccctcc tcttcggctt cttcatcgtg cggctcaccc acacgtactt
     3061 tgccatgctg tcgctggcct tcgcccagat cgtcttcacc gcgatcttca agtggacgag
     3121 cctcaccggc ggcgacaacg gcctgctcaa catctggccg ccggagtggc tcaagtcgcc
     3181 gcgcgcgtac tgctacttca cgctggtcgt cgtcggtctg gccatcctgg cgctgcacgc
     3241 catcgtcgcc tcgcccttcg gctatgcgct caaggccatc cgggagaacc cgcggcgcgc
     3301 ccggttcatc ggcgtgaacg tgcgccgcca ccagctcgtc gcgttcgtgc tgtccggctt
     3361 cttctccggt ctggccggcg gcctgttcgc cttcttcaac ggcagcgtct tccccgactt
     3421 cgcattcctg cccaagtcgt tcgagccgct ggtggttgcg ctgctgggcg gggtgcagtc
     3481 cttcttcggc cccctggccg gggcggtcgg cttcaagatc ctcgagacct tcatcgcacg
     3541 gcagtggccc gtgtactggc cgctgttcct gggcagcgcg gtcgtcctcg tcatcatcct
     3601 gctgccccag ggcttcgtgg ggctgctgca gaggcggacg tggcggatcc gggcaacggc
     3661 acgctagtcc tgcacgtcgt cggcctggcc aagcacttcg gcggagtgca ggccgtgaac
     3721 ggggtgaacc tggccgtgcc cgagggcgac gtgcgcgcga tcatcggtcc caacggcgcc
     3781 ggcaagacga cgctcttcaa tctcatcacc ggcgacctgc cccacgatgc cggacagatc
     3841 tacttccgtg gcgaggaggt gagcgggctg ccgccccagg tcctctgccg gcgcggcgtc
     3901 ggccggacct tccagatcac gagcatcttc cgccgcctga ccacggtgga gaacgtgcag
     3961 acggcgctgt tgacacacca ccggcgacac ttcgacatcc tccgcccggc tcgccggctc
     4021 taccggagcg aggcgatgat cctgctcgac cgcgtcggcc tggccgacca ggccgccaag
     4081 cccggtggca tcctctccca cggcgaccag cggcgactgg agctggccat cgcgctggcc
     4141 agcgagccgc acctgctgat gctcgacgag cccaccgcgg ggatggcgcc caaggagcgg
     4201 cacgagatca tggcgctcgc cgccggcatc gcgaccgacg ccggcctgac catcctcttc
     4261 accgagcacg acatggacgt ggtattcgcc gtcgccaagc ggatcacggt gctgcaccag
     4321 ggcgcagtga tcgccgaggg cagcccgctg caggtgcggg ccaacgtcga ggtgcagcgc
     4381 gtgtacctgg gggcgcagcc gctcggccgg caggcgccgg ggcgggaggc ggtgacgccg
     4441 caatgatcgc cacggtggtg ccgagtcgat gcattccaac ggcaccgtcg ccggcgcccc
     4501 agccggctgt cctcgcggca gcggagcggc ccgacggccg ccggacgacg cggtgacgcc
     4561 gctgctcgag gtgcgcgggc tcgacgccta ctacggcgac agccacattc tctacggcct
     4621 gtcgctcgac gtggccgagg gcgaggtcgt ctgcctgctc ggacgcaacg gcgccggcaa
     4681 gtccacgacg ctcaagagca tcatcgggct cgtgccgccc cgctcgggcc gtgtcgcgct
     4741 gcgcggccgg gcgctgaccg ggcagccgcc gtttcgcatc gcgcgggcgg ggctcggcta
     4801 cgtgccagag gagcggcgca tcttcggcaa cctcagcgtg gccgagaacc tggaggtcgc
     4861 gcgccgcacg tggaccaacg accgcggagc gccgcaggcg tggacggccg agcgcgtgct
     4921 cgagctgttt ccccatctgc gcgccatgcg agaccgcccg ggcggccggc tgagcggcgg
     4981 cgagcagcag atgctgacga tcgcccgcac cctgatgggc agcccggccg tgctgctgct
     5041 cgacgagccg tcggaagggc tggcgccgct ggtcgtggag atgctggccg agcagctcac
     5101 gaacctcaag cgcacgggcc tcgcgatgct cgtcgccgag cagaacgtcc acttcgtcag
     5161 cgggctgggc gatcgcgtct acattctcga gaacggggcg gtgcgctacc agggcccgat
     5221 gtccgcgttc ctggccgacg accaggtgcg gcgggcccac ctcgcggtgt aggtcggaga
     5281 gcctcgacgg atcagcgctt gcctcgacgg acggccgtcg ctatcctcgc ccggcgacgg
     5341 tccggcgcct gccgttccgg cccgatccca gcgaggtgtg ccatgagtca gacgtctccc
     5401 tgccggtctc ccggggaggt cttggggccg gccgggactt ccacgggcgc gacaaggtca
     5461 tcatgaagtt cgcggtcggg gcgtgatgcg ggcgctggcc acgtgagctg gcgggtcggg
     5521 gtcgacatcg gcggcacctt caccgatgtg gtgctggccg aggatgcgcg cggcgtcctc
     5581 gccgtggcga aggtgccgac gaccccgcgt gacctcacac gcggggtgct ggacgccctg
     5641 ggcgcggcga cccgcgagca cggcgtggcg ccggaccagg tgacgcttct cgctcacgcc
     5701 accaccgtgg tcacgaacgc cctcctcgag gccaggggcg cgcgcacggc gctcatcacg
     5761 acccgcggct ttcgcgacgt gctcgagctg cgccgctcgg cccgcgccag cctctacgac
     5821 ctcttccagg acggacccac cgtcctggtt ccccgccacc tgcgactcga ggtcgccgag
     5881 cgcgtggacg cgcgtggcgc ggtggtgcag ccactcgtgg cggaggacgt ggacgacgcc
     5941 atcgacttca tcaagcgcca cgacgtccag gcggtcgccg tgtgcctgct cttcgcattc
     6001 ctcaacgacg cccacgagcg ggccatcggc gcgcggctcc gcgccgccct gcccgatctg
     6061 cccgtcttcc tgtcgagcga ggtgctaccg gagatccgcg agttcgagcg caccagcacc
     6121 acggctgtct gcgcgtacgt gagcccgatc ctggctgcct atctggagcg gctcgaggcc
     6181 gccgtcaccg gccgcgggtt cccggcgccc ctcatcatga gctcggcagg cggcgtgctc
     6241 ggcgtggcgg aagcgctgcg catgccggcc atggtggtgg agtcggggcc ggcggcgggc
     6301 gtcatggccg cggcggtggc cggccgcacc ctgggcgagc cgcgcctgat ctcgttcgac
     6361 atgggcggca ccacggccaa ggcgagcctg atcgagaacg gcgagatcac gaccacgtcc
     6421 gagtacgaag tggggggcgg tggccacgtc cgtcgctggc tgcacggcac cggccacccg
     6481 attcgcgtgc ccgtgatcga tctggccgag gtcagcgcgg gcggcgggag catcgcctgg
     6541 ctcgaccccg gcggtgccct gcgggtgggg ccggagagcg cgggggccga gccggggccc
     6601 gtctgctacg ggcgaggcgg ccagcggccc accgtcaccg acgccgacct cgtgctgggc
     6661 tacctcaacc cggtggcgct cctcgggggg gcgctgcccg tcgatcgcgg gcgcgcagca
     6721 gccgccatcg acgacgccat cgcgcgtccc ctggggctcg ggctcgacgc ggccgctgcg
     6781 ggaatcgtgg atgtggtcaa cgccggcatg gccgccgcgc tgcgcatcgt gtccgtcgag
     6841 cgcgggttcg acccgcggga gttcagcctg gtcgccttcg gaggggccgg gcccgtgcac
     6901 gcggcccggc tggccgagga gctcgcaatt ccccgcgtca tcgtgccgcc gatcccgggc
     6961 ggcttctccg cgctcggcct cgtggccagc gacctgcgga gggactacgc gaagacattc
     7021 tacgcgccgc tggcgtcggc gccgctgcgc gacatcgcgg tcgtctacga agatctggaa
     7081 tcggctgcgc gcgcggtgct gcgtacggcc ggcgtcccgg aatcccgctg ggagctgagc
     7141 cgcgccgccg acgtccgcta cagtcgccag gcctacgagc tgacggtgcc gctggcgccg
     7201 gggccgatca cgcgcgccac cgtcgagcgt ctggccgccg acttccacga caagcaccgg
     7261 tcgatgtacg ggcatgcgag tcccgacgag cccgtgcagc tcgtcacgct gcgcctgtcg
     7321 gcggtgggcc ggaaagcggg tctggatctc ggccggagcg gctgcgcggt ggctgccggg
     7381 gcggcggtct cgtctccgcc cgtgccgacg gcgcgcgacg tccacttccg ggagacgggg
     7441 cgcgtgcgtt gtgacgtgat ggaccgcgcc gggctggcgc ccggccggaa gacgcgtggc
     7501 cccacgatca tcgagtccat ggacaccacg gtggtggtgc caccgggctg ggacgtgcgc
     7561 gccgacgccc gcgggttcat cctcctggag aggaatgcct gatgcgtgag accgatcccg
     7621 cgacgttcga ggtcgtcaag aacgcgctct acgcggcggc ggaggagatg aagatcgttc
     7681 tcgccaagac cgcgtactcg ccgctcctca aggtggcggg ggactattcg tgcggcctct
     7741 tcgacgtccg cggcgagatg gtggcccagg gtccggactt gccgatccac ctcggctcga
     7801 tgccgctcgc cgtcaaggcg gtcatccagg cgtggcccgt cgtccagccg ggcgacgtgt
     7861 tcatccacaa cgatccctac ttcggcggca gtcatcttcc cgacgtgaac gtggtcacgc
     7921 ccgcgttcca cgcgggcacc ctcctcggct tcgcgtgcgt ccgcgcccac tggcccgaca
     7981 tcgggagcgc cacgccgggc agctacggcg ccaccaccga gatcttcggc gagggcctgc
     8041 gcctgccccc cgtgcgcctc tacgcggcgg gcgtcctcaa ccgggacgtc gaggacatca
     8101 tcttcaccaa cgtgcgcaca ccggaggaac ggcgcggcga tctccgggcc cagctcgccg
     8161 ccaaccgacg cggcacggcg cggctcgcgg ccctggcgga aaagcacggc gccgagaggc
     8221 tcctgcatct gatgcagcag gtcatcgact actcggagcg catgatgcgc gccatgctgg
     8281 ccgacctccc cgacgggacg gccaccttcg aggacttctg tgacggcgac gggatcctgg
     8341 aggagggcga gaaggaggac cggaccttcc gcatccggat gcgggtgacg aagcacggtg
     8401 accggctcac cgtggacttc gcggggaccg atccccaggt gccggggccg atgaacgcgc
     8461 ccctcaacgt cacggcgtcg gggatctacg ccgcggtgaa gatggtggcc gatcccggcg
     8521 atctcgtgcc gccgaactcc ggctgctggc ggccggtcag gctcgtggcg gacccgggta
     8581 cggtcgtgaa cgcgatgccg ccc
//
LOCUS       DATIAZ010000064         5492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_278193, whole genome shotgun sequence.
ACCESSION   DATIAZ010000064 DATIAZ010000000
VERSION     DATIAZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278193"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..381
                     /locus_tag="VLF19_02155"
     CDS             163..381
                     /locus_tag="VLF19_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HSE92082.1"
                     /translation="MRGLALVGVVLIVLGAVALAYQGITYTTQEKVVDLGPLKVEAQR
                     EKTIPLPPVLGGIAVAVGVVLLVMGLRR"
     gene            complement(454..1650)
                     /locus_tag="VLF19_02160"
     CDS             complement(454..1650)
                     /locus_tag="VLF19_02160"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92083.1"
                     /translation="MPRASRPLTGIRVLDLTRVLAGPFCSMILGDMGAEVIKVEEPGK
                     GDDTRRWPPFVGGEATYFMSVNRNKRSITLNLKAPEGLGILRRLAAKSDVVIENFRTG
                     TMERLGLGYAALAKRNRRLIYCSISGFGLTGPEAARGGYDLIVQGESGVMDLTGFPDG
                     PPVKVGNSIADLVAGMAAAHAITLALLARTKTKRGQRVEIAMLDVMASLLTYQAGIYF
                     GTGQRPARRGNQHPSIVPYEVFKAADAYLTLGVANNSLWRACCGALERKDLAEDPRFD
                     TEARRVENRATLVPLLNEILGAKSAHEWLERLEAAGVPAGRINTVAEVCESEHLKARG
                     MIVRLPHPTAGEVTVMGVPLRLHGTPGAVVSPPPRLGEHTDAVLKRVLGMKPSRLRRL
                     RAAAVL"
     gene            complement(1634..2065)
                     /locus_tag="VLF19_02165"
     CDS             complement(1634..2065)
                     /locus_tag="VLF19_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSE92084.1"
                     /translation="MIETIPASRCPRCGFVAAPPAPFCPRHAVSMTPTAVAGVGEIVS
                     FTTLHSPPEGFRSPLHIALIALEGGARFVCHGSQTRGVRIGSRVAIEAIDDVYYFSSL
                     SALDRARMFWRRTGRAGDRVNAMTRSVVKRIFRGGSSAASQ"
     gene            complement(2062..3183)
                     /locus_tag="VLF19_02170"
     CDS             complement(2062..3183)
                     /locus_tag="VLF19_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92085.1"
                     /translation="MDVWIAGAGMTHIGRRAESLQRLMAAAAHAALADAGVERPDAIV
                     VAAMNPEEFVGDGNFASNVTTHMGFAEVPAIRIETATSSGAAAFYAGVAQVAAGLARR
                     VLVVGGEKMTHLPTPRVSELIGRSIDPYERSYGATMPALAGLITRALMTRHGVGEREI
                     GQVAVKNHANAARNPYAHFREPVTLEQVMASRMVADPLRLLHCCPISDGAAAVLVTAE
                     RAPVRVAGIGQGTDTLAVRHRHELTSFRATQMAARAAFAMAGFGPARVDFAELHDAFT
                     PFELIALEDIGLVPPGKAARATLDGETALDGRLPINPSGGLKARGHPLAATGVAQIVE
                     CVWQLTGRAEGRQVSGRVALAHSIGGLATNNWVTLLQGA"
     gene            complement(3296..3547)
                     /locus_tag="VLF19_02175"
     CDS             complement(3296..3547)
                     /locus_tag="VLF19_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSE92086.1"
                     /translation="MMIAPKPTPVPPIRSAPATYHGAVSEFKRRLIETTLRQAQGNRT
                     HAARALGLQRTYLLRLIRELGVAAPPPPERRGRAAVAAR"
     gene            complement(3722..3988)
                     /gene="fdxA"
                     /locus_tag="VLF19_02180"
     CDS             complement(3722..3988)
                     /gene="fdxA"
                     /locus_tag="VLF19_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HSE92087.1"
                     /translation="MPYIIAEPCINVKDKACVEVCPVDCIYEGPDMLYIHPDECIDCG
                     ACEPVCPVKAIFAEDETPEQWKRFIDLNKQFFKDNPGVQPAKKD"
     gene            complement(4109..5011)
                     /locus_tag="VLF19_02185"
     CDS             complement(4109..5011)
                     /locus_tag="VLF19_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92088.1"
                     /translation="MAVTDRALDTVTESQDRTQGRWVAATRDFVRRRPLGALGAVVIV
                     MMTVVAALAGVIAPYDPLATDYGGMLAAPSGAHWLGTDAFGRDVLARIIYGSRTALLV
                     GLGASFFGATLGALLGVASAYFGGRVDLLMERVMDILLSFPLIILALAVVAVLGTGAI
                     NVMFAIAIPMVPRCARVVRSTALTIREMPYVDAARAAGFSHGRIILRHMLPNVMAPYL
                     IMLTAFLGQAILLEASLSFLGLGVQEPTAAWGLMLRGAAVEFAETAPWMAIFPGLAIS
                     LAVFAFNLFGDSLRDALDPRLRTQ"
     gene            complement(5011..>5492)
                     /locus_tag="VLF19_02190"
     CDS             complement(5011..>5492)
                     /locus_tag="VLF19_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92089.1"
                     /translation="LVFFKWLPPMVYTPFWVNPWQNLAQLVWPAVAVGYRYSAVATRM
                     TRSAMLEVLREDYIRTARAKGLWLKLILSRHALKNAMLPVLTVIGLEFAFLLGGLVVT
                     EQVFNLNGLGLLFVEAIAHRDYTLTQALVMIVAFVFILVNFLTDIAYAWLDPRIRYR"
BASE COUNT          859 a         1883 c         1983 g          767 t
ORIGIN      
        1 tgggtgccgt cgcgtagcgt cgtgtcgtag atcgtgaccg tggaccgcat cggctgcctc
       61 cgctcgatcg aatgcgtccc gacatcatga gccagcgccg gaaggggtgt caagattgcg
      121 cggggccgcg tacgatgagg ccgagagccc cggaggtggg acatgcgagg actggcgctg
      181 gtgggagtgg tcctgatcgt gctcggcgcg gtggccctgg cctaccaggg catcacctac
      241 accacccagg agaaggtcgt ggacctcggt ccgctcaagg tcgaggcgca gcgggagaag
      301 acgatcccct tgccgcccgt cctgggcggc attgccgttg ccgtcggcgt cgtcctcctc
      361 gtcatgggcc tcagacgcta gaccgctcgg aggggcttcg ccgaaggggc ctgattcatc
      421 ggaggtggcc gggcccgtcg agaccccctc cgatcacagg acggcggcgg cgcggagccg
      481 gcgcagccgg gagggtttca tgccgaggac gcgcttgagc accgcgtcgg tgtgctcgcc
      541 caggcgcggc ggcggggaca cgacggcgcc cggcgtgccg tgcagcctca gcggcacgcc
      601 catgaccgtc acctcgcccg ccgtcgggtg cggcaggcgg acgatcatgc cgcgcgcctt
      661 caggtgctcg ctctcgcaca cctcggcaac ggtgttgatg cgcccggccg gcacgccggc
      721 cgcctcgagc ctctcgagcc actcgtgggc gctcttcgcg ccgaggatct cgttcagcag
      781 ggggaccagc gtggcccggt tctccacacg cctggcctcc gtgtcgaagc gggggtcctc
      841 ggcgaggtcc ttgcgctcga gcgccccgca gcatgcgcgc cacagcgagt tgttggcgac
      901 accgagcgtg agatacgcgt cggcggcctt gaagacctcg tagggaacga tcgacggatg
      961 ctggttgccc ctgcgcgccg ggcgctggcc cgtgccgaag tagatgcccg cctgataggt
     1021 gaggagcgag gccatcacgt cgagcatggc gatctccacg cgctggccac gcttcgtctt
     1081 cgtgcgcgcc aggagcgcga gcgtgatggc gtgggccgcg gccatgccgg cgacgaggtc
     1141 ggcgatggag ttgccgacct tcacgggcgg gccgtcggga aagcccgtga ggtccatgac
     1201 gccggattcg ccctgcacga tcaggtcgta gccgccccgg gcggcctccg gcccggtcag
     1261 cccgaatccg gagatcgagc agtagatcag ccgccggttg cgcttcgcga gcgccgcgta
     1321 gccgagcccc agccgctcca tggtcccggt gcggaagttc tcgatcacca cgtcgctctt
     1381 cgctgcaagc cgccggagga tcccgaggcc ctccggggcc ttgaggttca gggtgatcga
     1441 ccgcttgttg cggttcaccg acatgaagta cgtcgcctca ccacccacga agggcggcca
     1501 gcggcgcgtg tcgtccccct tgcccggctc ctcgaccttg atgacctcgg cgcccatgtc
     1561 gccgaggatc atcgagcaga atgggccggc gaggacccgc gtgaggtcga gcacgcgaat
     1621 cccggtcagc ggtctactgg ctcgcggcac tggaccctcc ccggaagatg cgcttgacga
     1681 cgctgcgggt catcgcgttc acccggtcgc cggcgcggcc ggtgcgccgc cagaacatgc
     1741 gtgcgcggtc gagcgccgac aggctcgaga agtagtagac gtcgtcgatc gcctcgatgg
     1801 ccactcgcga gccgatgcgg acgccccgcg tctgcgaccc gtggcacacg aaccgcgcgc
     1861 caccctccag cgcgatcagc gcgatgtgta gcggcgagcg gaagccctcg ggcggtgagt
     1921 gcagcgtcgt gaacgagacg atctcgccca cgccggccac ggccgtcggc gtcatcgata
     1981 cggcgtgccg ggggcagaac ggcgccggcg gcgccgcgac gaagccgcag cgaggacagc
     2041 gcgaagccgg gatcgtctcg atcatgcccc ctgcaggagc gtcacccagt tgttcgttgc
     2101 cagcccgccg atcgagtgcg cgagcgcgac ccgacccgac acctgccgtc cctccgcgcg
     2161 gcccgtgagc tgccagacgc actcgacgat ctgggccacc ccggtggcgg ccagcgggtg
     2221 gccacgggcc ttgaggccgc cagaggggtt gatcggcagc cggccgtcga gcgcggtctc
     2281 gccgtcgagg gtcgcgcgcg ccgccttgcc gggcggaacg agcccgatgt cctccagcgc
     2341 gatcagctcg aatggcgtga aggcgtcgtg cagctcggcg aagtccacgc gcgccgggcc
     2401 gaagccggcc atggcgaacg ccgcgcgggc ggccatctgc gtggcccgga agctggtcag
     2461 ctcgtgacgg tggcggacgg cgagcgtgtc cgtgccctgt ccgatgccgg ccacccggac
     2521 cggcgctcgc tccgcggtga ccaggacggc ggccgcgccg tcggagatgg gacagcagtg
     2581 caagagccgg agcgggtcgg cgaccatccg gctcgccatc acctgctcca gcgtcacggg
     2641 ctcgcggaag tgcgcgtagg gattgcgggc ggcgttcgcg tgattcttga cggcgacctg
     2701 accgatctcg cgctcgccca cgccgtggcg cgtcatcagg gcgcgcgtga tcaggcccgc
     2761 cagggccggc atggtcgcgc cgtaggagcg ctcgtaggga tcgatcgacc gcccgatgag
     2821 ctcggagacg cgcggcgtcg gcaggtgcgt catcttctca ccgccgacga cgaggacccg
     2881 acgcgccaga cccgcggcga cctgggcgac gcccgcatag aacgccgccg cgcccgacga
     2941 ggtggccgtc tcgatgcgga tcgccggcac ctcggcaaag cccatgtggg tcgtgacgtt
     3001 ggaagcgaag ttgccgtcgc cgacgaactc ctcggggttc atggccgcca cgacgatcgc
     3061 gtcggggcgc tcgacgccgg catcggcgag cgcggcgtgg gccgccgccg ccatcagcct
     3121 ctgaagactt tcggcccggc ggccgatgtg cgtcatgcct gcgccggcga tccagacgtc
     3181 catcatcgcc cttgtgtcca ttgtagcggc tggtgggcgc agggcgtgaa cggcgggtgc
     3241 gagcggcctc gcgtcgcagg gggcctgcgc ggcctcctgc gaaccgggtc actccctacc
     3301 gcgcggccac ggcggcgcgc ccgcgccgct cgggcggcgg gggcgccgcc accccgagct
     3361 cgcggatgag ccggagcagg tacgtgcgct gcaaccccag cgcacgggcc gcatgcgtgc
     3421 ggttgccctg cgcctggcgc agcgtggtct cgatgaggcg ccgcttgaac tcggagacgg
     3481 cgccatgata cgtggcgggc gcggagcgga tcggcggtac cggagtgggc ttgggggcga
     3541 tcatcatgcc ggcagcacat cgcaacgcgc gtgccacaac gcaacatcgc gttttcccga
     3601 cgccggcgcg acggaagccg ccgggacatg cacagcccac cgggcgccgt gccgcgctct
     3661 ctgtgcaaaa ggagagggcc cggcggccgg gccctcggcg ctgcgcgaac tccggagcgg
     3721 ctcagtcctt cttcgccggc tggaccccgg ggttgtcctt gaagaactgc ttgttgagat
     3781 cgatgaagcg cttccactgc tccggcgtct cgtcctccgc gaagatcgcc ttcaccgggc
     3841 acacaggctc gcaggcgccg cagtcgatgc actcatcggg gtggatgtag agcatgtccg
     3901 gcccctcgta gatgcaatcg accgggcaca cctcgacgca ggccttgtcc ttcacgttga
     3961 tgcacggctc ggcgatgata tacggcatcg gatatccccc ctctggagtc actcaagcct
     4021 ggtcggacca cgtgaggttg tacatgaccc ggcgcagcgt gtcaagcaag cttatcgcag
     4081 acttagccgc atcccgcggc ccggcggatc actgcgtccg caagcgggga tcgagcgcgt
     4141 cgcgcagcga gtcgccgaac aggttgaagg cgaagacggc gaggctgatg gccaggcccg
     4201 ggaagatggc catccacggc gcggtctcgg cgaactcgac ggcggcaccg cggagcatga
     4261 gaccccaggc ggcggtcggc tcctggacgc cgagcccgag gaacgacagt gaggcctcga
     4321 ggaggatcgc ctggcccagg aacgcggtga gcatgatcag gtagggcgcc atcacgttcg
     4381 gcaacatgtg gcgcaggatg atccgtccat ggctgaagcc ggcggcgcgc gccgcgtcca
     4441 cgtagggcat ctcgcggatc gtcagcgccg tggaccgcac gacccgggca cagcggggca
     4501 ccatcgggat cgcgatggcg aacatgacgt tgatggcgcc ggtgcccagg acggcaacga
     4561 cggcgagcgc caagatgatg agcgggaacg acagcaggat atccatcacc cgctccatca
     4621 agagatccac gcgcccgccg aaataggcgc tggccacgcc gagcagcgcg cccagcgtgg
     4681 ccccgaagaa cgaggcgccg aggcccacca gaagcgccgt gcgcgagccg tagatgatgc
     4741 gcgccagcac gtcgcgcccg aaggcgtcgg tgccgagcca gtgcgcgccg ctgggcgccg
     4801 ccagcattcc cccgtaatcc gtggccagcg ggtcgtaggg ggcgatcacg ccggcgagcg
     4861 cggcgaccac cgtcatcatc acgatgacca cggcgcccag cgcgccgagc gggcggcggc
     4921 gtacgaagtc gcgcgtggcg gcgacccacc gcccctgggt ccgatcttgc gactcggtga
     4981 cggtgtccag ggcgcggtcg gtgacggcca tcaccggtac cggatccgcg gatcgagcca
     5041 ggcgtaggcg atgtcggtca ggaagttcac caggatgaag acgaaggcca cgatcatgac
     5101 gagcgcctgg gtcagcgtgt agtcgcggtg cgcaatggcc tcgacgaaga gcaggccgag
     5161 cccgttcagg ttgaacacct gctcggtgac cacgagaccc ccgaggagga aggcgaactc
     5221 gagaccgatg accgtgagca ccggcagcat ggcgttcttg agcgcgtgcc gcgacaggat
     5281 gagcttgagc cagagcccct tggccctggc cgtccggatg tagtcctcgc gcagcacctc
     5341 gagcatcgcc gaccgcgtca tgcgggtggc gaccgccgag tatcggtagc cgacggcgac
     5401 cgccggccag acgagctggg cgagattctg ccacgggttg acccagaacg gcgtgtacac
     5461 catcgggggt agccacttga agaagaccag ga
//
LOCUS       DATIAZ010000065         4173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1331308, whole genome shotgun sequence.
ACCESSION   DATIAZ010000065 DATIAZ010000000
VERSION     DATIAZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331308"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..882
                     /locus_tag="VLF19_02195"
     CDS             136..882
                     /locus_tag="VLF19_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSE92090.1"
                     /translation="MLDFERHGAVGWLWLNRPQALNALNTALTAALEDALERVAGMDD
                     LSVLVVAGRGRAFCAGNDIAEMGPITPEAAVQLARRQAVLMDRFARLPQVTLAAVDGY
                     ALGGGLMLTVAQDLRIASDRSRFGLPEVTLGFNPAYGISRLLDVAGGAHGRDLLLTGR
                     TVHASEALRMGLVNRVVAAATLHETAATWAAEIAKSPREGLAATKTIVRQLRAGAAGG
                     GEPEAYGAALRTSPAARDRIRAFTDRRRKE"
     gene            complement(920..3145)
                     /locus_tag="VLF19_02200"
     CDS             complement(920..3145)
                     /locus_tag="VLF19_02200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSE92091.1"
                     /translation="MRFWGTRGSIASPGPATAQYGGNTSCVEVRGDDGTVIVLDCGTG
                     ARGLGIDLVRRGGALCVNLFIGHTHWDHIQGFPFFVPAFVEGVDLNIFAPTGFQRGLE
                     EAMAGQMEYSYFPVKLRDLRSTLHFTELEEGFFRVGDLLVETQYLNHTAPTIAYKISG
                     AGGTLAYVTDHEPFWPGRGPEFEHPGDQGHVAFLRGADLVIHDAQYTEEEYPSRVGWG
                     HSTIEYAIAVARAAGVRRLALFHHDPSRDDAEMQRLEARARGGAIGEVEVFAGREGLE
                     LEVRGAAGVPSVASASALSRRPIAGGRVLVVSPNDAEVAAIEELLAIDDLEITAVVNG
                     RAALARCSELEPDVAIVDARLPDGDGVALAAQLAATSGRAVPVLILAEQGMASPTNAA
                     GTADWLAKPFSPPMLHTRVRAWLSRSLAAAAPPDVRPAAAREASVPGHAIAVTADHAA
                     LLGDMPLFRALSGEERERLVDGATEHAFGPGQVIIGEGEVDDRVFVVLAGRVRIIESM
                     PEALSEAVLGGLGEGEVFGELSILTGRPRSATVLAVERTRCLAIHRDRFTQALESYPG
                     LAVGLLRVLARRLIEADRRLARYAPDALTGLASRRALQDQYRRMAAAARRRKTGVLLL
                     VFDVLRLRGINDRFGYVTGDEVLRTVADGLREVTRLTDLVARCGADEFAVLALDAGLR
                     EAELLITRIGERLKTLTAQRVPAAIVQLSMGIASREDAPDSLDELFRAADKDMRRGRV
                     I"
     gene            3278..>4173
                     /locus_tag="VLF19_02205"
     CDS             3278..>4173
                     /locus_tag="VLF19_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HSE92092.1"
                     /translation="MPALPLIPSTVVGSHGKPGWWFALVRAWEAGEAGPGDLDEMLDD
                     AADTAIRDMERAGIDVITDGEVRRLDGYVDSYYAVIKGIAPLPVRRKLGPWGYDQQTR
                     YEAVGRIEPPAGGLGIVKEFSYLKGRTTRRTKATCAGPLTFGSRIHPGTSYRGVVDVA
                     ERFAEVINEELKGLVAAGADWIQIDEPARGNVSGEEMARLFNLATDGVNARLGFHICF
                     GNRFGRSRFPRSYEPYFPGALKARADQFVLEFAGRELAEVDLWKTYGDGRELGAGVVD
                     VKGFNQDTGEDVARRIRRVLQV"
BASE COUNT          612 a         1505 c         1489 g          567 t
ORIGIN      
        1 ggcgcccgct ccgatggccg gcggcaggcg ctccggtgag tggcgcggcg ccgatgctcc
       61 acgtcgatca tccgcgcgag atcgcgccgc gctggtgaac ggtcgcgatc ggcccggcga
      121 gagcgcgtgg cgctgatgct cgacttcgag cgccacggcg cggtggggtg gctgtggctc
      181 aatcgtccgc aagccctcaa cgcgctgaac accgcgctca ccgctgcact cgaggacgcg
      241 ctggagcgcg tggccggcat ggacgacctg agcgtgctcg tcgtcgccgg ccgtggccgc
      301 gccttctgcg ccggcaacga catcgccgag atgggcccga tcacgcccga ggcggccgtg
      361 caactcgccc gccgccaggc cgtgctgatg gaccgcttcg cacgcctgcc ccaggtgacg
      421 ctggccgccg tcgacggcta cgccctcggc ggcggcctca tgctcaccgt cgcccaggac
      481 ctgcggatcg cctcggaccg ctcgcgcttc ggcctgcccg aggtgacgct cggcttcaac
      541 cccgcctatg gcatctcgcg cctgctcgac gtggccggcg gggcccacgg ccgcgacctg
      601 ctgctgaccg gccgcaccgt gcacgcctcg gaggcgctgc gcatggggtt ggtgaaccgc
      661 gtcgtcgccg ccgcgacgct gcacgaaaca gccgccacct gggcggccga gatcgcgaaa
      721 tcgccccgcg aggggctggc cgcgacgaag accatcgtgc gccagcttcg ggcgggcgcg
      781 gccggcggcg gcgagcccga agcctacggc gccgcgcttc gcaccagccc cgccgcccgc
      841 gaccgcatcc gcgccttcac cgaccgcagg cgcaaggaat aacagtccgg ctttgccggc
      901 gtcgaggtcc ccgtccgact cagatcaccc ggccccggcg catgtccttg tcggccgcgc
      961 ggaacagctc gtcgaggctg tccggcgcat cctcgcgaga ggcgatgccc atgctgagct
     1021 ggacgatggc ggcgggcacc cgctgggcgg tgagcgtctt gaggcgctcg ccgatgcggg
     1081 tgatgaggag ctcggcctcg cgcagaccgg cgtcgagcgc gagcacggcg aactcgtcgg
     1141 cgccacagcg cgccaccagg tcggtgaggc gcgtcacctc gcgcagcccg tcggccaccg
     1201 tgcgcagcac ctcgtcgccg gtgacgtagc cgaagcggtc gttgatcccg cgaagccgca
     1261 gcacgtcgaa cacgagcagc agcaccccgg tcttgcgccg gcgggcggcg gctgccatgc
     1321 gccggtactg gtcctggagc gcgcgacggc tcgccagccc cgtcagcgcg tcgggggcat
     1381 agcgggcgag gcgccggtcg gcctcgatga ggcgccgggc gagcacccgc agcagtccga
     1441 ccgcgagccc agggtacgac tccagcgcct gcgtgaagcg gtcgcggtgg atggccaggc
     1501 agcgcgtgcg ctcgacggcc agcacggtgg ccgagcgggg gcggccggtg aggatcgaca
     1561 gctctccgaa gacctcgccc tcccctagcc cacccaaaac ggcctcgctc agggcctccg
     1621 gcatcgactc gatgatgcgc acgcggccgg cgagcacgac gaagacgcgg tcgtccacct
     1681 cgccctcgcc gatgatcacc tgacccggac cgaaggcgtg ctcggtcgcg ccatcgacga
     1741 gccgctcccg ctcctctccc gagagcgcgc ggaacagcgg catgtcgccc agcagggccg
     1801 cgtggtccgc cgtcacggca atcgcgtggc ccggtaccga cgcctcgcgc gcggcggccg
     1861 gccgcacgtc cggcggcgcg gcggcggcga gcgagcgcga gagccacgcg cgcacgcgcg
     1921 tgtgcaacat gggcgggctg aagggcttgg cgagccagtc ggcggtgccg gcggcgttgg
     1981 tgggggacgc catcccctgc tcggcgagta tcagcaccgg cacggctcgc ccggaggtgg
     2041 cggcgagctg agcggccagc gcgacgccgt cgccgtcggg gaggcgggca tcgacgatgg
     2101 cgacgtcggg ctccagctcg gagcagcggg ccagcgccgc acgaccgttc acgaccgcgg
     2161 tgatctcgag atcgtcgatg gccaggagct cttcgatggc cgcgacttcg gcgtcgttcg
     2221 ggctcaccac cagcacgcgt ccccccgcga tcggccgtcg gctcagcgcc gaggccgagg
     2281 ccacggaggg cacgcccgcg gcgccgcgca cctccagctc gagaccctcg cggcccgcaa
     2341 acacctcgac ctcaccgatc gccccgcccc gggctcgcgc ctcgagccgt tgcatctccg
     2401 cgtcgtcgcg gctcgggtcg tggtggaaca gggcgagccg gcggacgccc gcggcccgcg
     2461 cgacggcgat ggcgtattcg atggtgctgt gcccccagcc gacgcggctc ggatactctt
     2521 cctccgtgta ctgcgcgtcg tggatgacca gatcggcgcc cctcaagaat gcgacgtggc
     2581 cctgatcgcc cgggtgctcg aactcggggc cacgtcccgg ccagaacggc tcgtggtcgg
     2641 tcacgtacgc cagggtgccg cccgcgcccg agatcttgta ggcgatcgtg ggcgccgtgt
     2701 ggttcaggta ctgcgtctcc acgagcaggt cgcccacgcg gaagaaccct tcctccagct
     2761 ctgtgaagtg cagcgtcgag cgcagatcgc gcagcttcac cgggaagtag gagtactcca
     2821 tctgcccggc catggcctcc tcgagtccgc gctggaagcc cgtgggagcg aagatgttga
     2881 ggtccacgcc ctcgacgaag gcgggcacga agaacgggaa gccctggatg tggtcccagt
     2941 gggtgtggcc gatgaagaga ttgacgcaca acgcgccgcc gcgtcggacg aggtcgatcc
     3001 ccagtccgcg cgcgccggta ccgcaatcga gcacgatcac ggtgccgtcg tcgcctcgaa
     3061 cctcgacgca cgacgtgttg ccgccgtact gcgccgtggc cggcccgggc gacgcgatgg
     3121 agccgcgagt gccccagaac ctcacgtaca cgccgcgtca cctcgatggg gcgaggtctg
     3181 atccaaaaaa agtgacggca tcatagcacg cacggcccag gagctagcca ccggccgccg
     3241 aacgttctag tctttacccc ccgggaggtg ccgaccgatg ccagcgttgc ccctgatccc
     3301 cagcacagtc gtgggctcgc acggaaaacc cggatggtgg ttcgccctgg tcagggcctg
     3361 ggaggccggc gaggcggggc ctggcgacct cgacgagatg ctcgacgatg ccgccgacac
     3421 tgccatccgg gacatggagc gggccgggat cgacgtcatc accgacggcg aagtgcgccg
     3481 gctcgacggc tatgtcgact cgtactacgc cgtcatcaaa ggcatcgcgc cgctgccggt
     3541 ccgccgcaag ctcggcccgt gggggtacga tcagcagact cgctacgagg ccgtcgggcg
     3601 gatcgagccc ccggccggag ggctcgggat cgtcaaggaa ttctcctatc tgaagggacg
     3661 tacgacccga cgcacgaagg ccacgtgtgc cgggccgctg accttcggct ctcggattca
     3721 tcccggcacg agctaccggg gcgtggtcga tgtggcggaa cgcttcgccg aggtcatcaa
     3781 cgaggagctg aaggggctgg tcgcggcggg cgccgactgg atccagatcg acgagcccgc
     3841 ccggggcaac gtctcgggcg aggagatggc ccgcctcttc aacctggcca cggacggggt
     3901 gaacgcccgc ctggggttcc acatctgttt tggcaaccgc ttcggccgct cgcgcttccc
     3961 gcgctcgtat gagccatact ttccgggcgc gctgaaggca cgcgccgacc agttcgtgct
     4021 cgagttcgcc ggccgcgagc tggccgaggt ggacctctgg aaaacgtacg gcgacgggcg
     4081 ggagctcggt gctggcgtcg tcgacgtcaa gggcttcaat caggacaccg gcgaggatgt
     4141 ggctcgtcgc attcgccgcg tgctccaagt gtg
//
LOCUS       DATIAZ010000066         5261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1067662, whole genome shotgun sequence.
ACCESSION   DATIAZ010000066 DATIAZ010000000
VERSION     DATIAZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067662"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VLF19_02210"
     CDS             complement(<1..360)
                     /locus_tag="VLF19_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HSE92093.1"
                     /translation="MNTHRATTFENRHQAGRALADQLAGVIDPPRTVVAALPRGGVAV
                     ALPIVQRFGVPLAIVYARKLTVPIAPELAVGALDEDGYFLTDPEVVATLGVTPQDVER
                     ARGRVAREIERRMRLYGT"
     gene            complement(357..2768)
                     /locus_tag="VLF19_02215"
     CDS             complement(357..2768)
                     /locus_tag="VLF19_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92094.1"
                     /translation="MTGPGASIAAAVRRLKPEELRAVCEPATLPFASTETLPPLDSML
                     GQDRALGATTFGIRMKHRGYNLFALGPSATGKTTTMRRLLDEAAAAEPVPSDWCYVHN
                     FVDASRPRALEVPAGRGRELREALERFVEECKVRLPRAFESEEFERRKSEILEELARQ
                     QSEEIAKVESAAREAGFVLVRTPGGMAVAPAPEGRPLNPEQFEALPEPVRDRLGAALG
                     PLESRLEVSLRQIRQFERAARAAHEALVRDIAAAATRALVQELRDDFAGIDAVRRYLD
                     QVEEDLVAHAEDLRRGDDHQPALPFLPPPAAFMDRYRVNVLVDRADARGAPVVFERNP
                     THGNLLGRVEHRPQFGTLVTDFTLIKGGALHRANGGYLVLDAKDVLRTFMAWESLKKA
                     LKARAIRIEEPLEELRLISTVTLAPEPIPLDVKVVLIGNPLLYYLLYELDEDFRELFK
                     VKVDFDDSLPRTAESEALYGRFGGTACREEGLRHFSAGGLARLVEHGSRLISHQGRLS
                     ARLGLLLDVVREASFCAEQGGRALVEAQDVTRAIEQRIYRASLLEERAGRLIAEGTQL
                     VDTEGEAVGQVNGISVLVTGDHAFGRPSRITARTYAGAPGVVDIEREAKLGGRVHSKG
                     VMILTGFLAGRFASDRPLALSASLAFEQQYEEIDGDSASSAELYALLSSLAGVPVSQA
                     IAVTGSINQQGEIQPVGGVNEKIEGFFDVCRARGLTGRQGVVIPAANVRHLMLRPAVV
                     EAVRAERFHVWAVTTVDEGIELLTGRPAASVYAAVEQALARNVERLRQLRTEAPTVAR
                     KEDGT"
     gene            complement(2765..3325)
                     /locus_tag="VLF19_02220"
     CDS             complement(2765..3325)
                     /locus_tag="VLF19_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSE92095.1"
                     /translation="MRHPKRSISRDIRTRRPLHPGREADELEAVTWPEGLRVKQFMTR
                     SPVTIHPDALVKGAADMMRTRKLRHLPVVDRGGRLIGIVTDRDLRQVIFDPAVQARLG
                     RAADALQTLAVRDVMTWGVVTVRPETTVRDAAWLMREQRLGALPVVQAGRLVGILTER
                     DVLRAFEEALGTDALTRPYRWAFSYR"
     gene            complement(3322..4767)
                     /locus_tag="VLF19_02225"
     CDS             complement(3322..4767)
                     /locus_tag="VLF19_02225"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSE92096.1"
                     /translation="MKTVPIGDCIWEIPPSEKAGMLVPARLYASERLLARMDAGVFEQ
                     VTNVACLPGIVRAALCMPDGHWGYGFPIGGVAAFRTDTGVISPGGIGFDINCGMRVLR
                     TTLTEADVRPRLRELVDALFAAVPTGVGSRGFVHLTDDMFREVMVEGAGWCVRHGYGW
                     PEDLAATEGGGCLPGADPAHVSARALQRGRSQLGTLGSGNHYLEIQVVPPDGIHERHV
                     ARAFGIDRPGQVLVMLHCGSRGFGHQVGTDYLRVFDQAMPRYGITVRDRELACAPFRA
                     PEAQAYFTAMNAAANTAFANRQVITHRVREVLSRVFGREARALGLDVIYDVCHNTAKL
                     ERHVVDGEALELCVHRKGATRSFGPGAPDVPEAYREVGQPVIIGGSMETGSALLVGTR
                     HAMQETFGSTAHGAGRTMSRAQAKRQVWGETLIRDMEARGVVVRAASKSGVAEEAGFA
                     YKDLDEVVDVVHRLDVSRKVVSLKPMGNIKG"
     gene            complement(4770..>5261)
                     /locus_tag="VLF19_02230"
     CDS             complement(4770..>5261)
                     /locus_tag="VLF19_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HSE92097.1"
                     /translation="GLQGMTGGSEVAPIGDPAGGPVGRYEVLEAMAVADCALDVEGES
                     LDDLFETAARAVAELMVDPATVSPSAEREVALTASSLDLLLYDWLSELIFLKDSEQMV
                     FPETTVRVSGPSPARLGARLRGAVIDPARMTLRADPKAVTFHQFALAPHDGGWRARLV
                     IDI"
BASE COUNT          728 a         1964 c         1857 g          712 t
ORIGIN      
        1 cgtcccgtac agccgcatcc gccgctcgat ctcccgggcg acgcgaccgc gcgcccgctc
       61 gacgtcctgc ggcgtgacac cgagcgtggc gaccacctcc ggatcggtca gaaaataccc
      121 gtcctcgtcg agcgcgccca cggccagctc cggcgcgatc ggcaccgtca gcttgcgcgc
      181 atagacgatg gccagcggga cgccgaaccg ctggacgatc ggcagggcca cggcgacgcc
      241 gccgcggggg agggcggcga cgaccgtccg gggcggatcg atgacgccgg cgagctggtc
      301 tgccagagcg cggcccgcct ggtgccggtt ctcgaacgtg gtcgcgcggt gggtgttcac
      361 gtcccgtcct ccttccgcgc caccgtcggc gcctcggtcc ggagctgtcg cagccgttcc
      421 acgttgcgcg ccagggcttg ctcgacggcg gcatacacgc tggcggccgg gcgcccggtg
      481 agcagctcga tgccctcgtc caccgtcgtg acggcccaga cgtggaagcg ctcggcgcgc
      541 acggcctcga cgacggcggg acgcagcatc aggtggcgca cgttcgcggc ggggatcacg
      601 accccctggc gccccgtcag cccgcgcgcg cggcagacgt cgaagaatcc ctcgatcttc
      661 tcgttgacgc cgccgaccgg ctggatctct ccctgctggt tgatggaccc cgtcaccgcg
      721 atcgcctgcg acaccgggac gccggccagg ctcgacagca gcgcgtacag ctccgccgac
      781 gaggcgctgt ccccgtcgat ctcctcgtac tgctgctcga aggcgagcga cgcggacagc
      841 gcgagcgggc ggtcgctcgc gaagcggccg gcgaggaacc cggtgaggat catgacgccc
      901 ttggagtgga cgcgcccgcc gagcttcgcc tcgcgctcga tgtccacgac gcccggcgcg
      961 cccgcgtacg tccgcgcggt gatccgggag ggccgtccga aggcgtggtc cccggtcacg
     1021 agcaccgaga tgccgttgac ctggcccacg gcctcgccct cggtgtcgac gagctgcgtg
     1081 ccctcggcga tgaggcggcc ggcgcgctcc tcgagcaggc tcgcgcggta gatgcgctgc
     1141 tcgatcgccc gcgtgacgtc ctgcgcctcc acgagcgctc gcccgccctg ctcggcgcag
     1201 aacgacgcct cgcgtacgac gtccagcaac agtccgagcc gcgcgctgag ccggccctgg
     1261 tgcgagatga ggcgggagcc gtgctcgacg agccgcgcga gcccccccgc cgagaagtgc
     1321 cgcaggccct cctcgcggca cgccgtgccg ccgaaccggc cgtagagcgc ctcggactcc
     1381 gcggtgcgag gcagcgagtc gtcgaagtcc acctttacct tgaagagctc gcggaagtcc
     1441 tcgtcgagct cgtagaggag gtagtagagc agcgggttgc cgatgagcac gaccttcacg
     1501 tcgagcggga tcggctccgg cgccagggtc accgtgctga tgaggcgcag ctcctccagc
     1561 ggctcctcga tgcggatcgc gcgggccttg agggccttct tgagcgactc ccacgccatg
     1621 aaggtgcgca ggacgtcctt ggcgtcgagg accaggtagc ccccgttcgc gcggtgcagc
     1681 gcgccgccct tgatgagcgt gaagtcggtg acgagcgtgc cgaattgcgg gcggtgctcg
     1741 acccgcccca gcaggttgcc gtgcgtcggg ttccgctcga agaccaccgg cgcgccgcgc
     1801 gcgtccgcgc ggtcgacgag cacgttgacg cggtagcggt ccatgaaggc ggccggcggg
     1861 ggcaggaacg gcagcgccgg ctggtgatcg tctccccggc ggaggtcctc ggcgtgcgcc
     1921 acgaggtcct cttccacctg gtcgaggtag cggcgcacgg cgtcgatccc ggcgaagtcg
     1981 tcgcggagct cctgcaccag cgcgcgggtc gccgcggcag cgatgtcacg gaccagcgcc
     2041 tcgtgggccg cgcgggccgc gcgctcgaac tggcggatct gacgcagcga cacctccagg
     2101 cgcgactcga gcggccccag ggcagccccc agccggtcgc gcaccggctc gggcagcgcc
     2161 tcgaactgct cgggattcag cggacggccc tccggcgcgg gcgccaccgc catcccgccg
     2221 ggcgtccgca ccagcacgaa gcccgcctcg cgggccgccg actcgacctt cgcgatctcc
     2281 tccgactgct ggcgcgccag ctcctcgagg atctccgact tgcggcgctc gaactcctcg
     2341 ctctcgaagg cgcggggcag ccgcaccttg cactcctcga cgaagcgctc cagcgcctcg
     2401 cgcagctcgc gcccacggcc cgccggcacc tccagcgcgc gcgggcggga ggcgtcgacg
     2461 aagttgtgga cgtagcacca gtccgacggc accggctccg cggccgccgc ctcgtcgagc
     2521 aggcggcgca tcgtggtcgt cttgccggtg gccgacggcc cgagcgcgaa caggttgtag
     2581 ccccggtgct tcatgcggat gccgaacgtc gttgcgccga gcgcgcggtc ctggccgagc
     2641 atgctatcga gcggcggcag cgtctccgtc gaggcgaacg gcagcgtcgc cggctcgcag
     2701 acggcccgca actcctccgg cttcagtcgc cggacggcgg cggcgatgga cgcgccgggc
     2761 ccggtcatcg gtacgagaac gcccagcggt agggacgcgt cagggcatcg gtgccgagcg
     2821 cctcctcgaa ggcccgcagc acgtcgcgct cggtgaggat gccaaccagg cggccggcct
     2881 ggaccaccgg cagcgcgccg aggcgctgct cgcgcatcag ccaggccgcg tcgcggacgg
     2941 tggtctcggg acgcacggtc acgacgcccc aggtcatgac gtcgcgaacc gcgagggtct
     3001 gcagcgcatc ggccgcccgc ccgaggcgcg cctggaccgc cggatcgaag atcacctggc
     3061 gaagatcgcg gtccgtgacg atgccgatca ggcgcccgcc ccggtcgacc acgggcaggt
     3121 gacgcagctt ccgcgtccgc atcatgtccg ccgcgccctt cacgagcgcg tcggggtgaa
     3181 tcgtgaccgg cgagcgcgtc atgaactgct tgacgcgcag gccctcgggc caggtgaccg
     3241 cctccagctc gtcggcctcg cgtccgggat gcagcgggcg gcgggtacgg atgtcgcggc
     3301 tgatcgatcg cttcgggtgt ctcatccctt tatgttcccc atgggcttca ggctgacgac
     3361 cttgcgggag acgtcgagcc ggtgcacgac gtccacgacc tcgtccaggt ccttgtaggc
     3421 gaaccccgcc tcctccgcca cgccggactt cgacgcggcg cggaccacca cgccgcgggc
     3481 ctccatgtcc ctgatcagcg tctcgcccca cacctggcgc ttggcctgcg cgcgcgacat
     3541 cgtccggccg gcgccgtgcg ccgtggagcc gaacgtctcc tgcatcgcgt ggcgcgtgcc
     3601 gacgagcagc gccgatcccg tctccatcga gccgccgatg atcaccggct gccccacctc
     3661 gcggtacgcc tccggcacgt cgggcgcgcc cggtccgaac gagcgcgtgg cgcccttgcg
     3721 gtggacgcag agctccagcg cctcgccgtc caccacgtga cgctcgagct tcgcggtgtt
     3781 gtgacagacg tcgtagatca cgtccaggcc cagcgcgcgg gcctcgcgcc cgaacacacg
     3841 cgacagcacc tcgcgcaccc ggtgggtgat gacctggcgg ttggcgaacg ccgtgttggc
     3901 ggcggcgttc atggcggtga agtacgcctg cgcctcgggc gcgcggaagg gcgcacaggc
     3961 cagctcgcgg tcacggaccg tgatgccgta gcgcggcatc gcctggtcga agacgcgcag
     4021 gtagtcggtc cccacctggt ggccgaaccc ccgcgagccg cagtgcagca tgacgagtac
     4081 ctgtccggga cggtcgatgc cgaaggcccg cgccacgtgc cgctcgtgga tgccgtcggg
     4141 gggcacgacc tggatctcca ggtagtggtt gccggagccg agcgtgccga gctggctgcg
     4201 cccgcgctgc agcgcccgcg cgctgacgtg cgccgggtcg gcgccgggca gacagccgcc
     4261 gccctccgtc gccgccaggt cctccggcca gccgtacccg tggcgcacgc accagccggc
     4321 gccctcgacc atcacctcgc ggaacatgtc gtccgtgagg tgcacgaacc cgcgcgagcc
     4381 cacgccggtc gggacggcgg cgaagagtgc atcgaccagc tcgcgcagcc gcggacgcac
     4441 gtcggcctcg gtcagggtcg tgcgcagcac gcgcatgccg cagttgatgt cgaagccgat
     4501 gccgcccggc gagatcacgc cggtgtccgt gcggaaggcc gccacgccgc cgatgggaaa
     4561 gccgtagccc cagtggccat ccggcatgca gagcgcggcg cgcacgatgc ccggcaggca
     4621 ggcgacgttg gtgacctgct cgaagacgcc ggcgtccatg cgcgcgagca ggcgctccga
     4681 ggcgtagagg cgcgcgggca ccagcatccc ggccttctcg ctcggcggga tctcccagat
     4741 acagtcgccg atcgggaccg tcttcatgcc tatatgtcga tgaccaggcg cgcccgccag
     4801 ccgccgtcgt gtggggcgag cgcgaactga tggaaggtga cggccttggg atccgcgcgc
     4861 agcgtcatgc gcgccggatc gatgacggcc ccgcgcagcc gggcgccgag ccgcgcgggc
     4921 gacgggccgc tgacccggac cgtggtctcg gggaacacca tctgctcgct gtccttcagg
     4981 aagatcagct ccgagagcca gtcgtagagc agcagatcga gcgaggacgc ggtcagcgcc
     5041 acctcgcgct ccgcgctcgg cgacaccgtc gccgggtcca ccatcagctc cgcgacggcg
     5101 cgcgcggcgg tctcgaacag gtcgtccagg ctctcgccct cgacgtcgag cgcgcaatcg
     5161 gccaccgcca tggcttcgag cacttcgtag cgtcccacag gccctcccgc cgggtcgccg
     5221 atcggtgcaa cctcactgcc acccgtcatt ccttgcaatc c
//
LOCUS       DATIAZ010000067         2735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1594612, whole genome shotgun sequence.
ACCESSION   DATIAZ010000067 DATIAZ010000000
VERSION     DATIAZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594612"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1605)
                     /locus_tag="VLF19_02235"
     CDS             complement(100..1605)
                     /locus_tag="VLF19_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92098.1"
                     /translation="MSATRIASLWVPWFAAAAATRSEPALADRPLVIVRGLPPVARVL
                     EAGAAARERGVAPGMTETEARGRCPGLVSRPWREEPMSSARHALLEAALAVSPRLEDG
                     GPGLVFADLAGLGRLFGDDDAIAGYLARLARRVGLPGRVAVAGTRTTARIVARSAATP
                     VTVVPPGGDRAALARTRVDALDLDAAVAAALARWGVTTLGELAALPRAGLVTRLGPAG
                     LAAHGAALGRDTGPFTPWTPPPFWEEAQGLDWEIDELEALVTVLGGVLERLAERLTAA
                     HVWADTLDLGLELATGARHERGITLAHPTREVPLLRMLVRHDLAAHPPPAAVTGVAVS
                     VRAVRAEAGQAGLWRPPAPLGRDLAGVVARLTALVGGDNLGSPALGDSYRPDHVALIP
                     FVPPDDDAPPATDDPGPPDDAAPPIVLRRLRPPRAVEVTPGPDGRPAVVRRGDRDEHV
                     VACAGPWRASGEWWDTRGWAREEWDARLHDGTLCRLAREQTGRWLLDGVYD"
     gene            complement(1602..2195)
                     /locus_tag="VLF19_02240"
     CDS             complement(1602..2195)
                     /locus_tag="VLF19_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92099.1"
                     /translation="MIVLRGEITEIVGALSSGRSSVLVAGLRDVTRAGAVAALVDVDH
                     AFDPASAAAAGVDLRRLLWVRCDGRRDAALRAADLLTRCPGFALVALDLGESPPRLPL
                     TAAFRLRLAARGSGTALVVLARQRIAGTAAALALETSRGTVQWAGPGRAPTRLARVET
                     RLRVIRRRGGVEAVVGAAGPTTVAPARPGEVSEEASP"
     gene            2364..>2735
                     /locus_tag="VLF19_02245"
     CDS             2364..>2735
                     /locus_tag="VLF19_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSE92100.1"
                     /translation="MAKQIGYALVGLGTLARRFVLPAFAQSTEHSRLAAVVSGDRAKA
                     QLVAQQFHAAAYHYDELRQCLQRDDVHALYIALPNSLHCDYAVEAARAGVHVLCEPPM
                     AVTADECRRMIRTCQTNRVKMM"
BASE COUNT          382 a         1015 c         1020 g          318 t
ORIGIN      
        1 tgccgcttcc tcgaccaggg tctcgggttg ctcgacggct tcgaggaacg agaacgcgga
       61 ctgcgcgtgc agctcgacga acatctcatg cgccttgcat cagtcgtaga cgccgtcgag
      121 gagccaccgg ccggtctgtt cgcgggcgag acggcagagc gtgccgtcgt ggagccgcgc
      181 gtcccattcc tcgcgcgccc agccgcgcgt gtcccaccac tcgcccgagg ccctccatgg
      241 gccggcgcac gccaccacgt gctcgtcacg gtcgccccgc cgtaccacgg cgggacgtcc
      301 gtcggggccg ggcgtcacct cgacggcccg cggcgggcgc agccggcgca ggacgatcgg
      361 cggggcggcg tcatcgggcg gtccggggtc gtccgttgcg ggcggcgcat cgtcgtcggg
      421 aggaacgaac gggatcagag cgacgtggtc ggggcgatac gagtcgccga gcgcggggga
      481 gccgaggttg tcgccgccca cgagcgcggt gaggcgcgcg acgaccccgg cgaggtcgcg
      541 cccgagcggc gccggcggcc gccagaggcc ggcctgcccg gcctcggcgc gcacggcgcg
      601 gacgctcacc gcgacgccgg tgacggccgc gggcggcgga tgcgcagcga ggtcgtgccg
      661 cacgagcatg cgcagcagcg ggacctcgcg tgtgggatgc gccagcgtga tgccgcgctc
      721 gtggcgcgcg ccggtcgcca gttcgagccc gaggtcgagc gtgtcggccc acacgtgcgc
      781 ggcggtgagc cgctcggcca gacgctccag cacaccgccg agcacggtca cgagcgcctc
      841 gagctcgtcg atctcccagt cgagcccctg cgcctcctcc cagaacggcg gcggggtcca
      901 cggggtgaac ggcccggtgt cgcgtccgag cgcggcgccg tgcgcggcga gacccgcggg
      961 cccgagccgg gtgaccagcc ccgcgcgcgg tagcgccgcc agctcgccga gtgtggtcac
     1021 gccccagcgg gcgagcgcgg ccgcgactgc ggcgtcgagg tcgagcgcgt cgacgcgcgt
     1081 gcgtgccagt gcggcgcggt cgccgccggg cgggacgacg gtgaccggcg tcgccgcgct
     1141 gcgtgcgacg atgcgcgccg ttgtccgggt gccggccacg gcgacgcgcc ccggcaaccc
     1201 cacacgacgc gccagtcggg ccaggtaccc ggcgatggcg tcgtcgtcgc cgaagagccg
     1261 gccgagcccg gccaggtcgg cgaagaccag gcccggcccg ccgtcctcga ggcggggcga
     1321 cacggcgagc gcggcctcga gcagcgcgtg gcgcgccgag gacatcggct cctctcgcca
     1381 cgggcggctc acgaggccag ggcagcgccc ccgcgcctcg gtctccgtca tgcccggcgc
     1441 cacgccacgc tcgcgcgccg ccgcgccggc ctcgaggaca cgcgccacgg gcggtagtcc
     1501 gcgcacgatc acgagcgggc ggtcggcgag cgctggctcg gagcgagtgg ccgcggccgc
     1561 ggcgaaccac ggcacccaca gcgacgcgat gcgggtcgcg ctcatggtga cgcctcttcc
     1621 gacacctccc cgggtcgtgc cggggcaacc gtcgtcggcc cggcggctcc caccacggct
     1681 tcgacgccgc cacgccggcg gatcacgcgc aggcgcgtct ccacgcgcgc cagacgcgtt
     1741 ggagcacggc cgggccctgc ccactgcacc gtccctcgcg acgtttccag ggcgagggcc
     1801 gcggcggtgc cggcgatgcg ctgacgcgcg aggacgacga gcgcagtgcc ggaaccccgc
     1861 gcggcgagcc gcagccggaa cgccgccgtc aacggcagcc gtggcggcga ttcgcccaga
     1921 tcgagcgcca cgagcgcaaa gcccggacag cgtgtaagga ggtcggcggc gcgcagcgcc
     1981 gcatcacggc gcccgtcgca gcgtacccag agcagccgcc gcagatcgac cccggccgcc
     2041 gccgcggagg cgggatcgaa ggcgtggtcg acgtccacca gggcggcgac ggcgccggcg
     2101 cgcgtcacgt cgcgcaagcc ggcgacgagc acgctcgagc gtcctgacga gagcgccccg
     2161 acgatctccg tgatctcgcc gcgaagcacg atcattctca ttgcttatca cgtgttgagt
     2221 ggagagcaag gcctcagatg ttgggggagg gacgtgtggg ggccctacat acaggggatt
     2281 cactcaccac cctgaaacgg aagccacacg acatggcctc gcccttgcac ccttgctcga
     2341 gcggatcgat atagagattg atcatggcca agcaaatcgg gtacgcgctc gtcgggctgg
     2401 ggacgctcgc ccggcgcttc gtgctccccg cattcgcaca atccaccgag cattcgcgcc
     2461 tggctgccgt cgtgtccggc gaccgcgcca aggcgcagct cgtcgcgcag cagttccacg
     2521 ccgcggcgta ccactacgac gagctgcgcc agtgcctgca acgcgatgat gtgcacgcgc
     2581 tgtacatagc cctgccgaac tctctgcact gcgactacgc ggtggaggcg gcgcgggcgg
     2641 gcgtgcacgt tctctgcgag ccgccgatgg cggtgaccgc cgacgagtgc cgccgcatga
     2701 tccgcacgtg ccagacgaac cgtgtgaaga tgatg
//
LOCUS       DATIAZ010000068         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1594839, whole genome shotgun sequence.
ACCESSION   DATIAZ010000068 DATIAZ010000000
VERSION     DATIAZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594839"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..210
                     /locus_tag="VLF19_02250"
     CDS             37..210
                     /locus_tag="VLF19_02250"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HSE92101.1"
                     /translation="MRLREEIQRTLGTPAKIRWGGFGELTVTVDGRTVFSKRQAGRVP
                     EPGEVARLLGSAR"
     gene            complement(222..2123)
                     /locus_tag="VLF19_02255"
     CDS             complement(222..2123)
                     /locus_tag="VLF19_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HSE92102.1"
                     /translation="MKSAYEQLYARSMRDAEGFWAAAAEDVYWDRRWDRVFDGSGAPF
                     YRWFVGGQLNTCYNCLDLHVDRGRGKQRALVYDSPVTSTVKTFTFDELRDQVARVAGA
                     LRRQGIGKGDRVIIYMPMVPEAVMAMLACARLGAVHSVVFGGFAAHELAKRIDDARPR
                     LIVSASCGIEVNRVVAYKPLLDGAIELASHKPERCVILQRAVETAPLRPGRDLDWDDF
                     MAGAEPAECVPMAATDPLYVLYTSGTTGIPKGVVRDNGGHAVALKWSMQSIYGVGSGE
                     VYWAASDIGWVVGHSYIVYAPLLKGCTTILYEGKPVGTPDPGAFWRVCADHGVNALFT
                     APTAFRAIKKEDPQGAFMAKYDLRHFRTLFLAGERCDPDTLLWARERLGVPVIDHWWQ
                     TETGWPIAANCVGLGMLPVKPGSPTKPVPGYDVRVLGENNEEMAPDQIGSIAIRLPLP
                     PGCLPTLWNNDAGYQSSYLTRHPGHYLTGDAGYKDADGYVYIMSRVDDIINVAGHRLS
                     TGAMEEVIAAHPDVAECAVVGVADDIKGEVPVGFVVTKAGVTRGEADIARELVERIRE
                     RIGPVAAFKTALVVKRLPKTRSGKILRSTMKRIADGQEYAVPATIDDPGILPEIADVL
                     KVVGYPKNV"
BASE COUNT          357 a          926 c          945 g          387 t
ORIGIN      
        1 cggtgcctcg tctgagccga cctccggcgt gaggccgtgc gcctccgcga ggagatccag
       61 cgaacgctgg ggacgcccgc gaagattcgc tggggcgggt tcggggagct gaccgtcacg
      121 gtggacggtc ggaccgtctt ctccaagcgt caggccggcc gcgtgcccga gccgggcgag
      181 gtcgcccggc tcctcggctc cgcgcgctga cgggccgcgg atcacacgtt cttcggatag
      241 ccgacgacct tcagcacgtc ggcgatctcg ggcaggatcc cggggtcgtc gatcgtcgcc
      301 ggcacggcgt actcctggcc gtccgcgatc ctcttcatgg tggatctcag gatcttgccc
      361 gagcgcgtct tgggcagacg cttgacgacc agtgcggtct tgaacgccgc cacgggcccg
      421 atgcgctcgc ggatccgctc gaccagctcc cgggcgatgt cggcctcgcc gcgcgtgacg
      481 ccggccttgg tcaccacgaa gcccaccggc acctcaccct tgatgtcgtc ggcgacgccg
      541 acgaccgcgc actccgccac gtcgggatgc gcggcgatga cttcctccat ggcgcccgtc
      601 gagaggcggt gcccggcgac gttgatgatg tcgtccacgc ggctcatgat gtagacgtac
      661 ccgtcggcgt ccttgtagcc ggcgtcgccg gtcaggtagt ggccgggatg cctggtgagg
      721 tacgacgact ggtagcccgc gtcgttgttc cagagcgtcg gcaggcagcc gggcggcaac
      781 ggcaagcgga tggcgatgga gccgatctgg tcaggcgcca tctcctcgtt gttctcgccg
      841 agcacgcgca cgtcgtagcc gggcacgggt ttggtgggcg acccgggctt gaccggcagc
      901 atcccgagcc ccacgcagtt ggcggcgatc ggccagcccg tctcggtctg ccaccagtgg
      961 tcgatcaccg gcacgccgag ccgctcgcgg gcccacagga gcgtgtcggg atcgcagcgc
     1021 tcgccggcga ggaacagcgt ccggaagtgc cgcagatcgt acttggccat gaacgcgccc
     1081 tgcgggtcct ccttcttgat ggcccggaag gcggtgggcg cggtgaagag cgcgttgacg
     1141 ccgtggtccg cgcacacgcg ccagaacgcg ccggggtccg gcgtgcccac gggcttgccc
     1201 tcgtacagga tcgtcgtgca gcccttcagt agcggcgcgt acacgatgta ggagtggccg
     1261 acgacccagc cgatgtcgga cgccgcccag tacacctcgc ccgatccgac gccgtagata
     1321 ctctgcatcg accacttgag ggccacggcg tgaccgccgt tgtcgcgcac cacgcccttg
     1381 ggaatccccg tggtgccgga cgtgtagagg acgtagagcg gatcggtggc cgccatcggc
     1441 acgcactcgg ccggctcggc accggccatg aagtcgtccc agtcgagatc gcgccctggc
     1501 ctgaggggcg ccgtctccac cgcgcgctgg aggatcacgc agcgctcggg cttgtggctc
     1561 gccagctcga tggcgccgtc gagcagcggc ttgtaggcga cgacgcggtt cacctcgatg
     1621 ccgcaggacg ccgacacgat gagccgcggc cgggcgtcgt cgatgcgctt ggcgagctcg
     1681 tgggcggcga agccaccgaa gacgaccgag tgcacggcgc cgagacgggc gcaggccagc
     1741 atcgccatga cggcctcggg caccatcggc atgtagatga tcacgcggtc gcccttgccg
     1801 atcccctggc gccggagcgc accggcgacg cgcgcgacct ggtcgcgcag ctcgtcgaag
     1861 gtgaacgtct tcacggtgga ggtcaccggg ctgtcgtaga cgagcgcgcg ctgcttgccc
     1921 cgcccccggt cgacgtggag gtcgaggcag ttgtagcagg tgttgagctg accgccgacg
     1981 aaccagcggt agaacggcgc gcccgagccg tcgaagacgc ggtcccagcg ccggtcccag
     2041 tacacgtcct cggccgccgc ggcccagaag ccctccgcgt cgcgcatcga ccgcgcgtag
     2101 agctgctcgt aggcactctt catcgctgcc tcctcgtgga gcgtgcgccc cggcccggcc
     2161 gggcgcaggg cacacgttag ccgcggcgcc ggagccgcgc aagtggccgc ggcggcgcag
     2221 gactggccgc cgctcgtcgg cgaggtccag tgcgtcgccg cgctcgcgct cggacgggcg
     2281 ccgtcaccgg cgcgggacga gccctggcat ctcgctcgcg tgtcgtgcct cgaatgcggc
     2341 gatctcgcgc tcgtggccga gggtccggcg cgtggaccgc gccgtcgggg ggcggtcacg
     2401 gtctgggtct cgaggtccac ggactggtcg gccgtcgcga ctgcgaggtc cggacggcgc
     2461 acccgccgac cggccttcgc gagcctgacg aaggcgtgcg tcgagccgcc gggcctggag
     2521 ccgtggcctg ccgcgccctc ggtgaaccgt caggacgccc cccatactat cgagaggcat
     2581 ggagctcccg ggcggcctgc gcgcgctgaa ccacc
//
LOCUS       DATIAZ010000069         3129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1463199, whole genome shotgun sequence.
ACCESSION   DATIAZ010000069 DATIAZ010000000
VERSION     DATIAZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463199"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VLF19_02260"
     CDS             complement(<1..522)
                     /locus_tag="VLF19_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSE92103.1"
                     /translation="MVHATEREPAKAAGPLTLHRLSVEGVNQCVHCGLCLPYCPTFSE
                     LGTEMDSPRGRIFLIKSLAEGRVALSDSVVDHLSLCLDCRACETACPAGVPYGRLIEA
                     AKAEIERQRPGSLLRRAFRRANFGMLLGHPAALRAAAAALRVYQASGLQRLVRATGLA
                     GLLPGTLPAWEALL"
     gene            complement(528..1871)
                     /locus_tag="VLF19_02265"
     CDS             complement(528..1871)
                     /locus_tag="VLF19_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSE92104.1"
                     /translation="MEASLLDKVRSIVGPAHVLTGVDLSPYVLEGRTPEAAVFPASLD
                     ETRAVVEVAAAAGVPVVPWGGGTAAAVGTPATRAGLVLGLGRLVALVEHEPGDLTVTA
                     QAGMTIAALQAALRARGQWLSLDPPDAGRATVGGVLAANASGPRRHLYGTARDLLIGL
                     TVVTGEGTVVRGGGKVVKNVAGYDLPKLFVGSYGTLGVIVEATLKLRPLPEVERLVAV
                     RFDRVKDAGAAARALAGSDLVPTALDLLDAEAGRALGHGHGGGAPSGPGGAVLVIGFD
                     GLAEQVDWQCGELARLAAPLGGGRVEVLPPTAWDWLATAARRAFEAPAAVMRLVVLPA
                     QVADVMEQAAGAARARGLAAAASAHAGVGVLTVALGAAGSSRESASVAALLHEWRAMA
                     RAGGGHATLEWAPLAVKAEVPVWDDAGAAGRIMQRIKAQLDPQGLFNPGRFIGGI"
     gene            complement(1891..>3129)
                     /locus_tag="VLF19_02270"
     CDS             complement(1891..>3129)
                     /locus_tag="VLF19_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSE92105.1"
                     /translation="TGLSGGCLPAEGGIVISLMRMNRVLEVDYDNQIAVVEPGLVNLH
                     LSWAVGPAGYYYAPDPSSQQACTIGGNIANNSGGPHTLKYGVTVNHVLGLEVVMPDGE
                     IVWLGGKTRESAGYDLPGIFVGSEGTFGVATKIVVRILKKPQAVKTVLAVFDEVAKAS
                     QAVSAIIARGLVPAAVEMIDQLTIQAVEDAFGCGYPRDAAAALLVELDGLAAGMEAQT
                     ERLVAACRDAGAREVRTARDEAERALLWKGRKSAFGAYGRVSPAYMVMDGVIPRTRLP
                     YVLDRVNAIVAAHGLRVGNVFHAGDGNLHPNILYDPRKAGEEARVVAAGAEIMKLCAE
                     VGGSISGEHGIGLEKTDFMPFIFSAADLAFMERLKAAFNPTGRCNPGKVFPTRKTCGE
                     AGPVAYRPHPIEEKGLAQRF"
BASE COUNT          436 a         1187 c         1129 g          377 t
ORIGIN      
        1 cagcaaggcc tcccacgccg gcagcgtccc gggcagcagt cccgccaggc ccgtggcgcg
       61 gacgagccgc tggagcccgc tcgcctggta gacgcgcagc gcggccgccg ccgcgcggag
      121 cgccgcggga tgccccagca gcatcccgaa gttcgcccgg cggaacgcgc ggcggagcag
      181 gctgcccgga cgctggcgct cgatctcagc cttggccgcc tcgatgagcc gcccgtaggg
      241 cacgccggcc gggcacgcgg tctcgcacgc acggcagtcg aggcacagcg acaggtggtc
      301 gaccaccgag tcgctcagcg ccacgcggcc ctcggccagc gacttgatga ggaagatgcg
      361 gccgcgtggc gagtccatct cggtaccgag ctcggagaac gtcgggcagt acggcaggca
      421 cagcccgcag tgcacgcact ggttcacgcc ctccacgctg aggcggtgga gcgtcagtgg
      481 ccccgccgcc ttcgccggct ctcgctccgt cgcgtgcacc atcgcggtca gatgcccccg
      541 atgaaccggc cgggattgaa gaggccctgc gggtcgagct gcgccttgat ccgctgcatg
      601 atgcgcccgg cggccccggc gtcgtcccac acgggcacct cggccttgac cgccagcggc
      661 gcccactcga gcgtggcgtg cccgccgccg gcgcgcgcca tggcccgcca ctcgtggagc
      721 aaggcggcga cggacgccga ttcccgcgag ctgcccgcag caccgagcgc gaccgtgagc
      781 acgccgacgc cggcatgagc gctcgccgcg gcggcgaggc cgcgcgcacg ggccgcgccg
      841 gccgcctgct ccatgacgtc ggcgacctgc gcgggcagca cgacgagacg catcaccgcg
      901 gcgggtgcct cgaaggcgcg gcgcgccgcc gtggccagcc agtcccacgc ggtcggcggc
      961 aggacctcga cgcgcccgcc accgagcggc gcggccaggc gcgccagttc gccgcactgc
     1021 cagtcgacct gctcggcgag tccgtcgaat ccgatgacca ggacggctcc gccggggccg
     1081 gacggcgcgc cgcccccgtg gccgtgaccg agcgcacgcc ccgcctcggc gtccaacagg
     1141 tcgagcgccg tcgggaccag gtccgatccc gccagggcgc gcgccgcggc gcccgcgtcc
     1201 ttcacgcggt cgaagcgcac ggcgacgagg cgctcgacct cgggcagcgg acgcagcttg
     1261 agcgtcgcct cgacgatcac ccccagcgtg ccgtaggagc cgacgaagag cttcggcagg
     1321 tcgtagccgg cgacgttctt caccaccttg cccccgccac gaacgaccgt cccctcgccg
     1381 gtgaccacgg tgaggccgat caggagatcg cgcgcggtgc cgtaaaggtg gcggcgcggc
     1441 cccgacgcgt tcgcggcgag gacgccgcca acggtcgccc gccccgcgtc gggcgggtcg
     1501 agcgacagcc actggccgcg ggcgcgcagc gccgcctgga gcgctgcgat ggtcatgccc
     1561 gcctgcgccg tcaccgtcag gtcgccgggc tcgtgctcga cgagcgcgac gagccggccg
     1621 aggccgagca cgaggcccgc gcgcgtggcc ggcgtgccca cggccgctgc cgtcccgccg
     1681 ccccatggca cgaccggcac ccccgccgcc gcggcgacct ccacgacggc gcgggtctcg
     1741 tcgagagagg ccgggaacac ggcggcttcc ggcgtgcggc cctccagcac gtagggcgac
     1801 aggtccacgc cggtgaggac gtgggcgggc ccgacgatgg agcgcacctt gtcgagcagc
     1861 gaagcctcca cggcagaggg cgcacccgcg ctagaagcgc tgggcgaggc ccttctcttc
     1921 gatgggatgc ggccggtagg cgacgggccc ggcctcaccg catgtcttgc gggtggggaa
     1981 caccttgccg gggttgcagc ggcccgtggg gttgaacgcc gccttgagcc gctccatgaa
     2041 cgcgaggtcc gccgccgaga agatgaacgg catgaagtcg gtcttctcga gcccgatgcc
     2101 gtgctcaccc gagatcgagc ccccgacctc ggcgcagagc ttcatgatct cggcgcccgc
     2161 cgccaccacg cgcgcctcct cgcccgcctt gcgcgggtcg tagaggatgt tcgggtgcag
     2221 gttgccgtcg ccggcgtgga acacgttgcc cacgcgcagg ccgtgagcgg cgacgatggc
     2281 gttcacgcgg tcgagcacgt acggcagccg cgtccggggg atcacgccgt ccatcaccat
     2341 gtacgcgggc gacacgcgcc cgtaagcccc gaacgcggac ttgcggccct tccagagcag
     2401 cgcacgctcg gcctcgtcgc gcgccgtccg gacctcgcgg gcgccggcgt cccggcaggc
     2461 agccacgagg cgctcggtct gcgcctccat cccggcggcg agcccgtcca gctcgacgag
     2521 cagcgcggcg gcggcgtcgc gcgggtagcc gcagccgaag gcgtcctcca ccgcctggat
     2581 cgtgagctgg tcgatcatct cgacagcggc gggcacgagc cctcgcgcga tgatggccga
     2641 caccgcctgg gaggccttgg cgacctcgtc gaacacggcg aggaccgtct tcaccgcctg
     2701 cggcttcttg aggatgcgca cgacgatctt ggtggcgacg ccgaaggtgc cctcggagcc
     2761 gacgaagatg cccggcagat cgtagccggc ggactcgcgc gtcttgccgc ccaaccagac
     2821 gatctcgccg tcgggcatga cgacctcgag cccgagcacg tggttgacgg tcaccccgta
     2881 cttcagggtg tgcggcccgc cggagttgtt ggcgatgttg ccgccgatgg tgcacgcctg
     2941 ctggctcgac gggtcgggcg catagtagta accggcgggg ccgaccgccc acgagagatg
     3001 caggttgacg aggcccggct cgacgaccgc gatctggttg tcgtagtcca cctcgagcac
     3061 gcggttcatc cgcatcagcg agatgacgat cccgccctcg gcgggcaggc agccgccgct
     3121 gagccccgt
//
LOCUS       DATIAZ010000070         6371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1068331, whole genome shotgun sequence.
ACCESSION   DATIAZ010000070 DATIAZ010000000
VERSION     DATIAZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068331"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1123
                     /locus_tag="VLF19_02275"
     CDS             143..1123
                     /locus_tag="VLF19_02275"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92106.1"
                     /translation="MDRRDFIVGGVAALAAPFSAEAQPAGKISRVGVLRPGNPPPGDF
                     GHREAFEGGLRDLGWTPGTDLLIEYRYADGKPERLSELATELVRLAVDVIVAGATTGA
                     RAAQQATRTIPIVMSTLPDPVGAGLVASLARPGGNMTGLTLDSEEMTGKQLELLKEVV
                     PRLSSVAVLGNPSSHAALPRQTAAAGAALGLKVQDFLVSHARDLAPTFAAMRQARVGA
                     ILVLRDPGVIERSLAEVVALAARQRLPALYFFREFPEAGGLMSYGANVKDIHRRSAAF
                     VDKILKGARPADLPIQQPTKFELVINLKTAKALGLTIPQSLLLRADHVLQ"
     gene            1413..1658
                     /locus_tag="VLF19_02280"
     CDS             1413..1658
                     /locus_tag="VLF19_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92107.1"
                     /translation="MDINSASETDLKALPGIGDAYAKKIDVWGATPTARARSKVDWGA
                     YLNNRFLIGTLFNVLCGVVGAAIGWFVLRPLKQGVAL"
     gene            complement(1765..1841)
                     /locus_tag="VLF19_02285"
     tRNA            complement(1765..1841)
                     /locus_tag="VLF19_02285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:Pro,seq:tgg)
     gene            complement(1877..2767)
                     /locus_tag="VLF19_02290"
     CDS             complement(1877..2767)
                     /locus_tag="VLF19_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92108.1"
                     /translation="MVGLLLLGACALREGPIGPGDAGLRLGFEERCARPGVVKCFGFD
                     SADATDRFVFPPHGLSVKRGRVVTDVKASGAGSLRFEIPSSSGADTSGSFWQNFADDR
                     SVQFGQGEEFYVQWRQRFSPELLRTRYQGGGGWKQAIIGEGDRPGKTAYSCTQLEIVV
                     QNGYHRGYPQMYHSCGGKDGQYQGLPEARWVSYRADQWMTFQVRVKIGTWYRNDRRYH
                     RDSAIELWVAEEGAASRLAVSHTRYDIANTDPEARYGKVWLLPYNTGKSPAHSHPIAY
                     TWYDELVISRFRIADPAGSR"
     gene            complement(2821..3441)
                     /gene="rdgB"
                     /locus_tag="VLF19_02295"
     CDS             complement(2821..3441)
                     /gene="rdgB"
                     /locus_tag="VLF19_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HSE92109.1"
                     /translation="MAPRRLLVLATLNTGKVREIAAMLAGLPFDVRSLAEVPGASLPP
                     ESADSYAANALVKARTAAALTGALALGDDSGLEVDALGGGPGVLTARYGGPGLDDAGR
                     YRRLLQALADVPEGRRRARFRCVIAIVEPGGTEHVVEGVAEGAIAREPRGQGGFGFDP
                     VFLYPPLGRTFGELSDADKAAVSHRGRAVRAARRVLADCYAPALGA"
     gene            complement(3441..4172)
                     /gene="rph"
                     /locus_tag="VLF19_02300"
     CDS             complement(3441..4172)
                     /gene="rph"
                     /locus_tag="VLF19_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSE92110.1"
                     /translation="MARPDGRAPDQLRPVVITRDFLVHPEGSVLVEFGATKVICTASV
                     EDKVPPFLKGQGVGWVTAEYGMLPRATNTRSSRENRGPSGRSQEIQRLVGRALRAVVD
                     RARFPERTVWVDCDVIQADGGTRTAAITGALVAVADAIGRMPGVDRAAALRDCVAAVS
                     VGVVEGRAVLDLNYAEDSSADVDMNVVMTGAGAFVEVQGTAERQAFGADRLNEMLALA
                     GAGIRRLVALQRRALESRDERTLRL"
     gene            complement(4176..5681)
                     /locus_tag="VLF19_02305"
     CDS             complement(4176..5681)
                     /locus_tag="VLF19_02305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSE92111.1"
                     /translation="MSVLVLGLVLLLAADARGGQLPTIVHEGRAYVSLDRLAATLDTR
                     PDATPSSIRAYLRVPGHVVTLTRNWAHVDVNGAALVLESPVRVRAGQWLVPAGFVKRV
                     LPRLAAPAPPASPRAVAAVAGPARVTAQTAQLSPSGPVPPARSGARASLEDLRYRSYP
                     SFTRLVLETSDAVRHTVEPAGGRELRVRIEGLAGEPRVEEIRDGLVDEVTLERAGGDA
                     VLRVRFEGVPGETKVTTLDDPHRLVLDLRRPVEAEERDRGAVTPLRLIVLDAGHGGHD
                     SGAVGPQGLMEKDLVLDVTKRVAKLVADRLGVKVRLTRDGDHFVTLRDRTRFANRERA
                     DLFVSIHANAHPQAANTGVETYFLSSEATDSGARQVAALENSVIQLEAPSPRGARQQD
                     VVKAILWDLAQSEFQAESSRLAEIVQDSMTQSLRIANRGVKQAGFYVLGGAAMPAVLV
                     EIGFVTNPGEERRLKDGRYRDDIARAIFTGLAEYKRNWDQRARTTAEQRPR"
     gene            complement(5735..>6371)
                     /locus_tag="VLF19_02310"
     CDS             complement(5735..>6371)
                     /locus_tag="VLF19_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HSE92112.1"
                     /translation="FGTIVRAIAEGRQLFRNLKMSFAYLVMIHVPIVVSAAVLPAAGY
                     PLLYLPAHIVWLELLIHPTALLVFQDLPAGGRLAPAGRGRRPRFFSAAEWAVLLGVGA
                     LLAALVVGAYLGAAGEAGDLAHGRSMAIAVVTTASAAITAALSRLRTRAAWLVIAGTL
                     ALSATLIQTRVLATALHVTPLHLGDWAAALAAGLLGGALAITLERAGTRGT"
BASE COUNT          966 a         2298 c         2228 g          879 t
ORIGIN      
        1 tccagggcgg ccacctgttg cttccgactg tgcaaatcgc agccgacgag gatcatgaga
       61 gcggtctcct ttctcgtggc gctgtcgcgc tcgggtggac tgagcgtaac aaccccgcgg
      121 aaccgttctc atgacatcaa tagtggaccg gcgggacttc atcgtcggtg gggtggccgc
      181 gctcgccgcg ccgttcagcg ctgaggcgca gccggcgggg aagatctctc gcgtcggcgt
      241 gctccggccg ggcaaccctc ctcccggcga ctttggccac cgggaggcgt tcgagggggg
      301 cctccgcgac ctgggctgga cgccggggac cgacctcctc atcgagtacc ggtatgccga
      361 tggcaagcct gagcgcctgt ccgaactggc aaccgagttg gttcggctcg ccgtggacgt
      421 gatcgtggct ggcgcgacta cgggcgcccg ggcggcacag caggcgactc gcacgatccc
      481 gatcgtgatg tccacactcc ccgatccggt tggagccgga ctcgtcgcga gcctggctcg
      541 ccctggcggc aacatgaccg ggctcaccct cgattccgag gagatgaccg gcaagcaact
      601 cgagctgctc aaggaagtcg tgccgaggct ctcaagcgtc gccgtcctcg ggaacccaag
      661 ctcgcacgcc gccttgccga ggcagaccgc ggcggctggg gccgcgctcg ggctcaaggt
      721 gcaagacttt ctggtgagcc atgccaggga cctcgcccca acgtttgcgg ccatgcgcca
      781 ggcccgggtg ggtgccatcc tcgttctacg cgatccaggg gtcatcgagc gcagtctggc
      841 cgaggtcgtg gccctggctg cacggcagcg gctgccagcc ctgtacttct tccgcgagtt
      901 ccccgaggct ggcggcctca tgtcctacgg tgcgaacgtt aaggacatcc accggcgctc
      961 ggcggcattc gtggacaaga tcctgaaggg ggcgaggcca gccgaccttc ccatccaaca
     1021 gccgacgaag ttcgagctcg tcatcaacct gaaaacggcg aaagcactgg gcctcaccat
     1081 tccgcaatcc ctgctgctcc gggcggatca cgtgcttcag tgaggaaccg tcgagcgcgc
     1141 caaaaccttg aagggtggtt gcgccggaag accgccccgc cagttcctaa acctgcacgt
     1201 ccgccaggcc gtccgcggcc gccgcgtccg gctccgccaa agcctcgtta gagtgcctcg
     1261 gacggtcgct catgctgagg atcatcgcag gactcatcgc agccgtgttc gcgctgggga
     1321 cgctcgccgc gcccgtttac gccgccggca aggaagcccc aaagacggag acgaagtagc
     1381 cggccaagcc ggctgcgccc aagaaggagc cgctggacat caacagtgct tcggagaccg
     1441 acctgaaggc cctgcccggc atcggcgatg cctacgccaa gaagatcgat gtttggggcg
     1501 ctacacccac tgcgcgagcg cgctccaagg tcgattgggg ggcctacctc aacaacaggt
     1561 ttctcatcgg cacgctcttc aacgtactgt gcggcgtagt tggtgctgcc attggctggt
     1621 tcgttctccg gccgttgaag cagggcgtcg cgctatgagc gccatctacg cccgcaagga
     1681 aggagggata tctcaggtag acttcggcta tcgacaacct ggacaacacc tgtacaccga
     1741 gggaccccga cgcgggctaa gtagtggtcg gggtggccgg atttgaaccg gcggccccct
     1801 gctcccaaag caggtgcgct accaggctgc gccacacccc gacgccgggc tccgagtgga
     1861 ggagtatagc agccggtcag cgcgagccgg caggatcggc gatccggaag cgggagatga
     1921 cgagctcgtc gtaccaggtg tacgcgatcg ggtgagagtg ggcgggactc ttcccggtgt
     1981 tgtagggaag cagccacacc ttgccgtagc gcgcctccgg atcggtattg gcgatgtcgt
     2041 accgcgtgtg gctcacggcc aggcgggacg ccgctccctc ctcggcgacc cacagctcga
     2101 tcgcactgtc acggtgatag cggcggtcgt tgcggtacca ggtgccgatc ttcacccgca
     2161 cctggaaggt catccactgg tcggcacgat acgacaccca cctggcctcg ggcagcccct
     2221 ggtactgacc atccttgccg ccgcagctgt gatacatctg cgggtagccc cgatggtagc
     2281 cgttctgcac cacgatctcg agctgggtgc aggaataggc ggtcttcccc ggccggtccc
     2341 cctcgccgat gatggcctgc ttccagcccc cgcccccctg gtatcgcgtg cgcagcagct
     2401 ccggcgagaa ccgttgccgc cactggacgt agaactcctc gccctgtccg aactgcacgc
     2461 tgcggtcgtc ggcgaagttc tgccagaagc tccccgaggt gtcggcgccg gagctggacg
     2521 ggatctcgaa gcgcagtgac cccgcgcccg acgccttgac gtcggtcacc acccgtccgc
     2581 gcttgaccga gaggccgtgg ggagggaaga cgaaccggtc ggtcgcatcc gcggaatcga
     2641 agccgaagca cttcacgacg ccgggacggg cgcaccgctc ctcgaatccg agccgcaggc
     2701 cggcgtcgcc cggaccgatc ggtccctcac ggagcgcgca cgcccccagc aggagaagcc
     2761 cgaccacgat gcaaggtcgg ctgagaggat tcgattttcc aggcggcgcc acgaccgggc
     2821 tcaggctccg agcgcaggag catagcagtc agccaggacg cgtcgcgccg cgcgcaccgc
     2881 gcggccgcgg tgactcaccg ccgccttgtc ggcgtccgag agctcgccga aggtccgccc
     2941 gagcggcgga tataagaaga ccgggtcgaa gccgaagccg ccctgcccgc gcggctcccg
     3001 ggcgatcgcg ccctcggcga cgccctccac cacgtgctcg gtgccgccgg gctccacgat
     3061 cgcgatgacg cagcggaagc gcgcgcgacg gcgaccctcg ggcacatcgg ccagcgcctg
     3121 gagcagccgg cgatagcgtc cggcgtcgtc gagcccgggg ccgccgtagc gcgcggtcag
     3181 gactcccggg ccgccgccga gtgcgtcgac ctcgagcccc gagtcgtcgc cgagggcgag
     3241 ggccccggtc agcgccgccg cggtccgggc cttgacgagg gcgttggcgg cgtaagagtc
     3301 ggccgattcc ggtggcagcg aggcgccggg cacctcggcg agcgaacgga cgtcgaacgg
     3361 cagtccggcg agcatcgcgg cgatctcccg caccttgccc gtgttgagcg tggcgagcac
     3421 gagcagccgc cgaggagcca ctacagcctc agggtgcgct cgtcgcgcga ctccagtgcc
     3481 cgacgctgca gggcgaccag ccggcggatg ccggcgccgg cgagggcgag catctcgttc
     3541 aggcggtcgg cgccgaaagc ctggcgttct gcggtgccct gcacctcgac gaacgcgccg
     3601 gcgccggtca tcacgacgtt catgtcgacg tcggccgacg agtcctcggc gtagttgagg
     3661 tcgaggacgg cgcgcccctc caccacgccg acgctcaccg cggccacgca gtcgcgcagc
     3721 gccgcggccc ggtccacgcc gggcatccgg ccgatggcgt cggcgacggc cacgagggcg
     3781 ccggtgatcg ccgccgtgcg ggtgccgccg tcggcctgga tcacgtcgca gtccacccag
     3841 accgtccgct ccgggaaccg ggcgcggtcg acgacggcgc gcagcgcgcg gcccacgagc
     3901 cgctggatct cctgcgaacg cccgctcggc ccccggttct cgcgggacga gcgcgtgttg
     3961 gtcgcgcgcg gcagcatgcc gtactccgcg gtcacccagc ccacgccctg gcccttgagg
     4021 aacggcggca ccttgtcctc gacggacgcc gtgcagatca ccttcgtcgc gccgaactcg
     4081 acgagcacgg agccttcggg gtgcacgagg aagtcacggg tgatcacgac gggccggagc
     4141 tgatcgggag cgcggccgtc gggacgagcc atggctcagc ggggccgctg ctcggcggtc
     4201 gtgcgcgcgc gctggtccca gttgcgcttg tactcggcca ggccggtgaa gatcgcgcgc
     4261 gcgatgtcgt cgcgatagcg cccgtccttc agccggcgct cctcgcccgg gttggtcacg
     4321 aacccgatct cgacgaggac ggccggcatg gcggcgccgc ccagcacgta gaagccggcc
     4381 tgcttgacgc cccggttggc gatgcgcagc gactgggtca tcgagtcttg cacgatctcg
     4441 gccagtcgcg acgactccgc ctggaactcc gactgcgcga ggtcccacag gatcgccttc
     4501 accacgtcct gctgccgggc gccgcgcggc gagggcgcct cgagctggat gacgctgttc
     4561 tcgagcgccg ccacctggcg cgcgccgctg tccgtcgcct ccgacgacag gaagtacgtc
     4621 tcgacacccg tgttcgccgc ctgggggtgc gcgttcgcgt ggatcgacac gaacaggtcc
     4681 gcgcgctccc ggttggcgaa gcgcgtccgg tcgcgcagcg tgacgaagtg gtcgccgtcg
     4741 cgggtgagcc gcaccttcac gccgagcctg tccgcgacga gcttcgccac gcgcttcgtc
     4801 acgtcgagga ccagatcctt ctccatcagc ccctgtggcc ccacggcgcc cgagtcgtgg
     4861 ccgccgtgtc cggcgtcgag cacgatgagc cggagcggcg tgacggcgcc gcggtcacgc
     4921 tcctcggcct cgacgggccg ccggaggtcg agcacgagcc gatgggggtc gtcgagcgtc
     4981 gtcaccttcg tctcgccggg cacgccctcg aagcgcacgc gcagcacggc gtccccgccg
     5041 gcgcgctcga gcgtcacctc gtccacgagc ccgtcgcgga tctcctccac gcgcggctcg
     5101 cccgcgaggc cctcgatgcg tacccgcagc tcgcggccac ccgccggctc caccgtgtgc
     5161 ctgacggcgt cggacgtctc gaggacgagc cgcgtgaagg acggatacga ccgatagcgc
     5221 agatcctcga gcgacgcgcg ggcgcccgac cgcgccgggg gaaccggccc cgacggcgag
     5281 agctgcgccg tctgcgcggt cacccgcgcc ggaccggcca cggcggcgac ggcccgcggc
     5341 gacgctggcg gcgccggcgc ggcgaggcgc gggagcaccc gcttcacgaa tcccgcgggc
     5401 acgagccact ggcccgcgcg cacgcgcacc ggcgactcca gcaccagcgc cgcaccgttg
     5461 acgtcgacgt gggcccagtt ccgggtgagc gtgacgacgt ggccgggaac cctcaggtac
     5521 gcgcggatgc tgctgggcgt ggcgtcgggc cgcgtgtcga gcgtcgcggc gagccggtcg
     5581 agcgacacat aggcgcggcc ctcgtggacg atcgtcggca gctggccgcc gcgcgcgtcg
     5641 gcggcgagca ggagcacgag cccgaggacg aggacggaca cccggcgcat cggtgcccac
     5701 attctagcaa cgcgcgcggg cagccccggg cggtttatgt cccccgggtg cccgcccgct
     5761 cgagcgtgat cgccagcgcc ccgccgagca ggccggccgc cagcgccgcg gcccagtccc
     5821 cgaggtgcag gggcgtgacg tgcagcgccg ttgccagtac gcgcgtctgg atcagcgtcg
     5881 ccgacagggc cagcgtcccc gcgatcacga gccaggccgc ccgcgtgcgg agccggctca
     5941 gcgcggcggt gatcgccgcg ctcgccgtcg tgaccacggc aatcgccatg ctccgtccat
     6001 gcgcgaggtc gccggcctcc ccggcggcgc ccaggtaggc gccgacgacg agcgcggcga
     6061 gcagggcgcc gacgccgagc agcacggccc actcggccgc cgagaagaag cgcggccgcc
     6121 ggccacgccc cgccggagcc aatcggccgc ccgccggcag atcctggaac acgagcagcg
     6181 ccgtgggatg gatgagcagc tccagccaca cgatgtgcgc tggcaggtag agcagcgggt
     6241 agcccgccgc cggcagcacg gccgccgaga cgacgatggg cacgtggatc atcacgaggt
     6301 acgcgaagct catcttgagg ttccggaaga gctggcggcc ttccgcgatc gcgcgcacga
     6361 tcgtgccgaa g
//
LOCUS       DATIAZ010000071         6204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_543103, whole genome shotgun sequence.
ACCESSION   DATIAZ010000071 DATIAZ010000000
VERSION     DATIAZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543103"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VLF19_02315"
     CDS             complement(<1..691)
                     /locus_tag="VLF19_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSE92113.1"
                     /translation="MTAPGVELVDRLRPLARDLLRDTVRRLAHLAYADLRLEVVESKA
                     ASAENGGPKFSGDDYRFTLGVRVLAGDRAVAPGYVGLVLGAADLPQIERVMREALERA
                     WRRATVNGEMKADARGKFGALGEALADLRLHPVEVREDVVPATYEIDPRSVALPVMVA
                     QATDISSRLSALDRRIGYNHVGVVTQLSRELFASTEGALIDQAFALTQGSVSVVAVGD
                     DNSQDLYDVMGH"
     gene            complement(688..1893)
                     /gene="glp"
                     /locus_tag="VLF19_02320"
     CDS             complement(688..1893)
                     /gene="glp"
                     /locus_tag="VLF19_02320"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSE92114.1"
                     /translation="MISVRDAQNHILAQVTERTPPELIALTDALSRVLADPIRAPFDV
                     PPTDNSAVDGYAVAGDDVPAGGARELVVVADLPAGSVFAGTLARGQAIRIMTGAPMPA
                     GADTVYPQEVVERTHDRVTVGPIARAANVRRRGEDVRSGTVVVEAGTVLRPQELGLIA
                     SLGSPTVVVTQRPRVALLSTGDEVAEPGGSRKPGQIYDANRFTLRGLSEQSGARVIDL
                     GIVPDQHDALREQLIAASEAADVVITSGGVSVGVYDLVKTVLAEIGGIDFWQVAMQPG
                     RPLAVGRIGSTHFFGLPGNPVASMLTFLLFVRPALLTLAGRRRVFPEPWPARATEAMP
                     KKRGRREFKRGVARFEDGAWTVRTTGPQGSGILSSMVAGNCLIVLEEERGDVAPGETV
                     LIEPLEPPA"
     gene            1913..2509
                     /locus_tag="VLF19_02325"
     CDS             1913..2509
                     /locus_tag="VLF19_02325"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSE92115.1"
                     /translation="MSDGGARPTRPAGFWIRLVAALVDGVVVSLAQWSMTFVAARVWG
                     REIDGSPGFQGLLGAFTLLFACVYSTVAHAATGQTIGKVLVGVRVSAVDGAPLAVGAA
                     LLRWFALFLSALPLGLGFVMAGLRTDKRALHDLIAGSRVQWVAPVRVEPHEPAPSPAL
                     GSDPVASPLTAAPWPRGGPGAAPSPEAPPPPAAPPTGA"
     gene            2431..3063
                     /locus_tag="VLF19_02330"
     CDS             2431..3063
                     /locus_tag="VLF19_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HSE92116.1"
                     /translation="MATRRSRRRAVSGGSTAARRAAHGRVIPTAPRRARNRLATAVLA
                     ALGDAPARRLLERVATDRDALGAWLAPDERGSIEQLWRRASAAAAAVRTLPLADPASS
                     LAAALAAAAALFDAGLGFEVHEALEPHWVTAEGERREALQGLIQVAVGFQHLANGNLD
                     GGRTLVGEGAARMRGRYLDGLALDDFARAAVGALVDLAAGEAPRPPRFPR"
     gene            3160..3954
                     /locus_tag="VLF19_02335"
     CDS             3160..3954
                     /locus_tag="VLF19_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE92117.1"
                     /translation="MDLELKGKTALITGASRGIGRAIALGLAREGARVALCARSAETL
                     ATSAADIRERLGADVVTIVTDLSSPEGVRRAVDGALRGLGSIDVLVNNAGAIRGGDFL
                     KIPDEQWMEDWSLKLLGYVRMCRAVLPLMQAQGGGRICNVVGAAARNPSPGYLVGGAA
                     NAALVNFTKGLADLGAKSNILVSAVSPAATRTERWDSLMAERAAASGRTLEQQRAEQE
                     GAYPLGRIATPEDIADLVCFLVSARASFITGICITVDGGSSRGVYP"
     gene            4085..4420
                     /locus_tag="VLF19_02340"
     CDS             4085..4420
                     /locus_tag="VLF19_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSE92118.1"
                     /translation="MAAKLFVGNLSFQATEEDLRELFAQAGTVETVRIITDQFTGRPR
                     GFGFVEMATKEEATKAIEMLNGRLFRDRNLVVDEARPQPQRGPGGGDRGPRGPRSGGG
                     AGGGGGWRR"
     gene            4549..5490
                     /locus_tag="VLF19_02345"
     CDS             4549..5490
                     /locus_tag="VLF19_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSE92119.1"
                     /translation="MAPRMGHVVDARREVEFREMQSAGLVNRVTGRSLPFGWTVNPYR
                     GCEMGCHYCFARYTHGFLGFTDPREFERTVYVKRIERTKLVAELRRARRSGLAVAMGT
                     ATDPYQPAEAQQRVARLVLEAVREVPGLRLSITTKSALVTRDLDLLSALAEYSEVSVN
                     ISITTLDAALARRLEPRAPRPDLRLATLAAVARAGVSARMFVMPVLPFITDGDESLRT
                     LLTAARAAGAAGAESNVLFLQPGTRETFFAFLEAERPELLTRYQGLYDGSAYARADYV
                     AEIEARVRRIAAEVGIAQRCRADRPIARPAEQLGLIF"
     gene            complement(5587..>6204)
                     /locus_tag="VLF19_02350"
     CDS             complement(5587..>6204)
                     /locus_tag="VLF19_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HSE92120.1"
                     /translation="LPEGIRLELDGRYAAMLSYKLKTYALLDGRGRVSLKGSGFRSRG
                     LEPFQRRLIEEVIHLLLVGRGLEVKAVVDRWLENFAARRVPPRLFARTETLGETLESY
                     RERVRAGDRNASAAYELAAASGRAWQPGDQVSYYVAGRGANVPVNEHAKLLGAWNPDH
                     RDENVEFYQARVLEVWERFRPFVDADGLRPPAEPGSPDSPQLSLF"
BASE COUNT          848 a         2198 c         2274 g          884 t
ORIGIN      
        1 ggtggcccat gacgtcgtag agatcctggc tgttgtcgtc gccgaccgcg acgacggaca
       61 cactgccctg ggtgagggcg aacgcctggt cgatcagcgc tccctccgtc gaggcgaaca
      121 gctcccgcga gagctgggtg acaaccccga cgtggttgta tccgatccgg cggtccagcg
      181 cggacaagcg gctggagatg tccgtggcct gggcgaccat cacgggcaga gcgaccgagc
      241 gcgggtcgat ctcgtacgtg gcgggcacga cgtcctcacg gacctcgacg ggatgcagtc
      301 ggagatcggc gagcgcctcg cccagcgcgc cgaactttcc ccgggcgtcg gccttcatct
      361 cgccgttgac ggtggcccga cgccaggcgc gctcgagcgc ctcgcgcatc acgcgctcga
      421 tctggggcag gtcggccgcg cccaggacga ggccgacgta gccgggcgcc accgcgcggt
      481 cgccggcgag cacgcggact ccgagcgtga accggtagtc gtcgccggag aacttcggcc
      541 cgccgttctc ggccgacgcc gccttggact cgaccacttc gaggcgcaga tcggcgtagg
      601 cgaggtgggc caggcggcgc accgtgtcgc gtagcagatc gcgcgccagc ggccggagcc
      661 ggtcgaccag ctccacgccc ggagccgtca cgcgggcggc tccagcggct cgatcagcac
      721 cgtctcgccg ggtgcgacgt cgccgcgctc ctcctcgagg acgatcaggc agttgcccgc
      781 gaccatcgac gacaggatac ccgagccttg gggccctgtc gtgcgcacgg tccaggcacc
      841 gtcctcgaag cgcgccaccc cacgcttgaa ctcgcgccgg ccccgtttct tgggcatcgc
      901 ctcggtcgcc cgcgccggcc acggctcggg gaacacgcgg cgccgaccgg cgagcgtgag
      961 cagcgcgggg cgcacgaaca gcaggaacgt gagcatcgag gcgacgggat tgccgggcag
     1021 gccgaagaag tgcgtcgacc cgatgcgccc gacggcgagc gggcggccgg gctgcatcgc
     1081 cacctgccag aaatcgatgc cgccgatctc ggcgagcacg gtcttgacga ggtcgtagac
     1141 gccgacggag acgccgccgg aggtgatgac cacgtcggcc gcttccgacg ccgcgatcag
     1201 ttgctcgcgc agtgcgtcgt gctggtccgg cacgatgccg agatcgatca cgcgcgcgcc
     1261 gctctgctcc gagaggccgc gcagcgtgaa gcggttggcg tcgtagatct gaccgggctt
     1321 gcgcgagccg cccggctcgg cgacctcgtc gccggtcgag agcagcgcca cgcgcgggcg
     1381 ctgggtgacg accaccgtcg gcgatcccag cgaggcgatc agccccagct cctggggccg
     1441 cagcacggtc ccggcctcga cgacgaccgt gccgcttcgc acgtcctcgc cgcgcctgcg
     1501 gacgttggcg gcgcgggcga tggggccgac cgtcacgcgg tcgtgcgtgc gctcgacgac
     1561 ctcctgtgga tagacggtgt ccgccccggc gggcatgggc gcgccggtca tgatgcggat
     1621 cgcctggccg cgcgcgagcg tgccggcgaa gacggagccg gccggcaggt cggcgacgac
     1681 cacgagctcg cgggccccgc cggcgggaac gtcgtcgccg gcgacggcat agccgtccac
     1741 cgcggagttg tcggtcggtg gcacgtcgaa gggcgcgcgg atcggatcgg cgagcactcg
     1801 cgacagcgcg tcggtgaggg cgatcagctc gggaggcgtt cgctcggtga cctgggcgag
     1861 gatgtgattt tgcgcatccc gcaccgaaat catgcccggt attatagcaa ccgtgagcga
     1921 cggcggtgcg cgtcccaccc gtcctgcagg cttctggatc cgcctcgtcg ccgcgctcgt
     1981 tgacggcgtc gtggtctcgc tcgcgcagtg gtcgatgacg ttcgtcgcgg cgcgcgtctg
     2041 gggccgcgag atcgacgggt cgccgggctt ccagggcctc ctcggcgcct tcacgctgct
     2101 cttcgcctgc gtgtacagca cggtggcgca cgcggcgacc ggccagacga tcggcaaggt
     2161 gctggtgggc gttcgcgtca gcgccgtgga cggcgcgccg ctcgccgtcg gcgccgcgct
     2221 gctgcgctgg ttcgcgctct tcctctccgc cctgccgctc gggctgggct tcgtcatggc
     2281 ggggctccgg accgacaagc gcgcgctcca cgacctcatc gccggtagcc gggtgcagtg
     2341 ggtggcgccg gtgcgggtcg agccgcacga gccagcgccc tccccggctc tcgggtcgga
     2401 cccggtcgca agcccgctca cggcggcgcc gtggccacgc ggcggtcccg gcgccgcgcc
     2461 gtctccggag gctccaccgc cgcccgccgc gccgcccacg ggcgcgtgat cccgacggcg
     2521 ccgcggcggg cgcgcaaccg gctcgccacc gccgtgctcg ccgcgctggg cgacgcgcca
     2581 gcgcggcggc ttctcgagcg ggtggcgacc gatcgtgacg cgctcggggc ctggctggcg
     2641 ccggacgagc gcggctcgat cgagcagctc tggcgacgcg ccagcgcggc ggcggcggcc
     2701 gtgcgcacgc tgccgctcgc cgatcccgct tcttcgctcg ccgctgcgct cgcggcggcg
     2761 gccgctctgt tcgacgcggg cctcggcttc gaggtacacg aggcgctcga gcctcactgg
     2821 gtgaccgccg agggcgagcg gcgtgaggcg ctgcaggggc tgatccaggt cgcggtcggc
     2881 ttccagcatc tcgccaacgg caacctcgac ggcggccgca cgctcgtcgg agaaggcgcc
     2941 gcgcgcatgc gtggccgcta cctcgacggc ctggccctcg acgacttcgc acgagcggcg
     3001 gtcggcgccc tggtcgatct cgccgcgggc gaggccccga ggccgccgcg ctttccgcgg
     3061 taagatacgg cgccgcgggg cgcgacccgt atcgaggcgc gccacggcgt tgccgggggt
     3121 gtgccgtgtc gtcgaacaaa cgccggaggt cacctgcaca tggatctcga gctgaagggc
     3181 aagacggcgc tgatcacggg ggcgagccgc gggatcggtc gcgccatcgc gcttggactg
     3241 gcgcgtgaag gagcccgagt cgcgctctgc gcacgcagcg cggaaaccct cgcgacctcg
     3301 gcggcggaca tccgcgagcg actcggcgcc gacgtggtga cgatcgtcac cgacctgtcc
     3361 tcgccggagg gcgtgaggcg cgcggtggac ggcgcgctca ggggactcgg gtcgatcgac
     3421 gtcctcgtca acaatgccgg ggccatccgc ggcggcgact tcctgaagat tccggacgag
     3481 cagtggatgg aggactggag cctcaagctc ctgggctacg ttcggatgtg ccgcgcggtc
     3541 ttaccgctca tgcaggcaca gggcggcggc cgcatctgca acgtcgtggg cgctgccgcg
     3601 cgcaacccgt cgcccgggta cctggtggga ggcgcggcga acgccgcgct ggtgaacttc
     3661 accaaggggc tggccgacct cggcgcgaag tccaacatcc tggtcagcgc ggtctctccg
     3721 gcggccacgc gcaccgagcg ctgggacagc ctcatggccg agcgggccgc cgcttcgggc
     3781 aggacgttgg agcaacagcg cgcggagcag gagggcgcct acccgctcgg acgcatcgca
     3841 acccccgagg acatcgccga cctcgtctgc ttcctggtct cggcgcgcgc gtcgttcatc
     3901 acgggcatct gcatcacggt ggacggcggc tcctcgcgcg gcgtgtatcc ctaaggccgg
     3961 ggcttttcac cagggtgacc ttgacaggcg ttacctcctg tcactactct tagcgcacgg
     4021 tctcggggag gtagtgcaaa cgtctcgagt acgccgcttg cactgagggc gggaggtcct
     4081 gcgcatggcc gcaaagcttt tcgtcggtaa cctgtccttc caggccacgg aggaggacct
     4141 ccgcgagctc ttcgcgcagg cgggtacggt ggagacggtc cgcatcatca cggaccagtt
     4201 cactggccgg ccgcgcggct tcggcttcgt ggagatggcc acgaaggaag aggcgaccaa
     4261 ggccatcgag atgctcaacg gacgtctctt cagggaccgc aacctcgtcg tcgacgaggc
     4321 caggccccag ccccagcgcg gtccgggtgg aggcgatcga gggccgcgcg gtccccggtc
     4381 cggcggcgga gcgggtggag gaggcggctg gcgccgctga tctcctggga gaccttccag
     4441 cccggggatg cgctctgcga ctccccgggc ttctttcggg ccaacgggac tcaacaggtg
     4501 atgagaacgt ggcgccccac aatcaagaag ggtataccgg cgcccggcat ggccccgcgc
     4561 atgggccatg tcgtggacgc gcggcgcgaa gtggagttcc gggaaatgca gtcggcaggg
     4621 ctcgtcaacc gtgtcaccgg gcgttcgcta cccttcggct ggactgtcaa tccgtaccgg
     4681 gggtgcgaga tgggatgcca ctactgcttc gcgcgctaca cccacgggtt cctcggcttc
     4741 accgacccgc gcgagttcga gcgcaccgtc tacgtgaagc ggatcgagcg gacgaagctc
     4801 gtggcggagc tgcgacgtgc acggcgcagc gggctcgccg tggcgatggg gacggcgact
     4861 gatccctatc aaccggccga ggcgcagcaa cgcgtcgcgc gcctcgtgct ggaggcggtg
     4921 agggaggtgc cgggcctccg gctctcgatc acgaccaaat ccgcgctggt cacgcgcgac
     4981 ctcgacctcc tctccgctct tgccgagtac tcggaggtga gcgtgaacat ttccatcacc
     5041 accctcgacg ccgcactggc gcggcgtctc gagccgcgcg cgccgcgtcc ggatctgcgg
     5101 ctggccacgc tcgccgccgt ggcccgggcg ggggtctctg cgaggatgtt cgtcatgccc
     5161 gtgctgccgt tcatcaccga cggcgacgag agcctgcgca cgctcctgac ggcggcccgt
     5221 gccgccggcg ccgccggcgc agagtccaac gtcctcttcc tgcagcctgg cacgcgcgag
     5281 accttcttcg ccttcctcga agcggagcgc ccggagctgc tcacccgcta tcaggggctc
     5341 tacgacggat cggcctacgc ccgtgccgac tacgtggccg agatcgaggc acgggtgcga
     5401 aggatcgcgg cggaggtcgg catcgcccag cgatgccgcg cggaccgtcc gatcgcgcgc
     5461 cccgccgagc aactcggcct gatcttctag gcctccgggc cgaacgcgcg gcgcgccgac
     5521 cggctcgcgg caggttcgag ggaccgcggg gtccgcgcgc gtcggcctcg gcgggctccc
     5581 gggctgctag aagagcgaca gctggggcga gtccggcgag ccgggctctg cgggtgggcg
     5641 caggccgtcg gcgtcgacga aggggcggaa gcgctcccac acctcgagca ccctggcctg
     5701 gtagaactcg acgttctcgt cccggtggtc gggattccac gcgccgagga gcttggcgtg
     5761 ctcgttgacc ggaacgttgg cgccgcgtcc ggcgacgtag taagagacct ggtcacccgg
     5821 ctgccaggcg cgccccgagg cggcggcgag ctcgtaggcc gcggaagcat tgcggtcgcc
     5881 ggcgcggact cgctcccgat agctctcgag ggtctcgccc agggtctcgg tgcgggcgaa
     5941 cagacgcggc ggcacccgtc gcgccgcgaa gttctccagc cagcgatcca ccacggcctt
     6001 gacctcgaga cctcgcccga cgagcagcag gtggatgact tcttcgatga gccggcgctg
     6061 gaacggctcg agaccgcggg agcggaatcc cgagcccttg aggctcacgc gcccgcggcc
     6121 gtcgagcagc gcatacgtct tgagcttgta gctcagcatc gccgcgtacc ggccgtcgag
     6181 ctcgagccgg atcccctcgg ggag
//
LOCUS       DATIAZ010000072         9695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_410961, whole genome shotgun sequence.
ACCESSION   DATIAZ010000072 DATIAZ010000000
VERSION     DATIAZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410961"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VLF19_02355"
     CDS             complement(<1..1220)
                     /locus_tag="VLF19_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSE92121.1"
                     /translation="MRTRPLLALALGLVLGLSLGALSTGLAGPPPTLLVQNGSPALTT
                     GTFAKLAEVVKPAVINVSSGSARDQRLPTEPGPGRRGLGSGFVIDPTGIALTNAHVVG
                     NSSQVDVTMLDGTKHRAKVVGVDRKTDLAVLKIEAAGKTFPHLTLADSDDARVGDWVV
                     AVGSPFGLQATVTSGIISAKARHIGAGPYDDFIQTDAAINPGNSGGPLVDMRGQVVGI
                     NTAVVHGGWGIGFAIPSNMAKRISTDLLASGTVTRGWLGVSLQPLTPALASSFGLTDT
                     RGALVAEVVPGSPAARAGLKSGDVITALDGETIAGPSDVARMVGLAKPGQERRLTVWR
                     DKREVKIQTRLDQAPGERARSRLGLDVRPVTPDVAEELRLRSPEGVVVADVEPESAAA
                     EAGIARGDVILEVDG"
     gene            1435..2583
                     /locus_tag="VLF19_02360"
     CDS             1435..2583
                     /locus_tag="VLF19_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92122.1"
                     /translation="MAGEEVYGSPEHQALRATVRKFVQTELAPRAREFDEAGRPDKAI
                     FRRLGELGLLGIRYDPRYGGQGLDYSYHAVFLEELTLCDNAGVAMGVSVQTDMATPAL
                     ARFGSEALKAKYLVPAIRGEQVAAIAVTEPSAGSDVAGIKTRAVRDGDHWVINGSKIF
                     ITNSANADWLCLLAVTDPGAGYKGYTQIIVPTDTPGFSYRLLDKIGNRGSDTGMLFFD
                     DVRVPVANTIGDPNRGFQQQMNQFQDERLVPVVTGPVAARRLWELTLAHAQQRVVFGK
                     PLARMQVNRFRFVEMMIQITAAQELAHRCIRKAVAGEDITLDVSMAKVFVANMKQYVA
                     TTCVQLFGGSGYIWENPAARAYVDWRLGTIGGGADEVMKEVAAKILKV"
     gene            2595..3176
                     /locus_tag="VLF19_02365"
     CDS             2595..3176
                     /locus_tag="VLF19_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSE92123.1"
                     /translation="MDALGLFMLRYDGIHVGFVEELFKNATDAQLRQRPHGANPVVWL
                     VWHATRVEDAVVNRLVADRPQVLEEGDWNRRMGVTRRDVGPGMTVEEVEALSARIDVP
                     ALRAYHAAVAAGTKAIASSLPPAGWDEIVPAERVREIVARDGLLIEAGRWVEEFWATG
                     RPRGWYLLQVGLLHPYGHWFDAMVTRGLLGVAP"
     gene            complement(3224..4243)
                     /locus_tag="VLF19_02370"
     CDS             complement(3224..4243)
                     /locus_tag="VLF19_02370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92124.1"
                     /translation="MDLGIFDHLDRREAPLHELYESRLRLLEKYDAAGFSAYHLAEHH
                     ATPLGLAPVPGIFLAAASQRTRRIRLGPCVYILPLYDPVRLVEEICMLDQLTRGRFDL
                     GVGRGIVPYEMAYFDLHHLETEEIYREALEVVLQGLTSDVLDHRGPRYTFRKVPMVLR
                     PFQRPHPPLWYGLGHLEGAEWAATNRVHVITNSTCEASKPLFERYREVWQRKHGTTPL
                     PKLGMSRHVVVAPTDARAEALARPSYAAWYANLTKLWRDFGSVPIRFARDFDEARQRG
                     IAITGTPARVREEIERQVAASGCTYLVCRLMFGTMSEAEAAASIDLFTAEVMPYLAAL
                     APVGV"
     gene            complement(4247..5374)
                     /locus_tag="VLF19_02375"
     CDS             complement(4247..5374)
                     /locus_tag="VLF19_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSE92125.1"
                     /translation="MTGRVAVLKAYGGDFELREYPVPDPEPGAILVRLTRAGVCGSDL
                     HIWRGEMKDVYGSLPRDLTFGHEMCGRVARLGAGVTVDSAGQPLREGDRVTFLYFFPC
                     GRCRVCQRDEMGACPRKGRPNRVAGTPPYFNNAYGDYYYLRPGGFVFRLPDEVSDDVA
                     TPANCALAQVIYGLTRAGVCLGDTVVIQGAGGLGLNAVAVARDLGADRVIVLDRVAAR
                     LELARAFGADQALSLADLSTPERRIQAVMDLTGGFGADVVADLVGYPEVVPEGLRMLR
                     SGGCYLEVGSIAPGNVFSYDTTALVRGNARLVATSNYSPWALAEALAFLRRHGTRLPF
                     DRLVSHVFPLSRIDEAFQQADWLPRGGDGLRISRAALSMDG"
     gene            5615..6079
                     /locus_tag="VLF19_02380"
     CDS             5615..6079
                     /locus_tag="VLF19_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92126.1"
                     /translation="MNIADAITDRVTGPIPPRVVNTTWPNVLHTRVEDVVRQMGPPPW
                     SRRIIADERQLVTLIANPPGGGNRPHWHADFDEWWVVLAGAIEWELTGGSVLRAGATD
                     VVWVPRGTVHHIRNAGADVSLRLAVAMPPAVHFYSPCEQCGFADDGPREWRA"
     gene            complement(6111..7340)
                     /locus_tag="VLF19_02385"
     CDS             complement(6111..7340)
                     /locus_tag="VLF19_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92127.1"
                     /translation="MERASVRHNFLALGLDYSCFLIGMSFASQATVLPAFAAHLGASN
                     VVIGAIPAVLTLGWFLPSLVAAHHTERLERKLPFVLRYTVWERVPMLALAAVAFFLAD
                     PSPRLALGVFFALILLMTGTGGTLSPAWMDVIGRAIPTTLRGRFFGATNVVAAAGGLL
                     GSVLVAWFLTAVPPPRSYALCFLAAFVFLAVSYVALASAREPAGGAAADPIPLTTYLR
                     RTPALLRRDANLRWFLVARAFGIFGTMANGFFTVYGLHALGAHEWHVGAFTTVFLAGQ
                     VVGNLALGWLADRAGHRLVLVLGLAAQAAGAVVAVGAASAEALAPVFALVGIHQASVH
                     VSARTILLEFAEDVAQRPTYIGLGNTALAPVSFAGPLAAGLVADHAGFTPIFGLAAAT
                     SAIAIALLLVRVREPRS"
     gene            7494..8639
                     /locus_tag="VLF19_02390"
     CDS             7494..8639
                     /locus_tag="VLF19_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HSE92128.1"
                     /translation="MTRTTGSIIAMAWVIGLAAAPAAAAPAGDAYIEGYAAAVLAERF
                     GRSPGTLHVKDGVITLAVSDLAGLDRDAVVAALRSIPGVERVDVTDTPDVKPAAPAAE
                     PTVAPPAGAGAPPTPPGGFELISRSEFEPGLMPGGQLFRPLVADPRWPHFSAAYQYYL
                     DDPDFGNVAAVSFGETFAIYRWDFTPHWLEVGVQAGVFAVFDLDADSSDLINADYFVA
                     AILGYRWNALSALARVFHQSSHLGDEFLLARSNVDRVNLSYEGVDVRISYELWDDVLR
                     PYAGAGYLFHRDPGDLAPWSVQYGLEFLWPGRDARVRPIAAADFQHREENDWGADLSL
                     RVGVQIGRTLGARAMQILLEYFSGHSPNGQFYQRKIEYLGLGVHFHF"
     gene            complement(8766..9491)
                     /locus_tag="VLF19_02395"
     CDS             complement(8766..9491)
                     /locus_tag="VLF19_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HSE92129.1"
                     /translation="MPLSASEFAKDLLREARQTHPWLHHPLFQMIYDGKLGKTQLRQV
                     VRQQGCFFLDTLRHAAWKIISVGGYTPTFDDLERQRALIPLVVEEGGEDTVGGKTTAH
                     AYLFVRLAEGLGITKEEIFATDYLPTTVIEKNELFLLQRDTLEALCGGNIATESINPI
                     HMRRMAEAMEEHYGVAREALDFYYVHMEVEGDHALRAVRILEKIAVTDEAQKRGRLAM
                     RRAITVRRVCADGMLQAFVTDQG"
     gene            complement(9502..>9695)
                     /locus_tag="VLF19_02400"
     CDS             complement(9502..>9695)
                     /locus_tag="VLF19_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE92130.1"
                     /translation="LSGKAMEARIEATVRDLESRVRPRLVAPGHCTGWRAKAALVRAF
                     APARYGPSAVGTSYVLTAG"
BASE COUNT         1476 a         3354 c         3529 g         1336 t
ORIGIN      
        1 ccgtcgactt cgaggatgac gtcgccgcgc gcgatgcccg cctcggcggc ggcgctttcc
       61 ggctccacgt cggcgaccac cacgccctcg ggcgatctca ggcgcaactc ctcggcgacg
      121 tcgggggtca ccggccggac gtcgagtccg agcctgctcc gcgcccgctc acccggcgcc
      181 tggtcgagcc tggtctggat cttcacctcc cgcttgtcgc gccacaccgt gagccggcgc
      241 tcctggccgg gcttggcgag gccgaccatg cgcgcgacgt cgctcgggcc ggcgatcgtc
      301 tcgccgtcga gcgcggtgat gacgtcaccg gacttgaggc cggcgcgggc ggcggggctg
      361 ccgggcacga cctcggcgac gagcgccccc ctcgtgtccg tcaggccgaa cgacgaggcg
      421 agggcgggcg tcagcggctg cagcgacact ccgagccagc cccgcgtgac ggtgccggag
      481 gcgagcaggt ccgtcgagat gcgcttggcc atgttcgacg gaatggcgaa tccgatgccc
      541 cagccgccgt ggacgacggc cgtgttgatg cccacgacct ggccgcgcat gtcgacgagc
      601 ggtccgcccg aattgccggg attgatggct gcgtcggtct ggatgaagtc gtcgtacggg
      661 cccgcgccga tgtgccgggc cttcgcgctg atgatgccgg acgtcaccgt ggcctgcagg
      721 ccgaacggcg agccgaccgc caccacccag tcgccgaccc gcgcgtcgtc cgagtccgcc
      781 agcgtcaggt ggggaaacgt cttgcctgcc gcctcgatct tgagcacggc gaggtcggtc
      841 ttccgatcga cgccgacgac cttggccctg tgcttcgtcc cgtcgagcat ggtgacgtcg
      901 acctggctcg agttgccgac gacgtgggcg ttggtgaggg cgatcccggt gggatcgatc
      961 acgaatccgg acccgaggcc ccgccggccg ggacccggct cggtcgggag ccgctggtcg
     1021 cgggcgcttc cgctgctcac gttgatgacg gccggcttca cgacctccgc gagcttcgcg
     1081 aaggtgccgg tggtcagcgc cggactgccg ttctggacga gcagggtcgg gggtggaccg
     1141 gcgagaccgg tggacaaagc gccgagggac agtcccagga ccaggccgag agcgagggcg
     1201 agcaacgggc gagtcctcat gggtaggggt tacctcctgg ataggttcga cggacatgga
     1261 acgggacgca ttcacgaggg gcggcggcca cgcgtccagc ctcctgacgc cgctccgctt
     1321 cgggggccgg caaaggagag gaagcgacag aaactctcca accgggtggc cgcgccgcac
     1381 agcatactcc aaagtgcgtg ccatcggccc ccggctctgc tagcgtttgt ggccatggcc
     1441 ggcgaggaag tctacggcag ccccgagcac caggcgctcc gcgccaccgt gcgcaagttc
     1501 gtccagaccg agctggcgcc ccgggcgcgg gagttcgacg aggcgggccg gcccgacaag
     1561 gcgatcttcc gccgcctggg cgagctgggg ctcctcggca tccgctacga cccgagatac
     1621 ggcggccagg ggctcgacta ctcgtaccac gccgtgttcc tcgaggagct gaccctctgc
     1681 gacaacgccg gggtggccat gggcgtgagc gtgcagaccg acatggcgac accggcgctc
     1741 gcccgcttcg gcagcgaggc gctcaaggcg aagtacctgg tgccggcgat ccgcggcgag
     1801 caggtcgccg ccatcgccgt gaccgagccg agcgccgggt ccgacgtggc cggcatcaag
     1861 acccgcgcgg tgcgcgacgg cgaccactgg gtgatcaacg gctccaagat cttcatcacg
     1921 aactcggcca acgccgactg gctgtgtctg ctggcggtga ccgacccggg cgcgggctac
     1981 aagggctaca cgcagatcat cgtgcccacc gacaccccgg ggttctccta tcgcctgctc
     2041 gacaagatcg gcaaccgcgg ctccgacacc gggatgctgt tcttcgacga cgttcgcgtg
     2101 cccgtcgcca acaccatcgg cgaccccaat cgtggcttcc agcagcagat gaaccagttc
     2161 caggacgagc gcctggtgcc ggtggtgaca gggcccgtcg ccgcgcggcg gctgtgggag
     2221 ctgacgctcg cgcacgcgca gcagcgcgtc gtcttcggca agccgctggc caggatgcag
     2281 gtgaaccgct tcaggttcgt cgagatgatg atccagatca ccgccgccca ggagctggcg
     2341 caccgctgca tccgcaaggc ggtggccggc gaggacatca ccctcgacgt ctcgatggcc
     2401 aaggtcttcg tcgccaacat gaagcagtac gtggcgacga cctgtgtgca gctcttcggc
     2461 ggcagcggtt acatctggga gaaccccgcc gcccgcgcct acgtggactg gcgcctgggc
     2521 accatcggcg gcggcgccga cgaggtgatg aaggaagtcg ccgcgaagat cctgaaggtc
     2581 tgaggctccg aggaatggac gcgctcggcc tgttcatgct ccggtacgac ggcatccacg
     2641 tcggattcgt ggaggagctc ttcaagaacg ccaccgacgc ccagctgcgg cagcggccgc
     2701 acggagccaa cccggtggtc tggctcgtct ggcacgccac ccgggtcgag gacgcggtgg
     2761 tgaatcggct cgtggccgat cgtccgcagg tgctcgagga gggcgactgg aaccggcgca
     2821 tgggggtcac gcggcgcgac gtggggccgg ggatgaccgt cgaggaggtc gaggcattga
     2881 gtgcccggat cgacgtgccg gccctgcgcg cctatcacgc cgccgtcgcc gcgggaacga
     2941 aggcgatcgc gagctcgctg ccgccggcgg ggtgggacga gatcgtgccg gccgagcggg
     3001 tgcgtgagat cgtcgcgcgc gacgggctcc tgatcgaggc cgggcgctgg gtcgaggagt
     3061 tctgggccac ggggcggccc cgcggatggt acctgctgca ggtcggactg ctccacccgt
     3121 acgggcactg gttcgacgcg atggtgacgc gaggcttgct cggcgtcgcc ccctgagtca
     3181 gccgcccgct tgcacgagcg cggcgaggtg gggcacgagt cgttcacacg ccaacgggcg
     3241 cgagtgcggc gagatagggc atgacctcgg ccgtgaacag gtcgatcgac gccgcggcct
     3301 ccgcctcgct catcgtcccg aacatcagcc ggcacacgag ataggtgcat ccgctggcgg
     3361 cgacctgacg ctcgatctcc tcgcgcacgc gcgccggcgt tcccgtgatg gcgataccgc
     3421 gctggcgcgc ctcgtcgaag tcgcgggcga agcggatcgg gacggagccg aagtcgcgcc
     3481 acagcttggt gaggttcgcg taccacgcgg cgtagctcgg cctcgccagc gcctcggccc
     3541 gcgcgtcggt gggggccacg accacgtggc gcgacatgcc gagcttcggg agcggcgtgg
     3601 tcccgtgctt gcgctgccac acctcgcgat agcgctcgaa gagcggcttc gatgcctcac
     3661 acgtgctgtt cgtgatgacg tgtacccggt tggtcgccgc ccactcggct ccctcgaggt
     3721 gaccgagacc gtaccagagc ggcgggtgcg gccgctggaa gggccggagc accatcggca
     3781 ccttgcggaa ggtgtagcga ggcccgcggt ggtccagcac gtcgctggtg agtccctgca
     3841 ggacgacctc cagcgcctcg cggtagatct cctcggtctc gaggtggtgc aggtcgaagt
     3901 aggccatctc gtagggaacg atcccccggc ccacaccgag gtcgaagcgc ccccgcgtga
     3961 gctggtcgag catgcagatc tcctcgacga gccgcaccgg gtcgtagagc ggcaggatgt
     4021 acacgcacgg ccccaggcgg atccgccggg tccgctgcga ggcggcggcg aggaagatgc
     4081 ccggcaccgg tgccagtccc agcggcgtgg cgtggtgctc ggccaggtga taggcggaga
     4141 accccgcggc gtcgtacttc tcgagcaggc gcagccggct ctcgtagagc tcgtgcagcg
     4201 gtgcctcgcg ccggtcgaga tgatcgaaga tccccaggtc catcgctcag ccgtccatcg
     4261 acagggcggc ccgggagatg cgcaggccgt cgccgccgcg cggcaaccag tcggcctgct
     4321 ggaacgcctc gtcgatgcgc gacagcggaa agacgtggga gaccaggcgg tcgaagggca
     4381 gccgcgtgcc gtggcgccgc aggaaggcga gcgcctcggc gagcgcccac ggcgagtagt
     4441 tggacgtcgc cacgaggcgc gcgttgccgc ggacgagcgc cgtcgtgtcg taggagaaga
     4501 cgttgcccgg cgcgatggag ccgacctcca aatagcagcc gccgctgcgc agcatgcgga
     4561 ggccttccgg cacgacctcc ggatagccga cgaggtccgc gacgacgtcg gcgccgaagc
     4621 ctccggtgag atccatgacg gcctggatgc gacgctcggg ggtcgagaga tcggccaggc
     4681 tcagcgcctg atcggcgccg aacgcgcgcg ccagctcgag acgggcggcg acgcggtcga
     4741 gcacgatgac gcggtcggcg cccaggtcgc gggcgacggc caccgcgttg aggccgaggc
     4801 cgccggcgcc ctggatgacc accgtgtcgc cgagacacac cccggcgcgc gtgagcccgt
     4861 agatgacctg ggccagcgcg cagttcgccg gcgtcgcgac gtcgtcggag acctcgtccg
     4921 ggaggcggaa cacgaagccg ccgggccgga ggtagtagta gtcgccgtaa gcattgttga
     4981 agtagggcgg ggtgcccgcc acgcggttcg gccggccctt gcgcggacag gcgcccatct
     5041 cgtcgcgctg acagacgcgg cagcgaccgc acgggaagaa gtagaggaac gtgacccggt
     5101 cgccctcgcg gagtggctga cccgcggagt ccaccgtgac gccggcgccc aggcgcgcga
     5161 cgcggccgca catctcgtgg ccgaaggtga gatcgcgggg cagcgagccg tagacgtcct
     5221 tcatctcgcc ccgccagatg tgcaggtcgg aaccgcacac gccggcgcgc gtcagccgga
     5281 ccaggatggc gccgggctcc gggtccggca ccgggtactc ccgcagctcg aagtcgccgc
     5341 cgtacgcctt cagaacggcc acccgtccgg tcatggtcat cctccctggc cgcggccccg
     5401 cgtcgccggg cgtccagcac agtactcgac tgcgtgtcga agtgcacgtc ggccctggca
     5461 acccatgcgg cgtgtcggcc caggaccgtc gggtaacacc caaccccgag gtatcagcgt
     5521 cttgtcccgc gtcttgaccg caccccggct gtcgagcacc atcggaacgg acggctgaca
     5581 tcacacctgg cttccgtcca agggagacga gacggtgaac atcgccgacg cgatcacgga
     5641 ccgcgtgacg ggaccgatcc cgccacgcgt cgtcaacacc acgtggccga acgtgctgca
     5701 cacgcgagtg gaggacgtcg tccggcagat gggaccgccg ccctggtcgc gacggatcat
     5761 cgccgacgaa cgccagctcg tcacgctgat cgccaacccg cccggcggcg ggaaccggcc
     5821 gcactggcac gcggacttcg acgagtggtg ggtcgtcctc gccggggcga tcgagtggga
     5881 gctgacgggc ggctccgtgc tcagagccgg cgccacggac gtcgtctggg tgccgcgggg
     5941 gaccgtgcac cacatccgga acgccggcgc ggacgtgtcg ttgcgcctcg ccgtcgccat
     6001 gccgccggcc gtgcacttct acagcccctg cgagcagtgc ggcttcgccg acgacggacc
     6061 gcgcgagtgg cgggcctaga gacctcggag gggctcaccc gcctccgagg tcaggagcgg
     6121 ggctcgcgga cgcggacgag caggagcgcg atggcgatgg ccgacgtggc cgccgccaga
     6181 ccgaagatcg gggtgaagcc agcgtggtcg gcaacgagtc cggcggcgag cggcccggcg
     6241 aagctcacgg gcgccagggc cgtgttgccg agtccgatgt aggtcggccg ctgcgccacg
     6301 tcctccgcga actccagcag gatcgtccgc gcggagacgt ggaccgacgc ctggtggatc
     6361 ccgaccagcg cgaacaccgg cgccagcgcc tcggcggacg cggcgccaac cgcgacgaca
     6421 gcgcccgcgg cctgcgccgc gaggccgagc acgagcacga gtcggtgccc ggcgcggtcg
     6481 gcgagccacc cgagcgccag gttgccgacc acctgcccgg ccaggaagac cgtcgtgaac
     6541 gcgcccacgt gccactcgtg cgcgcccagc gcgtggagcc cgtagacggt gaagaagccg
     6601 ttggccatcg tcccgaagat cccgaaggcg cgggcgacga ggaaccagcg caggttggcg
     6661 tcgcgccgga gcagcgccgg cgtccggcgc agataggtcg tgagagggat gggatccgcg
     6721 gccgcgccgc cggcgggctc gcgcgcgctc gccagcgcga cgtaggaaac ggcgaggaac
     6781 acgaacgccg ccaggaagca gagcgcgtag ctccgcggcg gcggcaccgc ggtcaggaac
     6841 caggcgacga gcacgctgcc gagcagcccg ccggcggcgg ccaccacgtt ggtggcgccg
     6901 aagaaccggc cgcggagcgt ggtcggaatg gcgcgcccga tgacgtccat ccaggcgggc
     6961 gacagcgtgc cgccggtgcc cgtcatgagg aggatgagcg cgaagaagac gcccagcgcc
     7021 aggcgcggcg acggatcggc gaggaagaac gcgacggcgg ccagcgccaa catcggtacg
     7081 cgctcccaca ccgtgtatcg cagcacgaag gggagcttcc gctcgagccg ctccgtgtgg
     7141 tgggcggcga cgagtgacgg caggaaccac ccgagcgtca gcacggccgg gatggcgccg
     7201 atgacgacgt tcgaggcgcc gaggtgcgcg gcgaaggccg gcaggaccgt cgcctgggac
     7261 gcgaacgaca tgccgatcag gaagcaagaa tagtccagac ccagggccag gaagttgtgg
     7321 cgaacggatg cccgctccac gccgcaagct catcggaggg tggccgcgac cgcccctccg
     7381 aaacctcccc cgacatcagt gtcgccggcg gagccggcgc tcggccacgg ttactcggag
     7441 acaatcccag tgtagggtac gctgggtgtc gtcgcttccg cgaaggagga gccgtgacca
     7501 ggacgacagg gtcgatcatc gcgatggcgt gggtgatcgg gctggcggcg gcgcccgccg
     7561 cggcggcgcc cgccggcgac gcctacatcg aaggctatgc ggcggcggtg ctcgcggagc
     7621 ggttcggccg gtcgcccggc acgctccacg tgaaggacgg cgtgatcacg ctggcggtgt
     7681 cggatctcgc cggcctggat cgcgacgcgg tcgtcgctgc cctccgcagc attcctggcg
     7741 tcgagcgcgt ggacgtcacg gacacgcccg acgtgaagcc agcggcgccg gcggccgagc
     7801 ccaccgtcgc gccgccggcc ggggccggcg ccccgccgac cccgcccggc gggttcgagc
     7861 tgatatcgcg ctccgagttc gagccgggct tgatgccggg cggccagctc ttccggccgc
     7921 tggtcgccga cccccgctgg ccgcacttct ccgccgcgta ccagtactat ctggacgatc
     7981 ccgacttcgg caacgtcgct gccgtgagct tcggcgagac gttcgccatc taccggtggg
     8041 acttcacgcc gcactggctg gaggtcggtg tccaggccgg ggtcttcgcc gtcttcgacc
     8101 tcgacgcgga ctcgtccgac ctcatcaacg ccgactactt cgtggccgcc atcctcggct
     8161 accgctggaa cgcgctgtcg gcgctcgcgc gggtgttcca ccagagcagc catctgggcg
     8221 acgagttcct gctggcgcgc agcaacgtcg accgcgtcaa cctcagctac gagggagtcg
     8281 acgtccggat ttcctacgag ctgtgggacg acgtgctccg cccgtacgcc ggtgccgggt
     8341 atctgttcca ccgggatccc ggcgacctcg cgccgtggtc ggtgcaatac ggtctcgagt
     8401 ttctgtggcc gggccgggac gcgcgggtcc gcccgatcgc ggccgcggac ttccaacacc
     8461 gggaggagaa cgactggggc gccgacctct cgctccgggt gggcgtgcag atcggccgca
     8521 cgctcggggc gcgcgccatg cagatcctgc tcgagtactt cagcggccac tcgcccaacg
     8581 gccagtttta ccagcgcaag atcgaatacc tggggctggg cgtgcacttc cacttctgat
     8641 catggccggg gggcctgacg gttgatcatg gccggggggc ctgacggtcc cgcggaacag
     8701 attgcgcgac gacgcccgcg ctcgaccgcc gcgaatcccg aggcgccggc ggctgcggct
     8761 cggcgtcacc cctggtccgt cacgaacgcc tggagcatgc cgtcggcgca gacgcggcgg
     8821 acggtgatcg cgcggcgcat ggcgaggcgt ccacgcttct gggcctcgtc ggtcaccgcg
     8881 atcttctcga ggatgcgcac cgcgcgcaac gcgtggtctc cctcgacctc catgtggaca
     8941 tagtagaagt cgagcgcctc gcgtgcgacg ccgtagtgct cttccatggc ctcggccatc
     9001 cggcgcatgt ggatggggtt gatcgactcg gtggcgatgt tgccgccgca cagcgcctcc
     9061 agcgtgtcgc gctggagcag gaacagctcg ttcttctcga tgaccgtggt gggcaggtag
     9121 tcggtcgcga agatctcctc cttcgtgatg ccgagcccct cggccagccg gacgaacagg
     9181 tacgcgtgtg ccgtcgtctt gccgcccacg gtgtcctcgc cgccctcctc gacgacgagc
     9241 gggatgagcg cgcgctggcg ctcgaggtcg tcgaaggtcg gcgtgtagcc gcccacggag
     9301 atgatcttcc aggcggcgtg gcggagcgtg tccaggaaga agcaaccctg ctggcggacg
     9361 acctgacgga gctgcgtctt gccgagcttg ccgtcgtaga tcatctggaa gagcgggtgg
     9421 tgtagccagg ggtgcgtctg ccgggcctcg cgcagcagat cctttgcgaa ctcggacgcg
     9481 gagagcggca tggtcttcct cctagccggc cgtcaggacg tacgacgtgc cgacggcgct
     9541 cggcccgtac cgtgcggggg cgaacgcccg gacgagggcg gccttggccc gccaccccgt
     9601 acagtgcccg ggcgcgacga gccgtggccg gacccgcgac tcgagatcgc gcacggtcgc
     9661 ctcgatgcgc gcctccatcg ccttgccgct caggt
//
LOCUS       DATIAZ010000073         6501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1727667, whole genome shotgun sequence.
ACCESSION   DATIAZ010000073 DATIAZ010000000
VERSION     DATIAZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727667"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VLF19_02405"
     CDS             complement(<1..318)
                     /locus_tag="VLF19_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92131.1"
                     /translation="MNDEMRTASTVTRRSFLATSALTLAGTLGPWGAAPARAQKVEKL
                     TFGTNWKAQAEHGGYYQAVATGIYKKAGLDVTIRMGGPQINHPQLLAAGAIDFNMGSN
                     CFSA"
     gene            complement(556..1410)
                     /locus_tag="VLF19_02410"
     CDS             complement(556..1410)
                     /locus_tag="VLF19_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSE92132.1"
                     /translation="MLKKLPDKLGTGLHGAIPPARYWAYYTWQEIDAIARADRNATAV
                     INVGAVEQHGPHLPLITDTLVGMEMLGAALARLPDDVMVLALPPTNFGKSTEHITFPG
                     TLTFSGETLRMVLKDLAASVARSGFAKLVLLGSHGGNVGTLDDFFRDLRMETDLRVFK
                     IFLGGIGQVPGLVAPEEAAIAMHSGDSETSMVRHLAPELVHMDRAEGYVFDPTPDIGY
                     SFKGHDVIEAWVASDLSKTGAIGNPHRSSAEKGRQMCAAKAARLAELLEAIYRSPRRE
                     IRLAARDG"
     gene            complement(1428..2651)
                     /locus_tag="VLF19_02415"
     CDS             complement(1428..2651)
                     /locus_tag="VLF19_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92133.1"
                     /translation="MLTTRAVSTSLANARALEVEMHFGAFFYGTVDMPDAGVDGPPAH
                     RRNYGQEDYRRAYADLIAYTQACDTLGYDSVWSAEHHFHNHGFEVVPNVILLNAVLAQ
                     HTRRVRLGALIHVLTTWHPIHFAEDYALADVLSGGRLLCGLGRGTEERESNVFGVNVG
                     YDNNADDLHNREVFEEQVEIFKAATSNERFAYRGKYYTIPPEGLTFRGEPVTEFPLVP
                     RPVNTPVRVHQPISSEETLLYAARQRHVGVFANHPWDRMVPWWRRYGAAVHEAHGVTL
                     RPGEDRMLQVQIHIADSAEAAIRTARRGHDELTKLLWPNIVRRNPALTGRPPFTLEER
                     MTSRSWIVGTPEQVRDILVEMHDVLGYEALVIFPHLPGMRRPETLEQLGRFWADVRPA
                     LAARAPASQPVTAGV"
     gene            complement(2850..3644)
                     /locus_tag="VLF19_02420"
     CDS             complement(2850..3644)
                     /locus_tag="VLF19_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE92134.1"
                     /translation="MSERTLAVWQGKVRIRVLSEGDGPALVFFHGPWGLTWDPFLDEL
                     ARSFTVYAPEHPGTTPGAPDDVYHLDGLWDLVLCYDELMAALGLERAALVGHSFGGMV
                     ACEFAASYPGRARRLVLIDPLGFWRDSDPVVNWMTLEPSRMRAHIFRDPDGEAARRLF
                     GDAHDPETAAAARVRLMWAMGTTGKFIWPIPDKGLKKRIHRVTAPTLLVWGKEDRLVP
                     PVYADEFARRLPAARAETVEGAGHAPHLEQPETVARTIRDFLGSKT"
     gene            complement(3641..4894)
                     /locus_tag="VLF19_02425"
     CDS             complement(3641..4894)
                     /locus_tag="VLF19_02425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92135.1"
                     /translation="MKISAFHLMPHRELPDDFEQRYESVWVTPPWWELADAGRVGQYY
                     NWTLDELLHAARAGFDGLCTNEHHQNAYGFMPSPNLMGGILAKLTNGTNVAIVQMGAT
                     LPTSNPPIRVAEEYAMLDCISGGRLVAGLPLGSPMDVNLCYGITPMEHRERYREAFVL
                     TLKAWQAREIFAWNGRYYQLANVNLWPRPIQQPHPPVWVPGSGSISTFEFAVEHDVCY
                     CFLSYSGARSARTMMQGYWNVVAAKGRDPNPYRAGFLQLVAVAESDARAEEKYARHVE
                     YFYHKCLHVPGPWFSPPGNQDYRSLVASTKNPIRYAVNPKELRYRDFVDKGYVIAGSP
                     ATVRDRLEEEVIKGLRVGNLMVLLQIGSMPHELALENIDRFAGEVLPHLRTTWDDEGW
                     VNHWWPERLRAAPAPAVAGVAAGGA"
     gene            complement(5112..6308)
                     /locus_tag="VLF19_02430"
     CDS             complement(5112..6308)
                     /locus_tag="VLF19_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92136.1"
                     /translation="MVATVWVTLAIASGLYFSFPVFFVALLEEFGWSRGATAAAFSIS
                     SIVQGVLSPGVGMLVDRLGPRRVMLAGACLLGTACVLSSRIGSLWSLYALTGVLAAAG
                     VCAVSWIPSGALIARWFIERRGSMLGLAFSGMGAGVLAVGPLAQWLIAGYGWRTAYVV
                     LGAGTLLTLLPVIWLGVREAPVVAVTGPGRRGVEEPAARPGAGREVGDALRTRAFWAL
                     FFAYLCTPLAVFSVVTHQVAFAVDHGFPRLFVAGIFGLTGLLSVVGRVVFGIAADRIG
                     RTASATVSYGCTALGTLCLLGLEVWPHAAALYAYAIFFGLGFGARGPIITAIASQLFP
                     GRRFGVIYGILSVGSGIGGGIAPWYAGAVHDVTGSYRVAFLIAVGFCVLGSACFWLAR
                     PRRGSL"
BASE COUNT          990 a         2309 c         2291 g          911 t
ORIGIN      
        1 ggcgctgaag cagttgctgc ccatgttgaa gtcgatcgcg cccgcggcga ggagctgcgg
       61 atggttgatc tgcggcccgc ccatccggat cgtcacgtcg agaccggcct tcttgtagat
      121 gccggtggcc accgcctggt agtagccgcc gtgctcggcc tgcgccttcc agttggtgcc
      181 gaacgtcagc ttctcgacct tctgggcccg cgccggcgcg gccccccagg gaccgagcgt
      241 gccggccagc gtgagcgcgg acgtcgcgag gaacgaccgg cgcgtcaccg tcgaagccgt
      301 gcgcatctca tcgttcatgt tcgacctccc gaggttctgg atcagacgtt cgccgaccgt
      361 aggccgggac gggctcgggg tggggggcgc gggtccgccg acctgtcccc ctcccaccgg
      421 atcctcgccc cacccgacga tggaaacagc acccgaagga cgacgcgcaa cgcatcaggg
      481 gtcgccggat ccgcgccccc caccccgagc ccgtcccggc cggcgccttc gcccgcagac
      541 cgctcgtccg aaatactagc cgtccctggc cgcgagacgg atctcccgcc gcgggctccg
      601 gtagatcgcc tcgaggagct cggccagccg cgcggccttg gcggcgcaca tctgacgacc
      661 cttctcggcc gacgaccggt gggggtttcc gatggcgccg gtcttcgaga gatcggaggc
      721 cacccacgcc tcgatcacgt cgtgcccctt gaaggagtag ccgatgtccg gcgtgggatc
      781 gaagacgtag ccctcggccc ggtccatgtg caccagctcg ggggccaggt ggcggaccat
      841 cgaggtctcc gagtccccgg agtgcatggc gatggccgcc tcctcgggcg cgactaggcc
      901 gggcacctgg ccgatgccgc cgagaaagat cttgaagacg cgcaagtccg tctccatgcg
      961 caggtcgcgg aagaagtcgt cgagtgtccc gacgttgccg ccgtggcttc ccagcagcac
     1021 gagcttggcg aatccgctcc gggccaccga cgccgccagg tccttgagaa ccatgcgcag
     1081 ggtctcgccg ctgaaggtca gggtccccgg aaaggtgatg tgctccgtgc tcttgccgaa
     1141 gttggtcggc ggcagcgcca gcaccatcac gtcgtccggc aggcgggcca gtgccgcgcc
     1201 gagcatctcc attccgacga gcgtgtccgt gatcagcggc aggtgcgggc cgtgctgctc
     1261 gacggcgccg acgttgatca ccgcggtggc gttgcggtcg gccctggcga tggcgtcgat
     1321 ctcctgccag gtgtagtacg cccagtaccg ggcgggtggg atcgccccgt ggaggcccgt
     1381 cccgagcttg tcgggcagct tcttcagcat gctcgtcctc cggagcgtca cacgccggcc
     1441 gtgaccggct ggctcgccgg tgcgcgtgcg gcgagcgcgg gccgcacgtc ggcccagaag
     1501 cgcccgagct gctcgagggt ttccggccgc cgcatgcccg gcaggtgggg aaagatcacg
     1561 agcgcctcgt agccgagcac gtcgtgcatc tcgaccagga tgtcgcgcac ctgctccggg
     1621 gtgccgacga tccaggacct gctcgtcatg cgctcctcga gcgtgaacgg cgggcggccc
     1681 gtgagcgccg ggttgcggcg gacgatgttc ggccagagca gcttggtcag ctcgtcgtgc
     1741 ccacgccgcg cggtccggat cgccgcctcc gccgagtcgg cgatgtgtat ctggacctgg
     1801 agcatgcggt cctcgcccgg ccggagggtg acgccgtgcg cctcgtggac cgccgcgccg
     1861 taccgccgcc accaggggac catccgatcc cagggatggt tggcgaagac gcccacgtgg
     1921 cgctggcggg cggcgtagag gagcgtctcc tcgctgctga tgggctggtg gacgcgcacc
     1981 ggcgtgttga cgggccgcgg gaccagcggg aactccgtga ccggctcgcc gcggaacgtc
     2041 aggccctcgg gcgggatcgt gtagtacttg ccgcgatagg cgaaccgttc gttcgacgtg
     2101 gcggccttga agatctccac ctgctcctcg aagacctcgc ggttgtgaag atcgtcggcg
     2161 ttgttatcgt agccgacgtt gacgccgaac acgttcgatt cgcgctcctc ggtgccccgg
     2221 cccaggccac agagcagtcg cccgccggag aggacgtcgg ccagggcgta gtcctcggcg
     2281 aagtggatcg ggtgccaggt ggtcagcacg tggatcagcg cgccgaggcg gacccgacgc
     2341 gtgtgctggg ccagcacggc gttcaacaag atcacgttcg gcacgacctc gaacccgtga
     2401 ttgtggaagt ggtgctcggc cgaccacacc gagtcgtacc cgagcgtgtc gcaggcttgc
     2461 gtgtaggcga tgaggtcggc gtaggcgcgg cggtagtctt cctggccgta gttccgccgg
     2521 tgcgccggcg gcccgtcgac tccggcatcc ggcatgtcca cggtcccgta gaagaacgcg
     2581 ccgaagtgca tctccacctc cagggcccgc gcgttggcga gcgacgtgga caccgcccgt
     2641 gtcgtgagca cgtcgcgcgc gcgatgccgt gcctcggaga tacaccagga ccgccgagca
     2701 atgcaacacg tcgccgtccg agcgcctgcc gggcgcgatc tcgctggtgc gatgcgcggc
     2761 gcgtgccggc gcaccagccg ggtggcgcgc agctcggcga ggaggtgcgc ggccgcccag
     2821 ccgccggggt gcggcccgcg gacggctggc tacgtcttcg agccgaggaa gtcgcggatc
     2881 gtccgggcca ccgtctcggg ctgctccagg tgcggggcgt gcccggcgcc ctccaccgtc
     2941 tccgcgcgcg cggcgggcag gcgccgcgcg aactcgtcgg cgtacaccgg cggcaccaga
     3001 cgatcctcct tgccccacac gagcaaggtg ggtgccgtga cgcggtgaat gcgcttcttc
     3061 agccccttgt cggggatcgg ccagatgaac ttgcccgtgg tccccatcgc ccacatgagg
     3121 cggacccggg ccgcggccgc cgtctccgga tcgtgcgcgt cgccgaacag ccggcgcgcg
     3181 gcctcgccgt caggatcgcg gaagatgtgg gcgcgcatgc gcgacggctc gagcgtcatc
     3241 cagttcacca cggggtcgga gtcgcgccag aagccgagcg ggtcgatgag gaccagtcgg
     3301 cgggcgcggc cggggtacga ggcggcgaac tcgcacgcca ccatcccgcc gaacgagtga
     3361 cccacgagcg ccgcgcgctc gagcccgagc gccgccatca gctcgtcgta gcagaggacg
     3421 aggtcccaca gtccgtcgag atggtagacg tcgtccggag cccccggcgt cgtgcccggg
     3481 tgctcgggcg cgtacacggt gaagctgcgc gccagctcgt cgagaaacgg gtcccacgtg
     3541 aggccccagg gaccatggaa gaagacgagc gccggaccgt cgccttcgga cagcaccctg
     3601 atgcggacct tgccctgcca gacggcgagc gtccgctcgc tcatgcgccg cctgccgcga
     3661 ccccggccac cgccggcgcc ggcgctgcgc gcagccgctc gggccaccag tggttcaccc
     3721 agccctcgtc gtcccaggtg gtccggagat ggggcagcac ctcgccggcg aagcggtcga
     3781 tgttttccag cgccagctcg tgcggcatgg agccgatctg caggagcacc atgaggttgc
     3841 cgacgcgtag gcccttgatc acctcttcct cgagccggtc gcggacggtg gcagggctgc
     3901 cagcgatgac gtagcccttg tccacgaagt cgcggtagcg cagctccttc gggttcacgg
     3961 cgtaacggat gggattcttc gtgctcgcca cgaggctccg gtagtcctgg ttgccgggcg
     4021 gcgagaacca cgggccgggc acgtgcaggc acttgtgata gaagtactcg acgtgccgcg
     4081 cgtatttctc ctcggcgcgg gcgtcgctct cggcgacggc cacgagctgc aggaaccccg
     4141 cgcggtacgg gttgggatcg cgccccttgg cggcgacgac gttccagtag ccctgcatca
     4201 tcgtcctcgc cgaccgtgca cccgagtagc tcagaaagca gtagcagacg tcgtgctcga
     4261 cggcgaactc gaaggtgctg atgctccccg agcccggcac ccacacgggc ggatgcggct
     4321 gctggatggg gcgcggccag aggttcacgt tcgcgagctg gtagtaccgg ccgttccacg
     4381 cgaagatctc gcgggcctgc caggccttga gcgtcagcac gaaggcctcg cggtagcgct
     4441 cgcgatgctc catgggcgtg atgccgtagc agaggttcac gtccatcgga ctgccgaggg
     4501 gcagccccgc cacgagccgc ccgccgctga tgcagtcgag catcgcgtat tcctcggcca
     4561 cgcggatggg cggattcgag gtgggaagcg tcgcgcccat ctggacgatc gcgacgttcg
     4621 tcccgttcgt gagcttggcc aggatgccgc ccatgaggtt cgggctcggc atgaagccgt
     4681 aggcgttctg gtggtgctcg ttggtgcaga gaccgtcgaa gccggcacgt gcggcatgga
     4741 gcagctcgtc cagcgtccag ttgtagtact gtccgactcg cccggcgtcg gccagctccc
     4801 accagggcgg cgtcacccac acggactcgt agcgctgctc gaagtcatcg ggcagctcgc
     4861 ggtgcggcat caggtggaac gcgctgatct tcatgggggc ctccggagcg gagcctacac
     4921 cgccggacgg tcgcccgtcg acggccaccg gacctcggac gcccgtcatc ggccggcgcg
     4981 gcatccgcgc gcggcgtcct ctccgcctca ccccgcgcac gggcgcccgc cgtgccccgg
     5041 cgaggctccg ggtcgggcgg tgccggcgag gcgcccatgg atgcgggcgc ggcgtgtcgg
     5101 gccgaatcag atcagaggct cccgcgccgg gggcgcgcga gccagaagca cgccgagccc
     5161 agcacgcaga agccgacggc gatcaggaag gcgacgcgat agctgccggt cacgtcgtgg
     5221 acggcgccgg cgtaccacgg cgcgataccg ccgccgatgc cactgcccac gctcaggatg
     5281 ccgtagatga cgccgaagcg ccgtcccgga aagagctggg acgcgatcgc cgtgatgatg
     5341 gggccccggg cgccgaagcc gaggccgaag aagatggcgt acgcgtagag ggccgccgcg
     5401 tgcggccaca cctcgagccc gagcaggcag agcgtcccca gcgccgtgca cccgtaggag
     5461 accgtggccg aggcggtgcg gccgatccgg tcggcggcga tgccgaacac gacccgaccc
     5521 accaccgaga gcaggccggt gagcccgaag atgccggcca cgaacaggcg cgggaagccg
     5581 tggtctaccg cgaaggccac ctgatgggtg acgaccgaga aaacggccag cggcgtgcac
     5641 aggtaggcga agaagagcgc ccagaacgcc cgcgtccgca gagcgtcgcc gacttcccgg
     5701 ccggcgccag gcctcgcagc cggctcctcc actccgcgtc ggcctggacc cgtcaccgcg
     5761 accaccggcg cttcccgcac gcccagccag atcaccggca ggagcgtcag cagcgtgccg
     5821 gcgccgagga cgacgtacgc cgtgcgccag ccgtatccgg cgatcagcca ctgggcgagc
     5881 gggccgacgg ccagcacgcc cgcccccatg cccgagaagg ccaggcccag catgctgccg
     5941 cgccgctcga tgaaccaccg cgcgatgagc gccccgctcg gtatccagct cacggcgcac
     6001 accccggccg cggccagcac ccccgtcaac gcgtagagcg accacagcga tccgatccgg
     6061 ctcgacagga cacacgcggt tccgagcaga cacgccccgg cgagcatgac ccggcgcggg
     6121 cccaggcggt ccacgagcat ccccaccccg ggcgacagca ccccctgcac gatcgacgag
     6181 atcgagaacg ccgccgcggt agccccccgc gaccacccga actcctccag cagcgccacg
     6241 aagaacaccg ggaacgagaa gtagagtccg gaggcgatcg ccagcgtgac ccacaccgtg
     6301 gcgaccacga tccacgggcg gagggcgtcg atcagaattc cttcgcgggc gtcacttcaa
     6361 gtgcgtgacg cgaggcgcca cccgctccat caggaggcgc aggctctcgt gatcccaccc
     6421 ggcgtcgtcc gagtcggtcg tgatcgacag cagcgtgccg aagccgccgc tctcctcgtg
     6481 aagctgatgg agcttgtcca c
//
LOCUS       DATIAZ010000074        11560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_17632, whole genome shotgun sequence.
ACCESSION   DATIAZ010000074 DATIAZ010000000
VERSION     DATIAZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17632"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VLF19_02435"
     CDS             complement(<1..299)
                     /locus_tag="VLF19_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92137.1"
                     /translation="MTPPTIAAPAEALRQGTVLVVDDEEGVRASVRAILHGVCDVVEA
                     EDGARALDVLRTHEVDLVMLDQRMPGDPGIEILSRVKALDPTIVVVLATAVHDVRT"
     gene            complement(296..1720)
                     /locus_tag="VLF19_02440"
     CDS             complement(296..1720)
                     /locus_tag="VLF19_02440"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSE92138.1"
                     /translation="MRLLAVLACVLAFAPPVWAQAGPAPAIDPRSVLRALEDAFSAVA
                     DRVTPAVVNVSTVPKKTLASTPDAQERFREFFGEEFFERYFRRRREEPRSSGSGVIVD
                     PSGYILTNNHVIENAQDITVRLSDSRKLTARLVGRDPKTDLAVLKVDALAPLPAAELG
                     DSDNLRVGQWAIAIGNPFGLDRTVTVGIVSATARTRVGVATYESFIQTDASINPGNSG
                     GPLLNLDGKVIGVNTAIVAAGQGIGFSIPINMAKGVMGQLITRGRVVRGWLGIVIQDV
                     TDELSSSFGVREREGVLVADVMRGGPAESAGMRPGDVIVEFDGAPVKEVPDLQRHAAG
                     APPGQRVRLVIVRDGARVPLTVAVGEMPGEEPAAASSQGDEDWGFSVEALGTDPTRRL
                     DLPVGHGVLVTRVAPGSPAERAGLRRGDVILDVKGRPLTDPQALHGVLASLKPGDSVP
                     LYVHRAAGTGGRNEYLVLERPRTP"
     gene            complement(1752..3119)
                     /gene="tilS"
                     /locus_tag="VLF19_02445"
     CDS             complement(1752..3119)
                     /gene="tilS"
                     /locus_tag="VLF19_02445"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSE92139.1"
                     /translation="MPASVLDLVRDTVRRHAMLASGDTVLVALSGGADSTALLLLLSR
                     LAPELGLILHALHVDHGLRPDSVRDAQAALALGERLGVPVEVVAVTVDRDGSLEEAAR
                     RARYAALEAHADRIGATRIAVAHTADDQAETVLMRLLESAGVRGLAGIPPVRGRVVRP
                     VLALRRRQLVAELEGAGLGWVEDPTNRNREFFRNRIRHDVLPLIAAAHDADVVPALAR
                     VAARAREAVDALERLAARELARVATVGADDVLLPRETLAALPRPAAVEVLRQAAARLG
                     SRAPLRAWAHRGLARVLAEPAPRHPFRLGAVTIEASSRLVRVARRGDPPTLTPRALAV
                     PGSVELPEARVVIEARLCDPVGYAVPRAPARAAFDADALALPLTVRGRRQGDRFRPWG
                     GPGERRLKSFMIDVHIPRWERDRLVVVESAGEIAWLAGVRRAALAPITDRTRGVIELS
                     VGPLA"
     gene            complement(3122..3841)
                     /locus_tag="VLF19_02450"
     CDS             complement(3122..3841)
                     /locus_tag="VLF19_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSE92140.1"
                     /translation="MRVRVRLFARYREAAGRERLEVDLPEGGTVEAAWLAIVARHPQL
                     SPYRPYTLFAVGHDYVNPEHSLVQGDELCLFPPVSGGSASEPPPRVARAEPALERGDV
                     YRVVSVPLSPDAIAAAVDDPGAGGVVIFSGVVRNETGGRPVKFLEYEGHVPMAEAKLR
                     EIGESARARWAGIKRVAILHRLGRLEIGESSVLIAVSAAHRAEAFEACRYAIDTLKRT
                     VPIWKKEHFEDGEVWVGLQGG"
     gene            complement(3866..4192)
                     /locus_tag="VLF19_02455"
     CDS             complement(3866..4192)
                     /locus_tag="VLF19_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSE92141.1"
                     /translation="MVTLTETAAKKVTDLRLEEGKPEWGLRVRVVGGGCSGMSYELGW
                     DDQAVEGDTVVESHGVKVYVDQNSAAYLAGSEIDFVDNNMMGAGFAIKNPNVKSSCGC
                     GQSHRF"
     gene            complement(4269..4997)
                     /locus_tag="VLF19_02460"
     CDS             complement(4269..4997)
                     /locus_tag="VLF19_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92142.1"
                     /translation="MTTPGALAGQVAIVTGAGRGIGRAIARAFAREGAAVALVARTRA
                     DLAAAAAEIRDGGGTALALPTDVTQDAAVEALVEQAAAELGRLDVLVTAAGVAAFGPV
                     AAAKPADWDPMIAVNLRAVMVCCRAALGPMLRQRRGTILNVASIAATRAIPGAAAYAA
                     TKAGVLAWSRVLADEVRGEGVRVGVLSPGATDTPLWDAIPGGPDRSRMLRPEDVARAA
                     VLMAALPPNAALEELTLLPAGGIL"
     gene            complement(4994..5575)
                     /gene="folE"
                     /locus_tag="VLF19_02465"
     CDS             complement(4994..5575)
                     /gene="folE"
                     /locus_tag="VLF19_02465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004477634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSE92143.1"
                     /translation="MNDHGAIARLVEQLLKELGEDAYREGLERTPERVEKALRYLTSG
                     YRMNVHEVLNDALFVEEYDEMVVVKDIDVFSLCEHHLLPFIGRCHVAYMPHKKIVGLS
                     KIPRLVEVYARRLQVQERLTTQIAYTLNEVLQPRGVAVVMEAVHLCMVMRGVEKQNSK
                     AVTSAMLGAFRERPETRAEFMELIKTGGRGPQI"
     gene            complement(5572..5973)
                     /locus_tag="VLF19_02470"
     CDS             complement(5572..5973)
                     /locus_tag="VLF19_02470"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSE92144.1"
                     /translation="MMELTRVYHFSAAHRLASPALEPAENARLYGPCAEPHGHNYYLE
                     VTVAGRPDPVTGMLVDVAQVDERVGAVVGRVDHRTLESVCELTGVITTGECLARAFWR
                     LLAPGVGPATLARVTVVETAKNRFEYRGESS"
     gene            complement(5970..6383)
                     /locus_tag="VLF19_02475"
     CDS             complement(5970..6383)
                     /locus_tag="VLF19_02475"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSE92145.1"
                     /translation="MSTPAYSTRRFAFSAGHRYWVDAWSAEENQRVFGRLTVPHGHNY
                     TLDVTVRGPIDDRTGMVIDLSELKRVVGETVVDRFDHADLNADPLFRGRVPTTENIAA
                     AVWDLLAAKLGTDRLWRVRVWEDPTLYVDYGGDGR"
     gene            complement(6380..7378)
                     /locus_tag="VLF19_02480"
     CDS             complement(6380..7378)
                     /locus_tag="VLF19_02480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSE92146.1"
                     /translation="MALDGLRRGLVRLGHEAELRPLRWRTRFHTLDRWLYNAGVALAP
                     PAADVVVGLDLDGFLWARRRDPRMPFVAALKGVIADELANERGLVRVLLGVQARWERA
                     NCARADRVVVPSRYSAGVASAAYAIAPARLAVVPEPIDLAAWRRLFAGVRTARAARPT
                     VLAVARMYPRKRLQDLLEATALLAARIPDVQARIVGAGPEFSRLRADHARLGLGDRVV
                     FLGEVPRSILAVEYVRAHCFCLPTVQEGFGIVFAEAMAAGLPVVACRAAAVPEVVADG
                     HTGLLVEPRSPAALAAALERLLTDDGLRKDLGEEGMRRVESYDLDRVATRFLEAVP"
     gene            complement(7440..8231)
                     /locus_tag="VLF19_02485"
     CDS             complement(7440..8231)
                     /locus_tag="VLF19_02485"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSE92147.1"
                     /translation="MSEGRDILVRHDGAVATVVFNRPRMRNAISLAMWGEIARVTEGL
                     AKDDSVRAIVYRGAGTDAFASGADISEFEENRKDTASALRYNSQTSAAYRGIRECPKP
                     TVAMIYGYCMGGAMAVAMACDLRFAAEGSRFGIPAARLSIIYGADAVGQLVDLVGPAY
                     AKDILYSARTVGDREALAMGFIQRLVPAGELEAYTYEYLGKVVDNAPISVRGAKATVE
                     GYLGGMTAEHRERLGRLQLETFESEDYKEGTRAFLEKRPPRFQGR"
     gene            complement(8257..8331)
                     /locus_tag="VLF19_02490"
     tRNA            complement(8257..8331)
                     /locus_tag="VLF19_02490"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8297..8299),aa:Met,seq:cat)
     gene            8509..8775
                     /locus_tag="VLF19_02495"
     CDS             8509..8775
                     /locus_tag="VLF19_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92148.1"
                     /translation="MQFRNHPWYDDAELRQTPTNGDEDADEVDDDEESDDEDDDEFED
                     EFDDDEFEDDDDDDLEDDGDVVADEEDEFDGDGENAEDEDGEDE"
     gene            8808..9527
                     /locus_tag="VLF19_02500"
     CDS             8808..9527
                     /locus_tag="VLF19_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HSE92149.1"
                     /translation="MALFSGDGWLFLLRWIHFLSGVTWIGLLYYFNFVQTPFFASAEA
                     PVRSGMVAGGLVERALWWFRWGAMFTFLSGWLIVFDRMGRLGLAGFFDTSYGWAIFYG
                     GMLGSLMWANVWFVIWPAQQVVIKSAQQVKQGGQAIAEAAARGQRGGFASRTNTMLSI
                     PMLFFMGAASHLTIFSATTGGAKLGALVLLAIVLIAAEVNALVGTTGSGKKMLSTLKG
                     TFWGGFVLTAILYLGLELLFP"
     gene            complement(9629..9949)
                     /locus_tag="VLF19_02505"
     CDS             complement(9629..9949)
                     /locus_tag="VLF19_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSE92150.1"
                     /translation="MRSPRLLLGLVTLVMLGGCGSEPGLSPEAERGRQVYLGQCAVCH
                     GQDPAQAGPLGPPVKGASRELLEGKVLRGAYPPGYTPKRSTTVMPPLPALAPEIPALA
                     EYLR"
     gene            complement(10055..10384)
                     /locus_tag="VLF19_02510"
     CDS             complement(10055..10384)
                     /locus_tag="VLF19_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92151.1"
                     /translation="MVDDEVDVQEVVVEVLSEGGYQVEAVGSAAEALEALGRSAWDLV
                     ITDVRLGDQPGPELAREIARRRPALSNRIIFVTGDVAAALDHAPVIRKPFSLDDVLDA
                     VGERLSS"
     gene            complement(10408..10707)
                     /locus_tag="VLF19_02515"
     CDS             complement(10408..10707)
                     /locus_tag="VLF19_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92152.1"
                     /translation="MNGMLVDSNVYYLPVAPVAEAIAEREWPTMGTRLRNAWWRLRLA
                     LAEVRGILRRPRRFQPDAHAEFAELEVVRVSRRSRPARIIDLDSARRRLRPEPRA"
     gene            complement(10704..11108)
                     /locus_tag="VLF19_02520"
     CDS             complement(10704..11108)
                     /locus_tag="VLF19_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92153.1"
                     /translation="MALFAARPMSLDTPVDSLDPRPSGITLWRPEIVLPEGLVLAERL
                     RVTIAEPAQRPEAVRAQIDATRSLLTSLAEARQTLAGRATASEVRALGADSERNRVAA
                     MIETYRVKTALEDVLHGTIRQMSVLMGERGRR"
     gene            complement(11268..>11560)
                     /locus_tag="VLF19_02525"
     CDS             complement(11268..>11560)
                     /locus_tag="VLF19_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSE92154.1"
                     /translation="PGNIRELRNMVERLMILHEGDEVQANELPVELSTQSRPPTEPAD
                     AALVPLAEMERRYVERVLASTGWNKAQAARVLDIDIKTLNKKIRDFNLTRLA"
BASE COUNT         1677 a         4185 c         4082 g         1616 t
ORIGIN      
        1 gtgcgcacgt cgtgcacggc ggtggcgagc acgacgacga tggtggggtc gagggccttc
       61 acccgcgaca ggatctcgat gccgggatcc cctggcatcc gctgatccag catgacgagg
      121 tccacctcgt gggtcctcag cacgtcgagc gcgcgcgcgc cgtcctcggc ctccaccacg
      181 tcgcagacgc cgtggaggat cgctcggacc gaggcgcgga cgccttcctc gtcgtccacg
      241 acgaggacgg taccctgccg cagcgcctcg gccggcgcgg cgatcgtggg cggcgtcacg
      301 gcgtgcgggg ccgttccagc accaggtact cgttgcggcc gccggttccc gcggcgcggt
      361 gcacgtagag ggggacggag tcgccaggct tcagcgaagc gaggaccccg tggagcgcct
      421 gggggtccgt gagggggcgg cccttgacgt cgaggatcac gtcaccgcgg cgcaggccgg
      481 cccgttccgc cgggcttccg ggcgcgacgc gcgtgaccag cacgccgtgg cccacgggca
      541 ggtcgagccg gcgcgtggga tcggtcccca gcgcctcgac gctgaagccc cagtcctcgt
      601 cgccctggct cgacgccgcc gcaggctcct cgcccggcat ctcgcccacg gcgacggtga
      661 gggggacgcg cgccccgtcg cggacgatca cgagccgcac gcgctgcccc ggcggggcgc
      721 ccgccgcatg tcgctgcagg tctggcacct ccttgacggg cgcaccatcg aactcgacga
      781 tcacgtcgcc ggggcgcatc ccggccgact cggccggacc tccgcgcatg acgtccgcga
      841 ccagcacacc ctcgcgctcg cggacgccaa agctcgacga cagctcgtcg gtcacgtcct
      901 ggatgacgat gccgagccat ccacgtacga cgcgcccgcg cgtgatgagc tggcccatga
      961 cgcccttcgc catgttgatc gggatcgaga agccgatccc ctgcccggcg gcgacgatcg
     1021 ccgtgttcac tccgatgacc ttgccgtcca ggttcaggag cgggccgccg gaattgcccg
     1081 ggttgatgga ggcgtcggtc tggatgaagg actcgtaggt ggcgacgccg acccgcgtgc
     1141 gggccgtggc cgacacgata ccgacggtga ccgtgcggtc caggccgaag gggttgccga
     1201 tggcgatcgc ccactggccc acccgcaggt tgtcggagtc gcccagctcg gccgccggca
     1261 gcggggccag cgcgtcgacc ttcagcaccg ccagatcggt cttggggtcg cggccgacca
     1321 ggcgggcggt gagcttccgg gagtccgaga gacgcacggt gatgtcctga gcgttctcga
     1381 tgacgtggtt gttcgtcaag atgtatcctg aaggatccac gatcacgccg gagccgctcg
     1441 atcggggctc ctcgcgacgc cggcggaagt agcgctcgaa gaactcctct ccgaagaact
     1501 cgcggaagcg ctcttgcgcg tcgggcgtgg acgccagcgt cttcttcggc acggtgctca
     1561 cgttgacgac cgcgggcgtg acgcggtcgg cgacggccga gaaggcgtcc tcgagcgcgc
     1621 gcagcacgct cctgggatcg atcgcggggg ccgggccggc ctgcgcccac accggaggcg
     1681 cgaaggcgag cacgcacgca agaaccgcga ggagtctcat cgccgcgggc attctacggc
     1741 ccgcgggtgc cctacgccag gggcccgacg ctcagctcga tcacgccgcg cgtgcggtcg
     1801 gtgatcggcg cgagcgcggc gcgccgcacg ccggcgagcc aggcgatctc gcccgcggac
     1861 tcgacgacga cgaggcggtc gcgctcccat cgagggatgt ggacgtcgat catgaacgac
     1921 ttgagtcgcc gctctccggg gccaccccat ggccggaacc ggtcgccctg gcgccgcccg
     1981 cgcacggtga gcggcagcgc gagcgcatcg gcgtcgaacg ccgcgcgcgc gggcgcacgc
     2041 ggcaccgcgt agccgacggg gtcgcagagc ctcgcctcga tgacgacacg cgcctcgggc
     2101 agctccacgc ttcccgggac ggcgagcgcg cgcggcgtca gcgtgggcgg gtcgccccgc
     2161 cgcgccacgc ggacgagccg gctgctcgcc tcgatcgtca ccgcgccgag ccggaacggg
     2221 tggcgcgggg ccggctcggc gagcacgcgc gcgaggccgc ggtgcgccca ggcgcgcagc
     2281 ggcgcgcggc tgcccaggcg ggcggcggcc tgccgcagga cctccaccgc cgccggccgc
     2341 ggcagcgccg cgagcgtctc gcggggcagc aggacgtcgt cggcgccgac ggtggccacg
     2401 cgcgcgagtt cgcgggcggc gagccgctcc agcgcgtcga ccgcctcccg cgcccgcgcg
     2461 gcgacgcgag ccagcgccgg cacgacgtcg gcgtcgtggg cggcggcgat gagcggcagc
     2521 acgtcgtgac ggatccggtt acggaagaac tcgcggttgc gattcgtcgg atcctcgacc
     2581 cagccgagtc cggcgccctc cagctcggcg acgagctggc ggcggcgcag cgcgaggacg
     2641 ggccgcacga cacggccgcg cacgggcggg atgccggcga ggccgcggac gccggcgctc
     2701 tcgaggaggc gcatcagcac cgtctcggcc tggtcgtctg ccgtgtgggc gaccgcgatg
     2761 cgggtcgcgc cgatgcggtc ggcgtgggcc tcgagggccg cgtaacgcgc gcggcgcgcg
     2821 gcctcctcga gcgagccgtc ccggtcaacc gtgaccgcga cgacctcgac gggcacgccc
     2881 agacgctcgc cgagcgcaag cgccgcctgc gcgtcgcgca ccgagtcggg gcgaaggccg
     2941 tggtcgacgt gcaaggcgtg gagaatgagc ccgagctcgg gcgccagacg ggagagcagc
     3001 aggaggaggg ccgtggaatc ggcgcctccg gacagcgcca ccagcacggt gtccccggac
     3061 gccagcatcg cgtggcggcg aacggtgtcg cggacgaggt cgagaacgga ggcgggcacc
     3121 gctacccgcc ctgcagtccc acccagacct cgccgtcctc gaagtgctcc ttcttccaga
     3181 tggggacggt ccgcttcagc gtgtcgatgg cgtagcggca ggcctcgaag gcctccgcgc
     3241 ggtgggcggc ggacaccgcg atgaggacgc tcgactcgcc gatctcgagc cgaccgagtc
     3301 gatggaggat ggccacacgc ttgatgccgg cccagcgagc gcgcgccgac tccccgatct
     3361 cccgcagctt ggcctcggcc atcggcacgt gcccttcgta ctcgaggaac ttcaccggcc
     3421 gtcccccggt ctcgttccgc accacccccg agaagatcac gactccaccc gcccccggat
     3481 cgtccaccgc cgccgcgatc gcatcgggcg acagcggcac actcaccacg cggtagacat
     3541 caccgcgctc gagcgcgggc tccgcccgcg caacccgagg aggaggctcc gaggcgctcc
     3601 ctccgctcac gggcgggaac aggcagagct cgtcgccctg gaccagcgag tgctcggggt
     3661 tgacgtagtc gtgtcccacg gcgaagagcg tgtacggccg gtagggcgag agctgaggat
     3721 gacgggcgac gatggccagc cacgcagcct cgaccgtgcc gccctcggga agatcgacct
     3781 cgagccgctc ccgcccggcg gcctcacggt accgggcgaa gagacggact cgaacacgca
     3841 cggcgttacc tcgacagcga accgactaga agcggtgcga ctgcccgcac ccgcagctgg
     3901 acttcacgtt cgggttcttg atcgcgaagc cggcacccat catgttgttg tcgacgaagt
     3961 cgatctcgct gcccgcgagg tacgccgcgc tgttctggtc cacgtagacc ttgacgccgt
     4021 gggactcgac gacagtgtcg ccctcgaccg cctggtcgtc ccaccccagc tcgtacgaca
     4081 tgcccgagca tccgcctccc acgacccgga cgcgcaggcc ccactcgggc ttgccctctt
     4141 ccaggcggag atccgtgacc ttcttggcgg ccgtttccgt cagcgtgacc atgcgcttcc
     4201 ctccgtgccc ccggacgccg ctggtgacgc acgccgagtg ctcgatcggt gtcgatatgt
     4261 ggatgatatc acaggattcc gcccgcgggc aggagggtca gctcctcgag cgcggcgttc
     4321 ggcggtagtg cggccatgag gaccgcggcg cgcgccacgt cctcggggcg gagcatgcgg
     4381 ctccgatccg gcccgccggg gatcgcgtcc cacaggggcg tgtccgtcgc cccgggcgac
     4441 aggaccccca cccgcacccc ctcgccgcga acctcgtcgg cgagcacccg gctccacgcc
     4501 aacacgccgg ccttggtcgc ggcgtaggcc gccgcgcccg ggatcgcccg cgtggcggcg
     4561 atcgaggcca cgttgaggat ggtgccgcgg cgctggcgca gcatcggacc gagcgcggcc
     4621 cggcagcaca ccatcacggc gcgcaggttt acggcgatca tcgggtccca gtcggcgggc
     4681 ttggccgcgg ccacggggcc gaacgccgcg acccccgcgg ccgtcaccag cacgtcgagc
     4741 cgcccgagct ccgccgccgc ctgctcgacg agcgcctcca ccgccgcgtc ctgcgtcacg
     4801 tccgtgggga gcgccagcgc ggtaccaccc ccgtcgcgga tctcggcggc cgccgcggcc
     4861 aggtccgcgc gcgtgcgcgc caccagcgcc accgcggcgc cctcacgggc gaaggcgcgg
     4921 gcgatggcgc gcccgatgcc gcgcccggcc ccggtgacga tcgcgacctg cccggcgagc
     4981 gcgcccggcg tcgtcaaatc tggggccctc gcccgccggt cttgatcagc tccatgaact
     5041 ccgcgcgagt ctcgggccgc tcccggaagg cgcccagcat cgccgaagtc acggccttgc
     5101 tgttctgctt ctccaccccg cgcatcacca tgcagaggtg gaccgcctcc atgaccacgg
     5161 cgacgccgcg cggctgcagc acctcgttca gcgtgtaggc gatctgggtc gtgagccgct
     5221 cctgcacctg caaccgccgc gcgtacacct cgacgagccg cgggatcttc gacagcccca
     5281 cgatcttctt gtgcggcatg taggcgacgt ggcagcgacc gatgaagggc aggaggtgat
     5341 gctcgcagag gctgaacacg tcgatgtcct tcacgacgac catctcgtcg tactcctcga
     5401 cgaagagggc gtcgttgagg acctcgtgga cgttcatgcg gtagcccgac gtgagatagc
     5461 gcagcgcctt ctcgacccgc tccggtgtgc gctccaggcc ctcgcggtac gcgtcctcgc
     5521 ccagctcctt gaggagctgc tcgacgaggc gcgcgatcgc gccgtggtcg ttcatgacga
     5581 ttccccccgg tactcgaagc ggttcttggc ggtctcgacg acggtcaccc gcgcgagcgt
     5641 tgccgggccc acgcccggcg ccagcagccg ccagaaggca cgggccaggc actctcccgt
     5701 cgtgatcacg ccggtcagct cgcacacgct ctccagcgtc cggtggtcga cgcggccgac
     5761 gaccgcgccc acgcgctcgt cgacctgcgc gacgtccacc agcatccccg tcacgggatc
     5821 ggggcggccg gccacggtga cctccaggta gtagttgtgc ccgtgcggtt ccgcacacgg
     5881 cccgtagagc cgcgcgttct ccgccggctc gagcgccggg ctcgcgaggc ggtgggccgc
     5941 gctgaagtgg tacacgcggg tcaactccat catcgcccgt cgccgccgta atcgacgtag
     6001 agcgtcgggt cttcccagac gcgcacgcgc cagagccggt cggtgccgag cttggccgcc
     6061 agcaggtccc agaccgcggc ggcgatgttc tcggtcgtcg gcacccggcc gcggaagagc
     6121 gggtcggcgt tcaggtcggc gtggtcgaag cggtccacca cggtctcgcc gacgacgcgc
     6181 ttgagctccg agagatcgat gaccatcccg gtgcggtcgt cgatcgggcc gcggacggtg
     6241 acgtccagcg tgtagttgtg gccgtggggc accgtgaggc ggccgaacac gcgctggttc
     6301 tcctcggccg accatgcgtc cacccagtac cggtggccgg ccgagaacgc gaaccgccgc
     6361 gtgctgtacg cgggcgtgct cacggaacgg cttccaggaa tcgtgtcgcc acgcggtcca
     6421 ggtcgtagct ctccacgcgc cgcatcccct cttcccccag gtccttgcgc agcccgtcgt
     6481 ccgtcaggag ccgctccagc gccgccgcga gggccgccgg gctgcggggc tccaccagca
     6541 gccccgtgtg cccatcggcg accacctcgg ggactgccgc cgcccggcac gcgacgacgg
     6601 ggagtcctgc ggccatggcc tcggcgaaca cgatcccgaa gccttcctgc acggtcggca
     6661 ggcagaagca gtgcgcccgg acatattcta cggccaggat cgagcgcggg acctcgccga
     6721 ggaagaccac gcgatcgccc agcccgaggc gcgcgtgatc cgctcgcagg cgcgagaatt
     6781 ccggccccgc ccctacgatg cgggcctgga cgtcggggat tcgggcggcg agcagcgccg
     6841 tcgcctccag caggtcttgc agccgcttcc gggggtacat gcgcgccacg gcgagcaccg
     6901 tcggccgcgc ggcacgcgcc gtccgcacgc cggcgaagag ccgccgccag gcggcgaggt
     6961 cgatcggctc gggcacgacc gcgaggcgcg ccggagcgat ggcgtacgcg gcggaggcga
     7021 cgcccgccga gtagcggctc gggaccacga cgcggtcggc gcgggcgcag ttggcgcgct
     7081 cccagcgcgc ctgcacgccg agcagcacac gcacgagtcc gcgctcgttc gcgagctcat
     7141 cggcgatcac gcccttcagc gccgccacga acggcatcct cgggtcccga cggcgtgccc
     7201 agaggaatcc gtcgagatcg aggccgacga cgacatcggc ggcgggcggc gccagtgcca
     7261 cgccggcgtt gtagagccag cggtcgaggg tatggaaccg ggtccgccac cggagcgggc
     7321 gcagctcggc ctcgtgcccg agccggacga gcccgcgccg gagaccgtcg agggccacga
     7381 acgtcccgct cccctccgtc gggctccccg gagtggagct caggaacgcg aggcgcaatc
     7441 tagcgtccct ggaacctcgg cgggcgcttc tcgaggaagg cgcgcgtgcc ttccttgtag
     7501 tcctcgctct cgaacgtctc gagctgcagg cggcccagac gctcgcggtg ctcggcggtc
     7561 atgccgccga ggtacccttc cacggtcgcc ttggccccgc gcaccgagat cggcgcgttg
     7621 tccacgacct tgccgaggta ctcgtaggtg tacgcctcca gctcgcccgc gggcaccagc
     7681 cgctggatga agcccatggc gagcgcctcg cggtcgccga ccgtccgcgc ggagtagagg
     7741 atgtccttgg cgtacgcggg ccccacgagg tccacgagct gccccaccgc gtcggcgccg
     7801 tagatgatgg acagccgcgc cgcgggaatg ccgaaccggg acccctcggc ggcgaaacgg
     7861 agatcacagg ccatcgccac cgccatggcc ccgcccatgc agtagccgta gatcatcgcg
     7921 acggtgggct tcgggcactc gcggatcccg cgatacgcgg cggaggtctg gctgttgtag
     7981 cggagcgcgc tcgccgtgtc cttccggttc tcctcgaact ccgagatgtc cgcgccggaa
     8041 gcgaaggcgt cggtgccggc gccgcggtag acgatcgccc gtacggagtc gtccttggcg
     8101 agcccctcgg tgacccgcgc gatctcgccc cacatcgcga ggctgatggc gttccgcatg
     8161 cgcggacggt tgaagaccac cgtggcgacg gcgccgtcgt gccggacgag gatgtccctg
     8221 ccttcgctca tgtgtccacc ccggtgccgg gagacttggt ggcggtggga ggaattgaac
     8281 ctccgacacg gggcttatga gacccctgct ctgccgctga gctacaccgc cctggcaagc
     8341 gaacgacgag tcagtgtacc gcaacggcgc gcggcccgca gggccgttcc tcggtcccca
     8401 aatggtcctg gacacatgtc ccgttttgtt ataatccgcc ccgagcatcc caacagtagc
     8461 gactccggct actcaggcca ggccgggcgc cggctcggag gacctcagat gcagttccgg
     8521 aatcacccct ggtatgacga cgcggagctt cgtcagacac ccaccaacgg cgacgaggac
     8581 gccgacgagg tggacgacga cgaggaatcc gacgacgagg acgacgacga gttcgaggac
     8641 gagttcgacg acgacgagtt cgaggacgac gacgacgatg acctggagga cgacggagac
     8701 gtggtcgccg acgaggaaga cgagttcgac ggcgacggcg agaacgccga ggacgaggac
     8761 ggggaggacg agtagtccat cgcactaaca ggaggagggc caggctgatg gcactgttca
     8821 gtggagatgg ttggctgttc ctgctgcgat ggatccactt cctctcgggc gtcacctgga
     8881 tcggtctgct gtactacttc aacttcgtcc agacgccctt cttcgcctcg gccgaggcgc
     8941 ccgtgcgctc agggatggtg gcggggggac tcgtcgagcg ggccctctgg tggttccggt
     9001 ggggcgcgat gttcaccttc ctcagcggct ggctcatcgt cttcgaccgg atgggccggc
     9061 tcggcctcgc cggcttcttc gacacctcgt acgggtgggc gatcttctac ggcggcatgc
     9121 tcggcagcct catgtgggcc aacgtctggt tcgtgatctg gccggcccag caggtggtca
     9181 tcaagtcggc gcagcaggtg aagcagggcg gccaggccat cgccgaggcc gcggcgcgcg
     9241 gccagcgcgg cggcttcgcg tcgcgcacca acacgatgct ctcgatcccg atgctgttct
     9301 tcatgggcgc ggcgagccac ctcacgatct tctcggccac cacgggaggc gcgaagctgg
     9361 gcgccctcgt gctgctggcc atcgtcttga tcgcggccga ggtgaacgcg ctcgtcggca
     9421 ccacggggtc gggcaagaag atgctgagca cgctgaaggg gacgttctgg ggaggcttcg
     9481 tcctcaccgc gatcctctac ctcggccttg agctgctctt cccctagacc aggcgcctgc
     9541 gggatatcga gacgtgccag ggctccgcgg ggcgcgccga acatcggggc ctctcggggc
     9601 ccatgccact acagggccgc gtcgggcttc atctcaagta ctcggcgagc gcggggatct
     9661 ccggcgccag cgcgggcagc ggcggcatga ccgtggtgct tcgcttcggc gtgtagccgg
     9721 gtggataggc ccctcgcagt accttccctt cgagcaattc gcgcgaggcg cccttcaccg
     9781 gtggcccgag cggcccggcc tgcgccgggt cctgtccatg gcaaaccgca cactgcccca
     9841 gatagacctg ccgcccccgc tccgcctcgg ggctcaaccc cggctcggac ccgcaccccc
     9901 cgagcatcac gagcgtcacg agtccgagca gaaggcgtgg ggagcgcacg gccgcatatt
     9961 gtagcggcgc gatgaaactg ccggtgccag aaactcggct gaatcaccgc cttcgagcgc
    10021 cggcgttgcc ggcgcggcga cgccccctcc gggactagga ggaaaggcgt tcgcccaccg
    10081 cgtcgagcac gtcgtcgaga ctgaacggct tgcggatgac cggggcgtgg tccagcgccg
    10141 ccgcaacgtc gccggtgacg aagatgatgc ggttggagag cgcgggccgc cgccgcgcga
    10201 tctcgcgggc cagctccggc cccggctggt cgccgagacg cacgtcagtg atcacgagat
    10261 cccaggcgct gcgcccgagc gcctccagcg cctccgccgc ggaaccgacg gcctcgacct
    10321 ggtagccgcc ttcgctcaac acctcgacca cgacctcctg cacgtcgacc tcgtcgtcga
    10381 ccacgaggat gcgcgcggtc ggagcgctca cgcgcgcggc tcgggacgca ggcgccggcg
    10441 ggcgctgtcc aggtcgatga tacgggcggg acggctgcgg cgggacaccc gcacgacctc
    10501 cagctccgcg aattccgcgt gggcgtcggg ctggaagcgc cgcggacgcc gcagaatgcc
    10561 ccgcacctcc gcgagagcga gccgcagccg ccaccaggcg ttccgcagcc gcgtgcccat
    10621 ggtgggccac tcccgctcgg cgatcgcttc ggccaccggg gcgaccggca ggtagtacac
    10681 gttggagtcc acgagcattc cgttcatcgg cgtccccgct cccccatcaa gacgctcatc
    10741 tggcggatcg tcccatgcag gacgtcctcg agcgcggtct tgaccctgta tgtctcgatc
    10801 atcgcggcca cccggttccg ctccgaatcg gcgccaagcg cccgcacctc cgaggcggtc
    10861 gcgcggcccg cgagcgtctg gcgcgcctcg gccagcgacg tgagcagcga ccgcgtggcg
    10921 tcgatctggg cgcgcaccgc ctccggtcgc tgcgccggct cggcgatcgt gacgcggagt
    10981 cgctcggcca gcaccaggcc ctctggaaga acgatctccg gccgccacaa cgtgatgccg
    11041 gagggccgcg gatcgaggct gtcgacgggc gtgtccaggc tcatcgggcg ggcggcgaat
    11101 agtgccatga tacgagcctt gtaggcaacc gaggtgccac tccatccgaa ctgtaaagac
    11161 ttgattccaa gtcgtttgtt agagtttcac agccgggcgg gtagggagga cctcctggga
    11221 gaagggaatt tctcctgggt cgctttcggg gggcaggccg gccggcttca ggccagcctg
    11281 gtgaggttga agtcccggat cttcttgttg agggtcttga tgtcgatgtc gagaacgcgc
    11341 gcggcctgcg ccttgttcca gccggtggac gcgagcacgc gctcgacgta gcggcgctcc
    11401 atctcggcga gtggtaccag cgcggcgtcg gccggctcgg tggggggccg gctctgcgtg
    11461 gacagctcca cgggcagctc gttggcctgc acctcgtcgc cctcgtggag gatcatcagg
    11521 cgctcgacca tgttgcgaag ctcgcgaatg ttgcctggcc
//
LOCUS       DATIAZ010000075         5748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1464588, whole genome shotgun sequence.
ACCESSION   DATIAZ010000075 DATIAZ010000000
VERSION     DATIAZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464588"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VLF19_02530"
     CDS             <1..831
                     /locus_tag="VLF19_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HSE92155.1"
                     /translation="AMIASGVMAGKIALEDAYNARDRDGIRLVDELERIGYLGPEPCV
                     PRPFRAYLELHIEQGPFLEESRLSVGVVEGIVAISWSRLAIHGVQDHAGPTPMRIRHD
                     ALVAAAAVVTGVRSIAGQLGGDAVTTVGTLDVVPNIVNAIPGRVRLSIDVRDPRTEAL
                     DRARAMIEAVVREACEREGVRYELEHYWRVPATPFDRAVVDAVERAAKASGARYRRMT
                     SGAGHDAQYMAALGPTGMVFVPSRDGRSHCEEEFTPMDDIEHGANTLLLTALDLAGRA
                     "
     gene            851..1135
                     /locus_tag="VLF19_02535"
     CDS             851..1135
                     /locus_tag="VLF19_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92156.1"
                     /translation="MRIDAYVKSVLTVIAVALVAIALNPWLAVLTPRGVQAQTVTPKY
                     EITLLKAWGKIVGFSNQNLLMEAPDGTLRLVDLEGKAPEYPKVKSYMRWQ"
     gene            complement(1315..2094)
                     /locus_tag="VLF19_02540"
     CDS             complement(1315..2094)
                     /locus_tag="VLF19_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSE92157.1"
                     /translation="MTDRGVHLVVASYNIHRGVGLDRRRDLERVADVIGEMTPDVIGL
                     QEVVREDGHVHADQASYLASRLGMALVMCVTRAHGQGTYGNAVLTRLPVLGSSSCDLS
                     RDGREPRACLRVDLGANGRTLHVFNCHFGLGLRERRAQAETLAGFIRDSAALAGPRVL
                     MGDFNEWHPGPVSRGLKREFSSPMRRMRRTHPAMFPLFKLDRIYWDVELEGETFDVHR
                     SRLARVASDHLPVVARLRVRGRAGAGAYHPYVTGDALPPPE"
     gene            complement(2087..3715)
                     /locus_tag="VLF19_02545"
     CDS             complement(2087..3715)
                     /locus_tag="VLF19_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSE92158.1"
                     /translation="MALDELVLALQRRIDRAIRRLRLGPAPERGARKRLLVVQIDGLA
                     RDVLERALAEGRMPFLERLLRRERWRMAPMSVGLPTSTPAFQMGVFYGVRPDIPGFHY
                     HDKRRRSDIYFPRGGDAAHVEATQAAGRRGILEGGSAYACVFTGGAVNNLFNFATLKR
                     PTGHGLLRVISAFVVLTWVVVKCLALTGVELTRALLRFVADPVGETRRGWKWLALKIG
                     LSVWLRELFTLAVSRDLYAGAPAVYVNYLDYDVFAHAFGPRHRRALRSLRHVDSSLRQ
                     LWRVIGRVPGHRYEMYVLSDHGQTTCRSYRKVSGGQPIERVFFEEFFRPAGMVEVGPA
                     APRGRRLAAGIEAFRSRRAPGMFQRFANYLERDFPKLMGELPESRERDGVRVIGAGPN
                     AFVYFLDHPDPLTIDDIDERFPGLADEIGRSRGVGFVLARSRPGPLCLWRGKRYALDG
                     EAVGPFAGRADWPLVAAGVRDLMAMRCAGDLVIYGHDSPDGNISYVAEIGAHAGPSPE
                     EMECFVLCRPGIGLPEPLTHPTQLYPHFAAYQRD"
     gene            3745..>5748
                     /locus_tag="VLF19_02550"
     CDS             3745..>5748
                     /locus_tag="VLF19_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92159.1"
                     /translation="MPAAVHPLLEDVRRFVDAEVIPAASALEHADAYPHALVARMRAL
                     GLFGALVPRAWGGLGLDVTTYARVIAELCRGWMSLAGVINSHTMAALIVLHHGTEAQR
                     ARLLPRFASGAARGGLCLTEPHAGSDVQAIRTVARRRGEDYLLNGTKMFVTNGREGNT
                     FALLAVTDSAAAPRHRGMSCFIVEKGDEPEARGLTVVKSISKLGYKGVDTAELLFEDF
                     AVPGASLVGGVEGRGFKHVMSGLETGRINIAARAVGVGQAAWDAARADGRRREPPPPV
                     LADMAARLETARLITVWAAGMKDRAERCDLEAGMAKLHASETAQRLAEDALRVGGEAA
                     ALASSVVERLYRDTPLMIIGEGTNEIQRTLIARQLLERHGERLGALTGRDAESEERRQ
                     IVLAVRQFVEKAVVPVAWEGERAARYPEDLVRDLAHLGALGALAPPALGGLGLDLRTY
                     AMLVEELARGWTAVAAIVAGHATVTWAITQHGVRHLREHWLPPMTRGEILGAVALDGD
                     VTARAEGGTVILHGRTALVDAAPRAGVHLVRARLGGGRSVLAVVPRGTPGASVGAVQP
                     TLGARGLEVAPVDFDGARVSASAVLEAGALRAHPTGDASVSALGLARLGLAATAVGLA
                     QAAFEAALRYSQQRSAFGQAICHHQAIQLKLADMATAITAARLL"
BASE COUNT          830 a         2069 c         2116 g          733 t
ORIGIN      
        1 gcgatgatcg ccagcggcgt gatggcagga aagatcgccc tggaagacgc ctacaatgcc
       61 cgcgaccgcg acgggatcag gctggtggac gagctggagc ggatcggcta cctcggcccg
      121 gagccctgcg tgccgcgccc cttccgggcc tatctggagc tgcacatcga gcaggggccg
      181 ttcctggagg agtcgaggct ctcggtgggc gtggtggagg gcatcgtcgc catttcctgg
      241 tcgcggctgg cgatccacgg cgtccaggac cacgcgggac cgacgcccat gcgcatccgc
      301 cacgacgccc tcgtcgccgc cgccgccgtc gtcaccggcg tgcggtcgat cgccggccag
      361 ctcggcggtg acgcggtgac gaccgtcggc acgctcgacg tggtccccaa catcgtcaac
      421 gcgattcccg gccgcgtccg gctcagcatc gacgtgcgcg atccccggac agaagccctc
      481 gaccgggcgc gcgccatgat cgaggccgtg gtgcgcgaag cgtgcgagcg cgagggcgtg
      541 cgctacgagc tggagcacta ctggcgcgtg cccgccacgc cgttcgaccg cgccgtggtg
      601 gacgccgtgg agcgggcggc gaaggccagc ggcgcgcggt atcggcgaat gacctccggc
      661 gccgggcacg acgcccagta catggccgcc ctcgggccga ccggcatggt cttcgtgccg
      721 tcgcgcgacg gtcgcagcca ctgcgaggag gagttcaccc cgatggacga catcgagcac
      781 ggcgcgaaca ccttgctgct gaccgcgctc gacctggccg gccgcgcctg agcggcccga
      841 ggagaggcag atgaggatcg atgcgtacgt gaagtccgtg ctcaccgtga tcgccgtggc
      901 cctcgtcgcc atcgcgctca acccgtggct cgccgtgctc accccgcgcg gcgtgcaggc
      961 tcagacggtc acgccgaagt acgagatcac gctcctcaag gcgtggggca agatcgtggg
     1021 cttctcgaac cagaacctcc tgatggaggc gcccgacggc accttgcgtc tggttgacct
     1081 cgagggaaag gcccccgagt atccgaaagt gaagtcgtac atgcgctggc agtagacggc
     1141 ctgggaggcg gccgccaccg gagtctcgtg gattccgcca gttgaccctg gcaccctgag
     1201 acacgatctc aatggctggg cccgccgctc cgcagggacg accggcatcg gaaagcgcga
     1261 cgacgagcgc gactcacgcg tgcttggcga acgaggtcag gcggatggtg ctcgctactc
     1321 ggggggcggc aacgcgtcgc cggtcacgta cgggtggtac gcgccggcac cggcccggcc
     1381 gcggacgcgc aggcgagcga cgacgggcag gtggtcggag gccacgcggg caagccggct
     1441 ccggtggacg tcgaaggtct cgccctcgag ctccacgtcc cagtagatcc ggtcgagctt
     1501 gaagagcggg aacatcgcag ggtgcgtccg gcgcatccgt cgcatcggcg aggagaactc
     1561 gcgcttgagg ccgcggctca ccggcccggg atgccactcg ttgaaatcgc ccatgagcac
     1621 gcgcggaccc gccagcgccg cggagtcgcg gatgaacccg gccagcgtct ccgcctgcgc
     1681 gcgccgctcg cgcaggccga gaccgaagtg gcagttgaac acgtgcaggg tgcggccgtt
     1741 ggcgccgagg tccacccgca ggcacgcccg cggctcgcgg ccatcgcggc tcaggtcgca
     1801 cgaggacgag ccgagcacgg gcaaccgcgt cagcaccgcg ttgccgtagg tgccctgccc
     1861 gtgcgcgcgc gtgacgcaca tgaccagcgc catgcccagg cgggaggcca ggtacgaggc
     1921 ctgatcggca tggacgtggc cgtcctcgcg cacgacctcc tggagaccga tcacgtcggg
     1981 cgtcatctcg ccgatcacgt cggcgacccg ctcgagatcg cggcgacggt cgagtcccac
     2041 gccccggtgg atgttgtagc tggcgacgac caggtgcacg ccgcggtcag tcacgctggt
     2101 acgcggcgaa gtgcgggtag agctgggtcg gatgcgtcag cggctccggc agcccgatcc
     2161 ccggccggca cagcacgaag cactccatct cctcgggcga ggggccggca tgcgcgccga
     2221 tctcggccac gtacgagatg ttgccgtcgg gcgagtcgtg gccgtagatc acgaggtcgc
     2281 cggcgcagcg catcgccatg aggtcgcgga cgcccgctgc gacgagcggc cagtcggccc
     2341 ggccggcgaa gggaccgacg gcctcaccgt cgagggcgta gcgcttgccg cgccagaggc
     2401 agagggggcc gggacgcgag cgcgccagca cgaagccgac gccgcggctg cgtccgatct
     2461 cgtcggcgag cccggggaag cgctcgtcga tgtcgtcgat cgtcagtgga tcggggtggt
     2521 cgaggaagta cacgaacgcg ttcggcccgg ctccgatgac acgcacgccg tcgcgctcgc
     2581 gggactccgg cagctcgccc atcagcttcg ggaagtcgcg ctcgaggtag ttggcgaagc
     2641 gctggaacat ccctggcgcg cggcgcgagc ggaacgcctc gatccccgcc gcgagccgac
     2701 gcccgcgggg ggcggcgggg cccacctcca ccatccccgc cggccggaag aactcctcga
     2761 agaacacccg ctcgatcggc tggccgccgc tgaccttccg gtacgaacgg cacgtcgtct
     2821 gcccgtgatc ggacagcacg tacatctcgt aacggtggcc cggcacccgg ccgatgacgc
     2881 gccagagctg ccggagcgag ctgtcgacgt ggcggagcga gcggagcgcg cggcgatggc
     2941 gcgggccgaa cgcgtgcgcg aacacgtcgt agtcgaggta gttgacgtag acggccggcg
     3001 cgcccgcgta gaggtcgcgc gagacggcca gggtgaagag ctcgcgcaac cagaccgaca
     3061 gcccgatctt gagggcgagc cacttccagc cgcgccgggt ctcgccgacc gggtcggcga
     3121 cgaagcggag cagggcgcgg gtcagctcga cgccggtgag cgccaggcac ttgaccacga
     3181 cccatgtgag cacgacaaag gccgatatca cgcgcagcag cccgtgaccg gtcggccgct
     3241 tgagcgtggc gaagttgaag aggttgttca ccgcaccgcc ggtgaacacg caggcatagg
     3301 cgctgccccc ctcgaggatg ccgcgccggc cggccgcctg cgtggcctcc acgtgggcgg
     3361 cgtcgcctcc acgcggaaag tagatgtccg agcgccggcg cttgtcgtgg tagtggaagc
     3421 cggggatgtc ggggcgcacg ccgtagaaga cgcccatctg gaaggccggc gtcgaggtcg
     3481 gcaggccgac gctcatcggc gccatccgcc accgctcgcg gcggagcagc cgctcgagga
     3541 acggcatgcg gccctcggcg agcgcccgct ccaggacgtc ccgcgcgagg ccgtcgatct
     3601 ggacgacgag caaccgcttc ctcgcaccgc gctcgggcgc cggcccgagg cgcagtcgcc
     3661 ggatggcgcg atcgatgcgt cgctgcagcg ccaggaccag ctcgtcgaga gccatgggat
     3721 ccattgtaat atcacgggga accgatgccc gccgcggtgc atcccctgct cgaggacgtc
     3781 cggcgcttcg tcgacgcgga ggtgatcccc gcggcgtcgg cgctcgagca cgccgacgcc
     3841 tacccgcacg ccctggtcgc gcgcatgcgc gcgctcggcc tcttcggcgc gctggtgccg
     3901 cgcgcgtggg gtgggctcgg cctcgacgtg acgacgtacg cgcgggtgat cgcggagctg
     3961 tgccgtggct ggatgtcgct ggccggcgtg atcaacagcc acacgatggc cgcgctgatc
     4021 gtcctccacc acggcaccga ggcgcagcgc gcgcgcctgc tgccgcgctt cgcgagcggc
     4081 gccgcccgcg gcggcctctg cctgaccgag ccgcacgccg gctccgacgt gcaggcgatc
     4141 cgcaccgtcg cgcgacgccg gggcgaggac tacctcctga acggcaccaa gatgttcgtc
     4201 accaacgggc gcgagggcaa caccttcgcc ctcctcgccg tcaccgactc cgcggcggcc
     4261 ccccgccacc gcggcatgtc gtgcttcatc gtcgagaagg gcgacgagcc cgaggccagg
     4321 ggcctgacgg tcgtgaagtc gatctccaag ctcggctaca agggcgtgga caccgccgag
     4381 ctgctcttcg aggacttcgc cgtccccggc gccagcctcg tcggcggcgt cgagggccgc
     4441 gggttcaagc acgtgatgag cgggctcgag acgggccgca tcaacatcgc cgcgcgcgcc
     4501 gtcggcgtcg gccaggcggc gtgggacgcc gcgcgcgccg acggccggcg tcgcgagccg
     4561 ccgccgcccg tgctggccga catggccgcc cggctcgaga ccgcgcgcct gatcaccgtc
     4621 tgggcggcgg gcatgaagga ccgggccgag cgctgcgacc tggaggcggg catggccaag
     4681 ctccacgcct cggagacggc gcagcggctg gccgaggacg ccctgcgggt cggcggcgag
     4741 gccgccgcgc tcgcgtcgtc ggtcgtcgag cggctctacc gcgacacgcc gctgatgatc
     4801 atcggcgagg gcacgaacga gatccagcgc acgctgatcg cgcgccagct cctcgagcgt
     4861 cacggcgagc gcctcggcgc cctcaccggg cgcgacgccg agtccgagga gcgccgccag
     4921 atcgtgctcg ccgtccgcca gttcgtggag aaggcggtcg tccccgtcgc gtgggagggc
     4981 gagcgcgcag cgcgctaccc cgaagacctg gtgcgcgacc tggcccatct cggcgccctc
     5041 ggcgccctgg cgccgcccgc gctcggcggg ctcggtctcg acctgcgcac gtatgcgatg
     5101 ctcgtcgagg agctggcgcg cggctggacc gcggtcgccg caatcgtggc cggccacgcg
     5161 acggtgacgt gggcgatcac gcagcacgga gttcgccacc tgcgggagca ctggctgccg
     5221 ccgatgacgc gcggcgagat cctgggggcc gtggcgctcg acggtgacgt gaccgcgcgg
     5281 gcagaaggcg gcacggtcat cctccacgga cgcacggccc tcgtggacgc ggcgccgcgg
     5341 gccggtgtcc acctcgtgcg cgcgcgtctc ggcggcggcc gtagcgtgct ggccgtcgtc
     5401 ccgcgcggca cgccgggcgc gagcgtgggt gcggtccagc ccaccctcgg cgctcgcgga
     5461 ctcgaggtcg cgccggtgga cttcgacggc gcccgcgtct ccgctagcgc ggtgctcgaa
     5521 gctggggccc tccgggccca cccgaccggc gacgcgtcgg tgtcggccct cggcctcgcg
     5581 cgcctcggtc tcgcggcgac ggccgttggg ctcgcgcagg cggcgttcga ggcggccctc
     5641 cgctactcgc agcagcgctc cgccttcggg caggcgatct gccaccacca ggcgatccag
     5701 ctcaagctcg ccgacatggc caccgccatc accgccgcgc ggctgctg
//
LOCUS       DATIAZ010000076         4116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1596426, whole genome shotgun sequence.
ACCESSION   DATIAZ010000076 DATIAZ010000000
VERSION     DATIAZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596426"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VLF19_02555"
     CDS             <1..522
                     /locus_tag="VLF19_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSE92160.1"
                     /translation="WRRTIGRPASEAIASLIDRALAAGEAHELSRRKHAHYVRLSRGN
                     LPAVRGACAYLAALSEAGVPCAVATSARRVDATSLLGGLGLLPHFRAVVTAEDVQRGK
                     PDPEVYRLAARGVGVAPDRCLVFEDSLVGVHAARAAGMRVVGLTTAHTEAELRAAGAE
                     RAIADFEGLAWPP"
     gene            510..1757
                     /locus_tag="VLF19_02560"
     CDS             510..1757
                     /locus_tag="VLF19_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92161.1"
                     /translation="MATVGAPGYRNSVLVVALLLAVGCASHSRPDQVLRKPVGADLAG
                     LVDSVPAQHLLADLLQRRTPEAHLAILAASLRSTDFVPDHDVRPRPDRARLRDLAREV
                     SLDFAALAFARELSADERSRAVQTAFERFLQDGAAGSEEALKKPGAFPYTVLFAPSWL
                     YRSHPETGSDLARPRGLVDRLGLDGRLIETDESGSVEANATVIAAAVRAAGRQGARLI
                     VVSASKSGAEAALALSRLLAPEDAAGVAAWVNVVGALGGTPIADTFSRPPASWFARAV
                     CWLAGWRWEGVTSMATGPSRKRLEGATMLDSIAVVNVVAVPVSGSVGHRVYGGYEFLR
                     PHGPNDGVVVLADTVWPGGANLVTLGPDHLYTPLWDDAHALALFRAVDFAVRLHGARM
                     AHGVATGAPEPRQRREAAGLDPP"
     gene            1835..2083
                     /locus_tag="VLF19_02565"
     CDS             1835..2083
                     /locus_tag="VLF19_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HSE92162.1"
                     /translation="MRTTLTLDDDVAALLKRVLARRKTSFRSLVNEALRQGLRGMTAP
                     RGRPEPYRTPAKSLGRCLLGNVDDVAEVLAVAERESFR"
     gene            2080..2508
                     /locus_tag="VLF19_02570"
     CDS             2080..2508
                     /locus_tag="VLF19_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSE92163.1"
                     /translation="MILVDANLLVYAATSGLPQHDRVRSWLDDRLNGAAPVGLPWASL
                     LAFIRLVSNPRVFEHPRPVADAWEQVTSWLGCAPVWVPQPTERHAEILGELLRRSVPR
                     SNLVPDAHLAALATEHGLVLCSTDGDFGRFPGLRWENPLG"
     gene            2582..3907
                     /locus_tag="VLF19_02575"
     CDS             2582..3907
                     /locus_tag="VLF19_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92164.1"
                     /translation="MKSLRVVLALALVGAIASATLPSPSVDAQGPTIKIGLLYDHTGP
                     FSAAGSLNSWRGAKMIIDYVNERGGVMGKYKIVQVDGDSQSKTEAAINEAERLLNVEK
                     VDILAGIYSSAHAVPLAEKVDRQKRFLWITTAIADAVVKDRNLQYTFRPQPNGSLFGE
                     ASVQYVAANAQGKLKKTAKDVRAAIIYEDGPYGAGVAASNESEARKQGMQVVLKEGYS
                     AQAPDLSSLVTKLRASRPDVIFHTGYNPDIALFLRQAKEQGLRTRIYVGHGAGHSQLA
                     KLKEALGNDVEGFHTVDPPAAQLVDSKRLKPGIAELTQEMLKRYKAFEPAIPTGAVAP
                     HVSMGFNNMWILLNDVLPRAIQKHGGFGAEALAKAARETDIAEGGTMQGYGVKFNPPG
                     HAMAGQNARALAAVFQVVDGEFKHVYPPVVASMASPPVLLPASSPFAAR"
BASE COUNT          563 a         1532 c         1443 g          578 t
ORIGIN      
        1 tggcggcgca ccatcggccg gccggcgagc gaggccatcg cctcgctgat cgaccgcgcg
       61 ctggcggccg gcgaggcgca cgagctctcc cgccgcaagc acgcgcacta cgtgcggctc
      121 tcgcgcggca acctgccggc cgtccgcggt gcctgcgcgt acctggccgc gctgagcgag
      181 gccggcgtcc cctgcgcggt ggcgaccagc gcccgccgcg tggacgcgac gagcctgctc
      241 ggcggcctcg ggctgctgcc acacttccgc gccgtcgtca ccgccgagga cgtgcagcgg
      301 ggcaagcccg accccgaggt ctaccggctg gccgcccgcg gggtcggcgt ggcccccgac
      361 cggtgcctcg tcttcgagga ctccctggtc ggcgtgcacg cggcgcgcgc cgccggcatg
      421 cgcgtggtcg gcctcaccac ggcccacacc gaggcggagc tgcgcgccgc cggcgccgag
      481 cgcgccatcg ccgacttcga ggggctcgcg tggccaccgt aggcgcgccc ggctaccgca
      541 attccgtgct cgtcgtggcg ctgctgctgg ccgtcggctg cgccagccac agccgaccgg
      601 atcaggtcct ccgcaaaccg gtaggcgcgg acctcgcggg cctggtggat tccgtgccgg
      661 cgcagcacct gctcgccgat ctcctccagc gccgaacgcc cgaggcgcac ctcgccatcc
      721 tggccgcgag cctccgcagc acggacttcg tccccgatca tgacgtgcgt ccgcgccccg
      781 accgggcgcg gctgcgcgac ctggcgcggg aggtctcgct ggactttgcg gccctcgcct
      841 tcgcccgcga gctgagcgcc gacgagcgga gccgcgcggt ccagaccgcc ttcgaacgct
      901 tcctgcagga cggcgcggcc ggttccgagg aagccctgaa gaagcccggg gccttcccgt
      961 acacggtgct gttcgcgccg tcgtggctct accggagcca tcccgaaacg gggtccgatc
     1021 tcgcgcgtcc gcgcgggctc gtcgatcggc tggggctcga cggccggctg atcgagaccg
     1081 acgagagcgg gtcggtcgag gccaacgcca cggtcatcgc ggcggcggtg cgcgcggcgg
     1141 gccgtcaggg ggcgaggctg atcgttgtca gcgccagcaa gtcgggagcc gaggccgcgc
     1201 tggccctctc gcgcctgctc gcgcccgagg acgccgccgg cgtggccgcc tgggtcaacg
     1261 tcgtgggcgc gctggggggc acgccgatcg ccgacacctt ttcgcggccg cccgcgtcgt
     1321 ggttcgcccg tgcggtctgc tggctcgcgg gatggcgctg ggagggggtg acttcgatgg
     1381 cgaccggccc gagccgcaag cgcctggagg gcgcgacgat gctcgactcg atcgcggtcg
     1441 tcaacgtggt ggcggtgccg gtgtcgggca gcgttggcca tcgggtctac ggaggctacg
     1501 agttcctgcg cccccacgga cccaacgacg gcgtcgtggt cctggccgac acggtctggc
     1561 cggggggagc caacctcgtc acgctcgggc ccgaccacct ctacactccc ctctgggacg
     1621 acgcgcacgc cctggcgctg ttccgcgccg tcgacttcgc ggtgcggctg cacggcgcga
     1681 ggatggccca cggcgtcgcg accggtgcgc cggagccgcg ccagcgccgg gaggctgctg
     1741 gcctcgaccc gccctgaggc ggactgcgcc ggacgtgtga tgtctgaaac atcacacggt
     1801 tgacatcggc acatcacgcg gatagggtcg gagcatgcgc accacgctca ccctcgatga
     1861 cgacgtcgcg gcgctcctca agcgcgtcct ggcccggcgc aagacgtcgt tccggtcgct
     1921 cgtcaacgag gcgctgcgtc aggggctgcg gggcatgacc gccccacggg gccgacccga
     1981 gccgtaccgg acaccggcca aatccctcgg ccgctgtctc ctcggcaacg tcgacgacgt
     2041 cgccgaggtc ctcgcggtcg ccgagcgcga atcgttccgg tgatcctcgt cgatgcgaac
     2101 ctgctcgtct acgccgcgac ctcggggctc ccgcagcacg atcgggtccg ctcgtggctg
     2161 gacgaccgcc tgaacggcgc cgcgccggtg ggcctgccct gggcgagcct cctggccttc
     2221 atccggctcg tgtcgaaccc acgggtgttc gagcatccgc ggcccgtcgc cgatgcctgg
     2281 gagcaggtca cgtcgtggct cgggtgcgcg ccggtctggg tgccgcaacc gaccgagcgg
     2341 cacgcggaga tcctcggcga gctgctccgg cgctccgtgc cgcgctcgaa tctcgtcccc
     2401 gacgcccacc tggcggcgct ggcgacggag cacgggctcg tcctctgctc caccgacgga
     2461 gacttcggcc gcttcccggg cctgcgctgg gagaacccgc tgggatagga cgccggcggg
     2521 tggacggtgc cgggggcgcg tgctaacgta cgcccacttt cagccccagg aggtattccg
     2581 gatgaaatcg ctgcgcgtcg tgctggcact ggcgctcgtc ggcgccatcg cctctgccac
     2641 cctgccgagc ccgtccgtgg acgcgcaggg accgacgatc aagatcgggc tcctctacga
     2701 ccacaccggg cccttctcgg ccgccgggtc gctcaactcc tggcgcggcg ccaagatgat
     2761 catcgactac gtcaacgagc gcggcggcgt gatgggcaag tacaagattg tccaggtcga
     2821 cggcgacagc cagtcgaaga ccgaagcggc gatcaacgag gccgagcgcc tgctcaacgt
     2881 cgagaaggtc gacatcctcg ccggcatcta ctcgagcgcc cacgcggtgc cgctggccga
     2941 gaaggtggac cgccagaaga ggttcctctg gatcaccacg gccatcgccg acgccgtcgt
     3001 gaaggaccgc aacctccagt acaccttccg cccgcagccc aacggctcgc tgttcggcga
     3061 ggcctcggtg cagtacgtgg ccgccaacgc ccagggcaag ctcaagaaga ccgccaagga
     3121 cgtgcgtgcg gcgatcatct acgaggacgg gccctacggc gccggcgtcg ccgcgtcgaa
     3181 cgagtccgag gccaggaagc agggcatgca ggtcgtgctg aaggagggct actccgccca
     3241 ggcgccggac ctctcctcgc tcgtgacgaa gctccgcgcc tcgcggcccg acgtgatctt
     3301 ccacacgggt tacaacccgg acatcgcgct gttcctgcgc caggccaagg agcaggggct
     3361 ccgcacgcga atctacgtgg gccacggcgc cggccacagc cagctcgcga agctgaagga
     3421 ggcgctcggc aacgacgtgg aaggcttcca cacggtggat ccgcccgccg cccagctcgt
     3481 cgattccaag cggctcaagc ccggcatcgc cgagctgacc caggagatgc tcaagcgcta
     3541 caaggccttc gagcccgcca tccccacggg ggccgtcgcc ccgcacgtct ccatgggctt
     3601 caacaacatg tggatcctcc tcaacgacgt cctgccgcgc gcgatccaga agcacggcgg
     3661 gttcggcgcc gaggcgctgg ccaaggccgc gcgcgagacg gacatcgccg agggtggcac
     3721 gatgcagggc tacggcgtca agttcaaccc gcccggccac gccatggccg gccagaacgc
     3781 gcgcgcgctc gccgccgtgt tccaggtggt ggacggcgag ttcaagcacg tgtacccgcc
     3841 ggtggtggcg agcatggctt cgccgcccgt gctgctcccg gcctcctcgc cgttcgccgc
     3901 gcgttagcgg ggggcttcgc cccccttcta atgtctctcc ccaggcccga ctgcgccggc
     3961 acagccggcg gatcgaccgg atcggagggg tcgagacgtc ccctccgccc gactccgtcc
     4021 atcgattcgg tgcgcagtga tccgcggcct gggccgggcc gatgctgacc gtttccgggc
     4081 tgacgaagcg gttcggggga ttcactgcgc tcaacg
//
LOCUS       DATIAZ010000077         6056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_675947, whole genome shotgun sequence.
ACCESSION   DATIAZ010000077 DATIAZ010000000
VERSION     DATIAZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675947"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VLF19_02580"
     CDS             <1..322
                     /locus_tag="VLF19_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSE92165.1"
                     /translation="MAKAKYYLLTSDFIDGREAERIGLVSLCVPRAELLPRAFDVAGK
                     LAQGSQQAIRWTKRSLNNWLRMAGPIFDQSIALEMLTFMGEDVREGLRAIREKRPPRF
                     PSAR"
     gene            384..1520
                     /gene="pdhA"
                     /locus_tag="VLF19_02585"
     CDS             384..1520
                     /gene="pdhA"
                     /locus_tag="VLF19_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HSE92166.1"
                     /translation="MPRKILEPRFSVEHLSILDSDGNLDAALEPDLLPDQLKAIYRAM
                     LLGRRLDERMVRLQRQGRIGTFAPIKGQEASQIGSVWTLRPTDWMVPSFRETAAMLRR
                     GWPIEKLLLFFAGYLEGGRPAPEQRDLPVTIPVATQLPHAVGLAYAAQYRGDDVVVMA
                     YFGDGATSEGDFHEALNFAGVWHVPVVFVCQNNQWAISVPLKKQTHSRTLAQKALAYG
                     LPGIQVDGNDVLAVYAASREAVDRARAGDGPTLIECVTYRLGMHTTADDPTKYRTEEE
                     VRAWERKDPLTRFAAYLGKRNLLEDGLEAAVDAEIAEAVRRFEATDPADPLAIFDHVY
                     AELPPHLQAQRDELAKRLRDGTAAPPPAPPDPALPMRGQRQSRR"
     gene            1517..2497
                     /locus_tag="VLF19_02590"
     CDS             1517..2497
                     /locus_tag="VLF19_02590"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSE92167.1"
                     /translation="MSKLTMVKALNLALGQEMERDPDVLIIGEDVGVDGGVFRVTEDL
                     QRKFGSRRVIDSPLAEAAIVGSSVGMALYGLKPVCEIQFSGFAFQCFHQIENHAARYR
                     TRSQGRFHCQMVIRMPYGGGVRALEHHTESEEQFYAHIPGLKMVVPSGPRNARALLVS
                     AIRDPDPVVFFEAKALYHAAKEDVPDESETLPLGRAQVGREGRDLTLIAWGAMVRVAR
                     EAAETLEQEDGVGCELIDLLTVSPLDRETLRTSVQKTGRAVIVHEAPRSFGPGAEIAA
                     SIMEGAFLALEAPIRRVTAYDVALAGFARERATVPDTARVVAAARETLAF"
     gene            2505..3446
                     /locus_tag="VLF19_02595"
     CDS             2505..3446
                     /locus_tag="VLF19_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSE92168.1"
                     /translation="MPPAGWLALAVVLAVAGPSWASSAKPLTVVSFNVFHGGASSGLW
                     GDDHRLERRLAMAVEGLRALDPDVVALQEASSGWGRGNVAARLAAGLGLRHVWAPATS
                     RVFVPPFGQLLTWMMNFSEGPAILSRYPVAASEVHELPRCSARRFDPRVALVVDLDTP
                     WGRLPVFSTHTSRDDCQVRRVGELALARRGALPALVMGDFNSGEASDAIATLMNGAGF
                     VDVFRVANPGEPGATVWQRVDAPAPTVFRRVDYVFIVPGLEVTARVRASRVVLNTPVR
                     EPNGITLWPSDHYGVLAELELIVGPDMAPSPRALGPR"
     gene            complement(3461..4396)
                     /locus_tag="VLF19_02600"
     CDS             complement(3461..4396)
                     /locus_tag="VLF19_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE92169.1"
                     /translation="MLGRGLAWVALVVDDVEAAARGMERDLELPRFDCPAGRGARRVP
                     VLPVGAAALALFAPGDPFVGGERRTGVHHVAVAVEDLGAAARTARFGVSLPAPEPGLA
                     GAGRVALAPEATAGVRVWLTEPLVTKVPDGNGRVERIDHVGVASADNEAAIDVFTRRL
                     GFPLESTQTDLEIQMTVESFTSDRYGVVHHAREPRAVGGLRVAFVTVGDCELEFLQEF
                     DPRTGAQVIPGEPGTTRQDQGAIGRYIGARGAGLHHLALKVPDIDGTLDSLDRAGRRL
                     IDRAGRPGSRRARIGFVHPAGFHGVLLHLVQREAR"
     gene            4426..6033
                     /locus_tag="VLF19_02605"
     CDS             4426..6033
                     /locus_tag="VLF19_02605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSE92170.1"
                     /translation="MARPFLLPDLGEGLTEAEIVKVLVREGDVVPEDAPLLEVETDKA
                     QVEIPSPLGGRVETIHVQPGETVRVGQVLVTFADAGAAAALPAAGPEAGARPMPAGTP
                     VATPDPTRRLAPGTRTTATDGRARPVEPAGAPSGGGPVPATPSTRRLARELGVDLRSV
                     RGSGSGGRVTDDDVRAAVGGGAALAQGRSTATPTTPAASLAERGADEERRPAKPLATV
                     GLEPPPLPRFEQWGPVERQPLSHLRRTIAERMTLSAALIPHVSHFDRADITDLDALIR
                     RNLEPARERGVTLTLTGFLLKAAALALGEHPTFNASLDPAAGELIVKRYVHVGVAVAT
                     ERGLIVPVIRDVDRKPVLEVARELAALAQRVRKGQASLDDLRGGTFTITNLGALGGTG
                     AIPIINYPEVAILGVARAREEAVVRDGQIVPRLLLPLSLTFDHRVADGAAGARFASAI
                     VRRLEHPDQLLLDLEVRARSAGATAMLAPPGPPGVAGALDAQPTTKTAQVGALGAATV
                     RNPDAFPRGSRDLGRVEAREATEAGPW"
BASE COUNT          805 a         2240 c         2194 g          817 t
ORIGIN      
        1 catggccaag gcgaagtact acctcttgac ctccgacttc atcgacggcc gggaggccga
       61 gcgcatcggc ctcgtgagcc tctgcgtgcc gcgcgcggag ctgttgccgc gggccttcga
      121 cgtggccggg aagctcgcgc aggggagcca gcaggcgatc cgctggacca agcgctcgct
      181 caacaactgg ctgcgcatgg ccgggccgat cttcgaccag tcgatcgcgc tggagatgct
      241 caccttcatg ggtgaggacg tccgcgaggg cctccgcgcc atccgcgaga agcgccctcc
      301 gcgcttcccg tcggcgcgct gacggaagcc ggagagcatc cgacccgtcg ggttttccgg
      361 tacactggtc tcgagccggg cccatgccca gaaagatcct cgagcctcgg ttctccgtcg
      421 agcacctgtc catcctcgac agcgacggca atctggacgc cgcgctcgag cccgacctgc
      481 tgcccgacca gctgaaggcc atctaccgcg cgatgctgct cggccgccgg ctcgacgagc
      541 gcatggtgcg gttgcagcgg cagggccgca tcggcacctt cgcgccgatc aagggccagg
      601 aggcctcgca gatcgggagc gtctggacgc tgcgcccgac cgactggatg gtcccctcgt
      661 tccgcgagac ggccgccatg ctccggcgcg gctggcccat cgagaagctg ctgctcttct
      721 tcgcgggcta cctcgagggc ggccggccgg cgcccgagca gcgggacttg ccggtgacca
      781 ttccggtggc gacccagctg ccgcacgccg tcggtctggc ctacgccgcc cagtaccgcg
      841 gcgacgatgt ggtggtgatg gcgtacttcg gcgacggcgc gacctccgag ggagacttcc
      901 acgaggcgct caacttcgcc ggcgtctggc acgtgcccgt ggtgttcgtc tgccagaaca
      961 accagtgggc catctccgtg ccgctcaaga agcagacgca ctcgcggacg ctcgcgcaga
     1021 aggccctcgc ctacggcctc cccggcatcc aggtggacgg caacgacgtg ctcgcggtgt
     1081 acgcggcgag ccgggaagcg gtcgaccggg cgcgcgccgg cgacggcccc acgctgatcg
     1141 agtgcgtgac gtaccggctg ggtatgcaca ccaccgccga cgacccgacc aagtatcgca
     1201 ccgaggagga agtgcgcgcg tgggagcgca aggacccgct cacacgcttc gccgcgtacc
     1261 tgggcaagag gaacctcctc gaggacgggc tcgaggcagc ggtcgacgcg gagatcgccg
     1321 aggccgtgcg ccgcttcgag gcgaccgacc cggccgaccc cctcgcgatc ttcgaccacg
     1381 tctacgccga gctgccgccg cacctgcagg cccagcgaga cgagctggcc aagcggctcc
     1441 gcgacggcac cgcggcgccg ccgcccgccc ctcccgatcc ggcgctgccg atgcgcggcc
     1501 agcggcagag ccgccgatga gcaagctcac gatggtcaag gcgctcaacc tggcgctcgg
     1561 ccaggagatg gagcgggacc cggacgtgct gatcatcggc gaggacgtcg gcgtcgacgg
     1621 cggcgtgttc cgcgtgaccg aggatctcca gcgcaagttc ggcagccgcc gcgtcatcga
     1681 cagtcccctc gccgaggcgg ccatcgtcgg cagctcggtc ggcatggcgc tctatggcct
     1741 caagcccgtc tgcgagatcc agttctccgg cttcgccttc cagtgcttcc accagatcga
     1801 gaatcacgcg gcgcgctacc ggacgcgctc gcaggggcgc ttccactgcc agatggtgat
     1861 ccgcatgccg tacggcggcg gcgtcagggc gctcgagcac cacacggagt ccgaggagca
     1921 gttctacgcc cacatccccg ggctcaagat ggtggtgccc tcggggccgc gcaacgcccg
     1981 cgcgctgctc gtgagcgcca tccgcgatcc cgatccggtc gtgttcttcg aggccaaggc
     2041 gctctaccac gccgcgaagg aggacgtgcc cgacgagagc gagacgctgc cgctcgggcg
     2101 cgcgcaggtc gggcgcgagg gacgcgacct cacgctgatc gcgtggggcg cgatggtgcg
     2161 cgtggcccgc gaggcggctg agacgctcga gcaggaggac ggcgtcgggt gcgagctgat
     2221 cgacctgctc acggtctcgc cgctcgaccg cgaaacgctg aggacgtcgg tgcagaagac
     2281 gggacgcgcg gtgatcgtcc acgaggcgcc ccggagcttc ggaccgggtg ccgagatcgc
     2341 ggcctcgatc atggagggtg cgttcctcgc gctcgaggcg ccgatccggc gcgtcaccgc
     2401 gtacgacgtc gccctcgccg gcttcgcgcg cgagcgggcc acggtgccgg acaccgcccg
     2461 cgtcgtcgcg gcggcccgcg agacgctggc cttctaactc cccggtgccg ccggccgggt
     2521 ggctcgccct cgctgtcgtg ctcgccgtcg ccgggccgtc gtgggcctcc tccgcgaagc
     2581 cgctgaccgt cgtcagcttc aacgtgtttc acggcggcgc gtcgtccggc ctgtgggggg
     2641 acgaccaccg tctcgagcgc cggctcgcga tggcagtcga ggggctacgc gccctggatc
     2701 ccgacgtggt cgcgctgcag gaggcctcgt ccggctgggg acgtggcaac gtggcggcac
     2761 ggctcgccgc gggtctcggg ctgcggcacg tgtgggcgcc ggccacgagc cgcgtcttcg
     2821 tcccgccgtt cggtcagctg ctgacgtgga tgatgaactt ctccgagggc cccgcgatcc
     2881 tcagccggta ccccgtcgcc gcctcggagg tgcacgagtt gccccgctgt agcgccagac
     2941 gcttcgaccc ccgcgtcgcg ctggtcgtgg atctcgacac gccgtggggc cggctcccgg
     3001 tcttctcgac gcatacgtcg cgtgacgact gccaggtccg tcgcgtcggc gagctggcac
     3061 tggctcggcg gggggctctg cccgcgctcg tcatgggcga cttcaactcc ggcgaggcat
     3121 cggacgcgat cgcgaccctc atgaacggcg ccggtttcgt ggacgtcttc cgcgtggcga
     3181 acccgggaga gccgggcgcc acggtgtggc agcgtgtcga cgcgcccgca cccacggtct
     3241 tccggcgcgt ggactacgtc ttcatcgtgc ccggactcga ggtgacggcc cgcgtgcgcg
     3301 cgagccgggt cgtgctcaac acgccggtgc gggagccgaa cggcatcacg ctgtggccgt
     3361 ccgaccacta cggcgtactg gcggagctgg agctgatcgt cggccccgac atggccccca
     3421 gcccccgagc gctcggtcct cgatgagcac gacggggtcg tcagcgggcc tcgcgctgga
     3481 cgaggtggag gaggacgccg tggaaccccg ccggatggac gaagccgatg cgcgcgcgac
     3541 gcgatccggg gcgcccggca cggtcgatca ggcgccgccc ggcgcggtcg agcgagtcga
     3601 gggtgccgtc gatgtcgggc accttcagcg ccaggtgatg caggccggcg ccgcgggcgc
     3661 cgatgtagcg gccgatggcg ccctggtcct ggcgtgtggt cccgggctcg ccggggatca
     3721 cctgcgcgcc ggtgcgcggg tcgaactcct gcaggaactc gagctcgcag tcgcccacgg
     3781 tcacgaacgc cacgcgcagg ccgccgaccg cccggggctc gcgggcatgg tgcacgacgc
     3841 cgtaccggtc ggaggtgaac gactcgacgg tcatctggat ctcgaggtcg gtctgcgtgg
     3901 attccagggg gaagccgagc cggcgcgtga agacgtcgat cgccgcctcg ttgtcggcgc
     3961 tcgcgacccc gacgtggtcg atgcgctcga cgcggccgtt gccgtctggc accttcgtca
     4021 cgagcggctc ggtgagccag acgcggacac cggccgtcgc ctcgggcgcc agcgccacgc
     4081 ggccggcgcc ggcgagcccc ggctcgggcg cgggcaggct cacgccgaac ctcgcggtgc
     4141 gcgcggccgc gccgaggtcc tcgacggcga ccgcgacatg gtgcacgccg gtccgccgct
     4201 ccccgccgac gaacggatcg ccgggcgcga acagcgccag cgcggcggct cccaccggca
     4261 gcaccggcac ccggcgcgcg ccgcggcccg ccggacagtc gaagcgcggc agctcgaggt
     4321 cgcgctccat cccgcgtgcc gcagcctcca cgtcgtccac gaccagcgcg acccaggcca
     4381 gcccgcgtcc gagcatggcg cgattgtagc ggtagaatag cctcgatggc gcggcccttc
     4441 cttctgcccg acctgggcga aggcctcacc gaagccgaga tcgtgaaagt cctcgtgcgc
     4501 gagggcgacg tcgtgccgga ggacgccccg cttctcgagg tcgagaccga caaggcacag
     4561 gtcgagattc cgtcgccgct cggcggccgc gtcgagacga tccacgtcca gcctggcgag
     4621 acggtacggg tagggcaggt gctcgtcacc ttcgccgacg ccggagccgc ggccgcgctt
     4681 ccggccgccg ggcccgaggc cggggcgcgt ccgatgccgg ccgggacgcc ggtggcgacc
     4741 ccggatccga cgcgccggct cgcgcccggg acacggacga ccgcgaccga cggccgcgcc
     4801 cgcccggtcg agcccgcggg agcgccgtcg ggcggcggcc ccgtgccggc gacgccgtcg
     4861 acgcgtcggc tggcgcgcga gctcggcgtc gatctccgct cggtgcgcgg tagcggttcc
     4921 ggcggccgcg tgacggacga cgacgtgcgc gcggcggtgg gtggcggtgc cgcgctggcg
     4981 cagggtcggt cgaccgcgac gcccacgact ccggcggcgt cgctcgcgga gcgcggcgcc
     5041 gacgaggagc gccgcccggc gaagccgctc gcgacggtgg ggctcgagcc gccgccgctg
     5101 ccacgcttcg agcagtgggg gcccgtcgag cgccagcccc tctcgcacct gcgccggacc
     5161 atcgccgagc gcatgacgct ctccgccgcg ctgatcccgc acgtcagcca cttcgatcgc
     5221 gcggacatca ccgatctcga cgcgctcatc cgacgcaacc tggagccggc gcgcgagcgc
     5281 ggcgtgacac tgacgttgac gggcttcctg ctcaaggccg cggcgctggc gctgggtgag
     5341 cacccgacct tcaacgcgag cctcgatccc gccgccggcg agctgatcgt caagcgctac
     5401 gtccacgtcg gcgtcgccgt cgccaccgag cgcggcctca tcgtgccggt gatccgcgac
     5461 gtggaccgga agcccgtcct cgaggtggcg cgcgagctgg cggcgctggc gcagcgcgtg
     5521 cgcaaggggc aagcctccct ggacgacctg cgcggcggca ccttcacgat caccaatctc
     5581 ggcgcgctcg gcggcacggg cgcgatcccg atcatcaatt accccgaggt cgcgatcctc
     5641 ggcgtggcgc gcgcgcggga agaggcggtc gtgcgcgacg gccagatcgt tccccgcctg
     5701 ttgctgccgc tctcgctgac cttcgaccac cgtgtggccg acggtgccgc tggcgcgcgc
     5761 ttcgcctcgg ccatcgtgcg ccgcctcgag catccggacc agctcctgtt ggatctcgag
     5821 gtgcgcgcgc gcagcgccgg cgctaccgcc atgctcgccc cccccggccc accgggcgtc
     5881 gccggtgcgc tcgacgcgca acccacgacg aagaccgcgc aggtgggcgc cctcggcgcc
     5941 gctactgtcc ggaaccccga cgcgttcccg cggggctcgc gagatctcgg ccgggtcgag
     6001 gcgcgcgagg ccacggaggc cggcccttgg tgatgggaga cctcgtgcgc gaggtg
//
LOCUS       DATIAZ010000078         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_544877, whole genome shotgun sequence.
ACCESSION   DATIAZ010000078 DATIAZ010000000
VERSION     DATIAZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544877"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VLF19_02610"
     CDS             complement(<1..319)
                     /locus_tag="VLF19_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92171.1"
                     /translation="MATRVPAADRTRERLEALINGRLATAPERSDLMLLAARLILEEA
                     LEGEVRDRLGRERYERAEGEPTGYRNGYRTGRMKAAEGLSALPTAPSSCRPKIAKGET
                     LPIL"
     gene            complement(412..2027)
                     /locus_tag="VLF19_02615"
                     /pseudo
     CDS             complement(412..2027)
                     /locus_tag="VLF19_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973759.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2082..>2308)
                     /gene="trkD"
                     /locus_tag="VLF19_02620"
     CDS             complement(2082..>2308)
                     /gene="trkD"
                     /locus_tag="VLF19_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Responsible for the low-affinity transport of
                     potassium into the cell; involved in potassium ion uptake
                     under hyper-osmotic stress at a low pH; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HSE92172.1"
                     /translation="AFAYLVITWRRGRAVLWDKLYGPQPAVAAFIAGLAPGLIRVRGT
                     AVYLTGNPEVVPTALAHNIGFSRSLRAAGS"
BASE COUNT          327 a          819 c          832 g          330 t
ORIGIN      
        1 gtaggatcgg cagcgtctcg cctttcgcga tcttcggccg acaacttgac ggtgccgtcg
       61 gcagagccga caggccctcg gccgccttca tccgacccgt ccggtacccg ttgcggtagc
      121 ccgtcggctc accctcggca cgctcgtagc gctcgcgtcc cagccgatcg cgcacctcgc
      181 cttccaaagc ctcttccagg atcagccgcg ccgccagcag catcaggtcc gagcgctcgg
      241 gcgccgttgc cagccggccg ttgatcagag cctccagacg ctcgcgtgtc cggtcggcgg
      301 cgggtactct cgttgccatg ggatgctccc tggggatccc ggcgccaacc ggaagcccgc
      361 tttcctgtgc tcactcaggc agggtgcatc ccaacctcgt ttccagcaaa cttacgcctt
      421 caccgcctgg aggacgcggg cgcgcaaagc gggaagccgg cacgcccgta catggtgcgc
      481 ttgatcatct tgagacggtt gatctgcccc tcgacggggc tcgtggtcca cggcaggtcg
      541 agggcggcct gcacggcctc gatgtccttg cgcagctcgg cgacaaagcc ggcgagcgcg
      601 gtggcctccg ccgcggccag cacgtcgtgg agcggctggt cgctcttgcg ctgcaggacg
      661 agggcgagct gggtgcccgc ggtgaccgtc gcagccaacg taggcgcctc ctccagcagc
      721 cgggcggcaa aggcccggtc ggcatcggag agcgtcttgg gctcggccat cagcagccgc
      781 gtcaggcgac gtcccgaggg cggccgccac acgtctttcc cgcccgtggg cgccctggtc
      841 gcgtcaggcc cggtctgacg tcgccgggcg gcccaggtcc tcacgatggt gtacccgccc
      901 gaaaagcctg tcccgagcag ctcgcgccac agccgggcgg cgttgcggca gccttgggcc
      961 cagcgccgtt ccaggtactc gtgatgcgga tcgagcatgc tgccgcgccg cggctggcgc
     1021 cagagcggca cgccgccggc ctgcagccag cgccgcagtg tcttgtggtc caccccgagc
     1081 agccgggaga tccggctgac ggacatgccc gccgcgtgca gccgggccgc ttccatgtag
     1141 cgggcgtgcc ggcgctcatg ggctgtcggc ttgcaccggt tggcagcatt cggctcggcg
     1201 gtggtgcggg tcgatgcgga tgcggcatcg gcagggttct cggccatctt gtcggcggcc
     1261 cgccggacgg cggcatgatg gcgctcgacc gccgcctgca cggcgccgcc gagattgcgc
     1321 aggagatgcc atcggtcggc gacttgcacg gcctcgggcg cgccttggcg gatgccgtcg
     1381 gcataggcgc cggcacgatc ccgggccacg atccgcacgc cggggttccg cttcagccat
     1441 gccgccaaag acccggcttg acggtcgggg agcaggtcca ccacccggtt gcgctcgagg
     1501 tcgaccaagg ccgtgccgta gcggtgtccg cggcgccagg cccagtcatc gacgcccagc
     1561 acgcgcgggc tcggctgtgg tccggcatcg ctgccggtta tgcaggccag ccgcagcagg
     1621 gtgtccgggc tgaccggcat ggccagccgt gccgcgagcc gcgccccggc ctccccgccg
     1681 agggcgagac caagacagcg ctgcaggctg cccagccgtt cggtccggcg tgccgccagc
     1741 ggcgcgacct cggccaggcg ctcggcgaag gtctgtcgtg agcatgaggc attgaggcag
     1801 cggaaccgcc gcgcctggat gtgcagcgtc accggacggc cctgccaggg caagtcgccg
     1861 agccgacggt cgtagcggct gtgaacccgt cgcgacggca ccgcgcagtc gggacaggcg
     1921 gccgagttcc ggcgcgggcg ggccaggatg gtgatgcggt tggaggcagg caggagctgt
     1981 tggaccagca gacccgccgg gatcaggggc aaaaggcgct tggacaagga tggctttcga
     2041 aggaggacaa gtccctccta tgtgctcacc caggcccgcc atcacgaccc ggcagcacgc
     2101 aaactgcggg agaacccgat gttgtgcgcc agcgccgtcg gcacgacctc ggggttgccg
     2161 gtcaggtaca ccgccgtgcc gcggacccgg atcagcccgg gagcgagccc ggcgatgaag
     2221 gccgcgacgg cgggttgggg gccgtacagc ttgtcccaga gcacagcgcg gccgcggcgc
     2281 caggtgatca ccagataggc aaacgcgg
//
LOCUS       DATIAZ010000079         6210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1859961, whole genome shotgun sequence.
ACCESSION   DATIAZ010000079 DATIAZ010000000
VERSION     DATIAZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859961"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VLF19_02625"
     CDS             <1..326
                     /locus_tag="VLF19_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92173.1"
                     /translation="VTAAGAQDLDLEAPAPLREPRVPVPEPGPGPSVPYDPAFIRPFT
                     IDRETPDSTGRMGLSGWTAPSTHVGLAGVGEREIGGWLSLGFTWTWGGPPLLTPAPPG
                     ASPPP"
     gene            464..1153
                     /locus_tag="VLF19_02630"
     CDS             464..1153
                     /locus_tag="VLF19_02630"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSE92174.1"
                     /translation="MVGGAHHAAAAGATDADFAALRTQMVEDQLRAPGRSITDRRVLE
                     AFAAVPRHEFVPEASRADAYADRPLPIGHGQTISQPFIVAFMTERLDLKPTDRVLEVG
                     TGSGYQAAILSVLVSEVYTIEIVEPLARRAAADLKRLGYENVKVRIGDGYKGWPEAAA
                     FDAIIVTCAPDHVPRPLVDQLKDGGRMVIPVGSRDDQKLYLLEKRGQELRRRAVLPVR
                     FVPMTGSGVKK"
     gene            complement(1196..1318)
                     /locus_tag="VLF19_02635"
     CDS             complement(1196..1318)
                     /locus_tag="VLF19_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92175.1"
                     /translation="MRVQGTAQLAFGAAAVGVFFGFYPARKASLLNPIDALRHE"
     gene            complement(1612..2808)
                     /locus_tag="VLF19_02640"
     CDS             complement(1612..2808)
                     /locus_tag="VLF19_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSE92176.1"
                     /translation="MKVVIEALAQAPVARRRIELVERKGLGHPDTICDSLAEAVAVGL
                     NRMYLAEAGTIPHYNVDKALLVAGQCAKTFGRGEITRAMELILGDRATSEVGGRRLPV
                     EETVHAAVAAWLAAHLPEVRPGRDLLTRVALCPGSAELRNILAAASGSIVSNDTSGAA
                     GWAPLSPTEVTVLAVERFLNGEEFKRRFPDTGQDVKVFGLREDDRLDVTVAMPLRCRD
                     VASERAYFRRKEEILAALAERFRDAPVSLEFRLNNLDSPGAGADGVYLTLTGTSAEDA
                     DSGEVGRGNGVNGLIAFSRPAGGEAAAGKNPVAHAGKIYSVLSHRLASLIHARHPELP
                     EVYVHLAARIGEPVDRPWTGVQIVLAEGMRQEDVDKTVRNVVEAELARLPEFRGELIR
                     GKHPVC"
     gene            complement(2805..3491)
                     /locus_tag="VLF19_02645"
     CDS             complement(2805..3491)
                     /locus_tag="VLF19_02645"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSE92177.1"
                     /translation="MTASAAETARFAKARDRMVEIQLAGQGIRDPAVLAAMRAVPRDR
                     FVPEHLAEEAYADRALPIGLGQTISQPYIVAAMLELVEPKPGDRALEIGTGSGYSAAV
                     LATIVAEVYSVECLADLAQSAARRLAALGYATVHVRLANGTLGWPEHAPYDVIVVTAG
                     GPRVPPPLLDQLAVGGRLVMPVGGLLGGQRLVRVRRRSETDYDREELEDVAFVPLVGA
                     EGWPSGGPPP"
     gene            complement(3498..3770)
                     /locus_tag="VLF19_02650"
     CDS             complement(3498..3770)
                     /locus_tag="VLF19_02650"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92178.1"
                     /translation="MNLHYRFPPASAYRLNRCLHALKSDDAFRDRYRADPDGAAASMG
                     LSPEETAVLRAGDRDALVARGAHPYLVFMAELRLTMARGTAEIEYF"
     gene            complement(3767..4552)
                     /locus_tag="VLF19_02655"
     CDS             complement(3767..4552)
                     /locus_tag="VLF19_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92179.1"
                     /translation="MLVGAFALAHTPQLLARPATEDRDLVLRVHAAFARIKRRLAALR
                     LDAIAMVGGDHVEAFFLNAVPALAVYAGRECAGTFGRYRYRFPIHEPLARAVVEQGIE
                     RGFDLLYTQDAPLDYAFFVPLHFSMPDPPVPIVPLHVNVYLPPQPTPRRCYDWGRALG
                     DILGHRPERIALVASGGMSHYPGTDRYASPDFEWDRQILERLREGRGRDLASLTGEEL
                     DKAGNVELRTWITVLGAVGDAPAAVYCYEPSWHHGNAVVEWRP"
     gene            complement(4552..5076)
                     /locus_tag="VLF19_02660"
     CDS             complement(4552..5076)
                     /locus_tag="VLF19_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4792..4794),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HSE92180.1"
                     /translation="MPDETYLDPTDSVAVPRRAAPRPSSLDGKVVTLLDISKAKGDHL
                     LDRIEALLRERARPRAIVRRKKPTFARPAPDDLRREISGATDVVIEALADUGSCTTCS
                     VHDGVFFEDAGIPTATVISSEFVRAARAQAEALGATDYRTVVVPHPIQPLTREEVQAL
                     ADKAFDEILRQLTA"
     gene            complement(5371..6042)
                     /locus_tag="VLF19_02665"
     CDS             complement(5371..6042)
                     /locus_tag="VLF19_02665"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSE92181.1"
                     /translation="MMRVVCFIALLLLVVVLPAAPVRGQDSRECRVVEDFARAKVGEF
                     PAGWEVRKEAGKATYSVQEEGGLRFLRAHSKGLGIQAAKQHAWDLSAYPVMAWSWRAR
                     EFPWRAHEKDGKNDSVLAVYMLVPHSRTVGPKAVKYVWSEHVAAGTRLESNNGLTQVR
                     VLRQGTEKRGEWVEERVNVRGDYAEYFQDHEISKPAGIAILTDSDDTRSSAQGDYANF
                     RICRG"
BASE COUNT          902 a         2193 c         2240 g          875 t
ORIGIN      
        1 gcgtgacggc ggccggcgcc caggacctgg acctcgaggc gcctgcgccg cttcgcgagc
       61 cccgcgttcc cgttcccgaa ccgggtcctg gcccgagcgt gccctacgat cctgcattca
      121 tccgtccgtt cacgatcgac cgcgagacgc ctgactccac cggacgcatg ggcctctcgg
      181 gctggacggc gccgagcacg cacgtcggac tcgccggcgt cggggaacgc gagatcggcg
      241 ggtggttgag cctcggtttc acgtggacat ggggcggacc gccgcttctc accccggcgc
      301 cgccaggagc cagcccgccc ccgtgacgcg gatgggtaca tcggcgatct ccgggccccc
      361 gccggggaag gccgagcgcc ggcacgtcct ttcgagccct gcgcaccgac gggggatcgt
      421 gcgcagaatc tcctggctgc tgtgctctct tcacctcgca tcgatggttg gcggggctca
      481 tcacgctgcc gcggccgggg ctacggacgc cgactttgcc gccctgcgca cacagatggt
      541 ggaagatcag ctcagagcgc cgggtcggag catcaccgac cggcgggtgc tcgaggcctt
      601 cgccgcggtg cctcgacacg aattcgtgcc cgaagcatcc cgcgccgacg cgtacgcgga
      661 ccgtcccctg cccatcgggc acggccagac gatctcccag ccgttcatcg tggcgttcat
      721 gaccgaacgg ctcgatctca agcccacgga ccgcgtgctc gaggtcggca ccggctccgg
      781 ctatcaggca gcgatcctct ccgtgctcgt gtcggaggtc tacacgatcg aaatcgtcga
      841 gccgctggcg cgccgggccg cggcggatct gaagcgactg ggctacgaga acgtgaaggt
      901 caggatcggc gacggctaca aggggtggcc ggaagcagcg gccttcgacg cgatcatcgt
      961 gacctgcgcc ccggaccacg taccgcggcc attggtggat cagctgaaag acggaggaag
     1021 gatggtcatc ccggtgggat cgagggacga ccagaagctg tatctgctgg aaaagcgcgg
     1081 gcaagagctc cggcggcgtg ccgtgttgcc ggtgcgcttc gttcccatga ctggcagcgg
     1141 agtaaagaaa tagcttcgcg ccgaggcggc ggcgtcggcg cgaccggggc cgccgtcact
     1201 catgacgcag ggcatcgatc gggttgagga gcgaggcctt gcgcgccgga tagaagccga
     1261 agaagacgcc gacggcggcg gcgccgaagg cgagctgcgc agtgccctgc accctcatgg
     1321 ccgtcccggg atccgagcgc cgctcgccgg cacctggcgg gcgtcgaacg gtgtggagac
     1381 gggctcgtcc taggtcagct caccgtctcg gaagcgcagg atgcgccgcg cgtaccgggc
     1441 gatgtcctcc tcggggcgac gagcacgatg gtgatgccgc tccggttcag cgcctggagg
     1501 atcgccgcga tctcgacgct ggtccgggtc tccaggttcc cggtgggctc gtcggggcgg
     1561 tactgtccta tcgacgggcg gcgcggcgag gcaggcgtcg cgctcggtcg ctcagcacac
     1621 cgggtgcttg ccgcggatca gctcgccgcg gaactccgga agccgcgcga gctctgcttc
     1681 gacgacgttc cggacggtct tgtcgacgtc ctcctgccgc atgccctccg cgagcacgat
     1741 ctgcacgccc gtccacgggc ggtccaccgg ctcgccgatg cgcgccgcca ggtggacgta
     1801 gacctcgggc agctcgggat ggcgggcgtg gatgagcgac gcgaggcgat ggctcagcac
     1861 gctgtagatc ttgcccgcgt gcgccacggg gttcttcccc gcagccgcct cgccgccggc
     1921 cgggcgggag aaggcgatga ggccgttgac cccgttgccg cggccgacct cgccggagtc
     1981 ggcatcctcg gccgaggtgc cggtgagagt gaggtagact ccgtcggcgc cggcgcccgg
     2041 cgaatcgaga ttgttgagcc tgaactcgag cgagaccggg gcgtcgcgaa agcgctccgc
     2101 gagcgccgcc agaatttcct ccttgcggcg gaagtacgcg cgctccgacg cgacgtcgcg
     2161 gcacctgagc ggcatcgcca cggtcacgtc gagccggtcg tcctcccgga ggccgaagac
     2221 cttgacgtcc tggcccgtgt cgggaaagcg tcgcttgaat tcctcgccgt tcaggaagcg
     2281 ctcgacggcg agcacggtca cctccgtcgg gctcaggggc gcccagcccg cggcgcccga
     2341 ggtgtcgttg gacacgatcg agccggaggc ggcggcgagg atgttcctga gctcggcgga
     2401 gccggggcag agggccacgc gcgtcaagag atcccggcca gggcggacct cggggaggtg
     2461 cgccgccagc caggccgcga cggctgcatg cacggtctcc tcgaccggca gccttcgtcc
     2521 gccgacctcg gacgtggcgc ggtcgcccag gatcagctcc atggcccgcg tgatctcgcc
     2581 tcggccgaag gtcttggcgc actggcccgc gacgagcagc gccttgtcga cgttgtagtg
     2641 gggaatcgtg cccgcctcgg cgaggtacat gcggttgagc ccgacggcga cggcctcggc
     2701 gagcgaatcg catatggtgt ccggatgacc gagacccttg cgctcgacca gttcgatccg
     2761 gcggcgcgcc acgggcgcct gggcaagcgc ctcgatcacg accttcatgg gggcggcccc
     2821 ccggacggcc agccctccgc gccgaccagc gggacgaagg cgacgtcctc cagctcctcc
     2881 cggtcgtagt cggtttcgct cctccggcgc acccggacca ggcgctgccc gcccagcaga
     2941 cccccgaccg gcatcacgag tcgtccgccg acggcgagct gatccagcag gggcggcggc
     3001 accctgggac cgcccgcggt gacgacgatc acgtcgtagg gcgcgtgctc cggccaaccg
     3061 agggtgccgt tcgccaggcg cacgtggacg gtcgcgtagc cgagggcggc gagccggcgg
     3121 gctgcggact gcgccagatc ggcgagacac tcgaccgagt agacctcggc gacgatcgtc
     3181 gcgagcacgg ccgcagaata gccggagccg gtcccgatct cgagagcgcg atctccgggc
     3241 ttcggctcca ccagctcgag catggccgcg acgatgtacg gctgggagat cgtctggccc
     3301 aggccgatcg ggagcgcccg gtcagcgtac gcctcctcgg cgaggtgctc gggaacgaag
     3361 cgatcgcggg gcaccgcgcg catcgccgcg agaaccgccg ggtcgcggat cccctgcccg
     3421 gcgagctgga tctcgaccat gcggtcgcgc gccttcgcga accgggccgt ttcggccgcc
     3481 gaggcggtca tcgcccgtca gaaatactcg atctcggcgg tgccgcgcgc catggtgagc
     3541 cggagctcgg ccatgaagac caggtacgga tgcgcgccgc gcgcgaccag cgcgtcgcgg
     3601 tcaccggcac gcagcacggc ggtctcctcg gggctcagcc ccatgctcgc ggcggcgccg
     3661 tcggggtcgg cacgatagcg gtcgcggaag gcgtcgtcgg atttcagcgc gtgcaggcaa
     3721 cggttcagcc ggtaggcgct ggccggtgga aagcggtagt gcaggttcac gggcgccact
     3781 cgaccacggc gttgccatgg tgccaggacg gctcgtagca gtagacggcg gccggcgcgt
     3841 cgcccaccgc gccgagcacc gtgatccacg tgcgcagctc cacgttgccc gccttgtcca
     3901 gctcctcgcc ggtcagcgac gcgaggtccc gcccgcggcc ctcgcgcagg cgctcgagga
     3961 tctgccggtc ccactcgaag tccggcgacg cgtagcggtc ggtgcccgga tagtgggaca
     4021 ttccgcccga ggccacgagg gcgatgcgct ccggacggtg cccgaggatg tcgccgagcg
     4081 cacgacccca gtcgtagcag cggcgcggcg tgggctgggg cggcaggtag acgttcacgt
     4141 gcagcgggac gatgggcacc gggggatcgg gcatcgagaa gtggagcggg acgaagaacg
     4201 cgtagtcgag cggcgcgtcc tgcgtgtaga gcaggtcgaa gccgcgctcg attccctgct
     4261 cgaccacggc gcgcgcgagc ggctcgtgga tggggaagcg gtaccggtag cgaccgaacg
     4321 tgcccgcgca ctcccgcccg gcgtagacag ccagggcggg cacggcgttc aggaagaacg
     4381 cctcgacgtg gtcgccgccg accatggcga tcgcgtcgag ccgcagcgcg gcgagccggc
     4441 gcttgatgcg ggcgaaggcc gcgtgaacgc gcaggacgag gtcgcggtct tcggtggcgg
     4501 gccgcgcgag gagctggggc gtgtgggcca gagcgaaggc gccgacgagc atcaggccgt
     4561 cagctggcgc aggatctcgt cgaacgcctt gtcggcgagc gcctgcacct cctcgcgcgt
     4621 gagcggctgg atggggtgcg ggacgacgac ggtccggtag tcggtggcac cgagcgcctc
     4681 ggcctgcgcc cgcgccgccc gcacgaactc cgacgagatc accgtcgccg tggggatgcc
     4741 tgcgtcctcg aagaacacgc cgtcgtgcac actgcacgtc gtgcaggacc ctcagtcggc
     4801 gagcgcctcg atgacgacgt cggtggcgcc ggatatctcc cgccgcaggt cgtcgggcgc
     4861 cggccgcgcg aaggtcggct tcttcctgcg cacgatggcc ctgggcctgg cccgctcgcg
     4921 caacagcgcc tcgatgcggt cgagcaggtg atcacccttc gccttggaga tgtcgagcag
     4981 ggtgacgacc ttgccgtcga gggacgacgg ccgcggcgcc gcccgccggg ggacggccac
     5041 ggagtcggtg ggatccagat acgtctcgtc gggcatcgcg cgcctccttc accccatcgc
     5101 gaccctgcgg aacacgagcc ggcaccaccg gacgctcctc ggcggcggac gttcgccagg
     5161 tcgccggccg cgctcgggcg gcgctggacg cggggcaggc cgtcgggccg cggcgccccc
     5221 cagcgcctcg atcgggtcgt cgtcggccca cgggtcggcg acgggcgggc ttccgctcat
     5281 ggcaggaggc tatcacggct cgtggcgctg taccggcagg gcgccagccc ccgccgctcg
     5341 ccgccgagcg cgcgaggccc ctccgatgag ctagccgcgg cagatccgga agttggcgta
     5401 gtctccctgg gcgctggacc gggtgtcgtc ggagtccgtg aggatcgcga tccccgccgg
     5461 cttggagatc tcgtggtcct ggaagtactc tgcgtagtcc ccgcggacgt tgacgcgctc
     5521 ctcgacccat tcgccccgct tctcggtgcc ctgccgcagc acccgcacct gcgtgagccc
     5581 gttgttggac tcgagccgcg tgccagcggc cacgtgctcg ctccagacgt acttgacggc
     5641 cttgggaccg acggtccgcg agtggggcac gagcatgtag acggcgagca cgctgtcgtt
     5701 cttcccgtcc ttctcgtggg cgcgccacgg gaactcccgc gcccgccacg accacgccat
     5761 caccgggtag gcggagaggt cccacgcgtg ctgcttggcg gcctggatgc cgagtccctt
     5821 cgagtgtgcg cgcaggaagc ggagcccgcc ctcctcctgc acggagtacg tggccttgcc
     5881 cgcctccttg cgcacctccc agccggccgg aaactcaccg accttcgctc tcgcgaagtc
     5941 ctccaccacc cggcactccc gcgagtcctg tcctcgaacg ggagctgccg gcaaaacgac
     6001 aaccaagagc aagagcgcga tgaagcacac cacgcgcatc atctctcacc cccgaatggg
     6061 acgaaggcgc ctcggcagct acctgccgcg gcgccaccac cggtggacga gctggtccac
     6121 gacctcgccg agctgcgcga tcgtccggca ctcgcgaacg atgtcgcatg ccggcgcgta
     6181 gagcggcatc acgctgtcgc cgacacccca
//
LOCUS       DATIAZ010000080         4056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_412990, whole genome shotgun sequence.
ACCESSION   DATIAZ010000080 DATIAZ010000000
VERSION     DATIAZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412990"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VLF19_02670"
     CDS             <1..1082
                     /locus_tag="VLF19_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSE92182.1"
                     /translation="MERNVEMFAACDEAFDRGRLIAIYPEGATRAEAYLQRIKTGAAR
                     IALAYEAHAPGRLTVVPVGLSFAARKRFRGRVLVSFGEPVDVSSHLASYREEPAKALH
                     ALTTTIQGAMEREVVHVERIDIAAFGRAVETLYRGEIECELWDERWVSGRQAVPVQSS
                     GSIAAAVEHFCKQDPGRIEGLWQRILGYHVGLADYRLRDETVRTRLERTAERQRVARS
                     WQTIVGLPLFAYGAAVNFLPYYLPGWLAGRMSRRMTDYATIRLLVSVVAFPLFWALET
                     ALVGWAARLRWASVFFLSLPLGGLIAYRYLVGTERLRHQLRFGALLLTRAQEARRLLA
                     ERREIIEELERAKRDYVRGAQGPSV"
     gene            complement(1108..1530)
                     /locus_tag="VLF19_02675"
     CDS             complement(1108..1530)
                     /locus_tag="VLF19_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HSE92183.1"
                     /translation="MPAVVLEAANFVISFCVITFLFGMIFKLLPDVYVAWRDVWLGAA
                     LTSLLFTLGKVVIGLYLGLATISSAYGAAGSLLVILLWVYYSAQILLFGAELTKVWAR
                     RRGAGAVPTEAAVALTEEARAEQGMARAARSAGRRSSA"
     gene            complement(1494..2036)
                     /locus_tag="VLF19_02680"
     CDS             complement(1494..2036)
                     /locus_tag="VLF19_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HSE92184.1"
                     /translation="MVEAWRLLRDTVGDWYQNRAQRLGAALAFYTLFALAPGLVILIP
                     LTGVFLGPQAAQGHIVEQIESLIGRPGAEAIEATMVSAREEGAGVTVVAVITLVFGLW
                     WVFGELQDALNTIWGVTSRPGRGFLVILRERFWSFTLVVGIGFLLLVSLAVSAWRPRW
                     AGSSPTSCRCPQWYWKPPTS"
     gene            2104..2358
                     /locus_tag="VLF19_02685"
     CDS             2104..2358
                     /locus_tag="VLF19_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92185.1"
                     /translation="MKDRKAAPSSTTSKSKKASSRITAAKWEAAAAAGAAGRRAQRQA
                     IGQASVEKVARRQFQKTRGQAIQAHVAARGRRQQARRDSR"
     gene            complement(2368..3678)
                     /gene="pyk"
                     /locus_tag="VLF19_02690"
                     /pseudo
     CDS             complement(2368..3678)
                     /gene="pyk"
                     /locus_tag="VLF19_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
     gene            3926..>4056
                     /locus_tag="VLF19_02695"
     CDS             3926..>4056
                     /locus_tag="VLF19_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92186.1"
                     /translation="MIRRFVVLGASGDLASRHLLPALARLHEAGKLPAELSILGAAR"
BASE COUNT          646 a         1412 c         1432 g          566 t
ORIGIN      
        1 ggatggagcg caacgtggag atgttcgcgg cgtgcgacga ggccttcgac cgcgggcggc
       61 tcatcgccat ctacccggaa ggcgccactc gcgcggaggc gtacctacag cgcatcaaga
      121 cgggcgcggc gcggatcgcg ctcgcctacg aggcgcacgc tccgggacgc ctcacggtcg
      181 tgccggtcgg gctcagtttt gcggcccgca agaggttccg cgggcgcgtc ctcgtctcgt
      241 tcggcgagcc ggtcgacgtc tcgtcgcacc ttgccagcta ccgtgaggag ccggcgaaag
      301 cgctgcacgc gctcacgacg acgatccagg gggcgatgga gcgcgaggtg gttcacgtcg
      361 agcgaatcga catcgcggcg ttcgggcgcg ccgtggaaac gctctatcgc ggcgagatcg
      421 agtgtgagct gtgggatgag cgctgggtct cgggacggca ggccgtcccg gttcagtcgt
      481 ccggatcgat cgcggccgcg gtcgagcact tctgcaaaca ggaccccggg cgcatcgagg
      541 ggctctggca gcggatcctc ggttaccacg ttggccttgc cgactaccgg ctccgtgacg
      601 agacggtgcg gactcgcctc gagcggacgg cggagcggca gcgggttgca cggtcatggc
      661 agacgatcgt aggcctcccg ctcttcgcgt acggtgcggc cgtgaacttc cttccctact
      721 atctgcccgg gtggctcgcg gggcggatgt cgcgtcggat gacggactac gcgacgatcc
      781 gactcctcgt gagcgtcgtc gcgttcccgc tcttctgggc gcttgagacg gcgctcgtag
      841 gatgggcggc ccgtctccgc tgggcgtctg tcttcttcct ctcactcccg ctcggaggtc
      901 tcatcgcata tcggtatctg gtcggcacgg agcgcttgcg ccaccagctc aggttcggcg
      961 cgctcctcct cacacgcgct caggaagcaa ggcgcctcct cgctgagcgc cgtgagatca
     1021 tcgaagagct cgagcgcgcc aagcgcgact acgtgcgggg cgctcaagga ccgagcgttt
     1081 aggcacacgg atgagccgcg gtgttgatca cgcggaacta cggcgccccg cgcttctggc
     1141 cgcgcgcgcc atgccttgct cggctctggc ctcttcggtc agcgccaccg ccgcctcggt
     1201 cggcacggcg cctgcgcccc gccggcgcgc ccagaccttg gtgagctcgg ccccgaaaag
     1261 gaggatctgc gcggagtagt aaacccagag caggatgaca agcaacgagc cggcggcgcc
     1321 gtaggccgag ctgatcgtgg caaggcccaa atagagaccg atgacgacct tgcccagcgt
     1381 gaagagcagc gaagtcagag ccgcgcccag ccacacgtcg cgccacgcca cgtagacgtc
     1441 gggcagcagc ttgaagatca tgccgaagag gaacgtgatg acgcagaacg agatcacgaa
     1501 gttggcggct tccagtacca ctgcgggcac cggcaggagg tgggcgaaga acccgcccag
     1561 cgcggccgcc aggcgctgac cgccagcgag accagcagca ggaaaccgat cccgaccacg
     1621 agcgtgaagg accagaaccg ctcccggagg atcaccagga agccgcggcc cggccgggac
     1681 gtcacgcccc agatcgtgtt cagcgcgtct tgcagctcgc cgaagaccca ccacagcccg
     1741 aacacgagcg tgatcacggc gacgacggtc acgccggccc cctcctcgcg ggcgctcacc
     1801 atcgtggcct cgatggcctc cgcccccggc cggccgatca gggactcgat ctgctcgacg
     1861 atgtgtccct gcgcggcctg agggccgagg aagacgccgg tgagcgggat gaggatgacg
     1921 aggccgggcg cgagcgcgaa caacgtgtag aaagccagcg cggcgccgag ccgctgcgcc
     1981 cggttctggt accagtcgcc gacggtgtcc cggagcagtc gccacgcctc aaccataccc
     2041 gccgagcgta ccccggagcc gccaggatcg ctcgccggcc acgaaggacg tagaatcgtc
     2101 gccatgaaag acaggaaggc cgcgcccagc agcacgacat cgaagagcaa gaaggcttca
     2161 tcacgcatca ctgcggcgaa gtgggaggcg gcagccgcgg ccggcgccgc cgggcgacgc
     2221 gcgcagcggc aggcgatcgg gcaagcctcg gttgagaagg tcgccaggcg ccagttccag
     2281 aaaacacgcg ggcaggcgat ccaggcgcac gttgccgcac ggggccggcg ccagcaggcg
     2341 aggcgcgact cccgctgacg cagaccatca gagccgtccg gtccacagtc gatcgtgcga
     2401 cacgaacacg gcgcgcaggt cgggccagcc ccgctcccgg gccgcgcgcc gcgccacctc
     2461 gatcatctcc tcgggatcct gactcgatgc cggcaggagc agcggcagca cgctgcgcac
     2521 gagggccagt cgccgacagg tctccgggtc gccggtcaag gcgaggatcg gctgcgcggg
     2581 gcactgcttg gcggcgaggc gggccgtctc gccggtccgg gtcaccgtca cgaccacgtc
     2641 aatcccgcga tgggccgcga gctgacacgc cgcctggatc accgcctcct cgtcgctctg
     2701 cggcggctcc gccgacgcgc cggacggccg cgggccctgg agcacgcccc gctccgtctc
     2761 cagcgcgatg gcggccatga cctccacggc acgcaccggg tgccggccga ccgccgtctc
     2821 ctccgacagc atcaccgcgt cgctcccgtc gaggatggcg ttggccacgt cggcgacctc
     2881 ggcccgcgtc ggttgcggcg aggacaccat cgagaagagc atctgggtgg ccgtgatgac
     2941 tggcttggcc cgccggttcg cctcggcgat cagatgcttc tgcacgatgg gcacacgagc
     3001 gaacggtgtc tcgatgctga gatcgccgcg ggcgatcatg atcccgtcgg cggcgtcgag
     3061 gatctcgtcg aagtggtcca gcgccgcctg ggtctcgatc ttcgcgatca cgggcacgtg
     3121 gccgccgagg cgctcgatgt ggccccgggc ggctcgcacg tcctcggccg agcggacgaa
     3181 cgacagcccg aggtagtcga tcccctgctc caggccgacg cgcaggtcac gcaggtcctt
     3241 gtcagcgagg atgggcaggc cgggcagccc cgaggggacg ttgacgccct tgcggctgcc
     3301 gagacggccg ccgacgatca cctggcaatg gatctcctcg tcggtcacgc gctcgacacg
     3361 gaggaggatg acgccgtcgg cgaggagcag cgtgtcaccg acgccaacgg cagcggggag
     3421 ccccgcgtac gagacggaca cgcgatcacg ggagccctcg actgggcgcg tcgtgagggt
     3481 gaacccgtgc ccgggctcga gctcgacgct gccgtgcgtc agcgtgccga tgcggacctt
     3541 cgggcccgcg agatcttgca ggaccgccgc ggggatttcg agatcttggc tgagcgcgcg
     3601 gatgccggcg aggacggcgg cgtgctccgc aggctcaccg cgcgagaagt tgagccgggt
     3661 cacgttcgtc gcgcgctcga ggaaggcccg gagctacgcc cgggacgagg tggccggccc
     3721 gatcgtgcgg acgactttcg tccggcgctc catgctggcg atgttcttca ttcggctccc
     3781 gcgcgccgat ggtaacctcc ccgtccgttc gtcgtacact gggcgcgaag gtcgccggtg
     3841 gcgaccggaa caccacccgt gtgaaggacg accttaagct ccccgatccg gcctgcgtcg
     3901 gcatgggccg gcggacggtg accctgtgat ccggcggttc gtcgtcctcg gcgcgtcggg
     3961 ggacctcgcg tccaggcacc tcctgcctgc gctcgcccgc ctccacgagg ccggcaagct
     4021 cccggccgag ctgagcatcc tcggggcggc gcggga
//
LOCUS       DATIAZ010000081         5663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_281797, whole genome shotgun sequence.
ACCESSION   DATIAZ010000081 DATIAZ010000000
VERSION     DATIAZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281797"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VLF19_02700"
     CDS             complement(<1..756)
                     /locus_tag="VLF19_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92187.1"
                     /translation="MLPGFVRSHGLLRLPVALEGAAGALVGALVVLAWYGAGRLLVRF
                     VARLGPPEADRPHTLLAIARGCAYGAGLWSLAWLGLGLAGAYVAPVAAVALMAGLVMA
                     ALAVHGSRREHARAALTVSRWMGARGPEGAGDGVVTAADGLVDRGRGATSAALALVVF
                     SLAAGLLAALAPPTAKDTLQYHGALPRAFVAAEALVVVADNIASYFPLAVETQGVWAL
                     LLGRTVSARVGEAAFSVVAFAYFPLLVAAVYGWA"
     gene            complement(849..1334)
                     /gene="rplQ"
                     /locus_tag="VLF19_02705"
     CDS             complement(849..1334)
                     /gene="rplQ"
                     /locus_tag="VLF19_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSE92188.1"
                     /translation="MRHRAHRYRLGRVSEHRTALLRNLLVALFRHERITTTETKAKAV
                     RPLADRMVTLAKRESLHARRQVLSMVPDAAVVGRLFDTIAARFADRHGGYTRILRSGP
                     RPGDAAPMVFLELVDRAETPPDGKGKDEKKEKAPRGEGKREAKAAGRGKKKQRAAAAS
                     A"
     gene            complement(1331..2311)
                     /locus_tag="VLF19_02710"
     CDS             complement(1331..2311)
                     /locus_tag="VLF19_02710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSE92189.1"
                     /translation="MPRIPFQKPRKVEWEILSERYGRLVAEPFEKGYALTVGHSLRRT
                     LLAIVPGGAVSWMKIDGLKGPDAKIPGVSESTTDVVLNFKKLAVQVPSGEPRVIRLEV
                     AGPRTVTGADVPETDIEIVNPEVHLFTLDAGARVGIDIGVGVGRGYEAADRKIAPVPA
                     GAIGLDAAYSPITRVSYNVEMSRLGKITDYEKLILEIWTNGVVGPEDALERAANFLKG
                     HYSPLTIGVAEEETEVEGASGEEYLREALGKQLEELPLPARAINALKNAEIHLVVDLA
                     QKTEADLEQVKNLGEKSIDEIKTALAALGLSVGMRIDPNVLGALNKGGEK"
     gene            complement(2323..2946)
                     /gene="rpsD"
                     /locus_tag="VLF19_02715"
     CDS             complement(2323..2946)
                     /gene="rpsD"
                     /locus_tag="VLF19_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSE92190.1"
                     /translation="MARYRDAKCRLCRREGMKLFLKGARCFTDKCAIERRNYPPGQHG
                     LNRGKLTAFGVQLREKQKAKRIYGVLEQQFRRYFESAEREKGVTGENLLRFLELRLDN
                     VVHRLGFAASRREARQMVAHGHFQVRARKVSVPSYLVEVGDVVQLRPTSKLAARVDDN
                     LNAGRGGVPQWLEVDPEGRKGTVRSLPLREDIQIPVSEQLIVELYSK"
     gene            complement(2955..3359)
                     /gene="rpsK"
                     /locus_tag="VLF19_02720"
     CDS             complement(2955..3359)
                     /gene="rpsK"
                     /locus_tag="VLF19_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSE92191.1"
                     /translation="MADQAQAPRKKGGKKRERREVRTGVAHIQATFNNTIVTLTDKMG
                     NVVSWSSAGSAGFKGSRKSTPFAAQTAAEAAARKAMEYGLKSIEVFVKGPGAGREAAI
                     RSLQAVGLEITAIRDVTPIPHDGCRPPKRRRV"
     gene            complement(3363..3785)
                     /gene="rpsM"
                     /locus_tag="VLF19_02725"
     CDS             complement(3363..3785)
                     /gene="rpsM"
                     /locus_tag="VLF19_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSE92192.1"
                     /translation="MARVAGVDLPREKRGEVALTYIYGIGQPTAQRVLDQARVDPGKR
                     VKAWSEDEAARVREIIERELKVEGDLRRDVSMNVKRLMDVGAYRGIRHRRGLPVRGQR
                     THTNARTRKGPRKGVMVKKKPAAPAPAAAAAAPKKGGA"
     gene            complement(3855..3968)
                     /gene="rpmJ"
                     /locus_tag="VLF19_02730"
     CDS             complement(3855..3968)
                     /gene="rpmJ"
                     /locus_tag="VLF19_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HSE92193.1"
                     /translation="MKVRPSVKPRCEHCKIVKRAGVTRIICKNPRHKQRQG"
     gene            complement(4007..4225)
                     /gene="infA"
                     /locus_tag="VLF19_02735"
     CDS             complement(4007..4225)
                     /gene="infA"
                     /locus_tag="VLF19_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSE92194.1"
                     /translation="MAKEDAIEVEGTVVEPLPNAMFRVELENGHRVLAHVSGKMRMNF
                     IRILPGDRVKVELSPYDLTRGRITYRFK"
     gene            complement(4227..5081)
                     /gene="map"
                     /locus_tag="VLF19_02740"
     CDS             complement(4227..5081)
                     /gene="map"
                     /locus_tag="VLF19_02740"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSE92195.1"
                     /translation="MIVLKSPREIDLMRRGGLVLADLMDRLRAFVKPGMSTLEIDEEV
                     ETFITGRDARPAFKGYRGFPATVCVSINDEVVHGIPSAHRRLKEGDIVGLDLGCIIEG
                     YYADCAFTMALGEIGLEVQKLLDVTRESLEFAIQECRPGRRLGDVSHAVQRHVEANRL
                     AVVRAFVGHGIGRALHEEPQVPNFGEPGRGPVLKTGMVLAIEPMVTMGSCEVRILDDG
                     WTAITKDGSLAAHFEHTIAVTPEGPDVLTRRVAATSKGANVTAASEASPRTGAAGGAG
                     ARTPKTAH"
     gene            complement(5086..>5663)
                     /locus_tag="VLF19_02745"
     CDS             complement(5086..>5663)
                     /locus_tag="VLF19_02745"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSE92196.1"
                     /translation="RGVPQVATGDMLREAVGAGTSLGTTARSYMDRGALVPDDVIIGL
                     IAERLGRTDAAGGFILDGFPRTIPQAEALDHLLKDLGQSLDRVIYFDVPEAELLRRLT
                     GRRSCPVCQSTYHVVSAPPKREGSCDKCGASLVQRADDSEATVCQRLEVYARQTAPLL
                     DYYRGRGLLATVDGAGPIDTIQAAIRRAAEA"
BASE COUNT          754 a         2086 c         1932 g          891 t
ORIGIN      
        1 ggcccagccg tagaccgccg ccaccagcag cgggaaatac gcgaacgcca cgaccgagaa
       61 cgcggcctcg ccgacccgcg ccgagacggt acggcccagc agcagcgccc atacgccctg
      121 cgtctcgacg gcgagcggaa agtagctcgc gatgttgtcg gcgaccacga ccagcgcctc
      181 cgcggcgacg aaggccctcg gcagcgcccc gtgatactgc agcgtgtcct tcgcagtcgg
      241 cggcgccagc gccgcgagga gccctgcggc gagcgagaac acgacgaggg cgagcgccgc
      301 cgaggtcgcg ccgcgcccac gatccaccag accgtcggcg gccgtcacga ctccgtcgcc
      361 ggcgccctcc ggtccgcggg cgcccatcca gcgactgacg gtcagcgcgg cccgggcgtg
      421 ctcgcgacgc gagccgtgaa cggcgagcgc ggccatgacg agtcccgcca tcaacgcgac
      481 ggcggccacg ggcgcgacgt aggcgccggc gagcccgagg ccgagccacg cgagcgacca
      541 gagaccggcg ccgtacgcgc acccgcgcgc gatggccagc agggtgtgcg ggcgatcggc
      601 ctccggcggc cccagccgcg cgacgaaccg gacgaggagg cggccggcgc cgtaccaggc
      661 gagaacgacg agagcgccca cgagcgcacc ggcggcgccc tcgagagcga cgggcaggcg
      721 cagcaacccg tgggatcgga cgaagcccgg cagcacgccg agcgccgcgg cgaggtgcgc
      781 gaaatcggcg ccgtggacgg cgaggaacgc cgcggccagc gcgcaggcgg cggcgagcag
      841 gagcagggct acgccgacgc ggccgcggcc cgttgcttct tcttgcctcg gccggcggcc
      901 ttggcttcgc gcttgccctc gcccctgggc gccttctcct tcttctcgtc cttgcccttg
      961 ccgtcgggcg gggtctctgc gcggtccacc agctcgagga agaccatggg cgcggcgtcg
     1021 ccaggccggg gcccggatct cagaatgcgc gtgtagccgc cgtggcggtc ggcgaagcgc
     1081 gccgcgatgg tgtcgaacag ccggcccacg acggcggcgt ccgggaccat cgagaggacc
     1141 tggcgccgcg cgtggaggct ctcgcgcttg gcgagcgtga ccatgcggtc ggcgagcggc
     1201 cgcaccgcct tggccttcgt ctcggtcgtc gtgatccgct cgtgccggaa cagcgccacg
     1261 agcaggttcc ggagcagtgc cgtgcggtgc tcgctgaccc gcccgagccg gtacctgtgc
     1321 gcccggtgcc tcatttctca ccgcctttgt tcagcgcgcc cagcacgttg gggtcgatgc
     1381 gcatgccgac ggagagcccg agcgcggcca gtgcggtctt gatctcgtcg atggacttct
     1441 cgccgaggtt cttgacctgc tccaggtcgg cctcggtctt ctgggcgagg tcgacgacga
     1501 ggtggatctc ggcgttcttc agcgcgttga tcgcccgcgc cggcagcggc agctcctcga
     1561 gctgcttgcc gagggcctcg cgcaggtact cctcgcccga ggcgccctcg acctccgtct
     1621 cttcctcggc gacaccgatc gtcagcgggc tgtagtgtcc cttgaggaag ttcgcggccc
     1681 gctccagcgc gtcctccggc ccgaccacgc cgttggtcca gatctcgagg atgagcttct
     1741 cgtagtcggt gatcttgccc agccgcgaca tctcgacgtt gtacgacacg cgggtgatcg
     1801 gcgagtacgc ggcgtcgagg ccgatcgcgc cggccgggac cggcgcgatc ttgcggtcgg
     1861 cggcctcgta gccccggccg acgcccacgc cgatgtcgat gccgacacgc gcgcccgcgt
     1921 ccagcgtgaa gaggtgcacc tcggggttga cgatctcgat atcggtctcg gggacgtcgg
     1981 caccggtcac cgtccgcggc ccggcaacct cgagacggat cacccggggc tcgcccgagg
     2041 gcacctgcac cgcgagcttc ttgaagttca acaccacgtc ggtggtggac tcgctgacgc
     2101 cggggatctt cgcgtccggc cccttgaggc cgtcgatctt catccacgac acggcgcctc
     2161 cggggacgat cgcgagcagc gtccggcgga gcgaatggcc gacggtcagc gcgtagccct
     2221 tctcgaaggg ctcggcgacg agccgcccgt aacgctcgct caggatctcc cactcgacct
     2281 tgcgcggctt ctggaacgga atgcgcggca tgacggctcc tgctacttgc tgtacagctc
     2341 gacgatgagc tgttccgaca cgggaatctg gatgtcctcg cgcagcggaa ggctgcggac
     2401 ggtgcccttg cggccctcgg gatcgacctc gagccactgc gggacgccgc cgcgcccggc
     2461 gttgaggttg tcgtccacgc gggcggccaa cttcgacgtc gggcgcagct gcacgacgtc
     2521 accgacctcg acgaggtacg acggcaccga caccttgcgg gccctcacct ggaagtgccc
     2581 atgggccacc atctgccgcg cctcccgccg cgaggcggcg aagcccaggc ggtggacgac
     2641 gttgtccagg cgcagctcga ggaacctcag gaggttctcg ccggtgacgc ccttctcacg
     2701 ctcggccgac tcgaagtagc ggcggaactg ctgctcgaga acgccgtaga tgcgcttggc
     2761 cttctgcttc tcgcgcagct gcaccccgaa cgccgtgagc ttgccgcggt tcaggccgtg
     2821 ctggcccggt ggatagttgc ggcgctcgat cgcgcatttg tcggtgaagc agcgggcgcc
     2881 tttgaggaag agcttcatgc cctcgcggcg gcagagacgg catttggcat cgcggtatcg
     2941 cgccatggcc gcccctacac ccgccgccgc ttcggcggcc ggcagccgtc gtgcgggatc
     3001 ggcgtcacgt cacggatggc ggtgatctcg agccccaccg cctgcagcga ccggatcgcg
     3061 gcctcgcggc ccgctcccgg ccccttcacg aacacctcga tcgacttcaa gccgtactcc
     3121 atcgccttcc gggcggccgc ctcggccgcg gtctgggccg cgaacggcgt gctcttccga
     3181 gagcccttga acccggcgct gccggcgctc gaccacgaca ccacgttgcc catcttgtcg
     3241 gtgagcgtca cgatcgtgtt gttgaacgtc gcctggatgt gcgcgacgcc ggtgcgcacc
     3301 tcgcggcgct cgcgcttctt gccgcccttc ttgcgcgggg cctgggcctg gtcggccatg
     3361 gtctatgccc cccccttctt gggcgccgcg gccgccgcgg cgggcgccgg cgccgccggc
     3421 ttcttcttga ccatcacgcc cttgcggggg cctttccgcg tgcgggcgtt ggtgtgcgtg
     3481 cgctgcccgc gcacgggcag gcccctgcgg tggcggatcc cccggtaggc gcccacgtcc
     3541 atgagccgct tgacgttcat ggacacgtcg cgccgcaggt cgccctcaac cttcagctcg
     3601 cgctcgatga tctcgcgcac gcgcgccgcc tcgtcctcgg accaggcctt cacgcgcttg
     3661 ccggggtcga cgcgcgcctg gtccagcacc cgctgggccg tcggctggcc gataccgtag
     3721 atgtacgtga gcgcgacctc gccccgcttc tcccgcggaa gatccacccc tgccactcgt
     3781 gccatgtctc gtctccgcct tcctcgttcg cgcgccggct ctgccggcgc aatcctctcc
     3841 tggggaggtc gatgttatcc ctgccgttgc ttgtgccgcg ggttcttgca gatgatgcgc
     3901 gtcacgccgg cccgcttgac gatcttgcag tgctcgcaac gcggcttcac cgatggtctc
     3961 accttcatcg ctcgccctgt cctcttcccc gttcgcgccg gctctgctat ttgaacctgt
     4021 acgtgatgcg gccgcgcgtg aggtcgtacg gcgacagctc gaccttcacg cggtcgccgg
     4081 gcaggatccg gatgaagttc atccgcatct tcccgctcac gtgggccagg acacgatgac
     4141 cgttctccag ctccacccgg aacatcgcgt tcgggagcgg ctctaccacc gtgccctcga
     4201 cctcgatcgc gtcttctttt gccatctcag tgcgctgtct tcggtgttcg cgcgccggcg
     4261 ccgccggcgg cccctgtcct gggggaggcc tcggaggcgg cggtgacgtt cgcccccttc
     4321 gacgtggcgg ccacccgccg cgtgagcacg tccggtccct ccggggtcac ggcgatggtg
     4381 tgctcgaagt gggccgcgag gctgccgtcc ttggtgatgg ccgtccagcc gtcgtcgagg
     4441 atccgtacct cgcagctccc catcgtcacc atcggctcga tcgccagcac catgcccgtc
     4501 ttgagcacgg gcccgcggcc cggctcgccg aagttcggca cctgcggctc ctcgtggaga
     4561 gcccgcccga tgccgtggcc cacgaaggcg cgcacgacgg cgagacgatt cgcctcgacg
     4621 tggcgctgca ccgcgtgcga cacgtcgccg agccgacggc ccggccggca ctcctggatg
     4681 gcgaactcga ggctctcgcg cgtgacgtcg agcagcttct gcacctcgag gccgatctcg
     4741 ccgagcgcca tcgtgaacgc gcagtccgcg tagtacccct cgatgatgca gccgagatcg
     4801 agaccgacga tgtcaccctc cttgagccgg cggtgcgccg aggggatgcc gtgcacgacc
     4861 tcgtcgttga tggagacgca cacggtggcc gggaagcccc ggtagccctt gaacgccggc
     4921 cgggcgtccc ggcccgtgat gaacgtctcc acttcctcgt cgatctccag cgtggacatc
     4981 cccggcttga cgaatgcgcg cagacggtcc atgaggtcgg ccaggaccag tccgccccgg
     5041 cgcatcaggt cgatctcgcg cggtgacttg agcacgatca cgtcgtcacg cctccgccgc
     5101 gcgccggatc gccgcctgga tggtgtcgat cggacccgcg ccatcgaccg tcgccagcag
     5161 cccgcgtccg cggtagtagt ccaggagcgg ggccgtctgc cgggcgtata cctccagacg
     5221 ctggcagacc gtggcctcgc tgtcgtcggc acgctggacg aggctcgcgc cgcacttgtc
     5281 gcagctgccc tcgcgcttcg gcggtgccga gacgacgtga tacgtcgact ggcagacggg
     5341 gcaggaccgg cgtccggtca gccggcgcag caactcggcc tccggcacgt cgaagtagat
     5401 gacacgatcc agcgattgtc cgaggtcctt cagcaggtgg tcgagcgcct cggcctgcgg
     5461 gatcgtccgg gggaagccgt ctaggatgaa gccgcccgcg gcgtcggtcc gaccgagccg
     5521 ctcggcgatc aggccgatga tgacgtcgtc ggggaccagc gcgccgcgat ccatgtacga
     5581 ccgggcggtc gtgcccagcg aggtgccggc gccgacggcc tcgcgcagca tgtcccccgt
     5641 ggccacctgc gggacccccc gcg
//
LOCUS       DATIAZ010000082        13525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_939564, whole genome shotgun sequence.
ACCESSION   DATIAZ010000082 DATIAZ010000000
VERSION     DATIAZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13525
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939564"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1639
                     /locus_tag="VLF19_02750"
     CDS             8..1639
                     /locus_tag="VLF19_02750"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSE92197.1"
                     /translation="MKPGETRFIFVTGGVVSSLGKGLAAASIGCLLEARGFRVTLQKM
                     DPYINVDAGTMSPYQHGEVFVTDDGGETDLDLGHYERFTSARVTRQHNVTTGKVYFSV
                     IQKERRGDYLGRTVQVIPHITDEIKAAIRRVGGDVDVVIVEVGGTVGDIESLPFLEAI
                     RQLKKDVGKDNVIYIHLTLVPFIQAAQELKTKATQHSVKELRAIGIQPDVLLCRTDRY
                     LPPGIKSKIALFCDVEEEAVITAKDVDTVYEVPQVFHREGLDAIIVKLLGLPDRPSDL
                     SRWDEVVKRVKSPRHETRIAVVGKYIELRDSYKSLVEALTHGGIANDARVDLGWVDAE
                     HIERDGAAAHFADVHGILVPGGFGDRGIEGKIAAVRYAREAGVPYFGICLGMQCAVIE
                     FARHVCGLDRANSTEFDAATPNPVIDLLPEQRAIAAKGGTMRLGLYPIVLAEGSMAAR
                     LYGQSIIHERHRHRYEVNNDYLQRLEKNGLRVSGEWAEKGIVEMIEIPEHPYFVAGQF
                     HPEFRSRPWDPHPLFAGFIRAALGHARARRRASAP"
     gene            1636..2529
                     /gene="kdsA"
                     /locus_tag="VLF19_02755"
     CDS             1636..2529
                     /gene="kdsA"
                     /locus_tag="VLF19_02755"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HSE92198.1"
                     /translation="MSLSSAVRIGPVVVGAGHPLALIGGPCAIENEKHALMTAERLAA
                     IAAAHRVPFVYKSSYDKANRSSIDGYRGPGLVEGLRILRRVKEVVGVPVLSDVHQVEE
                     VEPAAEVLDVLQIPAFLCRQTDLVVAAARSGRPVNVKKGQFVAPDDMRNVIEKVRAAG
                     NDQLLLTERGTSFGYHNLVVDMRGLVQMRDLGVPVVFDATHSVQLPGAGGSRSGGERR
                     YVPALARAAVAVGIDALFMEMHEDPDRTLADGRPLSDGPNMLRMDDLPRLLDELLAIR
                     AAVLHTGRGAGTEASGDGTRR"
     gene            2540..3535
                     /locus_tag="VLF19_02760"
     CDS             2540..3535
                     /locus_tag="VLF19_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HSE92199.1"
                     /translation="MSDVRRRAARVLRMEAEAILALVPRLDERFDRAAELLYRCTGRV
                     VVAGMGKSGHVGRKIAATLASTGTPAYFLHPAEGMHGDLGMLAPGDVVLALSNSGETD
                     EMLAMLPTCKRLGVPIVLLTGNPKSTLARQCDVVLDVSVAEEACPMNLAPTSSTTAAL
                     AVGDALAMALLDLRGLRPEHFAALHPRGTLGWRSLIRVGDLMHAGPEMPVVSADTPMK
                     DVIVEMTDKRLGMTTVVDDQGRLMGVITDGDLRRAHLRAEPIANLTAAALATRRDFHR
                     DGDPRTKTIQAEDLAAKALEIMETHTITSLVIVDPAQRPVGIIHLHDVLRAKIDV"
     gene            3609..4163
                     /gene="lptC"
                     /locus_tag="VLF19_02765"
     CDS             3609..4163
                     /gene="lptC"
                     /locus_tag="VLF19_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HSE92200.1"
                     /translation="MDRLARRIFVIVALFVLVVTGTLVVRSRAMRTEAVGPTPSAADL
                     SIREVELQEETADGARWQLVADQAQVFDAEGRTALRNVRVRVQDRERTWTITGEEGDY
                     FKTAKDFELRGSVVIVSDDGLRLETTVLRWKGDGRRLWTDAPVRITRDGTVIEGTALE
                     MKMAEEATTVGGRVRAVFQPGQRS"
     gene            4175..4741
                     /gene="lptA"
                     /locus_tag="VLF19_02770"
     CDS             4175..4741
                     /gene="lptA"
                     /locus_tag="VLF19_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HSE92201.1"
                     /translation="MAGLALTAALLAPSGVAGQASRPAPKSQGPAPLRPKSPEARAQP
                     VTVHADKMDRYGKESLIIFTGQVVARQEGSVQTADRMEVYLDEKGDRILRTVSTGNVR
                     IVTGDCRIGTSHRAEYDDLEQRMVLIGDARVWQDDNVVTGDTITLFLSQDRSVVQGGK
                     QERVTAVFYPKPQDGPGTPRPTARQSCE"
     gene            4746..5468
                     /gene="lptB"
                     /locus_tag="VLF19_02775"
     CDS             4746..5468
                     /gene="lptB"
                     /locus_tag="VLF19_02775"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HSE92202.1"
                     /translation="MEGLVADGLTKWFKHRKVVDRVSLDIQRGEVVGLLGPNGAGKTT
                     SFYMMVGLLPADGGRIFLEGREITRLPMYQRCRLGVGYLPQESSVFRKLTVEENLLAI
                     LETLDLSAAERRERAQELLAELDLTALAPYPAYTLSGGERRRLEITRALVTSPQYLLL
                     DEPFTGIDPIAIGDIQEIVGRLRERGIGILITDHNVRETLAITDRAYILYDGKVLVSG
                     TASEIANNPTAREIYLGDRFSL"
     gene            5493..6959
                     /gene="rpoN"
                     /locus_tag="VLF19_02780"
     CDS             5493..6959
                     /gene="rpoN"
                     /locus_tag="VLF19_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HSE92203.1"
                     /translation="METRLSLRQSQRVVMTPLLQQAIQLLQLSTLELQEVVQKELLEN
                     PLLEEVPTDNADAPEAAATAPDAPAPAPPAAEPAPLEPPPVDKERGTDDLPFDLTAVM
                     FDDHEERSLVAQEERDDLPFENMVRSQSSLADHLDEQLRMVTEDTLVRRIGTEIIGNL
                     DEDGYLRAEVAEIAQRCQTTAEEVEKVLVLVQGFDPPGVAARSVQECLLIQLKSDPNP
                     DPVSVEIVEQHFDDLSRRRYPDIARALKLPLDRIMESVEEIMGLEPKPGRRFGGSDSR
                     YIVPDVVVHKMGNEYVVVLNEDGVPRLRVNSLYRSLLRSSAGSGDDAKQYVEQKLRSA
                     MWLIKSVDQRQRTLRKVTQSIVKFQREFLDKGLTYLRPLSLRDVGEDIGMHESTISRV
                     TTNKYVETPQGLFELKFFFHSGIASGDGEMVSSVSVKKMIQDLLANEDPSKPLSDQEV
                     AQILKGRALVIARRTVAKYREELGILPSHQRRLAPKRR"
     gene            6968..7279
                     /locus_tag="VLF19_02785"
     CDS             6968..7279
                     /locus_tag="VLF19_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSE92204.1"
                     /translation="MAVIFSGRGLVLAPSFRVRVEHKPARSGRLLHGPGDARAVCEAE
                     KFRRIARLTVRARCHTLTGEAIAGDLLAVVDGTPDALRRQARETKERRRRSRSRAGRV
                     A"
     gene            7281..8150
                     /gene="rapZ"
                     /locus_tag="VLF19_02790"
     CDS             7281..8150
                     /gene="rapZ"
                     /locus_tag="VLF19_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSE92205.1"
                     /translation="MSESIAFVVITGMSGAGKSYAIKCFEDMGFFCVDNLPTTLMPTF
                     ADLVARSAQRVHRVALGVDVREGEYLPHLLDAIAELRQRRHQVEVLFIEASDEALVRR
                     YHETRRRHPLGGEGHVHDAIRVERKALAHMREIADRIVDTSALTVHQFRDLLLELYGA
                     PKERAGLQTSVISFGFKHGIPIDADLVFDVRFLPNPHFVDHLRPRDGRDAAVREFILR
                     HEESRELVRRLEDLLRFLLPAYQREGKAYLTVAIGCTGGRHRSVAIAEELKPLLEGLG
                     FAPSVIHRDIDRE"
     gene            8157..9206
                     /locus_tag="VLF19_02795"
     CDS             8157..9206
                     /locus_tag="VLF19_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HSE92206.1"
                     /translation="MLDDPAARSRVDTRGTAAVLAAFPAQCREAARLLPRPDPVLRAL
                     RAVVVAGMGGSASSGDLLAACAADRLDVPIVVHRGYGLPALASERDLVVASSYSGETA
                     EVVSAAEAALARGAALVVVTAGGRLAALAADRAVPRVTLPTGLMPRMALGYLFFPLLA
                     VLRAADRTVVKAAEADEALAVLEALAAELRPERPAADNEAKRLALAIGDRLPVVYGGP
                     TTGLVAYRWKTDLEENAKTFAAAGALPEMNHNEIEAWGGPPARGRHLVLLRDHDEHDE
                     IARRFALLHELIGGAAGGTSEVWPRGGGLLARLLSLVYLGQWTSYYLALLRGVDPWTI
                     PTLETLKARMRGAVR"
     gene            9275..10426
                     /gene="metK"
                     /locus_tag="VLF19_02800"
     CDS             9275..10426
                     /gene="metK"
                     /locus_tag="VLF19_02800"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSE92207.1"
                     /translation="MARDEYLFTSESVTEGHPDKIADQISDAVLDAIIAQDPIGRVAC
                     ETLLTTGLVVVAGEITTSCYVDIPRVARDTIRQVGYTRAKFGFDFETCGVITAIDEQS
                     SDIAMGVDNLGAGDQGLMFGYACTETPELMPLPIMLAHRLTMGLAQLRKTGILDYLRP
                     DGKSQVSVRYVDERPRAVETVVISTQHGPDVTTEQIREELIEKLIVPTIAAELLDRKK
                     CVFHVNPTGRFVTGGPMGDTGLTGRKIIVDTYGGSCPHGGGAFSGKDPTKVDRSACYM
                     ARHVAKNIVAAGLAERAQVQVAYAIGVADPVSIMVDTFGTGKVSNAKLEQMIRRHFDF
                     TPAGIIKYLNLRRPIYKKTAAYGHFGRSEPEFTWERTDRVKDLRDDAGI"
     gene            10592..11860
                     /gene="ahcY"
                     /locus_tag="VLF19_02805"
     CDS             10592..11860
                     /gene="ahcY"
                     /locus_tag="VLF19_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSE92208.1"
                     /translation="MVGHDVKDLGLAAAGKLKIEWAEQSMPVLRQVRERFAKEQPLKG
                     IRLGACLHVTTETAVLMLTLKAGGAQVALCASNPLSTQDDTAAALVKEYDIPVYARKG
                     EDHPTYYRHLEAVLAIQPQITMDDGADLISQLHKEGNRGGDLTAQVIGGTEETTTGVI
                     RLRAMEKEGVLAFPVVAVNDADTKHLFDNRYGTGQSTIDGILRATNVLLAGRTVVVSG
                     YGMCGRGVAARAKGMGAHVIVTEVEPMRALEAVMDGFPVLPMLRAAETGDVFVTVTGN
                     TGVIRKEHFTRMKDGAILANSGHFNVEIDLEALAAMAQGRRTVRPFVEEFQLTGGRRL
                     YVLGEGRLINLTSAEGHPAAVMDMSFANQALSAEFMVKRGKTLERKVYVVPREIDREI
                     ARLKLASMNVEIDELTAQQESYLASWRHGT"
     gene            11880..>13525
                     /locus_tag="VLF19_02810"
     CDS             11880..>13525
                     /locus_tag="VLF19_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HSE92209.1"
                     /translation="MDRSRAILFFVLALVVLGVVGQVVPLYTDWLWFQEVGYTQVFLT
                     TLSFRGGLFAAMAVAVLVFLYVNLTLATRTARSDVYWELEDQLGLPGRVVIEPLIRRF
                     MPIVIALISFVSGLRASAHWETVLEYLNAVPFGIEDPLFGRDLGFFVFNLPAWRLVAG
                     WATTLVVGTLLLTLAVYVLQRSLALTTRGPRLAAGARAHLLVLGALLLALAGVGFWLD
                     RYEVVYSARGVVFGATYTDVNATLPVLGVLAVLAGLAAASCLAQLPRPGLRIVAGGVV
                     LLVATWVVGLLIYPALLQRLRVTPNELAAERPFIVHNIRMTRQAYGLDRIVEREFPAD
                     ETLEARALERNAPTIRNIRLWDHRPLLRTFGQLQEIRTYYKFVDVDNDRYALGDEYRQ
                     IMLSPRELSYQHLQGGRSWINEHLTFTHGYGVVVGPVNRVTGEGLPEFMVKDIPPRSE
                     GFPNVTRPEIYFGELSNEYVVVRTRSQELDYPSGDQNVYTAYGGAGGIQLSSFLRKVA
                     FAARFAEAKILLSNDISGESRVLMHRSIGDRVRRIAPFFH"
BASE COUNT         2111 a         4739 c         4664 g         2011 t
ORIGIN      
        1 cgaaccgatg aagccgggcg agacacgctt catcttcgtc accggcggag tcgtttcctc
       61 gctcggcaag ggcctggcgg cggcctcgat cggctgtctg ctggaggcac gcggctttcg
      121 cgtcaccctg cagaagatgg acccctacat caacgtggac gcgggcacca tgagccccta
      181 tcagcacggc gaggtcttcg tcaccgacga cggcggcgag accgacctcg acctcggcca
      241 ctacgagcgg ttcacgtcgg cgcgggtgac ccgccagcac aacgtgacca ccggcaaggt
      301 gtacttctcg gtgatccaga aggagcgccg cggcgactac ctcggccgca cggtccaggt
      361 gatcccccac atcaccgacg agatcaaggc cgccatccgc cgcgtcggcg gcgacgtgga
      421 cgtcgtgatc gtcgaggtcg ggggcaccgt gggcgacatc gagagcctgc cgttcctgga
      481 ggccatccgc cagctcaaga aggacgtggg caaggacaac gtcatctaca tccatctcac
      541 gctcgtgccg ttcatccagg ccgcccagga gctgaagacc aaggccaccc agcactcggt
      601 gaaggagctg cgcgcgatcg gcatccagcc cgacgtgctg ctctgccgca cggatcggta
      661 cctgccgccc ggcatcaagt cgaagatcgc cctcttctgc gacgtcgagg aggaagccgt
      721 catcaccgcc aaggacgtgg acaccgtgta cgaggtgccg caggtgttcc accgcgaggg
      781 cctcgacgcc atcatcgtca agctgctggg gctgcccgac cggcccagcg acctctcgcg
      841 gtgggacgag gtcgtcaagc gcgtgaagtc gccgcgccac gagacgcgga tcgccgtggt
      901 gggcaagtac atcgagctgc gggactcgta caagagcctg gtcgaggcgc tcacccacgg
      961 cgggatcgcc aacgacgcgc gcgtggacct cggctgggtc gacgccgagc acatcgagcg
     1021 cgacggcgcg gccgcccact tcgccgacgt gcacggcatc ctcgtgcccg gcggcttcgg
     1081 cgaccggggc atcgagggca agatcgcggc cgttcggtac gcgcgcgagg ccggggtgcc
     1141 gtacttcggc atctgcctcg gcatgcagtg cgcggtcatc gagttcgcgc gccacgtgtg
     1201 cggcctcgac cgcgccaact caacggagtt cgacgccgcc accccgaatc cggtgatcga
     1261 cctgctgccc gagcagcggg cgatcgccgc caagggcggt acgatgcggc tcgggctcta
     1321 cccgatcgtg ctggccgagg gcagcatggc ggcacgcctc tacggccagt cgatcatcca
     1381 cgagcgacac cgccaccgct acgaggtcaa caacgactac ctgcagcggc tcgagaagaa
     1441 cgggctcagg gtgtcgggcg agtgggccga aaagggcatc gtcgagatga tcgagatccc
     1501 cgagcacccg tacttcgtcg ccggccagtt ccacccggag ttccggtccc gtccgtggga
     1561 tccgcacccg ctcttcgccg ggttcatccg cgccgcgctc gggcacgcgc gggcgaggcg
     1621 cagggcgtcc gcgccatgag cctctcatcc gccgtgcgca tcgggcccgt cgtcgtcggg
     1681 gccggccacc cgctggcgct aatcggcggg ccctgcgcca tcgagaacga gaagcacgcg
     1741 ctgatgacgg ccgagcggct ggccgcgatc gccgccgctc accgggtgcc gttcgtctac
     1801 aagtcgtcgt acgacaaggc caaccgctcc tcgatcgatg gctaccgcgg acccgggctc
     1861 gtcgagggcc tgcgcatcct ccgtcgggtc aaagaggtcg tcggcgtccc ggtgctctcg
     1921 gacgtccacc aggtcgagga agtcgagccc gccgccgagg tcctggacgt gctgcagatc
     1981 cccgcgttcc tctgccgcca gaccgacctc gtcgtcgccg ccgcccgcag cggccggccg
     2041 gtgaacgtca agaagggcca gttcgtcgct ccggacgaca tgaggaacgt catcgagaag
     2101 gtccgtgccg ccggcaacga ccaattgctc ctcaccgagc gcggtaccag cttcggctac
     2161 cacaacctcg tcgtcgacat gcgaggactc gtacagatgc gggacctcgg cgtgccggtc
     2221 gtgttcgacg ccacgcattc cgtccagctg ccgggcgccg gcggcagccg ctcgggcggt
     2281 gagcggcggt acgtacccgc gctcgcgcgg gccgccgtgg ccgtgggtat cgacgcgctc
     2341 ttcatggaga tgcacgagga cccggaccgc acgctggccg acggtcggcc gctctccgac
     2401 ggccccaaca tgcttcggat ggacgacctg ccgcgcctgc tcgacgagct cctcgcgatc
     2461 cgggccgccg tcctgcacac cggacgaggg gcgggaaccg aggcttccgg cgacgggacg
     2521 cgccggtgaa gagccaggca tgagcgacgt ccggcggcgg gccgcgcgcg tcctgcgcat
     2581 ggaggccgag gcgatccttg ccctggtgcc gcggctcgac gagcgcttcg accgggcggc
     2641 ggagctgctc taccgctgca cgggccgcgt ggtcgttgcc ggcatgggca agtccggcca
     2701 tgtcgggcgc aagatcgccg cgaccctcgc ctccaccggc accccagcct acttcctgca
     2761 tcccgccgaa ggcatgcacg gcgacctcgg catgctcgcg ccgggcgacg tcgtgctggc
     2821 cctgtccaac tctggcgaga ccgacgagat gctggcgatg ctgccgacgt gcaagcgcct
     2881 cggcgtgccg atcgtgctcc tcaccggcaa cccgaagtcg acgctggccc gacagtgcga
     2941 cgtcgtcctc gacgtgagcg tggccgagga ggcgtgcccg atgaacctgg cgccgacgtc
     3001 cagcaccacg gcggccctgg ccgtcggcga cgccctggcg atggccctgc tcgacctgcg
     3061 cggactgcgt cctgagcact tcgccgcgtt gcatccccgc ggcacgctcg gctggcgcag
     3121 cctcatccgc gtgggcgacc tcatgcacgc gggtcccgag atgcccgtcg tcagtgccga
     3181 cacccccatg aaggacgtca tcgtggagat gaccgacaag cgactgggca tgaccacggt
     3241 cgtcgacgac caggggcgcc tgatgggcgt gatcacggac ggcgatctga ggcgggcgca
     3301 cctccgcgcc gagcccatcg ccaacctcac ggccgccgcg ctcgcgacgc gccgcgactt
     3361 ccaccgcgac ggcgatccgc gaaccaagac gatccaggcc gaagacctgg ccgcgaaggc
     3421 gctcgagatc atggagaccc acacgatcac gagcctggtc atcgtcgatc ccgcgcagcg
     3481 tcccgtcggg atcatccatt tacacgacgt cctgcgcgcc aagatcgacg tctagggcgt
     3541 cagggcgttt tacttttcct cggctctcga ccctgtggta ggctggtacc gaaattgcag
     3601 gtacctcgat ggatcgccta gcacgtcgga tcttcgtgat cgtcgccctg ttcgtgctcg
     3661 tggtgacggg cacgctggtg gtgcggagcc gtgccatgcg cacggaggcc gtcggcccca
     3721 cgccctccgc agccgatctc agcatcaggg aagtggagct gcaggaagag actgccgacg
     3781 gggcacgctg gcagctcgtg gccgaccagg cgcaggtctt cgacgcggag ggacggacgg
     3841 cgcttcgcaa tgtgcgggtg cgcgtgcagg accgcgagcg cacgtggacc atcaccggtg
     3901 aggaaggcga ttacttcaag accgccaagg acttcgagct ccgcggcagc gtggtcatcg
     3961 tctcggacga cggcttgcgg ctggagacga cggtactgcg ctggaaaggt gatgggcggc
     4021 gcctgtggac ggacgcgccg gtgcgcatca cgcgggacgg cacggtgatc gagggcacgg
     4081 cgctcgagat gaagatggcc gaagaggcca ccaccgtggg agggcgcgtg cgcgcggtct
     4141 tccagccggg gcagcgctcg tgagggcccg cgccgtggcc gggctcgccc tgacggccgc
     4201 gctgctcgct ccgtccggcg tcgcggggca ggcctcgcgg ccggccccga agagccaggg
     4261 accggccccg ctacgaccca agtcgcccga ggcccgcgcg cagccggtga cggttcacgc
     4321 cgacaagatg gaccgctacg gcaaggagag cctgatcatc ttcacgggcc aggtcgtcgc
     4381 gcgccaggag ggctcggtgc agactgccga ccggatggag gtctacctcg acgaaaaggg
     4441 agaccggatc ctgcggacgg tctccaccgg caacgtccgg atcgtcaccg gcgactgccg
     4501 gatcggcacc tcgcaccgcg ccgagtacga cgacctcgag cagcgcatgg tgctcatcgg
     4561 cgacgcacgc gtgtggcagg acgacaacgt ggtcaccggg gacacgatca cgctcttcct
     4621 gtcccaggac cgcagcgtcg tgcagggcgg caagcaggag cgggtgacgg cggtcttcta
     4681 tccgaagccc caggacggtc ccgggacgcc gcggccgacc gcgcggcaat cctgcgagtg
     4741 accccatgga aggcctcgtc gcggacggtc tcaccaagtg gttcaagcac cgcaaggtgg
     4801 tggaccgtgt ctcgctcgac atccagcgcg gcgaggtcgt gggcctgctg gggccgaacg
     4861 gcgccggcaa gacgacgtcc ttctacatga tggtcggcct gctccccgcg gacggcggcc
     4921 gcatcttcct cgagggccgg gagatcaccc gcctgcccat gtaccagcgc tgtcgcctgg
     4981 gcgtggggta cctgccgcag gagtcgtcgg tgttccgcaa gctcaccgtc gaggagaatc
     5041 ttctggcgat cctggagacc ctcgacctca gcgccgccga gcggcgcgag cgggcgcagg
     5101 agctgctggc cgagcttgac ctcaccgcgc tggcgccgta tcccgcctac acgctgtccg
     5161 ggggggagcg gcgacgcctg gagatcacgc gcgcgctcgt cacgtcgccg cagtacctgc
     5221 tgctcgacga gccgttcacc ggcatcgacc cgatcgccat cggtgacatc caggagatcg
     5281 tgggccgcct gcgcgagcgg ggcatcggaa tcctgatcac cgaccacaat gtgcgggaga
     5341 cgctcgcgat caccgaccgg gcgtacattc tctacgacgg caaggtgctg gtgtccggca
     5401 ctgccagcga gatcgccaac aatccgaccg cccgggagat ctatctcggc gaccggtttt
     5461 ccctctaggt ccgagagagt ccgaccatgg ccatggaaac gcgcctttcc ctccggcaga
     5521 gccagcgggt ggtgatgacc ccgctgctcc agcaggcgat ccagctgctc cagctctcga
     5581 cgctcgagct gcaggaggtc gtgcagaagg agctgctgga gaatccgctg ctggaagagg
     5641 ttcccacgga caacgcggac gcccccgagg ccgccgcgac cgcgccggac gcaccggcgc
     5701 cggcgccccc ggcggccgag ccggcgcccc tcgagccgcc gccggtggac aaggagcggg
     5761 gcaccgacga cctgcccttc gatctcaccg cggtgatgtt cgacgaccac gaggagcgtt
     5821 cgctcgtggc ccaggaggag cgcgacgacc tgccgttcga gaacatggtc cgatcgcaat
     5881 cctcgcttgc cgaccatctc gacgagcagc tgcgcatggt cacggaggac acgctggtgc
     5941 ggcgcatcgg caccgagatc atcggcaacc tcgacgagga cggctacctc cgcgccgagg
     6001 tcgcggagat cgcccagcgc tgccagacga cggccgagga ggtcgagaag gtcctggtcc
     6061 tggtgcaggg cttcgacccc ccgggcgtgg ccgcccgctc ggtccaggag tgcctgctca
     6121 tccagctgaa gagcgacccg aaccccgatc cggtgtcggt cgagatcgtc gagcagcact
     6181 tcgacgatct ctcacgacgc cgttaccccg acatcgcccg cgcactgaag ctgccgctcg
     6241 accgaatcat ggagtcggtc gaggagatca tgggtctcga gccgaagccc ggccggcgct
     6301 tcggcggcag cgactcgcgc tacatcgtcc cggacgtcgt cgtccacaag atgggcaacg
     6361 agtacgtggt cgtgctcaac gaggacggcg tcccgcggct tcgcgtgaac tcgctgtacc
     6421 ggtcgctgct gcggagctcc gccggctccg gcgacgacgc gaagcagtac gtggagcaga
     6481 agctgcgctc ggcgatgtgg ctgatcaaga gcgtggacca gcgtcagcgc acgctgcgca
     6541 aggtgaccca gtccatcgtg aagttccagc gcgagttcct cgacaagggt ctgacgtacc
     6601 tccggccgct gtcgctccgg gacgtgggtg aggacatcgg catgcacgag tccacgatca
     6661 gtcgcgtcac gaccaacaag tacgtggaga cgccccaggg cctgttcgag ctgaagttct
     6721 tcttccacag cggcatcgcc tccggtgacg gcgagatggt gtcgtcggtc tcggtgaaga
     6781 agatgatcca ggacttgctg gccaacgagg acccgtccaa gccgctctcc gaccaggaag
     6841 tcgcccagat cctcaagggc cgagccctcg tcatcgctcg ccgcacggtg gcgaagtacc
     6901 gggaggagtt ggggatcctg ccctcccacc agcgtcggct ggcgccgaag cggcgctgag
     6961 gaacgtcatg gccgtgatct tcagcggccg cgggctcgtc ctcgccccgt cgttcagggt
     7021 gcgcgtcgag cacaagccgg ccaggtccgg ccgcctgctg catggacccg gcgacgcgcg
     7081 cgcggtgtgc gaggccgaga agttccgcag gatcgcccgg ctcaccgtcc gcgcgcgctg
     7141 ccacacgctg accggtgaag ccatcgccgg cgatctgctc gccgtcgtgg acgggacgcc
     7201 cgacgcgctg cggcggcagg cccgcgagac gaaggagcgc cggcggcggt cgaggagccg
     7261 ggccgggcgg gtggcgtgac gtgagcgagt ccatcgcgtt cgtcgtcatc accggcatga
     7321 gcggagcggg caagagctac gccatcaagt gcttcgagga catgggcttc ttctgcgtgg
     7381 acaacctgcc gacgacgctc atgccgacgt ttgccgatct cgtcgcgcgc tcggctcagc
     7441 gagtgcaccg cgtcgccctc ggcgtggacg tgcgcgaggg cgagtacctg ccgcacctgc
     7501 tcgacgccat cgccgagctg cggcagcggc gccaccaggt cgaagtgctg ttcatcgagg
     7561 ccagcgacga ggcgctggtg cggcggtacc atgagacccg gcggcgtcac cccctgggcg
     7621 gcgaggggca cgtgcacgac gccatccgcg tggagcgcaa ggcgctcgcg cacatgcggg
     7681 agatcgccga ccgcatcgtg gacacgtccg cgctcaccgt ccaccagttt cgcgacctcc
     7741 tgctcgagct gtacggcgcg ccgaaggagc gggccgggct gcagacgtcg gtgatctcgt
     7801 tcgggttcaa gcacggtatc cccatcgatg ccgacctggt gttcgacgtt cgcttcctgc
     7861 ccaacccgca cttcgtcgat cacctgcggc cgcgcgatgg acgcgacgcc gccgtgcgcg
     7921 agttcatcct gcgccacgag gagagccgcg agctggtgcg ccgcctggag gacctgctgc
     7981 gcttcctcct gcccgcgtat cagcgggaag gcaaggccta tctcaccgtg gccatcggct
     8041 gtaccggagg gcgacaccgc tcggtggcca tcgccgagga gctgaagcct ctgctcgaag
     8101 ggctcgggtt cgcgccgagc gtgatccacc gggacatcga ccgcgagtag cccgtggtgc
     8161 tcgacgaccc cgccgcccgt tcacgcgtcg acacccgcgg caccgcggcc gtgctcgccg
     8221 cgttcccggc gcagtgccgc gaagcggccc ggctccttcc acggccggat ccggtgctgc
     8281 gcgccctgcg cgcggtcgtg gtcgccggca tggggggctc ggcgtcgagc ggcgatctgc
     8341 tcgccgcgtg cgccgcggac cgcctcgacg tgccgatcgt tgtccaccgg ggctacggac
     8401 tgccggcgct ggccagcgag cgggatctgg tcgtggcgtc gtcgtactcg ggcgagacgg
     8461 ccgaggtcgt gtccgctgcc gaggcggcac tggcgcgcgg cgcggcgctg gtcgtggtga
     8521 ccgccggcgg ccggctggcc gcactggccg cggatcgggc ggtcccccgg gtgacgctgc
     8581 ccacgggcct catgccgcgc atggcactcg gttacctgtt cttcccgctc ctggccgtgc
     8641 tgcgggcggc cgaccggacc gtggtgaaag ccgccgaggc cgacgaggcg ctcgcggtcc
     8701 tggaggcgct cgccgccgaa ctccgcccgg agcgtccggc ggccgacaac gaggccaagc
     8761 ggctggctct ggcgatcggc gaccgcctgc cggtcgtcta cggcggtccc acgacgggcc
     8821 tcgtcgcgta ccgctggaag acggacctcg aggagaacgc caagacgttc gcggccgccg
     8881 gcgcgctgcc cgagatgaac cacaacgaga tcgaggcatg gggagggccg ccggcgcgcg
     8941 gccgtcacct cgtgctgctc cgcgaccacg acgagcacga cgagatcgcc cggcgcttcg
     9001 cgctgctgca cgagctgatc ggcggcgcgg cgggcggcac gagcgaggtg tggccgcgcg
     9061 gcggcgggct gctggcgcgc ctgctctcgc tcgtctacct ggggcagtgg acgagctact
     9121 acctggcgct gctccgcggc gtcgatccgt ggacgatccc gacactggag accctcaagg
     9181 cgcggatgcg cggcgccgtg cgctgaaagc gctgaaatgc tccgcgcccg cgtgctacac
     9241 tgtcacgact tttttgggcc gggagggcga gcggatggcg cgcgacgagt acctgttcac
     9301 gtcggagtcg gtaaccgagg gccaccccga caagatcgcc gatcagatct ctgacgcggt
     9361 cctggacgcg atcatcgccc aggaccccat cggccgcgtg gcctgcgaga ccctgctcac
     9421 cacggggctg gtggtggtcg ccggcgagat caccacctcc tgctacgtcg acatcccgcg
     9481 ggtcgctcgc gacacgatcc ggcaggtcgg ctacacccgg gccaagttcg gcttcgactt
     9541 cgagacgtgc ggggtcatca cggcgatcga cgagcagtct tcggacatcg ccatgggcgt
     9601 cgacaacctc ggcgccggcg accagggcct catgttcggc tacgcctgca ccgagacgcc
     9661 cgagctgatg ccgctgccga tcatgctggc gcaccgcctg accatgggtc tcgcgcagct
     9721 ccgcaagacg ggcatcctcg actacctgcg gccggacggc aagtcccagg tctcggtgcg
     9781 ctacgtcgac gagcggccgc gggcggtgga gaccgtcgtc atctcgaccc agcacggtcc
     9841 cgacgtgacc accgagcaga tccgcgagga gctcatcgag aagctcatcg tgccgacgat
     9901 cgccgcggag ctgctcgacc gcaagaagtg cgtcttccac gtgaacccga cagggcgctt
     9961 cgtcacgggc ggaccgatgg gtgacaccgg actcaccgga cggaagatca tcgtggacac
    10021 ctacggcggc tcgtgcccgc acggcggggg cgcgttctcg ggcaaggatc cgacgaaggt
    10081 ggatcgctcg gcgtgctaca tggcccgcca cgtggccaag aacatcgtcg cggcgggcct
    10141 cgccgagcgg gcccaggtgc aggtggcgta cgccatcggc gtggccgatc ccgtctcgat
    10201 catggtggac acgttcggga ccggcaaggt gtcgaacgcg aagctcgagc agatgatccg
    10261 ccgccacttc gatttcacgc cggccggtat catcaagtac ctcaatctcc ggaggcccat
    10321 ctacaagaag accgccgcgt atgggcactt cggccggtcc gagcccgagt tcacgtggga
    10381 gcgcacggac cgggtcaagg atctgcgaga cgacgccggc atttgactcg gaggggggct
    10441 tgccccccac ccagtcggag gggcctcgac ggcccttccg agatctcccc aggaccgggg
    10501 gttgcgcggg caaagcccgc gcgcgaagcg cggttgacct ccagcgcggc ggctgacccg
    10561 ccggtttcgt gaaggaaggg acagaggggc gatggtcggg catgacgtga aggatctggg
    10621 gctggcggcg gccgggaagc tgaagatcga gtgggcggag cagtcgatgc cggtgctccg
    10681 gcaggtgcgg gagcggtttg ccaaggagca gccgctcaag ggcattcgcc tcggcgcgtg
    10741 cctgcacgtg accacggaga cggccgtcct gatgctcacg ctcaaggccg gcggcgccca
    10801 ggtggcgctc tgcgcctcga accccctctc gactcaggac gacacggcgg ccgcgctcgt
    10861 gaaggagtac gacatccccg tctacgcccg gaagggcgag gaccatccga cctactaccg
    10921 ccacctggag gccgtgctcg ccatccaacc tcagataacg atggacgacg gcgccgacct
    10981 catctcgcag ctccacaagg aaggcaatcg cggcggcgac ctgaccgcgc aggtcatcgg
    11041 cggcaccgag gaaaccacga ccggcgtcat ccggctgcgc gcgatggaga aggaaggcgt
    11101 gctcgcgttc ccggtcgtcg ccgtcaacga cgccgacacc aagcacctgt tcgacaaccg
    11161 ctatggcacc ggccagtcca ccatcgacgg aatcctgagg gccaccaacg tcctgctcgc
    11221 cggccgcacc gtcgtcgtgt ccggctacgg catgtgcggc cgcggcgtgg ccgcgcgggc
    11281 caagggcatg ggggctcacg tcatcgtcac cgaggtggag cccatgcgcg cgctggaagc
    11341 tgtcatggac ggcttcccgg tgctgccgat gctcagggcc gccgagacgg gtgacgtctt
    11401 cgtgaccgtc accggcaaca cgggggtgat ccgcaaggag cacttcacgc ggatgaagga
    11461 cggcgcgatc ctcgccaact cgggccactt caacgtcgag atcgacctcg aggccctggc
    11521 cgccatggcg cagggacgtc gcacggtgcg gccgttcgtc gaggagttcc agctgacggg
    11581 cggccgccgc ctctacgtgc tgggggaggg gcgtctgatc aacctcacct cggccgaggg
    11641 gcatcccgcc gcggtgatgg acatgagctt cgccaaccag gccctgtcgg ccgagttcat
    11701 ggtcaaacga ggcaagacgc tcgagcgcaa ggtgtacgtg gtgccgcgag agatcgaccg
    11761 cgagatcgcc cggctcaagc tcgcctcgat gaacgtcgag atcgacgagc tcacggcgca
    11821 gcaggagtcc tacctcgcct cctggcgcca cgggacctag accccgagcg accggaccca
    11881 tggaccgttc ccgggcgatc ctcttcttcg tcctggcgct ggtggtcctc ggggtggtcg
    11941 gccaggtggt gccgctctac accgactggc tctggttcca ggaggtcggg tacacgcagg
    12001 tctttctcac cacgctgtcg ttccgcgggg gcctcttcgc ggccatggcc gtcgcggttc
    12061 tcgtcttcct ctacgtgaac ctcacgttgg cgacccgcac ggcgcgctcc gacgtgtact
    12121 gggagctcga ggatcagctc ggcctgcccg ggcgcgtggt catcgagccc ctcattcgac
    12181 gcttcatgcc gatcgtcatc gcgctgatct cgttcgtctc gggactgcgc gcgagcgcgc
    12241 actgggagac cgtgctcgag tacctgaacg cggtgccctt cggcatcgag gatccgctct
    12301 tcggccgcga cctgggcttc ttcgtgttca acctgccggc gtggcgcctc gtggcgggct
    12361 gggccaccac gctcgtggtc ggcacgctgc tgctgacgct ggcggtctac gtgctccagc
    12421 gcagcctggc cctcaccacg cgcggtccgc ggctcgccgc cggcgcccgc gcccacctgc
    12481 tcgtgctggg cgcgctcctg ctggccctgg ccggcgtcgg cttctggctc gaccgctacg
    12541 aggtggtcta ctcggcgcgc ggcgtcgtgt tcggcgcgac ctacaccgac gtcaacgcga
    12601 ccctgcccgt gctgggcgtg ctcgccgtgc tggccggcct cgccgccgcc tcctgtctcg
    12661 cgcagctccc gcggccgggg ctgcgcatcg tggccggcgg cgtggtgctg ctcgtcgcca
    12721 cctgggtggt cgggctcctg atctacccgg cgctgctcca gcggctccgc gtgacgccca
    12781 acgagctggc cgccgagcgg cccttcatcg tccacaacat ccggatgacg cggcaggcct
    12841 acgggctcga ccgcatcgtg gagcgcgagt ttccggccga cgagacgctc gaggcccgcg
    12901 ccctcgagcg caacgcgccg acgatccgga acatccgtct ctgggatcat cgcccgctgc
    12961 tcaggacatt cggccagctg caggaaatcc gcacctacta caagttcgtg gacgtcgaca
    13021 acgaccgcta cgccctgggc gacgagtacc gtcagatcat gctctcgccc cgtgagctgt
    13081 cctaccagca cctccaggga ggacggagct ggatcaacga gcacctgacg ttcacccacg
    13141 gctacggcgt ggtcgtgggt ccggtgaacc gcgtgaccgg ggaggggttg cccgagttca
    13201 tggtgaagga catcccgcca cgcagcgagg gtttcccgaa cgtcacgcgg ccggagatct
    13261 acttcggcga gctgtccaac gaatacgtgg tggtccggac gcgatcccag gagctcgact
    13321 acccgtccgg cgaccagaat gtctacacgg cgtacggggg ggcgggtggc atccagctgt
    13381 cctcgttcct caggaaggtc gcgttcgccg cccgcttcgc cgaggcgaag atcctcctgt
    13441 cgaacgacat cagcggcgag agccgggtgc tcatgcatcg ctcgatcggc gatcgcgtcc
    13501 ggcggatcgc gcccttcttc cacta
//
LOCUS       DATIAZ010000083         5604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_414069, whole genome shotgun sequence.
ACCESSION   DATIAZ010000083 DATIAZ010000000
VERSION     DATIAZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414069"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(554..877)
                     /locus_tag="VLF19_02815"
     CDS             complement(554..877)
                     /locus_tag="VLF19_02815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HSE92210.1"
                     /translation="MIAMWVKARVKPEGRDRFLKAIEVDALGSEGDEPGCYRFNVLRD
                     AKDPDVYYFYEVYEDDAALEKHRAMPHYAVWRAAIDTLDGPIEAVRCESVFPADRAYW
                     TRNKR"
     gene            complement(908..1846)
                     /locus_tag="VLF19_02820"
     CDS             complement(908..1846)
                     /locus_tag="VLF19_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSE92211.1"
                     /translation="MATFTPKLTEIASGLRFPEGPVAMADGSVVLVEIERRALSRVTS
                     DGKIHVIATLGGGPNGAAMGPGGKIYVTNNGGLKFVERPGKLFPVVQADDYAGGSIQV
                     VDLETGRFETLYDACEGRRLRGPNDLVFDGAGGFWFTDLGKTRDRDADRGAVYYAKAD
                     GSMIREAIFPLERPNGIGLSPDERTLYVVETPTARCWAFRLSAPGQIESANGPYRGEK
                     GRVVAGLGGYQMFDSLAVDGEGHVCVATLITGAVSDIWPDGSRVDQYTLPDMMVTNVC
                     FGGPGLRTAYATLSMGGTLVSFEWPRPGLPLHHLNR"
     gene            1894..2301
                     /locus_tag="VLF19_02825"
     CDS             1894..2301
                     /locus_tag="VLF19_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSE92212.1"
                     /translation="MIESDSAMELAARRLTGVVVLSPVGRIGHDEAEPFRHALEAYLD
                     ACSAGSDAVVLDLAGVEYVSSAGLRALLLAARQVKAQGGTLVVAAMQPFVQEVFEISR
                     FTLLFRTFASVRDALVELSPAAVAAFEAGRPRG"
     gene            2298..3257
                     /locus_tag="VLF19_02830"
     CDS             2298..3257
                     /locus_tag="VLF19_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE92213.1"
                     /translation="MIVRFWGTRGSIPISLTTAGIRRKLVAALVLAAGRSLDTPDKAR
                     AFVEGELSFPVSQTFGGNSACVEIETGGGEYVLCDMGSGARPFGLGVLARHGSARPQT
                     FHVFMSHLHWDHIMGFPFFPPAYIPGTRIRFYGCHPSMEEAFRRQHGAPSFPVEFSRL
                     SATIEFVRLVPGRTDEVAGFRVTPKTQRHSGDSYGYRFEREGRSLVYSTDSEHKLDDP
                     EDARGFVEFFRDADLVIFDAQYSLAEAMSVKEDWGHSSNIVGVELCQMAGARHLCLFH
                     HEPVLDDEEIAAVLQETRRLEEITRGDRPLQISAAYDGMEITL"
     gene            3320..5392
                     /locus_tag="VLF19_02835"
     CDS             3320..5392
                     /locus_tag="VLF19_02835"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HSE92214.1"
                     /translation="MAAVVAVLLASEVGPLHAVRLAALDAYQQLSPRVPGSAPAVIVA
                     IDDASLARHGQWPWPRTVLARLLLGIAEGGAAAVGIDIIMAEADRLSPGRLPALIDGL
                     EAEVARRLVALPSNDAVLGGVIRGIPTVVGVAGIEGDRALADGRRTAFRIFGEDPQRF
                     TRRFGGVLRSVPEIDGAATGHGLINPDVHSRVVRRLPLVAVVGTELMPSFALELLRVA
                     ARQPALSIRAGRRGIESVGVGDVVVPTEPDGAVWVHYGPSDPARFVSAADVLAGRDAQ
                     RFTRRLVLVGVTAVGLGDNHPTPVAARMPGVEIQAQLLENIFDGEHLTRPRWASWVEA
                     STVAAGGLVILGAAAALPVTGALLAAVAMVAATVGAGWLAYLWYGLLLDVALPVGGLS
                     LVFAVALGVGLAEARSQRRVLQRQLQAEREAAARVAGELAAARRIQMGILPSATALAA
                     DERLSVWACLEPARAVGGDLYDFFPVDRDRLVFLVGDVSGKGVAGSLFMAVSKALCKS
                     SVLRLQGDVAAAMREANAEIARDNREALFVTAWAAVLDVRTGSLQYCSAGHEPALVLG
                     AGAPRRLGRDSGPPLCVLDDFPYVAASCTLRPGEIICLITDGVTEAMNTAGELYGRDR
                     LEAVVAGPGPTATAVEIGEAIRRDVARFSAGTEPSDDVTILVVQWRGPGPGATAGGAA
                     ASAAISGS"
     gene            complement(5379..>5604)
                     /locus_tag="VLF19_02840"
     CDS             complement(5379..>5604)
                     /locus_tag="VLF19_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSE92215.1"
                     /translation="AEVVVVGHTDRVGSVQQNDALSLQRAERIRGELLQLGLPAHQVG
                     VAGRGEREPLVQTEDEVPEPRNRRVELTIR"
BASE COUNT          743 a         1967 c         2044 g          850 t
ORIGIN      
        1 ctgaaggagc gctaccagct gacggtgctg atggccgagc agaacttcaa ccaggccacc
       61 aagatcgccg accgcggcta catcatcgtg cacgggaaga tcgagttcga aggcacgagc
      121 acgcgggagc tgcgcgagaa cgagctggtg aagaagtact atctcggcgt gtgatcgctc
      181 gctcgggacg cccgatcttt ccccgtcgag ccggcctgcg gtacgctggg gtcgggtgcc
      241 atgggctcgg atacggggga tgacgatcaa gctctcagcg aggttcgcgc ggcaggaaag
      301 tgaagagacg cgtcgagcgc ggatcgcgcg gcgacgcata cgcgaagatc gggtcgaggc
      361 cgtccggcgg atgctggggc tgcctgacca ggcccgaacg ccgccaccgc cccggccgcg
      421 tcgtgtgagc accggcgaac gggtgctcga gtgcccgcgg tgcgatcgtc ggttcgctcg
      481 gcccatgcac ctcggcaggc acctgcaagc cgcgcacgcc tgaagcagaa ggcctgccgg
      541 gaaacaggtt gcgctagcgc ttgttcctcg tccagtaggc gcggtcggcg gggaacaccg
      601 actcgcagcg caccgcctcg atgggcccgt cgagcgtgtc gatggccgcc cgccagacgg
      661 cgtagtgcgg catcgcgcgg tgcttctcga gcgccgcatc gtcctcgtag acctcgtaga
      721 agtaatagac gtcggggtcc ttcgcgtcgc gcagcacgtt gaaccggtag caacccggct
      781 cgtcaccctc ggagccgagc gcgtccacct cgatggcctt caggaagcgg tcccggcctt
      841 ccggcttcac gcgcgccttc acccacatgg cgatcatcgg atctcccttc gagctcgcgg
      901 ttcggagcta gcggttcagg tggtgcagcg gcaggccggg gcgcggccac tcgaacgaca
      961 ccagggtgcc gcccatcgag agcgtcgcgt aggccgtgcg gaggcccgga ccgccgaagc
     1021 acacgttggt gaccatcatg tcgggcagcg tgtactggtc gacgcggctg ccgtcaggcc
     1081 agatgtcgct cacggcgccg gtgatcagcg tggccacgca cacgtggccc tcgccgtcca
     1141 cggcgaggga atcgaacatc tggtagccgc cgaggccggc gacgacccgg cccttctcgc
     1201 cgcggtaagg gccgttcgcg gactcgatct ggccgggcgc ggagaggcgg aaggcccagc
     1261 agcgcgcggt cggcgtctcc accacgtaga gggtccgctc gtcgggggag agcccgatgc
     1321 cgttcggccg ctccagcggg aagatcgcct cgcggatcat ggagccgtcg gccttcgcgt
     1381 agtagacggc gccgcggtcg gcgtcgcggt cgcgcgtctt gccgaggtcg gtgaaccaga
     1441 agccgccggc gccgtcgaac acgaggtcgt tcggcccgcg caaccggcgt ccctcgcagg
     1501 cgtcgtagag cgtctcgaag cggcccgtct cgaggtccac gacctggatc gacccgccgg
     1561 cgtagtcgtc ggcctggacg acggggaaca gcttgcccgg ccgctcgacg aacttgaggc
     1621 ccccgttgtt ggtgacgtag atcttcccgc ccggccccat cgccgctccg ttggggccgc
     1681 cgccgagcgt ggcgatcacg tggatcttgc catcggaagt gacgcgcgag agcgcgcggc
     1741 gctcgatctc cacgagaacg accgagccgt ccgccatcgc gacggggccc tccgggaacc
     1801 ggagacccga cgcgatctcc gtgagcttcg gcgtgaacgt ggccatgagc ggccgaacca
     1861 tagcgccgct gcgggggagg gtcaagccgc aggatgatag aatccgactc cgcgatggaa
     1921 ctggcggcca ggcgcttgac cggtgtcgtg gtgctgtcgc cggtgggtcg gatcggtcac
     1981 gacgaggcgg agcccttccg tcacgcgctc gaggcgtacc tcgacgcgtg ctcggccggc
     2041 agcgatgccg tcgtgctcga cctggccggc gtcgagtacg tctccagcgc ggggttgcgc
     2101 gcgctcctgc tcgcggcgag gcaggtcaag gcccaggggg gcacgctggt ggtcgcggcc
     2161 atgcagccgt tcgtgcagga agtcttcgag atcagccggt tcacgctgct cttccgcacg
     2221 tttgcctcgg ttcgcgatgc cctggtcgag ctgtcgccgg cggcggtggc ggccttcgag
     2281 gccgggcggc cgcgcggatg atcgtccgct tctggggcac ccgcgggtcc attcccatct
     2341 cgctgacgac cgccgggatc cgccgcaagc tcgtcgccgc gctggtgctc gcggccggcc
     2401 ggagcctcga cacgcccgac aaggcgcgcg cgttcgtcga gggcgagctg agcttcccgg
     2461 tatcgcagac cttcggcggc aattccgcgt gcgtcgagat cgagaccggg ggcggagagt
     2521 acgtgctgtg cgacatgggc agcggggcgc gcccgttcgg gctcggcgtg ctcgcacggc
     2581 acgggtcggc gcgcccccag accttccacg tgttcatgtc ccacctccac tgggaccaca
     2641 tcatggggtt tccgttcttc ccccccgcct acattcccgg cacccgcatc cgcttctacg
     2701 gctgccaccc gagcatggag gaggcctttc gacgccagca tggcgcgccg tcgttccccg
     2761 tggaattctc gaggctgtcg gcgacgatcg agttcgttcg cctcgtcccg ggccgcaccg
     2821 acgaggtggc ggggttccgc gtgacgccca agacgcagcg gcacagcggt gattcctacg
     2881 gctaccgctt cgagcgcgag ggccggagcc tcgtctactc gacggactcc gagcacaagc
     2941 tcgacgaccc cgaggacgcg cgcggcttcg tcgagttctt ccgtgacgcc gatctcgtga
     3001 tcttcgacgc ccagtactcg ctggccgaag cgatgtcggt gaaggaggac tggggacact
     3061 ccagcaacat cgtaggcgtc gagctctgcc agatggccgg cgcgcggcac ctctgcctct
     3121 tccaccacga gccggtcctc gacgacgagg agatcgccgc cgtgctgcag gagacgcgac
     3181 ggctcgagga gatcacgcgc ggcgatcgtc cactgcagat ctcggcggcg tacgacggga
     3241 tggaaatcac gctctgaccg cgccggcgct cgtcacccgt ctgcgaggcg gccgcgtcct
     3301 cctgccggcg ctggccctcc tggccgccgt cgtcgccgtt ctgctcgcgt ccgaggtcgg
     3361 gccactccat gccgtccggc tggccgcgct cgacgcctac cagcagctct cgccgcgcgt
     3421 ccccggctcg gcgccggcgg tcatcgtcgc catcgacgac gccagcctcg cccggcacgg
     3481 ccagtggccc tggccgcgca ccgtgctcgc gcgcctgctc ctcggcatcg cggagggcgg
     3541 cgctgccgcc gtcggcatcg acatcatcat ggccgaagcg gaccggctct ccccgggccg
     3601 gctgcccgcg ctgatcgacg gtctcgaggc cgaggtcgcc cggcggctgg tggcgcttcc
     3661 gagcaacgac gccgtgctcg gcggcgtgat cagaggcatc ccgacggtgg tcggcgtggc
     3721 cgggatcgag ggggatcgag cgctcgcgga cggccgaagg acggcgttcc ggatcttcgg
     3781 cgaggacccg cagaggttca cccgccgctt cggcggtgtg ctcaggagcg tcccggagat
     3841 cgacggcgcg gcgaccggtc acggtctcat caaccccgac gtccactcgc gcgtggtgcg
     3901 gcgcctgccg ctcgtggccg tcgtgggcac cgagctcatg ccttcatttg ccctcgagct
     3961 gctccgcgtg gcggcacgcc agcccgcgct gtcgatccgc gccggccgtc gtggcatcga
     4021 gtcggtcggc gtcggcgatg tcgtcgtccc cacggagccc gatggcgccg tctgggtcca
     4081 ctacggcccg tccgacccgg cgcgcttcgt gtccgccgcc gacgtgctgg ccgggcggga
     4141 cgcccagcgc ttcacgcgcc ggctcgtgct cgtgggcgtg accgctgtgg ggctgggcga
     4201 caaccatccg accccggtgg ccgcgcggat gccgggcgtc gagatccagg cgcagctcct
     4261 cgagaacatc ttcgacggcg aacacctcac ccgaccccgc tgggcctcgt gggtggaggc
     4321 gtcgaccgtc gcggctggcg ggctcgtgat cctcggcgcg gccgcggcgc tcccggtgac
     4381 gggtgcgctg ctcgcggccg tcgcgatggt cgcggccacg gtcggtgccg gatggctcgc
     4441 gtatctgtgg tatgggctgc tcctcgacgt cgcgctcccg gtgggcgggc tgagcctcgt
     4501 gttcgcggtc gcgctcggcg tggggctcgc cgaggcgcgg agccagcgcc gcgtgctcca
     4561 gcgccagctc caggcggagc gtgaagcggc cgcccgggtc gcgggcgagc tcgcggccgc
     4621 ccggcgcatc cagatgggca tcctgccgag cgcgacggcg ctggccgcgg acgagcgcct
     4681 gagcgtgtgg gcgtgtctcg agcccgcgcg ggcggtcggc ggggatctct acgacttctt
     4741 cccggtggat cgcgatcgtc tcgtctttct cgtcggcgac gtctccggaa agggcgtcgc
     4801 gggcagcctc ttcatggccg tcagcaaggc gctgtgcaag agcagcgtgc tgcggctcca
     4861 gggcgacgtg gcggcggcca tgcgggaggc gaacgcggag atcgcgcggg acaaccgcga
     4921 ggcgctgttc gtcacggcgt gggccgccgt gctcgatgtg cgaacgggat cgctacagta
     4981 ctgcagtgcc ggccacgagc ccgccctcgt gctcggcgcc ggggcgccga ggcggctcgg
     5041 gcgggacagc ggcccgccgc tgtgcgtgct ggacgacttt ccctacgtgg ccgcgtcctg
     5101 cacgctgcgg ccgggcgaga tcatctgcct gatcacggac ggcgtcaccg aagcgatgaa
     5161 caccgcgggc gagctgtacg gacgggaccg cctggaggcg gtggtggcgg ggcccgggcc
     5221 gacggccact gccgtcgaga tcggcgaggc gatccgtcga gacgtggcgc gcttctcggc
     5281 aggcaccgag ccctcggacg acgtcacgat cctggtggtc cagtggcgcg gccccggacc
     5341 cggcgccacg gcgggtggcg cggccgcgtc ggcggcgatc agcggatcgt gagctccacg
     5401 cggcgattcc gtggctccgg cacctcgtcc tcggtctgga cgagcggctc ccgctcgccc
     5461 cggccggcca cgccgacctg gtgggccggc aggccgagct gcaggagttc cccgcgaatg
     5521 cgctcggccc gctggagcga gagagcgtcg ttctgctgaa cgctgccgac gcgatcggtg
     5581 tggccgacga cgaccacctc ggcg
//
LOCUS       DATIAZ010000084         5581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_151266, whole genome shotgun sequence.
ACCESSION   DATIAZ010000084 DATIAZ010000000
VERSION     DATIAZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151266"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VLF19_02845"
     CDS             <1..218
                     /locus_tag="VLF19_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92216.1"
                     /translation="EHLKARGMIVRLPHPTAGEVTVMGVPLRLHGTPGAVVSPPPRLG
                     EHTDAVLKRVLGMRPSQLRRLRAAAVL"
     gene            complement(292..510)
                     /locus_tag="VLF19_02850"
     CDS             complement(292..510)
                     /locus_tag="VLF19_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HSE92217.1"
                     /translation="MRGLALVGVVLIVLGAVALAYQGITYTTREKVVDLGPLKVEAQR
                     EKTIPLPPVLGGIAVAVGVVLLVMGLRR"
     gene            623..2206
                     /gene="cimA"
                     /locus_tag="VLF19_02855"
     CDS             623..2206
                     /gene="cimA"
                     /locus_tag="VLF19_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HSE92218.1"
                     /translation="MRSTVTIYDTTLRDGTQGEGVSFSMEDKVRLATRLDAIGVHYIE
                     GGWPGSNPKDLRFFRRMQDVPLKHAKLAAFSMTRRSGGTAESDANMRALIDAGAPVAT
                     IVGKSWDFHVTHALETTLAENLAMIADTIAYLCPRTDEVIYDAEHFFDGFRANRDYAL
                     ATLRAAEEAGAQWLVLCDTNGGTLPFELVEVLREVKRHVRAPLGIHVHNDAECAVANT
                     LAAVREGVRQVQGTVNGYGERCGNANLVSIVPSLVLKMGIDCLPQANLRELRDLSRFV
                     SELANRTPWPSQPYVGDSAFAHKGGMHVSAVVKHPETYEHVDPEAVGNHRRVLVSELS
                     GRSNILWKAKEYGLDLDRDTPDSRRILDRLKDLEDQGFLFEGAEASFELLMERALGRH
                     TAYFDLEAYRVIVEEQDGSSEPVAEATVRVRVKGMLEHTAAAGNGPVNALDHALRKAL
                     EEFYPNLREMRLLDYKVRILDESKGTAAKTRVLITSGDGRDTWGTVGVADNIIEASWL
                     ALVDSVEYKLRRDERASLR"
     gene            complement(2254..2715)
                     /locus_tag="VLF19_02860"
     CDS             complement(2254..2715)
                     /locus_tag="VLF19_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HSE92219.1"
                     /translation="MSAKQDLLSQAAREYKAFHEAIAGLNEAQMTEVWLGAWSVKDIV
                     AHMTAWHREMAPALERLARGERPLQEGVRYDDVDAWNARFVAAAREQSVADVLLDFDK
                     THETFMRAADGVPAGRWEPGKTAYRIVDQNSAHHYREHGEQIRAWRAERGL"
     gene            complement(2739..3161)
                     /locus_tag="VLF19_02865"
     CDS             complement(2739..3161)
                     /locus_tag="VLF19_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE92220.1"
                     /translation="MEIRHAQPPDLPAVMGLAAELIGPERAGPFVRSHVERHHLLVAD
                     SGESVVGVLAYRTDWFQCTFVSLVCVRESFRRQGVARALFKAVEQMSPSPRLFSSTEE
                     TNASSIRMHTALGFAPSGHIDNLPQGYRELLFYKRLAP"
     gene            complement(3239..4069)
                     /locus_tag="VLF19_02870"
     CDS             complement(3239..4069)
                     /locus_tag="VLF19_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE92221.1"
                     /translation="MPTNGVRLAVKVWEGGSGAKTIVAVHGLTANHTCWYPVAEYVVP
                     EFRLVAYDLRGRGDSDKPPKGYSLAEHARDLLGVLDRLGLERAVIMGHSLGAHIAVWF
                     GAHHPGRVDRLVLLDGGLDVRAEVFDSLAPAIDRLGVEFPSLDRFLAMLRGLPMFAGR
                     WNDHLTRYFTYDVEPGPAGGVRSKVARHAVEEELANLARTRLWVLHHTLAAPALLVRA
                     PDGLLTATDCLMTQDEAEAMAHAIPRCRLVVAPGTNHYTVLLSDEPVVKREVLDFLRG
                     "
     gene            complement(4078..4755)
                     /locus_tag="VLF19_02875"
     CDS             complement(4078..4755)
                     /locus_tag="VLF19_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HSE92222.1"
                     /translation="MIDPLRKISSPSVANAIETFNVRPRNQGNMSSEIRCLFPELGPL
                     VGYAVTAVIRAEPQPLQGHRASTFGWWDYVLSIPAPRVVVVHDLDEPRGQGAQWGEVQ
                     ANIHRALGCVGVVTDGSVRDLDEVRALGFQFAAAHVSVSHAYVHMVDFGLPVKLGGLW
                     VKPGDLIHADQHGAVTIPADIAAKIPEAVARVEADERKIIEICQSRDFSADKLKDLYK
                     VIRPGTY"
     gene            complement(4824..5309)
                     /locus_tag="VLF19_02880"
     CDS             complement(4824..5309)
                     /locus_tag="VLF19_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE92223.1"
                     /translation="MAEVPALVEALHDRDVLVRALAERALWEVWSRSGDGEADRLLAI
                     GIEQMSARRGEAAVETFSRVIELRPDFAEGWNKCATVYYLLGEYEKSLADCDEVVKRN
                     PYHFGALSGYGMIWLKLDEPEKALAWFERALDVNPNLESVEEAVQTLRRLLRERRKQG
                     I"
BASE COUNT          836 a         1972 c         1978 g          795 t
ORIGIN      
        1 gcgagcacct gaaggcgcgc ggcatgatcg tccgcctgcc gcacccgacg gcgggcgagg
       61 tgacggtcat gggcgtgccg ctgaggctgc acggcacgcc gggcgccgtc gtgtccccgc
      121 cgccgcgttt gggcgagcac accgacgcgg tgctcaagcg cgtcctcggc atgagaccct
      181 cccagctgcg ccggctccgc gccgccgccg tcctgtgatc ggaggggcct cgacggcccc
      241 ggccacctcc gatgaatcag gctccttccg gagaagcccc ctccgagcgg tctaacgtct
      301 gagacccatg acgaggagga cgacgccgac ggcgaccgcg atgccgccca ggacgggcgg
      361 caaggggatc gtcttctccc gctgcgcctc gaccttgagc ggaccgaggt ccacgacctt
      421 ctcccgggtg gtgtaggtga tgccctggta ggccagggcc accgcgccga gcacgatcag
      481 gaccactccc accagcgcca gtccacgcat gtcccacctc cggggctctc agtctcatcg
      541 tacccagccc ggcgcaatct tgacacccct tccggcgctg gctcatgatg tcgggacgca
      601 ttcgatcgag cggaggcagc cgatgcggtc cacggtcacg atctacgaca cgacgctgcg
      661 cgacggcacc cagggcgagg gcgtgtcgtt ctccatggag gacaaggttc gtctcgccac
      721 gcgcctggac gcgatcggcg tccactacat cgagggcggc tggccaggct cgaatcccaa
      781 ggacctccga ttcttccgcc gcatgcagga cgtgccgctc aagcacgcca agctcgccgc
      841 gttcagcatg acccggcgct cgggcgggac cgcggagagc gacgccaaca tgcgtgcgct
      901 gatcgacgcg ggcgcgccgg tcgccaccat tgtcggcaag tcgtgggact tccatgtcac
      961 gcacgcgctg gagacgacgc tggcggagaa cctcgcgatg atcgccgaca cgatcgccta
     1021 cctgtgcccg aggacggacg aggtcatcta cgacgccgag cacttcttcg acggcttccg
     1081 cgccaaccgg gactacgcgc tcgcgacgct gcgggcggcc gaggaggccg gcgcgcagtg
     1141 gctcgtactc tgcgacacca acggcggcac gctgcccttc gagctcgtgg aggtcctccg
     1201 cgaggtgaag cggcacgtgc gggccccgct cggcatccac gtccacaacg acgccgagtg
     1261 cgcggtcgcc aacacgctcg ccgcggtccg cgagggcgtg cgccaggtcc agggcacggt
     1321 gaacggctac ggcgagcggt gcggcaacgc gaacctcgtc tccatcgtcc cgagcctcgt
     1381 gctgaagatg gggatcgact gtctccccca ggcgaacctc cgcgagctgc gagacctgtc
     1441 gcgcttcgtc tcggagctgg ccaaccgcac gccctggccg tcgcagccct acgtgggcga
     1501 ctccgcgttc gctcacaagg gcggcatgca cgtctcggcc gtggtgaagc atcccgagac
     1561 ctacgagcac gtcgaccccg aggccgtggg caaccaccgg cgtgtgctcg tgtccgagct
     1621 gagcggccgg tcgaacatcc tgtggaaggc gaaggagtac ggcctcgacc tcgaccgcga
     1681 cacgcccgac tcgcgccgga tcctcgaccg gctcaaggac ctcgaggacc aggggttcct
     1741 cttcgagggc gcggaggcat cgttcgagct cctgatggag cgcgcgctgg gccggcacac
     1801 ggcgtacttc gacctcgagg cctaccgcgt gatcgtcgag gagcaggacg gctcgtcgga
     1861 gccggtggcc gaggcgaccg tgcgcgtgcg cgtcaagggc atgctcgagc acacggccgc
     1921 cgccggcaac gggcccgtca acgcgctcga ccacgcgctg cgcaaggcgt tggaggagtt
     1981 ctacccgaac ctgcgcgaga tgcggctgct ggactacaag gtgcggatcc tcgacgagtc
     2041 gaagggcacc gcagccaaga cccgcgtgct gatcacgtcg ggcgacgggc gcgacacatg
     2101 gggcacggtg ggcgtcgccg acaacatcat cgaggcgtcg tggctggccc tcgtggactc
     2161 cgtcgagtac aagctccgcc gggacgagcg cgcgagcctg cgctgacgcg cgacgcgcag
     2221 gtcctcaccg gcgccggtcg ccgatcgcgg acgtcagagt ccgcgctccg cgcgccaggc
     2281 gcggatctgc tcgccgtgct cccggtagtg gtgggcggag ttctggtcga cgatccggta
     2341 ggccgtcttg cccggctccc agcgccccgc cggcacgccg tccgcggccc gcatgaaggt
     2401 ctcatgggtc ttgtcgaagt cgagcagcac gtcggcgacc gactgctcgc gcgccgcggc
     2461 gacgaagcgc gcgttccacg cgtccacgtc gtcgtagcgg acgccttcct gcagcgggcg
     2521 ctcgcctcgg gcgaggcgct ccagcgccgg cgccatctcg cgatgccagg ccgtcatgtg
     2581 ggcgacgatg tccttgacgc tccacgcgcc gagccagacc tccgtcatct gggcctcgtt
     2641 gaggccggcg atcgcctcgt ggaacgcctt gtactcccgg gccgcctgac tcagcaggtc
     2701 ttgcttggca ctcacgacgc cctcctccgt cgagaccctc agggggccag acgcttgtag
     2761 aacaggagct cccggtaccc ctgaggcagg ttgtcgatgt ggcccgaggg cgcgaagccc
     2821 agcgcggtgt gcatgcggat cgaggacgcg ttcgtctcct cggtcgacga gaacaggcgc
     2881 gggctcggcg acatctgctc gacggccttg aagagcgcgc gcgccacgcc ctggcgccgg
     2941 aaactctcac gcacgcagac gagcgacacg aacgtgcact ggaaccagtc agtccggtac
     3001 gcgagcacgc cgaccaccga ctcgccggag tcggcgacga gcaggtgatg gcgctcgacg
     3061 tgcgagcgga cgaacgggcc tgcgcgctcg ggcccgatga gctcggccgc cagacccatc
     3121 acggccggca ggtcgggcgg ctgtgcgtgg cggatctcca tggcgccagt gtaccgccgg
     3181 ggttcccgcc gtgcgcacag tgggcgcttt cggcacgcgg caccccgtcg ggtcgccgtc
     3241 agccgcggag gaagtcgagc acctcgcgct tcacgacggg ttcgtcggac agcagcaccg
     3301 tgtagtgatt ggtgccgggc gccacgacga gacggcagcg ggggatggcg tgcgccatgg
     3361 cctccgcctc gtcctgggtc atgaggcagt cggtggcggt cagcagcccg tccggtgcgc
     3421 gcaccagcag cgctggcgcc gccagcgtgt ggtgcaagac ccagagccgc gtgcgcgcca
     3481 aattcgccag ctcctcctcg acggcgtggc gcgccacctt cgaccgcacg ccgccggccg
     3541 ggccgggctc gacgtcgtag gtgaagtagc gggtgaggtg gtcgttccag cggcccgcga
     3601 acatcggcag cccccgcagc atggcgagga agcggtcgag cgacgggaac tcgacgccga
     3661 gccggtcgat cgcgggggcc agcgagtcga agacctccgc gcgcacgtcg aggcctccgt
     3721 cgaggagcac cagacggtcc acgcgcccgg ggtgatgcgc gccgaaccac acggcgatgt
     3781 gggccccgag cgagtggccc atgatcaccg cccgctccag tccgaggcga tcgagcacgc
     3841 cgagcaggtc gcgcgcgtgc tccgccaggc tgtagccctt cgggggcttg tcgctgtcgc
     3901 cgcgcccgcg caggtcgtag gcgaccaggc ggaactcggg gacgacgtac tccgcgaccg
     3961 gataccagca ggtatggttc gcggtgaggc cgtggacggc gacgatggtc ttcgcgcccg
     4021 acccgccctc ccagaccttc acggcgagcc ggacgccgtt cgtcggcacg agctccatca
     4081 gtacgtaccg ggacggatca ccttgtacag gtccttcagc ttgtcggcgc tgaagtccct
     4141 cgactggcag atctcgatga tcttgcgctc gtcggcctcg accctggcca cggcctccgg
     4201 gatctttgcg gcgatgtcgg ccgggatcgt caccgcgccg tgctggtcgg cgtggatgag
     4261 gtcaccgggc ttcacccaga gcccgccgag cttgaccggc aggccgaagt ccaccatgtg
     4321 gacgtaggcg tgggagacgg agacgtgggc ggcggcgaac tggaagccga gcgcccgcac
     4381 ctcgtcgaga tcccgcacgg agccgtcggt gacgacgccg acgcagccga gcgcgcggtg
     4441 gatgttggcc tgtacctcgc cccactgggc gccctgaccg cgtggctcgt ccaggtcgtg
     4501 gacgacgacc acgcggggcg cggggatcga gagcacgtag tcccaccagc cgaacgtgga
     4561 agcgcgatgg ccctgcagcg gctggggctc ggcgcggatg acggcggtca ccgcgtagcc
     4621 gacgaggggt ccgagctcgg ggaacaggca gcggatctcc gaggacatgt tgccctggtt
     4681 cctcgggcgc acgttgaacg tctcgatggc gttggcgacg ctggggctcg agatcttgcg
     4741 gagcgggtcg atcacgttgc tcctcctgcc ttcggtggct tgcgtcggtg cgctgcgggg
     4801 tggccggggg cgcctgggtc gcgtcagatg ccctgcttgc gccgctcgcg gagcaggcgt
     4861 cgcagcgtct gcaccgcctc ctcgaccgat tccagattcg ggttcacgtc gagcgcgcgc
     4921 tcgaaccagg ccagcgcctt ctcgggctcg tcgagcttca gccagatcat gccgtagccc
     4981 gagagcgcgc cgaagtggta cggattgcgc ttcaccacct cgtcacagtc ggcgagcgac
     5041 ttctcgtact cgccgagcag atagtagacg gtcgcgcact tgttccagcc ctcggcgaag
     5101 tccggccgga gctcgatcac gcggctgaag gtctcgacgg cggcctcgcc ccgacgcgcg
     5161 ctcatctgct cgatgccgat ggcgagcagg cggtcggcct cgccgtcgcc cgagcggctc
     5221 cagacctccc acagcgcgcg ctcggcaagc gcccgcacga ggacgtcccg atcgtgcagc
     5281 gcctcgacca gcgcgggaac ctcggccatg gtcccgatct cgccgagcac ctgggcgccg
     5341 cgtcgccgag cctcgacgct ggcggcgtca cgcagctccg cgagtccgcg ctcgagtggt
     5401 agggcgagcc gagcggcgtc ctcgccgcga ggggagcggt gagatggttc ggaaaggagg
     5461 gcgagccgag cggcgtcctc gccgcgaggg gagcggggtt gggccggcgc gagcgcggga
     5521 gcgagcagcg ccacgcccag cgcggcggcc acgggcatcc cgttcacggc gcgtacacct
     5581 c
//
LOCUS       DATIAZ010000085        11943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1204888, whole genome shotgun sequence.
ACCESSION   DATIAZ010000085 DATIAZ010000000
VERSION     DATIAZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204888"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="tig"
                     /locus_tag="VLF19_02885"
     CDS             complement(<1..1196)
                     /gene="tig"
                     /locus_tag="VLF19_02885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSE92224.1"
                     /translation="MKVAVEEMEGCKRRVAVEAPADVVQKEWERAYGRVQKHARLPGF
                     RRGHVPRSLVKVHFADDVRREVAEHLIPDVYRRALTEVRLEPVADPDLQEVRLEEGAP
                     LSFVAVVEVKPAIELGDYRSVEVQHAPGSVTAEDVDATLERMRESQAQFVAVERAAGP
                     GDLVIVDYALTLDGQEPTRQMGYAFVVGEGSVLPEVDEAVAGMRAGEERSFGFRLADD
                     HRREELRGKSGTGTVKVSEVKEKVLPEVNDDLAKSLGEFDTLEALRAAVLRQLEARRA
                     AEDRHALEDKVIDTLLARHPFAVPEAMVMRHIAHQVEHARERLRRQGVDPDAMPWDYG
                     KLVTEMRPGAERGVRRALLLEAVAERESVAVTDADVDTEVESIASASQRPTPAIRRMM
                     EKSGDL"
     gene            complement(1382..1469)
                     /locus_tag="VLF19_02890"
     tRNA            complement(1382..1469)
                     /locus_tag="VLF19_02890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1433..1435),aa:Leu,seq:tag)
     gene            complement(1433..1972)
                     /locus_tag="VLF19_02895"
     CDS             complement(1433..1972)
                     /locus_tag="VLF19_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92225.1"
                     /translation="MQRGVGRRAPLAPVPDAPPESDPLAAVPPQTLPPDVVLETPLPS
                     GAPLPVADVESEDPVPPETPLPEPDVVPDIVLPVLLPLETPPPGVVLPVREPLGESAG
                     LKSASLRLPVARVEVRLGGVARPRQALHRDRGRGRAERCRTLLQKVYRLAGFCGRGYA
                     ARGVPHVAREWRNWQTRRT"
     gene            2132..2488
                     /locus_tag="VLF19_02900"
     CDS             2132..2488
                     /locus_tag="VLF19_02900"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HSE92226.1"
                     /translation="MRSDDKVLEVLNDLVETCRDGEQGFRTAADAVSDTHIRRLFSTY
                     SEQRGRFATELSAEIERLGGDPERRGSVAGSAHRGWMNIRSAVSGGSTDSVLAEAERG
                     EDAAKAAYERLRDRAA"
     gene            complement(2650..2844)
                     /locus_tag="VLF19_02905"
     CDS             complement(2650..2844)
                     /locus_tag="VLF19_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92227.1"
                     /translation="MLISRCAWHSQYYGRSRWRGVVSWRGWQVKFTDGICARCLTRFF
                     SEHGKSFQRRNTAARRVDAA"
     gene            complement(3159..4316)
                     /locus_tag="VLF19_02910"
     CDS             complement(3159..4316)
                     /locus_tag="VLF19_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSE92228.1"
                     /translation="MRIGLLAPPFERVPPVAYGGTERVVAFLADALVERGHDVTLFAS
                     ADSGTRARLVPTIERPVWHDPRYDDDRPFQVLSAVRAYAHAAALDIMHNHMDYAAWPE
                     ARRAPVPTVTTLHWRTDQPELAPLYAEFKEQRLISISHAQRAPFPDANWVATVHHGYP
                     RDRYRPRLERGRYLAFCGRCAPEKGLDRAIAVALAAGLPIRIAAPRPPENSRHPVVRE
                     QWAYYQRALVPYLHHPLVEYLGELRDGEKQELYENALALLFPIVWPEPFGLVMIEALA
                     CGTPVLATARGSVPEIVRHGETGFLCESVADFVDAVDRVPALDRAVCRRDFERRFTTG
                     VMAAAYETAYERIIAGSSLAPRPLAASPVNGSGGMPAARRMQRIAPIAEPA"
     gene            4565..4783
                     /locus_tag="VLF19_02915"
     CDS             4565..4783
                     /locus_tag="VLF19_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92229.1"
                     /translation="MLRDLGSGNGTYSNGRRVEGSAALNRATRSTRKASLFFDHGPVE
                     PVGEVPPAPRSTSRPSDSTMVRPLDEIH"
     gene            complement(4910..5728)
                     /locus_tag="VLF19_02920"
     CDS             complement(4910..5728)
                     /locus_tag="VLF19_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSE92230.1"
                     /translation="MQGMTGLVGVTAAAAVMLAACATTTEKGVVGVERSQLLLVSSAE
                     MDQSAAAAYQNVLKEEGSKGAVNQNPQQVERIRRIAARLIPQTAVFRKDAPAWKWEVN
                     VITSPEPNAWCMPGGKIAFYTGILDKLQMNDDEVAAVMGHEIAHALREHGRERASQQI
                     ATAVGAGVVGSALGVGKGGTDLVALVGQVTFLLPYSRLHETEADRMGVELAARAGYDP
                     RAAIALWQKMAKLADGGGTPSFLSTHPSNEERIKDLGVFSQKVMPLYEQARAKP"
     gene            complement(5862..6656)
                     /locus_tag="VLF19_02925"
     CDS             complement(5862..6656)
                     /locus_tag="VLF19_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003398011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92231.1"
                     /translation="MLRLNNVQVIYSEVILVLKGLSLEVPEGQIVALLGANGAGKTST
                     LKAVSGLLKTEDGKVTDGSIEFVGRRIDGLDPEQICRLGIVQVMEGRKVLESLTVEEN
                     LRIGGYTRRDPDGVRQDMQMIYGYFPRLVERKDQLAGYLSGGEQQMLVIGRALMAKPR
                     LLLLDEPSLGLAPLLVREIFEIIQKINTEQGTTFLLVEQNAQIALGIAHYGYIMENGR
                     IVFDGPADKLRGNDDVREFYLGLSEAGRRKSFRDVKFYKRRKRWMF"
     gene            complement(6687..7883)
                     /locus_tag="VLF19_02930"
     CDS             complement(6687..7883)
                     /locus_tag="VLF19_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92232.1"
                     /translation="MRSANRPLVRVLLAGLLVAGLGALPAEAQTVIKLGNLVDLTGPT
                     SDQGKDIAQGRIDAVQHFNERGGVNGKKLELVSVEYGFQPPRAVAAYKKFVEGDKVLL
                     VLGYGTPDTEALRPYITKDKVPYISGSYSGHLTDPRQTPYNFPGGIDYTSQMRIFLNY
                     VKETWKDTSRKPRVAFIYADNAYGRAPIESGRLYAKQIGVDLVDEEIIPTVLTDATSQ
                     LLTMKKKDPDFAYINTNTQWVPVVLKDSYKLGIKTKYVVNNYGIDERTPKLAGEAAEG
                     VLGIQDVAYWGENVPGMKTLTEFHQKHHPNDTHASPYMRGWLWVIMAAEAIKRAGDKP
                     TGESVKRALESLKEFDTWGLTPPFTYTSEDHRPTNRARLVMVKGGKIVQVREVSVDRD
                     MKWVGK"
     gene            complement(7932..8975)
                     /locus_tag="VLF19_02935"
     CDS             complement(7932..8975)
                     /locus_tag="VLF19_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92233.1"
                     /translation="MKCGGFRVSYRGDERIFDTAVPIVGLVVLLGVLAVVPRYATTYW
                     LDVLNRIGIAVIGALGLNILVGYTGQISIGHAAFLAVGAYSTAILEADAGLPFYVAIP
                     LGALVAAAVGLIFGIPSLRLKGLYLAIATLAAHFITTYVIIHWESMTKGVLGLMVPPA
                     TFFGVPLDTDARVFYLVYALVVPATLFAKNLFRTKVGRAFIAIRDRDVAASVIGVSLL
                     HYKLLAFVISSFYAGLAGGLMAHHSRILFPDAFTLLVAIDYLAMIIIGGMGSILGSIF
                     GAIFMTLLPEVLKLTATSLTGVYPQAFGFIASGRDIVFGLAVIFFLTYEPQGLARIWV
                     RVRSFWQLWPYAY"
     gene            complement(8972..9859)
                     /locus_tag="VLF19_02940"
     CDS             complement(8972..9859)
                     /locus_tag="VLF19_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92234.1"
                     /translation="MVFMLQLVITGIAVGGVYSLMALGFVLIFKASSVVNFGPGELVL
                     FGAYVAWATILNMRLPLYVALPLTLAIAILMGLVIERGVLRPLIGEPIISVIMVTFGF
                     ASVIRGFLNMTWGSDTRPFPVLFSQQPFHLGPVPVSPVHLWSFVGVLVLLTAFSIFFR
                     RSLTGMAMRATADNQQVALSLGVSVKWIFALSWCIATVVSTLGGIILGSVRGGVDFSL
                     ADLGLKVFPVVILGGLDSVAGAIIGGVLIGVLENLSGGYLDPILGGGVKEVAPFVVLV
                     IILMLRPYGLFGKREIERV"
     gene            complement(9868..11697)
                     /locus_tag="VLF19_02945"
     CDS             complement(9868..11697)
                     /locus_tag="VLF19_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE92235.1"
                     /translation="MPRLLAAQARRFGGRKVALREKKYGIWQEVTWEGYAAHVRAVCL
                     GLLGLGLERGDRVAVISGNRPAWLYTELAAQTAGAIPLGIFVDSLPDQVRLVLQHSGA
                     RFVLVEDQEQADKVLSVRDGVPSLERIIVDDVRGLEGSPDPRLIGLEEVARQGREADA
                     REPGRYDALLERGTPDDVAVLMYTSGTTGVPKAAMISHRNLLATAAGVTRVDPVRETD
                     EIVSFLPFAWVGEQNLSVAIALHSGATVNFPEEPETMRDDLREIGPHVMIAPPRFWET
                     MCSEYQVKIADAGLLKGAATRLALAIGERVAARGPGRRAMGVGPRLLRGVAHLLAFRT
                     MLDKFGLARVRYAYTGGAPLGPEIFGFFRAIGLNLKQVYGQTESSGICVLHPDGDVRA
                     DTLGKPAPGTRVRVSETGEIQVAGDNVFLGYYENPEATARTLSDGWLRTGDAGLVDDR
                     GHLVMIDRLADVLRLADGSRFSPALIENKLKFSPYVREAVVIGENRPHVVALIQIDMG
                     NVGNWAEAGRLPFTTFKDLSRKPEVSALIEEVVARVNQGLPAVARIRAFGLFDKELHA
                     DDGELTRTQKVRRATILGKYRDMIEDLYAREGATADVAGGGWR"
     gene            complement(11721..>11943)
                     /locus_tag="VLF19_02950"
     CDS             complement(11721..>11943)
                     /locus_tag="VLF19_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92236.1"
                     /translation="EDMVRFVLYVKQVKRLTVVMIEHDMGVVMDISDRVAVLDFGRKI
                     AEGTPAEVQRDPVVIKAYLGQDFEVAPVG"
BASE COUNT         1864 a         4173 c         4084 g         1822 t
ORIGIN      
        1 tccagatcac cgctcttctc catcatacga cgaatggccg gggtggggcg ctggctcgcc
       61 gacgcgatgc tctcgacctc ggtgtccacg tcggcgtcgg tcacggccac ggactcccgc
      121 tcggccaccg cctccagcag cagcgcccgg cgcaccccgc gctcggcgcc cgggcgcatc
      181 tcggtgacca gcttgccgta gtcccacggc atggcgtcgg gatcgacgcc ctgccggcgc
      241 agccgctcgc gtgcgtgttc gacctggtgg gcgatgtggc gcatcaccat ggcttccggc
      301 accgcgaatg gatggcgtgc cagcagcgtg tcgatcacct tgtcctcgag ggcatgccgg
      361 tcctcggcgg cgcgccgcgc ctcgagctgg cggagcacgg ccgcccgcaa ggcctccagc
      421 gtgtcgaact cgccgagcga cttggcgagg tcgtcgttca cctcgggcag gaccttctct
      481 ttcacctcgg agaccttgac ggtgccggta ccggacttgc cgcgcagctc ctcgcgtcgg
      541 tgatcgtcgg cgaggcggaa gccgaagctg cgctcttcgc cggcgcgcat gccggcaacg
      601 gcttcgtcca cctcgggcag caccgaaccc tcgccgacga cgaaggcgta ccccatctgt
      661 cgtgtcggct cctgcccgtc gagagtgagc gcgtaatcca cgatcacgag gtcaccgggt
      721 cccgccgcgc gctcgacggc gacgaactgg gcttgcgact cgcgcatgcg ctcgagcgtg
      781 gcgtccacgt cctcggccgt caccgatcct ggcgcgtgct gcacctcgac gctcctgtag
      841 tccccgagct cgatggccgg cttcacctcg accacggcga cgaacgacag cggcgcgccc
      901 tcctcgagtc gcacctcctg aaggtcggga tccgccaccg gctccagacg cacctcggtc
      961 agggcccggc gatagacgtc ggggatgagg tgctccgcga cctcccggcg cacgtcgtcg
     1021 gcgaagtgca ccttcacgag ggagcgcggg acgtggccgc ggcgaaaccc aggcaggcgc
     1081 gcatgcttct gcacccgacc gtacgcccgc tcccattcct tctgcacgac gtccgccggc
     1141 gcctcgacgg ccacccgacg cttgcagcct tccatttcct cgacggcgac cttcatcgct
     1201 cttcacgctc cgcggcggtc atgatatggc ccccaagccc tcaacgttcg gttcgccccg
     1261 gccagccagg gcgggcctcc ttgacccgac tcgctcccgg agacatagag cgcagccccc
     1321 caacgttcgg cccgccccgg cgcgatcgga gcgcgccagt taccacggat cgttccttgg
     1381 atggtgcgag aggggggagt cgaaccccca agggcgttta acccaccgga tcctaagtcc
     1441 ggcgcgtctg ccagtttcgc cactctcgcg cgacgtgggg cacgcctcga gcagcatacc
     1501 cacgaccgca gaaacccgca aggcgataga ccttttgtag aagtgtgcga cagcgctcgg
     1561 cacggccgcg gcctcggtcg cggtgtagag cctggcgtgg gcgggcaacg ccacccaggc
     1621 ggacttcgac gcgtgcaacc gggaggcgca ggctcgcgct cttgagcccc gcggactcgc
     1681 cgagcggctc gcgtaccggt agcaccacgc cgggcggagg cgtctcgagc ggcagcagca
     1741 ccggcagcac gatgtccggc accacgtcgg gctcgggcag cggcgtctcg ggcggcaccg
     1801 ggtcctccga ttccacgtca gctaccggca gcggcgcccc cgacggtagc ggcgtttcca
     1861 gcaccacgtc gggcgggagc gtctgcggcg gcaccgccgc cagtggatcc gattccggcg
     1921 gcgcgtccgg cacgggcgcc agcggcgcgc gtcgaccgac gccgcgctgc acggcatcgc
     1981 gggggccagc gcgggcgacc cagcctacca gcaagcgtac cgggattgca tgaagaggcg
     2041 agggttctag atcggcggct gtacgagggg ctcactgcgg gagcccctcg tcatgcctac
     2101 caaagagttc gtcgacataa aggaggacgt gatgaggtcc gatgacaagg tcctcgaagt
     2161 cctgaacgat ctcgtcgaga cgtgcaggga cggcgagcaa ggatttcgca ccgccgccga
     2221 cgcggtcagc gacactcaca tcaggcggct tttcagcacc tactccgagc agcgcgggcg
     2281 gttcgcgacc gagctgtctg ccgagatcga gcgtctgggc ggcgaccccg agcgccgcgg
     2341 cagcgtcgcg ggttcagcgc accggggatg gatgaacata cgatccgccg tgagcggcgg
     2401 gagcacggac tcggtgctcg ccgaggccga gcgcggggag gacgctgcga aggcggcgta
     2461 cgagcggctc cgcgaccggg cggcctagcc caggaacatc gccggccatc gagcgtggcc
     2521 ccaattcgcc gggatccgcg ccccatcgct ggaggctcgg gtccatccag cgcacatcga
     2581 ggcgcgccac cgccttgcct ggggccggcc tgaacgcttg gggctagggg gggcctaata
     2641 tgtcattcgt caggcggcgt cgacgcgtcg ggcggcagtg tttcgacgct ggaagctctt
     2701 gccgtgctcc gaaaagaagc gcgtgaggca gcgggcgcag atgccgtcgg tgaacttgac
     2761 ctgccatccg cgccaggaga cgacaccacg ccagcgggaa cggccgtagt attgcgagtg
     2821 ccaggcgcaa cgggagatca gcatcgaggg ctcctgccgg ccttgtccac gaaggtaccg
     2881 cgctgcaagg catatgcccc gccgcgggcg ctgtaatgcc gagcttttga cgctcactgc
     2941 gcaacgcagg gcgggaattc tcggcccacc gagggaaaaa ttcttcccac cgctggcgaa
     3001 cccgcgccgg ctgcagaggg cgatggcgcg gcgtccgtag ccgcagccac cacacgctca
     3061 agcccggcgg ctgggacggc cgatctgggc cgtctggcgt gggcaacggc gggcgcattc
     3121 cgctgtcggt cgaccggatg tcccgagggt gagggcctct acgcgggctc ggcgatcggc
     3181 gcgatccgct gcattcgccg ggccgccggc atgccgccac tgccgttcac gggggacgcc
     3241 gccagcggcc ggggggcgag cgacgatccg gcgatgatcc gctcgtaggc ggtctcgtag
     3301 gcggccgcca tcacgcccgt ggtgaaccgt cgctcgaagt cgcggcggca cacagcgcgg
     3361 tccagcgccg gcacccggtc gacagcgtcg acgaagtctg ccacggactc gcagaggaac
     3421 ccggtctcgc cgtgacggac gatctcgggt acggacccgc gagccgtggc cagcacgggc
     3481 gtgccgcagg cgagcgcttc gatcatcacg agaccgaagg gctccggcca cacgatcggg
     3541 aagagcagcg ccagcgcgtt ctcgtaaagc tcctgcttct cgccgtcacg caactcgccg
     3601 agatactcga cgagaggatg gtggaggtac ggcacgagcg ccctctggta gtaggcccac
     3661 tgctcgcgaa ccacggggtg ccgcgagttc tcgggtggcc gcggggccgc gatccggatc
     3721 ggcaagccgg cggcgagcgc cacggcgatg gcccggtcga gtcccttctc cggcgcgcag
     3781 cggccgcaga acgccaggta tcgtccacgc tctagccgcg gcctgtacct gtcgcgcggg
     3841 tagccgtgat gcaccgtggc cacccagttc gcgtccggga acggcgcgcg ctgcgcgtgg
     3901 ctgatggaga tcagccgctg ttccttgaac tcggcataga gcggggccag ctccggctga
     3961 tcggtgcgcc agtgcagcgt cgtcaccgtc ggcaccggcg cgcgtcgggc ctcgggccat
     4021 gcggcgtagt ccatgtggtt gtgcatgatg tcgagggccg cggcgtgcgc gtaggcgcgc
     4081 acggcggaca gcacctggaa cggtcggtcg tcgtcgtacc gggggtcgtg ccagacggga
     4141 cgctcgatgg tcggcaccag ccgggctcgc gtccccgagt cggcgctggc gaagagggtg
     4201 acgtcgtgac cgcgctcgac gagtgcgtcg gcgaggaacg cgaccacacg ctcggtacca
     4261 ccgtacgcga ccggaggcac ccgctcgaac ggtggagcga gcaagccgat tctcacggca
     4321 ggcaatagtg cagtcgccat gccggcgacc ctgcgcatcg gcaaggccgc ggccagcgtc
     4381 gtcggacccg agccgcaacg taccctgccg cggcccgtgc cccgtccggc ggcaacgccg
     4441 gagagcgacg aatgctggca cctccgggtc agcgtgacat gggacgacct ggagtgtttt
     4501 tccgcggagc ccgggcgaat cgccgtttct tcaatcgctt agggcctggc gttacgcctg
     4561 gtgggtgtta cgcgacctcg gctcgggcaa cgggacgtac tccaatggac ggcgggtgga
     4621 gggcagcgcg gcactgaacc gggcgacgag atcgactcgg aaggcctcgc tgttcttcga
     4681 ccacgggccg gtcgagccgg tcggcgaggt gccgccggcg ccacggtcca cgtcgaggcc
     4741 gtccgacagc acgatggtcc ggcccctcga cgagatccat tgaagacggt cgtcagtgac
     4801 cgctacgagg tgctggtcga gctgggtcgg ggcaccacgg cggtcgtcta caaggcgcgc
     4861 gactcgtacc tcgaccgtac ggtcgccatc aaccccgcgt gggccgaggc tacggcttcg
     4921 cccgcgcctg ctcgtagagc ggcatcacct tctgcgagaa gaccccgagg tccttgatgc
     4981 gctcctcgtt cgaggggtgg gtcgagagga acgacggcgt tccgcctccg tccgcgagct
     5041 tggccatctt ctgccagagc gcgatggccg cccgcggatc gtagcccgct cgcgccgcca
     5101 gctcgacgcc catgcggtcc gcttcggtct cgtggagccg cgagtacggg agcaggaacg
     5161 tgacctgtcc gaccagggcc acgaggtcgg tccccccctt cccgaccccg agcgccgagc
     5221 cgaccactcc cgcgccgacg gccgtcgcga tctgctgcga tgcccgctcg cgcccgtgct
     5281 cgcgcagcgc gtgcgcgatt tcgtggccca tgacggcggc gacctcgtcg tcgttcatct
     5341 ggagcttgtc gaggatgccg gtgtagaacg cgatcttgcc gccgggcatg caccaggcgt
     5401 tgggctccgg cgaggtgatc acgttcacct cccacttcca ggccggcgcg tccttccgga
     5461 acaccgcggt ctgggggatg aggcgcgcag cgatccggcg gatgcgctcg acctgctgcg
     5521 gattctggtt gacggcgccc ttgctgccct cctccttcag cacgttctgg taggcggcgg
     5581 ccgcgctctg atccatctcc gccgaggaca cgagcaggag ctggctgcgc tcgacgccga
     5641 cgacgccctt ctcggtcgtc gtcgcgcacg cggcgagcat caccgcggcc gccgcggtca
     5701 ctccgacgag gccggtcatc ccctgcatca atgggccctc cttcgacggt cggcgcccgg
     5761 cgaccactcg cgcctccgtg cggtatcgta ctcccgccgg caaggtggga aggcctgccg
     5821 catccgctga gtgatggttg cgggccggcg tgaggggcgg ctcagaacat ccagcgcttg
     5881 cgacgcttgt agaacttgac gtcgcggaag ctcttgcgcc tccccgcctc gctcaggccg
     5941 aggtagaact cgcggacgtc gtcgttgccc cggagcttgt cggccggacc gtcgaagacg
     6001 atccggccgt tctccatgat gtagccgtag tgggcgatgc cgagcgcgat ctgggcgttc
     6061 tgctcgacga gtaggaaggt ggttccttgc tcggtgttga tcttctggat gatctcgaag
     6121 atctcccgga ccagcagcgg cgcgagcccg agcgagggct cgtcgagcag cagcagccgg
     6181 ggcttggcca tcagcgcgcg cccgatcacc agcatctgct gctcgccgcc gctcagatac
     6241 cccgccagct ggtccttgcg ttccaccagg cgggggaagt agccgtagat catctgcatg
     6301 tcctgccgga cgccgtcggg atcgcggcgc gtgtagcccc cgatcctcag attctcctcg
     6361 acggtgagac tctcgaggac cttccgcccc tccatcacct gcacgatgcc cagccggcag
     6421 atctgctcgg gatcgaggcc gtcgatccgc cggccgacga actcgatgct gccgtcggtc
     6481 accttgccgt cctcggtctt caagaggccg gacacggcct tgagcgtcga ggtcttgccc
     6541 gcgccgttgg cgcccagcaa ggccacgatc tggccctcgg ggacctccag cgagaggccc
     6601 ttgaggacca ggatcacctc gctgtagatg acctggacgt tgttcagccg cagcatcgag
     6661 ccgccccgat cacccggcga cgacggtcac ttgccgaccc acttcatgtc gcgatcgacg
     6721 gagacctcgc gcacctgcac gatcttgccg cccttgacca tgaccagccg cgcccggttg
     6781 gtcggccgat ggtcctcgct ggtgtaggtg aacggcgggg tgagccccca cgtgtcgaac
     6841 tccttcaggc tctcgagcgc tcgcttgacg gactcgcccg tcggcttgtc gcccgcgcgc
     6901 ttgatggcct cggcggccat gatcacccac agccaccccc gcatgtacgg cgaggcgtgc
     6961 gtgtcgttgg ggtggtgctt ctggtggaac tccgtcagcg tcttcatgcc ggggacgttc
     7021 tcgccccagt aggcgacgtc ctggatgccc agcacgccct cggccgcctc ccccgccagc
     7081 ttgggcgtgc gctcgtcgat cccgtagttg ttgaccacgt acttcgtctt gatgccgagc
     7141 ttgtaggaat ctttcagcac cacgggcacc cactgcgtgt tggtgttgat gtacgcgaag
     7201 tccgggtctt tcttcttcat ggtcaggagc tgcgaggtgg cgtcggtgag gacggtgggg
     7261 atgatctcct cgtccacgag gtccacgccg atctgcttcg cgtacaggcg tccggactcg
     7321 atcggggccc gcccgtacgc gttgtcggcg tagatgaacg ccacgcgcgg cttgcggctc
     7381 gtgtccttcc aggtctcctt cacgtagttc aggaagatgc gcatctggct cgtgtaatcg
     7441 atgccgccgg ggaagttgta cggggtctgg cgcggatcgg tgaggtggcc ggagtacgac
     7501 ccggagatgt agggcacctt gtccttcgtg atgtacggac gcagggcctc ggtgtcgggc
     7561 gtgccgtagc cgagcacgag gaggaccttg tcgccctcca cgaacttctt gtacgcggcc
     7621 accgcccgcg gcggctggaa gccgtactcc acgctgacca gctcgagctt cttgccgttg
     7681 acgccgccgc gctcgttgaa gtgctggacg gcgtcgatgc ggccctgggc gatgtccttg
     7741 ccctggtcgg acgtggggcc ggtgaggtcg acgaggttgc ccagcttgat gacggtctgg
     7801 gcttcggccg gcagagcacc caggccggcg acgagcaggc cggcgaggag gacccgaacg
     7861 agcggacgat tcgcgctcct catgacgctc tccttctcac gagttcgacg gtgttcttcg
     7921 ccgcgcatcg gtcagtacgc gtacggccac agctgccaga agctgcggac ccgcacccag
     7981 atgcgcgcga gcccttgtgg ctcgtacgtc aggaagaaga tgacggccag gccgaagacg
     8041 atgtcgcgcc ccgaggcgat gaagccgaac gcctgcgggt agactccggt gagcgacgtc
     8101 gccgtgagct tcagcacctc gggcagcagg gtcatgaaga tcgccccgaa gatggagccg
     8161 aggatgctgc ccatgccgcc gatgatgatc atcgccaggt agtcgatggc cacgagcagg
     8221 gtgaacgcgt cggggaacag gatcctcgag tggtgggcca tcaacccgcc cgcgagaccg
     8281 gcgtagaacg agctgatcac gaaggcgagg agcttgtagt ggaggaggct caccccgatc
     8341 acgctggcgg ccacgtcgcg gtcccggatc gcgatgaagg cccggccgac cttggtgcgg
     8401 aagagattct tggcgaagag cgtcgccggc accaccagcg cgtacacgag atagaagacg
     8461 cgggcgtccg tgtccagcgg caccccgaag aacgtcgccg gcgggaccat gagcccgagc
     8521 acgcccttgg tcatgctctc ccagtggatg atcacgtacg tggtgatgaa gtgcgcggcc
     8581 agggtggcga tggcgagata gaggcccttg aggcgcagcg acgggatgcc gaagatcagg
     8641 ccgacggccg cggccacgag ggcgcccagc gggatcgcca cgtagaacgg cagccccgcg
     8701 tccgcctcga ggatcgcggt ggagtaggcg cccacggcca ggaaggcggc gtgcccgatg
     8761 gagatctgtc ccgtgtagcc gacgaggatg ttcaggccca gcgccccgat gacggcgatg
     8821 ccgatccggt tgaggacgtc gagccagtac gtcgtggcgt accgcgggac cacggcgagc
     8881 acgccgagga ggaccacgag cccgacgatc ggcaccgcgg tgtcgaagat ccgctcgtcg
     8941 ccccggtagc tgacgcgaaa cccgccgcac ttcatacgcg ctcgatctcc cgcttgccga
     9001 agagcccgta ggggcgcagc atgaggatga tgacgaggac cacgaagggc gccacctcct
     9061 tgaccccgcc gccgaggatc gggtccagat agccgcccga gaggttctcc agcaccccga
     9121 tcagcacgcc gccgatgatg gccccggcga cgctgtcgag cccgccgagg atgacgaccg
     9181 ggaacacctt caggccgagg tcggccagcg agaagtccac cccgccgcgc acgctgccca
     9241 ggatgatccc gcccagggtc gagaccacgg tggcgatgca ccacgagagc gcgaagatcc
     9301 acttcacgct cacgcccagc gacagcgcga cctgctggtt gtccgcggtc gcgcgcatcg
     9361 ccatgcccgt gagcgagcgc cggaagaaga tcgagaacgc ggtcaacaag acgagcacgc
     9421 cgacgaagct ccagaggtgc accggcgaca ccggcaccgg gcccaggtgg aagggctgct
     9481 gggagaacag gaccgggaac gggcgcgtgt cgctccccca ggtcatgttg aggaatcctc
     9541 gaatcacgct ggcgaagcca aaggtgacca tgatcacgga gatgatcggc tcgccgatca
     9601 gcggccgcag cacaccccgc tcgatgacga gccccatcag gatcgcgatg gcgagggtca
     9661 gcggcaaggc cacgtacagc ggcagcctca tgttgaggat cgtcgcccac gccacgtacg
     9721 cgccgaagag caccagctcg cccggcccga aattgaccac gctcgaggcc ttgaagatga
     9781 ggacgaaccc gagcgccatg agcgagtaga cgccgccgac ggcgatgccg gtgatgacga
     9841 gctggagcat gaagacgatc tgctcgctca tcgccatcct ccgcccgcga cgtccgccgt
     9901 ggcgccctcg cgcgcgtaca ggtcctcgat catgtcccgg tacttcccga ggatcgtcgc
     9961 gcggcggacc ttctgcgtcc gcgtcagctc gccgtcgtcc gcgtgcagct ccttgtcgaa
    10021 gagcccgaac gcgcggatgc gggcgacggc ggggaggccc tggttgacgc gcgccaccac
    10081 ctcctcgatc agcgcggaca cctcgggctt gcgcgagagg tccttgaagg tggtgaaggg
    10141 cagccggccg gcttcggccc agttccccac gttgcccatg tcgatctgga tcagggccac
    10201 gacgtgcggc cggttctcgc cgatcaccac cgcctcgcgg acgtacgggc tgaatttcag
    10261 cttgttctcg atcagcgcgg gagagaagcg cgagccgtcg gccagccgga ggacgtccgc
    10321 gagccggtcg atcatgacca ggtggccccg gtcatcgacg aggcccgcat cccccgtacg
    10381 cagccagccg tcactgagcg tcctcgcggt ggcttcgggg ttctcgtaat agccgaggaa
    10441 cacgttatcg ccggcgacct ggatctcgcc ggtctccgag acgcgcaccc gcgtgcccgg
    10501 cgcgggcttg ccgagcgtgt ccgcccggac gtctccgtcc gggtgcagca cgcagatccc
    10561 cgacgattcc gtctggccgt acacctgctt gaggttgagc ccgatcgcgc ggaagaaccc
    10621 gaagatctcc gggcccagcg gggcgccccc cgtgtacgca tagcggacgc gcgcgaggcc
    10681 gaacttgtcg agcatggtcc ggaacgccag caggtgcgcg accccgcgca ggaggcgcgg
    10741 cccgaccccc atcgcccggc ggcccgggcc ccgcgcggcc accctctcgc cgatcgccag
    10801 ggcgagccgc gtcgccgcgc ccttgaggag cccggcgtcg gcgatcttca cctggtactc
    10861 cgagcacatc gtctcccaga agcgcggcgg cgcgatcatc acgtggggcc cgatctcccg
    10921 caggtcgtcg cgcatcgtct cgggctcctc ggggaagttc acggtggccc ccgagtgcag
    10981 ggcgatcgcg acgctgaggt tctgctcgcc gacccacgcg aaggggagga aggagacgat
    11041 ctcgtccgtc tcgcggacgg gatccacccg cgtgacgccc gcggccgtcg cgaggagatt
    11101 gcgatggctg atcatcgccg ccttgggcac gcccgtcgtc cccgaggtgt acatcagcac
    11161 cgccacgtcg tcgggtgtcc cgcgctcgag cagcgcgtcg tagcggcccg gctcgcgggc
    11221 gtcggcctcg cgcccctgcc gcgccacctc ctccaggccg atcagccgcg ggtcagggct
    11281 cccctccagc ccacgcacgt cgtcgacgat gatccgctcg aggctcggaa cgccatcgcg
    11341 cacgctcagg accttgtcgg cctgctcctg gtcctcgacc aggacgaagc gcgccccgct
    11401 gtgttgcagg acgagccgta cctgatccgg gaggctgtcg acgaagattc ccagcgggat
    11461 ggcgccggcc gtctgcgccg cgagctcggt gtagagccag gccggccggt ttccggagat
    11521 caccgccacc cggtcgcccc gctcgaggcc gagcccgagc agacccaggc acacggcgcg
    11581 cacgtgcgcg gcgtagccct cccaggtcac ctcctgccag atcccgtact tcttctcgcg
    11641 cagggcgacc ttgcggccgc cgaagcgccg ggcctgggcg gcgagcagcc gcggcaacgt
    11701 ctctcgatcg agggccggcg ctaccccacg ggcgccacct cgaagtcctg acccaggtag
    11761 gccttgatca ccacgggatc ccgctggacc tcggccggcg tgccctcggc gatcttccgc
    11821 ccgaaatcga ggacggccac ccggtcggag atgtccatga ccacgcccat gtcgtgctcg
    11881 atcatcacca cggtcaggcg cttcacctgc ttgacataga ggacgaagcg caccatgtcc
    11941 tcg
//
LOCUS       DATIAZ010000086         3949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_152421, whole genome shotgun sequence.
ACCESSION   DATIAZ010000086 DATIAZ010000000
VERSION     DATIAZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152421"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1074)
                     /gene="hpnA"
                     /locus_tag="VLF19_02955"
     CDS             complement(58..1074)
                     /gene="hpnA"
                     /locus_tag="VLF19_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HSE92237.1"
                     /translation="MDALVTGGTGFVGANVVRELLRAGATVRVLARRGGERRALAGLA
                     VEIAEGDLRDPAAVRRAVAGVATVFHVAADYRLWAPDPRVLYATNVDGTRGLLESAAA
                     AGVDRIVYTSTVGALGLPGNGAPGTEDTPVRLGDMVGPYKRSKFLAEQVALEFARAGA
                     PVVVVNPSAPIGPWDVKPTPTGQMIVDFLQGRMFGSLDTGLNVVHVRDVARGHLLAAE
                     RGRPGQKYILGHANLALTEIFTLLAGITGRRAPRLRVPYAVAWLAAAGMEGAARLTGR
                     PPRVPLTAVRMARKRMFFSPAKAVRELGLPQTDVREALADAVTWFAEHGYARVVTRPG
                     AAAQ"
     gene            complement(1236..1910)
                     /locus_tag="VLF19_02960"
     CDS             complement(1236..1910)
                     /locus_tag="VLF19_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92238.1"
                     /translation="MTSVLVLTAVDVEARGLARHLGLARVPDRDWPCYRGGRIEIACI
                     GPRAVRLSERLTDDWLAPAVVVSAGVCGALAPALATGALVVPEAVVVDGARLALDPLP
                     GLPASGTLLSVASVVGTPTAKARLWMETGAIAMDMESGAIAAWTRRRGTRVAVVRGVS
                     DTAQETVPADLAALVEAGGRVRAASALRAVLARPRAARDALALGRGTAAALKSVAAAL
                     ASLART"
     gene            complement(1907..2236)
                     /locus_tag="VLF19_02965"
     CDS             complement(1907..2236)
                     /locus_tag="VLF19_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92239.1"
                     /translation="MSDERTLEGWAPEIGEGATLDEVVDLAFDYRGDVTVVTADGREI
                     VGFVYSRERDVAEPYLKMFEPAGASLTLRYTEVRVIRFSGKDAAAGRSYEAWLRRKAQ
                     GAPPPGP"
     gene            complement(2233..3255)
                     /gene="hpnH"
                     /locus_tag="VLF19_02970"
     CDS             complement(2233..3255)
                     /gene="hpnH"
                     /locus_tag="VLF19_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HSE92240.1"
                     /translation="MSVPLSQMWTVATYVLGQRLRGRRRYPLVLMLEPLFRCNLACAG
                     CGKIQYPAHILKRHLTVEQCLAAVEECGAPMVSIPGGEPLMYPDIGRLVQALVGRRKY
                     VYLCTNAILLKEKLAEGLFEPSKYLSFSVHMDGLREDHDEAVCREGVYDEAVEAIREA
                     IGRGFRVTTNSTLFEGADPRRMRKFFDAMMALGVEGMMISPGYSYSKAPDQEHFLRRR
                     RAHELFSAMLSSPNKRWRFNQSPLFLQFLMGKQDFECTPWGNPTYNLFGWQRPCYLLQ
                     EGYAATFQELMETTRWERYGHRSGNPKCRDCMVHCGYEPTAVNHTFASWRGFRDAVAA
                     TVTGRL"
     gene            complement(3257..>3949)
                     /locus_tag="VLF19_02975"
     CDS             complement(3257..>3949)
                     /locus_tag="VLF19_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HSE92241.1"
                     /translation="ADVGARRALGFAGVVWPLAWLAEFASTRWGLPFGLYHYTGATRG
                     RELYVANVPFMDSLSFTFLAYAAFCLARWAWRGRQPSRAALAATAGGLMMLLDVVIDP
                     VAVRGDRWFLGRIFYYAEPGAYFGVPLSNFAGWWLVGALGVGLWLRGSGWSATDGGQP
                     AFGVGLYYAVLGFNLAVTAWLEEWLLLASGVTLHATAALAVFGRRPFRDPAWPAQLPG
                     GGIGRGAPPPTE"
BASE COUNT          595 a         1459 c         1420 g          475 t
ORIGIN      
        1 catagtagaa gttcgaccgg ctgcgccgcg tcagccgcga gacgaggtcg gcggggctca
       61 ctgcgcggcc gcccccggcc gcgtgacgac acgcgcatac ccgtgctcgg cgaaccaggt
      121 gacggcgtcg gccagcgcct cgcgcacgtc cgtctgcggc agccccagct cccggaccgc
      181 cttcgccggg ctgaagaaca tgcgcttcct cgccatgcgc acggccgtga gcggcacgcg
      241 gggcggccgg cccgtgagcc gcgccgcgcc ctccatgccg gcagcggcga gccaggccac
      301 cgcatagggc acgcgcaggc ggggcgcccg ccgcccggtg atcccggcca ggagcgtgaa
      361 gatctccgtc agcgcgaggt tcgcgtggcc gaggatgtac ttctgtccgg gccgcccgcg
      421 ctccgcggcg agcaggtggc cgcgcgcgac gtcgcggacg tggacgacgt tgagcccggt
      481 gtcgagcgag ccgaacatgc gtccctggag gaaatcgacg atcatctgcc cggtcggcgt
      541 gggcttgacg tcccacgggc cgatcggcgc cgagggattc acgacgacga cgggcgcgcc
      601 agcgcgcgcg aactcgagcg ccacctgctc ggcgaggaac ttcgagcgct tgtaagggcc
      661 gaccatgtcg ccgagcctca ccggcgtatc ctcggtcccc ggcgcgccgt tcccgggcag
      721 tccgagggca ccgacggtgg aggtgtacac gatccggtcg acccccgccg cggcggcgga
      781 ctccaggagg ccgcgcgtgc cgtccacgtt ggtggcgtag aggacgcgtg ggtctggcgc
      841 ccacagccgg tagtcggcgg ccacgtggaa cacggtggcg acaccggcca ccgcgcggcg
      901 caccgccgcg ggatcgcgca ggtcgccctc tgcaatctcc acggccaggc cggccagcgc
      961 ccggcgctcg ccaccccggc gcgccagcac ccgcaccgtc gcgccggcgc gcagcaactc
     1021 gcggacgacg ttggcgccca cgaacccggt gccgcccgtc accagcgcgt ccatctatcg
     1081 tggagaccgc cagcccgggt tgagcctcgc atgacccacc gccgatgcac gaggctcccc
     1141 tcgcccggcc agcggccggt aacgatcatc gcggggtttc acgcgttcga acggcggcag
     1201 atctcggatg gtgcccggct ccgcgtcccc ggcgctcatg tcctggccag cgacgcgagg
     1261 gcggcggcga cggatttcag tgcggcggcg gtgccgcgac cgagcgcgag ggcatccctc
     1321 gcggcgcggg gcctggcgag gacggcgcgc agggcagagg ccgcccgcac ccgccccccg
     1381 gcctcgacga gcgccgcgag gtcggcgggc acggtctctt gcgcggtgtc cgagacgccg
     1441 cgcacgacgg ccacgcgcgt gccgcggcga cgcgtccagg ccgcgatcgc gccggactcc
     1501 atgtccatcg cgatcgcgcc cgtctccatc cagagccgtg ccttggccgt cggcgtgccg
     1561 accacggacg ccacgctcag cagggtccct gaagccggca gccccgggag cgggtcgagc
     1621 gccagccgcg ccccgtccac gacgacggcc tcgggcacga cgagcgcgcc ggtggccagc
     1681 gccggcgcga gcgcgccgca gacaccggcg gagacgacca cggccggcgc cagccagtcg
     1741 tcggtcagtc gctcggacag ccgcaccgcc cgcggcccga tgcacgcgat ctcgatgcgg
     1801 ccgccgcggt agcacggcca gtcgcggtcc gggacgcgcg cgaggcccag atggcgggcc
     1861 agcccacgcg cctcgacgtc gacggcggtg agcacgagga cggaggtcat gggcccggcg
     1921 gcggggcgcc ctgggccttg cggcgtagcc aggcctcgta ggatctgccg gccgcggcgt
     1981 ccttgcccga gaagcggatc acccggacct cggtgtagcg aagggtgagc gaggcgcccg
     2041 cgggctcgaa catcttcaga tacggctccg cgacgtcgcg ctcgcggctg tacacgaagc
     2101 cgacgatctc ccggccgtcg gcggtcacca cggtcacgtc accgcgatag tcgaaggcca
     2161 ggtcgacgac ctcgtccagg gtcgcgccct cgccgatctc gggggcccag ccttcgagcg
     2221 tccgctcgtc gctcacaggc ggccggtcac cgtcgccgcg acggcgtcgc ggaacccgcg
     2281 ccaggaggcg aacgtgtggt tcaccgcggt cggctcgtac ccgcagtgga ccatgcagtc
     2341 gcggcacttg ggattgccgc tccggtgccc gtagcgctcc cagcgcgtgg tctccatcag
     2401 ctcctggaag gtcgccgcgt agccctcctg gagcaggtag cagggccgct gccacccgaa
     2461 gaggttgtag gtcgggttgc cccacggcgt gcactcgaag tcctgcttgc ccatgaggaa
     2521 ctgcaggaac agcggcgact ggttgaaccg ccagcgcttg ttcggactcg agagcatggc
     2581 ggagaacagc tcgtgggcgc gccggcgccg caagaagtgc tcctggtccg gcgccttgga
     2641 gtacgagtag ccgggcgaga tcatcatgcc ctcgacgccg agggccatca tggcgtcgaa
     2701 gaatttccgc atgcgccggg gatcggcccc ctcgaagagc gtgctgttgg tggtgacgcg
     2761 gaagccgcgg ccgatggcct cgcgaatggc ctcgaccgcc tcgtcgtaca caccctctcg
     2821 gcacacggcc tcgtcgtggt cttcccgcag gccgtccatg tggacgctga acgagaggta
     2881 cttggacggc tcgaagaggc cctccgcgag cttctccttc aggaggatcg cgttggtgca
     2941 gaggtacacg tatttccgcc ggccgaccag cgcctgcacc agccggccga tgtccgggta
     3001 catcagcggc tcgccgccgg ggatcgagac catcggggcg ccgcactcct ccaccgccgc
     3061 cagacactgc tcgacggtca ggtgccgctt gaggatgtgg gcggggtact ggatcttccc
     3121 gcacccggcg caggccaggt tgcagcggaa gagcggctcc agcatgagca cgagcgggta
     3181 gcggcgccgg ccgcggaggc gctgcccgag cacgtaggtc gcgaccgtcc acatctgcga
     3241 gagcggaacg ctcatatcac tcggtggggg ggggcgcccc ccttccgata cctccccccg
     3301 ggagctgcgc cggccaggcc ggatctcgaa agggtctgcg cccgaacacg gccagcgccg
     3361 ccgtcgcgtg caatgtaacg ccggaagcca gcaggagcca ctcctcgagc caggccgtga
     3421 ccgccaggtt gaaaccgagc acggcgtagt atagcccgac accgaaggcg ggctgcccac
     3481 catcggtggc ggaccacccg ctgccccgta gccagagacc gacgccgagc gcgccgacca
     3541 gccaccaccc ggcgaagttc gacagcggca ccccgaagta cgcgcccggc tccgcgtagt
     3601 agaagatccg gcccaggaac cagcggtcac cgcggaccgc caccggatcg atgacgacgt
     3661 cgagcagcat catgaggccg ccggcggtcg ccgcgagcgc cgcgcgtgac ggctggcggc
     3721 cgcgccacgc ccagcgggcg agacagaacg ccgcgtacgc caggaacgtg aaggacagcg
     3781 agtccatgaa cgggacgttc gcgacgtaga gctcgcggcc gcgcgtggcg cccgtgtagt
     3841 ggtagaggcc gaagggcagg ccccagcgcg tcgacgcgaa ctcggcgagc cacgccagcg
     3901 gccacacgac gccggcgaag ccgagcgccc ggcgcgcgcc cacgtcggc
//
LOCUS       DATIAZ010000087         4636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_284337, whole genome shotgun sequence.
ACCESSION   DATIAZ010000087 DATIAZ010000000
VERSION     DATIAZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284337"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VLF19_02980"
     CDS             complement(<1..1176)
                     /locus_tag="VLF19_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE92242.1"
                     /translation="MHENGHQDARTLHARLNHPVIDADGHWLEYAPVMREEFRRIGGD
                     AAAEALEVASQRVPNALRMSLAERTQRRVGQEAFWGSPCRNVLDRATAMLPRLMYERL
                     DDLGIDLCVVYPTAGLSYHRMQDTRLRRAICRAYNVFTADQFRGLEDRVIPAAIIPMY
                     TPDEAVEELDFAAKQLGYKVMMVGGLMRRPVPALAAEHPDTSRFVEWYDVVGIDSPHD
                     YDPVWEKCRELRVAPSFHNGARSILLRNSPSNFCYNHIGHFASAGHAVAKALFFGGVT
                     RRFPDLNFAFLEGGVGWACMLYADLIGHWEKRNRQALESTNPGNLDRGALLALAQRYG
                     REAVVEAVGRGDGLEGDSNSVLTGGIEDLDDYFRCKIERKEDIHELFVPRYYFGCEAD
                     "
     gene            complement(1277..2470)
                     /locus_tag="VLF19_02985"
     CDS             complement(1277..2470)
                     /locus_tag="VLF19_02985"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92243.1"
                     /translation="MSRSGFADVTHDEALRRARALVPVLRERADGTEAARQMPKETLE
                     DLHRAGLLRFHQPRRWGGMELPFVAVLDIPAEIARGCASTAWNIGNLGIHHWMLALYD
                     ERAQEEVWGRNPDALIASGIAYPQGRGRKVDGGFVVSGLWNFSSGVDACDWNMLAVTV
                     REGDRVVDHRMCLVPRTEYEIVDDWHVLGMQGTGSKSVRATEIFVPEHRALCMYLARG
                     GAEFPGARVNPNPLYRVPLSGLGSHCLAGTGVGNAQAALELTIEAIRERSTSYTAMRM
                     RDFQAVQLRVGRAGAMIDAARLVIREDCLDAERIAREDRVPSVEDKLRFKRNVAYAMQ
                     LCTEAVDSLHALAGANGIYDRYPIQRLFRDQHALSAHIGFSWDAQGGPWALVALGGEF
                     SSPTL"
     gene            complement(2467..2934)
                     /locus_tag="VLF19_02990"
     CDS             complement(2467..2934)
                     /locus_tag="VLF19_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE92244.1"
                     /translation="MTELNHYFVRANDLEKTREFYCDVLGFQVMPRPTFPFPGYWLGV
                     NGKIQVHMGPDGIDNADLYYLGTPKGAVKDHAGVVDHIAFVATEPGGFIRRFKERGID
                     YRPRSLPEFDLYQIFITDPNGLTIELNFFGLKDVTDWGGEDYSKMPQVAGMRA"
     gene            3255..4040
                     /locus_tag="VLF19_02995"
     CDS             3255..4040
                     /locus_tag="VLF19_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92245.1"
                     /translation="MALLALEGLHLNFGGVAALAGVSLEVAAGEFFAVIGPNGAGKTS
                     IFNCISGIYAPTRGRIRFDGRDITPLPPHQRARLRIARTFQNIALFKGMSVLDNVKIG
                     RHVHLRSGPLACGLYYGRAAREERAHRATIEREIIELLELQDIRHKIVGTLPYGLQKR
                     VELARALALDPVVLLLDEPTAGMNAEETEDMVRFVLYVKRVKRLTVVMIEHDMGVVMD
                     ISDRVAVLDFGRKIAEGTPAEVQRDPVVIKAYLGQDFEVAPVG"
     gene            4064..>4636
                     /locus_tag="VLF19_03000"
     CDS             4064..>4636
                     /locus_tag="VLF19_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE92246.1"
                     /translation="MPRLLAAQARRLGGRKVALREKKYGIWQEVTWEGYAAHVRAVCM
                     GLLGLGLERGDRVAVISGNRPAWLYTELAAQTAGAIPLGIFVDSLPDQVRLVLQHSGA
                     RFVLVEDQEQADKVLSVCDAVPRLDRIIVDDVRGLEGSPDPRLIGLEEVARRGREADA
                     REPGRYDALLERGTPDDVAVLMYTSGTTGVP"
BASE COUNT          672 a         1592 c         1673 g          699 t
ORIGIN      
        1 gtccgcctcg cagccgaagt agtaccgggg cacgaacagc tcgtggatgt cttccttccg
       61 ctcgatcttg cagcggaagt agtcgtcgag gtcctcgatg ccgccggtca ggaccgagtt
      121 cgagtcgcct tccaggccgt ccccgcggcc caccgcctcc actaccgcct cgcggccata
      181 cctctgcgcc agcgcgagca gggccccgcg gtcgagattg cccgggttcg tcgactcgag
      241 cgcctgccgg ttgcgcttct cccagtgccc gatgaggtcc gcgtagagca tgcaggccca
      301 gcccacgccg ccctcgagga aggcgaagtt caggtccggg aaccggcgcg tcacgccgcc
      361 gaagaagagc gccttggcga ccgcgtgacc cgcggaggcg aagtggccga tgtggttgta
      421 gcagaagttc gacggcgagt tgcgcaggag gatcgagcgc gcgccgttgt ggaagctcgg
      481 cgcgacgcgc agctcgcggc acttctccca cacggggtcg tagtcgtggg ggctgtcgat
      541 gccgacgacg tcgtaccact cgacgaagcg cgaggtgtcg ggatgctccg cggcgagggc
      601 cgggaccggg cgacgcatga ggccgccgac catcatgacc ttgtaaccga gctgcttggc
      661 cgcgaagtcc agctcctcga ccgcctcgtc cggcgtgtac atcgggatga tggcggcggg
      721 gatgacacga tcctcgagtc cgcggaactg atcggcggtg aagacgttgt aggcgcggca
      781 gatggcccgc cgcagccttg tgtcctgcat ccggtgatag ctgaggcccg ccgtcggata
      841 gacgacgcag aggtcgatgc cgaggtcgtc gaggcgctcg tacatgaggc ggggcagcat
      901 ggcggtcgcc cggtcgagca cgttccggca gggcgacccc cagaaagcct cctggccgac
      961 ccggcgctgc gtgcgctccg ccagcgacat ccggagcgcg ttcgggaccc gctggctggc
     1021 gacctcgagg gcctcggccg ccgcgtcgcc gccgatgcgc cggaactcct cgcgcatgac
     1081 cggcgcgtac tcgagccagt ggccgtcggc gtcgatgacg gggtggttca ggcgggcgtg
     1141 gagcgtccgg gcgtcctggt gaccattctc gtgcatggcc gcgcctccag gggattcggt
     1201 gaatggtggc gatgatactc ccggagagac gtttcgacga ccgcgccgga gcgggcgcgg
     1261 cggggccggc cgacgctcag agcgtcgggc tcgagaactc gccgccgagc gcgacgagcg
     1321 cccacgggcc cccctgcgcg tcccagctga agccgatgtg ggccgagagc gcgtgctggt
     1381 cgcgaaacag gcgctggatc ggatagcggt cgtagatgcc gttggcgccg gcgagcgcgt
     1441 gcagcgaatc gacggcctcg gtgcacagct gcatcgcata cgccacgttg cgcttgaagc
     1501 ggagcttgtc ctccaccgac ggcacgcggt cctctcgcgc gatgcgctcg gcgtccagac
     1561 agtcctcgcg gatcacgagc cgcgccgcgt cgatcatggc gcccgcccgg ccgacccgga
     1621 gctgcaccgc ctggaagtcg cgcatgcgca tggccgtgta gctggtggag cgctcgcgga
     1681 tcgcctcgat cgtgagctcg agcgcggcct gcgcgttccc gacgccggtg ccggccaggc
     1741 agtgcgagcc gagcccggag agcggcacgc ggtagagcgg attcggattg acgcgcgcgc
     1801 cggggaactc ggcgccgccg cgcgcgaggt acatgcacag cgcccggtgc tcgggcacga
     1861 agatctccgt cgcgcgcacc gacttggagc ccgtgccctg catgcccagc acgtgccagt
     1921 cgtcgacgat ctcgtactcg gtccggggga cgagacacat ccggtggtcg acgacccggt
     1981 cgccctcgcg gaccgtcacc gcgagcatgt tccagtcgca cgcgtccacg ccgctcgaga
     2041 agttccagag gccgctgacg acgaagccgc catcgacctt ccgcccccgg ccctgggggt
     2101 aagcgatgcc cgaggcgatg agggcgtcgg ggttccggcc ccagacctct tcctgggcgc
     2161 gctcgtcgta gagggcgagc atccagtggt ggatgccgag gttgccgatg ttccaggccg
     2221 tcgaggcgca gccgcgcgcg atctccgcgg ggatgtcgag gaccgccacg aagggcagct
     2281 ccatcccgcc ccagcggcgg ggctggtgga accggagcag ccccgcgcgg tggaggtcct
     2341 ccagcgtctc cttcggcatc tggcgcgcgg cctccgtgcc gtccgcgcgc tcgcggagga
     2401 ccgggacgag ggcccgcgcg cggcgcagcg cctcgtcgtg ggtcacgtcg gcgaagccgg
     2461 acctgctcac gcgcgcatcc cggccacctg cggcatcttc gagtagtcct cgccgcccca
     2521 gtccgtcacg tccttgagcc cgaagaagtt cagctcgatc gtcagaccgt tcggatccgt
     2581 gatgaagatc tggtagagat cgaactccgg gagcgaccgt ggccggtagt cgatcccgcg
     2641 ttccttgaag cgccggatga agccgcccgg ctccgtggcc acgaacgcga tgtggtccac
     2701 gacgccggcg tggtccttga ccgcgccctt cggtgttccc aggtagtaga ggtcggcatt
     2761 gtcgatgccg tccggaccca tgtgcacctg gatcttgccg ttcaccccga gccagtagcc
     2821 cggaaagggg aacgtcggcc gcggcatcac ctggaagccg agcacgtcgc agtagaactc
     2881 ccgggtcttc tcgaggtcgt tcgcccggac gaagtagtgg ttcagctctg tcaccggcat
     2941 aggatcctcc ccggctcgtg ccgacgcctg tgggattgta gcgctgctcg ccggtcggga
     3001 ccattcgcgc gccggacacc gcgtgatggg cgtcgccgcc gcccgcggcc gaggtcgacg
     3061 cccgacctat tgacaacggc ccggtactcg gcgtagcgtc cgcgccacct cgtgaagcga
     3121 tcgtcctgcc cctcggggga attcatgccc tggaccgggt tcgtggacac ctcttcgacg
     3181 ccctcgccgg tccccgggac cgcggggaag gctcgcgcgc ggccctcgcc ggcgcgccgc
     3241 ggcgtgtaga ccggatggcg ctgctcgctc tggaggggct gcatctcaac ttcggcggcg
     3301 tggcggctct ggccggcgtg agcctggagg tcgccgccgg cgagttcttc gccgttatcg
     3361 gccccaacgg ggcgggcaag accagcatct tcaactgcat cagcggcatc tatgcgccga
     3421 cgcgcgggcg catccgcttc gacggacggg acatcacgcc gctgccgccc caccagcgcg
     3481 cccggctcag gatcgcgcgc accttccaga acatcgctct cttcaagggc atgagcgtgc
     3541 tggacaacgt gaagatcggg cgccacgttc acctgcgctc ggggcccctg gcctgcgggc
     3601 tctactacgg ccgtgcggcg cgcgaggagc gcgcccaccg cgccaccatc gagcgcgaga
     3661 tcatcgagct gctggagtta caggatattc gccacaagat cgtcggcacg ctgccgtacg
     3721 gcttgcagaa gcgggtggag ctggcccggg cgctggcgct cgacccggtc gtgctgctcc
     3781 tcgacgagcc gactgccggc atgaacgccg aagagaccga ggacatggtg cgcttcgtcc
     3841 tctacgtcaa gcgggtgaag cgtctgaccg tggtgatgat cgagcacgac atgggcgtgg
     3901 tcatggacat ctccgaccgg gtggccgtcc tcgatttcgg gcggaagatc gccgagggca
     3961 cgcccgccga ggtccagcgg gatcccgtgg tgatcaaggc ctacctcggt caggacttcg
     4021 aggtggcgcc cgtggggtag cgccggccgt cgatcgagcg acgttgccgc ggctgctcgc
     4081 cgcccaggcc cggcgcttgg gcggccggaa ggtcgccctg cgcgagaaga agtacggaat
     4141 ctggcaggag gtgacctggg agggctacgc cgcgcacgtg cgcgccgtgt gcatgggtct
     4201 cctcgggctc ggcctcgagc ggggcgaccg ggtagcggtg atctccggga accgcccggc
     4261 ctggctctac accgagctcg cggcgcagac ggccggcgcc atcccgctgg ggatcttcgt
     4321 cgacagcctc ccggatcagg tgcggctcgt cctgcagcac agcggggccc gcttcgtcct
     4381 ggtcgaggat caggagcagg ccgacaaggt cctgagcgtg tgcgacgccg ttccgcgcct
     4441 cgaccggatc atcgtcgacg acgtgcgggg gctggagggg agcccggacc cgcggctgat
     4501 cggcctggag gaggtggcgc ggcgggggcg cgaggccgac gcccgcgagc cgggccgcta
     4561 cgacgcgctg ctcgagcgcg ggacacccga cgacgtggcg gtgctgatgt acacctcggg
     4621 gacgacgggc gtgccc
//
LOCUS       DATIAZ010000088         6471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_415888, whole genome shotgun sequence.
ACCESSION   DATIAZ010000088 DATIAZ010000000
VERSION     DATIAZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415888"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..459
                     /locus_tag="VLF19_03005"
     CDS             118..459
                     /locus_tag="VLF19_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92247.1"
                     /translation="MTTPFVLIAVLAIALMPGAAAGQPPGAQVILDNPSVRVSVITLP
                     PGGGTGRHQGIEAEVGIVVEGDLTVESPLGRQALRPGTAYWLPGLTPHDTRNEGQRPA
                     RMLEILLKRCD"
     gene            668..1873
                     /locus_tag="VLF19_03010"
     CDS             668..1873
                     /locus_tag="VLF19_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSE92248.1"
                     /translation="MKTRWMVLGIAGLLVGGMLGSPRAQSQEPVKIGLVQGITGPFEV
                     YAKQVVEGFKLGLEYGTQGKNEVLGRKIQLLVEDDQLKPDVAKRLVTKLYADDKVDLV
                     VGTTSSAAAIAVLPVAAEFKKLLLVEPAVADSITGEHWNRYVFRTGRNSGQDAIANAL
                     AVARPGVSIATIAQDYAFGRDGVTAYKAAVEKAGAKIVHEEYTPQQATDFTAPIQRIV
                     AAFKDKPAPKYVFVIWAGKGGPFPQLVANRLEKQGITLTSGSNVLDVLKAMKAMGLEG
                     MVGGAYYYHEIPKNAANDWLVREHVKRHSAPPDFFTCGGFSAAMAVLTGLRKAGSTDT
                     EKLVAAMEGMEFETPKGKMTFRKEDHQALQSMYAFRMASKPDVAWLVPTLTRELSPKE
                     TAPPVLNKR"
     gene            1884..2669
                     /locus_tag="VLF19_03015"
     CDS             1884..2669
                     /locus_tag="VLF19_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92249.1"
                     /translation="MTAVGGRPATPAPVLSTRDLTISFGGHVAVDRVSLDVAPCTLKS
                     IIGPNGAGKTTFFNLVSGQYRPTAGRVHFKGRDVTALGAAARTRLGIGRSFQLTNVFP
                     TLSVLENVRLAVQSRRGLGFTVWRDYRAFADLEDRAYAILETVLLEGKWRAAASALSH
                     GEKRKLEVAILLALEPEVLLLDEPTAGMSLEEVPAIIRLVERVKAARDRTILLVEHKI
                     DMILSLSDSVAVLKDGRLIADDTPEAISRNADVQAAYFGGAGG"
     gene            2662..3372
                     /locus_tag="VLF19_03020"
     CDS             2662..3372
                     /locus_tag="VLF19_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92250.1"
                     /translation="MVEGRPILELEDVHTYIGQHHILQGVSLAVRRDAVTVLLGRNGA
                     GKTTTMRTIVGLLRPSAGAVRFDGRPIHGRPPYEIVRLGLGYVPEGQGIFATLTVDEN
                     LRVARLDTRDGEARLRRIFALFPDLERFRRARAGTLSGGQKQMLSIARAFVNDNRLLL
                     IDEPSKGLAPIIVEHLVVALREMKSHATVLLVEQNFAMASALGDDFYLIDDGRTVRRG
                     RMAELVRDEALKKRYLGV"
     gene            3378..4352
                     /locus_tag="VLF19_03025"
     CDS             3378..4352
                     /locus_tag="VLF19_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92251.1"
                     /translation="MRRALLALGALALVLAPLAFMDWRTYLTLTVAGLAMGMLIFLVA
                     AGLTLIFGLMDVLNFAHGAVFSWGAYVGFSAIVLLNGRWGWADTPSVALNGLLLLAAV
                     VAAMGVAVVLGVVLERVIVRPVYGQHMFQILITLGATIVIEELIRIVWGPNDQVMPVP
                     FTFQGSWDVADVIVLRVPVIAIALGLLVYAGIEAVLRGTRVGLIVRAGVESGEMIQAL
                     GHNIYRYFTGVFAAGSALAALGGVMWAVFSQSVRPTMGGEQLIFAFIVVIIGGLGSVT
                     GSLVGALVVGLAYNYVAFLVPKAAIGVNMAIMVLILLLRPTGLFGTAK"
     gene            4349..5440
                     /locus_tag="VLF19_03030"
     CDS             4349..5440
                     /locus_tag="VLF19_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92252.1"
                     /translation="MTLEAPTPVQAQRRAERERRWRDPARWLRIACAVAAALVPLVVP
                     SFQVLDLAAKIALFAALVASYDIVLGYTGIVSFGHAMFFGFGAYGVALALTKFGASTY
                     GHLALGFLAALALSAVVALLIGAFSLRVKAIFFAMITLAFAEFALILAVQLTPITGGE
                     DGLSPRLPGVLAVAWEGESLLGVRLTGRLLTYWLVLAVVLALFVVMARFIRSPLGRTL
                     QAIRDNELRAEALGYPTFVFQSAAAAFASVVASVLGGCYAMWVRYVNPESVLGIHVML
                     DVLLMVIIGGIGTLWGSIIGAAVLLTAQTLLPNLRGLGQALFADSALVQRLTDRWLLY
                     FGILFVLVVFFFPRGVIGTLRDAVARRRA"
     gene            5502..5957
                     /locus_tag="VLF19_03035"
     CDS             5502..5957
                     /locus_tag="VLF19_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HSE92253.1"
                     /translation="MAAGGVACYPSSGMRALTVSGFFARFALALVLVALTYNPTSYSY
                     YSWLRSTGWQWSPVMLLAGIVLLIGWVVYLRATARSLGAIGLVLACAFFAALFWLFTD
                     WGWIPLDSATAITWVAMVMVAAILAIGMSWSHLRRRWAGQADVIDRDER"
     gene            6124..>6471
                     /locus_tag="VLF19_03040"
     CDS             6124..>6471
                     /locus_tag="VLF19_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE92254.1"
                     /translation="MDGVAHGLDDTHSANDAFLEIEDVHLAFGGVRALRGVGFRVQRG
                     QIFSLIGPNGAGKTVLLNCINGLYQPQRGRILFEGRDLVGLKPYRRAALGISRTFQKI
                     ELFGGMTVLDNIRL"
BASE COUNT          880 a         2288 c         2230 g         1073 t
ORIGIN      
        1 agccaggtcc cgatgctcgt gctcatggtc ggctacacga tcgtgagcct ctggatcatc
       61 gcccaaccca tcgtcgagtc gagcccgaaa gggtagcgcc gagaggagga gatcgagatg
      121 acgacgccct tcgtgttgat cgcggtcctc gccatcgccc tgatgcccgg cgcggctgcc
      181 ggccagcccc ccggggcgca ggtcatcctc gacaatcctt cggtgcgcgt cagcgtcatc
      241 acgcttcccc ctggcggcgg gaccgggcgg caccagggga tcgaggcgga agtgggcatc
      301 gtcgtcgagg gagacctgac cgtggagagc cccctggggc gccaggcgct gcggccgggg
      361 acggcctact ggctgcctgg cttgacgccc cacgacaccc gcaacgaagg ccagcggccc
      421 gcgcgaatgc tggagatcct gctgaagcgg tgcgattgac cgtccgaggc cgcgccgcgc
      481 gggatctcac gcggcgtgca tcgcgctcta cggcgtcagg tccatcgcct ccacgatgtc
      541 ccaggtctcg tcgagtcgag cccgagaggt cggtatcccg ccggtcgtgt tgacgcgacg
      601 cgccaacggc gttagattcg cgtcgtcccg cgccggcgcc ggcagtgggc aacaggagga
      661 gcggtcaatg aagactcggt ggatggttct cggaatcgca gggctcctgg tcggcgggat
      721 gctggggtcg ccccgcgccc agagccagga gccggtcaag atcggcctgg tgcaggggat
      781 cacggggccg ttcgaggtgt acgccaagca ggtggtcgag ggcttcaagc tcgggctcga
      841 gtacggcacg cagggcaaga acgaggtgct gggccgcaag atccagctcc tcgtcgagga
      901 cgaccagctc aagcccgacg tggccaagcg gctggtcacc aagctctacg ccgacgacaa
      961 ggtggacctc gtcgtgggca cgacgtcgag cgccgcggcc atcgccgtgc tccccgtcgc
     1021 cgccgagttc aagaagctgc tgctcgtcga gcccgccgtg gccgactcca tcaccggcga
     1081 gcactggaac cgctacgtct tccgcacggg ccgcaactcc ggccaggacg ccatcgccaa
     1141 cgcgctggcc gtggcccggc ccggcgtgtc gatcgccacg atcgcccagg actacgcctt
     1201 cggccgtgac ggcgtgacgg cgtacaaggc ggccgtcgag aaggccggcg cgaagatcgt
     1261 gcacgaggag tacacgcccc agcaggccac cgacttcacc gcccccatcc agcgcatcgt
     1321 ggccgccttc aaggacaagc cggcgccgaa gtacgtcttc gtcatctggg ccggcaaggg
     1381 cgggccgttc ccgcagctcg tcgccaatcg cctcgagaag caggggatca cgctgacgag
     1441 cggcagcaac gtgctcgacg tcctcaaggc catgaaggcc atggggctcg agggcatggt
     1501 gggcggcgcc tactactatc acgagatccc gaagaacgcg gcgaacgact ggctcgtccg
     1561 cgagcatgtc aagcgccaca gcgcgccgcc ggacttcttc acctgtggcg gcttctcggc
     1621 ggccatggcc gtcctgaccg ggctccgcaa ggccggctcg acggacaccg agaagctcgt
     1681 tgccgcgatg gagggcatgg agttcgagac gcccaagggc aagatgacct tccgcaagga
     1741 ggaccaccag gccctgcagt cgatgtacgc gttcaggatg gcgtccaagc cggacgtggc
     1801 ctggctcgtg ccgacgctca cgcgcgagct gagcccgaag gagacggcgc cgcccgtgct
     1861 gaacaagcgg taaccgggcg gcgatgacgg cggttggggg ccggccggcg acgccggccc
     1921 ccgtcctctc gacccgcgac ctcaccatca gcttcggcgg ccacgtcgcc gtcgatcggg
     1981 tctcgctcga cgtcgccccc tgcacgctga agtcgatcat cggtcccaat ggcgccggca
     2041 agacgacctt cttcaacctc gtgtccggcc agtaccgccc gaccgcgggc cgcgtccact
     2101 tcaagggccg cgacgtgacc gcgctgggcg ccgccgcccg gacccgcttg ggcatcgggc
     2161 ggtcgttcca gctgaccaac gtcttcccca cgctgagtgt gctggagaac gtccggcttg
     2221 ccgtgcagtc ccgccgcggc ctcggcttca ccgtgtggcg cgattaccgc gcgttcgccg
     2281 acctggagga ccgcgcctac gcgatcctcg agacggtgct gctcgagggc aagtggcggg
     2341 cggctgccag cgcgctgagc cacggcgaga agcgcaagct ggaggtggcc atcctgctcg
     2401 cgctggagcc cgaggtgctg ctgctggacg agcccacggc gggcatgtcg ctggaggagg
     2461 tgccggcgat catccggctc gtcgagcgcg tcaaggccgc gcgggatcgg acgatcctgc
     2521 tcgtcgagca caagatcgac atgatcctgt cgctctccga ctccgtcgcc gtgctcaagg
     2581 acggccggct catcgccgac gacacgccgg aggccatcag ccgcaacgcc gacgtccagg
     2641 ccgcgtactt cgggggcgcc ggtggttgag ggccgaccga tcctcgagct ggaggacgtg
     2701 cacacttaca ttggccagca ccatatcctg cagggcgtgt cgctcgccgt gcggcgcgac
     2761 gccgtcaccg tgctcctggg gcgcaacggc gccggcaaga cgacgaccat gcggacgatc
     2821 gtggggctgc tgcggcccag cgccggcgcc gtgcgcttcg acgggcggcc gatccacggc
     2881 cggcccccgt acgagatcgt ccgcctgggg ctcgggtacg tgcccgaagg gcaggggatc
     2941 ttcgcgaccc tgaccgtgga cgagaacctg cgcgtggccc ggctcgacac ccgcgacggc
     3001 gaggcgcgcc tgcggcggat cttcgcgctc ttccccgacc tcgagcgctt ccggcgcgcc
     3061 cgcgcgggca cgctctccgg tggccagaag cagatgctct cgatcgcgcg cgccttcgtg
     3121 aacgacaatc gcctgctcct gatcgacgag ccgtccaagg gactggcgcc catcatcgtc
     3181 gagcacctcg tcgtcgccct gcgggagatg aagagccacg ccaccgtgct gctcgtcgag
     3241 cagaacttcg ccatggcgtc cgcgctgggc gacgacttct acctgatcga cgacgggcgg
     3301 acggtgcgcc ggggccgcat ggccgagctc gtccgcgacg aggccctcaa gaagcggtac
     3361 ctgggagtgt gatggcgatg aggcgtgcgc tcctcgccct cggcgccctg gccctcgtcc
     3421 tggcgccgct cgccttcatg gactggcgca cgtacctgac cttgaccgtc gccgggctgg
     3481 ccatgggaat gctgatcttc ctcgtcgccg ccggcctcac gctgatcttc ggcctcatgg
     3541 acgtcctgaa cttcgcccac ggcgccgtct tttcctgggg cgcctacgtg gggttcagcg
     3601 ccatcgtcct gctgaatggc cgctggggat gggccgacac gccgtccgtc gcgctcaacg
     3661 gcttgctcct gctcgccgcc gtcgtcgccg ccatgggcgt cgcggtggtg ctgggggtgg
     3721 tgctcgagcg ggtcatcgtc cggccggtgt acgggcagca catgttccag atcctcatca
     3781 cgctgggcgc gaccatcgtg atcgaggagc tgatccgcat cgtgtggggg ccgaacgacc
     3841 aggtgatgcc ggtgcccttc acgttccagg gctcgtggga cgtcgccgac gtcatcgtgc
     3901 tgcgcgtgcc cgtgatcgcc atcgcgctcg gcctcctggt ctacgccggc atcgaggcgg
     3961 tgctccgcgg cacccgcgtg gggctcatcg tgcgcgccgg ggtcgagagc ggcgagatga
     4021 tccaggccct cggccacaac atctaccgct acttcaccgg cgtcttcgcc gcgggctcgg
     4081 ccctggccgc gctgggcggg gtgatgtggg ccgtgttcag ccagtccgtc cggcccacca
     4141 tgggcggcga gcagctcatc ttcgccttca tcgtcgtgat catcggcggg ctgggcagcg
     4201 tgacgggctc gctcgtcggc gccctcgtcg tcgggctcgc ctacaactac gtggccttcc
     4261 tcgttcccaa ggcggccatc ggggtgaaca tggccatcat ggtcctgatc ctcctgctgc
     4321 gtcccaccgg actgttcgga accgccaagt gacgctcgaa gcgcccacgc cggtccaggc
     4381 ccagcgccgg gccgagcgcg agcggcgctg gcgcgacccc gcgcggtggc tccggatcgc
     4441 ctgcgccgtg gcggcggcgc tcgtgccgct ggtcgtcccc tcgttccagg tgctcgacct
     4501 cgcggccaag atcgccctgt tcgccgcgct ggtggcgagc tacgacatcg tgctgggcta
     4561 tacgggcatc gtctcgttcg ggcacgcgat gttcttcggc ttcggcgcct acggggtggc
     4621 gctcgccctg acgaagttcg gcgcctcgac ctacggccac ctggccctcg gcttcctggc
     4681 cgccctggcg ctgtccgccg tcgtggcgct gctgatcggc gccttctcgc tgcgcgtgaa
     4741 ggccatcttc ttcgccatga tcacgctggc gttcgccgag ttcgcgctga tcctcgccgt
     4801 ccagctcacg ccgatcacgg gaggggagga cggtctgagc ccccggctgc ccggggtgct
     4861 ggcggtggcg tgggaggggg aaagtctgct cggcgtccgg ctgaccggcc gcctgctcac
     4921 gtactggctg gtgctcgccg tggtcctcgc gctcttcgtc gtgatggcga ggttcatccg
     4981 ctctccactg gggcggacgc tgcaggccat ccgcgacaac gagctgcgcg ccgaggcgct
     5041 gggttacccg acgttcgtgt tccagtccgc cgccgccgcc ttcgccagcg tggtcgcgag
     5101 cgtgctcggc ggctgttacg cgatgtgggt gcgctacgtg aaccccgagt ccgtcctcgg
     5161 catccacgtg atgctcgacg tgctcctcat ggtcatcatc ggcggcatcg gcaccttgtg
     5221 gggctcgatc atcggggccg ccgtgctgct caccgcccag accctgctgc ccaacctccg
     5281 cggtctgggc caggcgctct tcgccgactc cgcgctcgtg cagcggctca ccgaccgctg
     5341 gctgctctac ttcggcatcc tcttcgtcct cgtcgtcttc ttcttccccc ggggcgtgat
     5401 cggcacgctg cgggacgccg tagcgcggcg gcgggcgtga ggtcgccgga ggcacggccg
     5461 gtccacccgc ggccggccgg ccgagggagc gcgccgtgcc gatggcagcc gggggcgtgg
     5521 cctgctaccc ttcgtccggc atgcgcgcgc tgacggtctc ggggttcttc gcgcggtttg
     5581 ccctcgccct ggtgctggtc gcgctcacgt acaacccgac gtcctactcc tattattcgt
     5641 ggctgcgcag cacgggctgg cagtggagtc ccgtgatgct gctggccggg atcgtgctgc
     5701 tgatcggctg ggtggtgtac ctgcgcgcga cggcccgctc gctcggcgcg atcggtctcg
     5761 tgctggcgtg cgcgttcttc gccgcgctgt tctggctctt caccgactgg ggctggattc
     5821 ctctcgacag cgccacggcc atcacgtggg tggccatggt catggtcgcc gcgatcctgg
     5881 ccatcggcat gtcgtggtcg cacctgcgcc ggcgctgggc ggggcaggcg gacgtcatag
     5941 acagggacga gaggtgacgg gggcacgtgt cgcgcggccg gtcaacgggc cgcgtgattc
     6001 gctgtaagtc cccgtcattt cgtcccaatt gcttgacagc ccgcggacgg tccgctagac
     6061 tctcgccact cgaacggagc ccgccggaca accggaattt gccaaacccg tcgcggaggc
     6121 gacttggacg gagtcgcgca cggcctggat gacacgcact cggcgaacga cgcctttctc
     6181 gagatcgagg acgtccatct cgccttcggc ggtgtccgcg cgctgcgcgg tgtcggcttt
     6241 cgcgtccagc gggggcagat cttctcgctc atcggcccca acggcgccgg caagaccgtc
     6301 ctcctgaact gcatcaacgg gctctaccag ccgcaacgcg ggcgcatcct cttcgaaggc
     6361 cgggacctgg tcggcctcaa gccctaccgg cgcgcggcgc tcggcatctc gcgcaccttc
     6421 cagaagatcg agctgttcgg cgggatgacg gtgctcgaca acatccgcct c
//
LOCUS       DATIAZ010000089         3182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1600163, whole genome shotgun sequence.
ACCESSION   DATIAZ010000089 DATIAZ010000000
VERSION     DATIAZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600163"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VLF19_03045"
     CDS             <1..224
                     /locus_tag="VLF19_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92255.1"
                     /translation="RITTGNVGLAWAHDIESCTARGLEATPIVLDGAMVRNGMVAFSR
                     YLEPDDVETIRAYVIQRAHDEQKRRADTR"
     gene            complement(268..1026)
                     /locus_tag="VLF19_03050"
     CDS             complement(268..1026)
                     /locus_tag="VLF19_03050"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92256.1"
                     /translation="MVYRAASGPVEALRDITFSVGQGELCALVGPSGCGKSTLLRLVA
                     GLRAATAGEVRVAGRPVTRPIPEVGMVFQAPVLLKWRRVLDNVLLPAELAGREPRQYE
                     RRARDLLALVGLGEFADKLPRELSGGMQQRAALCRALLLDPPLLLMDEPFGALDAMTR
                     DEMNVELLRVWGEGSRERKTVVFVTHSIPEAVFLADRVVVMTPRPGRVARIFPVDLPR
                     PRTVETRGSPAFGRLSLEIYETLTATGVRAPMSG"
     gene            complement(1047..2066)
                     /locus_tag="VLF19_03055"
     CDS             complement(1047..2066)
                     /locus_tag="VLF19_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92257.1"
                     /translation="MARTPIVVVAALLVAVTLGAAQAQAPQKVVFALNWFAVGDHAAY
                     WVAVDKGYYKARGLDVELQNSKGSGDSIAKVDTGRADIGLADAVVVIPRVAQGAKIRI
                     VGAVFDNTPLNIWTRKDTGIGKPKDLEGKTLAAPPGDSQRQLFPAFAKINGVDESKVT
                     WVTIEPAAKMVALAKKRVDAVPDYTTGQPLFEKAFGEEGLPRDTLVRMPWNQYGFDTY
                     SMSIFASEKTINERQKVLRDFLAASYMGWRDVMDNPKAAMEIFKRRVPEIDAAVIEPN
                     LMLGLELMRTDRYAKNGIGWMDRGKMCRTVEVINSYMPNMPRKVGCDEVFTNDFLTKI
                     EPPRR"
     gene            complement(2084..2842)
                     /locus_tag="VLF19_03060"
     CDS             complement(2084..2842)
                     /locus_tag="VLF19_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92258.1"
                     /translation="MTAQTLRRYLPSFALFGALLLAWQLAGPVFQIREYLLPSPASVV
                     RAALGWSVPWHTHLTITTLEILGGFAVAGAGGVVLGIAIAWSPTIARALVPFLVFVNT
                     LPKVAVAPLFLLWLGYGIVPNILIAALIGFFPVVINTAAGLTQVDEELLDLGRVFGAP
                     TWKVFLKIRLPNALPYVLSALKITATAAVVGAVVGEFVASQAGLGMVIVNAQTNLNTP
                     VAFAALVWISVVGLLLYGAVGLAARRWAPWADTT"
BASE COUNT          501 a         1102 c         1129 g          450 t
ORIGIN      
        1 ccaggatcac caccggcaac gtcgggctcg cctgggccca cgacatcgag agctgcaccg
       61 cgcgtggtct cgaggcgaca ccgatcgtcc tcgacggcgc gatggtccgc aacgggatgg
      121 tggcgttcag ccgctacctc gagcccgacg atgtggagac gatccgtgcg tacgtgatcc
      181 agcgcgccca cgacgagcag aagcggcgcg ccgacacccg ctgaggcggt cgagagggcg
      241 ccgctcccgc ggggatgtcg cggaagctca gcccgacatc ggagcccgca cccccgtggc
      301 cgtgagcgtc tcgtagatct ccagcgacag tcgtccaaac gccggcgagc cacgcgtctc
      361 caccgtgcgc ggccgcggca ggtccacggg gaagatcctc gccacgcggc ccggtcgcgg
      421 cgtcatcacc acgacgcggt cggcgaggaa gacggcctcg gggatcgagt gggtcacgaa
      481 cacgacggtc ttgcgctcgc ggctgccctc gccccagacc cggagcagct ccacgttcat
      541 ctcgtcgcgc gtcatcgcgt ccagcgcgcc gaagggctcg tccatcagca gcaacggcgg
      601 gtccagcagg agggcccggc agagcgcggc gcgctgctgc atgccgcccg agagctcgcg
      661 cgggagcttg tcggcgaact cgccgaggcc gacgagcgcc agcaggtcgc gggcgcgccg
      721 ctcgtactgg cggggctcgc ggccggccag ctcggcgggc agcagcacgt tgtcgagcac
      781 gcggcgccac ttgagcagca cgggcgcctg gaagaccatg ccgacctcgg ggatcggccg
      841 cgtgaccggt cgaccggcga cccgcacctc gccggcggtg gccgcgcgca gcccggccac
      901 gagacggagc agcgtggact tgccacaacc ggacgggccg accagggcgc agagctcgcc
      961 ctggccgacc gagaaggtga tgtcgcgcag ggcctcgacc gggcccgagg cggcccggta
     1021 gaccatgccg acatgctcga ggacgatcaa cgcctgggcg gctcgatctt cgtcaggaag
     1081 tcgttggtga agacctcgtc gcagcccacc ttgcgcggca tgttcggcat gtagctgttg
     1141 atcacctcga ccgtccggca catcttgccg cggtccatcc agccgatgcc gttcttcgcg
     1201 tagcgatcgg tccgcatcag ctcgagcccg agcatcaggt tgggctcgat gaccgccgcg
     1261 tcgatctcgg gcacgcgcct cttgaagatc tccatggcgg cctttggatt gtccatgacg
     1321 tcgcgccagc ccatgtacga ggcggccagg aagtcgcgca gcaccttctg acgctcgttg
     1381 atggtcttct cgctggcgaa gatcgacatc gagtacgtgt cgaagccgta ctggttccac
     1441 ggcatgcgga ccagcgtgtc cctgggcagg ccctcctcgc cgaacgcctt ctcgaagagc
     1501 ggctgtccgg tcgtgtagtc cggcaccgcg tccacccgct tcttggccag cgccaccatc
     1561 ttggcggccg gttcgatggt cacccacgtg accttcgact cgtccacgcc gttgatcttg
     1621 gcgaaggcgg ggaagagctg gcgctgggag tcgccggggg gcgcggccag ggtcttgccc
     1681 tccaggtcct tgggcttgcc gatgcccgtg tccttgcgcg tccagatgtt gagcggcgtg
     1741 ttgtcgaaca cggcgcccac gatcctgatc ttcgccccct gcgccacgcg cgggatgacg
     1801 acgacggcgt cggcgagccc gatgtcggcg cggcccgtgt ccaccttggc gatggagtcg
     1861 ccggagccct tcgagttctg cagctccacg tccaggcccc gggccttgta gtagcccttg
     1921 tcgacggcca cccagtacgc ggcgtggtcg ccgaccgcga accagttcag cgcgaagacg
     1981 accttctgcg gcgcctgcgc ctgggccgcg cccagggtca cggccaccag cagcgcggcg
     2041 acgaccacga tcggcgtgcg ggccatgatg ctctccttcc agctcacgtg gtgtcggccc
     2101 agggcgccca ccggcgcgcg gcgaggccga ccgcgccgta gagcaggagg ccgaccacgg
     2161 agatccacac cagcgcggcg aaggcgacgg gcgtgttgag gttggtctgg gcgttgacga
     2221 tgaccatgcc gaggccggcc tgcgatgcca cgaactcgcc cacgacggcg cccaccacgg
     2281 cggccgtcgc ggtgatcttc agcgccgaca gcacgtaggg cagggcgttg ggcaggcgaa
     2341 tcttgaggaa caccttccac gtcggcgcgc cgaagacgcg cccgaggtcg aggagctcct
     2401 cgtcgacctg ggtgaggccg gcggcggtgt tgatgacgac ggggaagaag ccgatcagcg
     2461 cggcgatgag gatgttgggg acgatgccgt agccgagcca gaggaggaac agcggggcca
     2521 cggccacctt gggcagcgtg ttcacgaaga cgaggaacgg caccagggcc cgcgcgatgg
     2581 tcggcgacca ggcgatggcg atgccgagca cgacgccgcc ggcgccggct acggcgaaac
     2641 cgccgaggat ctcgagcgtg gtgatggtga gatgcgtgtg ccacggcacc gaccagccga
     2701 gcgccgcacg caccacgctg gccgggctcg gcagcaggta ctcgcggatc tggaacaccg
     2761 ggccggccag ctgccaggcg aggaggaggg cgccgaagag cgcgaatgag ggaaggtaac
     2821 gtctgagggt ctgcgccgtc acggcgcggt catgatagca gcgggccctg ggccccgcca
     2881 cggccgtcgg ccccaggcct cgaccagtga cacctacgcg cgcgatcacg ccgagctcct
     2941 cttgcgcctg caggcgcccc gagtgccggc cgaaaatagc acggcccgtg gcgtcgcccg
     3001 cgagcggccc gggagatctg cgtcgccggc tacctgatca gcccgtagaa cttgccggcg
     3061 ttctcgcagg tgatcttgtg gaccacgtcg gccggcaggt ggccgaactg ctcctcgatg
     3121 tacttcgagg actccggcca cacgccgtcg gtgtgcgggt agtcggacgc ccacatgagc
     3181 gt
//
LOCUS       DATIAZ010000090        10459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1864065, whole genome shotgun sequence.
ACCESSION   DATIAZ010000090 DATIAZ010000000
VERSION     DATIAZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864065"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1072)
                     /locus_tag="VLF19_03065"
     CDS             complement(86..1072)
                     /locus_tag="VLF19_03065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSE92259.1"
                     /translation="MSDAVIVRLPNWLGDTVMAVPALAALRRSLDGARLGVAGPWASL
                     LAGQGLADVLVTYPRGWGGRLRAADAVRAIDATTAVLLPNSFEAAAAALYWGARRRVG
                     FATGGRRPLLTDAPPLPAPRLHQVDEYLLLVERLGIAPGERTPRLRPPSPDSPERVAA
                     RALLAEAGAAGGPLVGLHLGAAFGPSKLWPVERMAELARVLSARGRTPILLGAAGDAV
                     LAAAVTARASAASLVGRDSPALLPAVLAEIDALVCGDTGVGHLAAALGTPVVTLFGPT
                     DARLTAPRGPAVVVSKPTPCAPCFYRTCPIDHPCLGAITAGDVAERLAEVGI"
     gene            complement(1057..2154)
                     /gene="lpxK"
                     /locus_tag="VLF19_03070"
     CDS             complement(1057..2154)
                     /gene="lpxK"
                     /locus_tag="VLF19_03070"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HSE92260.1"
                     /translation="MRREDASALLTRGWDRGFAPAPALVLGALAGGYRRFLGTRDWLY
                     ATGVLRSRRLDCAVVSVGNLTVGGTGKTPAVELAVHTLTALGRRAAVVSRGYRRRSRG
                     VQVVADTAAIRLDAEDAGDEPFLLARRLPGVPVVVGASRYQAARLAVERFGVDAVVID
                     DGLQHRTLDKDLEIVTVRAQRPWGNGRLLPGGPLREPLRALARADLVVATGAEHPGDA
                     SEVVDTVRHHAPGVPVLPARYVAVECLEAGRMRVEPVGALGGTRLLAFAGIASPAAFE
                     ATCTGLGVRLVDSVSFADHHWYTRDDIRRLGERARALGAAGLVTTEKDWVRLRGLPLP
                     PVPLYVITIRLELLEGAAAWRRVFERACPTR"
     gene            complement(2151..3428)
                     /locus_tag="VLF19_03075"
     CDS             complement(2151..3428)
                     /locus_tag="VLF19_03075"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HSE92261.1"
                     /translation="MIHGVYTLVLAALSVGYLPVALWRRAAHGVPLHLKARLGFGARP
                     VTGPAAWVHAVSVGEAIAAQALLDGLRRAYPALALVVTTVTDTGARVVRDRYAGVAAH
                     RFFPVDLPGAVRRAFRAIHPAFLICMETEIWPNVLRHAARQRVPVMIANGRLSDRSFA
                     RYRLARPLLRVVLDDVSVFAMQSQEDARRMIALGARPERVFVTGNLKHDALPDAAGIV
                     DLWRRLLALRPDQRVWIAGSTHRGEDEAVLAAHRAARAECPGLVLILAPRHPERVGEI
                     VSLINARGWATVRRSELPRGSIGDAVIVLDTVGELAQLYAVADVVFVGGSLVPAGGHN
                     MLEPALRKKPVLFGPHTGNFREVAALLLESGGGIAVRDEAELAAEVLRLLADAALRDK
                     LGAAGLEAAAARHGAVRATLELVERFLAPRTTP"
     gene            complement(3425..4075)
                     /locus_tag="VLF19_03080"
     CDS             complement(3425..4075)
                     /locus_tag="VLF19_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSE92262.1"
                     /translation="MKAQLIAMLAASVVRLLGRTLRLQVVGAERVRPLWEAGRPVIYA
                     AWHGRVLMMPWASARLRRTHGARRVSVLASRSRDGELVARYAARFGVAAVRGSSSRGG
                     AGGLRALVDAVRNGEDVALVPDGPRGPRGRLKEGVVVLAALTGAPVVPLAIATRPARR
                     LASWDAFLVPLPFARCALVLGQPICVPADAGRERAAKDIAQALDETTAAADRLVAS"
     gene            complement(4072..5304)
                     /gene="lpxB"
                     /locus_tag="VLF19_03085"
     CDS             complement(4072..5304)
                     /gene="lpxB"
                     /locus_tag="VLF19_03085"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSE92263.1"
                     /translation="MRPAEGLRPTTIMLSAGEASGDGHGALLCRALRSQAPELTLLGL
                     GGARMAAEGVDLVADVTRRAAVGTTEALGRIPALWRTFRVLRDRLERERPAVLVLIDF
                     PEFNLRLARAAHRAGVPVVYFVPPQIWAWRAWRVRAIRRWVSLVLAALPFEPAIYRRA
                     GVPVEFVGHPVLDALGHAPSRAEARERLGVPSGALLIGLLPGSRAEEVGRMAPLMREA
                     ARRIAAAHPEATFALGLASTVGAGPVRGALAGGPPVRIVADDSHAVIRAADLLLATSG
                     TVTLEAALLGTPLIVCYRVSAATELLGRVLLRVPWIGLTNLTLGREAVPELYERRHAT
                     PERLASAALRLLDTPGELDAQRAAFVELRSLCGEPGVGARAARHVLSIGGVFDGSLRG
                     LPQHASRGQSPRSNVARS"
     gene            complement(5291..6103)
                     /gene="lpxI"
                     /locus_tag="VLF19_03090"
     CDS             complement(5291..6103)
                     /gene="lpxI"
                     /locus_tag="VLF19_03090"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HSE92264.1"
                     /translation="MERRLGLMCGAGPLPAQMAQAARRRGWRVVAFAFPGAEEMACHA
                     DRVVPSRITEAAAVLATLQAEGATAALFGGRFSMSEVLRADAASADQASQRIGEHAGS
                     RMDAKLLEAIVAMFASIGVEVLDQRPFLGDLAAPGCLTARRPSDAEVADVRRGLAVAH
                     LSADAGIGQTVVVRHGVVTAVEAVEGTTAAIRRGTLLAGPGAVVVKAVARDHDYRFDT
                     PAVGAETVAAAAEGRAAVIAVESGRVLVVDPGAAARAADALGIALVSVDATG"
     gene            complement(6082..7110)
                     /locus_tag="VLF19_03095"
     CDS             complement(6082..7110)
                     /locus_tag="VLF19_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSE92265.1"
                     /translation="MTRGDHRTRAGVVGVGHMGQYHALVYAELWDVDLVGVVDIDAGR
                     AAAVARHYDTRAFADHRDLVGKVDVVSVAVPTEQHFHVARDLLEAGIGVLVEKPITPT
                     LEEARELFAIARRSGAALQVGHVERFNGAVQELRKIVEQPILVESRRLGPFVSRVQKD
                     TVVMDLMIHDLDIVLALVPSPPQRLAAFGAAVHSDVTDVANVQLGFQSGTIATLTASR
                     VTEEKIRTLAITQPDAYIVLDYLVQDIQIHRRAAQESLPNRESIRYRQASFVEHLFVH
                     KDNPLKLEVLHLVRTARRLAAGEAVELAEVEDVRSLAMALEIERLIRDRRPEIEFVPD
                     WPWSAGSA"
     gene            complement(7107..7958)
                     /gene="lpxA"
                     /locus_tag="VLF19_03100"
     CDS             complement(7107..7958)
                     /gene="lpxA"
                     /locus_tag="VLF19_03100"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSE92266.1"
                     /translation="MTERIHATAVVDPRAEIGENATVGAFCVVGPGVTLGRGVEVGHH
                     VVLEGRVVIGPDVRIGHGAALGGAPQDLKFDPATPSGVRVGSGTVIREYVTIHRATRA
                     ESWTEIGPRCLLMGLTHVGHDCRLGEGVILVNYAGLSGHCELEDFVTIGGQSGCPPFT
                     RVGTYGYLGGFSKLEADLPPYMLANGVPATVHGINNVGLRRAGMSAAERRALKDAHRI
                     LYRAGLAPGPALARLRKEAPAGAAVERLIAFVAASTRRGICAPPRGWRDRGPSLAEEA
                     ESEQVSR"
     gene            complement(7955..9013)
                     /gene="lpxD"
                     /locus_tag="VLF19_03105"
     CDS             complement(7955..9013)
                     /gene="lpxD"
                     /locus_tag="VLF19_03105"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSE92267.1"
                     /translation="MEHTPGFTLGELAAALGLTLEGDAMRSVTGVAALEAAGPDDITF
                     VTDARHRDSALASRAGAFLAPPDLVGLPGPVLRATAPRLVLADLLAMFHPPAAAPPGV
                     APSAIIGAGARVAATASVGAFAVVEAGAVIGERVRVHALAYVGAGAEIGEDSVLHPHV
                     VVRERVRIGRRVIVHAGAVLGADGFGYAFDGARHRKIPQVGGVRIEDDVEIGANTTID
                     RATLGETVVRRGTKIDNLVMVAHNVDIGEDCIVAAQSGIAGSSRIGRGVIMLGQAGVA
                     DHVTIGEGAILAAKTGVSQDVPAGEKVFGTWARPIVQARRIWIAEADLPEMVRRVRTL
                     ERRLADLEARLEKEGGRP"
     gene            complement(9018..9578)
                     /locus_tag="VLF19_03110"
     CDS             complement(9018..9578)
                     /locus_tag="VLF19_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSE92268.1"
                     /translation="MRHTLTAALVFVSAVAAGMLTFDGAARAQAPSVTRIGFIDIQRV
                     LVRSAAGVAAREQLEKEKATMQKEMDARRQEFEKLREELEKKGALMSPDARREREETI
                     ERKRRDAARLADDLQRELKRKEQQLIVKVRQELQGIIDKLGKQKGYYLILDGRNAGVI
                     YWAPEADLTDEIIRAYDQEAATRGKK"
     gene            complement(9589..>10459)
                     /locus_tag="VLF19_03115"
     CDS             complement(9589..>10459)
                     /locus_tag="VLF19_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSE92269.1"
                     /translation="SMRIRIGGQTQQGIISFTEPWLFDRPLAAGFDLFNLRRVYDDYD
                     YDSLGGGLRLSHPFAEYWRWTTGYRLTRDDIGDVSASASSALREEEGTRITSAVSGGV
                     TRDSRDSILLPTRGGLTTLTVDVAGFGGDSQFVKTIFNTSYFKPIWFGHVLSARAEVG
                     YGLGWGDEDLPLFERFFLGGPNSVRSFKFRELSPVDETGTRIGGTTELLGNVEYLVPL
                     PFNIRLAAFFDIGQVYGFTTSFDITDLRKAAGMGVRWNSPFGPIRIDYGFNLDRRAGE
                     DVGAVHFSVGTPF"
BASE COUNT         1504 a         3906 c         3767 g         1282 t
ORIGIN      
        1 aagtcccgtc acgaggcgga tgacgggatc cgcgctgccc gtccaccagc ggttcgaggc
       61 caggtgcagg accgtcaggc ccacgtcaga tccccacctc cgcgagacgc tcggccacgt
      121 ccccggccgt gatcgcgccc aggcacgggt ggtcgatcgg acaggtgcga taaaagcagg
      181 gcgcgcaggg cgtgggcttg ctcacgacga ccgccggccc gcggggtgcg gtcagcctcg
      241 cgtcggtggg gccgaacagc gtgaccacgg gcgtgccgag cgcggcggcg aggtggccta
      301 ccccggtgtc gccgcagacg agcgcgtcga tctccgcgag caccgctggc aggagcgcgg
      361 ggctgtcgcg gccgaccagg ctcgccgccg acgcgcgtgc ggtgacggcg gcggcgagga
      421 cggcgtcgcc ggcggcgccc agcagaatcg gagtccggcc tctcgccgag agcacgcgcg
      481 cgagctcggc cattcgttcg acgggccaga gcttcgacgg gccgaacgcc gcgccgaggt
      541 gcagccccac cagcggtccg cccgctgccc cggcctcggc cagcaaggcc cgggcggcaa
      601 cgcgctcggg cgagtccggt gagggcggtc gaagccgggg ggtgcgctcg cccggagcga
      661 tgccgagccg ctcgacgagc agcaggtact cgtccacctg gtgaaggcgc ggcgctggca
      721 gcggcggcgc gtcggtcagg agcgggcgcc gtccgcccgt cgcgaagccc acgcgtcggc
      781 gcgcgcccca gtagagcgcg gccgccgccg cctcgaacga gttcggcagg agcaccgccg
      841 tggtggcatc gatggcgcgg accgcgtccg ccgcgcggag ccggccgccc caccctctcg
      901 gataggtcac gaggacgtcg gccagtccct gtcccgccag gagcgatgcc cacggcccag
      961 ccacccccag gcgcgcgccg tcgaggcttc gccgcagcgc ggcgagcgcc ggcacggcca
     1021 tcaccgtgtc accgagccag ttgggcaggc ggacgatcac cgcgtcggac atgctcgctc
     1081 gaacacacgg cgccaggccg cggcaccctc gagcagctcg aggcggatcg tgatgacgta
     1141 gagcggcacc ggcggcagcg gcaggccgcg tagacgcacc cagtccttct cggtcgtcac
     1201 gagcccggca gctccgaggg cgcgggcgcg ctcgccgagc cggcggatgt cgtcgcgcgt
     1261 gtaccagtgg tggtcggcga acgagacgga atcgacgagc cgcacgccga ggcccgtgca
     1321 cgtcgcctcg aacgccgccg gcgacgcgat gccggcaaag gcgagcagcc gcgttccgcc
     1381 gagcgcgccc acgggctcga cccgcatgcg gccggcctcg agacactcga ccgccacgta
     1441 gcgcgccggc agcaccggga cgccgggcgc gtggtgtctc actgtgtcca cgacctccga
     1501 cgcgtcaccc gggtgctcgg cacccgtggc gacgacgaga tccgccctgg cgagggcccg
     1561 gagcggctcg cggagggggc cgccggggag caggcggccg ttgccccatg gtcgctgcgc
     1621 ccgcaccgtc acgatctcga ggtccttgtc gagcgtgcgg tgctggaggc cgtcatcgat
     1681 gacgacggcg tcgaccccga atcgctcgac ggccaggcgt gccgcctggt agcggctggc
     1741 ccccaccacc accggcacac ccggcagccg tcgcgccagc aggaacggtt catcgccggc
     1801 gtcctcggca tccaggcgga tggcggcggt gtcggccacc acctgaacgc cgcggctccg
     1861 ccgccgatag ccgcggctca ccacggccgc ccggcggccc agcgcggtga gcgtgtgcac
     1921 ggcgagctcg accgccggcg tcttgccggt gccgccgacg gtgaggttgc cgacggagac
     1981 cacggcgcag tcgagcctcc gcgagcgcag gacgcccgtc gcgtagagcc agtctcgcgt
     2041 cccgaggaat cggcggtagc cgcccgccag tgcgccgagg acgagcgccg gtgcaggcgc
     2101 gaagccacgg tcccagccgc gggtcagcag ggcgctcgcg tcctcgcgcc tcacggcgtc
     2161 gtccgcggcg ccaggaagcg ctccaccagc tccagggtcg cgcggaccgc cccgtgacgg
     2221 gcagccgcgg cctcgaggcc tgccgccccg agcttgtcgc gcagcgccgc gtccgcgagg
     2281 agccgcaaga cctcggcggc gagctcggcc tcgtcgcgca ccgcgatgcc cccgccgctc
     2341 tccaagagca gcgcggcgac ctcgcggaag tttccggtgt gcggaccgaa gagcaccggc
     2401 ttcttgcgca gcgccggctc gagcatgttg tgacccccgg ccggcacgag gctgccgccc
     2461 acgaacacga catcggccac ggcgtagagc tgcgcgagct cgccgacggt gtcgagcacg
     2521 atcaccgcat ccccgatgga gccgcgcggc agctcgctgc ggcggaccgt cgcccagccc
     2581 cgcgcgttga tcaggctcac gatctctccg acgcgctccg ggtgacgggg cgccaggatc
     2641 agcacgagcc cgggacactc agcccgcgca gcgcgatgag ccgcgagcac ggcctcgtcc
     2701 tcgccccggt gggtgctgcc ggcgatccac acccgctggt cggggcgcag ggcgagcagc
     2761 cgtcgccaga ggtcgacgat gccggcggcg tccggcagcg cgtcgtgctt gaggttgccc
     2821 gtcacgaaga cacgctcggg ccgggcgccg agcgcgatca tccggcgggc gtcctcctgc
     2881 gactgcatcg cgaagacact cacgtcgtcg aggaccaccc tcagcagggg tctcgcgagg
     2941 cggtagcggg cgaacgaacg gtcggagagc cggccgttgg cgatcatcac cggaacgcgc
     3001 tggcgcgcgg cgtggcgcag cacgttgggc cagatctcgg tctccatgca gatcaagaag
     3061 gccggatgga tggctcggaa ggcccgcctc acggcgccag ggaggtccac cggaaagaag
     3121 cggtgagcgg cgacgccggc gtaccggtcg cgtactacgc gcgcgccggt gtccgtcacc
     3181 gtcgtcacga cgagggccag cgccgggtac gcgcggcgaa ggccgtccag aagcgcctgg
     3241 gcggcgatcg cctcaccgac cgagaccgcg tgcacccagg cggcgggccc ggtcaccggc
     3301 cgcgcgccga agcccagccg tgccttgagg tgaagcggta cgccgtgcgc ggcgcggcgc
     3361 cagagcgcga ccggcaggta tcccaccgag agcgcggcca gcaccagcgt gtagacgccg
     3421 tggatcacga cgccaccagg cggtcggcgg ctgcggtcgt ctcgtcgagc gcctgcgcga
     3481 tgtccttcgc cgcgcgctcc cgcccggcgt ccgccggcac gcagatcggc tgcccgagca
     3541 cgagggcgca gcgcgcgaag ggcagcggca cgaggaacgc gtcccacgag gccagacgcc
     3601 gcgccgggcg agtggcgatc gccagcggca ccacgggcgc gccggtcagc gcggccagga
     3661 cgaccacgcc ctccttcagc cggccccgcg gcccgcgcgg tccgtcgggc acgagcgcga
     3721 catcctcgcc gttccgcacg gcgtcgacga gcgcgcgcag cccgccggcg ccgccgcgcg
     3781 acgacgagcc ccgtacggcg gccactccga accgtgccgc gtagcgcgcg accagctcgc
     3841 cgtcccgcga tcgactcgcg aggaccgaaa cgcgccgcgc gccgtgcgtg cgacggagcc
     3901 gcgcgctcgc ccacggcatc atcaagaccc gcccgtgcca cgccgcgtaa atcaccggcc
     3961 gccccgcctc ccacagcggc cgcacccgct ccgccccgac gacctgcaac cgtagtgtcc
     4021 tgccgagcaa gcgcacgacg ctcgcggcca gcatggcgat cagctgcgcc ttcacgaccg
     4081 ggccacgttc gagcgagggc tttgcccgcg cgacgcgtgc tgggggagac ctcggaggga
     4141 gccgtcgaag actccaccga tcgacagtac atgtcgcgcc gcccgcgcgc ccacgcccgg
     4201 ctcgccgcaa agactccgga gctcgacgaa agccgcgcgc tgcgcgtcga gttcacctgg
     4261 cgtgtcgagg agacgcaggg ccgccgacgc gagccgctcg ggcgtcgcgt ggcgcctctc
     4321 gtagagctcg ggtacggcct cgcggccgag cgtcaggttc gtgagcccga tccacggcac
     4381 gcgcaggagc acgcgaccca gcagctcggt ggctgccgac acgcggtagc agacgatcag
     4441 cggcgtgccg agcagcgcgg cctcgagcgt caccgtcccc gacgtggcca ggaggagatc
     4501 cgccgcgcgg atcaccgcgt ggctgtcgtc ggcgacgatg cgcacgggcg gcccgcccgc
     4561 gagcgcgcct cgcacgggcc ccgcgcccac cgtcgacgcc aggccgagcg cgaacgtcgc
     4621 ctcgggatgc gcggcggcga tgcgccgggc ggcctcccgc atgagcgggg ccatgcgccc
     4681 cacctcctcc gcgcggctgc ccggcaggag accgatcagg agtgcgcccg acgggacgcc
     4741 gagacgctcg cgcgcctcgg cgcgcgacgg cgcatggccg agcgcgtcga ggaccgggtg
     4801 gcccacgaac tcgacgggta cgcctgcgcg ccgatagatc gccggctcga acggcagggc
     4861 agcaaggacg agcgataccc agcggcggat ggcgcggacg cgccacgcgc gccaggccca
     4921 gatttgcggg ggaacgaagt agaccacggg cacacccgcc cggtgcgccg cccgcgccag
     4981 tcggaggttg aactccggga agtcgatcag caccaggaca gcggggcgct cccgctcgag
     5041 acggtcgcgg agcacgcgga acgtgcgcca gagcgccgga atccgcccga gcgcctccgt
     5101 ggtgccgacg gcggcccgcc gggtgacgtc ggccacgaga tccaccccct cggccgccat
     5161 gcgcgcgcct cccaggccga gcagcgtgag ctccggcgcc tgcgagcgga gggcccggca
     5221 gagcagcgcg ccgtggccgt caccggacgc ctcaccggcc gacagcatga tcgtcgtcgg
     5281 gcgaagcccc tcagccggtc gcatccacgc tcacgagggc gatccccagc gcatcggcgg
     5341 cccgcgcggc cgccccggga tcgacgacca gcacccggcc ggattccacg gcgatgacgg
     5401 cggcgcgccc ctcggccgct gccgcgaccg tctcggcgcc caccgccggc gtgtcgaagc
     5461 ggtaatcgtg gtcgcgcgcc accgccttga cgaccaccgc gccggggccc gcgagcagcg
     5521 tgccgcgccg gatggccgcg gtcgtaccct cgaccgcctc cacggcggtg acgacgccgt
     5581 gtcggacgac gaccgtctgt ccgatgcccg cgtcggccga caggtgcgcg acggcgagcc
     5641 ctcgccggac gtcggccacc tccgcatccg acggacgcct cgcggtcagg cagccggggg
     5701 cggcgaggtc gccgaggaac ggccgctggt ccaggacctc cacgccgatc gacgcgaaca
     5761 tcgcgacgat ggcctcgagc agcttggcgt ccatgcgcga gccggcgtgc tcgccgatcc
     5821 gctgcgacgc ttggtctgcg gacgcggcgt ccgcgcgcag cacctcgctc atcgagaagc
     5881 gtccgccgaa gagcgcggcg gtggcgcctt ccgcttgcag cgtggcgagg accgcggccg
     5941 cctcggtgat gcggctgggc acgacccgat cggcgtggca cgccatctcc tccgcgccgg
     6001 ggaaggcgaa ggccacgacg cgccagcccc ggcgccgcgc cgcctgcgcc atctgcgcgg
     6061 gcagcggtcc ggcgccgcac atcaggccga gccggcgctc cacggccagt ccggcacgaa
     6121 ctcgatctcg gggcgccgat cgcgaatgag ccgctcgatc tcgagcgcca tggccagcga
     6181 gcggacgtcc tcgacctccg ccaactccac cgcctcgccg gcggccagcc gccgcgcggt
     6241 gcggacgagg tggagtacct ccagtttgag cggattgtcc ttgtgaacga agaggtgctc
     6301 cacgaaggac gcctggcggt agcggatgga ctcgcggttg ggcagcgact cctgggcggc
     6361 gcggcggtgg atctggatgt cctgcaccag gtaatccagc acgatatagg cgtccggctg
     6421 cgtgatggcg agcgtacgga tcttctcctc ggtgacccgg ctcgccgtca gcgtcgcgat
     6481 cgtgcccgac tggaagccga gctggacgtt ggcgacgtcg gtgacgtccg agtgcacggc
     6541 ggcaccgaac gcggccaggc gctggggcgg cgacgggacg agcgccagca cgatgtcgag
     6601 gtcgtggatc atgaggtcca tgaccacggt gtccttctgc acccgggaga cgaagggtcc
     6661 gagccgccgc gactccacga ggatcggctg ctccacgatc ttgcggagct cctgcaccgc
     6721 gccgttgaag cgctcgacgt gccccacctg cagggcggcg ccggagcgcc gcgcgatggc
     6781 gaacagctcg cgcgcctcct ccaacgtcgg cgtgatcggc ttctcgacca gcacgccgat
     6841 cccggcctcg agcaggtcgc gggccacgtg aaagtgctgc tcggtcggca ccgcgacgct
     6901 gaccacgtcg accttgccga ccaggtcgcg gtggtcggcg aaggcccgcg tgtcgtagtg
     6961 ccgggcgacg gccgcggccc gtccggcgtc gatgtccacg acgcccacga ggtccacgtc
     7021 ccacagctcg gcgtagacca gcgcatggta ctggcccatg tggccgacgc ccaccacgcc
     7081 cgcgcgggtc cggtgatccc cacgggtcat cggctcacct gctcgctctc ggcttcctcg
     7141 gccagcgacg ggccgcgatc acgccagccg cgcgggggcg cgcagattcc gcgccgggtg
     7201 gaggccgcca cgaaggcgat gaggcgctcc acggcggctc cggccggcgc ctccttccgc
     7261 agacgcgcca gtgccgggcc aggcgccagc ccggcacgat agaggatccg atgagcgtcc
     7321 ttgagcgcgc ggcgctcggc cgcggacatg ccggcgcgcc gcagcccgac gttgttgatc
     7381 ccgtgcaccg tggcgggcac gccgttggcc agcatgtagg gtggcaggtc ggcctcgagc
     7441 ttcgagaagc caccgaggta accgtacgtg ccgacccggg tgaacggcgg acagccgctc
     7501 tgaccgccga tggtgacgaa gtcctccagc tcgcagtgtc ccgacagtcc cgcgtagttc
     7561 acgaggatca cgccctcgcc cagccggcag tcgtgtccga catgagtgag gcccatgagc
     7621 aggcagcggg gaccgatctc ggtccacgac tcggcgcggg tggcgcggtg gatggtcacg
     7681 tactcccgga tcaccgtgcc cgacccgacc cggacgcccg acggcgtcgc ggggtcgaac
     7741 ttgagatcct ggggcgcgcc gccgagtgcc gcgccgtgtc cgatccgcac gtcgggtccg
     7801 atcacgacgc gcccttcgag gacgacgtgg tgaccgacct cgacgccgcg cccgagcgtg
     7861 actccgggac cgaccacgca gaaggcgccg acggtggcat tctcgccgat ctcggcccgt
     7921 gggtcgacca ccgccgtcgc gtggatgcgc tcggtcacgg ccggccgccc tccttctcga
     7981 ggcgcgcttc gagatcggcg agccgacgct cgagggtccg cacccggcgg accatctccg
     8041 gcaggtcggc ctcggcgatc cagatccggc gcgcctgcac gatgggtcgc gcccaggtcc
     8101 cgaagacctt ctcgccggca gggacgtcct gggagacgcc ggtcttggcc gccaggatcg
     8161 ccccctcccc gatggtcacg tggtcggcca cccccgcctg ccccagcatg atgacgccac
     8221 gaccgatccg cgaggagccg gcgatcccgg actgggcggc cacgatgcaa tcctcaccga
     8281 tgtccacgtt gtgggccacc atcacgaggt tgtcgatctt cgtcccccgc cggacgacgg
     8341 tctcgccgag cgtggcccgg tcgatggtgg tgttggcgcc gatctcgacg tcgtcctcga
     8401 tgcgcacccc gccgacctgc gggatcttgc ggtgacgcgc gccgtcgaag gcgtacccga
     8461 agccgtcggc accgaggacg gcgcccgcat ggacgatcac gcggcggccg atccggacgc
     8521 gctcgcggac gaccacatgg gggtggagca cggagtcctc gccgatctcg gcgccggccc
     8581 ccacgtaggc gagcgcatgg acgcggacac gctccccgat gacggcgccg gcctcgacga
     8641 ccgcgaaggc gcccacggat gcagtcgccg ccacgcgagc gcccgccccg atgatcgcgg
     8701 acggggcgac gcccggcggc gccgcggccg gcgggtggaa catcgccagc agatccgcca
     8761 gcaccagccg gggggccgtg gcccggagca ctgggccggg caggcccacg aggtcgggtg
     8821 gggcgaggaa ggcccccgcc cgggaggcga gcgccgagtc gcggtggcgc gcgtcggtga
     8881 cgaaggtgat gtcgtcggga ccggcggcct cgagcgccgc cacgcccgtc accgaccgca
     8941 tggcgtcgcc ctccagcgtc agccccagcg ctgcggcgag ctcgccgagc gtgaagcctg
     9001 gcgtgtgctc catcgggtta cttcttgccc ctcgtcgcag cctcctggtc ataggcgcgg
     9061 atgatctcgt ccgtgagatc ggcctccggc gcccagtaga tgacacccgc gttccggccg
     9121 tcgaggatca gatagtaacc cttctgcttg ccgagcttgt cgatgatccc ctgcagctcc
     9181 tggcgaacct tcacgatgag ctgctgctcc ttgcgcttga gctcccgctg caggtcgtcg
     9241 gccagccgcg cggcatcacg gcgcttgcgc tcgatcgtct cctcccgttc gcgccgggca
     9301 tccggggaca tcagggcgcc tttcttctcc agctcctcgc gcagcttttc gaactcctga
     9361 cgccgggcgt ccatctcctt ctgcatggtc gctttttctt tctcgagctg ctcgcgggcg
     9421 gcgacgccgg cggcggaacg gacgagcacc cgctggatgt cgatgaatcc gatgcgtgtc
     9481 accgacggag cttgcgcgcg cgccgcgccg tcgaacgtca gcatcccggc cgccacggcg
     9541 ctcacgaaca ccaaggccgc ggtcagtgtg tgtctcatgc agctcctcct agaacggcgt
     9601 gccgacggag aagtggacgg ccccgacgtc ctcgcccgcc cggcggtcga ggttgaagcc
     9661 gtagtcgatc ctgatcggcc cgaacggcga gttccagcgt acgcccattc ccgcggcctt
     9721 gcggaggtcc gtgatgtcga agctggtggt gaacccgtac acctggccga tgtcgaagaa
     9781 ggcggccagg cggatgttga acggcagcgg cacgaggtac tcgacgttgc cgaggagctc
     9841 cgtggtgccg ccgatccgcg tgcccgtctc gtccacgggt gagagctccc ggaacttgaa
     9901 gctcctgacc gagttcgggc cgccgaggaa gaaccgctcg aacagcggca gatcctcgtc
     9961 cccccatccc agaccgtagc cgacctcggc gcgcgcggac agcacgtgcc cgaaccagat
    10021 cggcttgaag tagctcgtgt tgaagatggt cttcacgaac tgggagtcgc ctccgaaccc
    10081 cgcgacgtcc acggtcagcg tggtcagccc tccgcgggtc ggcagcagga tgctgtcgcg
    10141 gctgtcacgg gtgacgcctc ccgacaccgc ggaggtgatc cgggtcccct cctcctcgcg
    10201 cagcgcgctg ctcgcgctgg cgctcacgtc gccgatgtcg tcgcgggtga gccggtagcc
    10261 cgtggtccag cgccagtatt ccgcgaacgg gtgactcagg cggagaccgc cgccgaggga
    10321 atcgtagtcg tagtcgtcgt agacgcgcct gaggttgaag aggtcgaagc cggccgccag
    10381 cggacggtcg aagagccagg gctccgtgaa gctgatgatg ccctgctgcg tctggccgcc
    10441 gatgcggatc cgcatcgag
//
LOCUS       DATIAZ010000091         4396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_417175, whole genome shotgun sequence.
ACCESSION   DATIAZ010000091 DATIAZ010000000
VERSION     DATIAZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417175"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VLF19_03120"
     CDS             <1..291
                     /locus_tag="VLF19_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564903.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HSE92270.1"
                     /translation="LHRMPEEVVAAALQEDVDVLGISLLSGAHMTLVPRVLALLREAG
                     AADIAVVVGGVIPDEDVAALKALGVRDVVVQDTPPDEVVRRVREAAAARSGR"
     gene            288..1739
                     /locus_tag="VLF19_03125"
     CDS             288..1739
                     /locus_tag="VLF19_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE92271.1"
                     /translation="MSAGDLAAAARRRGVDLTVWPPRYDATYRPAPEAEYWLPEVECA
                     DPAARDALVLAKLRRQVEWAWQRSPFYRRKWNDAGVSPDTLVGLEDLARFPVVQKAEL
                     RAAQAAAPPFGDYLCVAPHEVARIHGTSGTTGRPTVFGVGADDWARIGEAHARVMWGA
                     GLRPDDRIMVCSFFSLYLGSWGALVGGERLGAAMFPFGAGVAGQTLMGVGWARDLRPT
                     AFYSTPSYALHFAETARREGLDPRDLGFRVMFFSGEPGAGIPSTKRLIEDTFGAACVD
                     MGSMAEMTPWMTSAECRHRTGMHLWQDLVYTQVCDPATWQPVPFGAEGTPVYTHLERT
                     SQPMIRLVSGDLARWADEPCPCGRTYPRLPAGLYGRIDDMFIVRGENIYPSAVEDTLR
                     AIEGFGGEFRVIISRREAMDELLVRAEYAANHAAPERRARLAASIAERLRARLGVRPV
                     VELVPEGDLPRTEFKARRVIDDRDLYRRTLAGS"
     gene            1855..3540
                     /locus_tag="VLF19_03130"
     CDS             1855..3540
                     /locus_tag="VLF19_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSE92272.1"
                     /translation="MFDPDVIARARRMREEWEAKELAEFLARQPESRAERRTASGLPV
                     KRVYTPEDLTAVTAEDMGLPGRYPFTRGPYPTMYRGRLWTMRQIAGYGTGSDTNRRFR
                     YLIGQGQTGLSVDFDMPTLMGYDSDDPRALGEVGREGVAVDTIEDVHALFDGIDLETI
                     SVSMTINPTAWILLAMYVGLAQERGLDLGRLSGTCQTDILKEYMAQKEWIFPIRPSLR
                     LVRDMIVFGARTLPRYNPINISGYHISEAGATSVQEVAFTMANAIAYVEEVTRAGVPV
                     DEFAPRLAFYFVAQADFFEEVAKFRAARRVWAKIMKERFGATRPESMRLRFHCQTAAM
                     TLTKAQPLNNIVRTAFQALSAVLGGAQSLHTNGLDEAYAIPSEQAMKVALRTQQILAE
                     ETRVPDVVDPLGGSYYVEALTSDLERAVFEILAKVDVLGGTVKAIEEGWFQREIAESA
                     YDDARRRASGERTVVGVNRHAEPGAPPADLEIHRVHPEVEARQITHVRQVRAGRDGAR
                     VAALLDRLEREARDPAVNLMPVTIDLVRARASVGEIVARLRLVWGTYVERPVF"
     gene            complement(3545..>4396)
                     /locus_tag="VLF19_03135"
     CDS             complement(3545..>4396)
                     /locus_tag="VLF19_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92273.1"
                     /translation="NVAVGTGETGPFLTIEQVVVARAVAGPRRTTVLSVYNLIGYAAA
                     AFGAAAAGAAGGYRPLFLLFLAGAAVQAAAYALLPASPRVEARPAGPLPSAPLIRRMA
                     ALFALDSFAGGFVLQSLVAYFLHVRFGLGLVELGLTFFVAQVVTAASLLLAAPLAARF
                     GLLRTMVVSHLASNVFLMAIAGAPSAGVAVALLLLRQLLSQMDVPTRQAYVMAVVEDH
                     EREAAASLTNLARTAAQAVTPALTGWTMQAVALSAPFVLGGGLKIVYDLALWFTLRDV
                     KLREGGR"
BASE COUNT          627 a         1573 c         1621 g          575 t
ORIGIN      
        1 ctgcaccgga tgccggagga ggtcgtcgcc gccgccctgc aggaggacgt ggacgtcctc
       61 ggcatcagcc tgctctccgg cgcgcacatg acgctggtgc cccgcgtgct cgcgctgctg
      121 cgcgaggccg gtgccgccga catcgccgtc gtcgtgggcg gcgtgatccc cgacgaggac
      181 gtcgcggcgc tcaaggcgct cggcgtccgc gacgtggtcg tgcaggacac gccgcccgac
      241 gaggtcgtgc gccgcgtgcg cgaggccgcc gcggcgcgca gcgggcgatg agcgcaggcg
      301 acctcgctgc cgccgcccgc cgccgcgggg tcgacctgac cgtctggccg ccgcgctacg
      361 acgccacgta ccggccggcg cccgaggcgg agtactggct gcccgaggtg gagtgcgccg
      421 acccggcggc ccgcgacgcg ctcgtgctcg ccaagctccg gcgccaggtc gagtgggcct
      481 ggcagcggag cccgttctat cgccgcaagt ggaacgacgc cggggtgtcg ccggacacgc
      541 tcgtgggtct cgaggacctc gcgcgctttc ccgtcgtgca gaaggccgag ctgcgtgccg
      601 cccaggccgc agcgccgccg ttcggcgact acctgtgcgt cgcgccccac gaagtcgcgc
      661 gcatccacgg cacgagcggc acgaccgggc ggccgaccgt gttcggcgtc ggcgccgacg
      721 actgggctcg catcggtgag gcgcacgccc gcgtcatgtg gggcgccggc ctccggccgg
      781 acgatcggat catggtctgc tcgttcttca gcctctacct cggctcctgg ggcgcgctcg
      841 tgggcggcga acggctcggc gcggccatgt ttcccttcgg cgccggcgtg gccggccaga
      901 cgctgatggg cgtgggctgg gcccgcgacc tccggccgac ggccttctac agcacgccct
      961 cctacgcgct ccacttcgcc gagaccgcgc gccgcgaagg gctcgaccca cgcgacctcg
     1021 gctttcgcgt catgttcttc tcgggcgagc ccggcgccgg tatcccgtcc accaagcgcc
     1081 tgatcgagga caccttcggc gcggcgtgcg tggacatggg aagcatggcc gagatgacgc
     1141 cgtggatgac cagcgccgag tgccgccatc gcaccggcat gcacctgtgg caggacctcg
     1201 tctacaccca ggtgtgcgat ccggcgacgt ggcagccggt gccgttcggc gccgagggca
     1261 cgcccgtcta cacgcacctc gagcggacgt cgcagccgat gatccggctg gtctccgggg
     1321 acctcgcccg ctgggcggac gagccgtgtc cctgcggccg cacgtatccg cggctgccgg
     1381 ccgggctcta cggccgcatc gacgacatgt tcatcgtgcg cggcgagaac atctacccga
     1441 gcgcggtcga ggacacgctg cgcgcgatcg aggggttcgg cggggagttc cgggtgatca
     1501 tctcccgccg ggaggcgatg gacgagctgc tcgtgcgcgc cgagtacgcc gcgaatcacg
     1561 cggctccgga gcggcgcgcc cggctggccg cgtcgatcgc cgagcgcctg cgcgcgcggc
     1621 tgggtgtgcg cccggtcgtg gagctggtgc ccgagggcga cctcccgcgc acggagttca
     1681 aggcccggcg agtcatcgac gaccgcgatc tctaccgacg aacgctggcc ggctcgtagg
     1741 ccgagcgtcg ggtcgccgtg cgaggtgccg gccggcccgg ccgtccagcc gtgcggtgaa
     1801 atggccgtcg gccctgcgca ggcgccccga cggcgagacc aggagtcatg cgccatgttc
     1861 gaccccgacg tcatcgcccg cgcgcggcgg atgcgcgagg agtgggaggc gaaggagctg
     1921 gccgagttcc tggcccgtca gccggagtcg cgcgccgagc gccggacggc ctcgggcctg
     1981 ccggtgaagc gcgtgtacac gcccgaggac ctcaccgccg tgacggcgga ggacatgggc
     2041 ctgcccgggc gctacccctt cacgcgtggg ccctacccga ccatgtaccg cggccggctg
     2101 tggacgatgc gccagatcgc cggctacggc acgggcagcg acaccaaccg gcgcttccgc
     2161 tacctcatcg ggcagggcca gaccggtctc agcgtggact tcgacatgcc gaccctgatg
     2221 ggttacgact ccgacgatcc gcgcgcgctg ggcgaggtcg ggcgagaggg cgtggccgtc
     2281 gacaccatcg aggacgtgca cgcgctcttc gacggcatcg acctcgagac gatctcggtg
     2341 tcgatgacca tcaacccgac cgcctggatc ctgctcgcca tgtacgtagg acttgcgcag
     2401 gagcgcgggc tcgacctcgg ccggctctcc ggcacctgcc agaccgacat cctcaaggag
     2461 tacatggcgc agaaggagtg gatcttcccg atccggccct cgctgcgcct cgtgcgcgac
     2521 atgatcgtct tcggcgcccg caccctgccg cgctacaacc ccatcaacat cagcggctac
     2581 cacatctccg aggccggggc gacgtccgtg caggaggtgg ccttcacgat ggccaacgcg
     2641 atcgcctacg tggaggaggt cacgcgcgcc ggggtgccgg tcgacgagtt cgcgccgcgc
     2701 ctggcgttct acttcgtggc gcaggccgac ttcttcgagg aggtcgcgaa gttccgcgcc
     2761 gcccgccgcg tgtgggccaa gatcatgaag gagcgcttcg gcgccacccg gccggagtcg
     2821 atgcggctgc gcttccactg ccagacggcg gcgatgacgc tgacgaaggc gcagccactg
     2881 aacaacatcg tgcggacggc cttccaggcg ctctcggcgg tgctgggcgg cgcccagtcg
     2941 ctgcacacca atggcctgga cgaggcgtac gccatcccgt cggagcaggc catgaaggtg
     3001 gcgctccgca cccagcagat cctcgccgag gagacccgcg tgcccgacgt cgtggacccg
     3061 ctcggtggct cctactacgt cgaggcgctc accagcgacc tcgagcgtgc ggtgttcgag
     3121 atcctggcga aggtcgacgt cctggggggc accgtgaagg cgatcgagga gggctggttc
     3181 cagcgggaga ttgccgagtc ggcctacgac gacgcgcggc gccgggcctc cggcgagcgg
     3241 acggtggtcg gcgtcaaccg tcacgcggag cccggggcgc cgccggcgga cctcgagatc
     3301 caccgggtgc atcccgaggt ggaggcgcgt cagataacgc atgtgcgcca ggtgcgggca
     3361 gggcgggacg gcgcccgggt ggcggcgctg ctcgaccggc tggagcgcga ggcccgcgat
     3421 ccggccgtga acctgatgcc ggtgacgatc gacctggtcc gcgcgcgcgc atcagtggga
     3481 gagatcgtcg cgcggctgcg cctcgtctgg ggcacctacg tggaacggcc ggtgttctag
     3541 ggcttcaccg gcctccctcc cgcagcttca cgtcgcgaag ggtgaaccag agcgccaggt
     3601 cgtagacgat cttgaggcca ccgccgagga cgaacggcgc cgacagcgcg acggcctgca
     3661 tcgtccagcc ggtgagcgcg ggggtcaccg cctgggccgc cgtgcgagcg aggttcgtca
     3721 ggctcgcggc ggcctcgcgc tcgtggtcct cgacgacggc catcacgtag gcctgccgcg
     3781 tgggcacgtc catctgcgag aggagctggc ggagcagcaa cagcgccacc gccaccccgg
     3841 cgctcggggc gccggcgatc gccatcagaa agacgttcga ggcgaggtgc gacaccacca
     3901 tggtgcggag caggccgaag cgcgcggcga gcggggcggc cagcagcagc gaggccgccg
     3961 tgacgacctg ggcgacgaag aaggtcagcc cgagctcgac cagccccagc ccgaaccgga
     4021 cgtgcaggaa gtaggcgacc agcgattgca gcacgaagcc gcccgcgaag gagtcgagcg
     4081 cgaagagcgc cgccatgcgg cggatcaacg gcgccgacgg gagcggtccg gccggccgcg
     4141 cctccacacg cggcgaggcg ggcaggagcg cataggcagc ggcctgcacg gccgcaccgg
     4201 cgaggaacag gaggaacagc ggccggtatc cgccggcggc tccggccgcg gccgccccga
     4261 aggccgcggc cgcgtacccg atcaggttgt agacgctcag caccgtcgtc cggcgcgggc
     4321 ccgcgaccgc gcgggcgacg acgacctgtt cgatcgtcag gaacgggccg gtctccccgg
     4381 tgccaacggc gacgtt
//
LOCUS       DATIAZ010000092         7162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_417354, whole genome shotgun sequence.
ACCESSION   DATIAZ010000092 DATIAZ010000000
VERSION     DATIAZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417354"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VLF19_03140"
     CDS             complement(<1..236)
                     /locus_tag="VLF19_03140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE92274.1"
                     /translation="MSSKFWRLLVAEPLHRHTVLDVGTGSGRLALALAPQCRSALGID
                     RDPAVIEEARRRARTAGLRNVEFLVADAERIEYD"
     gene            complement(455..2128)
                     /gene="ilvD"
                     /locus_tag="VLF19_03145"
     CDS             complement(455..2128)
                     /gene="ilvD"
                     /locus_tag="VLF19_03145"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSE92275.1"
                     /translation="MAFDPRHKSHVLLDGTDRAAARSYFRAVGFSDEDLARPLVGVAH
                     CWIELTPCNSNHRKMAEKVKEGVRASGGTPIEFNTISVTDGISMGTEGMKASLISRDW
                     IADSVELVVRGHLLDGFVGISGCDKTIPAMVMAMARLNVPSVMLYGGSIMYGEYRGRR
                     LTVQDVFEAIGARNAGKIGEEELHAIECAACPGDGACGGQFTANTMATAFEMLGVSPM
                     GFNDVPATHGDRPEIAFQTGRLAMDVLRRGLLPRQIITRKSLANAIAGVMATGGSTNA
                     VLHLLAIAKEAGVRLTIDEFDRISRRTPLLADMKPWGAYTAPEMYEAGGMGVVASRLL
                     DAGLLHADEITVTGRTIGEEARAAREAPGQKVIRPLASPLKGYGGIVILRGNLAPEGC
                     VAKVSGQKRDLHRGPARVFDREEDAFAAVKAGKVKANDVIVIRYEGPKGGPGMREMLH
                     VTGAIQGAGLGDSVALMTDGRFSGATHGFMIGHVAPEAADGGPVAALRSGDTIVIDIK
                     KRRLDVELSAAELKKRLRAVKPPKPRYTWGVMAKYARLVSSASDGAVTG"
     gene            complement(2139..2549)
                     /locus_tag="VLF19_03150"
     CDS             complement(2139..2549)
                     /locus_tag="VLF19_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92276.1"
                     /translation="MPAEQTYFEDVEAGEHTETPAITLTQAHVSLYRGLAGEAADDPA
                     AVPSLLPLCLTTGLGWRVPRAPLAVQAFLSVEWEMLKPLTVGDTIATRARAVQTRSMR
                     DGGLVIEAYEVVDQRGEVVQRGRVTFLVAKRPTP"
     gene            complement(2539..2988)
                     /locus_tag="VLF19_03155"
     CDS             complement(2539..2988)
                     /locus_tag="VLF19_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HSE92277.1"
                     /translation="MTAHRRYFDDIQVGEEYESPGRTVTETDIVLFAGLSGDYNVLHT
                     DAEFMKTSIFGERIAHGLLGLAIQAGLFTRATPAYATLAFEGLRWKFKGPIKIGDTIR
                     LRARVIAKTESAKPDRGVVIVARTVVNQRDEVVQEGETELMVARRAG"
     gene            3145..3663
                     /locus_tag="VLF19_03160"
     CDS             3145..3663
                     /locus_tag="VLF19_03160"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HSE92278.1"
                     /translation="MGFLEALSRVVDAINERVGRSIRVLTLLMVVITTYDVVMRYVFR
                     TSFVFIQELEWHLFAFLFLVAAGYAHLKGDHVRVDIFYARMAPRARAAVDVGGALLFL
                     FPTCFLVVVTAIPFVRASLRVLEGSPDPGGIPARYILKMAIPAGFGLLALQGISQLVK
                     SVRTLQGKGVAP"
     gene            3660..4979
                     /locus_tag="VLF19_03165"
     CDS             3660..4979
                     /locus_tag="VLF19_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSE92279.1"
                     /translation="MSEFLPLLMFAVFTVLILAGYPIAFTIGGTALFFGLYAFGLDFF
                     NLLPLRIFGVMTNFTLIAVPLFIFMGVTLERSGLAEDLLETMALVFGRLPGGIAISVV
                     LVGTLLAATTGIVGATVTTMGLISLPTMVRRGYQKELATGIICASGTLGQIIPPSIVL
                     VLVGDILGVSVGDLFLGAAIPGLILSGLYIVYVLAVAALRPALVPAIPAAERAQVSRQ
                     EMTRRVTRVLIPPLALIMAVLGSIFFGIATPTEAAAVGASGAMGLTLLNRRFSSTMLK
                     ETMDVTTRLTSMVFIILVGATAFGLAFRGMQGDRLIEKYVAESGLPPLAVFWLVMTVV
                     FVLGFFLDYIEIAFIHLPVISPVLLSMGFDPLWYGVLLGVNLQTSFLTPPFGFALFYL
                     RGVAPPEVTTGHIYRGIIPFVILQIVGLAAIALFPATATWLPRFVFK"
     gene            complement(5040..6134)
                     /locus_tag="VLF19_03170"
     CDS             complement(5040..6134)
                     /locus_tag="VLF19_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92280.1"
                     /translation="MIRRTFLAKTGGALAAAGAAVAVDAPDVIAQPKFQWRMVTTWPK
                     GLATLHGAAVRFAEMVDRMSAGRLKIQVFAGGELVPPLQSFDAVTQGTVQMAHSAAYY
                     WAGKTAACQWFGAVPFGLSAQGMNAWFYEGGGLKLWEEVYAPFGLVARPCGNTGTQMA
                     GWFKKKINSIDDFKGLKMRIPGLGGKVVAKAGGTVVLLAGGEIFPALERGVIDATEWV
                     GPEDDLKLGLHNAARYNYYPGWHEPGTVEEAFFAKKAYDGLPAELRAIVDHACQANNV
                     HVLADYNTRNAQGLQKILAEMKGKVEVLQLPDSVLKALKPLAEATLEEEAAKDPMARK
                     VHEGFKKFRKLYNDWDKVSEDPYQRLIASA"
     gene            6361..>7162
                     /locus_tag="VLF19_03175"
     CDS             6361..>7162
                     /locus_tag="VLF19_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSE92281.1"
                     /translation="MRFVDRRLEPVWDKVRARERLDRTDGLALFETDDLLGVGRMAGH
                     VKSEREGERVYFVINRHITPTNVCVLSCAFCDFARKQGAEGAYEHTIEDMVGMVREGV
                     REVHIVGGHHPEWPFEYYERVIGAIHAAHPEVQIKAFTAAEIDYFWRRWKIEPQEALA
                     RLKAAGLQSMPGGGAEIFSARLARALRYAGKADPERWCEIHGIAHALGIRTNATMLYG
                     HVETMAERVDHLLRLRDQQDASGGFLTFVPLAYQVGTTRLRPRQTPPTD"
BASE COUNT          980 a         2575 c         2382 g         1225 t
ORIGIN      
        1 tcgtcgtact cgatccgctc ggcgtccgcg accaggaact cgacgttacg aaggccggcg
       61 gtccgggccc gacgccgcgc ctcttcgatc accgccgggt ctcgatcgat gccgagcgcc
      121 gagcggcatt gaggcgcgag cgccagcgcg agacgccccg agcccgtccc cacgtcgagc
      181 acggtgtggc ggtggagagg ctccgcgacg agcagccgcc agaacttcga gctcacgccg
      241 gccggagcac tatccgcgca cgggatcatt gtatctgtcg tcggtgtgcc ggcgccggcg
      301 ccgaaacgac ctttgccttc gctcgtttgc ccctcggctc ggccggcgag ccggcgtgcg
      361 cctcgcacct caacggccgg cggatcctcc cggggactgc cggtctgccg tcggggcgcc
      421 tgcggaggcc ctccggcgcc cgacggtcgg cacctcaccc cgtcacggca ccgtctgacg
      481 cggacgagac gagccgagcg tacttggcca tgacacccca cgtgtagcgc ggcttcggcg
      541 gcttcacggc acgcaggcgc ttcttgagct ccgccgccga cagctcgacg tcgagtcggc
      601 gcttcttgat gtcgatgacg atggtgtcgc cggaccgcag cgcggcgacc ggtccgccgt
      661 cggcggcttc cggcgcgacg tggccgatca tgaagccgtg cgtcgcgccc gagaagcggc
      721 cgtcggtcat cagcgcgacg ctgtcgccca gcccggcgcc ctggatggcg cccgtcacgt
      781 gcagcatctc gcgcatccca ggaccgccct tgggcccctc gtagcggatc acgatcacgt
      841 cgttggcctt gaccttcccg gccttgaccg ccgcgaacgc atcctcttcc cggtcgaaca
      901 cgcgcgcggg gccgcggtgc aggtctctct tctggccgga aaccttcgcg acgcatccct
      961 cgggcgcgag gttgccgcga aggatcacga tgcccccgta gcccttgagg gggctggcga
     1021 gcgggcggat gaccttttgg cccggcgcct cgcgcgccgc ccgcgcctcc tcgccgatgg
     1081 tgcgtccggt caccgtgatc tcgtccgcgt ggagcagccc ggcatcgagc aatcggctgg
     1141 ccaccacgcc catgccgccc gcctcgtaca tctcgggagc cgtgtaggcg ccccacggct
     1201 tcatgtccgc gagcagcggc gtcctgcgcg agatgcggtc gaactcgtcg atggtgagcc
     1261 gaacccccgc ctccttggcg atggcgagca ggtgcaggac cgcgttcgtg gaccctccgg
     1321 tggccatgac gccggcgatg gcgttggcga gagacttacg ggtgatgatc tgtcgcggca
     1381 gcagcccccg gcgcaggacg tccatcgcca ggcgccccgt ctggaatgcg atctccggcc
     1441 ggtcgccgtg cgtggccggg acgtcgttga accccatcgg ggagacgccg agcatctcga
     1501 acgccgtcgc catcgtgttg gcggtgaact ggccgccgca ggcgccgtcg cccgggcatg
     1561 ccgcgcactc gatcgcgtgc agctcctcct cgccgatctt cccggcgttc cgggcgccga
     1621 tggcctcgaa gacgtcctgc accgtcaggc gtcggccgcg gtactcgccg tacatgatcg
     1681 agccaccgta gagcatgacg ctcggcacgt tcagccgcgc catcgccatc accatggccg
     1741 ggatggtctt gtcgcacccg gagatgccga cgaagccgtc gagaagatgg ccgcgcacga
     1801 ccagctccac cgagtcggcg atccagtcgc ggctgatcag cgaggccttc atgccctcgg
     1861 tgcccatcga gatgccgtcg gtgaccgaga tcgtgttgaa ctcgatcggc gtgccgccgg
     1921 acgcgcggac gccctccttc accttctcgg ccatcttccg gtggttcgag ttgcacggcg
     1981 tcagctcgat ccagcagtgg gcgacgccca ccagcgggcg agcgaggtcc tcgtcgctga
     2041 accccaccgc ccggaagtac gagcgcgcgg cggcgcggtc ggtgccgtcg aggagcacgt
     2101 gactcttgtg acgcggatcg aacgccatgg tgactctcct atggggtcgg acgcttggcg
     2161 acgaggaacg tgacgcgccc ccgctggacc acctcgcctc gctggtcgac cacctcgtag
     2221 gcctcgatga cgaggccgcc gtcgcgcatg gaccgcgtct gcacggcccg cgcgcgggtc
     2281 gcgatcgtgt cgcccacggt gagcggcttc agcatctccc actcgacact gaggaacgcc
     2341 tgcaccgcca gcggcgcccg gggcacgcgc cagccgagtc cggtggtgag gcagagcggc
     2401 aacagcgagg gcaccgcggc gggatcgtcc gcggcctcgc ccgccaggcc ccggtagagg
     2461 ctcacgtggg cctgggtgag ggtgatcgcc ggggtctcgg tgtgctcgcc ggcctcgacg
     2521 tcctcgaagt aggtctgttc agccggcacg gcgcgccacc atcagttcgg tctcgccttc
     2581 ctggaccacc tcgtcccgct ggttcaccac cgtgcgcgcg acgatgacga cgccgcggtc
     2641 gggcttcgcg ctctccgtct tggcgatgac cctggcccgc agccggatcg tgtcgccgat
     2701 cttgatcggc cccttgaact tccagcgcag gccctcgaac gccagggtgg cataggccgg
     2761 ggtcgcgcgg gtgaagagcc cggcctggat ggcgagcccg agcaggccgt gggcgatccg
     2821 ctcgccgaag atcgacgtct tcatgaactc ggcgtcggtg tggagcacgt tgtagtcgcc
     2881 ggagaggccg gcgaagagca cgatgtcggt ctcggtcacc gtgcgcccgg gcgactcgta
     2941 ctcttcgccc acctggatgt cgtcgaagta ccgcctgtgg gccgtcatgc gcgcgccagg
     3001 ataccatgcg cccggcggcg ccgtttcggg ctggcggcgg cccgagtccg cgggcgcgcc
     3061 ttgacgcgcc gccggtccat cagtagagtc ccccggagtc cgcgcggacg gatcgagttc
     3121 gcgcgggacg agatcggagg ggcaatgggc ttcctcgaag ccctctcgcg ggtcgtggac
     3181 gcgatcaacg agagggtcgg ccgctccatc cgcgtgctca cgctcctcat ggtcgtgatc
     3241 accacctacg acgtcgtgat gcgctacgtc ttccggacgt ccttcgtctt catccaggag
     3301 ctggaatggc atctcttcgc tttcctcttc ctggtggctg ccggctacgc ccacctgaag
     3361 ggtgaccacg tgagggtgga catcttctat gcccggatgg cgccgcgggc gcgggccgcc
     3421 gtggacgtcg gcggcgccct gttgttcctg ttccccacct gcttcctcgt ggtcgtcacg
     3481 gccatcccgt tcgtgcgcgc ctccctcagg gttctggagg gctcgcccga tcccggcggc
     3541 atccccgccc gctacatcct caagatggcc attcccgccg gcttcgggct cctcgcgctg
     3601 cagggcatct cccagctcgt gaagagcgtg aggacgctcc agggcaaggg cgtcgcgccg
     3661 tgagcgagtt cctcccgctc ctgatgttcg cggtgttcac ggtcctgatc ctggccggat
     3721 acccgatcgc cttcaccatc ggcggcaccg cgctcttctt cggtctctac gccttcggcc
     3781 tcgacttctt caacctcctg ccgctccgga tcttcggcgt gatgaccaac ttcacgctga
     3841 tcgccgtgcc gttgttcatc ttcatgggcg tgaccctcga gcggtccggg ctcgccgagg
     3901 acctcctcga gaccatggcc ctggtcttcg gccgtctccc ggggggaatc gccatctcgg
     3961 tggtgctcgt gggcacgctg ctggcggcga cgaccgggat cgtgggcgcc accgtgacca
     4021 ccatgggcct catctcgctg ccgaccatgg ttcgccgggg ctaccagaag gagttggcga
     4081 cggggatcat ctgcgcctcg ggcacgctgg gccagatcat ccctccgagc atcgtgctcg
     4141 tgctggtggg cgacatcctg ggtgtgtcgg tgggagacct gttcctcggc gcggcgatcc
     4201 cggggctcat cctctcggga ctctacatcg tgtacgtgct cgcggtggcc gcgctccggc
     4261 cggcgctggt gcccgccatc cccgccgccg agcgagcaca ggtctcgagg caggagatga
     4321 cccgccgggt cacgcgcgtt ctgatcccgc cgctcgccct gatcatggcg gtgctggggt
     4381 ccatcttctt cggcatcgcc accccgaccg aggcggccgc cgtcggagcc tcgggcgcca
     4441 tgggcctcac gctcctcaac aggcgcttca gcagcacgat gctcaaggag accatggacg
     4501 tgaccacccg gctcaccagc atggtcttca tcatcctggt cggggcgacg gccttcgggc
     4561 tcgccttccg cgggatgcag ggcgatcggc tgatcgagaa gtacgtggcc gagtcggggc
     4621 tgccgccgct cgcggtgttc tggctcgtga tgacggtggt cttcgtcctg ggatttttcc
     4681 tggactacat cgagatcgcg ttcatccacc tgcccgtgat ctcgcccgtg ctgctcagca
     4741 tgggcttcga tccgctctgg tacggggtgc tcctgggcgt caacctccag acgtccttcc
     4801 tgacgccgcc attcggattc gcgctcttct acctgcgcgg ggtcgcgccg cccgaggtca
     4861 cgaccgggca catctaccgg ggcatcatcc ccttcgtcat cctgcagatc gtggggcttg
     4921 ccgccatcgc cctcttcccg gccacggcga cctggctgcc ccgcttcgtc ttcaagtagg
     4981 gcgccgaccg ccgagctgac cggggcggag gccgatggcc tccgcccggc ggacgctcac
     5041 tatgcgctgg cgatcaagcg ctggtaagga tcctcgctca ccttgtccca gtcgttgtag
     5101 agcttgcgga acttcttgaa tccttcgtgg accttgcggg ccatcgggtc cttcgccgcc
     5161 tcctcttcga gcgtggcctc ggcaagcggc ttgagcgcct tgagcaccga atcggggagc
     5221 tgcaggacct ccaccttgcc cttcatctcg gccaggatct tctgcagccc ctgggcgttc
     5281 ctggtgttgt agtcggccag cacgtggacg ttgttggcct ggcaggcgtg atccacgatc
     5341 gcccgcagct cggcgggcag cccgtcgtac gccttcttgg cgaagaaggc ctcctcgacg
     5401 gtgcccggct cgtgccatcc gggatagtag ttgtagcggg cggcgttgtg gagtccgagc
     5461 ttcagatcgt cctcggggcc cacccactcc gtggcgtcga tgacaccgcg ctcgagcgcg
     5521 ggaaagatct cgccccccgc gagcagcacc accgtgccac cggccttggc caccaccttg
     5581 ccccccaggc cggggatgcg catcttgaga cccttgaagt cgtcgatcga gttgatcttc
     5641 ttcttgaacc agcccgccat ctgggttccg gtgttcccgc agggacgggc gacgagtccg
     5701 aacggcgcgt atacctcttc ccagagcttg agtcctccgc cctcgtagaa ccaggcgttc
     5761 atgccctggg cgctgaggcc gaaggggacg gcgccgaacc actggcaggc ggcggtcttg
     5821 cccgcccagt agtaggccgc cgagtgggcc atctggaccg ttccctgagt gaccgcgtcg
     5881 aacgactgca gcggcggcac gagctcgcca ccggcgaaga cctggatctt gagccgcccg
     5941 gcgctcatcc tgtccaccat ctcggcgaag cgaacggccg ctccgtgcag cgtggccagc
     6001 cccttcggcc acgtcgtgac catccgccac tggaacttgg gctgggcgat cacgtcagga
     6061 gcgtccacgg cgaccgcggc cccggcggcc gccagcgcac cgcccgtctt tgcgaggaac
     6121 gtccttcgga tcatggccag cctcctctgt gtgcgagagc gcggccggag aatagcatcc
     6181 gggccccgtg gaggacaacg agccggcgcc gtcgggatgg gctcgagctc ctccggcgac
     6241 ccgcatggtt gcttgacacc cgccgcgacg cgtaattaga gtgcatcgac cgatcggtcc
     6301 gaccccgccg gggtccggcc gagaggtgag cgatccgggt tccgccaggg aggctgtcac
     6361 gtgagattcg tggaccggcg gctcgagccc gtctgggaca aggtccgtgc gcgcgagcgc
     6421 ctggaccgca ccgacggcct cgcgctcttc gagaccgacg acctcctcgg cgtgggacgc
     6481 atggccggcc acgtgaagtc cgagcgcgag ggcgagcgcg tctacttcgt catcaaccgc
     6541 cacatcaccc cgaccaacgt ctgcgtgctc tcgtgcgcct tctgcgactt cgcccgcaag
     6601 cagggcgccg agggcgcgta cgagcacacg atcgaggaca tggtcggcat ggtgcgcgag
     6661 ggcgtgcgcg aggtccacat cgtgggcggt caccaccccg agtggccctt cgagtattac
     6721 gagcgcgtca tcggcgccat ccacgccgcg caccccgagg tccagatcaa ggccttcacc
     6781 gcggccgaga tcgactactt ctggcgccga tggaagatcg agccgcagga ggcactggcg
     6841 cgactgaagg cggccggcct gcagtcgatg cccggtggcg gcgccgagat cttctcggct
     6901 cgcctggcgc gcgcgctgcg ctacgcgggc aaggccgacc ccgaacggtg gtgcgagatc
     6961 cacggcatcg cacacgccct gggcatccgc acgaacgcca ccatgctcta cggtcacgtc
     7021 gagacgatgg ccgagcgcgt ggaccatctg ctgcgcctgc gcgaccagca ggacgcctcc
     7081 ggcggcttcc tgacgttcgt ccccctcgcc taccaggtcg gcacgacgag gctgcgcccg
     7141 cggcagaccc cgccgaccga ca
//
LOCUS       DATIAZ010000093        11533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_549672, whole genome shotgun sequence.
ACCESSION   DATIAZ010000093 DATIAZ010000000
VERSION     DATIAZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549672"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1090)
                     /locus_tag="VLF19_03180"
     CDS             complement(104..1090)
                     /locus_tag="VLF19_03180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSE92282.1"
                     /translation="MQQRTLGHSTLRVSAIGLGLMTMSGVYGKGDDEQSIGVIQRALD
                     LGVNFFDSSDMYGWGHNEELLGRALRGRRDRAVVTTKFGQVKNPSGGGNLVNGRPEYV
                     IQACDASLKRLGMDVIDLYYQHRVDPGVAIEETVGGMKRLVEQGKVRFLGLCEARPET
                     IRRAHAVHPISAVQTEYSLLYRTEAEETRETTRALGISFIAYSPLGRSLLTGQVHKVA
                     DIPEGDRRREHPRFKEENLAKNVELVKRIEAIAAEKRCTPAQLVLAWLLAQGDDVVAI
                     PGTKRKDRLEENVNSMKVKLTPEDVRRISEAVPAGAAAGTRYPEAQLKAVYI"
     gene            complement(1107..2126)
                     /locus_tag="VLF19_03185"
     CDS             complement(1107..2126)
                     /locus_tag="VLF19_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE92283.1"
                     /translation="MIRRLALAAVVATFSLVTAASAGGGFTPDPVDLPAARKEGSVTW
                     YTSTPVATAQKIATLFQQETGIKVELFRSGGSAVLRRFMQETDARRMNADVLTISDPA
                     AASALIKRDLLLPFRPKNFDKVPEAVKDPRGYHIAQRLNLVGIVVRTDQVPEPPRTWT
                     DLTDPKWKGKLVMPDPSFTAIQLMVVGTLSQKYGWDFYKKLRANDIMIVQSHQQVSDT
                     LTRGERTVAAEGLDSYTWDARKAGHKVQTLFPADGAFAIAAPTMVIKGSPHPNAARAL
                     AEFMVGDTVQKLFPTEGIYAARSDVPPPPGNPPLASIKLMPVDYDHVEKEAKNLKNRF
                     NEIYQ"
     gene            complement(2123..3058)
                     /locus_tag="VLF19_03190"
     CDS             complement(2123..3058)
                     /locus_tag="VLF19_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE92284.1"
                     /translation="MTAEPSIAGQEHWTTKGDVRLFLWEKRAGASPGRLGTILFVHGS
                     SMASTPTFDLHVPGRADSSTMDYFVRLGYDTWCVDMEGYGRSDKKRAINSDIATGADD
                     LESATEYITRTRGVRELLVYGISSGALRAALFAQRRPERVRRLALDAFVWTGKGSPTL
                     EQRRKRLAEYQTRHRRPIDRAFVRSIFERDHPGTADDEVIEAFADAILALDDSVPTGT
                     YVDMCANLPVVEPAKITVPTILMRGQYDGIASFEDLVEFFERLPNPDKQFAVMPGIAH
                     ASLQQKNYRIVRHILHGFFSQPDPVYRDAHDGRRA"
     gene            complement(3092..4150)
                     /locus_tag="VLF19_03195"
     CDS             complement(3092..4150)
                     /locus_tag="VLF19_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92285.1"
                     /translation="MAGIVLDGLSKRFGAVAAVREITLEVADREFVTLLGPSGCGKTT
                     LLRLVAGFVAPDAGAIRVGGVTLSTPERVVPPEQRGMGMVFQNYAVWPHKTVYENVAF
                     GLEVRKVARAEARQRVARVLELVNLAGLEARYPNQLSGGQQQRVALARSLVVEPSILL
                     LDEPLSNLDAKLRERMRWELKELQRRTGITFVYVTHDQAEAMALSDRIAVMAEGRVLQ
                     YGSPRDVYTRPASRAVADFMGLVNIVPGRVVRAGGDGGLVTLAGEHTVRVPLPPQAGD
                     GQAVQVAVRPESLTLTSEGIPGKVAEATYLGNLTDCHVTLRDGTRVRVQAGPGEALAV
                     GQCVHVRVDGDACTVFLG"
     gene            complement(4150..5859)
                     /locus_tag="VLF19_03200"
     CDS             complement(4150..5859)
                     /locus_tag="VLF19_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSE92286.1"
                     /translation="MSVVTRTAAPASVLRAEHVVMACAVAALAVLVILPLGSLVWGSV
                     SEDGTLTLGHFREAFANRLYLTALINSLVLGTWTAAISVAVGLPMAWAVSRTNLPGKR
                     FVHLTAVVAYITPPYLTAIAFVNLFGPNAGIVNRFLRDVLGAPAVTFNIFSMAGLVLV
                     TVPHTFPFVYLLAASALESVDASMEESAQMLGASRWRTVGVITAPLVAPAVLSGALIA
                     FVNAIALFGSQAIVGLPGRVFTLPTRIYALFDYPPQYGLASAMSLIFVAITVAALALQ
                     RRYLARRSYVTLGGKGSRPQLVDLGPARWAVLGFCVAVFVVAVLAPYLTLLAVSLSKS
                     WGLAFWQNLTLKNYRFVLFEYDVTRRAIANSLLLASAAATLAVLLGSVVAWLDLRTRL
                     PGRKVMDYASLVPLGLPGIVVAVALIQFWLRMPVPIYGTLLIILLAYTGRYVPLAVRS
                     ASASFRQIDPTLEETARITGAGWLRTFRSVSLPLARPGLFAGWLLVFVPAIQELSASI
                     LLFSSESMTLAVAVYNLYETGYLEPVAALAIVTMVIIVAAVGLATRLARGGSAMERPT
                     VAS"
     gene            6063..6689
                     /locus_tag="VLF19_03205"
     CDS             6063..6689
                     /locus_tag="VLF19_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSE92287.1"
                     /translation="MGAIAAVVFDMGGVVLGSPLAAIARYERDAGLAPNAVNRAVVAA
                     GEAGAWARLERGELTAESFCAPFEADCRASGLAVDARRLMAMMTETTVPRPRMLEAIR
                     RLRARGLKAAALTNNWVREGERVSARLRDHFDAFVESSVVGLRKPDPRIYELVCGALG
                     VAPPQAVFLDDIGRNLKPARALGMTTIKVDDPDQALRELGALLDLDLL"
     gene            6686..8311
                     /locus_tag="VLF19_03210"
     CDS             6686..8311
                     /locus_tag="VLF19_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HSE92288.1"
                     /translation="MTAEPATERDVTGAPLSFFRKWSITVTVMLITVMQILDTSVANV
                     ALPHIQGALSAGVEETAWILTSYIAANAVIIPATGWLTALLGRKRFFLICTVLFTGSS
                     FLSGLAPNLELLVLARVVQGLGGGPVVPMAQALMWEIFPLRQRGTAMAVWGIGVMMGP
                     ILGPTLGGWVADNWSWRWIFYINLPIGVLGFLMGTVFLFDNPHVRRPGRVDIPGIALM
                     IVGFGCLQLMLDWGEREDWFDSELILGLGVAAIAALTAFVIRELTAAEPVLDLTVFAS
                     RNFALGSVVIACSVFAFLSSMLLLALFTQKLLGYDAWTSGLVLAPGGFGNMLSLLIAG
                     RLVAHVDQRMLLAVGASINIVSLIWMANLSLDMGYWDMAWPRFFQGFGMGFIFVPLQT
                     LALSTVRLDRLSNATAAFNVVRNIGGSIGIALVTTLLARRAQYHQAILVEGVDMQNLA
                     MTSRIGELTAHFARQGSDGTTAARQALGTVYREVVAQAQLKAFIDDFWLLAMLFVGVM
                     LMLPLMRRVTIEQTERARAVPAGRVEGLPAASE"
     gene            8345..9688
                     /locus_tag="VLF19_03215"
     CDS             8345..9688
                     /locus_tag="VLF19_03215"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSE92289.1"
                     /translation="MRPSFRDVIARLDREGVLLRLGKEVDARHLSALTVKADRPVLFD
                     RVRGFEVRVAGGLFWSRRRLAAALGWPEAELGTRFAAGVRTMIEPETGGPAPAQEVVR
                     TGRDVDLTALPIPLLAEKDGGPYISAGVVMARSGERGLNAGVYRLMFRTRDELGIDLV
                     TVSDMRRLYERHLQRQAPLPLSVSLGVHPIEILSAAYKAPPGVSEMAIAGGLHGSPVP
                     LVPCWSVDVPAIADAEIVLEGELLPIGWTEDEGPFGEFAGMYGDLKHNPVFKVTAITH
                     RRGPIFQLLQMPWENAWLGAAATEAQVWNVLKTAGVDVVNVCVTEGSACRWSVIASIR
                     KRAGGGKNALLAILSLPDTKHALVVDEDVDIFDATQVEWARTFRVQADKDVFIIAGAQ
                     AKHVDPSVRPWELSPGQLPMTAKMGIDATIPEGIPATYYERIRHTWVDDVRLEDY"
     gene            9803..10084
                     /locus_tag="VLF19_03220"
     CDS             9803..10084
                     /locus_tag="VLF19_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92290.1"
                     /translation="MAPKLSGRFGTLMLRRGAPRRDAWVKGLPVISDAAPVAERMARL
                     LAERPRTFYELLRELPDVEYRTVLIAWGSLRERRALGRDAHGRYLIRAS"
     gene            10272..10709
                     /locus_tag="VLF19_03225"
     CDS             10272..10709
                     /locus_tag="VLF19_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HSE92291.1"
                     /translation="MLAVIVVTGCATVEESPKTTIGALGGGAFGGLIAAAAGGGGAAI
                     AGAVIGGALLGSLAGNMLDQRDKRLAAEAQHRALESGATGQPVAWTNPDTGHQGSVTP
                     VRTYQSAGTYCREFQSNVTIDGKSEKAYGTACRQPDGSWKVQG"
     gene            10722..11123
                     /locus_tag="VLF19_03230"
     CDS             10722..11123
                     /locus_tag="VLF19_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HSE92292.1"
                     /translation="MRTNLRWLTAAAALGLFLAGPPATAQTLPAPDARTWVKQQDRNG
                     DGKIDREEFHQAVVQAFFFRDKDKSGYLAIAELKEASPEALKAVKRKHDARISLQEYV
                     NALFKDFEAADADGDGLLSAEEIETYRQRAR"
     gene            complement(11163..>11533)
                     /locus_tag="VLF19_03235"
     CDS             complement(11163..>11533)
                     /locus_tag="VLF19_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92293.1"
                     /translation="ANESLAARFPDRDARQARLRAMSEAWARDFDPNSMITLRKASLT
                     FDAEPDFPKIRARVLYVLSRTDRLFPPSIAPGVMDKLARAGVDARYVEIDSDYGHLAS
                     GADWTRWAPALKTFLEALER"
BASE COUNT         1692 a         3973 c         4148 g         1720 t
ORIGIN      
        1 ggtcgcgtgg ggcgtcacgc ggggcagccg gcgggagcgg ccgaggcgcg ggagggagcc
       61 acgcgcggca tgctagcccg gccgccctcc cgcttcaagc gtctcagatg tataccgcct
      121 tgagctgggc ctcgggatag cgcgtgcccg ctgccgcgcc cgccggcacg gcctcgctga
      181 tccgccgcac gtcctcgggc gtcagcttga ccttcatgga gttgacgttc tcttcgagcc
      241 ggtccttgcg cttggtgccg gggatggcca cgacgtcgtc gccctgggcc agcagccacg
      301 cgagcacgag ctgggccggc gtgcagcgct tctcggcggc gatggcctcg atgcgcttga
      361 ccagctctac gttcttcgcg agattctcct ccttgaagcg ggggtgctcg cggcgacgat
      421 cgccctccgg gatgtcggcg accttgtgca cctggcccgt gagcaggctg cgtcccagcg
      481 gcgagtacgc gatgaacgag atgccgagcg cgcgcgtcgt ctcgcgcgtc tcctcggctt
      541 ccgtgcgata gagcagcgag tactccgtct gcacggcgct gatggggtgc acggcgtggg
      601 cccggcggat cgtctcgggc cgcgcctcgc agaggccgag gaagcgcacc ttgccctgct
      661 cgaccaggcg cttcatgccg cccacggtct cctcgatggc cacgccggga tcgacccggt
      721 gctggtaata gaggtcgatg acgtccatgc ccaggcgctt gaggctcgcg tcgcacgcct
      781 ggatgacgta ctccggccgg ccgttgacga ggttgccgcc gccgctgggg ttcttgacct
      841 gcccgaactt ggtggtgacg acggcgcggt cgcgccggcc gcgcagggcg cggcccagga
      901 gctcctcgtt gtgcccccag ccgtacatgt ccgacgagtc gaaaaagttg acgccgaggt
      961 cgagggcgcg ctggatcacg ccgatcgact gctcgtcgtc gcccttgccg tagacgccgg
     1021 acatcgtcat gaggccgagc ccgatggcgg agacgcgaag cgtgctgtgg ccgagcgtgc
     1081 gctgctgcat gcggtcctcc tcgtcgctac tggtagatct cgttgaagcg gttcttgagg
     1141 ttcttcgctt ccttctcgac gtggtcgtag tccaccggca tcagcttgat cgaggccagc
     1201 ggcgggttgc ccggcggcgg cggcacgtcg ctgcgcgcgg cgtagatgcc ctcggtgggg
     1261 aagagcttct gcacggtgtc cccgaccatg aactcggcga gcgctctggc ggcgttgggg
     1321 tgcggcgacc ccttgatgac catcgtgggc gcggcgatgg cgaaggcgcc gtcggcgggg
     1381 aagagcgtct gcaccttgtg gccggccttg cgcgcgtccc acgtgtagga gtcgaggccc
     1441 tcggcggcga cggtccgctc gccgcgggtg agcgtgtcgg agacctgctg gtggctctgc
     1501 acgatcatga tgtcgttggc gcggagcttc ttgtagaagt cccagccgta cttctgcgag
     1561 agcgtgccca ccaccatgag ctggatggcc gtgaacgacg ggtccggcat gaccagcttg
     1621 cccttccact tcgggtcggt gaggtccgtc cacgtcctcg gcggctccgg cacctggtcg
     1681 gtccggacga cgatgccgac caggttgagc cgctgggcga tgtggtaacc cctgggatcc
     1741 ttgaccgcct ccgggacctt gtcgaagttc ttcggccgga agggcaggag caggtcgcgc
     1801 ttgatcagcg cggaggccgc ggccgggtcc gagatggtca gcacgtcggc gttcatgcgg
     1861 cgggcgtccg tctcctgcat gaaccggcgc agcacggccg agccgcccga gcggaacagc
     1921 tcgaccttga taccggtctc ctgctggaag agggtcgcga tcttctgcgc ggtggccacc
     1981 ggcgtcgacg tgtaccacgt cacgctgcct tccttcctgg ccgcggggag gtcgacgggg
     2041 tccggcgtga accctccgcc ggccgaggcc gcggtgacga gcgagaacgt ggcgacgacc
     2101 gcggcgagcg cgagccggcg gatcatgccc gcctcccgtc gtgggcgtcc cggtagaccg
     2161 ggtcgggctg gctgaagaag ccgtggagga tgtgacgcac gatacggtag ttcttctgct
     2221 gcaggctcgc gtgcgcgatg cccggcatca ccgcgaactg cttgtcgggg ttcggcaggc
     2281 gctcgaagaa ctccaccagg tcctcgaacg acgcgatccc gtcgtactgc ccgcgcatga
     2341 ggatggtcgg cacggtgatc ttcgcgggct ccacgaccgg caggttcgcg cacatgtcca
     2401 cgtaggtgcc ggtggggacg gagtcgtcga gggcgaggat ggcgtcggcg aaggcctcga
     2461 tcacctcgtc gtcggcggtg ccggggtggt cgcgctcgaa gatgctgcgc acgaacgcgc
     2521 ggtcgatcgg ccgccggtgc ctggtctggt attccgccag ccgcttgcgg cgctgctcga
     2581 gggtggggct gcccttgccc gtccacacga aggcgtcgag ggccagccgg cgcacccgct
     2641 cgggccgccg ctgggcgaac agcgccgccc gaagcgcgcc cgaggagatg ccgtagacga
     2701 ggagctcgcg cacgccgcgc gtgcgagtaa tgtactcggt cgccgactcc agatcgtcgg
     2761 cgcctgtcgc gatgtcgctg ttgatggcgc gcttcttgtc ggagcggccg tagccctcca
     2821 tgtccacgca ccacgtgtcg tagccgagcc gcacgaagta gtccatggtc gaggagtcgg
     2881 cacggccggg cacgtgcaga tcgaacgtcg gcgtcgaggc catggacgag ccgtggacga
     2941 agaggatcgt cccgagccgg ccgggagagg cgccggcgcg cttctcccag agaaagagac
     3001 ggacgtcgcc cttcgtggtc cagtgctcct gtccggcgat gctcggctcc gcggtcatgc
     3061 gccgctcctt tccgtcaccc gtgacgctcg ctcacccgag gaagaccgtg cacgcgtcgc
     3121 cgtccacgcg cacgtgcacg cactggccga ccgcgagcgc ctcgccgggg ccggcctgga
     3181 cgcgcacgcg ggtgccgtcg cgcagcgtga cgtggcagtc cgtgaggttc ccgaggtacg
     3241 tcgcctcggc caccttgccg ggaatcccct cggacgtcaa cgtcaggctc tccgggcgga
     3301 cggcgacctg caccgcctgc ccgtcgccgg cctgcggggg caagggcacg cggacggtgt
     3361 gctcgccggc gagggtcacg aggccgccgt cgccgccggc gcggaccacc cggcccggca
     3421 cgatgttcac caggcccatg aagtcggcca cggcgcggct cgccggccgc gtgtagacgt
     3481 cgcgcggcga cccgtactgc agcacacggc cctcggccat gaccgcgatc cggtccgaca
     3541 gcgccatggc ctcggcctgg tcgtgcgtga cgtagacgaa cgtgatgccc gtccgccgct
     3601 gcagctcctt cagctcccac cgcatgcgct cgcgcagctt ggcgtcgagg ttggagagcg
     3661 gctcgtcgag cagcaggatc gacggctcga cgacgaggct gcgggccagc gccacgcgct
     3721 gctgctggcc gccggagagc tggttgggat agcgcgcctc gagcccggcg aggttcacga
     3781 gctccagcac gcgcgccacg cgctgtcggg cctcggcgcg ggcgaccttg cgcacctcga
     3841 ggccgaaggc cacgttctcg tacacggtct tgtgcggcca gacggcgtag ttctggaaga
     3901 ccatgcccat gccgcgctgc tcgggcggga cgacgcgctc gggcgtggag agggtgacgc
     3961 cgcccacgcg gatggcgccg gcgtccggcg cgacgaagcc ggcgacgagc cggagcagcg
     4021 tcgtcttgcc gcagccgctc ggcccgagca gcgtcacgaa ctcgcggtcg gcgacctcga
     4081 gcgtgatctc gcgcaccgcg gccacggcgc cgaaccgctt gctgaggccg tccaggacga
     4141 tgccggccat caggacgcca ccgtcgggcg ctccatggcc gagccgccgc gggccaggcg
     4201 cgtggccagg ccgaccgcag ccacgatgat gaccatggta acgatcgcca gcgcggccac
     4261 gggctcgaga taaccggtct cgtacaggtt gtagacggcc accgcgagcg tcatcgactc
     4321 cgagctgaag agcaggatcg acgcgctcag ctcctggatc gcgggcacga agacgaggag
     4381 ccagccggcg aagaggcccg gccgggccag cggcagcgag acggaccgga acgtgcgcag
     4441 ccagccggcg ccggtgatgc gcgccgtctc ctccagcgtg ggatcgatct ggcggaacga
     4501 cgcggaggcc gagcgcacgg ccagcggcac gtaccggccg gtgtaggcca ggaggatgat
     4561 cagcagcgtc ccgtagatgg gcaccggcat gcgcagccag aactggatga gggcgacggc
     4621 gacgacgatg cccggcaggc ccagcggcac cagcgaggcg tagtccatca ccttgcggcc
     4681 gggcaggcgc gtgcgcaggt cgagccaggc cacgacgctg ccgagcagca cggccagcgt
     4741 cgccgcggcg ctcgccagca gcaggctgtt ggcgatcgcc cgccgcgtca cgtcgtactc
     4801 gaagagcacg aagcggtagt tcttcagcgt caggttctgc cagaacgcga gcccccacga
     4861 tttgctgagc gacaccgcga gcagcgtgag gtagggcgcc agcacggcca cgacgaagac
     4921 ggcgacgcag aagcccagga ccgcccagcg cgccggcccg aggtccacga gctgcggccg
     4981 gctgcccttg ccgccgaggg tcacgtacga gcgccgggcc aggtaccggc gctgcagcgc
     5041 gagggcggcg accgtgatgg ccacgaagat gagcgacatc gccgaggcca ggccgtactg
     5101 cggcggatag tcgaagagcg cgtagatgcg cgtgggcagc gtgaacacgc ggccgggcag
     5161 gccgacgatg gcctgcgagc cgaagagggc gatggcgttg acgaacgcga tgagggcccc
     5221 cgacagcacg gccggggcca ccagcggcgc cgtgatcacc cccaccgtgc gccagcggct
     5281 ggcgcccagc atctgcgccg actcctccat ggaggcgtcc accgactcca gcgcgctggc
     5341 ggccagcagg tacacgaacg ggaacgtgtg cggcacggtc acgagcacga gaccggccat
     5401 ggagaagatg ttgaacgtga ccgcgggcgc accgagcacg tcgcgcagga agcggttgac
     5461 gatgccggcg ttgggcccga agaggttcac gaaggcgatg gcggtgaggt agggcggcgt
     5521 gatgtaggcc acgacggcgg tgaggtgcac gaagcgcttg cccggcaggt tcgtgcggct
     5581 caccgcccac gccatgggca ggccgacggc cacgctgatc gccgccgtcc acgtgccgag
     5641 cacgagcgag ttgatcaggg ccgtcaggta caggcggttg gcgaacgcct cgcggaagtg
     5701 gccgagcgtg agcgtgccgt cctcgctcac gctgccccac acgagcgagc cgagcggcag
     5761 gatcaccagc acggccaggg cggccaccgc gcaggccatg acgacgtgct ccgcccggag
     5821 gacggaggcc ggggccgcgg tacgggtgac gacgctcacg gacgtccggc ctggctacgc
     5881 tggctcatcg ggagaatcgc cggagtctag cacggggcgt cgacggcctg gagtccggag
     5941 cgcgaaggtc gctcatcagg attccgagga ccggtcctgc tgaccgagcc tggctggatc
     6001 ttatgccaaa cgcgacacga cgacgcgtcc ggcgtcgccg cgatgcgtcg tacaatcgcc
     6061 gcgtgggcgc gatcgcggcg gtcgtcttcg acatgggcgg cgtggtgctc ggctcgccgc
     6121 tcgcggcgat cgcgcgctac gagcgcgacg ccgggctcgc gcccaacgcc gtcaaccggg
     6181 ccgtcgtggc ggccggcgag gccggggcgt gggcgcggct ggagcgcggc gagctgacgg
     6241 cggagtcgtt ctgcgcgccg ttcgaggccg actgccgcgc cagcggcctc gccgtggacg
     6301 cccgccggct catggccatg atgaccgaga ccacggtgcc ccggccgcgg atgctggagg
     6361 cgatccggcg cctccgcgcg cggggcctca aggcagccgc gctcacgaac aactgggtgc
     6421 gcgagggtga gcgggtgtcc gcgcgcctgc gcgaccactt cgacgcgttc gtggagtcct
     6481 cggtggtggg cctgcgcaag cccgacccac gcatctacga gctggtgtgc ggcgcgctcg
     6541 gcgtggcgcc gccgcaggcc gtgttcctcg acgacatcgg gcgcaacctc aagccggcgc
     6601 gcgcgctcgg catgacgacg atcaaggtgg acgaccccga ccaggcgctc cgcgagctcg
     6661 gcgctcttct ggacctagac ctcctgtgac cgcggagccc gccaccgagc gcgacgtcac
     6721 cggcgcgccg ctgtcgttct tccgcaagtg gtccatcacg gtgacggtga tgctgatcac
     6781 ggtcatgcag atcctcgaca cgagcgtcgc caacgtcgcg ctgccccaca tccagggcgc
     6841 gctgtcggcc ggcgtcgagg agactgcctg gatcctcacc tcctacatcg ccgccaacgc
     6901 cgtcatcatc cccgccaccg gctggctgac cgcgctcctg gggcgcaagc ggttcttcct
     6961 gatctgcacg gtgctcttca ccggcagctc cttcctgtcc ggcctggccc cgaatctgga
     7021 gctcctcgtg ctcgcgcgcg tcgtccaggg gctcgggggc gggccggtcg tcccgatggc
     7081 gcaggcgctc atgtgggaga tcttcccgct ccgccagcgc ggcaccgcga tggccgtgtg
     7141 gggcatcggc gtcatgatgg gcccgatcct cggcccgacg ctcggcggct gggtcgccga
     7201 caactggtcg tggcgctgga tcttctacat caacctgccg atcggcgtgc tcggcttcct
     7261 catgggcacg gtcttcctct tcgacaaccc gcacgtccgt cgtcccggcc gcgtggacat
     7321 tcccggcatc gcgctcatga tcgtcggctt cggctgcctg cagctcatgc tcgactgggg
     7381 cgagcgcgag gactggttcg actcggagct gatcctcggg ctcggcgtgg ccgccatcgc
     7441 ggcgctcacc gccttcgtga tccgggagct gaccgcggcg gagccggtcc tcgacctcac
     7501 ggtcttcgcc tcgcgcaact tcgcgctcgg ctccgtcgtc atcgcgtgct ccgtcttcgc
     7561 gttcctctcc agcatgctcc tgctcgcgct cttcacccag aagctcctcg gctacgacgc
     7621 ctggacctcg gggctcgtgc tcgccccggg cggcttcggc aacatgctct cgctgctcat
     7681 cgccggccgc ctcgtcgctc acgtggacca gcggatgctg ctcgcggtcg gcgcctccat
     7741 caacatcgtc tcgctcatct ggatggcgaa cctcagcctg gacatgggct actgggacat
     7801 ggcctggccg cgcttcttcc agggcttcgg catgggcttc atcttcgtgc cgctgcagac
     7861 gctcgcgctc tcgacggtgc gcctcgaccg cctgagcaac gccaccgcgg cgttcaacgt
     7921 ggtccgcaac atcggcgggt ccatcggcat cgcgctggtc accaccctgc tcgcccgccg
     7981 cgcccagtac caccaggcca tcctggtcga gggcgtggac atgcagaacc tggcgatgac
     8041 atcacggatc ggcgagctga cggcgcactt cgcccgtcag ggctccgacg gaacgaccgc
     8101 cgcccgccag gcgctcggga cggtctatcg cgaggtggtg gcccaggcgc agctgaaggc
     8161 cttcatcgac gacttctggc tgctggcgat gctgttcgtc ggcgtcatgc tcatgttgcc
     8221 gctgatgcgc cgggtgacca tcgagcagac cgagcgcgcg cgggccgtgc ccgccggccg
     8281 cgtggaaggc ctgcccgcgg ccagcgagtg aggtctgcta tcctccgtgg ccggagagga
     8341 cacgatgcgc ccatcgttcc gtgacgtgat cgcacggctc gatcgtgagg gcgtgctgct
     8401 gcgcctgggc aaggaggtcg atgcgcgtca cctctccgcg ctcacggtga aggccgaccg
     8461 gcccgtgctc ttcgaccgcg tccgcggctt cgaggtcagg gtcgcgggcg gactcttctg
     8521 gagccgccgc cggcttgccg ccgcactggg ctggccggag gccgagctgg gcacacgctt
     8581 cgccgccggc gtgcggacca tgatcgagcc ggaaaccggg gggccggcgc cggcgcagga
     8641 ggtcgtccgc acggggcgcg acgtggacct caccgcgctg ccgatcccgc tgctggccga
     8701 gaaggacggg gggccgtaca tcagcgctgg cgtggtcatg gcccggagcg gcgagcgcgg
     8761 gctcaacgcc ggcgtctacc gactcatgtt ccgcacgcgc gacgagctgg gcatcgatct
     8821 cgtcaccgtg tccgatatgc gccgtctcta cgagcgccac ctccagcggc aggcgccgct
     8881 gccgctctcg gtgtcgctgg gcgtccaccc gatcgagatc ctgagcgcgg cctacaaggc
     8941 accgcccggc gtgagcgaga tggccatcgc cggcgggctg cacggatcgc ccgtgccgct
     9001 cgtgccgtgc tggagcgtcg acgtgccggc catcgccgac gccgagatcg tgctcgaggg
     9061 cgagctgcta ccgatcggct ggacggagga cgagggtccc ttcggcgagt tcgccggcat
     9121 gtacggcgac ctcaagcaca acccggtgtt caaggtgacg gcgatcaccc atcgccgcgg
     9181 cccgatcttc cagctcctgc agatgccgtg ggaaaatgcc tggctcggcg ccgcggccac
     9241 cgaggcgcag gtgtggaacg tgctcaagac ggccggcgtc gacgtcgtca acgtctgcgt
     9301 caccgagggc agcgcgtgcc ggtggtcggt catcgcctcg atccgcaagc gcgccggcgg
     9361 cggcaagaac gcgctgctgg cgatcctctc gctgcccgat accaagcacg ccctcgtcgt
     9421 ggacgaggac gtggacatct tcgacgcgac gcaggtcgag tgggcgcgga ccttccgtgt
     9481 gcaggccgac aaggacgtgt tcatcatcgc cggcgctcag gccaagcacg tggacccgag
     9541 cgtccgaccc tgggagctgt ccccgggcca gcttccgatg accgcgaaga tgggcatcga
     9601 cgccacgatc cccgagggca tcccggccac gtactacgag cgcatcaggc acacgtgggt
     9661 ggacgacgtc cggctcgagg attattgaca tgggggcccc gacatggccc ccaagccccc
     9721 cgggcggctc ggcacgcccc ggccaagccg tggcgcgcct cgaagacccg ccgaggggat
     9781 tcggtgacat gggagccccg acatggcccc caagctctcc gggcggttcg gtacgctcat
     9841 gttgcgccgt ggcgcgcctc ggcgcgacgc atgggtcaag ggactaccag tgatcagcga
     9901 cgcggcgccg gtggccgagc gcatggcgcg cctgctcgcc gagcggccgc ggacgttcta
     9961 cgagctgctc cgcgagctgc ccgatgtcga gtaccgcacg gtcctgatcg cgtggggctc
    10021 gctgcgcgag cgccgcgccc tcggccgcga cgctcacggc cgctatctga ttcgcgcgag
    10081 ctagggcgga gcttgtgccc gaatgctcac ttcctcgtgg cgggccatgg cccagatgtc
    10141 ataccgatcg agccagacca cgggctacca gttgcgcctg atcagcaacc gggggagacc
    10201 ctgacatgac ttatcatctc gcggggaggg cgctcatgcc gggatcaatc atgcggtgcg
    10261 gagcggtctt gatgctcgct gtcatcgtgg tgacgggatg cgccaccgtc gaggagagcc
    10321 cgaagacgac aatcggcgcc ctgggcggtg gagcattcgg cgggttgatc gcggcggccg
    10381 cgggcggcgg gggtgcggcc atcgcgggcg ccgtgatcgg cggggctctc ctgggcagcc
    10441 tggcgggtaa catgctcgac cagcgcgaca agcggctggc ggcggaggcg caacaccggg
    10501 cacttgagtc gggggccacc ggtcagcccg tcgcgtggac gaaccccgac accgggcacc
    10561 agggctcggt gacgcctgtc cggacgtatc agtccgccgg cacctactgt cgcgagttcc
    10621 agagcaacgt gaccatcgac ggcaagagcg agaaggccta cggcaccgcc tgccgccagc
    10681 ccgacggcag ctggaaggtt cagggctgag aggagaggat gatgcgcacc aacctccgct
    10741 ggctcaccgc ggcagccgcg ctgggcctgt tcctggccgg cccgccggcg acagcacaga
    10801 cgctccccgc cccggatgcg aggacgtggg tgaagcagca ggaccggaac ggcgatggca
    10861 agatcgatcg cgaggaattc caccaggcgg tggtccaggc cttcttcttc cgcgacaagg
    10921 acaagagcgg ctacctcgcg atcgcggagc tcaaggaagc cagtcccgaa gccctcaagg
    10981 ccgtcaagcg gaagcacgat gcccggatct ccctgcagga gtacgtcaac gcgctcttca
    11041 aggacttcga ggccgccgat gccgacgggg atggcctcct gtcggcggag gagatcgaga
    11101 cgtatcggca gagggccagg tagagaacag aggaatcgag tgaagtcgat tcactggatc
    11161 ttctagcgct ccagcgcctc caagaacgtc ttcaacgctg gcgcccacct ggtccagtcg
    11221 gcgccgctgg cgaggtggcc gtagtcactg tcgatctcga cgtagcgcgc gtccacgccc
    11281 gctctggcga gcttgtccat cacgccgggc gcgatggagg gcgggaacag ccgatcggtg
    11341 cgcgagagca cgtagagcac cctggcgcgg atcttcggga aatccggctc ggcgtcgaac
    11401 gtcagcgagg ccttgcggag cgtgatcatc gagttcgggt cgaagtcgcg ggcccaggct
    11461 tcggacatcg cacgcaggcg agcctggcgc gcgtcgcggt ccgggaagcg ggcggccagc
    11521 gactcgttgg cgc
//
LOCUS       DATIAZ010000094         6261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_943107, whole genome shotgun sequence.
ACCESSION   DATIAZ010000094 DATIAZ010000000
VERSION     DATIAZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943107"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..2628)
                     /locus_tag="VLF19_03240"
     CDS             complement(121..2628)
                     /locus_tag="VLF19_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92294.1"
                     /translation="MRHRRASGLGWGAAPLALLGLSVHAGGVALLSIVGPLPGLPVPD
                     WMPLAVPLAVYAVVAVLAVHRFAIAPIAGTFATACLLHALLVVLPGILAPSVAALAGV
                     RSVTPPASTSTALALLTALSVTLTLTPFRRLVAARSRYARTAARVGYPGVRPVRDLAE
                     LAAPRPPGVTLPPVVTAPAAPAGPPPAPSVPEPAKAVPPGLPVVPPLVVPRPTPPVPR
                     APSRPTVPSPAPVPPPPPRPAEVPAPSLASSAPAGRPPGAEPMEEMVRIAFARVADQL
                     PPELFKLAPDRLGANLLEAGFLLVPRRLVVPQLAEGFVRVPWEIVADQFPRQALAVPE
                     RDVKTRIANGAIVLPLDEVIRQVPPDFFTLTSPELDVRGLENFPPPFQPHVPPPSASR
                     AEAPGLEASELEAAELEASEPEAPEREAPGLEAPPDDSAGNLLEEDPAPGDPPGIAAF
                     SSDERQAPPVPLGASEPLAPAWSEPPLAGDPEAFDDPLREAEIAELERAMAAPDAESA
                     APFDDELPSALESVESGAPLPPAASVELAASSEPDEPFSLAPPVEGLSPAGPTALPHP
                     DVPAIVTATARLRIRGIAALLAPLITPLETAAVAHGGRTVLTVLPPAMDETAAIDIAL
                     RVVPYLIDPRLPSPAAQATVRTRTSAFVVTPLTPGDAGGDVLLTGTPAGASLALLERL
                     SLQAARDWHAVHGVPRPPRPRRDVPGDEALRPTSVPPHVRSLAESLRAFGAVSPAVLR
                     DVEGSLLVYLFLPQGLEPRALGALAWDLTRALAGVPLGPVESVVLRAGTEGRLVVRVL
                     HEAAGHATVLVAGGGPVDRPGLARIELDRAAARLGAL"
     gene            complement(2628..3491)
                     /locus_tag="VLF19_03245"
     CDS             complement(2628..3491)
                     /locus_tag="VLF19_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92295.1"
                     /translation="MAKVLVVDDSVSVRKVVAKALEGRSMEVLSAASGREAIESIERD
                     RPDLVVCDVILPDKDGYQVCEFVRAHPAVGRTPVLLISGVVNSTVLARAAEVQSTDVM
                     FKPFAAEELVRKIDTLLGSRTGAANGRAAVTRMPVASHAAAPLAGAPPASAPPVTPPR
                     GTVAPGGVGPGAGLKGRLNALAAAAGVHFVALADREGFLIESAGDLPPQVEVAGAMAA
                     CLTESSDGVGRELGQGALIGMILEYESGVVLLNGVGANALLAVCVGDVSALGKVRYFV
                     KKALPDLERAL"
     gene            complement(3511..4347)
                     /locus_tag="VLF19_03250"
     CDS             complement(3511..4347)
                     /locus_tag="VLF19_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HSE92296.1"
                     /translation="MSLPPRILVIDADRPTLAALEKALVGAGFTNVTGVTSGSFALTM
                     LERDRPTLIVSRARVPDVGGWELCSIVRSDPSMAGVLFLLLAASGDDVPEDALDGGPD
                     RMLVGEFTPETIVAEVAGLLSAATAVPRAAAGERAPGGLRGSLAVIDLPDLAQAIALG
                     GKTGDLRLTLGAAAGRIVFDRGRVVHAEFGPLTGEAAFAALVATAQRGDHGTFAFEPC
                     ERVDPGVPRTIDRSVKQLLLGIAAAIDERHAGPAIARTNGKTAGGAVTGSIEPRARPP
                     RD"
     gene            complement(4344..>6261)
                     /locus_tag="VLF19_03255"
     CDS             complement(4344..>6261)
                     /locus_tag="VLF19_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92297.1"
                     /translation="VTPFAIPRAAVGVGAASTPPVVRPSIRVNLDRLDALMNLAGELV
                     IARARLDRHLTQLGHVGDLLGFTQGRMRRAVGEFETKYANPHLPAAAAGPGGHDGPPA
                     EPAAVTLDGVFAELEFDRYDDFNIFARRAGEIVSDMGEVQTQLASLVRAVRADAARVQ
                     QLGAALRVEITRSRMVPLGRLFGRFARQVRETARTAGKQVTLEIVGDAVEVDSGIIDQ
                     ITDPLLHLIQNAIVHGIEGPEERVKRGKPPQGSLRLRAVHKGGSFHVEVADDGRGIDT
                     TAVRAAVERAGLASHDALAYLDDRTVLDLIFVPGVSTAEEVTTIAGRGVGMDVVRTNV
                     GRLGGEIEVDTTPGAGTRFTLKLPLTMAISDALFVRVGDQTLAVPVHSIKGMARVRPD
                     EVIVADGAETVVVEDERLELLHLDRVLGLAGAPADGPTPVVVLRTGRRALAVAVDELL
                     AREEIVVKRLGAFLDGAGPYLGATVSPEGRVILLLDAGRLAESAGPVAWPRRAAVAAS
                     QLRSRTVLLVDDSVSVRKFVGQMLERAGFRVVTANDGVEALDRLAEISVDVVVTDLEM
                     PRVNGYELVRDLRRRAATRQVPIVVLTTRTGDKHMRLARQLGVEHYVSKPVDEAAFVR
                     LVDTLATEAAGAVA"
BASE COUNT          902 a         2325 c         2290 g          744 t
ORIGIN      
        1 ggcaggcacc ggtggatgta ctgcagattc gccgtcttgc cggccactcc ggtgccgtag
       61 tacacgagct tgaagtggac gaccttctcg gcgtggttga tgatggccac ggtcacgggc
      121 tcacagtgcg ccgagccgcg ccgccgcgcg gtccagctcg atgcgggcca ggccaggacg
      181 gtccacgggg cctccgcccg cgaccaggac cgtcgcgtgt cccgccgctt cgtgcagtac
      241 ccgcaccacc agacggccct cggtgcccgc gcgcaagacg acggactcca ccgggcccag
      301 cggcaccccc gcgagcgccc gcgtgaggtc ccacgcgagc gcaccgagcg cgcgcggctc
      361 gagcccctgc ggcaggaaca agtagaccag cagcgatccc tcgacgtcgc ggagcacggc
      421 tggcgagacg gcgccgaacg ctcgcaacga ctcggccaag ctccggacgt gcggcgggac
      481 gctggtcggc ctcagggcct cgtcgccggg gacgtcgcgc cgcggacgcg ggggccgcgg
      541 gacaccgtgc accgcgtgcc agtcccgcgc cgcctgcagc gacagacgct cgagcagcgc
      601 caacgatgcc cccgctggcg tcccggtcag caagacgtct ccgccggcgt cgccgggagt
      661 cagcggcgtc acgacgaacg cgctcgttcg cgtgcgaacc gtcgcctggg ccgcgggcga
      721 gggcagacgc ggatcgatca ggtacgggac caccctgagc gcgatgtcga tggccgccgt
      781 ctcgtccatc gccggcggca gcaccgtcag gaccgtccgg ccgccgtgag ctacggcggc
      841 cgtctcgagc ggagtgatca gcggcgcgag cagcgcggca atcccgcgga tccgcagacg
      901 ggccgtagcc gtcacgatcg ccggcacgtc ggggtgcggc agcgccgtgg gaccggccgg
      961 ggagagcccc tcgaccggcg gcgccaggga gaacggctcg tcgggctccg acgacgctgc
     1021 gagctcgact gacgccgcgg ggggaagcgg cgcgcctgac tcgaccgatt caagcgccga
     1081 gggcagctca tcgtcgaacg gcgcggccga ttcggcgtcc ggcgccgcca tcgcgcgttc
     1141 gagctcggcg atctccgcct cccgcagcgg gtcatcgaac gcctcggggt cgccggcgag
     1201 gggcggctcg gaccacgcgg gcgccagcgg ctcgctggcc cccagcggca ccggcggggc
     1261 ctggcgctcg tcgctcgaga acgcggcgat ccccggcgga tcacccggcg ccggatcctc
     1321 ctccaggagg ttgcccgcgg agtcgtcggg gggggcctcg agcccgggag cctcgcgctc
     1381 gggagcctcg ggctcggaag cttcaagctc ggcagcttcg agttcggacg cctcgagccc
     1441 gggcgcctcg gctcgcgacg ccgagggcgg cggaacgtga ggttgaaacg gtggaggaaa
     1501 gttctcgagg ccgcgcacgt ccaactcggg cgatgtgagc gtgaagaagt cgggcggtac
     1561 ctgccggatg acctcatcca gcggaagcac gatcgcgccg ttggcgatgc gagtcttgac
     1621 atccctctcg ggcaccgcga gggcctgacg cgggaactga tcggccacga tctcccaggg
     1681 cacgcgcacg aatccctcgg ccagctgcgg caccacgagg cgacgcggga cgagcaggaa
     1741 tcccgcctcg aggaggttcg caccgagccg gtcgggcgcc agcttgaaca gctcgggcgg
     1801 gagctgatcc gccacacggg cgaaggcgat gcggaccatc tcctccatcg gctccgcgcc
     1861 tgggggacgt ccggccgggg ccgaggacgc caacgacggc gccggaacct ccgctggccg
     1921 tggaggtggc ggcggcacgg gagccgggga gggcacggtc gggcgggacg gcgcccgggg
     1981 caccggcggc gtcggccgcg gcacgacgag cggcggcacg accggcaagc cgggcggcac
     2041 cgccttcgcc ggctcgggca cggacggggc agggggcgga ccggccggcg cagcgggcgc
     2101 ggtcacgact ggtggcagcg tgacccccgg cgggcgcggc gccgccagct ccgccaggtc
     2161 ccgcacaggg cggaccccgg gataccccac gcgtgccgcc gtccgcgcgt agcgcgagcg
     2221 cgctgccacc agccggcgga agggagtcag cgtcagcgtg accgagagcg ccgtgagcag
     2281 ggccagggcg gtggacgtcg aggccggcgg cgtcacgctc ctcacaccgg cgagcgccgc
     2341 aaccgaaggc gccaggatgc ccgggagcac gacgagcagg gcgtgcagca ggcacgccgt
     2401 ggcgaaggtc ccggcgatcg gagcgatcgc gaaccggtgg accgcgagca cggcgacgac
     2461 ggcgtacacg gccaacggta ccgcgagcgg catccagtcg ggcaccggca gccccggcag
     2521 cgggccgacg atcgacagca gcgcgacgcc gccggcgtgg acgctgagac ccagcagggc
     2581 caatggagcg gccccccatc ccagccccga cgcgcgccgg tggcgcatca gagcgctcgc
     2641 tccaggtcgg gtagcgcctt cttgacgaag taccggacct tgccgagcgc cgagacgtcg
     2701 ccgacacaca cggcgagcaa cgcgttcgca ccgacgccgt tcaggagcac cacgcctgac
     2761 tcgtactcga ggatcatccc gatcagtgcc ccctgtccga gctcgcgccc gacgccgtcc
     2821 gaggactccg tcaggcacgc cgccatcgct cccgcaacct ccacctgggg cggcaggtcg
     2881 cccgcggact cgatcaggaa gccctcgcgg tccgccaggg cgacgaagtg cacgcctgcc
     2941 gccgccgcaa gcgcgttcag ccgtcccttg agaccggcgc ccggcccgac gccgcccggc
     3001 gccacagttc cccgcggcgg ggtgacgggc ggcgcactgg ccggcggcgc gccggccagc
     3061 ggagccgcag cgtgtgacgc cacgggcatc cgtgtgacgg ccgcgcgccc gttggcggcg
     3121 cccgtccggc tgcccagcag cgtatcgatc ttccgcacaa gctcctcggc ggcgaacggc
     3181 ttgaacatga cgtcggtcga ctgcacctcg gcggcgcgcg cgagcacggt gctgttcacc
     3241 acgcccgaga tgaggagcac cggcgtgcgg ccgaccgcgg gatgcgcgcg gacgaactcg
     3301 cacacctgat agccgtcctt gtccggcagg atcacatcgc agacgacgag gtcggggcgg
     3361 tcccgctcga tgctctcgat cgcctcgcgg cccgaggcgg ccgagagcac ctccatgctg
     3421 cgaccctcga gcgccttcgc gaccaccttc cgcacgctca cgctgtcgtc gacgacgagc
     3481 accttcgcca tcctggcctc cgtcccagcc ctagtcgcgc ggcggtcgag cccgaggctc
     3541 gatcgatccg gtcacggcac ctccggccgt cttcccgttc gtccgggcga tggcggggcc
     3601 ggcatgcctc tcgtcgatcg ccgccgcgat gccgagcagc agctgcttca cgctgcggtc
     3661 gatggtccgc ggcacgcccg gatcgacccg ctcgcagggc tcgaacgcga aggtaccgtg
     3721 gtcccctcgc tgggccgtgg cgaccagcgc ggcgaacgcg gcctcgccgg tcagcggccc
     3781 gaactccgcg tgcaccacgc gaccgcgatc gaagacgatc cgcccggcgg cggcgccgag
     3841 cgtaagtcgc aggtcgccgg tctttccccc gagcgcgatc gcctgcgcca ggtccggcag
     3901 gtcgatcacc gccagcgagc cgcgcagccc cccgggcgcg cgctcgccgg cggcggcgcg
     3961 gggtaccgcg gtggcagccg acagaaggcc ggcgacctcg gccacgatcg tctccggcgt
     4021 gaactctccg acgagcatgc ggtcgggccc gccgtcgagg gcgtcctcgg gaacgtcgtc
     4081 gccggaggcg gcgaggagga ggaagagcac gccggccatc gacggatcgc tccggacgat
     4141 cgagcagagc tcccagccgc cgacgtcggg cacgcgggcg cggctcacga tgagcgtcgg
     4201 gcggtcacgc tccagcatcg tgagcgcgaa cgagccgctg gtcacgccgg tcacgttcgt
     4261 gaacccggcg ccgacgagcg ccttctcgag cgccgcgagc gtcggccggt cggcatcgat
     4321 gaccaggatc cgcggcggga ggctcacgcc acggcccccg ccgcctcggt cgccagcgtg
     4381 tccacgagcc gcacgaacgc ggcctcgtcc acgggcttgc tgacgtagtg ctcgacgccg
     4441 agctggcgcg cgagccgcat gtgcttgtcg cccgtccggg tcgtgagaac cacgatgggg
     4501 acctggcgcg tggccgcccg gcgccgaaga tcccgcacga gctcgtagcc gttcacgcgc
     4561 ggcatctcga ggtcggtgac gacgacgtcc acgctgatct cggcgagccg gtcgagcgcc
     4621 tcgacgccgt cgttggcggt gaccacgcgg aagcccgcac gctcgagcat ctgaccgacg
     4681 aacttgcgca cgctgaccga gtcgtcgacg agcaacaccg tgcgcgaccg cagctgactc
     4741 gcggccacgg ccgcgcgccg cggccaggca acgggaccgg cgctctcggc gaggcggccg
     4801 gcgtccagca acaagatgac gcgcccctcc ggcgacaccg tcgcccccag gtacggcccg
     4861 gcaccgtcga gaaaggcgcc gaggcgcttg acgacgattt cctctctcgc cagcagctcg
     4921 tccacggcta cggccagggc gcggcggccg gttcgcagca cgacaaccgg cgtcgggccg
     4981 tcggcgggcg cgccagcgag cccgagcacg cggtccaggt gcagcagctc cagccgctcg
     5041 tcctcgacca cgaccgtctc ggcaccgtcg gcgacgatga cctcgtccgg gcgcacccgc
     5101 gccatcccct tgatcgaatg gaccggcacc gcgagcgtct ggtcgcccac gcggacgaac
     5161 aacgcatcgg agatcgccat cgtgagcggc agcttcaggg tgaaccgggt gccagcgcca
     5221 ggtgtcgtgt ccacctcgat ctcgccgccg agacgtccca cgttggttcg caccacgtcc
     5281 atgccgacgc cgcggcccgc gatcgtggtc acctcctccg ccgtgctcac gccggggacg
     5341 aagatgaggt cgagcacggt gcggtcgtcg aggtacgcga gggcgtcgtg gctggccagc
     5401 cccgcccgtt cgactgcggc ccgtacggcc gtcgtgtcga tgccccggcc gtcgtcggcg
     5461 acctccacgt ggaagctccc gcccttgtgg accgcgcgga gccggagcga cccctgcggc
     5521 ggcttgccgc gcttcacccg ttcttccggg ccctcgatgc cgtggacgat cgcgttctgg
     5581 atcaggtgga gcagcggatc cgtgatctgg tcgatgatcc cgctgtcgac ctcgacggcg
     5641 tcgccgacga tctcgagcgt gacctgcttg ccggcggtcc gcgcggtctc gcgaacctgc
     5701 cgtgcgaagc gtccgaacag gcgccccaga ggcaccatcc gcgaccgtgt gatctcgacg
     5761 cgcagggcgg ctccgagctg ctggacgcgg gcggcgtcgg cacggacggc acggacgagg
     5821 ctggcgagct gcgtctgcac ctcgcccatg tcgctgacga tctcgcccgc gcggcgcgcg
     5881 aagatgttga agtcgtcgta gcggtcgaac tcgagctccg cgaacacgcc gtcgagggtg
     5941 accgcggcgg gctccgcggg cgggccgtcg tgacctcccg ggcccgcggc cgccgccggc
     6001 aggtgcgggt tggcgtactt ggtctcgaac tccccgacgg cccgacgcat ccgtccctgc
     6061 gtgaacccca ggaggtcgcc cacgtgcccg agctgcgtga ggtgacggtc gagccgcgcg
     6121 cgcgcgatga ccagttcgcc cgcgaggttc atgagcgcgt cgagacggtc gaggttgacg
     6181 cggatgctcg ggcggacgac agggggtgtc gaggccgcgc cgacgccgac ggccgcacgc
     6241 gggatcgcga agggcgtgac g
//
LOCUS       DATIAZ010000095         8581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_550044, whole genome shotgun sequence.
ACCESSION   DATIAZ010000095 DATIAZ010000000
VERSION     DATIAZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550044"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VLF19_03260"
     CDS             complement(<1..923)
                     /locus_tag="VLF19_03260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSE92298.1"
                     /translation="MHGVSSTGGATVAAPIILRHRRIRAMTETLLTLLKASAGALILT
                     IGMGSTPADFTYLASLFAFGPARGSAIDARGAGGEAAAWSARFAAEVDRRLDVPPNDQ
                     QRYAVMLAQALAQAGVSDRGAQAFVLIDRSPQVQAGFVVLRTPAGGWHWLGAAPVSTG
                     RPSGFEHFRTPTGVFAHTLDNPDFRAEGTLNEHNVRGYGLAGMRVFDFGWVAAERGWG
                     RGGTSLMRLQMHATDPDRLEPRLGRAESKGCIRIPATLNTFLDRHGILDADYDAALAR
                     GDVLWVLRADREPIPWPGRYLVVVDSQAATRP"
     gene            complement(1036..1740)
                     /locus_tag="VLF19_03265"
     CDS             complement(1036..1740)
                     /locus_tag="VLF19_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92299.1"
                     /translation="MLQLEGVRAAYGPVEVLRGLDLEVREGELVCLIGANGAGKTSTL
                     RTISGLLRPTHGRITLAGREIHGHSPADILTAGIAHCPEGRRVFPHMTVHENLMMGAH
                     VRRDRAEVVRDVERLSQHFPILAQRRRQAAGTLSGGEQQMLAIARALMARPRLILFDE
                     PSLGLAPTIVEAVFAIIDDIRRRGTTVLMVEQNAHLALGMADRGYVMETGRVALAGAA
                     RDLAADDHVRRAYLGG"
     gene            complement(1740..2558)
                     /locus_tag="VLF19_03270"
     CDS             complement(1740..2558)
                     /locus_tag="VLF19_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92300.1"
                     /translation="MTLAVRDLGVRFGGVEALGGVSFEVRPDTVTSLIGPNGAGKTTA
                     FNAITGYLRPTRGEVLWEGRPITRLRPSAIARRGIVRTFQKTSVFPSLAVLDNVLIGL
                     HLRGRAGWAAALAGRRWVRDEEHVLAVEAEEILAFVGLAARRREPAGALPYGEQRLVE
                     LAVALAARPRMLLLDEPAAGMTSAEKTVTSALIRRIRDQGVTVFVVEHDMRLVMGISD
                     TVIVLSGGRLIAEGPPAAIQSHPEVVRAYLGSTRVPAGGPGAAGAGDDPARAGS"
     gene            complement(2555..3490)
                     /locus_tag="VLF19_03275"
     CDS             complement(2555..3490)
                     /locus_tag="VLF19_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92301.1"
                     /translation="MRRLLPALGAIVVLTVPLWVQSPYHLHVIIMAGIFVILASSLNL
                     LLGYAGQLSLGHAAFFGIGAYTSALLTLRLEWSFWAGLACGTVAAGLAGWAIGRLALK
                     VRGAYFVLVTISFAGVISLVSVNWMDLTNGPLGLPGVPAPELFGWSLRSKSAYYYLVL
                     AGAALAALVCRRLVRSRVGRAFLALRENEALAESVGIDAVHYLVVAAVTSAALAGMAG
                     SLYAHYTRLVSPEVFLFSYTVTMVIMVVAGGKGTLSGPLVGAVLFTVLPEALREVVAW
                     QWQMVLYGVLLVALVFFLPRGIVPALAGWRARRSA"
     gene            complement(3606..4478)
                     /locus_tag="VLF19_03280"
     CDS             complement(3606..4478)
                     /locus_tag="VLF19_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92302.1"
                     /translation="MEQLLQHLVNGLVLGGTYALLGIGLTLIFGLMNVVNFAHGELYT
                     LGAYAAFTALALAEVSFFAAVAAALLVGAAAGAACERVLLRPLHGESIDTTMLVMIGV
                     SIALQNAVLLGFGGVAKSIPHPFPTEPIVAGPVSIAPLRLVVLGVAAALIAGSYLVIH
                     RTRLGQAMRATFQDPDTAQLMGVRIHRVHTATFAFGAGLASAAGALLGPVFLAYPSMG
                     DLAALRAFCVVILGGLGNLPGATLGGLILGIAEELGAGYVSSGYRDAVGFVMIIGVLL
                     LRPSGLFARAERIG"
     gene            complement(4495..5667)
                     /locus_tag="VLF19_03285"
     CDS             complement(4495..5667)
                     /locus_tag="VLF19_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92303.1"
                     /translation="MRKRWAVPLTVVAGVALLVAGVAESQAPIKIGVIQPLSGPVAAS
                     GNYVRMGAEIARDWINAKGGVGGRKVELVIEDNKSDPKEAASAAEKLIVRDKVPAIMG
                     AWGSSMTLAAMPKLEEYGVPMVVETSSAASITRRGNPWIFRISPPSEMEALGLEKYLA
                     PLGIKKADFLAVNTDWGRGAITAFGDVLKKHNVTVGVTEFMDQAATDMNAQITKVKGT
                     GGDTLFLTTAVEQITLVLKQSQEQRLGRKIITTGGSSSPTQLIKQAGPAAEGSYHIVF
                     FMPWFPEAMPDGKLAKAFVDEWAKRGHPFEGLTEGFRGHDGIATIAEAVKVAGKDDPK
                     ALREALWKVSLMGVNGPIKFAKDGPAGKESGQSRPSIFIVQIKDGKVALPNFVARK"
     gene            complement(5739..7499)
                     /locus_tag="VLF19_03290"
     CDS             complement(5739..7499)
                     /locus_tag="VLF19_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSE92304.1"
                     /translation="MARAEYGSDVVVDLMRAFGIEYVALNPGATFRGLHDSLVNYGGN
                     TRPELILCCHEEIAVALAHGYAKATGKLMAAAVHDVVGLQHACSAIFNAFCDRAPVFV
                     VGATGPMDATKRRPWIDWIHTALVQGQQVRDYVKWDDQPASIAAIPEAFTRAWRLAST
                     PPRGPVYLCLDAALQEEALTGPMAIPDPGRFAPPAPPQAEGRALETAARLLAEAASPV
                     IVVEALARAPEALAALRRLAERLAAPVVDTSQSSRGRPSFPGSHPLDATDERENALRE
                     ADVILALDVEDYLGALGTTDRATRRVRSLAAPSMKLISISLNDYAFRSWAHTFQSLVP
                     VDVPIAADTAVALPTLDALVEDRLRHDRRAPERQARLERCRRGHAARQGEWRAAVDAR
                     RTARPLALPVLAAEVWAAIAGEDWVLTNGVAGGWARRLWDWQPERAFGGSGGAGLGYG
                     LPASLGMALAFRNTSRLCVNLQSEGDLLYVASAFYTAAHHRLPLLTVICNNGTYYNDE
                     MHQEAVARERGRPVERKTVGIRIEDPAPDYGRIAAAFGIHAEGPIDDAAALGPALKRA
                     LRVVKEERRPAVLDVLTGVA"
     gene            7690..>8581
                     /locus_tag="VLF19_03295"
     CDS             7690..>8581
                     /locus_tag="VLF19_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSE92305.1"
                     /translation="MNVQTRAPAGRGVVALGLLAMCPLATSEHVAAGDPSTAGRVLRV
                     GPGRELARPSHAAALARAGDTVEIDAELYEGDVVTWRADHLTLRGVGGRARLRAAGQA
                     AEAKAIWVIRGRNTVVEDVEFSGARVPDRNGAGIRQEGADLTIRRCLFHDNENGVLLG
                     GGPDSETVIEESEFAANGAGDGRSHNIYIVAVRRFVLRGSDVHHARTGHNVKSRAREN
                     VIVGNRIVDEATGTSSYAIDLPNGGVAYIIGNVLQKGPRAEHRRMIAYGAEGIRHAVN
                     ELYLVNNTLVNDRGFGGVFVA"
BASE COUNT         1277 a         3052 c         3135 g         1117 t
ORIGIN      
        1 ggtcgcgtgg ccgcctgcga gtcgacgacg accaggtagc gcccgggcca ggggataggc
       61 tcgcggtcgg cgcgaagtac ccacaagacg tctccgcgcg ccagcgccgc gtcgtagtcg
      121 gcgtcgagga tgccgtggcg atccaggaag gtgttcagcg tggccgggat gcggatgcag
      181 cctttcgatt cggcgcgtcc cagccggggc tcgagccggt cggggtcggt ggcgtgcatc
      241 tgcaggcgca tcaagctggt cccgccgcgg ccccagccgc gctcggccgc gacccagccg
      301 aaatcgaaca cccgcatccc ggccaatcca tagccgcgga cgttgtgttc attgagggtg
      361 ccctcggcgc gaaagtccgg attgtccaaa gtgtgcgcga agactcccgt aggcgtgcgg
      421 aaatgctcga agccgcttgg ccggccggtc gataccggcg cggcgcccag ccagtgccag
      481 ccgcccgccg gcgtgcgcag gacgacgaag cccgcctgca cctgcgggct gcggtcgatc
      541 agcacgaagg cctgcgcccc gcggtcggac acgccggcct gagcgagcgc ctgcgcgagc
      601 atgacggcgt aacgctgctg gtcgttcggg ggcacgtcga gccggcggtc gacctcggcg
      661 gcgaagcgcg cgctccacgc cgccgcctcc ccgcccgcgc cgcgtgcgtc gatggcggag
      721 ccgcgggccg ggccgaacgc gaagaggctg gccaggtacg tgaagtccgc tggcgtcgat
      781 cccatcccga tcgtcaggat gagcgcgccc gccgaagcct tgagcagcgt cagcagcgtt
      841 tctgtcatgg cccgaatgcg tcgatgtctc aggatgatgg gcgccgctac ggtggcgccg
      901 ccggtagagc ttacaccgtg cacgcgtagc gcttggctgg ggccacgagg gcgcttcgga
      961 cttcacgcgc cgaacacgtc cgccagtggt cgtgacccgc accgggggct cccgaggtcc
     1021 gcgatcactc ctgactcagc cgccgaggta ggcccggcgc acgtggtcgt cggcagccag
     1081 gtcgcgcgcg gcgccggcca gggcgacgcg ccccgtctcc atcacgtagc cgcggtccgc
     1141 catgccgagc gcgaggtgcg cgttctgctc gaccatcagc accgtcgtgc cacggcggcg
     1201 gatgtcgtcg atgatggcga agacggcctc gacgatggtc ggggccaggc ccagcgaggg
     1261 ctcgtcgaag aggatcaagc gcgggcgggc catcagcgcg cgcgcgatcg ccagcatctg
     1321 ctgctcgccg cccgagagcg ttccggcggc ctgccgtcgt cgctgggcca ggatcgggaa
     1381 gtgctgcgac aggcgctcga cgtccctgac cacctcggcg cggtcgcggc gcacgtgggc
     1441 gcccatcatg aggttctcgt ggaccgtcat gtgggggaac acgcgccggc cttccgggca
     1501 gtgggcgatg ccggccgtga ggatgtccgc aggcgagtgc ccgtggatct cgcggccggc
     1561 gagcgtgatg cgcccgtgcg tggggcggag caggccggag atcgtccgca gggtggacgt
     1621 cttgccggcg ccgttggcgc cgatcaggca gaccagctcg ccctcgcgca cctcgagatc
     1681 gaggccacgg agcacttcga ccggcccgta ggcggcgcgc acgccctcga gctgcagcat
     1741 caggaccccg cgcgagccgg gtcgtcgcca gcccccgccg cgccggggcc gccggccggc
     1801 acccgcgtcg atccgaggta cgcgcggacg acctcgggat gcgactggat ggcggcgggc
     1861 ggcccctcgg cgatcaggcg accgccgctc agcacgatca ccgtgtcgga aatacccatc
     1921 accaaccgca tgtcgtgctc gaccacgaag acggtgacgc cctgatcgcg gatccgcctg
     1981 atcagcgcgc tggtcacggt cttctcggcg ctcgtcatgc cggcggcggg ctcatcgagc
     2041 agcagcatgc gcgggcgcgc ggccagcgcg acggccagct ccaccaaccg ctgctcgccg
     2101 tagggcagcg cgccggcggg ctcacgccgg cgcgcggcca ggcccacgaa ggcgaggatc
     2161 tcctcggcct cgacggccag cacgtgctcc tcgtcgcgga cccagcggcg tccggccagc
     2221 gcggcggccc agcccgcgcg gccccggagg tgcaggccga tcagcacgtt gtcgaggacg
     2281 gccagcgagg gaaacacact cgtcttctgg aaggtgcgga cgatcccccg gcgggcgata
     2341 gccgagggcc gcaggcgcgt gatcggccgg ccctcccaca gcacctcgcc ccgggtcggt
     2401 cgcaggtagc ccgtgatggc gttgaaggcc gtcgtcttgc cggcgccgtt ggggccgatg
     2461 agactcgtga ccgtgtcggg gcgcacctcg aagctcacgc cgccgagggc ctcgacgccg
     2521 ccgaacctca ccccgagatc gcggacggcg agggtcatgc cgaccggcgc gcgcgccagc
     2581 ccgcgagggc cggcacgatg ccgcggggca ggaagaacac gagcgcgacc agcagcacgc
     2641 cgtagagcac catctgccac tgccaggcaa cgacctcgcg cagcgcctcg ggcagcaccg
     2701 tgaacagcac ggcgccgacc agcggcccgg agagcgtgcc cttgccgccg gcgacgacca
     2761 tgatgaccat cgtgaccgtg tagctgaaga ggaagacctc ggggctgacc aggcgcgtgt
     2821 agtgcgcgta aaggctgccc gccatgcccg cgagggcggc gctggtcacc gccgcgacga
     2881 ccaggtagtg cacggcgtcg atgccgacgg actcggcgag cgcctcgttc tcgcgcagcg
     2941 ccaggaacgc gcgccccacg cgcgaccgca ccaggcggcg gcacacgagc gcggccagcg
     3001 ccgcgccggc gagcacgaga tagtagtacg cgctcttgct ccgaagggac cagccgaaca
     3061 gctcgggcgc cggcacgccg ggcaggccga gggggccgtt cgtcaggtcc atccagttca
     3121 cgctgacgag gctgatcacg cccgcgaacg agatcgtcac cagcacgaag tacgcgccac
     3181 gcaccttgag cgccaggcgg ccgatggccc atcccgccag cccggcggcc accgtgccgc
     3241 aggcgagccc cgcccagaac gaccactcga gccgcagcgt gagcagcgcc gaggtgtagg
     3301 caccgatgcc gaagaacgcc gcgtggccca aggagagctg gccggcgtag ccgagcagaa
     3361 ggttcaggct cgacgcgagg atcacgaaga tgccggccat gatgatcacg tgcaggtggt
     3421 aggggctctg cacccagagc ggcacggtga ggaccacgat ggcgccgagc gccgggagca
     3481 gccgtctcat ccgccgaggt gagcacgggc ggggtggtga gcgcgcgtcg cccggtgggc
     3541 gtgcgcggcc cggagcaatg cctcatcggg cccactgggt gggccggcgc cggaggcgaa
     3601 cgccctcatc cgatgcgctc cgcgcgcgcg aagagtcccg acgggcgcag gagcaggacg
     3661 ccgatgatca tcacgaagcc cacggcgtcg cggtagcccg acgacacgta gccggcgccc
     3721 agttcctcgg cgatgccgag gatgagcccg ccgagcgtcg cgcccggcag gttgccgagg
     3781 ccgcccagga tgaccacgca gaaggcgcgg agggcggcga ggtcgcccat cgacggatac
     3841 gcgaggaaga ccggcccgag cagcgccccg gcggcgctgg ccagcccggc gccgaacgcg
     3901 aaggtcgcgg tgtggacgcg atggatgcgc acgcccatga gctgggcggt gtcggggtcc
     3961 tggaaggtcg cgcgcatggc ctggcccagc cgcgtgcggt ggatgaccag gtaggacccg
     4021 gcgatcagcg ctgcggcgac gcccaggacc accagacgca gcggcgcgat cgagaccggc
     4081 ccggccacga tgggctcggt ggggaacggg tgagggatgg acttcgccac gccgccgaaa
     4141 ccgaggagga ccgcgttctg cagtgcgatc gacacgccga tcatgacgag catggtcgtg
     4201 tcgatcgact cgccgtgcag cggccgcagc agcacgcgct cgcaggcggc gccggcggcg
     4261 gccccgacga gcagcgccgc ggccacggcc gcgaagaagc tgacctccgc gagcgccagc
     4321 gcggtgaacg ccgcgtacgc ccccagcgtg tacagctcgc cgtgggcgaa gttcaccacg
     4381 ttcatcaggc cgaagatcag cgtgagcccg atgcccagga gggcatacgt gccgccgagc
     4441 accaggccgt tcaccaggtg ctggagcagc tgctccacgg tgcggggccg gacgctactt
     4501 ccgggcgacg aagttgggca gggccacctt cccgtccttg atctggacga tgaagatgct
     4561 cggccgggac tggccgctct ccttgccggc gggcccgtcc ttcgcgaact tgatggggcc
     4621 gttgacaccc atcaggctca ccttccacag cgcctcgcgg agggccttgg ggtcgtcctt
     4681 gccggccacc ttgacggcct cggcgatggt cgcgatgccg tcgtggccgc ggaagccctc
     4741 ggtgaggccc tcgaacgggt gaccgcgctt cgcccactcg tccacgaagg ccttggcgag
     4801 cttgccgtcc ggcatcgcct ccgggaacca cggcatgaag aacacgatgt ggtaactgcc
     4861 ctcggcggcg ggaccggcct gcttgatgag ctgggtcggc gacgaggagc cgccggtcgt
     4921 gatgatcttc cgtccgaggc gctgctcctg tgactgcttg agcacgagcg tgatctgctc
     4981 caccgcagtg gtgaggaaga gcgtgtcgcc gccggtaccc ttgaccttgg tgatctgggc
     5041 gttcatgtcc gtcgccgcct ggtccatgaa ctccgtgacg ccgacggtga cgttgtgctt
     5101 cttgagcacg tcgccgaagg ccgtgatcgc cccgcgaccc cagtccgtgt tcacggccag
     5161 aaagtcggcc ttcttgatcc ccagcggcgc gaggtacttc tcgagcccca gcgcttccat
     5221 ctccgacggc gggctgatgc ggaagatcca cgggttgccg cgcctggtga tggaggccgc
     5281 gctggaggtc tcgaccacca tcggcacgcc gtactcctcg agcttcggca tcgccgccag
     5341 cgtcatcgac gagccccagg cgcccatgat ggctggcacc ttgtcgcgga cgatcagctt
     5401 ctcggccgcg ctggccgcct ccttggggtc ggacttgttg tcctcgatga cgagctcgac
     5461 cttgcggccg ccgacgccac ccttcgcgtt gatccagtcg cgggcgatct cggcgcccat
     5521 gcggacgtag ttgccggagg cggcgaccgg gccggagagg ggctggatga cgccgatctt
     5581 gatcggcgcc tgcgattcgg cgacgccggc gaccaggagc gcgacgcctg cgacgactgt
     5641 caggggcacg gcccagcgct tgcgcatcgg agacctccag ggtcgggttg tggcagggag
     5701 gatagcgcag gagcgggcgg cgcgaaagcc gctgccgcct acgcgacccc ggtgagcacg
     5761 tcgagcaccg cgggccgccg ctcctccttg accacgcgca gcgcccgctt gagcgcgggc
     5821 ccgagcgccg cggcgtcgtc gatcggaccc tcggcgtgga tgccgaaggc cgcggcgatc
     5881 cgcccgtagt ccggcgcggg gtcctcgatg cggatgccga ccgtcttgcg ctcgaccggc
     5941 cggccccgct ctcgcgcgac cgcctcctgg tgcatctcgt cgttgtagta cgtgccgttg
     6001 ttgcagatga ccgtcagcag cggcagccgg tgatgcgcgg ccgtgtagaa cgcgctcgcc
     6061 acgtagagca ggtcgccctc cgactggagg ttgacgcaca gccggctcgt gttccggaac
     6121 gccagcgcca tgccgagcga ggccggcagg ccgtagccga gtccggcccc gccgctgccg
     6181 ccgaaggcgc gctccggctg ccagtcccag aggcggcgcg cccagccgcc cgcgacgccg
     6241 ttcgtcagca cccagtcctc gcccgcgatg gcggcccaca cctccgccgc gagcaccggg
     6301 agggccaggg gccgcgccgt ccgccgggca tcgacggccg cccgccactc gccctggcgc
     6361 gccgcatggc cgcgccggca ccgctcgagc cgcgcctgcc gctccggggc gcgccgatcg
     6421 tgccgcaacc ggtcttccac gagcgcgtcg agcgtgggca gcgcgacggc ggtgtcggcg
     6481 gcgatcggca cgtccactgg caccagcgac tggaaggtgt gggcccagct ccggaacgcg
     6541 tagtcgttca gcgagatgct gatgagcttc atcgagggcg cggccagcga gcggacccgg
     6601 cgagtggcgc gatccgtggt cccgagcgcg ccgaggtagt cctcgacatc gagggccagg
     6661 atgacgtcgg cctcgcgcag ggcattctcc cgctcgtcgg tggcgtcgag cgggtgcgat
     6721 ccggggaagc tgggccgccc gcgagacgac tggctcgtgt cgacgaccgg cgccgccagc
     6781 cgctcggcga gccggcgaag tgccgccaga gcctccgggg ccctcgccag cgcctccacc
     6841 acgatcaccg gtgaggccgc ctccgccagg agccgtgccg ccgtctcgag cgcccggccc
     6901 tcggcctgcg gtggcgccgg cggcgcgaag cggccggggt ccggaatcgc cattggcccg
     6961 gtcaacgctt cctcctgcag cgcggcgtcg aggcagaggt agacggggcc gcgcggcggc
     7021 gtcgaggcca gccgccacgc gcgcgtgaac gcctccggaa tggcggcgat gctcgccggc
     7081 tggtcgtccc acttgacgta gtcgcgcacc tgctggccct gcacgagtgc ggtgtggatc
     7141 cagtcgatcc acggccggcg cttcgtcgcg tccatgggac cggtggcacc gacgacgaag
     7201 accggcgcgc ggtcacagaa ggcgttgaag atcgccgagc acgcgtgctg gagcccgacg
     7261 acatcgtgga cggcggccgc catcagcttc cccgtggcct tggcgtagcc gtgggccagc
     7321 gccacggcga tctcctcgtg gcagcagagg atgagctccg gccgggtgtt gccgccgtag
     7381 ttgacgagtg agtcgtggag cccccggaac gtcgcaccgg gattcagcgc gacgtactcg
     7441 atgccgaacg cgcgcatcag atcgaccacg acgtcggaac cgtactcggc cctggccaac
     7501 ggcgcctcct cccagatcat gagagggagc ccgacgggcc cctcccgacc aacgctctcg
     7561 ccatgtccgg tgcggcgtcg ccgacgccga gcccggctgc cgtccgcata cagtgccggc
     7621 ggtgcgcgcg tcaagccgga aagagtgggg cgcgcgggcg gtatagtcgg ggcgtggagg
     7681 aggacgctcg tgaacgtgca aacgagagcc ccggccggcc gtggcgtggt cgcgctgggt
     7741 ctgctggcca tgtgtcccct ggcgacgagc gagcacgtgg ccgccggtga tccaagcacc
     7801 gccgggcgcg tcctgcgcgt cgggccgggc cgtgagctgg cgcgtcccag ccatgccgcg
     7861 gccctggcgc gcgccggcga caccgtggag atcgacgccg agctgtacga gggcgatgtc
     7921 gtcacgtggc gggctgacca cctcaccctt cgcggcgtgg gcggccgggc acggctgcga
     7981 gccgccgggc aggccgccga ggccaaggcc atctgggtca tacggggccg gaacaccgtg
     8041 gtcgaggacg tggagttctc cggcgcgcga gtgcccgacc ggaacggcgc cggtatccgc
     8101 caggagggcg ccgacctgac catccgccgc tgcctcttcc acgacaacga gaacggcgtc
     8161 ctcctcgggg gtggcccgga cagcgagacc gtcatcgagg agtcggagtt cgcggccaac
     8221 ggcgctggcg atggccgcag ccacaacatc tacatcgtcg ccgtccggcg cttcgtcctt
     8281 cgcggcagcg acgtgcacca cgcccggacc gggcacaacg tcaagagccg ggcgcgcgag
     8341 aacgtcatcg tcggcaaccg catcgtggac gaggccaccg gcacgtcgag ctacgcgatc
     8401 gatctcccga acgggggcgt ggcgtacatc atcggcaacg tcctccagaa gggcccgcgt
     8461 gccgagcacc gccggatgat cgcttacggc gccgagggga tccggcacgc cgtgaacgag
     8521 ctgtacctgg tcaacaacac gctggtcaac gatcgaggct tcggcggggt gttcgtcgcc
     8581 g
//
LOCUS       DATIAZ010000096        11354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_418689, whole genome shotgun sequence.
ACCESSION   DATIAZ010000096 DATIAZ010000000
VERSION     DATIAZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11354
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418689"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VLF19_03300"
     CDS             <1..244
                     /locus_tag="VLF19_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92306.1"
                     /translation="RSQTAVMTIAPGADAGPEEEHEGDQIVFVIEGEAVVRVADREYR
                     AGPGALLVVPAKTRHHVRNSGPTPLFFVTVYAPPAY"
     gene            complement(274..669)
                     /locus_tag="VLF19_03305"
     CDS             complement(274..669)
                     /locus_tag="VLF19_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92307.1"
                     /translation="MKKTVAMLMLATPLTFALPSPAAAGASEDAALALGAFAVFNQLV
                     RGETVLHGLFGPPRPVVVQQPPVVYAPPAPPPVVYAPAPVVVYAPRPPVVYAPPPAVI
                     VRPGGYWAYRGVHRVWVAHPAKHRHWKHR"
     gene            826..1743
                     /locus_tag="VLF19_03310"
     CDS             826..1743
                     /locus_tag="VLF19_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSE92308.1"
                     /translation="MGEARGQIGRLEAAIGRSVVGKPEAVRLAVVGLLARGHLLIEDA
                     PGVGKTTLAAALARAIGGRFQRIQFTSDMLPADVLGVTVWQPDRGDFLFKPGPLFANV
                     VLADEINRTTPKTQSSLLEAMNEAQVSIDHATHPLPRPFIVLATQNPREFEGTYPLPE
                     SQLDRFLLRIKIGYPQAADEKAILRGASAPPDTLEPVLDESTVLGLQAETEQVRAGDA
                     VLDYLMTLVSATRQSALLSLGVSPRGSLGLLGAARAAALADGRDWVVPDDVKRLAVAA
                     LAHRVIPRAGGLDGEAALRAILQEVPVPR"
     gene            1743..2729
                     /locus_tag="VLF19_03315"
     CDS             1743..2729
                     /locus_tag="VLF19_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSE92309.1"
                     /translation="MSPEWPPAFLRPKRSIQPTREGWWCLAVALLLGLTAVNSGNNLL
                     YLLDSLLLALVIVSGVMSEQSLRGVRLTPLVPDEVFAGQAVMLGAVVRNAKRRLPARS
                     IALETADGGRVISLPWLGPGAELVAAWSHAFPRRGPQQLPGLRLTTRFPFGLFAKRGR
                     VQFEQEVVVFPAPVATAARRLGIAPAGGGATARRRGRGQGLHDLRDYRPGDDPRLIHW
                     RVSARTGTTVVRELEAEATADVRILLRGTGARDPQRLERGLSEAAGLVLHGLAGGAAV
                     SLAGPGLAVPPGRGRDHARRVLTALALYTPPQASSAGASRPASGRDVVVELD"
     gene            2731..4743
                     /locus_tag="VLF19_03320"
     CDS             2731..4743
                     /locus_tag="VLF19_03320"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HSE92310.1"
                     /translation="MRTLSALRLATTLAVGAGVVALRLGGLLGAAGLAVVLLALAGGW
                     WLRERVTPSRGLTRALTAILALAVTVDVLLVAAVAFDGFVRLLLVLTLLRLLTARRPR
                     DLRDAGMLAFFMPVAASAVTMSVDFLFVLVAYVIAAVTMLVIAHDLGEAERAGAGPTA
                     LAELRTGRGAAVIGLAGAAVTFGVTAALFFVIPRVGEATLALRGQEKKMLIGFTDRVE
                     LGAIGQLENDATVAMRVQLPGGAPAPALLPHLRWRGITLDHYDGLAWSEIRQQRRLII
                     QHGPEGPVEVQPPRGAGAYLRQAVFLEPIGSDAVFAAPHALRFWARGGAALVNDAGVI
                     TVPSPSARLQYTVDSVVGPGPWGDPLGAADAARYLQLPPLPPRIAALAREVTAGVRSQ
                     AAAAAALTAFLSREFHYTLKLERRTVLDPLEEFLFVRRSGNCEYFAAALAVMLRTLGV
                     PTRIVNGFQRGDWNPYGGYFLVRMGDAHSWVEAHVDGTGWITLDPSPRGTPDADAARA
                     AALWVDALRVRWYRYVVGWSRQDQVQAAVALQRAAARPWRLPWRGLTVPAWGAVPALA
                     AVAAAVGWLGWRRMGCSGPSARPVRPPDFYRRALRALARRGLRPAPAETAREFASRVA
                     ATVPPAADAFAHITRAYETVRFGDRALDAATTHAVHAALAALREAR"
     gene            complement(5124..5690)
                     /gene="pyrE"
                     /locus_tag="VLF19_03325"
     CDS             complement(5124..5690)
                     /gene="pyrE"
                     /locus_tag="VLF19_03325"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSE92311.1"
                     /translation="MTERETLALYEKTGALMHGHFRLTSGLHSDVYLQSALVLQHPEH
                     AAALGEALAEAFRDVAATTVLAPAIGGILVAHEVASALGVRALFTERENGVMSLRRGF
                     ALARGEHCLVVEDVITTGGSTREVIECVERHGATVVGVGSLIDRSGGTAAFAVRRAAL
                     ATVDATTWKPEACPLCASGGEAIKPGSR"
     gene            complement(5687..6508)
                     /gene="pyrF"
                     /locus_tag="VLF19_03330"
     CDS             complement(5687..6508)
                     /gene="pyrF"
                     /locus_tag="VLF19_03330"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSE92312.1"
                     /translation="MTPVDRVLVAIDVDGLAEATRLLDRLAGVVAGCKIGNQLFTAAG
                     PAAVEAARKRGFRVFLDLKFHDIPNTVAGAVREAARLGVFMLNVHASGGRAMMRAAAE
                     AAAKAAAEFGLPRPICLGVTVLTSLDRRALDGELGVAATVEAHVLHLARLAREAGLDG
                     CVASPREIAPLRINLGNKWVIVTPGIRPAATHAGRLGGHVAAPGPDNPDAAPLRETGG
                     VAGRVGAPRSDDDQLRIATPRAALTAGADYLVVGRPITAAADPAAAARAILEELG"
     gene            complement(6495..9698)
                     /gene="carB"
                     /locus_tag="VLF19_03335"
     CDS             complement(6495..9698)
                     /gene="carB"
                     /locus_tag="VLF19_03335"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSE92313.1"
                     /translation="MPKRTDIRKVLIIGSGPIVIGQACEFDYSGTQGCRALREEGFEV
                     VLVNSNPATIMTDPELATRTYVEPLTPEFVGKVIARERPDALLPTLGGQTGLNLAVAL
                     AEDGTLDRLGVELIGAKLPAIRKAEDRALFKQTIEALGLALPRSHQVTSLDEARRAAT
                     ELGFPLVLRPSFTLGGSGGAIAHTAADLEPAVRWALAQSPVGQVLVEESVLGWKEFEL
                     EVMRDLADNVVVICSIENLDPMGVHTGDSITVAPAQTLTDKEYQLMRDAAAAIIRAIG
                     VETGGSNIQFAVNPRDGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGCTLDE
                     LRNDITRETPASFEPVLDYCVVKIPRWAFEKFPEADPTLTTQMKSVGEVMAIGRTFKE
                     ALHKAVRSLEQDRWGLTLGRTVSAEELERLLRVPTPDRLFALGDAFRAGMTVERAHAL
                     TAIDPWFLDQIRQLVAFEAAIQRAGLDDAHVLKRAKQWGFADRRLAELTGTSERRVRE
                     TRLQYGIRATFKTVDTCAAEFVAHTPYLYSTYEDEDEAPPTARKKVVILGSGPNRIGQ
                     GIEFDYCCVHAAFALREAGVEAIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLNIVDK
                     ERPFGVVVQLGGQTPLKLAVPLQQAGVPILGTSPDAIDRAEDRQRFSALLDELGLLQA
                     AGATARSLAEAQQIAPALGYPVLVRPSYVLGGRAMQIVHDAEDLRTYVSEAVRVSPEH
                     PILVDKFLEDAIEIDVDAVSDGRQVLVGGVMEHIEKAGIHSGDAACALPPYSLGDDQV
                     RNLREQTRALARALGVVGLLNIQFAVKNDLVYVLEVNPRGSRTVPFVSKAIGLPLAKL
                     ATRALLGEPLGELAALDPEPPYISVKEAVFPFVKFPGVDAVLGPEMKSTGEVMGLDRD
                     FRKAYLKSQLAAGSTLPTSGKVFISVKNADKRGVVMLAKRLVEMGFGLVATGGTARVL
                     QRHGMTVDVVNKVGDRWRPNVIDLMQRGEIALVFNTPEDGRARKDSAEIRRTAVAQQI
                     PYYLTVDGIQAAIGAIEVLLKEEPTVTSLQEYHVDAR"
     gene            complement(9705..9971)
                     /locus_tag="VLF19_03340"
     CDS             complement(9705..9971)
                     /locus_tag="VLF19_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92314.1"
                     /translation="MPLYLISYRKTEGPGHKPEWASFTTQSDASLEAHAVRDRVAKRM
                     SVLGEQLWGNGEAVWIGGGRLDDVLYRREEATPETSIVYGLVEE"
     gene            complement(10000..11211)
                     /gene="carA"
                     /locus_tag="VLF19_03345"
     CDS             complement(10000..11211)
                     /gene="carA"
                     /locus_tag="VLF19_03345"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSE92315.1"
                     /translation="MTDALLVLRDGRVFRGEALGAIGEAGGEVIFNTAMSGYQEILTD
                     PSYRGQIVAMTYPLIGNYGVNTQDVESRRPWVNAFIVREGALRPSSWRAQVPLDEYLR
                     THGIVGLQGIDTRALTRHLRDHGAQDGIVSSTDLDVDRLRERARALPGLVGRDLVGEV
                     TCVAPYTWHDEGTWRLGAGYQPPAPPRFGVVAYDAGIKLNILRQLAASGCAVTVVPAA
                     TPVAAVLERRPDGVVLSNGPGDPEGVPYLIDAVRGLLGRVPVFGICLGHQILGLAAGG
                     RTYKLPFGHHGANHPVKDLPTGRVEITSQNHGFAVDPASVERAGWEPTHVNLNDGTCE
                     GLRHREWPAFSVQYHPEASPGPHDASYLFERFTDLMGMGGSGRPPSLPGAPGAPAQPG
                     RPSTTPFGSRT"
BASE COUNT         1523 a         3992 c         4196 g         1643 t
ORIGIN      
        1 gcgcagccag acggccgtga tgacgatcgc cccgggcgcc gacgcgggcc cggaggaaga
       61 gcatgagggc gatcagatcg tgttcgtgat cgaaggagag gccgtcgtgc gtgtggccga
      121 tcgcgagtat cgcgcgggac ccggtgcgct gctcgtcgtc cccgccaaga cccgacacca
      181 cgtcaggaac tccggtccca cgccgctctt cttcgtcacc gtgtacgcgc cgccggctta
      241 ctagagtcct cttgcgaggc cctctcccgc cgcttaccgg tgcttccagt gacgatgctt
      301 ggcggggtga gccacccaca cccggtgaac accgcggtac gcccaatagc cgccgggacg
      361 gacgatcacg gcgggcggcg gcgcgtacac gaccgggggc cgaggcgcgt agacgacgac
      421 cggcgccggc gcgtagacca caggcggcgg agcgggcggc gcatagacca ccgggggttg
      481 ctgcacaacg acggggcgag gaggaccgaa caggccgtga agcacggtct cgccgcgcac
      541 gagctgattg aagacggcga atgcgccgag cgccagggcg gcgtcctcgg aagctccggc
      601 agcggccggg gacggcagag cgaacgtcaa gggtgtggcc agcatcagca tggcgaccgt
      661 cttcttcatg ggcaaccccc tcgctcggcg tgttgaactt cctgcccatt ggacgccagg
      721 gctcccggcg ctattcgggg gccgtggaac gtcggacgat tgcaggcttt tccggacccg
      781 cccgcgatcc ttgtcaaaac gtcgccgagg ctattagatg ggcgggtggg ggaggcacgc
      841 gggcagatcg gccggctcga ggccgccatc ggccgctcgg tggtcggcaa gccggaggcg
      901 gtccggctcg ccgtcgtcgg gttgctggca cgcggccacc tcctgatcga ggacgcgccg
      961 ggcgtgggaa agacgacgct cgcggccgcg ctggcgcgtg ccatcggcgg tcgcttccag
     1021 cgcatccagt tcacctcgga catgctgccg gccgacgtcc tcggggtgac ggtgtggcag
     1081 cccgaccgtg gcgacttcct gttcaagccc ggtccgctgt tcgccaacgt cgtgctcgcg
     1141 gacgagatca accgcacgac gccgaagacc cagtcgagcc tgctagaggc catgaacgag
     1201 gcccaggtgt ccatcgacca cgcgacgcat ccgctcccac gtccgttcat cgtgctggcg
     1261 acgcagaacc cgcgcgagtt cgagggcacg tatccgctgc cggagtcgca gctcgaccgc
     1321 ttcctgctgc ggatcaagat cggctacccg caggccgccg acgagaaggc gatcttgcgc
     1381 ggcgcctcgg cgccacccga cacgctcgag ccggttctcg acgagagcac ggtgctgggc
     1441 ctgcaggccg agaccgagca ggttcgcgcc ggcgacgccg tcctcgacta cctgatgacg
     1501 ctcgtcagcg ccactcggca gtcggccctg ctgtcgctcg gcgtgagccc gcgcggctcg
     1561 ctcgggctgc tgggcgccgc ccgcgccgcg gccctggccg acggccgcga ctgggtcgtg
     1621 cccgacgacg tcaagcgcct cgccgtggcg gccctcgcgc accgcgtgat cccgcgcgcc
     1681 ggcggcctgg acggtgaggc ggcgctccgt gcgatcctcc aggaggtccc ggtcccgcgc
     1741 tgatgtcacc ggagtggcca cccgcgtttc tccggccgaa gcgcagcatt cagcccaccc
     1801 gcgagggctg gtggtgcctg gccgtcgcgc tcctgctcgg gctcaccgcc gtcaacagcg
     1861 gcaataacct cctctacctg ctggactcgc tgctgctggc gctggtgatc gtttccggcg
     1921 tgatgtccga gcagtcgctt cgcggcgtgc gtctgacccc gctggtaccc gacgaggtgt
     1981 tcgccggtca ggcggtgatg ctgggggcgg tcgtgcggaa cgcgaagcgg cgcttgccgg
     2041 cgcgctcgat cgcgctggag acggccgacg gcgggcgcgt gatctcgctg ccatggctcg
     2101 gtccgggcgc cgagctggtc gcggcgtggt cgcacgcgtt cccccgccgt ggcccgcagc
     2161 agcttcccgg gctcaggctc accacgcgct ttcctttcgg gctcttcgcc aagcgcgggc
     2221 gtgtacagtt cgagcaggag gtggtcgtgt tccccgcgcc cgtggccacg gcggcccggc
     2281 gtctgggtat cgcaccggcg gggggcggcg ccacggcccg ccggcgggga cgtggtcagg
     2341 gccttcacga cctgcgcgac taccggcccg gggacgatcc ccgcctgatc cactggcggg
     2401 tcagcgcgcg gaccggcacc acggtggtgc gcgagctcga ggccgaggcg accgccgacg
     2461 tccgcatcct gctgcggggg acgggcgcgc gcgacccgca acgcctcgag cgtggcctct
     2521 ccgaagccgc cgggctcgtc ctgcacggtc tcgcgggggg cgctgcggtg tcgctcgccg
     2581 gccctggcct tgccgtgcct ccgggccgcg gtcgcgacca cgcgcgccgc gtcctcaccg
     2641 cgctcgcgct ctacacgccg ccgcaggcgt cctccgccgg tgcctcgcgg ccggcgtcgg
     2701 gacgcgacgt ggtcgtggag ctcgactgag gtgcggacgc tctccgccct ccggctggcc
     2761 acgaccctcg ccgtcggcgc gggcgtcgtc gcgctgcgcc tgggcggcct cctcggcgct
     2821 gccggcctcg ccgtcgtgct gctcgcgctc gcaggcgggt ggtggctccg cgagcgcgtg
     2881 acgccctcgc gcggtctgac gcgagcgctg acggcgattc tcgcgctcgc cgtcaccgtg
     2941 gacgtgctgc tcgtcgcggc cgtcgccttc gacggcttcg tccgcctgct cctcgtgctc
     3001 acgctgctcc ggctcctcac cgcccggcgc ccgagggatc tccgcgacgc cggcatgctc
     3061 gcgttcttca tgccggtggc ggcgagcgcg gtcacgatgt ccgtggactt cctcttcgtt
     3121 ctcgtcgcgt acgtgatcgc cgcggtgacg atgctcgtga tcgcccacga cctcggcgag
     3181 gcggagcgcg ccggcgccgg cccgaccgcg ctcgccgagc tgcggaccgg acgcggcgcg
     3241 gcggtcatcg ggctcgccgg cgccgccgtg acgttcggcg tgacggccgc actcttcttc
     3301 gtgatcccgc gggtaggcga ggcgacgctc gcgctgcgcg gccaggagaa gaagatgctg
     3361 atcgggttca cggaccgcgt cgagctcggc gccatcggcc agctcgagaa cgacgccacc
     3421 gtggccatgc gcgtccagct gccgggcggc gcgccggcgc ccgcgctcct gccgcacctc
     3481 aggtggcgcg ggatcacgct cgatcactac gacggcctcg cctggagcga gattcgccag
     3541 cagcgccggc tgatcatcca gcatggtccc gaggggcccg tcgaggtgca gccgccgcga
     3601 ggcgccggcg cctacctgcg ccaggccgtc ttcctcgagc cgatcggcag cgacgccgtg
     3661 ttcgccgccc ctcacgcgct caggttctgg gcgcgcggtg gcgccgcgct ggtgaacgac
     3721 gcgggcgtga tcaccgtgcc gtcgccatcg gcgcggctgc agtacaccgt ggactcggtc
     3781 gtcggccccg gcccctgggg ggacccgctc ggcgccgcgg acgctgcgcg ctacctgcag
     3841 ctgccgccgt tgccgccgcg catcgccgcg ctggctcggg aggtcactgc cggcgtgcgc
     3901 agccaggctg cggcggcggc ggcgctgacg gccttcctgt cgcgggagtt ccactacacg
     3961 ctgaagctcg agcgccggac cgtcctcgac ccgctggagg agttcctctt cgtgcggcgc
     4021 agcggcaact gcgagtactt cgcggcggcg ctcgccgtca tgctgcgcac gctcggcgtg
     4081 cccacgcgga tcgtgaacgg cttccagcgc ggagactgga acccgtacgg cggatacttc
     4141 ctggtccgga tgggggacgc gcactcgtgg gtggaggcgc acgtggacgg caccggatgg
     4201 atcacgctcg acccatctcc acgcggcacg cccgacgccg acgcggcgcg ggccgcggcg
     4261 ctctgggtcg atgctttgcg cgtacgctgg taccgctacg tcgtcggctg gagccgccag
     4321 gatcaggtgc aggcggccgt ggcgctgcag cgggcggcgg cgcgaccctg gcgcttgcca
     4381 tggcgcggcc tgaccgtgcc cgcctggggc gcggtgccgg cgctggccgc cgtggcggcc
     4441 gccgtcggct ggctcgggtg gcgccgcatg gggtgctccg gcccatccgc gcggcccgtg
     4501 cgcccaccag atttctaccg ccgcgccctc cgcgcgctgg cccgccgcgg cctccgcccg
     4561 gctcccgccg agaccgcccg cgagttcgcc tctcgcgtcg ccgccaccgt cccaccggct
     4621 gctgacgcgt tcgcccacat cacgcgtgcc tacgagaccg tccgcttcgg cgaccgggcc
     4681 ctcgacgcgg cgaccacgca cgccgtccac gccgctctcg ccgccctccg cgaagctcgc
     4741 tgacccgcgc accgcgggag ccgggccatg tccaccgtca gcgcaggctt ggcccgcgca
     4801 atagggtcgc cgtcggtttc ggaccagatc tgagcatcca ggctctagag tctcctgaat
     4861 cgcaagttcg ccgaccgtag gccgggatgg gtccggggcg cgggtccgcc gacctgtctc
     4921 cgccacggac cccgcgcagc ccgaaggggg aggcagcaac accagaatgg cgacgcgcag
     4981 cgcatcaggg gtcgccggac ccgcgccccc gaccccgtcc cggccggcgc gtcagcagcg
     5041 aactcctgat tcgtgacact ccggaaggca agagtgcacc ccgaggcgcg gacgacccgt
     5101 cggaggggcc ggccccctcc gagtcaccga ctcccgggct tgatcgcctc accgcccgat
     5161 gcgcacagcg ggcacgcctc gggcttccag gtcgtggcgt cgacggtggc cagcgccgct
     5221 cgccggaccg cgaacgccgc ggtcccgccg ctccggtcga tcagcgagcc gaccccgacg
     5281 accgtcgcgc cgtgacgctc cacgcactcg atcacctcgc gcgtcgagcc acccgtggtg
     5341 atgacgtctt cgaccacgag gcagtgctcg ccgcgcgcca gcgcgaagcc gcgccggagg
     5401 ctcatcacgc cgttttcgcg ctcggtgaac agcgcgcgca ccccgagtgc gctcgcgacc
     5461 tcgtgggcga cgaggatacc gccgatagcc ggcgcgagca ccgtggtggc ggccacgtcg
     5521 cggaacgctt cggccaacgc ctctcccagc gcagcggcgt gctcgggatg ctggagcacc
     5581 agcgcggact ggaggtagac gtccgagtgc agtcccgagg tgagccggaa gtggccgtgc
     5641 atcagcgcgc cggtcttctc gtagagcgcg agggtctcgc gttcggtcat ccgagctcct
     5701 ccaggatcgc gcgcgcggcc gcggcggggt cggcggcagc ggtgatcggg cgccccacga
     5761 cgaggtagtc cgccccggcg gtcagcgccg cgcgcggtgt cgcgatgcga agctggtcgt
     5821 cgtcggatcg gggagctccg acacggcccg ccaccccccc cgtctcgcgc agcggtgccg
     5881 cgtccggatt gtccggcccg ggagcggcga catggccgcc gagacgtccc gcgtgcgttg
     5941 cggccggccg gatgcccggc gtcacgatga cccacttgtt gccgaggttg atccgcagcg
     6001 gcgcgatctc gcgcggcgag gcgacgcaac cgtccagacc cgcctcgcgc gccagccggg
     6061 cgagatgcag cacgtgcgcc tcgacggtcg ccgccacgcc cagctcgccg tcgagggcgc
     6121 ggcggtcgag gctcgtgagc acggtcacgc cgagacagat cggccgcggg aggccgaact
     6181 ccgccgccgc tttcgcggcc gcctccgcag ccgcgcgcat catggcgcgg ccgcccgaag
     6241 cgtgcacgtt gagcatgaac acgccgagcc gtgcggcctc gcggaccgcg ccggcgaccg
     6301 tgttgggaat gtcgtggaat ttcaggtcca ggaagacgcg gaacccgcgc ttgcgcgctg
     6361 cctcgacggc ggccggcccc gccgcggtga agagctggtt gccgatcttg cagccggcga
     6421 ccacgccggc gagccggtcg aggaggcgcg tggcctcggc cagcccgtcc acatcgatgg
     6481 cgacgagcac ccggtcaacg ggcgtcaacg tgatactcct gcaggctcgt caccgtgggc
     6541 tcctccttga gcaggacctc gatcgccccg atcgccgcct ggatcccgtc gacggtgagg
     6601 tagtagggga tctgctgggc caccgccgtg cggcggatct ccgcggagtc cttgcgggcg
     6661 cggccgtcct ccggtgtgtt gaatacgagc gcgatctcgc cgcgctgcat gaggtcgatc
     6721 acgttcggcc gccaccggtc gccgaccttg ttgaccacgt cgaccgtcat gccgtgccgc
     6781 tgcagcacgc gcgcggtgcc gcccgtggcc acgagcccga accccatctc gacgaggcgc
     6841 ttggccagca tcaccacgcc gcgcttgtcc gcgttcttga cggagatgaa caccttgccc
     6901 gaggtgggca gggtggagcc ggcagcgagc tggctcttga ggtacgcctt gcggaagtca
     6961 cggtcgaggc ccatgacctc gccggtagat ttcatctcgg ggccgagcac ggcgtccacg
     7021 ccggggaact tgacgaaggg gaagacggcc tccttgacgg agatgtacgg cggctcggga
     7081 tcgagggcgg cgagctcccc gaggggctca ccgagcagag cacgggtcgc gagcttggcg
     7141 agcggcaggc cgatggcctt ggacacgaag ggcacggtgc gggagcctcg cgggttgacc
     7201 tccagcacgt agacgaggtc gttcttcacc gcgaactgga tgttgagcag gcccacgacg
     7261 ccgagggcgc gggccagggc gcgggtctgc tcgcgcaggt tgcggacctg gtcgtcaccg
     7321 agcgagtagg gtggcagcgc gcacgcggcg tcgccggagt ggatgccggc cttctcgatg
     7381 tgctccatga cgccgccgac gaggacctgg cggccgtcgc tgacggcgtc cacgtcgatc
     7441 tcgatggcgt cctcgaggaa cttgtcgacg aggatcggat gctcggggga gacgcggacg
     7501 gcctcggaca cataggtgcg gaggtcctcg gcgtcgtgga cgatctgcat ggcgcggccc
     7561 ccgagcacgt aggagggccg caccagcacg gggtagccga gcgcgggagc gatctgctgg
     7621 gcctcggcca gggagcgggc ggtggcgcca gcggcctgga gcaggccaag ctcgtcgagc
     7681 agggcgctga agcgctggcg gtcctcggcg cggtcgatgg cgtcggggga ggtgccgagg
     7741 atgggcacgc cggcctgctg cagggggacg gcgagcttga ggggggtctg gccgccgagc
     7801 tggacgacga cgccgaaggg gcgttccttg tccacgatgt tgaggacgtc ttcgagcgtc
     7861 agcggctcga agtacagccg atcggacgtg tcgtagtcgg tggaaaccgt ctcggggttg
     7921 caattgacca tgatggcttc cacgccggcc tcgcgcaggg cgaaggcggc gtggacgcag
     7981 cagtagtcga actcgatgcc ctggccgata cggttggggc cggaaccgag gatgacgacc
     8041 ttcttgcggg cggtcggggg ggcctcgtcc tcgtcctcat aggtggagta gaggtacggg
     8101 gtgtgggcga cgaactcggc ggcgcaggtg tccaccgtct tgaaggtggc gcggatgcca
     8161 tactgcaggc gcgtctcccg cacgcggcgc tcggaggtcc cggtcagctc tgccaggcgc
     8221 cggtcggcga agccccactg cttggcgcgc ttgagcacgt gcgcatcgtc gagtccggcg
     8281 cgctggatcg cggcctcgaa ggcgacgagc tggcggatct ggtcgaggaa ccaggggtcg
     8341 atggcggtca gggcgtgcgc tcgctcgacc gtcatgccgg cccggaacgc gtcgccgagc
     8401 gcgaagagcc ggtcgggggt gggcacgcgg agcagccgct ccagctcctc ggccgagacc
     8461 gtccggccca gcgtgagtcc ccagcggtcc tgctcgagcg agcgcacggc cttgtgcagg
     8521 gcctccttga acgtgcggcc gatggccatg acctcgccca cggatttcat ctgagtggtc
     8581 agggtgggat cggcctcggg gaacttctcg aaggcccagc gggggatctt gacgacgcag
     8641 tagtcgagga ccggctcgaa cgaggccggg gtctcccgcg tgatgtcgtt gcgcagctcg
     8701 tcgagggtac agccgacggc gagcttcgcg gcgatcttgg cgatggggaa gccggtggcc
     8761 ttggaggcca gcgcggagga gcggctcacg cgcgggttca tctcgatcac gaccagccgc
     8821 ccgtcgcgcg ggttgacggc gaactggatg ttggagccgc cggtctcgac gccgatggcg
     8881 cggatgatgg ccgcggcggc gtcgcgcatg agctgatatt ccttgtcggt cagggtctgg
     8941 gcgggggcga cggtgatgga gtcgccggtg tggacgccca tgggatcgag gttctcgatc
     9001 gagcagatga cgacgacgtt gtcggcgagg tcgcgcatga cctcgagctc gaactccttc
     9061 cagccgagca cggactcctc gaccagcacc tggccgaccg gcgactgggc cagggcccag
     9121 cggacggcgg gttcgagatc ggcggccgta tgagcgatgg cgccgcccga gccgccgagg
     9181 gtgaaggacg gacggagcac gagagggaag cccagctcgg tggcggcccg tcgcgcctcg
     9241 tcgagcgagg tcacctggtg ggagcgcggc agggcgagcc cgagcgcctc gatggtctgc
     9301 ttgaagaggg cgcggtcctc ggccttccgg atggcgggga gcttggcgcc gatcagctcg
     9361 acgccgaggc ggtcgagggt gccgtcctcg gccagcgcca cggccagatt cagcccagtc
     9421 tgcccgccca gagtcggcaa gagcgcgtcg ggacgctcgc gggcgatgac cttgccgacg
     9481 aactccgggg tgaggggctc gacgtaggtg cgggtggcga gctcgggatc ggtcatgata
     9541 gtggcggggt tggagttgac gaggacgacc tcgaacccct cctcgcggag cgcgcggcag
     9601 ccctgggtgc cggagtagtc gaactcgcag gcctggccga tgacgatggg ccccgagccg
     9661 atgatgagga ccttccggat atccgtccgc ttcggcactg cccgctactc ttccaccagc
     9721 ccgtagacga tcgaggtttc cggcgtcgct tcctcgcggc ggtagaggac gtcgtcgagg
     9781 cggccgccgc cgatccacac ggcctcgccg ttgccccaga gctgctcgcc gagcacgctc
     9841 atgcgcttgg ccacgcggtc gcgcaccgca tgcgcctcga gcgacgcatc gctctgcgtg
     9901 gtgaacgacg cccactccgg cttgtgaccg ggaccttccg tcttgcggta ggagatcaag
     9961 tacagaggca tggggcggct ccccctccct tccggaccat caggtccgtg aaccgaatgg
    10021 cgttgtcgag gggcgccccg gctgcgccgg ggcgcccgga gcgcccggga ggcttggggg
    10081 acgcccggag ccccccatcc ccatgaggtc cgtgaaccgt tcgaacagat agctcgcgtc
    10141 gtgcggtccc ggcgacgctt ccgggtggta ctgcacggag aacgcgggcc actcccgatg
    10201 gcgcaagccc tcgcacgtcc cgtcgttgag gttgacgtgc gtgggctccc agcccgcgcg
    10261 ctcgacggat gccgggtcga cggcgaaccc gtggttctgc gaggtgatct ccacgcggcc
    10321 cgtcggcaga tccttgacgg ggtgattcgc cccgtgatgc ccgaagggca gcttgtaggt
    10381 gcggccgccc gcagccaggc cgaggatctg gtggccgaga cagatgccga agacgggcac
    10441 gcgcccgagc aggccgcgca cggcgtcgat cagatagggc acgccttcgg gatcgccggg
    10501 gccgttggag aggacgacgc cgtccggcct tcgctcgagc acggccgcga cgggcgtcgc
    10561 cgcgggcacc accgtcacgg cgcaccccga ggccgcgagc tggcgcagga tgttgagctt
    10621 gatgcccgcg tcgtaggcga cgaccccgaa gcgcggcggc gccggcggct ggtagccggc
    10681 gccgagacgc cacgtgccct cgtcgtgcca cgtgtaaggc gcgacgcagg tgacctcgcc
    10741 gacgagatcg cgtccgacga gcccgggcag cgcgcgggcc cgctcgcgca agcggtccac
    10801 gtcgaggtcg gtggaggaga cgatgccgtc ctgcgcgccg tgatcgcgca ggtggcgggt
    10861 caacgcgcgc gtgtcgatcc cctgcaggcc gacgatgccg tgggtcctca gatactcgtc
    10921 gagggggacc tgggcccgcc aggacgaggg gcgcagcgcc ccttcccgga cgatgaaggc
    10981 gttgacccag gggcggcggg actcgacgtc ctgggtgttg acgccgtagt tgccgatcag
    11041 gggataggtc atggcgacga tctggccgcg ataagaggga tcggtgagga tctcctggta
    11101 gccggacatg gcggtattga agatgacctc tccaccggcc tcgccgatgg cgccgagggc
    11161 ctcgcctcgg aacacgcgcc catcgcgcag gaccaggagc gcgtccgtca tgaggagaac
    11221 accttcctgc cgcccacgat cgtcatccac ggcccgccgc ggagcgtcca gccggcgaac
    11281 ggcgtgttcc ggctacggga gtgaaaccgc gcgggatcca ccgtgagttc ccggtcgagg
    11341 tcgaggatcg tgac
//
LOCUS       DATIAZ010000097         8454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1864934, whole genome shotgun sequence.
ACCESSION   DATIAZ010000097 DATIAZ010000000
VERSION     DATIAZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864934"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1012)
                     /locus_tag="VLF19_03350"
     CDS             complement(215..1012)
                     /locus_tag="VLF19_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE92316.1"
                     /translation="MTLNALEWGAPGGPGLCLLHGGAAHAHWFDLVAPAFAERFHVIA
                     LDQRGHGESDWPRPAAYGTEDFAADLLGVFDALGWDRVILAGHSMGGHNAMAFASWHP
                     ARVRALVVIDSRPALPEDRLSMMHRRGERPVRRYATAGEAVAAFRLLPRDTVADPVLL
                     AHLARAGVAQRDGGWSLRFDPDANRTRRPVDNWALVSRITAPTLIVRGERSPILPPDM
                     AERLRRAITDARLVEIAGAYHHLVLDRPAEFTAVLARFLIDCIEGHD"
     gene            1161..2783
                     /locus_tag="VLF19_03355"
     CDS             1161..2783
                     /locus_tag="VLF19_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HSE92317.1"
                     /translation="MKVEGTPKGSTARHLVDGGYALGLLASAVLFGYVILSPSTPHAR
                     TVPTRVAAVVSTDRTVGLGQPPVLTLESYPDLPVTRSRESALPSVARTLPPERMLARS
                     RPAPSEHRRGRPAPLEPLAPVEWPPSPAGEGVTWPEKVTGRVLDQDGNPLAGASVVLA
                     GRELRADAEGAFTLTPAAGSAPMIVKLPGYDRLVVAPRREPVEAVLRPRAAKAAYLTY
                     FGIADRGIRGRVLDLLTRTELNAVVIDVKGDRGWIPYRTEVPSAIAAGAQGPVIIRDF
                     EGMIADLRARGVYTIARVVTFKDNVLATARPDLAIVDTRTGKPWVDNEKLAWVDPFRE
                     EVWAYNIEVAREAARKGFDEVQFDYVRFPTDGKLGAARYAKPNSKQTRLPAIAAFLER
                     ARRELGPMGVYVAADVFGYTAFNDNDTDIGQRVEEMAPHLDYICPMVYPSGYHVGIPG
                     YRNPVANPYAVVHESVRLIRKRAEGTQVRVRPWLQDFKDYAFDRRVFGVTEIRAQIKG
                     SDDAGGAGWMLWNPRNAYTGAALLPESPLAAR"
     gene            2780..3745
                     /locus_tag="VLF19_03360"
     CDS             2780..3745
                     /locus_tag="VLF19_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSE92318.1"
                     /translation="MRRLGPLALALVALLAAFPVAPVAAQPLAPNELGRVMILEYHKI
                     DYPEERWTRTPENFRRDLETLYARGYRLAALNDLLDGVVRTPAGTTPVVLTFDDSSPG
                     QFRYVGHQSAPEIDPKSGVGILEAFVRERPDFGRAATFFVLPGASRPNRLFDQPEHEG
                     RKLRYLVERGYEIGNHTLWHANLGKYDEATVRGQLADAQIWVQRHVPNYMFRTLALPH
                     GVYPRDVAWALTGTAKGTTYRHDAILMVAGGAAPSPFSRGFDAARLPRIQAVERDLTH
                     WLSYFDKNPQERFVSDGDPESVAVPIRLRDKLRSPVPRNLRIVQR"
     gene            complement(3821..6214)
                     /locus_tag="VLF19_03365"
     CDS             complement(3821..6214)
                     /locus_tag="VLF19_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HSE92319.1"
                     /translation="MLRWSVALAHGLRILLLAALAVLLAVAIAPAQTPDVRVPDFTGV
                     DDAARDAVVSGEIPGAVIFIGRGDEVLYHRAWGSRTLIPGPTPMATDTIFDIASLTKP
                     LGTTLAVMALAERGAVRLDAPLGRYLREFNGHAFEHVTVRRVLSHTAGFPALPAHGTL
                     TAGFPAAGRALASRALDYAPGTGFQYSDTGFMLLGELVRRVSGQPLDRYLERIVFRPL
                     GLRDTSFHPPDAVRARVAPTEFANGILLQGRVHDPRARLLDGVAGHAGMFSTARDLSR
                     ICRMLLDGGALGDKRILKAVTVRMMWSRSPDTEMRVLGWDVSSPYAQPMAPFFPAASL
                     GHTGFTGTAVWLDPESRGFMILLTNRVHPSGGGAVKIRELRARVAAAAGAALFAPEPP
                     AASTVSSSPAADGAESTGGVSPIAGPPSAPVVRTGLDVVAQQGFASLHGHAIGLVTNQ
                     TGVDALGRRNIDLLAAAPGVRLVAVFSPEHGITGTENTEVAHGRDTATGVPIWSLYGP
                     ARRPTSEMLRGISLLVFDIQDVGARYYTYLTTLVYVMEEAGRRGIPVVVLDRPNPING
                     RVVEGPVMDPDLKSFTAPHPIPVRTGLTIGEFGRLVAGERKLPVSLTVVPVAGWDRGR
                     WFDDTGLPWVNPSPNIRSVTQALLYSGIGLLEATNLSVGRGTDSPFEVIGAPWIDAPR
                     LAEALNRLRMPGVRFDPGTFTPAADRYANTPCWGVRMVVTDREATRPVTVALALGRAL
                     REHHREQFRAENIQHLLVHRPTMWAFLRSEPLDRLVAWADAERASFLNRRASYLIYP"
     gene            6246..7142
                     /locus_tag="VLF19_03370"
     CDS             6246..7142
                     /locus_tag="VLF19_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HSE92320.1"
                     /translation="MQHVFVTGATGFVGRAIVHALCAEGHVVRCLVRRGSEGDLHGLG
                     GIERVEGDVLVRRGLEDGIAGCQAVIHLVGIIREAPARGVTFENVHAQGTRNVLDAAI
                     SAGVRRFVHMSALGTRAGARARYHQTKWAAEDAVRGSGLAWTIFRPSIIYGRGDGFVT
                     RLARMVRRLPVVPVLGTGRNLLQPVPVEHVGEAVARALTRPVSEKQTYDVAGPDRVTM
                     LELLDLIGAALGRRRVRKVHVPLSLVRPLARVLHPLPGFPVTPDQLLMLEENNVGDPT
                     AFYQHFGIEPLPLRRGIERTLT"
     gene            7149..7589
                     /locus_tag="VLF19_03375"
     CDS             7149..7589
                     /locus_tag="VLF19_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92321.1"
                     /translation="MPFRTRPDQPRDLLSLIESEVRERIEEAVDHVSLEAIVAARRAR
                     GLPAPAADNPRDRAEFEAGVGAFLERLRADLEPALTDEIRRKADEAAARAGHDALARL
                     VGAQVALARALPDYWQRFDLSRTAYAAAASGGKRRGLFDRLLAR"
     gene            complement(7542..7862)
                     /locus_tag="VLF19_03380"
     CDS             complement(7542..7862)
                     /locus_tag="VLF19_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92322.1"
                     /translation="MEDETMNPVERLLEDEVERLLDRLSGSVPGGCLEATAVRHPSLR
                     RRLDEVEAQMAVVRASLLESYGQWCRSLEDVENLWALAAWKCDGSEASAGEQPVEQAA
                     SLAA"
     gene            7975..>8454
                     /locus_tag="VLF19_03385"
     CDS             7975..>8454
                     /locus_tag="VLF19_03385"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HSE92323.1"
                     /translation="MRWLVLIVGLPREPSSLRVRAWRRLKMAGAVALKRGVWVLPATP
                     ETTEQFQWLAQEVQRDGGDATLLLVERMENVGDGEIVRRFQEARDPDYRALTERYRRL
                     LRGLERQRGEPPQRARDEAARLLREHARLRDVDYFEAPAGAEAQRAREALEARLAPPA
                     "
BASE COUNT         1242 a         3064 c         2982 g         1166 t
ORIGIN      
        1 tgagtcccga actcgccgac cgcaggccgg ggcgggggcg cgggtccacc gacctgttcc
       61 ccgcccacgg gaccccgccc cggccggcgc gatcacctgc gaacctctga ttcacgacac
      121 cagcgagtga aggggcgcta cgctccacgg ccgagcgccc tggggtatcc ggggcccgtg
      181 tcgggccccg atgcgatcag tcgcggagtc gcggtcaatc gtggccttcg atgcagtcga
      241 tcagaaaccg ggcgaggacg gcggtgaact ctgccgggcg atccagcacg aggtggtggt
      301 aggcgccggc gatctccacc agccgagcgt ccgtgatggc cctgcgcagc cgctcggcca
      361 tgtccggcgg caagatcggg ctccgctcgc cgcgcacgat cagggtgggc gcggtgatgc
      421 gtgagaccag cgcccagttg tccaccggcc gccgcgtccg gttggcgtcc gggtcgaagc
      481 gaaggctcca gccgccgtcg cgctgggcca cccctgcgcg cgcgaggtgc gcgagcagca
      541 ccgggtcggc gacggtatcc ctcggcagca ggcgaaacgc cgcgacggcc tcgcccgccg
      601 tcgcgtagcg acgcacgggg cgctccccac ggcggtgcat catcgagagc cggtcctcgg
      661 gcagcgcggg ccgggagtcg atgacgacca gcgcccggac acgcgcgggg tgccacgagg
      721 cgaacgccat cgcgttgtgg ccgcccatcg agtggccggc caggatcacg cggtcccagc
      781 cgagcgcgtc gaacacgccg agcagatcgg cggcgaagtc ctccgtcccg tacgccgccg
      841 gtcgtggcca gtcgctctcg ccgtggccgc gctgatcgag cgcgatcacg tggaaacgtt
      901 cggcgaaggc cggcgccacg aggtcgaacc agtgcgcgtg cgccgcgcca ccatgcagca
      961 ggcaaagccc aggtccaccc ggcgctcccc actcgagagc gttgagtgtc aaaccgccca
     1021 ctctaatccg gcgattcttc gcgactcggc agccgagtgg caattccggc accgtgaaat
     1081 tgtacgcgtg acacgacatg cggaaaattt tttctaggac actgcagacc gcttcagtag
     1141 ttttgaaaac agggattgca atgaaagtcg aaggcactcc gaaaggctca accgcgcggc
     1201 acctcgtcga cggcggctat gcactgggac ttctcgccag cgcagtgctg ttcggctacg
     1261 tgatcctgtc cccgtccacc ccgcatgctc gcacggtccc gacgagggtg gcggcggtgg
     1321 tgtccaccga tcgcaccgtc ggcctcggcc agcccccggt cctgaccctg gagtcgtatc
     1381 cggatcttcc ggtgacgcgg tcacgcgaga gcgcgctgcc ctccgtcgcg cggactcttc
     1441 cgccggagcg catgctggca cgctcgcggc cggctccgag cgagcatcgt cgcggccgac
     1501 cggcgccgct cgagcctctg gcgcccgtgg agtggccgcc ctcgcccgcc ggcgagggcg
     1561 tcacgtggcc cgagaaggtg accgggcgcg tgctcgacca ggacggcaac ccgctggcgg
     1621 gcgcctccgt cgtcctcgcc gggcgcgagc ttcgggccga cgccgagggc gcgttcacgc
     1681 tcacgccggc ggcaggcagc gccccgatga tcgtgaagct gcccggctac gaccggctcg
     1741 tcgtcgcgcc gcgccgagag cccgtcgagg ccgtgctgcg gccccgggcg gcgaaggcgg
     1801 cgtacctgac gtacttcggc atcgccgacc gcggcatccg cgggcgtgta ctcgatctgc
     1861 tgacgcggac cgagctgaac gccgtcgtga tcgacgtcaa gggcgaccgc ggctggatcc
     1921 cctaccgcac cgaggtcccg tccgcgatcg ccgccggcgc gcagggcccg gtgatcatcc
     1981 gcgacttcga ggggatgatc gcggacctcc gggcgcgggg cgtgtacacg atcgcccgtg
     2041 tggtcacgtt caaggacaac gtcctcgcca ccgcgcgccc cgacctggcc atcgtcgaca
     2101 cgcggacggg caagccgtgg gtcgacaacg agaagctcgc ctgggtcgat ccgttccgcg
     2161 aggaagtctg ggcctacaac atcgaggtcg ccagggaagc cgcccgcaag ggcttcgacg
     2221 aggtgcagtt cgactacgtg cgcttcccca ccgacggcaa gctcggtgcc gcgcggtacg
     2281 cgaagccgaa ctcgaagcag acccgcctgc ccgcgatcgc ggccttcctc gagcgggcgc
     2341 ggcgggagct cgggccgatg ggcgtgtacg tcgccgcgga tgtcttcggc tacaccgcgt
     2401 tcaacgacaa cgacaccgac atcggtcagc gggtcgaaga gatggcgccg cacctcgact
     2461 acatctgccc gatggtctat ccgtcgggct accacgtcgg gatccccggt tatcgcaacc
     2521 cggtggcgaa cccctacgcc gtcgtccacg agagcgtccg gctgatccgc aagcgcgccg
     2581 agggcacaca ggtgcgggtg cggccgtggc tgcaggactt caaggactac gcgttcgatc
     2641 ggcgcgtgtt cggcgtcacc gagatccgcg cgcagatcaa gggatccgac gacgccggcg
     2701 gcgcgggctg gatgctgtgg aacccccgca acgcctacac gggtgcggcg ctgctccccg
     2761 agagcccgct cgccgcgcgg tgagacggct cggcccgctc gcgctggccc tcgtcgctct
     2821 cctcgccgcg ttccccgtcg ctccggtcgc tgcccagccg ctcgccccca acgagctcgg
     2881 gcgggtcatg atcctcgagt atcacaagat cgactacccc gaggagcgct ggacgcgcac
     2941 gccggagaac ttccggcgcg atctcgagac gctctacgcc cggggctacc ggctggccgc
     3001 cctcaatgac ctgctcgacg gcgtcgtgcg gaccccggcc ggcaccacgc cggtggtgct
     3061 cacgttcgac gactcctcgc ccggccagtt ccgctacgtg gggcaccaga gcgccccgga
     3121 gatcgacccg aagtcgggcg tggggatcct cgaagcgttc gtccgcgagc ggcccgactt
     3181 cgggcgtgcg gcgacgttct tcgtgctgcc cggcgccagc cgccccaacc ggctcttcga
     3241 tcagcccgag cacgagggcc ggaagctgcg ctacctcgtc gagcgtggct acgagatcgg
     3301 caaccatacg ctctggcacg ccaacctggg caagtacgac gaggccaccg tccgcggcca
     3361 gctcgccgac gcccagatct gggtgcagcg tcacgtgccg aattacatgt tccgcacgct
     3421 ggcgctgccg cacggcgtgt acccgcgcga cgtcgcgtgg gcgctgacgg gcaccgccaa
     3481 gggcacgacg taccggcacg acgccatcct gatggtcgcc ggcggcgcgg cgccctcgcc
     3541 gttctcgcga ggcttcgacg ccgcgcgcct gccgcgcatc caggcagtgg agcgtgacct
     3601 cacccactgg ctctcgtact tcgacaagaa tccgcaggag cgcttcgtga gcgacggcga
     3661 tcccgagagc gtcgccgtcc ccatccggct ccgcgacaag cttcgcagcc cggtccctcg
     3721 caacctgcgc atcgtccaac gctgacccgc cgcggccgtc gccgcgagac acgagtcccg
     3781 cttcacctca gtgccggcgg cacggcaagc aagcgcggag ctaggggtag atgagatacg
     3841 acgcccgccg gttcaggaag ctcgcgcgct ccgcgtccgc ccacgcgacg agccggtcga
     3901 gcggctcgct cctgaggaag gcccacatcg tgggacggtg gaccagcagg tgctggatgt
     3961 tctcggccct gaactgctcg cggtgatgct cgcgtagcgc gcgcccgagc gcaagcgcca
     4021 ccgtcaccgg gcgggtcgcc tcgcggtcgg tgacgaccat ccgcacgccc cagcagggcg
     4081 tgttcgcgta tcggtcggcg gcgggggtga acgtgccggg atcgaatcgc acgccgggca
     4141 tgcgcagccg gttgagcgcc tccgccagcc ggggggcgtc gatccacggc gcgccgatca
     4201 cctcgaaggg cgagtcggtt ccacgcccga cggacaggtt ggtcgcctcc agcagaccga
     4261 tgcccgaata cagcagcgcc tgcgtcaccg agcggatgtt cggcgaggga ttcacccatg
     4321 gcaggccggt gtcgtcgaac catcgcccgc ggtcccaccc ggcgaccggc acgaccgtga
     4381 gcgacaccgg cagcttccgc tcgccggcga ccagccgccc gaactcgccg atcgtcagcc
     4441 cggtgcgcac ggggatgggg tgcggcgccg tgaaggactt gagatccgga tccatcaccg
     4501 gcccctcgac cacgcgcccg ttgatcgggt tcgggcggtc gagcacgacc accgggatgc
     4561 cgcgccgccc ggcctcctcc atcacgtaca cgagcgtcgt gaggtaggtg tagtaccggg
     4621 cgccgacgtc ctggatgtcg aagacgagca ggctgatgcc gcgcagcatc tcggacgtcg
     4681 ggcgtcgcgc gggcccgtag aggctccaga tcggcacccc ggtggccgtg tcgcgcccgt
     4741 gcgcgacctc cgtgttctcc gtgcccgtga tgccgtgctc cggcgagaag acggcgacga
     4801 gccgcacgcc gggcgcggcg gccaggaggt cgatgttgcg acggccgagg gcgtcgaccc
     4861 cggtctggtt ggtgaccaga ccgatcgcgt ggccgtggag agacgcgaac ccctgctggg
     4921 cgacgacgtc gagcccggtc cgcacgacgg gcgcgctcgg cggaccggca atcggggaga
     4981 cgccaccggt gctctccgcg ccgtcggccg cgggcgacga cgagaccgtc gaggccgcgg
     5041 gcggctccgg cgcgaagagc gccgcgccag cggcggcggc cacccgcgcc cgcagctcgc
     5101 ggatcttcac ggcgccgccg ccgctcgggt gcacgcggtt ggtgagcaag atcatgaagc
     5161 cgcggctctc gggatcgagc cagaccgccg tgccggtgaa gcccgtgtgg cccagcgagg
     5221 cggccgggaa gaacggcgcc atcggctgcg cgtacggcga ggagacgtcc cagcccagca
     5281 cccgcatctc cgtgtccggc gaccggctcc acatcatccg caccgtgacc gccttgagga
     5341 tcctcttgtc accgagcgcg ccgccgtcca gcagcatgcg gcagatccgc gacaggtccc
     5401 gggccgtcga gaacatgccg gcgtgccccg ccacgccgtc cagcaggcgc gcgcgggggt
     5461 cgtgcacgcg tccctgcagg aggatgccgt tggcgaactc cgtcggggcg acgcgcgcgc
     5521 ggacggcgtc ggggggatgg aagctcgtgt cccgcagccc gagcgggcgg aacacgatgc
     5581 gctcgagata ccgatcgagc ggctggccgc tgacccggcg tacgagctcg ccgaggagca
     5641 tgaacccggt gtcgctgtac tggaagcccg taccgggcgc atagtccagc gcgcgactcg
     5701 cgagcgcccg gcccgccgcc ggaaagcccg cggtgagcgt gccgtgcgcg ggcagcgcgg
     5761 ggaagccggc ggtgtggctg agcacgcggc ggacggtgac gtgctcgaag gcatgcccgt
     5821 tgaactctct caggtagcgt ccgagtggcg cgtcgagccg gacggcgccg cgctcggcca
     5881 gcgccatgac ggccagcgtc gtaccgaggg gcttcgtcag cgaggcgatg tcgaagatgg
     5941 tgtccgtggc catcggtgtc ggcccgggga tcagcgtgcg cgagccccag gcccggtggt
     6001 acagcacctc gtcgccccgt ccgatgaaga tgacggcccc cgggatctcg ccggagacga
     6061 cggcgtcccg ggcggcgtcg tccaccccgg tgaagtccgg gacccgcacg tcgggtgtct
     6121 gggccggggc gattgcgacg gcgaggagga cggcgagcgc agcaaggagt aggatacgga
     6181 ggccgtgagc gagggccacg gaccatctta acattgatat ccttgagtta aggcaggacg
     6241 cagcgatgca gcacgtcttt gtcacggggg ccacggggtt cgttggacgg gccatcgtgc
     6301 acgcgctgtg cgccgaaggt cacgtcgtcc ggtgcctcgt gcggcggggc tccgagggcg
     6361 acctgcacgg cctcggtggc atcgagcgcg tggagggcga tgtcctcgtc cggcgcgggc
     6421 tcgaagacgg catcgcgggc tgccaggcag tcattcatct cgtcgggatc atccgcgaag
     6481 cgcccgcccg cggcgtgacg ttcgagaacg tccacgccca gggcacccgc aatgtacttg
     6541 acgcggcgat cagcgccggg gtgcgccgct tcgtgcacat gagcgcgctc ggtacccgcg
     6601 ccggcgcccg cgcacgctat caccagacca agtgggcggc ggaagacgcc gtccggggca
     6661 gcgggctcgc gtggaccatc ttccgcccgt cgatcatcta cggtcggggc gacggcttcg
     6721 tgacgaggct ggcgcggatg gtccgccgcc tcccggtcgt gcccgtgctc ggcacgggac
     6781 gcaacctgct gcagcccgtc cccgtcgagc acgtcggcga ggccgtcgcg cgggcgctga
     6841 cgcggccggt gagcgagaag cagacctacg acgtggccgg ccccgaccgc gtgacgatgc
     6901 tggagctcct cgatctcatc ggcgccgcgc tcgggcggcg gcgcgtgcgc aaggtgcacg
     6961 tcccgctgtc gctcgtgcgt cccttggcgc gcgtgctcca cccgttgccc ggcttcccgg
     7021 ttacgcccga tcagctcctg atgttggagg agaataacgt cggcgacccc accgccttct
     7081 accagcactt cggcatcgag ccgctgccgc tgcggcgcgg aatcgaacgc acgctgacct
     7141 gagcccccgt gccgttccgc acccgccccg accagccacg cgacctcctg tcgctcatcg
     7201 agagcgaagt tcgcgagcgc atcgaggagg ccgtcgacca cgtcagcctc gaggcgatcg
     7261 tcgccgcgag gcgcgcgcgg gggctccccg ccccggccgc cgacaacccg cgcgaccggg
     7321 ccgagttcga agccggcgtg ggcgctttcc tggagcgcct gcgcgccgac ctcgagccgg
     7381 cgctcaccga cgagatccgg cgcaaggccg atgaggccgc ggcgcgcgcc ggacacgatg
     7441 ccctcgcgcg cctcgtcggg gcgcaggtcg ccctcgcccg cgcgctgccg gactactggc
     7501 agcggttcga tctctcgcgc accgcgtacg cggcggcggc ctcaggcggc aagcgacgcg
     7561 gcctgttcga ccggctgctc gcccgctgac gcctcggacc cgtcgcactt ccacgccgcc
     7621 agcgcccaca ggttctcgac gtcctccagg cttcggcacc actggccgta actctcgagc
     7681 agcgacgccc gcaccaccgc catctgcgcc tcgacctcgt cgagccggcg ccgcagcgac
     7741 gggtggcgta ccgcggtcgc ctccagacag cctccaggca ccgaccccga cagccggtcc
     7801 aggagccgct cgacctcgtc ctccagtagt cgttccaccg ggttcatcgt ctcgtcctcc
     7861 aacaatgtct tatcggggct catatactcg tcacatgatt agtctattgt caatcccccc
     7921 cccagttgat ctgatgaaac acatgatgta gccttgcaac ctgtgattca acaaatgcgc
     7981 tggctcgtac tcatcgtcgg acttccacgc gagccgtcca gcctgcgcgt gcgagcgtgg
     8041 cggcggctca agatggccgg cgctgtcgcg ctcaagcgcg gtgtctgggt gctgccggcc
     8101 acgcccgaga cgaccgagca gttccagtgg ctcgcccagg aagtccagcg cgacggtggc
     8161 gacgcgacgc tgctgctcgt ggagcggatg gagaacgtcg gtgacggcga gatcgtgcgg
     8221 cgtttccagg aggcgcgcga ccccgactat cgcgcgctga ccgagcggta ccgtcggctg
     8281 ttgcgtgggc tggagcgcca gcggggcgag ccgccgcagc gcgcgcgcga cgaggccgcg
     8341 cggctcctgc gggagcacgc gcggctgcgg gacgtcgatt acttcgaggc gccggcggga
     8401 gctgaggcgc agcgcgcgcg cgaggccctg gaggcgcggc tggcgccgcc ggca
//
LOCUS       DATIAZ010000098         5556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1208157, whole genome shotgun sequence.
ACCESSION   DATIAZ010000098 DATIAZ010000000
VERSION     DATIAZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208157"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="VLF19_03390"
     CDS             complement(<1..1894)
                     /locus_tag="VLF19_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSE92324.1"
                     /translation="MSYSLGIDIGGTFTDIVVYDERCGRQLNCKVLTTHDNPARAVAA
                     GVEALLRRHRLPAGEFTRVVHATTLFTNALIERRGAVTGLVTTAGFADTLEIGRERKF
                     ELYDLNIVKPEPLVPRHLRLEVAERTRADGSIARRLDARQVAARARQLADAGATSIAV
                     VFLHAYANPRHEAEAARVIARRHPGIVVTASHEVAPEIREYERASTTVANAYIKPLAR
                     LYLDTMARRVAGLGIPAPLLLMLSSGGLTHVAEAERTPVQMLESGPAAGALAAAFFGR
                     EDSRGNLLAFDMGGTTAKLSLVDGGEPLTAYAFEAARQKRFLEGSGLPIRISTIELIE
                     IGAGGGSIAHVDEIGLLKVGPRSAGSQPGPAAYGLGGAEPTVTDADFVLGYLNPGYFA
                     GGEVRVDIAAARAALEQLTGRVGLSLTEVAWGIHDVVNENMAGAARVHIAERGRDPRD
                     YALLCTGGAGPVHAYEVARKLGLREVICPPAAGVASALGLLVAPARVDRVATVGIRLD
                     RDSVGALEKAFRRLEDDARAVMADTGLELDTASVRRLADGRFLGQGFDLVVELPDGPY
                     DDSPDSRQRLTGAFETAYREKFALTPPDVPIEFINIRVAVRAPVAGELRGGGAPSDRG
                     RADVDVA"
     gene            2144..3019
                     /gene="murB"
                     /locus_tag="VLF19_03395"
     CDS             2144..3019
                     /gene="murB"
                     /locus_tag="VLF19_03395"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSE92325.1"
                     /translation="MSFHTSLRIGGPAEFLVVPHDIDDARHALLFAAQEGLPVIVVGG
                     GNNLLVSDCGVQAVVLKLQGILSRTQFDGEEAVVGAGVPLSELIRDASAQGLGGLEEF
                     AGIPASIGGALAMHVESGGRAFENLCTAVYFLYRDGTLGEIRPSGGHVFRNLHPASTV
                     LLGCRLRLTRRSQADIRRDIQQRLKQKRTTQPFALASAGYIWKRAGSEPAERLIERAG
                     LRGKRVNSAEISTKCANFVVNRGGATAADVRALMEMMAETVERRFGVTLRPEIRMLGF
                     ERAGVETSPRELVAS"
     gene            complement(3118..3528)
                     /locus_tag="VLF19_03400"
     CDS             complement(3118..3528)
                     /locus_tag="VLF19_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92326.1"
                     /translation="MSDRRYRQQGYRQSDAAKREPPRRPPSADTPRSGPMLGQRALSR
                     CAACGAVLPIATSALEQCPSCRAAIHACRQCTHFEPGRRFECARPIPERITDKSTRNE
                     CAEFSLRVTVERDASPDGPRPGDARRAFGDLFKK"
     gene            complement(3562..4038)
                     /locus_tag="VLF19_03405"
     CDS             complement(3562..4038)
                     /locus_tag="VLF19_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92327.1"
                     /translation="MALQVRRVITGHDANGRAIVKIDEVSKNLSSGRPGATACNIWTT
                     EGFPVDNDGAADEGLRKVATTLKNGTIFRVIEFAPGLAARNHRTDSIDYIVVVSGEID
                     MELDDSRVHLKAGDVMVQRGTIHNWVNRGTAPCVLAVVLVDAKPVEAGGKVLHAIG"
     gene            complement(4038..5192)
                     /locus_tag="VLF19_03410"
     CDS             complement(4038..5192)
                     /locus_tag="VLF19_03410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92328.1"
                     /translation="MRLSLAYEMQRPVVDDHAVIEETLQQCLLADEMGFDAVWFVEHH
                     FLTSFSMSPCPEVIFGALSRLTKRIRLGFGVVILPYHHPVRVAERVAMVDHMSGGRVE
                     FGTGRSAPYEQIGMGIDPRETRARWEESLRMIPRIWEDGLFSWEGTFWSVPARDVRPK
                     PYQKPHPRIWVAALQPATYQLAAELGIGVMALSVAAPSHLAPHIKAYKERVRGAEPVG
                     KFINDQWLSSTMAVCDWDSKGARELAARSLRTFFGPDRPYLKDQTHLYEQLVESWGGV
                     PEHLKANFARYLKTEGAAGAPEVDLSGGSGQIASAMWNQIDADTLVDRGVLIAGDPES
                     CLRGIAIHEEAGVDELQFLMATETVSHDKVMSSIEMFGRHVIPKLKGGGR"
     gene            complement(5295..>5556)
                     /locus_tag="VLF19_03415"
     CDS             complement(5295..>5556)
                     /locus_tag="VLF19_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HSE92329.1"
                     /translation="QSSQRPIAVHALEHLASTDRGVIMLRRIVREGIRAVARGGDPPG
                     ADRPEGKVIRTFTQDVVLRVPPAPTPEADRRLLRATGRQVVA"
BASE COUNT          792 a         1962 c         1984 g          818 t
ORIGIN      
        1 gggccacgtc cacgtccgcc ctcccccgat cggacggtgc tccacccccc cggagctccc
       61 ccgccaccgg cgcccgcacc gccacccgga tgttgatgaa ctcgatgggg acgtcgggcg
      121 gggtgagcgc gaacttctcg cggtaggccg tctcgaacgc tcccgtcagg cgctgccgcg
      181 agtccggcga gtcgtcgtag gggccgtccg gcagctccac gacgaggtcg aagccctggc
      241 cgaggaagcg gccgtcggcc agccggcgca cgctcgccgt gtccagctcg aggccggtgt
      301 cggccatcac cgcgcgcgcg tcgtcctcga gccggcggaa ggccttctcc agcgcgccga
      361 cgctgtcgcg gtcgagccgg atgccgacgg tggccacgcg gtccacgcgc gcgggcgcga
      421 ccagcaaacc cagcgcggag gccacgccgg ccgccggcgg gcagatgacc tcgcgcagcc
      481 cgagcttgcg ggcgacctcg taggcgtgca cgggcccggc ccccccggtg cagagcagcg
      541 cgtagtcgcg cggatcgcgg ccgcgctcgg cgatgtgcac gcgggcggcg ccggccatgt
      601 tctcgttgac cacatcgtgg atgccccacg cgacctcggt gagcgacagc ccgacgcgcc
      661 cggtcagctg ctcgagcgcg gcgcgggccg ccgcgatgtc cacgcgcacc tcgccgccgg
      721 caaagtagcc ggggttgagg tagccgagca cgaagtcggc gtcggtgacc gtcggctcgg
      781 cgccgccgag gccataggcg gccggcccgg gttgcgagcc ggcgctgcgg gggcccacct
      841 tcagcaggcc gatctcgtcc acgtgggcga tcgagccgcc gccggcgccg atctcgatca
      901 gctcgatggt ggagatgcgg atcggcaggc cgctgccctc caggaagcgc ttctggcgcg
      961 ccgcctcgaa cgcgtacgcg gtgagcggct cgccgccgtc caccaggctg agcttggcag
     1021 tggtgccccc catgtcgaac gccagcaggt tgccgcggct gtcctcgcgc ccgaaaaacg
     1081 ccgccgccag ggcgccggcc gccggtcccg actccagcat ctgcaccggg gtacgctcgg
     1141 cctcggccac gtgcgtcagg ccgccgctcg agagcatgag cagcagcggg gcgggaatgc
     1201 cgagcccggc tacccggcgg gccatcgtgt ccaggtagag gcgagccagc ggcttgatgt
     1261 aggcgttggc cacggtggtg gaggcgcgct cgtactcgcg gatctcgggc gcgacctcgt
     1321 gcgaggccgt caccacgatc cccggatggc gccgggcgat cacccgagcg gcctcggcct
     1381 cgtggcgggg attggcatag gcgtgcagga acacgacggc gatcgacgtc gcgccggcgt
     1441 ccgccagctg ccgggcccgc gcggcgacct gccgggcgtc gaggcgacgc gcgatcgagc
     1501 cgtcggcgcg cgtgcgctcg gccacctcga gacgcaggtg acgcggcacc agcggctcgg
     1561 gcttgacgat gttgaggtcg tacagctcga acttgcgctc gcggccgatc tcgagggtgt
     1621 cggcgaagcc agcggtggtg acgagcccgg tgacggcgcc ccggcgctcg atgagcgcgt
     1681 tggtgaacag cgtggtggcg tggacgaccc gcgtgaactc gccggcgggg agccggtgac
     1741 ggcgcagcaa cgcctcgacg ccggccgcga ccgcgcgggc cgggttgtcg tgggtcgtca
     1801 ggaccttgca gttgagctgg cgcccacacc tctcgtcgta gaccacgatg tcggtgaacg
     1861 tgccgccgat gtcgatgccg agcgagtagc tcacgaccgc ccacgttagg gccgggcgcg
     1921 cgcgaaatca aacgccctcg aaggaggaag tgacgcttct tgtcactcgt ccaggcgttc
     1981 tggcatcccc gagggggacc ccctccttaa gaccttgaaa tccttgaatg cccaaatttg
     2041 gcaccttctg tgcgtatggg gagtgatcac tagtccaagc ctgttcggcg tgaggggcat
     2101 catgctgggg agaatcaggg gcgcggtcag gttcaaggag ccaatgagct ttcacacctc
     2161 gctccggatc ggcggtccgg cggagttcct ggtcgtgccg cacgacatcg acgacgctcg
     2221 ccacgccctg ttgttcgcag cgcaggaggg cctgcccgtc atcgtggtcg gcggcggcaa
     2281 caacctcctc gtatccgatt gcggcgtgca ggcggtggtg ctgaagctgc aagggatcct
     2341 gagccggacg cagttcgacg gggaagaggc ggtggtgggc gcgggcgtgc cgctgtccga
     2401 gctgatccgc gatgcctctg cccagggcct cggcggcctc gaggagttcg ccggcattcc
     2461 cgccagcatc gggggcgcgc tcgcgatgca cgtcgagagc ggtggccgcg ccttcgagaa
     2521 cctgtgcacc gccgtctact tcctctaccg cgacggaacg ctcggagaga tccggccctc
     2581 cggcggccac gtcttccgga acctgcaccc cgcctccacc gtcctcctgg gctgccggct
     2641 gcggctgacg cggcgatcgc aggccgacat ccggcgcgat atccagcagc gcctgaagca
     2701 gaagcggacg acgcagccgt tcgcgctcgc gtcggccggc tacatctgga agcgggcggg
     2761 cagcgagccc gccgagcggc tcatcgagcg cgccggcctg cgcggcaagc gggtgaacag
     2821 cgccgagatc tcgacgaagt gcgccaactt cgtcgtcaac cggggcggcg cgaccgccgc
     2881 cgacgtgcgc gcgctcatgg agatgatggc cgagaccgtc gagcggcgct tcggtgtcac
     2941 gctccgcccc gagatccgga tgctcggctt cgagcgggcg ggagtcgaga cgtcgcctcg
     3001 cgagctcgtc gccagctgag ccgagtcggc gcggcccccg atccgcgggc ctcgcaaggc
     3061 ccaccagacg ctcgctgatc gcccgcgccc accgcggcgg gtcaggtcgg agccaggcta
     3121 cttcttgaag aggtccccga aggcgcggcg cgcgtcgccg ggcctgggcc catcgggcga
     3181 cgcgtcgcgc tcgaccgtga cacgtagcga gaattccgca cactcgttgc gggtgctctt
     3241 gtcggtgatg cgttcgggaa tcggccgggc gcactcgaag cggcggcccg gctcgaagtg
     3301 ggtgcactgg cggcaggcgt ggatcgccgc ccggcagctc ggacactgct cgagcgcgct
     3361 cgtcgcgatg ggcagcacgg ccccgcacgc ggcacaccgc gacagcgcgc gctggccgag
     3421 catcgggccg ctccgcgggg tgtcggcgga cggcgggcgg cgggggggct cgcgcttcgc
     3481 cgcgtcgctc tgccggtacc cctgctggcg atatcgtcgg tcgctcaccc tgaacgtctc
     3541 ctctcgcgat ctggccgcgc cctacccgat ggcgtgcagg accttgccgc cggcctcgac
     3601 cggcttggcg tcgacgagga ccaccgcgag cacgcagggc gcggtgccac gattgaccca
     3661 gttgtggatg gtgccccgct gcaccatcac gtcgccggct ttcaggtgga cgcgcgagtc
     3721 gtccagctcc atgtcgatct cgccggagac gaccacgatg tagtcgatcg agtcggtccg
     3781 gtggttgcgc gccgcgaggc cgggcgcgaa ctcgatgacg cggaagatcg tcccgttctt
     3841 caaggtcgtg gcgaccttcc gcagaccctc gtcggcggcg ccgtcgttgt ccacgggaaa
     3901 accttccgtg gtccagatgt tacaggcggt ggcgccgggc cgccccgagg agaggttctt
     3961 cgagacctcg tcgatcttca cgatcgcccg gccgttcgcg tcgtggcccg tgatcacccg
     4021 ccgcacctgc agtgccatca gcggcctcct cccttcagct tggggatgac gtgcctgccg
     4081 aacatctcga tcgacgacat caccttgtcg tgcgagaccg tctcggtggc catgaggaac
     4141 tgcagctcgt cgacaccggc ctcctcgtgg atggcgatgc cccggagaca gctctccgga
     4201 tcgccggcga tgaggacgcc gcgatcgacc agcgtgtcgg cgtcgatctg gttccacatt
     4261 gccgaggcga tctggccgga gccacccgac aggtccacct cgggcgcgcc ggccgcgcct
     4321 tcggtcttga ggtaccgggc gaagttggcc ttcaggtgct ccggtacgcc gccccaggac
     4381 tccacgagct gctcgtacag gtgcgtctga tccttcaggt acgggcgatc cggcccgaag
     4441 aaggtccgca gcgacctggc ggcgagctcg cgcgcgccct tgctgtccca gtcgcagacg
     4501 gccatggtgg agctcagcca ctggtcgttg atgaacttgc cgacgggctc cgcaccgcgg
     4561 acccgttcct tgtacgcctt gatgtgcggg gcgaggtggg acggcgcggc cacgctgagc
     4621 gccatcacgc cgatcccgag ctcggcggcc agctgatagg tcgccggctg cagcgcggcc
     4681 acccagatgc gcgggtgcgg cttctgatag ggcttggggc gcacgtcgcg cgccgggacg
     4741 ctccagaacg tgccctccca cgagaacagg ccgtcttccc agatgcgcgg aatcatgcgc
     4801 agcgactctt cccatcgggc gcgggtctcg cggggatcga tgcccatgcc gatctgctcg
     4861 tacggcgccg agcggcccgt cccgaactcg acgcgcccgc ccgacatgtg atcgaccatc
     4921 gccacgcgct cggcgacccg tacggggtga tggtagggca ggatgacgac gccgaagccc
     4981 agacggatgc gcttggtcag gcgactcagc gccccgaaga tcacctcggg gcacggcgac
     5041 atcgagaagc tggtgagaaa gtgatgctcg acgaaccaca cggcgtcgaa gcccatctcg
     5101 tcggcgagca ggcactgctg cagcgtctct tcgatgaccg cgtggtcgtc caccaccggc
     5161 cgctgcatct cgtaggcgag gctcaatctc atggatgtgt cccgtggaac agacgcttca
     5221 tcgctcctcc gcgggcagca taccctactc cgacccgatc aggccgccct gacggttcac
     5281 gcgacgaggt gcagtcacgc gacgacctga cggccggtgg cccggaggag ccgccgatcc
     5341 gcctccgggg tgggcgcggg cgggacgcgg agcacgacgt cctgcgtgaa cgtgcggatg
     5401 accttgcctt cgggccggtc ggcgcccggt ggatcgccgc cgcgcgccac cgcgcgaatc
     5461 ccctcgcgca cgatgcgccg gagcatgatc acgccgcgat ccgtcgacgc caggtgctcg
     5521 agcgcgtgca ccgcgatggg gcgctggctc gactgg
//
LOCUS       DATIAZ010000099         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1865199, whole genome shotgun sequence.
ACCESSION   DATIAZ010000099 DATIAZ010000000
VERSION     DATIAZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865199"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VLF19_03420"
     CDS             <1..262
                     /locus_tag="VLF19_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HSE92330.1"
                     /translation="IRQSLDWGKYLDAGYVIAGSPATVRQQLLECIRSLRVGHLMVLL
                     QIGSMPKDLALHNTSLFAREVMPHVRDVWPGYKDRWWPGGAS"
     gene            327..1094
                     /locus_tag="VLF19_03425"
     CDS             327..1094
                     /locus_tag="VLF19_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE92331.1"
                     /translation="MIALRGGAVTFRVLHAGQGTPLVYFHSIHERGGWPPFLERLARG
                     RAVLAPLHPGVGGSTGVETLDDLLDLVLAYEELLDALGVDAADLVGHFFGGMIAAELA
                     ALFPERARRLVLVAPLGLWRDDAPPADVLILPHDELMEVLWADPCSEAAARWAALPEH
                     DAENVAAQVESIARRAAIAKFVWPIPDKGLAKRLHRVQSPTLVLWGGSDRANPVVYAE
                     AWQRRIKGAALQILPGGHMLIHEAPDAAARAVEEFLE"
     gene            1091..1885
                     /locus_tag="VLF19_03430"
     CDS             1091..1885
                     /locus_tag="VLF19_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92332.1"
                     /translation="MSASLSGTAVVTGAGSGIGRATAIMLGARGAAVAAVDLDEAAAR
                     ETAELVAKSGTRAVGLRADVSRAAELDRAVTDAVAALGPLAVMVNNAGILDGYFNVDE
                     TDEALWRRVIDIDLTGVFLGCKRALREMLPRGRGRIVNMASVAGLNGSGGGAAYVAAK
                     HGVVGLTRQMAVVYSSRGITINAVCPGPIVTGLRQHSQAILGPGVPDMSGRGIAVNDE
                     QVRAVVPAGRRGTVEDIASAVCFLVSDEAAYMTGHALVVDGGWRAK"
     gene            1882..3021
                     /locus_tag="VLF19_03435"
     CDS             1882..3021
                     /locus_tag="VLF19_03435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92333.1"
                     /translation="MITKFSVLYVGQVDLDHIGKDGTPADARRYPNERIAEAYFMARE
                     VARTMDELGYHRLWTAEHHFQHEGYEVIPNLILYSTWLATQTKRLKFGCAFNILPMWH
                     PIRLAEDYAMADILTGGRVTMGVGRGYHTREVETFGAPMLDNAANKELFEEQMDVLLK
                     CFNEESWSHHGKHYDLPPAVPYRGYDLRELTCVPRPIHRPVEIWQPIASGKTLDYIAA
                     RGIKGMITLNGERIVDQIFHAYRDAAARAGRRLALGQDLCWGVGFYLADTQEEAIRRL
                     EPAHDERYKWFAPFGFVRYADAEGRPWGTPGAPARVPTLHEGIAQKAWLCGPAAQAIE
                     TIKAFEARYPGLEEVMLHWAEGLPADEFQDQLRRFAREVMPAFRR"
BASE COUNT          489 a         1151 c         1171 g          479 t
ORIGIN      
        1 gatccgccag tcgctcgact ggggcaagta cctcgacgcc ggctacgtca tcgccggcag
       61 cccggcgacg gtgcgccagc agctcctcga gtgcatccgg tcgcttcgcg tcggccatct
      121 gatggtgctg ctccagatcg ggtcgatgcc gaaggacctc gcgcttcaca atacgtcgtt
      181 gttcgcgcgc gaggtgatgc cgcacgtgcg cgacgtgtgg ccgggctaca aggaccgctg
      241 gtggcctggc ggtgcgagct gagcgacggc cgcgagcgcc tcgcgagcgc gccgggaggc
      301 gaaacggtga aatgatcgaa gcacgcgtga tcgccctgcg aggcggcgcc gtgaccttcc
      361 gcgtgctcca cgcggggcag gggacgccgc tcgtctactt ccactcgatc cacgagcgag
      421 gcggctggcc gccgttcctc gagcgcctgg cccgcgggcg ggccgtcctg gcgccgctcc
      481 acccgggcgt gggcggctcc accggcgtcg agaccctcga tgacctgctc gatctggtac
      541 tggcctacga ggagctgctc gatgccctgg gcgtggacgc cgcggacctc gtcggccact
      601 tctttggcgg catgatcgcg gccgagctgg cggcgctctt tcccgagcgc gcgcgccggc
      661 tcgtcctcgt cgccccgctc ggcctgtggc gcgacgacgc cccgccggcg gacgtgctga
      721 tcctgcccca cgacgagctg atggaggtgc tctgggcgga cccgtgctcc gaggccgcgg
      781 cgcgctgggc agcgctgccg gagcacgacg ccgagaacgt cgccgcccag gtcgagtcca
      841 tcgcacggcg cgccgccatc gcgaagttcg tgtggccgat ccccgacaag ggcctcgcca
      901 agcgcctgca tcgcgtgcag tcgccgacgc tcgtgctctg gggcgggagc gaccgcgcca
      961 accccgtcgt ctatgccgag gcctggcagc gccgcatcaa gggcgcggcc ctgcagatct
     1021 tgcccggagg gcacatgctg atccacgaag cgcccgacgc cgcggcgcgg gcggtggagg
     1081 agttcctgga atgagcgcat ctctgagcgg aacggccgtc gtcaccggcg cgggctccgg
     1141 catcggtcgc gccaccgcca tcatgctggg ggcgcgcggc gccgcggtgg cggcggtgga
     1201 cctcgacgaa gcggcagccc gggagaccgc cgagctcgtg gccaagagcg gcacccgggc
     1261 cgtgggcctg cgagccgacg tgagccgggc ggccgagctg gaccgcgcgg tgaccgacgc
     1321 cgtcgccgcc ctgggcccgc tcgcggtgat ggtcaacaac gccgggatcc tcgacggcta
     1381 cttcaacgtc gatgagactg acgaggccct gtggcggcgc gtcatcgaca tcgacctcac
     1441 cggcgtcttc ctcggctgca agcgcgcgct gcgcgagatg ctgccgcggg gccgcgggag
     1501 gatcgtcaac atggcctcgg tggcgggcct caacgggagc ggcggcggcg ccgcctacgt
     1561 ggccgccaag cacggcgtgg tcgggctcac gcgccagatg gcggtggtgt actcgtcgcg
     1621 cgggatcacc atcaacgcgg tctgtccggg accgatcgtg accggtctcc gtcagcattc
     1681 ccaggcgatt ctcggccccg gtgtgccgga catgagcggt cgcggaatcg ccgtcaacga
     1741 cgagcaggtc cgggcggtcg tgcccgccgg acggcgcggc accgtggagg acatcgcgtc
     1801 cgcggtgtgc ttcctcgtct ccgacgaggc cgcgtacatg accggccacg cgctcgtggt
     1861 cgatggaggc tggagggcca agtgatcacc aagttctccg tgctctacgt ggggcaggtc
     1921 gacctcgacc acatcggcaa ggatggtacg cccgcggacg cgcggcgcta tccgaacgag
     1981 cgcatcgccg aggcctactt catggcgcgc gaggtcgcgc ggacgatgga cgagctcggc
     2041 taccaccgtc tgtggacggc cgagcaccac ttccagcacg agggctacga ggtcatcccg
     2101 aacctgatcc tctattcgac gtggctggcc acgcagacga agcggctcaa gttcgggtgc
     2161 gccttcaaca tcctgcccat gtggcacccg atccgcctgg ccgaggacta cgcgatggcc
     2221 gacatcctca ccgggggccg cgtgaccatg ggcgtgggcc gcggctatca cacgcgcgag
     2281 gtggagacct tcggcgcgcc gatgctcgac aacgccgcca acaaggagct gttcgaggag
     2341 cagatggacg tgctcctcaa gtgcttcaac gaggagtcgt ggagccacca cggcaagcac
     2401 tacgatctgc cgccggcggt gccctatcgc ggctacgacc tgcgcgagct gacgtgcgtg
     2461 ccccgtccga tccaccggcc cgtggagatc tggcagccca tcgccagcgg caagacgctg
     2521 gactacatcg ccgcgcgagg gatcaagggc atgatcacgc tcaacggcga gcgcatcgtc
     2581 gaccagatct ttcacgccta ccgcgacgcg gcggcgcgcg ccggacgccg gctcgccctc
     2641 ggccaggacc tctgctgggg cgtgggcttc tacctcgccg acacgcagga ggaagccatc
     2701 cgccgcctcg agcccgcgca cgacgagcgc tacaagtggt tcgcgccgtt cggcttcgtc
     2761 cgctacgccg acgccgaggg gcggccgtgg ggcacgcccg gcgcgccggc gcgcgtgccc
     2821 acgctgcacg agggcatcgc gcagaaggca tggctctgcg ggcccgccgc gcaggcgatc
     2881 gagacgatca aggcgttcga ggcgcgctat ccgggcctgg aggaggtcat gctccactgg
     2941 gccgagggcc tgcccgcgga cgagttccag gaccagctcc ggcgcttcgc gcgcgaggtg
     3001 atgcccgcct tccggcgctg acgaccccat acccctcccc ggtatttttc agagggtgga
     3061 tcgcccccaa gggatggtgt tttatctttt cgccccctcg tacatgtggt agtaataccc
     3121 ccgggaatcc agcgcgccgg gggccctcga ccacggcgag gaggaggaca gatggcgaag
     3181 gcgaagaagg cgttcggcgg gtactcgatc agcttcaagg ggtgcaagga cagcctggag
     3241 tcgctcttcg gcagcgcgcc gatcggaccc tccgtgatga ccaagaagct
//
LOCUS       DATIAZ010000100        13030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1076996, whole genome shotgun sequence.
ACCESSION   DATIAZ010000100 DATIAZ010000000
VERSION     DATIAZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13030
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076996"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VLF19_03440"
     CDS             <1..390
                     /locus_tag="VLF19_03440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE92334.1"
                     /translation="EADRVRRYCDGVGLAPDVTFVVESSDIALTIAGRVPDALDVVFI
                     DGAHGFPAPILDWHYTARRLRIGGLLGVDDYKMPSVRILYDFLRHEEEWTLEKMSWNT
                     AFFRKLREPTELVDWSGQRINAGFRGW"
     gene            440..1555
                     /locus_tag="VLF19_03445"
     CDS             440..1555
                     /locus_tag="VLF19_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HSE92335.1"
                     /translation="MTTRLFCSKPFEWFEVTQLNGRGGVYLCCPSWLPVPVGNVLQQP
                     VAAIWNSPRAQQIRGSILDGSFRYCNANRCPYLQTRTGPVQSTDAVTDPDLRAAIRER
                     RTVLPYGPRKVICTYDQSCNLSCPSCRAEVLVETDAREQILRIQERLEREALAEADYL
                     HITGSGDPFGSPFFRRWLQTMDRAAMPRLERIHLHSNGLLWTRMWKTIPEDVRALIRS
                     AEISIDAARAETYALNRRGGRFADLLENLALIGGLRSSGTLDFVKISMVVQANNVVEM
                     PEFVRLGQRFGVDAVYFSQLVNWGTFTEDEFRSRAVHLPGHPRHQELIALLDDPIFDQ
                     PVVDLGNLTVTRRSRATRERGLVRWSRWLKARISSIA"
     gene            complement(1632..2726)
                     /locus_tag="VLF19_03450"
     CDS             complement(1632..2726)
                     /locus_tag="VLF19_03450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSE92336.1"
                     /translation="MQPGWLKQYFHQHVDGSCENEKTDVAAGSVSRRDFMKSGMVAGV
                     AAGAMTAEVAGPAQAQQSFKNPLSNQWWPSPWGAQDQRGACNRITPQKVLEAARLIKT
                     GKIYPLGRVLQKGIPASGERLGAHIVIPGTPTGGPFGKQELFYHDEIFVGEIGQAGSQ
                     FDGLGHIGMRAGDGKIRYYNGLTQEEVGGAYGLKKLGAEHLMPFFTRGILLDVLALKG
                     GDRLPVNYVITVDDIMRTLRRQNIKEPGEGDAVIFHTGHGKLWMKDNAKYTKSCPGPG
                     ITAGKWLAEKKVAVLGADTWPVEAVPGENPDLPLGCHTIWIPMNGIYINENLDTEALA
                     RDRVYEFAWSFNPLPLKGATGSPGNSVAIA"
     gene            complement(2808..3302)
                     /locus_tag="VLF19_03455"
     CDS             complement(2808..3302)
                     /locus_tag="VLF19_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92337.1"
                     /translation="MGARFASGIVGGLVGGVVFGVLATVMTEVVVGDGVVTLMRLVGR
                     AAGSGRLLLAWMIVLGIGALLGAIFGILVGRREHDSSAVASYGLFYGLAIWLLVGFVA
                     VPVELGVAPFAVAANPETWSWLPATLVACLLFAGALAGTFIRLRGKRAAPPVTSGRTL
                     RRAA"
     gene            complement(3602..4747)
                     /locus_tag="VLF19_03460"
     CDS             complement(3602..4747)
                     /locus_tag="VLF19_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92338.1"
                     /translation="MPSAVVPADHAGILHALLDASPLAVIGLAADGTVRLWSPAAERL
                     FGWKEEHVLGRQDPLAPGPLAGEARRLLDRALAGEPVSGVEVVRQTLDGALVTVNVWA
                     MLVGLPAPTTVRVLFLYQDVTERKRTEQQLVRQREALHRNEKLADLGRLAAGVAHELR
                     NPLAVIDTRVQLLERLLARGGEGTESLAHHVGYLEEAAARMKRIVEGLSSYARPSRVE
                     PAVLDVREVLVATRELLRAEARGQGTEIVLEVPERRVPVLGDRSRLMQILINLGTNAL
                     EAMSEHPGRLVLRACLESADDADRVVVEVSDTGPGIPPDRLESIWEPFFTTKAEGTGL
                     GLSIVRSLIAEQPGAVLTMQSALDRGTTFRLSLLAAPPPASREERLA"
     gene            5136..5381
                     /locus_tag="VLF19_03465"
     CDS             5136..5381
                     /locus_tag="VLF19_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92339.1"
                     /translation="MIGSFVIGAIAGGVAVWYYGPRIKDYVDETSRTVRSRTADTLQS
                     AAETLQSAKQAVEPGSGSGAHGAGDPGVMGSHERRIG"
     gene            complement(5450..5878)
                     /gene="dut"
                     /locus_tag="VLF19_03470"
     CDS             complement(5450..5878)
                     /gene="dut"
                     /locus_tag="VLF19_03470"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSE92340.1"
                     /translation="MRLAVHRLDPALPLPEYARPGDAGLDLRAAESVTLSPGTRALVP
                     TGIAVAIPEGYAGFVLPRSGLALRQGVTVLNAPGLIDAGYRGEIKVLLVNHDARSVTV
                     ARGDRIAQLVVQRVERAELLEADDLPGSDRGTGGFGSTGD"
     gene            5945..7144
                     /locus_tag="VLF19_03475"
     CDS             5945..7144
                     /locus_tag="VLF19_03475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92341.1"
                     /translation="MATALAGVTVLDLATFLAAPVAATLLGEFGAEVIKVEQPGVGDD
                     LRRLGRSADGRSSYWWFVEARNKKSITCNLRAPEGQALIRRLVAGAHVLTENFRPGTL
                     ERWNLGWDALAAVRPALVMVRISAFGQTGPYRERPGFGRIAAAVGGLAYLSGYPDRAP
                     VPPGTPTVPDYLAGVFGALGALVALRHAELTGQGQVVDVGLHEPVLRVLDDAIAVHGA
                     TGRVRERIGSGTDSVVPHDHYLTRDGRWLALACTNDRMFARLLGAMGRADLRHDPRMA
                     TGAARLQHRAAVDALVGDWIAAHDAAEALARLQAAEVPSSLVMSVRDLFEDPHVRARD
                     DIVHIAAGALGPLAMPAVVPRLTATPGEVRHAGPAAPGEHNEEIYGGRLGLSTADLAD
                     LRARGIV"
     gene            complement(7191..9167)
                     /locus_tag="VLF19_03480"
     CDS             complement(7191..9167)
                     /locus_tag="VLF19_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSE92342.1"
                     /translation="MNRSLLGALLACVWLAAPPTVAALTVGEAILRVKPAVVLITARV
                     DAEVTMDCGEGPVTVKPSPFVETGTGWFVDGRGWVITNAHVVDPVHRTPPWVVHDLKK
                     KAIDQACVDPALQAQGLMRGQKPEVEDRLRLRFTDRAMRGMKFAPQPSLTVMLSNGMK
                     LPAAVRKFSAPLAFDASGRPTPDAGRDLALLRVPEGTYPAIALSTREPKIGDAVHILG
                     FPGVVLYHELLNQSATIEASVNKGAISGLRPDAIGQDLLQTDASASHGNSGGPAIGDD
                     ANVVGVMMAVSLSPTGGAIVQGFNFLIPARDVLKFLAGTDVKPGDSRFTAVWVAGLSL
                     LFEERYKAAAAKLAEANAILPNLTDVKRELAEAEAKVKNPPPRPFPWAWATVGVTLVS
                     AGAYGGMFGRRWWKNRFRVQPTHIIGFIERGLNPLLVDVRTKTDYETSPLKLPGAVRL
                     DPADAEVGRIALEVDPKQLIVTYCTSPAEKTSARVAQLLRQKGYSHVRILKGGLGGWT
                     NARLPVEAKSSLPSIGLEIYKNLTLGDVERRKFKANEVIFTEGADAHGEAYVIHAGTV
                     EIRKHFDGTERLLNTHHEGDLFGEVALFRRSLRSATAVAQGDVELLIVTYERLEWLIR
                     NRPQLTMELLKRLSEWLVATDRDRSERPARVTAS"
     gene            9680..10072
                     /locus_tag="VLF19_03485"
     CDS             9680..10072
                     /locus_tag="VLF19_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSE92343.1"
                     /translation="MTKRRRVRNARGFTLIELMIVVAIIGIIAAIAFPLYAGLTGKAR
                     LTKAQADTRTIVTAVNMYWAHMETLPATLDDLTGPVTNAYGQTAGPFLATIPTPPNSA
                     WSPYTYAPGAPPVFTVTTTGEGYTVTMP"
     gene            complement(10085..12652)
                     /locus_tag="VLF19_03490"
     CDS             complement(10085..12652)
                     /locus_tag="VLF19_03490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSE92344.1"
                     /translation="MDSYRLPRHVVPTRYEIRLEPDLVTATFTGVERITVRVAEPTRE
                     VVLNAAELAVTEASVADGRGRALAGRATLEPELERCRLVFDGDVTPGEWTLTVVFSGA
                     LNDKLRGFYRSTFKDQSGATRTLAATQFEATDARRAFPCWDEPAFKAVFASTLVIDPA
                     LTAVSNTRVVAERTEGSKKVVTFADTIKMSTYLVAFVVGELEATDPVTVGRTSLRVWC
                     VPGKRRLAAFAREIGAASLRFFEDYYGLPYPGDKLDLLAIPDFAAGAMENLGAITFRE
                     TALLVDERAATHAELERIADVVAHENAHMWFGDLVTMTWWNGIWLNEAFATFMEMLAV
                     DDWKPEWRRWTSFGVSRAAAFGVDGLWSTRPIEFEVKAPRDADAMFDVLTYEKGASVL
                     RMLEQYLGREVFRAGVREYLRAHTYANADTGDLWAALGRASGADIPGVMDGWIFRPGF
                     PLVTVRRDGSTLRLAQQRFTYLPEPPPWTGADGARDQRWQVPVQVRLVAGERAETRRV
                     LLAEPEERLAAPGDLDAVLVNDGGHGFYRVRYAPDLLAPLLHRLDSLAPIERFNLVGD
                     AWAATTAGLDPVADYLEMTTRFHGERDRNVWTILLASLGVLNRIVEPAERPRLEAFVR
                     NLLAPAVAELGWAPRAGEDDLIRQLRGDLLRAIGTLGNDRAAQARADELFAAYLADAG
                     AVDPNVLPALIAIRAHTGDAVRYDDFLRRFRTATTPQEEQRYLYALAGFRASELLAQT
                     LERTINGEIRTQDAPFVVRSVLMSVYGRERAWAFVKRHWDTMDRLFPKHGLRRLCEGF
                     TGLTTPALERDVHAFVRERKISLGGRTLDQYLEQLRVAVALRERESERLVAYLARFS"
     gene            complement(12734..>13030)
                     /locus_tag="VLF19_03495"
     CDS             complement(12734..>13030)
                     /locus_tag="VLF19_03495"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HSE92345.1"
                     /translation="FFARALLAPAPPAGAWARAADEVRALARTRTHPVSATLLADLLA
                     GEGWAPLHDLAQAVAANDGAAAVVAARDLVRLGHSSGWDLLAGFLAALTGGRLL"
BASE COUNT         1919 a         4591 c         4629 g         1891 t
ORIGIN      
        1 gaggcggatc gcgtgagacg gtattgcgac ggcgtggggc tggcccccga cgtcacgttc
       61 gtggtggaaa gctcggatat cgccctgacc atcgccgggc gcgttccgga cgcgctcgat
      121 gttgtgttca tcgacggcgc ccacgggttt cctgcgccga tcctggactg gcactacacc
      181 gcgaggaggc tgcggatagg aggcctcctc ggcgtcgacg actacaagat gccctccgtg
      241 aggatcctgt acgattttct gaggcacgag gaggagtgga cgctcgagaa gatgtcctgg
      301 aacaccgcct tcttccggaa gctccgcgag ccgacggagc tggtggactg gtcgggccag
      361 cgcatcaacg ccgggtttcg cggctggtga ggcggccgct ccgcgcgccg ggcgcatcag
      421 cggacaggaa cgagtgggta tgacgacgag gttgttctgc tccaagcctt tcgagtggtt
      481 cgaggtcacg cagctcaacg gacgcggcgg cgtctatctg tgctgtcctt cctggctccc
      541 cgtcccggtc ggcaatgtcc tccagcagcc ggtggcggcc atctggaaca gcccgcgggc
      601 gcagcagatc cgcggctcga tcctggacgg ctcgttccgg tactgcaacg ccaaccgctg
      661 cccctatctc cagacgcgga ccggacctgt ccagagcacg gacgccgtga cggatccgga
      721 cctccgcgcc gcgatccggg agcggcgaac cgtccttccc tacgggcccc ggaaggtgat
      781 ctgcacctac gaccagtcct gcaacctgtc ctgcccctcc tgtcgcgccg aggtgctcgt
      841 cgagacggac gccagggagc agatcctgcg catccaggag cgtctggagc gggaagcact
      901 ggcggaggcc gactacctgc acatcacggg atcgggcgat ccgttcggca gcccgttctt
      961 ccgccgctgg ctgcagacga tggaccgggc cgcgatgccg cggctcgagc ggatccacct
     1021 gcactcgaac ggactgctgt ggacgcggat gtggaagacg atcccggagg acgtccgggc
     1081 gctgatccgc agtgcggaga tctcgatcga cgccgcccgc gcggagacct acgccttgaa
     1141 ccggcggggc ggccgcttcg cggacctgct cgagaacctc gcgctcatcg gcggattgcg
     1201 gagcagcggt acgctcgact tcgtcaagat cagcatggtg gtccaggcga acaacgtcgt
     1261 cgagatgccc gaattcgtcc gcctgggcca gcgattcggg gtcgatgccg tctacttcag
     1321 ccagctcgtc aactggggca cgttcacgga ggacgagttc cgctcacggg ccgtgcatct
     1381 ccccggccat ccacgacacc aggagctgat cgccctgctg gacgacccga tcttcgacca
     1441 gcccgtcgtc gacctgggaa acctgaccgt gacgcggcgc tcgcgggcga cgcgcgaacg
     1501 cggcctcgtt cgctggagcc ggtggctcaa ggcgcggatt tcgagcatcg cgtgaccccg
     1561 cctgcggctc cgcgctcgcg agccgggatc gggcttcgcc gcccgggcca ccggtcgatg
     1621 tcacgacggg ctcaggcgat cgcgacggag ttgcccggcg aaccagtcgc gcccttgagc
     1681 ggcagcggat tgaacgacca cgcgaactcg tagacgcggt cgcgcgccaa tgcttccgtg
     1741 tcgaggttct cgttgatgta gatcccgttc atcgggatcc agatcgtgtg acacccgagg
     1801 gggaggtcgg ggttctcgcc aggaaccgct tcgaccggcc acgtgtccgc gccgagcacg
     1861 gcgaccttct tctcggcaag ccacttgcca gcggtgatgc cgggcccggg acaactcttg
     1921 gtgtacttgg cgttgtcctt catccagagc ttgccgtgtc ccgtgtggaa gatgaccgcg
     1981 tcgccctcgc ccggctcctt gatgttctgc cggcgcagcg tccgcatgat gtcgtcgacg
     2041 gtgatgacgt aattgacagg cagacggtcg ccgcccttca acgccagcac atcgagcagg
     2101 atgccgcgcg tgaagaatgg catcaggtgc tcggcgccga gcttcttcag accgtacgcg
     2161 cccccgactt cctcttgcgt caggccgttg tagtagcgga tcttgccatc accggcacgc
     2221 atcccgatgt gacccaggcc gtcgaactgt gatccggcct gaccgatctc accgacgaag
     2281 atctcgtcgt gatagaagag ctcttgtttc ccaaacggac cgcccgttgg cgtccccggt
     2341 atgacaatgt gcgcgcccag ccgctcgccg gacgccggga tccccttctg gagcacgcgg
     2401 ccgaggggat agatctttcc ggtcttgatg aggcgtgccg cctcgagcac cttctgcggc
     2461 gtgatgcggt tgcacgcgcc gcgctgatcc tgtgcgcccc atggggacgg ccaccactgg
     2521 ttgctgagcg ggttcttgaa cgactgctgc gcctgcgccg gtcccgcgac ctccgccgtc
     2581 atcgctcccg cggcgacgcc cgcgaccatg ccgctcttca tgaaatcccg gcgcgacacg
     2641 ctgcccgcgg cgacgtcggt tttctcgttt tcgcacgagc cgtcgacatg ctggtggaag
     2701 tactgtttga gccaaccggg ttgcactgga cacctcctgg gcgggacctg agccttcggt
     2761 gcaccgacga cacgagcgac gactacccga agctggtctc caccgggtca ggcggcccgg
     2821 cggagcgtcc gtccggatgt taccggcgga gcggcacgct ttccgcgcag ccggatgaat
     2881 gtgcccgcga gcgcgcccgc gaacaggagg cacgcgacga gcgttgcagg aagccatgac
     2941 caggtctcgg gattcgccgc gaccgcgaag ggcgcgacgc ccagctcgac tggcacggcg
     3001 acgaaaccga cgagcagcca aatggctaac ccgtagaaca ggccgtagct ggcgaccgcg
     3061 ctgctgtcgt gctcccgacg cccgacgaga atgccgaaga tcgctccgag caaggcgccg
     3121 atgccaagga cgatcatcca cgccagcagc agtcgccccg agccggcagc ccggccgaca
     3181 agccgcatca aggtgacgac cccatcgccg accaccacct cggtcatgac ggtggccaga
     3241 acaccgaaca cgacaccgcc gacgagaccg cccacgattc cagacgcgaa cctggcgccc
     3301 atgatgtcct cctgccgcgg ccgcggtgct ggggcagtcg cgccgcgaga gtctcgttca
     3361 tttggcacag gttccccgat ccgcaccgcc ctcgcaagcg cggatgatcg aaagggtccg
     3421 ggcccccggg accggacgcc gaaatacacc tgcccttcga ccgcgacgaa aggtcaccgt
     3481 gggcccctcg gttctgcccg gacctgcgcg ggttcgcggg gcatcaccgg ctccacggtg
     3541 gagctctctc ccccggctgc gtacgagcgc ctccaccccg tcgggcgatc gtcggctcgt
     3601 ctcaggcgag gcgttcttcg cgcgaggccg gcggcggggc ggcgaggagc gacagccgga
     3661 aggtcgtgcc gcggtcgagc gcgctctgca tggtcaagac ggcgcccggc tgctccgcga
     3721 tgagcgagcg cacgatggac agcccgagtc cggtgccctc ggccttggtg gtgaagaacg
     3781 gctcccagat gctctcgagg cgatcggggg ggatcccggg gccggtgtcg gagacctcga
     3841 cgaccacgcg gtcggcgtcg tcggccgact ccaggcacgc gcggagcacg aggcggccgg
     3901 ggtgctcgct catcgcctcc agcgcgttgg tcccgaggtt gatcaggatc tgcatcaggc
     3961 ggctccggtc gccgagcacc ggtacccggc gctccggcac ctcgagcacg atctcggttc
     4021 cttgcccgcg cgcttcggcc cgcagcagct cgcgcgtcgc gaccagcacc tcgcgcacgt
     4081 cgagcaccgc cggctcgacg cgtgacggac gcgcgtagct cgagagcccc tcgacgatgc
     4141 gcttcatccg cgcggcggcc tcctcgaggt aacccacgtg gtgcgcgagg gactccgtgc
     4201 cctcgccgcc ccgcgcgagc aggcgctcga ggagctgcac gcgggtgtcg atcaccgcca
     4261 ggggattgcg cagctcgtgc gcgaccccgg ccgccagacg gccgaggtcg gcgagcttct
     4321 cgttgcggtg gagcgcctcg cgctggcgga cgagctgctg ctcggtccgc ttgcgctcgg
     4381 tgacgtcctg gtagagaaag agcacgcgca ccgtcgtcgg cgcgggcagg cccacgagca
     4441 tggcccacac gttcacggtg acgagggcac cgtccagggt ctggcggacg acctcgaccc
     4501 cgctcaccgg ctccccggcg agcgcacggt cgagcaggcg gcgcgcttct ccggcgagcg
     4561 gcccgggcgc cagtggatcc tggcggccga gcacgtgctc ctccttccac ccgaacagcc
     4621 gctcggccgc cgggctccag agcctgacgg tgccgtccgc cgcgagtccg atgacggcga
     4681 gtggcgaggc gtcgaggagg gcgtgcagga tgccggcgtg atcggcggga acgaccgcag
     4741 acggcatccc cgtggcactt gcgtgcgact cgatccgggg gacggccgag cgcccgggct
     4801 cgaggccgtt ggtcggagcc aggcgtcgca cctgcgtcag caccaccgcg cacagacgcc
     4861 gaaagaccgg aaccgcgagc agcgccggca cgagccggcg cgcacggtcg gtggggctga
     4921 acgttggcac tgggcagcag agacgaggca aaggcgatac cacgccgcac gcacacgata
     4981 tcaatgtgtt agggccgggc gtccctggcg cgggtgcaca gtggatggcc acctgcacac
     5041 tccgtgtcca acctcctgac gtcaacgtga tgctggcggc atcgtctttg ctcgccgctc
     5101 tctgcagcgg accaaccggc aaggaggacc agatcgtgat tggaagcttc gtcatcggcg
     5161 ccatcgcggg cggcgtggcc gtgtggtact acggcccccg catcaaggac tacgtcgacg
     5221 agaccagccg cacggtgcgg tcgcggacgg ccgacacgct ccagtcggcg gcagagaccc
     5281 tgcagtcggc gaagcaggcc gtggagccgg gaagtggatc cggcgcacac ggggccggcg
     5341 acccgggagt catggggagc cacgaacgtc ggatcgggtg acggcgggcg gctgtcagcg
     5401 ggtggcggtc agtgacggcg gtcggccggc ggccgtcggg cggcggcggt cagtctcctg
     5461 tcgagccgaa tccgccggtg ccccgatcgc tccccggcag atcgtcggcc tcgagaagct
     5521 cggcgcgctc gacgcgctgg acgacgagct gcgcgatgcg gtcgccgcgg gcgaccgtca
     5581 ccgacctggc gtcgtggttg acgagcagca ccttgatctc tccgcggtag cccgcgtcga
     5641 tcagccccgg cgcgttcagc accgtcacgc cctgccggag cgcgagcccc gagcgaggta
     5701 gcacgaagcc ggcgtaaccc tccgggatcg ccaccgcgat gccggtcggg acgagcgcgc
     5761 gggtgccggg cgacagcgtg acgctctccg cggcgcgcag gtcgagcccc gcgtcgccgg
     5821 ggcgcgcgta ctccggcagc ggcagcgcag ggtccaggcg gtggacggcg aggcgcatca
     5881 cggcgggcga gtgtagcacg cgcgccttgc gcgctttgcc ggccggggcg tagagtccgg
     5941 cacggtggcc acagccctgg ccggcgtgac cgtgctcgac ctcgcgacgt tcctcgccgc
     6001 gcccgtggcg gcgacgctgc tcggcgagtt cggcgccgag gtgatcaagg tcgagcagcc
     6061 cggcgtcggc gacgacctgc ggcggctcgg ccgctccgcc gacgggcgca gctcgtactg
     6121 gtggttcgtc gaggcgcgca acaagaagtc gatcacctgc aacctgcgcg cgcccgaagg
     6181 ccaggcgctg atccgtcggc tcgtcgccgg cgcccacgtc ctcaccgaga atttccgccc
     6241 cggtacgctc gagcgctgga acctcggctg ggacgcgctc gccgccgtgc gaccggcgct
     6301 ggtcatggtc cgcatctcgg cgttcggcca gaccggtccg taccgcgagc ggcctggctt
     6361 cggccgcatc gcggccgccg tcggcgggct cgcctacctc tcgggctacc cggaccgcgc
     6421 gcccgtgccg ccgggtacgc caacggtgcc cgactatctc gccggcgtct tcggggcgct
     6481 cggtgcgctg gtcgcgctcc gccacgccga gctcaccggg caaggacagg tggtggacgt
     6541 gggcctccac gagcccgtgc tgcgggtgct cgacgacgcc atcgcggtcc acggcgctac
     6601 cgggcgggtc cgggagcgga tcggctccgg cacggacagc gtcgtacccc acgatcacta
     6661 cctcacgcgt gacggccgct ggctggcgct cgcgtgtacc aacgaccgca tgttcgcgcg
     6721 gctgctcggg gcgatggggc gtgccgacct gcgccacgac ccgcgcatgg ccacgggcgc
     6781 cgcgcggctc cagcaccggg ccgccgtcga cgccctcgtc ggcgactgga tcgccgccca
     6841 cgacgccgcc gaggcgctgg cgcggctcca ggccgcggag gtgccgtcgt cgctggtcat
     6901 gagcgtgcgc gacctcttcg aggacccgca cgtgcgcgcg cgggacgaca tcgtgcacat
     6961 cgccgccggc gcgctggggc cgctcgcgat gccggcagtc gtgccccggc tcaccgcgac
     7021 gccgggcgag gtacgccacg cggggcccgc ggcgccggga gagcacaacg aggaaatcta
     7081 cgggggccgc ctcggactct ccaccgcgga cctggccgac ctccgcgcgc gggggatcgt
     7141 ctagacgggg catcgaggcg cgcgacccgc ctcgacggcc cccttcgacc tcaggacgcg
     7201 gtgacgcggg cggggcgctc gctccggtcg cggtcggtgg ccacgagcca ctcggacagg
     7261 cgcttgagca gctccatcgt cagctgcggc cggttgcgga tgagccattc cagccgctcg
     7321 taggtgacga tgagcagctc gacgtcgccc tgggccacgg ccgtggccga gcggagcgag
     7381 cggcggaaga gcgccacctc gccgaacagg tcgccctcgt ggtgggtgtt cagcaggcgc
     7441 tcggtgccgt cgaaatgctt gcggatctcg acggtgccgg cgtggatcac gtacgcctcg
     7501 ccgtgcgcgt cggcgccttc cgtgaagatc acctcgttgg ccttgaactt ccggcgctcg
     7561 acgtcgccga gcgtcaggtt cttgtagatc tcgagcccga tcgagggcag cgaggacttc
     7621 gcctccacgg gcaggcgcgc gttcgtccag ccgccgaggc cccccttgag gatgcgcacg
     7681 tgcgagtacc ccttctggcg cagcagctgg gcgacgcgcg cgctcgtctt ctccgccggg
     7741 ctcgtgcagt aggtgacgat gagctgcttg ggatcgacct cgagcgcgat ccggccgacc
     7801 tccgcgtctg ccgggtccag ccgcacggcg cccgggagct tgagcgggct ggtctcgtaa
     7861 tcggtcttgg tccggacgtc cacgaggagc gggttgagcc cccgctcgat gaagccgatg
     7921 atgtgcgtcg gctggacgcg gaagcggttc ttccaccacc gccgcccgaa catcccgccg
     7981 tacgcccccg cgctgaccag tgtgacgccc acggtcgccc acgcccacgg gaacggccgg
     8041 ggcggcgggt tcttcacctt cgcctcggcc tccgccagct cgcgcttcac gtcggtgagg
     8101 ttcggcagga tcgcgttggc ctcggcgagc ttcgccgcgg ccgccttgta acgctcctcg
     8161 aagaggaggc tgagcccggc gacccacacc gcggtgaacc tgctgtcgcc gggcttcacg
     8221 tcggtgccgg ccaggaactt gagcacgtcc cgcgccggga tgagaaagtt gaagccctgc
     8281 acgatggcgc cgccggtggg agagagtgag accgccatca tcacgccgac cacgttggcg
     8341 tcgtcaccga tggctggacc gccgctgttg ccgtgggagg ccgacgcgtc ggtctggagg
     8401 aggtcctgcc cgatggcgtc cggcctgagc ccggagatgg cgcccttatt gaccgaggcc
     8461 tcgatggtgg cgctctggtt gagcaactcg tgatagagca ccacacccgg gaagcccagg
     8521 atgtgcacgg cgtcgccgat cttcggctcg cgggtggaca gcgctatggc gggataggtg
     8581 ccttcgggca cgcgcagcag cgccaggtca cgcccggcgt ccggggtggg ccgtccgctg
     8641 gcatcgaatg caagcggagc gctgaacttc cgcaccgccg ccggaagctt catgccgttg
     8701 gacagcatca ccgtgagcga cggctgcggt gcgaacttca taccgcgcat ggcgcggtcg
     8761 gtgaaccgga ggcggagccg atcctcgacc tcgggtttct gaccgcgcat gagcccctgc
     8821 gcctggagcg cggggtcgac gcacgcctgg tcgatggcct tcttcttcag gtcgtggacg
     8881 acccacgggg gcgtccggtg cacggggtcc accacgtggg cgttggtgat gacccagcca
     8941 cggccatcga cgaaccagcc cgtccccgtt tcgacgaagg gcgagggctt gacggtgacc
     9001 gggccctcgc cgcagtccat cgtcacctcg gcatccacgc gggcggtgat gagcacgacg
     9061 gcgggcttga cccgcaggat cgcctcgccc accgtgagcg cggcgacggt cggcggcgcg
     9121 gcgagccata cgcaggccag cagggcgccg agtagcgagc gattcatccc tggtcacggt
     9181 atcacggagc ctcgaccctt tgcaccccgc gtgagttccg ccacgcgcgg cgactgtctc
     9241 cggcccgccc gccagaggaa ccgcccatgt cacgatcgct gatcgcttct ctgaccggcg
     9301 cgcgcgtgcc gggcatcgag tggagacgct gcgcgagcag gtcgtcgaca tcgacttcgg
     9361 caaccggcgc ggtggcgtgg cgtccgcggg catcgccgcc cgaccgaagt cgctgcccca
     9421 cgcgatccac cgcatggcga agtggccggt gtggaaggca cgatcctcgg gaccttgcgg
     9481 ggtggatccg agcccgacgg tgtgggtgac gttccctgtc agtccgcccc cacgttctgg
     9541 cattcggccc caccgtcacc gatagtggat gatccctaat tgcttgattc cgttaggtgt
     9601 gtgtgggctg gcaccagagc tgcaggcatc aggccctgaa gaagtggagg cggatcggac
     9661 gcgacaaggg aggggcgccg tgacgaagcg ccgacgggtt cgcaacgcgc ggggcttcac
     9721 gttgatcgag ttgatgatcg tggtggcgat catcggcatc atcgccgcca ttgcatttcc
     9781 gctctacgcc ggcctcacgg gcaaggcgcg gctcaccaag gcgcaggcgg acacgcgcac
     9841 catcgtcacc gcggtgaaca tgtactgggc gcacatggag acgttgccgg cgaccctcga
     9901 cgacctcacc gggcccgtga cgaacgccta cggccagacg gccgggccgt tcctggcgac
     9961 gattccgacc ccgcccaact cggcgtggtc gccctacacg tacgcgccgg gcgcgccccc
    10021 ggtcttcacg gtcaccacca cgggcgaagg ctacacggtc acgatgccct gagggcgccg
    10081 gctctcagct gaaccgcgcc agataggcca ccagccgctc gctctcgcgc tcgcgcaggg
    10141 cgacggcgac gcggagctgc tcgaggtact gatcgagcgt cctgcctccc aggctgatct
    10201 tccgctcccg gacgaacgcg tgcacgtcgc gctcgagcgc gggcgtggtc agcccggtga
    10261 acccctcgca caggcgccgg aggccgtgct tggggaacag gcggtccatc gtgtcccagt
    10321 ggcgcttgac gaacgcccac gcccgctcgc gtccgtacac gctcatgagc acgctccgca
    10381 cgacgaaggg ggcgtcctgt gtgcggatct ccccgttgat cgtgcgctcg agcgtctggg
    10441 cgagcagctc cgaggccctg aagccggcca gcgcgtacag ataccgctgc tcctcctgcg
    10501 gcgtcgtcgc ggtccggaaa cggcggagga aatcgtcgta gcggacggcg tcgccggtgt
    10561 gcgcgcggat ggcgatcagc gccggtagca cgttgggatc gacggcgccc gcgtccgcga
    10621 ggtaggccgc gaacagctcg tcggcccgtg cctgggcggc gcgatcgttg cccagggtgc
    10681 cgatcgcgcg caggaggtcc ccgcggagct ggcggatcag atcgtcctcg ccggcgcgcg
    10741 gcgcccagcc gagctccgcg acggccggcg cgagcaggtt gcgcacgaac gcctcgagcc
    10801 gcgggcgctc cgcgggctcg acgatgcggt tcaggacccc cagcgaggcg agcaggatcg
    10861 tccacacgtt gcggtcgcgc tcgccgtgga agcgcgtcgt catctcgagg tagtcggcca
    10921 cggggtcgag acccgcggtc gtcgccgccc aggcgtcgcc caccaggttg aagcgctcga
    10981 tcggcgccag tgagtcgagg cgatgcagca gcggcgcgag caggtccggc gcgtagcgca
    11041 cgcgatagaa gccgtgaccg ccgtcgttca cgaggaccgc gtcgagatcg cccggcgccg
    11101 cgaggcgctc ctcgggctcc gcgagcagca cgcgccgcgt ctcggcgcgc tcgccggcca
    11161 ccaggcggac ctgcacgggc acctgccacc gctggtcgcg ggcgccgtcg gcgcccgtcc
    11221 acggcggcgg ctccggcagg tacgtgaagc gctgctgggc cagccggagc gtgctgccgt
    11281 cgcggcgcac cgtcaccagc gggaagcccg gccgaaagat ccagccgtcc atcacgccgg
    11341 ggatgtccgc gccggacgcg cgcccgagag cggcccacaa gtcgccggtg tcggcattgg
    11401 cgtaggtgtg ggcacgcagg tactcgcgaa ccccggcgcg gaacacctcc cgtccgaggt
    11461 actgctcgag catgcggagc acggaagcgc ccttctcgta cgtgagcacg tcgaacatcg
    11521 cgtcagcgtc gcggggcgcc ttgacctcga actcgatggg gcgcgtggac cagagcccat
    11581 ccacgccgaa ggcggccgca cgggagaccc cgaacgacgt ccaccgccgc cactcgggct
    11641 tccagtcgtc gacggccagc atctccatga aggtggcgaa cgcctcgttg agccagatgc
    11701 cattccacca cgtcatggtc acgaggtcgc cgaaccacat gtgggcgttc tcgtgggcga
    11761 cgacgtcggc gatacgctcc agctccgcgt gagtcgccgc ccgctcgtcg accaggagcg
    11821 cggtctcgcg aaaggtgatg gcgccgaggt tctccatggc gccggccgcg aagtcgggga
    11881 tggcgagcag gtcgagcttg tcgcccgggt agggcaggcc gtagtagtcc tcgaagaacc
    11941 gcagcgaggc ggcgccgatc tcccgcgcga acgccgcgag ccgccgcttg ccgggcacgc
    12001 accacacgcg cagcgacgtc cggccgaccg tcaccgggtc ggtcgcctcc agctcgccga
    12061 cgacgaacgc gacgaggtac gtggacatct tgatcgtgtc cgcgaacgtc acgaccttct
    12121 tgctgccctc cgtgcgctcg gcgaccaccc gcgtgttcga gacggcggtg agcgcggggt
    12181 cgatcaccag ggtgctggcg aacacggcct tgaaggcggg ctcgtcccag caggggaacg
    12241 cgcgccgggc gtcggtcgcc tcgaactgcg tggcggcgag cgtgcgggtc gcgccgctct
    12301 ggtccttgaa cgtgctgcgg tagaagccgc gcagcttgtc gttcagcgca ccggagaaca
    12361 cgacggtgag ggtccactcg ccgggggtga cgtcgccgtc gaagaccagc cggcagcgct
    12421 cgagctcggg ctccagcgtg gcgcgaccgg ccagcgcgcg gccgcgcccg tcggcgacgg
    12481 atgcctcggt caccgccagc tcggcggcgt tgaggacgac ctcgcgcgtc ggctcggcca
    12541 cgcggacggt gatccgctcc acgccggtga aggtcgccgt cacgagatcg ggctcgaggc
    12601 ggatctcgta gcgcgtgggg acgacgtgac ggggcaggcg ataggagtcc acgaaatcct
    12661 ccggcgtcgc tgggatctgg ggtgcgcgcc cgtgccgccg gcgcgcggct cacgctgcta
    12721 ctctaccgca ggctcaaaga aggcgccccc cggtgagcgc ggcgaggaat ccggcgagga
    12781 gatcccagcc cgaggagtgg ccgaggcgca cgaggtcgcg cgccgccacg accgccgccg
    12841 cgccgtcgtt ggccgccacg gcctgcgcca gatcgtgcag cggcgcccag ccctcgcccg
    12901 cgagcaggtc ggcgaggagc gttgcgctca cgggatgcgt gcgcgtgcgg gccagggcgc
    12961 gcacctcgtc ggcggcgcgc gcccacgcgc cggcgggcgg cgccggggcg aggagcgcgc
    13021 gcgcgaagaa
//
LOCUS       DATIAZ010000101         6959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1603675, whole genome shotgun sequence.
ACCESSION   DATIAZ010000101 DATIAZ010000000
VERSION     DATIAZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603675"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3426)
                     /locus_tag="VLF19_03500"
     CDS             complement(<1..3426)
                     /locus_tag="VLF19_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSE92346.1"
                     /translation="MLPFEELMGESAPIAEVRDRATRLLKSTAGAGRRLPPILILGET
                     GTGKGLLAGAIHRASGRASGPFVDVNCAAIPETLLEAELFGFERGAFTDARQAKAGLF
                     QAANGGTIFLDEVGLLPTALQSKLLKVIEERSVRRLGSTRSEPLDLCVTAATSEDLAA
                     AVQEGRFRADLYHRLAVVTLELPPLRTRGRDVLLLAEHFLRRTCEDYGLSARRLAEDA
                     RAALLAHDWPGNVRELANVLERAALLGDGPLLTAAHLGLRAQPRRASPGAAAAPSEDA
                     TVEGERRLLLETLRATDWNFTRAAARLGLPRNTLRYRAERLGLAPEGPPERRRGGRPP
                     AERRAAPAPARAVSEAPRETCRVTLLQARLLPAGIEPADWNTGRALEATAAKVRSFGG
                     RIEELAPSGVLATFGLELDEDAPRRAAHAAVAVQTLAARAQSDVVHAPGVKIALHTAT
                     MPVTFEGDAVDVDVAAMLEARRTLDVLLEAAEPGSVVATAATGRFLARRFDLLPLPTV
                     DAAIAGAFRVVRHSDPGRTRFVGRERELRLLTERFELAQAGQGQVVTIVGEAGIGKSR
                     LLHEFRRRLGRSATWVEGHALSFGRAMPFHAVIDMLRRVFRVGDADTEAAIVDKLERG
                     VRRLGSDMGEALPFVRYLLSVDPGDPAVLAMDPKLRHAAIVRASHLLLERGAELRPHV
                     VVLEDMHWCDPATEDWITRLADGIAGKRVLVLVTCRPGYRPPFGGRSFHTGLALATLS
                     VEDSVSVARGLLGVEELPPALQTLVVDKAEGNPFFIEELVRSLEELGAVRRAGERLVV
                     SSRLDQVAVPDTIEEVVLARIRRLEEPLQRLLEVASVIGKNVPFPLLRAVTAQPDEKL
                     SADLDRLQAAEFLYETRAFPELEHTFKHALTHDVAYGSLKAAERRDLHARIVRATESL
                     YRNRLGEHVERLAHHAVRGELWAEAVRYSRQAGAKAFDRSANREAVASFEQTLVALAR
                     LPERRETLETAIDVRLALRSALLQLGELRKITQYLREAESLATALDDPRRLAWVWTYM
                     TTAYVLAGDPARALAVGERALAVAEEVGDLGLRATARTPLAHACCESGAYRRAIDLLS
                     ESIRALDGDLARERFGQAMPPSLYARSVSAICRAELGEFDEARMLGTESARLAE"
     gene            3554..4549
                     /locus_tag="VLF19_03505"
     CDS             3554..4549
                     /locus_tag="VLF19_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE92347.1"
                     /translation="MRSIDIHAHLTPQCFWQATENGGDWHTIRREKDARGADVAVVGS
                     KSQPLPPRAKWTPEERLADMDSLGVDVHVVSPYVGFYNYHLDTPVAVATSRATNDEIA
                     GMVRTWPQRFAGLGTLPMQDVKAAVSELERCMTQLGLKGVEINDHVNGRTLEEPEYRS
                     FWRAAEQTGALVFFHQGGETLVSQRSRRYHLPNSIGNLVDRAVTFATLVHGGVMDECP
                     DLKIVLGHGGGYTCYGIGRMDHAWKVRSEARVHITRPPSAYLRKFYYDCIVYTEEALR
                     FLIDTVGADRVVFGTDWPYDMALDWPVSWVLGMKSLTQEEKDAILSRNLERLLNL"
     gene            4576..5457
                     /locus_tag="VLF19_03510"
     CDS             4576..5457
                     /locus_tag="VLF19_03510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSE92348.1"
                     /translation="MDIGCQLPMVPTREALTTFAGEAERHRLASLWVSDHVVIPHSSS
                     GYPGGRFPHPPDKPYLEAVTVLAAAAMCTTHARLGASVFVLGHRHPVLMAKMLSTIDA
                     LSGGRLICGVGVGWWKQEIEALGSPFHARGRQADEMLRVFKELWTADDPRFEGEFYRF
                     RDLGFAPRPVQKPHPPLWVGGDSPGAFRRVVALGDGWHASGKTPAEMRDRLARIRAIA
                     DGARRPWESITLSLRFPLTDALLQQGPQAVVDLFGEYKRLGLAHLMVDFRRDELGRML
                     EVLDLLATKIRPAVDAA"
     gene            5465..>6959
                     /locus_tag="VLF19_03515"
     CDS             5465..>6959
                     /locus_tag="VLF19_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HSE92349.1"
                     /translation="MRPFTGKEFLESLRDGREVWIYGDRVSDVTTHPAFRNSARMLAR
                     LYDALHEPATRPVLTCPTDTGSGGYTHRYFRAPTSADDLVAARDAIAAWARMTYGWMG
                     RSPDYKAAFLATLGANADFYAPHQDNARRWYRNAQERVLFLNHAFVQPPVDRHRPPDE
                     VADVYVHVERETDAGLVVSGAKVVATGSALTHANFIAHPSTTPIKTKQFAAVFIATMD
                     TPGVKLICRPSYAMTAEVMGSPFDYPLSSRMDENDAILVLDRALVPWENVLVYEDVGK
                     SNTFFQNSGFFPRAMLHGCTRLAVKLDFIAGLMLKAVDAVGSTDARHVQASVGEAIAW
                     RNLFWALTDAMARAPVRWAGDTVLPNPEHAQAYRLFSTIAYPRVKEITESILASGLIY
                     LNSHAVDFKSPALRPYLDKYLRGSDGTGAVERIKLMKLLWDATGTEFGGRHELYERNY
                     AGAPETIRMLTWQMAHDSGRTREFRGFAEQCMAEYDLEGWTVPDLITP"
BASE COUNT         1001 a         2581 c         2437 g          940 t
ORIGIN      
        1 ctcggccagg cgcgccgact ccgtgccgag catccgagcc tcgtcgaact cacccagctc
       61 ggcgcggcag atcgcggaca cgctgcgcgc gtacagcgag ggcggcatgg cctgcccgaa
      121 tcgctcgcgc gcgaggtcgc cgtcgagcgc acggatggac tcggagagca gatcgatcgc
      181 ccggcggtag gctccgctct cgcagcacgc gtgggcgagc ggcgtcctcg ccgtggcccg
      241 aaggccgaga tcgccgacct cctcggcgac ggcgagggca cgctcgccga cggccagcgc
      301 gcgagccgga tcgccggcga ggacataggc tgtcgtcatg tacgtccaca cccaggccag
      361 acgccgcggg tcgtcgagcg cggtggcgag cgactccgcc tcgcgcaggt actgggtgat
      421 cttccggagc tcaccgagct gcagcagggc gctccgcagc gcgaggcgga cgtcgatggc
      481 cgtctccagc gtctcgcggc gctcgggcag gcgcgcgagc gcgacgaggg tctgctcgaa
      541 ggacgccacc gcctcccggt tcgccgatcg atcgaaggcc ttcgctcccg cctggcgcga
      601 gtagcggacg gcctccgccc acagctcccc acggaccgca tggtgggcca gccgctcgac
      661 gtgctcgccg aggcggttgc gatacaggct ctccgtcgcc cgcacgatcc gcgcgtgcag
      721 gtcgcggcgc tccgcggcct tgagactgcc gtaggcgacg tcgtgcgtga gcgcgtgctt
      781 gaaggtgtgc tccagctcgg ggaaggcgcg ggtctcgtaa aggaactccg cggcctgcag
      841 gcggtcaagg tcggcgctga gcttctcgtc cggctgcgcg gtgaccgcgc ggaggagggg
      901 aaagggcacg ttcttgccga tgacggacgc gacctcgagc aggcgctgca ggggctcctc
      961 gagccgccgg atgcgcgcga gcacgacctc ctcgatggtg tcgggcaccg ccacctggtc
     1021 caggcgggag ctgaccacca gacgctcgcc ggcacggcgc acggccccca gctcctccag
     1081 cgaccggacc agctcctcga tgaagaaggg gttgccttcg gccttgtcca cgacgagggt
     1141 ctgcagcgcc ggcggcagct cctccacgcc gaggagaccg cgggccacgc tgacgctgtc
     1201 ctcgaccgac agtgtggcca gggccagtcc cgtgtggaag ctgcggcccc cgaacggcgg
     1261 ccggtagccg ggccggcacg tcacgaggac gaggacacgc tttccggcga tcccgtcggc
     1321 gaggcgcgtg atccagtctt ccgtcgccgg gtcgcaccag tgcatgtcct cgagcacgac
     1381 cacgtgcgga cgtagctcgg cgccccgctc gagcaacagg tgggaggccc tcacgatcgc
     1441 ggcgtgccgg agcttggggt ccatcgcgag cacggccgga tcgccgggat cgaccgagag
     1501 gaggtaccgc acgaacggca gtgcctcgcc catgtcggag ccgaggcgcc gcaccccacg
     1561 ctcgagcttg tcgacgatgg cggcctcggt gtccgcgtcc ccgacccgga acacgcggcg
     1621 cagcatgtcg atgacggcgt ggaacggcat cgcgcgcccg aacgagaggg cgtggccttc
     1681 gacccaggtg gcgctccggc cgagccggcg gcggaactcg tgcaggagtc gggacttgcc
     1741 gatccccgcc tcgccgacga tcgtcacgac ctgcccctgc ccggcctggg ccagctcgaa
     1801 gcgctcggtg aggaggcgca gctcacgctc ccggccgacg aaccgcgtgc gaccgggatc
     1861 cgagtgccgg acgacgcgga aggcgcccgc gatggcggcg tccactgtcg gcagcggcag
     1921 caggtcgaag cggcgagcaa gaaatcggcc cgtcgcagcc gtggcgacca cgctgccggg
     1981 ctcggcggcc tccagcagca catcgagcgt gcgccgcgcc tccagcatcg cggccacgtc
     2041 gacgtccacg gcgtcgccct cgaaggtcac cggcatcgtc gccgtgtgca gggcgatctt
     2101 cacgccgggc gcgtgcacga cgtcgctctg cgcccgcgcc gccagcgtct ggacggcgac
     2161 cgcggcgtgg gcggcgcggc gcggggcgtc ctcgtccagc tcgaggccga acgtggccag
     2221 cacgccgctc ggtgccagct cctcgatcct cccgccgaag ctccgcacct tggccgccgt
     2281 ggcctccagc gcccggcccg tgttccagtc ggcgggctcg atccccgccg gcagcagccg
     2341 cgcctggagc agggtcaccc ggcacgtctc gcggggcgcc tcgctcacgg cgcgcgccgg
     2401 ggccggcgct gcgcgccgct ctgccggggg ccggccgccg cggcggcgct cgggcgggcc
     2461 ctccggcgcg agcccgaggc gctccgcgcg atagcgcagc gtgttgcgag gcagcccgag
     2521 ccgcgcggcg gcgcgggtga aattccagtc ggtcgctcgc agggtctcga ggagcaggcg
     2581 tcgctccccc tccaccgtgg cgtcctcgga cggcgccgcg gcggcacccg gcgatgcgcg
     2641 tcggggctgc gcgcgcaatc ccagatgagc cgcggtcagc aatggcccgt cgcccagcaa
     2701 cgcggcgcgc tcgaggacgt tcgccagctc acggacgttg cccggccagt cgtgggccag
     2761 gagcgccgcc cgcgcgtcct cggccagccg gcgggcggac agcccgtagt cctcgcacgt
     2821 ccgccgcagg aagtgctcgg ccagcagcag gacgtcgcgg ccgcgcgtcc tgagcggcgg
     2881 cagctcgagc gtgaccacgg ccagccggtg gtagaggtcc gcgcggaacc ggccctcctg
     2941 caccgccgcg gccaggtcct cgctggtcgc agcagtcacg cagaggtcga ggggctcgct
     3001 gcgcgtgctg ccgagccggc gcacggaccg ctcctcgatg accttcagca gcttcgactg
     3061 cagggcggtc ggcagcaggc ccacctcgtc caggaagatc gtgccgccgt tggcggcctg
     3121 gaagagcccg gccttggcct ggcgcgcgtc ggtgaacgcg ccccgctcga agccgaacag
     3181 ctcggcctcc agcagggtct ccgggatcgc cgcgcagttg acgtccacga acgggcccga
     3241 cgctcgcccg ctcgcgcggt ggatggcccc agccagcagc cccttgcccg ttccggtctc
     3301 gccgagaatg aggatcggcg gtaagcggcg cccggcgccg gccgtggact tcaggagccg
     3361 ggtcgcccgg tcgcgcacct cggcgatcgg cgcgctctcg cccatgagct cttcgaaggg
     3421 cagcatgtcg ccgccatcgt acccgcaacc ggccggagcg tgaaccggag cctgttgtct
     3481 tgccgcggcg atgcccctat gctgtcgcgg catccggtgc gaagggaccg gcatcgtgac
     3541 ggaaaggagc ggcatgcgga gcatcgacat ccacgcccac ctcacacccc agtgcttctg
     3601 gcaggcgacc gagaacggcg gcgactggca cacgatccgg cgcgagaagg acgcgcgggg
     3661 agcggacgtc gccgtcgtcg gcagcaagtc ccagccgctg ccgccacgcg ccaagtggac
     3721 gcccgaggag cgcctggccg acatggactc gctcggcgtc gacgtccacg tggtctcgcc
     3781 ctacgtgggg ttctacaact atcacctcga cacgcccgtg gcggtggcga cctcgcgggc
     3841 gaccaacgac gagatcgccg gcatggtccg cacgtggccc cagcgcttcg ccggcctcgg
     3901 cacgctgccc atgcaggacg tgaaggcggc ggtcagcgag ctcgagcggt gcatgacgca
     3961 gctcgggctc aagggcgtgg agatcaacga ccacgtcaac ggccgcaccc tggaggagcc
     4021 cgagtaccgg tccttctggc gggccgccga gcagacgggc gccctcgtct tcttccacca
     4081 gggcggcgag acgctggtca gccagcggag ccgccggtat cacctgccca acagcatcgg
     4141 caacctcgtg gaccgcgcgg tgacgttcgc cacgctcgtg cacggcggcg tgatggacga
     4201 gtgccccgat ctcaagatcg tcctcggcca cggcggcggc tacacctgct acgggatcgg
     4261 ccgcatggac cacgcctgga aggtgcgctc cgaggcgcgt gtccacatca cccggccgcc
     4321 gagcgcgtat ctgaggaagt tctactacga ctgcatcgtg tacacggagg aggcgctgcg
     4381 cttcctgatc gacaccgtgg gcgcggaccg cgtggtgttc ggcaccgact ggccctacga
     4441 catggcgctc gactggcccg tctcctgggt cctcggcatg aagagcctca cgcaggagga
     4501 gaaggacgcc atcctctcga ggaacctgga gcggctgctg aatctctgac ctcgacggcc
     4561 cgaaaggagc accccatgga catcggctgt cagcttccga tggtgccgac gcgggaggcc
     4621 ctcaccacgt tcgcgggcga ggccgagcgg caccgcctgg cgtcgctctg ggtcagcgac
     4681 cacgtggtca tcccccacag ctcgtccggc tacccgggcg gccgcttccc gcacccgccc
     4741 gacaagccct acctcgaggc cgtgaccgtg ctcgccgcgg cggcgatgtg caccacccat
     4801 gcccgcctcg gggcgtcggt gttcgtcctg gggcaccgcc atcccgtcct gatggccaag
     4861 atgctgagca cgatcgacgc gctgtccggc ggacgcctga tctgcggcgt gggcgtcggc
     4921 tggtggaagc aggagatcga ggctctcggg tcgccgttcc acgcccgcgg ccgccaggcc
     4981 gacgagatgc tgcgtgtctt caaggagctg tggacggcgg acgatccgcg cttcgagggt
     5041 gagttctacc gcttccgtga tctgggcttc gcgcccaggc cggtgcagaa gccgcacccg
     5101 ccgctctggg tcggcggcga cagcccgggc gccttccgcc gggtcgtggc cctcggcgac
     5161 ggctggcacg cgtcgggcaa gacgcctgcc gagatgcggg accgcctcgc gcgcatccgg
     5221 gccatcgccg acggggccag gcgcccctgg gagtccatca cgttgtcgct ccgcttcccg
     5281 ctgaccgacg cgctcctcca gcagggcccg caggcggtcg tggatctgtt cggcgagtac
     5341 aagcgcctcg gtctggcgca cctgatggtc gacttccggc gcgacgagct cgggcggatg
     5401 ctcgaggtgc tcgacctgct cgccaccaag atccgccccg cggtcgacgc ggcctgaggc
     5461 ggccgtgcgt cccttcaccg gcaaggagtt cctcgagagc ctccgggacg ggcgcgaagt
     5521 gtggatctac ggcgaccggg tgtccgacgt caccacgcat ccggccttcc gcaactcggc
     5581 gcggatgctg gcgcgcctct acgacgccct gcacgagccg gccacccggc ccgtgctgac
     5641 gtgtccgacc gacaccggct cgggcggcta cacgcaccgg tacttccgcg cccccacgag
     5701 cgccgacgat ctcgtcgccg cgcgcgacgc catcgccgcg tgggcgcgca tgacgtacgg
     5761 ctggatgggc cgctcgcccg actacaaggc ggcctttctc gccacgctcg gcgccaacgc
     5821 cgacttctac gcgccgcacc aggacaacgc ccgccgctgg taccgcaacg cccaggagcg
     5881 cgtcctcttc ctcaaccacg cgttcgtgca gccgccggtc gatcgccatc ggcccccgga
     5941 cgaggtcgcc gacgtctacg tgcacgtgga gcgagagacc gacgccggcc tggtcgtgag
     6001 cggggcgaag gtcgtggcga cggggtcggc gctgacccac gccaacttca tagcccaccc
     6061 gtcgaccacg cccatcaaga cgaagcagtt cgcggccgtg ttcatcgcca ccatggacac
     6121 cccgggcgtc aagctgatct gccgcccctc ctacgccatg acggccgagg tgatgggcag
     6181 cccgttcgac tacccgctct cgagccgcat ggacgagaac gacgcgatcc tggtcctcga
     6241 ccgggcgctc gtgccctggg agaacgtcct cgtgtacgag gacgtcggga agtcgaacac
     6301 gttcttccag aactccggct tcttcccgcg cgccatgctc cacggctgca cgcggctcgc
     6361 cgtgaagctc gacttcatcg ccggcctgat gctcaaggcc gtggacgccg tcggctcgac
     6421 cgacgcccgc cacgtgcagg cgtcggtcgg cgaggcgatc gcctggcgca acctcttctg
     6481 ggccctcacc gacgcgatgg cgcgggcgcc ggtccgctgg gccggcgaca ccgtgctgcc
     6541 gaaccccgag cacgcgcagg cctaccggct cttctcgacc atcgcctacc cgcgcgtgaa
     6601 agagatcacc gagagcatcc tggccagcgg gctgatctac ctgaactcgc acgccgtcga
     6661 cttcaagtct cccgcgctcc gtccctatct ggacaagtac ctgcgcgggt cggacggcac
     6721 cggcgccgtc gagcgcatca agctcatgaa gctcctgtgg gacgcgaccg gcaccgagtt
     6781 cggcggccgg cacgagctgt acgagcgcaa ctacgccggc gcccccgaga cgatccggat
     6841 gctcacctgg cagatggccc acgacagcgg ccggactcgc gagttccgcg gcttcgccga
     6901 gcagtgcatg gccgagtacg acctggaggg ctggaccgtc cccgacctca tcacgccgg
//
LOCUS       DATIAZ010000102         5008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_288419, whole genome shotgun sequence.
ACCESSION   DATIAZ010000102 DATIAZ010000000
VERSION     DATIAZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288419"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VLF19_03520"
     CDS             complement(<1..221)
                     /locus_tag="VLF19_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSE92350.1"
                     /translation="MSLDPVLPVFFDEAEELLRVFEEGVLELEATPHDREVLDSIFRV
                     AHTLKGSSRMLGFERIATLAQALEALLARL"
     gene            568..1857
                     /locus_tag="VLF19_03525"
     CDS             568..1857
                     /locus_tag="VLF19_03525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92351.1"
                     /translation="MRANTTPGPRILIVDDDPVMRRTLGDTLTRAGFEVAPAATVAEA
                     LRLAADAEPALAILDLVLPDGDGIELLGSLRATWPALPAIVVTAYVEPRSVVEAMRRG
                     ALDYLGKPIDPEMLLSACRAALARRPAPVTVTGKAVELPIVGVSAEIARIRETLTRLA
                     RTRPAGLLIAGPAGSGKTWLAQVLHGASPRAQAPCVVYRCVEAYAPMTALFGTPGVSG
                     SGLLAATQGGTLILDDVDRLEREVQVALLERADRQRATGAPLLVGLTEAERADGPLVA
                     WLSRVRLDVPALADRTADVIPLARHFLAEAARRLGKTIPGFTTDAEHRLLAHAWPGNI
                     RELREVVERAAAAGVGAIRPELLVLGGTATAPAWFPTGPPRPLREIEDAYIDHVIALS
                     GGNKTRAAQLLGIARETLRTRMLSRGNPGVSGEERSA"
     gene            1862..2230
                     /locus_tag="VLF19_03530"
     CDS             1862..2230
                     /locus_tag="VLF19_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92352.1"
                     /translation="MKILTVDDSDVMRRIIKHGLKAIGYDEVIEAENGQVGLARIQSE
                     AVDFVITDCSMPVMSGLEMVTAIRSNPATKDLPVLMITAPDRQEEIVQAVRAGVSGYI
                     LKPFAAETLHEKMEQVLGRA"
     gene            2227..3357
                     /locus_tag="VLF19_03535"
     CDS             2227..3357
                     /locus_tag="VLF19_03535"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HSE92353.1"
                     /translation="MSTLRRSEGHRRTRAPRGIVTTTTVAREMRDLLGGIEMCATLIA
                     RHCAGEDTVAPLTGRLLGGVARLREASANLLSLGSRSVAPAGPAPDDEAPGARVKTVR
                     RRARVAGRRARVVTVTSGKGGVGKTTLVANLGLELARLGRRVLLLDGALGLANLAILF
                     NQTPKRTLEDALARRCSVDDVVLQVREGLGIIPSAPGATRLAELEPAERASLLGEIVR
                     LGAEADVLLIDTGAGISPIVQEMVGLADRAFLVTTHEPTALSDAYGLLKAVSTGPAGG
                     SPRIDVVVNMARSHAHALDTHGRLGQLTERFLGFTPELVAVVPRDEAVGEAIVRQEPL
                     TRIYPYAPATRAIVAFARLLANPRGPDHANAHSTPLAVPVSR"
     gene            3311..4147
                     /locus_tag="VLF19_03540"
     CDS             3311..4147
                     /locus_tag="VLF19_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HSE92354.1"
                     /translation="MPTHTAHRWPFQSAGKTIRPEAVEAAWQEYRATRSADARETLVQ
                     RYLHLVRYMAGRLSRTLPPSVEFDDLVSSGLVGFLSALDSFDPDHGTDFSVYALTRIR
                     GAMVDFVREIDPIGRVTRRKLRAVERTLVQLEQELSRPPSATETAARLGITTDTYHEL
                     MSQATAAMTLSLERIQSDGDDDGVPGTRAWVADTATKDPLTTLADKDDVGRLAGLVGT
                     LSSAQQLVLHLHYVEELNFREVAMAMDISESRATQLHTAAVLALRSQARGCAVASGRT
                     GG"
     gene            complement(4477..>5008)
                     /locus_tag="VLF19_03545"
     CDS             complement(4477..>5008)
                     /locus_tag="VLF19_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92355.1"
                     /translation="LDAHCRQIGDLLNELRHFWSGGGVRAVVSLDALVERLLHLREPR
                     MRARGMRVERVLAGQLPAVAADGAQLERAVLALVLEAETAVRSRGLVRLTTGARGDSA
                     GPWVTLAVEDDGPSIPADVLPHVFDPFVPRREGRGPSVGLAAAHAIVTAHGGRLTAAN
                     RLYGGVTFLIELPAVR"
BASE COUNT          717 a         1871 c         1776 g          644 t
ORIGIN      
        1 agtcgcgcga gcagcgcctc gagcgcctgg gccagggtgg cgatgcgctc gaagccgagc
       61 atcctgctgc tgcccttgag ggtgtgggcg acccggaaga tcgagtccag gacctcgcgg
      121 tcgtggggcg tcgcctcgag ctcgaggacg ccctcctcga agacgcggag cagctcctcg
      181 gcctcgtcga agaagaccgg gagcacaggg tccagactca tggactgact ctcctgatcg
      241 cgcatcacga gatcggccgg gccaggatcc tcgcgctcag ggcctcgccc tcctcgtcgg
      301 gccctctgcc ggagtcatcg gcggtgtgcc cggctcgggc acacccgcca attgacggag
      361 gacgtagtgc agtcgcgcca gggctgcgcc gcgggatgac gccccggcac gcgaggtcgc
      421 gactccgtag cgccaacgat ctagcggcgc gcccgatcgc gtcggcgatg ggcgaagacg
      481 accccgcgtg gcgagtccgc gtatcgggac cggaatcggg ggcagcggct acgtgcgcgg
      541 acgctacatt gccagcgagg agacgacatg cgagccaata caacgccggg accgcgaatc
      601 ctgatcgtcg acgacgatcc cgtcatgcgc cggacgctcg gcgacacgct cacgcgcgcc
      661 ggcttcgagg tcgcacccgc agcgacggtc gccgaggcgc tgcggctcgc ggcggacgcc
      721 gagcccgcgc tcgcgatcct cgacctcgtg ctgcccgacg gcgatggcat cgagctgctg
      781 ggcagcctgc gcgccacgtg gcctgcgctc cccgcgatcg tcgtcaccgc ctacgtcgag
      841 ccacgctccg tggtcgaggc gatgcgacgg ggggcgctcg actacctcgg caagccgatc
      901 gatcccgaaa tgctactgtc ggcatgccgc gccgcgctcg cgcgccggcc cgccccggtc
      961 accgtcaccg gcaaggcggt ggagctgccc atcgttggcg tgagcgcgga gatcgcgcgc
     1021 atccgtgaga cgctcacccg cctcgcgcgc acccgcccgg ccggcctgct gatcgccggg
     1081 ccagcgggct ccggcaagac atggctcgcg caggtgctgc acggcgcgag cccgcgagcg
     1141 caggcgccgt gcgtcgtgta tcgctgcgtc gaggcctacg cgccgatgac ggcgctgttc
     1201 ggcacgccgg gcgtcagcgg ctcggggctc ctcgccgcga cacagggcgg cacgctgatc
     1261 ctggacgatg tggatcggct cgagcgcgag gtccaggtgg cgctgctcga gcgggccgac
     1321 cgccagcgcg cgaccggggc accgctgctg gtcgggctca ccgaggccga gcgcgccgat
     1381 ggcccactcg tcgcctggct gagtcgcgta cgcctggacg tgccggcgct ggccgaccga
     1441 accgcggacg tcattcccct cgcgcgtcac ttcctcgcgg aggccgcgcg ccgactcggc
     1501 aagaccatcc cgggcttcac gaccgacgcg gagcaccggc tgctcgcgca cgcgtggccc
     1561 ggtaacatcc gggagctgcg cgaggtcgtg gagcgcgcgg ccgccgccgg ggtcggcgcc
     1621 atccgcccgg agctgctcgt gctcggcggc accgcgaccg cgcccgcctg gttccccacg
     1681 ggcccgcccc ggccgctccg cgagatcgag gacgcctaca tcgaccacgt gatcgcgctg
     1741 tcggggggaa acaagacacg cgcggcccag ctgctcggca tcgcacgcga gaccctgcgc
     1801 acccgcatgc tcagccgtgg caacccgggg gtgtcggggg aggagcggag tgcctgacgc
     1861 catgaagatc ctcacggtgg acgactcgga cgtcatgcgc cggatcatca agcacgggtt
     1921 gaaggccatc ggctacgacg aggtgatcga agccgagaat ggtcaggttg ggctcgccag
     1981 gatccagagc gaggcggtgg acttcgtcat caccgattgc tccatgccgg tgatgtccgg
     2041 gctcgaaatg gtgacggcca tccggtcgaa tcccgccacc aaggacctgc cggtcctgat
     2101 gatcacggcc cccgaccggc aggaggagat cgtccaggcc gtccgcgcgg gcgtgagcgg
     2161 gtacatcctc aagcccttcg cggccgagac gctgcacgag aagatggagc aggtgctggg
     2221 ccgggcgtga gcacgctccg ccgctccgag ggtcaccggc ggacgcgggc tccccgcggg
     2281 atcgtcacca cgacgaccgt cgcccgcgag atgcgcgacc tgctcggcgg catcgagatg
     2341 tgcgcgacgc tgatcgccag gcactgcgcg ggcgaggaca ccgtggcgcc gctcaccggc
     2401 cgcctgctgg gcggcgtggc caggctgcgc gaagcctccg ccaacctcct gtcgctcggt
     2461 agccggtccg tcgccccggc cggcccggcg cccgacgacg aggcgccggg cgctcgcgtg
     2521 aagaccgtcc ggcgccgagc gcgcgtcgcc gggcgccgcg cgcgcgtcgt gaccgtcacg
     2581 tccggcaagg gcggtgtcgg caagaccacg ctcgtggcca atctggggct cgagctggcc
     2641 cgactcggcc ggcgcgtgct gctgctcgac ggcgcgctcg gactggccaa cctcgccatc
     2701 ctcttcaacc agacgcccaa gcgcacgctc gaggacgccc tggcccgccg ctgcagcgtg
     2761 gacgacgtcg tgctccaggt gcgcgagggt ctggggatca tcccgtccgc gcccggcgcg
     2821 acgcggctcg ccgagctcga gcccgccgag cgcgcgagcc tgctcggcga gatcgtccgg
     2881 ctcggcgccg aggccgacgt gctcctcatc gacaccggcg ccggcatctc cccgatcgtt
     2941 caggagatgg tcgggcttgc cgaccgcgcg ttcctcgtca ccacccacga gcccaccgct
     3001 ctgtccgacg cctacggcct gctcaaagcc gtgagcacgg gtccggcggg cggctcgccg
     3061 cgcatcgatg tcgtcgtgaa catggcgcgg agccacgccc acgcgctcga cacgcacggc
     3121 cgcctcggcc agctcaccga gcgcttcctc ggcttcacgc cggaactcgt cgcggtcgtc
     3181 ccgcgcgacg aggcggtggg cgaagcgatc gtgcgccagg agccgctcac tcgcatctac
     3241 ccgtatgcgc cggccacgcg cgccatcgtg gcgttcgcgc ggctgctcgc caacccacga
     3301 ggacccgacc atgccaacgc acacagcaca ccgctggccg ttccagtcag ccggtaagac
     3361 gatccgcccg gaggccgtcg aggccgcgtg gcaggagtac cgcgccaccc gctcggcgga
     3421 cgcgcgcgag acgctcgtgc agcgctacct ccatctcgtg cgctacatgg cggggcggct
     3481 ctcccgcacg ctacccccga gcgtcgagtt cgacgacctc gtgtcgtccg gcctggtagg
     3541 cttcctgagc gcgctcgaca gcttcgatcc ggaccacggc accgacttca gcgtctacgc
     3601 cctgacccgc atccgcggcg cgatggtcga cttcgtgcgc gagatcgacc ccatcggccg
     3661 cgtcacgcga cgcaagctgc gcgcggtcga gcgcacgctc gtccagctgg agcaggagct
     3721 gtcgcgcccg ccgtcggcca cggagaccgc cgcgcgcctg ggcatcacca cggacaccta
     3781 ccacgagctg atgagccagg cgacggcggc gatgacgctc tcgctcgagc gtatccagag
     3841 tgacggcgac gacgacggcg tgcccggcac gcgcgcctgg gtggccgaca cggcgaccaa
     3901 ggacccgctg accacgctcg ccgacaagga cgacgtgggg cgcctggctg gcctggtagg
     3961 caccctgtcg tcggcacagc agctcgtgct gcacctgcac tacgtcgagg agctgaactt
     4021 ccgtgaggtc gcgatggcga tggatatctc ggagtcccgc gccacgcagc tacacacggc
     4081 ggcggtgctg gcgctccgca gccaggcacg gggctgcgcg gtcgccagcg gacggaccgg
     4141 aggatgaggc tgcgacgctc ccgggtggca tcgacgtgac cgcctgacga cgcgacggca
     4201 ggagcccgtt tggggacgcc tgccgctcgt tcccgttgcg cttcccagcc cagacacagc
     4261 ggcgggcgcg gcccgcgacc ggtctgccgg tgtcagccgg ccccggggac cgcacccgcc
     4321 cggtggggtg ttcggcagat tccaccggtc tccggagacg tccgtggccg cccctgccgc
     4381 ccggtttccg ggtgacgcgg gcggctccgt caacccgcgc agccagcgcg ccagcagggc
     4441 taccgcttcg cgagcgggat ggggcgatgg agacccctac cggacggcag gtagctcgat
     4501 gaggaaggtg acgccgccgt acaagcggtt cgccgcggtg agacggcccc cgtgcgccgt
     4561 cacgatcgcg tgcgcggcgg cgaggccgac cgatggcccg cgcccttcgc gccgcgggac
     4621 gaacggatcg aagacatgag gcagcacgtc ggcggggatc gacggtccgt cgtcttcgac
     4681 cgctagcgtc acccacggtc cggccgaatc gccccgcgcc cccgtcgtga gccgtaccag
     4741 gccccggctg cgcaccgccg tctcggcctc gaggacgagc gccagcacgg cgcgctcgag
     4801 ctgcgcccca tcggccgcca ccgccggcaa ctgcccggcc agcacgcgct cgacgcgcat
     4861 gccgcgcgcg cgcatgcgcg gctcgcgcag atggagcagc cgctcgacga gcgcgtcgag
     4921 actgacgacc gcacggacgc cgcccccgct ccagaagtgg cggagctcgt tgagcaggtc
     4981 gccgatctgc cggcaatgag cgtcgagc
//
LOCUS       DATIAZ010000103         5673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1604058, whole genome shotgun sequence.
ACCESSION   DATIAZ010000103 DATIAZ010000000
VERSION     DATIAZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604058"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VLF19_03550"
     CDS             <1..1171
                     /locus_tag="VLF19_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSE92356.1"
                     /translation="ATASILLALYVVVARRQGVAPERLGGTTQNDILKEYIARGTYIF
                     PPGPSMRLVTDVFAYCRERMPRWNTISISGYHMREAGCTAVQEVAFTLANAVAYVEAA
                     REAGLAVDEFAPQLAFFFNAHNDLIEEVAKFRAARRLWARLVRDRFGARDPRATMLRF
                     HAQTAGSTLTAQQPENNIVRVTVQALAAVLGGCQSLHTNSMDEALALPTERAVRIALR
                     TQQILAHESGVAGTADPLGGAYAVERLTRDIEEAAEAYIRTIDGLGGAVRAVTFMQRE
                     IQDAAYRWQREVEDKARVMVGVNEFVTEDAPPANLFQVDTGVGEALAERVRRLRAARA
                     GDAAERALDALEHGARGATNLVPLIVDAVDASVTLGEICERLRAVFGTHQPEVTV"
     gene            1345..2136
                     /locus_tag="VLF19_03555"
     CDS             1345..2136
                     /locus_tag="VLF19_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92357.1"
                     /translation="MSGPPIILSVLLTAAPAAAGVADRIGATFALMAQDFIRAAQPIE
                     GVVVSAEGGLLYLDVGEAAGAQVGQELTVFRKGEPFYHQITGRLLGRFEDLLGYAQIR
                     RVEPGFSEAVFIALPDRPHPRAEDGVRISRGRIRLAVTPVLDLTTTSADVRRVPYMLA
                     SVLERSRRFQVIDPLTVGDMFAAGSVRVEEVLARPERAVRNAKNLDVSGWLIPVLLER
                     RGVLYLDVTWVSAVTGTPLFSRRRPLLPTGASEAQRFPWEPRPED"
     gene            2138..2863
                     /locus_tag="VLF19_03560"
     CDS             2138..2863
                     /locus_tag="VLF19_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92358.1"
                     /translation="MLWYRPMRTWLLALVVLLAVATGCNKVREIAGFAPQADPSVTLK
                     SAETRWLLIKNPRYGDVPSEPEYVWVEEDKIPTTLATLIRGKGAIIATPEIVAKYGAP
                     PGGGKISPRQGVPYQNTAEPPRAEARGGVAAAPGGGAATPVAAALSAPAPTRGFVVYV
                     DTTRIVVDLTSRDGVRAGSVVSLRRDRIQIVHPVTGEVLGELDDEVGTARITEMRDKF
                     SVAEIQSVAPGAKIQVKDRAVLK"
     gene            2873..3871
                     /locus_tag="VLF19_03565"
     CDS             2873..3871
                     /locus_tag="VLF19_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSE92359.1"
                     /translation="MPTPTLERVLKERVAALVGDDLRSVEAEIHHQLDSPVGLIQEMG
                     GYIAGAGGKRLRPMLLLLTASLAGCRGPRAVRLACVVELLHTATLIHDDVVDQAPLRR
                     GRPSANAQWGDDASVLVGDHLYSKSFAMLVHDNDRGVMETLARATVSMTEAEVLQLEC
                     KRAGITTETDYVRIITQKTASFISACCRIGGLLGALPPAQVDALTRYGLDIGVAFQIS
                     DDSLDFIADQERLGKAIGSDLREGKLTLPLIAMLQRAPRGDVEHVRTLLRRRALAPAE
                     LEAIRRLVVEHDGVDYARAQASARAEAAKASLGAFVPSEEREVLTLIADFVVDRDR"
     gene            4014..4829
                     /locus_tag="VLF19_03570"
     CDS             4014..4829
                     /locus_tag="VLF19_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSE92360.1"
                     /translation="MTTDFGSRDPYVAEMKGVILGLTRDVVLVDVTHEVEAQQIAEAA
                     LVLEGVVEAFPAQTIHLAVVDPGVGTSRRGLVVEAGGHLFVGPDNGLFTAVLARGGWQ
                     AFELTAAELRRPTVSRTFHGRDIFAPAAAHLALGVPPARFGAPVADPVRLPWPEARAV
                     AGGIAGAVIHVDRFGNLVTSIDATLVGSFAGGPAGPGTVRIAGRALPLVGTYADLPPH
                     AAGALVGSRNRLEVVVREGSAAAVLEARRGMPVLLSRTRLLPARTGRRRRSSQ"
     gene            complement(4771..5100)
                     /locus_tag="VLF19_03575"
     CDS             complement(4771..5100)
                     /locus_tag="VLF19_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSE92361.1"
                     /translation="MPTYEYQCDQCHRTFEVRQRITAEPLTRCEACGGTLKRLISPAP
                     FILKGEGWYVTDYPSEARKKAVESEKSSAGKSPSGEQSSTPAPKETPTASSSAAAPSS
                     PAAASSD"
     gene            5224..>5673
                     /locus_tag="VLF19_03580"
     CDS             5224..>5673
                     /locus_tag="VLF19_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE92362.1"
                     /translation="MRRLQARAAYERGLSHLEKREAAPALTAVQEAVSIDATVPVYWN
                     ALGWIYLQLGHPEVAYRAFSRAVELDGTWAEAQLNAGVALAEMGRWEEALAAYRKVLN
                     LPGVSMPHATYQNMGVAFYNLKRYREAEEALRFAIGLEPKLAAAYYHL"
BASE COUNT          787 a         1997 c         2089 g          800 t
ORIGIN      
        1 cgcgacggcg tcgatcctgc tcgcgctgta cgtcgtcgtc gcgcgccggc agggcgtggc
       61 ccctgagcgg ctcggcggca ccacgcagaa cgacatcctc aaggagtaca tcgcgcgggg
      121 gacgtacatc ttcccgcccg gcccctccat gcggctggtg accgacgtgt tcgcctattg
      181 ccgcgagcgg atgccgcgat ggaacacgat ctcgatctcc ggctatcaca tgcgcgaggc
      241 gggctgcacg gcggtgcagg aggtggcctt cacgctcgcc aacgccgtcg cctacgtgga
      301 ggcggcgcgc gaggccgggc tggccgtgga cgagttcgcg ccccagctcg cgttcttctt
      361 caacgcccac aacgatctga tcgaggaggt cgcaaagttc cgcgccgcgc gccggctgtg
      421 ggcgcgcctc gtgcgcgacc gcttcggcgc ccgcgacccc cgcgcgacga tgctgcgctt
      481 ccacgcgcag acggcgggca gcacgctgac cgcgcagcag cccgagaaca acatcgtgcg
      541 ggtgaccgtg caggcgctcg ccgccgtgct cggcggctgc cagtccctgc acaccaactc
      601 gatggacgag gcgctggcgc tgcccaccga gcgcgccgtc cgcatcgcgc tgcgcacgca
      661 gcagatcctc gcccacgagt cgggcgttgc cggcaccgcg gacccgctgg gcggggccta
      721 tgccgtcgag cgcctcaccc gtgacatcga ggaggccgcg gaagcgtaca ttcggacgat
      781 cgacggcctc ggcggcgccg tccgcgcggt cacgttcatg cagcgcgaga tccaggacgc
      841 ggcgtaccgc tggcagcgcg aggtcgagga caaggcgcgg gtaatggtcg gcgtcaacga
      901 gttcgtcacc gaggacgcgc cgcccgccaa cctcttccag gtcgacaccg gcgtcggcga
      961 ggcactggcc gagcgcgtgc gtcggctgcg ggccgcccgc gcgggggacg ccgccgagcg
     1021 cgcgctcgac gcgctcgagc acggcgcccg cggcgcgacc aatctcgtcc cgctgatcgt
     1081 cgacgcggtg gacgcctccg tgaccctcgg cgagatctgc gagcggctcc gcgccgtgtt
     1141 cggcacccac cagcccgagg tgacggtgtg acgcgttcat tgttgcgggc catactgcgg
     1201 cgcgaggcgc ttcgaggcgt gccacggcta cgccgttgcc tgacgagcgt tcggatatgg
     1261 gggcccatgt cggggcgggg cgggaatcga ggcgtgccac ggctacgccg gggcatgccg
     1321 agcgtttggg gggcatgggg ggccatgtcg gggcccccca ttatattgtc cgtcctgctc
     1381 acggccgcac ccgcggcggc gggtgttgcc gaccgcatcg gcgccacgtt cgccctgatg
     1441 gcgcaggact tcatcagggc cgcgcagccg atcgagggcg tcgtggtctc cgccgagggc
     1501 ggtctcctct acctcgacgt gggtgaggcc gcgggcgcgc aggtcggcca ggagctgacg
     1561 gtcttccgga agggcgagcc gttctaccat cagatcaccg ggcggctgct cggccggttc
     1621 gaggacctgc tggggtacgc gcagatccgg cgtgtggagc cggggttctc ggaggcggtc
     1681 ttcatcgcgc tgcccgaccg cccgcatccc cgcgccgagg acggcgtgcg catcagccgc
     1741 gggcggatcc ggctcgccgt gacgccggtg ctcgacctca ccacgaccag cgccgacgtc
     1801 cgccgcgtcc cctacatgct cgcgagcgtg ctggagcgct cccggcgctt ccaggtcatc
     1861 gatccgctga ccgtgggcga catgttcgcc gccggttccg tgcgcgtgga agaggtgctg
     1921 gcgcgccccg agcgcgccgt gcgcaacgca aagaacctcg acgtgtcggg ctggctcatc
     1981 cccgtgctgc tcgagcgccg gggcgtgctc tacctcgacg tgacgtgggt gtcggccgtg
     2041 acggggacgc ccctgttctc gcgccgccgt ccgctgctgc cgacgggcgc cagcgaggcc
     2101 cagcgcttcc cctgggagcc ccggccggag gactgacgtg ttatggtatc gccccatgag
     2161 gacgtggctg ctcgccctcg tcgtcctgct cgcagtcgcg accggatgta acaaggtacg
     2221 ggagatcgcg gggttcgcgc cccaggccga tccctcggtc acgctgaagt cggccgagac
     2281 gcgctggctc ctcatcaaga acccgcgcta cggcgacgtg ccgagcgagc cggaatacgt
     2341 ctgggtcgag gaggacaaga tccccacgac gctcgccacg ctgatccgcg gcaagggcgc
     2401 catcatcgcg acgccggaga tcgtggcgaa gtacggggcg ccgcccggcg gcggcaagat
     2461 cagcccgcgc cagggcgtgc cgtatcagaa cacggccgaa ccgccgcgcg cggaggcgcg
     2521 cggcggggtg gcggcggcgc cgggaggagg tgcggcgacg cccgtcgccg ccgcgctctc
     2581 ggcgcccgcg cccacgcgcg gcttcgtcgt ctacgtggac accacgcgga tcgtcgtcga
     2641 tctgacgtcg cgcgacgggg tgcgcgccgg gagcgtggtg agcctgcgcc gcgatcgcat
     2701 ccagatcgtg caccccgtca ccggggaggt gctgggcgag ctcgacgacg aggtcggcac
     2761 cgcccgcatc accgagatgc gcgacaagtt ctcggtcgcg gagatccagt ccgtcgcccc
     2821 gggcgccaag atccaggtca aagaccgcgc cgtcctcaag tagcgttccc gcgtgcccac
     2881 cccgacgctc gagcgggtcc tcaaggagcg cgtggccgcg ctcgtcggcg acgacctccg
     2941 gtccgtggag gccgagatcc accaccagct cgactcgccg gtgggcctca tccaggagat
     3001 gggcggctac atcgccggcg ccggcggcaa gcggctgcgg ccgatgctgc tgctgctgac
     3061 cgccagcctg gccggctgcc gtggcccgcg tgcggtccgg ctggcgtgcg tggtcgagct
     3121 gctccacacg gcgacgctta tccacgacga cgtggtcgac caggctccgc tccggcgggg
     3181 ccggccgtcg gcgaacgccc agtggggcga cgacgcctcg gtgctcgtcg gcgaccacct
     3241 gtactcgaag tcgttcgcga tgctcgtcca cgacaacgac cgcggggtga tggagacgct
     3301 ggcgcgggcg accgtctcca tgaccgaggc cgaggtgctc cagctcgagt gcaagcgcgc
     3361 gggcatcacg accgagacgg actacgtgcg catcatcacc cagaagacgg cgtcgttcat
     3421 ctcggcctgc tgccgcatcg gcgggctgct gggcgcgctg ccgcccgccc aggtggacgc
     3481 gctgacgcgc tacgggctgg acatcggcgt ggccttccag atctccgatg actcgctcga
     3541 cttcatcgcc gaccaggagc gcctgggcaa ggccatcggc agcgacctcc gggaaggcaa
     3601 gctgacgcta cccctgatcg cgatgctgca gcgggcgccg cgcggcgacg tggagcacgt
     3661 gcggacgctg ctccggcgcc gcgccctggc gccggcagag ctggaggcga tccgccggct
     3721 ggtcgtcgag cacgacggcg tggactatgc tcgcgcccag gcctccgcgc gtgcggaggc
     3781 cgccaaggcc agcctgggcg ccttcgtgcc ctcggaggag cgcgaggtcc tgaccctgat
     3841 cgcggacttc gtcgtcgatc gcgatcgata aggtccccgg cgggggcgaa gccccctccg
     3901 gtagctcccc ccaaggacag gatggcggcg gcaaagccgc cgctcgaacc agaggtaccg
     3961 ccggaatggg ccggcccccg agcggagaca cgacggcgag agccatcatc acgttgacga
     4021 cggatttcgg atcgcgcgac ccctacgtcg ccgagatgaa gggcgtcatc ctcggcctca
     4081 cgcgcgacgt ggtgcttgtg gacgtgacgc acgaggtgga ggcgcagcag atcgccgagg
     4141 ccgcgctcgt gctcgagggg gtcgtcgagg cgttcccggc gcagaccata catctggccg
     4201 tggtggatcc gggggtgggc acgagccggc gggggctcgt cgtcgaggcg ggcggccacc
     4261 tgttcgtcgg ccccgacaac gggctgttca ccgcggtgct ggcccggggc ggctggcagg
     4321 ccttcgagct gacggcggcc gagttgcggc ggccgaccgt gagccgcacg ttccacgggc
     4381 gcgacatctt cgctcccgcc gccgcgcacc tggccctggg cgtcccgccg gcgcggttcg
     4441 gcgcaccggt ggccgacccg gtgcggctgc cgtggccgga ggcgcgggcg gtggccggtg
     4501 gaatcgccgg tgccgtcatc cacgtcgatc gcttcggcaa cctcgtcaca tcgatcgacg
     4561 ccaccctggt gggcagcttc gcgggaggac cggccggccc cggaacggtc cggatcgccg
     4621 gccgggcgct gccgctcgtc gggacctacg ccgacctgcc gccgcacgcc gccggagcgc
     4681 tggtcggctc gcggaaccgt ctcgaggtgg tcgtccgcga ggggagcgcg gccgcggtgc
     4741 tcgaggcccg gcgcgggatg ccggtgctgc tcagtcggac gaggctgctg ccggcgagga
     4801 cggggcggcg gcggaggagc tcgcagtagg agtctccttc ggcgccgggg tgctagactg
     4861 ctcgccggat ggagactttc ccgcgctgct cttctcggat tccacggctt tcttgcgagc
     4921 ctccgaggga tagtcggtga cgtaccagcc ctcgcccttg aggatgaagg gagcgggcga
     4981 gatcagccgc ttgagcgtcc cgccgcaggc ctcgcagcga gtgagcggct cggcggtgat
     5041 gcgctgccgg acctcgaacg tccgatgaca ctggtcacac tggtactcgt acgtcggcac
     5101 aagatcgaac gctacatggg agggtgagga gtgtcaagac aacgagcgct ccaggcaacc
     5161 gcgctcgcga tggcgctcgc gcttggcggc tgtgcctcca cgagctcgga gaaagaggcg
     5221 gaggtgcgcc ggctgcaggc gcgcgcggcg tacgagcgag ggctctccca cctcgaaaag
     5281 cgcgaggctg cgcccgcgct gacggccgtc caggaggcgg tgagcatcga cgcgacggtg
     5341 cccgtctact ggaacgcgct cgggtggatc tacctgcagc tcggccaccc cgaggtggcc
     5401 taccgtgcgt tctcccgggc ggtcgagctg gacggcacgt gggccgaggc ccagctcaac
     5461 gcgggggtcg cgctggccga gatgggccga tgggaggagg cgctggcggc gtaccggaag
     5521 gtcctcaacc tcccaggagt gtcgatgccg cacgccacct accagaacat gggggtcgcc
     5581 ttctacaacc tgaagcgcta ccgcgaggcc gaggaagccc tgaggttcgc catcgggctg
     5641 gagccaaagc tggcggccgc ctactaccat ctc
//
LOCUS       DATIAZ010000104         8096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1473009, whole genome shotgun sequence.
ACCESSION   DATIAZ010000104 DATIAZ010000000
VERSION     DATIAZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473009"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VLF19_03585"
     CDS             <1..390
                     /locus_tag="VLF19_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE92363.1"
                     /translation="TMKHKVRLKARVYPGFVDVAAARRARTFAEYDRAVTAPLNGFAD
                     EMDYWTRASSRPYLSRIRVATRIINALDDPFVPADALPEPARLPACVSLVLTPRGGHV
                     GFVDSARPGRASWAERSAVAFLATQMT"
     gene            387..704
                     /locus_tag="VLF19_03590"
     CDS             387..704
                     /locus_tag="VLF19_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92364.1"
                     /translation="MTTDRHGLSVTTASTPALEAYDRGAEGLLGWDGATLDHFGAAVA
                     HDPALAVAHAGAGVCLFLEERFEEAREAAATARRHAAEGQTERERSHVEAMAHWRTRR
                     RRA"
     gene            complement(874..2301)
                     /locus_tag="VLF19_03595"
     CDS             complement(874..2301)
                     /locus_tag="VLF19_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSE92365.1"
                     /translation="MNSVDLCFTPAVRLAALIRRRTVSPLEVTRAVLARIERVNPRLG
                     AYCTVAADHALAAARAATAAVTRRSARLGPLHGVPVSIKDLTPTKGIRTTWGSRLFED
                     HVPDEDGLIVERLRAAGAVVLGKTNTPEFGAGANTRNDVFGFTRNPWNPALTCGGSTG
                     GGAVALATGMGPLAQGSDLGGSLRIPASFCGVVGFRPSPGLVPIHPTPQAWNTYSVQG
                     PMARTVADVALMLAAIAGPDPRSPISYPVDARAFLAAVRRPSARGLRVAWGGDLGVTP
                     VDSEVLAIARGASGVFRHLGARLSVARPDFDGLRDIVITSRGLSMVAAHGDKLPKWRD
                     VMQPNLVKNIEQGFALTAADAARCEASRTALYHRVRDFFANHDLLLTPTTAVKPFPVE
                     QWYPTEINGKPMPTYVDWVLLTYAFTVVGLPAISVPCGFTADTLPVGLQIVGRWRDEA
                     TVLRAAAAFEAAAPWAGRRPPDPDR"
     gene            complement(2429..3367)
                     /locus_tag="VLF19_03600"
     CDS             complement(2429..3367)
                     /locus_tag="VLF19_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92366.1"
                     /translation="MDGSTHAFPIAEPLAVERPASSLASEAWRRFRRHRLALFGACVL
                     AAIALVVYAGPLAYRTPIDEIDFRAKLERPSVAHPLGTDDLGQDLLARVLYGGRISLA
                     VGVVAMLIALTLGVVIGAVSGYYGRWLDQALMRITDLFLSLPQLPLLLLVVYLFRDFV
                     RSWLGPEAGIFVLIVAVIGALRWMPVARLVRAQFLSLREQDFVQAARGLGAPPRRQIV
                     RHILPNAFGPVIVAASLDVAAAIIAESTLSFLGLGFPPDIPTWGRILFDAKDNLDHAP
                     HWALFPGTAIFLTVLSINYIGDGLRDALDPRRVLTH"
     gene            complement(3333..4292)
                     /locus_tag="VLF19_03605"
     CDS             complement(3333..4292)
                     /locus_tag="VLF19_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92367.1"
                     /translation="MSRYVLRRLLIAIPALLGISVVLFSVLALAPGDPFEELATNANV
                     PPEVAQRLRVQFGLDDPLPLRYVRWLTSMVRGDWGFSFQSRMNVEDLILQRLPVSLFV
                     LGSAYVLALLVALPVGILSAVRPYSIFDQLATTLAFIGFSLPTFFTGLLFILVFSIWL
                     DWLPFIYRADVDSTGLRFFLDHARQAVMPVCVLGLFQGAVLTRFVRASVLDVVRLDYV
                     NTARAKGLSETRTIGKHVVRNALIPVVTVIALQVPTIFAGAVVTEQIFRVPGIGSLLI
                     AAILASDTPVVMAITFVFAVLVVLFNLVADIVYGWLDPRISYS"
     gene            complement(4342..6141)
                     /locus_tag="VLF19_03610"
     CDS             complement(4342..6141)
                     /locus_tag="VLF19_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HSE92368.1"
                     /translation="MDEREIRSLLHEVKTGRLSRRSFVQTMLALGLTGPMVQQMFASV
                     GIARAQTRPAFTPSKRGGGGRLRLLWWQAPTLLNGHFATGTKDQDASRVVTEPLVSFD
                     PDGNFLPILAAEIPTIQNGGLARDHKTVTWRLKKGVQWHDGRPFTADDVIFTWEYVAD
                     PATASVNVTAYRDIDGIDRVDDHTVKVHFKAPAAFWYDAFFGVRGYILPRHLLKDFKG
                     AKSREAPYNLKPIGTGPYRIVDFKPGDSVRYEINPNYHVPNRPFFDVVELKGGGDAPS
                     AARAVFQTGEFDYAWNLQVEWEVLRRLKESAKARLINSPTGNIEHVQLNQTDPWTEVE
                     GERASVKSRHPVFQDPKLRQAMALLVDRGTVHEELYGETGQATANFLNAPSKFRSPNM
                     KWEFNVDKASKLLDEAGYKRGGDGFRVGKDGKRIKLLFQTSTNSLRQKNQAIIKQACA
                     KAGLDVELKSVVASVFFSSDPANPDTYAHFFADMQMYNTTQTSPDPQFFMNQFLSTEA
                     AQKSNKYAGRNPTRWQNAEYDKLWKAAEVEFDPVKRAALFVRMNDLVVSNVVVIPEVW
                     RNRSSAAVTKLQGLDLSGWDSTLANLANWYREA"
     gene            complement(6151..7179)
                     /gene="pdxA"
                     /locus_tag="VLF19_03615"
     CDS             complement(6151..7179)
                     /gene="pdxA"
                     /locus_tag="VLF19_03615"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSE92369.1"
                     /translation="MRRRPVLGVTMGDPAGVGPEIVARACAEPGVREASAPVVIGSAA
                     VMREAVALVGAPLTVHAVGAVADCAFRPATLECLDVDNVNAAALPRGEVSAEAGRAAY
                     EYVERAVALARAGEIDGIVTAPINKEALAAAGMPHTGHTEILAQLSGTKDFAMLLMGH
                     DLRVIHVTTHVALRRVPDLVTRGRVLQVIRLAQKTMEGLGRPAARIAVAGLNPHAGED
                     GLFGDEEQRAIVPAIEAARAEGLTVIGPLPADTLFSRARGGEFDIVVAMYHDQGHIPV
                     KTVGFTYDDARKTWTGLSGVNVTVGLPFLRVSVDHGTAFDRAWKGIANHESMVEALDV
                     AVRMLEKR"
     gene            complement(7176..>8096)
                     /locus_tag="VLF19_03620"
     CDS             complement(7176..>8096)
                     /locus_tag="VLF19_03620"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HSE92370.1"
                     /translation="RVAAAAAAAPARRYFKKIDSTLRGNLGAEVDAAMRATGAAGAVL
                     TPAFPAQGRVVIDRILFVEGRPVAETALGRDPERPRRDSSNVVDRVRPDLDRPLAWIP
                     IDQVRAGPDALNARLRRLRGTVAVADAETDEDLAALVDAALVLDPAPLLIGAAGLAHA
                     LAARLGLLPERAPLPSGSRWLVVAGSRHPVTRAQIAAARAAGMRVVASAEAEAPGRAQ
                     VAARLAAEARRLLEAEAFDVVAVTGGQTAVALYRALEAERIDLVGAPVPGLALGYMRA
                     PRRPEQALLTKAGGFGAPDLFVSLWRRAAA"
BASE COUNT         1207 a         2761 c         3038 g         1090 t
ORIGIN      
        1 acgatgaagc acaaggtgcg gctcaaggcc cgcgtgtacc ccgggttcgt cgacgtggcg
       61 gcggcccggc gcgcccgcac gttcgccgag tacgaccgcg cggtcaccgc gccgctgaac
      121 ggcttcgccg acgagatgga ctactggacg cgcgcgagca gccggccgta cctctcgcgc
      181 atccgcgtcg cgacgcgaat catcaacgcg ctcgacgacc cgttcgtgcc ggccgacgcg
      241 ctgcccgaac cggcgaggct gccggcctgc gtctcgctcg tgctgacccc gcgcggcggt
      301 cacgtgggtt tcgtggactc ggcgcgcccc ggccgcgcct cgtgggccga gcggagcgcc
      361 gtcgccttcc tcgccacgca gatgacatga cgaccgaccg tcacggcctg tccgtcacca
      421 ccgcctcgac ccccgcgctc gaggcctacg accgcggcgc cgagggcctc ctcggctggg
      481 acggcgccac gctcgatcac ttcggcgcgg cggtcgccca cgacccggcg ctggccgtcg
      541 cccacgccgg cgccggcgtc tgcctgttcc tcgaggagcg gttcgaggag gcgcgggagg
      601 ccgccgcgac cgcgcgccgc cacgcggccg agggacagac cgagcgggag cggtcgcacg
      661 tcgaggcgat ggcccactgg cgcactcgtc gtcggagggc gtgaggcggc ggatccagac
      721 tcaccgtccg gcgggccgga gccgacccct tgatgcgttg cgcggagtcg gccgtcgcat
      781 cggtccatcg acgcgctccg gcgtgtgacg ggcgtccccc ctgggctccg cagcctggct
      841 gtataagtgg cccaggggag ttcaagcggc tggctagcga tccgggtcgg gggggcggcg
      901 gcccgcccag ggcgcggcgg cctcgaaggc ggcggccgcc cgcagcaccg tggcttcgtc
      961 gcgccagcgg ccgacgatct gcagtccgac gggcagggtg tcggcggtga agccgcaagg
     1021 gacggagatc gcggggagtc cgaccacggt gaacgcgtat gtgagaagga cccagtcgac
     1081 ataggtcggc atcggcttgc cgttgatctc cgtcgggtac cactgctcga ccgggaaggg
     1141 cttcaccgcg gtcgtgggcg tcagcagcag gtcatggttc gcgaagaagt cacggacgcg
     1201 gtggtagagc gccgtgcgcg acgcctcgca ccgggcggcg tcggccgccg tcagcgcgaa
     1261 gccctgctcg atgttcttca cgaggttcgg ctgcatcacg tcgcgccatt tcggtagctt
     1321 gtcaccgtgg gccgcgacca tgctcaggcc ccggctggtg atgacgatgt ccctcaggcc
     1381 gtcgaagtcc ggcctggcca cgctcagccg ggcgccgagg tgccggaaga cgccactggc
     1441 ccctcgagcg atggcgagga cctcgctgtc caccggcgtg acgccgaggt cgccgcccca
     1501 ggccacgcgc aggcctcgcg cggacggtcg ccgcacggcg gcgaggaagg cgcgcgcatc
     1561 gacggggtac gagatgggcg agcgtgggtc gggccccgcg atggccgcga gcatcagcgc
     1621 gacgtcggcg accgtgcgtg ccatcggccc ctgcacgctg taggtattcc acgcctgcgg
     1681 cgtcggatgg atcggcacga gcccgggcga cggccggaat cccaccacgc cgcagaagga
     1741 ggcggggatg cgcagcgagc cgccgagatc ggagccctgc gcgagagggc ccatccccgt
     1801 ggcgagagcg acggcgccgc caccagtgga gccgccgcac gtcagggccg ggttccaggg
     1861 attgcgcgtg aacccgaaga cgtcgttgcg ggtgttggcg ccagcgccga actcgggcgt
     1921 gttggtcttg ccgagcacga cggcgccggc cgcgcggagg cgctcgacga tgagcccgtc
     1981 ctcgtcgggc acgtggtcct cgaagaggcg cgacccccac gtcgtgcgga tgcccttcgt
     2041 gggcgtgagg tccttgatcg agaccggcac gccgtgcagc ggcccgagcc gcgcgctgcg
     2101 ccgggtgacg gcggcggtgg ctgcccgcgc ggcggccagc gcgtggtcgg cggccacggt
     2161 gcagtaggca ccgagacgcg ggttcacccg ctcgatgcgc gccagcacgg cgcgggtcac
     2221 ctcgagcggc gagacggtgc ggcgccggat cagcgcggcg agccgcacgg ccggggtgaa
     2281 gcagaggtcg acggagttca tgtccgtgac tatagcgttg gtcgcacctc tcgggtatcg
     2341 aggccgcgga gtctcgaggc ggccacggcg tggccgggca tgccgagcgg atgggggcct
     2401 gggggccatg tcggggcccc catgtccatc aatgcgtgag gaccctccgc gggtccagcg
     2461 cgtcgcgaag gccgtcgccg atgtagttga tggagagcac ggtgaggaag atcgcggtgc
     2521 ccgggaacaa ggcccagtgc ggcgcgtggt cgaggttgtc cttggcgtcg aagaggatgc
     2581 gcccccacgt cgggatgtcg ggcgggaaac cgaggccgag gaacgacagc gtcgactcgg
     2641 cgatgatcgc ggccgccacg tcgagcgagg cagccacgat gacgggcccg aacgcgttgg
     2701 gcaggatgtg gcggacgatc tggcgccgcg gtggggcgcc cagcccgcgc gccgcctgca
     2761 cgaagtcctg ctcccgcagg ctgaggaact gcgcccgcac cagccgcgcc accggcatcc
     2821 agcgcagcgc cccgatcacg gccacgatca ggacgaagat gccggcctcc gggcccagcc
     2881 agctccgcac gaagtcccgg aacaggtaga ccaccagcag cagcagcggc agctgcggca
     2941 gcgagaggaa caggtcggtg atccgcatca gcgcctgatc gagccagcgg ccgtagtagc
     3001 cggacaccgc gccgatcacg acgccgaggg tgagcgcgat cagcatggcc accacgccca
     3061 cggccaggga gatccgaccg ccatagagca cccgcgcgag caggtcctgg cccaggtcgt
     3121 cggtgcccag tgggtgggcg acgctgggtc gctcgagctt ggcccggaag tcgatctcgt
     3181 cgatcggcgt ccggtaggcg aggggccccg cgtacacgac gagggcgatg gccgccagca
     3241 cgcaggcgcc gaagagcgcc agacggtggc gccggaacct ccgccaggcc tcggaggcga
     3301 gcgacgaggc tgggcgctcg acggcgagcg gctcagctat aggaaatgcg tgggtcgagc
     3361 catccataga cgatgtcggc gacgaggttg aagaggacga cgagcaccgc gaagacgaag
     3421 gtgatggcca tcaccaccgg ggtatcggag gcgaggatgg ccgcgatcag cagcgatccg
     3481 atccccggca cgcggaagat ctgctcggtg acgacggcgc cggcgaagat cgtcgggacc
     3541 tgcagggcga tcacggtgac caccgggatc agcgcgttgc gcaccacgtg cttgccgatc
     3601 gtgcgcgtct cggagagacc cttggcgcgc gcggtgttca cgtagtcgag gcgcaccacg
     3661 tcgaggaccg aggcccgcac gaagcgggtg aggacggcgc cctggaagag gcccaggacg
     3721 cacacgggca tgacggcctg gcgggcatgg tcgaggaaga accgcagccc ggtggagtcg
     3781 acgtcggcgc ggtagatgaa cggcagccag tcgagccaga tgctgaagac gaggatgaac
     3841 aggagcccgg tgaagaaggt cgggagcgag aagccgatga aggccagcgt ggtggcgagc
     3901 tggtcgaaga tggagtacgg ccgcacggcc gagaggatcc ccaccgggag ggcgacgagc
     3961 agcgcgagca cgtaggccga gccgagcacg aagaggctca cgggcagccg ctgcaggatc
     4021 aggtcctcca cgttcatgcg gctctggaac gagaagcccc agtccccccg gaccatcgac
     4081 gtgagccagc gcacgtagcg caggggcagg ggatcgtcga ggccgaattg cacgcggagc
     4141 cgctgggcca cctcgggcgg gacgttggcg ttggtcgcca gctcctcgaa gggatcgccg
     4201 ggcgcgagcg ccagcaccga gaagagcacg acggagatgc cgagcagggc cgggatcgcg
     4261 atcagcagcc gacggaggac gtacctggac attggagggc ccgcggcggg gccgtcccgc
     4321 cccgccgcgg gccgcgtcgt cctacgcttc gcggtaccag ttcgccaggt tggccagcgt
     4381 cgagtcccag cccgacagat ccagcccctg gagcttggtg acggccgcgg aggagcggtt
     4441 gcgccagacc tccgggatga cgaccacgtt cgagaccacg aggtcgttca tcctgacgaa
     4501 cagcgccgcg cgcttgaccg ggtcgaactc cacctccgcc gccttccaga gcttgtcgta
     4561 ctcggcgttc tgccagcggg tcgggttgcg ccccgcgtac ttgttggact tctgcgccgc
     4621 ctcggtcgag aggaactggt tcatgaagaa ctgcgggtcg ggggaggtct gcgtggtgtt
     4681 gtacatctgc atgtcggcga agaagtgggc gtaggtatcg gggttggcag ggtccgagga
     4741 gaagaacacc gaggccacga cggacttcag ctcgacgtcg aggccggcct tggcacaggc
     4801 ctgcttgatg atggcctggt tcttctggcg cagcgagttc gtcgacgtct ggaacagcag
     4861 cttgatccgc ttgccgtcct tgccgacccg gaagccgtcg ccgccgcgct tgtaccccgc
     4921 ctcgtcgagc agcttgctcg ccttgtcgac gttgaactcc cacttcatgt tcggcgagcg
     4981 gaacttcgac ggggcgttca ggaagttcgc cgtggcctgc cccgtctcgc cgtacagctc
     5041 ctcgtggacg gtgccgcggt cgaccaggag cgccatcgcc tggcggagct tcgggtcctg
     5101 gaacaccgga tgcctcgatt tcaccgaggc gcgctcgcct tcgacctccg tccacggatc
     5161 ggtctggttg agctggacgt gctcgatgtt gcccgtcgga ctgttgatga gccgggcctt
     5221 ggcgctctcc ttgaggcggc ggagcacttc ccactccacc tggaggttcc aggcgtagtc
     5281 gaactcaccc gtctggaaca cggcgcgggc cgccgagggc gcatcgccgc cgcccttcag
     5341 ctccacgacg tcgaagaagg gacggttggg cacgtggtag ttcgggttga tctcgtagcg
     5401 gacggagtcg ccgggcttga agtcgacgat ccggtacggg cccgtgccga tcggcttgag
     5461 gttgtacggc gcctcgcggg acttggcgcc cttgaagtcc ttcagcaggt gcctgggcag
     5521 gatgtagccg cggacgccga agaaggcgtc gtaccagaac gccgccggcg ccttgaagtg
     5581 gaccttcacc gtgtggtcgt cgacgcggtc gatcccgtcg atgtcccggt aggcggtgac
     5641 gttgacggag gccgtcgccg gatcggcgac gtactcccag gtgaagatca cgtcgtcggc
     5701 ggtgaagggc cgaccgtcgt gccactgcac gcccttcttc agccgccacg tgaccgtctt
     5761 gtggtcgcgg gccaggccgc cgttctggat ggtcgggatc tcggcggcca ggatcggaag
     5821 gaagttgccg tcggggtcga acgacacgag gggctcggtc acgacgcgcg aggcgtcctg
     5881 gtccttggtc ccggtggcga agtggccgtt gagcagcgtg ggggcctgcc accacagcag
     5941 ccgcagccgg ccaccgccgc cccgcttgct cggggtgaac gccggccgcg tctgggcccg
     6001 ggcgatgccc accgacgcga acatctgctg caccatcggc ccggtcagtc cgagtgcgag
     6061 catggtctgc acgaacgacc ggcgcgacag gcggccggtc ttcacctcgt ggagcaggct
     6121 tcggatctct cgctcgtcca tggatccctc ctagcgcttc tcgagcatcc gcacggcgac
     6181 gtcgagcgcc tcgaccatgc tctcgtggtt ggcgatgccc ttccaggcgc ggtcgaaggc
     6241 cgtgccgtga tccaccgaca ccctgaggaa cggcaggccc acggtgacgt tgacgccgct
     6301 gagccccgtc caggtctttc gcgcgtcgtc gtaggtgaat ccgacggtct tgaccgggat
     6361 gtggccctga tcgtgataca tcgcgacgac gatgtcgaac tcgcccccgc gggcccgcga
     6421 gaagagggtg tcggcgggca gggggccgat caccgtcagg ccctcggcgc gtgcggcctc
     6481 gatggccggc acgatcgcgc gctgctcctc gtcgccgaag agaccgtcct cgcccgcgtg
     6541 gggattgagt ccggccaccg cgatccgcgc cgcgggccgc ccgagcccct ccatggtctt
     6601 ctgcgccagg cggatcactt gcagcacgcg cccgcgcgta acgaggtcgg gcacccggcg
     6661 gagcgcgacg tgggtggtga cgtggatcac ccggagatcg tggcccatca gcagcatggc
     6721 gaagtccttc gtgcccgaga gctgcgccag gatctccgtg tggccggtgt ggggcatgcc
     6781 cgccgccgcc agcgcctcct tgttgatggg cgccgtcacg atgccgtcga tctcgccggc
     6841 ccgcgccagc gccaccgcgc gctcgacgta ctcgtaggcg gcgcggccgg cctcggcgct
     6901 gacctcaccg cgcggcagcg cggcggcgtt cacgttgtcg acgtcgaggc actccagcgt
     6961 ggccgggcga aaggcgcagt cggccacggc gccgacggcg tgcacggtga gtggggcgcc
     7021 cacgagggcg acggcctcgc gcatcacggc ggccgagccg atgacgaccg gagcgctcgc
     7081 ctcgcgcacg ccgggctcgg cgcacgcgcg cgcgacgatc tccggtccga cgccggcggg
     7141 gtcgcccatc gtcacgccca ggaccggccg gcgtctcacg cggccgccct ccgccacagc
     7201 gacacgaaga gatcgggcgc gccgaaaccg ccggccttgg tcaggagcgc ctgctctgga
     7261 cggcgtggcg cgcgcatgta tccgagtgcc agacccggga ccggcgctcc gacgaggtcg
     7321 atgcgctcgg cttcgagagc gcggtagagt gccaccgccg tctgcccgcc cgtgaccgcg
     7381 acgacgtcga aggcttccgc ctcgagcagg cggcgcgcct cggcggccag acgcgcggca
     7441 acctgcgcgc ggcccggcgc ctcggcctcg gcactcgcga cgacgcgcat gcccgcggcc
     7501 cgagccgcgg cgatctgagc gcgcgtgacg gggtggcggc tgcccgcgac cacgagccag
     7561 cgggagccgc tcggcagcgg cgcgcgctcg ggaagcaggc ccaggcgcgc ggccagagcg
     7621 tgcgcgaggc cggcggcgcc gatgaggagc ggggcggggt cgagcacgag cgcggcgtcg
     7681 acgagggcgg cgaggtcctc gtccgtctcg gcgtcggcca cggcaacggt gccgcggaga
     7741 cgccggagcc gcgcgttgag cgcgtccggg cccgcgcgaa cctggtcgat gggaatccag
     7801 gcgagcgggc ggtcgaggtc gggccgcacg cgatcgacga cgttggagga gtcccgccgg
     7861 gggcgctccg ggtcgcggcc cagcgccgtc tcggcgacgg gccggccctc cacgaacagg
     7921 atgcggtcga tcacgacgcg cccctgcgcg ggaaaggcgg gcgtgaggac ggcgcccgcc
     7981 gcaccggtgg cgcgcatggc cgcgtcgacc tcggcgccga ggttgccgcg cagcgtggag
     8041 tcgatcttct tgaagtaccg gcgggccggc gccgccgcag ccgcggcggc cacccg
//
LOCUS       DATIAZ010000105         7557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1737231, whole genome shotgun sequence.
ACCESSION   DATIAZ010000105 DATIAZ010000000
VERSION     DATIAZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737231"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VLF19_03625"
     CDS             <1..295
                     /locus_tag="VLF19_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSE92371.1"
                     /translation="YTGSAGWMYRAGLESLLGFRLRGAVLHLDPCIPRAWRRFDITFR
                     YHASRYVITVENPSGVTRGISTIEIDGTALPPGPARLALMDDVATHRVRVVLG"
     gene            353..1987
                     /gene="pruA"
                     /locus_tag="VLF19_03630"
     CDS             353..1987
                     /gene="pruA"
                     /locus_tag="VLF19_03630"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HSE92372.1"
                     /translation="MTNTILSVPEPRNEPVLSYAPGTPERRALAAALETMAGEVVEIA
                     PRIDGRPVATGKTAEAVMPHDHRHVLAVWHKAGAKEVEQAIQAALAAHRDWSRTPWPA
                     RAAIFLRAAELLAGPFRQRLNAATMLGQSKTTHQAEIDAACELVDFWRFNVHYATDIY
                     RQQPCSAPGTWNALEYRPLEGFVFAVTPFNFTSIGGNLPTAPAIMGNTVVWKPASSAV
                     YSAHFIMEVLEAAGLPPGVINMVPGAGAEVGDPALASADLAGIHFTGSTATFQAMWET
                     VGGNVRRYASYPRIVGETGGKNFVLAHPSADVAALATALTRGAFEYQGQKCSAASRAL
                     IPVSLWPRVEAMLREQIAGIRVGDPADFTNFMGAVIDRAAFESIQGYIEWARRSPDCE
                     ILVGGRCDDRTGFFVEPTVVRAARPDVKLMREEIFGPVLTVYVYEDRDLDEALAICDR
                     GTPYALTGAVFARDRGAIVRITEALTHAAGNFYINDKPTGAVVGQQPFGGARASGTND
                     KAGSPFNLLRWASPRVIKETFVPPTDFRYPFMQPGE"
     gene            complement(2322..3956)
                     /locus_tag="VLF19_03635"
     CDS             complement(2322..3956)
                     /locus_tag="VLF19_03635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HSE92373.1"
                     /translation="MRAADLIVRCLENEGVRYVFGLPGEEILDILDALADSSARFVLT
                     RHEQGAAFMADAYGRLTGRAGVCLATLGPGATNLATGVADANLDRAPLVALTGQAGRD
                     RIHKESHQYVDIVAHLRPLTKWNTRVETASVVPEVIRKAFKLAESEKPGACHVEVPED
                     VAAEPTDGTPLSTERARRPSPDRLALQMAARLIEAAAFPLIFAGNGVIRGRASGALRE
                     LARGHGIPVAHTFMAKGSMPYDDELCLLTAGLQARDYVSCGFDKADVIIAVGYDPVEY
                     APKFWNPEREKQIIHVDFTPAEVDSYYQPAVEVVGDVREALELLGGLVKGQKDPEAYR
                     PLRRVILEELAEGASDDTWPLKPQRVLQELRAALGREDIVVSDVGTHKLWIARTFPAY
                     EPNTVLISNGYAAMGFALPAAIAAKLVHPERRVVAVSGDGGFLMNCQELETARRMGLA
                     VVTVIFRDDAYNLTEWKQQTRFGRTSGVAFSNPDFVALAAAFGAKGHRVESARELRPI
                     LADALAQPGPSIVDVPVDYRENAKLTARLGHIVCPI"
     gene            complement(3962..5326)
                     /locus_tag="VLF19_03640"
     CDS             complement(3962..5326)
                     /locus_tag="VLF19_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSE92374.1"
                     /translation="MSLQSINPATGEVEETFEETSAGEVERMLATAHAAFLEWRTVPH
                     ATRAKPMRHAAAALRARRTDHARTMALEMGKPIAQGEAEVDKCAWACEYYAEHAEAFL
                     AEQPRATDASRSYVRFDPLGPILAVMPWNFPFWQVFRFAAPALMAGNAAILKHASNVP
                     RCALSIEEVFRDAGFPRGLFATALVGSSAVAGLISDPRVAAVTLTGSDHAGSKVGEQA
                     GRVLKKTVLELGGSDPFVVLGDADLAAAARTAADARLVNSGQSCIAAKRFIVVEPVAD
                     QFIARFADELRSRRVGDPLAHETQVGPQARVDLRDALHRQVEESVRRGAKLLLGGQIP
                     AGPGAFYPPTLLVGVDRGMPAFDEETFGPVAAVVRAKDEADAVRLANDSMFGLGASVW
                     TRDRARAERMAGQIEAGAVFVNGLVKSDPRLPFGGIKRSGYGRELSEYGIREFVNIKS
                     VWIA"
     gene            complement(5635..>7557)
                     /gene="lon"
                     /locus_tag="VLF19_03645"
     CDS             complement(5635..>7557)
                     /gene="lon"
                     /locus_tag="VLF19_03645"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSE92375.1"
                     /translation="HVLYFVASVVPLGVAARQELLETDPITAKLRRLVDLLQRELAVR
                     ELGRKITTDTEERLGKRQREHYLREQLRSIQRELGEEEGGEGDVAELRRRIEEAGLAD
                     EARREAERELKRLAGIPASSPEHGMIHTYLEWMASLPWTKLSGGAIDIHRARRVLDED
                     HFDLEKIKDRILEYLAVKKLRQDRDGREPAPAEPPRVTGDTPAREPILCFVGPPGVGK
                     TSLGQSIARAMGRRFVRMSLGGVRDEAEIRGHRRTYIGALPGRIVQGLRRAETRDPVF
                     MLDEIDKIGADWRGDPSSALLEVLDPAQNHTFVDNYLGVSFDLSQVFFIATANTLDTI
                     PGPLRDRMEILMLSGYTDEEKIGIATQYLIPKQLAAHGLAADELAFEPEALRRMVRGY
                     TREAGVRDLERQIAAVARKVARRIAEGRTGAVRITADDLAEYLGRPRFFDEVAERTTR
                     PGVATGLAWTPTGGDVLFVEVSMMPSSEERLVLTGMLGDVMRESAQAAVSYVWSNAGA
                     LDIDPKLFEARTIHVHVPAGAIPKDGPSAGVTIVTALASLATRRPVRSDVAMTGEITL
                     RGKVLPVGGIKEKVLAAHRAGIRSVILPRLNERDVEDVPEELRRKLSFIFVDDAEEVL
                     RHALTSSATETRLTAR"
BASE COUNT         1088 a         2729 c         2634 g         1106 t
ORIGIN      
        1 gtacaccggc tccgcgggct ggatgtaccg ggccgggctc gagtcgctgc tcggattccg
       61 gctgcgcgga gccgtccttc acctcgatcc gtgcattcca cgggcgtggc gacgcttcga
      121 catcacgttc cgataccacg cctcgcgcta cgtgatcacg gtcgagaacc cgagcggcgt
      181 gacgcgcggc atctccacca tcgagatcga tggaacggcg ctgccgcccg gccccgcgag
      241 gctcgcgctg atggacgacg tcgcgactca ccgcgttcgc gtggtcctcg ggtgagcctc
      301 ggccgggctc ccgcgtggcg ctacaatcga cgaggagtga ggagctcgat ccatgacgaa
      361 caccatcctg agcgttcccg agccccggaa cgagcccgtg ctgtcctacg ctcccggcac
      421 gcccgagcgc cgcgcgctgg cggccgcgct cgagacgatg gcgggcgagg tcgtcgagat
      481 cgcgcctcgc atcgacgggc gcccggtcgc cacgggcaag accgccgagg ccgtgatgcc
      541 gcacgaccac cgccacgtcc tcgctgtctg gcacaaggcg ggagcgaagg aggtggagca
      601 ggcgatccag gcggcgctcg ccgcgcaccg cgactggtcg cggacgccct ggccggcgcg
      661 cgcggcgatc ttcctcaggg ccgccgagct gctcgccggc cccttccggc agcggctgaa
      721 cgcggccacg atgctcgggc agtcgaagac cacccaccag gccgagatcg acgccgcgtg
      781 cgagctcgtc gacttctggc gcttcaacgt ccactacgcc acggacatct accgccagca
      841 gccctgctcg gcgccgggca cgtggaacgc gctcgagtac cgaccgctcg aggggttcgt
      901 cttcgcggtg acgcccttca acttcacctc gatcggcggc aacctgccga ccgcacccgc
      961 gatcatgggt aacacggtcg tgtggaagcc cgcgtccagc gccgtctact cggcccactt
     1021 catcatggag gtgctggagg cggccgggct cccgccgggt gtgatcaaca tggtaccggg
     1081 cgccggtgcc gaggtcggcg atcccgcgct ggcctccgcc gacctcgcgg gcatccactt
     1141 cacgggatcg acggccacgt tccaggcgat gtgggagacg gtcggcggca acgtccgccg
     1201 ctacgcgtcc tacccgcgca tcgtgggtga gacgggcggc aagaacttcg tgctcgccca
     1261 cccctccgcc gacgtcgccg cgctggccac ggccctcacg cgcggcgcgt tcgagtacca
     1321 ggggcagaag tgctcggccg cctcgcgcgc gcttattccg gtctcgctct ggccgcgggt
     1381 ggaggcgatg ctgcgcgagc agatcgccgg gatccgcgtg ggcgacccgg ccgacttcac
     1441 gaacttcatg ggggccgtca tcgaccgcgc cgccttcgag agcatccagg gctacatcga
     1501 gtgggcccgg cgctcgccgg actgcgagat cctggtcggc ggccggtgcg acgaccgcac
     1561 ggggttcttc gtggagccga cggtggtgcg ggcggcgagg cccgatgtca agctgatgcg
     1621 agaggagatc ttcggcccgg tgctcacggt gtacgtctac gaggatcgcg acctcgacga
     1681 ggccctggcg atctgcgacc gcgggacgcc ctacgcgctc acgggagcgg tgttcgcccg
     1741 cgaccgcggc gcgatcgtcc ggatcaccga ggccctcacc cacgccgccg gcaacttcta
     1801 catcaacgac aagccgaccg gcgcggtggt cggccagcag ccgttcggcg gcgcccgcgc
     1861 ttcgggcacg aacgacaagg cgggcagccc gttcaatctc ctccgctggg cgagtccgcg
     1921 cgtgatcaag gagaccttcg tgcccccgac cgactttcgc tatccgttca tgcaaccggg
     1981 ggagtagttc atcggagggg ggcctcgacg atccctccga gcctccccaa gagatcgttg
     2041 cggcggccga gccgtcaccc ggatacgttg tcagccatag cgagtccccc tgagtcgcaa
     2101 cttcgccgac cgtaggccgg ggtggggtcg gggggcgcgg gtccgccgac ctgttccccg
     2161 cccacgaaac ctcaccccat ccgaagatgg aaacggcacc agagcgacga cgcgcagcgc
     2221 atcaggggtc ggcggacccg cgccccccga ccccaccccg gccggcgcgt tcaccggcaa
     2281 agttctgatt cacacgccag agccggcgac agcgccagcg gtcagatggg gcagacgatg
     2341 tggccgagcc gtgccgtcag cttggcgttc tcgcgatagt ccacgggcac gtcgacgatc
     2401 gagggaccgg gctgggccag cgcgtcggcc aggatcggcc ggagctcgcg cgccgactcg
     2461 acccgatgac ccttggcgcc aaaggccgcg gccagcgcga cgaagtccgg gttggagaag
     2521 gcgacgcctg aggtccggcc gaagcgcgtc tgctgcttcc actcggtgag gttgtaggcg
     2581 tcgtcccgga agatgacggt gacgacggcc agccccatgc gccgcgcggt ctcgagctcc
     2641 tggcagttca tgaggaagcc gccgtccccg ctcaccgcga ccacccggcg ctcggggtgg
     2701 acgagcttgg cggcgatggc cgccggcagc gcgaatccca tcgccgcgta gccgttggag
     2761 atcagcacgg tgttcggctc gtaggcggga aaggtccggg cgatccagag cttgtgcgtg
     2821 ccgacgtccg agacgacgat gtcctcgcgc cccagcgcgg cccggagctc ctgcaagacg
     2881 cgctggggct tgagcggcca cgtgtcgtcg ctcgcgccct cggcgagctc ctcgaggatg
     2941 acgcgccgga ggggccggta agcctccggg tccttctgac ccttgacgag ccccccgagg
     3001 agctcgaggg cctcccgcac gtcccccacg acctcgaccg ccggctggta atagctgtcc
     3061 acctcggccg gcgtgaagtc gacatggatg atctgcttct cgcgctcagg gttccagaac
     3121 ttgggcgcgt actcgacggg atcgtagccg acggcgatga tgacgtcggc cttgtcgaac
     3181 ccgcacgaca cgtagtcccg ggcctggagt cccgccgtga gcaggcacag ctcgtcgtcg
     3241 tagggcatcg agcccttggc catgaacgtg tgcgcgacgg gaatgccgtg gccgcgggcg
     3301 agctcgcgga gcgccccgga ggcgcgcccg cggatgacgc cgttgcccgc gaagatcagc
     3361 gggaacgccg ccgcctcgat caggcgggcc gccatctgca gggccagccg gtccggcgac
     3421 gggcgccgcg cccgctccgt ggagagcggc gtgccgtcgg tcggctccgc ggcgacgtcc
     3481 tccggcacct cgacgtggca ggccccgggc ttctccgact cggccagctt gaaggccttg
     3541 cggatcacct cgggcaccac gctggcggtc tcgacccggg tgttccactt cgtcagcggg
     3601 cgcaggtgcg cgacgatgtc cacgtactgg tgggactcct tgtggatccg atcgcggccc
     3661 gcctgccccg tcagggcgac gagaggcgca cggtcgaggt tcgcgtcggc gacgccggtg
     3721 gcgaggttcg tcgcgccggg gcccagcgtg gcgaggcaga cgccggcgcg gccggtgagg
     3781 cgcccgtagg cgtcggccat gaacgccgcg ccctgctcgt ggcgcgtgag cacgaagcga
     3841 gccgaggaat ccgcgagcgc gtccagaatg tcgaggatct cctcgcccgg cagaccgaag
     3901 acataccgga ccccctcgtt ctcgagacag cggacgatca ggtcggcggc ccgcatggcg
     3961 gctacgcgat ccacaccgac ttgatgttca cgaactcgcg gatgccgtac tccgacagct
     4021 cgcgcccgta gcccgagcgc ttgatcccgc cgaacggcag gcgagggtcg gacttcacga
     4081 ggccgttcac gaacaccgcg ccggcctcga tctgccccgc catgcgctcg gcgcgcgcgc
     4141 ggtcccgggt ccacaccgag gcgccgagcc cgaacatcga gtcgttggcg aggcggacgg
     4201 cgtcggcctc gtccttggcc cggaccaccg cggcgacggg gccgaaggtc tcctcgtcga
     4261 acgccggcat gccccggtcc acgccgacga gcagcgtcgg cggatagaag gcgcctgggc
     4321 cggcagggat ctggccgcca aggagcagct tcgccccgcg cctgaccgat tcctcgacct
     4381 gccggtgcag cgcgtccctg aggtccactc gggcctgcgg cccgacctgc gtctcgtgtg
     4441 ccagcggatc gccgacccgg cgggaccgga gctcgtcggc gaagcgcgcg atgaactggt
     4501 cggcgacggg ctcgacgacg atgaaccgct tggcggcgat gcagctctgg ccgctgttga
     4561 cgagcctcgc gtcggccgcg gtccgggcgg cggcggcgag atcggcgtcg ccgagaacga
     4621 cgaaggggtc gctgccgccg agctcgagca cggtcttctt cagcacgcga cccgcctgct
     4681 cgccgacctt gcttcccgcg tggtcactgc ccgtcagcgt gaccgcggcg actcgcggat
     4741 cagagatgag gccggcgacc gccgaggagc cgaccagcgc ggtcgcgaac agcccccgcg
     4801 ggaagccggc gtcccggaaa acctcctcga tcgagagggc gcaccggggc acgttggacg
     4861 cgtgcttgag gatcgccgca ttgcccgcca tcagcgcggg cgcggcgaag cggaacacct
     4921 gccagaacgg gaagttccag ggcatcacgg cgaggatggg ccccagcgga tcgaagcgga
     4981 cgtagctgcg cgaggcatcc gtcgcgcgcg gctgctccgc gaggaaagcc tcggcgtgct
     5041 cggcgtagta ctcgcaggcc cacgcgcact tgtcgacctc cgcctcgccc tgcgcgatcg
     5101 gcttacccat ctcgagggcc atggtccgcg cgtgatccgt ccggcgcgcg cgcagcgcgg
     5161 ccgccgcgtg gcgcatcggt ttggcacgcg tggcgtgggg aacggtgcgc cactcgagaa
     5221 acgcagcatg ggcggtcgcg agcatccgct cgacctcccc cgcagaggtc tcctcgaacg
     5281 tctcctccac ctcgcccgtt gccggattga tcgactgcaa gctcatggct ttccacctcc
     5341 gcgaccaggc tgcctccagc ctgccgcatc cagaggccgg ttgccacctc tggcccaccg
     5401 tgggaactgc acggagacgc ttgacgacct ggtccccggt cacgctccca ggcccacggc
     5461 cgaggacggc ggtgtcaccc tcgacgtcat cgaccggcat gggttgctgc gcgcctcgcc
     5521 gaatgatggc ggttcgtgtg tggacggggg gacacggtcg agcaggatga tccggtatct
     5581 cggtcttgcg cggaggacgc cggccggagt ccggctcctt cacgcacgat caggtcaccg
     5641 ggccgtcagc ctcgtctcgg tcgccgagga cgtgagggca tgccgcagga cctcttcggc
     5701 gtcgtcgacg aagatgaagg acagcttccg gcgcagctcc tccggcacgt cctccacgtc
     5761 ccgctcgttc aggcgcggca ggatcaccga gcggatcccc gcccggtgcg cagccaggac
     5821 cttctccttg atccccccaa cgggaagcac cttcccgcgc agggtgatct cgcccgtcat
     5881 ggccacgtcg ctccgcaccg gccgccgcgt ggccagcgag gccagcgcgg tcacgatggt
     5941 caccccggcc gaggggccgt ccttggggat ggccccggct ggcacgtgca cgtggatggt
     6001 cctggcctcg aagagcttcg ggtcgatgtc gagagcgccg gcgttcgacc acacgtagga
     6061 cacggcggcc tgggcgctct cgcgcatgac gtccccgagc atgccggtga ggacgagccg
     6121 ctcttcgctg ctcggcatca tcgacacttc gacgaagagg acgtccccgc ccgtcggtgt
     6181 ccaggccagc cccgtggcga ccccggggcg cgtggtccgc tcggccacct cgtcgaagaa
     6241 ccgcggtcgg ccgagatact ccgccaggtc gtccgcggtg atccggactg cgccggtccg
     6301 gccctcggcg atccgccggg ccaccttccg ggccacggcg gcgatctgcc gctcgaggtc
     6361 tctcacgccc gcctcgcggg tgtacccgcg caccatccgg cgaagggctt cgggctcgaa
     6421 ggcgagctcg tccgcggcga ggccgtgggc ggcgagctgc ttcgggatca gatactgggt
     6481 ggcgatcccg atcttctcct cgtccgtgta cccggacagc atcaggatct ccatgcggtc
     6541 acgcaggggg ccggggatcg tgtcgagggt gttcgcggtg gcgatgaaga acacctgcga
     6601 gaggtcgaac gacaccccga ggtagttgtc cacgaaggtg tggttctgcg cgggatcgag
     6661 cacttccagg agggcagagg agggatcgcc ccgccagtcc gctccgatct tgtcgatctc
     6721 gtcgagcatg aagacgggat cacgggtctc ggcccgcctc agcccctgga cgatccggcc
     6781 ggggagcgcg ccgatgtagg tccggcgatg accgcggatc tccgcctcgt cgcggacgcc
     6841 gcccagcgac atgcgcacga accgccggcc catggcccgg gcgatgctct gccctaggct
     6901 ggtcttgccc acgcccggcg gccccacgaa gcagagaatc ggctcgcgcg ccggggtgtc
     6961 gcccgtcacg cgcggaggct cggcgggcgc cggctcccgg ccatcgcggt cctggcggag
     7021 cttcttgacg gccaggtatt cgaggatgcg atccttgatc ttctcgaggt cgaagtggtc
     7081 ctcgtcgagc acccggcggg cccggtggat gtcgatggcg ccgccgctca gcttggtcca
     7141 cggcaggctc gccatccact cgaggtacgt gtggatcatg ccgtgttccg gcgaggacgc
     7201 cgggatgccg gccagccgct tcaactcgcg ctccgcctca cgccgggcct cgtccgccag
     7261 gccggcctcc tcgatccggc ggcgcagctc ggccacgtcg ccctcgcctc cttcctcctc
     7321 cccgagctcc cgctgaatgg accgcagctg ctcgcgcagg tagtgctctc gctgccgctt
     7381 ccccagccgc tcctcggtgt ccgtcgtgat cttgcggccc agctcgcgca cggcgagctc
     7441 gcgctgcaac aggtcgacga gtcgccggag cttggccgtg atcggatccg tctccaggag
     7501 ctcctgccgt gcggccacgc cgagcgggac caccgacgcg acgaagtaca gcacgtg
//
LOCUS       DATIAZ010000106         4855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_684477, whole genome shotgun sequence.
ACCESSION   DATIAZ010000106 DATIAZ010000000
VERSION     DATIAZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684477"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VLF19_03650"
     CDS             <1..197
                     /locus_tag="VLF19_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92376.1"
                     /translation="ERLLQIVELERLDDGLDLFHRFPPGRMWSRAMTVPALSTPGPPV
                     KSTSWIAWDGGRATKAMLSE"
     gene            complement(428..1789)
                     /locus_tag="VLF19_03655"
     CDS             complement(428..1789)
                     /locus_tag="VLF19_03655"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE92377.1"
                     /translation="MLVVDDEPSIVDGLRLTLEAEGYAVRIAGSVQTALSVVMQSDVQ
                     AAIVDLMLPDGDGLLLTRELKKRDPALEVIVVTAYGSVRKAMEATKGAGAFYVLEKPF
                     DPDEVLGLVKNAVEHRKLVAENLDLKRRLGEQAADSEVLHSAPAMKRVMETVTSVADT
                     DANVLIVGESGTGKELIANALHDKSRRRDGPWVKINCAALPKDLIESELFGHTKGSFT
                     GATTEKTGLLEEAHRGSLLLDEITEMPVELQAKLLRVLEERVVRRLGGTRAVPVDFRL
                     ISSTNRSAEQAVKDGGLRQDLYFRINTVTIAVPPLRERRDDIPLLVRAFLERYCAKHD
                     RPVQAIEPEAYRRLLAYAWPGNVRELQHAIEHGVLVARSSQITLADLPEPLQRVGGDA
                     PAAMIAPASVPAGSLEEIERASILKALESTRWNKQAAAGLLGLRRPTLYSKMRKHGIP
                     QRP"
     gene            complement(1804..3315)
                     /locus_tag="VLF19_03660"
     CDS             complement(1804..3315)
                     /locus_tag="VLF19_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92378.1"
                     /translation="MKRRVLVVDDDAAILEVLEMRLAAMGFDVTATTEAAGALEASDS
                     GRFDLALLDLRMEPVDGIRLMEALHARQPRLPVLIMTAHGTIETAVDAVQRGAFDYLT
                     KPFVRDELRAKIRRALAARRWARDRERLITVGETFASSGVMSRILDAIAQAAVETTEA
                     ERSVVFQLQSGRLVPMASAGSPPPSWSALEGAATRAIEKGAPLTVAGAESHVIAAAPL
                     VVQRGPVGALVIETPARVELTEDDLDLLALFSSQAAIAIRNTHELERLRSGALAALGR
                     MATQVAHELKNPLAGLRLYAHHLEQRLVDGGDATSAELARKVTSTVDHLASVVSEITS
                     FGRQPELKRAPTALGPLLDECVTLARARCPADGIEVVTTYDDACPDVEADPRELRKAF
                     LNLILNALEALEHRGRLTVATAWSADTGVVTVSIDDTGPGMSDETLARAFDLFFTTKP
                     EGTGLGMAVARAVVDLHGGELTVQSAPATGTRVRVRLPGRLPAPAGGTATEPK"
     gene            complement(3312..3695)
                     /locus_tag="VLF19_03665"
     CDS             complement(3312..3695)
                     /locus_tag="VLF19_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE92379.1"
                     /translation="MLALALTGCAPSPQALDHAAAQNVLAQADSLLEQGEYAAAVQAY
                     DEALARHPGDRDASRARASRNTVARLIAARADAARLRDALAARDGELTRLRQEVQRLT
                     QETDRLRTNLETLKEIDLRQERRRK"
     gene            complement(3713..>4855)
                     /locus_tag="VLF19_03670"
     CDS             complement(3713..>4855)
                     /locus_tag="VLF19_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSE92380.1"
                     /translation="REVPLLRDVRGAFDAYQRVVEEEQALLREGRSEQARRLAEVESR
                     RLSDRAESAAERLIDAIHAAAFTAQAEAARLEVRTWTIVLSSLGAAFAVALAGAAVIA
                     HRLTRSLRELSAATAAVAEGAYRPAPVKGRDEVAALARAFNAMAERLRELDQLKEGFL
                     ATLSHELRSPLTSMREGAHLLRDEVAGGLTPKQRRLVVIIQEGSERLLRLVNQLLDLS
                     RLRAGMLPIERMPVVLDHLVQRAVEELRPQAEEAGVAVEVERRGERFQYLGDADRLVQ
                     VLVNLVANAVRFTPRGGRVTVRVADAGPELIVEVEDTGVGIPAAQLPQIFGWYEQAHR
                     HRGGTGLGLPIARGLVEAHGGRLTVESQEGKGSRFTVLLPREGVTR"
BASE COUNT          636 a         1775 c         1747 g          697 t
ORIGIN      
        1 gtgagcgcct ccttcagatc gtcgagcttg aacggcttga tgatggcctc gatctttttc
       61 atcggttccc ccccgggcgc atgtggagcc gagcaatgac tgtgcctgcc ctttccaccc
      121 cagggccgcc tgtaaaatca acgagttgga tagcatggga cggcggccgt gcgaccaagg
      181 cgatgctcag cgaataggca gcaccagctg aactgcccgc gtttcagacc cgccgttctg
      241 ggctttccct gggcgcccgg ccggcccagc gtgcaccacg gggcgaagga cgtcgtccga
      301 gaatcccgag tgcacgccgg gagtcgggtg cgcgccggcc gtgcggcgca acccgaatca
      361 tggcgaggtt tcggacggga ccgtcgaggc cccctccgac gaggatgggg gcgaggcccc
      421 tccgggttca gggccgctgc gggatcccgt gcttgcgcat cttcgagtag agcgtgggcc
      481 ggcgcagccc caggagaccg gcggcggcct gcttgttcca gcgcgtgctc tcgagcgcct
      541 tgaggatcga cgcccgctcg atttcctcga gagagcccgc cggcaccgac gccggcgcga
      601 tcatcgccgc gggcgcgtcg ccacccacgc gctgcagcgg ctcgggcagg tcggcaaggg
      661 tgatctggga cgagcgggcc accaggactc cgtgctctat tgcgtgctgt agctcgcgca
      721 cgttccccgg ccaggcatag gccaggagcc gccggtacgc ctcgggctcg atcgcctgca
      781 cgggccggtc gtgcttcgcg cagtagcgct cgaggaaggc gcgcacgagc aaggggatgt
      841 cgtcgcggcg ctcgcgaagc ggcgggacgg cgatggtcac cgtgttgatg cggaagtaga
      901 ggtcctgccg gagccctccg tccttcacgg cctgctcggc gctccggttg gtggacgaga
      961 tgagccggaa gtccacgggc accgcccgcg tgccgccgag ccgccgcacg acgcgctcct
     1021 cgaggacccg cagcagcttg gcctggagct cgacgggcat ctcggtgatc tcgtcgagca
     1081 gcagcgaccc ccgatgcgcc tcctcgagca ggccggtctt ctccgtggtc gccccggtga
     1141 acgatccctt cgtgtggccg aagagctcgg actcgatgag gtccttgggc agcgcggcgc
     1201 agttgatctt cacccacggc ccgtcgcgcc ggcggctctt gtcgtggagc gcgttggcga
     1261 tcagctcctt gcccgtcccg ctctcgccca cgatcaggac gttggcgtcg gtgtcggcca
     1321 cggaggtcac ggtctccatg acccgcttca tcgccggcgc ggagtgcagc acctcgctgt
     1381 cggccgcctg ctccccgagc cgtcgcttga ggtcgaggtt ctcggcgacc agcttgcgat
     1441 gctccacagc gttcttgacg agacccagga cctcgtcagg gtcgaagggc ttctcgagga
     1501 cgtagaacgc gcccgcgccc ttggtggcct ccatggcctt ccgcaccgag ccgtaggcgg
     1561 tgacgacgat gacctccagg gcggggtcgc gtttcttcag ctcgcgggtg agcaggaggc
     1621 cgtcgccgtc cggcagcatg aggtcgacga tggcggcctg gacgtcgctc tgcatcacca
     1681 cggacagcgc cgtctgcacg ctgccggcga tccgcacggc gtagccctcg gcctcgagcg
     1741 tgaggcggag gccgtccacg atggacggct cgtcgtcgac caccagcacg gtacgaccgt
     1801 gcctcacttc ggttccgtgg cggtgccgcc ggcgggcgcc ggcaggcggc cgggcaggcg
     1861 cacccgcacg cgggtcccgg tggccggcgc gctctgcacg gtgagctcgc cgccgtgcag
     1921 gtcgaccacc gcgcgggcga cggccatgcc gagaccggtg ccctccggct tcgtcgtgaa
     1981 gaagaggtcg aacgcgcgcg cgagcgtctc gtcgctcatg ccggggccgg tgtcgtcgat
     2041 cgacaccgtc accacgcccg tgtccgccga ccaggccgtg gccacggtga gccgcccgcg
     2101 gtgctccagg gcctcgagcg cgttcaggat caggttgagg aacgccttgc gcagctcgcg
     2161 cgggtcggcc tccacgtcgg ggcaggcgtc gtcgtaggtg gtcacgacct cgatgccgtc
     2221 cgcgggacag cgcgcgcgcg ccagcgtgac gcactcgtcg aggagcggcc cgagcgcggt
     2281 gggcgcccgc ttgagctccg gctggcggcc gaaggacgtg atctccgaca ccaccgaagc
     2341 caggtgatcg accgtcgagg tcaccttccg cgccagctcc gcgctggtcg cgtctccgcc
     2401 gtccacgagg cgctgctcga ggtggtgggc gtagagccgg agcccggcca gcgggttctt
     2461 cagctcgtgg gcgacctgcg tcgccatccg ccccagcgcc gcgagggccc cgctgcgcag
     2521 ccgctcgagc tcgtgcgtgt tgcggatggc gatggcggcc tgcgaggaga agagcgcgag
     2581 caggtcgagg tcgtcctccg tgagctccac ccgggcgggc gtctcgatga ccagcgcgcc
     2641 cacgggcccg cgctggacca ccagcggagc ggccgcgatc acgtgcgact cggcgccggc
     2701 gacggtgagc ggtgcgccct tttcgatggc gcgggtggcc gcgccctcca gcgcgctcca
     2761 cgacggcggc ggcgagcccg cgctggccat cggcacgagc cgtccgctct ggagctggaa
     2821 gaccacggag cgctcggcct cggtcgtctc cacggccgcc tgggcgatgg cgtcgaggat
     2881 acgcgacatc acgccggacg acgcgaacgt ctcgccgacg gtgatgaggc gctcgcggtc
     2941 gcgggcccag cgccgcgccg cgagcgcccg gcggatcttg gcccgcagct cgtcgcggac
     3001 gaaaggcttc gtcaggtagt cgaaggcgcc gcgctggacg gcgtccaccg ccgtctcgat
     3061 ggtgccgtgc gcggtcatga tgagcaccgg cagccgcggc tgccgggcgt gcagcgcttc
     3121 catgaggcgg atgccgtcca cgggctccat gcgaaggtcg agcagcgcga ggtcgaaccg
     3181 cccgctgtcg ctggcctcga gcgcgccggc cgcctcggtc gtcgcggtga cgtcgaagcc
     3241 catcgcggcc agccgcatct cgagcacctc gagaatcgcg gcatcgtcgt cgaccaccag
     3301 cacccggcgc ttcatttccg gcgacgttcc tggcgcaagt cgatttcctt cagcgtctcg
     3361 agattggtgc gaagacggtc ggtctcctgt gtgaggcgct gcacctcctg gcgcaggcgc
     3421 gtgagctcgc catcgcgcgc cgccagcgcg tcgcgcaggc gggcggcgtc cgcgcgcgcc
     3481 gcgatcaggc gcgccacggt attgcgcgac gcccgggcgc gcgaggcgtc gcggtcgccg
     3541 ggatgtcggg cgagcgcttc gtcgtaagct tgcaccgccg ccgcgtactc cccctgctcg
     3601 aggagcgagt ccgcctgcgc caggacgttc tgcgccgcgg cgtggtccag cgcctgcggc
     3661 gacggagcgc agccggtcag ggccagcgcg agcaccgcgg tcgcgatcga cctcacctgg
     3721 tcaccccttc gcgaggcagc aggaccgtga accggctgcc cttgccttcc tgggattcta
     3781 ccgtcagccg gcctccgtgg gcctccacaa ggccgcgggc gatcggcagg ccgagcccgg
     3841 tgccgccacg gtgccggtgc gcctgctcgt accagccgaa gatctgaggc agctgcgcgg
     3901 ccgggatccc gacgccggtg tcctcgacct cgacgatgag ctcgggaccg gcgtcggcca
     3961 cgcgcacggt cacccgcccg ccgcgcggcg tgaaccgcac cgcgttggcc acgaggttca
     4021 ccaggacctg cacgagccgg tcggcgtcgc ccagatactg gaagcgctcg ccgcggcgct
     4081 cgacctcgac cgccaccccc gcctcctcgg cctgtggccg cagctcctcc acggcgcgct
     4141 gcaccaggtg gtcgagcacc accggcatgc gctcgatcgg cagcatcccg gcccgcaggc
     4201 gcgagaggtc gaggagctgg ttgacgagcc ggagcaggcg ctcggagcct tcctggatga
     4261 tcacgaccag gcgcctctgc ttcggcgtca gcccgccggc cacctcgtcg cgcagcaggt
     4321 gcgcgccctc gcgcatcgac gtcagcggcg agcgcagctc gtgagacagc gtcgccagga
     4381 acccctcctt gagctggtcg agctcccgca gccgctcggc catggcgttg aacgcgcggg
     4441 cgagggcggc gacctcgtcg cggcccttga cgggcgccgg ccggtaggcg ccctccgcca
     4501 cggccgcggt cgccgccgac agctcgcgca gcgagcgcgt gagccggtgg gcgatcacgg
     4561 ccgcgccggc cagcgcgacg gcgaacgccg cgcccagcga gctgagcacg atcgtccacg
     4621 tgcgcacctc gagccgcgcc gcctcggcct gcgcggtgaa ggccgcggcg tggatggcgt
     4681 cgatgagccg ctccgccgcc gactcggcgc gatccgacag gcggcgcgac tcgacctcgg
     4741 ccaggcgccg cgcctgctcg ctgcggccct cgcggagaag cgcctgctct tcctccacga
     4801 cgcgctggta ggcgtcgaac gcgccccgca cgtcccggag cagcggcacc tcgcg
//
LOCUS       DATIAZ010000107         3868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_684537, whole genome shotgun sequence.
ACCESSION   DATIAZ010000107 DATIAZ010000000
VERSION     DATIAZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684537"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VLF19_03675"
     CDS             <1..394
                     /locus_tag="VLF19_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92381.1"
                     /translation="EPAGAAMANELILIVEDNAKNRKLVRDVLQFKGYQTLEAETGED
                     GVRLAREHRPALVLMDIQLPGIDGITALGQLRSDAGTRAIPVLAVTASAMTHDRQKIL
                     AAGFDGYQTKPIRVTEFLDAVRALLDRR"
     gene            516..1613
                     /locus_tag="VLF19_03680"
     CDS             516..1613
                     /locus_tag="VLF19_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92382.1"
                     /translation="MTAPARLLVVDDTPNNVKLLADLLRARGYEVATASSGAEALARV
                     ETERPDLVLLDVVMPEMSGYEVCRRIRANSATTALPVVMVTALDPTEERIKGIEAGAD
                     DFLTKPINQPELLARVRSLLRIKHLHDELVALNRTLEARVIEQVTQLERLGRLKRFFS
                     PQLAELIVSGGADDPLRSHRREVTVVFLDLRGFTAFAETTEPEEVMGVLREYHAEMGR
                     LILEHEGTLERFTGDGMMIFFNDPVPVDDAPLRAVRMALAMRERVGELLAKWRKRGFE
                     LDFGVGIAQGYATIGAIGFEGRWDYGAIGTVTNLAARLCGEAKPGQVLVAQRVRGAVE
                     DHAQTEPVGELTLKGFSRPVPVFNVVAVSES"
     gene            complement(1648..2103)
                     /locus_tag="VLF19_03685"
     CDS             complement(1648..2103)
                     /locus_tag="VLF19_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSE92383.1"
                     /translation="MTVYLDDFKPGDTQEFPGRYTFTREAIVTFAREYDPQPFHVDEA
                     AAARSIYGGLIASGWQTVGVTFRLAVDGLINRTASMGSPGVDEVRWLRPVRPGDTVKV
                     RVEVLEVRPSRSKPDRGLVRVRYETSNQRDEVVLTMVGLGLFARRPRLG"
     gene            complement(2139..2708)
                     /locus_tag="VLF19_03690"
     CDS             complement(2139..2708)
                     /locus_tag="VLF19_03690"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HSE92384.1"
                     /translation="MSLRALIRAAALAAVLALAALEVAWGQTADGPGPDGLAPDERAA
                     VERHRERWERMTPEERQRAVERYRRWRAMSPGERQAWRERRERMRRLSPDERSRILKD
                     LQRWDTLPAERRRELNDVHEHFQRLPQERRERIMERLRQYEAMSPEERERLARNWERW
                     RTMSPEQREQLRERWMRMTPEERRQLRER"
     gene            complement(2708..3160)
                     /locus_tag="VLF19_03695"
     CDS             complement(2708..3160)
                     /locus_tag="VLF19_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSE92385.1"
                     /translation="MDHPRTELIPYLRGELPPVERESVETHLAGCRACRAERDAFAEL
                     LLDLRRSLPETPPLDWARWHADLRVRLRRRARRPWLAPLPLAAASATLAAGLVVVLWL
                     GVERHAPPPEMVVAEAPMLMEPVEALAAELPEDIDLIVQLDRLTGGDG"
     gene            complement(3160..3729)
                     /locus_tag="VLF19_03700"
     CDS             complement(3160..3729)
                     /locus_tag="VLF19_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSE92386.1"
                     /translation="MEPSDEALCERVARGEEAAFDLLVGRYQQRAWRLAWSMLHHVED
                     ARDVCQDAFVRVFQSAGTFRSQARFSTWFYRIVVNQCIEHRRRGRPWLRFVPFAGRDD
                     EAGDLLERQPAEAVDPIEGLDKEQAVKRMWAAIDGLSARQRAAVVLYAQDELSTREIA
                     SVLSCSEATVRVHLHRALATLRRTMEKVE"
BASE COUNT          562 a         1389 c         1388 g          529 t
ORIGIN      
        1 tgagcctgcc ggagcggcca tggccaacga gctgatcctg atcgtcgagg acaacgcgaa
       61 gaaccggaag ctcgtgcgcg acgtgctgca gttcaagggc taccagaccc tcgaggcgga
      121 gaccggcgag gacggcgtgc ggctggcgcg cgagcatcgc ccggcgctcg tgctcatgga
      181 catccaactc cccggcatcg acgggatcac cgcgctgggg cagctccgga gcgacgccgg
      241 cacgcgcgcc atcccggtcc tggcggtgac cgcctcggcc atgacccacg accgacagaa
      301 gatcctggcc gccggcttcg acggctacca gaccaagccc atccgcgtca ccgagttcct
      361 cgacgccgtg cgcgccctgc tggaccgcag atgagcgggg gcgacggctt gagagggccg
      421 cggtacgggg cccggcgacc acggcgggag tgctcgccga gccgcgaggc gagcgaggcg
      481 atgatcccgg cagccgcgcc agggagcgtc gtccagtgac cgccccggct cgtctgctcg
      541 tcgtcgacga cacgccgaac aacgtgaagc tgctggccga cctgctgcgc gcccggggct
      601 acgaggtcgc cacggcgtcc tcgggcgcgg aggcgctggc gcgcgtggag accgagcggc
      661 cagacctcgt gctgctcgac gtcgtcatgc cggagatgag cggctacgag gtgtgccggc
      721 gcatccgggc gaactcggcg acgacggcgc tgccggtggt gatggtcacc gcgctcgacc
      781 cgacggagga gcggatcaag ggcatcgagg ccggcgccga cgacttcctc accaagccca
      841 tcaaccagcc ggagctgctg gcaagggtgc gctcgctgct ccgcatcaag catctgcacg
      901 acgagctggt ggcgttgaat cgtacgctgg aggcgcgggt catcgagcag gtcacccagc
      961 tcgagcgcct cggccgcctc aagcgtttct tctcgccgca gctcgccgag ctgatcgtgt
     1021 cgggcggcgc cgacgatccg ctcaggagcc accggcggga ggtcacggtc gtcttcctcg
     1081 acctgcgcgg gttcaccgcg ttcgcggaga ccaccgagcc cgaggaggtc atgggcgtgc
     1141 tccgcgagta ccacgccgag atgggccggc tcatcctgga gcacgagggc acgctcgagc
     1201 gcttcaccgg cgacggcatg atgatcttct tcaacgaccc ggtgccggtg gacgacgcac
     1261 cgctgcgcgc ggtgcgcatg gccctggcca tgcgcgagcg cgtcggcgag ctgctcgcga
     1321 agtggcgcaa gcgcgggttc gagctggact tcggcgtcgg aatcgcccag ggctacgcga
     1381 ccatcggcgc catcggcttc gagggacgct gggactacgg cgccatcggg accgtgacga
     1441 atctggcggc gcgcctctgc ggcgaggcca agcctgggca ggtcctcgtg gcccagcgcg
     1501 tgcgcggcgc cgttgaagac catgcgcaga ccgagccggt cggcgagctg acgctcaagg
     1561 gtttctcgcg gccggtgccg gtcttcaacg tcgtcgcagt gagcgagtcc tgaccagccg
     1621 acacggcgcc gagctcggag gagcaggcta gcccaggcgc ggccggcggg cgaacagacc
     1681 caggcccacc atcgtgagca cgacttcgtc ccgctggttc gaggtctcgt accgcacgcg
     1741 gacgagcccg cgatcgggct tgctccgcga gggacggacc tccagcacct ccacgcgcac
     1801 cttcaccgtg tcgcccggcc gcaccggcct gagccagcgg acctcgtcga cgcccggcga
     1861 gcccatgctc gccgtacggt tgatgagacc gtcgacggcc agccggaagg tgacaccgac
     1921 ggtctgccag ccgctggcga tgagcccgcc gtagatcgac cgcgcggcgg cggcctcgtc
     1981 gacgtggaag ggctgggggt cgtactcgcg cgcgaaggtc acgatcgcct cgcgcgtgaa
     2041 cgtgtagcgg ccggggaact cctgcgtgtc gccgggcttg aagtcgtcga ggtagacggt
     2101 catggcctcg ccaggatacg cgaggccccg gcacgcggct atcgttcgcg cagctggcgg
     2161 cgctcctcgg gggtcatgcg catccaccgc tcgcgcagct gctcgcgctg ctccggggac
     2221 atggtcctcc agcgctccca gttgcgggcc aggcgctcgc gctcctccgg cgacatcgcc
     2281 tcgtactgcc gcaggcgctc catgatgcgc tcgcgccgct cctggggcag gcgctggaag
     2341 tgctcgtgga cgtcgttcag ctcgcgccgg cgctcggccg gcaacgtgtc ccaccgctgc
     2401 aggtccttga ggatccggct gcgctcgtcg ggcgagagtc tccgcatgcg ctcgcgccgc
     2461 tcgcgccacg cctgccgctc cccgggggac atggccctcc atcgccggta gcgctcgacc
     2521 gcgcgctggc gttcctcggg cgtcatgcgc tcccagcgct cgcgatggcg ctcgacggcg
     2581 gcgcgctcgt cgggtgcgag cccgtcggga cccggcccat ccgccgtctg tccccacgcc
     2641 acctccagcg cggccagggc cagcacggcg gccagggcgg cggcgcggat cagtgcgcgc
     2701 agggacatca gccgtcaccg cccgtgaggc ggtcgagctg cacgatcagg tcgatgtctt
     2761 ctggcagctc ggcagccagc gcctcgaccg gctccatcag catcggcgcc tcggccacga
     2821 ccatctcggg cggcggcgcg tgtcgctcga ctccgagcca gagcacgacc acgaggccgg
     2881 cggcgagcgt ggcggaggcg gcggccagag gcagcggcgc gagccagggc cggcgggcgc
     2941 ggcgccggag ccgcacgcgc aggtcggcgt gccagcgggc ccagtcgagc ggaggtgttt
     3001 ccgggagcga gcggcgcaga tcgagcagca gctcggcgaa ggcgtcgcgc tcggcccgac
     3061 aggcgcggca gcccgccagg tgcgtctcga cgctctcgcg ctcgacgggc ggcagctcac
     3121 cgcgcaggta ggggatcaac tcggtccgcg ggtggtccat cactcgacct tctccatcgt
     3181 gcgtctgagc gtcgcgagcg cgcgatgcag gtgcacgcgg accgtcgcct ccgagcacga
     3241 gagcaccgac gcgatctcgc gtgtggacag ctcgtcctgc gcgtagagca ccacggccgc
     3301 gcgctggcgg gccgagagcc cgtcgatggc ggcccacatc cgcttcacgg cttgctcctt
     3361 gtccagcccc tcgatcggat cgacggcttc ggccggctgg cgctcgagca ggtcgccggc
     3421 ctcgtcgtcg cgccccgcga aagggacgaa gcgcaaccac gggcgtccgc gacggcggtg
     3481 ctcgatgcac tggttgacga cgatccggta gaaccacgtc gagaagcgtg cctggcttcg
     3541 gaacgtgcct gccgactgga acacgcggac gaaggcgtcc tggcagacgt cgcgcgcatc
     3601 ctcgacgtga tgcagcatcg accacgcgag ccgccaggcg cgctgctgat atcgcccgac
     3661 caggaggtcg aacgccgctt cctccccccg ggccacgcgc tcgcacagtg cctcatccga
     3721 tggctccacg tattcagtgt acttagacga tccagcagat ccgggcgttt acgacccagt
     3781 agtcgaggcg cgccacgtct ccgccggggc gtgccgagcc acccgggggg ctcggggggc
     3841 ccgctcggga ccccccatgt aatcaatc
//
LOCUS       DATIAZ010000108         4427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_816703, whole genome shotgun sequence.
ACCESSION   DATIAZ010000108 DATIAZ010000000
VERSION     DATIAZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816703"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VLF19_03705"
     CDS             complement(<1..797)
                     /locus_tag="VLF19_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSE92387.1"
                     /translation="MLRVATALLALALAALPASGQPRVTVVAGGLDTPWALAFASDGR
                     IFVTERPGRLRVVRDGRLQADPVATLAVTERGESGLMGLALDPAFAESGRLYVCYTTE
                     KGRRLVNRVVALVLRDGRAREERVLVDDIPGASVHDGCRLKFGPDGKLYATTGDAGES
                     RLAQQSGSLAGKILRLDADGRVPADNPLAGSPVWSLGHRNPQGLAWDDRGRLYAAEHG
                     PSGLPCCHDEVNLILPGRNYGWPEVYGKGGDARFVDPIVESGLGTWAP"
     gene            complement(996..2105)
                     /locus_tag="VLF19_03710"
     CDS             complement(996..2105)
                     /locus_tag="VLF19_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HSE92388.1"
                     /translation="MRVAVLGGGSGGHAAAADLTLGGHAVRFWRRSDEGLGELRAAGG
                     ITLEADGRRGKAEPALVTADLGEAVAGADAIVVPLPAPTHEDLARRLAPHLTERQVVL
                     LTPGTFGSYVMARAVERAGGRLPFAFAETGTLPYLARTTGPAAVKAPVRAANLPAGVL
                     PASRSKETLARVAELFSAARPCADVLDAALTNAGAVIHPPLVLVNAAAIDRGRFDIHA
                     AGTTSSARRLIDAVDAERVAARQGWGYAAPHYELATYYDEARAAEGLYGTGARAKVVQ
                     SGLWSETITFEHRYVQEDAALGLSLLESAARTVSVDSPATSGLLLVFGVLLGRTLAGR
                     GRDLAHLGLGDLLLREVRALLYDGWQSPYWGRVLR"
     gene            complement(2178..3371)
                     /locus_tag="VLF19_03715"
     CDS             complement(2178..3371)
                     /locus_tag="VLF19_03715"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92389.1"
                     /translation="MSLLAGVRVLDLSRMLAGPYGSMLLADMGAEVVKIEDPDGGDPM
                     RVMGPPFLDGGESAYFLAINRNKKSVALDLTRPEGREVFLDLARVADVVWDNFRPGVL
                     ERLGCGTAILAATNPRLVVCSISAYGQDGPYRDWPAFDLALQAMGGAMSLTGESGGRP
                     ARMGLPMGDLAGGMFGALAVAAALFRRQATGAGALIDLSLLDCQVSLLTYMAQYFWTD
                     GRVPGPQGSGHASVVPYQALATRDGHLVVAVFAEKFWGGFCRAVERPEWEHDPRFATN
                     RHRVANRAVLTPLIEALFTSRATDDWLGRLHAEGVPATPILGVDRVLADPQVRQRRMV
                     VEMPHPRQGTVPTLGTPIKVAGAPDFEATPPPALGEHTETVLRGLLKYPAERVAALRA
                     AGVVK"
     gene            complement(3395..4084)
                     /locus_tag="VLF19_03720"
     CDS             complement(3395..4084)
                     /locus_tag="VLF19_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HSE92390.1"
                     /translation="MRTILRFTATERTLHWSCAVGYLALLASGLPLMFPALRSFIRDY
                     TPVIGVRLHVACAILWCGATLAVILLGNRRRLAATCRELTALAPGDLAWMLRFPRWIA
                     AGTAERARLDGAVARFNAGQKVNALFTVVTSALLLLTGIALIPLERGPLIAALAGPGG
                     VAPLAWAHRKLTLLVLLPLATHVLLALVFPPTRPSLTGMVRGSVDRAWAAAHHPRWCP
                     HERPRSGDEAA"
     gene            complement(4081..>4427)
                     /locus_tag="VLF19_03725"
     CDS             complement(4081..>4427)
                     /locus_tag="VLF19_03725"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSE92391.1"
                     /translation="LNPLAAEGQVHGGIHMGLGYALSERLCVDRGQVLTQTFMDYALL
                     RAADMPDIVVRLVESVDAEGPFGAKGLGESGVIPTAAAVANAMKAALDVRFTELPITP
                     AAVRAALEAGAS"
BASE COUNT          636 a         1623 c         1636 g          532 t
ORIGIN      
        1 ggcgcccagg tgccgaggcc tgactccacg atcggatcga cgaagcgcgc gtcaccgccc
       61 ttgccgtaga cctcgggcca gccgtagttg cgtcccggca ggatcaggtt cacctcgtcg
      121 tgacagcacg gcaggccgga cggcccatgc tcggcggcgt agagccggcc gcggtcgtcc
      181 cacgcgagcc cctgcgggtt gcggtggccg agggaccaga ccggcgagcc cgcgagcggg
      241 ttgtcggccg gcacgcggcc gtcggcgtcg agccggagga tcttgcccgc gagcgatccg
      301 ctctgctggg cgagccggct ctcgcccgca tcgccggtgg tcgcgtagag cttgccgtcc
      361 ggcccgaact tgaggcggca gccgtcgtgg accgaggcgc cggggatgtc gtcgacgagc
      421 acgcgctcct cgcgcgcccg cccgtcgcgc aggacgagcg cgaccacgcg gttgacgagc
      481 cgccgtccct tctcggtcgt gtagcacacg tagagccggc cactctcggc gaaggccggg
      541 tcgagcgcca gccccatgag gccgctctcg ccgcgctcgg tgacggcgag ggtcgccacc
      601 gggtcggcct ggagccggcc gtcccgcacg acgcgcaggc ggccggggcg ctcggtgacg
      661 aagatccgcc cgtccgacgc gaatgccagg gcccacggcg tgtcgagccc gcccgcgacg
      721 acggtcacac gaggttgtcc cgaggccggc agcgccgcga gggccagcgc gagcagcgcc
      781 gtcgccacgc gcagcatggt cacgagcgga gtgtagcgtc gctaccgcgg cgcggcaaac
      841 ggagtgtgcc cggcgccgtc gacccaccgg aacccaccgc gcaggaatca ccgatacggg
      901 gaagcgcccg gcggagatca cggtgcgggc gccggcgtcg ccggcttaat cctctccgga
      961 aggtctccga ggccgtcgag gccacgcccg attactcagc ggagtacgcg gccccagtac
     1021 ggcgactgcc agccgtcgta caggagcgcg cgcacctcgc ggagcagcag gtcgccaaga
     1081 ccgaggtggg cgagatcgcg tccgcgaccg gccagcgtcc ggccgagcag cacaccgaac
     1141 acgagcagca ggcccgaggt cgccggcgag tcgacgctca ccgtacgcgc ggcggactcc
     1201 agcagcgaca ggccgagcgc cgcgtcttcc tgcacgtacc ggtgctcgaa tgtgatggtc
     1261 tcgctccaga gaccgctctg cacgaccttc gcccgggcgc cggtgccgta gaggccctcg
     1321 gcggcgcgcg cctcatcgta gtaggttgcc agctcgtagt gcggcgccgc atatccccag
     1381 ccctgccgcg cggccacgcg ctcggcgtcc acggcgtcga tgagccgccg cgcactcgac
     1441 gtggtgccgg cggcgtggat gtcgaagcgc ccccggtcga tggccgcggc attgacgagc
     1501 acgagcggtg ggtggatcac ggcgccggca ttggtcagcg cggcgtcgag gacgtcggcg
     1561 cagggccgcg ccgcggagaa cagctccgcc acccgcgcga gcgtctcctt cgagcgcgag
     1621 gcgggtaaga cacccgccgg caggttcgcc gcgcgtaccg gcgccttcac cgccgccggt
     1681 ccggtcgtgc gcgcgagata cggcagcgtc cccgtctcgg cgaaggcgaa cgggagccgg
     1741 ccgccagcgc gctcgaccgc ccgcgccatc acgtagctgc cgaacgtgcc cggcgtgagc
     1801 aggacgacct ggcgctcggt gaggtggggt gccaggcgcc gcgcgagatc ctcgtgcgtc
     1861 ggcgccggga gcgggacgac gatcgcgtcg gcgccggcga ccgcctcgcc gaggtccgcc
     1921 gtcaccagcg cgggctccgc cttgccccgg cggccgtcgg cctccagcgt gatcccgccg
     1981 gcggcgcgca gctcgccgag cccctcgtcc gaacgccgcc agaaccggac ggcgtgcccg
     2041 ccgagcgtca ggtcggccgc agccgcatgt ccaccactgc caccgccgag cactgccacc
     2101 cgcattcgcg tgatctcctc cctgggggat gatcgatcga tcagtgacac ggggccccga
     2161 tcggcatggg ggccgggtta tttcaccacc ccggccgcgc gcagtgcggc cacgcgctcc
     2221 gcagggtact tcaggagccc gcgcaggacg gtctccgtgt gctcgccgag cgccgggggc
     2281 ggcgtcgcct cgaagtcggg ggcgccggcc accttgatcg gcgtgccgag cgtcggcacc
     2341 gtcccctgtc gcgggtgcgg catctccacc accatgcggc gctgccgcac ctggggatcg
     2401 gccagcacgc ggtccactcc gaggatcggc gtggccggca cgccctcggc gtgcagccgc
     2461 ccgagccagt cgtcggtcgc gcgcgacgtg aacagcgcct cgatcagcgg cgtcaggacc
     2521 gcgcggttgg ccacgcggtg tcggttcgtg gcgaaacgcg ggtcgtgttc ccactccggc
     2581 cgctcgacgg cccggcagaa gccgccccag aacttctcgg cgaacacggc gacgaccagg
     2641 tgcccgtcgc gcgtcgccag cgcctgatag ggaacgacgg aggcgtgccc cgagccctgc
     2701 ggtcccggca cgcggccgtc ggtccagaag tactgggcca tgtacgtgag cagcgagacc
     2761 tggcagtcga gcagcgagag gtcgatgagc gcgcccgcgc ccgtcgcctg gcggcggaac
     2821 agcgcggccg cgaccgccag ggcgccgaac atgcctccgg cgaggtcgcc catcggcagc
     2881 cccatgcgcg cggggcgacc accggactcg cccgtgaggc tcatggcgcc ccccatggcc
     2941 tgcagcgcga ggtcgaacgc gggccagtcg cggtatggtc cgtcctgccc gtacgccgag
     3001 atcgagcaga cgacgagccg gggattggtc gcggccagga tcgcggtccc gcatccgagc
     3061 cgctcgagca cgccggggcg gaagttgtcc cacacgacgt cggcgacgcg cgcgagatcg
     3121 aggaagacct cgcgcccctc cgggcgcgtc agatcgaggg cgacggattt cttgttgcgg
     3181 ttgatcgcca ggaagtacgc gctctcgccg ccgtcgagga acggtggtcc catgacgcgc
     3241 atcgggtcgc caccgtcagg atcctcgatc ttgacgacct cggcgcccat gtcggcgagc
     3301 agcatggagc cgtagggccc ggccagcatc cgggagaggt ccagcacgcg gacgcccgcg
     3361 aggagggaca tggaacgctg ctcgcctgcg cgactcatgc cgcctcgtcc cccgagcgtg
     3421 gccgctcgtg ggggcaccag cgcggatggt gcgccgccgc ccaggcccgg tcgacgctgc
     3481 cacggaccat gccggtcaac gacgggcgcg tcggaggaaa gacgagggcg agcaagacgt
     3541 gggtggcgag cggcagcagc acgagcagcg tgagctttcg gtgggcccac gccagcggag
     3601 cgacgccccc cgggcccgcc agcgcggcga tcagcggccc ccgctcgagg gggatcagcg
     3661 cgatgccggt caggagcagg agggcggagg tgacgacggt gaagagcgcg ttgaccttct
     3721 ggcccgcgtt gaaccgtgcc acggcgccgt cgaggcgcgc gcgctccgcc gtcccggccg
     3781 cgatccaccg cggaaacctg agcatccagg ccagatcgcc gggcgcgagc gccgtgagct
     3841 cccggcacgt cgcggccagc cggcgccggt tgccgagcag gatgacggcc agcgtcgctc
     3901 cgcaccagag gatggcgcag gcgacgtgta gccggacgcc gatgaccggc gtgtagtccc
     3961 ggatgaagct gcgcagtgcc gggaacatca gcggcaaccc gctggctagc agcgcgaggt
     4021 aaccgaccgc gcaggaccag tgcagggtgc gctcggtcgc ggtgaatctc aggatcgtcc
     4081 tcatgacgcg cccgcctcca gcgcggcgcg gacggcggcc ggagtgatcg gcagctcggt
     4141 gaagcgcacg tcgagcgcgg ccttcatcgc gttggccacg gccgcggcgg tcgggatgac
     4201 gccggactcc ccgagcccct tggcgccgaa gggcccctcg gcgtcgacgc tctccacgag
     4261 gcgcacgacg atgtcgggca tatcggcggc gcgcagcagc gcgtagtcca tgaacgtctg
     4321 cgtcagcacc tgcccgcggt ccacgcacag gcgctcgctc agggcgtagc ccaggcccat
     4381 gtggatgccg ccgtgcacct gaccctcggc ggcgagcggg ttcagcg
//
LOCUS       DATIAZ010000109         5236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_290375, whole genome shotgun sequence.
ACCESSION   DATIAZ010000109 DATIAZ010000000
VERSION     DATIAZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290375"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VLF19_03730"
     CDS             complement(<1..992)
                     /locus_tag="VLF19_03730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005240698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92392.1"
                     /translation="MSTVPRPRIGVSVMPLETRREFLTGLATAADRRGYDGFFLPEAW
                     GYDTTVLLADAAARTRRITLGTGILGVWSRSAATIAMAAATLHAVSGGRFVLGLGAST
                     AQLVEGLHDVPFRAPGARMRRTLTQVRALLRGDRVPLAETTGARALKLNVPPVPDLPI
                     YLAALADESIRLAGEHADGWLPFLYPLSQLRNGERLLREGAARAGDPHRALAILPSVP
                     TAVADDPRTAREGAAWFVAFYLTTMGPLYRASLARQGFDKEVAAVLAANAPKFTGAVP
                     PEADRLLEELTVFGTPEEARARLARWHAAGAVMPGLLLRPHLTPEELELTLDAFR"
     gene            1092..2159
                     /locus_tag="VLF19_03735"
     CDS             1092..2159
                     /locus_tag="VLF19_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSE92393.1"
                     /translation="MIKPYTAVGLIPTVRGIRKRKDIKINLEHLSHLVKAASWLSSLD
                     LPVRLIAFPEGALQGFNDEVLDLDHAQFAREGAIDIPGEETEFLGTIAKEYDAFVMAQ
                     AKARHPDLKDRFFNVGFVINPRGKVILQHYKVSPLFPVEHSVCPHDVYDWWVEKYGRT
                     LDAFWPVVDTPIGRLGIMMANEGSYPENARALAMNGAEVVYRASYPHPATGNEIFEIQ
                     SRARALDNNMYLVAPNMGTYYLFPEETTPIDTFGGRSFIVNYKGQIVGRQEYGAGSTY
                     VGGVIDIEALRDHRARAQWDNWMKDLRTELYQIVYEKPIYPKNLYLTRAPMKHAEYRD
                     KVIKKQIRLMHDRGIWAKPDR"
     gene            complement(2185..2361)
                     /locus_tag="VLF19_03740"
     CDS             complement(2185..2361)
                     /locus_tag="VLF19_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92394.1"
                     /translation="MTTRRRRLGLALAGAVVIAGGLGVGVVELMRLPKGSVWVVVAAT
                     LGLVALIRALTTRR"
     gene            complement(2358..3182)
                     /locus_tag="VLF19_03745"
     CDS             complement(2358..3182)
                     /locus_tag="VLF19_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSE92395.1"
                     /translation="MLAAQPRLGALLAALGLVDAHMSVERLGLERLFVDDGEPPLEAL
                     RAASPVVCWFAARDPVFVRRLREAVPEAVVASPIGDGSRPVWEHLLATVGSARGPWRD
                     PLAVPAPLAASGHAALRGAGWDGATPLVLVHAGAGGLGKRWPVDGFARALHAVSARRR
                     LALALVQGPADADATAALAARLPDAAVLPEMSLGDLAGALGHAGAWLGNDSGIGHLAA
                     AMGVPTLVLFKAANLAWRSWSASARTVTVDPGALKEEDVSAVVAGLEALLGGERGP"
     gene            complement(3289..4047)
                     /locus_tag="VLF19_03750"
     CDS             complement(3289..4047)
                     /locus_tag="VLF19_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSE92396.1"
                     /translation="MTERALAAAVEAARAAGEIAMKYYRGGFDVTLKADLTPVTQADR
                     EAEQAIVEVLARAFPSHGFLGEEFGERGPREPRWIIDPIDGTKNFVRRIPFWSTLIGL
                     EEEGEITVGVVHNPATGELYTARRGAGARLNGAPLRVSDIGELGRAQLLHAGLRLLRE
                     AGCWDGFVRLVDATDRQRGFGDYMGYGFVAEGKAEIYLETDLKPWDLAPCKLLVEEAG
                     GRFTDLHGRPTIYSGSALATNGRVHDAVLALLSP"
     gene            complement(4053..4454)
                     /locus_tag="VLF19_03755"
     CDS             complement(4053..4454)
                     /locus_tag="VLF19_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSE92397.1"
                     /translation="MLDDAELRERTRRTARTLFHSLTAGTGFEAWKRFDRGLARQLSM
                     FFTGVLYSREVISQKQRELCAVASLTVLHRPHELRAHVHAALNVGATRQEVAEVIFQQ
                     VTYGGMPVVVDALDVYARVLEERGEPFPAEG"
     gene            complement(4532..>5236)
                     /locus_tag="VLF19_03760"
     CDS             complement(4532..>5236)
                     /locus_tag="VLF19_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE92398.1"
                     /translation="FAFVHGMSHGAWCWRPVTERLEGRGHRVIAVDLPGHGPRAHEWR
                     HASIGAYARAVADALALAGFSRAVVVGHSMAGAVIPKVVELTPARVAHLVFLAAVVPA
                     RRGRLADHFPPSVRALFRGLARAAGGVVQYPAQVEHARWMTDLCPGDPRVVDALARLT
                     PQPAAPWEERVDYGRFYATNVPRTYVRCLGDAAVTPERAAQYAARLGVVPIDLDTAHG
                     PMLSAPDELVRILERV"
BASE COUNT          803 a         1917 c         1888 g          628 t
ORIGIN      
        1 cggaacgcgt ccagcgtgag ctcgagctct tcgggcgtga ggtgcggccg gaggagcagg
       61 ccgggcatga cggcgccggc agcgtgccat cgggcgagac gggcgcgggc ctcctccggc
      121 gtgccgaaca ccgtcagctc ctcgaggagc cggtccgcct cgggcggcac cgcgccggtg
      181 aacttcggcg cgttcgcggc gagcacggcc gccacctcct tgtcgaaccc ctggcgcgcg
      241 agcgaggcac ggtagagcgg ccccatcgtc gtgagataga aggcgacgaa ccacgccgca
      301 ccctccctgg ccgtccgggg atcgtcggcg accgccgtcg gcaccgaggg aaggatcgcg
      361 agcgcccggt ggggatcgcc ggcgcgtgcc gcgccctcgc gaagcagccg ctcgccgttc
      421 cggagctgac tgagcggata gaggaacggc agccacccgt cggcgtgctc gcccgccagc
      481 cggatcgact cgtcggccag cgcggccagg tagatcggca ggtctggcac gggcggaacg
      541 ttgagcttca gcgcgcgtgc ccccgtcgtc tccgccagcg ggacccgatc gccgcgcagc
      601 agcgcccgca cctgcgtgag cgtgcgccgc atgcgcgcgc ccggcgcccg gaagggcacg
      661 tcgtgcagcc cctcgacgag ctgtgccgtg ctcgcaccga gcccgagcac gaagcggccg
      721 cccgacaccg catgcagcgt ggcggcggcc atggcgatcg tcgccgcgct ccggctccac
      781 acgccgagaa tcccggtgcc gagcgtgatc cgccgcgtgc gcgcggcggc gtcggcgagc
      841 aggaccgtgg tgtcgtagcc ccaggcctcc gggaggaaga agccgtcgta gccgcgtcga
      901 tcggcggcgg tcgccaggcc ggtgaggaac tcgcgacggg tctccagcgg catgacggag
      961 acaccgatgc gcggcctggg cacggtgctc atgaaggctc ctcgcgagtc cacgtctttt
     1021 cggagccgcg gacctgtgct agttgtcgag gcgcagcgga actctgcggg actctaacag
     1081 aggagccgtc gatgatcaaa ccgtacaccg ccgtcggcct gatcccgacc gtccgtggca
     1141 tccgcaagcg caaggacatc aagatcaacc tcgagcatct ctcgcacctc gtgaaggcgg
     1201 cgtcgtggct gtcgagcctc gacctgcccg tgcggctcat cgccttcccc gagggcgcgc
     1261 tgcagggctt caacgacgag gtcctggacc tcgaccacgc ccagttcgcc cgcgagggcg
     1321 cgatcgacat tcccggcgag gagacggagt tcctggggac catcgcgaag gagtacgacg
     1381 cgttcgtcat ggcgcaggcc aaggcgcgcc atcccgacct caaggaccgc ttcttcaacg
     1441 tcggcttcgt catcaacccc cgcggcaagg tgatcctcca gcactacaag gtctcgccgc
     1501 tgtttcccgt cgagcactcg gtctgcccgc acgacgtcta cgactggtgg gtggagaagt
     1561 acgggcgcac gctggacgca ttctggcccg tcgtggacac cccgatcggc cggctgggca
     1621 tcatgatggc caacgagggc tcctatcccg agaacgcccg cgcgctggcc atgaacggcg
     1681 ccgaggtggt ctatcgcgcc tcctacccgc acccggccac cggcaacgag atcttcgaga
     1741 tccagagccg cgctcgagca ctggacaaca acatgtacct ggtcgcgccg aacatgggca
     1801 cctactacct gttccccgag gagacgacgc ccatcgatac cttcggcggg cggtccttca
     1861 tcgtcaacta caagggccag atcgtcgggc gccaggagta cggtgccggc tcgacctacg
     1921 tcggcggcgt gatcgacatc gaggccctgc gcgaccaccg cgcgcgggcc cagtgggaca
     1981 actggatgaa ggatctccgg accgagctct accagatcgt ctacgagaag ccgatctacc
     2041 cgaagaacct ctacctcacc cgcgcgccca tgaagcacgc cgagtaccgc gacaaggtca
     2101 tcaagaagca gatccggctc atgcacgacc gaggcatctg ggcgaagccc gatcgttgag
     2161 ggcggccgcg cgtcgtcccg ttcgtcagcg ccgggtggtc agcgccctga tcagcgcgac
     2221 cagcccgagg gtggcggcga cgacgaccca gaccgagccc ttcggcagcc gcatcagctc
     2281 cacgacgccg acgccgagcc cacccgcgat cacgacggca cccgcgaggg cgaggccgag
     2341 tcgccgccga cgcgtggtca tggaccgcgc tcgccgccca gcagcgcctc caggccggcg
     2401 acgaccgcgc tcacgtcctc ctccttcagc gcgccggggt cgacggtcac cgtccgggcg
     2461 gacgcgctcc acgatcgcca ggcgagattc gccgccttga acagcaccag cgtcggcacc
     2521 cccatggcgg cggcgaggtg accgatgccg gagtcgttgc ccagccaggc gccggcgtga
     2581 ccgagggcgc cggcgagatc gccgagcgac atttccggca gcacggcggc gtccggcagc
     2641 cgcgcggcca gcgccgccgt cgcgtcggcg tccgctggcc cctggacgag cgcgagagcg
     2701 agccgccgtc gggcgctgac ggcatggagc gcccgagcga acccgtccac gggccagcgc
     2761 ttgccgagac cgccggcgcc ggcgtggacg aggacgagcg gcgtcgcgcc gtcccagccc
     2821 gcgccgcgca gggccgcgtg ccccgacgcg gcgagcggcg cgggcacggc gagcgggtcg
     2881 cgccacgggc cccgggcgga gccgaccgtg gccagcaggt gctcccacac cggccgcgag
     2941 ccgtcaccga tcggcgaggc gaccaccgcc tccgggacgg cctcgcgcag ccgccgcacg
     3001 aagacgggat cgcgcgcggc gaaccagcag acgaccggcg acgcggcgcg cagcgcctcg
     3061 aggggcggct cgccgtcgtc gacgaagagc cgctcgaggc ccaggcgctc gaccgacatg
     3121 tgcgcatcca cgaggcccag cgcggccagc agcgcgccca gccggggctg ggcggcgagc
     3181 acgagggcgt cgtgcgggcg ggccgcgcgg agcgcgcgca gcgccggtac gccgaggagg
     3241 acgtcaccga gcgcgccagg gtggatgacg agcgtcgccg acaggctcct agggagagag
     3301 aagcgccagc acggcgtcgt ggacgcgccc gttcgtggcc agcgcgctcc cggaatagat
     3361 cgtcggccgc ccgtggagat cggtgaagcg gccacccgcc tcctcgacga gcagcttgca
     3421 gggggcaaga tcccacggct tgagatcggt ctcgagatag atctcggcct tgccctcggc
     3481 cacgaagccg tagcccatgt agtcaccgaa gccgcgctgc cggtcggtgg cgtccacgag
     3541 gcgcacgaag ccgtcccagc agccggcctc gcggagcagg cgcagcccgg cgtgcaggag
     3601 ctgggcgcgg ccgagctcgc cgatgtccga gacgcggagc ggagcgccgt tgaggcgggc
     3661 gcccgcgccc cgccgcgcgg tgtagagctc gccggtcgcg gggttgtgga cgacgccgac
     3721 ggtgatctct ccctcctcct cgagcccgat cagcgtcgac cagaacggaa tgcggcgcac
     3781 gaagttcttg gtgccgtcga tgggatcgat gatccagcgc ggctcgcgcg gaccgcgctc
     3841 gccgaattcc tcgccgagga agccgtgcga ggggaaggcg cgggccagca cctcgacgat
     3901 cgcctgctcc gcctcgcggt cggcctgcgt gaccggcgtg aggtcggcct tgagcgtgac
     3961 gtcgaacccg ccacggtagt acttcatggc gatctcgccg gcggcgcggg cggcttcgac
     4021 ggctgccgcc agcgcacgct cggtcacggc tctcatccct ccgcggggaa cggctcgccg
     4081 cgctcctcga gcacccgggc gtagacgtcg agggcgtcga cgacgacggg catccccccg
     4141 taggtcacct gctggaagat cacctcggcg acctcctggc gcgtcgcgcc cacgttgagc
     4201 gcggcgtgca cgtgcgcgcg cagctcgtgc gggcggtgca gcacggtgag cgaggccacc
     4261 gcgcacagct cgcgctgctt ctgcgagatg acctcgcggc tgtagagcac gcccgtgaag
     4321 aacatcgaga gctggcgcgc cagcccgcgg tcgaagcgct tccacgcctc gaacccggtg
     4381 ccggccgtca gcgagtggaa cagcgtgcga gccgtgcgcc tcgtgcgctc ccgcagctcg
     4441 gcgtcgtcga gcacggcagt ccgctcgccg gcgcgacggg cggggtccgg cccgctcggg
     4501 tggcccggcg aggaccccgg attcggcgtg ctcacacccg ctccaggatg cgcacgagct
     4561 cgtcgggggc cgagagcatc gggccgtgcg ccgtgtcgag gtcgatcggg accaccccga
     4621 gccgggcggc gtactgtgcg gcgcgctccg gcgtcaccgc ggcgtcgccc aggcaacgca
     4681 cgtaggtgcg tggcacgttc gtggcgtaga accggccgta gtccacgcgc tcctcccagg
     4741 gcgccgccgg ctggggcgtg agacgcgcca gcgcgtccac cacgcgaggg tcgccggggc
     4801 acagatccgt catccaccgc gcgtgctcga cctgcgcggg atactgcacg acgccgcccg
     4861 ccgcgcgggc caggccgcgg aacagggcac gaacggacgg tggaaaatgg tcggcgaggc
     4921 gcccgcggcg ggccggtacg acggcggcga ggaacacgag gtgggccacc cgcgccggcg
     4981 tcagctcgac gaccttcggg atgacggcgc ccgccatgga gtggcccacc accaccgcgc
     5041 gagagaagcc ggccagcgcc agggcgtcgg cgaccgcgcg cgcgtaggcg ccgatcgacg
     5101 cgtgccgcca ctcgtgagcg cgcggcccgt ggccgggcaa gtccacggcg atcacgcggt
     5161 ggccccgccc ttcgagacgc tcggtgaccg gtcgccagca ccaggcgccg tgactcatgc
     5221 cgtgaacgaa cgcgaa
//
LOCUS       DATIAZ010000110         6882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1738179, whole genome shotgun sequence.
ACCESSION   DATIAZ010000110 DATIAZ010000000
VERSION     DATIAZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738179"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VLF19_03765"
     CDS             <1..952
                     /locus_tag="VLF19_03765"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HSE92399.1"
                     /translation="ECEWKVFDWSCERFTKTGRMMPEDGLDQLRAFEAIYLGAVGFPG
                     VPDHVSLWGLLIPIRRGFRQYVNLRPVRLLRGVRSPLRDRGPADIDMLIVRENNEGEY
                     SEIGGRLYKDSAEELAVQQAVFTRRGCDRIMRFAFERARQRRRHVTSATKSNGIIHSM
                     PYWDERFAAMAQQYPDVKTAQYHIDILTAHFVQHPDWFDVVVGSNLFGDILSDLGPAI
                     AGSIGIAPGGNINPEKDYPSMFEPVHGSAPDIAGKGIANPVAQIWTGAMLIEHLGHVD
                     AAGAIVAAIEKVVEEGRAVTRDLGGKASTREAGEAIAALI"
     gene            1030..1215
                     /locus_tag="VLF19_03770"
     CDS             1030..1215
                     /locus_tag="VLF19_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92400.1"
                     /translation="MQVHRPPPAPAWGSDGAEILMPGERAHRTYEQRTREGIPISHPV
                     HDALARVAGRLGVPMLG"
     gene            complement(1227..2336)
                     /locus_tag="VLF19_03775"
     CDS             complement(1227..2336)
                     /locus_tag="VLF19_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92401.1"
                     /translation="MSRRGWLGGGGAVALGLVVVTGAAWTSGLVGAQEPAAPAPKLEV
                     DPFWPKPLPNNWLMGQASGVAVDRRDRVWVIQRPKSLTEDERGATLAPPRSRCCVPAP
                     PVMEFDGDGTLVQAWGGPGGGHAWPGSEHGIFVDHQDHVWVAGNGPKDGQILKFTRDG
                     RFLMAIGAPGVVGDDADTGHLNRPAGMVVDPKTNELFVADGYGNHRVIVFDAQTGAYK
                     RHWGANGRPPGDTSVKGFGNPVHCVRLARDGLLYVCDRRNNRIQVFRRDGTFVKEFVV
                     APDTRGNGSTWDADFSPDQAQTFLFNADGENNHVWTLLRDSGRILGTFGRSGRGAGQF
                     HWIHNLAVDSKGSIYTTEVDTGKRAQKFVAKGATH"
     gene            complement(2406..3968)
                     /locus_tag="VLF19_03780"
     CDS             complement(2406..3968)
                     /locus_tag="VLF19_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92402.1"
                     /translation="MHRRSRPLGVPARLRLVAHLLLFAVVLVVVPRADAAPEGTVTWA
                     IHFTLAPKWLDPAETEAAITPFKVLYPIHDALVKLMPGGMTPSLAESWTTSPNGLTYD
                     FVLRGGAKFHNGEPVTAEDVKFSFDRYKGAGAKLLKDRVKEVQILDARRVRFVLKEAW
                     PDFMTFYGTTATGAAWIVPKKYVEKVGDEGFKKSPVGAGPYRFVSVNPGVDMVLEAFE
                     GYWRKVPSVKRLVFKSVPDETTRAAALKRGEVDIAYFLNGPIAADIRRTPGLKLMAVR
                     TNASFFLDFLDQWDPKSPWHDRRVRLAASLAVDRKAINEAEMLGFAGLTGNIVPRHME
                     FALPIDPHPHDVKRARQLLAEAGYPNGFDGGDLTPTPPYNSMAEAIGSNLGAIGIRTR
                     IRTMERVTYLTSWREKKLRGVLFTALGAGGNAGTRIEALATRGGLYAYGVLPEVEDLF
                     QRQVRELDRKKREELLHEIQRILHDRVVFAPIWENALISGVGPRVEEPALGLIPAFPY
                     SAPLEELRLKRP"
     gene            4144..4938
                     /locus_tag="VLF19_03785"
     CDS             4144..4938
                     /locus_tag="VLF19_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSE92403.1"
                     /translation="MSEAPKLLLQKDGPIGWIMFNQPEKRNAVSQEMWQLIPDYVRDL
                     ATDDAIRVVILRGAGDQAFVAGADISQFKERRRNMDDEAEYRRISGRGQEALATLAKP
                     LLAMIHGFCVGGGVGIAVTCDLRIAADDARFGVPAARLGLGYHYHGMEKLMALVGPAY
                     TKEIFFTARTDFSAQDARRMGLVNQVVPKAELETFTREYALKIAGNAPLTLRSAKTTV
                     EQLLRPAGQRDLALLDKLVADCFNSEDYQEGVRAFSEKRRPQFKGR"
     gene            4968..5933
                     /locus_tag="VLF19_03790"
     CDS             4968..5933
                     /locus_tag="VLF19_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92404.1"
                     /translation="MVQADRRLAGQVRRLAGQRMVFFAGLPGTGKSLLVHQLAHLAGA
                     GGRRVHLVQWDVARPVFEASPAARRYPVVEGVTHVVVRKAAGLWVRHALAEWSARHPE
                     PEHLLIGETPFVGGRFIELARRRNDRAEALLAAPACRFVIVVPSVDVRRFLEAERERR
                     AANPLHPREREDAPPGVLRELWRELVDVARRLDLEAPVDAGDASAAYDPTVYRRVYET
                     ILHDRHVEVVALDVILPTAGLSVYDFAAAPLDLVPSEADASSFIAEVERRYADPMALE
                     REIARWWQVPGTALTPARTPPARPRPGTTRGGTPPRSRRSSAGPA"
     gene            complement(5836..6621)
                     /locus_tag="VLF19_03795"
     CDS             complement(5836..6621)
                     /locus_tag="VLF19_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HSE92405.1"
                     /translation="MIVRSDPVGGLVLIGQTDHSRFVGQVAAHWGNRDFEPPAPYDSV
                     VRAAAFHDFGWLRYETSPLVDPESGEPYQFLRAPMTEQQLGSYQWALDWMTDIDPYSG
                     LIVSMHRTGLWQGRYGRIKHPGGRYNPTGLGPEIRAFIAHNEAWQVRQRAALDEAEVW
                     TNYTLMQVWDLLGLYFGCQEPYADHAEPVPAGYGTDRDGVRMTMTPEGPRRVAFDPYP
                     FDVRPCRVQLAVRRVPRTTFESAEAFRRAWFQAEVGLEEFELV"
     gene            complement(6646..>6882)
                     /locus_tag="VLF19_03800"
     CDS             complement(6646..>6882)
                     /locus_tag="VLF19_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSE92406.1"
                     /translation="CLRAGDLALVRSGLLHLGNSSLRVLHRLANVRTGEVVATLEQAG
                     VHFDMDARRPTPLPAALRERAATMLLSRPPVVSS"
BASE COUNT         1001 a         2444 c         2427 g         1010 t
ORIGIN      
        1 cgagtgcgag tggaaggtct tcgactggag ctgcgagcgc ttcaccaaga ccggccggat
       61 gatgcccgag gacggcctcg atcagctccg cgccttcgag gccatctacc tcggcgccgt
      121 cggctttccc ggcgtgccgg accacgtgtc gctgtggggc ttgctcatcc ccatccggcg
      181 cgggttccgc cagtacgtga acctccggcc cgtccgcctt ctccgtggcg tgcgctcgcc
      241 gctgcgcgac cgcggccccg ccgacatcga catgctgatc gtgcgcgaga acaacgaggg
      301 cgagtactcg gagatcggcg gccggctcta caaggacagc gccgaggagc tggccgtgca
      361 gcaggccgtc ttcacccggc gcggctgcga ccgcatcatg cggttcgcct tcgagcgcgc
      421 ccgccagcgg cggcggcacg tgacctcggc gaccaagtcg aacggcatca tccactcgat
      481 gccctactgg gacgagcgct tcgcggccat ggcgcagcag taccccgacg tgaagaccgc
      541 gcagtaccac atcgacatcc tcaccgcgca cttcgtgcag cacccggact ggttcgacgt
      601 cgtcgtgggg tccaacctgt tcggcgacat cctctccgac ctggggccgg cgatcgccgg
      661 ctccatcggc atcgcgccgg gcggcaacat caaccccgag aaggactacc cctcgatgtt
      721 cgagcccgtt cacggctcgg cgccggacat cgcgggcaag ggcatcgcca accccgtggc
      781 ccagatctgg accggcgcca tgctgatcga gcacctgggc cacgtcgacg ccgccggcgc
      841 cattgtcgcc gccatcgaaa aggtggtcga ggagggacgg gcggtgacgc gggatctcgg
      901 aggcaaggcc tccacgcgcg aggccggaga ggcgatcgcc gcgctgatct gatcatcgcc
      961 tgcacgctcc ccggcacgca tccggcccgg cgcgcccgcg gaccgaggac cgatcagctc
     1021 ctcctccgcg tgcaggttca ccgtccccca ccggcgccgg cgtggggatc ggacggcgcc
     1081 gagatcctca tgcccggcga gcgcgcccac cgcacgtacg agcagcgtac ccgtgagggg
     1141 attccgatct cccacccggt gcacgacgcg ctcgcgcgcg tcgccgggcg cctcggcgtt
     1201 ccgatgctcg ggtgaccgca gccggctcag tgcgtggcgc ccttggccac gaacttctgc
     1261 gcgcgcttgc ccgtgtcgac ttccgtcgtg tagatgctgc ccttcgagtc gaccgccagg
     1321 ttgtggatcc agtggaactg gcctgcgccg cgcccggacc taccgaacgt cccgaggatc
     1381 cggccggagt cacgcagcag cgtccagacg tggttgttct cgccgtcggc gttgaagagg
     1441 aacgtctggg cctggtcggg cgagaagtcg gcgtcccagg tggagccgtt gccccgggtg
     1501 tcgggcgcca ccacgaactc cttcacgaac gtgccgtccc tgcggaagac ctggatgcgg
     1561 ttgttccggc gatcgcagac gtagagcagg ccgtcacgcg cgaggcggac gcagtgcacg
     1621 gggttgccga accccttgac gctcgtgtcg ccgggcgggc gaccattggc gccccagtgg
     1681 cgcttgtagg cgccggtctg ggcatcgaag acgatgacgc ggtggttgcc atagccgtcg
     1741 gcgacgaaga gctcgttggt cttcgggtcc acgaccatgc cggccggccg gttcaggtgc
     1801 ccggtgtcgg cgtcatcgcc cacgacgccg ggtgcgccga tcgccatcag gaagcggccg
     1861 tcgcgggtga acttgaggat ctggccgtcc ttcgggccgt tgccggcgac ccacacgtga
     1921 tcctggtggt cgacgaagat gccgtgctcg ctgcccggcc atgcatggcc gccgccgggg
     1981 ccaccccacg cctgaacgag ggtgccgtcg ccgtcgaact ccatcaccgg cggcgccggt
     2041 acgcagcacc gcgagcgtgg tggagcgagc gtcgccccgc gctcgtcctc ggtcagtgac
     2101 ttgggccgct ggatcaccca cacgcggtct cgccggtcga cggccacacc tgacgcctgg
     2161 cccatcagcc agttgttcgg cagcggcttc ggccagaacg gatcgacctc gagcttcggc
     2221 gccggcgccg ccggctcctg cgccccaacg agaccgctcg tccatgcggc gcccgtcacc
     2281 accacgagcc cgagggcgac ggctccgcca cccccgagcc aaccgcgtcg gctcatgtcg
     2341 cgcgctcccc caccgtcagc aatcaagccg cgcgccgccg ccccgcccca cgatggccgc
     2401 gatggtcacg gacgcttcag gcgcagctcc tccaggggcg ccgagtaggg gaacgccggg
     2461 atcaggccga gcgccggctc ttccacccgc ggcccgacgc ccgagatgag cgcgttttcc
     2521 cagatcggcg cgaacaccac ccggtcgtgg aggatccgct ggatctcgtg gagcagctcc
     2581 tcgcgcttct tccggtccag ctcccgtacc tgccgctgga agaggtcctc cacctcgggg
     2641 agcacgccgt aggcgtacag cccgccccgc gtcgccaacg cctcgatgcg ggtgccggcg
     2701 ttcccgccgg cgcccagcgc cgtgaagagg actccgcgga gcttcttctc acgccagctc
     2761 gtgaggtacg tcacgcgctc catcgtgcgg atccgcgtgc gaatgccgat ggcgccgagg
     2821 ttgctgccga tcgcctccgc catggagttg tagggcggcg tcggggtgag gtcgccgccg
     2881 tcgaagccgt tcggataccc cgcctcggcg aggagctgcc gggcgcgctt gacgtcgtga
     2941 ggatgcgggt cgatcggcag cgcgaactcc atgtgacggg ggacgatgtt tccggtgagc
     3001 ccggcgaagc ccagcatctc ggcctcgttg atggccttgc ggtcgacggc caggctggcg
     3061 gcgagccgaa cgcgccggtc gtgccagggc gacttcgggt cccactggtc gaggaagtcg
     3121 aggaagaagg acgcgttggt gcggacggcc atgagcttga ggccgggggt gcgcctgatg
     3181 tccgcggcga tggggccgtt caggaagtag gcgatgtcga cctcgccccg cttgagcgcc
     3241 gccgcgcgcg tggtctcgtc ggggacgctc ttgaacacga ggcgcttgac gctcggcacc
     3301 ttgcgccagt agccctcgaa ggcctccagc accatgtcca cgccggggtt gacgctcacg
     3361 aaccggtagg gaccggcgcc gaccggagac ttcttgaagc cctcgtcgcc gaccttctcc
     3421 acatatttct tcgggacgat ccaggcggca ccggtggccg tcgtgccgta gaacgtcatg
     3481 aagtccggcc aggcctcctt gagcacgaag cgcacccgcc gagcgtcgag gatctgcacc
     3541 tccttcaccc ggtccttgag gagcttggcg cccgcgccct tgtagcggtc gaaggagaac
     3601 ttcacgtcct ccgccgtcac cggctcgccg ttgtggaact tcgcgccgcc gcgcaggacg
     3661 aagtcgtagg tcagcccgtt cgggctcgtc gtccaggact cggcaaggct cggcgtcatg
     3721 ccgccgggca tcagcttcac cagggcgtcg tggatcgggt agaggacctt gaacggcgtg
     3781 atggcggcct cggtctcggc cggatccagc cacttggggg ccagcgtgaa gtgaatggcc
     3841 caggtcaccg ttccctccgg cgcggcatcc gcacgcggca cgaccaccag cacgaccgcg
     3901 aacaggagca ggtgagcgac caaccggagc cgggcgggca cgccgagcgg tcgggaccga
     3961 cggtgcatga gcaaccctcc tcgtggagcg gtggtgagag ccttctaccc cgccgccgtc
     4021 gcgaccgcaa gggtcggcgg ctaccgccgc ttgcctaccg gacgagagcc gcttactctg
     4081 cccgcgggcc cgaggcaaca cgtgggacgc cgggtcgacg ccagcccgag gtgaggagac
     4141 gccatgagcg aggcaccgaa gctgctgctg cagaaggacg ggccgatcgg ctggatcatg
     4201 ttcaatcagc cggagaagcg caacgcggtg agccaggaga tgtggcagct cattccggac
     4261 tacgtccggg acctggccac cgacgacgcg atccgtgtgg tgatcctgcg cggcgccggc
     4321 gaccaggcct tcgtggccgg agccgacatc tcgcagttca aggagcgccg ccgcaacatg
     4381 gacgacgagg cggagtaccg ccggatctcc ggacgcggcc aggaggccct ggcgacgctg
     4441 gcgaaaccgc tgctggccat gatccacggt ttctgcgtgg gcggcggcgt gggcatcgcc
     4501 gtcacctgcg atctgcgcat cgcggccgac gacgcgcgct tcggcgttcc cgcggcgcgc
     4561 ctggggctcg ggtaccacta ccacggcatg gagaagctga tggcgctggt cgggcccgcg
     4621 tacacgaagg agatcttctt caccgcccgc accgacttca gcgcgcagga cgcgcgccgc
     4681 atgggtctcg tgaaccaggt cgtgccgaag gccgagctgg agacgttcac gcgcgagtac
     4741 gcgctgaaga tcgcgggcaa cgctccgctc acgctgcgct cggccaagac cacggtggaa
     4801 cagctcctgc gccccgcggg gcagcgcgat ctcgccctcc tcgacaagct cgtcgccgac
     4861 tgcttcaaca gcgaggacta ccaggagggc gtccgcgctt tctcggagaa gcgccgcccg
     4921 cagttcaagg gccgctgacg agctcccggc ccggagacgc ccgtctcgtg gtccaggccg
     4981 accgccggct cgccgggcag gtccgtcgcc tggccgggca gcggatggtc ttcttcgccg
     5041 gtcttcccgg cacgggcaag agcctcctcg tccaccagct cgcgcacctg gccggcgccg
     5101 gcggccggcg ggtgcacctg gtccagtggg acgtggcgcg cccggtgttc gaggcgagcc
     5161 cggccgcgcg gcgctatccg gtggtggagg gggtcaccca cgtcgtcgtc aggaaggcgg
     5221 ccgggctctg ggtgcggcat gctctcgccg agtggagcgc gcgtcatcca gagcccgagc
     5281 acctgctgat cggcgagacg ccgttcgtcg gcggccggtt catcgagctg gcccggcgcc
     5341 gcaacgaccg cgccgaggcg ctgctggccg ctccggcgtg ccggttcgtg atcgtggtgc
     5401 cgtcggtcga cgtgcggcgc ttcctcgagg ccgagcgcga gcgccgggcg gcgaatccgc
     5461 tccacccgcg cgagcgcgag gacgcgccgc cgggcgtgct gcgcgagctc tggcgcgagc
     5521 tcgtggacgt ggcgcgccgg ctcgacctcg aggcgccggt cgacgccggc gacgcatcgg
     5581 cagcgtacga ccccacggtc taccgccggg tctacgagac catcctgcac gatcggcacg
     5641 tggaggtcgt cgcgctcgac gtgatcctgc cgacggcggg gctgtcggtg tacgacttcg
     5701 cggcggcccc gctcgacctg gtgccgagcg aggcggacgc ctcgagcttc attgccgagg
     5761 tcgagcgccg gtacgcggac ccgatggcgc tcgagcgcga gatcgcgcgc tggtggcagg
     5821 ttccgggaac ggcccttaca ccagctcgaa ctcctccagc ccgacctcgg cctggaacca
     5881 cgcgcggcgg aacgcctccg cgctctcgaa ggtcgtccgc gggacccgcc tgacggcgag
     5941 ctgcacgcgg cacggccgca cgtcgaaggg gtacgggtcg aaggcgacgc ggcgggggcc
     6001 ctcgggcgtc atcgtcatcc ggaccccgtc gcggtcggtc ccgtacccgg ccgggaccgg
     6061 ctcggcgtgg tccgcgtacg gctcctggca gccgaagtag aggccgagga gatcccacac
     6121 ctgcatgagc gtgtagttcg tccacacctc cgcctcgtcg agcgccgcac gctgccgcac
     6181 ctgccaggcc tcgttgtgag cgatgaaggc gcggatctcg gggccgagcc cggtggggtt
     6241 gtagcgcccg ccggggtgct tgatcctccc gtaccgtccc tgccagaggc cggtgcgatg
     6301 catgctgacg atgaggccgg agtacgggtc gatgtcggtc atccagtcga gcgcccactg
     6361 gtaagagccg agctgctgct cggtcatcgg cgcccggagg aactgatagg gctcgccgct
     6421 ctcggggtcg acgagcgggc tcgtctcgta tcgcagccag ccgaaatcgt ggaacgccgc
     6481 cgcgcggacc acgctgtcgt agggcgcggg tggctcgaag tcgcgattgc cccagtgggc
     6541 cgcgacctgg ccgacgaagc gggagtggtc ggtctgaccg atgagaacga gcccgccgac
     6601 aggatcgctc cggacgatca tgggcgcctc ctcgcgatca ttcgctcacg atgatactac
     6661 cggaggacgc gacagcagca tggtcgcggc gcgctcgcgg agcgccgccg gcagcggggt
     6721 cggtcggcgc gcgtccatgt cgaagtgcac gccggcctgc tcgagggtcg ccacgacctc
     6781 gccggtccgg acgttcgcca gccggtgcag gacgcgcagc gaggagttgc ccaggtgcag
     6841 cagcccgctg cgaacgagcg cgagatcacc cgcgcgcagg ca
//
LOCUS       DATIAZ010000111         4903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_948173, whole genome shotgun sequence.
ACCESSION   DATIAZ010000111 DATIAZ010000000
VERSION     DATIAZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948173"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VLF19_03805"
     CDS             <1..309
                     /locus_tag="VLF19_03805"
                     /inference="COORDINATES: protein motif:HMM:NF036395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase family protein"
                     /protein_id="HSE92407.1"
                     /translation="LNDELGAWPGVKITPMFGRWGYFVGRQLFACFPLREKDRDLWVR
                     LGTDDQARALRDARVRPHRRFARRGWVELNVEEPADLVLALRWLRRAHAAAARGLEED
                     "
     gene            complement(323..607)
                     /locus_tag="VLF19_03810"
     CDS             complement(323..607)
                     /locus_tag="VLF19_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92408.1"
                     /translation="MTVARICTIGLVALVLATACAKPIPARVVKDVQAVQQCQYVGLV
                     SDTDYVDLARKAGEVGGTHALIIGEKPDRGPFGIEIGKAMVAEVFRCPSP"
     gene            complement(688..1953)
                     /locus_tag="VLF19_03815"
     CDS             complement(688..1953)
                     /locus_tag="VLF19_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92409.1"
                     /translation="MLERLRRLHPFKTPDAQRLALLFGTVYFAQGMWYLPNQTITIVL
                     KERGLSAGQVADFFLVATVPWLIKPVYGLISDFVPLWGRRRKSYYLLTTSLAIAAGTA
                     LASWAGHPYRAMMVMFTIMGLGLAFTDVLTDALMVENGRPRGLTGAFQSVQWAAITAA
                     SVAVGLLGGQLAGARNLALAFALAACLPVVSLVMGLRFIDERPGVVDREAVRETWRAI
                     RTAMTDRSVWVVAGFIFFYTFSPSFGPAFLYYQTDVLKFSQEFIGALDSMYYGAAMAG
                     AIVYAPLSRRMPLKRLIVAAIGAALVSTLAYLFYRDRISAIGIQVLFGVVAMITQLAF
                     LDLAAKACPRRVEATFFALLMSVYNAGVQLSQNVGARLYDGFGYTPLVLISAALTVLA
                     WPLVPLVRIDRIEAKAWAERESEAPTARG"
     gene            1982..4024
                     /locus_tag="VLF19_03820"
     CDS             1982..4024
                     /locus_tag="VLF19_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HSE92410.1"
                     /translation="MRSVCPHDCPSACALLVTVRDGHLAEVTGDPAHPFTRGVICGKV
                     RAYAERVHSPLRVLYPLRRAGAKGDGRFERIAWDDAIGEIADRWRAIIARQGAEAILP
                     FSYAGTMGQVQYFAGHPLFHALGASQLDRTICVATAYAGWRATLGTVTGNDSEQMVGA
                     DLVVLWGVNAAYSTINVMTLVKEARRRGAYVVAIDPYRTPTVEQADEHLMVRPGTDTA
                     LALGVMHVLAAEGRVDHDYVARATLGYERLAEHVRAWPPERVAAITGVSAEAVVAFAR
                     RYGAGPRSFIRVGIGLSRHTNGGMACRTIACLPALTGAWADPHGGALLSSAGAYGFAA
                     RVFERADLMPAPVPRTINMIQLGRALTDPRLAPPVQSLYVYNSNPAAVCPDQRRVLEG
                     LARQDLFTVVHEQVMTDTAYHADLVLPATTSMEHEDLYHSFGHLHLQLALPVLAPPGE
                     ARSNWDVFGMLARALGVAGAHYAKTPEQLVREALAGGGESMRGITYERLRAEGSVRLN
                     LPAPYLPFANGAPTRSGKVEFYSDRLAARGLPPLPTHVPLAEGPDDTDLSARYPLQCI
                     VPPNRFFLNSSFSQSEVLRARQKSPTVLMAAEDADARGIHDGDGVRVFNDRGAVRFTA
                     QVTDATRPGVVVAEGIWWHRFHPGGRGVNVLTSDRETDMGGGPAFHSNLVQIERAG"
     gene            complement(4501..>4903)
                     /locus_tag="VLF19_03825"
     CDS             complement(4501..>4903)
                     /locus_tag="VLF19_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92411.1"
                     /translation="FDLQLTLGNIFGIPLILGAAAEFGSNIVLRFMEGQAHGGPLVAR
                     STVMAVLVNGLTTIVGFGSLMIAHHRGVFGLGLLLTLGMVTSLVAALVVLPVLLRMVE
                     QMRVARRARRLVRTAVGRATTELERAEQPRI"
BASE COUNT          729 a         1734 c         1767 g          673 t
ORIGIN      
        1 ctgaacgacg agctgggcgc ctggcctggc gtgaagatca cgccgatgtt cgggcgctgg
       61 ggctacttcg tgggccggca gctcttcgcg tgcttcccgc tgcgcgagaa ggaccgcgac
      121 ctgtgggtga ggctcgggac cgacgatcag gcgcgggcgc tccgcgacgc gcgcgtgcgg
      181 ccgcaccggc ggttcgcccg gcgcggatgg gtcgagctga acgtcgagga gccggccgac
      241 ctggtcctgg cgctccgctg gctgcggcgc gcgcacgcgg cggcggcccg cggcctcgag
      301 gaggactgag atgccggctc cgctacggcg aggggcaccg gaacacctcg gccaccatgg
      361 ccttgccgat ctcgatgccg aagggaccgc ggtcgggctt ctcgccgatg atcagcgcat
      421 gcgtgccgcc gacttctccg gccttcctgg cgaggtccac gtaatccgtg tccgacacga
      481 gacccacgta ctgacactgc tggacggcct gcacgtcctt gacgacacgg gccgggatag
      541 gtttggcgca ggcggtggcc aggaccaggg ccacgagtcc gatcgtgcag atgcgggcga
      601 cggtcatggc ggccctccat tccccgccgt ctgccgcggg ggcaccttcc gtcggggacg
      661 acgcggcgcc gccgcccgtc cgccgggcta gccccgggcc gtcggcgcct cggactcgcg
      721 ctccgcccac gccttggcct cgatgcgatc gatgcggacc agcggcacca gcggccacgc
      781 gagcacggtg agtgccgccg agatcaggac gagcggcgtg taaccgaagc catcgtacag
      841 gcgcgcgccg acgttctgcg agagctgcac gccggcgttg tacaccgaca tcagcagtgc
      901 gaagaaggtc gcctccacgc gccgcgggca ggccttggcc gccaggtcca ggaaggcgag
      961 ctgggtgatc atggccacga cgccgaagag gacctggatg ccgatcgccg agatgcggtc
     1021 acggtagaac agataggcga gggtgctgac cagcgcggca ccgatggcgg cgacgatcag
     1081 acgcttgagc ggcatccgtc gcgacagcgg ggcgtagacg atcgcgcccg ccatcgccgc
     1141 cccgtagtac atcgagtcga gcgcgccgat gaactcctgg ctgaacttca ggacgtccgt
     1201 ctggtagtac aggaacgcgg gtccgaacga cgggctgaac gtgtagaaga agatgaagcc
     1261 cgcgaccacc catacgctgc ggtcggtcat ggccgtccgg atcgcgcgcc acgtctcgcg
     1321 gacggcctcg cggtccacca cgccgggtcg ctcgtcgatg aaccgcaggc ccatgaccag
     1381 gctgacgacg ggcaagcacg ccgccagcgc gaaggccagc gcgaggttgc gggcgccggc
     1441 cagctgtccg ccgaggaggc cgacggcgac cgaggcggcc gtgatggccg cccactgcac
     1501 ggactggaag gcgccggtga ggccgcgggg ccggccgttc tccaccatga gcgcgtcggt
     1561 gagcacgtcg gtgaaggcca ggcctagccc catgatcgtg aacatcacca tcatcgcccg
     1621 gtacggatgc cccgcccacg acgccagtgc cgtgccggcc gcgatcgcca gcgacgtcgt
     1681 cagcaggtag tagctcttgc ggcgacggcc ccagagcggg acgaagtccg agatcaggcc
     1741 gtagacgggc ttgatcagcc aggggacggt cgccaccagg aagaagtcgg cgacttgccc
     1801 ggccgagagc ccgcgctcct tgaggacgat cgtgatcgtc tggttgggca ggtaccacat
     1861 gccctgggcg aagtagacgg tgccgaacag cagggcgagg cgctgggcgt ccggagtctt
     1921 gaacgggtgc agccggcgga gccgctcgag catgcgccgg ccatgatacc ttgacaacgc
     1981 catgcgctcg gtgtgtccgc acgactgtcc gtcggcctgt gcgctgctcg tcaccgtgcg
     2041 cgacggccac ctggccgagg tgaccggcga tccggcgcat ccgttcaccc ggggcgtcat
     2101 ctgcggcaag gtgcgcgcct acgccgagcg tgtccactcg ccgctgcgcg tgctgtaccc
     2161 gctccggcgg gccggtgcga agggcgacgg gcggttcgag cgcatcgcct gggacgacgc
     2221 gatcggcgag atcgccgacc ggtggcgcgc gatcatcgcc cgtcagggcg ccgaggcgat
     2281 tctgcccttc tcctatgcgg ggacgatggg gcaggtgcag tacttcgccg gccacccgct
     2341 cttccacgcg ctcggcgcga gccagctcga ccgcacgatc tgcgtcgcca ccgcctacgc
     2401 cggctggcgt gcgaccctcg gcaccgtgac cggcaacgac tccgagcaga tggtgggcgc
     2461 cgacctcgtg gtgctctggg gcgtcaacgc cgcctactcc acgatcaacg tgatgacgct
     2521 ggtcaaggag gcgcgccggc gcggcgcgta cgtcgtcgcc atcgacccgt accggacccc
     2581 gaccgtcgag caggcggacg agcacctcat ggtccgcccg ggcaccgaca cggcgctcgc
     2641 cctcggggtc atgcacgtcc tcgccgccga gggccgcgtc gaccacgact acgtcgcgcg
     2701 ggcgacgctc ggctacgagc gcctggccga gcacgtgagg gcgtggccgc cggagcgcgt
     2761 ggccgcgatc accggcgtgt cggcggaggc cgtcgtcgcc ttcgcgcggc gctacggcgc
     2821 gggcccgcgc agtttcatcc gggtcggcat cgggctctcg cgccacacca acggcggcat
     2881 ggcctgccgc acgatcgctt gcctgcccgc gctcaccggc gcgtgggccg acccgcacgg
     2941 cggcgcgctc ctgtcgtccg ccggcgccta cgggttcgcc gcccgcgtgt tcgagcgtgc
     3001 ggatctgatg ccggcgccgg tcccgcgcac gatcaacatg atccagctcg ggcgcgcgct
     3061 caccgatccg cgtctggcgc cgcccgtcca gtcgctctac gtctacaact cgaaccccgc
     3121 cgccgtctgt ccggaccagc gtcgggtgct cgaggggttg gcgcgccagg acctcttcac
     3181 cgtcgtccac gagcaggtca tgacggacac ggcgtaccac gcggacctcg tgttgccggc
     3241 gaccacgtcg atggagcacg aggacctcta ccattccttc gggcacctgc atctccagct
     3301 cgcgctgccg gtgctggcgc cgcccggcga ggccaggtcg aactgggacg tcttcggcat
     3361 gctggcgcgc gctctcggcg tggccggggc gcactacgcg aagaccccgg agcagctcgt
     3421 ccgggaggcg ctcgccggtg gcggcgagag tatgcgcggc atcacctacg agcggctgcg
     3481 cgcggagggc tcggtgcgtc tgaacctgcc ggcgccgtac ctgccgttcg cgaacggcgc
     3541 gcccacgcgc tcggggaagg tggagttcta ctccgatcgt ctggccgccc gcggcctccc
     3601 cccgcttccc acccacgtac cgctggcgga agggcccgac gacaccgatc tctccgcccg
     3661 ttacccgctg caatgcatcg tgccgcccaa ccggttcttt ctgaactcgt cgttcagcca
     3721 gtcggaggtg ctgcgcgccc ggcagaaatc gccgacggtg ctgatggcgg ccgaggacgc
     3781 cgacgcccgg gggatccacg acggcgacgg ggtgcgggtc ttcaacgacc gcggcgcggt
     3841 gcgcttcacg gcgcaggtca ccgacgccac gcggccgggc gtggtggtcg ccgagggcat
     3901 ctggtggcat cgcttccacc ccggcggccg cggcgtcaac gtgctcacct ccgaccgcga
     3961 gaccgacatg ggcggcggcc ccgcgttcca ctccaacctg gtccagatcg agcgggccgg
     4021 ctgatgcgcc gagtctggag gtcgtggacg aatacggccg gcgaacgtgc gactccgaga
     4081 ctagaatcag tgacgcccgc cgcgtaccgg aaggcacggg tgaagccgcg gcgacgcggc
     4141 cagcgctgca aaggcaggga ggctcgccgt caggtgagga acgtgtcctt gcgcggtcgc
     4201 cacggatagc cgccggagta acgcccggcc ggcgggcgcg attcgcgcca ggcccatccc
     4261 gtcagggcgc gcgtgtggca ccgatgccgc gtggttcgag cgccggcttc gccggcggaa
     4321 tcgatcctcc ggggtcgcga atcagaagtc gctggtgaac gcgccggccg ggacggggtc
     4381 ggcgaacctt cgactcggga gcctacacca cgagcctttc gggtcgacga ggcgcgccca
     4441 ggctccgccg gggcgtgacg aacgttgggg gcctgggggc catgtcgggt ctccatgtcg
     4501 tcagatgcga ggctgctcgg cgcgctcgag ctccgtggtc gcccgcccga ccgccgtgcg
     4561 gaccagccgg cgtgcgcggc gcgccacgcg catctgctcc accatgcgca gcagcaccgg
     4621 caacaccacc agcgcagcga cgagactggt caccatgccc agggtcaaca gcagccccag
     4681 gccgaacacg ccccgatggt gagcgatcat gaggctgccg aagccgacga tcgtggtgag
     4741 gccgttgacg agcacggcca tcacggtgct gcgcgcgacg agcgggccgc cgtgggcctg
     4801 gccttccatg aagcgcagca cgatgttgga gccgaactcg gcggcggcgc cgaggatgag
     4861 cggaatgccg aagatgttgc ccagagtgag ctgcaggtcg aag
//
LOCUS       DATIAZ010000112        18446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1606687, whole genome shotgun sequence.
ACCESSION   DATIAZ010000112 DATIAZ010000000
VERSION     DATIAZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 18446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18446
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606687"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1903
                     /locus_tag="VLF19_03830"
     CDS             47..1903
                     /locus_tag="VLF19_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSE92412.1"
                     /translation="MAPVTGAAKPSRGLERAVVRFAGDSGDGMQLTGEQFTTESAWAG
                     NDIATLPNFPAEIRAPAGTLFGVSSFQLQFGSQRVYTPGDRLDCLVAMNPAALKVHLR
                     DLKPGGLLIVNTSAFDKRNLDKAGYPTNPLDDPALAERYRLHKVDMTGLTLEAIKDLP
                     LNQKEKERTKNFFALGLVSWIYTRPLEPTLDWINKRFAKRAEIAEGNARVLKAGHAFG
                     ETAEIFGEHYTVEAADMAPGLYRAMTGNRALAWGLLAAAERSKVPIVYGAYPITPASS
                     ILEELAMHKRFRVRTIQAEDEIAAVTAAIGASFGGAIGVTGSSGPGIALKAEGIGLAV
                     TAELPLVIFNIQRAGPSTGMPTKTEQADLMQALYGRNSESPVVVVAPATPGDCFYVAY
                     EAVRIAAKYMVPVLVLSDGFLANGSEPWLIPDVSTLPPIEIRFRTEPEGFFAYLRDPA
                     TLARPWVCPGTPGLEHRIGGLEKQDVTGNISYDPENHDHMVRLRAEKVRRVAQEIPPT
                     SINGPATGDLLVVGWGGTYGAITAAVEHAQAEGKSVASVHLRHLNPLPPDLGHILREY
                     RRVLVPEINSGQLVRVLRAEYLVDAVGFNRVRGLPLASDEIHEAISQLLGST"
     gene            1904..2920
                     /locus_tag="VLF19_03835"
     CDS             1904..2920
                     /locus_tag="VLF19_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSE92413.1"
                     /translation="MGTGAADTHRYTKKDFESDQDVRWCPGCGDYAILSAIQKTMPDL
                     GIPKEDIVFISGIGCSSRFPYYMNTYGFHTIHGRAPAIATGLRLARPELKVFLVTGDG
                     DGLSIGGNHMLHVLRRNVNVTILLFNNRIYGLTKGQYSPTSELGKVTKSTPMGSADRP
                     VNPLSFALGCGATFVGRTVDRDVRHMEEMLKRAAHHTGAAFLEILQNCNVYNDLAWNA
                     LYDKESKLAHELRLEHGKPLLFGPPGDRKAVVMDGVIPKVIPATSGPERGLWVHDEQN
                     LNAARLLADLWAPEFPIPVGVLTAREAPVYEDVLLDQEQRAISERGPGDIAKLLTSGD
                     TWKI"
     gene            3074..4519
                     /locus_tag="VLF19_03840"
     CDS             3074..4519
                     /locus_tag="VLF19_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSE92414.1"
                     /translation="MLLSDAFVAELRGIVGAGGLVTTDEGRLVYECDMHTLYKGMPDV
                     VALPATTAEVEAIVRLCRRARVPVVPRGSGTGLIGGAMAPRGGVMVGLNRMNAILDVD
                     LPNRCATVQPGLINLWLTRAVQDRGWFFAPDPSSQMVSSIGGNVATNAGGPHCLKYGI
                     TTNHVLGLELVTGHGDVVRLGGKVADRPGYDLVGAAVGAEGTFGLVTAVTVRLLHAPE
                     AVKTVLASFATAVDASEAVSEIIARGIVPAALEMLDEVIIRAINGSLGKIYPDGAGAV
                     LLIELDGPRAEVEAQASDVVAICRRRGALSVNVARDEAERAVLWKGRKEAAGAVGRIT
                     SSYLLQDAVVPRSRLTQIMRELEAIAARHRVTIANVFHAGDGNLHPLICYDDRRPDEL
                     ARASQANEELLRACIALGGSVTGEHGVGLDKAKNLPLQYGEADLNFMYRLRRVFDPDA
                     IMNPGKLLPAHPACGEGFRPRKPDVPAGTWV"
     gene            4527..5840
                     /locus_tag="VLF19_03845"
     CDS             4527..5840
                     /locus_tag="VLF19_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSE92415.1"
                     /translation="MTERLIGAALVRIAGRPGVIDDAAALAAAAVDGRTPRWVVTPRS
                     VEQVAAVLALAREEGLAVLPRGSGSALDLGHPPAALDVVLETRGLARVIDYNPDDLTV
                     SVEAGVLAGTLGGRLSARRQWLPIDPAGVAARTVGGLTATGAAGPLRLRYGTLRDLLL
                     GVRFVQADGVVTWGGARVVKSVTGYDVPKLMVGALGTLGVLVEMTFRLHPRPPGETTW
                     LASFPAADGAQAFAAAVLDSPLQPNRLEALNGVALRACAVDPAPIAVAVTIGSVEAAV
                     REQGVLAEQLAARCGGAWTPAPSDFWARWDHAFAKGEDDVVLMVSSPASRLRETLGAI
                     EARHGALTAGADPMVSGCAALGALRVTLTGGDAGEVAALVEGLRGDLGPHGGGVVVQA
                     ARAGLRAQVDPWGPIDPGALGLMKALKDEFDPTRVLNPGRFVGGL"
     gene            5870..7099
                     /locus_tag="VLF19_03850"
     CDS             5870..7099
                     /locus_tag="VLF19_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSE92416.1"
                     /translation="MALDHDALRTCVHCGICLPQCPTYRLLGEEMDSPRGRVYLMRAA
                     AEGRITPTRIFARHIDLCLGCRACETACPSGVPFGSLLEATRAELRRRGLTTQSPALA
                     KVICKLFPHPDRLRLALRAARIYQRTGLRAAVRASRLLRIAPRLAAMEALLPDVPTAG
                     RLRETIAPRGPRVGRAALLLGCVQRHLYPHVHRDTIRLLTLAGWEVSIPRRQGCCGAL
                     DLHAGRLGAYRARARALASLFRDADVVVTNAAGCGSAMRDWAHWLPEEVAADVARRVR
                     DVTQVLVDADLPLGPLHLTVTYHDACHLVHGQKVRAEPRALLERIPGLRLVPLADSDL
                     CCGSAGVYNLVEPEMADRLLEMKLARIAETGAPTVVTGNPGCLLQIAKGARARGLDLD
                     VLHPVELLMRSVNGSAN"
     gene            7109..7432
                     /locus_tag="VLF19_03855"
     CDS             7109..7432
                     /locus_tag="VLF19_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92417.1"
                     /translation="MKIRARDRAEFRPDRMAKVGLARTERALLDLYCLAPGQEQAPHA
                     HADQDKIYVVLEGRARVVLDGRAETLEPGEAVVAPAGKSHGIANPGPAPLLALVLVTP
                     PPPHA"
     gene            7429..8244
                     /locus_tag="VLF19_03860"
     CDS             7429..8244
                     /locus_tag="VLF19_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSE92418.1"
                     /translation="MSAVRAALMVTCLGDLFYPEVGVRIVRLLRRLGVQVEFPRGQTC
                     CGLPLFNSGYHAEAAAVAARTVRLFRDVEHVVVPSGSCAWMVKHEYPGLMTDPAHRRE
                     AEALAARTWELSQFLVSVMGRTHFASAVPGRVTYHDSCHLLRGLHESVTPRTILRSLG
                     GVEFVELGGADECCGFGGSFAVRLPEVSTAILDRKLASVEASGADCVVTCDAGCLMQM
                     GGGLRRRGSRVRALHLVEIISEGATTLSEVASTGPVGASPRAGWPGEADARKP"
     gene            8241..9644
                     /locus_tag="VLF19_03865"
     CDS             8241..9644
                     /locus_tag="VLF19_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HSE92419.1"
                     /translation="MTFRERTSGALGDPFLQEALTIATTKFIGLRRDAFGDFPEGEAL
                     RDQARAIKEATLQRLDHWLELLVDHVERLGGRVHYATTAEEARDVVLDIARRTGARMV
                     VKSKSMATEEIDLNEALERAGITPVETDLGEYIIQLAHEKPSHIIAPAIHKTKGQVAD
                     LFARELKRQVAADPEVLTRIAREELREKFLRADIGITGANFAVAETGTVVLVTNEGNG
                     RMATSLPRVHVAIMGVEKVVPSMTDLVVFLAILAKSATGQKLSVYTTLVQGPRRPGEL
                     EGPDEFHLVLIDNGRTGQIAGPLREALSCLRCGACLNVCPVYRRVGGHAYGYTYPGPI
                     GILLTAMLHGQRSVTELAHASSLCGACAEACPVRIDIPRMLVELRRGGVESRNAPWAE
                     RVAFRALARVLASPGLYRLAARLARGLQRPFARDGMIRRLPFVAGEWTRTRDLPAVAP
                     RTFAERWADLEREPPRR"
     gene            9641..10372
                     /locus_tag="VLF19_03870"
     CDS             9641..10372
                     /locus_tag="VLF19_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HSE92420.1"
                     /translation="MTTRAEFFARIRKEVGTTSGLFSAVPAARPAPPRRLLETLRREL
                     SERWPETLDRFRHEFECVGGVFYRVKSAAEVPDVVARIARERDARRVVTWHPSALGTD
                     FCAELERQGLEAEAMPSGGAPVSDDARAAVRARVAAADLGLTGVDLAIAETGTLVLVS
                     GAGRPRSTALLPSYHVAVFDREALVESLQQVGLVLEAWHDGTPPASRGAAIHFITGPS
                     RTADIELTLTRGVHGPREVHAVFVG"
     gene            10376..10777
                     /locus_tag="VLF19_03875"
     CDS             10376..10777
                     /locus_tag="VLF19_03875"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HSE92421.1"
                     /translation="MADRYFSPAEVEAMIPALTGVMTRLMAAYREAGEARDRLQTEQR
                     RIALSGGGILDGDAWRAGREDLGRSGAVIQEGLREIATMGGVPKDLGMGLVDFPHLRE
                     GREVNLCWRHGEWQIRFWHGLDEGYAGRKPL"
     gene            10783..11529
                     /locus_tag="VLF19_03880"
     CDS             10783..11529
                     /locus_tag="VLF19_03880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSE92422.1"
                     /translation="MAYRAVLFDLFDTLVRFDRERLPEVRVDGRVVRSTAGHLHPILA
                     SVAPQVTLDALYGALIESWKEAERQRAIDHREVAAPERFAHLFRCLALDPGVWSTDVL
                     QRLLDTHRRELSRAAEFPPHHRALLEALAGRYRLGVVSNFDYTPTAIGILETAGVAHL
                     FDTIVVSDTFGWRKPARSIFDEALRRVGTPPGEALFIGDRADIDVAGAQAAGMHAVWI
                     NPDREALPPGLAAPEYEIRDLDELRWILAV"
     gene            11567..12505
                     /gene="trxB"
                     /locus_tag="VLF19_03885"
     CDS             11567..12505
                     /gene="trxB"
                     /locus_tag="VLF19_03885"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017620592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSE92423.1"
                     /translation="MATTRKVIIIGSGPAGYTAAIYAARANLAPLMFTGVQSGGQLML
                     TTLVENYPGFVDGLMGPEMMENFRKQAERFGTEMVSDDVTAVDFSRRPFVVKVGDEAW
                     ESHAVILATGASAKLIGLENETKLMGRGVSTCATCDGFFFKDQDIMVVGGGDSAMEEA
                     LYLSRLGKTVTVVHRRDALRASKIMQERAFKNPKIEFVWDTAVDDVLDPAQGKVTAVK
                     LRNVKTGGLTERRVDGLFVAIGHRPNTEIFRGQIELMPNDYVKVRPGTTQTSMPGVFA
                     AGDVQDFTYRQAVTAAGSGCMAALDAERYLEATQAH"
     gene            complement(12732..13541)
                     /locus_tag="VLF19_03890"
     CDS             complement(12732..13541)
                     /locus_tag="VLF19_03890"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HSE92424.1"
                     /translation="MDTQRSVGVLGLGVIGRAVCRALDAGLPGLRLAGALARDRLKAE
                     AFLRTLDVEPPFLPLGDLIAASDILVEASTRAHLEEVAPVALAAGRDLVVLSCGALLD
                     HPEWVALAEAKRARIHVVSGAIAGLDGLKGARTGAMTSVVMETRKPPRGLAGAPWILE
                     NKIDLDAIREETLVFEGPARDACRAFPANVNVLAAVSLAGIGPERTRTRIYAVPGLAM
                     NRHRVRAEGEFGTFTIEIENVPSENPRTGKLSYLSTVALLRELRACLRVGT"
     gene            13600..14469
                     /locus_tag="VLF19_03895"
     CDS             13600..14469
                     /locus_tag="VLF19_03895"
                     /inference="COORDINATES: protein motif:HMM:NF021210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HSE92425.1"
                     /translation="MREPLERLYREFDYAARVERDAIRFPLRYDDPRDREIVALLTAC
                     LAYGRVDLFGRELDRVLTAMGPSPAAFVAGFDPCHHAATFAAFRYRFNRPRDLTAFCV
                     AARDLLARHGTLEKLFLAGDPDACGPVGPALERFARAFLDADLSDVFPRRRLSRGYRH
                     LFPVPSAGGPCKRMHLFLRWMVRREPPDFGLWASVSPARLLIPVDTHIENMSRAIGLT
                     RRRSRNWRMAEEITARLALVDPADPVKYDFALCHKRMSGGCRDRRDPVICTPCGLRSV
                     CRHWAKPLRQPRS"
     gene            14466..15854
                     /gene="rmuC"
                     /locus_tag="VLF19_03900"
     CDS             14466..15854
                     /gene="rmuC"
                     /locus_tag="VLF19_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSE92426.1"
                     /translation="MSETVIALVGLVVGGAAAWVLARAHFRSVALGEAQARESRLAVA
                     ESLADELRKQLSHRDLEAGDLRSTLQAERALRAQAEARAESTRENLEEQKRLLEGARE
                     RLTETFKALSADALRQSTSAFLEQARETIDGQLARRQEALDGVVKPLQESLKRYEAEL
                     RQLETSRQQAYGSLEEQLKTLNARSAELQRETGSLVTALRSPQVRGRWGELTLRRAAE
                     LAGMTPYCDYLEQVTVEGEGGRLRPDMIVRLPSGRTIAVDAKVPLNAYLDATGAVTED
                     ERGRLLGRHAQQVRQHLAVLSARAYWDQLDDSVELVVMFIPGEAFVGAAMQADARLLE
                     DGMAKKILIATPTTLIGLLLAIAHGWQQERLAVNAAQISELGRLLYERLRTLAGHVED
                     VGDKLGKAVHAYNAAVGSMESRVLPAARRFRDLGAAGGEEIPVLEAVDERPRSLTVPD
                     VPEQLRAPDLPA"
     gene            complement(15885..16709)
                     /locus_tag="VLF19_03905"
     CDS             complement(15885..16709)
                     /locus_tag="VLF19_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSE92427.1"
                     /translation="MSRAIGRPWLTVAGALVGGAVLSPYLVMFFTALKPDRELRTSPP
                     RLTPLDWQPGNFLEVLRDPAFQSWLQVSLVVALASTALVILAAVPAAYYTARHRFPGR
                     TAFLFVVLVTQMFAPTALVVGLYREFFELDLVNTYAALILTNAAFNLAFAIWILRAFF
                     ASIPREIEEAAAVDGCGRVRCLARIVLPLSLPGIVTAVIFAFIGAWNEYIVALTLMTD
                     PDMKPLTVGITTYVTAYVQHWNLLFAASVIAIAPVVVLFALIERYLVGGLTAGSLK"
     gene            complement(16723..17535)
                     /locus_tag="VLF19_03910"
     CDS             complement(16723..17535)
                     /locus_tag="VLF19_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSE92428.1"
                     /translation="MYPAVQLGAASLSRYSVTGLYLGSVGTRNYAALFRQDALPAVLA
                     NTMIWVTVVVAVTIVISLGLAQLLDQSFPGRRLVRWALIVPWAASLIMTSKLFVWIYD
                     YYFGILNHVLLSLSLIGHPLDWLGDDATVMGAMIAVGVFVSLPFTTYVFLAGLQTIPG
                     DVYEAARVDGASLWRTYRSVTLPLLRPALLVAAVLNIISVFNSFPIVWTLNDRNPGFG
                     HDTMVTYMYKIAFKSALRDVGLAAALGVVNVLVILLAVVVYVKMVRWREQEA"
     gene            complement(17732..>18446)
                     /locus_tag="VLF19_03915"
     CDS             complement(17732..>18446)
                     /locus_tag="VLF19_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE92429.1"
                     /translation="ALGEGTIGYALPLGPEEAQAEWSIWMWNNGGDWKAGGVWAINSD
                     RNVQTLQFLADLAHRHKVTQVNPGKTNRTDGAFQLFKDGKVGMVMGFSPLAAQLDAEG
                     KVDYGVAEMPTNVGTAVTLGVEDYLMAFRKKRNQEAVRQFLDLYYEPRNVTRWITAEG
                     FLPVTRSGLTQMSGNPRLAPYLAALPNARLVPTTDPAWDKVKLDVQQTIGLAVQPGGN
                     PKQVLDQLQQRATAAASAR"
BASE COUNT         2655 a         6517 c         6629 g         2645 t
ORIGIN      
        1 gccgatcact cgagccaaga gagggcgact gtcgatgagc gagacgatgg ctcccgtcac
       61 gggcgccgcc aaacccagca gggggctcga gcgtgccgtc gtccgcttcg cgggcgactc
      121 cggtgacggc atgcagctga cgggggagca gttcacgacc gagtcggcct gggccggcaa
      181 cgacatcgcg acgctgccga acttccccgc cgagattcgc gcccccgccg gtacgctctt
      241 cggcgtctcc agcttccagc ttcagttcgg cagccagcgc gtctacaccc cgggtgaccg
      301 gctcgactgc ctggtggcga tgaatccggc agcgctcaag gtgcacctgc gcgacctcaa
      361 gccgggcggg ctcctgatcg tcaacacctc ggcgttcgac aagcggaacc tcgacaaggc
      421 cgggtacccg acgaatccgc tggacgatcc ggctctcgcg gagcgctacc gcctgcacaa
      481 ggtcgacatg acggggctca cgctcgaggc gatcaaggac ctgccgctga accagaagga
      541 gaaggagcgc acgaagaact tcttcgcgct cggcctcgtc tcctggatct acacgcggcc
      601 cctggaaccg acactcgact ggatcaacaa gcgcttcgcc aagcgcgcgg agatcgccga
      661 gggcaacgcc cgcgtcctga aggccggtca tgccttcggc gagaccgcgg agatcttcgg
      721 cgagcactac acggtcgagg ccgccgacat ggcgccgggc ctctaccgcg cgatgaccgg
      781 caatcgcgcg ctcgcgtggg gcctgctggc ggcggccgag cgctccaagg tgccgatcgt
      841 ctacggcgcg tacccgatca cgccggcgag cagcatcctg gaagagctgg cgatgcacaa
      901 gcgcttccgt gtccgcacga tccaggccga ggacgagatc gccgcggtca ccgcggccat
      961 cggcgcgtcc ttcggcggcg ccatcggcgt gacgggctcg agcgggcccg gcatcgcgct
     1021 caaggccgag gggatcggtc tcgccgtcac cgcggagctg ccgctcgtca tcttcaacat
     1081 ccagcgggcg gggccctcga cgggcatgcc caccaagacc gagcaagccg acctcatgca
     1141 ggcgctctat gggcgcaaca gcgagtcgcc tgtggtggtg gtggctccgg cgacgcccgg
     1201 cgactgcttc tacgtcgcct acgaggccgt gcgcatcgct gcgaagtaca tggtgcccgt
     1261 cctcgtgctc tccgacgggt tccttgccaa cggctccgag ccgtggctga tccccgacgt
     1321 cagcacgctg ccgcccatcg agatccggtt ccggaccgag ccggagggct tcttcgcgta
     1381 cctgcgcgac cccgccacgc tggcgcggcc gtgggtgtgc ccgggcacgc cggggctcga
     1441 gcaccggatc ggcggcctcg agaagcagga cgtgacgggc aacatctcct acgaccccga
     1501 gaaccacgac cacatggtgc gactccgtgc cgagaaggtg cggcgcgtgg cccaggagat
     1561 tccgccgacg tccatcaacg gcccggccac gggcgacctc ctcgtcgtcg gctggggcgg
     1621 tacctatggc gccatcaccg ccgcggtcga gcacgcgcaa gcggagggca agtccgtggc
     1681 ctcggtccat ctccgccacc tcaacccgct gccccccgac ctcggccaca tcctgcgcga
     1741 gtaccgcagg gtgttggtgc cggagatcaa cagcgggcag ctcgtgcggg tgctgcgcgc
     1801 cgaatacctc gtggacgccg tgggcttcaa ccgcgtccgc ggtctgccgc tggcgtccga
     1861 cgagatccac gaagccatca gccagttgct ggggagcacg tagatgggta cgggggcggc
     1921 ggacacgcat cggtacacca agaaggactt cgagtcggac caggacgtgc ggtggtgccc
     1981 aggctgtggg gactacgcga tcctgtcggc catccagaag acgatgccgg acctcggcat
     2041 cccgaaggag gacatcgtct tcatctccgg gatcgggtgc tcgagccgat tcccgtacta
     2101 catgaacacg tacgggttcc acacgatcca cggacgcgcg ccggcgatcg ccaccggcct
     2161 gcgtctggcg cggcccgagc tgaaggtctt cctggtcacc ggcgacggtg acgggctctc
     2221 gatcggcggc aaccacatgc tgcacgtgct ccgccgcaac gtgaacgtca cgatcctgct
     2281 cttcaacaac cggatctacg gactcaccaa gggccagtac tcgccgacca gcgagctggg
     2341 caaggtgacg aagtccacgc cgatggggtc ggccgaccgc ccggtgaacc cgctgtcgtt
     2401 cgcgctcggc tgcggcgcca ccttcgtggg ccgcacggtg gacagggacg tccggcacat
     2461 ggaagagatg ctcaagcgcg cggcgcacca caccggcgcg gcgttcctcg agatcctcca
     2521 gaactgcaac gtgtacaacg acctcgcgtg gaacgcgctg tacgacaagg agtcgaagct
     2581 ggcgcacgag ctccgactcg agcacggcaa gccgctgctc ttcgggcctc ccggcgaccg
     2641 caaggccgtg gtcatggacg gcgtgatacc gaaggtcatc cccgccacga gcgggccgga
     2701 gagggggctc tgggtgcacg acgagcagaa cctcaacgcg gcccggttgc tcgccgacct
     2761 ctgggcgccg gagttcccga ttcccgtcgg cgtgctgacc gctcgcgagg cgccggtgta
     2821 cgaggacgtg ctcctcgacc aggagcagcg ggccatctcg gagcgcggtc cgggcgacat
     2881 cgccaagctc ctcacgtccg gcgacacctg gaagatctag ctcggagggg ggcgtcgtcc
     2941 cccttccgaa gatcatcacg cgccttgcgg cgtgcgtctg cctgcctctc ccgggagggt
     3001 ctgcgccagc agagccggcg cgggaagcgt ggatcaatcc tgcctgcgat acactctcgt
     3061 gcggagactt tccatgctgc tgagtgacgc gttcgttgcg gagcttcgcg ggatcgtcgg
     3121 cgcggggggg ctcgtcacga cggacgaggg gaggctggtg tacgagtgcg acatgcacac
     3181 cctctacaag gggatgcccg acgtcgtggc gctgccggcc acgacggcgg aggtggaggc
     3241 gatcgtgcgg ctctgccggc gggcgcgcgt gccggtggtg ccacggggat cggggacggg
     3301 gctcatcggc ggcgccatgg cgccccgggg cggcgtcatg gtcggcctca atcgcatgaa
     3361 cgcgatcctc gatgtcgacc tgccgaaccg ttgcgccacc gttcagcccg gcctgatcaa
     3421 cctctggctc acccgcgcgg tgcaagatcg gggctggttc ttcgcgccgg acccatcgag
     3481 ccagatggtc tcgtccatcg gcggcaatgt cgccaccaat gcgggcggcc cgcattgcct
     3541 caagtacggc atcacgacga accacgtgct gggtctcgag ctggtcaccg gccacggcga
     3601 tgtcgtgcgc ctgggtggta aggtggcgga ccgtcccggc tacgatctcg tcggtgccgc
     3661 ggtgggcgcc gagggcacgt tcgggctcgt caccgcggtc acggtcaggc tcctgcacgc
     3721 gcccgaggcg gtgaagaccg tgctcgcctc gttcgcgacg gcggtggacg cctcggaggc
     3781 cgtgtcggag atcatcgcca ggggcatcgt ccccgccgcg ctggagatgc tggacgaggt
     3841 catcatccgg gcgatcaacg gcagcctcgg caagatctat cccgacggcg ccggcgccgt
     3901 gctgctgatc gagctcgacg gccctcgcgc cgaggtcgag gcgcaggcca gcgacgtggt
     3961 ggcgatctgc cgccggcgcg gcgcgctgag cgtgaacgtg gcccgtgacg aggccgagcg
     4021 cgcggtgctg tggaagggcc gcaaggaagc ggcgggcgcc gtcggccgga tcacctcgag
     4081 ctatctcctc caggacgccg tggtgccccg ctcgcgcctg acgcagatca tgcgcgagct
     4141 ggaggccatc gctgcgcggc atcgcgtgac catcgccaac gtgttccacg ccggggacgg
     4201 caacctccat ccgctgatct gctacgacga ccggaggccc gacgagctcg cgcgcgcctc
     4261 ccaggccaac gaggagctgc tgcgcgcgtg catcgcgctc gggggcagcg tgaccggtga
     4321 gcatggtgtc ggactcgaca aggccaagaa cctcccgctc cagtacggcg aggcggatct
     4381 caatttcatg taccgcctgc gccgggtctt cgaccccgac gcgatcatga atcccggcaa
     4441 gctcctgccg gcccatccgg cgtgcgggga ggggttccgc ccgaggaagc ccgacgtgcc
     4501 cgcggggacg tgggtctgac gtgtcgatga cggagcgctt gatcggtgcg gcactggtcc
     4561 ggatcgccgg ccggcccggc gtgatcgacg acgccgcggc cctcgccgcc gccgccgtcg
     4621 acgggcgaac gccgcggtgg gtggtcacgc cacggtccgt ggaacaggtc gccgctgtgc
     4681 tggcgctcgc ccgcgaggag ggcctggccg tcctgccacg cggcagcggc agtgccctcg
     4741 atctcggcca tccgcccgcg gccctcgacg tcgtcctgga gacgcgcggt ctcgcgcgcg
     4801 taatcgacta caaccccgat gacctcacgg tgagcgttga ggctggcgtg ctcgccggga
     4861 cgctgggcgg gcgcctcagc gcgcggcgac agtggctgcc gatcgaccct gccggcgtgg
     4921 ccgcacgcac cgttggtggc ctcaccgcga ccggcgccgc cggtcccctg cgactccgct
     4981 acggcacgct gcgggatctg ctcctggggg tgcgcttcgt ccaggccgac ggcgtggtca
     5041 cgtggggggg cgcgcgcgtc gtgaagtcgg tcaccgggta cgacgtcccg aagctcatgg
     5101 tcggcgccct cggcacgctc ggggtgctcg tcgagatgac gttccgactg catccccgtc
     5161 cgccaggcga gacgacgtgg ctcgcctcgt tccccgcggc cgacggcgcc caggccttcg
     5221 ccgccgccgt cctcgactcg ccgttgcagc cgaaccggct cgaggcgctc aacggcgtcg
     5281 ccctgcgcgc gtgcgccgtg gatcccgcgc cgatcgccgt cgccgtcacc atcggcagcg
     5341 tcgaggccgc cgtgcgcgag cagggcgtgc tcgccgagca actcgccgcg cgctgcggcg
     5401 gcgcgtggac gccggcgcca tcggacttct gggcgcgctg ggaccacgcc ttcgccaaag
     5461 gggaggacga cgtcgtcttg atggtgtcgt cgccggcgag ccggctccgc gagaccctgg
     5521 gggcgatcga ggcgcggcac ggcgcgctca cggccggcgc cgacccgatg gtgagcgggt
     5581 gtgccgcgct cggcgcgctg cgcgtgacgc tcaccggcgg cgatgccggt gaggtggcgg
     5641 cgctggtgga ggggctccgc ggggacctgg ggccgcacgg cggcggggtc gtcgtccagg
     5701 ccgcgcgggc ggggctgcgg gcacaggtcg atccgtgggg gcccatcgac ccgggcgcgc
     5761 tcggcctcat gaaggcgctc aaggacgagt tcgaccccac gcgtgtcctc aaccccggcc
     5821 gcttcgtggg cgggctctga tgtccgggcc gcccgcgccc gcggtcccga tggcgctgga
     5881 ccacgatgcg ctcaggacct gcgtgcactg tggcatctgc ctgccgcaat gtccgacgta
     5941 ccggctgctc ggcgaggaga tggattcgcc gcgcgggcgc gtctacctca tgcgcgcggc
     6001 cgccgagggg cgcatcacgc cgacgcggat cttcgcgcgc cacatcgacc tgtgtctcgg
     6061 ctgccgtgcc tgcgagaccg cgtgtccgtc cggcgtgccg ttcggctcgc tcctggaggc
     6121 cacgcgcgcc gagctccgcc ggcgcggcct caccacccag tcgcctgcgc tcgcgaaggt
     6181 gatctgcaaa ctcttcccgc accccgaccg gctgcggctg gcgctccgtg ccgcgcggat
     6241 ctaccagcgc accgggctgc gcgcggcggt gagagcgagc cgactgctga ggatcgcgcc
     6301 gcgcctcgcc gcgatggagg cactgctgcc cgacgtgccg accgccgggc gactgcggga
     6361 gaccatcgcg ccccgcggcc cgcgtgtcgg ccgcgccgcg ctcctgctcg gctgcgttca
     6421 gcgtcacctc tatccgcacg tccaccgcga caccatccgc ctgttgaccc tcgctggctg
     6481 ggaggtgtcg ataccgcgcc gccaggggtg ctgcggcgcg ctggacctcc acgcgggccg
     6541 cctcggggcg taccgcgccc gggcccgcgc gctcgcgtcg ctctttcgtg acgccgatgt
     6601 cgtcgtcacc aacgccgcag ggtgcgggtc ggcgatgcgg gactgggccc actggctccc
     6661 ggaagaggtg gccgcggacg tcgcccggcg agtgcgggat gtgacgcagg tgctggtgga
     6721 cgccgacctg ccgctcggtc ccctccatct gacggtgacc taccacgacg cctgtcacct
     6781 cgtgcacggc cagaaggtcc gtgccgagcc gcgcgcgctg ctcgagcgga tcccgggcct
     6841 gcgcctggtc ccgctggccg acagcgacct gtgctgtggc agcgccggcg tctacaatct
     6901 ggtggagccc gagatggcag atcgcctgct cgagatgaag ctcgcccgca tcgcggagac
     6961 gggggctccc accgtggtga ccggcaaccc cggttgcctg ctgcagatcg ccaagggcgc
     7021 ccgggcgcgc ggcctggacc tcgatgtgct ccatccagtg gaactgctca tgcggtcggt
     7081 gaacggcagt gcgaactgag ctcggccagt gaagatccgc gccagggacc gcgccgagtt
     7141 ccgccccgac aggatggcca aggtcggtct cgcccggacc gagcgcgcgc tcctcgacct
     7201 ttactgtctg gccccgggcc aggagcaggc cccgcacgcc cacgccgacc aggacaagat
     7261 ctacgtcgtc ctcgagggcc gcgctcgggt ggtcctcgac gggcgggccg agaccctcga
     7321 acccggcgag gccgtggtcg cgccggccgg caagtcccac gggatcgcga acccggggcc
     7381 cgcgccgctg ctggcgctcg tcctcgtcac gccaccgccg ccccacgcat gagcgccgtg
     7441 cgcgccgccc tgatggtcac gtgtctcggt gacctgttct atcccgaggt cggcgtgcgc
     7501 atcgtgcggc tcctgcgacg tctgggcgtg caggtcgagt tcccgcgcgg acagacgtgc
     7561 tgcgggctgc cgctcttcaa ttccggatac cacgccgagg cggctgccgt cgccgcgcgc
     7621 acggtgcggc tgttccgcga cgtcgagcac gtggtggtac cgtcgggctc gtgcgcgtgg
     7681 atggtcaagc acgagtaccc gggtttgatg acagatcccg cgcaccgccg cgaggccgag
     7741 gcgctggcgg cgaggacgtg ggagctgtcc cagttcctcg tctcggtcat gggccgcacg
     7801 cacttcgcga gcgccgtgcc cgggcgggtg acctatcacg actcctgcca tctgctgcgc
     7861 gggctccacg agtcggtcac gccgcgcacg atcctccgca gcctcggcgg cgtggagttc
     7921 gtggagctcg gcggcgccga cgagtgctgc ggcttcggcg gctcgttcgc cgtgcggctg
     7981 ccggaagtct cgacggccat cctcgaccgg aagctcgcca gcgtggaggc cagcggcgcc
     8041 gactgcgtgg tgacgtgcga cgccggttgc ctcatgcaga tggggggcgg gctgcgccgc
     8101 cgcgggtcgc gggtccgggc gctgcacctg gtcgagatca tctcggaagg ggccacgacc
     8161 ttgtcggagg ttgcctcgac gggccccgtc ggggcttccc ccagggcggg ttggcccggc
     8221 gaagccgacg cgcggaagcc atgacgttcc gcgagcggac atccggcgct ctgggggacc
     8281 ccttcctgca ggaggcgctg acgatcgcga cgacgaagtt catcgggctg cggcgggacg
     8341 ccttcggcga cttccccgag ggagaggcgc tccgcgacca ggcgcgcgca atcaaggaag
     8401 cgacgctgca gcggctcgac cactggctgg agctcctggt cgaccacgtc gagcggctgg
     8461 gcggccgcgt gcactacgcg acgacggccg aggaggcgcg cgacgtggtg ctcgacatcg
     8521 cgcggcggac cggcgcccgg atggtcgtga agagcaagtc gatggccacc gaggagatcg
     8581 acctgaacga ggcgctggag cgcgcgggca tcacgccggt cgagacggac ctcggcgagt
     8641 acatcatcca gctcgcccac gagaagccgt cgcacatcat cgccccggcg atccacaaga
     8701 cgaaggggca ggtggccgat ctcttcgccc gcgagctcaa gcggcaggtc gccgcggacc
     8761 ccgaagtcct cacgcggatc gcgcgcgagg agctgcgcga gaagttcctg agggccgaca
     8821 tcggcatcac cggcgccaac ttcgcggtgg ccgagaccgg cacggtcgtg ctcgtgacga
     8881 acgagggcaa cgggcgcatg gcgacctcgc tgccccgcgt gcacgtggcg atcatgggcg
     8941 tcgagaaggt cgtgccctcg atgacggacc tcgtggtgtt ccttgccatc ctcgcgaaga
     9001 gcgccaccgg gcagaagctg tccgtctaca cgacactcgt gcagggcccg cggcgaccgg
     9061 gcgaactcga gggaccggac gagttccacc tggtcctgat cgacaacggc cggaccggcc
     9121 agatcgccgg gccgctgcgc gaggcgctct cctgcctgcg gtgtggcgcc tgcctgaacg
     9181 tgtgcccggt ctatcgtcgg gtcggcggcc acgcctacgg ctacacgtac ccgggaccca
     9241 tcggcatcct cctcacggcg atgctccacg gccagcggtc ggtcaccgag ctggcccacg
     9301 cctcgtcgct ctgcggcgcg tgcgcggagg cctgtccggt ccgcatcgac atcccgcgga
     9361 tgctcgtcga gttgcgccgg ggcggggtgg agtcgaggaa tgcgccctgg gcggagcggg
     9421 tcgcctttcg cgccctcgcg cgcgtgctcg cgtcgccggg cctgtatcgg ctcgccgccc
     9481 ggctcgcgcg cgggctccag cgtccgttcg cccgcgacgg gatgatccga cgcctgccct
     9541 tcgtggccgg cgagtggacg cgcacgcggg atctcccggc cgtggcccca cggaccttcg
     9601 ccgagcgctg ggcggacctg gagcgggagc caccccggcg atgaccacgc gcgcagaatt
     9661 cttcgcgcgt atccgcaagg aggtcgggac gacgtcgggg ctcttctctg cggtcccggc
     9721 cgcgcggccg gcgccgccgc gccggctgct cgagacgctg cgccgcgagc tctcggagcg
     9781 ctggccggag acgctcgatc gcttccgcca cgagttcgag tgcgtcggcg gcgtctttta
     9841 ccgtgtcaag tccgcggccg aggttccgga cgtcgtggct cgcattgcgc gggagcgcga
     9901 cgcccggcgc gtcgtgacgt ggcatccatc ggccctcggc accgacttct gcgccgagct
     9961 ggagcgccag gggctcgagg ccgaggcgat gccgtcgggc ggcgcgccgg tctccgacga
    10021 tgcgcgagct gccgtgcgcg cgcgcgtcgc cgcggccgac ctgggcttga ccggtgtgga
    10081 cctggcgatc gccgagaccg gtaccctcgt gctggtctcc ggcgccggcc ggccgcgctc
    10141 gacggcgctg ctgccctcgt accacgtcgc ggtcttcgac cgcgaggcgc tcgtggagtc
    10201 gctccagcag gtgggcctcg tgctcgaggc gtggcacgac ggcaccccgc cggcctcgcg
    10261 cggcgccgcg atccacttca tcacggggcc gagccgcacc gccgacatcg agctcaccct
    10321 cacccgcggc gttcacggac ccagggaagt gcacgccgtc ttcgtgggct aggtcgtggc
    10381 cgaccggtat ttctcccccg cagaggtcga ggcgatgatc ccggcgctga cgggggtcat
    10441 gacgcggctc atggcggcgt accgcgaggc cggcgaggcg cgtgaccgct tgcagactga
    10501 gcagcgccgg atcgcgctct ccggcggcgg catcctcgac ggcgatgcct ggcgcgccgg
    10561 ccgggaagac ctggggcggt cgggtgcggt gatccaggag gggctgcgcg agatcgcgac
    10621 catgggaggc gtgcccaagg acctcgggat gggcctcgtc gactttccgc acctgcgcga
    10681 gggccgcgag gtgaacctct gctggcgcca tggggaatgg cagatccggt tctggcacgg
    10741 tttggacgaa ggctacgccg gccgcaagcc gctctgaatc cgatggccta ccgcgcggtc
    10801 ctcttcgacc tcttcgacac gctggtgcgg ttcgaccgcg agcgcctgcc cgaggtccgg
    10861 gtcgacggtc gcgtcgtgcg gtcgaccgcg ggacatctgc acccgatcct cgccagcgtg
    10921 gcgccccagg tgacgctgga cgcgctctac ggcgccctga tcgagagctg gaaggaggcc
    10981 gagcggcagc gcgccatcga ccaccgggag gtggccgcgc ccgagcgctt cgcccacctc
    11041 ttccggtgcc tggcgctcga tcctggcgtg tggtcgaccg acgtcctgca gcgactgctc
    11101 gacacgcacc ggcgcgagct ttcgcgggcc gccgagttcc cgccgcacca tcgggcgttg
    11161 ctcgaggcgc tggcaggtcg ctaccgtctg ggcgtcgtgt cgaacttcga ctacacgccc
    11221 acggcgatag ggatcctcga aaccgccggc gtggcgcacc tcttcgacac gatcgtcgtc
    11281 tccgacacct tcggctggcg caagccggcg cgctcgatct tcgacgaggc gctgaggcgg
    11341 gtcggcacgc cgcccggcga ggcgctgttc atcggggacc gggcggacat cgacgtggct
    11401 ggggcccagg cggccggcat gcacgccgtc tggatcaatc ccgaccgcga ggccctgcca
    11461 cccggcctgg cggcacccga gtacgagatc cgcgacctcg acgagctgcg gtggatcctc
    11521 gcggtctgac cgcgcatcgt aagaatggca aggagaacgg agcggcatgg cgacgacgcg
    11581 caaggtgatc atcatcgggt ctggccccgc cggttacacg gcggccatct acgcggcgcg
    11641 ggccaacctc gctccgctca tgttcaccgg cgtgcagagc ggaggccagc tcatgctgac
    11701 cacgctggtc gagaactacc cgggcttcgt cgacggcctc atgggcccgg agatgatgga
    11761 gaacttccgc aagcaggccg agcggttcgg caccgagatg gtctccgacg acgtcaccgc
    11821 cgtggacttt tcgcgccgcc ccttcgtcgt gaaggtcggg gacgaggcgt gggagagcca
    11881 cgcggtcatc ctcgccaccg gcgcgtcggc gaagctcatc ggcctcgaga acgagacgaa
    11941 gctcatgggc cgcggcgtgt cgacgtgcgc gacctgtgac ggcttcttct tcaaggacca
    12001 ggacatcatg gtcgtcgggg gtggcgactc cgcgatggag gaggccctct acctctcgcg
    12061 cctcggcaag acggtgacgg tggtccaccg gcgcgacgcg ctcagggcgt caaagatcat
    12121 gcaggagcgc gccttcaaga accccaagat cgagttcgtg tgggacacgg cggtcgacga
    12181 cgtgctcgac ccggcccagg gcaaggtcac ggccgtcaag ctgcgcaacg tgaagaccgg
    12241 cgggctcacg gagcggcgcg tcgacgggct gttcgtcgcc atcgggcacc ggcccaacac
    12301 ggagatcttc cgcggccaga tcgagctgat gcccaacgac tacgtgaagg tgaggccggg
    12361 caccacgcag acgtcgatgc cgggcgtctt cgccgccggc gacgtgcagg acttcaccta
    12421 ccgacaggcg gtcaccgccg ccggctccgg ctgcatggcc gcgctggacg ccgagcgcta
    12481 tctcgaggct acccaggccc actgaggcgg gcgccgggcg agccgcgcgg catggcgtgc
    12541 cgcgtcggtc ccgatggcgc tcgcgccccc cgcaggcggc ggagccctcg cggcggagcg
    12601 tccccctcgg gccgagtcgc gatttccccg ggacgcgcaa cgccgtcgtc cgctcgacat
    12661 cccgaggccg accggtgggc gatcgttcag tccttcgggc cccgcgatgt ctggacgctg
    12721 aaccggcgac gtcaggtgcc gacgcggagg caggcgcgga gctcgcggag cagggcgacg
    12781 gtggagaggt aggagagctt gccggtacgc gggttctcgg aaggcacgtt ctcgatctcg
    12841 atggtgaagg tgccgaactc gccctccgcg cgcacgcggt ggcggttcat cgcgaggccg
    12901 ggcacggcgt agatcctcgt tcgcgtgcgc tcgggcccga tgccggcgag cgagacggcc
    12961 gctagcacgt tgacattggc ggggaaagcg cgacaggcgt cccgcgccgg cccctcgaag
    13021 acgagggtct cctcgcggat cgcgtcgagg tcgatcttgt tctccaggat ccacggcgcg
    13081 ccggccaggc ctcgcggcgg cttgcgggtc tccatgacga ccgacgtcat cgcgccggtg
    13141 cgtgcgccct tgaggccgtc gagccccgcg atcgccccgg acaccacgtg gatccgcgcg
    13201 cgcttggcct cggccagggc tacccactcg gggtggtcga ggagcgcgcc gcaggagagc
    13261 acgacgaggt cccggcccgc ggccagcgcc accggggcga cctcctcgag gtgggcccgg
    13321 gtggaggcct cgacgaggat gtccgaggcg gcgatcaggt cgcccagggg caggaagggc
    13381 ggctcgacgt ccagggtccg gagaaacgcc tcggccttga gccggtcgcg cgcgagcgcg
    13441 ccggccaggc gaagccccgg gagcccggcg tccagcgcgc ggcacaccgc gcggccgatg
    13501 acgccgagac cgaggacgcc gacgcttcgc tgcgtgtcca tcgcgaaacg cgcgcatgat
    13561 atcatgccgc cagagcggcg aatgacgagc cccagcgcct tgcgcgaacc gctcgagcgc
    13621 ctgtaccgcg agttcgacta cgccgcccgg gtcgagcgcg acgccatccg gttccccctc
    13681 cgctacgacg atccgcggga ccgcgagatc gtcgcgctcc tgaccgcgtg cctggcctac
    13741 gggcgggtcg atctgttcgg ccgcgagctg gaccgcgtgc tgaccgccat ggggccgtcc
    13801 ccggcggcct tcgtcgcggg cttcgatccg tgtcaccacg cggccacgtt cgcggccttt
    13861 cgctatcgct tcaaccgccc gcgcgacctg acggcgttct gcgtggccgc gcgcgacctg
    13921 ctggcccgtc acggcacgct cgagaagctc ttcctcgccg gggatccgga cgcgtgtggt
    13981 cccgttggcc cggcgctcga gcgcttcgcg cgggccttcc tcgacgccga tctctcggac
    14041 gtcttcccgc gccgccggct gtcgcgcggg taccgccacc tgttcccggt gccctccgcc
    14101 ggagggccgt gcaagcgcat gcatcttttc ctccgctgga tggtgcggcg cgagccgccg
    14161 gacttcgggc tgtgggcctc ggtgtcgccg gcgcgcctgc tgatccccgt ggacacgcac
    14221 atcgagaaca tgagccgggc catcgggctg acgcgacggc gctcgcgcaa ctggcgcatg
    14281 gccgaggaga tcacggcgcg cctggccctg gtcgatcccg ccgaccccgt gaagtacgac
    14341 ttcgccctct gccacaagcg catgtcgggc ggctgccgcg atcggcgcga cccggtgatc
    14401 tgcaccccct gcggcctccg atcggtatgt cgacactggg cgaagccgct gcgccagcca
    14461 cgatcatgag cgagacggtg atcgcgctcg tgggcctggt cgtcggaggc gcggccgcgt
    14521 gggtcctcgc ccgcgcccac ttccggagcg tggccctcgg cgaggcgcag gcgcgcgagt
    14581 cacgcctggc cgtggccgaa agcctcgccg acgagctgcg caagcagctc tcccatcgcg
    14641 atctggaggc tggcgacctc cgcagcaccc tgcaggccga gcgggccctc cgcgcgcagg
    14701 cggaggcgcg cgccgagtcg acgcgcgaga acctcgagga gcagaagcgg ctcctcgagg
    14761 gcgcgcggga gcggctgacg gaaaccttca aggcgctctc ggcggacgcg ctccggcaga
    14821 gcacgtcggc gttcctcgag caggcccgtg agacgatcga cggtcagctc gcgcgccgcc
    14881 aggaggccct cgacggcgtc gtcaagccgc tccaggagtc gctcaagcga tacgaggcgg
    14941 agctgcgcca gctcgagacc tcgcgtcagc aggcgtacgg cagcctcgag gagcagctca
    15001 agaccctcaa tgcgcgcagc gccgagctcc agcgcgagac cggaagcctg gtgacggcgc
    15061 tgcggtcgcc ccaggtgcgt ggacgctggg gcgagctcac gctgcggcgc gccgccgagc
    15121 tggcggggat gacgccgtat tgcgactatt tggagcaggt gacggtcgag ggcgagggcg
    15181 gccggctccg gcccgacatg atcgtccgcc tgcccagcgg ccggacgatc gccgtggacg
    15241 cgaaggtgcc cctcaacgcc tacctcgacg cgacgggggc ggtaaccgag gacgagcgcg
    15301 gccgcttgct ggggcgccac gcccagcagg tccgccagca cctcgccgtg ctctcggccc
    15361 gcgcgtactg ggaccagctc gatgactccg tcgagctggt ggtgatgttc attcccggag
    15421 aggcgttcgt cggcgccgcc atgcaggcgg acgcccggct gctcgaggac ggcatggcca
    15481 agaagatcct catcgccacg ccgaccacgc tgatcggcct gctgctggcc atcgcgcacg
    15541 gctggcagca ggagcggctc gccgtcaacg ccgcccagat cagcgagctg ggccgcctgc
    15601 tctacgagcg cctgcggacg ctggccggcc acgtcgagga cgtcggagac aagctcggca
    15661 aggccgtcca cgcctacaac gccgcggtgg ggtccatgga atcccgcgtg ctgccggcgg
    15721 cgcgccggtt ccgcgacctg ggggcggccg gcggggagga gattccggtc ctcgaggcgg
    15781 tggacgagcg cccgcgctcg ctgaccgtgc ccgacgtgcc cgagcagctt cgcgcccccg
    15841 acctgcccgc ctgaccgccc gcgacggcgg ccgccggccc cgcgctactt gaggctgccc
    15901 gccgtcagcc cgcccacgag atagcgctcg atgagcgcga agagcacgac gaccggcgcg
    15961 atggcgatga ccgaggcggc gaagagcagg ttccagtgct gcacgtaggc cgtcacgtag
    16021 gtcgtgatcc ccacggtcag cggcttcatg tcggggtccg tcatgagcgt cagcgcgacg
    16081 atgtactcgt tccaggcgcc gatgaaggcg aagatcacgg cggtcacgat gcccgggagc
    16141 gacagcggga gcacgatccg ggcgaggcag cgcacccggc cgcacccgtc cacggcggcg
    16201 gcctcctcga tctcgcgggg aatggaggcg aagaacgctc gtaggatcca gatggcaaag
    16261 gccaggttga aggcggcgtt ggtgaggatc agcgcggcgt aggtgttgac gaggtcgagc
    16321 tcgaaaaatt cgcggtagag gccgaccacc agcgctgtcg gcgcgaacat ctgggtgacg
    16381 aggacgacga acaggaacgc ggtgcggcca gggaaccggt gccgcgcggt gtagtaggcg
    16441 gccggcaccg cggccaggat cacgagcgcc gtcgaggcca gggccaccac gagcgagacc
    16501 tgcagccagg attgaaaggc cgggtcgcgc agcacctcga ggaaattgcc cggctgccag
    16561 tcgagcgggg tgaggcgcgg cgggctcgtg cgcagctcgc gatccggctt cagcgcggtg
    16621 aagaacatca ccagataggg tgacaggacg gcgccgccga ccagcgcgcc ggccacggtc
    16681 agccatggtc gcccgatcgc gcggctcatc gccccggttc ggtcacgcct cctgctcgcg
    16741 ccagcgcacc attttcacgt acaccaccac ggcgagcagg atcaccagca cgttgaccac
    16801 cccgagggcc gccgccagcc cgacgtcgcg cagcgcgctc ttgaaggcga tcttgtacat
    16861 gtacgtgacc atcgtgtcgt ggccgaatcc gggattgcgg tcattgagcg tccacacgat
    16921 ggggaacgag ttgaagacgg agatgatgtt caggaccgcg gccaccagca gggctggccg
    16981 gagcagaggc agggtcaccg agcggtatgt ccgccagagg gaggcgccgt ccacgcgggc
    17041 ggcctcgtac acatcgccgg gaatggtttg cagcccggcc aggaacacgt acgtcgtgaa
    17101 cgggagcgag acgaagaccc ccacggcgat catggcgccc atcaccgtgg catcgtcgcc
    17161 gagccagtcg aggggatgcc cgatcagcga gagcgacagc agcacgtggt tgaggatccc
    17221 gaagtagtag tcgtagatcc acacgaacag cttcgacgtc atgatcagtg acgccgccca
    17281 cggcacgatg agggcccagc ggaccagccg ccgcccggga aagctctggt cgaggagctg
    17341 cgcgagcccg agcgagatca cgatggtgac ggcgacgacg accgtcaccc agatcattgt
    17401 gttcgccagc accgcgggca gcgcatcctg ccggaagagc gcggcgtaat ttcgggtgcc
    17461 caccgagccc aggtagaggc cggtgacgga gtagcggctg agcgaggcag cgccgagctg
    17521 caccgcgggg tacaccacga ctgccgcgat cagtgccagg ctgggcccga gccagaggag
    17581 cgccgcccat cccgccggcc ggtgccggga accttggcgg gacgtcgagg cgtgccacgg
    17641 cgtcgccgga gcgtgccgag ggtgggaagc tatttccccg ccccccacgt cgtcgtccgc
    17701 tcgcacggga tcgagccggc cggctcgccc gtcagcgggc gctcgccgcg gccgtggcgc
    17761 gctgctggag ctgatcgagg acctgcttcg ggttgccccc gggctgcacc gccaggccga
    17821 tggtctgctg gacgtcgagc ttcaccttgt cccaggccgg atcggtcgtg ggcacgagcc
    17881 tcgcgttcgg cagggcggca aggtacgggg cgagcctggg attgccgctc atctgcgtga
    17941 gtccggacct ggtcacaggc aggaagccct cggcggtgat ccaccgggtg acgttccgtg
    18001 gctcgtagta gagatcgagg aactgccgga ccgcctcctg gttgcgcttc ttcctgaagg
    18061 ccatcaggta gtcctcgacg cctagcgtga cggccgtgcc cacgttggtg ggcatctcgg
    18121 ccacgccgta gtcgaccttg ccctcggcgt cgagctgggc cgctagcggg ctgaagccca
    18181 tcaccatgcc gaccttgccg tccttgaaga gctggaaggc gccgtcggtg cggttggtct
    18241 tcccggggtt gacctgggtc accttgtgcc ggtgcgcgag gtcggccaga aactgcaggg
    18301 tctgcacgtt cctgtcgctg ttgatcgccc acacgcctcc ggccttccag tcgccgccgt
    18361 tgttccacat ccagatcgac cactccgcct gcgcctcctc gggaccgagc ggcagggcgt
    18421 agccgatcgt cccctcgccc agggcc
//
LOCUS       DATIAZ010000113         2875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_685808, whole genome shotgun sequence.
ACCESSION   DATIAZ010000113 DATIAZ010000000
VERSION     DATIAZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685808"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VLF19_03920"
     CDS             <1..199
                     /locus_tag="VLF19_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid carrier protein IgrF"
                     /protein_id="HSE92430.1"
                     /translation="HGMSMVIENVRQLRGTVDDYCPRAAEGVHTYDYAPGRCRQVKDA
                     EITMNLGWAMPPTGSALILRR"
     gene            300..806
                     /locus_tag="VLF19_03925"
     CDS             300..806
                     /locus_tag="VLF19_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSE92431.1"
                     /translation="MPQPVQYRGMTLIVPDNDSEWMEFYQHARAHRLVLRRCSACGLM
                     RYPPSHACPWCMALEWTWQEVGGRGTIYSYEVVAHAIQPGFKDATPYAVVLVELDEQR
                     GQPTPDEALRIVANLVKADGSMEDESQVAIGRRVQVVFHDLADHLALPQFRLTDEAPQ
                     GRVWRLPG"
     gene            complement(882..2450)
                     /locus_tag="VLF19_03930"
     CDS             complement(882..2450)
                     /locus_tag="VLF19_03930"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSE92432.1"
                     /translation="MEDRTAILPDARRPAAATEADVPRDLREWLARAEAVGQILRIRP
                     TVARDEMGAITYMAHQTIGAPAPLFESIEGCPRGFRALWNLIGSSPDRFALAIGEPTG
                     LTVMELIRRCKGRFTQSLAPVFVDPSQAPVNDHHVLGDDVDVTIFPAGRHWPRDGGPY
                     IGTADAVITRDPDAGWLNVGCYRQMVHDGKHVGLYLSPGKDARLHIERYWSRGEACEV
                     VAVWGVDPAMFIAASLTFPKTVSELDFIGGITGRPVELCKGQAGSVPYPARAEIVIEG
                     VVRPEALRREGPFGEFTGYYGRPEDLAFDVEVKAIHHRDAPILTHALMADYPANETSL
                     LYSIARSAKIWNDLDRLGVPGIHGVYSHPAAAGGWGMTVVSLEQRYAGHAPQALALAA
                     QCPGGAYYAKWIIAVDEDVDPSNTNQVLWAMATRCNPVEDIDILRQTWSTWLDPTQNP
                     PEERPYGSKALINACMEHRHIRQFSKRTKVRRSVYESVARRWAELGFTGAPPRLWSLE
                     DSSEGAGGSQGGAR"
     gene            2692..>2875
                     /locus_tag="VLF19_03935"
     CDS             2692..>2875
                     /locus_tag="VLF19_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92433.1"
                     /translation="MKSIVLAIGILAALGVTAAGAQDLDLEAPAPLREPRVPVPEPGP
                     GPSVPYDPAFIRPFTID"
BASE COUNT          420 a         1028 c          991 g          436 t
ORIGIN      
        1 gcacggcatg tcgatggtga tcgagaacgt gcgccagctt cgcggcaccg tggacgacta
       61 ctgcccccgg gctgccgagg gcgtgcacac ctacgactac gctccgggcc gctgccgtca
      121 ggtcaaggac gccgagatca cgatgaacct cggctgggcc atgccgccca ccgggagcgc
      181 actgatcctg cgccggtgac acgacgcgtg agctcgtgtt cgccacggtc gatcgaccac
      241 ggctccgagg gccgctgcgc gtccgtcctc aactccgccg gccccgcgag gacatcctga
      301 tgcctcaacc ggtgcagtac cgtgggatga cgctgatcgt ccccgacaac gacagcgagt
      361 ggatggagtt ctaccagcat gcccgcgccc accggctggt cttgcggcgc tgcagcgcgt
      421 gcgggctgat gcgctacccg ccgtcgcacg cctgcccttg gtgcatggcg ctcgagtgga
      481 cgtggcagga ggtcggcggc cgcgggacga tctactcgta cgaggtcgtg gcgcacgcga
      541 tccagcccgg cttcaaggac gcgacgccct acgcggtggt gctcgtcgag ctggacgagc
      601 agcgcggcca gccgacgccc gacgaggcgc ttcgcatcgt cgccaacctc gtcaaggccg
      661 acggctcgat ggaggacgag agccaggtgg ccatcggccg gcgcgtgcag gtggtctttc
      721 acgacctcgc cgaccacctc gccctgccgc agttccggct caccgacgag gccccgcagg
      781 gccgcgtctg gcgcttgccc ggctgagcag ccgaaggaac ccgcggggag cccgagactc
      841 cttccggcgg gcaaaccggc tggtcgatcg tgccggttgc gctatcgcgc acctccttgc
      901 gagccgccgg ccccttcgga ggaatcctcc agcgaccaga gccgtggcgg ggcgcccgtg
      961 aagcccagct cggcccatcg ccgggccacc gattcgtaga cggagcggcg gaccttcgtg
     1021 cgcttcgaga actgcctgat gtggcggtgc tccatgcatg cgttgatcag cgccttggag
     1081 ccgtagggtc gctcctcggg cgggttctgg gtggggtcga gccacgtcga ccacgtctgc
     1141 cggaggatgt cgatgtcctc gacgggattg cagcgcgtgg ccatggccca cagcacctgg
     1201 ttggtgttcg agggatcgac gtcctcgtcc accgcgatga tccacttcgc gtagtacgcg
     1261 ccgcccgggc actgcgcggc cagcgcgagc gcctgaggcg cgtgccccgc gtagcgctgc
     1321 tccagcgaga cgaccgtcat cccccacccc ccggccgccg cggggtggct gtagacgccg
     1381 tggatgccgg gcacgcccag ccggtccagg tcgttccaga tcttcgccga gcgcgcgatg
     1441 gagtagagca ggctcgtctc gttggccgga tagtcggcca tcagcgcgtg ggtgaggatg
     1501 ggagcgtcgc ggtggtggat ggccttcacc tcgacgtcga aggcgaggtc ctcgggacgg
     1561 ccgtagtagc cggtgaactc gccgaacggc ccctcgcgcc ggagcgcctc ggggcgcacc
     1621 acgccctcga tcacgatctc ggcgcgcgcc ggatagggca cgctgcccgc ctgacccttg
     1681 cacagctcca ccggccgccc ggtgatcccg ccgatgaagt ccagctcgga gacggtcttg
     1741 ggaaacgtca gcgaggccgc gatgaacatg gccggatcca cgccccacac ggcgaccact
     1801 tcgcacgcct ccccccggct ccagtaccgt tcgatgtgca ggcgcgcgtc tttgcccggc
     1861 gacaggtaga ggccgacgtg cttgccgtcg tggaccatct gccggtagca gcccacgttg
     1921 agccagccgg cgtcgggatc gcgcgtgatg acggcatccg ccgtcccgat gtaggggccc
     1981 ccatcgcggg gccagtgccg gccggccggg aagatcgtca cgtcgacgtc gtcaccaagc
     2041 acgtggtggt cattgacggg cgcctggctc ggatccacga agaccggcgc caggctctgc
     2101 gtgaaccggc ccttgcatcg ccggatcagc tccatcaccg tgagcccggt cggctcgccg
     2161 atggcgagcg cgaaccggtc cgggctcgag ccgatcaggt tccacagcgc gcggaacccc
     2221 ctcgggcacc cctcgatgct ctcgaacagg ggcgcgggcg cgccgatcgt ctggtgcgcc
     2281 atgtaggtga tggcccccat ctcgtcccgc gcgaccgtcg ggcggatccg aaggatctgg
     2341 ccgaccgctt cggcccgggc cagccactcg cgcagatcgc gcggcacgtc ggcctcggtg
     2401 gcggcggccg gtcggcgcgc gtcgggcagg atcgcagtgc ggtcttccat ggggggcacc
     2461 tccaccaggc gacgagaccg ttcacggccg gggaaacgtc caggtaggct actccagcgc
     2521 gccgcgggaa ctcaatccct ccgagccgga agtcgaaggg gcggcggcaa cccaccatgg
     2581 gacgatcgtg ccccggcctc gtcctacgac gcccctcgcc aaaccgcgtc gtgattgatg
     2641 ttcgggccag ggccgaagtg gtacggccca tgcatttcgg agctcgacat ggtgaaatcg
     2701 atcgtgctcg caatcgggat cctggctgcg ctcggcgtga cggcggccgg cgcccaggac
     2761 ctggacctcg aggcgcctgc gccgcttcgc gagccccgcg ttcccgttcc cgaaccgggt
     2821 cctggcccga gcgtgcccta cgatcctgca ttcatccgtc cgttcacgat cgacc
//
LOCUS       DATIAZ010000114         4732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1211934, whole genome shotgun sequence.
ACCESSION   DATIAZ010000114 DATIAZ010000000
VERSION     DATIAZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211934"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VLF19_03940"
     CDS             complement(<1..763)
                     /locus_tag="VLF19_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSE92434.1"
                     /translation="MTRLFGTDGIRGVANEEPLTAELAYRIGRELVATLHDEGHVERV
                     RLVIGRDTRRSGPLLEAAITAGVLSAGGECYAVGVMPTPAIALLTRALEAHGGIVLSA
                     SHNPFEDNGIKLFSSEGTKFPEAWEDDIEAGLARDDGAARARGADIGRHATYDRAETY
                     YVDFLCRCFPLDLGGFTIALDCAHGATYRVAPSVFRRLGARVIVTSAGPDGTNINAGC
                     GALHPEGLRKRLVAAGADVGFAFDGDGDRLISVDHL"
     gene            complement(818..1729)
                     /gene="folP"
                     /locus_tag="VLF19_03945"
     CDS             complement(818..1729)
                     /gene="folP"
                     /locus_tag="VLF19_03945"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSE92435.1"
                     /translation="MTSNVADLAGVGVGAGWPVVVMGAVNVSPESFYAGSVRRDAAAL
                     LDAARKMVDAGAAVIDVGARSTAPYLAAAITDDEETARLSRAVEALAAELPVPVSADT
                     ASVRGARAAMDAGAQIVNDVTGLRDPAMAALVHARARGVILMASPDGVRGASPSATPR
                     STRGRASEGARTADPVERVRALLRQALERAQAAGIADRRIVVDPGIGFFRDEGLSWDE
                     WDVRVLAGLERLQSLGRPLGVAVSRKSFVGAITGRARPEDRLAGSLGAAAVAVVHGAA
                     LVRAHDVAQTVDAVRVAERLRPARRRC"
     gene            complement(1822..3639)
                     /gene="ftsH"
                     /locus_tag="VLF19_03950"
     CDS             complement(1822..3639)
                     /gene="ftsH"
                     /locus_tag="VLF19_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSE92436.1"
                     /translation="MNQVYKNLALWMVIGLIVILLFTVFQGAQQGGQEQPNFSEFLRA
                     VEQSRVESVVIRGNLVTYTLKDSSQTHRTYIVDYPDLIKMLRERGVRIAVKPPDSNPW
                     YAIFLQWVPMLLFIGVWIFFMRQMQGGGAKALSFGKARARLISEKQNKVTFQDVAGVD
                     EAKEELREIIDFLKDPQKFQKLGGKIPKGVLLVGPPGTGKTLLAKAIAGEANVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFMDEIDAVGRHRGAGLGGGHDEREQT
                     LNQLLVEMDGFETNEGVILIAATNRPDVLDPALLRPGRFDRQVVVPRPDVKGREEILR
                     VHARRIPLAPDVDLKVLARGTPGFSGADLANLVNEAALLAARLNKKFVEMLDFENAKD
                     KVLMGVERRSMIISDTEKKTIAYHEAGHALVADFLPGTDPVHKVTIIPRGRALGLTQQ
                     LPMDDKYNYSREYLINRITILLGGRAAEEIVLNQQTTGAGDDLEKATEMARKMVCEWG
                     MSDRLGPLTFGKNEEHIFLGREVARQKDYSEQTALLIDSEIKRIVIDCATRARQILDE
                     NIEKLHALARALLERESLDGEEIARILRARPFSEAPAPA"
     gene            complement(3694..4332)
                     /locus_tag="VLF19_03955"
     CDS             complement(3694..4332)
                     /locus_tag="VLF19_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSE92437.1"
                     /translation="MSARRAARQESRAASFFLLLGCLTVLGGAFTGGVFAGRQWPGLL
                     PSIGNPREAALRASGKPPSGRPAPAGPAGPTARPAEPPPVLTFYQELTAPLTAPPPAP
                     KPKPVERAPRAESLRIDSATVAGESAKVERASAERRFSVQVGAFRTREQADAIRTKLV
                     SAGHDAYITEGERSTGTRYRVRAGSFTSREEALRAAERIGAAVKLATYVTIR"
     gene            complement(4329..>4732)
                     /locus_tag="VLF19_03960"
     CDS             complement(4329..>4732)
                     /locus_tag="VLF19_03960"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HSE92438.1"
                     /translation="ATRQSAENPVYYVQYAHARIRSLWRQAAEQGIRPPAWRDVDLGA
                     LARPEELALIKRLVQFPDLVAGAARSLEPHRIAFWLTELAGQFHPYYKAHRVIQADER
                     LMFARLGLCTAVGQVVANGLGLLGVSAPESM"
BASE COUNT          668 a         1704 c         1664 g          696 t
ORIGIN      
        1 cgaggtgatc gacggagatc aaccggtcac cgtcgccgtc gaaggcgaag ccgacgtccg
       61 cgccggcggc cacgagtcgc ttgcgcagcc cctcggggtg gagcgcgccg cacccggcgt
      121 tgatgttggt gccgtccggc cccgccgagg tgacgatgac gcgcgcgccc aggcggcgga
      181 agacgctcgg cgccacgcgg tacgtcgcgc cgtgggcgca gtccagcgcg atggtgaagc
      241 caccgagatc gagcgggaag caccggcaca ggaagtcgac gtagtaggtc tcggcgcgat
      301 cataggtggc gtgccggccg atgtcggcgc cgcgcgcgcg ggccgcgccg tcgtcacggg
      361 cgagccccgc ctcgatgtcg tcttcccacg cctcggggaa cttcgtcccc tcggacgaga
      421 acagcttgat gccattgtcc tcgaacgggt tgtgggaggc cgagagcacg atgccgccat
      481 gcgcctcgag cgcccgcgtg agcagggcga tggcgggggt gggcatgaca ccaacggcgt
      541 agcactcgcc acccgcgctc agcacgccgg ccgtgatcgc cgcctccagc agggggcccg
      601 agcgccgggt gtcgcgtccg atcacgagac gcacccgctc cacgtggccc tcgtcgtgca
      661 gcgtggccac cagctcgcgc ccgatccggt aggcgagctc ggccgtcaac ggctcctcgt
      721 tggccacacc gcggatcccg tcggttccga acagccgcgt catcccgctc ccttcaccct
      781 tcgcacttcg attgtagacc gggcccgcgc atttccgtca gcaccgccgc cgcgctggtc
      841 gcaagcgctc ggcgacgcgg acggcgtcca cggtctgggc cacgtcgtgc gcgcgtacca
      901 gcgccgcgcc gtgcaccacc gccacggccg cggcgcccag cgagcccgcc aggcggtcct
      961 cgggccgggc gcggccggtg atggcgccca cgaacgactt gcgcgagacg gcgacgccca
     1021 gcggtcgtcc cagcgactgc agccgttcga gacccgccag gaccctgacg tcccactcgt
     1081 cccacgacag gccctcgtct cggaagaatc cgatgcccgg gtcgaccacg atgcgccggt
     1141 cggcaatgcc ggcggcctgg gcacgctcga gagcctgtcg gagcagcgcc cggacgcgct
     1201 cgacaggatc cgcggtccgg gcgccttctg aggcccggcc cctcgtggac cgcggggtcg
     1261 ccgacggcga cgccccccgc acgccgtccg gcgaggccat caggatgacg ccgcgggcgc
     1321 gagcgtggac gagggccgcc atggccgggt cgcgcaatcc ggtgacgtcg ttgacgatct
     1381 gcgcgccggc gtccatcgct gcacgcgcgc cgcgcacgga ggccgtgtcc gcggacacgg
     1441 gaacgggcag ctccgccgcc agcgcctcca cggcgcgaga caggcgtgcg gtctcctcgt
     1501 cgtccgtgat tgccgcggcc aggtacggcg ccgtcgagcg cgcgcccacg tcgatgacgg
     1561 ccgcgccggc gtccaccatc ttgcgcgccg cgtccagcag ggcggccgcg tcccgtcgga
     1621 ccgagcccgc gtagaacgac tcggggctca cgttgaccgc ccccatgacg acgaccggcc
     1681 acccggcgcc gacgcccacc ccggcgaggt cagcgacgtt ggacgtcatg gcaggcgatc
     1741 cggtcgcgcg gtccgcgcgg tcgaggcgtg acagctgcgc ccccgtgggg catcactcga
     1801 ggtcggtggg cgagcgcgac gctacgccgg cgccggcgcc tccgagaagg ggcgggcgcg
     1861 aaggatgcgc gcgatctctt cgccgtcgag cgactcgcgc tcgaggagtg cccgcgcgag
     1921 ggcgtggagc ttctcgatgt tctcgtcgag gatctgccgc gcccgggtgg cgcagtcgat
     1981 gacgatacgc ttgatctcgc tgtcgataag cagggccgtc tgctcgctgt agtccttctg
     2041 gcgcgccacc tcgcggccga ggaagatgtg ctcctcgttc ttgccgaagg tcagcggccc
     2101 gagcctgtcc gacatccccc actcgcacac catcttgcgg gccatctcgg tcgccttctc
     2161 gaggtcgtcg ccggcgccgg tggtctgctg gttcagcacg atctcctcgg cggcgcggcc
     2221 gcccagcagg atcgtgatcc ggttgatcag gtactcgcgc gaatagttgt acttgtcgtc
     2281 catcggcagc tgctgcgtga ggccgagcgc gcgaccgcgc gggatgatcg tcaccttgtg
     2341 cacggggtcc gtaccgggca ggaagtccgc caccagcgcg tgacccgcct cgtgataggc
     2401 gatggtcttc ttctcggtgt cgctgatgat catggagcgc cgctcgacgc ccatgaggac
     2461 cttgtccttg gcgttctcga agtcgagcat ctccacgaac ttcttgttga gccgcgcggc
     2521 cagcagcgcc gcctcgttga cgaggttcgc gaggtccgcg cccgagaagc ccggggtacc
     2581 gcgggccagc accttcaggt ccacgtcggg ggccagcggg atccgccgcg cgtgcacgcg
     2641 aaggatctcc tcgcgtccct tcacgtcggg tcgcggcacc acgacctgcc ggtcgaagcg
     2701 gccagggcgg agcaacgccg ggtcgaggac gtcgggccgg ttcgtcgcgg cgatgaggat
     2761 cacgccctcg ttcgtctcga acccgtccat ctcgacgagg agctggttga gcgtctgctc
     2821 gcgctcgtcg tggcctcccc cgaggcctgc gccgcggtgg cgacccaccg cgtcgatctc
     2881 gtccatgaag atgatgcacg gcgcgtgctt cttgccctgc tcgaagaggt cgcgcacgcg
     2941 cgaggcgccc acgcccacga acatctcgac gaagtcggag ccagagatcg agaagaacgg
     3001 cacgttggcc tcgccggcga tggccttggc cagcagcgtc ttgccggtgc cgggcgggcc
     3061 gaccagcagc acgcccttgg ggatcttgcc gccgagcttc tggaacttct gcgggtcctt
     3121 caggaagtcg atgatctccc gcagctcttc cttggcctcg tccacgccgg cgacgtcctg
     3181 gaacgtgacc ttgttctgct tctcggagat gagccgcgcg cgggccttgc cgaacgacag
     3241 cgccttggca ccgccaccct gcatctggcg catgaagaag atccagacgc cgatgaacag
     3301 gagcatcggc acccactgca ggaagatcgc gtaccagggg ttcgagtccg gcggcttcac
     3361 cgcgatccgt accccccgct cccgcagcat cttgatcagg tccgggtagt cgacgatgta
     3421 ggtccggtgc gtctggctgg aatccttcag cgtgtaggtg acgaggttgc cccggatgac
     3481 caccgactcg acgcggctct gctcgacggc acggaggaac tcggagaagt tcggctgctc
     3541 ctggccaccc tgctgcgccc cctggaagac ggtgaaaagg aggatgacga tcaagccgat
     3601 caccatccag agggcgaggt tcttgtagac ctggttcatg tggcctcgat tatagcacca
     3661 ggcatgccat gacgcgccgc ggcaaccgcg atctcagcgt atcgtcacgt aggtcgccag
     3721 cttgacggcg gcgccgatgc gctcggcggc ccgaagcgcc tcctcgcggc tcgtgaacga
     3781 gccggcccgc acgcggtacc gggtgccggt cgaccgctcg ccctcggtga tgtaagcgtc
     3841 gtggccggcg gagaccagct tcgtgcggat ggcgtccgcc tgctctcgcg tccggaacgc
     3901 gcccacctgc acgctgaagc gccgctcagc tgacgctcgc tcgaccttcg cgctctcgcc
     3961 cgccaccgtc gcgctgtcga tccgcaggct ctcggcccgc ggcgctcgct cgacgggctt
     4021 cggcttcggc gcgggcggcg gcgccgtcag cggcgcggtc aactcctgat agaaggtgag
     4081 gactggcgga ggctcggccg gccgggccgt cgggcccgca ggtcctgccg gtgcggggcg
     4141 gcccgaaggc ggcttgccgg aggcccggag cgccgcctct cgcgggttgc cgatcgacgg
     4201 tagcagtccg ggccactgcc ggcctgcgaa gacgccgccg gtgaacgcgc cgcccagcac
     4261 ggtgaggcag ccgagcagga ggaagaagga cgcggcgcgg ctctcctgcc gtgccgcacg
     4321 tcgggcgctc acatgctctc cggggcgctc acgccgagca ggccgagccc gttcgcgacc
     4381 acctgcccca ccgccgtgca cagccccagc cgcgcgaaca tgagccgctc gtcggcctgg
     4441 atcacgcgat gggccttgta gtacggatgg aactgccccg cgagctcggt gagccaaaac
     4501 gcgatccggt ggggctcgag cgagcgcgcg gcgccagcga ccaggtccgg gaactgcacg
     4561 aggcgcttga tgagcgcgag ctcctcgggc cgcgcgagcg cgccgaggtc cacgtcgcgc
     4621 cacgccggcg gccggatgcc ctgctcggcg gcctgccgcc agagcgagcg gatgcgcgcg
     4681 tgcgcgtact ggacgtagta gacggggttc tccgcggact ggcgcgtggc ca
//
LOCUS       DATIAZ010000115         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1212043, whole genome shotgun sequence.
ACCESSION   DATIAZ010000115 DATIAZ010000000
VERSION     DATIAZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212043"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..877)
                     /locus_tag="VLF19_03965"
     CDS             complement(11..877)
                     /locus_tag="VLF19_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSE92439.1"
                     /translation="MLRGLILIALAAVSWGTTGSVTTVLVATANAEPLLIGAARTVVA
                     AALLLIGARVVAGGLTVARADRGRCVAAGVGMAVFQVAYFTAVTLSGIAAAALVAICT
                     APLMIAALAALFLGERVTGRVAAALFLGVAGTALLVAGPTEGAPPARFAGGVLLALVA
                     GLAYALYAVLTKASLARSAPLPLTAATFAVAAACLVPALAWTERPLTQVALGWPWLLY
                     LGAVTTAGAYALYAAGLRMVPAAVAGVVTLLEPLTATLLAVLVFGERLGLTGAIGAAL
                     LLSAIALLSRRP"
     gene            1020..1922
                     /gene="mmsB"
                     /locus_tag="VLF19_03970"
     CDS             1020..1922
                     /gene="mmsB"
                     /locus_tag="VLF19_03970"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HSE92440.1"
                     /translation="MKIAFVGTGTMGQPMVGNLVKKGHAVVAHDVVPAALDAATKLGA
                     TRAASAAEAARQAEMVVTMLPSSPHVEAVYLGAGGILEGVARGRLCVDMSTIEPAVSR
                     RVAEVARQRGVRFVDAPVSGGVPRAEDGTLAIMVGGEARDVEEARPILAAMGSTVIHV
                     GPVGAGEVAKLCNNLIAGVAAVAVSEAFRIAEGFGVDPKILTEVIAKSSGHTWLMEHM
                     HPVPGLVPRSASSRDYAPGFMTDLMAKDLGLAVNAARTLRVPVFVAPAAQQVLRLASS
                     HGLGRKDFTSVYQFLRPSDGAAPV"
     gene            1966..2286
                     /locus_tag="VLF19_03975"
     CDS             1966..2286
                     /locus_tag="VLF19_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSE92441.1"
                     /translation="MSLYIMLSTLSESGRKVLRGRPGWIRKVNADVQRMGARVVAQYA
                     VLGPYDFVTVIEAPDNATISRVSVELGARGGVAGMTMAAIPLEEFITRLERSGNKRST
                     RKKR"
     gene            2283..2753
                     /locus_tag="VLF19_03980"
     CDS             2283..2753
                     /locus_tag="VLF19_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSE92442.1"
                     /translation="MRVLKLIPERCTGCLRCELACSYMQTGEFKPAKSVIRVSPFEGH
                     TSYAPYTCTQCAEGWCMTACPVGAIVISAVGAKDVVDDRCVGCKLCTIACPYGTMFYD
                     PDTRKAFKCNLCGGDPACAHACPTAAILYEETATVDWLGEFAAARTSRLLAGVS"
BASE COUNT          429 a         1016 c         1080 g          359 t
ORIGIN      
        1 cggaacgtcg tcacggacgc cgggacagga gcgcgatcgc ggacagcagt agggccgcgc
       61 cgatcgcgcc ggtgagtccc aggcgctcgc cgaagacgag gacggccagc agcgtcgcgg
      121 tcagcggctc caggagcgtg acgactcccg cgaccgccgc cggcaccatt cgcaggcccg
      181 ctgcgtagag cgcataggcg ccggccgtgg tcaccgcgcc gagatagagg agccacggcc
      241 accccagcgc cacctgagtc agcgggcgct cggtccatgc cagagccggc accagacagg
      301 ccgccgcaac ggcgaacgtg gcggcggtga gcggcaaggg cgcgctgcgg gccaggctcg
      361 ctttcgtgag gacggcgtag agcgcgtagg cgagccccgc gacgagcgcc agcagcacgc
      421 ccccggcgaa ccgcgcgggc ggcgcgccct cggtggggcc ggcgacgagc agggccgtgc
      481 cggcgacgcc gaggaagagc gccgcggcca cgcgcccggt gacgcgctcg cccaggaaca
      541 gcgcggccag cgcggcgatc atcagcggcg cggtgcagat ggccacgagc gcggcggccg
      601 cgatgccgct cagcgtcacc gccgtgaagt acgcgacctg gaagaccgcc atgccgacgc
      661 cggcggcgac acagcgtccg cggtcggcgc gcgcgacggt caggcccccc gcgaccacgc
      721 gcgcgccgat gagcaggagc gcggcggcga cgaccgtgcg cgcggcgccg atgagcagcg
      781 gctcggcgtt cgccgtcgcc acgagcacgg tggtgaccga gccggtcgta ccccaggaca
      841 ccgcggccag cgcgatcagg atcaggcccc gcagcacggc gcgggattat agcggcccgg
      901 gcgacacgcg gcttgaccgc ggcgcgcggc cccggggatg atagccgggg ttttcccggg
      961 gaggccgaga tgatgatgga aggacgccgc gccggcggcg atccggaggg tcagcagcca
     1021 tgaagatcgc cttcgtcggc acgggcacca tggggcagcc catggtcggc aatctggtca
     1081 agaaggggca cgcggtcgtc gcccacgacg tggtgcccgc ggccctggac gccgcgacga
     1141 agctcggcgc gacgcgcgcg gcgtccgccg cggaggccgc caggcaggct gagatggtcg
     1201 tcaccatgct gccgtcgtca ccgcacgtcg aggccgtgta cctgggcgcc ggcgggatcc
     1261 tcgagggcgt ggcgcggggc cggctgtgcg tggacatgtc cacgatcgag ccggcggtgt
     1321 cgcggcgcgt ggccgaggtg gcgcggcagc gaggcgtgcg cttcgtggac gcgccggtgt
     1381 cgggcggcgt cccgcgcgcc gaggacggca cgctcgcgat catggtgggc ggcgaggcgc
     1441 gcgacgtgga ggaggcgcgc ccgatcctcg cggcgatggg ctccaccgtc atccacgtcg
     1501 gcccggtcgg cgccggcgag gtcgccaagc tctgcaacaa cctgatcgcc ggggtggcgg
     1561 ccgtcgccgt cagcgaggcc ttccggatcg ccgagggctt cggggtggac ccgaagatcc
     1621 tgaccgaggt catcgcgaag tcctcgggtc acacgtggct catggagcac atgcacccgg
     1681 tgcccgggct cgtcccgcgc tccgcgtcga gccgcgacta cgcgcccggc ttcatgaccg
     1741 acctcatggc caaggacttg ggcctggccg tcaacgcggc ccgcaccctg cgcgtgccgg
     1801 tgttcgtggc gccggcggcg cagcaggtgc tgcgcctggc gtcctcgcac gggctcggcc
     1861 gcaaggactt cacgtccgtc tatcaattcc tgcggccctc cgacggcgcc gccccggtgt
     1921 gacgatgcta tactgtcgcc cgttcatgcc gaggaggccc cagcggtgag tctctacatc
     1981 atgctcagca cgctgtcgga gtccggccgc aaggtgctgc gcggccggcc gggatggatc
     2041 cgcaaggtca acgccgacgt gcagcgcatg ggcgcgcgcg tggtcgcgca gtacgccgtc
     2101 ctcggcccct acgacttcgt caccgtcatc gaggcgccgg acaacgccac gatctcgcgc
     2161 gtgtcggtgg agctgggcgc acgcggcggc gtcgccggca tgaccatggc cgccatcccg
     2221 ctcgaagagt tcatcactcg cctcgagcgc agcggcaaca agaggagcac gcggaagaag
     2281 cgatgagagt cctcaagctc atacccgagc gctgcacggg ctgtctccgc tgcgagctgg
     2341 cgtgctcgta catgcagacg ggggagttca agcccgcgaa gtccgtcatc cgggtgtcgc
     2401 ccttcgaggg ccacacctcg tacgccccgt acacctgcac gcagtgcgcc gagggctggt
     2461 gcatgacggc gtgtcccgtc ggggccatcg ttatcagcgc ggtgggcgcc aaggacgtgg
     2521 tggacgaccg ctgcgtcggc tgcaagctgt gcacgatcgc ctgtccgtac gggacgatgt
     2581 tctacgaccc cgacacccgc aaggccttca agtgcaatct ctgcggcggc gatcccgcgt
     2641 gcgcccacgc gtgtcccacc gccgccatcc tctacgagga gacggccacc gtggactggc
     2701 tgggtgagtt cgcggccgcg cgcaccagcc ggctgctggc gggagtgtcc tgatgccggc
     2761 gcgtcatctg atcatcggcg gcggcaccgc ggggatgaac gcgatccgca cgatccgcga
     2821 ggaggagcgc gagcgctcgg agatcacgct cgtcaccggc gagaagccgt actcccggat
     2881 ggtg
//
LOCUS       DATIAZ010000116         6060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_555386, whole genome shotgun sequence.
ACCESSION   DATIAZ010000116 DATIAZ010000000
VERSION     DATIAZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555386"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VLF19_03985"
     CDS             complement(<1..653)
                     /locus_tag="VLF19_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSE92443.1"
                     /translation="MSAAPRKDPAAVEAEVREKIHLEILKRTGAYHANDHVLLPSGQH
                     TSEYIEKTLVTTEPSFTEGLGAVIAKHFAPWPIDVVLSTGPGALILSHCVARAHPSRP
                     IVVYGTKGLAGGKRRVTLPVEFQRLIREGTRVLLIEDLVSTGSTLNLLIGLVEQLGAE
                     VVGIGCLWRRTSVDLNGKPVFSLVSRDFPTYNPSECPLCRKGIALNEEFVRRRNQRRP
                     "
     gene            721..1395
                     /gene="tsaB"
                     /locus_tag="VLF19_03990"
     CDS             721..1395
                     /gene="tsaB"
                     /locus_tag="VLF19_03990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSE92444.1"
                     /translation="MRLLAVETSTLAGGVALLDGDRLRGEYLLDVTVTHSERLMPAID
                     RLLGDAGWPPESLEALAVAVGPGSFTGLRIGLAAVKGLALALGLPIAAVPTLDAMAET
                     LPFAAMPVCPVLDARKGEVYSCLYRWEGAGMRRDWDYVALAPDALAARLTEPAILLGD
                     GASLIRSPHARLAPPHRRGPSPAAVGVLGRARLAAGQTARAAELVPIYLRPSEAELKR
                     GGLPRH"
     gene            1400..1870
                     /gene="rimI"
                     /locus_tag="VLF19_03995"
     CDS             1400..1870
                     /gene="rimI"
                     /locus_tag="VLF19_03995"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSE92445.1"
                     /translation="MRASDLDEVLSIERASFTMPWSRGAFLYEMEQNQVARCFVIREE
                     GRLVGYLCLWEVADELHITNIAVRPDARRRGIGRTLLGRTLDDARNRALRLVVLEVRP
                     SNTEARALYESFGFRVVGRRRGYYYDTGEDALVMEASLGRGGSREAPGGNRRYR"
     gene            2014..2796
                     /locus_tag="VLF19_04000"
     CDS             2014..2796
                     /locus_tag="VLF19_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSE92446.1"
                     /translation="MFGATGGCGCHTVPKGPVNAGGRRYDGPFGTVYSTNVTPDRQTG
                     IGDWTDEQIATAVRLGRRPNGERLLPVHPFTVFNGMAAEDLKALGAFLRTVPAVNRAN
                     QPKRITVPLFESVFLPAWLVAFAPRETPPPSAPTSGLARGEYLVRAVAHCGECHTPRT
                     VTMATDNSRFLAGNAKGPEDSQVPNITPHKETGLTWSEEEIAEYLATGNRPDGDVAGG
                     LMGEVIQGTLAGYKDMTRSDLLAIARYLKSIPAVRNKVVRQP"
     gene            2933..3322
                     /locus_tag="VLF19_04005"
     CDS             2933..3322
                     /locus_tag="VLF19_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSE92447.1"
                     /translation="MSTPDAIAARQQLMKDQGAASRSIQEKLKTGQVQAVASDAEKLA
                     ETAMKIPALFPQGSLDPKTSRAKPEIWQKRSEFEGYAKTLNTKATQLAATARTGNADS
                     VKAAVADLGKTTCTACHNAFRGPEIKK"
     gene            3386..4384
                     /gene="meaB"
                     /locus_tag="VLF19_04010"
     CDS             3386..4384
                     /gene="meaB"
                     /locus_tag="VLF19_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSE92448.1"
                     /translation="MAGAGAPSRKNPATLVERMLAGDRLALARLITHVESRTEAVPAI
                     MRAVHARTRHAYVVGVTGPPGAGKSTVVDRLIAALRPAGHTVGVVAVDPSSPFTGGAV
                     LGDRIRMQAHALDPGVFIRSMATRGAFGGLARATGNVVKLLSAFGLDRILIETVGVGQ
                     TELDIMKLADTTVVVLVPESGDAIQTMKAGLMEAADVFVVNKADRGGAHALMAELKFA
                     AHLAHTSPAARRDVDWEVPVLATEAQTGVGIDELLATIERHRETLEAAGALAERRRAR
                     RREEFRSLLVEQLTTTIERRLTSGDLAGTFEDVADGRLDPYSAAEAVLGVLSLKTP"
     gene            4381..>6060
                     /locus_tag="VLF19_04015"
     CDS             4381..>6060
                     /locus_tag="VLF19_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSE92449.1"
                     /translation="MTPSVPFRDTFWNVPVWAQIALYVGGAVALAILGYGTWQRVRLW
                     RQGKPEHRRDRIPERVKLVAMHALGQVRTLSQAYPGVMHAIMFWGFLALFMGTVLATI
                     DYDVTLPLFGYKLLKGSFYLLYETVLDLFGLFFVIGLGMAVWRRFVLRPHRIDPTARF
                     AGVLALLFVINVTGFVMEAARLAVVTPEWGPWSPVGWALAQAMLGAGMSEGALRATHL
                     GVWLFHAAISLGFIAIIPYSYFVHFFTTPLNIFFAKLTPRGEITKIENIEEAEALGVS
                     RLEEFSWKRRLDFDACVECGRCQAACPAYLAGTALSPKRIIVKLKRHMHGDLPGPIHG
                     ELIKPEELWACTTCMACVQECPAFIDIVDTIIDLRRYLALSEGALPSTAPQSLQNIQR
                     AGNPWGLPAGERLAWAEGLDVPVMEPGKEVEYLYWVGCSASYDKRNQAIARAVVKILK
                     TAGVSFAVMAEERCHGEVARRLGEEYLYQTVQQENVEAINQYRFGKVLAHCPHCFNTI
                     KNEFPQLGGTYEVVHHSVLIQELLASGRLRPRRAIDATIAFHDSCYLGRYNG"
BASE COUNT          892 a         2151 c         2089 g          928 t
ORIGIN      
        1 gggcgccgct ggttacggcg tctgacgaac tcctcattca gcgctattcc cttcctgcaa
       61 agtggacact ctgatgggtt gtatgtcgga aagtcacgac tgacgaggct gaagaccggc
      121 tttccgttca ggtccacgga cgtccgtctc cacagacacc ctatcccgac gacctcggct
      181 ccgagctgct ccaccaggcc gatgaggagg ttgagggtcg agcccgtgct cacgaggtcc
      241 tcgatgagga gcacccgcgt accctcgcgg atcagacgct ggaactcgac gggaagggtc
      301 acccgacgct tgccgccggc aagtcccttc gtgccgtaca cgacgatggg ccgcgagggg
      361 tgggcccgcg ccacacaatg ggaaaggatc agcgcgcccg gacccgtgga gaggacgacg
      421 tcgatcggcc acggggcgaa gtgcttggcg atgaccgccc cgagcccctc ggtgaacgag
      481 ggctccgtcg tcaccagcgt cttctcgatg tactcgctcg tgtgctgacc ggatggcagc
      541 agcacgtggt cgttggcgtg gtaggcgccg gtgcgcttga ggatctcgag gtggatcttc
      601 tcgcggacct ccgcctcgac ggcggcggga tccttccttg gggcggcgct catacgaccc
      661 tcctggagat attatcgcat gcgcccgacg aggtgtcgga ccggtgttag acttgccggc
      721 gtgcgcctcc tcgcggtcga gacctcgacg ctggccggag gcgttgcgct cctcgacggc
      781 gaccggctcc gcggtgagta tctgctcgac gtgaccgtca cgcactcgga acgtctgatg
      841 ccggcgatcg accggctgct cggcgacgcg ggctggccgc cggaaagcct ggaggccctc
      901 gccgtggccg tgggccccgg ctcgttcacg ggtctgcgca tcgggctcgc cgccgtcaag
      961 ggcctggcgc tggccctcgg tctgcccatc gccgccgtgc cgacgctcga cgcgatggcg
     1021 gagacgcttc cgttcgcggc gatgcccgtg tgtcccgtgc tcgacgcgcg caagggcgag
     1081 gtgtacagct gtctttaccg atgggagggc gcgggcatgc gccgagactg ggactacgtc
     1141 gcgctcgctc ccgatgcgct ggccgcgcgg ctcacggagc cggcgatcct gctcggcgac
     1201 ggcgcgtcgc tgatccgctc gccgcacgcg cggctggcac cgccgcaccg tcgcgggccg
     1261 tcacccgcgg cagtgggcgt gctgggccgc gcacggctcg ccgcgggaca gaccgcgcgg
     1321 gccgcggaac tggtgccgat ctatctccgg ccgtcggagg cggagctcaa gcgcggtggt
     1381 ctccctcgcc attgagcgga tgcgcgcgtc ggacctcgac gaggtgctct cgatcgagcg
     1441 cgcctcgttc accatgccat ggtcacgcgg cgccttcctc tacgagatgg agcagaacca
     1501 ggtcgcccgc tgcttcgtga tccgtgagga aggccggctc gtcggttacc tctgcctctg
     1561 ggaggtcgcg gacgagctgc acatcacgaa catcgccgtg cgtcccgacg cccggcggcg
     1621 cggcatcggc cgcacgttgc tcggccgcac gctcgacgac gcccggaacc gcgcgctccg
     1681 gctcgtagtc ctggaggtcc ggccgagcaa caccgaggcg cgggcgctgt acgagtcgtt
     1741 cggcttccgg gtggtcggcc gccgccgggg ctactactac gacaccgggg aggacgccct
     1801 ggtcatggag gcctcgctcg gtcggggtgg cagccgggag gcgccgggcg ggaaccggcg
     1861 ctaccgctga caggttattc gggtcgaccg ccgatgggtc tcctatcgca cgtgaggtgc
     1921 gccactatcg taggcgctct gtcgctcccg atcctgacac cgctctctgc ggccggacag
     1981 gcctcgtccg ccgagatcga gcggggccgt tacgtgttcg gcgccaccgg cggctgcggt
     2041 tgccacacgg tgcccaaggg cccggtgaac gccggcggtc gccgctacga cggcccgttc
     2101 ggcacggtct actcgaccaa cgtcacgccc gaccggcaga ccggcatcgg cgactggacc
     2161 gacgagcaga tcgccaccgc cgtccgcctc ggccgacggc cgaacggcga gcgcctgctg
     2221 ccggtgcacc cgttcaccgt gttcaacggc atggcggccg aggacctcaa ggcgctcggc
     2281 gcgttcctgc gcacggtgcc ggccgtcaat cgcgccaacc agcccaagcg catcacggtg
     2341 ccgctcttcg agtcggtctt cctgcccgcc tggctcgtgg ccttcgctcc gcgcgagacc
     2401 ccgccaccgt cggcgccgac atccgggctg gcgcgtggcg agtacctcgt gcgtgcggtc
     2461 gcccactgcg gcgagtgcca cacgccgcgc acggtgacca tggcgaccga caactcccga
     2521 ttcctcgcag gcaacgccaa gggtcccgag gactcgcaag tccccaacat caccccgcac
     2581 aaggagacgg ggctgacgtg gtcggaggag gagatcgccg agtacctcgc caccggcaac
     2641 aggccggacg gtgacgtggc cggcggcttg atgggcgagg tgatccaggg aacgctggcc
     2701 ggctacaagg acatgacgcg gtccgacctc ctggcgatcg cccggtacct caagtcgatt
     2761 ccggcggtga ggaacaaggt cgtcaggcag ccgtagcgaa ccatcaccca tgggcgccgg
     2821 ccaccggcca ccgcccgcaa caggaggtca cgtcatgaga ttgtcgatgc gttcgggcgt
     2881 cctcgtcgcg agtgccacgg ccgtcctcgg ttgtgccacc ggaggagcca tgatgagcac
     2941 cccggatgcg atcgcggccc gccagcagct catgaaggac cagggcgcgg cctcgcggtc
     3001 gatccaggag aagctcaaga ccgggcaggt ccaggccgtc gcctcggacg cggagaagct
     3061 cgcggagaca gccatgaaga tcccggccct gttcccgcag ggctcgctcg atcccaagac
     3121 ctcgcgcgcg aagccggaga tctggcagaa gcggtcggag ttcgaggggt atgcgaagac
     3181 gctcaacacg aaggcgaccc agcttgccgc gaccgcgcgc acgggcaacg ccgactcggt
     3241 caaggccgcg gtcgcagacc tcggcaagac cacctgtacg gcctgtcaca acgcgttccg
     3301 cggccccgag atcaagaagt agctcgccga ctccggggag ggcgggcggc ccgccctccc
     3361 cgtgctagaa tccggccgct ttccgatggc cggcgccggc gccccctccc gcaagaaccc
     3421 cgcgacgctc gtggagcgca tgctggccgg cgaccggctc gcgctcgccc ggctcatcac
     3481 gcacgtggag agccggaccg aggcggtgcc cgcgatcatg cgcgcggtgc acgcgcgcac
     3541 ccggcacgcc tacgtggtcg gtgtcaccgg tccgccgggc gccggcaagt ccaccgtggt
     3601 ggaccgcctc atcgccgcgc tccggccggc cggccacacc gtcggcgtcg tcgccgtgga
     3661 tccgtcgagc ccgttcacgg gcggcgcagt gctcggtgac cgtatccgca tgcaggcgca
     3721 cgcgctcgac cccggcgtgt tcatccgtag catggccacg cgcggggcgt tcggcggcct
     3781 ggcacgcgcc accggcaacg tcgtcaagct gctgtcggcc ttcgggctgg atcggatcct
     3841 catcgagacc gtcggggtgg gccagacaga gctcgacatc atgaagctcg ccgacaccac
     3901 ggtggtcgtg ctcgtcccgg agtctggcga cgcgatccag accatgaagg ccgggctcat
     3961 ggaggccgcc gacgtcttcg tcgtcaacaa ggccgaccgc ggcggcgcac atgccctgat
     4021 ggcggagctg aagttcgccg cgcacctggc gcacacgagc cccgccgcgc gccgcgacgt
     4081 cgactgggag gtccccgtcc tggccacgga ggctcagacc ggggtgggca tcgacgagct
     4141 gctcgcgacg atcgagcgcc accgcgagac gctcgaggcc gccggcgcgc tcgccgagcg
     4201 gcggcgcgcc cgccgccggg aggagttccg gagcctgctc gtggagcagc tcaccaccac
     4261 gatcgagcgg cggctgacgt ccggcgacct cgccggcacg ttcgaggacg tcgccgacgg
     4321 ccggctcgac ccctactcgg cggccgaggc cgtgctcggc gtgctatcgt tgaaaactcc
     4381 atgacgccga gcgtcccgtt ccgcgacacc ttctggaacg tcccggtctg ggcgcagatc
     4441 gcgctctacg tcggcggcgc cgtcgcgctc gcgatcctcg ggtacgggac gtggcagcgc
     4501 gtgcggctgt ggcggcaagg caagcccgag caccgccgcg atcgtatccc ggagcgcgtg
     4561 aagctcgtcg ccatgcacgc gctcggccag gtccggacgc tctcgcaggc gtatccgggg
     4621 gtcatgcacg cgatcatgtt ctggggattc ctggccctgt tcatgggcac ggtgctggcg
     4681 accatcgact acgacgtcac gctgcccctg ttcggctaca agctcctcaa gggctccttc
     4741 tatctcctct acgaaacggt gctcgacctc ttcgggctgt tcttcgtgat cggcctcggc
     4801 atggcggtgt ggcggcgctt cgtgctgcgc ccgcaccgca tcgatcccac ggcccgattc
     4861 gccggggtcc tcgccctgct cttcgtcatc aacgtcaccg ggttcgtgat ggaggcggcg
     4921 cggctcgccg tggtcacgcc cgagtggggg ccgtggtcgc cggtcggctg ggcactggcg
     4981 caggcgatgc tcggggcagg gatgtcggaa ggggcgctcc gcgccacgca cctcggcgtc
     5041 tggctcttcc acgcggcgat ctcgctcggg ttcatcgcga tcatcccata ctcctacttc
     5101 gtgcacttct tcaccacacc gctcaacatc ttcttcgcca agctcacgcc gcgcggcgag
     5161 atcacgaaga tcgagaacat cgaggaggcc gaggcgctcg gcgtctccag gctggaagag
     5221 ttctcgtgga agcggcgcct ggacttcgac gcctgcgtgg agtgcgggcg ctgccaggcc
     5281 gcgtgccccg cctacctggc gggcaccgcg ttgagtccca agcggatcat cgtgaagctc
     5341 aagcgtcaca tgcacggcga tctgcccggg ccgatccacg gcgagctgat caagcccgag
     5401 gagctgtggg cctgcacgac ctgcatggcc tgcgtgcagg agtgccccgc gttcatcgac
     5461 atcgtggaca cgatcatcga cctccgccgc tacctggcgc tgtcggaggg cgcgctgccg
     5521 tcgacggcgc ctcagtcgct ccagaacatc cagcgcgccg gcaacccgtg ggggcttccg
     5581 gcgggggagc gcctggcgtg ggccgagggg ctcgacgtgc cggtgatgga gccgggcaag
     5641 gaggtcgagt acctctactg ggtcggctgc tccgcgtcct acgacaagcg caaccaggcc
     5701 atcgcgcggg cggtggtcaa gatcctcaag accgccggcg tctcgttcgc ggtcatggcc
     5761 gaggagcgct gccacggcga ggtagcgcgc cggctgggcg aggagtatct ctaccagacc
     5821 gttcagcagg agaacgtcga ggccatcaac cagtaccggt tcgggaaggt gctggcgcac
     5881 tgcccgcact gcttcaacac catcaagaac gagttcccgc agctcggcgg cacctacgag
     5941 gtcgtccacc actcggtgct gatccaggag ctgctcgcct ccggccgcct ccggccccgg
     6001 cgggcgattg acgcgaccat cgccttccac gactcctgct acctcggccg ctacaacggc
//
LOCUS       DATIAZ010000117         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_423498, whole genome shotgun sequence.
ACCESSION   DATIAZ010000117 DATIAZ010000000
VERSION     DATIAZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423498"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VLF19_04020"
     CDS             complement(<1..240)
                     /locus_tag="VLF19_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSE92450.1"
                     /translation="MSQGRIVQVSVSPGGVPKRPVEAALVTALGVEGDAHRDTEHHGG
                     PERAVCLYALEAIEALQHEGHPIVPGALGENLTVAG"
     gene            complement(321..800)
                     /locus_tag="VLF19_04025"
     CDS             complement(321..800)
                     /locus_tag="VLF19_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HSE92451.1"
                     /translation="MIGHGLTWEEQSVGAVYRTLARTISETDIVNFVNLCGFTEPLFM
                     DMEYVARESVFGRRAAPGALTFALAEGLIMQTGLIHGTGMAWLGGEIRIAAPVLAGDT
                     INVEIEVADKRVTKRPDRGIVTYQHRIRNQRGDLVMEVRVQRMIKRRERAPGAAEGR"
     gene            complement(826..1527)
                     /locus_tag="VLF19_04030"
     CDS             complement(826..1527)
                     /locus_tag="VLF19_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92452.1"
                     /translation="MLTVDDLHVYYGEIHALKGVALEVHRGEIVAILGNNGAGKTTTL
                     KTISGLLVPRRGTVTLDGAPLTGVPAHEVVLRGIAHVPEGRRIFNRLTVRENLMMGAY
                     LRKDGGIDADLDRVFALFPRLKERVAQVAGTLSGGEQQMLAIARALMASPRVLLLDEP
                     SMGLAPVLVEQIFETILDINRQGTTILLVEQNAAMALAHAHRGYVLETGTVAFAGTAA
                     ALADAPDVRRAYLGE"
     gene            complement(1511..2311)
                     /locus_tag="VLF19_04035"
     CDS             complement(1511..2311)
                     /locus_tag="VLF19_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92453.1"
                     /translation="MTALFEARSVTKRFGGLTAVSQVDFALASGIASIIGPNGAGKTT
                     LFNVFTGLYTPDEGSLTFKGHSLVGLRPDQITALGMCRTFQNIRLFANMTAVENVLVG
                     MHARVRLGLWDVLARGPRFVGTESRLWAHATELLDRVGLRPAGNEIARNLPYGDQRRL
                     EIARALASEPTLLLLDEPTAGMTQGEAATLMDLLRRLVGDLGLAILLIEHNMRVVMGV
                     SDRVTVLDHGEKIAEGPPQDVQADPRVVEAYLGRGFTDRAGSQRADGR"
     gene            complement(2308..>2568)
                     /locus_tag="VLF19_04040"
     CDS             complement(2308..>2568)
                     /locus_tag="VLF19_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92454.1"
                     /translation="TVVTVLNLQVLKGLSLWLNELKNAGVTILGYNLADLPTQLEPAK
                     YERMIFGMILVLMMIFRPQGILPARRRQRELAGTRREATAAP"
BASE COUNT          362 a          902 c          919 g          385 t
ORIGIN      
        1 gcccgcgacg gtgaggttct cgccgagcgc ccccgggacg atcgggtgac cctcgtgctg
       61 gagcgcctcg atggcctcca gcgcgtagag gcacacggcc cgctcgggac cgccgtgatg
      121 ctcggtgtca cggtgggcgt cgccctccac gccgagcgcc gtcacaagcg ccgcctcgac
      181 gggccgcttg ggcacgcccc ctggcgacac gctcacctgc acgatgcgtc cctggctcac
      241 cgacccaccc ccgggccgat gccctcggcc ctcgcgcgat tgtacgcgag gccccggggg
      301 cgcgaggtcc tcgagtcccg tcagcggccc tcggcggccc ctggcgcgcg ctcgcgtcgc
      361 ttgatcatgc gctgcacgcg cacctccatc accaggtcgc cgcgctggtt ccggatgcgg
      421 tgctggtagg tgacgatgcc gcggtcgggt ctcttcgtca cgcgcttgtc ggccacctcg
      481 atctcgacgt tgatcgtgtc gccggcgagc acgggggcgg cgatgcggat ctcgccgccg
      541 agccacgcca tgccggtgcc gtggatcagc cccgtctgca tgatcaggcc ctcggcgagc
      601 gcgaaggtga gcgcgcccgg cgcggcgcgc cgcccgaaga cggactcgcg cgccacgtat
      661 tccatgtcca tgaagagggg ctcggtgaag ccgcacaggt tgacgaagtt cacgatgtcc
      721 gtctcggaga tcgtgcgggc gagcgtccgg tagacggccc cgacgctctg ctcctcccag
      781 gtgagtccgt ggccgatcat cggacgcctc cctgcatgcg cgcgctcact ccccgagata
      841 ggcgcgcctg acgtcggggg cgtcggcgag cgcggccgcc gtgcccgcga aggccacggt
      901 accggtctcc aggacgtagc cgcgatgggc gtgggccagg gccatggcgg cgttctgctc
      961 gaccagcagg atcgtcgtgc cctggcggtt gatgtcgagg atggtctcga agatctgctc
     1021 gaccaggacc ggcgcgagcc ccatgctcgg ctcgtcgagc aggagcacgc gcgggctcgc
     1081 catgagcgcg cgggcaatgg cgagcatctg ctgctcgccg ccggagagcg tgcccgcgac
     1141 ctgggccacg cgctccttga gccgcggaaa gagcgcaaag acgcggtcga ggtcggcatc
     1201 gatgcctccg tctttccgga ggtaggcgcc catcatgaga ttctcgcgga ccgtcagccg
     1261 attgaagatc cgccgcccct cggggacgtg ggcgatgccg cgcagcacca cctcgtgggc
     1321 gggcacgccg gtcagggggg cgccgtcgag ggtcaccgtg ccccgccgcg gcaccaggag
     1381 gcccgagatc gtcttcagcg tggtggtctt gccggcgccg ttgttgccga ggatggccac
     1441 gatctccccc cggtgcacct ccagcgccac gcccttgagc gcgtggatct cgccgtagta
     1501 gacgtgcagg tcatcgaccg tcagcacgct ggctccccgc gcggtccgtg aagccgcgcc
     1561 cgaggtaggc ctcgaccacg cgcgggtcgg cctgcacgtc ctgtggcggg ccctcggcga
     1621 tcttctcgcc gtggtccagc acggtgacgc ggtcggagac gcccatcacc acccgcatgt
     1681 tgtgctcgat cagcaagatg gccagcccca ggtcgccgac gagccggcgc agcaggtcca
     1741 tgagggtcgc cgcctccccc tgcgtcatgc ccgccgtcgg ctcgtccagc agcagcagcg
     1801 tcggctcgga tgccagggcg cgcgcgatct cgagccgccg ctggtcgccg tacggcagat
     1861 tccgcgcgat ctcgttgccg gccgggcgga gacccacccg gtcgagcagc tccgtggcat
     1921 gggcccacag gcgcgattcg gtgccgacga agcggggtcc ccgcgcgagc acgtcccaca
     1981 gccccaggcg gacccgcgcg tgcatgccaa ccagcacgtt ctcgaccgcc gtcatgttgg
     2041 cgaagagccg gatgttctgg aacgtccggc acatgcccag cgccgtgatc tggtcgggcc
     2101 ggaggcccac gagcgagtgg cccttgaagg tcagcgagcc ctcgtccgga gtgtagaggc
     2161 cggtgaagac gttgaagagc gtcgtcttgc cggcgccgtt gggaccgatg atcgaggcga
     2221 tgcccgaggc gagggcgaag tcgacctggc tgacggcggt gaggccgccg aagcgcttgg
     2281 tgacggaacg agcttcgaag agcgcggtca cggcgccgcc gtggcctcgc gcctggtccc
     2341 ggccagctcg cgctgccgac ggcgcgcggg gaggataccc tgaggccgga agatcatcat
     2401 gaggacgagg atcatgccga agatcatccg ctcgtacttc gccggctcca gctgggtggg
     2461 gaggtcggcc aggttgtagc cgaggatggt gacgccggcg ttcttgagct cgttgagcca
     2521 cagcgacagg cccttgagga cctgaaggtt gagcacggtc acgaccgt
//
LOCUS       DATIAZ010000118         3107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1607431, whole genome shotgun sequence.
ACCESSION   DATIAZ010000118 DATIAZ010000000
VERSION     DATIAZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607431"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..2528)
                     /gene="ctaD"
                     /locus_tag="VLF19_04045"
     CDS             complement(51..2528)
                     /gene="ctaD"
                     /locus_tag="VLF19_04045"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HSE92455.1"
                     /translation="MATRHLFVDDHASAAQHAAAQTGLWSWITTVDHKRVGILYGVSG
                     FVFFLVGGVEALVIRLQLARPESAVVSAGAFNALFTMHGTTMVFLAVMPFNAALFNYL
                     VPLMIGARDVAFPRLNALSFWIFLAGALFLNASFLVGAPPDAGWFGYANLTSRRFSPG
                     LGVDFWALSLQVLGVSSLMAAVNFIVTILNLRAPGMTFMRMPVFVWMTLVVQFLILLA
                     FPPITVGLIFLTFDRFFGTNFYEVAAGGDLHLWQHLFWIFGHPEVYILILPAFGIVSE
                     VLPTFARKPLFGAPVVIYSGVLIGFFGFGVWSHHMFAAGMGPIADAAFAIATMLIAIP
                     TGVKIFNWLATLWGGSIRGTTAMHFAVGLIAMFTIGGLSGIMHASPPVDLQQTDTYFV
                     VAHFHYVLIGGSLFGLFAGAYYWWPKITGRFLGEGLGRLNFWTLFAAFNLAFFPQHYL
                     GAIGMPRRIYTYRAGVGWDFWNLASTVGAFGIGFAVLLFAVNAVRSLRAGARAEADPW
                     DGRTLEWRTTSPPPPHNFDEIPPVHGRDTFWREKHPSARRGGAGPTTPPPPSHGASSR
                     PEATVLAGATPDHADVHLPAPSHWPIVLALGLGVAVAGALIHVVVVVLGGALMLYAAF
                     RFALEHHRNPVHVHQEGTLGIDHRKVAVWTFIGSECLFFGTLIATYMAYRGRSVVGPY
                     PADILNLPLTTLSTFDLLMSSLLMVLALAAVQRGDHAQTRLWLAGTAFFGLIFLGFQA
                     YEFTHFVHDGLTLQRNLFGSTFFVLTGLHGAHVTLGVLWLLSLLVLELRGRLGVTDAV
                     KVEVAGLYWHFVDVVWIVIFTLVYLIP"
     gene            complement(2536..>3107)
                     /locus_tag="VLF19_04050"
     CDS             complement(2536..>3107)
                     /locus_tag="VLF19_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSE92456.1"
                     /translation="VTANELYLPLGRPVHLVLEGPDVIHSFWVPQLGGKRDLVPGRIN
                     HLSLTPEAVGEYLGQCAEFCGVSHANMGMRVIVVPPEEFDRWVAAQRAPAAEPGGPAA
                     EGKAVYTALACVGCHTIKGVSAGVLGPDLTHFGSRRTVAGGLLPNTPDTLAAWIRDPQ
                     SVKPGAKMPSLGLSAAQARAVAAYLLSLK"
BASE COUNT          573 a          988 c         1163 g          383 t
ORIGIN      
        1 gatgaccgcg accttgacgt acgtcccgac gttcgcgtgc ccgggctcgc tcatgggatg
       61 aggtagacga gggtgaagat cacgatccac acgacgtcga cgaagtgcca gtagagcccg
      121 gccacctcga ccttcacggc gtcggtgaca ccgaggcggc cgcgcagctc gaggacgagc
      181 agcgagagca gccacagcac gccgagcgtc acgtgggctc cgtggaggcc cgtcaggacg
      241 aagaaggtgg agccgaagag gttccgctgg agcgtgagac cgtcgtggac gaagtgcgtg
      301 aactcgtacg cctggaagcc gaggaagatg aggccgaaga aggccgtgcc ggcgagccag
      361 agccgcgtct gcgcgtggtc gccgcgctgc acggcggcga gggccagcac catgaggagg
      421 ctcgacatca ggaggtcgaa cgtgctcagc gtcgtgagcg ggaggttcag gatgtcggcg
      481 gggtagggac cgacgacact gcggccacgg tacgccatgt aggtggcgat gagcgtgccg
      541 aagaagaggc attcggagcc gatgaaggtc cacacggcga ccttgcggtg gtcgatgccg
      601 agcgtcccct cctggtggac gtgcacggga ttgcggtggt gctcgagcgc gaagcggaac
      661 gcggcgtaga gcatcagcgc cccgccgagg acgacgacga cgacgtggat cagggccccg
      721 gcgacggcca cgccgagccc cagcgcgagc acgatcggcc agtgcgacgg cgcgggcagg
      781 tggacatccg cgtggtcagg ggtggccccg gcgagcacgg tcgcctccgg gcggctcgag
      841 gcgccgtgac tcggcggcgg cggcgtggtc gggccggcgc cgccgcgccg cgcgctcggg
      901 tgcttttcgc gccagaacgt gtcgcggccg tggacgggtg gaatctcgtc gaagttgtgg
      961 ggtgggggcg gcgacgtcgt gcgccactcg agcgtgcggc cgtcccaggg atcggcctcg
     1021 gcccgggccc cggcgcggag gctccgcacc gcgttgaccg cgaacagcag gacggcgaac
     1081 ccgatgccaa aggcgcccac cgtggaggcc aggttccaga agtcccagcc gacgcccgcg
     1141 cggtacgtgt agatgcgacg gggcatgccg atcgcgccga ggtagtgctg ggggaagaag
     1201 gccaggttga aggcggcgaa gagcgtccag aagttgaggc gcccgaggcc ctcgccgagg
     1261 aagcgcccgg tgatcttcgg ccaccagtag tacgcgccgg cgaacagccc gaagaggctg
     1321 ccgccgatca acacgtagtg gaagtgcgcg acgacgaagt acgtgtccgt ctgctgaagg
     1381 tcgactggcg gcgaggcgtg catgatgccg ctgagcccgc cgatggtgaa catcgcgatc
     1441 agtccgacgg cgaaatgcat ggccgtcgtg ccccggatcg agccgcccca gagggtggcc
     1501 agccagttga agatcttcac gccggtcgga atggcgatga gcatggtggc gatcgcgaag
     1561 gcggcatcgg cgatgggccc catgccggcg gcgaacatgt ggtggctcca cacgccgaag
     1621 ccgaagaacc cgatgagcac gcccgagtag atgaccaccg gtgcgccgaa gagcggcttg
     1681 cgcgcgaagg tcggcagcac ctcggacacg atgccgaacg cgggcaggat caggatgtag
     1741 acctcggggt ggccgaagat ccagaagagg tgctgccaca ggtggaggtc gccgccggca
     1801 gcgacctcgt agaagttcgt gccgaagaac cggtcgaagg tgaggaagat cagcccgacc
     1861 gtgatcgggg ggaacgcgag caggatgagg aattgcacga ccagcgtcat ccacacgaac
     1921 acgggcatgc gcatgaacgt catgcccggt gcgcgcaggt tgaggatcgt cacgatgaag
     1981 ttcaccgccg ccatcagcga cgagacgccg aggacctgga ggctcaacgc ccagaagtcg
     2041 acgccgagcc ccggcgagaa ccggcgcgac gtgaggttgg cgtagccgaa ccagccggcg
     2101 tccggcggcg caccgacgag gaacgacgcg ttcagaaaca gcgcgccggc caggaagatc
     2161 cagaaggaca gcgcgttcag ccgcgggaag gcgacgtcgc gggcgccgat catgagcggc
     2221 accaggtagt tgaagagggc ggcattgaag ggcatgacgg ccagaaacac catcgtggtg
     2281 ccgtgcatgg tgaagagcgc gttgaacgcc ccggcgctca ccaccgcgct ctccgggcgc
     2341 gcgagctgca ggcggatcac cagggcctcg acgccgccca ccaggaagaa gacgaagccg
     2401 ctgacgccat agaggatgcc gacgcgcttg tggtcgaccg tcgtgatcca cgaccagaga
     2461 ccggtctgcg cggccgcgtg ctgtgccgcg gacgcgtgat cgtcgacgaa caggtggcgc
     2521 gtcgccatca cggcctcact tcaggctcag caggtacgcc gccaccgccc gggcctgggc
     2581 cgcgctgagg ccgaggctcg gcatcttcgc ccccggcttc accgactgcg ggtcgcggat
     2641 ccacgcggcg agggtgtcgg gcgtgttggg caacaagccg ccggcgacgg tccggcggct
     2701 gccgaagtgc gtgaggtcgg gtccgagcac gccggccgag acccccttga tcgtgtggca
     2761 gccgacacac gcgagggcgg tgtagaccgc cttgccctcg gcagcgggcc ccccgggctc
     2821 cgccgccggc gcgcgctgcg ccgcgaccca gcgatcgaac tcctcggggg gcaccacgat
     2881 cacgcgcatg cccatgttgg cgtgcgatac gccgcaaaac tccgcgcact gcccgaggta
     2941 ctcacccacg gcctccggcg tcagcgagag atggttgatc cgccccggca cgagatcgcg
     3001 cttgcccccg agctggggca cccagaacga gtggatgacg tcgggccctt cgaggacgag
     3061 gtggacgggc cgccccaggg gcaggtacag ctcgttcgcc gtcacca
//
LOCUS       DATIAZ010000119         5084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_424116, whole genome shotgun sequence.
ACCESSION   DATIAZ010000119 DATIAZ010000000
VERSION     DATIAZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424116"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VLF19_04055"
     CDS             <1..880
                     /locus_tag="VLF19_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSE92457.1"
                     /translation="ARAQLALARAQLTAARSQLAEIQAQLEQAERDFARQQELLRREL
                     VAAQSMETARAQRDTLRARLAAAEEQIKVAQQSVSVAQVQLDNTEIRAPFTGMVVAKS
                     AQPGEMISPISAGGGFTRTGIGTIVDMDSLEIQVDVNEAYINRVTPGQPVQATLNAYP
                     DWKIPGSVIAIIPTADRSKATVKVRIAIKQRDARIVPDMGARVAFLEKEPARRASPAE
                     AGVLVPAEAVRGDGTAGVVFVYANDKVERRTVTLGPTVGASRTVTSGVRDGERVVLSP
                     PESLEHGDAVQLAATP"
     gene            907..1647
                     /locus_tag="VLF19_04060"
     CDS             907..1647
                     /locus_tag="VLF19_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92458.1"
                     /translation="MSGDGRNPHGNGAGHDTPLVEIRGVTKIYRRGAEKIEVLHGIDL
                     DIPHGDFVALMGPSGSGKTTLLNLIGGLDTPTAGSIVVNGQRIDRMASGELSRWRAAN
                     VGFVFQFYNLLPVLSAQRNVELPLLLTSLPAAQRRRRALIALALVGLADRARHKPKEL
                     SGGQEQRVAIARAIVSDPTLLVCDEPTGDLDRATAAEILTLLQMLNREHGKTVVMVTH
                     DPKAAEYASRQLHLDKGTLAFVNGALAR"
     gene            1644..2810
                     /locus_tag="VLF19_04065"
     CDS             1644..2810
                     /locus_tag="VLF19_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92459.1"
                     /translation="MRYLPLLWAALFRKKARTVLTLLSVIVAFLLFGLLQAVQVAFES
                     GADTADAKRLLTTARYSIIEPLPQSYLRRIEQVPGVVGVAYADWFGAKYQNDSNAFPV
                     FAVDPARYLAMYPEFIIAPDQREAFARTRTGAVAGKRLVDRYGWKIGQKLPIASEIHP
                     RANGDLNWEFDLVGVLDADDPAVQGNTDVVLINVAYFDEARQFGKGKTGWYIVRIADS
                     TEARAISARIDALFANSPDETKTQPEKEFAIGFAKQIGDIGAMVTRILIAVFFTILIL
                     TGNTMAQSIRERIPELAVLKTLGFSDGKVTALVLAEALMLLLIGGGIGMAAAVGVLPA
                     VNSKTGGRFPPLFVGAETWLLALALVVALALTVGLPPALRARRLKIVDALAGHR"
     gene            2807..3991
                     /locus_tag="VLF19_04070"
     CDS             2807..3991
                     /locus_tag="VLF19_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92460.1"
                     /translation="MNQVVQITLINLRTIPQRLAPSLVVIIGIAGVVGVLVSVLAMAE
                     GFRHTLASTGRPNRVILLRAGSDAELSSGVPREQAALLGNLPGIARDAGGRPLASAEL
                     MVMVDLPRKGDPNPNNVPFRGVQPAAFAVRDELTLVEGRRFARGVREVIVGRKASQQF
                     AGLTVGSHLAFRDSDWRVVGVFATGGDVHESEIWADAEVAASAFRRQGFQSLTAALVD
                     GSDAGLEAFRHSIGRDPRFSINVFREPEYYARQAKVLSNLIDVLGYTVAVFMAIGATF
                     GALNCMYSAIASRQVEIATLRAIGFGGAPVVVSVMIEALVLAMLGGAVGGALAWLYCD
                     GASVSTLNFNTFSQVAFDFRVTPGLLAQGLLWALSIGAAGGLLPAVRAARLPVTVALR
                     TG"
     gene            complement(4013..4528)
                     /locus_tag="VLF19_04075"
     CDS             complement(4013..4528)
                     /locus_tag="VLF19_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92461.1"
                     /translation="MPTEFDKIKNFAGWKAKLKELLAAAEKASSRNDDDGRLSVATRL
                     NNFVLESVPNTDAIRALDELATAAAHELSVAVVEGAIERISHRTAALHSITKRLEAVS
                     LQAEQAAASIRLAKAHRVIDAFTEAVRAVDDLDAVLADGTDEQLRKRLATLARSMRVL
                     RTELDDSIGRV"
     gene            complement(4532..>5084)
                     /locus_tag="VLF19_04080"
     CDS             complement(4532..>5084)
                     /locus_tag="VLF19_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92462.1"
                     /translation="VAADTAAFIWAQVALARGAASLQEALGHAQPAIDRLAEIMSRDT
                     DDLAKIVRAMAVAQRTTLNETFNEAFGYRKTLARRRSILRDTGFGNLTEAELGELERI
                     EELMTPVNAELQKYDEQSVVIDRREKANLRLVAATRDSLERWAAAHREVALALQNRRP
                     VSVESLNGAAVELRALVRRIREL"
BASE COUNT          704 a         1883 c         1731 g          766 t
ORIGIN      
        1 ggccagggcc cagctcgcgc tggcccgggc gcagctcacc gcggcgcgct cgcagctcgc
       61 cgagatccag gcccagctcg agcaggccga gcgcgacttc gcgcgccagc aggagctgct
      121 ccggagggag ctcgtcgccg cgcagtccat ggagacggcc cgggcccagc gtgacacgct
      181 gcgcgcccgc ctggccgccg ccgaggagca gatcaaggtc gcgcagcagt cggtctcggt
      241 ggcccaggtg cagctcgaca acaccgagat ccgcgcgccg ttcacgggca tggtcgtggc
      301 caagtccgcg cagcccgggg agatgatctc gcccatctcc gccggcggcg gattcacccg
      361 caccgggatc gggaccatcg tcgacatgga ctcgctcgag atccaggtgg acgtcaacga
      421 ggcctatatc aaccgcgtca cgccgggaca gccggtgcag gccacgctga acgcctatcc
      481 ggactggaag atccccggct cggtgatcgc catcatcccg accgccgacc gtagcaaggc
      541 cacggtcaag gtacgcatcg ccatcaagca gagggacgcg cgcatcgtgc ccgacatggg
      601 cgcgcgggtg gcatttctcg agaaggagcc ggcgcgccgc gcctccccgg ccgaggccgg
      661 cgtgctcgtg cccgccgagg ccgtgcgcgg tgacggcacc gccggcgtgg tgttcgtgta
      721 cgccaacgac aaggtggagc gccgcacggt caccctgggg cccacggtcg gcgccagccg
      781 caccgtgacg agcggggtgc gcgacggcga gcgcgtggtg ctctcgccgc cggagtcgct
      841 ggagcacggc gacgccgtgc agctggcggc gacgccgtga gcgccaccgg cgcgagcggg
      901 aacgccatga gcggcgacgg ccgcaacccg cacggcaacg gcgccggcca cgacacgccc
      961 ctggtcgaga tccgcggcgt gaccaagatc tatcgccgcg gcgccgagaa gatcgaggtc
     1021 ctgcacggca tcgacctcga cattccccac ggcgacttcg tggcgctgat ggggccatcg
     1081 ggctcgggca agaccacgct gctcaacctc atcggcggtc tggacacgcc gaccgccggc
     1141 agcatcgtcg tgaacggcca gcggatcgac cggatggcga gcggcgagct gtcgcggtgg
     1201 cgagccgcca acgtgggctt cgtgttccag ttctacaacc tgttgccggt gctctcggcc
     1261 cagcgcaacg tcgagctacc gctgctgctg acctcgctcc cggccgcgca gcggcgccgc
     1321 cgcgccctga tcgcgctggc cctggtgggg ctcgccgacc gcgcccgtca caagccgaag
     1381 gagctctccg gcgggcagga gcagcgcgtg gccatcgccc gcgccatcgt ctccgacccc
     1441 acgctgctcg tctgcgacga gcccaccgga gacctcgacc gcgccaccgc cgccgagatc
     1501 ctgacgctgc tgcagatgct caaccgggag cacggcaaga ccgtcgtgat ggtcacgcac
     1561 gatcccaagg ccgccgagta cgccagccgc cagctccacc tcgacaaggg caccctggcc
     1621 ttcgtgaacg gagcgctcgc tcggtgaggt acctgccgct cctctgggcc gccctgttca
     1681 ggaagaaggc ccgcaccgtc ctgacgctgc tctcggtgat cgtggccttc ctgctcttcg
     1741 gcctgctgca ggcggtccag gtggccttcg agtccggcgc cgacacggcg gacgccaagc
     1801 gcctgctcac caccgcgcgc tactccatca tcgagcccct gccgcagtcc tacctgcgcc
     1861 gcatcgagca ggtgccgggc gtggtcggcg tggcctacgc cgactggttc ggcgccaagt
     1921 accagaacga cagcaacgcc ttcccggtgt tcgccgtgga ccccgcgcgc tacctcgcca
     1981 tgtacccgga gttcatcatc gccccggacc agcgcgaggc cttcgccagg acgcgcaccg
     2041 gcgccgtcgc cggcaagcgc ctggtggacc gctacggctg gaagatcggc cagaagctgc
     2101 cgatcgcctc ggagatccac ccccgggcca acggcgacct gaactgggag ttcgacctcg
     2161 tcggcgtcct cgacgcggac gacccggcgg tgcagggcaa caccgacgtc gtgctgatca
     2221 acgtcgccta cttcgacgag gcgcggcagt tcggcaaggg caagaccggc tggtacatcg
     2281 tccgcatcgc cgactccacc gaggcccggg ccatctcggc gcggatcgac gcgctgttcg
     2341 cgaactcgcc cgacgagacc aagacccagc ccgagaagga gttcgccatc ggcttcgcca
     2401 agcagatcgg cgacatcggc gcgatggtca cgcgcatcct catcgccgtg ttcttcacca
     2461 tcctgatcct gaccggcaac accatggccc agtcgattcg cgagcgcatc ccggagctgg
     2521 ccgtgctcaa gacgctgggc ttctccgacg gcaaggtgac ggcgctggtg ctggcggagg
     2581 cgctgatgct gctgctcatc ggcgggggca tcggcatggc cgcggcggtc ggcgtgctgc
     2641 ccgccgtcaa cagcaagacc ggcggccgct tcccgcccct gttcgtgggc gcggagacct
     2701 ggctgctggc gctcgccctc gtggtggcgc tggcgctgac cgtcgggctg ccgccggcgc
     2761 tccgcgcccg ccggctgaag atcgtggacg cgctggcggg acaccgatga accaggtggt
     2821 ccagatcacg ctgatcaacc tccggacgat tccccagcgg cttgcgccgt cgctcgtcgt
     2881 gatcatcggc atcgccggcg tcgtgggcgt gctggtgtcg gtgctggcga tggccgaggg
     2941 cttccgccac acgctggcca gcaccggacg cccgaaccgc gtcatcctgc tccgcgccgg
     3001 ctcggacgcc gagctgtcga gcggcgtgcc gcgcgagcag gccgcgctgc tcggcaacct
     3061 gcccggcatc gcgcgcgacg ccggcggacg ccccctggcc tcggccgagc tgatggtgat
     3121 ggtggacctg ccccgcaagg gcgaccccaa cccgaacaac gtccccttcc gcggcgtgca
     3181 gccggcggcc ttcgccgtcc gcgacgagct caccctcgtc gaagggcgtc gcttcgctcg
     3241 cggcgtgcgc gaggtcatcg tgggccgcaa ggcctcgcag cagttcgccg gcctcaccgt
     3301 cggctcccac ctggccttcc gtgacagcga ctggagggtg gtcggcgtct tcgcgaccgg
     3361 cggcgacgtg cacgagtccg agatctgggc cgacgccgag gtcgccgcct cggccttccg
     3421 gcgccagggg ttccagtcgc tcacggccgc gctcgtggac ggctcggacg cgggcctcga
     3481 ggccttccgt cactcgatcg gccgcgaccc tcgcttctcg atcaacgtct tccgcgaacc
     3541 ggagtattac gcgcggcagg ccaaggtgct gtcgaacttg atcgacgtgc tcggctacac
     3601 cgtggccgtc ttcatggcca tcggggcgac gttcggggcc ctcaactgca tgtactcggc
     3661 gatcgccagt cgccaggtcg agatcgccac cctgcgcgcc atcggcttcg gcggcgcccc
     3721 cgtcgtggtg tccgtgatga tcgaggccct ggtgctggcg atgctcggcg gcgccgtcgg
     3781 cggcgccctg gcctggctct attgcgacgg cgcctccgtc tccacgctga acttcaacac
     3841 cttctcccag gtcgccttcg acttccgcgt gaccccgggg ctgctggcgc agggactgct
     3901 gtgggcgctg agcatcggcg cggccggcgg cctgctgccg gcagtccgcg cagcgcggct
     3961 gcccgtgacc gtggcgctgc ggacgggcta gctggtcgga ggggccttcc gatcagaccc
     4021 gcccgatcga gtcatccagc tcggtccgga gcacgcgcat cgagcgggcg agcgtcgcga
     4081 ggcgcttgcg aagctgctcg tcggtgccgt ccgccaggac cgcgtccagg tcgtccacgg
     4141 cgcggacggc ctcggtgaag gcatcgatca cccggtgggc cttcgccagc ctgatcgatg
     4201 ccgccgcctg ctcggcctgc agcgacaccg cctccagcct cttggtgatg gagtgcaggg
     4261 ccgccgtgcg atgggagatg cgctcgatcg cgccctccac cacggccacg ctcagctcgt
     4321 gcgccgccgc tgtggccagc tcgtcgagcg cccggatggc gtcggtgttg ggcacggact
     4381 cgagcacgaa attgttcagc ctcgtcgcca cgctgagccg gccgtcgtcg tcgttcctgg
     4441 acgacgcctt ctcggccgcc gcgagcagct ccttcagctt ggccttccac ccggcgaaat
     4501 tcttgatctt gtcgaactcg gtgggcatcg ctcacagctc ccggatgcgc ctcacgaggg
     4561 cccgcagctc gacggcggcc ccgttcaacg actccacgct gaccggccgg cggttctgca
     4621 gggccagggc gacctcccgg tgggcggccg cccagcgctc cagcgagtcg cgggtggccg
     4681 cgacgagtcg gagattcgcc ttctcccgcc ggtcgatcac gacggactgc tcgtcgtact
     4741 tctggagctc ggcgttcacc ggcgtcatca gctcttcgat gcgctcgagc tctcccagct
     4801 cggcctccgt gaggttcccg aatcccgtgt cacgcaggat gctccgccgc cgggcgagcg
     4861 tcttccgata gccgaacgcc tcgttgaagg tctcgttgag cgtggtccgt tgcgccacgg
     4921 ccattgcccg gacgattttc gccaggtcgt cggtgtcgcg actcatgatc tcggccaggc
     4981 gatcgatcgc gggctgggcg tggccgagcg cctcctgtag cgaggccgcc cctctcgcca
     5041 gcgcgacctg cgcccagatg aacgccgcgg tgtcggccgc gacg
//
LOCUS       DATIAZ010000120         6125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_949901, whole genome shotgun sequence.
ACCESSION   DATIAZ010000120 DATIAZ010000000
VERSION     DATIAZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949901"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..3478
                     /locus_tag="VLF19_04085"
     CDS             77..3478
                     /locus_tag="VLF19_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSE92463.1"
                     /translation="MKCARCQHEHRPGAKFCEECAAPLDRTCASCGAPMSPTAKFCSE
                     CAHPAAQPPPSIPQRFGAPEPRFVAPDAYTPKHLAERILTSKAALEGERKQVTVLFAD
                     LKGSMELLADRDPEEARTLLDPVLEHMMEAVHRYEGTVNQVMGDGIMALFGAPLAHED
                     HAVRACYAALRMQESVKRYAADLQRSEGVPIAIRVGLNSGEVVVRAIGSDLRMDYSAI
                     GQTTHLAARMEQTAMPGSILVTADTLRLAEGYVRVAPLGPTRIKGLEAPVEVYEVIGA
                     SPTRTRLQASAARGLTRFVGRDAELDQIRRALERARAGHGQVVAVMGEPGVGKSRLFH
                     ELIRSHRTHGWLVLQASSVSYGRATLYLPVIELLRVYFGIQDRDDARAIRERVTGKIL
                     TLDEAFAEAIPPTLSLLEALPEDSPFRALDAGERRRRTLEALKRLVLREARIQPLLLV
                     FEDLHWIDAETQALLDSLVEGLATAPLLLLVNYRPEYRHGWGNKTCYAQLRLDPLAPE
                     DATQLLEALVGADPGLASLKRLLIERTEGNPFFLEESVRTMVETDVLVGERGAYRLAR
                     HGPIVQMPATVQAVLAARIDRLEPGDKTLLQMAAVIGKNVPLPLLRTTAREPEDTLLS
                     RLARLQAAEFLYEMSSFPDAEYTFKHALTLEVGYTSLLQERRRALHARVLEALESDTA
                     DGVYEHVERLAHHALGAERWDRAAVYLYQAGEKVFAYGAHASAAPFFEAAIDAVDRQG
                     GPGDRTLKLDACLELWAVRMETAQFHAVGALADTAEALATSLGDGPRLAKVRGGRAQM
                     LWAFPADTRALGAAIEHAREASSLADPADLRTRSYAQVVAGAALRDLGDLRGSIQEYD
                     RGTALFEGVEMVPAVQGFVRPIHSTLCSWRADGLVALGEFDRAMASGEEGRRVADEIG
                     HPSIRAQARSSLGLAHLMRGDVARSLTMLEPGLGIAEEAGAVFGILTNALGLAFARAL
                     LGDRGQAESHLERVREVGARLGRVTTKLTRYGGLVAATLLALGRAEEARAEVDQGLAL
                     VAAHAARGYEPTLLWLRAEALSSQAPDALGEALRSCEQALGLAQRLEMRPEIGHCHRV
                     MSRLLRRADDEASARNHLEAALALFRELGMPFWADRVQPEQARTQ"
     gene            complement(3678..5996)
                     /locus_tag="VLF19_04090"
     CDS             complement(3678..5996)
                     /locus_tag="VLF19_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE92464.1"
                     /translation="MPPAVGRSVLRKEDRRLLMGRGKYAADFRLPGMLHAAVLRSPHA
                     HARLGAIRADRALGLPGVVAVVTAADLGDIGRIPTRPGHRVGSVACLQRPLTRDRVRY
                     VGEPIALVIAESRYVAEDAVDLLEVDYDPLPAVTDARRAVERDAPVLHDALGGNVVDT
                     IEMRKGDAAAAMASAHTRVRERLAVQRHTGVPLETRGLTAAFDPGTGLLRLWGVAKLP
                     HFNRRVLADLLGHPEHLIHFVELEVGGGFGVRGEFYPEDLLVPWAAMRLRRPMQWIED
                     RREHLMAANHSRQQWHETEIGFDRDGRIVALIDRFITDMGAWLRTHGVVVPELTAALL
                     PGPYRIPHYTSEARCVLTNKTPTGTYRGPGRFEGTFVRERLMDIAAARLGLDPLELRR
                     RNFIAPDEMPYDVGGAALAQHTVYDCGDYASALGHALEAIGYEAARAEQTDARRQGRH
                     VGIGVASMVEKAGLGPWEYARVEVDATGHVVVYSGVASVGQGIETTLAQVCADELNVP
                     PESITVVHGDSARVPFGIGGFASRGASVALPAALEAARKVRAKIVLVAAGLLEAVAED
                     LVLEGGAVHVRGVPDRKVTFRDLARAAVPGPPGMEPGLHAAHFFEAPRMTYPYGTHAA
                     MVEVDAGTGVVKILRYAIAWDVGKAVNPMIVDGQLVGALAQGIGGALLEEIVYDEAGQ
                     LLTGSLMDYLLPTAMDMPEATEVRALEETPTPLNPLGIKGVGEGGSSACGGALANAVA
                     DALAPLGVSIVELPLSPGRLSALIRAAGPKRP"
     gene            complement(6002..>6125)
                     /locus_tag="VLF19_04095"
     CDS             complement(6002..>6125)
                     /locus_tag="VLF19_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSE92465.1"
                     /translation="PSDAQIREATSGNLSRCTGYKNIVTAARAAARALAAPTRR"
BASE COUNT          837 a         2248 c         2200 g          840 t
ORIGIN      
        1 cccatctgtc cgcgggagtg gtactaatgc ggcgccctgg gccagcgcgc acatcgccgc
       61 tcggacgcgg ctcgccatga agtgcgcgcg gtgccagcac gagcaccgtc ccggcgcgaa
      121 gttctgcgag gagtgcgccg cgcccctgga ccgcacgtgc gccagctgcg gcgcaccgat
      181 gtccccgact gcgaagttct gctcggagtg cgcccatccg gccgcccagc ctcccccctc
      241 gatcccccag cgcttcggcg cgccggaacc acgtttcgtg gcgccggacg cctatacccc
      301 caagcacctc gccgagagga tcctgacgtc gaaggccgcg ctcgaaggcg agcggaagca
      361 ggtgacggtg ctcttcgctg acctcaaggg atccatggag ctgctggccg accgtgatcc
      421 ggaagaggcg cggacactcc tcgatccggt gctggagcac atgatggagg cggtccaccg
      481 ctacgagggc accgtcaacc aggtgatggg cgacggcatc atggcgctgt tcggagcccc
      541 gcttgcccac gaggatcacg ccgtccgcgc ctgctacgcc gcgcttcgga tgcaggagtc
      601 ggtgaagcgc tacgcggcgg acctccagcg gagcgagggc gtcccgatcg ccatccgcgt
      661 cggcctgaac tcgggcgagg tggtggtgcg cgcgatcggg tccgacctcc gcatggacta
      721 cagcgccatc gggcagacca cacacctcgc cgcacgcatg gagcagacgg cgatgcctgg
      781 ctccatcctc gtcaccgccg acacgctgcg gctcgccgag ggctatgtcc gcgtggcccc
      841 gctcgggccg acccgcatca aggggctgga agccccggtg gaggtgtacg aggtcatcgg
      901 cgcaagcccc acgcggacgc ggctacaggc ctcggccgcg cgcgggctca cgcgcttcgt
      961 cggccgcgat gccgagctcg atcagatccg cagggcgctc gagcgagcgc gtgcgggcca
     1021 cggccaggtc gtcgccgtca tgggcgagcc cggtgtcggg aagtcgcgcc tgttccacga
     1081 gctgatccgc tcgcaccgca cccatggctg gctcgttctc caggcgagct cggtgtcgta
     1141 tggccgggcg acgctctacc tcccggtcat cgagctcttg cgggtctact tcgggatcca
     1201 ggaccgtgac gacgcgcggg ccatccggga acgcgtgacg ggcaagatcc tgactctcga
     1261 cgaggcgttc gccgaggcca tccctcccac gctctcgctg ctggaggcgc tccccgagga
     1321 cagccccttc cgcgcgctcg atgccggcga gcgccggcgg cgcacgctgg aggcgctcaa
     1381 gcggctggtg ctccgcgagg cgcggatcca gcccctgctc ctcgtcttcg aagacctcca
     1441 ttggatcgac gccgagaccc aggcgctcct cgacagcctg gtcgagggcc ttgcgacggc
     1501 gccgctgctc ctcctcgtca actaccggcc cgagtatcgc cacggctggg gcaacaagac
     1561 ctgctacgcg cagctccggc tggaccccct cgcccccgag gatgcgacgc agctcctcga
     1621 ggccctcgtg ggcgcggatc cgggactcgc ctcgctcaag cggctcctga tcgagcggac
     1681 ggaagggaac cccttcttcc tggaggagag cgtgcgcacc atggtcgaga cggacgtgct
     1741 cgtcggcgag cgtggcgcct accggctggc ccgccacggg cccatcgtcc agatgcccgc
     1801 cacggtgcag gcggtgctcg cggctcgcat cgaccggctc gagcccgggg acaagaccct
     1861 gctccagatg gccgcggtga tcggcaagaa cgtccccctg cccctcctgc ggaccaccgc
     1921 aagggagccc gaggacaccc tgctgagccg gctcgcgcgc ctacaggccg ccgagttcct
     1981 gtacgagatg agctcgttcc cggacgccga gtacacgttc aagcacgcgc tgaccctgga
     2041 ggttggctac acgagccttc tccaggagcg ccggcgcgcg ctgcacgcgc gagtgctgga
     2101 agcgctggag agcgacacgg cggatggggt ctacgagcac gtggagcggc tggcccatca
     2161 cgcgctcggc gccgaacggt gggatcgcgc cgccgtctac ctctaccagg cgggcgagaa
     2221 ggtgttcgcc tacggcgcgc atgcgtcagc cgcgccgttc ttcgaggctg ccatcgacgc
     2281 ggtggaccgc cagggcgggc cgggcgatcg gaccctcaag ctcgacgcgt gcctcgagct
     2341 gtgggcggtc aggatggaga ccgcccagtt ccacgcggtc ggagcgctgg ccgacaccgc
     2401 ggaggcgctg gcgacgtcgc tcggcgatgg gcctcgcctc gcgaaggtcc gcggcggccg
     2461 ggcgcagatg ctgtgggcct tcccggccga cacccgggcg ctcggcgcgg ccatcgagca
     2521 tgcgcgggag gcttcctcgc tggccgatcc cgccgatctc aggacgcgga gctacgcgca
     2581 ggtcgtggcc ggcgccgcgc tccgggatct cggtgacctc cggggctcga tccaggaata
     2641 cgaccgcggc acggccctgt tcgaaggcgt cgagatggtc ccggccgtgc aggggttcgt
     2701 gcgcccgatc cactcgaccc tctgctcgtg gcgagcggac ggcctggttg cactgggcga
     2761 gttcgaccgc gcgatggcgt ccggagagga gggtcgtcgc gtggccgatg agatcggcca
     2821 tccctcgatc cgcgcccagg cgcgcagctc cctgggtctc gcgcacctga tgcgcgggga
     2881 cgtcgcccga tcgctcacaa tgctcgagcc ggggctcgga atcgccgaag aggccggcgc
     2941 cgtcttcggg atcctgacga acgcgctggg cctcgccttc gcccgggcac tgctcggcga
     3001 ccgtggacag gcggagagcc acctcgagcg ggtgcgtgag gtcggtgccc ggctcggccg
     3061 cgtgacgacg aagttgacgc ggtacggcgg cctcgtcgcc gcgaccctgc tcgccctggg
     3121 ccgcgccgag gaggcccgcg ccgaggtcga ccaaggcctg gcgcttgtcg ccgcccatgc
     3181 cgcgcgcggg tacgagccca cgctcctctg gctgcgtgcc gaggccctct catcgcaggc
     3241 tcctgacgct ctcggggaag ctctccgctc gtgcgagcag gccctggggc tcgcccagcg
     3301 actcgagatg cgcccggaga tcggccattg tcaccgcgtg atgagccggc tcctgcggcg
     3361 cgccgacgac gaggcgagtg cccgcaacca cctcgaggct gcgctcgccc tcttccggga
     3421 gctgggcatg ccgttctggg cggatcgcgt tcagcccgag caagcgcgta cgcagtgaag
     3481 ctgcggcccg ccgaccggtc accgcgtgcc gagtgggtcg gccgcattcc ccggcacggc
     3541 gccacactgg gtgcagagct cgaggcgggc gcacggttcg tgacttcgag ggcgacccgg
     3601 cgccgtgcgg cgcctctcta ggcgcgggcc gccattggta gggagcgggc gcctgcggct
     3661 cacgtcgcgc cctaacgtca cggacgcttc ggccccgccg cgcggatcag ggctgacagg
     3721 cggccgggag acagcggcag ctcgacgatc gagacgccga gcggcgcgag cgcgtcggcc
     3781 accgcgttgg cgagcgcgcc gccgcacgcc gagctgccgc cctcgccgac gcccttgatg
     3841 ccgagcgggt tgagcggcgt cggggtctcc tccaaagcgc ggacctccgt ggcctccggc
     3901 atgtccatcg ccgtcggcag caggtagtcc atgagcgacc cggtgagcag ctgccccgcc
     3961 tcgtcgtaca cgatctcctc gaggagcgcg ccgccgatcc cctgcgcgag ggcaccgacc
     4021 agctggccgt cgacgatcat cggattcacc gccttgccga cgtcccacgc gatcgcgtac
     4081 cggaggatct tcacaacgcc ggtgccggcg tccacctcga ccatggccgc gtgcgtcccg
     4141 taggggtagg tcatcctcgg cgcctcgaag aagtgcgcgg cgtggagtcc cggctccatc
     4201 cccggcgggc cggggacggc ggcccgcgcg agatcgcgga acgtgacctt gcggtccggc
     4261 acgccgcgca cgtgcaccgc gcccccctcg agcacgaggt cctcggccac ggcctcgagg
     4321 agaccagccg ccacgagcac gatctttgcg cggaccttcc gcgccgcctc cagcgccgcc
     4381 ggcagcgcga ccgaggcgcc gcggctcgcg aagccgccga taccgaaggg cacccgtgca
     4441 ctgtcgccgt ggacgacggt gatggactcg ggcggcacgt tcagctcgtc cgcgcagacc
     4501 tgggcgagcg tggtctcgat cccctgcccg accgaggcga ccccggagta cacgacgacg
     4561 tgcccggtcg cgtcgacctc gacccgcgcg tactcccagg gcccgagccc ggccttctcg
     4621 accatgctgg ccacgccgat cccgacgtgg cgcccctgcc gccgcgcgtc ggtctgctcc
     4681 gcacgcgccg cctcgtagcc gatggcctcg agggcgtggc cgagcgccga ggcgtagtcg
     4741 ccgcagtcgt agacggtgtg ctgggccagc gcggcgccgc cgacgtcgta cggcatctcg
     4801 tcgggcgcga tgaagttgcg gcgcctcagc tcgagcggat cgagcccgag ccgcgccgcc
     4861 gcgatgtcca tgagccgctc gcgcacgaac gtgccctcga agcggcccgg tccccggtag
     4921 gtgcccgtcg gggtcttgtt ggtcagcacg caccgggcct ccgacgtgta gtgcggaatc
     4981 cggtaggggc cgggcaggag cgcggccgtg agctcgggga cgacgacgcc gtgcgtgcgc
     5041 agccaggcgc ccatgtcggt gatgaaccgg tcgatcaggg ccacgatccg cccgtcgcgg
     5101 tcgaacccga tctctgtctc gtgccactgc tggcgcgagt ggttcgccgc catgagatgc
     5161 tcgcgacggt cctcgatcca ctgcatcggc cgccgcaacc gcatcgcggc ccacgggacc
     5221 aggaggtcct ccggatagaa ctcgccccgc acgccgaagc cgccgcccac ctccagctcg
     5281 acgaagtgga tcagatgctc cggatggccc aggaggtcgg cgaggacgcg ccggttgaag
     5341 tgagggagct tggcgacccc ccacagccgc agcagcccgg tgccggggtc gaacgccgcg
     5401 gtcaggccgc gcgtctccag cgggaccccg gtgtgccgct ggacggcgag gcgctcgcgc
     5461 acgcgcgtgt gcgcggaggc catggcggcg gccgcatcgc ccttgcgcat ctcgatcgtg
     5521 tccaccacgt tcccgccaag ggcgtcgtgg agcacgggcg catcgcgctc gacggcccgg
     5581 cgcgcgtcag tgaccgcggg gaggggatcg tagtctacct ccagcaggtc gacggcgtcc
     5641 tcggcgacgt accggctctc ggcgatcacc aaggcgatcg gctcgcccac gtaacgcacg
     5701 cggtcacgag tgagcggtcg ctgcaggcag gccacgctgc cgacccggtg cccgggccgt
     5761 gtcgggatgc gcccgatgtc gccgaggtcg gcggcggtca cgacggcgac cacccccggc
     5821 aggccgagcg cccggtcggc gcggatggcg ccgaggcgcg cgtgcgcgtg gggggaccgg
     5881 aggacggcgg cgtgcagcat gccggggagc cggaagtcgg ccgcatactt gccgcggccc
     5941 atgagcagcc ggcggtcttc cttgcgcagc acggagcgtc cgaccgcggg gggcatcggc
     6001 gtcagcgcct cgtcggggcg gccagcgccc gcgcggccgc gcgggccgcc gtcacgatgt
     6061 tcttgtagcc ggtgcagcgg ctcaggttgc cggaggtcgc ctcgcggatc tgcgcgtccg
     6121 acgga
//
LOCUS       DATIAZ010000121         4242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_293430, whole genome shotgun sequence.
ACCESSION   DATIAZ010000121 DATIAZ010000000
VERSION     DATIAZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293430"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VLF19_04100"
     CDS             <1..512
                     /locus_tag="VLF19_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92466.1"
                     /translation="TVAPARHACAIDDRAVVEAFPNLFLGVLCDDADYPARPRRARRW
                     TDDLYELQRPRTKMRRRLAGLVSALTRPRAAAGGPSRRAIAGSWDLRDHDERAGLVCA
                     LAALCVAAGRFVAVGSPKDGWIALPPADHWGRGEGREPWAWRVLRDNLVAVAADFPDA
                     AVARHGEAT"
     gene            525..3113
                     /gene="polA"
                     /locus_tag="VLF19_04105"
     CDS             525..3113
                     /gene="polA"
                     /locus_tag="VLF19_04105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HSE92467.1"
                     /translation="MSRFYIVDGHSHLFRAYHAVGYLSTSKGVPSHAVLILSNMLWKL
                     VREEQPEYLGVAWDPPGPTFRDALFTEYKATRTAMPGDLARQIPYVRRLFEALHTPVV
                     EVPGFEADDVLATLVDRALALDVEVVIVTGDKDLLQLVGPRVRVMSVIGRTGEPIVYD
                     EAKVRERWGVEPSQIADVLALMGDSIDNIPGVHGVGEKTAVKLIGQFGSVARLYENLT
                     LVPGKLRETLAAGRKDALLSRELAVLNHEVPVDLDLERFRRVEPDWTKLRALWMELEF
                     TRLIKELPAQAPAVREAVEAAPALATPESLAAWLARVPAGAPIAVDWTGLARPPRPRI
                     AGLGLFHPAAGRAWVANDGTLPDLGQHPLVVHDAKAVVQRRLADGQPPPAVEDSAVAA
                     YLLNPARATYRLDEVCMEAFADCPPTLPPELPAAVDQALGDVLAERARWLWRYWEHAA
                     AALDAQGLRALYDDIERPLVAVLALMERHGIRVEPARLEAFAKELERQLDSLQRDVWR
                     LAGQEFNIGSPKQLAQVLFEKLKLPVVRRTKTGPSTDADVLTELAAGHELPAKVLEHR
                     SLAKLKGTYADTLPALIDPGTGRIHTTFNQLVAATGRLSSQDPNLMNIPIRTELGRRI
                     RAAFVPEEGWRFVAADYSQIELRILAHLSGEPAIIEAFERGEDIHTRTACEVFKVAPK
                     DVTSLQRTIAKSANYAILYGVSAFGLSQATRIDQKEAQRYIAAYFATHPRVRAFIDGT
                     LAEGRERGYVSTLLGRRRYLPDLNSGNPVARNAAERMAMNAPVQGSASDMIKIAMVRM
                     HRALAERGLRARMLLQVHDELLFEAPPAEVATVEALAREIMAAALPLRVPVVVDVKSG
                     TDWSEV"
     gene            3110..3745
                     /gene="coaE"
                     /locus_tag="VLF19_04110"
     CDS             3110..3745
                     /gene="coaE"
                     /locus_tag="VLF19_04110"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097474.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSE92468.1"
                     /translation="MTRPFLLAGLTGGIATGKSAVTAMFRALGAVVIDADVLAREVVE
                     PGEPALGEIVREFGRGVLQPDGRLDRKALGAIVFADAGKRRRLEAITHPPIRDRFLRH
                     LVDLEARGFHGIVFFDAPVMIESGNYKNMDRLVVVVTDEATQVARLMARDGLSREEAL
                     GRIRSQMPVAEKAKLADYVIDNSGDRTQTDAQVRRCHERLLDDLARRGVAR"
     gene            3984..4154
                     /locus_tag="VLF19_04115"
     CDS             3984..4154
                     /locus_tag="VLF19_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92469.1"
                     /translation="MLHRIQKLVHDKAMYAPIWELGFIHAQGPRVAESGLGLITGWAF
                     SAPYEDVQLKGT"
BASE COUNT          598 a         1557 c         1516 g          571 t
ORIGIN      
        1 ccaccgtcgc acccgcccgc cacgcctgcg cgatcgatga ccgcgcggtg gtcgaggcat
       61 tccccaacct ctttctcggc gtgctgtgcg acgacgcgga ctatcccgcc cggccccgcc
      121 gggcgcgccg gtggaccgac gatctctacg agctgcaacg cccgcggacg aagatgcgga
      181 ggcggctggc aggactggtg tcggccctca cccgcccgcg agcggccgcc ggcggcccga
      241 gccggcgggc gatcgccggg agctgggacc tccgcgacca cgacgagcgg gcggggctcg
      301 tgtgcgcgct ggccgctctc tgcgtcgcgg ccgggcggtt cgtcgccgtc gggtcgccga
      361 aggacggctg gatcgccctg ccgcccgcgg accactgggg ccgcggcgag ggacgcgagc
      421 cctgggcgtg gcgggtgctc cgggacaacc tcgtcgcggt ggcagccgac ttccccgacg
      481 ccgccgtggc gcgccacggc gaggccacat agaatgacgg cgacgtgagt cgcttttaca
      541 tcgtcgacgg ccactcgcac ctgttccgcg cctaccacgc ggtcggctac ctctcgacct
      601 ccaagggcgt gccgagccac gcggtgctga tcctgagcaa catgctctgg aagctcgtgc
      661 gcgaggagca gccggagtac ctcggcgtcg cctgggaccc gcccggcccc acgttccgcg
      721 acgcgctctt caccgagtac aaggccacgc gcacggcgat gcccggtgac ctggcgcgac
      781 agatcccgta cgtgcggcgc ctcttcgagg ccctccacac cccggtcgtc gaggtccccg
      841 gcttcgaggc cgacgacgtg ctggcgacgc tggtcgaccg ggcgctcgcc ctggacgtcg
      901 aggtcgtcat cgtcaccggc gacaaggatc tgctccagct cgtggggccg cgcgtccgcg
      961 tgatgagcgt gatcgggcgc accggcgagc ccatcgtgta cgacgaggcg aaggtgcgcg
     1021 agcgctgggg cgtggagccc tcgcagatcg ccgacgtgct cgcgctgatg ggcgactcga
     1081 tcgacaacat tcccggcgtg cacggcgtcg gcgagaagac cgcggtgaag ctgatcggcc
     1141 agttcggcag cgtcgcgcgg ctctacgaga acctcacgct ggtgcccggc aagctcaggg
     1201 agacgctggc ggccgggcgc aaggacgcgc tgctgtcgcg cgagctggcc gtgctcaacc
     1261 acgaggtgcc ggtcgacctc gacctcgagc gcttccggcg cgtcgagccc gactggacca
     1321 agcttcgcgc gctctggatg gagctggagt tcacgcgcct gatcaaggag ctgccggccc
     1381 aggcgccggc ggtgcgcgaa gccgtggagg ccgcgccggc gctcgccacg ccggagtcgc
     1441 tggccgcatg gctcgcccgg gtcccggccg gcgctccgat cgccgtggac tggacgggcc
     1501 tggcgcggcc gccgcggccc cggatcgcgg gcctcgggct cttccacccc gccgccggcc
     1561 gggcctgggt ggctaacgac ggtacgctgc cggacctcgg ccagcatccg ctcgtcgtcc
     1621 acgacgccaa ggcggtcgtg cagcgccggc tcgccgacgg ccagccgccg cctgccgtcg
     1681 aggacagcgc tgtcgccgca tatctcctga accccgcgcg ggccacgtat cggctggacg
     1741 aagtgtgcat ggaggcgttc gccgactgcc cgccgacgct gccgcccgag ctgccggccg
     1801 cggtcgacca ggcgctcggt gacgtgctgg cggagcgcgc gcggtggttg tggcgctact
     1861 gggagcacgc cgccgcggcc ctcgatgctc aggggctgcg cgcgctgtac gacgacatcg
     1921 agcgcccgct cgtcgccgtg ctcgccctca tggagcgcca cggcatccgc gtggagccgg
     1981 cgcggctcga agccttcgcc aaggagctgg agcgccagct cgattcgctg cagcgcgacg
     2041 tctggcgcct ggcggggcag gagttcaaca tcggctcgcc gaagcagctg gcgcaggtgc
     2101 tgttcgagaa gctgaagctg ccggtggtgc gccgtaccaa gacgggtccc tcgaccgacg
     2161 ccgacgtgct gaccgagctg gcggcgggcc acgagctgcc cgccaaggtg ctcgagcacc
     2221 gcagcctcgc caagctgaaa ggcacatacg ccgacacgct gccggcgctc atcgaccccg
     2281 ggacgggacg catccacacg acgttcaacc agctcgtcgc ggcgacgggc cggctctcga
     2341 gccaggatcc gaacctcatg aacatcccga tccgcacgga gctcggccgg cggatccggg
     2401 cggccttcgt ccccgaggag ggctggcggt tcgtcgccgc cgactactcg cagatcgagc
     2461 tgcgcatcct cgcccacctg tccggggagc cggccatcat cgaggccttc gagcgcggcg
     2521 aggacatcca cacccgcacc gcctgcgagg tgttcaaggt cgcgccgaag gatgtcacgt
     2581 cgctccagcg gacgatcgcc aagagcgcga actacgccat cctctacggc gtcagcgcct
     2641 tcgggctcag ccaggccacg cggatcgatc agaaggaggc gcagcgctac atcgccgcgt
     2701 acttcgccac ccacccgcgc gtgcgcgcct tcatcgacgg cacgctcgcc gagggccgcg
     2761 agcgcggcta cgtctcgacg ctgctcggtc gccgccgtta cctgccggac ctcaacagcg
     2821 gcaaccccgt cgcccgcaac gccgccgagc ggatggcgat gaacgcgccg gtgcagggca
     2881 gcgccagcga catgatcaag atcgccatgg tgcgcatgca ccgcgcgctc gccgagcgcg
     2941 ggctccgcgc gcggatgctg ctgcaggtgc acgacgagct gctcttcgag gcgccgcccg
     3001 cggaggtcgc caccgtggag gcgctcgcgc gcgagatcat ggccgcggcg ctgccgctcc
     3061 gcgtgccggt ggtcgtggac gtcaagtcgg gcaccgactg gtccgaggtg tgacgcgccc
     3121 gttcttgctc gccgggctca ccggcggcat cgcgaccggc aaatccgccg tgaccgcgat
     3181 gttccgcgcc ctgggcgccg tggtcatcga cgccgacgtc ctggcgcgcg aggtggtcga
     3241 gcccggcgag ccggcgctgg gcgagatcgt ccgcgagttc ggtcgcggcg tgctccagcc
     3301 cgacggacgg ctcgaccgca aggccctcgg cgccatcgtc ttcgccgatg ccggcaagcg
     3361 ccgccggctc gaagccatca cgcacccgcc catccgcgat cgcttcctgc gacatcttgt
     3421 cgatctcgag gcgcgaggct tccacggcat cgtcttcttc gacgcgccgg tcatgatcga
     3481 gagcggcaac tacaagaaca tggaccggct cgtcgtcgtc gtgaccgacg aggccaccca
     3541 ggtcgcgcgg ctgatggccc gcgacggcct ctcgcgcgag gaggcgctcg gccggatccg
     3601 cagccagatg ccggtcgccg agaaggccaa gctcgccgat tacgtgatcg acaactccgg
     3661 cgaccgcacg cagaccgacg cccaggtgcg tcggtgccac gagaggctcc tcgacgacct
     3721 cgctcgccgg ggcgtggccc gctgagcccg cggcgaacag gcggcgagcc cgccgggctc
     3781 cgatggggcg cgacggccgg ttggacggcg attgacgcgc ccagcctcgc ggactagcct
     3841 tggcgaactt ggtgagaccc ggcaggcagt gtccgacggc cggacgctgg cgaaaccgaa
     3901 ggtgctggcg gggaagtatc ccgacatcga cgggctcttc cgggagcagg ctggcgagct
     3961 cgaccggaag cggcggggag gcgatgctcc accggatcca gaagctcgtc cacgacaagg
     4021 cgatgtacgc gccgatctgg gagctgggct tcatccacgc gcagggcccc cgggtggccg
     4081 agtcgggcct cgggctcatc acgggctggg cgttctccgc gccgtacgag gacgtccagc
     4141 tgaaggggac gtgatccgcc gccgtgccct cggccagcac ttcctgcgcg acgccggcat
     4201 cgcgcgcgcg atcgtcgatc tggtcgcgcc ggccgagcgc ga
//
LOCUS       DATIAZ010000122         3163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_687856, whole genome shotgun sequence.
ACCESSION   DATIAZ010000122 DATIAZ010000000
VERSION     DATIAZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687856"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /gene="bcp"
                     /locus_tag="VLF19_04120"
     CDS             complement(<1..448)
                     /gene="bcp"
                     /locus_tag="VLF19_04120"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSE92470.1"
                     /translation="MAAVAEARKAPSFTLEDAAGKQVSLADFKGKHVVVYFYPKDDTP
                     GCTKEACGFRDLWRELQRAGVVVLGISADDATSHTRFSEKYKLPFTLLSDPDRKVMQA
                     WGAYGDKMMYGRKVKGVIRSTVWVGPDGVVRKHWTRVRDAARHPEEV"
     gene            complement(457..960)
                     /locus_tag="VLF19_04125"
     CDS             complement(457..960)
                     /locus_tag="VLF19_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSE92471.1"
                     /translation="MAIKVGDRLPEGTLTEMIDTERPGCTLGPNNFSVQDLTKGTRIV
                     IFGLPGAFTPTCSAKHVPSYVQNIDKLKGKGVDEVWCVAVNDAFVMGAWGKDQKCDGK
                     VRMMADGSATFTKALGLELDLVARGMGVRSQRYSMLVEDGVVKALNVEQPGKFEVSGA
                     EAMLQKL"
     gene            complement(1014..2561)
                     /locus_tag="VLF19_04130"
     CDS             complement(1014..2561)
                     /locus_tag="VLF19_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HSE92472.1"
                     /translation="MDPVARHPFAPDASFDGGDLDCGGGLLLLIRRHMDPLPRGGLLE
                     IRSTETSVDQDLPAWCRMTGNELVSWTREGASRSFLVSKGRLAERQGAPPGAPSVSGA
                     PAGTPRLVAGHRVVPVTVPSSLPPPAPAPEIPALAVMGIGSWPRPRWMLQTLHERLEG
                     RLAEEEFQATADDAVRLAVDAQIRAGVDVISDGEQRRDNYASFIGARLDNCQLIPLTD
                     LLPLVDDPDEFEREMQSLDVPASQVRHPAVLGPLGRSRPLAGHEHEFLRTLTDRPAKV
                     ALPGPYLMTRLMWLPCVSDRVYRSREHLAEDVVRVLREELHFLLAAGVALVQLDEPVL
                     SEVVFTGPRNTRSFMCGALSEKGDAAKELDFARTLLGRVLEGLPRRRLALHVCRGNWT
                     PDERAALAGDYRPLLPLLADVPVGTLFLELATPRAGEIEVLRDLPRECRIGVGVVNQK
                     HERIEPEEEIAARARRAIALFGAERVLLTPDCGFATFADNPVASARVAEAKLRAVAAA
                     AERLRRE"
     gene            complement(2660..3022)
                     /locus_tag="VLF19_04135"
     CDS             complement(2660..3022)
                     /locus_tag="VLF19_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSE92473.1"
                     /translation="MANKFCVSLTCAKDNSDKATVAFVVANAALGSGKETLVFLSVEG
                     VRLSQKGYADAIHEEGFAPLRELMDNFAKGGGTMYVCAPCFKKRKLDEHSLVAGAAVV
                     GGAKLVEFLSDGSPCVSY"
     gene            complement(3026..3151)
                     /locus_tag="VLF19_04140"
     CDS             complement(3026..3151)
                     /locus_tag="VLF19_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92474.1"
                     /translation="MLELVARDPGAPADLPAWCRLTGHALVEQRPPVYRIRRKES"
BASE COUNT          444 a         1099 c         1151 g          469 t
ORIGIN      
        1 agacctcctc ggggtgcctg gccgcgtcgc ggaccctcgt ccagtgcttg cgcacgacgc
       61 cgtcggggcc cacccagacc gtcgagcgga tgacgccctt caccttcctg ccgtacatca
      121 tcttgtcgcc gtacgcgccc cacgcctgca tgaccttgcg gtcggggtcg gagagcagcg
      181 tgaagggcag cttgtacttc tccgagaacc gggtgtgcga ggtggcgtcg tccgcggaga
      241 tgccgaggac gacgacgccg gcgcgctgca gctcccgcca gaggtcgcgg aagccgcacg
      301 cctccttggt gcagcccggc gtgtcgtcct tggggtagaa gtagacgacg acgtgcttgc
      361 ccttgaagtc cgcgagcgac acctgcttgc ccgccgcgtc ctccagggtg aagctcgggg
      421 ccttccgggc ttcggcgacg gcggccacgg cgggcctcag agcttctgca gcatcgcctc
      481 ggcgccgctg acctcgaact tgccgggctg ctccacgttg agcgccttga ccacgccgtc
      541 ctcgaccagc atcgagtacc gctgggagcg cacgcccatg ccgcgggcga cgaggtccag
      601 ctccaggccg agcgccttgg tgaacgtggc gctgccatcg gccatcatgc gcaccttgcc
      661 gtcgcacttc tggtccttgc cccaggcacc catgacgaag gcgtcgttga cggcgacgca
      721 ccagacctcg tccacgccct tgcccttgag cttgtcgatg ttctgcacgt agctcggcac
      781 gtgcttggcc gagcacgtcg gcgtgaaggc cccgggcagg ccgaagatga cgatgcgcgt
      841 gcccttggtg aggtcctgca cggagaagtt gttggggccg agggtacagc cgggacgctc
      901 ggtgtcgatc atctcggtca gcgttccctc gggcagccgg tcgcctacct tgatggccat
      961 gtcgtcctcc tggatcgttg ctgggtaccg gcgacgatgc cggggatgat acgtcattcc
     1021 cgccgcaagc gctcggcggc ggcggcgacg gcgcgcagct tggcctccgc cacgcgcgcg
     1081 gaggcgaccg ggttgtcggc gaaggtggcg aagccgcagt ccggggtgag cagcacgcgc
     1141 tcggcaccga agagtgcgat ggcgcgccgg gcgcgcgccg cgatctcctc ctcgggctcg
     1201 atccgctcgt gcttctggtt cacgacgccc acgccgatcc ggcactctcg gggcaggtcg
     1261 cggagcacct cgatctcgcc ggcgcggggc gtggccagct cgaggaagag cgtgcccacg
     1321 ggcacgtcgg cgagcagggg caggagcggc cggtagtcgc cggccagggc ggcgcgctcg
     1381 tcgggcgtcc agttcccgcg gcagacgtgg agcgcgagcc ggcggcgggg caggccctcg
     1441 agaacgcggc cgagcagcgt ccgcgcgaag tccagctcct tggcggcatc gcccttctcg
     1501 ctgagcgcgc cgcacatgaa gctccgcgtg ttcctggggc cggtgaagac gacctccgag
     1561 agcacgggct cgtcgagctg gacgagggcg acaccggcgg cgagcaggaa gtgaagctcc
     1621 tcgcgcagca cgcgcacgac gtcctcggcc aggtgctcgc gcgagcggta gacgcggtcg
     1681 gacacgcacg gcagccacat gagccgggtc atcagatacg gtccgggcag ggcgaccttc
     1741 gccgggcggt cggtgagcgt ccggaggaac tcgtgctcgt gtccggcgag cgggcggctg
     1801 cgcccgagcg ggccgagcac ggccgggtgg cgcacctggg acgcgggcac gtcgagcgac
     1861 tgcatctcgc gctcgaactc gtcgggatcg tcgacgagcg gcagcaggtc ggtgaggggg
     1921 atgagctggc agttgtcgag ccgcgcgccg atgaacgacg cgtagttgtc gcggcgctgc
     1981 tcgccgtcgc tgatcacgtc gacgcccgcg cggatctggg cgtcgacggc gaggcgaacg
     2041 gcgtcgtccg ccgtcgcctg gaattcctcc tcggccaggc gaccttcgag gcgctcgtgg
     2101 agcgtctgca gcatccaccg cggacgcggc cagcttccga tacccatcac ggccagcgcg
     2161 gggatctccg gagccggcgc gggcgggggc agcgaggacg ggaccgtcac cggcaccacg
     2221 cggtggccgg ccacgaggcg tggcgtcccg gctggcgccc cggagacgga gggcgccccg
     2281 ggtggcgccc cctggcgctc ggcgagccgt cccttgctca cgaggaagct ccggctggcg
     2341 ccctcacggg tccacgagac cagctcgttg cccgtcatgc gacaccacgc cggcagatcc
     2401 tggtccacgg aggtctccgt cgagcggatc tcgaggaggc cgccgcgcgg cagcgggtcc
     2461 atgtgccggc ggatcagcag caggagcccg ccgccgcagt cgaggtcgcc gccgtcgaag
     2521 ctcgcgtcgg gcgcaaacgg atggcgcgcc acagggtcca tcactcctcc acgcagctgc
     2581 ccggatatag aggagcgcca cggcttcgcc ggggcgctcc gaacgttggg ggattggggg
     2641 ccatttcggg gcccccatgt caatagctga cgcaggggct cccgtccgac aggaactcca
     2701 cgagcttggc gccgccgacg acggccgcgc cggcgacgag actgtgctcg tcgagcttgc
     2761 gcttcttgaa gcagggcgcg cagacgtaca tcgtgccgcc acccttcgcg aagttgtcca
     2821 tcagctcgcg caggggcgcg aagccttcct cgtggatcgc gtccgcgtag cccttctggg
     2881 agaggcgcac gccctcgacg ctcaggaaga ccagcgtctc cttgccggat ccgagcgcgg
     2941 cgttggcgac gacgaaggcg accgtggcct tgtcgctgtt gtccttggcg cacgtgagac
     3001 tgacgcagaa cttgttggcc atgactcagc tctcctttcg gcggatccgg tagacagggg
     3061 gtctctgctc caccagcgcg tgcccggtga gccggcacca cgccgggagg tcggccgggg
     3121 ccccggggtc gcgcgcgacc agctccagca cctgtccagg ccg
//
LOCUS       DATIAZ010000123        19576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1214254, whole genome shotgun sequence.
ACCESSION   DATIAZ010000123 DATIAZ010000000
VERSION     DATIAZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 19576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19576
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214254"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VLF19_04145"
     CDS             <1..665
                     /locus_tag="VLF19_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HSE92475.1"
                     /translation="HFVNVTDHRATVAPFRGTDARFVTNPVCIALPGTDRQPPLLLDM
                     ATSAVAMGKVRVARNEGHLVPEGILIDEAGRPTRDPNVMYREPHGALLPFGGHKGYAL
                     AVVTELLAGALSGGPTIQPGNPRLGGVVNNMTTLLIDPARLAGNAWLHREIDGFVDYV
                     KASPPTDPSAPVLVPGDPERIARAERARTGVPVDATTWQEILEAGEKVGLARAQAEAL
                     VA"
     gene            complement(669..821)
                     /locus_tag="VLF19_04150"
     CDS             complement(669..821)
                     /locus_tag="VLF19_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92476.1"
                     /translation="MSLTQTALAGVLQAELEPSEATAASVAWNKLLLVNLTVQLLGYL
                     PPRRLP"
     gene            922..1083
                     /locus_tag="VLF19_04155"
     CDS             922..1083
                     /locus_tag="VLF19_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92477.1"
                     /translation="MPAGWSERLCRQEFARGRRDAQYKWSAMPAKWDDASRAEVVHRR
                     DAVEHPDGL"
     gene            1096..2307
                     /locus_tag="VLF19_04160"
     CDS             1096..2307
                     /locus_tag="VLF19_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92478.1"
                     /translation="MDFAFTTEEQAFAVEVRRFLAEHPPERFPVDGMDAGYGSGSHSR
                     AFLRALAQQGWLAMTWPKEHGGQERPMFFKLVLLEELALAGAPFGPLAGCWQTADAII
                     EYGSDRLRHEVLPATARGEATFWQGYSEPGAGSDLLALTTEARRDGDDYVIRGHKIWS
                     SHAGISSYGLVFARTSREARRSRGFSMFVVPNGTAGMDIRPIRSLTGDVYHYEVFLDD
                     VRVPADMRLGPEGEGFVALLNGLDSDRFWGRFYKAPAIERVLRQLVDYANTTPVRGTS
                     LARDPAVRRRLADAAVDVAALRTLFYRAACLLRDGVPITYETAVAKILADETGQKVAR
                     LGMDLLGLWGPLRAGSRSARLDGQIAHLYQTSLGHTIAGGTAEVLRTTVAVRGLGLPA
                     ETKAADGRGRS"
     gene            2369..3478
                     /locus_tag="VLF19_04165"
     CDS             2369..3478
                     /locus_tag="VLF19_04165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92479.1"
                     /translation="MDFAPSAAQQMLASTAREFLRRHCPPERVQAWALDERGFDEPLW
                     RRMAELGWPGLLVEPALGGSGGSMLDVVLLVEEMGYACLPGPFVQSAVVATSLLAAAG
                     SAEQQKRLLPALAAGERIATVALTEESGSGDPDDVALGCRLPGRVTGRKLFVKDAHVA
                     DVLVVVVREGEGLSLALLPTDRPGISRLPLDPISGEKTFEVVFEDVEVVADDRLGPAG
                     LGGAFLAPALRAGALARTAEMVGGAQRILDLVVEHARTRVQGGRPIGGYQAIQHACAD
                     LVRDVDTTRGLLRLAAWETAEGLPAASDVAMAKAHAAEACLAVARRGHQIFGAIGYCE
                     EHLLHLLHKRIHAAALDFGDRQHHLETVARSMGLK"
     gene            3681..8480
                     /locus_tag="VLF19_04170"
     CDS             3681..8480
                     /locus_tag="VLF19_04170"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HSE92480.1"
                     /translation="MNRRLALRLIATGAVLVALVATVLIALPGIVRWLAVSRLAAATG
                     RAVTLDAVDVALFQGRLGLRGLRVIDRDGAPLLTVHAAAVRFRPRELARGHLRIVDAT
                     VQAVALRIVRTGPDEFNISDLLTPRAARGGFMPAITIERFALTGAVVVEDRTLTPART
                     WRLEAVELNARDASTVAGSPPGGATLSAVAAGAPLSLSMTELRLAPLHFRATLNAREI
                     DASLAALYLPPASALSPKRGKVSVAAAIDHDAATGTLVALDAGFADVELYRSGQQSAY
                     LSAPAVRVTVQGLRVGPGGVELARLAVNGGTITLEDARLASARRWQVDGVALEARDLS
                     SAREAPPGVATARAATTGARVEVWGTNVRLAPFELQATVIVRNVDLSLFRLSLPPELP
                     IHPERGVVNATVRIEHGRRGTRLGLDAGLSNIELQRPAHFVTAPSVRVTADDITLDRG
                     AVSVGRVDVVGGRLTLEERTARPVRTWPVQNLVLEAKDLSSRRDAVQGVASLRATVAG
                     ATAAVFVTGARLDPLELRATTILRDIDAALVRLYVPPEMPVQLARGVVNATFEVDYAV
                     ASGTTLTGDATLTGLEARGRAALATLGVTAPSLRVTVADGRHHGESLSVGRVELSGSG
                     SLTDSRGTSARFDLAQMRIATEGLTWPVTAPARVEVSMRFRDRGELDGSGTARLTAPL
                     PTIAWAAELGLQLKRVDLTPLAVYVPAASGLAGRVRADVTASLAYAGSLTARVRGDVG
                     GARFALAEGGRTLLSLRSIRATGLDLQWPEHMVIKQLRLRQPYALVTRDPRGAFPLAA
                     RFAAPPPEGRASPPAAPEPAGAAPGPAEGSGSPALPTIEITEAIVENGSAAFVDESTI
                     PPLRIDMPRVDLTTRNVTWPASAPIDLVLTGTFPEGGTVKAEGTATVAPASVDVRVAF
                     QDAELAALQPYMGFQARIGGRLNATLAVAGPLTPAPRLKISGDAGLRRFSISDGARSV
                     ITADGLRVTGIDADWPTRIAFDRIGVRRSWALLERDSQGRFLLRTLLGRAAGTGVLRP
                     AGSEVPAAAAPPPVAPAPAPTAGSALELSFREGVFAEQSATIVDSMTTPPARIDVADA
                     RLTVHDFTWPSRAPAKVELTSPMPGGGRLEATGTVDLEPLRLEGRTVLDGVAIEPAQP
                     YLPIEGRVAGKVTGDLTVKLALEPVAVQVAGQARLQAFRLNDGERTVMTVGRVDTAGV
                     DVDWPARISLERVQFRRPRLLIERDADGQIRLRRLVTPHWATTAPATSVPTRSTPAPS
                     TPPGRPPAIEIGTFSLERASARFVDQTTTPAYAEELEDLNVTFTPLTTVPGRRTRVTA
                     SGVIGGGSFKLHGDGAYGERSALDLKTELRKFVVPRANPYLDRYTAWIATNGSLDLTS
                     TYTVDGTRLDTRHDLVVRGLEVAPTDARDEVERRVGMPFGLLVSLLKDSRGEIRLSLP
                     VSGDLSTREFDYREAVWGAVRNLTIRLLALPFSKIGSLSFSPDSRVKAVALAPVVFEA
                     GTARLGPGMEPHLARVADFLRDAPAMKVVLEPVLVEDDVQALKRATAEALPADALRAL
                     AMRRLEVVRQGLTGGGGIDAARFGGMARRTPLVEAAGSGRVEFDLRP"
     gene            complement(8541..9857)
                     /locus_tag="VLF19_04175"
     CDS             complement(8541..9857)
                     /locus_tag="VLF19_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92481.1"
                     /translation="MNLDLLPTTSFTSLGYGTTTWNALAASALAEWNAVGIGNPPDHQ
                     FFSVRVPTVTGDPCSRDGINEVRFASNVCGLSWGDSIGVTQTWISNGKVVEADVLFRT
                     DIRLNAYPGPVVSDTGETLFDFYRLTLHEFGHAAGLIHPNLDGGQNVVALMNSGNQVV
                     GGAQSAIDHLQPDDVAGARSIAWSGSPLAAAVLPSSRSVLVGGAPATAFATIINLGRI
                     TATQCSVSPTTNLPAAFIYQTTNPATNQLTGTANTPVDIPAGVPQSFVFGFSPGAAIA
                     PTDVVLSFTCANAGPAPSTRGLNTLLLSASTTPIPDIVALAATSTNDGIVAIPGATGT
                     GAFAVATVNVGAGGTITASADTGGVALPITLALCQTDPGTGACLGGPVPAVTTTIDAS
                     ATPTFAVFVTGRGSVVFAPATSRIYVRFKDAGGATRGATSVAVRTQ"
     gene            complement(9998..10591)
                     /locus_tag="VLF19_04180"
     CDS             complement(9998..10591)
                     /locus_tag="VLF19_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92482.1"
                     /translation="MSLRPAARWARAFGLVVVALLSAPNGPALAHSFLSKNFDQLVVE
                     AEEIFVGTLTAASSSRLATGTIVTDLTFSGVQVVKGHSSSDVRTVRILGGTVGDLTLK
                     IMGTPDFDRGRTYLLFSKENGRAILPFVGGAQGVFRVVPDATTGTELIVDARGHPMVG
                     SFGGAPGRGVAASRDGSGRPVVSLDVFIQAIRDRLPR"
     gene            10902..11069
                     /locus_tag="VLF19_04185"
     CDS             10902..11069
                     /locus_tag="VLF19_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92483.1"
                     /translation="MTGCLDGVRMLELSKTPARVGPVPVPGQHTDEVLGCLLDYARAT
                     LDALRNAGAIA"
     gene            11267..12505
                     /locus_tag="VLF19_04190"
     CDS             11267..12505
                     /locus_tag="VLF19_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92484.1"
                     /translation="MDPQEVHRNLTRSVVATGEPSWPTRNVLFEHGLFEGFAERPETV
                     IADLHRAMVAAGGDLDLLFGLAELSFLHGGASDKREYQLAAAVYAFAFLFPEDGGPAP
                     GRFDPRLRVAADLYNLALTEGFASEDRSEVVPRAGTFELPFGRLEVAFDQAALRAGDR
                     ELYRFIPVAELDVRGLAMRYRWAGLGAPLAASARPIDASKPGRDLIAPRIQVPVTALL
                     RVPQARRALVQGQPLGGTLELHLAWDAETVSIAGERVPLENEPTAALALALTDAPVVE
                     VELFGFLGRLSGLLRQRPPLVSTTPYKPGLIPVVFVHGTASSIVRWAEMFNRLQADPE
                     IRSRYQFWFFQYDSGNPVALSSLHLREALKGAVARLDPEGKDPALRRMVLIGPRDHRG
                     SAAHPPAARGREVTTRHVVS"
     gene            12502..14625
                     /locus_tag="VLF19_04195"
     CDS             12502..14625
                     /locus_tag="VLF19_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92485.1"
                     /translation="MRRAPAGGGLRGGFTVGILLLLFTLPSGTLADETAAPRTVLALF
                     EETRLLPSVAETDDAIRSTLQAGSPTPIRLYTEYLGLSWFPEEGRELALRDLLQKTYA
                     SRKPDVVIVWGTGALRFVLRHRAALFDGVPVVFCAVDPALLPRLDAGSAVTGVSRRID
                     WTDALDLIMRLHPGTREVAIVTGSGAVDLRWEAEARRAFHGYRGEARLTYLARRPMPQ
                     LLQAVGSLSPGTIVLYGAVLQDGAGRPYLPREALALVVEASRVPVYAVTANMLGHGVV
                     GGRLLDFPSQGVTAAKLALRVLGGERLGPGDIVEADNPYLFDWRQLRRWQIDEGRLPP
                     ASVVRFRQASVWDLYKDYIIAAVILIAAQSLLIAALLVQRARRQRAEDEARARREELA
                     HAQRVATIGELSASLAHELNQPLTAIATNAQAARRSMATSRAEGGELSEVLVDIADDA
                     RRAGAIVRRMRALVRKQPGERQPVDVNLAVTEVTRILRRDIARAEGGELSETLVDIAD
                     DARRAGAIVRRMRALIRKQPGERQPVDINLAVTEVTRILRRDIAQAEVSLHLRLGEDL
                     PPVIGDVVQLQQVVLNLLINACQAMAGVENRPRVLRIETSEPKPGSIEIAVADAGAGV
                     PESELERMFEPFVSSKAGGLGMGLSISRSIIEAHDGHIWATRNPDRGLTLHVTLPSAS
                     TGHANPRYPVGPPGPDEVPPVAAGR"
     gene            14570..14830
                     /locus_tag="VLF19_04200"
     CDS             14570..14830
                     /locus_tag="VLF19_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HSE92486.1"
                     /translation="MPWALRVPTRSRRSRQGDDTDDDGCACRKPKAGMFLELATTCGL
                     DLADSMHVGDSAKDRAAARAAGVGRFVPAREFFGWSDAPNAR"
     gene            complement(14827..15240)
                     /locus_tag="VLF19_04205"
     CDS             complement(14827..15240)
                     /locus_tag="VLF19_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92487.1"
                     /translation="MSERIDLVDYFYVEAADKPGEGARMLDHLRGAGVNLLAFSGFPR
                     GRRAQMDFVAADAGRLRAAAKAARWKLVGPKKAFLVQGDDRVGAVSEIFDKLATEKIN
                     VTAADAVCAGSGRYGMIFWVKPGDVKRAAKALGVG"
     gene            complement(15355..17163)
                     /locus_tag="VLF19_04210"
     CDS             complement(15355..17163)
                     /locus_tag="VLF19_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92488.1"
                     /translation="MRQPLVHLLRRSIALKLTLTLVGFVAVAVTFAGLYLDQALETFA
                     VEALESRLTTAGALLHDEARGLLAGPASAPAVRAFAVRAAEPSRARVTLVAADGRVLG
                     DSEVPVADLQRVESHGDRPEVRAALDGRIGRHVRRSATVNAPLLYVALPVAEGGRVLG
                     VLRFAFPLSLVTDSYAVVHRAMLAGTAVALVVALGIGLFVARRVTKPVVDMHAIARRM
                     SEGDFTARASVRSPDEIGQLGRALNVMSARLREKLGDLEHEQGKVAAILDGMVEGVIA
                     VDGRDHVVLMNERARAICGLGTARAERKPFLEVIRNAELHQLLRASRAAGEGVVSRRE
                     LTLAGPVDRTLQATAVPLQLGADEVGALMVLHDVTELRRLEQVRTEFVANVSHELRTP
                     LTAIRGYLETLLGGALEEPAHARRFLEIAARHTERLGRLLDDLTDLSNIELGRVTLRL
                     EPTALGDTVESVLAIVRPRAEAGGVVLAAAVPPDLPPVHADHDRLVQVLLNLVDNAVK
                     YTPQGGSVTVTAHAGGEPGVEITVRDTGVGIPASDLPRITERFYRVDRARSRELGGTG
                     LGLAIVKHLVLAHGGGLAITSEVGQGTTVRVTLPVA"
     gene            complement(17163..17849)
                     /locus_tag="VLF19_04215"
     CDS             complement(17163..17849)
                     /locus_tag="VLF19_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSE92489.1"
                     /translation="MAQEVLVVEDEPDIRNLIVFHLGREGYRCRTASTGPQALAEARA
                     SRPDLVVLDLMLPEMDGLDVCRRLRGDPRTAQLPIIMLTAKADEVDRVVGLEIGADDY
                     VAKPFSPKELVARVRAVLRRSRSGDDAAALRAGHVTVDPARHAVEVGGVRVELTPKEF
                     DLLHALMEASGRVLSREYLLDHVWGYARASEVESRTVDVHVRRLRAKLGDEGRRIATV
                     KGVGYRFDGD"
     gene            complement(17981..18961)
                     /locus_tag="VLF19_04220"
     CDS             complement(17981..18961)
                     /locus_tag="VLF19_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSE92490.1"
                     /translation="MEWFVVVLLLVLAAEFVNGWTDAPNSIATVVSTRVLGPFQALAM
                     ASVLNVLGAMSGTAVAATIGKGMVKPEAINVATVAAAMIAIVLWSTLAWSYGLPTSES
                     HALIAALSGAGLATAGPSVLLWDGWQKVLLGLVFSTFLGFLAGLGLTIGIYWIFRRWR
                     PGTVRALFGRLQILSAAFMAFSHGSNDGQKFIGAFTLTLVLGGVLPTFAVPLWVILLC
                     GATMGVGTAIGGWRIVKTMGLGLTKLDPVHGFAAETAAATTIELATRLGVPLSTTHTI
                     NTAIMGVGSTRRVSAVRWGVGGEIVTAWILTFPVCGLISWAVAKLLAALR"
     gene            complement(18969..>19576)
                     /locus_tag="VLF19_04225"
     CDS             complement(18969..>19576)
                     /locus_tag="VLF19_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSE92491.1"
                     /translation="IPREVRFFDYFEQQSQHILRAAELLHELVHHFADARAKMHAIKE
                     IEHQGDQVTHEVVKRLHQTFITPLDREDIHALASRLDDVLDYIEASAERLLIYRIKEP
                     TSACRALTDVIVQITRAVDHTVRCLRTRDPRFHEHAVEVNRLENSADNLLRDSLAELF
                     EQQGDPIEIIKWKEIYETLEVVTDRCEDVANVIEGIILKMA"
BASE COUNT         2731 a         7100 c         6975 g         2770 t
ORIGIN      
        1 tgcacttcgt caacgtcacc gatcaccggg cgaccgtcgc gcccttccgc ggcacggacg
       61 cgcgcttcgt caccaatccc gtgtgcatcg cgctgcccgg caccgaccgg cagccgccgc
      121 tgctgctcga catggcgacc agcgcggtcg ccatgggcaa ggtgcgcgtc gccaggaacg
      181 aaggccacct ggtgcccgag ggcatcctga tcgacgaggc cgggcgcccc acgcgcgatc
      241 ccaacgtgat gtaccgcgag ccgcacgggg ccctgctgcc tttcggcggg cacaagggct
      301 acgcgctggc ggtggtgacc gagctgctgg cgggcgccct gtcgggcggg cccacgatcc
      361 agcccggtaa cccgcggctg ggcggcgtgg tcaacaacat gacaacgctc ctgatcgatc
      421 ccgcccgcct ggccggcaac gcgtggctcc atcgcgagat cgacggcttc gtggactacg
      481 tgaaggcgtc gccgccgacc gatccgtcgg cgccggtcct ggtccccggc gatccggagc
      541 ggatcgcccg cgcggagcgc gcgcggaccg gggtccccgt ggacgcgacg acgtggcagg
      601 agatcctcga ggccggcgag aaggtcgggc tcgcccgcgc gcaggccgag gcgctcgtcg
      661 cgtgacggtc acggaagccg ccgcggcggc aggtagccga gcaactgcac ggtcaggttg
      721 acgagcagca gcttgttcca ggcgacgctg gccgccgtcg cctcagacgg ctcgagctcg
      781 gcttgcagca cgcccgccag cgccgtctgc gtcaggctca tcgcgccgac catcaggtgc
      841 ggcgggaccg tcccgcccgc aacgtcgagt acgcgtgcaa gcgccgctcg gcccgccttg
      901 tgccccccgc ggctgtgcgt catgccggcg ggctggtccg agcggctgtg ccggcaggag
      961 ttcgcgcgcg gccggcgcga cgcccagtac aagtggagcg ccatgccggc gaaatgggat
     1021 gacgccagcc gcgccgaggt cgtccatcgc cgggacgcgg tcgagcaccc ggatgggcta
     1081 tagtccgact ggtccatgga cttcgcgttc accaccgaag aacaggcctt cgccgtcgag
     1141 gtcaggcgct tcctcgccga gcatcccccg gagcgcttcc ccgtcgacgg gatggacgcc
     1201 ggctacggct cgggctcgca ctcccgcgcc tttctccggg cgctcgccca gcagggctgg
     1261 ctcgccatga cgtggccgaa ggagcacggt gggcaggagc ggccgatgtt cttcaagctc
     1321 gtcctgctgg aggagctggc gctcgccggc gcgccgttcg gcccgctggc cggctgctgg
     1381 cagaccgccg acgcgatcat cgagtacggc agcgaccgcc tgcgccacga ggtgctgccg
     1441 gcgacggccc gcggcgaggc gaccttctgg cagggctaca gcgagcccgg cgccggctct
     1501 gacctgctcg cgctgacgac ggaggcgcgg cgggacggcg acgactacgt catccgcggc
     1561 cacaagatct ggtcgagcca cgccggcatc tcgagctacg gcctggtgtt cgcgcggacc
     1621 agccgcgagg cgcgtcgcag ccgcggcttc agcatgttcg tggtgccgaa cggcaccgcc
     1681 ggcatggaca tccggccgat ccgcagcctc accggggacg tgtaccacta cgaggtgttc
     1741 ctggacgacg tgcgcgtgcc ggcggacatg cggctcggac ccgagggcga gggcttcgtg
     1801 gcgttgctga acggcctcga ctccgaccgc ttctggggcc gcttctacaa ggccccggcc
     1861 atcgagcgag tgctgcgcca gctcgtggac tatgcgaaca cgaccccggt gcggggcacg
     1921 tcgctcgcgc gtgatcccgc cgtgcgccgg cggctggccg acgccgccgt cgatgtcgcc
     1981 gcgctgcgca cgctcttcta ccgcgccgcc tgtctcctgc gcgacggcgt gcccatcacc
     2041 tacgagacgg cggtggccaa gatcctcgcc gacgagacgg gccagaaggt cgcccggctc
     2101 ggcatggacc tgctggggct gtggggcccg ctccgcgccg gctcgcggtc ggcgaggctc
     2161 gacgggcaga tcgcgcacct ctatcagacg agcctcggcc acaccatcgc cggcggcacc
     2221 gccgaggtgc tccggaccac cgtcgcggtg cgtggtctcg gcttgccggc cgagacgaag
     2281 gccgccgacg gccgcggccg gagctgaccg gatggacttc gcgccgttca tggcccggcc
     2341 aatgctccac tgtgtcgccc gctgaacgat ggacttcgcg ccgtcggccg cccagcagat
     2401 gctcgcctcc accgcccgcg agttcctgcg gcgccactgc ccccccgagc gggtgcaggc
     2461 gtgggcgctc gacgagcgcg gcttcgacga gccactctgg cgccgcatgg ccgagctcgg
     2521 ctggcccggg ctgctggtgg agccggcgct cggcggcagc gggggctcga tgctcgacgt
     2581 ggtcctgctc gtcgaggaga tgggctatgc ctgcctgccc ggcccgttcg tccagagcgc
     2641 ggtggtcgcc acgtcgttgc tggccgccgc gggcagcgcg gagcagcaga agcgcctgct
     2701 tcccgccctg gcggccggcg agcgtatcgc cacggtggcg ctgacggaag agagcggctc
     2761 gggcgatccc gacgacgtcg cgctcggctg ccggctgccc gggcgcgtga ccgggcgcaa
     2821 gctcttcgtc aaggacgctc acgtcgccga cgtcctggtg gtcgtcgtgc gcgagggcga
     2881 gggcctgagc ctggcgctgc tgcccaccga ccggcccggc atcagccgcc tccctctcga
     2941 ccccatcagc ggcgagaaga ccttcgaggt ggtcttcgag gacgtggagg tcgtggccga
     3001 cgatcgtctg ggaccggcgg gcctcggcgg cgcgttcctg gcgccggcgc tccgggccgg
     3061 cgcgctggcg cgcaccgcgg agatggtggg cggcgctcag cgcatcctcg atctcgtcgt
     3121 cgagcacgcc cggacccgcg tgcagggcgg gcggccgatc gggggctatc aagcgatcca
     3181 gcacgcgtgc gcggacctcg tgcgggacgt cgacaccact agggggctgc tccgtctcgc
     3241 ggcgtgggag accgccgagg gtctgcccgc cgcgagcgac gtcgcgatgg ccaaggccca
     3301 cgccgccgag gcgtgcctgg cggtcgcgcg acgcgggcac cagatcttcg gcgccatcgg
     3361 ctactgcgag gagcacctac tgcacctgct gcacaagcgc atccacgccg cggcactcga
     3421 cttcggcgac cggcagcacc acctggagac cgtcgcccgt tccatgggcc tgaagtaaga
     3481 gcgcgcgacg cccgcgagac tcgcacagca ccgggaggcg cccatggcgg agaaaccggc
     3541 ggtcagcctg gtcgcggtgg cgggaggccg ccgcgccacc ctggagctgg gcccagcgta
     3601 tcgagcgcaa gggattctcg ggggccgact tccggtgaac gccgtacgat ggtgccgtcc
     3661 gtggttatca tgaagacctg gtgaaccgtc gcctcgcgct tcggctcatc gccaccggcg
     3721 ccgtgctcgt cgcgctggtg gcgacggtcc tgatcgccct gcccgggatc gttcgctggc
     3781 tggcggtctc gcggctggcg gcggcgacgg ggcgggcggt gaccctcgac gccgtcgacg
     3841 tcgcgctgtt ccagggccgg ctcggcctcc gcggcctccg ggtgatcgac cgtgacgggg
     3901 caccgttgtt gaccgtccac gcggccgccg ttcgattccg cccgagggag ctggcccgcg
     3961 gccacctgcg catcgtggac gcgaccgtcc aggcggtggc cctccgcatc gtgcggaccg
     4021 ggccggacga gttcaacatc tccgatctcc tgacaccccg cgccgcacgt ggcggcttca
     4081 tgcccgccat cacgatcgag cggttcgccc tcaccggagc cgtcgtcgtc gaggaccgca
     4141 cgctgacgcc ggcacgcacg tggcgcctgg aggcggtgga gctgaacgcc cgggacgcgt
     4201 cgacggtggc cgggagtccg ccgggcggcg ccacgctgag tgccgtggcg gccggggcgc
     4261 cgctctccct gtcgatgacc gagctgcgcc tcgctccact ccacttccgc gccacgctga
     4321 acgcgcggga gatcgacgcc tcgctcgccg ccctctacct gccgcccgcc agcgcgctca
     4381 gcccaaagcg cggaaaggtc agcgtggcgg cggcgatcga ccacgacgcc gcgacgggta
     4441 cgctcgtcgc gctcgatgcc gggttcgccg acgtcgagct gtaccggtcg ggacagcaga
     4501 gcgcgtacct gtccgcgccg gcggtgcggg tgacggtgca agggcttcgc gtggggccgg
     4561 gcggagtcga gctggcccgc ctcgccgtga acggggggac gatcacactg gaagacgcac
     4621 ggctcgcttc cgcccggcgc tggcaggtcg acggcgtcgc gctcgaggcg cgtgacctgt
     4681 cgagcgcccg tgaggcgccg cccggggtcg cgacggcccg cgcggcgacg acgggcgcgc
     4741 gcgtcgaggt gtggggtacg aacgtccgcc tggcgccgtt cgagctgcag gcgaccgtga
     4801 tcgtccgcaa cgtcgacctt tcgctatttc gcctctccct gccgcccgag ctgccgatcc
     4861 accccgagcg cggtgtcgtc aacgccaccg tccggatcga gcacgggcgc cgcggcacgc
     4921 gcctgggctt ggatgccggg ctgagcaaca tcgagctgca acgccccgcc cacttcgtga
     4981 ccgcgccctc ggtgcgggtg accgccgacg acatcacgct cgaccgcggg gccgtgagcg
     5041 tcggccgtgt cgacgtcgtc ggaggccgcc tgacgctcga ggagcggaca gccaggccgg
     5101 tgcggacctg gccggtccag aacctggtcc tcgaggccaa ggacctgtcc agccgccgcg
     5161 atgccgtcca gggcgtcgcc agcctccgag cgaccgtcgc cggcgccacg gcggcggtgt
     5221 tcgtcacggg cgcgaggctc gaccccctcg agctccgcgc gacgacgatc ctgagagaca
     5281 tcgatgcggc cctggtccgc ctgtacgtgc cgccggagat gccggtgcag ctcgcccgcg
     5341 gcgtcgtcaa cgccaccttc gaggtggatt acgccgtcgc cagcggcacg accctgacgg
     5401 gcgacgcgac gctcaccggt ctcgaggcgc ggggccgcgc tgcgctggcg accctggggg
     5461 tcacggcgcc ctcgctgcgg gtgacggtcg ccgatgggcg gcatcacggc gagagcctga
     5521 gcgtcggccg ggtcgagctg tccgggagcg gctcgctcac cgactcgcgc gggacgtccg
     5581 ctcggttcga tctcgcgcag atgcgcatcg ccaccgaggg cctcacgtgg cccgtcacgg
     5641 cgcccgcgcg cgtcgaggtc agcatgcgct tccgcgaccg tggcgagctc gacgggagcg
     5701 gaacggcacg gctcaccgct ccgcttccca cgatcgcctg ggcggccgag ctgggtttgc
     5761 agctcaagcg cgtggacctc acgcccctcg ccgtgtacgt gccggccgcg agcggactcg
     5821 ccgggcgcgt gcgagccgac gtcacggcga gcctcgcgta tgccggctcg ctgacggcgc
     5881 gggtccgcgg cgatgtgggc ggggctcgct tcgctctcgc cgagggcggc cggacgctgc
     5941 tgtccctccg gagcatccgt gcgaccggcc tggatctgca atggccggag cacatggtca
     6001 tcaagcagct ccgcctgcgt cagccatacg cgctcgtcac gcgtgacccc cggggcgcgt
     6061 tcccactggc cgcccggttc gccgcgccgc cgcccgaggg cagggcgtca ccacccgcgg
     6121 cgccggagcc ggcgggtgcg gcgccggggc cggccgaagg ctccgggagc ccggcactcc
     6181 cgacgatcga gatcaccgag gccatcgtcg agaacgggag cgctgccttc gtggacgaga
     6241 gcaccatccc gcccctgcgc atcgacatgc cccgggtgga tctcacgacc cgcaacgtga
     6301 cctggcccgc gtcggccccc atcgacctcg tgctgaccgg gacgtttccc gagggcggga
     6361 cggtaaaggc cgagggcacg gccaccgtcg cgcccgcgag cgtcgacgtc agggttgcgt
     6421 tccaggacgc cgagctcgcc gcgctccaac cgtacatggg cttccaggca cgcatcggcg
     6481 gccggttgaa tgcgacgctg gcggtggccg gtccactgac gccggcgccg aggctgaaga
     6541 tcagcggcga tgccgggctt cgcaggttct cgatctccga cggggcgcgg tccgtgatca
     6601 cggccgatgg tctccgcgtg acgggaatcg acgcggattg gccgacgagg atcgcgttcg
     6661 accgcatcgg cgtgcggcgc tcctgggcgc tcctcgagcg cgactcgcag ggccgcttcc
     6721 ttctgcgcac gctgctcggc cgcgccgccg ggaccggcgt cctgcgcccg gccggctccg
     6781 aggtgcccgc tgccgcggca ccacctccgg tggcacccgc gccggctccc accgccggct
     6841 ccgcgctcga gctcagcttc cgggagggag tcttcgccga gcaatcggca accatcgtcg
     6901 acagcatgac cacgccgccg gcccgtattg atgtcgccga cgcccgcctc accgtccacg
     6961 acttcacgtg gccgtcccgg gcccctgcga aggtcgagct gacctcgccg atgccgggag
     7021 gcggccgcct cgaggccacg ggcacggtcg atctcgagcc gctgcgcctg gagggccgca
     7081 cggtgctgga cggcgtggcc atcgagcctg cgcagccgta tctaccgatc gagggccggg
     7141 tggcagggaa ggtcaccggc gatctgacgg tgaagctcgc cctcgagccg gtggctgtcc
     7201 aggtcgcggg ccaggcgcgc ctgcaggcgt tcaggctgaa cgacggggaa cgcacggtga
     7261 tgacggtggg ccgcgtcgac accgccgggg tcgacgtcga ctggcccgcg cggatcagcc
     7321 tggagcgcgt gcagttccgc cggccgcggc tgctgatcga gcgcgacgcc gacggtcaga
     7381 tccggctgcg ccggctggtg acgccgcact gggcgaccac cgcgccggcc acatcggtcc
     7441 cgacccgatc gacgccggca ccatcgacgc cgccgggccg gccaccggcg atcgagatcg
     7501 ggaccttcag cctcgagcgg gcctcggcgc gcttcgtgga tcagacgacg acgccagcct
     7561 acgccgagga gctggaggat ctgaacgtga ccttcacccc gttgacgacg gtgcccggcc
     7621 gtcgcacacg cgtcacggcc agcggcgtca tcggcggcgg ctcgttcaag ctccacggcg
     7681 acggggcgta cggggagcgc tcggctctcg atctcaagac ggagctccgg aaattcgtcg
     7741 tgcctcgcgc caacccctat ctcgaccggt acaccgcctg gatcgcgacg aacgggagtc
     7801 tcgacctcac gagcacctac acggtcgacg ggacgcggct cgacacccgc cacgatctcg
     7861 tggttcgcgg cctcgaggtc gcccccaccg atgcgcgtga cgaggtcgag cggcgtgtcg
     7921 ggatgccctt cggcctcctg gtctcgttgc tgaaggactc ccgcggcgag atccggctct
     7981 ccctgcccgt ttccggcgac ctcagcacgc gggagttcga ctatcgcgag gcggtctggg
     8041 gggccgttcg taacctcacc atccgactcc tggccctgcc gttcagcaag atcggctcgc
     8101 tctccttcag tccggattcc agggtcaagg ccgtggcgct ggcgcccgtc gtcttcgagg
     8161 cgggaacggc tcgcctggga ccggggatgg agccccacct cgcgcgtgtc gccgacttcc
     8221 tgcgcgacgc gccggcgatg aaggtggtgc tcgagccggt cctcgtcgag gacgacgtcc
     8281 aggcgctcaa gcgggcgacg gcggaggcgc tgccggcgga cgcgctgagg gcgctcgcca
     8341 tgcgccgcct cgaggtcgtg cgtcaggggc tcacgggcgg cggcgggatc gacgcggcgc
     8401 gcttcggtgg aatggcccgg cgcacgccgc tcgtcgaggc cgccggcagc ggacgggtgg
     8461 agttcgatct gagaccgtga agccgtggat tcgcggacgc gctcgccgga gggcgcggcg
     8521 accgcgagat catcgtgacg tcactgggtc ctcaccgcga cgctcgtcgc ccctcgagtc
     8581 gcccctcccg catccttgaa gcggacgtag atgcgactgg tcgccggggc gaagacgacg
     8641 ctgccacgcc cggtgacgaa caccgcgaag gtcggcgtgg cgctggcgtc gatggtcgtc
     8701 gtcaccgcgg gcaccgggcc gcccagacac gccccggtac ccggatcggt ctgacagagc
     8761 gcgagcgtga tcgggagcgc gacgccgccc gtgtccgccg acgcggtgat cgtcccgccg
     8821 gctcccacgt tgaccgtggc gaccgcgaac gcgccggtgc cggtggcccc ggggatggcg
     8881 acgatcccat cgttggtcga ggtggctgcc agcgccacga tgtccgggat cggcgtcgtc
     8941 gaggccgaca ggaggagcgt gttcaggcca cgggtgctcg gcgccggccc cgcgttcgcg
     9001 cacgtgaagc tcagcacgac gtcggtgggg gcgatcgccg ccccggggct gaagccgaac
     9061 acgaagctct ggggcactcc ggccggaatg tccaccggcg tgttcgccgt tcccgtgagc
     9121 tggttggtgg caggattcgt cgtctgatag atgaacgcgg ccggaaggtt cgtcgtgggc
     9181 gagacggaac actgggtcgc cgtgatgcgg cccaggttga tgatcgtcgc gaaggctgtc
     9241 gcgggcgctc cacccaccag gacggaccgg ctgctcggga gaaccgcggc ggccagcggg
     9301 ctgccgctcc aggcgatgga gcgcgcgccg gcgacgtcgt ccggctgcag gtggtcgatg
     9361 gcgctctggg cccctccgac gacctggttc cccgagttca tgagagccac gacgttctga
     9421 ccgccgtcca ggttcggatg gatcaggccc gcggcgtggc cgaactcgtg caacgtgagc
     9481 cgatagaagt cgaacagcgt ctccccggtg tcgctgacga cgggtccggg ataggcgttc
     9541 aggcgaatgt ccgttctgaa cagcacgtcg gcctcgacga ccttcccgtt gctgatccac
     9601 gtctgggtga cgccgatgga gtctccccac gacaggccgc agacgttcga ggcgaatcga
     9661 acctcgttga tcccgtcccg cgagcacggg tctccggtga ccgtcggcac cctcaccgag
     9721 aagaactgat gatcgggcgg gttcccgatc ccgacggcgt tccactcggc gagcgcgctc
     9781 gcggccagcg cgttccaggt cgtggtgccg tatcccaggc tcgtgaagct cgtggtcggc
     9841 aggagatcga ggttcatcgt cagccgattc tgtggaggct ccgggcggag gacgaatcca
     9901 ccggtcacgc cagcccagga gagtcccagg agcaccgggg ccagcgtcga cagccatctc
     9961 cagagcgtgg acctcccctt caccggccgc ggtgacctca ccgcggcagg cgatcccgga
    10021 tcgcctggat gaacacgtcg aggctcacca cgggtctccc gctgccatct cgcgacgccg
    10081 cgacgccgcg gcctggcgcg cctccgaacg acccgaccat cggatggcca cgggcgtcga
    10141 cgatcagctc ggtgcccgtc gtcgcatccg gaacgacccg gaagacgccc tgcgctccgc
    10201 cgacgaaggg caggatcgcg cgcccgttct ccttcgagaa gagcagatag gtccggcccc
    10261 ggtcgaagtc cggggtcccc atgatcttca gggtgagatc accgacggtg ccgccgagga
    10321 ttcgcacggt gcgcacgtcg ctcgacgagt gacccttgac gacctgcacg ccggagaatg
    10381 tcaggtcggt gacgatggtg ccggtggcga gcctcgacga ggaggccgcg gtgagggtgc
    10441 cgacgaagat ctcctcggcc tcgaccacga gctgatcgaa gttcttcgac aggaacgagt
    10501 gagcgagagc cggcccgttc ggagcggaga ggagcgccac caccacgagc ccgaaggccc
    10561 gagcccaacg cgcagctgga cgcaagctca tcgcgactcc ctcctcatgc tgccagtgcc
    10621 actccggcat cggtcactcc gactcctcgc ggttccacag ccatgagacg gatcctccgg
    10681 ctccgcatcg gcgaggaatc ccggagccgg gtgagctgca gaggttgaaa aattatggct
    10741 ccgtccgttc accatcgtca tcgaaaaagt tgggacgagc cgtgacgcgg gagccgccgc
    10801 ggcgcacctc cgctcctcga gaccaggggc gcccgcgtgg agttccatcc aagcctcgaa
    10861 gaatgtattc ttcgacgcgt tcgaccgatg gaggtgacga gatgaccggc tgcctcgacg
    10921 gcgttcggat gctcgagctc tcgaagacgc ccgcgcgtgt cggcccggtg cccgtacctg
    10981 gccagcacac cgacgaggtc ctcggctgcc tgctcgacta cgcccgcgcg acgctcgacg
    11041 ccctgcggaa cgccggtgcc atcgcgtagc gacgctccgc cgggggtgcg gtcgattcgc
    11101 caacgccagc acgatgaccg gacgacccac gaatcaatcg tcgaggcctt cgcgtacgga
    11161 agcccgtagg gcgcgacgga gccgaatcat ctccagacgg ctgattctcc tccccgccct
    11221 ctgtctggcc ctggcggcgt gcgccggtcc ggtcagggcc ctccgggtgg acccgcagga
    11281 ggtccaccgc aatctcaccc ggagcgtcgt ggcgacaggc gagccgagct ggccgacgcg
    11341 caacgtgctc ttcgagcacg gcctcttcga gggcttcgcc gagcgcccgg agacggtgat
    11401 cgccgatctc caccgggcca tggtcgcggc ggggggcgat ctcgatctgc tcttcggcct
    11461 ggccgagctc tccttcctgc acggcggagc cagcgacaag cgcgagtacc agctggccgc
    11521 ggctgtttac gcctttgcct tcctcttccc cgaagatgga ggaccggccc cggggcgttt
    11581 cgaccctcgc ctcagggtcg cggcggacct gtacaacctc gccctcaccg aaggattcgc
    11641 ctcggaggac cggtcggagg tggttccgcg tgcgggcacg ttcgagctgc ccttcggacg
    11701 cctcgaggtc gccttcgatc aggccgccct tcgggccggg gaccgtgagc tgtaccggtt
    11761 catcccggtc gccgagctgg acgtgcgcgg cctggcgatg cggtatcgct gggcgggcct
    11821 cggcgccccg ctcgccgcct ccgcccgccc catcgacgcc tcgaagccgg ggcgggacct
    11881 catagcgccg cggatccagg tgccggtgac ggccctcctg cgtgtccccc aggcccgccg
    11941 cgcgctcgtg cagggccagc cgctcggcgg gacgctggag ctgcacctgg cctgggacgc
    12001 cgagaccgtc tcgatcgccg gcgagcgggt ccctctcgag aacgagccga cagcggcgct
    12061 ggcgctcgcg ctcaccgacg cgccggtggt ggaggtcgag ctcttcggat tcctcggccg
    12121 gctctccggg ctcctgcggc agcggccccc gctcgtctcc accacgccgt acaagccggg
    12181 cctcatcccg gtggtcttcg tgcacggcac ggcctcgagc attgtccgct gggccgagat
    12241 gttcaaccgg ctccaggccg accccgagat ccgcagtcgc taccagttct ggttcttcca
    12301 gtacgactcc ggcaacccgg tcgcgctgtc atcgctccac ctgcgcgagg cgctgaaggg
    12361 ggcggtggcg cgcctggacc ccgagggcaa ggacccggcg ctgcgccgga tggtgctgat
    12421 cgggccccga gaccatcgag gaagtgcggc gcatcctccg gctgcacgcg ggcgtgaagt
    12481 gaccacgcgg cacgtcgtat cgtgagacga gccccggccg gcggcgggct gcgcggcggc
    12541 ttcaccgtcg gcatcctgct gttgctcttc actctgccgt cgggaacgct cgcggacgag
    12601 accgcggcgc cgaggaccgt gctggcgctc ttcgaggaaa cccggctcct gccctcggtg
    12661 gccgagacgg acgacgccat ccgctcgacg ctccaggcgg gctccccgac gccgatccgg
    12721 ctctacacgg agtacctcgg tctgtcgtgg ttcccggagg aggggcgcga gctcgcgctt
    12781 cgcgaccttc tgcagaagac ctatgcgtcg cgaaagccag acgtggtcat cgtctggggg
    12841 acgggcgcac tccgcttcgt gctcagacac cgtgcggcgc tgttcgacgg cgtcccggtc
    12901 gtcttctgcg cggtcgatcc ggccctcctg ccccggctcg acgctggatc ggcggtgacg
    12961 ggtgtctcca ggcgcatcga ctggaccgac gcgctcgacc tgataatgcg cttgcacccc
    13021 ggaacccggg aagtcgccat cgtgacgggt agcggtgcgg tcgatctcag gtgggaagcc
    13081 gaggcgcgtc gcgcttttca cgggtaccgc ggcgaggcga ggctcaccta cctggcccgc
    13141 cggccgatgc cccagctcct gcaggccgtc ggatccctgt ccccgggcac catcgtcctg
    13201 tatggcgccg ttctccagga cggcgccgga cgcccgtacc tgcctcgcga ggcgctcgcg
    13261 ctggtggtgg aggcttctcg cgtgcccgtc tacgcggtga cggcgaacat gctcggtcac
    13321 ggcgtcgtcg gcggtcgctt gctcgacttc ccgtcccagg gcgtcacggc ggcgaagctg
    13381 gcgctccggg tcctcggcgg cgagcggctc gggcccggcg acatcgtgga ggcggacaat
    13441 ccgtacctgt tcgactggcg ccagctccgc cggtggcaaa tcgacgaggg ccggctgccc
    13501 ccggccagcg tcgtccggtt ccggcaggcg tcggtctggg atctctacaa ggactacatc
    13561 atcgcggcgg tgatcttgat cgcggcgcag agcctcctca tcgcggcgct gctggtgcag
    13621 cgggcgcgac gccagcgtgc ggaggacgag gctcgagcgc gccgcgagga gctggcccac
    13681 gcgcagcgcg tggccaccat cggcgagctc tcggcgtcgc tcgcccacga gctcaaccag
    13741 ccgctgaccg cgatcgccac gaacgcgcag gccgcgcgcc gttcgatggc cacgtcgcgg
    13801 gccgagggtg gcgagctgag cgaggtgctc gtcgacatcg cagacgacgc caggcgggcc
    13861 ggggcgatcg tgcggcggat gcgcgccctg gtccgcaagc agcccggcga gcgccagccc
    13921 gtggacgtca acctggcggt caccgaggtg acgaggatcc tgcgccggga catcgcgcgg
    13981 gccgagggtg gcgagctgag cgagacgctc gtcgacatcg cggacgacgc caggcgggcc
    14041 ggggcgatcg tgcggcgcat gcgcgccctg atccgcaagc agcccggcga gcgccagccc
    14101 gtggacatca acctggcggt caccgaggtg acgaggatcc tgcgccggga catcgcgcag
    14161 gccgaggtgt cgctgcacct gcgcctggga gaagacctgc ctccggtgat cggggacgtc
    14221 gtccagctcc agcaggtcgt gctcaacctg ctcatcaacg cgtgccaggc catggccggc
    14281 gtcgagaacc gaccgcgagt gctgcgcatc gagacgtcgg agccgaagcc cgggagcatc
    14341 gagatcgccg tcgccgacgc cggcgccggc gtgcccgagt cggagctcga gcgcatgttc
    14401 gagcccttcg tctcgagcaa ggccggaggg ctcggcatgg ggctgtcgat cagtcgctcc
    14461 atcatcgagg cgcacgacgg acacatctgg gcgacccgca atcccgaccg cggcctgacc
    14521 ctgcacgtca cgctgcccag cgcgagcacc gggcacgcca acccccgtta tcccgtgggc
    14581 cctccgggtc ccgacgaggt cccgccggtc gcggcagggc gatgacaccg acgacgacgg
    14641 gtgcgcctgt cgcaagccga aggccgggat gttcctcgag ctggccacga catgcggcct
    14701 cgacctcgcc gattcgatgc acgtcggcga ctccgccaag gaccgcgccg ccgcccgcgc
    14761 cgcgggcgtg ggacgcttcg tccccgcccg ggagttcttc ggctggagcg acgcgccgaa
    14821 cgcccgctaa cccacgccta gcgccttcgc cgcgcgcttg acgtcgcccg gcttgaccca
    14881 gaagatcatc ccgtagcgtc cgctcccggc gcagacggcg tcggcggcgg tcacgttgat
    14941 cttctccgtc gcgagcttgt cgaagatctc ggagacagcc cccacgcggt cgtcgccctg
    15001 cacgaggaac gccttcttgg gaccgacgag cttccacctg gcggccttcg ccgcggcgcg
    15061 gagcctgccg gcgtcggcgg ccacgaagtc catctgcgcg cggcgcccgc gcgggaagcc
    15121 ggagaaggcg agcaggttca cgccggcccc gcgcaggtgg tcgagcatcc gcgcgccctc
    15181 cccgggcttg tcggccgcct ccacgtagaa gtagtcgacc aggtcgatgc gctcgctcat
    15241 ggcccctctc cttccatcgg gggggcctcc acggccccct ccgaaacctc ccccagggag
    15301 acgtagcgcc gccgaggccg gcgctcgaac gaggatatcc cgcgcgacgc ccgttcaggc
    15361 gacgggcagc gtcacccgca cggtcgtgcc ctggccgacc tcgctcgtga tggcgagccc
    15421 acctccgtgc gccaggacga ggtgcttgac gatcgccagc ccgagcccgg tgccacccag
    15481 ctcgcgggag cgcgcccggt ccacccggta gaagcgctcg gtgatgcgcg gcaggtcgct
    15541 ggcggggatg ccgacgccgg tgtcgcgcac ggtgatctcg acgccgggct cgccccccgc
    15601 gtgagcggtc acggtgacac tcccaccctg cggagtgtac ttcaccgcgt tgtccaccag
    15661 gttgagcaag acctgcacga ggcggtcgtg gtccgcgtgc accggcggca ggtctggcgg
    15721 gaccgccgcg gccaggacga cgccgccggc ctcggcgcgc ggccgcacga tcgccagcac
    15781 cgattccacc gtgtcgccga gcgccgtcgg ctcgaggcgg agcgtaaccc gtccgagctc
    15841 gatgttcgac aggtcggtga ggtcgtcgag gagccggccc aggcgctcgg tgtgccgcgc
    15901 ggcgatctcg aggaaccggc gtgcgtgcgc gggctcctcg agggccccgc cgagcagggt
    15961 ctcgaggtag ccccggatcg ccgtgagcgg cgtgcgcagc tcgtgcgaga cgttggccac
    16021 gaactccgtc cgcacctgct cgagccgccg cagctcggtg acgtcgtgca gcaccatgag
    16081 cgcgcccacc tcgtcggcgc cgagctgcag cggcaccgcc gtcgcctgca gcgtgcgatc
    16141 cacggggccg gcgagcgtca gctcgcgccg cgagacgacg ccctcgcccg cggcccggct
    16201 cgcccgcagg agttggtgca gctcggcgtt gcggatgacc tcgaggaagg gcttgcgctc
    16261 ggcccgggcc gtcccgaggc cgcagatcgc ccgcgcgcgc tcgttcatca gcaccacgtg
    16321 gtcgcggccg tcgacggcga tgacgccctc gaccatgccg tcgaggatcg ccgccacctt
    16381 cccctgctcg tgctcgaggt cgcccagctt ctcgcgcagc cgtgcggaca tcacgttgag
    16441 cgcccggccg agctggccga tctcgtcggg cgagcggacg gaggcccggg ccgtgaagtc
    16501 tccctcgctc attcgccggg cgatggcgtg catgtcgacg accggcttcg tcacccggcg
    16561 agccacgaag agcccgatac ccaaggccac cacgagcgcg accgcggtgc ccgcgagcat
    16621 cgctcgatgg accaccgcgt acgaatcggt caccagggac agcgggaacg cgaagcgcag
    16681 caccccgagc acccgcccgc cctcggccac cggcagggcc acgtagagca gcggcgcgtt
    16741 gaccgtcgcg ctccgcctga cgtgccgccc gatccgcccg tccagcgcgg cgcggacctc
    16801 ggggcggtcg ccgtggctct ccacgcgctg cagatcggcg accggcacct cggagtcgcc
    16861 gagcacccgc ccatcggcgg cgacgagggt cacgcgggcg cggctgggct cggccgcccg
    16921 cacggcgaag gcgcggacgg cgggtgccga cgcgggcccg gccagcagcc cgcgggcctc
    16981 gtcgtggagc agcgctccgg ccgtcgtcag gcgcgactcg agcgcctcga cggcgaacgt
    17041 ctccagcgcc tggtcgaggt agaggccggc gaaggtcacc gcgaccgcca cgaagccgac
    17101 cagcgtcagc gtgagcttga gcgcgatgga ccggcggagg aggtgcacca ggggctggcg
    17161 catcagtcgc cgtcgaagcg gtaaccgacg cccttgacgg tggcgatacg ccgcccctcg
    17221 tcgcccagct ttgcgcgcag gcgccgcacg tgcacgtcca ccgtccgcga ctcgacctca
    17281 ctggcgcggg cgtagcccca gacgtggtcg agcagatact cgcgcgagag cacacgcccg
    17341 ctcgcctcca tcagcgcgtg cagcaggtcg aactccttcg gcgtcagctc cacgcggacg
    17401 ccgccgacct ccaccgcgtg gcgcgcggga tcgacggtga cgtggcccgc gcgcagcgcg
    17461 gcggcatcgt cgcccgatcg gctgcggcgg agcacggcgc ggacgcgggc gaccagctcc
    17521 ttcggagaga acggcttggc cacgtagtcg tcggcgccga tctcgaggcc cacgacgcgg
    17581 tccacctcgt cggccttggc ggtgagcatg atgatcggca actgggccgt gcgcgggtcg
    17641 ccgcggagcc gacggcagac gtcgagcccg tccatctccg gcagcatgag gtccaggacc
    17701 acgaggtccg gccggctcgc ccgcgcctcc gcgagcgcct ggggccccgt cgacgccgtc
    17761 cggcagcggt agccttcgcg gccgaggtgg aagacgatca ggttgcggat gtcgggctcg
    17821 tcctccacca ccagcacttc ctgcgccatg ccgacactct agcttatcgg aggtgcctcg
    17881 acggcccctc cgagacgtcc cgcaggttgc gccggcgaag ccggcgctcg gacagcctgt
    17941 caggacacgc tgcggcgtcg ggcgggaaga ccacccggga ctatcggagc gcggcgagca
    18001 gcttcgccac ggcccaggag atcaggccgc agacggggaa ggtgaggatc caggcggtga
    18061 cgatctcgcc gccgacaccc cagcgtaccg ccgagaccct ccgggtgctg ccgaccccca
    18121 tgatcgccgt gttgatcgtg tgggtggtgc tgagcggcac gcccagccgc gtggccagct
    18181 cgatggtggt cgccgccgcc gtctccgccg cgaagccgtg gacgggatcg agcttggtga
    18241 ggccgagccc catggtcttg acgatccgcc agccgccgat ggccgtgccc acgcccatcg
    18301 tcgccccgca tagcaggatc acccacaggg ggacggcgaa cgtcggcagc acgccgccga
    18361 ggaccagcgt cagcgtgaac gcgccgatga acttctgccc gtcgttggac ccgtgactga
    18421 aggccatgaa cgccgccgac aggatctgca gccttccgaa gagcgcccgg acggtgccgg
    18481 gccgccatcg gcgaaagatc cagtagatac cgatcgtgag accgagcccg gcgaggaacc
    18541 cgaggaacgt ggaaaacacg agcccgagca gcaccttctg ccagccgtcc cacagcaaca
    18601 ccgagggccc cgccgtcgcg aggccggcgc cgctgagcgc agcgatcagc gcgtggctct
    18661 cgctggtcgg caagccgtag gaccacgcga gcgtggacca gaggacgata gcgatcatgg
    18721 ccgcggccac ggtggcgacg ttgatcgcct cgggcttgac catgcccttg ccgatcgtgg
    18781 ccgcgactgc cgtccccgac atcgcgccca gcacgttgag cacgctggcc atggcgagcg
    18841 cctggaaggg gccgagcacc cgggtcgaga cgacggtggc gatggagttg ggagcgtcgg
    18901 tccagccgtt gacgaactcc gcggcgagga cgaggagcag cacgacgacg aaccactcca
    18961 tccagtgact aggccatctt gaggatgatt ccctcgatca cgttggccac gtcctcgcag
    19021 cggtccgtga cgacctcgag cgtctcgtag atctccttcc acttgatgat ctcgatgggg
    19081 tcgccctgct gctcgaacag ctcggcgagg ctgtcgcgca ggaggttgtc ggcgctgttc
    19141 tccagccgat tgacctccac cgcgtgctcg tggaagcggg gatctctcgt gcgcagacag
    19201 cggaccgtat gatccacggc cctggtgatc tgcacgatga catcggtcag cgcgcggcag
    19261 gccgacgtgg gctccttgat ccggtagatg agcagccgct cggccgacgc ctcgatgtag
    19321 tcgagcacgt cgtcgaggcg gctcgccagc gcgtggatat cctcgcggtc cagcggcgtg
    19381 atgaacgtct gatgcagccg cttgacgacc tcgtgcgtga cctggtcacc ctggtgctcg
    19441 atctccttga tcgcgtgcat cttggcgcgc gcgtcggcga agtggtgcac gagctcgtgc
    19501 aggagctcgg cggcccggag aatgtgctgc gattgctgct cgaagtagtc gaagaatcgc
    19561 acctcgcgcg gaatca
//
LOCUS       DATIAZ010000124         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_293873, whole genome shotgun sequence.
ACCESSION   DATIAZ010000124 DATIAZ010000000
VERSION     DATIAZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293873"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VLF19_04230"
     CDS             <1..1344
                     /locus_tag="VLF19_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92492.1"
                     /translation="GNVMWNCGEAIQVVSDAVIQNNIILTSDVGITAAPHAQVNGVRN
                     VTIVNNTLYGHDVCLSIGWSGATNAILANNAVYCPSATAVSASLGSAVVRSNYITGSG
                     ASIDNVRFFAGGSATAAFANPAGRDLWPTASSVLRNTADASFVPPRDFNETTRTASFD
                     VGAYETEGLASNPGWRVVAGFKTVGSSPPPPPAQALTLIGAVLPSSRSVAVGQTATAF
                     AAVINVGPDRGLTCGLQLATPIAGAFAYQTTNPQTNALIGTANTPVDIEAGSLQTFVF
                     AVTPSAPLAPADVALSMDCANGAPAEVQPGLNTLLLSASTTPVPDVVAMAMTLSGDGI
                     VTATGAGADGVFSVATANVGLGATVTVTADTGSAAVPVSVQLCRTQPATGACLGAPAA
                     SVQTMLEAQGTATFGVFVRALGPIAFQPAVHRIHVRFRDGAGVIRGSTSVAVRTP"
     gene            1383..2621
                     /locus_tag="VLF19_04235"
     CDS             1383..2621
                     /locus_tag="VLF19_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSE92493.1"
                     /translation="MKITRVEALHCDGGWRPWTFVRIETDGGLVGWGECSDNRSPHGI
                     AGCVRDLADLLIGQDPRPVERLYWDMLRRVRQNSGGVSHKAIAGIELALWDIKAKALG
                     VPVYELFGGPLRDRVRLYWSHCGTTRARMGSVLGTPPLRTYDDIAALGREVVARGFTA
                     LKTNMVIPGEPATVYFPGFASGINTTDGAPTVEILEAIDRLIGTFRQAVGPKVGLCLD
                     LNYNFRTEGVLRIAKLLEKYDMQWLEYDNWDPDALLQIKQSTSTRLASCESLVTVRQY
                     RPFLERHAVDVAIVDVPWNGFSQAVQIGRLAEACEINVAPHNYYSHLADLHSLHLCAV
                     LPNVRIMEIDIDDVPWKGALVTKPPLIRDGNVWLTDAPGWGADIDERVLREHPWPRPG
                     DGGPRLFYGMDPGQMTARPG"
BASE COUNT          439 a         1001 c         1068 g          408 t
ORIGIN      
        1 ggcaacgtga tgtggaactg cggcgaggcg atccaggtcg tgtcggatgc cgtcattcag
       61 aacaacatca tcctgacctc cgacgtcggc atcaccgccg cgccgcacgc ccaggtgaac
      121 ggggtgcgga acgtcaccat cgtcaacaac acgctctacg gccacgacgt gtgtctctcg
      181 atcgggtggt cgggcgcgac gaacgcgatc ctggccaaca acgccgtgta ctgcccgtcg
      241 gcgacggccg tcagcgcaag tctcggcagc gccgtggtga gatcgaacta catcaccggg
      301 tcgggcgcga gcatcgacaa cgtgcgcttc ttcgccggtg gcagcgccac ggccgcgttc
      361 gcgaacccgg cgggacgcga cctgtggccg acggcgagct ccgtgttgag gaacaccgcc
      421 gatgcctcgt tcgtgccgcc gcgggacttc aacgagacca cgcggacggc ctcgttcgac
      481 gtcggcgcct acgagacgga ggggctggcg tcgaaccccg gctggcgggt cgtggccggc
      541 ttcaagacgg tcgggagcag cccgccgccc ccaccggcgc aggcgctgac cttgatcggg
      601 gcggtgctgc catcgagccg gtcggtcgcg gtgggccaga ctgcgacggc gttcgcggcg
      661 gtgatcaacg tgggaccgga tcgcgggctg acgtgcgggc tgcagctggc gacgccgatc
      721 gcgggggcgt tcgcgtacca gacgacgaac ccgcagacca acgcgctgat cggcacggcg
      781 aacacgccgg tggacatcga ggccgggtcg ctgcagacgt tcgtgttcgc ggtgacgccg
      841 tcggcgccgc tggcgccggc ggacgtggcg ctctcgatgg actgcgcgaa cggcgcgccg
      901 gcggaggtcc agccggggct gaacacgctg ctgctgtcgg cgtcgacgac cccggtgccg
      961 gacgtcgtgg cgatggcgat gacgctgagc ggcgacggga tcgtgacggc gaccggggcc
     1021 ggggcggacg gcgtgttctc ggtggcgaca gccaacgtcg ggctcggggc gaccgtgacg
     1081 gtgacggcgg acaccgggag cgccgcggta ccggtgagcg tgcagctgtg ccggacgcag
     1141 ccggcgacag gggcgtgtct cggggccccg gcggcgagcg tgcagacgat gctcgaggcg
     1201 caggggacgg cgaccttcgg ggtgttcgtg cgggcgctgg ggccgatcgc gttccagccc
     1261 gcggtgcacc ggatccacgt gcggttccgg gacggcgccg gggtcatccg tggctccacg
     1321 agcgtggccg tccgcacccc gtagctttgc gacgctgacc tgctctggcg ggaggacggg
     1381 caatgaagat cacgcgcgtg gaggcgctgc actgcgacgg gggctggcgg ccgtggacgt
     1441 tcgtgcgcat cgagaccgac ggcggcctcg tcggctgggg cgagtgcagc gacaaccgga
     1501 gcccccacgg catcgccgga tgtgtgcgcg acctcgccga cctcctgatc ggccaggacc
     1561 cgcggccggt cgagcgcctg tactgggaca tgctgcgccg ggtgcgccag aacagcgggg
     1621 gcgtctcgca caaggccatc gccggcatcg agctggccct gtgggacatc aaggccaagg
     1681 cgctcggggt gccggtctac gagctgttcg gcggcccgct gcgcgaccgc gtgcggctct
     1741 actggtcgca ctgcggcacg acacgagcgc gcatgggcag tgtgctgggc acgccgccgc
     1801 tccgcaccta cgacgacatc gcggcgctgg ggcgcgaggt ggtcgcgcgg ggcttcaccg
     1861 ccctcaagac caacatggtc atccccggcg agccggccac cgtgtacttc cccggcttcg
     1921 ccagcggcat caacaccacg gacggcgcgc ccacggtgga gatcctcgag gccatcgacc
     1981 gcctcatcgg cacgttccgc caggcggtcg gcccgaaggt cggcctgtgc ctggacctca
     2041 actacaactt ccgcaccgag ggcgtgctcc ggatcgccaa gctgctcgag aagtacgaca
     2101 tgcagtggct ggagtacgac aactgggatc cggacgccct gctccagatc aagcagtcca
     2161 cctcgacgcg gctggcctcc tgcgagagcc tggtgacggt gcgccagtat cgcccgttcc
     2221 tcgagcgcca cgccgtcgac gtggccatcg tcgacgtgcc gtggaacggc ttctcgcagg
     2281 ccgtgcagat cgggcggctg gccgaggcgt gcgagatcaa cgtcgccccg cacaactact
     2341 acagccacct ggccgacctg cactccctgc atctctgcgc ggtgctgccc aacgtgcgca
     2401 tcatggagat cgacatcgac gacgtgccgt ggaagggtgc gctcgtcacg aagccgcccc
     2461 tgatccgtga cggcaacgtc tggctcaccg acgcgccggg gtggggcgcg gacatcgacg
     2521 agcgcgtgct gcgcgagcat ccgtggccgc gccccggcga cggggggccg cggctgttct
     2581 acggcatgga ccccgggcag atgacggcgc gcccgggatg agccgcctcg ggcgctcccc
     2641 gcaggagcgc ccgggaactg gccacgccgt cggagctgcc cactcgtccg gagaccgaca
     2701 aaggcccgcg gtcgcttgac aaagccgccg atgagcccgt acttggagaa gcatgcccta
     2761 catcatcggc atcgacaccg gcggcacgtt caccgactgc gtggtcatgg acgctggtgg
     2821 gcgcatcgtc accgccaagg cctcctccac ccccgatgac ttctccgagg gcgtcatgga
     2881 gtcgctgggg ctggccggcg agaagctcgg gctcac
//
LOCUS       DATIAZ010000125        12611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1214502, whole genome shotgun sequence.
ACCESSION   DATIAZ010000125 DATIAZ010000000
VERSION     DATIAZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12611
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214502"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VLF19_04240"
     CDS             <1..224
                     /locus_tag="VLF19_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSE92494.1"
                     /translation="LLYLGAVTTAGAYALYAAGLRMVPASVAGVVTLLEPLTATLLAV
                     LVFGERLGLTGAIGAALLLSAIALLSRRP"
     gene            complement(160..1053)
                     /locus_tag="VLF19_04245"
     CDS             complement(160..1053)
                     /locus_tag="VLF19_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013067739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSE92495.1"
                     /translation="MKLVRYGRPGTEKPGMIDEDGKLRDLSRVIRDVTPDVLSGAALN
                     RLRATRIGRLPVVRGTPRLGVPLAGIGKIICIGLNYTDHAEEVGLPLPKEPLFFIKAT
                     SALTGPTDPIVRPPGATKLDYEVELAVVIGREARYVEEAGALGYAAAYCVLDDVSERE
                     FQMNRGGTTTKGKSADTFAPVGPWLVTADEVRDPQALEVWTAVSGKPRQRGKTSNMVF
                     SVAHLIAYVSQFVSLQPGDVISTGTPAGVGHGARPPRYLRPGDVVEMGVTGLGAQRNV
                     VTDAGTGARSRTAAAPRRSRR"
     gene            complement(1078..2115)
                     /locus_tag="VLF19_04250"
     CDS             complement(1078..2115)
                     /locus_tag="VLF19_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92496.1"
                     /translation="MSAEQVVEVEDSPEAVLAAFVEREWCDGLPVVPPTEDRVHAMLG
                     GRDPARSLGAMPPLWRAATLEKLAVNAVMAGCLPEHFPVVVAAVEAMLDPEFNLYGVQ
                     ATTHPVAPLVIVSGACGRRIGLHAGSGCFGPGFRANATIGRAIRLVLMNIGGAWPGRH
                     DMATQGSPAKFSYCIAEHEEASPWGPLAAGDVVTVYGGEAPHNVNDHCSTTGSGILTT
                     IAHTAVTLGSNVGWYFSQSQLLVVLGPEHAATIAGDGLTRADVQRFVYEHARLPLGTL
                     KLGGMWGMQDWPPWMMAIRDDEARPPRVPSPDDVFVIVAGGPGKHSSVVPNCCFSRAV
                     ARPIDSSAGRS"
     gene            complement(2132..2335)
                     /locus_tag="VLF19_04255"
     CDS             complement(2132..2335)
                     /locus_tag="VLF19_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92497.1"
                     /translation="MAKAGTPAALIATTAFQGLAVRQLAARGISGLPLLVIEHPLGGE
                     RPEGVSRRAWQAMEQLASLLGRA"
     gene            complement(2375..2662)
                     /locus_tag="VLF19_04260"
     CDS             complement(2375..2662)
                     /locus_tag="VLF19_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92498.1"
                     /translation="MARIRVLSPVGADPLEALTVPALPLELAGRTVGFLDNTKANFDR
                     LAGDIGRLLRERHGVKDVVHRRKPNASTPAPPELVAEVAKSCDIVFAGSAD"
     gene            complement(2694..2912)
                     /locus_tag="VLF19_04265"
     CDS             complement(2694..2912)
                     /locus_tag="VLF19_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92499.1"
                     /translation="MSDTWEVLTLRGLAATDERAEEFTGTLVIHRVGSREPVESITVQ
                     VKRSILTELHGTLGRLLKRSIGFRHGAR"
     gene            2942..4387
                     /locus_tag="VLF19_04270"
     CDS             2942..4387
                     /locus_tag="VLF19_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HSE92500.1"
                     /translation="MILALDVGTSSARAALYDDAARPVPGRFHQVHYRPAVTPDGGVE
                     HDPATLLDAAAACVDAVVAHGGLPDVAGVAIATFWHGLLGMDGSGRACTPLFTWADTR
                     AAGDAGLLREALDEEALRRRTGCHLHASYWPAKLRWLARERPHLVARVARWGSFGEYL
                     ELALFGEAATSVSMASGTGLLDQQTMRWDGEALAAAELDAAHLFPLRDRRDGRVGLRP
                     AWARRWPALRGRPWFPAVGDGAAGNVGSGCVTSARLALNVGTSIALRMAAPDLGTPPR
                     GLWRYRLDRGLSLVGGALSEGGNVYAWCRELLRLPDDAALEAALAVREPDAHGLTVLP
                     FVAGERSPGWRGDRRAVIAGLKLDTGPLDVLAAALEAVALRIALVYDLLRPLAAPRHV
                     IVASGGAVARAGAWAQTIADALGQPLIVCREEEATSRGAAVLALEALGRVPDLGAAVP
                     PAGDTIEPVPAHHARYRAALERQRRLDTRLG"
     gene            4556..5296
                     /gene="pgl"
                     /locus_tag="VLF19_04275"
     CDS             4556..5296
                     /gene="pgl"
                     /locus_tag="VLF19_04275"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSE92501.1"
                     /translation="MDPKPEITVFDDTAALADAAARAIVDAAGEAAAARGRFTIALSG
                     GATPRETYTRLAQPLFADAVPWTRTFMFFGDERWVPADHPQSNHHLAHETLLGKVPVP
                     PGQVFRMLCGGDDPDAAAAAYARTLADVFGLRRGALPRFDLVLLGMGLDGHTASLFPG
                     SPALKEVFRHVVAVHAAAAAIPQRLTLTFPVLNAAGCAVFLVAGAEKAKAVKAVFADG
                     ALLPAGMVRPTDGRLVWMLDRAAAARLG"
     gene            5296..6765
                     /gene="zwf"
                     /locus_tag="VLF19_04280"
     CDS             5296..6765
                     /gene="zwf"
                     /locus_tag="VLF19_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSE92502.1"
                     /translation="MPEQAFSLTIFGASGDLTRRKLIPALFALYASRTLPEPFAIVAV
                     ARTGMASEEFRRRMREAVTEFGRVQPPSEAVWDRFATALHYLSGDPKDPALYTRLEAF
                     LREVEPGRPENRLFYCATPPSLYDDIVEHLGASGLARGEGGWTRIVVEKPFGRDLESS
                     RALNRQLAGVFREEQVYRIDHYLGKETVQNLLVLRFGNGIFEPLWNRNHVDHVQIMVA
                     ESIGVETRGAYYEEAGALRDMMQNHLLQLLCLIAMEAPVTFDAGPVRDEKNKVMQAIR
                     PIERVDEVAVRGQYDGGFVDGRPAVAYRSEKGVNPDSRTETYAALKLYVDNWRWAGVP
                     FYLRTGKRLARRASEIVVQFRRTPHMIFRRQPAGPEANVLVVRIQPDEGMALRVAVKV
                     PGPELKMSPVTLDFRYGEVFGGQSPEAYERLLLDAIGGDTTLYARGDWVEQAWAILDP
                     VLATWQADPEAPPLYDAGSWGPAEADAFIARDGRAWRNP"
     gene            complement(6817..7221)
                     /locus_tag="VLF19_04285"
     CDS             complement(6817..7221)
                     /locus_tag="VLF19_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92503.1"
                     /translation="MMKYMLAVVLVAGLLTGCATSSRTVRSEFEDIPVPKGLTYVEDR
                     STIIESPAVKAARLIYRGRIEPVSLGVAMRSTLESNGWRNVSVTSTGGEGTTQVYEKS
                     GNSLQVRLWEGWYYTYVELTVSRSLGGSQAAK"
     gene            complement(7283..8878)
                     /gene="serA"
                     /locus_tag="VLF19_04290"
     CDS             complement(7283..8878)
                     /gene="serA"
                     /locus_tag="VLF19_04290"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HSE92504.1"
                     /translation="MRVLVADPVAAQGIAHLRERGFTVDELPRAAAETLHARLPDYEA
                     LITRSGTAVTPELLRHAPRLRIIGRAGVGVDNIDVDACSRRGIVVVNAPYGNVVSAAE
                     HTVGMLLALVRRIPDGHASLKAFTWDRSIYGAELFRKTVGVVGLGKVGSRVAARLRSF
                     EAELLVHDPYVPESRARDLGARLTDFDTLLCKSDVITFHVPLTDETDHMVTARELART
                     RRGVRIVNCARGGIVDEGDLLAALETGHVAGAALDVWSEEPPRSERLRRLIAHPRVVV
                     TPHLGANSSEAQVNVALDVARQIVAFRDGQLVEFAVNIPVGDPAALAGLRPFISLAER
                     LGQFCVQLDPEHLERVEVAVAGAVADTDPELLARAVLAGLLAPVMDGPVNLVNAHLMA
                     RERGVAVTVTREDETHGYGSLLTVTVHTREGRKIIAGTVFDGQPRIVRMRDLHIEFTP
                     EGYVLVLSYEDRPGMVGRIGTTLGEMNVNIASMHVGRKAKRGRAIVVLILDEDLSSEQ
                     LAVVARSIEADFVRLVRLTDPKS"
     gene            complement(8882..9640)
                     /locus_tag="VLF19_04295"
     CDS             complement(8882..9640)
                     /locus_tag="VLF19_04295"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSE92505.1"
                     /translation="MFDLKGKVAIVTGGNGGIGLGMARGLAAAGARVVVAGRNAVKSK
                     AAVAQLARLGPDPLAIEVDVASEPSVAALVRGTTERCGRLDILVNNAGINVRKPAHEL
                     TLEEWRQVLDTNLTSAFLCSRAAYPAMKAAGGGKIVNIGSMMSIFGASFVPAYGASKG
                     GIVQLTRALACGWAPDNIQVNAVLPGWIDTDLTRRAREQVPGLNDNVLRRTPAGRWGT
                     IEDMAGVAVFLASRASDFVTGAAIPVDGGFSVQG"
     gene            9840..10718
                     /locus_tag="VLF19_04300"
     CDS             9840..10718
                     /locus_tag="VLF19_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92506.1"
                     /translation="MTQTRPVDSHRFWGVVLAGNLDERQPRRPARAVPDERVREQRAL
                     FRQMLERATWLVPAERLVAVLSRDHAAYYDSDLQGLGPIHTVVQPAWRGSAAGLFLPI
                     LKIAAGDPDATVAIFPGGQPVYGDAQLMTSVHKAVRAVSVRPELPVVIGAAPRGPDLA
                     QAWIEPAEAVEGLEPYAVRAVRRFVHRPSRAEAASLWEGDGLVNTHVVIAKARALIEL
                     GGRYLPDVLETFEPLGSAFGTPEEALLCEAVYEEMPYASVAHALFVRGRDVAVLAAPH
                     VRTRLEAPALADALAS"
     gene            complement(11084..11713)
                     /locus_tag="VLF19_04305"
     CDS             complement(11084..11713)
                     /locus_tag="VLF19_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSE92507.1"
                     /translation="MGGLPEDLDRLLHDLRGPLNAMQMHVELLKRLDVGEPAALASLE
                     SIAQEVSRLATMLPAAFHVMAVELHEREAHDLRAIVETALAEHELRGVVVAPGPWPRV
                     PGDARLLRLAVAHLIRNALAADGGRAPEIDARDAGDSAVLIVRDWGSGLRTTNPRALI
                     RLGYRPGDRPRVGLVTVERVARLHGGRIEFATPPGGGTEVRLTLPAAGS"
     gene            complement(11713..>12611)
                     /locus_tag="VLF19_04310"
     CDS             complement(11713..>12611)
                     /locus_tag="VLF19_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE92508.1"
                     /translation="ATSTAPVLVYGESGTGKELVARTIHDLSNRRSKPFVAINCAAIP
                     ETLIESELFGHERGAFTGATERRLGCFELADGGTLFLDEIAEMDNSTQAKLLRVLQEG
                     SFRRVGGGKGEIQVDVRVIAATNRVPTDAMAQGQLREDLFYRLNVFAIRLPALRERKE
                     DIPLLGKTFIDEFNRQDNRQVRGLNAEAEKALDRYAWPGNVRELRNVIQRAVVLSGTG
                     LIGVEHLPDNVLRGAEPAKAVATGSIRTIRELEREEILRALEETSQDKRKAAALLGIS
                     LKTLYNKLAKYGIQAVKSARIT"
BASE COUNT         1689 a         4612 c         4463 g         1847 t
ORIGIN      
        1 ggctcctcta tctcggcgcg gtgaccaccg ccggcgccta tgcgctctac gccgcgggcc
       61 tgcgaatggt accggcgtcg gtcgcggggg tcgtcacgct cctggagccg ctgaccgcaa
      121 cgctgctggc cgtgctcgtc ttcggcgagc gcctgggact caccggcgcg atcggcgcgg
      181 cgctgctgct gtccgcgatc gcgctcctgt cccggcgtcc gtgacgacgt tccgctgcgc
      241 cccgagcccg gtgacgccca tctcgacgac gtctcccggc cgcaggtagc gcggcggcct
      301 ggcgccgtga cccacgcccg ccggtgtgcc ggtcgagatg acgtcgccgg gctgcagcga
      361 gacgaactgg ctgacgtagg cgatgaggtg ggccaccgag aacaccatgt tcgaggtctt
      421 gccgcgctgc ctcggtttgc cgctgacggc ggtccacacc tccagcgcct gcgggtcccg
      481 cacctcgtcg gccgtgacca gccatggccc gacgggcgcg aaggtatcgg cgctcttgcc
      541 cttggtggtc gtgccgccgc ggttcatctg gaactcgcgc tccgacacgt cgtccagcac
      601 gcagtacgcg gccgcgtagc cgagcgcgcc ggcctcctcg acgtaccgcg cctctcgccc
      661 gatcaccacc gcgagctcga cctcgtagtc gagcttcgtg gccccggggg gacgcacgat
      721 cgggtcggtc ggtccggtga gcgcgctcgt cgccttgatg aagaacagcg gctccttggg
      781 caagggcagc ccgacctcct ccgcgtggtc cgtgtagttg agcccgatgc agatgatctt
      841 gccgatgccg gcgagcggca cgccgaggcg cggggtgccg cgcacgacgg gcagccggcc
      901 gatcctcgtc gcgcgcagcc gattgagcgc ggcgcccgac aggacgtccg gcgtcacgtc
      961 gcggatcacg cgggagagat cgcgcagctt gccgtcctcg tcgatcatgc cgggcttctc
     1021 ggtccccggc cggccgtagc ggacgagctt catggtcggc ctcctgcggc cggggcgtca
     1081 gctccggccg gcggacgagt cgatgggccg cgccaccgcc cggctgaagc agcagttcgg
     1141 caccacgctc gagtgcttgc cgggaccgcc ggcgacgatc acgaagacgt cgtcgggcga
     1201 gggcacgcgc ggcggtcggg cctcgtcgtc gcgaatggcc atcatccacg gtggccagtc
     1261 ctgcatgccc cacatgccgc cgagcttcaa tgtcccgagc ggcagccggg cgtgctcgta
     1321 gacgaatcgc tgcacgtcgg cacgcgtgag accgtcgccg gcgatcgtgg ccgcgtgctc
     1381 gggaccgagc acgacgagga gctggctctg cgagaagtac cagcccacgt tcgagccgag
     1441 cgtgaccgcc gtgtgggcga tggtggtgag gatgcccgac cccgtcgtcg agcaatggtc
     1501 gttgacgttg tggggcgcct cgccgccgta gacggtcacg acgtcgccgg cggcgagcgg
     1561 cccccacggc gaggcctcct cgtgctcggc gatgcagtag ctgaatttcg ccgggctgcc
     1621 ctgggtggcc atgtcgtggc ggccgggcca ggcgccgccg atgttcatga ggacgagcct
     1681 gatggcgcgc ccgatggtgg cgttggcgcg aaagcccggt ccgaagcagc cgctgccagc
     1741 gtggagaccg atgcgacggc cgcaggcgcc gctcacgatc accagcggcg cgacggggtg
     1801 cgtggtggcc tggacgccgt acaggttgaa ctcggggtcg agcatggcct cgacggccgc
     1861 gacgacgacg ggaaagtgct cgggcaggca gccggccatc acggcgttca cggccagctt
     1921 ctcgagggtc gccgcgcgcc agagcggcgg catcgcgccc agcgaccgcg ccggatcgcg
     1981 gccgccgagc atcgcgtgca cgcggtcttc ggtcggcggg accacgggca agccgtcgca
     2041 ccactcgcgc tcgacgaagg cggcgagaac ggcctcgggg ctgtcctcga cctccacgac
     2101 ctgctcggcg ctcacgggcg gccctcgccg ctcacgcgcg tccgagcagg ctcgccagct
     2161 gctccatcgc ctgccacgcc cgtcgcgaca cgccctcggg ccgctcgccg cccagcgggt
     2221 gctcgatgac tagcagcggc agcccgctga tcccgcgcgc cgccagctgg cgcacggcca
     2281 gtccctggaa ggcggtcgtg gcgatcagcg ccgccggcgt gccggccttc gccacctcga
     2341 tcgcgtcgtg gagactccac gacgtgcagg accctcagtc ggccgacccg gcgaagacga
     2401 tgtcgcagct cttggccacc tcggccacga gctcgggcgg cgctggtgtc gaggcgttcg
     2461 gcttccggcg atggacgacg tccttcacgc cgtgccgctc gcgcagcaga cgtccgatgt
     2521 cgccggccag ccggtcgaag ttcgccttgg tgttgtcgag gaacccgacg gtgcgcccgg
     2581 ccagctcgag cggcagcgcg ggcacggtga gcgcctccag cggatcggcg ccgacggggc
     2641 tcaggacacg aatgcgggcc atggaagcct cctcgcgagg tcgtggaagt tcgtcaccgg
     2701 gcgccgtggc ggaaaccgat ggagcgtttc aggagacgtc cgagcgtgcc gtgcagctcg
     2761 gtgaggatgg accgcttcac ctgcacggtg atcgactcca ccggctctcg actgcccacc
     2821 cggtggatca ccagcgtccc cgtgaactcc tcggcacgct cgtccgtggc cgccaggccg
     2881 cgcagcgtca gcacttccca ggtgtcgctc atggaggcta tcttaacccg gggcccgtcc
     2941 catgatcctc gcgctcgacg tcggcacctc ctcggcgcgc gccgcgctct acgacgatgc
     3001 ggcgcgtccg gtgcctggac gcttccacca ggtccactac cggccggccg tcacccccga
     3061 cggtggtgtc gagcacgacc ctgccaccct gctggatgcc gccgccgcgt gcgtggacgc
     3121 ggtcgtcgcg cacggcgggt tgcccgacgt cgccggtgtg gcgatcgcca ccttctggca
     3181 cggcctgctc gggatggacg gctcgggccg ggcctgcacg ccgctcttca cgtgggcgga
     3241 cacgcgggcg gccggcgacg ccggtctgct gcgagaggcg ctcgacgagg aggcgctccg
     3301 tcggcgcacg ggctgccacc tgcacgcctc ctactggccc gcgaagctcc ggtggctcgc
     3361 ccgcgagcgg ccccatctcg tggcgcgggt ggcgcgctgg ggctccttcg gcgagtacct
     3421 cgaactggcg ctgttcggag aggcggccac gagcgtgtcg atggcgtcgg ggacggggct
     3481 gctcgaccag caaacgatgc gctgggacgg cgaggcgctc gccgccgccg agctggacgc
     3541 cgcacacctc ttccctctcc gcgaccgccg cgacggccgg gtcggcctcc ggccggcgtg
     3601 ggcgcgccgc tggccggccc tccgcgggcg gccgtggttc ccggcggtcg gtgacggcgc
     3661 cgccggcaat gtcggctccg ggtgcgtgac gagcgcgcgg ctggcgctca acgtcggcac
     3721 ctcgatcgcg ctccgcatgg cggcgccgga cctcggcacg ccgccgcggg gcctctggag
     3781 gtaccggctc gaccgcgggc tctcgctcgt cggcggcgcg ctctccgagg ggggcaacgt
     3841 gtacgcctgg tgccgggaac tgctgcggct gcccgacgac gccgcgctgg aggccgcgct
     3901 ggcggtgcgc gagccggatg cccacggtct caccgtgctg ccgttcgtcg ccggcgagcg
     3961 ctcgcccggc tggcgcggcg atcgacgggc ggtgatcgcc ggcctcaagc tcgacaccgg
     4021 gcctctcgac gtgctcgccg ccgcgctgga ggccgtcgcg ctccgcatcg cgctggtcta
     4081 cgacctgctc cggccgctgg cggccccgcg ccacgtcatc gtcgcctccg gcggtgccgt
     4141 cgcgcgggcg ggcgcctggg cgcagacgat cgccgacgcg ctgggccaac cactgatcgt
     4201 ctgccgcgaa gaggaggcga cgagccgcgg ggccgcggtg ctcgcgctcg aagcgctcgg
     4261 ccgcgtgccc gacctcggcg ccgccgtgcc gcccgcgggc gacaccatcg agcccgtccc
     4321 cgcccatcac gcgcgttatc gcgcggctct ggaacgccag cgccgcctcg acacgcgact
     4381 cggctagagc gcgcggcggg ggcccgacgc tccttcgcac ggcgaggatg gggggtaccc
     4441 atgtcggtta gcgaggcgcg tcacgacccc cgccgcgggg tgccgagcgt tgggggccat
     4501 gtcggggccc catgtcgatg atcgccgctt gaacgggcaa ggtatgatcg agtcgatgga
     4561 tcccaagccc gagatcaccg tgttcgacga cacggccgcg ctcgcggacg ccgctgcgcg
     4621 cgcgatcgtc gacgccgcgg gagaggccgc ggccgcgcgg gggcgcttca cgatcgcgct
     4681 ttcaggcggc gcgacgccgc gcgagaccta cacccgcctg gcgcagccgc tcttcgccga
     4741 cgccgtgccc tggacgcgga cgttcatgtt cttcggcgac gagcgctggg tgccagccga
     4801 ccacccacag tccaatcacc acctggcgca cgagacgctg ctcggcaagg tgccggtgcc
     4861 gccaggccag gtgttccgca tgctctgcgg gggcgacgac ccggacgcgg cagccgcagc
     4921 ctacgcgcgc acactcgccg acgtcttcgg gctgcggcgc ggcgcgctcc cgcgcttcga
     4981 cctcgtgctg ctcggcatgg gtctcgacgg ccacaccgcc tcgctcttcc cgggctcgcc
     5041 cgcgctcaag gaggtcttcc gccacgtggt cgcggtccac gccgcggcgg cggccatccc
     5101 ccagcggctg acgctcacgt ttcccgtgct gaacgcggct ggctgcgcgg tctttctcgt
     5161 tgccggcgcc gagaaggcca aggccgtcaa ggcggtgttc gccgacgggg cgctcctgcc
     5221 tgccgggatg gtccgtccga ccgacggccg gctcgtctgg atgctcgatc gcgccgccgc
     5281 cgcccggttg ggctgatgcc cgagcaggcc ttctcgctga cgatcttcgg tgcctccggc
     5341 gacctcacgc gccgcaagct gattcccgcg ctcttcgctc tctacgcctc gcgcacgctg
     5401 cccgaaccct tcgcgatcgt ggccgtggct cggacgggga tggccagcga ggagttccgc
     5461 cggcgcatgc gcgaggccgt cacggagttc ggccgtgtgc agccgccgtc ggaggccgtg
     5521 tgggaccggt tcgccaccgc cctgcactac ctgtcgggcg acccgaagga tcccgccctc
     5581 tacacgcggc tggaggcctt cctgcgcgag gtcgagcccg ggcggccgga gaaccgtctc
     5641 ttctactgcg ccacgccgcc gagcctctac gacgacatcg tcgagcacct gggcgcctcc
     5701 ggcctcgctc gtggcgaagg gggctggacc cgcatcgtcg tggaaaagcc gttcggacga
     5761 gatctggaat cgtcgcgcgc gctcaatcgc cagctcgccg gcgtgtttcg cgaagagcag
     5821 gtctaccgga tcgaccacta cctcggcaag gagacggtcc agaacctgct cgtgctgcgc
     5881 ttcggcaacg gcatcttcga gccgctctgg aaccgcaatc acgtggatca cgtgcagatc
     5941 atggtcgccg agtcgatcgg cgtggagacg cggggcgcct actacgagga ggccggtgcg
     6001 ctccgcgaca tgatgcagaa ccacctgctg cagctcctct gtctcatcgc gatggaggcg
     6061 ccggtcacat tcgacgccgg cccggtgcgc gacgagaaga acaaggtcat gcaggccatc
     6121 cggcccatcg agcgcgtgga cgaggtggcc gtgcgggggc agtacgacgg gggcttcgtg
     6181 gacgggcggc ccgccgtcgc ctatcgcagc gagaagggcg tcaacccgga ctcgcgcacc
     6241 gagacctacg ccgcgctgaa gctctacgtg gacaactggc ggtgggcggg cgtgccgttc
     6301 tatctgagga ccggcaagcg gctggcccgc cgcgcgagcg agatcgtcgt ccagttccgc
     6361 cgcacaccgc acatgatctt ccggcggcag ccggcggggc ccgaggccaa cgtgctggtg
     6421 gtgcgaatcc agcccgacga gggcatggcg cttcgggtgg ccgtgaaggt gcccggcccc
     6481 gagctcaaga tgagcccggt gacgctcgac ttccgctacg gcgaggtctt cggcggccaa
     6541 tcgccggagg cttacgagcg actcttgctc gacgcgatcg ggggtgacac caccctctac
     6601 gcacgcggcg actgggtcga gcaggcgtgg gccatcctcg acccggtgct cgccacgtgg
     6661 caggccgacc cggaggcgcc accactctat gacgccggtt cgtggggccc ggccgaagcc
     6721 gacgccttca tcgctcgcga cggacgcgcc tggcggaatc cgtgacaggc ggccccgcac
     6781 gccgcaccgc accgcggcgt gccacgatga cccgacctac ttcgcggctt gcgagcctcc
     6841 gagcgagcgg ctgacggtga gctcgacgta ggtgtagtac cagccctccc acaggcgcac
     6901 ctggagcgag ttgccgctct tttcgtagac ctgggtcgtg ccctccccgc cggtgctcgt
     6961 cacgctcacg ttgcgccagc cgttggactc gagcgtcgag cgcatggcga cacccaggct
     7021 gacgggctcg atccgaccac ggtagatcag gcgagccgcc ttgaccgcgg gcgactcgat
     7081 gatcgtcgag cggtcctcga cgtacgtgag ccccttgggg acgggaatgt cctcgaactc
     7141 gctccgcacc gtgcgcgagg aggtcgcgca cccggtcagc agaccggcca ccaggacgac
     7201 ggcgagcatg tatttcatca tggcgccgag gctagcagcg tggcccctcc gccaccaagg
     7261 aaattcggga ggcctcgccc ggtcaggact tggggtcggt gagccggacg agtctcacga
     7321 agtcggcctc gatgctccgg gccacgaccg cgagctgctc ggatgagaga tcctcatcga
     7381 ggatcagcac gacgatggca cggccgcgct tggccttgcg cccgacgtgc atggaggcga
     7441 tgttcacgtt catctcaccg agcgtggtgc cgatgcgccc gaccatgccc ggccggtctt
     7501 cgtacgacag cacgaggacg tagccctcgg gcgtgaactc gatgtggagg tcgcgcatcc
     7561 gcacgatgcg cggctggccg tcgaacaccg tgcccgcgat gatcttgcgt ccctcccgcg
     7621 tgtgcacggt cacggtcagc aggctgccgt agccgtgcgt ctcgtcctcg cgggtgacgg
     7681 tgacggcgac cccgcgctcg cgcgccatca ggtgggcgtt gacgaggttc accggaccgt
     7741 ccatcacggg ggcgagcagg cccgcgagca cagcccgcgc gagcagctcg ggatcggtgt
     7801 cggccaccgc gcccgccacg gcgacctcga cccgctcgag gtgctcggga tcgagctgga
     7861 cacagaactg tcccaggcgc tcggccagcg agatgaaggg tcgcagcccg gccagcgccg
     7921 ccgggtcacc caccggaatg ttcacggcga actcgaccag ctggccgtcg cggaaggcga
     7981 cgatctgccg cgcgacgtcg agcgccacgt tcacctgggc ctcgctggag ttggcaccca
     8041 ggtgcggcgt cacgacaacg cgtggatggg cgatcagccg gcggagccgc tcactccgcg
     8101 gcggctcctc gctccacacg tcgagggcgg cgccggccac atgccccgtc tccagcgccg
     8161 ccagcaggtc gccctcgtcc acgatgccgc cgcgcgcaca gttcacgatt cgcacgccgc
     8221 gcctggtccg cgccagctcc cgcgccgtca ccatgtggtc agtttcgtcg gtcagcggca
     8281 cgtgaaaggt gatcacgtcg gacttgcaca gcagcgtgtc gaaatcggtg agccgggcgc
     8341 ccaggtcccg cgcgcggctc tcggggacgt aggggtcgtg cacgagcagc tccgcctcga
     8401 agctgcgcag ccgcgcggcc acgcgggagc cgaccttgcc gaggccgacc acgccgaccg
     8461 tcttccggaa cagctcggcg ccgtagatgg agcggtccca ggtgaatgcc ttgaggctcg
     8521 cgtggccgtc ggggatgcgg cgcaccagcg ccagcaacat gccgaccgtg tgctcggcgg
     8581 ccgagacgac gttaccgtac ggcgcgttga cgaccacgat gcctcggcgg gagcaggcgt
     8641 ccacgtcgat gttgtccaca cccacccccg cccggccgat gatccggagc cggggggcgt
     8701 ggcgcagcaa ctccggggtc accgcggtgc ccgagcgggt gatgagcgcc tcgtagtccg
     8761 gcagacgcgc gtggagcgtc tcggcggcgg cccggggcag ctcgtccacg gtgaagcctc
     8821 gctcgcgcag gtgggcgatg ccctgtgccg cgaccgggtc ggcgacgagg acgcgcacgg
     8881 ctcagccctg gacggagaac ccgccgtcca cgggaatggc cgcgccagtc acgaaatccg
     8941 acgcccggct cgccaggaac accgccacac ccgccatatc ctcgatcgtg ccccagcggc
     9001 cggccggtgt ccggcgcagc acgttgtcgt tgagccccgg gacctgctcc cgcgcccgac
     9061 gcgtcaggtc ggtgtcgatc cagcccggca gcaccgcgtt gacctggatg ttgtcgggcg
     9121 cccacccgca cgcgagcgcc cgggtgagct gcacgatccc gcccttgctg gcgccgtacg
     9181 cgggcacgaa gctcgcaccg aagatcgaca tcatcgaccc gatgttcacg atcttaccgc
     9241 cgcccgccgc cttcatcgcg ggataggcgg ccctggagca gaggaacgcc gaggtgagat
     9301 tcgtgtccag cacctggcgc cactcctcga gcgtcagctc gtgggccggc ttccggacat
     9361 tgataccggc gttgttcacg aggatgtcga ggcgcccgca gcgctcggtc gtgccgcgca
     9421 ccagggccgc gaccgacggc tcgctcgcga cgtcgacctc gatcgcgagc ggatcggggc
     9481 cgaggcgcgc gagctgggcg accgcggcct tcgacttgac ggcgttgcgg cccgccacca
     9541 ccacccgcgc gccggcggcc gcgagccccc gcgccatgcc gagaccgatt cctccgttac
     9601 cgccggtgac gatggcgacc ttgcccttca gatcgaacac gcgagcctcc ctcgacgatc
     9661 gatcgaactg gaccgaagtg ggctgccggc ctcgaccagc tctgatcgac tgtaactctt
     9721 acaccagttc cctggaccga cttgaacttc cttcaaccgg cgccgcggct gagcgcgcat
     9781 tcgcgctgca tatcgaaccc gcggcgctgg cacagcggct gcagtcctgc cggactacca
     9841 tgacgcagac cagaccggtt gactcgcatc gcttctgggg tgtcgtgctc gccggcaacc
     9901 tcgacgagcg tcagccccga cgaccggcac gcgccgtccc cgacgagcgt gtccgcgagc
     9961 agcgcgcgct gttccggcag atgctggagc gcgcgacgtg gctcgtcccg gccgagcggc
    10021 tcgtggcggt gctctcccgg gaccacgctg catattacga cagcgacctg cagggcctcg
    10081 ggccgattca cacggtcgtg caaccggcct ggcgaggcag cgcggccggc cttttcctgc
    10141 ctattctgaa gattgccgcc ggcgaccctg acgccacggt cgcgatcttc cccggtggcc
    10201 agccggtgta cggcgatgcg cagctcatga cgtcggtgca caaagcggtt cgggccgtct
    10261 ccgtccgccc cgagcttccc gtcgtcatcg gtgcggctcc acgcggaccc gacctggcac
    10321 aggcgtggat cgaaccagcc gaggccgtcg agggcctcga accgtacgcc gtccgtgccg
    10381 tgcggcggtt cgtccatcgg cccagccggg ccgaggcggc ctcgctgtgg gagggcgacg
    10441 gcctcgtcaa cacgcacgtc gtcatcgcga aggcccgggc gctgatcgag ctcggtggac
    10501 gctacctccc cgacgtcctc gagacgttcg aacccctcgg tagcgcgttc ggaacccccg
    10561 aggaggcctt gctctgcgag gcggtctacg aggagatgcc ctacgcctcg gtggcgcacg
    10621 ccctgttcgt gcgggggcga gacgtcgccg tgctggccgc cccccacgtc aggacgaggc
    10681 tcgaagcgcc agccctcgcg gacgcgctcg cctcctgagg gcccgggccg aatggccctg
    10741 ctgctgaccc gccgccgcgc tcgctctcgt cgaggcggag gcgaggggca ccgtcctgcc
    10801 gctggccgcg gacggacgtc cggccacagc gtaccgggcg ggcgccgggc tcaccgcgct
    10861 cgcgctgctc gcctccgtca cgacgcacga ggcggtgctc gggaggctgc ccttcacgcg
    10921 gagccacgcc gccgccgcag ccgctgtcgc cgggcgcacg ctggctggcg gcgtgctcgt
    10981 gctggccgtg atcgctggcg cctcgggcga caccgcgctc gccctctggg gcgcgctgct
    11041 gggcctcgtc acgcgggacc gaggcggggg cgagtcgccc ggctcacgat cccgccgccg
    11101 gaagggtcag cctgacttcc gtcccacccc cgggcggcgt cgcgaactcg atccggccgc
    11161 cgtgcaagcg ggccacccgc tcgaccgtga cgagccccac gcggggccga tcaccgggcc
    11221 ggtatcccag acggatgagc gcgcgcgggt tggtggtccg caggcccgag ccccagtccc
    11281 gcacgatcag caccgccgag tccccggcgt cacgcgcgtc gatctctggc gcgcgtccgc
    11341 cgtcggcggc cagcgcgtta cggatcaggt gcgcgacggc cagccgcaac aagcgagcgt
    11401 ccccgggcac gcgcggccag ggtccgggcg cgaccacgac cccgcgcagc tcgtgctcgg
    11461 cgagcgcggt ctccacgatc gcgcggaggt cgtgagcctc gcgctcgtgg agctcgaccg
    11521 ccatcacatg gaaggcggcc ggcagcatgg tggcgaggcg gctgacttcc tgggcgatgc
    11581 tctcgaggct ggcgagcgcg gccggctcgc cgacgtccag acgcttgagt agctcgacgt
    11641 gcatctgcat cgcgttgagc ggcccgcgga gatcgtgaag gagccggtcg agatcctcgg
    11701 gcagaccgcc catcaggtga tgcgggccga cttcaccgcc tggatgccgt acttggccag
    11761 cttgttgtag agggtcttga ggctgatgcc gagcagcgcc gccgccttgc gcttgtcctg
    11821 gctcgtctcc tcgagcgctc tgaggatctc ctcgcgctcg agctcgcgga tcgtccggat
    11881 gctgccggtg gccacggcct tggccggctc cgcgccgcgc agcacgttgt cgggcaggtg
    11941 ctcgacgccg atgaggcccg taccgctcag caccaccgcg cgctggatga cgttgcgcag
    12001 ctcgcgtacg ttgccgggcc acgcgtagcg gtccagcgcc ttctcggcct cggcgttgag
    12061 cccgcggacc tggcggttgt cctggcggtt gaactcgtcg atgaacgtct tgccgaggag
    12121 cgggatgtct tccttgcgct cgcgcaaggc cggcaggcgg atggcgaaga cgttgagccg
    12181 gtagaagagg tcctcccgca gctgcccctg ggccatcgcg tcggtgggga cgcgattggt
    12241 ggcggcgatc acccggacgt cgacctggat ctcacccttg ccgccaccca cgcggcggaa
    12301 actgccctcc tggagcaccc ggagcaactt ggcctgggtg gaattgtcca tctcggcgat
    12361 ctcgtccagg aacagcgtgc cgccgtcggc gagctcgaag cagcccagcc ggcgctcggt
    12421 ggcgcccgtg aaggcaccgc gctcgtgccc gaacagctcg ctctcgatca acgtttcggg
    12481 gatggccgcg cagttgatcg ccacgaacgg cttgcttcgc cggttcgaca ggtcgtggat
    12541 cgtccgcgcc accagctcct tgccggtgcc gctctcgccg tagaccagga cgggggcggt
    12601 gctcgtggcc g
//
LOCUS       DATIAZ010000126         5914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_30949, whole genome shotgun sequence.
ACCESSION   DATIAZ010000126 DATIAZ010000000
VERSION     DATIAZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30949"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VLF19_04315"
     CDS             <1..309
                     /locus_tag="VLF19_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HSE92509.1"
                     /translation="FVCTSGETCPTQGDVESYVKWLRGECAKAGLKTEVRINKAGCFS
                     QCGHGPMLVVYPDDVWYAGVQQSDLREILESHILGGMPVERLRYAPGVKGANKTPDAH
                     "
     gene            414..2111
                     /locus_tag="VLF19_04320"
     CDS             414..2111
                     /locus_tag="VLF19_04320"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSE92510.1"
                     /translation="MRLRQSLVPTLREDPAEAEAASHKLMLRAGLVRQLAAGIFVYLP
                     LGQRVIDKITAIIREEMNAIGGQEIAMPVLHPADIWKQSGRWEGIPEMFRLKDRHGRD
                     MCLGMTHEEVVAWMAAREIRSYRDLPQIWYQIQTKERDEARPRSGVLRTREFLMKDSY
                     TLDPDLAALDRSYAAHEGAYRRIFERCGLRFHVVQSDTGMMGGHSAHEFMAPSAAGED
                     EIAMCPRCEYAANVELARAVPVAPEFPAWSREEVATPGAKTIAEVAAFLGIDPRLTIK
                     SLLYVAPQAGPVLVLVRGDHHLHERKLMRAVGEECRPAHPDEVRQCLGAPVGSVGPIG
                     VSVPVVADEALRDGTYVVGANREGVHVRGVAPGRDFECRFADLHTAAAGEGCPTCGAP
                     LAIERVIEIGNIFKLGTKFSDALGATYLDEAGKQQPVVMGSYGIGPARIAAAAIEQLA
                     DTDGIVWPPAIAPFAVHIVVVNVKDAAQTQAAEELYAECARHGIDALLDDRDERPGVK
                     FKDADLLGVPLRVTVGNAFVKEGVVEIRERRTRREHRVPKSGAIEAIRASGVLVAPG"
     gene            2201..2698
                     /gene="rimP"
                     /locus_tag="VLF19_04325"
     CDS             2201..2698
                     /gene="rimP"
                     /locus_tag="VLF19_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HSE92511.1"
                     /translation="MEQDERSIRIEQVVEPVLGGHGLELVDLEWRPHGPRAILRVAVD
                     KPGGVGIADCERVSRELGDVLDVAGLIDAAYDLEVSSPGLDRQLRRERELRWAVGKRV
                     RCWLAGGEEVRGWLAEVGPDRLALDREDGRAELARANVTKVRLDADVPWPRRGEGRDV
                     GERRG"
     gene            2702..4003
                     /gene="nusA"
                     /locus_tag="VLF19_04330"
     CDS             2702..4003
                     /gene="nusA"
                     /locus_tag="VLF19_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSE92512.1"
                     /translation="MNRELLMVVEQIGREKGIDPEILFEALESALLSASRKTLGAAEN
                     VRMEIDRKTGAMKVYCRKKVVEAVTDPKLEIALPEAKALNPEAELDDELEQELPPQEF
                     GRIAAQTAKQVILQRVRDAERDRIYKEFEGKEGQILRAVVHRIEKRNAILEIGKAEAI
                     LPEREQIPGERHNPGDRIRAYVLEVRRTAKGPQITLSRTHPGFLARLFETEIPEIQEG
                     IVVVKAAAREPGERAKVAVASTKRDVDPIGACVGLRGTRIQVISRELRGEKIDIIEWS
                     HDPATFVARALSPAKVSSVTIADDNAEGQAAALVVVPDNQLSLAIGKKGQNARLAAKL
                     TGLRIDIKSESELEAARGAPGAAGDGREALAGLAGVGPEAVEALLAAGLGSPRAIVGA
                     GRDRVAAIAAIGDRAALVVAAAEEWLAAHEGGGTSGESVTA"
     gene            4012..4257
                     /locus_tag="VLF19_04335"
     CDS             4012..4257
                     /locus_tag="VLF19_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HSE92513.1"
                     /translation="MPRRTCVGCRRVRPKPELIRLVRRAGGAVVRDPHGPGRGAYVCD
                     DAACMDRALKGGRLAQAFRTPRETVGVATINDAVSGR"
     gene            4265..>5914
                     /locus_tag="VLF19_04340"
     CDS             4265..>5914
                     /locus_tag="VLF19_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HSE92514.1"
                     /translation="MAATKIKVIELAKELGVTSKDLILAAEEMGRKGVRAMTPLDTTL
                     ANELRVKLGKGRELPEEAKPKRPSRAKVKPEGEDGLAKTVSRLRKKAEPADETGAPIE
                     LRPAATIVKPKPAEKVEGVEAPEPLKPVAEIVVEPQAPLPAPEPVLPPVAKPVVTPPP
                     APAAPPAPPPPPPGPTIKRPEPKIVPFRPLERPQPGPVRPGPRQPAGPFAPPRISQAP
                     SRPAPPPREPRRVEPVREPVAPAAAAVTAPVEIVASRPAASGAGKPAAVEAPPAPPVE
                     IKRETIRVPESVTVAELAEKMRKKSGEVIKGLIELGVMRMVNDLLDPMEAKLVADKFG
                     FDLEIRSVEGEVIDEEEGAEAGSLQLRPPVVTVMGHVDHGKTSLLDAIRKSKVAEKEF
                     GGITQHIGAYQVMTSHGKVTFLDTPGHEAFTAMRARGAQATDIVILVVAADDGVMPQT
                     VEAINHARAANVPIIVAVNKIDKPEADPDRVKRELANHDLVPEEWGGQTIYVPTSAKK
                     LTGVDQLLEMTALQAEILELKANPARAAKGVIIESRLDRGRG"
BASE COUNT          948 a         2004 c         2210 g          752 t
ORIGIN      
        1 ttcgtctgta ccagcggcga gacctgcccc acgcagggcg acgtcgagtc gtacgtgaag
       61 tggctgcgcg gcgagtgcgc caaggccggg ctcaagaccg aggtccggat caacaaggcc
      121 ggctgcttct cgcagtgcgg ccacgggccg atgctggtcg tctatcccga cgatgtctgg
      181 tacgcaggcg tgcagcagtc ggacctccgg gagattctcg aatcgcacat cctcggcggc
      241 atgccggtgg agcgcctccg ctacgcgccc ggcgtcaagg gcgccaacaa gacgccggac
      301 gcccactgat gacatgagcg cccggacctg cccccccgag tccccagcgc cgggcacgtc
      361 catgctgcgc catggcgcgc ctcttcgacc ccacgggcgc ctagactagc cggatgcggc
      421 tgcgccagtc gctcgtcccc acgctccggg aagatcctgc cgaggccgag gcggcgagcc
      481 acaagctcat gctacgggcc ggcctggtgc gccagctcgc cgccggcatc ttcgtctacc
      541 tcccgctcgg gcagcgcgtc atcgacaaga tcaccgcgat catccgcgag gagatgaacg
      601 ccatcggcgg gcaggagatc gcgatgcccg tgctgcaccc ggccgacatc tggaagcagt
      661 ccggacggtg ggaggggatc cccgagatgt tccggttgaa ggatcggcac gggcgggaca
      721 tgtgcctggg gatgacgcac gaggaggtcg tggcctggat ggccgcgcgg gagattcggt
      781 cctaccgaga cctgccgcag atctggtacc agatccagac caaggagcgc gacgaggcgc
      841 gcccgcgctc cggcgtgctg cgcacgcgcg agttcctgat gaaggactcc tacacgctgg
      901 atcccgacct cgcggccctc gaccggtcgt acgcggccca cgagggcgcg tatcgtcgta
      961 tcttcgagcg ctgcgggctg cggttccacg tcgtgcagtc cgacaccggg atgatggggg
     1021 gccacagcgc gcacgagttc atggcgccca gcgcagccgg cgaggacgag atcgcgatgt
     1081 gcccgcgctg cgagtacgcg gccaacgtcg agctggcccg cgcggtgccg gtggcgccag
     1141 agtttccggc gtggagccgc gaggaggtgg ccaccccggg ggcgaagacc atcgccgagg
     1201 tcgccgcgtt cctcgggatc gacccgcgtc tcaccatcaa gtcgctgctc tacgtggcgc
     1261 cgcaggcggg gcccgtgctg gtgctcgtcc gcggtgacca ccacctgcac gagcgcaagc
     1321 tcatgcgtgc ggtcggcgag gagtgccggc cggcgcaccc cgacgaggtc cgccagtgcc
     1381 tcggcgcgcc ggtaggctcc gtgggaccga tcggcgtgag cgtccccgtc gtcgccgacg
     1441 aggccctgcg cgacgggacg tacgtcgtcg gcgccaaccg cgagggcgtc cacgtgcgcg
     1501 gtgtcgcgcc gggccgcgac ttcgagtgcc gcttcgcgga cctccacacc gcggcggccg
     1561 gtgaggggtg cccgacgtgc ggagcgccgc tggccatcga gcgggtcatc gagatcggaa
     1621 acatcttcaa gctggggacg aagttctccg atgccctggg cgccacatat ctcgacgagg
     1681 ctggcaagca acagccggtg gtgatgggca gctacgggat cggacccgcg cggatcgcgg
     1741 ccgcggccat cgagcagctc gccgacaccg acggcatcgt gtggccgccg gcgatcgccc
     1801 cgttcgcggt ccacatcgtc gtcgtcaacg tgaaggacgc cgcgcagaca caggccgccg
     1861 aggagctcta cgccgagtgc gcccggcacg gcatcgacgc cctgctggac gaccgcgacg
     1921 agcggccggg cgtgaagttc aaggacgccg atctcctcgg cgtgccgctc cgggtcaccg
     1981 ttggcaacgc cttcgtcaag gaaggcgtcg tggagatccg cgagcgccgc acgcgccgcg
     2041 agcaccgcgt gccgaagtcc ggcgcgatcg aggcgatccg cgcgagcggg gtcctcgtgg
     2101 cgccgggcta gcgtcggcca agcttgcctc ggtaggtcgt acgtgctaaa gtcttgaaaa
     2161 actgggcgtt gcccagtttt ttactttcac gggtgtggtg atggagcagg acgagcggag
     2221 catccggatc gagcaggtcg tcgagccggt gctcggggga cacgggctcg agctggtcga
     2281 tctcgagtgg cgaccacacg ggccgcgcgc gatcctgcgc gtggcggtgg acaagccggg
     2341 cggcgtgggg atcgccgact gcgagcgcgt gagccgcgag ctcggcgacg tgctcgacgt
     2401 cgcgggcctg atcgacgcgg cctacgacct cgaggtctcg tcgccgggcc tggaccggca
     2461 gctccggcgc gaacgcgagc tccgctgggc cgtcggcaag cgcgtgaggt gctggctcgc
     2521 cggtggtgag gaggtccgtg gatggctcgc cgaggtcggc ccggatcggc tggcgctcga
     2581 ccgtgaggac ggccgcgcgg agctcgcgcg cgcgaacgtg acgaaggtcc ggctggatgc
     2641 ggatgtcccg tggccccgcc gcggcgaggg ccgcgacgtg ggcgagcggc gtgggtaggg
     2701 catgaacaga gagttgctga tggtcgtcga gcagatcgga cgcgagaagg ggatcgatcc
     2761 cgagatcctc ttcgaggcgc tggagtctgc cttgctgtcg gcgtcccgca agacgctcgg
     2821 cgcggccgag aacgtgcgga tggagatcga tcgcaagacg ggtgcgatga aggtctactg
     2881 ccgtaagaag gtggtggaag cggtcacgga ccccaagctc gagatcgcgc tgcccgaggc
     2941 caaggccctc aaccccgagg ccgagctgga cgacgagctc gagcaggagc tgccgccgca
     3001 agagttcggg cgcatcgcag cgcaaacggc caagcaggtg atcctgcagc gggtgcgcga
     3061 cgcggagcgc gaccggatct acaaggagtt cgagggcaag gaagggcaga tcctgcgcgc
     3121 ggtcgtgcac cggatcgaga agcggaacgc gatcctggag atcggcaagg ccgaggcgat
     3181 cctgcccgag cgggagcaga tcccgggcga gcgccacaat cccggcgacc gcatccgggc
     3241 ctacgtgctc gaggtgcgcc ggacggcgaa ggggccgcag atcacgctgt cacggacgca
     3301 tcccggcttc ctggcgcggc tgttcgagac ggagatcccc gagatccagg aggggatcgt
     3361 cgtcgtcaag gccgccgcgc gggagcccgg cgagcgagcc aaggtcgcgg tggcgtccac
     3421 caagcgcgac gtcgacccga tcggggcgtg cgtcgggctg cggggcaccc ggatccaggt
     3481 catcagccga gagctgcggg gcgagaagat cgacatcatc gagtggtcgc acgacccggc
     3541 cacgttcgtg gcacgcgcgc tgtcgccggc caaggtctcg tcggtgacga tcgccgacga
     3601 caacgccgag ggacaggcgg ccgcgctggt cgtggtgccg gacaaccagc tgtcgctggc
     3661 gatcggcaag aagggacaga acgcgcgact ggcggccaag ctcaccggct tgcggatcga
     3721 catcaagagc gagtccgagc tggaggccgc gcgcggtgcg cccggcgccg ccggcgacgg
     3781 ccgcgaggcg ctcgccgggc tggccggcgt cggccccgag gcggtcgagg cgctcctggc
     3841 cgccgggctc ggctcgccgc gcgcgatcgt cggcgccggg cgcgaccggg tggccgccat
     3901 cgccgcgatc ggcgaccgcg ccgccctggt cgtcgcggcg gccgaggaat ggttggcggc
     3961 gcacgagggc gggggcacct ccggcgagtc cgtgaccgca tgatacgcga ggtgccgaga
     4021 cgcacatgcg tcggctgccg gcgcgtccgg ccgaagccgg agttgatccg gctggtgcgg
     4081 cgggccggcg gcgcggtggt tcgcgatccc cacggacccg gccgcggcgc ctatgtgtgc
     4141 gatgatgcgg cgtgcatgga ccgcgcgctg aagggcggga ggctcgcgca ggcgtttcgg
     4201 acgccgcggg agacggtggg cgtggcgacg atcaacgacg cagtgtcggg gaggtgaggg
     4261 gaagttggca gcgacgaaga taaaggtcat cgagctcgcg aaagagcttg gcgtcaccag
     4321 taaggatctc atcctggccg ccgaggagat gggcaggaag ggtgtccgcg ccatgacccc
     4381 cctcgacacg acgctcgcca atgagctgcg cgtgaagctc ggcaagggac gcgaactccc
     4441 cgaggaggcc aagcccaagc ggccctcgcg cgccaaggtc aagcccgagg gcgaggatgg
     4501 cctcgcgaag acggtgagcc ggctgcgcaa gaaggccgag cccgcggacg agaccggcgc
     4561 gcccatcgag ctcaggccgg cagcgacgat cgtcaagccg aagcccgcgg agaaggtcga
     4621 aggcgtcgag gcacccgagc ccctcaagcc cgtcgcggag attgtggtcg agccgcaggc
     4681 acccctgccc gcaccggagc ccgtgctgcc gcccgtggcc aagccggtcg tgacgcctcc
     4741 accggcgccg gcggctccgc ccgcgccacc gcctccgccg cccgggccca cgatcaagag
     4801 gcccgagccg aagatcgtcc cgttccggcc gctcgagcga ccgcagcccg ggccggtgcg
     4861 acccgggcct cggcagccgg ccgggccctt tgcgccaccg cgcatcagcc aggcgccgtc
     4921 gcgtccggcg ccgccaccgc gcgagccgcg gcgcgtcgag cccgtacgtg agcccgtggc
     4981 gccggccgcg gccgcggtca cggcgcccgt ggaaatcgtc gcctcccggc ccgcggcctc
     5041 aggggccgga aagccggccg cggtcgaggc gcctccggcg ccgcccgtgg agatcaagcg
     5101 ggagacgatc cgcgtcccgg agtcggtgac cgtggccgag ctggccgaga agatgcggaa
     5161 gaagtcgggg gaggtcatca agggtctcat cgagctgggc gtgatgcgca tggtgaacga
     5221 cctgctcgac ccgatggagg ccaagctcgt cgccgacaag ttcggcttcg acctcgagat
     5281 ccggtcggtc gagggcgagg tgatcgacga ggaggagggc gccgaggccg gtagcctcca
     5341 gctccggccg cccgtggtga cggtcatggg tcacgtcgac cacggcaaga cctcgctgct
     5401 cgacgcgatc cggaagtcga aggtcgccga gaaggagttc ggcggcatca cccagcacat
     5461 cggcgcctac caggtcatga cctcgcacgg caaggtgacc ttcctcgaca caccgggcca
     5521 cgaggcgttc accgccatgc gcgcgcgcgg cgcccaggcc accgacatcg tcatcctggt
     5581 cgtcgccgcc gacgacgggg tgatgccgca gaccgtggag gccatcaacc acgcgcgtgc
     5641 ggcgaacgtg cccatcatcg tcgccgtcaa caagatcgac aagcccgagg ccgaccccga
     5701 ccgcgtgaag cgggagctgg ccaaccacga cctcgtcccc gaggaatggg gggggcagac
     5761 gatctacgtg ccgacctcgg ccaagaagct caccggcgtc gatcagctcc tcgagatgac
     5821 ggcgctgcag gccgagatcc tcgagctcaa ggccaacccg gcgcgtgccg ccaagggcgt
     5881 gatcatcgag agccgcctgg accgaggccg cggc
//
LOCUS       DATIAZ010000127         5848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1477949, whole genome shotgun sequence.
ACCESSION   DATIAZ010000127 DATIAZ010000000
VERSION     DATIAZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477949"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VLF19_04345"
     CDS             <1..913
                     /locus_tag="VLF19_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92515.1"
                     /translation="TLRIETVPVDLVAVVEAAVGSLLPAAGARGVTVERTLDAAAGPV
                     LGDTGRLQQIVGNLLSNAVKFASEGGRVSVALAREDDHAVIRVADSGPGIDPAVLPHV
                     FDRFQRGGGPPGTGHGGLGLGLAIAHHLVALHGGTLEAAPAGPLGGALFTVRLHCRSS
                     EVPPIAGAEGRDATLPSLAGTRVLVVEDEADTRAFLTATLERAGGAVAAASCADEALA
                     VLDAFRPDVLVADLRMPGKDGYALIREVRLLDAHHGHRLPALAVTAYAGPDEAERARA
                     AGFDRYVSKPLEPAALIRAIVDALDGA"
     gene            complement(1060..2223)
                     /locus_tag="VLF19_04350"
     CDS             complement(1060..2223)
                     /locus_tag="VLF19_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92516.1"
                     /translation="MSRWIAAGAAFVVSLDSMVNIAFPAMAASFDLPAERVRWVIIGY
                     VLTYALTAFAGGTAGDRAGHGRVLAAGLALSAAGYVACGLAPAFGWLVVGRVVQGIGG
                     GLVYGTAPALVTLGVEVSRRGRALGFLAASMGLAFSMGPVLAGALVERFGWAAVFHVR
                     APLAVALLVWAVAGLPAARMRTGAPRLAVLDVVRAPVLGLGALAFAGNAGIFAIWLLA
                     PFYLLERRGLDAVTGGALFMLTPLGTTLAAPLGGWLADRVGPRAPAAAGLALEGAALA
                     TLSRAEPATPVAVVALALFAAGAGLGAFQVPNMAAVMGAFPAGQQGAAGGFAFLARTL
                     GTVAGVALLAEVFARRRGAAGFDNAFASAFLAASALVAAAAIAATLTGRRRAD"
     gene            2316..3047
                     /locus_tag="VLF19_04355"
     CDS             2316..3047
                     /locus_tag="VLF19_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSE92517.1"
                     /translation="MPDLTGWVILLVGALVGSVVGGIAGFGAGIILLPLLAWTFGVRA
                     AAPLLTVTMLLGNLSRIWWSRTEVDHAVTIRFLAGAVPATVVGAVFYAAAPVESLRWV
                     MGAFLIAAVPVRRLLLTRWVGVRLRHFPALGAAIGVLSGIVVATGPVMTPFLLAYGLR
                     RGAYIATEAVCALGMHAARAATFTRYSLLSWEALVIGGALGSVMFAGSWLGRRLLDRM
                     TDRVFLALIEALLILMGLQFLLVSR"
     gene            3059..3454
                     /locus_tag="VLF19_04360"
     CDS             3059..3454
                     /locus_tag="VLF19_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSE92518.1"
                     /translation="MTPSPGATAEVALPVTPDRTAHAMGNTGVHVLATPFLVGLLENA
                     CAAVIHPHLPPGAGTVGTMVEMRHLAATPVGMTARAKATLLETDGRRYVFSVEAWDER
                     DKIAEGRHERFVVADRAKFLERVMQKARG"
     gene            3454..3909
                     /locus_tag="VLF19_04365"
     CDS             3454..3909
                     /locus_tag="VLF19_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HSE92519.1"
                     /translation="MPLRLNKDVLGKEYPPFAVTVERGRIKDFARAIGDANPFYLDDD
                     VGRASPWGDVIAPPTFAVSFRDESADSNALLRDLGVDISRLLHGEQEFELHGQLRPGE
                     TYLCRTKVVDIYEKTGRSGPMAFVVRETTVTDRHDDIVVTMRQVTVVRL"
     gene            3917..4354
                     /locus_tag="VLF19_04370"
     CDS             3917..4354
                     /locus_tag="VLF19_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HSE92520.1"
                     /translation="MRYGKVYFEDVGVGAEIPPLVKEPIRQIQLTRYAGASGDFNPIH
                     QDDEFARAAGMGGIFAHGMLSMGFVAQALTDWAGAGTVRKLGVRFAGLVRLKDVITCR
                     GRVLAKSSKDDEHHVELEVWAENQKGEKVVAGKAAVALPSRVP"
     gene            complement(4401..>5848)
                     /locus_tag="VLF19_04375"
     CDS             complement(4401..>5848)
                     /locus_tag="VLF19_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSE92521.1"
                     /translation="RLNIVILDACRDNPFARSFRSTSKGLASIDAPSGTMIAYATAPG
                     RVARDGTGSNGLYTGELVKTISAPGVKVEDMFKKVRQSVSAQTRGEQVPWESSSLVGD
                     FIFVLPAEGERPATTSPPVTPPAAPPPRAAPPAIATRPPDVREVPRPAEVVGALSLSA
                     SVDGVEVSVGDRRIGEARAGIPLVVSLRPGIHRVTARKPGHREWQRDVQIAANQSASM
                     TIDIEPLRSDGPGVIRGEDGAEMVLIPAGEFWMGDDAEERTRPRRRIHLDAYYLDKLE
                     VTNAQFGRFVSAGGYDRQAAWSEAGWQWRASQSSVIGHPKFWTDAKWNQPTQPVVGVS
                     WHEADAFCRFVGKRLPTEAEWEKAARGTEGRRYPWGDAVEPGRANTDESRTGRTAAVG
                     SHPSGASPYGVHDLVGNAAEWVADWYAKGYYRVGPERNPPGPAAGGDKAVRGGSWDNK
                     LKDLPSAARRDERPSERGRRIGFRCAADPPR"
BASE COUNT          773 a         2202 c         2078 g          795 t
ORIGIN      
        1 cacgctccgc atcgagacgg tgccggtcga cctcgtcgcc gtcgtcgagg ccgccgtcgg
       61 gagcctgctg ccggccgccg gggcccgcgg cgtcaccgtg gagcgcacgc tcgacgccgc
      121 cgcggggccc gtgctgggcg acaccggccg gctccagcag atcgtcggga acctcttgtc
      181 caacgccgtg aagttcgcgt cggagggcgg gcgggtctcc gtcgcgctcg ctcgcgagga
      241 cgatcacgcc gtcatccgcg tcgccgactc cggcccgggt atcgaccctg cggtcctgcc
      301 ccacgtcttc gatcggttcc agcggggcgg cgggccgccg ggcaccggtc acggggggct
      361 cgggctcggg ctggcgatcg cccatcacct cgtcgccctg cacggcggga cgctcgaggc
      421 agcgccggcc ggaccgctcg gcggagcact cttcaccgtg cgcctgcact gccggtcgag
      481 cgaggtgccg cccatcgcgg gcgccgaggg acgcgacgcg acgctgccct cgctcgcggg
      541 cacgcgcgtc ctggtcgtgg aggacgaggc ggatacgcgc gccttcctca cggccacgct
      601 cgagcgggcc ggtggggcgg tcgccgcggc cagctgtgcc gacgaggcgc tcgccgtcct
      661 ggatgcgttc aggcccgacg tcctcgtcgc ggatctcagg atgccgggca aggacgggta
      721 tgcgctgatc cgcgaggtcc gccttctcga cgcgcatcac ggtcatcgac ttcccgcgct
      781 cgccgtcacc gcatacgcgg gtcccgacga ggccgagcgc gcgcgcgccg ccggcttcga
      841 ccgctacgtg agcaagcctc tcgagcccgc tgcgctgatc cgggccatcg tcgacgcgct
      901 cgacggggcc tgacgacgcc gcgtcgccga ggacaccgcc ggcgccgcca gcgagcggcg
      961 ccgggccaac cgccgccctt cggagtcgcc cggcggaggc gtactcggcc gacggtgatc
     1021 ggggccgcgg ccgcgggaag aacgctggct cggcggcggt cagtcggccc ggcgtcgtcc
     1081 cgtgagggtg gcggcgatgg cggcggcggc gacgagggcg gaggcggcga gaaaggcgct
     1141 cgcgaaggca ttgtcgaagc cggcggcacc gcgacgccgc gcgaagacct cggcgagcag
     1201 ggcgaccccg gccaccgtgc cgagggtgcg cgcgaggaag gcaaaaccgc cggcggcgcc
     1261 ctgctggccc gcggggaagg cgcccatcac ggcggccatg ttcggcacct ggaacgcgcc
     1321 caggcctgcg cctgccgcga acagcgcaag cgccacgacg gccaccggcg tcgccggctc
     1381 cgcgcgcgag agcgtcgcca gcgccgcgcc ctcgagcgcc agccccgcgg cggccggcgc
     1441 acggggaccc acgcggtccg ccagccatcc cccgagcggt gctgccagcg tggtacccag
     1501 cggcgtcagc atgaagagcg cgccgccggt gacggcatcg aggccgcggc gctcgaggag
     1561 atagaagggc gccaggagcc agatcgcgaa gatgcccgcg ttccccgcga acgccagcgc
     1621 gccgaggccc agcacgggcg cgcggaccac gtccagcacc gcgaggcgtg gggcaccggt
     1681 gcgcatccgc gccgccggca gtcccgccac ggcccagacg aggagggcca ccgccagcgg
     1741 ggcgcggacg tggaagaccg ccgcccatcc gaaccgctcc acgagcgcgc cggccagcac
     1801 cggacccatg ctgaacgcga gccccatcga cgcggcgagg aagccgaggg cgcgcccgcg
     1861 ccgcgagacc tcgacgccga gcgtgacgag cgccggcgcc gtgccgtaga cgagaccgcc
     1921 acctatcccc tggaccacgc gcccgaccac gagccagccg aacgccggcg cgaggccaca
     1981 cgcgacgtag cccgctgcgc tgagcgcgag cccggcggcg aggacgcggc cgtggccggc
     2041 gcggtcgccg gcggtgccgc cggcgaaggc cgtgagcgcg taggtgagga cgtagccgat
     2101 gatcacccag cggacgcgct ccgccgggag atcgaaggac gcggccatgg cggggaacgc
     2161 gatgttgacc atcgaatcga gcgacaccac gaaggcggcg ccggccgcga tccaacggct
     2221 cacgtcgagt atcatggcac ggatggatca tccgatggcc gtcggggcgg ctttgccgag
     2281 cctccgccgc ccgacggtcg ggcggacaat ccccgttgcc tgatctcacg gggtgggtga
     2341 tcctgctcgt cggcgcgctc gtcggttccg tcgtcggcgg catcgcgggc ttcggcgccg
     2401 gcatcatcct gctcccgctc ctcgcatgga cgttcggcgt ccgcgccgcc gccccgctgc
     2461 tcacggtgac gatgctgctc ggcaatctct cccgcatctg gtggagccgc accgaggtcg
     2521 accacgccgt caccatccgt ttcctggccg gtgccgtgcc ggccacggtt gtcggcgccg
     2581 tgttctacgc cgcggcgccc gtcgagtcgc tgcggtgggt catgggcgcg ttcctcatcg
     2641 ccgcggtgcc ggtccggcgg ctcctcctca cgcgctgggt cggtgtccgc ctgcggcact
     2701 tccccgcgct cggcgcggcg atcggcgtgc tctccgggat cgtcgtggcg accgggcccg
     2761 tgatgacgcc gttcctcctc gcctacgggc tgcgccgtgg cgcgtacatc gccaccgagg
     2821 cggtctgcgc gctcggcatg cacgccgcac gtgccgcgac gttcacgcgc tactccctgc
     2881 tcagctggga agccctcgtc atcggcggcg cgctgggctc ggtgatgttc gcgggctcct
     2941 ggctcgggcg ccgcttgctc gaccgcatga ccgaccgggt cttcctggcg ctcatcgagg
     3001 cgctgctgat cctgatgggc ttgcagttcc tcctcgtgtc gcggtaaaac ggcacgccat
     3061 gaccccgagc ccgggcgcca ccgcggaagt cgcgcttccc gtcacgcccg accgcaccgc
     3121 ccacgccatg ggcaacacgg gcgtccacgt gctggccacg ccgttcctgg tcggactgct
     3181 cgagaacgcc tgcgcggcgg tgatccaccc tcacctgccg ccgggcgcag gcacggtcgg
     3241 caccatggtc gagatgcgcc acctcgcggc cacgccggtc gggatgaccg cgcgcgcgaa
     3301 ggccaccctg ctcgagaccg acggccgccg ctacgtcttc agcgtcgagg cctgggacga
     3361 gcgggacaag atcgccgagg gacggcacga gcgattcgtg gtggccgacc gcgcgaagtt
     3421 cctcgagcgc gtcatgcaga aggcccgagg ctgatgccgc tccggctgaa caaggacgtc
     3481 ctcggcaagg agtatccgcc gttcgccgtg accgtggagc ggggccgcat caaggacttc
     3541 gcccgcgcca tcggcgatgc gaaccccttc tacctggacg acgacgtcgg ccgcgcatcc
     3601 ccctggggcg acgtgatcgc cccgccgacg ttcgccgtgt ccttccgcga cgagtcggcg
     3661 gactccaacg ccctgctccg cgacctgggc gtggacatct cccggttgct gcacggcgag
     3721 caggagttcg agctgcacgg ccagctccgt cccggcgaga cgtacctttg ccggaccaag
     3781 gtcgtggaca tctacgagaa gaccggccgc agcgggccga tggcgttcgt cgtgcgcgag
     3841 acgaccgtga ccgaccgcca cgacgacatc gtggtgacga tgcgccaggt caccgtcgtg
     3901 cggctgtgag ggtcacgtga ggtacgggaa ggtctacttc gaggacgtcg gggtcggtgc
     3961 cgagatcccg ccgctcgtca aggagccgat ccggcagatc cagctcaccc gctacgccgg
     4021 cgcctcgggc gacttcaatc cgatccacca ggacgacgag ttcgccagag ccgccggcat
     4081 gggcggcatc ttcgcccacg gcatgctctc gatgggcttc gtggcccagg cgctcaccga
     4141 ctgggccggc gcgggcaccg tgcgcaagct cggcgtccgc tttgccgggc tcgtgcgtct
     4201 caaggacgtc atcacctgtc gtggccgcgt cctcgcgaag tcgtccaagg acgacgagca
     4261 ccacgtcgag ctcgaagtct gggcggagaa ccagaagggc gagaaggtcg tcgccggcaa
     4321 ggccgccgtc gccctgccct cccgcgtgcc gtgacgcgct ggaccgacga aggatcgcca
     4381 gtcggatcgc ggccgagacg tcagcgcggc gggtcggcgg cgcagcggaa cccgatcctg
     4441 cggccgcgct cgctcggcct ctcgtcccgg cgcgccgccg acggcagatc cttgagcttg
     4501 ttgtcccacg atccgccgcg taccgccttg tccccgccgg cggccggccc cggggggttt
     4561 cgctcggggc cgacccggta gtaccccttg gcgtaccagt cggcgaccca ctccgcggcg
     4621 ttccccacca ggtcgtggac accgtacggg ctcgcccccg atgggtgcga gccgacggcc
     4681 gcggtccggc cggtccggct ctcgtcggtg ttggcccggc cgggctccac cgcgtcgccc
     4741 cacggatatc tccgcccttc cgtgcctcga gccgcctttt cccactcggc ctcggtcggc
     4801 agccgcttgc cgacgaaccg acagaaggca tcggcctcgt gccacgacac gcccaccacg
     4861 ggctgggtgg gttgattcca cttcgcgtcg gtccagaact tcgggtgccc gatcacggac
     4921 gactgcgacg cccgccactg ccagcccgcc tcgctccacg ccgcctgacg gtcgtagccg
     4981 cccgcgctga cgaaccggcc gaactgggcg ttggtcacct ccagcttgtc gaggtagtac
     5041 gcgtcgaggt gtatccggcg acgcggtctg gtccgctcct cggcgtcgtc gcccatccag
     5101 aattccccgg ccgggatcag gaccatctcc gcgccgtcct cgccccggat caccccgggg
     5161 ccgtcgctcc tcagcggctc gatgtcgatc gtcatgctgg cgctctggtt ggcggcgatc
     5221 tgcacgtccc gttgccactc ccggtggccc ggcttgcgcg cggtgacccg gtgaatgccg
     5281 ggccgaagac tgacgaccag cggtatcccg gccctcgcct cgccgatgcg ccggtccccg
     5341 accgagacct ccacgccgtc caccgacgcg ctcagtgaca gcgcgccgac gacctcggcg
     5401 ggacggggaa cctcgcggac gtcgggcggc ctcgtggcga tcgccggagg ggccgcacga
     5461 ggagggggcg ccgccggcgg cgtcaccggc ggcgacgttg tcgccgggcg ctcgccctcg
     5521 gcgggcagga cgaagatgaa gtcacccacc agcgacgacg actcccaggg tacttgttcc
     5581 ccgcgcgtct gggccgacac ggactggcgg accttcttga acatgtcctc gaccttcacg
     5641 cccggcgccg agatcgtctt caccagctct cccgtgtaga gcccgttgct accggtgccg
     5701 tcgcgcgcca ctcgccccgg cgccgtcgcg taggcgatca tcgtccccga cggggcgtcg
     5761 atcgacgcca gccctttgga cgtgctgcgg aagctgcggg cgaacgggtt gtcgcggcag
     5821 gcgtcgagga tgacgatgtt gagccggt
//
LOCUS       DATIAZ010000128        24223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1742259, whole genome shotgun sequence.
ACCESSION   DATIAZ010000128 DATIAZ010000000
VERSION     DATIAZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 24223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24223
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742259"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1073
                     /locus_tag="VLF19_04380"
     CDS             273..1073
                     /locus_tag="VLF19_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92522.1"
                     /translation="MREFDRDPVARTSLLWLFPLVGGLLPFAATIAAFALYNTLFTGH
                     VCNPFVDGCVSISRTARYGVANHLFRILMLPAAALQALTWVLCSYWLSDIGAVRDRAL
                     RILPWLGVASALLLGVYATLLGTPGQPYSLARRYGIPTYFVGTYLCMVITATIISRFE
                     GPTSTTVPARFQRLLFLLALFMTGAGLVQIGVRPFIVEKSLRGRLTNVLEWNTGLAFT
                     VFFTVLAYLWYHTRFSVQPATAGRRTLAAGSAGGEPAGELLQVRRRTQ"
     gene            complement(1067..2476)
                     /locus_tag="VLF19_04385"
     CDS             complement(1067..2476)
                     /locus_tag="VLF19_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HSE92523.1"
                     /translation="MKRELIAALLIGVLAVPAEVPAAPLTVTAETQRNIMVTVYNGNL
                     GLVKDVREARLPPGTTEVQFMDVAALIDPTTVHLKSLTDPAGLKILEQNYEYDLLSSQ
                     KLMEKYVGKKVRLYQSDGTFHDATLLSTNGPVYEINGQIHLGHTGRLILPALPDNLVL
                     RPTLAWLLRNQTATQQRVEASYLTGGITWKADYVLVVNAADTRGDLTGWVTIDNKSGA
                     TYRSATLKLVAGDVNRARDPRREGRVLETAAKAATAADARRDFASEGFFEYHLYTLDG
                     RTTIKDNQTKQLSLLAASDVGVKKHLIYYGAQDYYRNQYGVPVSHQKIGVYLELRNSK
                     DNRLGVPLPKGKLRVYKADTAGSQQFVGEDWIEHTPKDERVQIKVGDAFDVVGERTQR
                     DFRKIASHVWEVEWEISLRNHKKEAQTVTVIEPVPGDWQVLHATHAAEKIEAHTLRFA
                     VPVAAEGAARLTYRVRVRY"
     gene            complement(2631..3797)
                     /locus_tag="VLF19_04390"
     CDS             complement(2631..3797)
                     /locus_tag="VLF19_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSE92524.1"
                     /translation="MTCHDARELFSALLDDALTAEDRARLDGHLATCGECGRELEGFR
                     GAVALVRGLPPDRAPAGFVDRLVAAARPEPWTTRLARRLFLPWTVKLPLEAAALLLVG
                     GFAVLVFRGTEEQERAARVDEPRARIEQPAPAPGVGGRESELPGPAPGVASRPAEPPA
                     IRQDQSWGARDAAGDLAKDPQVSRRVPTQAPAPHVPAPSRAPEAPKASASPQAPGARQ
                     EAGASGEAADGVSAPRPEAPPRREAPLPLGSDRRQKSARAQESTGQAAPPGTQADGTA
                     RDSTPHEDVARDEIAGRLGGAAGSAAVADAGGTLLVADARAAAAKIEDIARGLGGSVA
                     ARREDGEALLLEVAVPRQRYPDLVRELTSIGQWRPRAEPPALPPSVHVVIRLTR"
     gene            complement(3794..4351)
                     /locus_tag="VLF19_04395"
     CDS             complement(3794..4351)
                     /locus_tag="VLF19_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSE92525.1"
                     /translation="MRGPVSDPPGLVDRLRAGEPAAFEDLVRTYQHRVFGVAARMLGS
                     AAEAEEIAQEVFLRVHRSVGDFRGQARLSTWLYAITSRLCLNRLASGARRRERADEEA
                     LREAPSGEADGAAALERGELETALRQAIAELPEERRIVVVLRDVEGLSYDEIAAALDL
                     EPGTVRSRLHRARTDLKAKLERFLP"
     gene            4639..5913
                     /locus_tag="VLF19_04400"
     CDS             4639..5913
                     /locus_tag="VLF19_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSE92526.1"
                     /translation="MRAATLLVCYPGDTGHEDQPVDAAAAMTGALPLEGVRVLELGHI
                     VAGPSAGLLLADLGADVVKVERPGAGDQTRGMPAGNAANFHFLNRNKRSVALDLKGSA
                     EGRELFLRLVKTSDVVIDNFAYGAVEALGLGYDVLARANPGIVYLALRGFLPGPYEAR
                     PFLDELAQMSAGLAFMTGPRGQPMRAGASIVDVGAAAYGVIAVLAALQQRARTGVGQK
                     ITSGLYETTVFWVGQWLANFGATGEPSVPMPEIRQGTRMGWGIYQLFTAADGEQVFIG
                     ITSNAHWERFCREFALDDLLADERLDDNAKRVAARGWLPGRIAEEMRRYPSAELAERL
                     ERARVPFAPLRRPDQLVDDPHLNVSGQFVDTPLPGRGLAKLPKMPVRSTAFEFDLRRP
                     APRLGEHTREVLEEVGLSKDEIDALASRRVIG"
     gene            5910..6893
                     /locus_tag="VLF19_04405"
     CDS             5910..6893
                     /locus_tag="VLF19_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HSE92527.1"
                     /translation="MSDIPRSVKLKEVGPREGFQFEKGPISLADKISLVDALSDTGVK
                     TIEFTSFVSPRWVPQMADAEQMVAGIKRAPGVAYEAIWLNEAGLERAARLRESLALRP
                     MFSVAGSDTFMRKNTNRSVDERLDELPGWAERYRALGMDWLDVGVMAAFGCNYEGDVE
                     PRHVVTVLQRVEQRADACGSRLGGIGLADTMGWANPLQIRRLVGAVRERWPDAPVRLH
                     LHDTRALAIANVVAALEMGIDCFDSAVAGMGGCPFAAHKGAAGNVTTEDVVFLCQEMG
                     IDTGIDLDAMIAAGQLAERVVGHPLPGKLKSGGNLATYRARARAAGAPAAV"
     gene            7182..8396
                     /locus_tag="VLF19_04410"
     CDS             7182..8396
                     /locus_tag="VLF19_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92528.1"
                     /translation="MTSHDTRNMRFLSQHPLDGFGNVGEGMAVQLTRDRRRILWLAHE
                     SAPKNVTAVDVTNPKKPAVVLQTDLPHERMRSNNLDVVGDLLVIAYQTSAPGLTPAGF
                     EILDVADPAKPKKVGFFDASGPTSRGVHHLWFVDGSYVHCASGAADFKPRNPRDDQFY
                     RIVDIRQPSKPVEVGRWWLPGTRDGDTEPPPPRHPKFDAGYRAHNTNVYPQRPDRAYV
                     GYLDGGAIVLDISDMGKPRMVARWDYQPPMPGFCHTVLPLFEPGLLVVSDESTKDGAA
                     DWPKLVWVLDNHVETKPVPIATCPLPPLKKFAKRGGRFGAHNLHENRPGPGTWHSEKV
                     VVGTFFNGGVRAFDLSDPFRPAEVAYAVPPAPRKSPKKAVQLNDVFVDDRGVLFTVDR
                     MIGGLYAYELKV"
     gene            8434..9969
                     /locus_tag="VLF19_04415"
     CDS             8434..9969
                     /locus_tag="VLF19_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92529.1"
                     /translation="MKRLSRRDFLRTTAASTLAGVGAWGPVHAQPSKKTLVYAKNEDI
                     GQLNPYFVNHGIFEALNNHIQEPLVRFDYEKRAWEPVLAESWTTEERGRTWVFRLREA
                     KFHSGEPFTADDVKYTFEHVLKEKSAGPIKANLFMIDTITVRDPRTVAIQCKGPTPGM
                     LSYIQSRPSVMSRALGEKVGFDEAHKKMVGTGSYRFVEYARDEHLIMERNPDYWGEKA
                     RIERIVYRPIPDNTARVTALRAGQVDAMAQVAPFDIGKLEQVPDVKLYSVRAARIHML
                     FMNPITPPLQNKLVRLAIHHGVDVDTIVKTVLEGRAFRLSQLCGPSQVMTYDPDLKPL
                     PYDPERARKLLAQAGYPNGVDLDFHDYPAYAEYKPMGLAVAEQLKKIGVRVSIKPLET
                     SVFRRKWFAGDLSFYFIPYGNVAEDASAFLKAYFRSDQDKRSRYKNPEADALFDQQEA
                     ELDPKRRQALNRRLMRLLQEDSPAVPFYNPEYVVAARKHVQIPAGIPTGGEYVWFWKM
                     DVA"
     gene            9982..10998
                     /locus_tag="VLF19_04420"
     CDS             9982..10998
                     /locus_tag="VLF19_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92530.1"
                     /translation="MGRYLATRLLASLLNLVLVTVLVFVMLHVAPGDPADYLVGPDAT
                     EADRAEARRLWGLDRPLPVQYLAYAGNALRGDLGTSLYFQEPVAQLIRKRAAATLELG
                     ALAVAIVLGVSFVLGVTAGARPHGLLDQTVMMLAVCGVAMPAFWLALLLILVLGGALQ
                     LLPVAGRLSYGTGLASITGFHLVDSVLTANGAAFWDSLRHLVLPAAALAGPGIALQSR
                     LLRSNLLEVMGEDYVRTARAKGLGEPAVIGRHAVWNALTPVLSSWGVLFVFLLSGSVI
                     VETIFAWPGLGRTAIQAINNRDYPLVQGVVLVFTSIVVVANFAVDALCAWIDPRVHYG
                     RGPS"
     gene            10995..11930
                     /locus_tag="VLF19_04425"
     CDS             10995..11930
                     /locus_tag="VLF19_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92531.1"
                     /translation="MSERSAVAERAGVDARRDVAAPTTVAARAGPWHTRPGRLRCAVG
                     GAVVVLLLAVAALAPLLAPASPDSQDLLRRLTPPAWMPGGSAANVLGTDHLGRDILSR
                     IVHGARISAIVGVVAVLVSGVLGTTLGLVAGYYGARVDLVVMKLVELFLTIPFVLLAL
                     VVMALFGQGLAILIVVLCLNRWIHYARVVRGDVLSLREREFVAGARALGASNARIIAR
                     HVLPNTVASLSVVTTFSLATIILIEASLSFLGLGVPPTTPTWGGMLNESRSYMMPSWW
                     TSVFPGLAIFVTVLGVNLLGDGVRDAFDPRLKKKA"
     gene            11967..13022
                     /locus_tag="VLF19_04430"
     CDS             11967..13022
                     /locus_tag="VLF19_04430"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE92532.1"
                     /translation="MPDAILTQVIKSWKPRFVNNGIDMNDYQWTVEPLEKWEQWYDAW
                     MALGRKHEDMAFEAEKAGRAVTAGEAYYRAALAYHFGVFCWVDDLDQYGVGAAKRVSC
                     YGKALATLDPPGERVEFPLERITIPAYLRKPRGVSRPPVVLFISGLDSAKEEHATFER
                     FFLGRGLATLALDGPGQGETWYKMKMRVDFEVTATAAIDWLSRRGDVDGSRVGICGVS
                     LGGYLAPRCAAFEPRLRAVASCGGLHTLEHRSFVSEHHLRRFCHIWGAKDEADVRVKS
                     KGATLDGVAKNIRAPLLIVHGEQDNLIPPSDPYRTYEQASGPKQWVCYPEGNHVCNNI
                     IYKYRPLVADFMKEHLG"
     gene            13296..14237
                     /locus_tag="VLF19_04435"
     CDS             13296..14237
                     /locus_tag="VLF19_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE92533.1"
                     /translation="MTGHTVPRAWKTGMVEVAPKVFAYVQATGETGISNSGLLVGREA
                     AIAVDALMVPSMTRRFVAAIKRTTRKAVARLINTHHHLDHTGGNRFFHGATIIASEKC
                     REELAPGFPPVPLLQRFMPAFAAEFPKLKVELPTVTFNDRLVLYDGDREIHLWHPGVP
                     AHTAGDATVFLPRERVLFAGDLAFHYVTPLAFQGHVGHWIEAADRVLEAAADVVVPGH
                     GPIGTGEDVRRMRDYLVLVRREARTRFDAGMPAEAAARDIKLGVYASWREAERILPNV
                     MRCYQEFRDETHLPMDIPKMLEGMARLRGVRGDHACL"
     gene            14260..14775
                     /locus_tag="VLF19_04440"
     CDS             14260..14775
                     /locus_tag="VLF19_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018016330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92534.1"
                     /translation="MTATVHELLARNVARFVDRQPDWDAFADARVDGYRRAQHRYIGA
                     GASGKVDPRVIPAEHFTLSVMFVPPGQGNAAHSHEVEEVFFVLDGKVKVFMEDAAGNR
                     AEAVLGRWDCVSCPADMVHGFENVGLEPAYLQVMLGKARPGLMSYADSALQRHRDAHL
                     TGAPAPSVDPR"
     gene            complement(14842..14994)
                     /locus_tag="VLF19_04445"
     CDS             complement(14842..14994)
                     /locus_tag="VLF19_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HSE92535.1"
                     /translation="MSLLLVVILILLLVGLLPTWPYSSGWGYYPSGGLGLVVLILIIL
                     LLTGRL"
     gene            complement(15085..16350)
                     /locus_tag="VLF19_04450"
     CDS             complement(15085..16350)
                     /locus_tag="VLF19_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92536.1"
                     /translation="MSDSVLVIDNDPNIVELLSYFFERRGYTVHTAMDGTEGLRQAIA
                     HRPDVVICDVIMEPVHGFQVLEDIRARRELDDTVVIMVSAKSFRPDIERALQLGADDY
                     VVKPFLMDELLLTVQQRCVARHKGTMTVRFWGTRGSIATPGPATTRYGGNTSCVELRC
                     GGEVLVFDAGTGIRALGESLLREFKGRPLTVHLFVSHTHWDHIQGFPFFTPAYQVTST
                     INVYGPSGQGRPLEKLLRGQMDPDYFPVALGDMGAAIRVHEYRTGEFAIGDTTVRAHY
                     LNHPGMTLGYRVTHGGRSVVYATDNEPYRHTLPRFSRRAETAEGFGALLDENLVRFVA
                     DADLYIGEAQYTDEEYPRKVGWGHSSVSATVEVALQARVKTLALYHHDPMHTDDVVTA
                     MEATARRIIESRGAAMRCFAAQEGQVIEV"
     gene            16456..17211
                     /locus_tag="VLF19_04455"
     CDS             16456..17211
                     /locus_tag="VLF19_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92537.1"
                     /translation="MGRRTVLVTGLSGLIGGAVLEHVAGAYDLRGLSRRAVPGVPCHQ
                     ADIADLGAIEPAFAGVDTVVHLAARAGASPPWEAVLQANVIGSYNVFEAARRAGVRRV
                     VYASSGATVSGWERESPYRELASGRYDDAGAWTTMTHETPVRPVGLYGASKVWGEALA
                     RQYSDAHGLSAICLRIGHVVTEDRPLTVRDYSVWCSQRDIARAIARAIEAPDAVRFEI
                     VFITSRNRWSYRDLAHAKAAIGFEPLDAAEDYR"
     gene            complement(17316..17522)
                     /locus_tag="VLF19_04460"
     CDS             complement(17316..17522)
                     /locus_tag="VLF19_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92538.1"
                     /translation="MWNTALWIDVATWAAVLALPVWLLIEEIVHRAPRFRAVTPRARA
                     PRPAVVRPAPASQGRVDVESTAHA"
     gene            complement(17781..18941)
                     /locus_tag="VLF19_04465"
     CDS             complement(17781..18941)
                     /locus_tag="VLF19_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE92539.1"
                     /translation="MNAPAVQPFTIAVPDAVLGDLRARLERVRWPDEAPGALWRYGTD
                     LAYMKALCAYWRDAYDWRAQEAALNRLRQFTVELGGVPLHFVHEPGVGPRPMPLLLSH
                     GWPGSIVEFQRLIPLLTDPARFGGNPADAFTVVAPSLPGYAFSFRPDQPRLGIGAIAD
                     VFAALMSDVLGYRRFAAQGGDWGAFVTSRLGYAHADRVVGIHVTLLAVRRDTIPAAGA
                     SDEELEFHEQLEHWLREETGYQWIQGTKPQTLAYALTDSPVGLAAWIVEKFRTWSDCG
                     GDPERRFTKDVLLTNVMLYWITGAIGSSFWPYYDRFHTPWPIPDGAQIRVPAAYAAFP
                     REIIRPPRSLAERVYGDLRRWTRMPSGGHFAALEEPEALAADIRAFFRELRQ"
     gene            18993..19916
                     /gene="nikB"
                     /locus_tag="VLF19_04470"
     CDS             18993..19916
                     /gene="nikB"
                     /locus_tag="VLF19_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013385604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HSE92540.1"
                     /translation="MLQYTLRKLLHTALVALGVVTLVFVALRASGDPAATMLPGDASV
                     EELTALRHELGLDRPLHVQYAHFLVGVASGDFGTSFRHQQPALPLVLERLPATLELAF
                     AALVLAVAVALPLGIVAALHRGRLPDVLAMGFAVVGQATPYFWMGIMLILVVSVELGW
                     LPTSGRGRWEQLVLPAITLGTHFAASLARLTRTSMLEVLGQNYVTTARAKGLGERRVI
                     LAHALKNAAVPVVTLIGLQFGTLLGGAVVTETIFAWPGVGRLAVQSIFVRDYPVVQAG
                     VLVLALTFVAINMLVDLLYGYLDPRIRTETA"
     gene            19951..20847
                     /locus_tag="VLF19_04475"
     CDS             19951..20847
                     /locus_tag="VLF19_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92541.1"
                     /translation="MITRPVESVLTADTSRWPHRIPWPLVVPAIPVLIAVLAALLAPW
                     VAPHSPTSGQLAARLKPPAWVDGAAAGRLLGTDLLGRDLLSRLVWGARISLLIALSAT
                     VLGAAAGSLVGLVTGYYRGRVDRVVTKLIDVQLAFPFVLLAIAVIAVAGPSVAVLVVV
                     LAIGAWVGHARIVRGLVLSLREREYVQAAHALGAATPRILFRHLLPEILSVIVVLATF
                     DVGRIIILESTLSFLGLGVQPPTPSWGSDLRDAAAYVRQAWWTAAFPGLAIMVVVLGL
                     NLVGDALRDALDPRTVAPRGRA"
     gene            20876..22405
                     /locus_tag="VLF19_04480"
     CDS             20876..22405
                     /locus_tag="VLF19_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92542.1"
                     /translation="MVTTWTRWALCLVLAAALAAAAPPAGAQRGTQITISQPSDATTM
                     DPARSTQVLTVNYFVNLYDTLTRWDTSLKLQPGLATAWKSVNDTTWDITLRPGVKFHD
                     GSPLTAADVKAVYERARTPGKSVVAAGFATIAAIEVTGPTSLRIVTKKPDPLMPVRMA
                     QMGGYVYPARFANDEGAKELARKPVGSGAYRFVSWTKDDQLVMEANRDWWGWSDKPPT
                     IDRVVWKPIPDDFARLSALQRAEVDVITNVPPDQMKIVRTLTVPATRTVTFWINTTQP
                     PASDARVRQALHYAMDVQSIVKNLYAGQGKPFSGGLADTDFGYNPDLKPYPYDPERAK
                     KLLAEAGHAGGIDVTLYAGSGTMVNDKQLLEAIADMWSKVGIRARIEMMEMAARQKMN
                     NERTVPPNGLMLINPQSTLLDADGSLWRLFHPTGFGGKYWAGNQPGQRFHDLMEQGRY
                     TLDPKKRKALYTEATRIIHDEKPWLELFQEVVIYGVSKRTSFKPRADYRLIVAEMTVG
                     K"
     gene            22407..23324
                     /locus_tag="VLF19_04485"
     CDS             22407..23324
                     /locus_tag="VLF19_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92543.1"
                     /translation="MRRLLLAVVLAPPALGAAAAQRDEAPSMYHIHGLAFAGADPEVL
                     LVATHTGLVRLRSGAPLEWVGEHRFDLMGFAAHPTQAALVFASGHPDVQTYARERVGN
                     LGLLVSGDGGRTWRSAVLRDEADFHALAYSPADGGRLYGWSVVGHAGLYRISTSDWSV
                     QRLPAPGLEDVLGLAASPDAAGTLFAGTTQGLMASRDGGATWRAVAGLPRLPVTAVAY
                     DVADAKVVYAALLRGQGTVMRSPDGGVTWRRTSFETERETAVVALAAGPGGHVAAATT
                     GADVVRSRDGGRTWTKILERGRVSGPAPD"
     gene            complement(23353..23475)
                     /locus_tag="VLF19_04490"
     CDS             complement(23353..23475)
                     /locus_tag="VLF19_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92544.1"
                     /translation="MAPESGLNEMPRFYGQVIASLVQRAAVLFDVAVLQRGEAA"
     gene            complement(23494..23790)
                     /locus_tag="VLF19_04495"
     CDS             complement(23494..23790)
                     /locus_tag="VLF19_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92545.1"
                     /translation="MLARLILAGEFLCGGESDVSFRQRGVPFAAPAVSDEDVTWGLYA
                     LAHHDIYDWLGLTFRFALGPDGRVRGATVVELTEETYTWVKPLLDCDFTRQFVG"
     gene            23858..>24223
                     /locus_tag="VLF19_04500"
     CDS             23858..>24223
                     /locus_tag="VLF19_04500"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSE92546.1"
                     /translation="MPRALYARLAVVLLAAAWLAAAPAAGATSLEEVIRGLESAYSRL
                     TDLRAEFTQTSFNKSLNSTIPARGAVYLKKGGKLRWEFAEPAKQEIVSDGATLWVYTP
                     SLNQVNTGPAPEALAGPAGS"
BASE COUNT         3384 a         8673 c         8598 g         3568 t
ORIGIN      
        1 gggtccgccg acccctgatg cgcagcgcgt cgtcgctctg gtgctatttc catcttcggg
       61 tggcgcggga ttccgtgggc ggggaacagg tcggcggacc cgcgcccccg accccacccc
      121 ggccgacagt cggcgaactt acgcagactg gtgacgcggg ccggacacct ctttgactcg
      181 ccatgctcct ggggcgcggg aacggggcgc gccgtcggtg aacattcgca gcgacttcaa
      241 aacgcgtgga gagtcgggta cgatcatacg tcgtgcgaga attcgaccgg gaccccgtcg
      301 cacggacgag cttgctctgg ttgtttcctc tcgtcggggg actgctgccc ttcgcggcca
      361 ccatcgcggc cttcgccctc tacaacacgt tgttcaccgg ccacgtctgc aaccccttcg
      421 tcgacggctg cgtgtccatc agccgcaccg cgcgctacgg ggtggcgaac cacctcttcc
      481 gcatcctgat gctgccggcg gccgccctgc aggcgctgac gtgggtgctg tgcagctact
      541 ggctgagcga catcggcgcc gtgcgagacc gcgcgctccg catcctgccc tggctgggcg
      601 tggcctcggc gctccttctg ggcgtctacg cgaccctgct cggcaccccg gggcagccct
      661 acagcctggc gcgccgctac ggcataccga cctacttcgt cggcacgtac ctctgcatgg
      721 tgatcaccgc cacgataatc agccgcttcg agggcccgac ctcgacgacg gtgcccgcgc
      781 gcttccagcg gctgctgttc ctgctcgcgc tcttcatgac cggcgccggc ctcgtgcaga
      841 tcggcgtgcg cccgttcatc gtcgagaaga gcctgcgggg ccgcctgacg aacgtcctgg
      901 agtggaacac cgggctggcg ttcacggtgt tcttcaccgt gctggcgtat ctctggtatc
      961 acacgcgctt ctcggtccag ccggcgacgg ccggccggcg gaccctcgcc gccggctcgg
     1021 cgggcggtga gccggcgggc gagctcctcc aggtccggcg gcgcactcag tagcggacgc
     1081 gcacgcggta ggtgagcctg gccgcgccct ccgcggccac cggcaccgcg aagcggaggg
     1141 tgtgcgcctc gatcttctcg gcggcgtgcg tggcgtgcag cacctgccag tcccccggca
     1201 ccggctcgat cacggtgacg gtctgcgcct ccttcttgtg gttgcgcagt gagatctccc
     1261 actcgacctc ccacacgtgc gaggcgatct tgcggaagtc ccgctgcgtc cgctcgccga
     1321 ccacgtcgaa ggcgtcgccg accttgatct ggacgcgctc gtccttgggc gtgtgctcga
     1381 tccagtcctc cccgacgaac tgctggctgc cggcggtgtc ggccttgtag acgcggagct
     1441 tgcccttcgg gagcggcacg ccgaggcggt tgtccttgct gttgcgcagc tccaggtaga
     1501 cgccgatctt ctgatgcgat accgggactc cgtactggtt gcggtagtag tcctgggccc
     1561 catagtagat cagatgcttc ttcacgccga cgtccgaggc ggcgaggagc gagagttgct
     1621 tggtctggtt gtccttgatc gtggtacgcc cgtcgagcgt gtacaggtgg tactcgaaga
     1681 acccctcgga cgcgaagtcg cggcgggcgt cggcggccgt cgccgccttg gccgccgtct
     1741 ccagcacccg cccctcgcgg cggggatcac gcgcccggtt gacgtcgccg gccacgagct
     1801 taagcgtggc gctccggtac gtcgccccgc tcttgttgtc gatcgtgacc cagccggtca
     1861 ggtcaccgcg cgtgtcggcg gcgttcacga ccagcacgta gtcggccttc cacgtgatgc
     1921 cccccgtgag gtaggacgcc tccacgcgct gctgggtggc cgtctggttc cggagcagcc
     1981 acgcgagggt gggccgcagg accaggttgt ccggcagcgc cggcaggatg agacgccccg
     2041 tgtggccgag gtggatctgg ccgttgatct cgtagacggg gccgttcgtg gacagcagcg
     2101 tcgcgtcgtg gaaggtgccg tcggactggt agagccgcac cttcttgccg acgtacttct
     2161 ccatgagctt ctggctggag agcaggtcgt actcgtaatt ctgctcgagg atcttgagcc
     2221 cggccgggtc ggtgagcgac ttgaggtgca ccgtcgtggg gtcgatcagg gcggccacgt
     2281 ccatgaactg cacctcggtc gtccccggcg gcagccgcgc ctcgcgcacg tccttcacga
     2341 ggccgaggtt gccgttgtag accgtcacca tgatgttccg ctgggtctcc gccgtgaccg
     2401 tcagcggcgc ggcgggcacc tccgccggca cggcgagcac gccgatcagc aatgccgcga
     2461 tgagctccct cttcatcgtc gtcctccggg cgatcggatc gactgccttc gtccggttgg
     2521 acagggccag cgacgaaaag ttcccggcgc ggcccggtcg agctcggtcg cgatgacgcc
     2581 tgtcggccca ggcgccggcg cgcccatgag tcgagaacgg aagcgctgat tcagcgggtg
     2641 aggcggatga cgacgtggac ggacgggggg agcgccggcg gctcggcccg cggccgccac
     2701 tgaccgatgc tcgtcagctc gcgaacaaga tcggggtatc gctgccgcgg caccgcaacc
     2761 tcgaggagca gcgcctcgcc gtcctcgcgg cgggcggcga cgctgccgcc cagcccgcgg
     2821 gcgatgtcct cgatcttcgc ggcggccgct cgagcgtcgg cgacgagcag agtgccaccg
     2881 gcgtcggcga cggccgcgct tcctgcagcg ccaccgagcc gtcccgcgat ctcgtcacgc
     2941 gcgacgtcct cgtgaggcgt gctgtcgcgg gccgtgccgt cggcctgcgt gccgggcggc
     3001 gccgcctggc ctgtggactc ctgcgcccgg gccgatttct gtcgccggtc ggacccgagg
     3061 ggcagcgggg cctcgcgcct gggcggtgct tccggccggg gtgcgctcac gccgtccgcg
     3121 gcctcgcccg atgctcctgc ttcctggcgc gctcccggcg cttgcgggga cgctgacgct
     3181 ttcggcgctt ccggcgctcg cgacggggcc ggcacgtgcg gcgccggggc ctgcgtcggt
     3241 acccgcctgg agacctgtgg gtccttggcg aggtcgccag cggcgtcccg cgcgccccag
     3301 ctctgatcct gacgaatggc cggcggctct gcaggtcggc ttgcgacgcc gggcgccgga
     3361 cccggaagct cggactctcg accgccgacg ccgggagccg gcgccggctg ttcgatgcgc
     3421 gcgcgcggct catcgacccg cgccgcgcgc tcctgctcct cggtaccccg gaacaccagc
     3481 accgcgaacc ccccgacgag cagcagcgct gcggcctcga gcggcagctt caccgtccac
     3541 ggcaggaaga gccggcgcgc gagccgcgtg gtccagggct cggggcgcgc ggccgccacc
     3601 agccggtcca cgaagcctgc cggcgcgcgg tccggcggaa gcccccggac cagggccacc
     3661 gcgccccgga agccctccag ctcgcggccg cactcgccgc aggtcgccag gtggccgtcg
     3721 agccgcgcgc ggtcctccgc cgtgagcgcg tcgtccagca gcgccgagaa cagctcgcgg
     3781 gcgtcgtgac aggtcacggc aggaaacgct cgagcttggc cttgaggtcc gtgcgcgccc
     3841 ggtgcagtcg cgagcgcacc gtgcccggct cgaggtcgag cgccgcggcg atctcgtcgt
     3901 acgagaggcc ttcgacgtcg cgcagcacga ccacgatgcg ccgttcctcc ggcagctccg
     3961 cgatcgcctg gcgcagtgcc gtctccagct cgccccgctc gagcgccgcc gccccgtccg
     4021 cctcgccact cggcgcctcg cgcagggcct cctcatccgc ccgctcgcga cgccgggcgc
     4081 ccgaggccag gcggttcagg cagagccgcg acgtgatcgc gtagagccag gtggagagcc
     4141 gcgcctggcc ccgaaagtcg ccgaccgacc gatgcacgcg caggaacacc tcctgggcga
     4201 tctcctcggc ctccgcggcg ctgcccagca tgcgcgcggc cacgccgaag acccggtgct
     4261 ggtatgtcct caccaggtcc tcgaaggcgg cgggttcgcc ggcgcgcagg cggtcgacga
     4321 ggccgggcgg atcggagacc ggccctcgca ccgttagacc ggccccgccg tcagaagttc
     4381 cggcgccggc cgcatgcgga accgatactc gtgcgcagcg ccgtccgtcc ggtcgaggac
     4441 ctcgtcaccg tccgccgcca tcacgtcgtt cggcggccgc cgcgcggacg cgaagaccgg
     4501 ttccggcgcc gggcacccgg ccagcggcgc gcgtggaggg cggacagcag gagcttccgg
     4561 aagctgccgt cgcggtcgag gagcgcctga ccctcgcgtg gcagcagggc cgtcgccatc
     4621 cctcggagct tagcgcaggt gcgcgccgcg acgcttctgg tatgttatcc gggcgacacc
     4681 ggccatgaag accagcccgt cgatgcggct gcggcgatga cgggcgcgct gccactcgag
     4741 ggagtgcgcg tcctggagct cgggcacatc gtcgccgggc cttcggcggg actcctgctc
     4801 gccgacctcg gcgccgacgt cgtcaaggtg gagcggccgg gagcgggcga ccagacgcgc
     4861 gggatgccgg cgggcaacgc ggcgaacttc catttcctca atcgcaacaa gcgcagcgtc
     4921 gccctcgacc tgaagggctc tgccgagggc cgcgagctct tcctgcgcct cgtgaagacg
     4981 tccgacgtcg tcatcgacaa cttcgcctac ggggcggtgg aggccctcgg cctgggctac
     5041 gacgtcctcg cgcgcgccaa tcccggcatc gtctacctcg cgctcagggg cttcctcccg
     5101 ggtccctacg aggcgcggcc gttcctggac gagctggcgc agatgtccgc gggcctcgcc
     5161 ttcatgacgg ggccgcgcgg ccagccgatg cgcgcgggcg catcgatcgt ggacgtcggc
     5221 gccgccgcct acggcgtcat cgccgtcctc gccgcgctgc agcagcgtgc gcgcaccggc
     5281 gtcggacaga agatcacctc gggcctctac gagacgacgg tcttctgggt cgggcagtgg
     5341 ctcgcgaact tcggcgccac cggcgagccg tcggtgccga tgccggagat ccgtcagggc
     5401 acgcgcatgg gctggggcat ctaccagctc ttcaccgctg ccgacggcga gcaggtcttc
     5461 atcgggatca cctcgaatgc ccactgggag cgcttctgca gggaattcgc gctcgacgac
     5521 ctcctggccg acgagcggct cgacgacaac gccaagcggg tggcggcgcg cgggtggctg
     5581 cccgggcgca tcgccgagga gatgcggcgc tacccgagcg ccgagctggc cgaacggctg
     5641 gagcgcgcgc gggtgccgtt cgcgccgctg cgccgccccg accagctcgt ggacgacccg
     5701 cacctcaacg tctccgggca gttcgtcgac accccgctgc ccggccgcgg cctggccaag
     5761 ctgcccaaga tgccggtccg cagcacggcg ttcgagttcg acctgcggcg cccggcgccg
     5821 cggctgggcg aacacactcg cgaggtgctc gaggaggtcg ggctctcgaa ggacgagatc
     5881 gacgcgctgg cgtcacggag ggtgatcgga tgagcgatat tcccaggtcc gtcaagctca
     5941 aggaagtggg cccgcgcgag ggcttccagt tcgagaaggg cccgatcagc ctggccgaca
     6001 agatctcgct ggtggacgcg ctctccgaca cgggcgtcaa gaccatcgag ttcacctcgt
     6061 tcgtgagccc gaggtgggtg ccgcagatgg ccgacgccga gcagatggtg gccggcatca
     6121 agcgtgcccc cggcgtcgcc tacgaggcga tctggctcaa cgaggcgggc ctcgagcggg
     6181 ccgcccggct gcgggagtcg ctcgcgctgc gtccgatgtt cagcgtggcg ggtagcgaca
     6241 ccttcatgcg caagaacacc aaccgcagcg tggacgagcg gctcgacgag ctgccgggct
     6301 gggcggagcg gtatcgggcg ctgggcatgg actggctcga cgtgggcgtg atggcggcgt
     6361 tcggctgcaa ctacgagggc gacgtcgagc cccgtcacgt ggtgacggtg ctgcagagag
     6421 tcgagcagag ggcggacgcg tgcggctcgc ggctgggcgg catcggtctc gccgacacga
     6481 tggggtgggc caatccgctg cagatcaggc gtctggtggg cgcggtgcgc gagcggtggc
     6541 ccgacgcgcc ggtgaggctg cacctgcacg acacgcgcgc gctggccatc gccaacgtgg
     6601 tcgccgcgct cgagatgggc atcgactgct tcgacagcgc ggtggccggc atgggcggtt
     6661 gccccttcgc cgcccacaag ggcgccgcgg gcaacgtcac cacggaggac gtggtgttcc
     6721 tgtgtcagga gatgggcatc gacacgggca tcgacctcga cgcgatgatc gcggcggggc
     6781 agctggccga gcgcgtcgtt ggacacccgc tgcccggcaa gctgaagtcg ggcgggaatc
     6841 tcgcgacgta tcgagcccgc gcgcgcgccg ccggggcgcc ggcggcggtc tgagcgaccg
     6901 acgggtcggg tcatggacgc tcaggcggag gccgggacgt cgcgccgcga cttcctcgcc
     6961 accactccgc gcggcatcgc cgccggaact ctggcgggcg cggcgatgac ggggacctgc
     7021 tcgctctgaa gtccggtgag cgcgtcctcg acgtcggcgg cggcccccgt gcggtcacgc
     7081 gcgattcggc ccgacgggtg acggcgccgt cgtcctagct cggcgatgat cgccggcgct
     7141 cgctcgcggc ccggcgacga gccatgctag gattccgggc catgaccagc cacgacaccc
     7201 ggaacatgcg gttcctctcc cagcaccccc tcgacggctt cggcaacgtc ggcgagggca
     7261 tggccgtgca gctcacgcgc gatcggcggc ggatcctctg gctcgcccac gagtcggcgc
     7321 cgaagaacgt gacggccgtg gacgtcacga accccaagaa gccggcggtc gtcctccaga
     7381 ccgacctgcc gcacgagcgg atgcgctcga acaacctcga cgtggtcggc gacctgctcg
     7441 tgatcgccta ccagacgagc gcccccgggc tgacgcccgc gggcttcgag atcctcgacg
     7501 tcgccgatcc cgccaagccg aagaaggtcg gcttcttcga tgcctccggc ccgacgtcgc
     7561 gcggtgtgca ccacctgtgg ttcgtggacg gctcgtacgt gcactgtgct tcgggggccg
     7621 cggacttcaa gccacgcaac ccgcgggacg atcagttcta ccggatcgtc gacatccgcc
     7681 agccgtcgaa gcccgtcgag gtgggccggt ggtggctgcc cggcacgcgc gacggcgaca
     7741 ccgagccgcc gccgccgcgt cacccgaagt tcgatgcggg ctaccgcgcc cacaacacga
     7801 acgtctatcc gcagcggccc gaccgcgcct acgtcggcta cctcgacggc ggggccatcg
     7861 tgctcgacat ctcggacatg ggcaagccga ggatggtcgc ccgctgggac taccagccgc
     7921 cgatgccggg cttctgccac acggtactgc cgctcttcga gcccggcctg ctcgtggtca
     7981 gcgacgagtc caccaaggac ggcgccgccg actggcccaa gctggtgtgg gtcctcgaca
     8041 atcacgtgga gaccaagccg gtgccgatcg cgacgtgtcc gctgccgccg ctcaagaagt
     8101 tcgccaagcg cggtggccgc ttcggcgccc acaacctgca cgagaaccgg ccggggccgg
     8161 gcacctggca ctccgagaag gtcgtcgtgg ggacgttctt caatggcggc gtccgggcct
     8221 tcgacctctc cgaccccttc cgtccggcgg aagtggccta cgcggtgccg ccggcgccgc
     8281 gcaagtcgcc caagaaggcg gtccagctca acgacgtctt cgtggacgat cgcggcgtgc
     8341 tcttcaccgt cgatcgcatg atcggcgggc tgtacgccta cgagctcaag gtctaggaac
     8401 gaacgcgccg gacctcggag tggagggacg acgatgaagc ggctgtcgcg cagggacttc
     8461 ctcaggacga cggcggcgtc gacgctcgcc ggcgtgggcg cgtgggggcc ggtgcacgcc
     8521 cagccgtcca agaagacgct ggtctacgcg aagaacgagg acatcggcca gctcaacccc
     8581 tacttcgtca atcacgggat cttcgaggcg ttgaacaacc acatccagga gccgctcgtc
     8641 cgcttcgact acgagaagcg ggcctgggag cccgtgctcg ccgagtcctg gacgacggaa
     8701 gagcggggcc ggacgtgggt gttccgcctg cgcgaggcga agttccactc cggcgagccg
     8761 ttcacggccg acgacgtcaa gtacacgttc gagcacgtcc tcaaggagaa gtcggcggga
     8821 ccgatcaagg ccaacctgtt catgatcgac acgatcaccg tgcgcgaccc gcgcacggtg
     8881 gccatccagt gcaaggggcc gacgccgggg atgctgtcct acatccagtc gcgcccgtcg
     8941 gtgatgagcc gcgcgctcgg cgagaaggtc ggcttcgacg aggcccacaa gaagatggtc
     9001 ggcaccgggt cctaccgctt cgtcgagtac gcgcgcgacg agcacctgat catggagcgc
     9061 aatcccgact attggggcga gaaggcgagg atcgagcgca tcgtctaccg gcccatcccc
     9121 gacaacaccg cgcgcgtgac cgcgctccgg gccgggcagg tggacgccat ggcccaggtc
     9181 gcgccattcg acatcggcaa gctcgagcag gtgccggacg tcaagctcta ctccgtgcgc
     9241 gcggcgcgca tccacatgct cttcatgaat cccatcacgc cgccgctgca gaacaagctc
     9301 gtgcgcctgg cgatccacca cggcgtggac gtggacacca tcgtcaagac ggtgctcgag
     9361 ggccgtgcct tccggctcag ccagctctgc gggccgtcgc aggtgatgac ctacgatccc
     9421 gacctcaagc ccctgcccta cgatcccgag cgcgcgagga agctcctggc ccaggccggc
     9481 taccccaatg gcgtggacct cgacttccac gactatccgg cctacgcgga gtacaagccg
     9541 atgggcctcg ccgtcgccga gcagctcaag aagatcggcg tgcgcgtgag tatcaagccg
     9601 ctcgagacga gcgtgttccg gcgcaagtgg ttcgcgggcg acctgtcctt ctacttcatc
     9661 ccctacggca acgtggccga ggacgcctcg gcgttcctga aggcctactt ccgctccgac
     9721 caggacaagc gctcgcgcta caagaacccg gaggccgacg cgctgttcga ccagcaggag
     9781 gccgagctcg atcccaagag gcgccaggcg ctcaaccggc ggctcatgcg gctcttgcag
     9841 gaggacagcc cggcggtgcc gttctacaac cctgagtacg tcgtcgccgc gcgcaagcac
     9901 gtgcagatcc cagcggggat ccccaccggc ggcgagtacg tgtggttctg gaagatggac
     9961 gtcgcctgac gggaatcccc gatggggcgt tacctcgcca cacgcctcct ggcgagcctc
    10021 ctgaacctcg tcctcgtcac cgtgctcgtg ttcgtcatgc tgcacgtggc gccgggcgat
    10081 ccggcggact acctcgtcgg tcccgacgcc accgaggccg atcgggccga ggcgcgccgg
    10141 ctgtggggcc tcgatcgtcc gctgccggtg caatacctcg cctacgcggg caacgccctg
    10201 cggggtgatc tcgggacctc gctctacttc caggaacccg tggcgcagct catccgcaag
    10261 cgggcggcgg ccaccctcga gctgggtgcc ctggccgtgg cgatcgtcct gggcgtctcg
    10321 ttcgtgctcg gcgtcaccgc gggagcccgg cctcacggac tcctcgatca gaccgtgatg
    10381 atgctggccg tctgcggcgt cgccatgccg gccttctggc tcgccctgct gctgatcctg
    10441 gtgctcggcg gcgcgctgca gctcctgccc gtcgccgggc gactctcgta tggcacggga
    10501 ctcgcttcga tcaccggctt ccacctggtc gacagcgtgc tcaccgccaa cggcgcggcg
    10561 ttctgggact cgctgcgcca cctcgtcctg ccggccgcgg cgctcgcggg ccccggcatc
    10621 gcgctccaga gccggctgct gcgctcgaac ctcctcgagg tcatgggcga ggactacgtg
    10681 cgcaccgcgc gcgccaaggg cctcggcgag cccgcggtga tcggccgtca cgccgtctgg
    10741 aacgcgctga cgccggtgct ctcctcgtgg ggcgtgctct tcgtcttcct gctctcgggc
    10801 tcggtgatcg tggaaacgat cttcgcctgg ccggggctcg gccgcacggc gatccaggcc
    10861 atcaacaacc gcgactaccc gctggtgcag ggcgtcgtgc tcgtcttcac ctcgatcgtc
    10921 gtggtcgcca acttcgcggt cgacgcgctc tgcgcgtgga tcgacccgcg cgtgcactat
    10981 gggcgggggc cctcgtgagc gagcgctccg ccgtcgccga gcgggccggt gtggatgcgc
    11041 gccgcgacgt cgccgcgccg accaccgtcg ccgcgcgcgc cgggccgtgg cacacgcgcc
    11101 ccggccggct gcgctgcgcc gttggcggcg ccgtggtcgt cctgctgctc gccgtggccg
    11161 cgctggcgcc cctgctcgcg ccggcgtcgc cggacagcca ggacctcctg cgccggctga
    11221 cgccgccggc gtggatgccg ggcggcagcg ccgccaacgt gctcggcacc gaccacctgg
    11281 ggcgcgacat cctgagccgc atcgtccacg gcgcgcggat ctcggcgatc gtcggcgtgg
    11341 tggccgtgct cgtctccggc gttctcggga ccacgctcgg gctcgtggcc ggctattacg
    11401 gggcgcgcgt ggatctcgtg gtgatgaagc tcgtggagct gttcctcacg atccccttcg
    11461 tgctgctggc gctggtcgtg atggcgctct tcggccaggg gctcgccatc ctgatcgtcg
    11521 tcctgtgcct caaccgctgg atccactacg cgcgggtcgt gcgcggcgac gtgctctcgc
    11581 tgcgcgagcg cgagttcgtg gccggcgcgc gcgcactcgg cgcctccaat gcgcggatca
    11641 tcgcgcgcca cgtgctgccc aacaccgtcg cctcgctgag cgtggtgacc accttctcgc
    11701 tggcgacgat catcctgatc gaagcgtcgc tgagcttcct ggggctgggc gtgccgccca
    11761 cgacgcccac ctgggggggc atgctcaacg agtcccgctc ctacatgatg ccgtcgtggt
    11821 ggacgtccgt cttccccggc ctggcgatct tcgtcaccgt gctcggcgtc aacctgctcg
    11881 gcgacggcgt ccgcgacgcg ttcgatccga ggctgaagaa gaaggcgtag ggcgacccgg
    11941 agcgtccccg acaaggagag agtcccatgc ccgatgcgat cctcacgcag gtgatcaaga
    12001 gctggaagcc gcgcttcgtg aacaacggca tcgacatgaa cgactatcag tggacggtcg
    12061 agccactgga gaagtgggag cagtggtacg acgcgtggat ggcgctcggc cggaagcacg
    12121 aggacatggc gttcgaggcc gagaaggccg gccgtgccgt caccgccggc gaggcctact
    12181 accgggcggc gctcgcctat cacttcggcg tcttctgctg ggtcgacgac ctcgaccagt
    12241 acggcgtcgg cgccgccaag cgcgtgtcct gttacggcaa ggcgctggcg acgctggacc
    12301 cgcccggcga gcgcgtggag tttccgctgg agcggatcac gatccccgcg taccttcgca
    12361 agccccgcgg cgtgtcgcgc ccgccggtcg tgctgttcat ctcgggcctc gactccgcca
    12421 aggaggagca cgccacgttc gagcggttct tcctcggtcg cggcctcgcc acgctggctc
    12481 tcgacggtcc cggccagggc gagacctggt acaagatgaa gatgcgcgtg gacttcgagg
    12541 tcacggcgac agcggccatc gactggctct cgaggcgggg ggacgtcgac ggctcgcgcg
    12601 tggggatctg cggcgtcagc ctcggcggct atctcgcgcc acgctgcgcg gccttcgagc
    12661 cgcggctccg cgccgtcgcc tcgtgcggcg gcctgcacac cctggagcac cggagcttcg
    12721 tgagcgagca tcacctgcgc cggttctgcc acatctgggg cgcgaaggac gaggccgacg
    12781 tccgtgtcaa gtcgaagggc gcgacgctcg acggcgtggc caagaacatc cgcgcgccgc
    12841 tgctgatcgt gcacggcgag caggacaacc tcatcccgcc ctcggacccg tatcggacgt
    12901 acgagcaggc gagcggcccg aagcagtggg tgtgctaccc ggagggcaac cacgtctgca
    12961 acaacatcat ctacaagtac cggccgctgg tcgccgactt catgaaggag cacctcggct
    13021 gagccgggcc tggccgcggt cggccgcgtg gcggcatcgt gccgccctcg tgccagggcc
    13081 ccgatgcgcg gcctcggaca gcagcgggag cgtgccgcgg agcgcgatcg acgtctcgcc
    13141 gatcgtgcgc ggccccgacc ggggtggtgg ctcgaggcgg cctcgccggg gcccaggtgc
    13201 accgcggcgt gacccggcgc aacgcccgtc cgcgccttga cacgccggac gggcgcgtga
    13261 taaccgcgtg gcatgtctcc gtcgggccgc gcgcgatgac cggtcacacc gtgccgcgcg
    13321 cctggaagac ggggatggtc gaggtcgcgc cgaaggtctt cgcctacgtg caggcgacgg
    13381 gcgagacggg catctccaac tccggactgc tcgtcggccg cgaggccgcg atcgcggtgg
    13441 acgcgctcat ggtgccctcg atgacccgcc ggttcgtggc cgccatcaag cggaccacgc
    13501 gcaaggccgt cgcgcgcctg atcaacacgc accaccatct cgaccacacc ggcggcaacc
    13561 gcttcttcca cggcgcgacc atcatcgcct cggagaagtg ccgcgaggag ctggcgccgg
    13621 gcttcccgcc ggtgccgctg ctccagcgct tcatgcccgc gttcgccgcc gagttcccga
    13681 agctgaaggt cgagctgccc acggtgacct tcaacgaccg cctcgtgctg tacgacggcg
    13741 accgcgagat ccacctgtgg catccggggg tgccggccca cacggccggc gacgcgacgg
    13801 tcttcctgcc cagagagcgc gtgctcttcg ccggcgacct ggccttccac tacgtgacgc
    13861 cgctggcctt ccagggccac gtcggccact ggatcgaggc ggccgaccgc gtgctcgagg
    13921 ctgcggccga cgtggtcgtg cccggccacg ggccgatcgg caccggggaa gacgtcaggc
    13981 gcatgcgcga ctatctggtg ctcgtgcgcc gcgaggcgag gacgcgcttc gacgcgggca
    14041 tgccggccga ggcggcggcg cgcgacatca agctcggggt ctacgcgtcg tggcgcgagg
    14101 ccgagcgcat cctgcccaac gtgatgcgct gctatcagga gttccgggac gagacgcacc
    14161 tgccgatgga catcccgaag atgctggagg gcatggcgcg ccttcgcggc gtccgcggcg
    14221 accacgcgtg tctctgaggc cgggccggga ggactcgaca tgaccgcgac cgtgcacgag
    14281 ctgctcgcgc gcaacgtcgc ccgcttcgtg gaccggcagc cggactggga cgcgttcgcc
    14341 gacgcgcggg tcgatggcta ccgccgcgcc cagcaccgct acatcggggc tggcgcctcc
    14401 ggcaaggtcg atccgcgcgt gatcccggcc gagcacttca cgctgagcgt gatgttcgtg
    14461 ccgcccggcc agggcaacgc cgcccactcc cacgaggtcg aggaggtctt cttcgtcctg
    14521 gacggcaagg tgaaggtctt catggaggac gccgccggca accgcgccga ggcggtcctg
    14581 ggccgctggg actgcgtgtc gtgcccggcc gacatggtgc atggcttcga gaacgtcggg
    14641 ctcgagccgg cgtaccttca ggtgatgctc ggcaaggccc gaccgggcct gatgagctac
    14701 gcggacagcg cgctgcagcg gcaccgcgac gcgcacctga ccggtgcgcc ggcgccttcc
    14761 gtcgatccgc gctgagcggc cgatcggcgc cgaggcgacc gcccgtcgtc gggggcaccg
    14821 cggcggcctt ccccgctcag atcagagccg gccggtgagc agcaggatga tcaggatcag
    14881 gaccacgaga cccagcccac cgctcggata gtagccccac ccggaactat agggccatgt
    14941 tggcagcagg ccgacgagca gcaggatcag gatgacgacc aggagcaagc tcatggcgca
    15001 aaccctccgc cgccacgtag gagcaagcgg agtgccagcg gccacgcgcc ccggagctcg
    15061 gcttccacct cgtcgacacg ctcgctagac ctcgatgacc tggccttcct gcgccgcgaa
    15121 gcagcgcatc gcggcgccac gtgactcgat gatgcggcgc gccgtcgctt ccatcgcggt
    15181 cacgacgtcg tccgtgtgca tcggatcgtg gtggtacagc gccagcgtct tgacccgggc
    15241 ctggagggcc acctcgacgg tggcgctcac cgacgagtgg ccccagccca cctttcgcgg
    15301 gtactcctcg tccgtgtact gcgcctctcc gatgtagagg tcggcgtcgg cgacgaaccg
    15361 cacgaggttc tcgtcgagca gggcgccgaa gccctccgcg gtctccgcgc ggcggctgaa
    15421 gcgcgggagc gtgtgccggt acggctcgtt gtccgtggcg tagacgaccg accggccgcc
    15481 gtgcgtgacc cggtagccga gggtcatgcc cggatggttg aggtagtgcg cgcggacggt
    15541 cgtgtcgccg atcgcgaact cgccagtgcg gtactcgtgc acgcggatgg ccgcccccat
    15601 gtcgccgagg gcgaccggga agtagtcggg atccatctgg ccacgcagga gcttctcgag
    15661 gggccggccc tgccccgatg gcccgtagac gttgatcgtg ctcgtcacct ggtaggcggg
    15721 ggtgaagaac gggaagccct ggatgtggtc ccagtgcgta tggctcacga agaggtggac
    15781 ggtgaggggc cggcccttga actcgcgcag cagcgactcc cccagggcgc ggatgcccgt
    15841 gcccgcatcg aagacgagca cctcgccacc gcagcgcagc tcgacgcacg acgtgttgcc
    15901 gccgtaccgg gtcgtggccg ggcccggcgt ggcgatggag ccgcgcgtgc cccagaaccg
    15961 caccgtcatc gtccccttgt gccgggcgac acagcgctgc tgcaccgtca gcagcagctc
    16021 gtccatgagg aacggcttga cgacgtagtc gtcggcgccg agctgcaggg cgcgctcgat
    16081 gtcgggccgg aacgacttgg cggagaccat gatcaccacg gtgtcgtcca gctcgcgccg
    16141 ggcgcggatg tcctcgagca cctgaaaccc gtgcaccggc tccatgatga cgtcgcagat
    16201 gaccacgtcc ggacggtggg cgatcgcctg ccgcaggcct tccgtgccgt ccatggccgt
    16261 gtggacggtg tagccgcggc gctcgaagaa gtaggacagg agctcgacga tgttcggatc
    16321 gttgtcgatg acgaggacgg agtcgctcat ctcacgtcga gccctatagt acactctcgg
    16381 cgcgcggcac gcgcggccgg cacgacgatg gcggtgacgg ccccgacgtc gggctcaacc
    16441 acgtacggga ggggaatggg tcggcgcacc gtcctggtca cggggctcag cggcctcatc
    16501 ggcggcgccg tgctggagca cgtggccggc gcctacgacc tgcgggggct cagccgtcgt
    16561 gcggtgcccg gggtgccgtg ccaccaggcc gacatcgccg acctcggcgc catcgagccc
    16621 gccttcgccg gcgtggacac cgtcgtgcat ctggcggcca gggccggcgc gtccccaccg
    16681 tgggaggccg tcctccaggc gaacgtgatc ggcagctaca acgtcttcga ggccgcgcgg
    16741 cgggcgggcg tgcgccgggt cgtctacgcg agcagcggcg cgaccgtgag cggctgggag
    16801 cgcgagtcgc cctaccgcga gctcgcctcc ggccgctacg acgacgccgg cgcgtggacc
    16861 accatgactc acgagacgcc cgtgcgaccg gtgggactct acggtgccag caaggtctgg
    16921 ggcgaggcgc tggcgcggca gtactcggac gcccacggcc tctcggcgat ctgcctccgc
    16981 atcggccacg tcgtgaccga ggaccgcccg ctgacggtcc gcgactactc ggtgtggtgc
    17041 agtcagaggg atatcgcgcg ggcgatcgcg cgggcgatcg aggcgccgga cgccgtccgc
    17101 ttcgagatcg tcttcatcac gtcgcgcaac cgctggagct accgcgacct cgcccacgcg
    17161 aaggccgcga tcggcttcga gccactcgac gccgccgaag actaccgctg agccgtcgcc
    17221 gcccggcgcc gacggcggcc gggcggtcgc cgcacgcgcg ccgggcgatc tgaccggcac
    17281 gaaggacgcg tcgggaagcc gcttcggcga gggtctcagg cgtgcgccgt gctctcgacg
    17341 tccacacggc cctggctggc cggcgccggc cgcaccacgg caggccgggg agcacgggcc
    17401 ctgggtgtca ctgccctaaa tctcggggcg cggtggacga tctcctcgat caggagccac
    17461 acgggaagcg ccagcacggc ggcccaggtg gccacgtcga tccagagggc cgtgttccac
    17521 attgccgttc cctccttggc catcgacagt ggcatcagcc gtgccagacc ccgagagcgg
    17581 ggaaatcggc ggtttggacc acgccggccc gtcggggcct tcgggcatgc tgcccgatcg
    17641 ggcacggacg cccgatcgtc ggggggctgc cgggcggcgc ccgcgttagc gtgaagagcg
    17701 tgtccgagta tccacgactc tagcgaagcc ccgcctgacg caacccctcc tggggaaggc
    17761 gacggcgcgc cgcccgcggc tcactgtcgc agctcgcgga agaaggcgcg gatgtcggcg
    17821 gcgagggcct cgggctcctc gagcgccgcg aaatggccgc cggagggcat ccgggtccac
    17881 cggcgcaggt cgccgtagac gcgctcggcg agcgagcgcg gggggcggat gatctcgcga
    17941 gggaaggcgg cgtaggcggc cggcacgcgg atctgcgcgc cgtcggggat gggccacggc
    18001 gtgtggaagc ggtcgtaata cggccagaac gacgatccga tcgcgccggt gatccagtag
    18061 agcatcacgt tcgtgaggag cacgtccttc gtgaaccgcc gctcggggtc gccgccgcag
    18121 tcgctccacg tccggaactt ctcgacgatc cacgccgcca gcccgacggg cgagtcggtg
    18181 agcgcgtagg cgagcgtttg cggcttcgtg ccctggatcc actggtagcc ggtctcctcg
    18241 cgcagccagt gctcgagctg ctcgtggaac tcgagctcct cgtcgctggc gccagccgcc
    18301 ggtatcgtgt ctcgccgcac ggccagcagc gtgacgtgga tgccgaccac gcggtcggcg
    18361 tgcgcgtacc cgagtcgcga ggtgacgaag gcgccccagt ccccgccctg ggccgcgaag
    18421 cggcggtagc cgaggacatc gctcatcagc gcggcgaaga cgtcggcgat cgcgccgatg
    18481 cccaggcggg gctggtccgg gcggaacgag aacgcgtacc cgggcaggga gggcgcgacg
    18541 accgtgaagg cgtccgccgg gttgccgccg aagcgggcgg ggtcggtcag cagcgggatc
    18601 agccgctgga actcgacgat ggatccgggc cagccgtggg agagcagcag cggcatcggg
    18661 cggggcccca cgcccggctc gtggacgaag tgcagcggca cgccgcccag ctcgaccgtg
    18721 aactggcgca agcggttgag cgcggcctct tgcgcgcgcc agtcgtacgc gtcgcgccag
    18781 tacgcgcaga gcgccttcat gtacgcgagg tccgtcccgt accgccaaag ggcgccggga
    18841 gcctcgtcgg gccagcgcac gcgctcgagc cgagcgcgga ggtcgccgag cacggcgtcg
    18901 ggcaccgcga tggtgaaggg ctggacggcg ggcgcgttca tgcgggccca gtatagggcc
    18961 gcgcgcccgc ctgctatact cgccgcgccc ggatgctcca gtacacgctt cgcaagctgc
    19021 tgcacaccgc gctggtcgcg ctcggcgtgg tcacgctcgt gttcgtcgcc ctgcgcgcga
    19081 gcggggaccc ggcggcgacg atgctgcccg gggacgcgtc ggtcgaggag ctgacggcgc
    19141 tccgccacga gctcgggctc gaccggccgc tgcacgtgca atacgcgcac ttcctggtcg
    19201 gcgtcgccag cggcgacttc ggcacctcgt tccgccatca gcagcccgcg ctgccgctcg
    19261 tgctcgagcg cctgccggcc accctcgagc tggccttcgc cgcgctcgtg ctggccgtcg
    19321 ccgtggcgtt gccgctcggc atcgtggccg cgctccaccg ggggcggctc cccgacgtgc
    19381 tggcaatggg ctttgccgtc gtcggccagg cgacgcccta cttctggatg gggatcatgc
    19441 tgatcctcgt ggtgtcggtg gagctcggct ggctgccgac ctcgggccgc gggcgctggg
    19501 agcagctggt gctgccggcc atcacgctcg gtacgcactt cgcggcctcg ctcgcgcggc
    19561 tcacccggac cagcatgctg gaggtgctcg gccagaacta cgtgaccacg gctcgcgcca
    19621 agggcctcgg cgagcgtcgg gtgatcctcg cccacgcgct gaagaacgcc gcggtgccgg
    19681 tggtcacgct gatcgggctg cagttcggca cgctgctcgg cggggccgtc gtgaccgaga
    19741 cgatcttcgc gtggcccggc gtggggcgcc tggccgtgca gtcgatcttc gtccgcgact
    19801 acccggtggt gcaggccggc gtgctcgtgc tcgcgctcac cttcgtcgcc atcaacatgc
    19861 tcgtggacct gctctacggc tacctcgacc cgcgcatccg gacggagacc gcgtgatctc
    19921 gcgtcgggcg cgccgcgcca cgcgatgtcc gtgatcaccc ggccagtgga gagcgtgctc
    19981 accgccgaca cctcgcggtg gccccaccgc attccctggc cgctggtggt gccggcgatc
    20041 ccggtgctga tcgcggtgct cgccgcgctg ctggcaccct gggtcgctcc ccactcgccg
    20101 acgtcaggcc agctcgcggc ccgcctcaag ccgcccgcct gggtggacgg cgccgccgcc
    20161 gggcggctgc tcggcaccga tctgctcggc cgcgacctcc tcagccggct tgtctggggc
    20221 gcccggatct cgctgctcat cgcgctctcg gcgacggtgc tgggcgcggc ggcgggttcg
    20281 ctcgtcggcc tcgtgaccgg ctactaccgc gggcgcgtgg accgcgtggt gaccaagctc
    20341 atcgacgtgc agctcgcttt cccgttcgtg ctgctcgcca tcgccgtgat cgcggtcgcg
    20401 ggcccgagcg tcgccgtgct ggtcgtcgtg ctggccatcg gcgcctgggt cggccatgcc
    20461 cgcatcgtgc gcgggctcgt gctgagcctc cgggagcgcg agtacgtgca ggccgcgcac
    20521 gcgctgggcg cggcgacccc gcgcatcctc ttccgccacc tcctgccgga gatcctctcg
    20581 gtcatcgtcg tgctggccac gttcgacgtg gggcgcatca tcatcctcga gtcgacgctg
    20641 agcttcctcg ggctcggcgt gcagccgccg acgccgtcgt ggggctcgga cctgcgcgat
    20701 gcggccgcct acgtgcgtca ggcgtggtgg acggcggcgt ttcccgggct ggcgatcatg
    20761 gtcgtggtgc tgggactgaa cctggtcggc gatgcgctgc gcgacgcgct ggatccgcgg
    20821 acggtggcgc cgcgcggtcg cgcctgaccg gccgcggagc tcgaaaggag acgacatggt
    20881 gacgacgtgg acccgctggg ccctctgcct cgtgctcgcc gcggcgctgg cggcggcggc
    20941 gccgccagcc ggcgcgcagc ggggcacgca gatcacgatc tcgcaacctt ccgacgccac
    21001 gacgatggac cccgcgcgct ccacccaggt cctcaccgtc aactatttcg tcaacctcta
    21061 cgacacgctc acgcgctggg acacgtcgct caagctccag ccggggctcg ccacggcgtg
    21121 gaagagcgtc aacgacacga cctgggacat cacgctgcgc ccgggcgtga agttccacga
    21181 cggctccccg ctcaccgccg cggacgtcaa ggccgtctac gagcgggcgc ggacgccggg
    21241 caagagcgtg gtggccgccg gcttcgccac gattgcggcg atcgaggtca ccggtccgac
    21301 ctcgctccgc atcgtgacga agaaacccga ccccctgatg cccgtgcgca tggcacagat
    21361 gggcggctac gtgtaccccg cccgcttcgc caacgacgag ggcgccaagg agctggcccg
    21421 caagcccgtc gggtccggcg cctaccggtt cgtgtcgtgg acgaaggacg accagctcgt
    21481 gatggaggcg aaccgcgact ggtggggctg gagcgacaag ccgccgacca tcgaccgcgt
    21541 cgtgtggaag cccattcccg acgacttcgc gcggctcagc gcgctgcagc gcgccgaggt
    21601 ggacgtcatc acgaacgtcc cgcccgacca gatgaagatc gtccgcacgc tcaccgtgcc
    21661 ggcgacgcgc acggtcacct tctggatcaa caccacgcag ccgccggcgt ccgacgcgcg
    21721 cgtgcggcag gcgctgcact acgcgatgga tgtgcagtcg atcgtcaaga acctctacgc
    21781 ggggcagggc aagcccttca gcggcggcct ggccgacacg gacttcggct acaaccccga
    21841 cctcaagccg tatccctacg acccggagcg ggcgaagaag ctgctcgccg aggctggcca
    21901 cgccggcggc atcgacgtga cgctctacgc cggcagcggc accatggtca acgacaagca
    21961 gctcctcgag gccatcgccg acatgtggtc gaaggtcggc atccgcgccc ggatcgagat
    22021 gatggagatg gcggcgcggc agaagatgaa caacgagcgg acggtgccgc ccaacgggct
    22081 catgctgatc aacccgcaat ccacgctgct cgacgccgac ggcagcctct ggcggctctt
    22141 ccacccgacc ggcttcggcg gcaagtactg ggcggggaac cagcccggcc agcgcttcca
    22201 cgacctgatg gagcaggggc gctacacgct cgatccgaag aagcgcaagg cgctctacac
    22261 cgaggccacg cggatcatcc acgacgagaa gccgtggctc gagctgttcc aggaggtcgt
    22321 catctacggc gtctccaagc ggacgagctt caagccccgc gccgactacc ggttgatcgt
    22381 cgcggagatg acggtcggca agtagcgtgc ggcgtctcct gctcgccgtc gtgctcgcac
    22441 cccccgcgct gggcgccgcc gctgcccagc gcgacgaggc cccgagcatg taccacatcc
    22501 acgggctcgc gttcgccggc gcggatcccg aggtcctcct cgtcgccacg cacacgggac
    22561 tcgtgcggct gcggtcgggc gcgcccctgg aatgggtcgg cgagcaccgc ttcgacctga
    22621 tgggcttcgc cgcgcacccg acccaggcgg ccctcgtgtt cgccagtggg caccccgacg
    22681 tgcagacgta cgcgcgcgag cgcgtcggca acctgggatt gctcgtcagc ggcgacggcg
    22741 gccggacgtg gcggtccgcc gtcctccggg acgaggcgga cttccacgcc ctcgcctaca
    22801 gtccggccga tggcggacga ctctacggct ggagcgtggt cgggcatgcg ggactgtacc
    22861 ggatctcgac cagtgactgg agcgtccagc gattgccggc gcccgggctg gaggacgtgc
    22921 tcgggctcgc cgccagcccc gacgccgccg gcacgctgtt cgccggcacg acgcaggggc
    22981 tgatggcgag ccgcgacggt ggcgcgacgt ggcgcgccgt cgcgggcttg ccgcgcctgc
    23041 cggtcaccgc ggtcgcgtac gacgtggccg acgccaaggt cgtctacgcg gccctcctgc
    23101 gtggccaggg gaccgtcatg cgaagcccgg acggcggcgt gacgtggcgg cggacgagct
    23161 tcgagaccga gcgggagacg gcggtcgtgg cgctcgccgc cggcccgggc ggccacgtgg
    23221 cggcggccac caccggcgcg gacgtcgtgc ggtcgcgcga cggcggccgg acgtggacga
    23281 agatcctgga gcggggccgc gtgagcgggc cggcgccgga ctgaccggcg ctgcggtgcc
    23341 gaccgcggcg cctcacgcgg cttcgccacg ctggagcacg gcgacgtcga acagcaccgc
    23401 ggcgcgctgc acgaggctcg cgatcacctg gccgtagaac cggggcatct cgttgagacc
    23461 ggactcgggg gccacgaagc gggcgctcgc cggctagccc acgaactggc gggtgaagtc
    23521 gcagtccagc aggggcttca cccacgtgta ggtctcctcg gtcagctcca ccacggtggc
    23581 gccgcgtacc cgaccgtcgg ggccgagcgc gaagcggaac gtcagcccga gccagtcgta
    23641 gatgtcgtgg tgggccagcg cgtagagacc ccacgtcacg tcctcgtcgc tgaccgcggg
    23701 ggcggcgaag ggcacgccgc gctggcggaa gctgacgtcg ctctcgccgc cgcacaggaa
    23761 ctcgccggcc aggatcagcc gggccagcat ataccgaacc cgggctgccg cggttcgtcc
    23821 gcagcggacg gaaagatggt cgtgtagagt gacatcgatg ccgagggccc tgtacgcgcg
    23881 gctggcggtc gtgctcctgg cggccgcgtg gctcgccgcc gcccccgccg ccggggcgac
    23941 gtcgctcgag gaggtcatcc gtggcctcga gtcggcttac agccgcctga ccgacctgcg
    24001 ggccgagttc acccagacgt ccttcaacaa gagcctcaac tccaccatcc ccgccagggg
    24061 cgccgtctac ttgaagaagg gcggcaagct ccgctgggag ttcgccgagc cggcgaagca
    24121 ggagatcgtc tccgacggtg cgaccctctg ggtctacacg ccgtcgctca accaggtgaa
    24181 caccggcccg gcgccggagg cgctggccgg tccggctggc tcg
//
LOCUS       DATIAZ010000129         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1872350, whole genome shotgun sequence.
ACCESSION   DATIAZ010000129 DATIAZ010000000
VERSION     DATIAZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872350"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1177)
                     /locus_tag="VLF19_04505"
     CDS             complement(182..1177)
                     /locus_tag="VLF19_04505"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92547.1"
                     /translation="MATVGRRTFLKVGAAAALAGAPLAARAQGKGKVRLAYLQLGWAA
                     TEIIHKEDLLGKRGWQAEYSIVPGAPGPLLNQLASKNVDAIDMSFALAAKAFEDGVPL
                     KVTGVATAVLGAVIARKDAGLARVEDLKGRKVAAIVGTSTFFDIRALTLKGYGFDLQK
                     DAQIVTATSPPDMVTLLGKKEVDAIIAWQPITDSVVLRGEGVYLAKQIDLWRKATGRP
                     TGFPVHVCYLVHGDFLGKHAAIAGDLNDAQKDAVDIWYKKPDRAKEIVAEVTKLPKNV
                     IDVAHKETVRMLHGLPDEQVDTLIAQLKINKEFGFLKSDIWDSPERIRREFFHRA"
     gene            complement(1205..2584)
                     /locus_tag="VLF19_04510"
     CDS             complement(1205..2584)
                     /locus_tag="VLF19_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HSE92548.1"
                     /translation="MAAADESRTKIVLDERDIPTKWYNIQADLKTPAPPVLHPATGNP
                     IGPQDLAPLFPMELIKQEVSRERWIAIPDEIRDVLRLWRPSPLYRARRLERTLGTPAR
                     IYYKYEGVSPAGSHKPNTAVAQVYYNKQEKVARISTETGAGQWGSALAMACAFFGLQC
                     KVYMVKVSYNQKPYRRIMMETWGAQVVPSPSPDTQAGKKVLEEDPDSPGSLGIAISEA
                     VEDAATRDDTKYSLGSVLNHVLMHQTVIGLEAKKQLERADDEADVVIGCAGGGSNFAG
                     LSFPFAGDKITGKRKKIRIIAVEPSACPSLTKGKYVYDFGDTVGLTPLVKMHTLGHGF
                     VPAPLHAGGLRYHGMAPLVCKLYDEGVIEAVAVPQVATFQAAVQFARTEGIIPAPESA
                     HAVRVAIDEALRCKHDGKKQAILFNLSGHGHFDLGAYEAYLSGRLQDFEYPAEKIEAA
                     LRGVPKVGG"
     gene            complement(2670..>3195)
                     /locus_tag="VLF19_04515"
     CDS             complement(2670..>3195)
                     /locus_tag="VLF19_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSE92549.1"
                     /translation="AGMLAQKQRAREDMPDFAAAVAREKQGNALSQQLAFKEAADNYR
                     AAADLFARAVPPPPAARPKAPPADPQREIRAVIESYAQALERKDLALLQKVSPGLKPD
                     DLRRIRDSFDRSQSLKATLKVETIEVTGDEARARGRREDILVTTDGRTFRNESGFSFR
                     LKRRGDGWGIEALN"
BASE COUNT          431 a         1096 c         1120 g          548 t
ORIGIN      
        1 gggggggggg gggggggggg gggggggggg ggcccgccga cctgtccccc gcgaaccgat
       61 tctcatctca cccgaagatg gacatgacac ccgaaggacg acgcgcaccg cgtcaggggt
      121 cgctggaccc ccgccccccg ccccgacccc accccggccg gcgtctgctg cgagcgtccg
      181 actaggcgcg atggaagaac tcgcggcgga tgcgctcggg gctgtcccag atgtcggact
      241 tgaggaagcc gaactccttg ttgatcttga gctgcgcgat cagcgtatcg acctgctcgt
      301 cgggcaggcc gtgcagcatg cgcacggtct ccttgtgagc cacgtcgatg acgttcttcg
      361 gaagcttcgt gacctcggcg acgatctcct tggcgcggtc gggcttcttg taccagatgt
      421 ccacggcgtc cttctgcgcg tcgttcaggt cgccggcgat ggcggcgtgc ttgcccagga
      481 agtcgccgtg caccaggtag cagacgtgga ccgggaagcc ggtcgggcgc ccggtcgcct
      541 ttcgccagag gtcgatctgc ttggccaggt acacgccttc gccgcgcagc accaccgagt
      601 ccgtgatggg ctgccacgcg atgatcgcgt cgacctcctt cttgcccagc agcgtcacca
      661 tgtcgggcgg cgacgtcgcg gtgacgatct gcgcgtcctt ctgcaggtcg aagccgtagc
      721 ccttgagcgt gagggcccgg atgtcgaaga aggtcgaggt gccgacgatc gcggccacct
      781 tgcgcccctt gaggtcctcc acccgggcga ggcccgcgtc cttccgggcg atgacggcgc
      841 cgagcacggc cgtggccacg ccggtgacct tgagcggcac gccgtcctcg aaggccttgg
      901 cggcgagcgc gaaggacatg tcgatggcgt cgacgttctt ggaggcgagc tggttgagca
      961 gcgggcccgg cgcgccgggc acgatggagt actcggcctg ccagccgcgc ttgccgagca
     1021 ggtcctcctt gtggatgatc tcggtcgccg cccacccgag ctgaaggtac gcgagccgca
     1081 ccttgccctt gccctgcgcg cgcgccgcca gcggcgcgcc ggcgagtgcc gcggccgcgc
     1141 cgaccttcag gaacgtgcga cgtccgaccg tcgccatggc gagcctccct ggatgtgcca
     1201 ccggctagcc cccgaccttc gggacgccgc ggagcgcggc ctcgatcttc tccgcggggt
     1261 actcgaagtc ttgaagtctg ccgctcagat acgcctcgta ggcgccgagg tcgaagtggc
     1321 cgtgcccgga caggttgaag aggatcgcct gcttcttgcc gtcgtgcttg cagcgcagcg
     1381 cctcgtcgat ggcgacgcgc acggcgtgcg cggactcggg cgcgggaatg atgccctcgg
     1441 tacgcgcaaa ctgcaccgcc gcctgaaacg tggcgacctg gggcaccgcg acggcctcga
     1501 tgactccctc gtcgtagagc ttgcagacga gcggcgccat gccgtggtac ctcaggccgc
     1561 cggcgtgcag cggcgcgggc acgaagccgt ggcccagcgt gtgcatcttc accaggggcg
     1621 tcagccccac ggtgtcgccg aagtcgtaga cgtacttgcc cttggtgagg ctggggcagg
     1681 ccgacggctc gacggcgatg atccggatct tcttccgctt gcccgtgatc ttgtcgccgg
     1741 cgaacgggaa ggagagcccg gcgaagttcg agccgccgcc cgcgcagccg atgaccacgt
     1801 ccgcctcgtc gtcggccctc tcgagctgct tcttcgcctc gagcccgatg accgtctgat
     1861 gcatcagcac gtggttgagc acgctgccga gcgagtactt ggtgtcgtcc cgcgtggcgg
     1921 cgtcctcgac ggcctcgctg atcgcgatgc cgagactacc cggcgagtcc ggatcctcct
     1981 cgaggacctt cttgccggcc tgggtgtccg ggctcgggct gggcacgacc tgagcgcccc
     2041 aggtctccat catgatccgc cggtacggct tctgattata ggagaccttg accatgtaga
     2101 ccttgcactg cagcccgaag aacgcgcacg ccatggccag cgccgagccc cactggccgg
     2161 cgccggtctc ggtgctgatg cgcgcgacct tctcctgctt gttgtagtag acctgcgcga
     2221 ccgccgtgtt gggcttgtgg ctgccggcgg ggctcacgcc ctcgtacttg tagtagatcc
     2281 gcgccggcgt gcccagcgtc cgctccaggc gccgcgcccg gtagagcggc gaggggcgcc
     2341 agaggcgcag cacgtcgcgg atctcgtcgg gaatggcgat ccagcgctcg cggctcacct
     2401 cctgcttgat cagctccatc ggaaagagcg gcgccaggtc ttgaggcccg atgggattgc
     2461 ccgtcgcggg gtggagcacc ggcggtgccg gcgtcttgag atcggcctgg atgttgtacc
     2521 acttcgtcgg gatgtcccgt tcgtcgagga cgatcttggt gcggctctcg tcggctgcgg
     2581 ccatggcggg cctccttcga gtcgaaacgg gcgcccggaa acgtgtgccc acgatacaga
     2641 aacgcttcgc gggctgtcaa acgacggcct cagttcagcg cctcgatgcc ccagccgtcg
     2701 ccccggcgct tcagccggaa gctgaagccg gactcgttgc ggaacgtccg tccgtccgtc
     2761 gtgacgagaa tgtcctcgcg ccggccccgg gcccgcgcct cgtcgccggt gacctcgatg
     2821 gtctcgacct tcagggtggc cttgagactc tggctgcggt cgaacgagtc acggatgcgg
     2881 cggagatcgt ccggcttgag tcccgggctc accttctgga ggagggccag gtccttgcgc
     2941 tcgagcgcct gcgcgtagct ctcgatcacg gccctgatct cacgctgcgg gtcggcgggc
     3001 ggcgccttcg gtcgcgccgc cggcggcggc gggaccgcgc gcgcgaacag atcagccgcc
     3061 gcgcgatagt tgtcggccgc ctccttgaac gcgagctgct gggagagggc gttgccctgc
     3121 ttctcgcggg ccacggcggc ggcaaagtcg ggcatgtcct cccgcgcccg ttgcttctgg
     3181 gcgagcatgc cggct
//
LOCUS       DATIAZ010000130        12291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_426609, whole genome shotgun sequence.
ACCESSION   DATIAZ010000130 DATIAZ010000000
VERSION     DATIAZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426609"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..360)
                     /locus_tag="VLF19_04520"
                     /pseudo
     CDS             complement(1..360)
                     /locus_tag="VLF19_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            complement(458..532)
                     /locus_tag="VLF19_04525"
     tRNA            complement(458..532)
                     /locus_tag="VLF19_04525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Val,seq:gac)
     gene            complement(608..1054)
                     /locus_tag="VLF19_04530"
     CDS             complement(608..1054)
                     /locus_tag="VLF19_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HSE92550.1"
                     /translation="MDIRSLGRGGGAGGFRGGRGGIEGEPIPLDSPDAKYSDWLERVR
                     RQIKEKWGYPCVKNPLTGECEYKTATLVVEFGVLRGGQLQFIEVRRPSGFSIYDDYAA
                     NAIKLGAPFPQVPDAMMTRMVRGSTGVAIQATFNYVVSTSLTNVLR"
     gene            complement(1774..2814)
                     /gene="aroF"
                     /locus_tag="VLF19_04535"
     CDS             complement(1774..2814)
                     /gene="aroF"
                     /locus_tag="VLF19_04535"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSE92551.1"
                     /translation="MIIVLKSGASDTEVEDVCRRILAMGYRPHTIRGELRTVIGAVGD
                     DRGKERLRALESLEFVESVTPILQPFKLASREVKSEPTRVRITPAGAPSAPAVEIGGD
                     RVVVMAGPCSVESRDQVLEVAMKVKAAGATVLRGGAFKPRTSPYAFQGLEAEGLKYLA
                     EARRETGLPIVTEVMEPERVDLVAEHADILQIGARNIQNFSLLRRVAEAGKPVLLKRG
                     MATSIQEWLLSAEYVLSGGNPDVILCERGIRTFETTTRFTLDLNAVPVVKKLTHLPVV
                     VDPSHGTGHWEYVEAMAKAGIAAGADGLLIEVHPRPAEALSDGPQSLKPDRFADIMAR
                     LRRVARALDRDL"
     gene            complement(3003..3782)
                     /locus_tag="VLF19_04540"
     CDS             complement(3003..3782)
                     /locus_tag="VLF19_04540"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HSE92552.1"
                     /translation="MTTRRQRQRRSSAGVGWVLASVVLAVLLFIVVWLVVTDSPVIRL
                     LVRLYRDKQFLKQTVTSWGWAAPVVFMAIQALQVIISPFPGEITGPVGGALFGTWWGL
                     FYSSIGLTVGTLTCFGVGRKWGEPLVRPWLSEHNWNRLNFIIEAEGAIICFILYLIPG
                     FPKDIVSYLFGISPMPFWLFALVSTFGRLPGTWISSYFGANVAEQRYLYAVLFLAVVI
                     AICLPLYYYRDRILRRLIRRRRPRGRRQPEGLTRRGGAGVE"
     gene            complement(3779..5194)
                     /locus_tag="VLF19_04545"
     CDS             complement(3779..5194)
                     /locus_tag="VLF19_04545"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSE92553.1"
                     /translation="MPTVRDDAVWLSAVELAARIRSKQLSSVDVTETFLSRIEALNPR
                     LNAFCLVAHDVARRAAREAEIAVMKGEPLGPLHGVPVSIKDVIFTRGMRTTGGSRLFA
                     EAVPEEDAVSVGRLRAAGAVPLGKTNTSEFGHKAVTDNPLFGATRNPWNLKLTPGGSS
                     GGAAAAVAAGLGPIALGTDGGGSVRIPASFCGVFGFKPSRGRVPDHPGFPGWDLLSST
                     GPLARTVRDAALVLDVVAGGDDRDRHSLPRESGSYVEACDGDVKGLHVAWTPDLGYAT
                     VDPRVQALCENAAAEFEALGCHVEVVNPGWENPEEIFGAIMTARFHAHWADQLPAHVA
                     ALDPTLVKFIQRGASVTARDYVLAEERVKGYWSEVLAFLARFDLLLTPTVAVPPFPAG
                     QRPPREIDGQPLSVLGWMPFTYPFNLTGQPAASVPAGFTDDGRPVGLQIVGRRLADRV
                     VLAAAAAYEAACPWSGRRPDP"
     gene            5265..6623
                     /locus_tag="VLF19_04550"
     CDS             5265..6623
                     /locus_tag="VLF19_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92554.1"
                     /translation="MDPGPAPRPRTPLDDAVVRALGSVAATLAPLLAGAPDLDHLLQT
                     RHHGTLVAYAEVDGARRRVLELDRGGTPLAVLRWAERALDAAWLKIPDGRWVAIEPRA
                     SSTAPWGAADRLRTASEPGALGDPLTLFEALDYTAVDRVPTLAEPGRVPPGAGVVVLN
                     LLASLTTDERRERLAYHGPYPGEQLFLALLESFRYEPAVADPLAAFVAGALSWRPDPH
                     ERWFTSDGVYVQWRGRVEKVVWRDRAYRRPDWQGVARHAPLRVRDADGAVRCSLWALG
                     GPLEDHLELSPAGTVVRVWAPSPQAVPPRALPAVAAAGVGAVVAATSAPALAAFVRQE
                     AAALRLTWAHLSGGLVEIEGAEVRLSTRLGERFADRTRAAADEAVRATLGLAALAEIA
                     GLVGDTLRARAQARAAALPAADQEALLAAPPAAAGAGDARAITAAVGALLDDAAARSA
                     SG"
     gene            complement(6679..7923)
                     /locus_tag="VLF19_04555"
     CDS             complement(6679..7923)
                     /locus_tag="VLF19_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92555.1"
                     /translation="MKGKKRPPPVAAPPRRAKIELLVRDDDGFELRQVSAAVAWIRMR
                     PQRLAENKYELAEVTSAVDKQRRFILKNAATERYLMLSEAERFLWERMDGRTSLQDLA
                     TAYVLQYGEFDFELIPTLIRKLQRAQLLTLTPASLLRRALQRNLRRRVLQTMEHALTA
                     LERINVSSRRVHPVFDRLYRWGGFLLFTPAALVVCLALAGLGLVAGVRLWREADEIAR
                     GLGAHPFAALLVVKLVFLGTLALHQIVHALALVHYGRRVREFGFTFLHGFVPTFYVDV
                     TDIFMASRRARIVTAVSGTLAHLMLGTLWFLIALGASEGGFVQAFAAASGMIQWQAFI
                     IALYPFCFIEMDGYHVLVDVLGLPTLKQDALAYVGSLFRGAPRSRWGWEAGIWVLYVT
                     LSAASVAAFVAFNVWMIAHAVA"
     gene            complement(7923..8423)
                     /locus_tag="VLF19_04560"
     CDS             complement(7923..8423)
                     /locus_tag="VLF19_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSE92556.1"
                     /translation="MPRAAETVAFLKTVRLFEEFAEPDLLALSSRLRQRSLRRGQVLF
                     REGDAGDEMFVVQQGSIIISKVVTGRVEQVLSRESAGSFFGEMSLFDHSPRSATVQAE
                     SDVELLVLDRDNLTLLIEVNPRAAAAFFHALVHVFIERLRASGKLIAEVTRWGLEATG
                     LDMEGR"
     gene            complement(8438..8656)
                     /locus_tag="VLF19_04565"
     CDS             complement(8438..8656)
                     /locus_tag="VLF19_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92557.1"
                     /translation="MAEHLPELVGRAMIDPDFLAELQSAPEAVLARYELTELERAAVR
                     TAVARLAQTPAHRRAQELGTALLRRVAT"
     gene            complement(8961..9212)
                     /locus_tag="VLF19_04570"
     CDS             complement(8961..9212)
                     /locus_tag="VLF19_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92558.1"
                     /translation="MARPVADRCGVCGAQIRGKATVTWVDDRVVHVVCYTPGLGKKTR
                     KPAASGTLGLRDGRLFAPSPGSTVEAEPVDSLKSRATGA"
     gene            complement(9727..9805)
                     /locus_tag="VLF19_04575"
     tRNA            complement(9727..9805)
                     /locus_tag="VLF19_04575"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9767..9769),aa:Glu,seq:ttc)
     gene            complement(9857..10594)
                     /locus_tag="VLF19_04580"
     CDS             complement(9857..10594)
                     /locus_tag="VLF19_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92559.1"
                     /translation="MKTLALFLTALAVVGCTSTAPRSASTGSPASASSGGAAAPSTFT
                     GEVWVWDEQASTVTLRQATRTIRVKVTPDQLAGLRLYQVATIRGELAPAEIETVAVPP
                     GALVPRGDADQVEILGTVRGFDPVGKVAVASPQGPLTLWIAQPGDAPFRSGDRVTVRM
                     RVQPFDVAPAHPGQPPRARDAEPAASIGSEPGEYAAVKGRVTAIAPDGRLTLESPRGP
                     ITVAVPNVARYRVGDTVEAHTSVHLAR"
     gene            10675..11088
                     /locus_tag="VLF19_04585"
     CDS             10675..11088
                     /locus_tag="VLF19_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HSE92560.1"
                     /translation="MTDDTVPPELARTIENDPWARTLGVQFLELKRGYCRVALQLGPH
                     MVIFQGAPHGGVIFSLADIAFGAACNSHGEPAVALSVTVGYLTAVRPDARLVAESRAR
                     RQGRRAGFYDVTVTSDDGRLVATVHCVAHRVGGEA"
     gene            complement(11113..11490)
                     /locus_tag="VLF19_04590"
     CDS             complement(11113..11490)
                     /locus_tag="VLF19_04590"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HSE92561.1"
                     /translation="MTTVGRRLGCGLVTAALLALMAVSVAAQGPFRITYDVDRSSPGR
                     TRIAGQVFNDTPTDVVDVYVTAEAVDGNGKVVARGISFVSPAISRGGSAPFEAAVPAP
                     PSATNFRVRVSSYRQGLGASQAP"
     gene            complement(11490..11768)
                     /locus_tag="VLF19_04595"
     CDS             complement(11490..11768)
                     /locus_tag="VLF19_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92562.1"
                     /translation="MPFDSRLAEASPVDRAGTLALARGARRLLVKAGAETVRVDARAE
                     SLAEADAVRYLLHEDGFLRVPVLVVGDLLVRGFTDDLYREALGVGGGG"
     gene            complement(11874..>12291)
                     /locus_tag="VLF19_04600"
     CDS             complement(11874..>12291)
                     /locus_tag="VLF19_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HSE92563.1"
                     /translation="LNRALAGGGGTTAVHICFGYAAIIHQRPSGYSFLPELAGCGCAQ
                     VSVETAQSKLDCAVLATLAGKQIMVGCLDLNDPAVETPATVVERVKRALPYVAPENVV
                     LAPDCGMKYLPRAIAFGKMAAMVAAARMLRAEFGAR"
BASE COUNT         1832 a         4393 c         4385 g         1681 t
ORIGIN      
        1 ctggcccgtg gacggtacgt ggggcccgcg gcagagatcg acgaactcgc cctgctggta
       61 gagcgacacg tgcggcgcgt cgaggccttc gaggatctcc accttgaacc gctccccgcg
      121 ctcgcggaag aacgcgatgg cctcggcgcg cggcatctcc cggcgctcga acgcgtgatc
      181 ggcggcggcg atctcgcgca tcgtcgcctc gacgcgcgcc agctcctccg gcgtgaacgg
      241 ctcggctttc gcgaagtcgt agtagaagcc gtcctcgatg gccggaccga tggccagctt
      301 cacgtcgggg aacagacgct tcaccgcctg cgccatgacg tgcgcggtgg agtggcggag
      361 cgtggagagc ggcgccgggt cgcagtcgaa ctcgccggtc agcgtgaggt gtcgctcggc
      421 ttcagcctcg gccatacact cctgaagagg ttgggattgg taggctcggg cggtttcgaa
      481 ccgccgacct cctctgcgtc agagaggcgc tctcccactg agctacgagc ctatcccatg
      541 aagtaccgca catgctacat ggccccttga agacgtgtca agcgaaagcc ctgagaatac
      601 aggactttca ccggaggacg ttggtcaggg acgtgctgac gacgtagttg aaggtggcct
      661 ggatggccac gcccgtgctg ccccgcacca tcctcgtcat catcgcgtcc gggacttgcg
      721 ggaacggcgc gcccagcttg atcgcgttgg ccgcgtagtc gtcgtagatc gagaagcccg
      781 agggacgccg cacctcgatg aactggagct ggccacccct gaggacgccg aactccacca
      841 ccagcgtcgc ggtcttgtat tcgcactccc cggtcagcgg gttcttcacg cagggataac
      901 cccacttctc cttgatctgc cggcggacac gctcgagcca gtcgctgtac ttcgcgtccg
      961 gcgagtcgag cgggatcggc tcgccttcga tccccccacg gccgccacga aacccaccgg
     1021 cgcctccccc ccgtccgagc gagcggatgt ccaccggtgg tgcctcgcgc gagggcggta
     1081 cggacgccac ctggggcccg ggtggagcgt cgccggcagc gggcgccgcc ggctgtgctg
     1141 gcgcccgggc gctcggttcc tcgggcgcgg gcgaagatgg cgcgagcggc cgagccggct
     1201 ccggcgctgg ctccacctgt cgcggcggct ccaggggtcg gggagactcc atcggctcct
     1261 gaggatccgc cgggagcgga ggatcaggct cgcgcggctg cggtgccggc ttcgggggac
     1321 ggctcgcgac cgacggcggc ggcgtctccg ggggacggct ggcgaccacc ggcggcgcgg
     1381 gtggggacgg cgtcgggggt attgcggtca cgggcggcgc ggctggtcgc ggcgcgggtc
     1441 tggcggccgt gcgggccggc tcgggagcct ttggcctggg cggcggcgaa ccggcgggcg
     1501 gccccggtgt cctggccgcc gggttgccgg cgggcgcagg accgggcagc tcgggcaact
     1561 ccacgaagag cggctccccc cgcttgacgg cgatcggaga tgggggggcc gcgaagaaga
     1621 cggccaccac caggaggacc gcgtggatcc ccagcgagcc cacgattccg cgcatcggcc
     1681 ggcgccccag gaacttccgg acccgctcca gcatctcgac gagaattata ggcccgaagg
     1741 cgagattagg agggccatgt cggggtcctc atctcacaga tcgcgatcga gggcccgcgc
     1801 caccctgcgg agccgcgcca tgatgtcggc gaagcgatcg ggcttgagcg actggggacc
     1861 gtccgagagc gcctccgccg ggcgaggatg cacctcgatg aggaggccgt cggccccggc
     1921 ggcgattcca gccttggcca tcgcctccac gtactcccag tgtcctgtcc cgtgactcgg
     1981 atcgaccacg accggtaggt gggtgagctt cttgaccacg ggcactgcgt tcaggtccag
     2041 ggtgaaacgg gtcgtggtct cgaaggtccg gatgccccgc tcgcacagga tcacgtcggg
     2101 attgccgccg ctcagcacgt actccgcgga cagcagccac tcctggatcg acgtcgccat
     2161 gccccgcttg agcagcaccg gcttgccggc ctccgccacc cggcgcagca gcgagaagtt
     2221 ctggatgttg cgcgccccga tctggaggat gtcggcgtgc tcggccacca ggtccacccg
     2281 ttcgggctcc atcacctcgg tcacgatcgg taaccccgtc tcgcgcctgg cctcggcgag
     2341 gtacttgagc ccttcggcct cgagcccctg gaacgcgtag ggcgaggtcc gcggcttgaa
     2401 cgcgccgccg cgcaggaccg tcgcacccgc ggccttcacc ttcatcgcca cctcgagcac
     2461 ctggtcgcgc gactccaccg agcaggggcc ggccatgacg accacccggt cgccgccgat
     2521 ctcgaccgcg ggcgccgacg gcgcgcccgc cggcgtgatc cgcacgcggg tcggctcgct
     2581 cttcacctcg cgactcgcga gcttgaaggg ctgaaggatc ggcgtgaccg actccacgaa
     2641 ctcgagcgac tccagcgcgc gcaaccgctc cttgccgcgg tcgtcaccga cggcgccgat
     2701 gacggtgcgc agctcgccgc ggatcgtatg cggccggtac cccatcgcca ggatgcggcg
     2761 acagacgtcc tcgacctcgg tgtcgctggc cccggacttc aacacgatga tcatcacggc
     2821 ctccttgacg aaaaaaggcc gcgggccttc cgggcccgcg gcctcgttcg tcgtcggcgc
     2881 cgcgggccct cggtccgccc gcggctccct gctatctcac gtcagagcgc ctgggcagac
     2941 cccggaaagg ggtaagagaa gaagccgaag cccgtccagg tcgtgtgcgt catggccgac
     3001 ggctactcta caccagcccc gccccggcgc gtcaagccct ccggctgccg gcgcccgcgg
     3061 gggcgccgcc ggcgaatcag ccggcgcagg atgcggtccc ggtagtagta gagcggcagg
     3121 cagatggcga tcacgaccgc caggaacaac accgcgtaga gataacgctg ctccgccacg
     3181 ttggcgccga agtacgagga gatccacgtg cccggcaggc ggccgaacgt cgagaccagc
     3241 gcgaacagcc agaagggcat cgggctgatg ccgaagaggt aggagacgat gtccttgggg
     3301 aaccccggaa tgaggtacag gatgaagcag atgatggcgc cctcggcctc gatgatgaag
     3361 ttcagccggt tccagttgtg ctcgctcagc cacggtctga cgagcggctc cccccacttg
     3421 cgtccgaccc caaagcacgt gagcgtgccg accgtcaggc cgatcgacga atagaagagg
     3481 ccccaccagg tgccgaacag cgcgccgccg accgggcccg tgatctcgcc cgggaagggc
     3541 gagatgatga cctgcagcgc ctggatcgcc atgaacacga ccggggccgc ccacccccac
     3601 gacgtgaccg tctgcttgag gaactgcttg tcgcgataga gacgaacgag caggcggatg
     3661 acgggcgagt ccgtcacgac gagccacacg acgatgaaca ggaggacggc gaggacgacc
     3721 gaggccagga cccaaccgac gccggcgctc gatcggcgct gtcgctggcg acgtgtcgtc
     3781 acggatccgg gcggcgcccg ctccacgggc acgccgcctc gtaggccgcc gcggcggcca
     3841 gcacgacccg gtcggcgagg cgacggccga cgatctgcag gccgaccggc cttccgtcgt
     3901 ccgtgaagcc ggcgggcacg ctcgccgccg gctggcccgt caggttgaac gggtacgtga
     3961 aaggcatcca gccgaggacc gacagcggct ggccgtcgat ctctcgcggc gggcgctggc
     4021 ccgcggggaa cggaggcacc gccacggtcg gggtgaggag caggtcgaag cgtgcgagga
     4081 aggccagcac ctcgctccag tagcccttca cgcgctcttc cgccagcacg taatcgcgcg
     4141 cggtcaccga ggcgccgcgc tggatgaact tcacgagcgt gggatcgagg gcggccacgt
     4201 gggcggggag ttgatcggcc cagtgcgcgt ggaaccgcgc ggtcatgatc gcgccgaaga
     4261 tctcctcggg gttctcccag cccggattca ccacctcgac gtggcagccg agcgcctcga
     4321 actccgcggc ggcgttctcg cagagcgcct gcacgcgcgg gtcgaccgtg gcgtagccca
     4381 ggtcgggcgt ccaggccacg tgcagaccct tgacgtcgcc gtcgcacgcc tcgacgtacg
     4441 agccgctctc gcgcggcagc gagtggcggt cccggtcatc gccgccggcg acgacgtcga
     4501 gcacgagcgc cgcgtcgcgc accgtgcggg cgagcggccc ggtggacgac agcaggtccc
     4561 agcccggaaa ccccgggtgg tcggggacgc gcccgcgcga gggcttgaac ccgaagacgc
     4621 cgcagaacga ggcagggatg cggacggacc cgccgccgtc ggtgcccagc gcgatcgggc
     4681 cgagcccggc ggccaccgcc gcggcagcgc caccgctcga gccgccgggg gtcagcttca
     4741 ggttccaggg attgcgcgtg gcgccgaaca gcgggttgtc ggtcacggcc ttgtggccga
     4801 actccgacgt gttcgtcttg ccgagcggca ccgcgccggc ggcccgcagg cggccgacgc
     4861 tcaccgcgtc ctcttccggc accgcctcgg cgaacagccg cgagccgccg gtggtgcgca
     4921 tccctcgcgt gaagatcacg tctttgatcg agacgggaac gccgtgcagc ggccccagcg
     4981 gctcgccctt catgaccgcg atctcggctt cgcgcgccgc gcggcgggcc acgtcgtgcg
     5041 cgacgaggca gaaggcgttc agccgcgggt tcagcgcctc gatgcgcgac aggaaggtct
     5101 cggtgacgtc gacggacgag agctgcttcg agcggatccg cgcggccagc tccactgccg
     5161 acagccagac cgcgtcgtca cgcaccgtcg gcaccggatc tcatcgggcg cgacatcgag
     5221 agatggccgg agggagacac tcgtgcattg tatcattctg caccgtggac cccgggccgg
     5281 cgccccgccc gcgaacgcca ctggacgacg ccgtcgtccg ggccctcggc agcgtcgccg
     5341 ccacgctcgc gccactgctc gcgggcgcgc ctgacctcga tcatctgctg caaacccgcc
     5401 accacggaac gctcgtggcc tacgccgaag tggacggcgc ccgtcggcgg gtgctcgagc
     5461 tggaccgcgg cggcacgccg ctggccgtgc tccggtgggc agagcgcgcg ctcgacgccg
     5521 cctggctcaa gatccccgac gggcgctggg tggcgatcga gccccgggca tcgtcgaccg
     5581 cgccctgggg cgccgccgac cggctccgga cggcgagcga acccggcgcg ctcggtgacc
     5641 cgctgacgct cttcgaggcg ctcgactaca ccgcggtgga ccgggtgccg acgctcgccg
     5701 agcccggacg cgtgccaccg ggcgccgggg tggtcgtgct gaacctgctg gcctcgctca
     5761 cgacggacga gcggcgcgag cggctcgcgt accacggtcc ctatcccggc gagcagctct
     5821 tcctcgcgct cctcgagtcg ttccgctacg agccggcggt cgcggatccg ctcgccgcgt
     5881 tcgtggccgg cgcgttgtcc tggcggccgg atccgcacga gcgctggttc acgtccgacg
     5941 gcgtgtacgt gcagtggcgt ggccgcgtcg agaaggtcgt ctggcgtgac cgcgcctatc
     6001 gtcggccgga ctggcagggt gtggcccgcc acgccccgct ccgcgtgcga gacgcggacg
     6061 gcgcggtgcg gtgctccctc tgggcgctcg gcggccccct cgaggatcac ctcgagctct
     6121 ccccggccgg cacggtcgtc cgcgtgtggg cgccgtcccc gcaggccgtg ccgccgcgcg
     6181 cgctgcctgc cgtggccgcc gccggcgtcg gcgccgtcgt ggcggccacc agcgctcccg
     6241 cgctcgccgc attcgtgcgc caggaagcgg cggcgctccg gctgacgtgg gcgcacctgt
     6301 cgggcgggct cgtcgagatc gagggcgcgg aggtgcggct gtccacccgg ctcggcgagc
     6361 gcttcgccga ccgcacgagg gcggccgccg acgaggccgt gcgcgccacg ctcgggctgg
     6421 cggcgctcgc ggagatcgcg gggctcgtgg gggacacgct gcgagcgcgc gcgcaggcgc
     6481 gagccgcggc cctgccagcc gccgatcagg aagcgctgct ggccgcgccg ccggcagcgg
     6541 ccggagccgg cgacgcccgc gccatcaccg cggcagtggg agcgctcctc gacgacgcgg
     6601 cggcgcggag cgcgtcaggg tgagccgttc gcgcgccggc tccgccggcg cgatcctgtc
     6661 gaggccgcct ccgacgcctt acgccaccgc gtgcgcgatc atccagacgt tgaacgccac
     6721 gaatgccgcg accgacgcgg ccgacagcgt cacgtacaac acccagatcc cggcctccca
     6781 cccccagcgg gagcgcggcg cgccacggaa cagcgagccg acgtacgcga gggcgtcctg
     6841 cttgagcgtg ggcaacccga ggacgtccac cagcacgtgg tagccgtcca tctcgatgaa
     6901 gcagaaaggg tagagcgcga tgatgaaggc ctgccactgg atcatgccgg aggcggccgc
     6961 gaacgcctgc acgaagccgc cttccgaggc gcccagcgcg atcaggaacc acagtgtccc
     7021 cagcatcagg tgcgccagcg tccccgagac cgcggtgacg atgcgcgcgc ggcggctcgc
     7081 catgaagatg tccgtcacgt ccacgtagaa ggtcggcacg aacccgtgca ggaacgtgaa
     7141 gccgaactcg cgcacgcgcc gcccgtagtg cacgagcgcc agggcgtgca cgatctggtg
     7201 cagggccagc gtgcccagga agacgagctt gacgaccagc agcgcagcga agggatgcgc
     7261 gcccaggccg cgtgcgatct cgtcggcctc gcgccacagc cgcacgcccg ccacgagacc
     7321 gagcccggcc agcgcgagac acaccaccag ggccgccggc gtgaacagca ggaagccccc
     7381 ccaccggtag agccggtcga agaccggatg caccctccgg ctcgagacgt tgatccgctc
     7441 cagcgccgtg agcgcgtgct ccatcgtctg cagcacgcgc ctgcggaggt tgcgctgcag
     7501 cgcccggcgc aggagcgagg ccggcgtcag cgtgagcagc tgggcgcgct gcagcttgcg
     7561 gatgagcgtg gggatgagct cgaagtcgaa ctccccgtac tggagcacgt acgcggtcgc
     7621 gagatcttgc agcgacgtcc gcccgtccat ccgctcccag aggaagcgct ccgcctcgga
     7681 gagcatcagg tagcgctcgg tggcggcgtt cttgaggatg aaccggcgct gcttgtcgac
     7741 cgccgaggtc acctcggcca gctcgtactt gttctcggcc agccgctgcg gccgcatcct
     7801 gatccaggcc accgcggcgc tgacctgccg cagctcgaag ccgtcgtcgt cgcgaacgag
     7861 caactcgatc ttggcgcgcc gcggcggggc cgcgactggc ggcgggcgct tcttgccctt
     7921 catcaccggc cttccatgtc gaggcccgtg gcctcgagcc cccagcgcgt gacctcggcg
     7981 atgagcttgc ccgaggcgcg cagacgctcg atgaagacgt ggacgagcgc gtggaagaag
     8041 gcggcggcgg cccgcgggtt gacctcgatg agcagcgtga ggttgtcacg atccaggacc
     8101 aggagctcga cgtcgctctc ggcctggacc gtcgcggacc gcggcgagtg gtcgaacagg
     8161 ctcatctcgc cgaagaagct gccggccgac tcacggctca gcacctgctc gacccggccc
     8221 gtcaccacct tcgagatgat gatggagccc tgctggacga cgaacatctc gtcgcccgcg
     8281 tccccttccc ggaacagcac ctgcccgcgg cgcagcgagc gctggcggag gcgggaggac
     8341 agggcgagca ggtcgggctc ggcgaactcc tcgaacagcc gcacggtctt gagaaaagcg
     8401 accgtttcgg cggccctggg cacggcgcgc cccgcgctca ggtggcgacg cgacggagga
     8461 gagctgtccc cagctcctgc gcccgacggt gcgcgggtgt ctgggccaac cgggccacgg
     8521 cggtccggac cgcggcgcgc tccagctcgg tgagctcgta ccgggcgagg acggcttccg
     8581 gcgcgctctg cagctcggcg aggaagtcgg ggtcgatcat cgcgcgaccg acgagctcgg
     8641 gaaggtgttc tgccatggcc gctcgatgat accacgtccc gtggggcgcc ctgaatgagg
     8701 cgcgatcaca ccttccttgc tcccgtggga aacccgcgca tcgctcgcct ttctccacca
     8761 cgaatgcgat ctggaagatg cgagcgccgc cagcgaagga ccccgacggt gccgatccgg
     8821 cggccgcggc ccctccttga gacgtgcggc ccgggcggag catgttgcga acggtggtcg
     8881 gtagcgatgg tcggtggtga tcggtgacgg tcggtggtcg tcggtggtgg tgctcggcga
     8941 gcagtcgatg gtgccgatcg tcaggcgccg gtggcccgcg atttcagtga atcgacgggt
     9001 tcggcctcga cggtggaacc cggggaggga gcaaacaacc ggccgtccct cagccccaaa
     9061 gtgcccgacg ccgcgggctt acgcgtcttc ttgcccaggc cgggcgtgta gcagaccaca
     9121 tggacgacgc ggtcatcgac ccaggtgacg gtggccttgc cgcggatctg cgctccgcac
     9181 accccgcaac gatcggcgac tggacgcgcc acgggcggtg acctaggcaa ccagcgtgcc
     9241 gaagctggcg agcacgtccg gccggatccg ggcggccgcc cgtcgtgatg agacagcgtc
     9301 cgcaagatcg cgaacgacgg acagaaacag gtcgcgacgc tcggctctcg ggcctgccat
     9361 ttgatctcga acgccgacct gagcctcggg gcgagcaccc ggcgccctgc ccgttcttcc
     9421 ctggagttgc aagaccgtcc caccatttct tccccggcga cccggccgat aggacggatg
     9481 ggcaagcgcg ccagtcgcga aacgccaggg gctcggaaag gcgtcggcgc tgctgccgag
     9541 ctggaggacg cttccctggc ggaccatagg ccttcctggg cttgcctggg ccttcttggc
     9601 cgcgaggtaa acgagttgcc caccgctgtg gttcctctct actcctggct ctcttggtgg
     9661 ccgatggaga ggactctggc cgttttctcg ctcagccggt gggcgatggc caagtagagg
     9721 gctgggtggt gaccccggcg ggattcgaac ccgcgatctc gaccttgaaa gggtcgcgtc
     9781 cttggccagg ctagacgacg gggtcagcca cgccagtata ctctggcgcc cgggggtgga
     9841 aagctgcgcc tgccgctcag cgcgcgaggt ggacggaggt gtgcgcctcg acggtgtcac
     9901 cgacccggta gcgggccaca ttcggcaccg cgacggtgat cgggccgcgc ggtgactcga
     9961 gcgtgagccg tccgtcgggc gcgatcgcgg tgacccgacc cttcaccgct gcgtactcac
    10021 ccggctcgct gccgatggac gccgcgggct ccgcatcgcg cgcgcgcggc ggctggcccg
    10081 gatgggcggg ggccacgtcg aacggctgca cgcgcatgcg cacggtcacg cgatcgccgc
    10141 tccggaacgg cgcgtcgccg ggctgggcga tccacagcgt cagcggtccc tggggcgacg
    10201 cgaccgcgac cttaccgacg gggtcgaagc ccctcacggt gccgaggatc tcgacctgat
    10261 cggcgtcccc tcgtggcacg agggcgcccg gcgggacggc gaccgtctcg atctcggcgg
    10321 gcgccagctc gccgcgaatc gtggccacct ggtagagccg gaggccggca agctgatccg
    10381 gggtcacctt gacgcggatg gtgcgcgtgg cttgccggag cgtgaccgtg ctggcctgct
    10441 cgtcccatac ccacacctca cccgtgaacg ttgacggggc ggcggccccg ccggatgacg
    10501 ccgaggccgg gctgccggtc gaggcagacc gcggggcggt actggtacag ccgaccacag
    10561 cgagggcggt caggaacaac gcgagcgtct tcatgggagc cctcctcgag gagaatcgtc
    10621 ctccccgatc aagagcgatg ccagcgagaa ccgcatcggc gatactcgag cggcatgacc
    10681 gacgacaccg tcccgcccga gctcgcccgg accatcgaga acgacccctg ggcccggacg
    10741 ctcggtgtgc agttcctgga gctgaagcgc ggctactgcc gggtcgcgct ccagctcggg
    10801 ccgcacatgg tgatcttcca gggggcaccg cacggaggcg tcatcttctc gctggccgac
    10861 atcgcgttcg gcgccgcgtg caattctcac ggcgagccgg ccgtggcgct cagcgtgacg
    10921 gtcggctatc tcacggcggt gcggcccgat gcacggctcg tcgccgagag ccgcgcgcgg
    10981 cggcagggac gccgggcagg gttctacgac gtcacggtca ccagtgacga cggcaggctc
    11041 gtggcaacgg tgcattgcgt cgcccaccgc gtcggtggcg aggcgtgaag gcaagcgccg
    11101 tcccggccgt cctcaaggcg cctgactggc cccgagtccc tgccgatagc tgctgacacg
    11161 gacccggaag ttcgtcgcgc tcggcggcgc gggcacggcc gcctcgaacg gggcgctgcc
    11221 cccccgggag atggcggggc tgacgaagct gataccgcgt gcgacgacct tgccgttgcc
    11281 gtccaccgcc tccgcggtca cgtagacgtc gacgacgtcg gtcggggtgt cgttgaacac
    11341 ctgcccggcg atgcgcgtgc gtcccggtga gctgcgatcg acgtcgtacg tgatgcggaa
    11401 ggggccctgc gccgccacgc tcaccgccat cagggcgagc agcgccgccg tgacgagccc
    11461 gcacccgagg cgcctgccga ccgtcgtcat cacccgcctc ccccgacccc gagagcttcg
    11521 cgatagaggt cgtccgtgaa gccccggacg agcagatcgc ccacgaccag gaccggcacg
    11581 cgcaagaagc cgtcttcgtg cagcagatat cggacggcgt cggcctcggc cagcgattct
    11641 gcgcgcgcgt ccacgcgcac ggtctcggcc ccggccttca ccaggagccg ccgggcgccc
    11701 cgcgcgagcg ccagggtgcc ggcgcggtcc accgggctcg cctcggccag ccggctgtcg
    11761 aacggcacgc catgacgcga gaggaactct ctcgcgcgcg tgcaggtggg tcagccgtcc
    11821 ttgttgtaga ggatggcccg cggcgccacg ccggcgatag taccaccccg gcttcagcgc
    11881 gcgccgaact cggctcgcag catccgcgcg gccgcgacca tggccgccat cttgccgaag
    11941 gcgatcgcgc gcggaaggta cttcatgccg cagtccggag ccagcacgac gttctcgggc
    12001 gccacgtacg gcagcgcacg cttgacgcgc tcgaccacgg tcgccggggt ctcgacggcc
    12061 ggatcgttca ggtcgaggca gcctaccatg atctgcttgc ccgcgagcgt cgccagcacc
    12121 gcgcagtcga gcttcgactg cgccgtctcc accgacacct gcgcgcagcc gcacccggcc
    12181 agctcgggca ggaacgagta gcccgacggc cgctggtgga tgatcgcggc gtagccgaag
    12241 cagatgtgga ccgccgtggt gccgccgccg cccgcgagcg cgcggttcag c
//
LOCUS       DATIAZ010000131         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1742987, whole genome shotgun sequence.
ACCESSION   DATIAZ010000131 DATIAZ010000000
VERSION     DATIAZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742987"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2023
                     /locus_tag="VLF19_04605"
     CDS             <1..2023
                     /locus_tag="VLF19_04605"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSE92564.1"
                     /translation="RPTMWDVDFRTAVAQAELEDREVRGALHRLRFGIHDDGPLLIAT
                     TRPELLAACVAVLVHPEDSRYRRLSGFHAITPLFGARVPVLADAKVDPEKGTGAVMVC
                     TFGDATDVEWWREYELPLRQVIDPAGRLAPVTFGSPGWESADPDRANRAYRQLAGLDV
                     GKARTRVVELLREAGALDGEPEPLTHTVKFFEKGDRPLELIPARQWFVRLLDHRAELI
                     EQGERIEWHPAFMRARYRNWVDGLNQDWCISRQRYFGVPFPVWYPLDSQGQPQYDRPI
                     LAPAAMLPVDPLVQAPPGHDESQRGTPGGFIGDPDVQDTWATSSLSPQIHSRWELDPE
                     RHRSLFPMDVRPQSHEIIRTWAFYTIAKAWLHHRDVPWHHAVISGWVLDPDRKKMSKS
                     KGNVITPLHLLDAYSADGVRYWAARARPGADTAYDESVFKIGKRLVTKLSNAGRFVLG
                     CLGEVDDRRLAPDAITTELDRAFASRLGEVVSRATEAYEDFDWAAALEGVEGFFWAEL
                     CDDYLEIVKTRAYREALDAGRLSALATLRLALSVVLRLFAPVLPTITEELWSRRFATP
                     DGRTRSIHTSPWPTDTELASITAPEHAGAFEAARAIVGAVRRVKGSAKVSLRWPVASI
                     RATGGSAEIAAARSVLDDIRAAGVIREIAFEPGQDGWAFDVRLADSARG"
     gene            complement(2036..>2261)
                     /locus_tag="VLF19_04610"
     CDS             complement(2036..>2261)
                     /locus_tag="VLF19_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSE92565.1"
                     /translation="QGISADLLPHVFERFRQGRQAHGGLGLGLAIVRHLVELHGGTVE
                     ARSDGEGQGATFVVSLPRAGSPAPSAVAAS"
BASE COUNT          321 a          835 c          795 g          310 t
ORIGIN      
        1 gcggcccacg atgtgggacg tcgacttccg caccgccgtg gcccaggccg agctggagga
       61 ccgcgaggtc cggggcgcgc tgcaccgcct gcgcttcggc atccacgacg acggaccgct
      121 gctgatcgcc acgacccggc ccgagttgct ggccgcctgc gtggccgtgc tggtccaccc
      181 cgaggacagc cgctaccggc gcctgagcgg cttccacgcg atcacgccgc tcttcggcgc
      241 gcgcgtgccc gtcctcgccg atgccaaggt ggatcccgag aagggcacgg gcgcggtcat
      301 ggtctgcacc ttcggcgatg ccaccgacgt cgagtggtgg cgcgagtacg agctgccgct
      361 ccgacaagtg atcgacccgg cggggcggct cgcgccggtg acgttcgggt cgccgggctg
      421 ggagtcggcg gacccggacc gcgccaatcg ggcctaccgc cagctcgccg gcctcgacgt
      481 cggcaaggcc cgcacgcgcg tcgtcgagct gctgcgcgag gcgggggcgc tggatggcga
      541 gcccgagccg ctcacgcata cggtgaagtt cttcgagaaa ggggaccgac cgctcgagct
      601 gatccctgcc cgccagtggt tcgtccggct cctcgaccac cgggccgagc tgatcgagca
      661 aggcgagcgc atcgagtggc atcccgcctt catgcgcgcg cgttaccgca actgggtgga
      721 tggcctgaac caggactggt gcatcagccg gcagcgctat ttcggggtac ccttcccggt
      781 ctggtacccg ctcgacagcc agggccagcc gcagtacgac cggccgatcc tggcgccggc
      841 agccatgctg cccgtcgatc cgctggtcca ggcgccgccc ggccacgacg agtcgcagcg
      901 gggcacgccg ggcggcttca tcggcgaccc cgacgtgcag gacacgtggg cgacgagctc
      961 gctgagcccc cagatccaca gtcgctggga gctggacccc gaacgccacc ggtccctttt
     1021 tcccatggac gtgcggccgc agtcgcacga gatcatccgc acgtgggcct tctacacgat
     1081 cgccaaggcc tggctgcacc atcgggacgt gccgtggcat cacgcggtga tcagcggctg
     1141 ggtgctcgat cccgaccgca agaagatgag caagagcaag ggcaacgtga tcacgcccct
     1201 gcacctgctc gacgcgtact cggccgacgg cgtccgctac tgggcggcgc gggcccggcc
     1261 cggggcggac accgcctacg acgagagcgt cttcaagatc ggcaaacgcc tggtgaccaa
     1321 gctctcgaac gccgggcgct tcgtgctcgg ctgcctgggc gaggtcgacg accgtcgcct
     1381 cgccccggac gcgatcacga cggagctcga ccgggccttc gcctcccggc tgggcgaggt
     1441 cgtgagccgg gccaccgagg cctacgagga cttcgactgg gcggccgcgc tggagggcgt
     1501 ggagggcttc ttctgggcgg agctgtgcga cgactatctg gagatcgtca agacgcgggc
     1561 ttaccgggag gcgctcgacg ccgggcgcct gagcgccctg gccaccctca ggctggcgct
     1621 gtccgtcgtg ctccgtctct tcgcccccgt gctaccgacc atcacggagg agctgtggag
     1681 ccggcgcttc gccacccccg acggccgcac ccggtcgatc cacacgagcc cctggcccac
     1741 cgacaccgag ctcgcctcga tcacggcgcc cgaacacgcc ggcgcgttcg aggccgcccg
     1801 ggccatcgtc ggcgccgtgc gccgggtcaa agggagcgcc aaggtcagcc tgcgctggcc
     1861 cgtcgcctcg atcagagcga ccgggggatc ggccgagatc gcggccgccc ggtcggtgct
     1921 cgacgacatc cgcgccgccg gcgtcatccg ggagatcgcc ttcgagcccg gtcaggacgg
     1981 ctgggccttc gacgttcgcc tggccgactc cgctcgcggg tgagtcgccg gccacctacg
     2041 aagcggccac ggcggacggc gccggcgatc cggcccgcgg caggctcacg acgaacgttg
     2101 cgccctgtcc ctcgccgtca ctgcgcgcct cgacggttcc gccgtgcagc tccacgaggt
     2161 ggcgcacgat cgcgaggccg aggccgagac caccgtgcgc ttgccggccc tgccggaacc
     2221 gctcgaagac gtgggggagc aggtcggcac tgatcccctg a
//
LOCUS       DATIAZ010000132         7685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_295797, whole genome shotgun sequence.
ACCESSION   DATIAZ010000132 DATIAZ010000000
VERSION     DATIAZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295797"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VLF19_04615"
     CDS             <1..148
                     /locus_tag="VLF19_04615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913078.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSE92566.1"
                     /translation="GPKPYRMKWRGPSFSNLAILPHIIPGHKVADVVAIMGSVDPVFG
                     EVDR"
     gene            145..1140
                     /gene="nuoH"
                     /locus_tag="VLF19_04620"
     CDS             145..1140
                     /gene="nuoH"
                     /locus_tag="VLF19_04620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSE92567.1"
                     /translation="MTLPPVAQDALAAFVLLNAIILAVTYITLLERKFAARMQSRIGP
                     YYVGWPHGWLQPIADALKLMLKEDVVPTPADRGVYNLAPLVFLVPCMLIFATIPFAPG
                     LGLADLNLGVLFFLAVSALEIVGLFMGGWGSNNKYALLSAMRAVNQIISYDLPFIFAA
                     LVPVALAGSLQMSAVVGAQAGLGGWFVFYPVIGQLAFVAFLVATIAAENRVPFDILEA
                     ESELVAGFRVEYSGMKFALIQLGEYAHMLGTSFLGALLFLGGWLGPGPAWLAPLWFLL
                     KAVFVFLIVTWVRWSFVRIRVDQILAISWKLLLPATVVLLLVTGFIVAAGGGRGA"
     gene            1130..1693
                     /locus_tag="VLF19_04625"
     CDS             1130..1693
                     /locus_tag="VLF19_04625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HSE92568.1"
                     /translation="MARNGTAPAGFWSELGQLTRAVGRAMGVTLRNLFRRPVTVMYPD
                     APRPYPDRFRGVLALTYDRETGEENCIGCRLCEYICPPQVIKVEMLKAEKRNFARTFT
                     LELYACEFCELCVQVCPTDAIIMLKTFDLATTDRREMLLDKDRLHALGLDFQPSWATG
                     NRLRDMQAPPKPARGEAKAEAKGDGKE"
     gene            1720..2187
                     /locus_tag="VLF19_04630"
     CDS             1720..2187
                     /locus_tag="VLF19_04630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSE92569.1"
                     /translation="MAAALLASSLAVVLTPNLFHAVLYLAAALVATSAVFLLLDSPFL
                     FAVQLLLYAGGVVTVMVFAIMLTERLVGERITQTNRWVLNGAVVSAAVLVGVLGFVIR
                     TGGSAVAPPPAAVDRTADVARAVVGPFALPFELLGVLLVAALLGALYFARTED"
     gene            2190..2498
                     /gene="nuoK"
                     /locus_tag="VLF19_04635"
     CDS             2190..2498
                     /gene="nuoK"
                     /locus_tag="VLF19_04635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSE92570.1"
                     /translation="MGAPGLHAYLVLAALVFVIGLFGVLTRRNAIGILLGIELMLNAV
                     NINLVAFARFHGDLAGVVFTLFIISITVAEVGVGLAIVIAIFRGRKTIEADHLNLLRG
                     "
     gene            2502..4352
                     /gene="nuoL"
                     /locus_tag="VLF19_04640"
     CDS             2502..4352
                     /gene="nuoL"
                     /locus_tag="VLF19_04640"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSE92571.1"
                     /translation="MPPTIAPALIVLGLPAAAFTLLALAAPLRRRGRTAGLVSIAAVA
                     GAFLTALGVWRASVGADRGGVATWAWIPADGGPVASVGVLVDELSAAMLVLVTLVSLL
                     VQVYSLAYLSDEPAPGLGRYYTYQSLFAFSMLGLVLAPNFLQMFVFWELVGLCSYLLI
                     GYWYDRPAAARAAVKAFWVTRLGDVGFIIGIVMLWAATGTFEFQTLFRMAADRTLPLA
                     SLATITTLIWLGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVTAGVFLVTRAMP
                     LFALTPDVLALVGWVGAFTALLAATMACVEGDIKRVLAYSTVSQLGYMMAAAGAGAEN
                     AGFFHLLTHGVFKALLFLGAGAVIHAVGTNDVFRMGGLFRALPQTGVVFLVGTLALAG
                     VWPLAGFFSKEAILAGVWEARMTVPFLMLALTAFLTAFYMFRVVFIAFFGRVHAPHGA
                     RGHLHDAPPLMAGPLWVLAALTVALGVRFAIAGAGGEHHGPGWLAPLSLGLAAAGVAL
                     AWAMYQRGAVDPARLAAALGPLDHAARHRYGLDALYTGLYRAFILGFSRLIGWIDRYL
                     VDGVLNVLSSWTLRAGDRLRWIQSGRPQDYVYGVAFGVLLLLVWVQFFGR"
     gene            4349..5878
                     /locus_tag="VLF19_04645"
     CDS             4349..5878
                     /locus_tag="VLF19_04645"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSE92572.1"
                     /translation="MTIFPLLSLITWAPFVGALLIMFTARHSPRAVRLIAAVATGVST
                     VASVWIYAAYDREAAGFQFYERLPLVPPLGITYELGVDGMSLLMVLLTSVIIFAGVFA
                     SWTVTVRSQEFYALLLALVTGVYGVFVSLDLFVFFLFYELAVLPMYLLIGIWGSTGEV
                     RPRGIFAWAYRETGVGTKEYAAMKLTLYLLFGSAFILVGIFALYVNAGITSFSFLELE
                     AVAFAPDVQRWVFFAFYVGFGILAGIWPLHTWSPDGHASAPTAVSMLHAGVLMKLGAY
                     GVVRLGMGLLPAGAADLVWLVGTIACINIVYGALSAMAQVDLKYVIAYSSVSHMGIVM
                     LGAATLTEVGLNGSVFQMFAHGVMTGLFFALVGLVYEKAHSREIFKMGGFGRVMPGIA
                     VAFTVGGLSSLGLPATAGFVAEVLTFMGAWRSAHAWWLFPAVAGTFLTAIYVLRVTRQ
                     IFWGPPSPHFRHLEDARGPEWVALVILVAVLVAFGVAPGLAIGPVDTATVPLLARILL
                     P"
     gene            5875..7284
                     /locus_tag="VLF19_04650"
     CDS             5875..7284
                     /locus_tag="VLF19_04650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSE92573.1"
                     /translation="MSAFTLEIGTAGLLLVVFLATLAARGEDRRWIGWLATAGTLALT
                     GLALASAPAPPQLGGAFVHDGFALFAKRLFLGATFIGFLGGLQLPGPTFARRAGEYHV
                     LMLASLLGMLVLASSRDLVLLFVAFELMSIPLYVLAGFTKRETTSVEAALKFFLVGSV
                     SSAVMAYGLSFVYGATRTTSLGGVARVLPAGDPLLMVGLVAAFAGFGFKIAAVPFHMW
                     VPDAYEAAPTPFVAWLSVTPKAAGFVALVRVYFEGVGPEVARWVPLVAGLATLTIVAG
                     NLMALPQQNAKRLLAYSGIAHIGYMLVGLAAASPSGTAMVLFYLVAYVFGNMGAFLVV
                     DAVARAEGSEHVAAFRGLAQRSPLLALAMLLFLLSLGGIPFVAGFWAKLYVFWAAAAA
                     GLYWLVLVGAILTVVALFYYLVVARRMYIDAPAASGRVPVPVTLAAALILCLLGVVGL
                     GVWPKPVVMAALRVAAPLF"
BASE COUNT          821 a         2754 c         2670 g         1440 t
ORIGIN      
        1 cgggcccaag ccctaccgca tgaagtggcg cgggccctcg ttctccaacc tcgcgatcct
       61 gccccacatc atccccggcc acaaggtggc cgacgtcgtc gccatcatgg gctcggtcga
      121 cccggtcttc ggcgaggtgg accggtgacg ctgccgccgg tggcgcagga cgcgctggcg
      181 gcgttcgtcc tcctgaacgc gatcatcctc gcggtgacct acatcacgct gctcgagcgc
      241 aagttcgccg cgcgcatgca gtcgcgcatc ggcccatatt atgtcgggtg gccccatggc
      301 tggctgcagc ccatcgccga cgccctgaag ctgatgctga aggaggacgt ggtccccacg
      361 ccggccgacc gcggggtcta caacctcgcg ccgctcgtct tcctcgtgcc gtgcatgctg
      421 atcttcgcca cgatcccgtt cgcgccgggc ctgggcctgg ccgacctcaa cctcggggtg
      481 ctcttcttcc tggccgtctc cgcgctcgag atcgtcgggc tcttcatggg cggctggggc
      541 tcgaacaaca agtacgcgct gctcagcgca atgcgggcgg tgaaccagat catctcgtac
      601 gacctgccgt tcatcttcgc ggcgctggtg ccggtcgcgc tcgccggctc gctgcagatg
      661 tccgccgtcg tcggcgcgca ggcgggtctc ggcggctggt tcgtgttcta tccggtgatc
      721 ggccagctcg ccttcgtcgc ctttctcgtg gccaccatcg ccgccgagaa ccgcgtgccc
      781 ttcgacatcc tcgaggcgga gtccgagctg gtggccggct tccgcgtcga gtacagcggc
      841 atgaagttcg ccttgatcca gctcggcgag tacgcgcaca tgctcgggac ctcgttcctg
      901 ggggcgctgc tcttcctggg cggatggctc ggtccggggc cggcgtggct ggcaccgctg
      961 tggttcctgc tcaaggcggt cttcgtgttc ctcatcgtca cgtgggtgcg ctggtcgttc
     1021 gtgcgcatcc gcgtggacca gatcctggcc atctcgtgga agctcctgct gccggcgacg
     1081 gtggtgctgt tgctggtcac cggcttcatc gtggcagcgg gaggcggccg tggcgcgtaa
     1141 cgggaccgcc cccgccggct tctggagcga gctcggccag ctgacgcgcg ccgtcggccg
     1201 cgccatgggc gtcacgctgc gcaacctgtt ccgcaggccc gtcaccgtca tgtaccccga
     1261 cgcgccgcgg ccgtacccag accgcttccg cggcgtgctg gccctgacct acgatcgcga
     1321 gaccggcgag gagaactgca tcggctgccg gctgtgcgag tacatctgcc cgccgcaggt
     1381 catcaaggtc gagatgctga aggccgagaa gcggaacttc gccaggacct tcacgctgga
     1441 gctgtacgcg tgcgagttct gcgagctctg cgtgcaggtc tgcccgaccg acgcgatcat
     1501 catgctcaag accttcgatc tcgccaccac ggaccgccgg gagatgctgc tcgacaagga
     1561 ccgcctgcac gcgctgggac tcgacttcca gccgtcgtgg gcgacgggca accggctgcg
     1621 cgacatgcag gcgccgccca agccggccag gggcgaggcg aaggccgagg cgaaggggga
     1681 cgggaaggag tgacgggcga ggggatagcc ttctggctgg tggccgccgc gctgctggcc
     1741 tcgtcgctgg ccgtcgtgct cacgcccaac ctgttccacg ccgtgctgta cctggcggcg
     1801 gcactggtcg ccaccagcgc cgtgttcctg ctgctggact cgccgttcct cttcgccgtc
     1861 cagctcctgc tctacgcggg cggtgtcgtg accgtgatgg tcttcgccat catgctgacc
     1921 gaacggctcg tgggcgagcg catcacgcag acgaaccggt gggtgctgaa cggggccgtg
     1981 gtctcggcgg ccgtgctggt gggcgtcctc ggtttcgtga tccgcaccgg ggggtcggcc
     2041 gtggcgccgc cgccggccgc cgtcgaccgt accgccgacg tcgcccgcgc cgtcgtcggg
     2101 ccgttcgcgc tgccgttcga gctgctgggg gtcctcctgg tggccgctct cctcggcgcg
     2161 ctctacttcg cgcggacgga ggactgagcg tgggcgcgcc cggactccac gcctacctgg
     2221 tcctcgctgc gctcgtgttc gtcatcgggc tgttcggcgt gctcacccgc cgcaacgcga
     2281 tcggcatcct cctcggcatc gagctgatgc tgaacgccgt caacatcaac ctggtcgcgt
     2341 tcgcccgctt ccacggcgac ctggccggcg tggtcttcac cctgttcatc atctccatca
     2401 cggtggccga ggtcggcgtg ggtctggcga tcgtcatcgc gatcttccgc ggccgcaaga
     2461 ccatcgaggc ggaccacctg aacctcctgc gaggctgacg cgtgcccccg accatcgccc
     2521 cggcgctgat cgtcctcggc ctgcccgcgg ccgccttcac gctgctcgcg ctggcggcgc
     2581 cgctccggcg tcgggggcgc acggccggtc tcgtgagcat cgccgccgtg gccggggcgt
     2641 tcctgaccgc gctcggcgtg tggcgcgcca gcgtcggcgc cgaccgcggc ggagtggcca
     2701 cgtgggcctg gatccccgcc gacggcggcc cggtcgcgag cgtcggcgtc ctggtcgacg
     2761 agctgagcgc ggccatgctc gtgctggtca cgctggtctc gctgctcgtg caggtgtact
     2821 cgctcgcgta tctgtcggac gagccggccc ccggtctcgg gcgctactac acctaccagt
     2881 cgctgttcgc cttctcgatg ctcgggctcg tgctggcgcc gaacttcctc cagatgttcg
     2941 tgttctggga gctggtcggc ctctgctcgt atctgttgat cgggtactgg tacgaccggc
     3001 cggcggcggc gcgcgccgcg gtgaaggcgt tctgggtcac ccgcctggga gacgtgggct
     3061 tcatcatcgg catcgtcatg ctgtgggcgg ccaccgggac cttcgagttc cagacgctgt
     3121 tccgcatggc cgccgatcgg acgctgccgc tggcgtcgct ggccacgatc acgacgctga
     3181 tctggctcgg cgccgtcggc aagtcggcgc agttcccgct gcacgtctgg ctccccgacg
     3241 cgatggaggg cccgactccg gtgtccgccc tcatccacgc ggcgaccatg gtgacggccg
     3301 gcgtgttcct cgtcacgcgc gcgatgccgc tgttcgcgct cacgcccgac gtgctggccc
     3361 tggtgggctg ggtcggcgcc ttcaccgccc tgctcgccgc gaccatggcc tgcgtcgagg
     3421 gcgacatcaa gcgcgtgctg gcctactcca cggtctcgca gctcggctac atgatggccg
     3481 ccgccggcgc cggcgccgag aacgcgggct tcttccacct gctgacgcac ggcgtgttca
     3541 aggcgctgct cttcctcggc gccggcgccg tcatccacgc cgtggggacc aacgacgtct
     3601 tccgcatggg cggcctgttc cgcgcgctgc ctcagacggg cgtggtcttc ctggtcggca
     3661 cgctggcgct ggccggcgtc tggccgctgg ccgggttctt ctcgaaagag gcgatcctgg
     3721 ccggcgtgtg ggaggcgagg atgaccgtgc ccttcctgat gctggcgctg acggcgttcc
     3781 tcaccgcctt ctacatgttc cgggtggtct tcatcgcctt cttcggccgc gtgcacgcgc
     3841 cgcacggggc gcgcggccac ctgcacgacg cgcccccgct catggccggt ccgctctggg
     3901 tgttggccgc gctgacggtg gcgctcggag tccggttcgc gatcgccggc gccggcgggg
     3961 agcaccacgg ccccggctgg ctggccccgc tctcgctcgg cctggccgcg gccggtgtcg
     4021 ccctggcgtg ggcgatgtac cagcgcggcg ccgtcgatcc cgcgcgcctg gcggccgcgc
     4081 tggggccgct cgaccatgcc gcgcggcatc ggtacggcct ggacgcgctc tacaccgggc
     4141 tctaccgggc cttcatcctg ggcttttccc gcctcatcgg ctggatcgac cgatacctgg
     4201 tggatggcgt cctgaacgtg ctgtcctcgt ggacccttcg cgccggcgac cggctgcggt
     4261 ggatccagag tggtcggcct caagactacg tctacggcgt cgcgttcggc gtgctgctcc
     4321 tcctcgtgtg ggtccagttc ttcggccgat gacgatcttc ccgctcctgt cactgatcac
     4381 gtgggcgccc ttcgtcggcg ccctgctcat catgttcacg gcgcggcact ccccgcgcgc
     4441 cgtgcgcctg atcgcggccg tcgccacggg cgtctcgacg gtggcctcgg tctggatcta
     4501 cgccgcctac gaccgggaag cggccggctt ccagttctac gagcggctgc cgctggtgcc
     4561 gccgctgggc atcacgtacg agctgggcgt ggacggcatg agcctgctca tggtgctgct
     4621 cacatcggtc atcatcttcg ccggcgtctt cgcctcgtgg acggtgaccg tgcgcagcca
     4681 ggagttctac gcgctgctgc tggccctggt gaccggcgtc tacggcgtgt tcgtctcgct
     4741 ggacctgttc gtcttcttcc tcttctacga gctggccgtg ttgccgatgt acctgctcat
     4801 cggcatctgg ggctcgacgg gcgaggtgcg cccgcggggc atcttcgcgt gggcctatcg
     4861 cgagacgggc gtgggcacca aggagtacgc ggccatgaag ctgaccctgt acctgctctt
     4921 cgggtcggcg ttcatcctcg tggggatctt cgccctgtac gtcaacgccg ggatcacgtc
     4981 gttctcgttc ctcgagctgg aggccgtcgc cttcgccccc gacgtccagc gctgggtgtt
     5041 cttcgccttc tacgtgggtt tcggcatcct ggccggcatc tggcccctgc acacctggtc
     5101 ccccgacggc cacgcctcgg cgccgaccgc ggtgtccatg ctccacgccg gcgtgctgat
     5161 gaagctcggc gcctacgggg tcgtgcgcct gggcatgggc ctcctgcccg cgggcgccgc
     5221 cgacctcgtg tggctggtag ggacgatcgc ctgcatcaac atcgtgtacg gggcgctgtc
     5281 ggcgatggcc caggtggacc tcaagtacgt catcgcctat tcgtcggtct cgcacatggg
     5341 catcgtcatg ctgggcgcgg ccacgctcac cgaggtcggg ctcaacggct ccgtcttcca
     5401 gatgttcgcc cacggcgtga tgacggggct cttcttcgcc ctcgtcgggc tcgtctacga
     5461 gaaggcgcac tcgcgggaga tcttcaagat gggtgggttc gggcgggtca tgcccggcat
     5521 cgccgtggcc ttcaccgtcg gcggcctgtc gtcgctgggc ctgcccgcga ccgccggctt
     5581 cgtcgccgag gtgctcacgt tcatgggcgc gtggcgctcg gcgcacgcgt ggtggctctt
     5641 cccggcggtg gccggcacct tcctcaccgc gatctacgtg ctgcgcgtga ccaggcagat
     5701 cttctggggg ccgccgtcgc cccacttccg ccacctcgag gacgcgcgcg ggcccgagtg
     5761 ggtggcgctc gtcatcctcg tcgccgtgct ggtggcgttc ggcgtggccc ccggcctcgc
     5821 catcggcccg gtggacaccg cgacggtgcc gctcctggcc cggatcctcc tgccgtgagc
     5881 gccttcacgc tcgagatcgg caccgcgggc ctgctgctgg tggtcttcct cgccaccctg
     5941 gcggcgcgcg gcgaggaccg gcggtggatc gggtggctcg cgaccgccgg cacgctggcg
     6001 ctcaccgggc tggccctggc gtcggcgccg gctccgccgc agctcggcgg cgcgttcgtg
     6061 cacgacggct tcgcactctt cgcgaagcgc ctgttcctcg gcgccacgtt catcggattc
     6121 ctcggcgggc tccagctgcc cggcccgacc ttcgcgcggc gcgcgggcga gtaccacgtg
     6181 ctcatgctgg cctcgctgct gggtatgctc gtccttgcgt cgtcgcggga cctggtgctg
     6241 ctcttcgtcg ccttcgagct gatgtcgatc ccgctctacg tcctcgccgg gttcaccaag
     6301 cgcgagacga cgtcggtcga ggctgcgctc aagttcttcc tggtcggcag cgtgtcatcg
     6361 gccgtgatgg cgtacgggct gtcgttcgtc tacggggcga cgcggacgac gagcctcggt
     6421 ggcgtggcgc gcgtgttgcc ggccggggac cccctgctga tggtgggcct cgtggctgcc
     6481 ttcgcggggt tcggcttcaa gatcgccgcc gtcccattcc acatgtgggt accggacgcc
     6541 tacgaggcgg cgccgacgcc cttcgtcgcc tggctcagcg tgacgccgaa ggccgcgggc
     6601 ttcgtcgcgc tggtgcgcgt gtacttcgag ggcgtggggc ccgaggttgc gcgctgggtg
     6661 ccgctggtgg cggggctggc gacgcttacc atcgtcgccg gcaacctcat ggcgctgccg
     6721 cagcagaacg ccaagcgcct gctcgcgtac tccggcatcg cgcacatcgg ctacatgctg
     6781 gtcgggctcg ccgccgcctc gccctcgggc acggcgatgg tgctcttcta cctggtggcc
     6841 tacgtcttcg gaaacatggg cgccttcctg gtcgtcgacg ccgtggcccg tgcggagggc
     6901 tcggagcacg tggccgcgtt ccggggcctc gcgcagcgct cgccgctgct cgcgctggcc
     6961 atgctgctct tcctgctctc gctcggcggc atccccttcg tcgccgggtt ctgggccaag
     7021 ctgtacgtgt tctgggcggc ggccgccgcc ggcctctact ggctcgtcct ggtcggcgcg
     7081 atcctgaccg tggtggcgct cttctactat ctggtcgtcg ccaggcggat gtacatcgac
     7141 gcgccggccg cgtccgggcg cgtgcccgtg ccggtgacgc tcgccgccgc gctgattctc
     7201 tgcctcctcg gcgtcgtcgg gctgggcgtg tggccgaagc cggtggtgat ggccgcgctg
     7261 cgggtggccg cgccattgtt ctagtcgggg ggagcggcgc ccctcccccg ctcccccacc
     7321 gggatcgcgc gagctgggga acagggattg cgcgggcgaa gcccgagtgc gggccgcgct
     7381 ttcaccagtg gctgtttgcc ccccgtaggc cggggcggga tcggggtcgg agcgtgggtc
     7441 cgccgacctg tttcccgcca acggaacgtc gcccgacgcg acgatggaaa tagcactcgc
     7501 cggacagggc gctgcgcatc aggggtcggc ggacccacgc tccgaccccg atcccgcccc
     7561 ggccggagca tcaggtcacg gcggttggac tctccagcgc tattgcctgg cggtgtcctc
     7621 gacgacctcg tagacgacgc ggcgggagcg catccaccgg acggcgagga ggtcggcgaa
     7681 agcgc
//
LOCUS       DATIAZ010000133         5006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1348132, whole genome shotgun sequence.
ACCESSION   DATIAZ010000133 DATIAZ010000000
VERSION     DATIAZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348132"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VLF19_04655"
     CDS             complement(<1..305)
                     /locus_tag="VLF19_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSE92574.1"
                     /translation="MTGGPRAAAWAVVPSPRRVRAALGPPGALALLVLAVVAPSPAAE
                     LWQWMDAEGVVRYTNDPETIPPAFRGGARDVGSPQPRPSTPSAPAGTPPALPVPAGTA
                     "
     gene            complement(302..1648)
                     /locus_tag="VLF19_04660"
     CDS             complement(302..1648)
                     /locus_tag="VLF19_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92575.1"
                     /translation="MVELERRGIPTVIFTARHFLHDAERTAASLGLPGLPLALVPLPF
                     TNQTAEAVHRMVDAAIEQVIAGLTKPVAEPGPVRHPAPDERLVYDGEDAYAAWEAMNR
                     DFLTRGWSDGFPLVAPTERAVAAMLRGTQRAPGDQVVVLEPGFGIATVQKLAVNAVMA
                     GCAPAHLPLLIAAVRCLAEPKMYIRNKAMSTGPHAPLVLVNGPKGRAAGLHGGMCALG
                     PGAPSVANTVLGRAVRLAMMNVGHTYVGSSDMDTIGSPLKYSLCCAENERESPWEPYH
                     VTRGFAKDASAVTVHFVYGMCELHDFQSTTPEALVEVFATAATNVAQVGTGLWLIGRR
                     ADPRYKTEEKEHNMLFVCPEHARVFAGAGWGRTRIQEAMYRAARVPFRTLALNKERKA
                     MAVAHPELGWLAEHPDLPLPVVEEPACYDVAVVGAAAGRGAYFYGAGEPVTMPVDE"
     gene            complement(1679..1972)
                     /locus_tag="VLF19_04665"
     CDS             complement(1679..1972)
                     /locus_tag="VLF19_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92576.1"
                     /translation="MIRLEILSPLAKVVEFSVKPAARLADWQGVTIGLYWNIKAGGDF
                     ALDRTEELLGQKFPGVKFKRYTGSVGWLMRHCTAEDADRIASEVHAVVGTTND"
     gene            complement(1988..3187)
                     /locus_tag="VLF19_04670"
     CDS             complement(1988..3187)
                     /locus_tag="VLF19_04670"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92577.1"
                     /translation="MTQPALALDGVRVLDLSRVLAGPFCGMLLADLGADVVKIEDTAA
                     GDESRTWPPHKDGESAAYLVINRNKRDMTLDLKHPEGAEILRRMVQDADVLIENFRTG
                     TMESFGLGYDALAAINPRLVYCSISAFGRTGPRAEGAGYEALMQAFSGIMSITGEPGG
                     PPVRCGVSFLDLTTGIVCAFGIVNALLHRERTGLGQRVDGSLLDTAVSLLNYHAEGYL
                     LTGAVPRALGSSHPSLSPYRNFRCRDGQWVFIAGANDRFWARLAPALGLPEMANDPKF
                     ATNIERVKHRRELEAALEEAIARHDRAPLLKTLEEAGVPATPVNTVDQVMDDPQTTAR
                     GIIERVLHPTLGEIPVVATPVKFSRMRPGVRTAAPLRGQHTDEVLAAYGYSAAAIAAL
                     RTKKVVV"
     gene            3207..>5006
                     /locus_tag="VLF19_04675"
     CDS             3207..>5006
                     /locus_tag="VLF19_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HSE92578.1"
                     /translation="MSRLREALERRILVLDGAMGTMLQARALTAADFGGAQYEGCNEH
                     LNLTRPDVVRAIHRAYAEAGADIVSTNTFGCAPWVLGEYGLGERAYEITRTAARLARE
                     AAGDAFVAGSMGPGTRSISVTRNITFAEVREGYALQARALIDGGVDALLLETQQDTLN
                     LKASAIGIGRAIAESGRAVPLMVSITIEPTGTMLAGQGVEALYVALQHLAVLSLGLNC
                     STGPEFMTDHLRTLAQMATCFVSVYPNAGLPDERGQYGETAESLAFKMRRFVDEGWVN
                     VVGGCCGTTAEHVRALARLCAGRPPRVPARAEPQAVSGVEVLYPTEDNRAVFVGERTN
                     VIGSRRFKELIVEERFEEAAEIGRAQVRGGAQVLDVCLANPDRDEARDMDRFMASLTR
                     MVKAPLMIDSTDAAVIELALRHCQGKAIVNSINLEDGEERFERVVPLLRTYGAAVVVG
                     CIDEDKQQGMAVTAGRKLAIAERSHALLTGTYGLPARDLIFDPLVFPVGTGDRNYVGA
                     AVETIEGVRLIKARFPECKTILGISNVSFGLPSAGREVLNAVFLYHCTKAGLDYAIVN
                     TERLERYASIPEDERRLAEDLIFQRGADPVAAFA"
BASE COUNT          717 a         1784 c         1790 g          715 t
ORIGIN      
        1 gcggtgccgg ccggcaccgg gagtgccggc ggcgtgcccg cgggcgccga cggcgtcgag
       61 gggcgaggct gcgggctacc gacgtcgcga gcgcccccgc ggaaggccgg tgggatcgtc
      121 tcggggtcgt tcgtgtagcg cacgacgccc tccgcgtcca tccactgcca cagctccgcc
      181 gccggcgacg gcgccaccac cgccagaacg agcagggcga gagcacccgg cgggccgaga
      241 gcggcccgta cgcgtcgcgg cgacggcacg acggcccagg cggccgctct cggcccgccg
      301 gtcactcgtc caccggcatc gtgacaggct cgccggcgcc gtagaagtag gcgcctcgcc
      361 cggccgcggc gccgaccacc gcgacgtcgt agcacgccgg ctcctcgacc acgggcaacg
      421 gcagatccgg gtgctcggcg agccagccga gctccgggtg cgcgacggcc atcgccttgc
      481 gctccttgtt gagtgccagc gtgcggaagg gcacgcgggc ggcgcggtac atcgcctcct
      541 ggatgcgggt gcgcccccag cccgcccccg cgaagacccg agcgtgctcg gggcagacaa
      601 agagcatgtt gtgctccttc tcctcggtct tgtagcgcgg gtcggcgcgc cggccgatca
      661 gccagagtcc cgtgcccacc tgggcgacgt tcgtcgccgc ggtggcgaaa acctcgacga
      721 gtgcctccgg cgtcgtggac tggaagtcgt gcagctcgca catgccgtag acgaagtgaa
      781 cggtgaccgc gctcgcgtcc ttggcaaagc cccgcgtgac gtggtacggc tcccacgggc
      841 tctcgcgctc gttctccgcg cagcagagac tgtacttgag cggcgagccg atggtgtcca
      901 tgtcgctcga gcccacgtag gtgtgcccca cgttcatcat cgcgagcctg acggcgcggc
      961 cgagcacggt gttggccacc gagggcgctc ccgggcccag ggcacacatc ccgccgtgca
     1021 gcccggccgc ccggcccttc gggccgttga cgaggacgag cggtgcgtgc gggcccgtgg
     1081 acatggcctt gttgcgaatg tacatcttcg gctccgccag gcagcgcacg gcggcgatca
     1141 gcagcggcag gtgcgccggg gcgcagccgg ccatgaccgc gttgacggca agcttctgga
     1201 cggtcgcgat gccgaagccg ggctccagga cgacgacctg gtcgccgggc gcccgttggg
     1261 tcccccgcag catcgccgcg accgcgcgtt ccgtcggcgc caccagcggg aagccgtcgg
     1321 accagcctcg cgtcaggaag tcgcggttca tcgcctccca ggcggcatag gcgtcctcac
     1381 cgtcgtagac gagccgctcg tccggggccg ggtgccggac gggcccaggc tcggccacgg
     1441 gcttcgtgag gccagcgatc acctgctcga tcgcggcgtc gaccatgcgg tggacggcct
     1501 ccgccgtctg gttcgtgaag ggcagcggca ccagcgccag cggcagcccg ggcagcccga
     1561 ggctcgccgc cgtgcgctcg gcgtcgtgca ggaagtgccg cgccgtgaag atgacggtcg
     1621 ggatcccccg acgctcgagc tcgaccatgt cgtggacacc ccacgacgtg cacgaccctc
     1681 agtcgttggt ggtgccgacg acggcgtgca cctccgacgc gatgcggtcg gcgtcctcgg
     1741 ccgtgcagtg acgcatgagc cagccgaccg acccggtata gcgcttgaac ttcacgccgg
     1801 ggaacttctg gccgaggagc tcctcggtcc ggtcgagcgc gaagtcgccg ccggccttga
     1861 tgttccagta cagcccgatc gtcacgccct gccagtcggc gaggcgcgcg gccggcttga
     1921 cggaaaactc gaccaccttc gccagcggac tgaggatctc cagccgtatc atcggttcct
     1981 cccggagcta gacgacgacc ttcttggtgc gcagcgcggc gatcgcggct gccgagtagc
     2041 cgtacgccgc cagcacctcg tcggtgtgct ggcctctcag cggcgccgcc gtccggacgc
     2101 cgggacgcat gcgcgagaac ttcacgggcg tggccacgac ggggatctcg ccgagcgtgg
     2161 ggtgcaggac ccgctcgatg atgccgcgcg ccgtcgtctg cgggtcgtcc atcacctggt
     2221 ccacggtgtt gacgggcgtc gccggcacgc cggcctcctc gagcgtcttc agcagcggcg
     2281 cgcggtcgtg gcgggcgatc gcctcctcga gcgccgcttc cagctcgcgc cggtgcttca
     2341 cgcgctcgat gttggtcgcg aacttcggat cgttggccat ctccggcagg ccgagggcgg
     2401 gcgcgagccg cgcccagaag cggtcgttcg cgccggcgat gaagacccac tggccgtccc
     2461 ggcagcggaa gttccggtag ggggagagcg acgggtgcga cgagccgagc gccctgggca
     2521 ccgcgccggt gagcagatag ccctcggcgt gatagttcag caggctcacc gccgtgtcga
     2581 gcagcgagcc gtccacgcgc tggccgagcc ccgtgcgctc gcggtggagg agcgcgttga
     2641 cgatgccgaa ggcacagacg atgccggtcg tgagatcgag gaacgacacg ccgcaccgca
     2701 ccgggggtcc gccgggctcg ccggtgatcg acatgatccc gctgaaggct tgcatgaggg
     2761 cctcgtagcc ggcgccctcg gcccgcggcc ccgtgcgccc gaacgccgag atcgagcagt
     2821 agacgagccg ggggttgatc gccgccagcg catcgtagcc gagcccgaac gactccatcg
     2881 tgcccgtccg gaagttctcg atgaggacgt cggcgtcctg caccatgcgg cggaggatct
     2941 cggcaccctc cgggtgcttg agatcgagcg tcatgtcacg cttgttgcgg ttgatgacga
     3001 ggtaggccgc cgactcgccg tccttgtggg gcggccacgt ccgggactcg tcgccggcgg
     3061 ccgtgtcctc gatcttgacg acgtcggcgc cgaggtcggc aaggagcata ccgcagaagg
     3121 gaccggccag gacacgcgag agatcgagta cgcggacgcc gtcgagggcg agggcaggct
     3181 gggtcatgtg cgctactata atctccatga gccggctgcg cgaggcgctc gaacgtcgca
     3241 tcctcgtcct ggacggggcg atgggcacga tgctccaggc ccgcgcgctg acggcggcgg
     3301 acttcggcgg cgcccagtac gaggggtgta acgagcacct gaacctcacg cgtcccgacg
     3361 tcgtgcgcgc catccaccgc gcgtacgcgg aggccggcgc cgacatcgtg tccaccaaca
     3421 ccttcggctg cgcgccgtgg gtgctgggcg agtacgggct cggcgagcgc gcgtacgaga
     3481 tcacgcggac ggcggcgcgg ctggcgcgcg aggccgccgg cgacgccttc gtcgcgggct
     3541 ccatgggccc cggcacgcgc tcgatctccg tcacgcgcaa catcacgttc gccgaggtgc
     3601 gcgagggtta cgcgctgcag gcccgcgcgc tgatcgacgg cggcgtggac gcgttgctgc
     3661 tcgagaccca acaggacacc ctcaacctga aggcgtcggc catcggcatc ggccgggcca
     3721 tcgccgagag cggccgcgcc gtacctctca tggtgagcat caccatcgag cccaccggca
     3781 cgatgctcgc cggccagggc gtggaggcgc tgtacgtcgc gctccagcac ctcgccgtcc
     3841 tctcgctcgg gctcaactgc tccaccggcc cggagttcat gaccgatcac ctacgcacgc
     3901 tggcgcagat ggccacctgc ttcgtcagcg tgtacccgaa cgccgggctg cccgacgagc
     3961 gcggtcagta cggcgagacg gccgagagcc tcgccttcaa gatgcggcgg ttcgtggacg
     4021 agggctgggt gaacgtggtc ggcggctgct gcgggacgac ggccgagcac gtccgcgcgc
     4081 tggcgcgcct ctgcgccggc cgcccacccc gtgtgcccgc gcgcgccgag ccgcaggcgg
     4141 tgagcggcgt ggaggtcctc tacccgaccg aggacaaccg tgccgtcttc gtcggcgagc
     4201 gcacgaacgt catcggctcg cggcgcttca aggagctgat cgtcgaggaa cggttcgagg
     4261 aggcggcgga gatcggccga gcccaggtgc gcggcggggc tcaggtgctc gatgtgtgcc
     4321 tcgccaaccc cgaccgcgac gaggcccggg acatggatcg cttcatggcc tcgctcaccc
     4381 gcatggtgaa ggcaccgctg atgatcgact ccaccgatgc cgccgtcatc gagctggccc
     4441 tccggcactg tcagggcaag gccatcgtca actcgatcaa cctggaggac ggcgaggagc
     4501 gcttcgagcg cgtcgtcccc ctgctccgga cctacggggc ggcggtcgtc gtcggctgca
     4561 tcgacgagga caagcagcag ggcatggcgg tcaccgccgg ccggaagctc gccatcgccg
     4621 agcgctcgca cgcgctgctg accgggacgt acggtcttcc cgcccgcgac ctgatcttcg
     4681 acccgctggt ctttccggtc ggcaccggcg accggaacta cgtgggcgcg gcggtcgaga
     4741 ccatcgaggg tgtccggttg atcaaggcgc gcttccccga gtgcaagacc atcctcggga
     4801 tctccaacgt gtcgttcggc ctgccgtcag ccggccgcga ggtgctcaac gcggtcttcc
     4861 tctaccactg caccaaggcc ggcctggact acgcgatcgt caacaccgag cggctcgagc
     4921 gctacgcctc gatccccgag gacgagcggc gcctggccga ggatctgatc ttccagcgcg
     4981 gcgccgatcc ggtggcggcc ttcgcc
//
LOCUS       DATIAZ010000134         4182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_163937, whole genome shotgun sequence.
ACCESSION   DATIAZ010000134 DATIAZ010000000
VERSION     DATIAZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163937"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1127)
                     /locus_tag="VLF19_04680"
     CDS             complement(105..1127)
                     /locus_tag="VLF19_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92579.1"
                     /translation="MMLTPLTRRRLAGFRANRRGYWSLWVFLALFVLSLGADVLANDR
                     PLLAWYEGRIYVPVFRAYPETAFGGELPTEAAYRDPEVRRLIEARGWMLWPPIPYRYD
                     TVDWGLAGPAPAPPSTRHWLGTDDQARDVLARLVYGFRISVLFGLTLTLASSVIGVAA
                     GAVQGYFGGWTDLLFQRFIEVWSGLPVLYLLIILASIVQPNFWWLLGLMLLFSWMALV
                     GVVRAEFLRGRNFDYVRAARALGVRNAVIMFRHLLPNAMVATLTFLPFILNGSITTLT
                     SLDFLGFGLPPGSASLGEILAQGKANLQAPWIGLTGFVVLAVMLSLLIFIGEAVRDAI
                     DPRKVQ"
     gene            complement(1138..2244)
                     /locus_tag="VLF19_04685"
     CDS             complement(1138..2244)
                     /locus_tag="VLF19_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HSE92580.1"
                     /translation="MIAYLARRLLLIVPTLFGIMLANFLIVQAAPGGPVDLMIARLRG
                     HAGEATARVGGSGAAEVGGRAAQPTAGRGEEASRAARGVDPELIRQLEQQFGFDKPAW
                     QRFAQMMRNYLVFDLGTSFYRDRPVAGLMLEKLPVSISLGLWTTLLVYLVSIPLGVAK
                     AVRDGTPFDVWTSATVIVGYAIPSFLFAVLLVVVFAGGSFVQWFPLRGLVSDTWALLS
                     WPQRILDYFWHIALPVVALVAGGFAGLAMLTKNSFMDEIGKQYVVTARAKGLTRRRVL
                     YGHVFRNAMLIVVAGFPAAFVGILFTSALLIEVIFSLDGLGLLGFEAAVNRDYPVMFG
                     TLYVYTLIGLLLNIVTDLTYVAIDPRIDFERRDA"
     gene            complement(2241..4061)
                     /locus_tag="VLF19_04690"
     CDS             complement(2241..4061)
                     /locus_tag="VLF19_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE92581.1"
                     /translation="MRIAVAVALLLALAASAPGPRAAPAAGRPGHGLSMHGDLKYPPG
                     FAHFDYVNPRAPRGGKVTLAAIGTFDSLHPFILKGVAAAGIGAVFDSLTVQSADEPFS
                     EYGLVAETIETPPDRSWVAYTVRPQARFHDGSPVTVEDVIWTFETLRAKGHPFYRSYY
                     RQIASVEKAGPRRVLFTFKRGENRELPLIVGQMPVLPKAYWAGRDFAKTTLEAPLGSG
                     AYRVESFEPGRSITYRRVPDYWAAELPVSVGRFNFDVIRYDYYRDGIVALEAFKAGAY
                     DFRQENSAKSWARGYDVPAVRDGRIRKEQIRNEVPTGMQGFVYNTRRPVFGDPRVRQA
                     LARAFDFEWANAHLFYGAYTRTRSYFSNSELAARGLPGPDELAVLAPFRGRVPDEVFT
                     KEYRPPSTAPGGLRPGLVEALRTLKDAGWVVRDMRLVNAETERQMAFEILIEDANWER
                     ITLPFVKNLERLGIAARLRTVDAAQYEYRLKHFDFDVTVAVFAQSLSPGNEQADFWGS
                     EAADTPGSRNLAGIRDPVVDALVASLIAAPDRRSLVARTRALDRVLLWSHYVVPHWHI
                     TAFRVAYWNRFGRPAVSPKYELGFDTWWIDPRKTSPPGNG"
BASE COUNT          708 a         1439 c         1499 g          536 t
ORIGIN      
        1 cgcgtgcagc tcgccgccct ccacggcgaa cgacaccgtg agctcgcgca cggagaggag
       61 ggggcccacc gcgccgtccg cgtgctcccc ggcgttgccg gcgctcattg gaccttgcgg
      121 gggtcaatgg cgtcccgcac ggcctcgccg atgaagatga gcagcgagag catcacggcg
      181 agcacgacga agccggtgag cccgatccac ggcgcctgga ggttggcctt gccctgggcg
      241 aggatctcgc cgagcgaggc cgagcccggc ggcagcccga agccgaggaa gtcgagcgag
      301 gtcagcgtgg tgatcgagcc gttcaggatg aagggcagga acgtcagcgt ggcgaccatg
      361 gcgttcggca acaggtggcg gaacatgatg acggcgttcc tgacgccgag cgcgcgggcc
      421 gcccgcacgt agtcgaagtt gcgcccccgc aggaactccg cccgcaccac accgaccagc
      481 gccatccacg agaagagcag catgaggccg agcagccacc agaagttcgg ctgcacgatg
      541 ctggcgagga tgatcagcag atagaggacc ggcagccccg accacacctc gatgaaccgc
      601 tggaagagca ggtcggtcca gcccccgaag tagccctgca ccgcgccggc ggccacgccg
      661 atcacgctgc tcgccagggt cagcgtgagg ccgaagagca ccgagatccg gaagccgtag
      721 accagccgcg ccagcacgtc gcgcgcctgg tcgtcggtgc cgagccagtg gcgcgtcgac
      781 ggcggcgccg gcgccggccc cgcgagcccc cagtcgacgg tgtcgtagcg gtaggggatg
      841 ggcggccaga gcatccagcc gcgggcctcg atcagccgcc gcacctcggg gtcgcggtag
      901 gcggcctcgg tcggcagctc gccgccgaac gcggtctccg gatacgcgcg gaacaccggg
      961 acgtagatgc gcccctcgta ccacgcgagc aggggccggt cgttggcgag gacgtcggcg
     1021 ccgagcgaga gcacgaagag cgccaggaag acccacagcg accagtagcc ccgacggttc
     1081 gcccggaagc cggcgagtcg ccgacgtgtc agcggggtga gcatcacgta tcgtggctca
     1141 cgcgtcgcgt cgctcgaagt cgatgcgcgg atcgatggcc acgtaggtga ggtcggtgac
     1201 gatgttcagc aggagcccga tcagcgtgta gacgtagagc gtcccgaaca tcaccggata
     1261 gtcgcggttc acggcggcct cgaacccgag cagccctagt ccgtcgagcg agaagatgac
     1321 ctcgatcagc agcgcgctgg tgaagaggat gccgacaaag gcggcgggga agccggcgac
     1381 cacgatcagc atggcgttgc ggaagacgtg gccgtacagc acgcgccgcc gcgtgaggcc
     1441 cttggcccgg gccgtcacca cgtactgctt gccgatctcg tccatgaacg agttcttcgt
     1501 cagcatggcg aggccggcga agcccccggc gacgagggcg acgacgggca gggcgatgtg
     1561 ccagaagtag tcgaggatcc gctgcggcca cgagagcagc gcccacgtgt cggagacgag
     1621 tccccgcagc gggaaccact gcacgaacga gccgccggcg aagaccacca ccagcaggac
     1681 ggcgaagagg aagctgggga tcgcgtagcc cacgatgacc gtggcgctcg tccacacgtc
     1741 gaagggcgtg ccgtcgcgca cggccttggc cacgccgagc gggatcgaca ccagatagac
     1801 gagcagcgtc gtccacaggc cgagcgagat cgataccggc agcttctcga gcatgagccc
     1861 ggccaccggg cggtcgcggt agaaggacgt cccgaggtcg aagacgagat agttgcgcat
     1921 catctgcgcg aagcgctgcc aggccggctt gtcgaagccg aactgctgct cgagctggcg
     1981 gatcagctcg gggtccacgc cccgcgcggc acgcgaggct tcctcgccgc gtcccgccgt
     2041 gggctgcgcg gcgcggccgc ccacctcggc ggcgccgcta ccacccacgc gcgccgtcgc
     2101 ctcgcccgcg tggccgcgca gccgcgcgat catgaggtcg acgggcccac ccggcgccgc
     2161 ctgcacgatg aggaaatttg ccagcatgat cccgaacagc gtgggcacga tcagcagcag
     2221 gcggcgggcg aggtaggcga tcatccgttg ccgggaggcg acgtcttccg gggatcgatc
     2281 caccacgtgt cgaagccgag ctcgtacttc ggcgagacgg ccggacgccc gaagcggttc
     2341 cagtacgcca cgcggaacgc cgtgatgtgc cagtgcggga ccacgtagtg gctccacagc
     2401 aggacgcggt cgagcgcccg cgtccgcgcc accagcgacc gccggtccgg cgcggcgatg
     2461 agcgaggcga ccagggcgtc caccaccggg tcgcggatgc cggcgaggtt gcggctgccc
     2521 ggcgtgtccg cggcctccga gccccagaag tcggcctgct cgttgccggg cgagagtgac
     2581 tgggcgaaca ccgccaccgt cacgtcgaaa tcgaagtgct tcagccggta ctcgtactgc
     2641 gccgcgtcca ccgtgcgcag ccgcgcggcg atgcccagtc gctcgaggtt cttcacgaag
     2701 ggcagcgtga tgcgctccca gttcgcgtcc tcgatgagga tctcgaaggc catctggcgt
     2761 tccgtctccg cgttcaccag ccgcatgtcg cgcacgaccc agcccgcgtc cttcagcgtg
     2821 cgcagcgcct cgacgaggcc gggacgcaga ccgcccggcg cggtcgaggg cggacggtac
     2881 tccttcgtga agacctcgtc gggcacccgg ccccggaagg gcgccaggac cgccagctcg
     2941 tccggtcccg gcagcccccg tgcggccagc tcggagttcg agaaatagct gcgcgtgcgc
     3001 gtgtaggcgc cgtagaagag gtgcgcgttg gcccactcga agtcgaaggc gcgggccagg
     3061 gcctgccgca cccgcggatc cccgaacacg gggcgccgcg tgttgtagac gaagccctgc
     3121 atgcccgtgg ggacctcgtt ccggatctgc tccttcctga tgcgcccgtc gcgcacggcg
     3181 gggacgtcgt agcccctcgc ccagctcttc gcggagttct cctgccggaa gtcgtaggcg
     3241 ccggccttga acgcctcgag cgccacgatg ccgtcgcggt agtagtcata gcgaatcacg
     3301 tcgaagttga accggccgac gctcaccggc agctccgcgg cccagtagtc cggcacccgc
     3361 cggtaggtga tcgagcgacc gggctcgaac gactcgaccc tgtacgcccc gcttccgagc
     3421 ggcgcctcga gcgtggtctt ggcgaagtcg cgccccgccc agtacgcctt cggcagcacg
     3481 ggcatctggc ccacgatgag cggcagctcc cggttctcgc cgcgcttgaa ggtgaagagc
     3541 acgcggcgcg gcccggcctt ctcgacgctc gcgatctgcc ggtagtacga gcggtagaac
     3601 gggtgcccct tggccctgag cgtctcgaag gtccagatga cgtcctcgac cgtgaccggg
     3661 ctgccgtcgt ggaaccgcgc ctgcggacgc accgtgtagg ccacccacga ccggtccggc
     3721 ggcgtctcga tggtctcggc gacgagcccg tactccgaga acggctcgtc ggcggattgc
     3781 acggtgagcg agtcgaacac ggcgccgatc cccgccgccg ccacaccctt gaggatgaac
     3841 ggatggaggc tgtcgaaggt gccgatggcc gcgagcgtga ccttgccccc tctgggcgcc
     3901 cgcgggttca cgtagtcgaa gtgggcgaag ccgggagggt acttgaggtc gccgtgcatg
     3961 gaaaggccgt ggccggggcg gccggccgcg ggcgccgccc gcggccctgg agcggaggcc
     4021 gcaagcgcga ggagaagggc gacggcgacg gcgattctca tgccgtgtcg gacgtcaccg
     4081 agcatactcg cacagacgcc cctcaccgca agggaatgag gggaggagga cgacggctcc
     4141 cggtcagggc aggcggaaca gccggcgtgg attggactcg cc
//
LOCUS       DATIAZ010000135         4928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1085639, whole genome shotgun sequence.
ACCESSION   DATIAZ010000135 DATIAZ010000000
VERSION     DATIAZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085639"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VLF19_04695"
     CDS             <1..191
                     /locus_tag="VLF19_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92582.1"
                     /translation="ALAQEIGMRPQAARCHLGLGKLYRRPDKRELAQEHLATATTMCR
                     EMGMTYWLEKAEAAAASC"
     gene            complement(332..1306)
                     /locus_tag="VLF19_04700"
     CDS             complement(332..1306)
                     /locus_tag="VLF19_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSE92583.1"
                     /translation="MNVLLVGLGRWGEKHLRVLGETGATVWVADVSADRRAWALGQGI
                     APARVVDDYRAALPHVEAVDVVTPADSHLAIAETSLRAGRHCFVEKPLALTAAEGRRL
                     AAAAREAGRVLMPGHIFRFHPVTAALREALGAGCIGALRYATGRFAGFKGPRTDVGVT
                     HTDAIHYFDLFALLLGRAATRVSAVQRDFLGRGLDDMSVTVAHYGDVPAVVETNYFVP
                     GTCRECVLVGERGSLVADYGAATVMLHRGEFRRNGARWEAVDAGKDELPVGDREPLRA
                     ELETFRAACAGRIACPVPAEAGIAALEIVEAAALAARLDRTVEIEEVR"
     gene            complement(1314..2051)
                     /locus_tag="VLF19_04705"
     CDS             complement(1314..2051)
                     /locus_tag="VLF19_04705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSE92584.1"
                     /translation="MLDLSVVIPVYDEEDSLGPLWEELRPVLEGLALRWEVVFVDDGS
                     RDRSAEIVRGFRDRDARVRLVRLKANAGETAATDAGLKAARGRWVVTMDADLQNDPED
                     IPRLLAHLDRWDAVTGWRVDRAAGDSVVRRISSRVANSVRNRLSDEAIRDSGCTFRAF
                     RRECLRGLVLYRGFHRFVPTLLRMRGFRVLEVPVNHRPRRFGRSKYGVVNRAFVAFAD
                     LLAVRWMKSRLLSYEVAEDLGGDLLRE"
     gene            complement(2044..2640)
                     /locus_tag="VLF19_04710"
     CDS             complement(2044..2640)
                     /locus_tag="VLF19_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HSE92585.1"
                     /translation="MSRLVVAITGATGSIYGIRLLEVLRGYADMETHLVISRPGKRTL
                     VEETDWSVAEVEALATHRHENSDIGAPIASGSFRTAGMVVAPCSMKTAAAIATCHSEN
                     LVARAADVTLKEGRPLIMLVRETPLHVGHLKCMLALAEMGAVVLPPMPAFYNRPRDLD
                     DIVNHTIARVLDRLGLPQTLVPEWRGTHPRIPTGPLGA"
     gene            complement(2637..2906)
                     /locus_tag="VLF19_04715"
     CDS             complement(2637..2906)
                     /locus_tag="VLF19_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSE92586.1"
                     /translation="MSRERVFHLAEQIMKSFGTTPGVNIKAPDDLRPELIRVLSEESR
                     LEDSVDSEVRKILGSYARPMPEGSREWEVLYQKTREEVYKRRFRL"
     gene            complement(2912..3160)
                     /locus_tag="VLF19_04720"
     CDS             complement(2912..3160)
                     /locus_tag="VLF19_04720"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSE92587.1"
                     /translation="MADAVIKRLTVRKIVEVKDEPAARAAVRQIVVDNLAAEEQIEAD
                     ARKLLLDHAKTIKDSAADYRVLLGKVKEKLARERGFIL"
     gene            complement(3226..4713)
                     /locus_tag="VLF19_04725"
     CDS             complement(3226..4713)
                     /locus_tag="VLF19_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSE92588.1"
                     /translation="MTDVEGARGARVVWVATVALAAAGAVLQTVDPGPASPFRHLWTI
                     PVLAAGVRFGVHGGALAAVAAAMAQTPALFVHIENAGLSAAAVDDLVSCLTLLAAGPL
                     LGALAAEARLQRSRSALALAVPQALADEAPLPVALLRVKSLLERALAPAEIALVARVG
                     GEEALAGGPRVATGSAVAAVLAGAAAVFIPSIAGGLRPRRALVVPLVAHGEIVGALAV
                     ERAGELPARERAAIEALGVPLALALENARLAARQRRSAEELAEKVAAATRHLEIADRA
                     KSAFVAVASHELRSPLTALRGFSELLVLRAAAPGEVRRVAGIMQRETERLARIVEDLL
                     DLSRLERGLAPQLDPRPLSAATAITSGVEVFRDGTHRVMVACAEPLPDVLADPDALDR
                     VLKNLVSNAIKYSPPGTVVRVSARAAGGGVEIAVADQGRGIPADALPRLFEPYYRAPG
                     SEGARGTGLGLAVVKALVEAHGGTVSVESAPGTGTRVAFTLRSLS"
     gene            complement(4727..>4928)
                     /locus_tag="VLF19_04730"
     CDS             complement(4727..>4928)
                     /locus_tag="VLF19_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92589.1"
                     /translation="TTVASVARYLLDPRLPPTAEARVGAVVYLALLGAASAWLVARAG
                     SLVPALAASATFFAAYRLLGPP"
BASE COUNT          697 a         1823 c         1791 g          617 t
ORIGIN      
        1 tggccctggc gcaagagatc ggcatgcgac cgcaagcggc gcgctgccac ctcggcctcg
       61 gcaagctcta ccgccgcccg gacaagcgtg agctggccca ggagcatctc gccaccgcga
      121 cgacgatgtg ccgcgagatg ggcatgacgt actggctgga gaaggcggag gccgccgccg
      181 cttcctgctg atctcgctgg ccggtgccat cgccgcgccg ctcggcgccg gggcgcagca
      241 ggctgggagg atctatcgcg ttgcttggct cggctcgggc tctggaaggc cgcgtgggac
      301 aaggttcgac gttcacgtcg ccgctgctcg cctagcggac ttcctcgatc tcgacggtgc
      361 ggtcgaggcg cgcagcgagg gcggcggcct cgacgatctc cagcgcggcg atgccggcct
      421 cggcgggcac cgggcacgcg atgcgcccgg cgcacgccgc gcggaacgtc tcgagctccg
      481 cacgaagcgg ctcgcggtcg cccaccggca gctcgtcctt gccggcgtcg acggcctccc
      541 agcgggcgcc gtttctccgg aattcgccgc gatggagcat gaccgtggcg gcgccgtagt
      601 cggcgacgag gctgccgcgc tcgccgacga ggacgcactc gcgacaggtg ccgggcacga
      661 agtagttcgt ctcgaccacc gccgggacgt cgccgtagtg ggccaccgtc accgacatgt
      721 cgtcgagccc gcggccgagg aagtcgcgct gcaccgcgct gacgcgagtg gccgcgcgcc
      781 ccaggagcag ggcgaagagg tcgaagtagt ggatggcgtc ggtgtgcgtg acgccgacgt
      841 cggtgcgtgg ccccttgaac cccgcgaagc gcccggtggc gtagcgcagg gcaccgatgc
      901 agccggcgcc gagcgcctcg cgcagcgccg cggtgaccgg atggaagcgg aagatgtggc
      961 ccggcatcaa cactcgtccg gcctcccgcg ccgccgccgc gaggcgccgg ccctcggctg
     1021 ccgtcagcgc gagcggcttc tcgacgaagc agtggcgccc ggcgcgcagg ctcgtctccg
     1081 cgatggcgag atggctgtcg gccggcgtga ccacgtccac ggcctccacg tggggcagcg
     1141 ccgcgcggta gtcgtccacc acgcgcgccg gggcgatccc ttgcccgagc gcccacgcgc
     1201 gccgatcggc cgacacgtcg gcgacccaca cggtcgcgcc ggtctcgccc aggacgcgca
     1261 ggtgcttctc gccccagcgg ccgaggccga ccagcaggac gttcaccgag gggctactcc
     1321 cgcagcaggt cgccgccgag gtcctcggcg acctcgtagg agaggaggcg cgacttcatc
     1381 caccgcaccg ccagcagatc cgcgaacgcc acgaacgccc ggttcacgac gccgtacttc
     1441 gagcgcccga accgccgcgg ccggtggttc accgggacct cgagtacccg gaacccgcgc
     1501 atcctcagga gcgtcgggac gaagcggtga aagccgcggt acagcacgag cccgcgcagg
     1561 cactcgcggc gaaacgcgcg aaacgtgcag ccgctgtccc ggatcgcctc gtccgagagc
     1621 cggttgcgca cgctgttggc cacgcgggac gagatccggc gcacgaccga gtcgccggcg
     1681 gcccggtcca cgcgccagcc ggtgacggcg tcccaccggt cgaggtgggc gagcaaccgc
     1741 gggatgtcct cgggatcgtt ctgcaggtcc gcgtccatgg tgacgaccca ccgcccgcgc
     1801 gccgccttca agccggcgtc ggtggccgcc gtctcgccgg cgttcgcctt cagccggacg
     1861 aggcgcacgc gcgcgtcgcg gtcgcggaag ccccgaacga tctcggcgct gcggtcgcgg
     1921 ctgccgtcgt cgacgaagac gacctcccac cgcaacgcca gcccctcgag gaccggccgc
     1981 agctcctccc agagcgggcc gaggctgtct tcctcgtcgt agacggggat gacgaccgac
     2041 aggtcaagca ccgagtggtc cggtcgggat cctcgggtgc gtgccccgcc actccggaac
     2101 gagcgtctgg ggcaggccga gccggtcgag cacgcgggcg atcgtgtggt tgacgatgtc
     2161 gtccaggtcc cgcggccggt tgtagaacgc gggcatcggc ggcagcacca ccgcgcccat
     2221 ctcggcgagc gccagcatgc acttgaggtg cccgacgtgc agcggggtct cgcgcacgag
     2281 catgatcaag ggccggcctt ccttcagcgt cacgtcggcc gcgcgcgcca cgaggttctc
     2341 ggagtggcag gtggcgatgg ccgcagccgt cttcatggag cacggcgcga ccaccatgcc
     2401 cgcggtgcgg aacgagcccg aggcgatcgg cgcgccgatg tcgctgttct cgtgacggtg
     2461 cgtggccagg gcctcgacct cggccaccga ccagtcggtc tcctcgacga gggtccgctt
     2521 gcccggccgc gagatcacca ggtgggtctc catgtcggca tacccgcgga gcacctcgag
     2581 caggcggatg ccgtagatcg agccggtggc gccggtgatg gcgacgacga gccggctcac
     2641 aggcggaacc tgcgcttgta aacctcctcg cgggtcttct ggtagagcac ctcccactcg
     2701 cggctgccct cgggcatcgg gcgggcgtag gagccgagga tcttgcgcac ctcggaatcg
     2761 accgagtcct cgagccgcga ctcctcgctg agcacgcgga tgagttccgg tcgcagatcg
     2821 tccggggcct tgatgttgac ccccggcgtc gtgccgaaac tcttcatgat ctgctcggcg
     2881 aggtggaaga cgcgctcccg gctcatccgc atcagaggat gaatccccgc tcgcgggcga
     2941 gtttctcttt caccttgccc agcagcacgc ggtagtcggc ggccgaatcc ttgatggtct
     3001 tggcgtgatc gaggagcagc ttgcgcgcat cggcctcgat ctgctcctcc gcggcgagat
     3061 tgtcgacgac gatctgacgg acggcggcgc gcgcggccgg ctcgtccttg acctcgacga
     3121 tcttccgcac ggtgaggcgc ttgatcaccg catcggccat cctctcggcg atctcgtcgc
     3181 gcctaggcat agcccacgta ctctacccgc tcgtgacgaa gagtgtcaag aaagcgaccg
     3241 gagcgtgaac gccacgcggg tgcccgttcc cggcgcgctc tcgacgctca ccgtcccgcc
     3301 gtgagcctcg acgagcgctt tcaccacggc gaggcccagc cccgtgcccc gtgcgccctc
     3361 gctgcccggc gcacggtagt acggctcgaa cagacgcggc agcgcgtcgg cgggaatgcc
     3421 ccggccctgg tcggcgaccg cgatctccac gcccccgccc gcggcgcgcg cgctgacacg
     3481 caccacggtg cccggcggcg agtacttgat ggcgttggag acgaggttct tcagcacgcg
     3541 gtcgagcgcg tcggggtcgg ccaggacgtc gggcagcggc tccgcgcacg cgaccatcac
     3601 gcggtgcgtg ccgtcgcgga acacctcgac gccgctggtg atcgccgtcg ccgccgagag
     3661 cggacgcgga tcgagttgcg gcgccagccc gcgctcgagg cgcgagaggt cgagcaggtc
     3721 ctcgacgatg cgcgccagcc gctccgtctc gcgctgcatg atgccggcga cgcggcgcac
     3781 ctcgccgggg gcggcggcgc gcaggacgag cagctcgctg aagccgcgca gcgccgtcag
     3841 cggcgagcgc agctcgtgcg acgccacggc cacgaacgcc gacttcgccc ggtcggcgat
     3901 ctctagatgg cgcgtcgccg ccgcgacctt ctcggcgagc tcctcggccg accggcgctg
     3961 gcgcgccgcg agccgcgcgt tctccagcgc cagcgcgagg ggcacgccga gcgcctcgat
     4021 cgcggcccgc tcccgcgcgg gcagctcgcc ggctcgctcg acggccagcg cgccgacgat
     4081 ctcgccgtgg gccaccaggg gcaccaccag cgcccgccgc ggccggaggc cgcccgcgat
     4141 gctcgggatg aacaccgcgg cggcgcccgc gagcaccgcg gcgacggccg atccggtggc
     4201 gacgcgaggg ccgccggcga gcgcctcctc gccgccgacg cgcgcgacca gcgcgatctc
     4261 ggcgggtgcc agcgcgcgct cgagcaggct cttcacccgc aggagcgcca ccggcagggg
     4321 cgcctcgtcg gcgagcgcct gcggaacagc gagcgccagg gcggagcgcg agcgctggag
     4381 gcgggcctcg gcggccagcg cgccgaggag gggcccggcg gcgagcagcg tgagacacga
     4441 gacgagatcg tcgacggcgg cagcgctcag ccctgcgttc tcgatgtgga cgaagagggc
     4501 cggcgtctgc gccatcgcgg ccgcgacggc ggcgagcgcg ccgccgtgga cgccgaagcg
     4561 caccccggcg gcgagcacgg ggatcgtcca gaggtgacga aacggcgagg ccggacccgg
     4621 atcgaccgtc tgcagcaccg cgccggccgc ggccagcgcc accgtggcga cccagacgac
     4681 gcgggcgcct cgcgcaccct ccacgtccgt cacgcggccg ccgcgctcac ggcgggccga
     4741 gcaggcggta ggcggcgaag aacgtggccg aggctgcgag ggccggcacc agactgccgg
     4801 ctctggccac gagccacgcg ctcgcggcgc cgagcagcgc gagatacacg accgccccga
     4861 cccgtgcctc cgccgtgggc ggcagtcgcg ggtcgagcag atagcgagcg acgctcgcca
     4921 ccgtggtc
//
LOCUS       DATIAZ010000136        10088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1217377, whole genome shotgun sequence.
ACCESSION   DATIAZ010000136 DATIAZ010000000
VERSION     DATIAZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217377"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VLF19_04735"
     CDS             complement(<1..200)
                     /locus_tag="VLF19_04735"
                     /inference="COORDINATES: protein motif:HMM:NF018654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HSE92590.1"
                     /translation="MPRRPSSRLPKVPEMTIRRLSVYTRCLLQLEEDGVKTVSSQELA
                     ERFNLNSAQVRKDLAYFGEFGVR"
     gene            complement(259..588)
                     /locus_tag="VLF19_04740"
     CDS             complement(259..588)
                     /locus_tag="VLF19_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSE92591.1"
                     /translation="MRRSLIAVGSIVLGSPGLALAQAPAAQAGSSWVGAFAVVAAAIG
                     MAIAAGLCGLGQGRAVASAVDAMARQPGAAARIQTAMIIGLALIESLAIYVLVVAMIL
                     LFVQPIK"
     gene            complement(617..1369)
                     /gene="atpB"
                     /locus_tag="VLF19_04745"
     CDS             complement(617..1369)
                     /gene="atpB"
                     /locus_tag="VLF19_04745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSE92592.1"
                     /translation="MGAIEHPPVISLPGIPDHVTYTWLVMIILAGVAFAATRNVALVP
                     RGVQNFMEVVLEQFVSLIDDVIGHDGRRFLPLIATLGLFILTGNLLGIVPGVAGPTAN
                     LNTTAACALVVFVAYHAIGIRAQGPVNYLRHFMGPVPWLAPLMFPIELISHLARPLSL
                     TLRLFGNMFGGHILLAIIFFLMGLDGLIGWALSGSAAGVLIGGVGGVIMVVFTVVFLY
                     PLKILVAFLQAFIFVMLTMLYISGALEDAEHH"
     gene            complement(1373..1741)
                     /locus_tag="VLF19_04750"
     CDS             complement(1373..1741)
                     /locus_tag="VLF19_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HSE92593.1"
                     /translation="MTTTELSARVTVESAVACAALALLALLGWGVTAGAGVAAAGALT
                     IVNFRWLARAAALAAAGGRERRLAAWGLSAAGRFAGVLAAFALLFTSGWVHPVGVVVG
                     LAVLPCAVVARGLAAARVER"
     gene            complement(1728..1961)
                     /locus_tag="VLF19_04755"
     CDS             complement(1728..1961)
                     /locus_tag="VLF19_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSE92594.1"
                     /translation="MTSGGEPAWRSVGELASIGMAMVLATVIGLGAGYYADRWLGTSP
                     WLTLIGLGLGIAAGFVTLFRSVKAAERNLDDDD"
     gene            complement(2068..3798)
                     /locus_tag="VLF19_04760"
     CDS             complement(2068..3798)
                     /locus_tag="VLF19_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSE92595.1"
                     /translation="MTDAVSAGVEGTSGSSRARPTRDDRLAGTLGVGIIWLFLIVFLV
                     YPLVRIFYDAVTDEAGAFTLRHLGEFVGDAYYRRSLVNSLLLGLGTVAATSVLGFAVA
                     FLLVRYDFAGRGLFSYLTLIPIISPPLVGVLGFTFILGRAGTVNVLLHDWFEVLRPLN
                     FVYGVHGVLLVETLHLFPMITLNVVDALGKIDPALEEAAESVGARGWRKLRTVTLPLT
                     TPGYVAGALLVFIWTFSDFATPLVLGVHDLLAAQAYLNIVQFVDRRLFRMGIVISALM
                     VVLALVFLVAARRYVAIKDYSSLSYSRIARRRLSGPKQALAVAFLALVLLASFIPYLG
                     VGLASVGRGWSLTPFPVQYTLLYFERVIVETPKYVLNSLLYAGLAVALCIAVGVPIAW
                     LLARTRVPGRDTLDGLNTLILAIPGTAIGIAYIRAFHVDLPWLERPLTSFWIILPLVL
                     AIRRLPYTVRGSYASLLLVHRSMEEAAASVGAGGLRAFRDVTLPLIWRGVLVGSLFSF
                     MTSLQEASAVLFLSLGGWETMTVGIFSFYIAGSANEAAALGVILIVVAAVSVIAINRI
                     AGTRMGGMFG"
     gene            complement(3799..4860)
                     /locus_tag="VLF19_04765"
     CDS             complement(3799..4860)
                     /locus_tag="VLF19_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92596.1"
                     /translation="MKIALRSVLKRFGGVTAVDRVGLDIGDGELFTLLGPSGCGKTTL
                     LRLVAGFYAPDGGEIWFGDRRVDGLAPYQRNIGMVFQNYALWPHMTVRGNVTYGLRLK
                     RLAAAEVDRRLAEGLRQVNLVGLEHRYPGQLSGGQQQRVALARVLVLNPDILLLDEPL
                     SNLDAKIRVQVRAEIRRLQRDLGITTIYVTHDQEEALSLSDRVAVMRDGRVLQVAAPK
                     DLYERPATRFVADFVGVNNFIPGVCREAADGVVVVETPLGAICGRRGGAVRAGDRCVV
                     AVRPENVALGGGGDNVFPGRVVLASYLGSTLRYDVEANSNLLVKVDIRDPWHHEPLAA
                     GAQVTLSFPASTALTLAED"
     gene            complement(4910..6040)
                     /locus_tag="VLF19_04770"
     CDS             complement(4910..6040)
                     /locus_tag="VLF19_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE92597.1"
                     /translation="MARLCYLGRLPVLVLAATVALGLAGPAVAQAPIEGELTLITPVS
                     KFIHDAALKAFAEYAKEKWNVTVRTNAIPAGTPVAYGRIVEWKGKPEADIFWGGESAL
                     FEKLAEQKLLQKVEISKEAWESIPAAIGKPKPIPLKDKDGFWVGTALEPYGLVYHPKR
                     IQRLGVPEPKEWEDLLDPKLKGEVAQCAPTRSSSSNATYEVILSMHGEDKGWAWLKRL
                     AQNTGHFTARSRDVPTVVAKGEYAAGFAVPSYMAFEEKLAGFDIKFVAPKNAFVTPEP
                     MAILAGSRNPKAARAFIEFLLTERGQRVFMERGLFPITPKYKVQGPPGSTAELAVQFT
                     GGVRSYFDGDISNVYDEAIAAKRSEALKTKYRSDIEAVWKKP"
     gene            complement(6127..7629)
                     /gene="glpK"
                     /locus_tag="VLF19_04775"
     CDS             complement(6127..7629)
                     /gene="glpK"
                     /locus_tag="VLF19_04775"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HSE92598.1"
                     /translation="MSTRRHVLALDQGTTGSTALVVDPDGRVRARGYAELSQHFPRPG
                     WVEHEPEEIWTTVEQAASAALKGAGIAGGEVAAVGITNQRETTVLWDRASGAPIHRAI
                     VWQCRRTADTCERLRARGCEPLVRERTGLVLDAYFSATKIGWLLDAVPDARQRARRGE
                     LAFGTVDSWLLWKLTGGRVHATDVSNASRTLLFGLRAVDWDAEMLALFEVPREVLPRV
                     LASSGVFGETTDLGWLPRGVPIAGIAGDQQAALFGQACFDPGSAKNTYGTGCFLLLNT
                     GKTPVASTRGLVTTIAWRLDGRTTYALEGSVFVAGAAVQWLRDGLGLLNTAAESQALA
                     ESVPDTGGVYLVPAFVGLGAPHWDMYARGAIVGLTRGTTAAHLTRAALEAIAYQSRDV
                     LEAMVADAGIAVRAMRVDGGAAANDFLCQFQADITDVPVLRPAVIETTALGAAYLAGL
                     GAGVWRSLDGVAARASVERTFTPAMDATTRIRRYEGWRRAVELAKGWAQP"
     gene            complement(7712..8545)
                     /locus_tag="VLF19_04780"
     CDS             complement(7712..8545)
                     /locus_tag="VLF19_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HSE92599.1"
                     /translation="MRRGFRLALLGLALTAAVAAGPVPGRPLLAAHRGGGLLWPENSL
                     LAYRNALGLDVDYLETDVHLTADGEVVVIHDPTLERTSTGIGALRERTLADLGPVRLK
                     TRDGGVTDERVPTLAQLLDLVAPSRAGLLLEIKVDERKQRHPGIEEKVLALVRSRRME
                     GRTLVMAFERETLRHVRELDPAIRTVLLIGRGEVQRERVWPAESVKWAAGLGATAIGY
                     NHRLIDADVVAAAGKAGLMLAAWTVNEEADVRRMLDLGVDVVISDRPDLVRRLVDRPK
                     P"
     gene            complement(8542..9345)
                     /locus_tag="VLF19_04785"
     CDS             complement(8542..9345)
                     /locus_tag="VLF19_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HSE92600.1"
                     /translation="MSPRFVAHRGGAALWPENSLLAFRSAIALGAGLLELDVHGTADG
                     GVAVIHDPVLDRTTDARGPVGARTLIELRRTRLRGPDGRLTDEWVPAFDEVLALAAPA
                     GVTLLIEIKTPGPAVAYERGAAGFRAVPGPRYEGLERRVLEALTRAGMDGRAFVLAFS
                     PAVIAEVRELAPGQPTALLVDRDHVLASGAPPAAAVDWACQARANFLGLHYTLCDGDV
                     VGAARAAGLALGVFTVNDEVEMRRLAGLGVDVIISDRADLVQRLSAEVA"
     gene            complement(9342..>10088)
                     /gene="ugpE"
                     /locus_tag="VLF19_04790"
     CDS             complement(9342..>10088)
                     /gene="ugpE"
                     /locus_tag="VLF19_04790"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HSE92601.1"
                     /translation="YAFVISTQSLEEVIQRPPVLVPSTHAVENYLEAWRRSGMGRLLV
                     NSAIVAVTVAVGKITISLLSAFAIVYFPFRGRQLVFWMIFITLMLPIPVRILPTYEVI
                     GDLGWLNSYAGLTVPLMASATATFLFRQFYLTVPNELCEAAQLDGCGPLRFLWSFLLP
                     LSWANIAALFVVLFIYGWNEYFWPLLITNTDDMRTVVIGLERLIPRSGTELPTWNLIM
                     AGAMMALLPPVAVIVFMQRWFVRGLIESEK"
BASE COUNT         1629 a         3600 c         3558 g         1301 t
ORIGIN      
        1 cgcacgccga actcgccgaa gtacgcgagg tccttccgca cctgggccga gttcaggttg
       61 aagcgctcgg ccagctcctg cgaggacacg gtcttcaccc cgtcctcctc gagctgcagg
      121 aggcagcggg tgtagacgga gagacgacgg atcgtcatct ccgggacctt cgggagccgg
      181 gagctcgggc gccggggcat gggatccgtc cggcgggcgc gcgccgtgtg gggtcgggcg
      241 cccgccgggc gccgatcgtt acttgatagg ctgcacgaac agcaggatca tcgccaccac
      301 gagcacgtag atcgcgagcg actcgatgag ggccagaccg atgatcatcg cggtctggat
      361 gcgggctgcc gcgcccggct gccgcgccat ggcgtcgacg gcggaggcca ccgcgcggcc
      421 ctggccgagg ccgcacagcc cggcggcgat cgccatgccg atcgcggcgg cgacgaccgc
      481 gaacgcaccg acccaactac ttccggcctg ggccgccggc gcctgcgcga gcgccaagcc
      541 aggggacccc aagacaatgg aaccaaccgc gatcagtgaa cgtcgcacgc ccgtcctcct
      601 ctgtacacgt gatgtgctag tggtgctcgg cgtcctcgag ggctccggag atgtagagca
      661 tcgtcagcat gacaaagatg aaggcctgca gaaaggcgac caggatcttc agcggataga
      721 ggaagaccac ggtgaacacc accatgatca cgccgcccac tccaccgatc aggaccccgg
      781 ccgcgctgcc cgagagcgcc cagccgatga gaccgtccag gcccatgagg aagaagatga
      841 tggcgagcag gatgtgaccg ccgaacatgt tgccgaagag cctgagcgtc agcgaaagcg
      901 gccgggcgag gtggctgatc agctcgatgg gaaacatgag gggcgccagc cagggcaccg
      961 gtcccatgaa gtgcctcagg tagttcaccg ggccttgcgc gcggatgccg atggcgtgat
     1021 aggcgacgaa gaccaccagc gcgcacgcgg ccgtcgtgtt caggttcgcc gtcggcccgg
     1081 cgacgccggg cacgatgccg agcaggttgc cggtcaggat gaagagcccc agggtggcga
     1141 tgagcggcag gaaccggcgg ccatcgtgcc cgatgacgtc gtcgatcagg ctcacgaatt
     1201 gctcgaggac gacctccatg aagttctgca cgccgcgcgg caccagggcc acgttccgcg
     1261 tggccgcgaa ggccaccccg gcgaggatga tcatgaccag ccacgtgtag gtgacgtggt
     1321 cgggaatgcc cggcagcgag atgaccggcg ggtgttcgat cgcgcccatc gatcagcgct
     1381 ccacgcgcgc cgccgcgagg ccgcgggcga cgacggcgca gggcagcacg gcgagcccca
     1441 ccacgacccc gacgggatgg acccagcccg aggtgaacag caaggcaaag gccgcgagca
     1501 cgccggcgaa gcgcccggcc gccgagagac cccacgcggc cagccggcgc tcgcgcccgc
     1561 ccgccgcggc cagggccgcg gcgcgcgcga gccaccggaa gttgacgatc gtcagcgcgc
     1621 cggcggcggc gacgccggca ccggccgtga caccccaccc caggagtgcg agcagggcca
     1681 gcgcggcgca ggccacggcg ctctcgaccg tcacgcgcgc actcaactca gtcgtcgtca
     1741 tcgaggttcc gctcggcggc cttcaccgat cgaaagagag tcacgaagcc ggcagcgatg
     1801 ccgagcccca agccgatcag ggtcagccac gggctcgtcc ccagccaccg atccgcgtag
     1861 tagccggcgc cgagaccgat cacggtcgcg agcaccatgg ccatgccgat cgaggccagc
     1921 tcgccgacgg acctccaggc cggctcgccg cccgacgtca tgtgaacacg gacactagca
     1981 catgggcaat gccggggcaa gggtcctcaa gtagcggatt tacttgtcgt ttatttgtgg
     2041 gtcgccgggg gggtgaggca caatcgtcta cccgaacatg ccgcccatgc gcgtgccggc
     2101 gatgcggttg atcgcgatca cgctcacggc cgcgaccacg atcaggatga cgccgagcgc
     2161 agccgcctcg ttggcgcttc ccgcgatgta gaacgagaag atgccgaccg tcatcgtctc
     2221 ccagccgccg agtgagagga acagcaccgc ggaggcttcc tgcagcgacg tcatgaacga
     2281 gaagagagag ccgacgagca cgccacgcca gatgaggggc agcgtgacgt cgcggaacgc
     2341 gcgcagaccg ccggccccga cactggccgc ggcctcctcc atcgagcggt gcacgagcag
     2401 cagcgacgcg taggagccgc gcacggtgta cggcagacga cggatggcga gcacgagggg
     2461 caggatgatc cagaagctcg tcagcgggcg ctcgagccac ggcaggtcca cgtgaaaggc
     2521 gcggatgtag gcgatgccga tggcggtacc gggaatggcg aggatcagcg tgttgagccc
     2581 gtcgagcgtg tcgcgccccg gcacgcgcgt tcgcgcgagc agccaggcga tgggcacgcc
     2641 gacggcgatg cagagcgcga cggcgaggcc ggcgtagagg aggctgttca ggacgtactt
     2701 cggtgtctcc acgatcacgc gctcgaagta cagcagcgtg tactgcaccg ggaacggtgt
     2761 cagcgaccag ccccggccga cggaagccag cccgactccg aggtacggga tgaacgacgc
     2821 cagcagcacc agcgcgagga acgcgaccgc gagcgcctgc ttcggccccg acagccgccg
     2881 gcgggcgatg cgtgagtacg acagggacga gtagtccttg atggccacgt accgccgggc
     2941 ggcgacgagg aacaccagcg cgagtacgac catcagggcc gagatgacga tgcccatgcg
     3001 gaacaggcgc cggtcgacga actgcacgat gttgaggtac gcctgcgcgg ccagcaggtc
     3061 gtggacgccg aggaccagcg gcgtcgcgaa atccgagaac gtccagatga agaccagcag
     3121 agcgccggcg acgtacccgg gcgtggtcag cggcagggtg accgtccgga gcttgcgcca
     3181 cccgcgcgcg ccgacgctct cggcggcctc ctccagcgcg gggtcgatct tgccgagcgc
     3241 gtccacgaca ttgagcgtga tcatcgggaa gaggtgcagg gtctccacga gcagcacgcc
     3301 gtggacgccg tacacgaagt tgagcggccg gagcacctcg aaccagtcgt ggagcagcac
     3361 gttcacggtg cccgcccgcc cgaggatgaa ggtgaacccg agcacgccca cgagtggcgg
     3421 cgagatgatc gggatcagcg tcaggtagct gaagagtccc cgcccggcga agtcgtagcg
     3481 gaccaggagg aaggcgacgg cgaagcccag gaccgacgtg gcggccacgg tgccgagccc
     3541 gagcagcagc gagttgacga gcgaccgccg gtagtaggca tcgcccacga actcgccgag
     3601 gtggcggagg gtgaaggcgc cggcctcgtc ggtgacggcg tcgtagaaga tgcggacgag
     3661 cgggtagacg aggaacacga tcaggaagag ccagatgatg ccgacgccga gcgtcccggc
     3721 caggcggtcg tcgcgggtgg ggcgggcccg gctcgagccg gacgtcccct ccacgcccgc
     3781 gctgacggcg tcggtcactc agtcctcggc cagcgtcagc gccgtcgagg ccgggaaact
     3841 cagcgtcacc tgggccccgg ccgcgagcgg ctcgtggtgc caggggtcgc ggatgtccac
     3901 cttcacgagg aggttactgt tcgcctcgac gtcgtaacgc agcgtgctgc ccagatacga
     3961 cgccagcacc acgcggccgg ggaagacgtt gtcgcccccg ccgcccagcg cgacgttctc
     4021 gggacgcacc gccaccacgc agcggtcgcc ggcgcgcacc gcccccccgc ggcggccgca
     4081 tatggcgccc agcggcgtct cgaccacgac gacgccgtcg gccgcctcgc ggcacacgcc
     4141 ggggatgaag ttgttgacgc cgacgaagtc cgccacgaag cgcgtggcgg ggcgctcgta
     4201 cagatccttg ggcgcggcca cctggagcac ccggccgtcc cgcatcacgg caacccggtc
     4261 ggagagcgac agcgcctcct cctgatcgtg ggtgacgtag atggtggtga tcccgaggtc
     4321 gcgctgcagc cgccggatct ccgcacggac ctgcacgcgg atcttcgcgt cgaggttcga
     4381 gaggggctcg tcgaggagga ggatgtcggg gttcagcacg agcacccgcg ccagcgccac
     4441 gcgctgctgc tggccgccgg agagctggcc cgggtagcgg tgctcgagcc cgacgaggtt
     4501 gacctgccgc agcccctcgg cgagccgccg gtcgacctcg gccgccgcga gccgcttgag
     4561 ccggagcccg taggtgacgt tgccgcgcac cgtcatgtgc ggccagagcg cgtagttctg
     4621 gaacaccatg ccgatgtttc gctggtaggg cgccagcccg tccacccggc ggtcgccgaa
     4681 ccagatctcg ccgccgtcgg gcgcgtagaa gccggcgacg agccgcagca gtgtcgtctt
     4741 gccgcacccc gacggcccca gcagcgtgaa cagctccccg tcgccgatgt cgagaccgac
     4801 gcggtcgacc gccgtcacgc cgccgaaccg cttcagcacg gaccgcagcg cgatcttcat
     4861 cgacgtccga ttcggacggc cgccgcgcgc gagcccgcca ccgagcgcgt tacggcttct
     4921 tccacaccgc ctcgatgtcg gaccggtact tcgtcttcaa ggcctcggac cgcttggccg
     4981 cgatcgcctc gtcgtacacg ttcgagatgt cgccgtcgaa gtacgagcgg acgccgccgg
     5041 tgaactgcac ggccagctcc gccgtcgatc cgggcgggcc ctgaaccttg tacttcgggg
     5101 tgatcgggaa gaggccgcgc tccatgaaca cacgctgccc gcgctcggtg aggaggaact
     5161 cgatgaaggc gcgcgccgcc ttcgggttgc gcgagccggc gaggatggcc atgggctccg
     5221 gcgtcacgaa ggcgttcttc ggggccacga acttgatgtc gaagccggcc agcttctcct
     5281 cgaaggccat gtaggatggg acggcgaacc ccgccgcgta ctcgcccttg gccacgaccg
     5341 tcggcacgtc gcggctgcgc gcggtgaagt gaccggtgtt ctgggcgagc cgcttgagcc
     5401 acgcccagcc cttgtcttct ccgtgcatcg acaggatgac ctcgtaggtc gcgttcgacg
     5461 acgacgagcg cgtcggcgcg cactgcgcca cctcgccctt gagcttcggg tccagcaggt
     5521 cctcccactc cttcggctcc ggcacgccca gccgctggat gcgcttgggg tggtacacga
     5581 gcccgtaggg ctccagcgcc gtgcccaccc agaaaccgtc cttgtccttc agcgggatgg
     5641 gcttgggctt gccgatggcc gccgggatcg actcccaggc ctccttcgag atctccacct
     5701 tctgcaggag cttctgctcg gccagcttct cgaacagcgc cgactccccg ccccagaaga
     5761 tgtcggcctc gggcttgccc ttccactcga cgatccgacc gtaggcgacc ggcgtgccgg
     5821 ccgggatggc gttggtcctg accgtgacgt tccacttctc cttcgcgtac tccgcgaacg
     5881 ccttcagcgc ggcgtcgtgg atgaacttcg agaccggagt gatcagcgtc agctcgccct
     5941 cgatcggcgc ctgcgccacc gccgggcccg cgagcccgag cgccaccgtc gccgccagca
     6001 cgagtaccgg aagcctgccg aggtaacaga ggcgcgccac ggctatgccg gggcgtgccg
     6061 aacgtcgggg ggtttggggg ccatttcgtg gcccccgtgt catcagtcgt cgcatgggtc
     6121 ctcccctcat ggttgtgccc agcccttcgc gagctccacc gcccgccgcc agccctcgta
     6181 gcggcggatc ctcgtcgtcg cgtccatggc cggcgtgaac gtccgctcga ccgacgcccg
     6241 cgcggcgaca ccgtcgagcg accgccacac gccagcgccg agccccgcca ggtatgcggc
     6301 gccgagcgcc gtcgtctcga tcaccgcggg ccgtagcacc gggacgtcgg tgatgtcggc
     6361 ctggaactgg cagaggaagt cgttggccgc cgctccgccg tccacccgca tcgcccgcac
     6421 cgcgatcccg gcgtcggcta ccatcgcctc cagcacgtcc cggctttggt aggcgatggc
     6481 ctccagcgcc gcgcgcgtca ggtgcgccgc cgtcgtgccc cgcgtgaggc cgacgatggc
     6541 accccgcgcg tacatgtccc agtgcggcgc gccgaggccg acgaacgccg gcacgagata
     6601 gacgccgccg gtgtcgggta ccgattcggc gagcgcctgg ctctccgctg ccgtgttcag
     6661 caggcccaga ccgtcccgca gccactgcac cgcggcgccg gcgacgaaca cgctgccctc
     6721 gagcgcgtac gtcgtgcgcc cgtcgagtcg ccaggcgatc gtcgtcacca ggccccgcgt
     6781 ggaggccacg ggggtcttgc cggtgttgag cagcaggaag cagccggtcc cgtacgtgtt
     6841 cttcgcgctc cccggatcga agcacgcctg gccgaagagc gccgcctgct gatcgccggc
     6901 gatgccggcg atcggcacgc cgcgcggcag ccagcccagg tccgtcgtct cgccgaagac
     6961 gcccgacgag gccagcacgc ggggcaggac ctcgcgaggc acctcgaaga gggcgagcat
     7021 ttcggcgtcc cagtccacgg cccgcaggcc gaacagcagc gtacgggacg cgttgctgac
     7081 gtcggtggcg tgcacccggc cgccggtgag cttccacagg agccacgagt ccacggtacc
     7141 gaacgccagc tcgccgcggc gggcccgctg gcgcgcgtcg ggcacggcgt cgagcagcca
     7201 accgatcttg gtggcggaga agtaggcgtc gagcacgagg cccgtccgct cgcggacgag
     7261 cggctcgcag cccctggccc gcagtcgctc gcacgtgtcg gcggtgcgcc ggcactgcca
     7321 cacgatcgcg cggtggatcg gcgcgccgct ggcccggtcc cacagcacgg tcgtctcgcg
     7381 ctggttcgtg atgccgacgg ccgcgacctc gccgcccgcg atgccggcgc ccttcagggc
     7441 ggccgacgcg gcctgctcga ccgtcgtcca gatctcctcg ggctcgtgct ccacccaccc
     7501 cggacgcggg aagtgctggg acagctcggc gtagcctcgg gctcgcacgc gtccgtcggg
     7561 atcgaccacg agcgccgtcg agcccgtcgt gccctggtcg agcgccagga cgtggcggcg
     7621 cgtgctcatg gcgcgcgccg gtcgagcgcc aggccagggc ggcgcgcgct cgtggctcgc
     7681 cgccggtcga cgatgactgc gtatgacgcg ctcacggctt cgggcgatcg accagccggc
     7741 gcacgaggtc cggccggtcg ctgatgacga cgtccacgcc gaggtcgagc atccggcgga
     7801 cgtcggcctc ctcgttgacg gtccacgcgg ccagcatgag gccggccttg cccgccgcgg
     7861 ccaccacgtc ggcgtcgatc aatcggtggt tgtagccgat ggccgtggcg ccgaggcccg
     7921 cggcccactt cacgctctcg gcgggccaga cccgctcgcg ctgcacctcg ccgcggccga
     7981 tcagcagcac ggtacggatc gccggatcca gctcgcgcac gtgccgcagc gtctcccgct
     8041 cgaaggccat gaccagcgtg cgcccttcca tcctgcgcga ccggaccagc gccagcacct
     8101 tctcctcgat gccgggatgg cgctgcttgc gttcgtccac cttgatctcc aggagcaggc
     8161 cggcgcgcga aggcgccacg aggtccagca gctgcgccag cgtcggcacg cgctcgtcgg
     8221 tgacgccccc gtcccgtgtc ttgagcctca ccgggcccag gtcggccagc gtccgctcac
     8281 gcagcgcgcc gatgccggtg ctcgtgcgct cgagcgtcgg gtcgtggatc acgaccacct
     8341 cgccgtcggc ggtgaggtgc acgtcggtct ccaggtagtc cacgtcgagg ccgagcgcgt
     8401 tgcggtaggc gagcaggctg ttctcgggcc agagcaggcc gcccccccgg tgcgcggcca
     8461 gcaggggtcg gccggggacg ggcccggcgg ccacagccgc cgtcagggcg agccccagca
     8521 gcgccaggcg gaacccacgt ctcacgcgac ctccgcggac aggcgctgga cgaggtcggc
     8581 acggtcgctg atgatcacgt ccactccgag gccagcgagc cggcgcatct ccacctcgtc
     8641 gttcaccgtg aacacgccga gcgccaggcc cgccgcgcgc gcggcgccca cgacgtcgcc
     8701 gtcgcagagc gtgtagtgca ggcccaggaa gttcgcgcgg gcctggcacg cccagtcgac
     8761 cgccgccgcc ggcggcgcgc cggaggcgag cacgtggtcc cggtcgacca gcagcgccgt
     8821 cggctgcccg ggcgcgagct cccggacctc ggcgatgacc gccgggctga acgcgagcac
     8881 gaacgcccgg ccgtccattc cggcgcgcgt gagcgcctcg agcacgcggc gctcgagccc
     8941 ctcgtagcgc ggccccggaa cggcccggaa gccggcggcc ccgcgctcgt aggccacggc
     9001 cggccccggc gtcttgatct cgatcagcaa ggtcacgccc gccggcgccg ccagcgccag
     9061 cacctcgtcg aaggccggca cccactcgtc ggtgagccgg ccgtcggggc cgcgcagccg
     9121 cgtccggcgc agctcgatca gcgtccgggc gccgaccggg ccgcgcgcgt cggtggtgcg
     9181 atcgaggacg gggtcgtgga tgacggccac cccgccgtcc gcggtgccgt gcacgtccaa
     9241 ctcgagcagg cccgcgccga gcgcgatcgc gctgcggaag gcgagcaggc tgttctccgg
     9301 ccagagggcg gcgccgccgc ggtgggcgac gaagcgcggg ctcacttctc gctctcgatc
     9361 aggccccgca cgaaccagcg ctgcatgaag acgatcacgg ccaccggcgg cagcagggcc
     9421 atcatggccc cggccatgat gagattccag gtcggcagct ccgtgcccga gcgcgggatg
     9481 agccgctcga gcccgatgac gaccgtgcgc atgtcgtcgg tgttggtgat gagcaggggc
     9541 cagaagtact cgttccaccc gtagatgaag agcaccacga acagggccgc gatgttggcc
     9601 cacgagagcg gcagcaggaa cgaccagagg aaccgcaacg gcccgcagcc gtcgagctgg
     9661 gcggcctcgc agagctcgtt gggcacggtg aggtagaact gccggaacag gaacgtcgcc
     9721 gtggccgagg ccatgagcgg caccgtgagg cccgcgtagc tgttgagcca gccgaggtcg
     9781 ccgatgactt cgtaggtcgg caggatccgg accggaatcg gcagcatgag cgtgatgaag
     9841 atcatccaga acacgagctg ccggccccgg aagggaaagt acacgatggc gaaggcggac
     9901 agcagcgaga tcgtgatctt gccgacggcc acggtcacgg ccacgatcgc ggagttcacc
     9961 aggagccggc ccatccccga gcgccgccag gcctccaggt agttctcgac ggcgtgcgtg
    10021 gacggcacca gcaccggcgg ccgctggatc acctcctcca ggctctgggt ggagatcacg
    10081 aaggcgta
//
LOCUS       DATIAZ010000137        11794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_428509, whole genome shotgun sequence.
ACCESSION   DATIAZ010000137 DATIAZ010000000
VERSION     DATIAZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11794
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428509"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1191)
                     /locus_tag="VLF19_04795"
     CDS             complement(37..1191)
                     /locus_tag="VLF19_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE92602.1"
                     /translation="MAKGSFKVMDSDIHVTEPRDVWSRYIEPRFKDRAPRFEPIEGSH
                     VEGWQFEGMAFPAFINRPERRRLASIRREKARARHVATGRYRDPKEDLKGNDPGAMLQ
                     AMDREGIDVSIVFRTMGSHVVAIDGLDPELSAAVCRAFNNWLAEFCDKDRARLKLAAV
                     MPMHDVRLAVDEARRSVRELGAVALVLSNHPVDGRTWYDTVYDPFWAEAERLGVPVAF
                     HGIQMAYQEHLGRRFMDNFAMAHAAAHPVELMLALGSLLTGGVLERFPGLRAAFLEGS
                     CSWVPWWLWVLDERVEKFGDDERFRLTMPPSEYFKRQCWVSVDPDEHVLQHALPALGD
                     DNIVISSDWPHDDSVYPNAIDTFLGLPGVSEESKRKILWDNCARLYNLGG"
     gene            complement(1293..2381)
                     /locus_tag="VLF19_04800"
     CDS             complement(1293..2381)
                     /locus_tag="VLF19_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HSE92603.1"
                     /translation="MPELFPIGEYWWLYAAFTAGVLVLLGVDLGLLHRDAREVRFREA
                     AGWTAAWVSLALVFNVALYFYARWTFARDERLAAVPGFDPSAAAWQTALEFLTGYVVE
                     YSLSVDNIFVFVLVLGYFTVPPRYQHRVLFYGILGALVFRAAFIALGSVLMQFHWVIW
                     LFGGFLIVTGVKLALSESTIEPERNLLLRLLRRVIPITPGFRGQRFFVREGGRLRGTP
                     LVVALCCLEATDIVFAIDSVPAIYALTREPFVVFTSNVFAILGLRSLYFLLGGAVEKF
                     HLLRYGLAVVLVFVGLKMVWLDELYGGRFPIVASLAIIVSVIAGAILLSLAWPRRPEP
                     AAVAPPGDERARRARARSPETRLGRASR"
     gene            complement(2384..2917)
                     /locus_tag="VLF19_04805"
     CDS             complement(2384..2917)
                     /locus_tag="VLF19_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE92604.1"
                     /translation="MNKVLIATHGYEPQGWADDVVRAVPSPATSLVRVLVVLDTPSAP
                     FTSLIPAARRRYRTALAEWRRLATARTQPSLETLRTGLGRSPEVVHAHARAVDPGRVI
                     VEHARAWGAEVIIIGRDMRSRLSRALLPAAHEHVVNDAPCPVLVTPAERGARAAALVL
                     ERPDLGQRSPAALQGGA"
     gene            complement(3041..3352)
                     /locus_tag="VLF19_04810"
     CDS             complement(3041..3352)
                     /locus_tag="VLF19_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92605.1"
                     /translation="MVVLRWLLILVFSVVLDFVNPALPGALEFFEEAEEMVHRAGHHR
                     IVRLPAASGHPARVGRPPTRVVRTTSRLVAPRRTFEPGSQARKAPPPGIADSPAAPED
                     H"
     gene            3400..4338
                     /locus_tag="VLF19_04815"
     CDS             3400..4338
                     /locus_tag="VLF19_04815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020021187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSE92606.1"
                     /translation="MADVVRQAVQKYRNWGKWGPDDQLGTLNYITPEIVKRSAQMVKR
                     GVTFSLAIPFDSTGPQINQPRRFNPIHRMILTAADFTTGAFTRPGGVGFTDDMVIMAL
                     QCATQWDALAHCFLDGKLYNGYDAHLVSSQGAKKNGIQNMARHIVTRGVLLDIPRVKG
                     VEWLEPGYAITAADLDAAVRAHRVSVGVGDALLIRTGQMTMCRRRGGWGDYAGGDAPG
                     LSFHSAGWLHERQIAAVATDTWGMEVRPNEIPDSYQPLHQVLIPGMGMLVGEIFELDG
                     LAEDCARDGVYEFQLVAPPLPITGAVGSPINPLAIK"
     gene            complement(4812..6023)
                     /locus_tag="VLF19_04820"
     CDS             complement(4812..6023)
                     /locus_tag="VLF19_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HSE92607.1"
                     /translation="MIETLSASEARRLALAAQGFADARPGTRPGWRQMRRTLERLGLL
                     QIDSVNVLVRAHYLPLFSRLGPYDTTALNRAAYSGVRRTVFEYWGHEASLMPVGLHPL
                     FRWRMQRAERGVGIYGGLTRWARRRRPYIDEILAEVAERGPLSASELRNGGKSRGSWW
                     GWSEGKAALEWLFWAGRVTTATRRGFERVYDLPERTLPPAVLAAPTPPEDGAHRALLL
                     RAARALAVATERDLADYFRLGVVDVRRRLAELVEAGALLPARVEGWAQPAFLHPAARA
                     PRRVNARALLSPFDSLVFERQRTERIFGFHYRLSIYTPAPLRAYGYYVLPFLLGDRLV
                     ARVDLKADREARVLRVLAAHAEPEVSPRRVAGALRQELADMAAWLALDRVVVSQRGEL
                     ARALRARSGRS"
     gene            6130..7101
                     /locus_tag="VLF19_04825"
     CDS             6130..7101
                     /locus_tag="VLF19_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92608.1"
                     /translation="MAIQPTETASRLGEYCASLQDAGLDAPWTRPGPLIREKASKVQP
                     CLWRWRQIEPLVRRSPEFMRPGRGAERRIIRLDNPGVPERTSTHTISTAVQYLLPGEV
                     APAHRHTPNAIRFMLHGDGAYTTVEGEKCVMRRGDVVLTPSMTWHDHGNEGREPVMWI
                     DGLDSPVVRYLEDLHMEPYPEERQPVGGVAPRRVFFPWADAHAALRDRAREHGDPFDD
                     VVVEYLDPATGRSLLPTVGCYLQMLRPGVRTRAHRETSSAVYHVVEGRGASVIDGIRL
                     DWGPGDFFAVPPRALHAHAAEGGEPAILFSFQDVPLLTGLGLYRMEA"
     gene            7183..8052
                     /locus_tag="VLF19_04830"
     CDS             7183..8052
                     /locus_tag="VLF19_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSE92609.1"
                     /translation="MRIATWNVNSLRARLEKVTWWLERAKPDVLLMQETKLADADAPD
                     DVFRDLGYELAHHGEGRWNGVAIASRRGIADVVTNFGAPLWPSATPDVADDEPLAEAR
                     MISAVCGGVRVVSLYAPNGRAVDTSFYRAKLAWYEHLARWLAETRDPKEALCLGGDFN
                     VAPADADVWDPAACHGGTHVSEPERLAFGRLREWGLVDAYRLHHAEPGRYTWWDYRAG
                     NFHKNFGMRIDHLLVTAALAERTRWAEIDREARKGKPIPSDHAPLVIDVDEPGCPFDA
                     GWSGAEERIRRRG"
     gene            8049..9137
                     /locus_tag="VLF19_04835"
     CDS             8049..9137
                     /locus_tag="VLF19_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSE92610.1"
                     /translation="MKITHVTTRVLGTPADNPLVVGLPAPTETREFVTLELGTDEGPT
                     GIGLTFFGGALTPALRTAVDTLAQLVVGEDPTRVETIAAKCRRAAGAAGPGGIFTLAL
                     SAVDMACWDLKGKAAGQSVCALLGGLRDRVPTYASGVLMRPHPVDYLAKAGPRLRDMG
                     FRQMKMQCGSEPTAAASIERVRVVREAIGPDVDLMCDINQLWSVNHAIEVGRRLEPYN
                     LFWLEDPTAHDDYPGLARIADALRTPVAAGEYHYGVVPFRHLLEARSIDIVMIDLVRV
                     GGITQWMKVAGMAEAFNLPVVSHLVPEVHAHLIAAIPNGLTVEYMPWTLRLFEETPAL
                     EHGQLVVPKTPGLGLAFDQAAVKKYQVG"
     gene            complement(9271..10140)
                     /locus_tag="VLF19_04840"
     CDS             complement(9271..10140)
                     /locus_tag="VLF19_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92611.1"
                     /translation="MAGETYDRTSQDVGNIVALEHVNVWIPDQQIATLFYVMGLGLTR
                     DPYLMPGLENMWINVGQQQFHLPTNKPQVLRGHVGLVIADFDELPFRLAAVKPRLEGT
                     KFDYSVEDKHIRVVSPWGNQIRCYAPGPQFGEMMHGMPYVEFTVEPGAADGIAAFYRT
                     AMGACATVSRNGATAAHVTVGAHQQLVFRETRETIPAYDGHHIQVYISNFSGPHRWLK
                     ERGLITEESNWYQYRFKDIVDPDTGRALFTIEHEVRSMMSPLYMRPLVNRDPAQRQPT
                     YQRGRDAFVPGMA"
     gene            10296..10805
                     /locus_tag="VLF19_04845"
     CDS             10296..10805
                     /locus_tag="VLF19_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE92612.1"
                     /translation="MGVIVVRDAKDDDLDAAGDVMVAAYAEYLPPDPTGPWLAYREEI
                     GDVRSRLGVATLVVAEDRGRILGAITYFPDACKEAHATWPPAWAVIRLLAVHPDARGR
                     GLGRLLTEACIQRARSTGCRAIGLHTTEFMAVARAMYERMGFTRVPEHDFWPMPQLQV
                     MAYRLDLRD"
     gene            10809..11213
                     /locus_tag="VLF19_04850"
     CDS             10809..11213
                     /locus_tag="VLF19_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92613.1"
                     /translation="MTPYDTTRLPVNRTTVAPDGCDVRVLLALPRGSMAHFSLAPGTT
                     SAAVTHRTVEEIWFFLSGRGEMWRTQAGREEITRVEPGACLTIPLGTHFQLRALGAEP
                     LAAVAVTMPPWPGPAEAEVVPGKWEPTPPRGG"
     gene            11238..>11794
                     /locus_tag="VLF19_04855"
     CDS             11238..>11794
                     /locus_tag="VLF19_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92614.1"
                     /translation="MSRLPRPAPAAAATPRFAALHVRDYRRYYVAGLLAMTADSIEHV
                     ISYWVIFEKFHSPTLAGFAVISHWVPFLLFSVYMGALADRYDCRRLIQAGQALYMVAS
                     IAWGLLFLTDALALWHAVVILLVHGAAGVVVSAAGQLIIHDMVGADHLHSAVRLNASS
                     RNLAILLGPALGGGLMLLLGPAWGLL"
BASE COUNT         1780 a         4244 c         4144 g         1626 t
ORIGIN      
        1 gcaaccgctc gggatcgaag cctcgttctc caacgttcag ccgcccaggt tgtagagccg
       61 cgcgcagttg tcccagagga tcttccgctt gctctcctcg ctgacgccgg gcagcccgag
      121 gaacgtgtcg atggcgttcg ggtagaccga atcgtcgtgg ggccagtcgc tcgagatgac
      181 gatgttgtcg tcgccgaggg cgggcagggc gtgctgcagc acgtgctcgt cgggatccac
      241 cgacacccag cactgacgct tgaagtactc gctgggcggc atggtcagcc ggaagcgctc
      301 gtcgtcaccg aacttctcca cccgctcgtc gagcacccag agccaccaag gcacccagct
      361 gcaggagcct tcgaggaacg cggcccgcag gccgggaaat cgctccagga cgccaccggt
      421 cagcaggctg cccagcgcca gcatcagctc caccgggtgg gcggcggcgt gcgccatcgc
      481 gaagttgtcc atgaaccgcc ggccgaggtg ctcctggtac gccatctgga taccgtggaa
      541 ggccacgggc acgcccaggc gctcggcctc ggcccagaag gggtcgtaga cggtgtcgta
      601 ccacgtgcgg ccgtccaccg gatggttgga cagcacgagc gcgaccgctc ccagctcgcg
      661 cacgctccgg cgggcctcgt cgacggcgag ccgcacgtcg tgcatgggca tgaccgccgc
      721 cagcttgagg cgggcgcggt ccttgtcgca gaactccgcc agccagttgt tgaacgcccg
      781 gcacacggcc gccgacagct cggggtcgag cccgtcgatg gcgacgacgt gcgagcccat
      841 ggtgcggaag acgatcgaga cgtcgatccc ctcgcggtcc atcgcctgga gcatcgcccc
      901 ggggtcgttt cccttgagat cctccttcgg gtcgcggtag cggccagtgg cgacgtgacg
      961 ggcgcgtgcc ttctcccgtc ggatcgacgc caggcgccgg cgctccggcc ggttgatgaa
     1021 cgccgggaag gccatgccct cgaattgcca gccctcgacg tgggaaccct cgatgggctc
     1081 gaaccgcggc gcgcggtcct tgaagcgcgg ctcgatgtag cgcgaccaga cgtcccgcgg
     1141 ctccgtcacg tggatgtcac tgtccatgac cttgaacgat cccttggcca tcgccggcct
     1201 ccatcgtcgt tggttgggcg ccgtcggtcg cgggccccgc ccagggtagc accccgcgcg
     1261 gggacgggac aggccctcga cgggtcgggc gatcatcggc tggcccgccc caggcgcgtc
     1321 tcgggggaac gggcacgggc tcgacgagcc cgctcgtccc cgggtggcgc cacggccgcc
     1381 ggctcgggcc gcctcggcca ggcgagcgac agcaggatcg ctcccgcgat caccgagacg
     1441 atgatggcga gcgacgcgac gatggggaac cggccaccgt acagctcgtc gagccagacc
     1501 atcttgagcc cgacgaagac cagcaccacc gccaggccat agcggagcag gtggaacttc
     1561 tcgacggccc cgccgagcag gaagtacagc gagcgcaggc ccaggatcgc gaacacgttc
     1621 gacgtgaaca cgacgaacgg ctcacgggtc agggcgtaga tggccgggac cgagtcgatg
     1681 gcgaagacga tgtcggtggc ctccaggcag cagagggcga cgacgagcgg cgtgccccgg
     1741 agccggccgc cctcgcgcac gaagaaccgc tgaccacgaa accccggcgt gatggggatc
     1801 acgcgacgca gcagccgcag gagcaggttg cgctccggct cgatggtgga ttccgacagt
     1861 gcgagcttca ccccggtgac gatcaggaac ccaccgaaga gccagatcac ccagtggaac
     1921 tgcatcaaca cggatcccag ggcgatgaac gccgcgcgga agacgagggc gccgaggatg
     1981 ccgtagaaca gcacgcgatg ctggtagcgc ggcggcaccg tgaagtatcc gaggacgagg
     2041 acgaacacga agatgttgtc caccgatagc gagtactcga cgacataccc ggtgaggaac
     2101 tccagcgcgg tctgccaggc ggccgcgctc ggatcgaagc ccggcaccgc ggccaggcgt
     2161 tcgtcccggg cgaacgtcca gcgcgcgtag aagtacagcg cgacgttgaa gacgagggcc
     2221 aggctcaccc aggccgccgt ccatccggcg gcctcgcgga agcggacctc gcgggcgtcg
     2281 cggtggagga ggccgaggtc cacccccagc aagacgagga ccccggccgt gaaggccgcg
     2341 tacagccacc agtattcccc gatcggaaag agttcgggca tggtcacgcg cctccctgga
     2401 gcgcggcggg tgaccgctgg ccgaggtcgg ggcgctcgag cacgagcgcg gcggcgcggg
     2461 caccccgctc ggccggcgtg acgaggacgg ggcacggcgc gtcgttcacc acgtgctcgt
     2521 gcgcggccgg gaggagcgcc cgcgacagcc ggcttcgcat gtcgcggccg atgatgatta
     2581 cctcggcgcc ccacgcccgg gcgtgctcga cgatgacgcg cccgggatcc accgctcgcg
     2641 cgtgcgcgtg gaccacttcc ggtgatcgcc cgaggccggt ccgcaacgtc tcgagcgaag
     2701 gctgcgtccg tgctgtcgcg aggcgacgcc actcggcgag cgcggtccgg tagcgccggc
     2761 gggccgcggg gatgagcgac gtgaatggcg ctgacggcgt gtcgagcacc accagcaccc
     2821 gcaccaggct cgtggccggc gacggcaccg cccggacgac gtcgtccgcc cacccctgcg
     2881 gctcgtatcc atgcgtggcg atgagaacct tgttcatggc gacctccccg tcggttgtcg
     2941 gcttcgtgat gcgccggcgc gaagacgcgc agtgctgcgc acgggcgcgc aggccacgat
     3001 cagcgcggcg cgggggaacg gagcggaggg gacggggagg ctagtggtct tcgggagcgg
     3061 ccggagagtc agcgataccc ggaggcggcg ctttccgcgc ctggctgcca ggctcgaacg
     3121 tccgccgagg cgcgacgagc cgggacgtcg tccggacgac ccgcgtgggc ggtcgaccga
     3181 cgcgggctgg atgaccgctg gcggccggca gtcggacgat ccggtggtgg cccgcgcggt
     3241 ggaccatctc ctcggcttcc tcgaagaact ccagggcgcc gggcagcgcc ggattcacga
     3301 agtcgaggac gaccgagaac accaggatca ggagccagcg caggacgacc atacgagggt
     3361 atgctaccac gcactctgtg acgccaacgg gagacagcca tggccgacgt cgtcaggcag
     3421 gcggtccaga agtatcgcaa ctggggcaag tgggggccgg acgaccagct cggcacgctg
     3481 aactacatca cccccgagat cgtgaagcgc tccgcccaga tggtcaagcg cggcgtgacg
     3541 ttctcgctgg cgattccgtt cgacagcacc ggtccccaga tcaaccagcc gcggcgcttc
     3601 aacccgatcc accgcatgat cctcaccgcc gcggacttca cgaccggcgc cttcacgcgt
     3661 cctggcggcg tcgggttcac cgacgacatg gtgatcatgg ccctgcagtg cgcgacccag
     3721 tgggacgccc tcgcccactg tttcctggac ggcaagctct acaacggcta cgacgcgcac
     3781 ctcgtgtcga gtcagggcgc caagaagaac ggcatccaga acatggcgcg ccacatcgtc
     3841 acccgtggcg tgctcctgga catcccgcgc gtcaagggcg tggagtggct ggagccgggc
     3901 tacgccatca ccgccgcgga tctcgacgct gccgtccgcg cccatcgcgt gagcgtgggc
     3961 gtcggcgacg cgctcctgat tcgcaccggg cagatgacca tgtgccgccg caggggcggc
     4021 tggggcgact acgcgggcgg cgacgcgccg ggcctgtcgt tccacagcgc cggatggctg
     4081 cacgagcgcc agatcgccgc cgtggccacc gacacctggg gcatggaggt gcggcccaac
     4141 gagatccccg acagctacca gccgctccac caggtgctca tcccgggcat gggcatgctc
     4201 gtcggcgaga tcttcgagct cgacggcctc gccgaggact gcgcgcgcga cggcgtctac
     4261 gagttccagc tcgtggcgcc gcccctgccc atcacgggcg ccgtcggctc gccgatcaat
     4321 ccgctcgcga tcaagtgacg ggggccgccg ccccggatcg ccgcggagcc gccagcgtcc
     4381 ggcgggcttg accagcgccg cgaagcgctc tatccgctcc aggacctccg cgaggcggtc
     4441 gctgcggaag tcgacgacca gctcgctcac gccgagccgc gcgtaggtgt ccacgtcctc
     4501 caggatctgg tccggcgcgc cgctgaaggg gcgccgcccg ccggcgtcgg tcacctcgac
     4561 gtcgtacacc ggcgccttga aggagatcgc gaggcgcgat ggatcgcgtc cctccttctc
     4621 ggtcaggcgg tggagctcgt cgagcaggcc ccgcatctcc ggcggccgga gcagcaccgc
     4681 ggcgttggcg ccgaccgggt gccagccgtc gccgtgacgg gcgacgcgcg catgatcgat
     4741 cctccttcgg gtcgggcccc tcaggccccg gacgccgttg gtcccctcca cgaccagggc
     4801 cggggaggcg atcaggaacg gcctgatcga gcgcgtagcg cgcgggcgag ctcgccgcgc
     4861 tgggagacga cgacgcggtc gagcgcgagc cacgccgcca tgtcggcgag ctcctgccgg
     4921 agcgctccgg ccacccgacg cgggctcact tcgggttcgg cgtgcgccgc gagaacacgg
     4981 agcacgcgcg cctcgcggtc ggccttgaga tcgacgcgcg cgaccagacg gtcgccgagc
     5041 agaaacggca agacgtagta cccgtacgcc cgcaggggcg cgggcgtgta gatcgacagg
     5101 cgatagtgga agccgaagat ccgctcggtc cgctggcgct cgaagaccag ggagtcgaag
     5161 ggcgacagca gggcgcgcgc gttcacgcga cgtggcgccc gcgccgcggg atggagaaag
     5221 gccggctgcg cccagccctc gacccgggcc ggcagcagcg ccccggcctc gaccagctcg
     5281 gcgagccggc gccgcacgtc gacgacaccg agacggaagt aatcggccag atcgcgctcg
     5341 gtcgccaccg ccagcgcgcg ggccgcgcgc aggagcaggg cacggtgggc cccgtcctcg
     5401 gggggcgtcg gcgcggcgag gaccgccggc ggcagggtgc gctcgggcag gtcgtagacg
     5461 cgctcgaatc cgcgacgagt cgccgtcgtc acccggcccg cccagaagag ccattccagc
     5521 gccgccttgc cctcgctcca gccccaccag gagccgcggc tcttgcctcc gttccggagc
     5581 tcggaggccg agaggggtcc ccgctccgcc acctctgcca ggatctcgtc gatgtagggc
     5641 cgccgccgac gggcccagcg cgtgaggccg ccgtagatac cgacgccccg ctcggctcgc
     5701 tgcatccgcc agcgaaagag cggatgcagc cccaccggca tgagcgacgc ctcgtgcccc
     5761 cagtactcga agacggtgcg tcgcacgccg ctgtacgcgg cgcggttcag cgctgtcgtg
     5821 tcgtagggcc ccaggcgcga gaacagcggc agatagtgcg cgcggaccag gacgttcacc
     5881 gagtcgatct gcaggagccc gaggcgctcc agcgtgcggc gcatctgacg ccagcccggg
     5941 cgcgtgccgg gacgtgcatc agcgaacccc tgggcggcca gggcgaggcg acgcgcttcg
     6001 gaggcggaga gcgtctcgat caatgggatc cggcgcgggg cccgtggcct cgttggtgca
     6061 atcgtcagcg tcttgtacca taattccgcc cgcaacgtca tcgaccggag cacccccggg
     6121 aggagcacga tggcgatcca gccgacggag acggcgagcc gcctcggtga gtactgtgcg
     6181 agcctccagg acgccggtct ggacgcgccg tggacgcggc ccggcccgct gatccgggag
     6241 aaagcctcga aggtccagcc gtgtctctgg cgctggcggc agatcgagcc gctcgtccgg
     6301 cgcagccccg agttcatgag gccgggccgg ggcgccgagc ggcgcatcat ccggctcgac
     6361 aaccccggtg tgcccgagcg gacgagcacc cacacgatct cgacggccgt gcagtacctg
     6421 ctcccgggag aggtcgcccc ggctcaccgg cacacgccga acgccatccg cttcatgctg
     6481 cacggcgatg gcgcctacac gaccgtcgag ggcgagaaat gcgtcatgcg gcggggcgac
     6541 gtcgtgctca cgccgtcgat gacctggcac gaccacggca acgagggtcg cgagcccgtg
     6601 atgtggatcg acggcctcga ctcgcccgtg gtccgctacc tcgaggacct ccacatggag
     6661 ccctacccgg aggagcggca gcccgtcggc ggcgtggcgc cgcgccgcgt gttcttcccg
     6721 tgggccgacg cccacgccgc gctccgcgac cgggcgcgcg agcacggcga ccccttcgac
     6781 gacgtggtcg tggagtacct cgacccggct accggacgct cgctgcttcc cacggtcggg
     6841 tgctatctcc agatgctgcg tcccggcgtg agaacccgcg cccatcgcga gacctcgagc
     6901 gcggtctatc acgtcgtcga gggccgcggc gcgagcgtga tcgacggtat ccgcctcgac
     6961 tgggggcccg gcgacttctt cgcggtgccg ccccgcgcgc tccacgccca cgccgccgag
     7021 ggcggtgagc cggcgatcct gttctcgttc caggacgtgc cactgctcac cgggctcggc
     7081 ctctaccgga tggaggcgtg agcgcatcgc cattcccgcc ggtgatcccg tagtaagctg
     7141 ggctgccgcg ccggcggcgc acgcccggca ccggggagcc gaatgcggat cgccacctgg
     7201 aacgtcaact cgctcagggc ccggctcgag aaggtcacgt ggtggctcga gcgggcgaag
     7261 cccgacgtgc tcctgatgca agagacgaag ctcgccgacg ccgacgcccc ggacgacgtg
     7321 ttccgcgacc tcggttacga gctggcccac cacggcgagg ggcgctggaa cggcgtggcc
     7381 atcgcgagcc gccgcggcat cgctgacgtg gtgaccaact tcggcgcgcc gctgtggccg
     7441 tcggccacgc cggacgtcgc cgacgacgag ccgctggccg aagcgcggat gatctcggcc
     7501 gtgtgcggcg gtgtccgcgt cgtgagtctc tacgcgccga acggccgcgc ggtcgacacc
     7561 tccttctatc gggcgaagct cgcgtggtac gagcacctgg cgcgctggct cgccgagacg
     7621 cgcgacccga aggaggccct ctgtctcggc ggcgacttca acgtggcccc ggccgacgcc
     7681 gacgtctggg accccgcggc gtgtcacggc gggacgcacg tctccgagcc ggagcgcctg
     7741 gccttcggcc ggctccgcga gtggggcctc gtcgacgcct accgcctgca ccacgccgag
     7801 cctgggcgct acacgtggtg ggactatcgt gccggcaatt tccacaagaa cttcggcatg
     7861 cggatcgacc acctgctcgt caccgccgcg ctggcggagc gcacccgctg ggcggagatc
     7921 gaccgcgagg cccgcaaggg caaacccatc ccctcggacc atgcgccgct cgtgatcgac
     7981 gtcgacgagc cgggctgccc gttcgacgcc ggctggagcg gcgcggagga gcgcatcagg
     8041 agacgcggat gaagatcaca cacgtcacca cgcgcgtgct cggcaccccc gccgacaacc
     8101 ccctggtcgt cgggctgccc gcgcccaccg agacgcgcga attcgtgacg ctcgagctgg
     8161 gcaccgacga gggcccgacc ggcatcgggc tcacgttctt cggcggggcc ttgacccccg
     8221 cgctccggac cgcggtggac acgctggcgc agctcgtcgt cggcgaggac cccacccggg
     8281 tggagacgat cgcggcgaag tgccgccgcg ccgccggcgc tgcgggcccg ggcggcatct
     8341 tcacgctcgc gctctcggcc gtcgacatgg cctgctggga cctcaagggc aaggccgccg
     8401 gccagtccgt ctgcgcgctc ctcggcgggc tgcgcgaccg cgtgccgacc tacgcgagcg
     8461 gcgtcctcat gcgcccgcac ccggtggact acctggcgaa ggccggccct cgcctccgcg
     8521 acatgggctt ccgccagatg aagatgcagt gcgggagcga gccgaccgcg gcggcctcga
     8581 tcgagcgcgt gcgcgtcgtg cgggaggcca tcggccccga cgtcgacctg atgtgcgaca
     8641 tcaaccagct gtggtccgtg aaccacgcca tcgaggtcgg gcggcgcctc gagccctaca
     8701 atctcttctg gctcgaggac cccaccgccc acgacgacta tcccggactc gcgcgcatcg
     8761 ccgacgcgct ccgcaccccc gtcgccgccg gtgagtatca ctacggcgtc gtgcccttcc
     8821 gccacctgct cgaggcacgg tcgatcgaca tcgtgatgat cgacctcgtg cgtgtgggcg
     8881 gcatcacgca gtggatgaag gttgccggca tggccgaggc cttcaacctg ccggtggtga
     8941 gccacctcgt gccggaggtc cacgcccacc tcatcgccgc catccccaac ggcctcaccg
     9001 tggagtacat gccgtggacg ctccggctct tcgaggagac gccggcgctc gagcacggcc
     9061 agctcgtggt gcccaagacg ccggggctgg ggctcgcgtt cgatcaggcc gcggtcaaga
     9121 agtatcaggt ggggtgacgt cgggtcgatc gccgccgccg actcttgggc agacccgcgc
     9181 ccgagacacc gcatgtcgag cgccggcttt gccggcgcaa tcctgctctg ggggaggcct
     9241 cggaggggac cgtcgaggcc ccctccgatt tcacgccatg cccggcacga aggcgtcgcg
     9301 cccgcgctgg taggtcggct gccgctgtgc cgggtcgcgg ttgaccagcg gccgcatgta
     9361 gagcgggctc atcatgctcc gcacctcgtg ctcgatcgtg aagagcgccc ggcccgtgtc
     9421 ggggtcgacg atgtccttga agcggtactg gtaccagttg ctctcctcgg tgatcagccc
     9481 gcgctccttg agccaccggt gcggacccga gaagttcgag atgtagacct ggatgtggtg
     9541 cccgtcgtag gccgggatcg tctcgcgagt ctcccggaag acgagctgct ggtgggcccc
     9601 caccgtcacg tgggccgccg tcgcgccgtt gcggcttacc gtcgcgcacg cgcccatcgc
     9661 cgtccggtag aacgccgcga tgccgtccgc cgccccgggc tcgacggtga actccacgta
     9721 tggcatgccg tgcatcatct cgccgaactg gggcccgggc gcgtagcagc ggatctggtt
     9781 gccccacggc gacaccaccc ggatgtgctt gtcctccacc gagtagtcga acttcgtccc
     9841 ctcgagcctc ggtttgaccg cggccaggcg gaagggtagc tcgtcgaagt cggcgatcac
     9901 caggcccacg tggccgcgca gcacctgggg cttgttcgtg ggcaggtgga actgctgctg
     9961 cccgacgttg atccacatgt tctctaggcc gggcatcagg tagggatcac gggtcaaccc
    10021 gagccccatg acgtagaaga gggtggcgat ctgctgatcc ggaatccaca cattcacgtg
    10081 ctcgagcgcc acgatgttgc cgacgtcctg gctcgtccgg tcgtacgtct cgcccgccat
    10141 ggtcgcctcc ctcacagtcg gtgccgctgc cggccctagg gtaacgcgac gccaatgtcc
    10201 agacaacacg ggctcatcgg agacttcacc ggggccggcg cggcgccttc tccaggtcgc
    10261 acccttgacg gggcggtacc ggccgcggta aggcgatggg cgtgatcgtc gtgcgcgacg
    10321 ccaaggacga cgacctcgac gcggccggcg acgtgatggt cgcggcgtac gccgagtacc
    10381 tgccgcccga cccgacgggg ccctggctcg cctaccgtga ggagatcggc gatgtgcgga
    10441 gccgcctcgg cgtggcgacg ctcgtcgtgg ccgaggaccg cggccgaatc ctcggggcca
    10501 tcacgtactt ccccgacgct tgcaaggaag cgcacgccac ctggccgccg gcatgggcgg
    10561 tcatccggct gctcgccgtc caccccgatg ctcgcgggcg cggcctggga cggctcctga
    10621 ccgaggcctg catccagcgc gcgcgcagca ccgggtgccg ggccatcggc ctgcacacga
    10681 cggagttcat ggccgtcgct cgcgcgatgt acgagcgcat gggcttcacg cgcgtgccgg
    10741 agcacgattt ctggccgatg ccccagctcc aggtcatggc gtaccggctc gatctccggg
    10801 actgaccggt gacgccctac gacacgacgc ggctacccgt gaaccgcacg acggtggcgc
    10861 ccgacggatg cgacgtgcgc gtcctgctcg ccctgccgcg cggtagcatg gcgcacttct
    10921 cgctcgcgcc ggggacgacg tcggcggcgg tgacgcatcg gacggtcgag gagatctggt
    10981 tcttcctctc gggccgcggc gagatgtggc gcacgcaggc cgggcgggaa gagatcaccc
    11041 gggtcgaacc gggcgcgtgc ctgacgatcc ccctcggcac tcacttccag ctccgcgcgc
    11101 tgggcgccga gccgctcgcg gccgtcgcgg tcaccatgcc tccgtggccg ggcccggcgg
    11161 aagccgaggt cgtcccgggg aagtgggaac ccacgccgcc ccgaggagga tgacgcggcc
    11221 gatggtacga tgcggcggtg agccgcctgc cccgcccggc gccggccgcg gcggccacgc
    11281 cgcggttcgc agccctccac gtgcgcgact accgccggta ctacgtcgcc ggcctgctgg
    11341 ccatgaccgc cgacagcatc gagcacgtca tcagctactg ggtcatcttc gagaagttcc
    11401 actcgcccac gctggcggga ttcgccgtca tcagccactg ggtgccattc ttgctcttct
    11461 cggtgtacat gggggcgctc gccgaccgct acgactgccg caggctcatc caggccggcc
    11521 aggcgctcta catggtcgcc tcgatcgcgt ggggactgct gttcctcacc gacgcgctcg
    11581 cgctctggca cgccgtcgtc atcctgctcg tccacggcgc tgccggtgtc gtcgtgagcg
    11641 ccgccggcca gctcatcatc cacgacatgg tgggcgccga tcacctgcac agcgcggtcc
    11701 gcctgaacgc cagcagccgc aacctcgcca tcctgctcgg gccggcgctc ggcggcggtc
    11761 tgatgctcct gctcgggccg gcgtggggcc tgct
//
LOCUS       DATIAZ010000138         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_560944, whole genome shotgun sequence.
ACCESSION   DATIAZ010000138 DATIAZ010000000
VERSION     DATIAZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560944"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VLF19_04860"
     CDS             complement(<1..333)
                     /locus_tag="VLF19_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSE92615.1"
                     /translation="MRNGRQHTTEPTRAPRERALIAALRLPGQRRWEVEESLDELAGL
                     AAAAGATVVHRVVQERRAPAPALHFGRGKIEEIGQAARATRANLVITDDALTPVQERN
                     MSQTLGLKV"
     gene            complement(330..1268)
                     /gene="miaA"
                     /locus_tag="VLF19_04865"
     CDS             complement(330..1268)
                     /gene="miaA"
                     /locus_tag="VLF19_04865"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSE92616.1"
                     /translation="MSVPLLVIAGPTGVGKTAAAAALARRIPLEVVSADSRQVYRGMD
                     VATGKPTAAERRAVTHHLIDVVDPGDRYHAARFRNDASVLIADIRARGRLAAVVGGTG
                     LYIRALLRGLDPAPPADPALRGELAEVVVRDGRAALHARLEREAPALARRLHPNDHVR
                     VVRALERIRAQGAPAMHQERWARPAAEYTALYVGLTMERARLARRLRERAATMVEAGL
                     TDEVARLLARGYDPSLPAMQGIGYREFVQVVHGRLGREEALRLMQRDTVRYAKRQWTW
                     FSREPDVQWLDVDLAGGSETVAAIIEQRLHERGFIE"
     gene            complement(1311..>2211)
                     /locus_tag="VLF19_04870"
     CDS             complement(1311..>2211)
                     /locus_tag="VLF19_04870"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92617.1"
                     /translation="RPLLARDRFPLVALDVRLPLADVDVNVHPTKAWVRFRHPRLVQE
                     MVVAALGEALRRRAMAPGVDTGGHAPGVAAPRAGETAPPDGGGPAAFGPGLAGQALFE
                     ATAPYVAADLGRVLGQVQDTFVVAATDNEVFFVDQHAAHERVVFERLQADLAAGAPAA
                     QALLFPEPLDLAPAARALLERWREPLERLGFAFEDAGGRSVVLRAVPAVLQRDEPRRL
                     VEAAVDELAAARPGELVIDRVLSFVACRAAIKANTPLEREEMERLVAELGATAAPYFC
                     PHGRPTMSRVSIQDIRRDVRRAW"
BASE COUNT          288 a          842 c          804 g          277 t
ORIGIN      
        1 gaccttcagc ccgagcgtct gcgacatgtt ccgctcctgg acgggcgtca gcgcgtcgtc
       61 ggtgatgacg aggttcgcgc gggtcgcgcg cgccgcctgg ccgatctcct cgatcttgcc
      121 gcggccgaag tgcagcgcgg gtgccggcgc gcgtcgctcc tgcacgaccc gatggacgac
      181 ggtggcgccg gcggcggcgg cgaggcccgc cagctcgtcg agcgattcct cgacctccca
      241 gcggcgctgt ccgggcaggc gcagggccgc gatcagggcg cgctcgcgtg gcgcgcgggt
      301 cggctcggtg gtgtgctgac ggccattcct cactcgatga atcccctttc gtggagtcgc
      361 tgctcgatga tggccgcgac ggtctcggac ccgccggcga ggtccacgtc gagccactgc
      421 acgtccggct cccggctgaa ccatgtccac tgccgcttcg cgtaccgtac ggtgtcccgc
      481 tgcatgagcc tcagcgcctc ctcgcggccg aggcggccgt ggaccacctg gacgaactcg
      541 cggtacccga tgccctgcat ggccggcagc gagggatcgt agccgcgcgc cagcaggcgc
      601 gccacctcgt cggtcaggcc ggcctccacc atggtcgccg cgcgctcgcg caggcgccgc
      661 gccagacggg cgcgctccat cgtgaggccg acgtagaggg cggtgtactc ggctgccggc
      721 cgcgcccagc gctcctggtg catcgccggc gcgccctggg cccggatccg ctcgagcgcc
      781 cgaacgaccc gcacgtgatc gttcggatgc aggcgccgcg ccagcgcggg agcctcgcgc
      841 tccagacggg cgtgcagggc cgcccggccg tcgcggacca cgacctcggc cagctcacct
      901 cgcagcgcgg gatcggccgg aggcgcggga tcgagcccgc ggagcagggc gcggatgtac
      961 aggccggtgc cgccgaccac cgccgccagc cgcccgcgcg cccggatgtc ggcaatgagc
     1021 acgctcgcgt cgttccgaaa gcgcgccgcg tgatagcggt cgcccgggtc caccacgtcg
     1081 atcaggtgat gcgtgacggc gcggcgctcc gcggccgtcg gcttgccggt ggccacgtcc
     1141 ataccgcggt aaacctgacg cgagtcggcg ctgacgacct ccagcggaat gcggcgggcc
     1201 agggcggcgg cggccgcggt cttgccgacg cccgtgggac ccgcgatgac caggagggga
     1261 acgctcatcc gttacccgca gatcctagcg cggcccacct cgccctgcca ctaccacgcc
     1321 ctccggacat cccggcggat gtcctggatc gagacgcggc tcatggtggg ccggccgtgc
     1381 ggacagaagt agggtgccgc ggtggcgccg agctccgcga ccagacgctc catctcctcg
     1441 cgttcgagcg gcgtgttcgc cttgatcgcg gcccggcagg cgacgaacga caggacgcga
     1501 tcgatgacga gctcgcccgg cctggccgcc gccagctcgt ccaccgcggc ctccaccagg
     1561 cgccggggct cgtcgcgctg cagcacggcc ggcacggcgc gcaggaccac cgaccgaccg
     1621 cctgcgtcct cgaatgcgaa tcccagacgt tcgagcggct ccctccagcg ctcgagcagc
     1681 gcccgcgccg ccggggcgag atcgaggggc tccgggaaca gcagcgcctg cgcggccgga
     1741 gcgccggccg cgagatcggc ctgtagccgc tcgaagacga cgcgctcgtg ggcggcgtgc
     1801 tgatccacga agaagacctc gttgtcggtt gcggcgacga cgaaggtgtc ctgcacctgg
     1861 cccagcacgc gcccgagatc cgcggccacg tagggcgccg tcgcctcgaa gagcgcctgc
     1921 cctgccagcc ccggtccgaa cgctgccgga ccgccgccgt ccggtggcgc cgtctcgccc
     1981 gcgcgcggcg ccgccacgcc cggggcgtgg ccgccggtat cgacgcccgg tgccatggcg
     2041 cgccggcgca gcgcctcacc cagcgcggcc acgaccattt cctgcacgag ccgggggtgc
     2101 cggaagcgga cccacgcctt cgtcgggtga acgttcacgt cgacgtcggc gagcggcagg
     2161 cgaacgtcca gggcgacgag ggggaaccga tcgcgcgcca gcagcggccg g
//
LOCUS       DATIAZ010000139         5054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_691765, whole genome shotgun sequence.
ACCESSION   DATIAZ010000139 DATIAZ010000000
VERSION     DATIAZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691765"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VLF19_04875"
     CDS             <1..587
                     /locus_tag="VLF19_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92618.1"
                     /translation="RVFEPFFTTKPGATGLGLCLAHGIVSRHGGQIEVDSAPGRGTTV
                     RIRFPVEVVAREKPAAPPRAATLPGGPARCLVVDDDPQVREMIRDILSNVGHAVVVAV
                     DGADGVEKFKADPSFDLVVTDLAMPKLNGLQLARVCKTLRPAVPVIMLTGWGVLLTET
                     ELAEHGVDEVLSKPVRMDQVLGSVAAVRGRAARA"
     gene            597..1667
                     /gene="trpD"
                     /locus_tag="VLF19_04880"
     CDS             597..1667
                     /gene="trpD"
                     /locus_tag="VLF19_04880"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSE92619.1"
                     /translation="MASPIITEAVRALVDRRDLSRIEAAAAMEAIMSGAASDAQIAAF
                     LTALRMKGETVEELIGFAQVMRQKAVRVRARGEDVAALTGTDREMLIDTCGTGGDAAG
                     TFNVSTAVAFVVAGAGLRVAKHGNRSVSSLCGSADVVETLGISLELPPSKVARCIDEV
                     GIGFLYAPLLHTAMKHVMAARREMGIRTVFNMLGPLTNPAGANAQLIGVYAAALTEPL
                     ARVLAELGTIRAFVVHGADGLDEISNTGESRLSEVREGLVRTFTLRPEDFGMAQAAIA
                     ELRGGDREQNAQIIRALLDGEAGPRRDIVLMNAAAALVAGAKVRDFKAGVELAARAID
                     SGAARGRLQALAAFSHKLAREA"
     gene            2032..3150
                     /locus_tag="VLF19_04885"
     CDS             2032..3150
                     /locus_tag="VLF19_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSE92620.1"
                     /translation="MRVETLAIHAGRAVDSATGAVALPIHPSTTFERDADGGYARGHL
                     YSRNSNPNRDALERCLADLEGGAAAAAFASGNAASAAILLSLAPGDHVVAPVDLYYGT
                     ARLMREHMARWGLEVTFVDMRDPAEFAAALRPSTRLVWLETPSNPMLTVTDVAAVAAP
                     ARRAGVVVVCDNTLATPVLQRPLALGADLVKHSTTKFFGGHADVLGGAVVAARADGFF
                     EKVRALQAATGGVPAPFDCWLLLRSIRTLPWRVRAHADNALAVARFLRAHPRVEAVHY
                     PGLPDHPAHAVAARQMSAFGGVLSFEVRGGRDAAMAVAAKLRLITRATSYGSVESLVE
                     HRASIEGPQSRTPQGLLRLSVGLEHPDDVIEDLAQALG"
     gene            complement(3178..4152)
                     /locus_tag="VLF19_04890"
     CDS             complement(3178..4152)
                     /locus_tag="VLF19_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HSE92621.1"
                     /translation="MKRVAVLDDYQGAAAALPYWTRLEGRARLDVFRDTLKNEAALAQ
                     RLAPYEIIVPIRERTRFPASLLERLPRLELLALTGRNSGHVDVLAATSRRVLVTETAG
                     SAAAAVEHTMALLLALVRRIPQEDRALREGRWQTSVGTELAGKTLGVVGMGRIGGHIA
                     AFGTFLGMKVLAWGPTLTAERAAAGGARFVPLETLLRESDVVSINTRLSGKTRGLITA
                     RELAMMKSTAYLVNTARGPIVDEAALVAALRGRRIAGAALDVFDDEPLPASHPLLALD
                     NVVLTPHIGYVTREAYDAFFGHAVEAIVAYLDGKTPPRALNPDVLHPR"
     gene            complement(4155..4481)
                     /locus_tag="VLF19_04895"
     CDS             complement(4155..4481)
                     /locus_tag="VLF19_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92622.1"
                     /translation="MPAVVGAPQVRATIQGVRVDVHRQAGGDRDTIYSIRLLGSGNQP
                     LTGAEVSLLGQAANGVPLSAPLIPASEPGLYRARLASESPRGLRLRVVHARARFEVSL
                     DHAVSW"
BASE COUNT          669 a         1826 c         1894 g          665 t
ORIGIN      
        1 cccgcgtctt cgagccgttc ttcacgacca agccgggcgc gaccggactc ggcctctgcc
       61 tggcccacgg catcgtgtcg cgccacggcg ggcagatcga ggtggactcc gcgccgggac
      121 gcgggacgac ggtgcggatc aggttcccgg tggaggtcgt cgcccgggaa aagccggcgg
      181 cgccgccccg cgcggccacc ctccccggcg ggcccgcgcg gtgcctcgtc gtggacgacg
      241 atccccaggt gcgggagatg attcgcgaca tcctctcgaa cgtcggacac gccgtcgtgg
      301 tcgccgtgga cggcgcggat ggcgtcgaga agttcaaggc cgatccgtcg ttcgacctcg
      361 tggtcacgga cctcgccatg ccgaagctca acgggctgca gctcgcgcgc gtctgcaaga
      421 ccctccggcc ggcggtgccg gtcatcatgc tcacgggctg gggcgtgctg ctcacggaga
      481 ccgagctggc cgagcacggg gtggacgagg tgctctccaa gccggtgcgc atggaccagg
      541 tgctcggcag cgtcgcggcc gtgcgcgggc gtgcggccag ggcctgaggc gtcggcgtgg
      601 cgagcccgat catcaccgag gccgtccggg ccctggtcga ccggcgggat ctcagccgca
      661 tcgaggccgc ggcggccatg gaagccatca tgtcgggcgc cgccagcgac gcgcagatcg
      721 cggcgttcct gaccgcgctc cgcatgaagg gcgagaccgt cgaggagctg atcggcttcg
      781 cccaggtgat gcgccagaag gccgtgcgcg tgcgggcgcg cggcgaggac gtggcggcgc
      841 tcacgggcac cgaccgggag atgctcatcg acacgtgcgg caccggcggt gacgccgccg
      901 gcaccttcaa cgtctccacg gccgtggcgt tcgtcgtcgc cggcgccggt ctcagggtcg
      961 ccaagcacgg caaccgctcg gtgtcctcgc tctgcggctc ggccgacgtc gtggagacgc
     1021 tcggcatcag cctcgagctg ccgccgtcca aggttgcccg gtgcatcgac gaggtcggca
     1081 tcggcttcct ctacgcgccg ctcctgcaca ccgcgatgaa gcacgtgatg gcggcgcggc
     1141 gcgagatggg catccgcacc gtcttcaaca tgctcgggcc cctgacgaac ccggccgggg
     1201 ccaacgcgca gctcatcggt gtctacgccg ccgccctcac cgagccactc gcccgcgtcc
     1261 tcgccgagct cggcacgatc cgggccttcg tcgtgcacgg ggccgacggc ctcgacgaga
     1321 tctccaatac cggcgagagc cggctgagcg aggtgcgcga gggcctcgtg cggaccttca
     1381 cgctgcggcc cgaggacttc gggatggcgc aggcggccat cgccgagctc cggggcggcg
     1441 accgcgagca gaacgcgcag atcatccgcg cgctgctgga cggtgaggcc ggccccaggc
     1501 gggacatcgt gctcatgaat gccgcggcgg cgctcgtggc cggtgcgaag gtgcgcgact
     1561 tcaaggcggg cgtggagctg gcggcgcgcg ccatcgacag cggcgccgcg cgcggccggt
     1621 tgcaggcgct cgccgccttc agtcataagc tcgcccgaga agcctgactc cttcccgggg
     1681 aacgaccagc gccccgcgga gcccgccctc cgccctgaac tcgtgtcgga atgcgcggcg
     1741 ggaccggcaa gggagcgagg gaccgtcccc cgggacggcg gagaaaagag aaccgcgccc
     1801 acaggggcat atgggcgcgg ctcccggagg cgcccggcgg tggcctgccc gcgtgcatgc
     1861 ggcccggctc cgccgtcaac cgcccccgaa cccggagatg aagcaagagc cgtacccgtg
     1921 ctcctgcgcc cgcatggtcg gcaaacaccg cgccggaatg gctgttgccc atcgcatgct
     1981 gcagcggatt accattcctc cgacaaagag tgcgcggcag gtggaggact catgagagtc
     2041 gagacgctgg caattcacgc agggagggcc gtcgactcgg cgaccggagc cgtggcgctg
     2101 ccgatccatc cctcgaccac cttcgagcgc gacgcggacg gcggctacgc gcggggtcac
     2161 ctctactcac gcaacagcaa tccgaaccgc gacgccctcg agcgctgcct ggccgacctg
     2221 gagggggggg cggccgcggc ggccttcgcg tccggcaacg cggccagtgc ggcgatcctc
     2281 ctgtcgctcg cgcccggcga tcacgtcgtc gcccccgtcg acctgtatta cgggacggcc
     2341 aggctgatgc gcgagcacat ggcgcgctgg gggctggagg tcaccttcgt ggacatgcgc
     2401 gacccggccg agttcgcggc ggcgctccgc ccgagcacgc ggctcgtgtg gctggagacg
     2461 ccttccaatc ccatgctgac ggtcacggac gtcgcggccg tcgcggcacc ggcgcgccgc
     2521 gccggcgtcg tcgtcgtctg cgacaacacg ctggcgacgc ccgtgctcca gcggccactg
     2581 gcgctcggcg cggacctcgt gaagcactcg acgacgaagt tcttcggggg tcacgccgac
     2641 gtgctcggcg gtgccgtggt ggcggcccgg gccgacggct tcttcgagaa ggtccgtgcg
     2701 ctgcaggcgg cgaccggcgg cgtgcccgcg cctttcgact gctggctgct gctgcgctcg
     2761 atccggacgc tgccctggcg ggtccgcgcg cacgccgaca atgccctcgc ggtcgcgcgc
     2821 ttcctgcgcg cccacccgcg ggtcgaggcc gtgcactacc ccgggctgcc cgatcatccg
     2881 gcccatgccg tggccgcccg gcagatgagc gcgttcggcg gcgtcctgtc cttcgaggtc
     2941 cgcggcggcc gggacgcggc gatggccgtc gccgccaagc tgcgtctgat cacgcgcgcc
     3001 acgagctacg gcagcgtgga gagcctcgtc gagcaccggg cgtcgatcga gggaccgcag
     3061 agccgcacgc cgcaagggct cctccggctc agcgtcgggc tcgagcaccc cgacgacgtc
     3121 atcgaggacc tggcgcaggc gctcggctag gctccggggg tccgaggccc ctgggagtca
     3181 gcgggggtgg aggacgtccg ggttcagcgc gcggggcggg gtcttgccgt ccaggtaggc
     3241 gacgatggcc tcgaccgcgt ggccgaagaa ggcgtcgtac gcctcgcggg tgacgtagcc
     3301 gatgtgcggc gtgagcacga cgttgtcgag cgcgagcagc gggtgtgagg ccggcagcgg
     3361 ctcgtcgtcg aacacgtcga gcgcggcgcc ggcgatgcgg cgcccgcgaa gggccgccac
     3421 cagcgcggcc tcgtccacga tgggaccccg cgcggtgttc acgaggtagg cggtggactt
     3481 catcatggcc agctcacggg cggtgatgag cccgcgcgtc ttcccgctga ggcgcgtgtt
     3541 gatcgacacc acgtcgctct cgcgcaggag ggtctccagc gggacgaacc tggcgccgcc
     3601 ggcggccgcg cgctcggccg tcagcgtggg tccccacgcg agcaccttca tgccgaggaa
     3661 cgtcccgaac gccgcgatgt gcccgccgat gcgacccatc cccacgaccc ccagcgtctt
     3721 gcccgccagc tcggtgccca ccgaggtctg ccagcggccc tcgcgcaggg cgcgatcctc
     3781 ctgagggatg cgccgcacga gcgccaggag cagcgccatc gtgtgctcga ccgccgccgc
     3841 ggcgctgccg gcggtctcgg tgaccagcac gcggcgcgac gtggccgcga gcacgtccac
     3901 gtggccggag ttgcggccgg tcagcgcgag cagctcaagg cgcggcagcc gctcgagcag
     3961 ggaggccgga aagcgtgtgc gctcgcggat cggaacgatg atctcgtagg gcgcgagccg
     4021 ctgggccagc gccgcttcgt tcttcagcgt gtcccggaag acgtcgaggc gcgcgcgccc
     4081 ctcgagccgc gtccagtacg ggagcgctgc cgccgcaccc tggtagtcgt cgaggacggc
     4141 gacgcgcttc accgctacca gctcacggcg tgatcgagcg agacctcgaa ccgcgcccgc
     4201 gcgtggacca cgcggaggcg cagtcctcga ggcgactcgc tcgccaggcg ggcgcgatag
     4261 agaccgggct cgctcgccgg tatcaacggc gcgctgagcg gtacgccgtt ggcggcttgg
     4321 ccgagcagcg acacctcggc gccggtgagc ggctggttgc cgctgccgag gagccggatc
     4381 gagtagatcg tgtcgcggtc gccgccggcc tgccggtgga cgtccacgcg cacgccctgg
     4441 atcgtcgcgc ggacctgcgg cgctccgacc acggccggca tggtcgcgat gtcatcgatc
     4501 gcagaagccc cgccggcggg gacggcgggc gcaagcggcg ccggccgacg cggagaggca
     4561 gctctcgcct gcggctccga ccgcgccggg gcggccttcg cctgcgactc cgacctcgcc
     4621 tcgcgtcgga ggggaagccc cggggcatcg gaggcgtccg ggcgctcggc ggcggcgtcc
     4681 ggccgttcgg cctcgcgccc gcgggacgtt gcggccagcc gctgctccag cgccgcgatc
     4741 tccgagcgga gtcgcgcctc gacgcgacgg acctcgtccg gagccgtcac cctctcgcgg
     4801 tctgacgcga ccgtctcgcg tggctccggg ggtgtcgtgg tctcgcgctg cgccgggggt
     4861 ggcgcgaggt cgcgcgagga ggacggcgtg gattcgcgag cgctcgccgt cgcgtgagcg
     4921 ggttcgggcg tgtcggcgcg cgctgctggc ggctcggcgc gctgatcctc gacgcccgca
     4981 cgcagtgacg ccggggtggg acgggcgacg agggcggcca cggccagcac tgccacgccg
     5041 gcaaagcaga aggc
//
LOCUS       DATIAZ010000140        13789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1481234, whole genome shotgun sequence.
ACCESSION   DATIAZ010000140 DATIAZ010000000
VERSION     DATIAZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481234"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VLF19_04900"
     CDS             <1..429
                     /locus_tag="VLF19_04900"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSE92623.1"
                     /translation="LVFDVGPETLARTALGRLAPGDAVNLERPLRLGAALGGHLVLGH
                     VDGAGTVTGVTRVESTARVRIALPGPDLEPYLVPKGSVAVDGVSLTVAALGPSSFEVM
                     LIPHTLEATTLGRLQVGQAVNIETDVIGKYVVRSLAFRGT"
     gene            429..1637
                     /locus_tag="VLF19_04905"
     CDS             429..1637
                     /locus_tag="VLF19_04905"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSE92624.1"
                     /translation="MATFATIEEAVEEIRQGRMLLVVDDEDRENEGDFLMAADKATPE
                     AVNFMAKHGRGLICVPMTGERLDELKISMMVADNTAPMGTAFTETVDARRGVTTGTSA
                     YDRAVTIRTLVDPSTRPEDLTRPGHIFPLRAMPGGVLRRAGHTEAAVDLARLAGCAPA
                     GVICEVLDEEGGMARRSQLDEIAARHRLKMITIKDLIEYRTQKEKLVRRIATTRLPTE
                     FGEFAAVAYESTVYSRVHMALVMGEVAGESPVLVRMHSECLTGDVFRSRRCDCGEQLE
                     KAMMLIQQEGRGVVVYLRQEGRGIGLHNKIRAYELQDLGKDTVEANQALGFKADLRDY
                     GIGAQILVDLGIKNLRVLTNNPKKIVGLEGYGLHFVERVPIEIPANPENRKYLSTKRD
                     KLGHLLSSLP"
     gene            1717..2340
                     /gene="ribH"
                     /locus_tag="VLF19_04910"
     CDS             1717..2340
                     /gene="ribH"
                     /locus_tag="VLF19_04910"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSE92625.1"
                     /translation="MAGRAPASRRTTPHDGARFAVVAARFNEQIVKRLVDGALHAFAA
                     AGLPPQAVETHWVPGSFELPQAAALLAATKRYAGIACVGVVVRGQTPHFEHVAREAAA
                     GIRHVALATGVPVTFGVITALSEQQAWERAGGEVGNRGEEAADAALEMAAFVRHVRSP
                     GDPAPPSGRRAPPQGGGGATGGMRWPRTAKWLDARSAQRRLKKAPGK"
     gene            2340..2756
                     /gene="nusB"
                     /locus_tag="VLF19_04915"
     CDS             2340..2756
                     /gene="nusB"
                     /locus_tag="VLF19_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSE92626.1"
                     /translation="MGRRRKAREVALQFLYQLDLHSEDDPSPHAEEFWSRHPVDADTR
                     RFADALVRGAKENQAKADDLIRHYVEHWDLERMAVVDRNILRLAIYELLWAEGVPAKV
                     AINEAIEIAKKFGTRESSRFINGVLDRIHKELRQAS"
     gene            2766..3491
                     /locus_tag="VLF19_04920"
     CDS             2766..3491
                     /locus_tag="VLF19_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSE92627.1"
                     /translation="MRYAILSDVHGNLEALQAVLADARERADGYLCLGDVVGYGADPG
                     PCVELVAEHCEGVVAGNHERAVTGMASLDWFNPWARAAVEWTRARLSPEAHAWLAALP
                     LVREAADATLVHASPVQPEEWDYLVSSEDGFAAFGAFATRVCFVGHSHLPRSWAQGSW
                     GHEHDAEPGTVTLEAGCRYIVNVGSVGQPRDHDPRAAYAVWDLDARRVEIRRVLYDMP
                     AARAKILGAGLPRFLADRLADGR"
     gene            3557..5026
                     /gene="lnt"
                     /locus_tag="VLF19_04925"
     CDS             3557..5026
                     /gene="lnt"
                     /locus_tag="VLF19_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HSE92628.1"
                     /translation="MAAGAALPLAYPRTDWAGTAWVALAPVLLLAVTRPARASFGWGW
                     VAGFIFFLLLLRWLNFTFGVYSAIPWPLTWAPTAALAAYCGFYVGLVMLCVSWVAERR
                     GRGRALAAAPFLWVTAEWLRGHLLGGFPWGHLGYSQHSQLAVIQVAELAGVDGVALVV
                     VAVNAALAGCVTLSRGRAAAGLAAAAVLLAGTIGFGLTRLAEPEPLAEARVALMQPAI
                     EQPLKWDEKHTASTLRIYGELTRSAAQLRPALIVWPETASPTLLRRDRVLLDALSDLA
                     RASGVTLLVGSVDVMDPAGRDVRNTAFLLTEQGIVGRYDKIHLVPFGEYVPLSRVIGF
                     VRGWAQFISELTPGSHAMVFPGPPAPFGVVICYEGIFPALFRRFVQDGARLMVNMTND
                     AWFGRTSGPLQHLAMYPFRAVEHRTALVRVANTGVSAFIDPRGRIVSSLGLFERGILS
                     HEIALRRSETLYTRFGDWLAGLSAGVSALALIAAASRRM"
     gene            5026..6130
                     /gene="prfB"
                     /locus_tag="VLF19_04930"
     CDS             join(5026..5085,5087..6130)
                     /gene="prfB"
                     /locus_tag="VLF19_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_141201661.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSE92629.1"
                     /translation="MAGEGKREVSELTRRVDELRGIFDLPAKDKRLAEIGVEMGAPSF
                     WEDNRRAQELIRERTELQRVVTRVAELARAAEDLGVLIELAAEAGDGSLDAEIAEGIA
                     RLSRDLDEFELKIMLSGPHDAKACIVSIHPGAGGTESQDWAQMLMRMYLRWCERTGLK
                     AEVVDLLPGEEAGIKSATIEITGDYAYGYLKGETGVHRLIRISPFDASKRRQTSFASL
                     HVVPEVDDVEVNVREDEIRVDVFRSSGPGGQGVNTADSAVRITHLPTGMVVQCQNERS
                     QLRNRDTAMRILKARLFEIAHRKQREELAELTGEKKEIAFGSQIRSYTFHPYQLVKDH
                     RTGVEVGNVEGVMDGDIDAFVKAYLMQGRGGSA"
     gene            6127..7614
                     /gene="lysS"
                     /locus_tag="VLF19_04935"
     CDS             6127..7614
                     /gene="lysS"
                     /locus_tag="VLF19_04935"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSE92630.1"
                     /translation="MKGHEASGGDLIRQRHEKLDGLRRRGIDPFGGRFPVTHWAGPLH
                     AQLGEAREEELKAVGGVSLAGRVVALRDHGKSCFAHLMDRTGRVQLYARADQLADDYA
                     LFRDLDVGDFVGVTGEVFRTRTGELTVGVKAFTFLAKSLRLLPEKWHGLKDVETRYRQ
                     RYVDLVVNPDVREIFLLRSRVVAALRRFLDARGFLEVETPMMQPIPGGAIARPFKTHH
                     NALGMDLYLRIAPELYLKRLVVGGFDRVYEINRNFRNEGISTQHNPEFTMLEFYQAYA
                     DYNDLMDLTEALFVELAQALKGGLRLTWGEHAIDLTPPWRRLPFFAGLSQALDQEVST
                     ETPAGVLTGAAATRGIALKPGGAAWEVWKEVFETLVEPTLIQPTFVTDFPIELSPLAR
                     KKRDQPALVERFELFVGRREMANAYSELNDPVDQLARFREQAALQARGDEEAHWLDED
                     YVRALEYGMPPASGEGVGIDRLVMLFADQPSIREVILFPLLRPES"
     gene            7611..8846
                     /locus_tag="VLF19_04940"
     CDS             7611..8846
                     /locus_tag="VLF19_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92631.1"
                     /translation="MRRGLPVELFLGLRYLRAKGQRTNLSLFVWIGVGGVFLGVAALI
                     LVLAVMTGFQDSIRDKIIEANPHLLVFGLGGGGLADSDALAARLRRVPGVRVATPFVL
                     QQALFTTEGGGAHGGLVRGVDLGAAAVQADLRAQLRTGSLAPAAEGQPVILLGVELAR
                     TLGVIAGDQVTVISPKGALTAVGMVPKMRRYTVAGTVQVGMHEYDASIAYLALPVARE
                     FAGLPAGVTGIEVKLTDPFDARRVGARIGEEVGVGFWIRNWMDMNRNLFAALQLEKLA
                     LFVIVTIIVLVAAFAIIGHLVLLVAEKRKEIGILKAIGASSGNVTTVFFAVGMTIGVV
                     GTIAGSVVGLTLIWVQNTYKIIRLAGDVYQIDHLLMKLTWPDFLMIIGATLLLSFLAT
                     ILPARRAGALAPVDVLRYE"
     gene            8921..9613
                     /locus_tag="VLF19_04945"
     CDS             8921..9613
                     /locus_tag="VLF19_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92632.1"
                     /translation="MPDPLLAGEDLEKEFRTGPEVVRVLRGASVSIAPAEVVALVGAS
                     GVGKSTLLHLLGTLDRPTAGRVLFEGQDVFGWGEAGLVRFRRHQVGFVFQFYNLLGEM
                     TALENAMIPALLARRPVREAQARACDALAEVGLGDRRHHRPGELSGGEQQRVAIARAL
                     VNEPRVILADEPTGNLDPKTSAVIFDLFLRLQAERRIAFLIATHNPELARRADRAYSL
                     VEGRAREVRPGE"
     gene            9787..12234
                     /locus_tag="VLF19_04950"
     CDS             9787..12234
                     /locus_tag="VLF19_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017553278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HSE92633.1"
                     /translation="MFERFTERARRVIILAREEAGRFRHDFVGTEHVLLGLIRDGEGI
                     ATAVLQRLGLRLETVKAEVERALAGFPKTLTFGEVPFTPQAKRVLELSIEEARQLGHN
                     YIGTEHLLLGLMKEGQSIAAKILESLGARLDEVRQETLALLGDQYYPRPKKRSQTPVL
                     DEFARDLTQLARESKLDPVIGREQEIERVIQILARRTKNNPVLIGEPGVGKTAIVEGL
                     AQKIISHDVPDVLLNKRLLQLDLGALVAGTKYRGQFEERLKAVMKEIRQSENVILFLD
                     ELHTLIGAGAAEGAIDASNMLKPALSRGEIQTIGATTLDEYRKYIEKDGALERRFQPV
                     IVKAPSVPEAIEIIRGLRHKYEAHHRVKITEQAITAAVNLADRYITDRQLPDKAIDVI
                     DEASSRTRLMALTPPPEVKEIDKELERVIREKDMYLEAQEFEKAAALREKEKLLRHRE
                     EELKREWERNKGKGVQSVEEEDIEFIVSRWTGIPLSKLEEKESEKLARMEEALHGRII
                     GQHDAVAAVSRAIRRSRAGLKDAKRPVGSFVFLGPTGVGKTELARALAEYLFGDENAL
                     IRVDMSEYMEKFSVSRLLGAPPGYVGYEEGGFLTEKVRRRPYSVVLFDEIEKAHPDVF
                     NMLLQVLDDGRLTDSLGHVVDFKNTILIMTSNLGTSLIGKKVSPGFLAESDEASYEKM
                     KDRVMDELKRSFRPEFLNRIDDTIVFHSLSVEHIKLIIRLMVRRINTQLSERGIELVL
                     TPAAEDLLVEKGYDATYGARQLRRTIQKHVEDPLAEAIVRGQMAESARIEVDVEGGTF
                     VFREVRAADAPLELAEH"
     gene            12264..>13789
                     /gene="bamA"
                     /locus_tag="VLF19_04955"
     CDS             12264..>13789
                     /gene="bamA"
                     /locus_tag="VLF19_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HSE92634.1"
                     /translation="MTSAGRTQLSVLLACLGLVLHAGGVRAQQSQRPIPVKEILVEGN
                     RRVQEAVILGRVQTKVGAPFSPGQLSEDIRAIFALGFFDDVQLKVEDFEGGVRVTFAV
                     VERPFIRDVEFSGNKKMATSELQDKIDIKLGSVYNPVEVQKAREKLVEAYESEGYFEV
                     QVTPEVEKFPDGDVKVVFSVNEGRRITIDSIVIHGNEGLSDRQIRRALKTQEREFLIL
                     RGKLVRQYLDEDVERIIALYNDHGYIQARVEGYDVAVNREKARVTLTFTVVEGPQYRV
                     DALRITGATLFPESEVNRLIQLKPGDVYSRSKLRESVRQIQDLYSTVGRAAADIQPRT
                     ELMPDARVSLIFEIAEGPEVYVERINISGNLRSQDKILRREIPMAEGDLFTLQKLQRG
                     RQRLVNLGYFEAVNVSTAPGSDRTKIIINVEVIERPTGLFSIGGGYSSVDNLLGTIDL
                     AQRNFLGRGWEVSMRIRIGGQTQQGIISFTEPWLFDRPLAAGFDLFNLRRVYDDYDYD
                     S"
BASE COUNT         2150 a         4729 c         4819 g         2091 t
ORIGIN      
        1 ctcgtcttcg acgtcggccc cgagacgctc gcgcggacgg cgctcgggcg cctggcgcca
       61 ggggacgccg tgaacctcga gcgcccgctt cgcctgggcg ccgccctggg cggccacctg
      121 gtcctcggac acgtcgatgg cgcgggcacc gtgacgggtg tgacccgtgt agaatcgacg
      181 gcacgggtca ggatcgccct gcccggaccg gacctcgagc cgtacctcgt acccaagggc
      241 tcggtggcgg tcgacggggt gagcctcacg gtggcggcgc tgggcccgtc gagcttcgag
      301 gtgatgctga tcccgcacac gctcgaggcg acgacgctcg ggcggctcca ggtggggcag
      361 gcggtgaaca tcgagaccga cgtgatcggc aagtacgtgg tgcggtcact cgcgttccgg
      421 gggacgtgat ggccacgttc gcgaccattg aagaggccgt cgaggagatc cggcagggcc
      481 ggatgctcct cgtcgtcgac gacgaggatc gggagaacga gggcgacttc ctcatggccg
      541 ccgacaaggc gacgccggag gccgtcaact tcatggccaa gcacggccgg gggctcatct
      601 gcgtgcccat gacgggcgaa cggctggacg agctgaagat ttccatgatg gtcgccgaca
      661 ataccgcgcc gatgggcacg gccttcaccg agaccgtgga cgcgcgccgc ggtgtgacca
      721 ccggaacctc ggcctacgac cgcgccgtga ccatccgcac gctcgtcgat ccctcgacca
      781 ggcccgagga cctcacccgg cccggccaca tctttccgct ccgcgccatg cccggcggcg
      841 tactgcggcg cgccggccac accgaggcgg ccgtcgacct ggcgcgtctg gccggctgcg
      901 cgcccgcggg cgtgatctgc gaagtgctcg acgaggaggg cgggatggcc cgccggtcgc
      961 agctcgacga gatcgcggcg cggcaccggc tcaagatgat cacgatcaag gacctcatcg
     1021 agtaccggac ccagaaggaa aagctcgtcc gccgcatcgc gacgacgcgg ctgcccaccg
     1081 agttcggcga gttcgccgcg gtcgcctacg agtccaccgt ctacagccgc gtgcacatgg
     1141 cgctcgtcat gggcgaggtg gccggcgagt cgcccgtgct cgtgcgcatg cactccgagt
     1201 gcctgaccgg cgacgtcttc cgctcccgcc ggtgtgactg cggcgagcag ctcgagaaag
     1261 cgatgatgct catccagcag gaagggcgag gcgtcgttgt ctacctccgc caggaggggc
     1321 ggggcatcgg gctgcacaac aagatccgcg cctacgagct ccaggacctg ggcaaggaca
     1381 cggtcgaggc caaccaagcc ctgggcttca aggcggacct gcgcgactac ggcatcggcg
     1441 cccagatcct cgtggacctc gggatcaaga acctgcgcgt gctcacgaac aatcccaaga
     1501 agatcgtcgg cctcgagggc tatgggctcc acttcgtcga gcgcgtgccg atcgagattc
     1561 cggccaaccc cgagaaccgc aagtacctga gcacgaagcg cgacaagctc ggccacttgc
     1621 tgtcctcgct cccgtagtgc ggcctcgggt ggtgcgtcca gcgcgcgagc cgcgaaggtg
     1681 tcgggcgggg cgtccgggcc ccctcgacgt ggactgatgg cgggccgcgc gccggcctca
     1741 cgccgcacga caccgcacga cggtgcccgc ttcgccgtcg tcgccgcacg cttcaacgag
     1801 cagatcgtca agcgtctcgt cgacggcgcg ctgcacgcct tcgcggcggc cggcctgccc
     1861 ccgcaggccg tcgagaccca ctgggtgccg ggttccttcg agttgccgca ggcggcggcc
     1921 ctcctggcgg cgaccaaacg ctacgccgga atcgcctgcg tcggcgtcgt ggtccgcggg
     1981 cagacaccgc acttcgagca cgtcgcccgg gaggctgcgg cgggcatccg ccacgtggcg
     2041 ctcgcgacgg gcgtgcccgt caccttcggg gtcatcaccg cgctctccga gcagcaggcg
     2101 tgggagcgcg cgggcgggga ggtcggcaac cgcggcgagg aggccgctga cgcggccctc
     2161 gagatggccg ccttcgtgcg gcacgtgcgg tctccgggag atcccgcccc accctcgggc
     2221 cgtcgtgcgc cgccgcaggg gggaggcgga gccacgggcg gaatgcgctg gccgcgaacg
     2281 gcgaaatggc tcgacgctcg ctctgcgcaa cggcggctca agaaagcgcc gggcaagtaa
     2341 tgggccgacg ccggaaggcc cgagaggtgg cgctgcagtt cctctaccag ctcgacctcc
     2401 actccgagga cgatccgtcg ccgcacgccg aagaattctg gagccggcac cccgtcgacg
     2461 ccgacacccg ccggttcgcc gacgcgctcg tgcggggcgc caaggagaac caggcgaagg
     2521 ccgacgacct gatccgccac tacgtcgagc actgggacct cgagcggatg gccgtcgtcg
     2581 accgcaacat cctccgtctc gcgatctacg agctcctgtg ggcggagggg gtgccggcca
     2641 aggtggccat caacgaagcg atcgagatcg ccaagaagtt cggcacgcga gagtccagcc
     2701 ggttcatcaa cggagtcctg gaccggatcc acaaagagct ccgccaggcg tcctgaggtt
     2761 tcggcgtgcg gtacgccatc ctgtccgatg tccacggcaa tctggaggcg ctgcaggccg
     2821 tcctggccga cgcgcgcgag cgcgccgacg gctatctctg tctgggcgac gtggtcggct
     2881 acggtgccga tcccggcccg tgcgtggagc tcgtggccga gcactgcgag ggcgtcgtcg
     2941 ccggcaacca cgagcgcgcc gtgaccggca tggcctcgct cgactggttc aatccgtggg
     3001 cgcgcgccgc cgtcgagtgg acccgggccc gtctctctcc cgaagcgcat gcgtggctcg
     3061 cggcgctgcc gctcgtgcgc gaggcggcgg acgcgaccct ggtccacgcc tcgccggtgc
     3121 agcccgagga atgggactat ctggtctcct cggaggacgg gttcgccgcg ttcggcgcct
     3181 tcgccacccg cgtgtgcttc gtcggtcact cgcatctgcc gcgctcgtgg gcgcagggat
     3241 cctggggcca cgagcacgac gcggagccgg gaacggtgac gctcgaggcc gggtgccggt
     3301 acatcgtcaa cgtgggcagc gtcggccagc cgcgcgacca tgatccccgc gccgcatatg
     3361 cggtctggga tctcgacgcg cgccgggtcg agatccgtcg cgtcctctac gacatgccgg
     3421 cagcccgggc gaagatcctc ggcgccggcc tgccgcgctt tctggccgac cgcctggccg
     3481 acgggcgctg aggacctccc tgcgcgaggc cgtcgccgac gcgctgcgcc gggctctgcc
     3541 cgcggcgctc ctggccgtgg ccgccggcgc cgccctccca ctggcgtatc cgcgcaccga
     3601 ctgggcgggc acggcctggg tcgcgctggc gcccgtgctg ctgctggcgg tcacgcggcc
     3661 ggccagggcc agcttcggct ggggctgggt ggcaggcttc atcttcttcc tgctcctgct
     3721 gcgctggctc aacttcacgt tcggcgtcta cagcgcgatc ccgtggccgc tcacctgggc
     3781 gcccacggcg gccctcgccg cgtactgcgg cttctacgtg ggactcgtca tgttgtgcgt
     3841 gtcgtgggtc gcggagcgac gcggccgcgg ccgggcgctg gcggccgcac cctttctctg
     3901 ggtgaccgcc gagtggctgc gcggacacct cctggggggc tttccgtggg gtcacctcgg
     3961 gtactcgcaa cacagccagc tcgcggtcat ccaggtggcc gagctcgccg gcgtcgacgg
     4021 cgtggccctg gtcgtcgttg ccgtgaacgc ggccctggcc gggtgcgtca ccctgtcgcg
     4081 cggccgggcc gccgccgggc tggccgcggc ggccgtcctc ctcgctggca cgataggctt
     4141 cggcctcacg cgcctggccg agcccgagcc cctggccgag gccagggtcg ccctgatgca
     4201 gcccgcgatc gagcagccgc tgaagtggga cgagaagcac accgcctcca cgctccggat
     4261 ctacggtgag ctgacccggt cggcggcaca gttgcggccg gcgctcatcg tctggccgga
     4321 gacggcatcg cccaccctgc tccggcgtga ccgggtcctc ctcgacgcgc tctcggatct
     4381 cgcgcgagcc tccggcgtga cactgctcgt gggtagcgtg gacgtcatgg acccggcggg
     4441 ccgcgacgtg cgcaacaccg catttttact caccgaacaa ggtatcgtcg gcaggtatga
     4501 taagatccac ttggttccgt tcggagagta cgtgccgctg tcgagggtca tcgggttcgt
     4561 gcggggctgg gcgcagttca tctccgagct gacgccgggc tcacacgcca tggttttccc
     4621 gggtccgcca gccccgttcg gcgtcgtgat ctgttacgag ggcatcttcc ccgcgctgtt
     4681 ccgtcggttc gtgcaggacg gcgcgcgctt gatggtgaac atgacgaacg acgcgtggtt
     4741 cggccgcacc agcgggccgc tgcagcacct ggcgatgtac ccgttccggg ccgtcgagca
     4801 ccgcaccgcc ctggtccgcg tcgccaacac cggcgtgtcc gcgttcatcg atccgcgcgg
     4861 acggatcgtg agctcgctcg gactcttcga gcgcggcatc ctgagtcacg agatcgcgct
     4921 gcgccggagc gagaccctgt acacgcgctt cggcgactgg ctggcggggt tgtcggcggg
     4981 ggtgtcggcc ctggcgctga tcgcggcggc gtcgcggagg atgtgatggc gggcgaggga
     5041 aagcgcgagg tgagcgagct gacccggcgt gtcgacgagc tccggaggca tctttgacct
     5101 gcccgccaag gacaagcggc tggccgagat cggcgtcgag atgggcgcgc catccttctg
     5161 ggaggacaac cgccgcgcgc aggagctgat ccgcgagcgt acggagctgc agcgcgtggt
     5221 gacgcgcgtc gccgagctcg cccgcgcggc ggaggacctg ggcgtgctca tcgagctcgc
     5281 cgccgaagcc ggagacggca gcctcgacgc ggagatcgcc gagggcatcg cgcgcctttc
     5341 gcgggatctc gacgagttcg agctgaagat catgctgtcc ggcccgcacg acgccaaggc
     5401 gtgcatcgtg tcgatccatc ccggcgccgg gggcaccgag tcgcaggact gggcgcagat
     5461 gctcatgcgc atgtacctcc gctggtgcga gcgcaccggc ctcaaggccg aggtcgttga
     5521 cctgctgcct ggcgaggagg cgggcatcaa gtccgcgacc atcgagatca ccggcgacta
     5581 cgcctacggc tatctcaagg gcgagacggg cgtccaccgg ctcatccgca tctcgccctt
     5641 cgacgcctcc aagcgccgtc agaccagctt cgcctcgctg cacgtggtgc ccgaggtcga
     5701 cgacgtggag gtgaatgtcc gcgaggacga gatccgtgtg gacgtcttcc gatcgtccgg
     5761 tcccggcgga cagggcgtca acaccgccga ctcggccgtc cgcatcacgc acctgcccac
     5821 gggcatggtc gtgcagtgtc agaacgagcg ctcacagctc cggaaccgcg acaccgcgat
     5881 gcggatcctc aaggcccgcc tcttcgagat cgcccacagg aagcagcggg aggagctggc
     5941 cgagctgacc ggcgagaaga aggagatcgc cttcggcagc cagatccgct cctacacctt
     6001 ccacccctac cagctcgtca aggaccatcg gacgggcgtg gaggtgggca acgtcgaggg
     6061 cgtgatggac ggtgacatcg acgcgttcgt caaggcgtac ctgatgcagg ggcggggagg
     6121 ctccgcgtga agggacacga ggcatccggc ggcgacctga tccgtcagcg tcacgagaag
     6181 ctcgacggtc tgcgccggcg tggcatcgac cccttcggcg ggcggtttcc cgtgacccac
     6241 tgggcggggc cgctccacgc gcagctcggc gaggccagag aggaggagct caaggccgtc
     6301 ggcggcgtga gtctggccgg acgcgtcgtg gcgctgcgcg atcacggcaa gtcctgcttc
     6361 gctcacctca tggaccggac cggtcgcgtc cagctctatg cccgcgccga ccagctcgcc
     6421 gacgactatg ccctgttccg ggacctggac gtcggcgact tcgtcggcgt gaccggcgag
     6481 gtcttccgta cgcggacggg cgagctgacc gtcggcgtca aggccttcac gttcctcgcc
     6541 aagtccctgc ggttactgcc cgagaagtgg catggcctca aggacgtcga gacgcggtac
     6601 cgccagcgat acgtggacct cgtcgtgaac cccgacgtcc gcgagatctt cctgctgcgc
     6661 tcgcgcgtgg tggccgcgct gcggcgcttc ctggacgctc gcggcttcct cgaggtcgag
     6721 acgcccatga tgcagccgat tcccgggggc gccattgccc ggcccttcaa gacgcaccac
     6781 aacgcgctgg gcatggacct ctatctccgc atcgcccccg agctctatct gaagcgcctc
     6841 gtggtcggcg gcttcgatcg cgtgtacgag atcaaccgca acttcaggaa cgagggaatc
     6901 tccacgcagc acaatcccga gttcacgatg ctcgagttct accaggcgta cgcggactac
     6961 aacgacctca tggacctgac cgaggcactg ttcgtggagc tggcgcaggc cctcaagggc
     7021 gggctccgac tgacctgggg cgagcacgcc atcgacctca cgccgccctg gcggcgcctg
     7081 ccgttcttcg ccgggctctc ccaggcgctc gaccaggagg tcagcacgga gaccccggcc
     7141 ggcgtcctga cgggcgcggc cgccacgcgc gggatcgcgc tgaagccggg cggggcggcg
     7201 tgggaggtct ggaaagaggt cttcgagacg ctggtggagc cgacgctgat ccagcccacg
     7261 ttcgtgaccg acttcccgat cgagctgtcg ccgctggcga ggaagaagcg cgatcagccg
     7321 gctctggtcg agcggttcga gctcttcgtc gggcggcgcg agatggccaa cgcctacagc
     7381 gagctcaacg accccgtgga ccagctggcg cgcttccgcg agcaggcggc cctgcaggcg
     7441 cgcggcgacg aggaggcgca ctggctggac gaggactacg tgcgggcgct ggagtacggc
     7501 atgccgccgg cctccggcga gggggtcggc atcgaccgcc tggtcatgct cttcgccgac
     7561 cagccgtcga tccgcgaggt catcctgttc ccgctcttga ggccggagtc gtgaggcgcg
     7621 gcctcccggt cgagctgttc ctgggactgc gctatctgcg ggcgaagggg cagcgcacga
     7681 acctctcgtt gttcgtgtgg atcggcgtgg gtggcgtctt cctcggcgtg gccgcgctga
     7741 tcctcgtgct cgcggtcatg accggcttcc aggactcgat ccgcgacaag atcatcgagg
     7801 ccaatcccca cctcctcgtg ttcggcctgg ggggcggcgg cctcgcggat tccgacgcgc
     7861 tggccgcgcg gctgcgccgg gtgcccggcg tgcgcgtggc cacgccgttc gtgctccagc
     7921 aagcgctctt cacgaccgag ggcggcggcg cgcacggcgg cctcgtgcgc ggcgtggatc
     7981 tcggcgcggc ggcggtgcaa gcggacctgc gggcccagct caggacgggc agcctggcgc
     8041 cggcggccga gggccagccg gtgatcctgc tgggcgtcga gctggcgcgc acgctgggcg
     8101 tgatcgctgg cgatcaggtc accgtgatct cgccgaaggg cgcgctcacc gcggtgggca
     8161 tggtgcccaa gatgcggcgg tacaccgtcg ccggcaccgt gcaggtgggc atgcacgagt
     8221 acgacgcctc gatcgcatat ctggcgctgc cggtggcgcg cgagttcgcc gggctgcccg
     8281 ccggcgtgac gggcatcgag gtgaagctga ccgacccctt cgatgccagg cgcgtcggag
     8341 cccgcatcgg cgaggaggtc ggcgtgggtt tctggatccg caattggatg gacatgaacc
     8401 gcaacctctt cgccgcgctc cagctcgaga agctcgcgct gttcgtgatc gtgaccatca
     8461 tcgtgctcgt ggcggcgttc gcgatcatcg gccacctggt gctgctcgtc gccgagaagc
     8521 gcaaggagat cggcatcctc aaggcgatcg gggcctccag cggcaacgtc acgaccgtct
     8581 tcttcgcggt gggcatgacg atcggcgtcg tgggcaccat cgccggctcc gtggtgggcc
     8641 tgacgctgat ctgggtgcag aacacctaca agatcatccg cctcgccggt gacgtctacc
     8701 agatcgacca cctcctgatg aagctcacgt ggccggactt cctgatgatc atcggggcca
     8761 ccctgttgct ctcgtttctg gccacgatcc tgcccgcccg ccgcgccggc gccctggccc
     8821 cggtggacgt gctgagatat gagtaagcgc gcgcgctcgg ccgaccgtgg ggcggcggag
     8881 ccctgcgccc tctcgccgcg cctggagccc cggaggtggc atgcctgacc ccttgctcgc
     8941 gggcgaggac ctcgagaagg agttccggac cggccccgag gtggtccgcg ttcttcgcgg
     9001 cgcgagcgtc agcatcgccc cggccgaggt cgtggccctc gtcggcgcct ccggggtggg
     9061 caagtccacg ctgctgcacc tgctgggcac gctcgaccgt cccacggcgg ggcgggttct
     9121 cttcgagggg caggacgtgt tcggctgggg cgaggccggc ctcgtgcgct tccgccgcca
     9181 ccaggtcggc ttcgtgttcc agttctacaa cttgctcggc gagatgaccg cgctcgagaa
     9241 cgcgatgatt cccgcgctgc tggcccgccg tcccgtccgc gaagcccagg cgcgtgcctg
     9301 cgacgccctc gccgaggtcg ggctcgggga ccgccgccac caccggcccg gagagctctc
     9361 cggcggcgag cagcagcggg tggccatcgc gcgcgcgctc gtcaacgagc cgcgggtgat
     9421 cctcgccgac gaaccgaccg gtaacctcga ccccaagacg agcgcggtca tcttcgacct
     9481 gttcctgaga ctgcaggccg agcgccgcat cgccttcctg atcgccaccc ataacccgga
     9541 actggcgcgg cgagcggacc gggcctactc gctggtcgag ggtcgtgccc gcgaggtccg
     9601 gcccggtgag tgacctgggg cggagtccct ggcgcgtttc ccgcaagtct ttcgcagagt
     9661 tagtcgtctg gatcgaaaat cactggcggc tcgcccgggg ggagggctat actgaggccg
     9721 gtaactagcc gtcggggaaa gcaaaaccgg caccccggag gcgacgaaag gggtgggggg
     9781 actccagtgt ttgaacgatt cactgaacgg gcgcggcggg tcatcatctt ggcgcgtgaa
     9841 gaggcgggac ggttccgtca cgatttcgtg ggtacggagc acgttctcct cgggctcatc
     9901 cgcgacggcg agggcatcgc gacggcggtg ctccagcgcc tcgggctgcg gttggagacg
     9961 gtcaaggcgg aggtggagcg cgccttggcg ggcttcccga agacgctcac gttcggtgaa
    10021 gtgcccttca ccccgcaagc caagcgggtg ctcgagctct ccatcgaaga ggcgcgtcag
    10081 ctcggccaca actacatcgg caccgagcac ctgctgctcg ggctcatgaa ggaaggccag
    10141 tcgatcgccg cgaagatcct cgagtcgctc ggcgcacggc tggacgaggt gcgccaggag
    10201 acgctggcac tgctgggcga ccagtactat ccgcgtccga agaagcggtc gcagacgccg
    10261 gtgctcgacg agttcgcccg cgacctcacc cagctcgccc gggagagcaa gctcgatccg
    10321 gtcatcgggc gggagcagga gatcgagcgc gtcatccaga tcctcgcgcg ccggaccaag
    10381 aacaacccgg tgctgatcgg cgagcccggc gtgggcaaga cggcgatcgt cgagggcctc
    10441 gcgcagaaga tcatcagcca cgatgtcccc gacgtgctcc tgaacaagcg cctgctccag
    10501 ctggacctgg gggccctggt ggccggcacg aagtaccgcg gtcagttcga ggagcggctc
    10561 aaggcggtga tgaaggagat ccggcagtcg gagaacgtca ttctgttcct cgacgagctg
    10621 cacacgctga tcggcgccgg cgccgccgag ggcgcgatcg acgcctccaa catgctgaag
    10681 ccggccctct cgcggggtga gatccagacg atcggcgcga ccactctgga cgagtaccgc
    10741 aagtacatcg agaaggacgg agcgctcgag cgccgcttcc agcccgtgat cgtcaaggcg
    10801 cccagcgtgc cggaggcgat cgagatcatc cgcggtctgc gccacaagta cgaggcccac
    10861 caccgcgtga agatcaccga gcaggccatc accgccgccg tgaacctggc ggaccgctac
    10921 atcaccgatc ggcagctccc cgacaaggcc atcgacgtga tcgacgaggc ctcctcgcgc
    10981 acgcggctca tggccctgac ccccccgccg gaggtcaagg agatcgacaa ggagctggaa
    11041 cgggtcatcc gcgagaagga catgtacctc gaggcccagg agttcgagaa ggccgccgcc
    11101 ctccgcgaga aggagaagct gctccgccat cgcgaagagg agctcaagcg cgagtgggag
    11161 cgcaacaagg gcaagggcgt ccagtcggtc gaggaagaag acatcgagtt catcgtctcc
    11221 cgctggaccg gcatcccgct gagcaagctc gaggagaagg aatccgagaa gctggccagg
    11281 atggaggagg ccctccacgg ccggatcatc gggcagcacg acgccgtggc tgcggtctca
    11341 cgcgccatcc ggcgctcgcg ggccgggctc aaggacgcca agcgtccggt cgggtccttc
    11401 gtgttcctcg gaccgaccgg cgtgggcaag acggagctgg cgcgcgcgct cgccgagtac
    11461 ctcttcggcg acgagaacgc gctgatccgc gtggacatgt ccgagtacat ggaaaagttc
    11521 tcggtctcgc ggctgctcgg cgcgccgccc ggctacgtcg gctacgagga gggcgggttc
    11581 ctgaccgaga aggtccggcg gcgcccgtac tcggtggtgc tgttcgacga gatcgagaag
    11641 gcgcaccccg acgtcttcaa catgctcctc caggtgctcg acgacggccg gctgaccgac
    11701 tcgctgggcc acgtggtgga cttcaagaac acgatcctga tcatgacgtc gaacctgggg
    11761 acgagcctga tcggcaagaa ggtgtctccc ggcttcctgg ccgagagcga cgaggcgtcc
    11821 tacgagaaga tgaaggaccg cgtgatggac gagctcaagc gctcgttccg gccggagttc
    11881 ctgaaccgga tcgacgacac catcgtgttc cactcgctgt cggtcgagca catcaagctg
    11941 atcatccggt tgatggtccg ccggatcaac acgcagctct ccgagcgggg catcgagctc
    12001 gtgctcacgc ccgccgccga ggatctgctc gtcgagaagg gctacgacgc gacgtacggc
    12061 gcgcgccagc tgcggcgcac gatccagaag cacgtcgagg acccgctcgc cgaggcgatc
    12121 gtgcgggggc agatggcgga gagcgcgcgg atcgaggtgg acgtcgaagg aggcacgttc
    12181 gtcttccgcg aggtgcgcgc ggccgacgcg cccctcgaac ttgccgagca ctagacgacc
    12241 gtccacgcga gcctgagcgt ccggtgacca gtgcggggag aacgcagctc tccgtcttgc
    12301 tcgcctgtct cggactcgtg ctccacgccg gcggtgtccg ggcccagcag tcccagcggc
    12361 ccatccccgt caaggagatc ctcgtcgagg gcaaccgccg ggtgcaggaa gcggtcatcc
    12421 tcggccgcgt gcagaccaag gtcggcgcgc cgttcagccc gggccagctc agcgaggaca
    12481 tccgggcgat cttcgccctg gggttcttcg acgacgtcca gctcaaggtc gaggacttcg
    12541 agggtggcgt ccgggtgacg ttcgccgtcg tcgagcgccc gttcatccgc gacgtcgagt
    12601 tcagcggcaa caagaagatg gccacatccg agctccagga caagatcgac atcaagctcg
    12661 gcagcgtgta caaccccgtc gaggtccaga aggcccggga gaagctcgtc gaggcctacg
    12721 agtcggaggg ctacttcgag gtgcaggtca cgcccgaagt ggagaagttt cccgacggcg
    12781 acgtgaaggt cgtcttcagc gtcaacgaag gccggcgcat caccatcgac tcgatcgtca
    12841 tccacggcaa cgaggggctg agcgatcggc agatcaggcg ggcgctcaag acccaggagc
    12901 gcgagttcct catccttcgc ggcaagctgg tgcgccagta cctggacgag gacgtggagc
    12961 gcatcatcgc gctctacaac gaccacggat acatccaggc tcgcgtggag ggctacgacg
    13021 tcgcggtgaa tcgcgagaag gcccgggtga cgctgacctt cacggtcgtc gaggggcccc
    13081 agtaccgggt cgatgctctg cggatcaccg gcgcgacgct gttccccgag tcggaggtca
    13141 accgtctgat ccagctcaag cctggagacg tctactcccg cagcaagctg cgggagagcg
    13201 tccgccagat ccaggacctc tacagcaccg tgggccgagc cgccgccgac attcagccca
    13261 ggaccgagct gatgcccgac gccagggtca gcctgatctt cgagatcgcg gaggggcccg
    13321 aggtgtacgt cgagcgcatc aacatctcgg ggaacctgcg ttcgcaggac aagatcctcc
    13381 gccgggagat ccccatggcc gagggcgatc tcttcacgct ccagaagctt cagcgcggcc
    13441 ggcagcgcct cgtcaacctc ggatacttcg aggcggtcaa cgtcagcaca gcgcccggat
    13501 ccgaccggac gaagatcatc atcaacgtcg aggtgatcga gcgccccacc gggctcttct
    13561 ccatcggcgg cggctactcc tccgtggaca acctcctcgg caccatcgac ctggcccagc
    13621 gcaacttcct cgggcggggc tgggaggtct cgatgcggat ccgcatcggc ggccagacgc
    13681 agcagggcat catcagcttc acggagccct ggctcttcga ccgtccgctg gcggccggct
    13741 tcgacctctt caacctcagg cgcgtctacg acgactacga ctacgattc
//
LOCUS       DATIAZ010000141         6286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_954843, whole genome shotgun sequence.
ACCESSION   DATIAZ010000141 DATIAZ010000000
VERSION     DATIAZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954843"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VLF19_04960"
     CDS             complement(<1..281)
                     /locus_tag="VLF19_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92635.1"
                     /translation="MLTDLFAVAHGIAVAAVEAAIPPSCRRGVVQAPPITRDGADTIL
                     EFGPWRPRHPARHLVPSLSPLGRACRFRFEASVRAPGGWSSWAATTTVGG"
     gene            complement(603..1892)
                     /locus_tag="VLF19_04965"
     CDS             complement(603..1892)
                     /locus_tag="VLF19_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSE92636.1"
                     /translation="MSELSDLVRHAARVFPGGVLGSHRNGPGLEFVVREGRGAHLWDT
                     NGRRYIDYLLGSGPMLLGHAHPAVVAAVERQLGRGSTYMLLNEPVIELAEEIVRAVPC
                     AEQVRFTASGSEATFFALRVARAHRQRDKIMKFEGGWHGTHDYGAMSVSTRSPKAFPA
                     PTLDSAGIPSMLQDSVLIAPYNDLATAEALITAHHRELAAVIVEPYQRVIVPAPGFLQ
                     GLRDITRRYGVLLIFDEIVTGFRLAYGGAQEYYGVVPDLASYGKVMAGGFPLAAVAGP
                     ADIMKHFDPALEGTGDYVWQAGTLNGNPIAAAAGLATLTELRKPGTYARLHGVGRRLR
                     DGLAEAARRHGLAAQVSGEAPVFDILFTDREIVDYRATLAADGARIRRFNAECLRRGV
                     VKAVNKIYVSLAHSDADVEETLAVFDSALAVVAEDGR"
     gene            complement(1927..2931)
                     /locus_tag="VLF19_04970"
     CDS             complement(1927..2931)
                     /locus_tag="VLF19_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSE92637.1"
                     /translation="MRAMVLEAPAPITTRPLRERRLAIPEPAAGEVLVRVTACGICRT
                     DLHVIEGELPPHRSPVVPGHQVVGHVERLGAAVVTPRVGGRVGIAWLRRTCGACRFCE
                     KRRENLCERAEFTGWDADGGYAEYAVVPADFAYPIPEIFGDVEAAPLLCAGIIGYRAL
                     RLSGVGRGERLAMYGFGSSAHITLQIAARRGCEVYVFTREATHRALARSLGAAWVGEL
                     GDRAPAPVEGAIIFAPAGELVPLALRALAPGGTLALAGIHMSRIPPVEYADLFHERRV
                     TSVTANTREDGRELLMEASLIPLHPAVTTFPLADANAALERLAVGAFAGSGVLLVDRV
                     "
     gene            complement(2958..3869)
                     /locus_tag="VLF19_04975"
     CDS             complement(2958..3869)
                     /locus_tag="VLF19_04975"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HSE92638.1"
                     /translation="MTAPRPRRSLHFVPGGNERMIARALTLPADGLILDLEDAVAPDR
                     KAATRPIVRGWLEKLDFGGRERWVRMNPVATGLGRADLEETIAGRPDGYVVPKPRSAA
                     DVREIAQALDRLEHEHGIPHGTTRLVLIATETPEGLLNIKEVAAASPRIVAVSWGIED
                     LGAAMGLGRVRDERGQYLDIPRHARVMCAVAAAAAGVQSIDTVYTDIADLDGLRRECQ
                     DGVDMGFTGKISIHPSQIAVINAAFTPAPEAVAEARELCAAFEEHRRRGVYAFTFKGR
                     MVDAPHLAGALRVLARAGETPPTRPGA"
     gene            complement(3866..4333)
                     /locus_tag="VLF19_04980"
     CDS             complement(3866..4333)
                     /locus_tag="VLF19_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSE92639.1"
                     /translation="MAGKHFEELEVGQTYRHQPGRTITEADNVFFSCLTMNPQPLHVD
                     FHAASKAEFGKPLVNSLLTLGIAVGLSVGETTLGTTVGNLGFEKVEFPKPVFHGDTIY
                     AETEVVDKRESRSRPQWGIVTFEHRAVNQHGDVVMRARRNAMMRRRAGEGGGR"
     gene            4538..5749
                     /gene="lhgO"
                     /locus_tag="VLF19_04985"
     CDS             4538..5749
                     /gene="lhgO"
                     /locus_tag="VLF19_04985"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HSE92640.1"
                     /translation="MPERYDVAIVGGGIVALATARALNERAPRARLAILEKEAALAQH
                     QTGHNSGVIHSGIYYKPGSYKARLCVEGAQLMKEFCEAHGIRVDRCGKVIVATSAEEV
                     PRLMTLWERGNANGVPGLALIDPPRLRELEPNTVALKAIHSPNTAIVDFKEVAVALAR
                     GLTAAGVTIETDARVTAIRRDGEGLELTTPRATVHARGLVNCAGLYSDDVVRMAGAEP
                     GVRIVPFRGEYYFLKPERRSLVRGLIYPVPDPEFPFLGVHFTKTVHGDVEAGPNAVLA
                     FAREGYTFGRVRPSELLDTLRYPGFWVMARRYWRTGAYEVYRSLSKAAFVRALQRLVP
                     AVRGDDLSRGGAGVRAQAVTPEGALVDDFRIVETTGAVHVLNAPSPAATASLAIGRHI
                     ASVASERFGLK"
     gene            5916..>6286
                     /locus_tag="VLF19_04990"
     CDS             5916..>6286
                     /locus_tag="VLF19_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92641.1"
                     /translation="MPARRVKQFGVEFYQAGLSAKDIDRLVKFEVLGYSGGPGEEPTK
                     GRSKSRSRVNWDALEKRISESETAYQRPVIRRKIDELVAYYRECKDAGTLPPIPGAVI
                     ITSERRFTFTPVAGQHDVGLL"
BASE COUNT          891 a         2255 c         2254 g          886 t
ORIGIN      
        1 ccgccgacgg tggtggtcgc ggcccaggac gaccagccgc ctggcgcgcg caccgaggcc
       61 tcgaagcgga agcgacaggc acggccgagc gggctcagcg acggcaccag gtggcgggcg
      121 gggtgccgtg gccgccatgg cccgaactcc aggatcgtgt ccgcgccatc gcgtgtgatc
      181 ggcggcgcct gcacgacgcc gcgtcggcac gagggtggga tcgcggcctc cacggccgcg
      241 acggcgatgc catgggcgac ggcgaagagg tcagtgagca cgccggagta ccgccgacgc
      301 gggatcgggg gcgtgggcgg gacgcgcccg cccacggtct ccccccttca tcgtgcccag
      361 ctcagcgagc tgcgcgtccc ggcacgagcc ggggcattgt cggtgctgcg gtctcaagac
      421 gccgctcggg gccgctagca aactccgcgc ggcgcggccg ctatgtccgg tggcgcgttc
      481 tccatccgca cctcctggat aggagtttcc gccacaacgg cgcgcggacg cctgatccac
      541 gacgccgtac ccgtgatgta cgccagacgc ggagccacgt caagcccccg ggtcgcgccg
      601 tgctatcgtc cgtcctcggc aacaacagcg agcgcgctgt cgaagaccgc gagcgtctcc
      661 tcgacgtcgg cgtcgctgtg cgcgagcgag acgtagatct tgttgaccgc cttcaccacc
      721 ccgcgccgca gacactcggc gttgaagcgc cggatcctgg cgccgtccgc ggccagcgtc
      781 gcgcggtagt cgacgatctc gcgatcggtg aagaggatgt cgaagacggg cgcctcgccg
      841 ctcacctgcg cggcgagccc gtggcgccgg gcggcctcgg caagaccgtc ccggaggcgg
      901 cggcccaccc cgtgcagccg cgcgtacgtc cccggcttgc gcagctcggt cagcgtcgcc
      961 agccccgcgg cggccgcgat cgggttgcca ttcagcgtgc ccgcctgcca cacgtagtcg
     1021 ccggtgccct cgagggcggg gtcgaagtgt ttcatgatgt cggccggccc cgccacggcg
     1081 gccagcggaa agccgccggc catcaccttg ccgtaggagg ccaggtccgg cacgacgccg
     1141 tagtactcct gagcgccgcc ataggccagc cggaagcccg tgacgatctc gtcgaagatc
     1201 aacagcacgc cgtagcggcg cgtgatgtcg cggaggccct ggaggaaccc cggcgccggc
     1261 acgatcacgc gctggtaggg ctcgacgatg accgcggcca gctcgcggtg gtgagcggtg
     1321 atgagcgcct cggcggtggc gaggtcgttg tacggcgcga tcagcacgct gtcttgcagc
     1381 atcgacggaa tgccggccga gtccagcgtc ggcgccggga acgccttggg cgagcgcgtg
     1441 ctcacgctca tggcgccgta gtcgtgcgtg ccgtgccagc cgccctcgaa cttcatgatc
     1501 ttgtcgcgct ggcggtgggc gcgggccacg cgcagcgcga agaacgtggc ctcgctgccg
     1561 ctcgcggtga agcgcacctg ttcggcgcac ggcaccgcac ggacgatctc ttccgccagc
     1621 tcgatgaccg gctcgttgag cagcatgtag gtgctgccac ggccgagctg ccgctccacc
     1681 gccgcgacga cggccgggtg ggcgtgcccg agcagcatcg gacccgagcc cagcaggtag
     1741 tcgatgtatc ggcggccgtt cgtgtcccag aggtgcgcgc cgcggccctc gcggacgacg
     1801 aactcgaggc ccgggccgtt gcgatgactg ccgagcacgc cgccgggaaa gacccgcgcc
     1861 gcgtgccgga cgagatcgga gagctcgctc atggagttcc tcgcttcgct gtcgtcggtc
     1921 acgccatcat acgcgatcca ccaggagcac gcccgagccc gcgaacgcgc ccaccgcgag
     1981 gcgctcgagc gcggcgttgg cgtcggcgag cgggaacgtc gtgacggcgg gatggagggg
     2041 gatcagggac gcctccatca gcagctcgcg gccatcctcg cgcgtgttgg cggtcacgct
     2101 ggtgacgcgg cgctcgtgga acaggtccgc atactcgacg ggagggatcc ggctcatgtg
     2161 gatgccggcc agcgccaggg tgccgccggg ggccagggcc cgcagggcca gcggcaccag
     2221 ctcgccggcc ggcgcgaaga tgatcgcgcc ctccaccggc gccggcgcgc ggtcgccgag
     2281 ctcgcccacc catgccgcgc cgagcgatcg cgccagcgcg cggtgggtgg cctcccgagt
     2341 gaagacgtac acctcgcatc cccgccgcgc ggcgatctgc agggtgatgt gcgcggacga
     2401 cccgaacccg tacatcgcca gacgctcacc cctcccgacc ccgctgagcc gcagggcgcg
     2461 gtagccgatg atcccggcac acagcagcgg ggccgcctcc acgtcgccga agatctccgg
     2521 gatcgggtac gcgaagtccg cgggaacgac cgcgtactcg gcataaccac catcggcgtc
     2581 ccagcccgtg aactccgccc gctcgcagag gttctcgcgc cgcttctcgc agaagcggca
     2641 ggcgccgcag gtgcgccgca gccaggcgat ccccacgcgg cccccgacgc ggggcgtgac
     2701 gacggcggcg cccaggcgct cgacatgtcc gacgacctgg tgccccggca cgaccggcga
     2761 gcggtgcggc ggcagctcgc cctcgatcac gtgcaggtcg gtgcggcaga tgccgcacgc
     2821 ggtcacgcgg acgagcacct cgccagcggc gggctcgggg atggccagcc tgcgctcccg
     2881 tagcgggcgc gtggtgatgg gcgccggcgc ctcgagcacc atggcgcgca tcgcgcgtcc
     2941 gggcgatgcg gcaggcgtca cgccccgggc cgcgtcggag gcgtctcgcc ggcgcgcgcg
     3001 aggacgcgga gcgcgcccgc caggtgcggg gcgtccacca tgcggccctt gaacgtgaag
     3061 gcgtacacgc cgcggcggcg gtgctcctcg aaggccgcgc agagctcgcg ggcctccgcg
     3121 acggcctccg gcgcgggcgt gaaggcggcg ttgatcacgg cgatctggct ggggtggatc
     3181 gagatcttgc cggtgaagcc catgtccacg ccgtcctggc actcccgccg gagcccgtcg
     3241 aggtccgcga tgtcggtgta gaccgtgtcg atcgactgca cgccggcggc cgcggcggcg
     3301 accgcgcaca tgacgcgtgc gtggcgcggg atgtccaggt actggccgcg ctcgtctcgc
     3361 acgcggccga ggcccatcgc cgcgccgagg tcctcgatgc cccaggacac cgcgacgatt
     3421 cgcgggctcg ccgcggcaac ctccttgatg ttgagcagcc cctcgggcgt ctcggtggcg
     3481 atgaggacga gccgggtggt gccgtggggg atcccgtgct cgtgctcgag gcggtcgagc
     3541 gcctgcgcga tctccctgac gtcggcagcc gaacgcggct tcggcaccac gtagccgtcg
     3601 ggccgcccgg cgatcgtctc ctcgagatcg gcgcgcccca ggcccgtcgc caccgggttc
     3661 atccgcaccc agcgctcgcg gccgccgaaa tcgagcttct cgagccagcc gcgcacgatg
     3721 ggccgcgtcg ccgccttgcg gtcgggcgcc accgcgtcct cgaggtcgag gatgaggccg
     3781 tcggcgggca gcgtcagcgc gcgggcgatc atccgctcgt tgccgccggg cacgaagtgc
     3841 aaagagcgcc gcggccgggg ggccgtcatc gcccgcctcc ctccccggcg cgcctgcgca
     3901 tcatcgcgtt gcgccgcgcc cgcatgacca cgtcaccgtg ctggttcacc gcgcggtgct
     3961 cgaaggtgac gatgccccac tgcggtcgcg atctggactc ccgcttgtcc acgacctcgg
     4021 tctccgcgta gatcgtgtcg ccgtggaaga cgggcttcgg gaactcgacc ttctcgaagc
     4081 ccagattgcc gacggtagtg ccgagcgtgg tctcgcccac cgagagcccg acggcgatgc
     4141 cgagcgtgag caggctgttc accagcggct tgccgaactc cgccttcgac gccgcgtgga
     4201 agtccacgtg caggggctgc gggttcatcg tcaggcacga gaagaacacg ttgtcggcct
     4261 cggtgatggt gcgccccggc tgatgccggt acgtctgccc gacctccagc tcctcgaagt
     4321 gcttccccgc catctccacg cctccccagc gtcccgttct cgccagaagt tcctgcgcga
     4381 cgtcgcgacc tcggggcggc tggacccgag tcggggcgag gtggggtccg tggacccgtt
     4441 cccgccgccg cgcgagaggg ctgtcgcggc accggatggt ataccaccac cgggagtgct
     4501 tgacacggct gggcgacgcc cctatgaatt ggtcgccatg cccgagcgct acgacgtcgc
     4561 gatcgtcggc ggcggcatcg tcgccctggc cactgcccgc gcgctgaacg agcgcgcccc
     4621 gcgggcccgg ctggccatcc tggagaagga agcggcgctg gcccagcacc agacgggcca
     4681 caacagcggg gtcatccact ccggcatcta ctacaagccc ggctcgtaca aggcgcggct
     4741 gtgcgtcgag ggcgcgcagc tcatgaagga gttctgcgag gcccatggta tccgcgtgga
     4801 ccgctgcggc aaggtcatcg tggccacgag cgccgaggag gtgccacggc tcatgacact
     4861 ctgggagcgg ggcaacgcca acggcgtgcc cggcttggcg ctcatcgacc cgccgcgcct
     4921 gcgggagctc gagccgaaca ccgtggcgct caaggccatc cactcgccga acacggcgat
     4981 cgtggacttc aaggaggtcg cggtggccct cgcgcggggg ctgacggcgg ccggcgtgac
     5041 catcgagacc gacgcccggg tgacagccat ccgtcgcgac ggtgaaggcc tcgagctgac
     5101 gacgccgcgc gctacggtac acgcccgtgg gctcgtgaac tgtgcgggtc tctactcgga
     5161 cgacgtggtg aggatggcgg gcgcggagcc cggcgtcagg atcgtgccgt tccgcggcga
     5221 gtactatttc ctgaagcccg agcggcgctc gctggtccgc gggctgatct acccggtgcc
     5281 cgatcccgag ttcccgttcc tcggcgtgca cttcacgaag acggtgcacg gcgacgtcga
     5341 ggccggcccc aacgcggtgc tggccttcgc gcgggagggc tacaccttcg ggcgcgtgcg
     5401 accgtcggag ctcctcgaca cgctccgcta cccgggcttc tgggtgatgg cgcgccgcta
     5461 ctggcgcacc ggcgcctacg aggtgtaccg ctcgctcagc aaggcggcgt tcgtgcgcgc
     5521 cctccagcgc ctcgtgcccg ccgtccgggg cgacgatctc tcgcgcggag gggcgggcgt
     5581 gcgcgcccag gccgtgaccc ccgagggcgc ccttgtggac gacttccgga tcgtcgagac
     5641 gaccggcgcc gtccacgttc tcaacgcccc ctccccggcg gcgaccgcct cgctcgccat
     5701 cggccggcac atcgcctccg tggcctcgga gcgcttcggc ctgaagtaag accttcgcga
     5761 ggtcctgcat ttgcccacag aggtctgtgg atagcggtgg aaacagtctg tagtggtccg
     5821 aggtggcacg gcgacaatag gtggtgtggt agccaaaaat ccctctattg acaggtactt
     5881 gtggctcgcg gtagcctatt ctacccatga ttaccgtgcc tgcgcgccgc gtgaagcagt
     5941 tcggtgtcga gttctaccag gctgggcttt ccgccaagga catcgaccgg ctcgtgaagt
     6001 tcgaggtcct gggctactcc ggcggtccgg gcgaggagcc caccaagggg cggagcaagt
     6061 cgcgctcgcg agtcaactgg gacgctctcg agaagcgcat cagcgagagc gagacggcgt
     6121 accagcggcc ggtcatccgc cgcaagatcg acgagctggt ggcgtactac cgcgagtgca
     6181 aggacgcggg cacgctgcca ccgatccccg gtgcggtcat catcacctcg gagcggcggt
     6241 tcacgttcac gccggtggct ggccagcacg acgtcgggct cctgca
//
LOCUS       DATIAZ010000142         5359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_430075, whole genome shotgun sequence.
ACCESSION   DATIAZ010000142 DATIAZ010000000
VERSION     DATIAZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430075"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VLF19_04995"
     CDS             <1..825
                     /locus_tag="VLF19_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HSE92642.1"
                     /translation="RGRKRVPSYVCAGYREKGKTFCTNQTGVPMAQLHKAVIAALKVT
                     FSDEKFEQYLRDQAADTEQIAARRAERENLLLQIPKLAAAEAKLAKAIAVSDDMDALV
                     AELKATQQERRQAEARVADLEGYERDLRDQQEQVERLRRVWGGWAAALDADVPTARQI
                     LKKILVGRIYVGPVRPGAWRFAGISRYDGALRGGLVRTAPADVLPGYGPRNDDVCRLM
                     LHLLAEPNGTAEALDPSRRESLFGLCTERRPAPAIAGGIDAGVWDQTGRVSSGPPM"
     gene            complement(826..1815)
                     /locus_tag="VLF19_05000"
     CDS             complement(826..1815)
                     /locus_tag="VLF19_05000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSE92643.1"
                     /translation="MLELELPTRHDWAVSDSTYGKLVIEPFEPGFGLTVGNAYRRALL
                     SAIHGAAPTWVKIENVLHEFSHLPGVMEDTLDILMNLRKVVFRLHVNRPKILRLKAQG
                     VGVVKAGDFEADADVEILTPDQPLATLDKDGMFEMEVCVERGRGYVPAERREPEALPI
                     NAILLDADFSPIKRVNFQVEPAGERERLVLEVWTDGSVAPTVAVGEASRIIEDHFDLL
                     MHFPETAPETEEAERDETRPRAELNENLFRNVDELELSVRASNCLKTANIRTIADLVQ
                     KSESELLKTKNFGKKSLNEIKTILGEMGLHLGMRLEPEELERLRSQFERNYET"
     gene            1910..2908
                     /gene="mltG"
                     /locus_tag="VLF19_05005"
     CDS             1910..2908
                     /gene="mltG"
                     /locus_tag="VLF19_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSE92644.1"
                     /translation="MRSRRLVVLLVVLAGLLGWQAWRTLTPAAALREGPVIVLVPANQ
                     GVLAVANHLHEVGVIGSPEAFVAMSVARGTFRTLKAGEYEMPRDASTADVLALLESGK
                     VRQRPVLHREGATIAELARSLEQAGLARAADVVRVAAEPAFLRAHGIEAPSAEGYLFP
                     DTYHFVLGMTPEQMLGRMVQRLHTKLTPDVRERARASGLGVHGLLTLASIIEREAVAD
                     DERPIISAVFWNRLRLGMPLQADPTVQYAVGKERRALTRADLTADHPYNTYQRSGLPP
                     GPIASPGWSAVEAALDPAAVKYLYFVKKDDERHHFSVSIDEHNSAVTRYRMARNRS"
     gene            3011..3679
                     /locus_tag="VLF19_05010"
     CDS             3011..3679
                     /locus_tag="VLF19_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSE92645.1"
                     /translation="MAPSAAVTVKPRAAAGGATGPLLYRLLRAPVRAALERFFDLRVD
                     GLAHVPPRGPYIVAANHHNYLDGVVLAVTAPAPVGFLVMPRVWNATPLHPLLHRHLGS
                     IPLRLGRPDAGALRAALAALQDGRVVGIFPEGPFSVRGRLEPGLPGVALLALRSGVPV
                     VPAGIRGTFEALVGRRFHVPRRHPLRVRFGAPRRFTARGDAARATRLAVTDRIMADIA
                     DLLA"
     gene            3676..5055
                     /gene="argH"
                     /locus_tag="VLF19_05015"
     CDS             3676..5055
                     /gene="argH"
                     /locus_tag="VLF19_05015"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSE92646.1"
                     /translation="MKKAWSGRFTQAADPTAERFTASLAFDRRLALHDVAGSIAWARA
                     LARAGLVTDGERDAIVEGLEAVRAELEGGTFPYRPELEDIHINVERRLVELCGPVGGK
                     LHTGRSRNDQIALDERLYLRDVAAAVDEGLRQTQAALIAQAERGVDAPMPGYTHLQRA
                     QPVVLAHHLLAYVFMLQRDRERFADARARADVLPLGSGALAGAGFAIDREALARDLGF
                     AAASPNSLDAVSDRDYLLEFLAAAAITGMHLSRLAADLTLWATAEFGFVEFSDAFATG
                     SSMMPQKKNPDVAELIRGKSGRLYGNLVAVLTTMKGLPLTYNSDMQEDKEPFFDSVDT
                     LAAILAVLPPMLASLTFQTERMRRAAGEHFATATDLADYLVRRGVPFREAHEVVGRVV
                     RHALGAGKPLEALAVEELRGFSPLFGDDVHAAITVDASLRARAATGGTAPEAVRRALA
                     LARTLVAPR"
     gene            5052..>5359
                     /locus_tag="VLF19_05020"
     CDS             5052..>5359
                     /locus_tag="VLF19_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92647.1"
                     /translation="MTRRAAPAVLAALALVACGRVGAPVPPEVRLPRPVADLAASVGD
                     GAIQLTWSNPTHRADNTRLRELGVARVFRHESTEGTEPKPAMLSRGRIVGYTEIGTIP
                     L"
BASE COUNT          721 a         1976 c         1896 g          766 t
ORIGIN      
        1 cgcggtcgga agagggtccc cagctacgtg tgcgccggct accgcgagaa gggcaagacc
       61 ttctgcacca accagaccgg cgtgccgatg gctcagctgc acaaggcggt catcgcggca
      121 ctgaaggtca cgttctcgga cgagaagttc gagcagtacc tccgcgacca ggcggccgac
      181 accgagcaga tcgccgcccg ccgcgccgag cgcgagaacc tgctcctcca gatcccgaag
      241 ctggccgccg ccgaggccaa gctggccaag gcgatcgccg tgagcgatga catggacgcc
      301 ctggtcgccg agctgaaggc gacccagcag gagcgccgac aggccgaggc gcgcgtggcc
      361 gatctggagg gctacgagcg cgacctccgc gaccagcagg aacaggtcga gcggctacgt
      421 cgggtgtggg gcggctgggc ggccgcgctg gacgctgacg tgccgacggc caggcagatc
      481 ctcaagaaga tcctggtcgg ccggatctac gtcgggcccg tccgcccggg cgcctggcgg
      541 ttcgccggga tcagccggta cgatggcgcc ctgcggggcg gcctggtgcg cacagcgccg
      601 gctgatgtcc tgcccggcta cggcccccgg aacgatgacg tctgccggct gatgctgcac
      661 ctgctggccg agcccaacgg cacggccgag gccctggacc cgagccgacg ggagtcactg
      721 ttcgggctct gcaccgagcg caggccggcc ccggcgatcg ctggtgggat cgatgccggg
      781 gtctgggatc aaaccggtcg ggtcagctcc ggccccccca tgtaatcaag tctcgtagtt
      841 gcgctcgaac tgcgagcgca ggcgctccag ctcctcgggc tcgagccgca tccccaggtg
      901 caggcccatt tcgccgagga tcgtcttgat ctcgttcagc gacttcttgc cgaagttctt
      961 ggtcttgagc agctcggact cgctcttctg cacgaggtcc gcgatggtgc ggatgttcgc
     1021 ggtcttgaga cagttcgagg cgcggacgga cagctcgagc tcgtccacgt tccggaagag
     1081 gttctcgttc agctcggccc gcggccgcgt ctcgtcccgc tcggcctcct cggtctcggg
     1141 cgcggtctcg gggaagtgca tgaggagatc gaagtgatcc tcgatgatcc gcgaggcctc
     1201 gcccaccgcg acggtgggcg ccacgctgcc gtccgtccac acctcgagca ccaggcgctc
     1261 gcgctccccg gccggctcga cctggaagtt cacgcgcttg atgggcgaga agtcggcgtc
     1321 gagcaggatc gcgttgatgg gcagcgcctc gggctcccgg cgctcggccg gcacgtagcc
     1381 ccggccgcgc tcgacgcaga cctccatctc gaacatcccg tccttgtcca gcgtggcgag
     1441 cggctggtcg ggcgtgagga tctccacgtc ggcgtcggcc tcgaagtcgc cggccttcac
     1501 gacgccgacg ccctgcgcct tgagacggag gatcttgggc cggttgacgt ggagacggaa
     1561 cacgaccttt cggaggttca tgaggatgtc gagcgtgtct tccatcaccc cgggcagatg
     1621 ggagaactcg tggaggacgt tctcgatctt cacccaggtc ggcgcggcgc cgtggatggc
     1681 ggacaggagg gcgcgccggt aggcgttgcc caccgtgaga ccgaagccgg gctcgaaggg
     1741 ctcgatgacc agcttgccgt aagtgctgtc ggagacggcc caatcgtgac gggtggggag
     1801 ctcgagctcc agcatgcaga cccccttcgg gaattccggg gcgacgcccc gaacgcgtag
     1861 tataccagag catcggtcgg cggcggtcgg gtgatagact cgaagcatcg tgagatcgcg
     1921 tcgcctcgtg gtcctgctcg tcgtgctggc cgggctcctg ggctggcaag cgtggcgcac
     1981 gctgacgccc gcggcggccc tccgcgaggg gccggtgatc gttctcgttc ccgccaacca
     2041 gggcgtcctc gccgtggcca accacctcca cgaggtcggc gtcatcggca gccccgaggc
     2101 gttcgtcgcg atgagcgtcg cgcgcggcac cttccggacc ctcaaggccg gcgagtacga
     2161 gatgccccgc gacgcctcga cggccgacgt gctggccctg ctcgagagcg gcaaggtgcg
     2221 ccagcgcccg gtgctgcacc gcgagggcgc gaccatcgcc gagctcgcgc gctcgctcga
     2281 gcaggcgggc ctggcccgcg ccgccgacgt cgtccgcgtg gccgccgagc cggccttcct
     2341 ccgcgcgcac ggcatcgagg cgcccagcgc cgagggttac ctgttccccg acacgtatca
     2401 cttcgtgctg gggatgacgc ccgagcagat gctcgggcgc atggtgcagc ggttgcacac
     2461 caagctcacc cccgacgtgc gcgagcgggc gcgcgccagt ggcctgggcg ttcacggcct
     2521 gctcacgctg gcctcgatca tcgagcgcga ggcggtcgcc gacgacgagc ggccgatcat
     2581 ctcggcggtg ttctggaacc gcctgcgtct gggcatgccg ctgcaggccg atccgacggt
     2641 gcagtacgcg gtcggcaagg agcgtcgcgc gctcacgcga gcggacctga cggccgatca
     2701 tccttacaac acctaccagc ggagcggcct gccgcccggg cccatcgcga gccccgggtg
     2761 gagcgcggtc gaagccgcgc tcgatccggc cgccgtcaag tacctctact tcgtcaagaa
     2821 ggacgacgag cggcaccact tctccgtgag catcgacgag cacaacagcg cggtgacacg
     2881 ctaccgcatg gcgcgcaacc gcagctgaca tcggaagggg cgtcctgctg attcgccgcg
     2941 tgttcggtcg ccgggcgggg agggcctcgc gctaggcacg tcctggcagg cgtgctataa
     3001 caccggcgac atggcgccgt cggcggcggt caccgtgaag ccgcgcgccg cggcgggcgg
     3061 cgccaccggg cccctgctct atcggctgct gcgcgcgccc gtgcgcgccg ccctcgagcg
     3121 cttcttcgac ctgcgcgtcg atgggctcgc gcacgtgccg ccgcgcggcc cctacatcgt
     3181 cgccgcgaac caccacaact acctggacgg cgtcgtgctc gccgtcacgg cgcccgcgcc
     3241 ggtcggcttc ctggtgatgc cgcgagtgtg gaacgcgacg ccgctgcacc cgctcctgca
     3301 ccggcacctc ggctcgatcc cgctgcgcct gggccgtccc gacgcgggtg cgctgcgcgc
     3361 ggccctcgcc gcgctccagg acgggcgcgt cgtgggcatc tttcccgagg gcccgttcag
     3421 cgtgcgcggc cggctcgagc ccggcctgcc cggcgtggcg ctgctcgcgc tcaggtccgg
     3481 cgtgcccgtc gtgccggcgg gcatccgcgg caccttcgag gcgctcgtcg gacgccgctt
     3541 ccacgtgccg cggcgtcatc cgctcagggt ccgcttcggc gcgccgcgac ggttcaccgc
     3601 ccgtggcgac gccgcgcgcg cgacgcggct cgcggtcacc gaccggatca tggcggacat
     3661 cgccgacctg ctggcgtgaa gaaggcctgg tccgggcgct tcacgcaggc cgccgatccg
     3721 accgccgagc ggttcaccgc ctcgctggcc ttcgaccgcc ggctggcgct ccacgacgtc
     3781 gccggcagca tcgcctgggc ccgcgcactc gcgcgggccg ggctcgtgac cgacggcgag
     3841 cgcgacgcga tcgtcgaggg gctggaggcc gtccgcgccg agctcgaggg cggcacgttc
     3901 ccctaccgcc cggagctgga ggacatccac atcaacgtgg agcggcggct cgtggagctc
     3961 tgcggcccgg tgggcggcaa gctccacacc gggcgctcgc gcaacgacca gatcgcgctc
     4021 gacgagcggc tctacctgcg cgacgtcgcc gccgcggtgg acgaggggct gcgccagacc
     4081 caggccgcgc tcatcgcgca ggccgagcgg ggcgtggacg cgcccatgcc cggctacacg
     4141 cacctgcagc gcgcgcagcc cgtcgtgctc gcgcaccacc tgctcgccta cgtgttcatg
     4201 ctccagcgcg atcgcgagcg cttcgccgac gcccgggcgc gggccgacgt gctgccgctc
     4261 ggcagcggcg cgctcgccgg cgccgggttc gccatcgacc gcgaggcgct cgccagggat
     4321 ctcggcttcg ccgcggcgag cccgaacagc ctggacgccg tcagcgaccg cgactacctc
     4381 ctcgagttcc tcgccgccgc ggccatcacg ggcatgcacc tgagccggct ggccgccgac
     4441 ctcacgctgt gggccacggc ggagttcggc ttcgtggagt tctccgacgc cttcgccacc
     4501 ggctcgtcga tgatgccgca gaagaagaac cccgacgtgg cggagctgat ccgcggcaag
     4561 agcggccgtc tctacggcaa tctcgtcgcg gtcctgacga cgatgaaggg cttgcccctc
     4621 acctacaact ccgacatgca ggaggacaag gagcccttct tcgactcggt ggacacgctc
     4681 gccgcgatcc tcgccgtgct gccgccgatg ctcgcctcgc tgaccttcca gaccgagcgc
     4741 atgcgccggg ccgcgggcga gcacttcgcc accgccaccg acctcgccga ctatctcgtc
     4801 cgccggggcg tcccgttccg cgaggctcac gaggtggtgg gccgcgtggt ccggcacgcg
     4861 ctcggcgccg gcaagccgct ggaggcgctc gccgtggagg agctgcgcgg cttctcgccg
     4921 ctcttcggcg acgacgtgca cgcggccatc accgtggacg cgtcgctgcg cgcgcgcgcg
     4981 gcgacggggg gcaccgcgcc ggaggccgtc cgccgggcgc tcgcgctcgc tcgcacgctg
     5041 gtcgcccccc ggtgacccgt cgcgcggcgc ccgcggtcct cgccgcgctg gccctcgtcg
     5101 cgtgcggcag ggtcggggcg ccggtgccgc ccgaggtacg cctgccccgt cccgtcgccg
     5161 acctcgcggc ctccgtgggt gacggggcga tccagctcac gtggagcaac ccgacccacc
     5221 gcgcggacaa cacgcgactg cgggaactcg gcgtggcgcg cgtgttccgt cacgagagca
     5281 cggagggcac tgaacccaag ccggcgatgc tctcgcgcgg ccgcatcgtc ggctacacgg
     5341 agatcggcac gatcccgct
//
LOCUS       DATIAZ010000143         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_430160, whole genome shotgun sequence.
ACCESSION   DATIAZ010000143 DATIAZ010000000
VERSION     DATIAZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430160"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VLF19_05025"
     CDS             complement(<1..419)
                     /locus_tag="VLF19_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSE92648.1"
                     /translation="MKRATTGLFFATGVLAVIAAGAQPGDRVAFDGAAALRHVAALVA
                     LGPRPAGSPASARARDYIVRELRRAGVEARVERFDTDTPHGRLAMANVVGIVRGRRPD
                     TILVGGHYDTKWFKELPFVGANDGGSSTALLLELARRL"
     gene            complement(416..1267)
                     /locus_tag="VLF19_05030"
     CDS             complement(416..1267)
                     /locus_tag="VLF19_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE92649.1"
                     /translation="MTASHPDPFREIDAQADPARFLAALEERGRTPAQARLRRRFLRF
                     VPVRAGDRVLEVGCGSGVVLRDLAARVGPRGRVVGVDPSRWLLAAAARLLEGHALRGR
                     IALRTAAGARLPFAAGRFDVTVAVTVLLHVADPLAVVQEMARVTRPGGLVAVQDQDFG
                     TVALAHPDRALTERILDGVAAHIYEEPWSGRRLPGLLRAAGLTSIRLLTDVYQDTALV
                     PYTKAFIERRAENAVRFGIVDGRTAQAWLDALTALVARGDFVLTVNYYGAVGVRPGGP
                     NRRPRAR"
     gene            complement(1283..1522)
                     /locus_tag="VLF19_05035"
     CDS             complement(1283..1522)
                     /locus_tag="VLF19_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSE92650.1"
                     /translation="MRGLWILLWLALVVVLLVPLLRRLGTAAQRPRRELADELVKDPV
                     CQTYIARSRAVRGEAQGAPVYFCSETCARRHAASG"
     gene            complement(1529..1759)
                     /locus_tag="VLF19_05040"
     CDS             complement(1529..1759)
                     /locus_tag="VLF19_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92651.1"
                     /translation="MSREPHVDALMKKLLRTIEVALAATDAARDAVQEILKRGADTGI
                     FFAGSGRRGNAGFELTPQDKEFLRAVSIRPDR"
     gene            complement(1890..>2030)
                     /locus_tag="VLF19_05045"
     CDS             complement(1890..>2030)
                     /locus_tag="VLF19_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE92652.1"
                     /translation="MLVAKIPMGRVGTPEEVAALVAWLASDECSFSTGAVYDLSGGRA
                     TY"
BASE COUNT          290 a          748 c          755 g          237 t
ORIGIN      
        1 agacgccggg ccagctcgag caacagggcc gtgctggagc cgccgtcgtt ggcgccgacg
       61 aacggcagct ccttgaacca cttggtgtcg tagtggccac cgacgaggat ggtgtcgggc
      121 cgccggccgc gcacgatgcc caccacgttg gccatggcca ggcgtccgtg cggcgtgtcc
      181 gtgtcgaacc gctccacgcg cgcctccacg cccgcgcgcc ggagctcccg cacgatgtag
      241 tcgcgcgcgc gcgcggaggc cggcgatccg gccggcctgg gcccgagggc caccagcgcg
      301 gcgacgtggc gcagcgccgc cgcgccgtcg aaggccaccc ggtcccccgg ttgcgcgccg
      361 gccgcgatga cggcgagcac gccggtggcg aagaagaggc cggtcgtggc gcgcttcacc
      421 gggcgcgtgg ccggcggttg ggcccgcccg gccggacgcc gacggcaccg tagtagttca
      481 ccgtcagcac gaagtcccct cgggcgacga gcgcggtcag agcgtcgagc cacgcctgcg
      541 ccgttcgccc gtcgacgatc ccgaagcgga cggcgttctc ggcacgccgc tcgatgaagg
      601 cctttgtgta cgggacgagg gcggtgtcct ggtagacgtc ggtgaggagc cggatcgagg
      661 tgagcccggc ggcgcgcagg agtcccggca gccgccgccc gctccacggc tcctcgtaga
      721 tgtgcgcggc aacgccatcg aggatacgct cggtgagcgc gcggtcggga tgcgccagcg
      781 cgacggtgcc gaagtcctgg tcctggacgg cgaccagtcc gccgggccga gtgacgcgtg
      841 ccatctcctg aacgacggcc agcgggtcgg caacgtggag caggacggtc accgcgaccg
      901 tcacgtcgaa gcgaccggcc gcgaacggca ggcgcgcgcc ggcggccgtc cgaagggcga
      961 tgcgcccgcg gagcgcgtgg ccctcgagca ggcgcgccgc cgcggcgagc agccaccggc
     1021 tcgggtccac gccgaccacc cgccctcgcg gcccgacgcg cgccgcgagg tcgcgcagga
     1081 ccacaccgct gccgcagccg acctcgagca cccggtcgcc cgcacggacc ggaacgaatc
     1141 tcaagaaccg gcgcctgagc cgcgcctggg ccggggtgcg gccacgctcc tcgagcgcgg
     1201 cgagaaagcg cgcgggatcg gcctgggcgt cgatctcccg gaacggatcc gggtgcgagg
     1261 cggtcatcgg agcgcgacgg gctcagccgg aggcggcgtg gcgccgtgcg cacgtctcgc
     1321 tgcagaagta cacgggtgcg ccctgcgcct cgccgcgcac ggcgcgcgag cgcgcgatgt
     1381 aggtctggca gacgggatcc ttcaccagct cgtcggcgag ctcgcgcctc gggcgttggg
     1441 ccgccgtccc gaggcgccgc agcaacggca ccaggagcac gaccacgagg gccaaccaga
     1501 ggaggatcca gagcccccgc atcgccgctc agcggtcggg gcggatcgag accgcgcgga
     1561 ggaactcctt gtcctgcggc gtcagctcga agcccgcatt gccccgccgc ccgctaccgg
     1621 cgaagaagat cccggtgtcg gcgccacgct tgagaatctc ctggacggca tcgcgcgcag
     1681 cgtcggtcgc cgccagcgcg acctcgatgg tcctcagcaa cttcttcatg agcgcgtcga
     1741 cgtgcggctc gcgggacatc gcgagctcta gcctaggacc cgtccccgcc gctgtcaaac
     1801 gccgtgtgct gatgcttcgt ccgcggcccg gcccgtcgcc ggccggagga ggtcaggtat
     1861 tcgagctcgc cgcgcccgcg gcgcgcggac tagtacgtcg cgcggccgcc ggagaggtcg
     1921 tagacggcgc cggtcgagaa cgagcattcg tcggaggcca gccaggcgac gagcgccgcc
     1981 acctcctcgg gagtgcccac gcgccccatc gggatcttgg cgacgagcat
//
LOCUS       DATIAZ010000144         3976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1219090, whole genome shotgun sequence.
ACCESSION   DATIAZ010000144 DATIAZ010000000
VERSION     DATIAZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219090"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VLF19_05050"
     CDS             <1..725
                     /locus_tag="VLF19_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSE92653.1"
                     /translation="VPAYASSAVLGSREAYAEEAARFKHQGWTAYKIHPPTDPAEDMA
                     VSEAVRRSVGDGYTLMLDPTWAYQYPEALRVGRAVEALGFHWYEDPLADDDLLNYVKL
                     KQHLSIPILATEYAPGGFTAYAPWIVQRATDYLRGDVAVKGGITPLVKGAHLAEAFHM
                     NFEIHHGGNSLNNVANLHVTMAIRNCEFFEVLLPAASQKYGLAEEIEVDTRGLVHAPG
                     GPGLGARIDFDLIERRKTAVLG"
     gene            797..1477
                     /locus_tag="VLF19_05055"
     CDS             797..1477
                     /locus_tag="VLF19_05055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSE92654.1"
                     /translation="MGQNITLQAEDGQKLGAYRADPAGKPRGGVVIIQEIFGVNAHIR
                     QVCDGFAADGYVAIAPALFDRVEPNIQLGYEQPDIVRGREIRGKIGWDTMLMDTRAAA
                     KVLLDEKLKTGVIGYCMGGSLAWLSATRIDGLSGAVGYYGGAVADFAGEQPRCPVLLH
                     FGETDASIPKEHWDKIKAAQPKVPMHIYPGAGHGFSCDHRASYHEPSHRLARQRTIDF
                     LRQNVGEP"
     gene            1593..3464
                     /locus_tag="VLF19_05060"
     CDS             1593..3464
                     /locus_tag="VLF19_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HSE92655.1"
                     /translation="MASGVTGKLLHVDLTNRQTRVEEVPETVMRTFLGGGALAAHILL
                     RDMAAGVDPLGPDNVLVFVTSVLNGLSLSGTNRYTAAAKSPLTGGYGESEAGGWWGPE
                     LRAAGWDGLVIRGRAGAPTYLWIKDQQVEFRDARPYWGKLTGDVQEGIERELGDKRVR
                     VLQTGVAGERLVRFSAIVNQLKHFHGRGGLGAVMGAKNLKAVVVRGSKPPAAVDKDGA
                     KRALAWFREHYDRSKDRFHQLGSSSAVLALEASGILPTRNFRDGSFEHAKAISGQTMR
                     DTILTDRGTCYACAVACKREVEVKDLGVTPKYGGPEYESLAASGSLCGVSDLPKLALI
                     NQLYAQYVLDSISAGAVIAFAMECYEHGIITKAMTGGIDLTWGNADAVVQMVHLIGRR
                     EGIGDVLAEGVKRAAATLGGGAERFAMHVKGQELPMHDPRGKQGLSLAYALSPTGADH
                     MEAPHDPLYAGFHPQGHPMGVLGLIEPLDPLVLDAKKVRAFYETQKVWSAYNSVGMCD
                     FVGAPLNVLEMQPFVDYINAVTGWNMSLYELLKVGERNNTLARVFNVREGFTPADDVL
                     PQRLHEGLGNGALKGKRVDPDEFLAARRTYYEMAGWDPDTGRPKAAKLAELGVEASA"
     gene            complement(3593..>3976)
                     /locus_tag="VLF19_05065"
     CDS             complement(3593..>3976)
                     /locus_tag="VLF19_05065"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HSE92656.1"
                     /translation="HGTMREVTCLDCGERAPMERALARVRAGEEDPPCRSCGGILKSA
                     TISFGQGLVAADLERAQRAARACDLMLAVGTKLSVWPVAGVVPTAKEAGARVVIVNAE
                     PTEMDALADAVLRGPISALLPRLVD"
BASE COUNT          653 a         1409 c         1387 g          527 t
ORIGIN      
        1 gcgtccccgc ctacgcgagc tccgccgtcc tcggatcgcg ggaggcgtac gccgaggagg
       61 cggcgcggtt caagcatcag gggtggaccg cctacaagat ccacccgccg accgaccccg
      121 cggaagacat ggccgtcagc gaggcggttc gccggtcggt cggggacggc tacaccctca
      181 tgctggaccc gacctgggcg taccagtatc ccgaggccct ccgcgtgggc cgcgccgtcg
      241 aggcgctcgg cttccactgg tacgaggacc cgctcgccga cgacgacctg ctgaactacg
      301 tgaagctcaa gcagcaccta tcgattccga tcctggccac ggagtacgcc ccgggcggct
      361 tcaccgcgta cgccccctgg atcgtccagc gagccacgga ctacctgcgg ggggacgtcg
      421 ccgtgaaggg cgggatcacg ccgctggtga agggcgcgca cctcgccgag gcgttccaca
      481 tgaacttcga gatccaccac ggcggcaact cgctcaacaa cgtggccaac ctgcacgtga
      541 ccatggccat ccgcaactgc gagttcttcg aggtgctgct gcccgccgcc tcccagaagt
      601 acgggctcgc cgaggagatc gaggtggaca cgcgcgggct cgtccacgcg cccggcggcc
      661 ccggcctggg cgcacgcatc gacttcgatc tgatcgagcg ccgcaagacc gcggtgctcg
      721 gctgagcggc cggcggatgc ccacgaaccg aacgaatccg gcgctgaacc ccccatccat
      781 ctagaggaga ccaacgatgg gccagaacat caccctccag gccgaggacg gccagaagct
      841 cggcgcctac cgggcggatc cggcggggaa gccccgcggc ggcgtcgtca tcatccagga
      901 gatcttcggc gtcaacgccc acatccggca ggtctgcgac ggcttcgccg ccgacggcta
      961 cgtggccatc gccccggcgc tgttcgaccg cgtggagccg aacatccagc tggggtacga
     1021 gcagccggac atcgtccgcg gccgcgagat ccggggcaag atcggctggg acacgatgct
     1081 catggacacg cgcgccgcgg cgaaggtgct tctggacgag aagctgaaga ccggtgtgat
     1141 cggctactgc atggggggct cgctggcctg gctcagcgcc acccgcatcg acggcctcag
     1201 cggggccgtc ggctactacg gcggcgccgt cgcggacttc gccggcgagc agccgcgctg
     1261 cccggtgctg ctccacttcg gcgagaccga cgcgtcgatc cccaaggagc actgggacaa
     1321 gatcaaggcg gcgcagccga aggtcccgat gcacatctac cccggcgcgg gccacggctt
     1381 cagctgcgat caccgcgcga gctatcacga accgagccac cgcctcgcgc ggcagcgcac
     1441 catcgacttt ctccggcaga acgtcggcga gccttgaaga atccggaggg gacctcgacg
     1501 gccccctccg ggatctcccc cggggagatt gcgccggcgg ggcggcgcgc gaggagtggt
     1561 cagctcaggc acgctgcgag aaggagatcg cgatggcatc aggagtcacc ggcaagctgc
     1621 tccacgtcga tctcaccaac cggcagacgc gggtggagga agtgcccgag accgtgatgc
     1681 ggacgtttct cgggggcggg gcgctggcgg cgcacatcct cctgcgagac atggccgccg
     1741 gcgtcgatcc gctcgggccc gacaacgtcc tcgtcttcgt gacgagcgtg ctcaacggcc
     1801 tttcgctctc cggaaccaac cgctacacgg ccgccgcgaa gtcgccgctc accggcggtt
     1861 acggcgagtc cgaggccggc ggctggtggg ggcccgagct ccgcgcggcg ggctgggacg
     1921 gcctggtgat ccgcgggcgc gccggggcgc ccacctacct gtggatcaag gaccagcagg
     1981 tcgagttccg cgacgcacgc ccgtactggg gcaagctcac cggcgacgtc caggagggca
     2041 tcgagcggga actgggcgac aagcgcgtgc gcgtgctgca gaccggtgtc gccggcgagc
     2101 ggctcgtacg cttctccgcc atcgtgaacc agctcaagca cttccacggg cgcggcgggc
     2161 tcggcgcggt gatgggcgcg aagaacttga aggccgtcgt cgtgcgcggc agcaagccgc
     2221 cggcggcggt ggacaaggac ggcgccaagc gggcgctcgc gtggttccgc gagcactacg
     2281 accggtcgaa ggaccgcttc caccagctcg gctcgtcgag cgcggtgctg gcgctggagg
     2341 cctccggcat cctgcccacc cgtaacttcc gcgacggctc gttcgagcac gccaaggcca
     2401 tcagcggcca gacgatgcgc gacaccatcc tcaccgaccg cggcacgtgc tacgcctgcg
     2461 cggtcgcgtg caagcgcgag gtcgaggtga aggacctggg agtgacgccc aagtacggcg
     2521 ggccggagta cgagagcctg gccgcctcgg ggtcgctgtg cggcgtcagc gacctcccga
     2581 agctcgcgct gatcaatcag ctctacgccc agtacgtgct cgactccatc tccgccggcg
     2641 cggtgatcgc gttcgccatg gagtgctacg agcacggcat cattacgaag gcgatgactg
     2701 gcggcatcga cctgacctgg ggcaacgccg acgcggtggt acagatggtg catctgatcg
     2761 gccgccgcga aggaatcggt gacgtcctcg ccgagggcgt caagcgggcg gcggccacgc
     2821 tcggcggcgg cgccgagcgg ttcgcgatgc acgtcaaggg ccaggagctg cccatgcacg
     2881 acccacgcgg caagcagggg ctctcgctcg cctacgcgct gtcgcccacc ggagccgacc
     2941 acatggaggc gccacacgac ccgctctacg ccgggttcca tccgcagggg cacccgatgg
     3001 gcgtgctcgg gctcatcgaa ccgctcgatc cgctggtgct cgacgccaag aaggtccgcg
     3061 cgttctacga gacccagaag gtgtggagcg cctacaactc ggtcggcatg tgcgacttcg
     3121 tcggcgcgcc cctcaacgtg ctggagatgc agccgttcgt ggactacatc aacgcggtga
     3181 ccggctggaa catgagcctc tacgagctgc tcaaggtcgg cgagcgcaac aacacgctgg
     3241 cacgggtgtt caacgtgcgg gagggcttca cgcccgccga cgacgtgctg ccgcagcgct
     3301 tgcacgaggg cctcggcaac ggcgcgctga agggcaagcg cgtcgatccc gacgagttcc
     3361 tggccgcgcg ccggacgtat tacgagatgg ccggctggga ccccgacacc ggtcggccga
     3421 aggcggcgaa gctcgccgaa ctgggcgtgg aggccagcgc gtaaacctcg tcctcatcga
     3481 ggaatcgcgg cacctgcgac catgaccgag gctcgccacg gctgtgccgg ggcgggccga
     3541 gccaccctgg ggggcttggg gggcgcccgg agccccccat gtaatcgatc cgtcaatcga
     3601 cgagtcgcgg cagcagcgcg ctgatcggac cgcgcagcac ggcgtcggcc agcgcatcca
     3661 tctcggtcgg ctccgcgttc acgatcacca cgcgggcgcc ggcctccttc gccgtcggca
     3721 ccacgcccgc caccggccac accgagagct tcgtgccgac ggcgagcatg aggtcgcagg
     3781 cgcgggccgc ccgctgggcg cgctcgaggt ccgcggccac gagaccctgg ccgaacgaga
     3841 tggtggccga cttcaagatc ccgccgcagg agcggcacgg cggatcctcc tcgccggcgc
     3901 ggacgcgggc gagcgcgcgc tccatcggcg cccgctcgcc gcagtcgaga cacgtcacct
     3961 cgcgcatcgt gccgtg
//
LOCUS       DATIAZ010000145         8172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_563543, whole genome shotgun sequence.
ACCESSION   DATIAZ010000145 DATIAZ010000000
VERSION     DATIAZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563543"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VLF19_05070"
     CDS             complement(<1..286)
                     /locus_tag="VLF19_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSE92657.1"
                     /translation="MRLYRLKRLFVGSPLPTAQQRHERLGKAAALAIFASDPLSSVAY
                     ATEEILIVLLLAGTVALSYSLPIGVGIAALIIIVISSYRQTIRAYPHGGGA"
     gene            complement(476..1435)
                     /locus_tag="VLF19_05075"
     CDS             complement(476..1435)
                     /locus_tag="VLF19_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004059467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HSE92658.1"
                     /translation="MGRKFRQLLKDEDYVFTGGVYTPLDAQIAERAGMKAIYMSGYSV
                     AMANGWPDMGFLTMTEVTRSASMMASAAEVPIIADADDGYGNALSTIRTVQEFVKTGV
                     AGIHLEDQRFPKRCGHIAGKTIVSREEAIGKFRAAIDTRDRLDRDFVIIARTDAYGAV
                     GGSLDEAIWRGRAYADAGVDLVWSEFSNAGREPAVAFAKAMRETHPALPLAFNYSSSF
                     RWSSDANPFLFRELGDLGYKFIFITLYAAHAATYAVWNAMEDLVKNQEQAQWTLERMK
                     AGHPTESHHVMARVAHFQELEKRYIPGSEDRIKSSHGFDDHRT"
     gene            complement(1662..1853)
                     /locus_tag="VLF19_05080"
     CDS             complement(1662..1853)
                     /locus_tag="VLF19_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92659.1"
                     /translation="MGGSCRCGNQISHEFHQLECIECGEPACPACAVPLESVTYCQGC
                     ADSLLGGTTVRAGGTFDLH"
     gene            complement(2064..3437)
                     /locus_tag="VLF19_05085"
     CDS             complement(2064..3437)
                     /locus_tag="VLF19_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSE92660.1"
                     /translation="MTKVVIVGAGKGGRALLEMFAGDPTVSILGIADLNPWAPGIDFA
                     RRLNVAVTTDYRELITDPRVDLIIDVTGNPDVQRAILRLKPATTEVMGGIGARFMWDL
                     LAERKRSEELEDRYSLVVRELAAQAEADFIIGQNPKMREVTELVARVAPTPTTVLIRG
                     ESGTGKELVARAIHKYSPLRDKPLLTVNCTALTPTLMESELFGHRRGAFTGALADKVG
                     LFEKADGGTIFLDEIGDMPLEMQGKMLRVLQAGEVKPVGDVVTRRVRVRVIAATNRDL
                     EAAIQRGEFREDLFYRFNTFTVTLPPLRERVEDIPVLAHHFLRKAEAKVNKKVDRISG
                     EALDLLARYAWPGNLRELENIVERAVVLAPERAIEPEHLPMHLQEPGAAALPLAADDG
                     LPAAKDRLLREFERQALVRFLREARGNVSIAARRAKITRRNFHRLLLKHKIDPSSFRG
                     VTRKTHV"
     gene            complement(3554..4924)
                     /gene="dnaB"
                     /locus_tag="VLF19_05090"
     CDS             complement(3554..4924)
                     /gene="dnaB"
                     /locus_tag="VLF19_05090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSE92661.1"
                     /translation="MSPLEIPSRIPPHNLDAERAVLGAVLLEGRETLPRVIEMLRSSD
                     FYTEAHRTIFETTLRLFDRAEPVDLITLSEELRRSDALEAVGGPAALALLVEHASIAA
                     HLTAYAGIVRDMAVLRELIQTSTQIIAQAFEAKEDVQTLVDDAERRIFGLAERRLEGS
                     ALPVGKILRNTFEYIERLYERKEHVTGVATGFEKLDLETSGLQPSDFVIIAGRPSMGK
                     TAFALNIAQHVGVALHGKVLVLSLEMSAPQLVQRMLCSEAKVDSQAVRTGRLTAADWH
                     RLTAAAGRLSEAAIFIDDSPGLTVLEARAKARRMKAEHGLDLVVIDYLQLMRGRASME
                     SRQQEISEISRSLKALAKELTVPVVALSQLSRAVESRATRDFRPQLSDLRESGALEQD
                     ADLILFLYRPSVYKEDLPPDEANITEVIIGKQRNGPVGTVKVVFLPQYARFENVADFH
                     RQPQPF"
     gene            complement(5152..5595)
                     /gene="rplI"
                     /locus_tag="VLF19_05095"
     CDS             complement(5152..5595)
                     /gene="rplI"
                     /locus_tag="VLF19_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSE92662.1"
                     /translation="MKVILLDDVAKVGRRGEVREVSDGYARNFLIPKKLALGATAGNL
                     KNLDHIKKQQEAKADRVKGDAEGLRARIEALIYEEPRQASEEGKLFGSVTSQDVVDFL
                     TSRGLKIERRRVHLDEPIKTLGETSVAIRLHPEVTAHLRVSVVRQ"
     gene            complement(5603..5851)
                     /gene="rpsR"
                     /locus_tag="VLF19_05100"
     CDS             complement(5603..5851)
                     /gene="rpsR"
                     /locus_tag="VLF19_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSE92663.1"
                     /translation="MPAPAKPVKRRRFGRRKVCKFCADRMDLVDYKDVRRLRNFISER
                     GKIIPRRISGSCARHQRQLTHAVKRARTVALLPYVSTD"
     gene            complement(5867..6286)
                     /locus_tag="VLF19_05105"
     CDS             complement(5867..6286)
                     /locus_tag="VLF19_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSE92664.1"
                     /translation="MASLNKVLLIGNLTRPPELRYTPSGTAVADLRLAVNRNYTVQSG
                     ERREETCFLTVVVWGKQAESCGEYLDKGSQVFVEGRLQTRDWEGKDGQKRTVTEVVAE
                     RVQFMSRTKGAAAAAAVPAASAFAEETTTGGEDDVPF"
     gene            complement(6286..6645)
                     /gene="rpsF"
                     /locus_tag="VLF19_05110"
     CDS             complement(6286..6645)
                     /gene="rpsF"
                     /locus_tag="VLF19_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSE92665.1"
                     /translation="MSSLRPYEILVIVDPRPTDEEVAALLTQLGEQVKGLGADVGNVD
                     NWGKRRLAYEIRKQREGTYAVFTVSAEPAMVKEFERQLRLNENVLRFLSTRVPIRKKV
                     RSPKAQPKSDEVVEEAV"
     gene            complement(6880..>8172)
                     /gene="groL"
                     /locus_tag="VLF19_05115"
     CDS             complement(6880..>8172)
                     /gene="groL"
                     /locus_tag="VLF19_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSE92666.1"
                     /translation="ANPMGLKRGIETAVDAVVEELKKLSKSTKDKKEISQVATIAANN
                     DKTIGGLIADAMEKVGKDGVITVEESKSADTVLDVVEGMQFDRGYLSPYFVTDAERME
                     CILEDALILIHEKKISVMKDMLPLLEQVARAGRPLLIIAEDVEGEALATLVVNKLRGT
                     LHCAAVKAPGFGDRRKAMLEDIAILTGGKAITEDLGIKLENIKLDDLGKAKKVKVDKD
                     NTTIVEGAGKQSGIEGRIKQIRAQIDETTSDYDREKLQERLAKLAGGVAVIKVGAATE
                     TAMKEKKARVEDSLNATRAAVEEGIVPGGGVALLRAAKAIDGMKLEGDEKTGASIVKR
                     ALEEPIRQIVENAGLEGSVVVEKVKAESMATRGFDAETMQPVDMFQAGIIDPTKVERV
                     ALQNAASIASLLLTTEALITDIPEEEKKAPPMPHGDMY"
BASE COUNT         1243 a         2799 c         2674 g         1456 t
ORIGIN      
        1 aggcgccgcc gccgtgcggg tacgcccgga tggtctgccg ataggagctg atgacgatga
       61 tgatgagggc cgcgataccc acgccgatcg gaagcgaata gctcagcgcc acggtgccgg
      121 ccagcaagag gacgatcagg atttcctcgg tcgcgtacgc caccgacgac aggggatcgg
      181 aagcgaagat cgccagcgcg gcggccttgc cgagacgctc gtgacgctgc tgcgcggtgg
      241 ggagaggcga gccgacgaag agccgcttca gacgatacag acgcacggcc accatcatac
      301 gccgccgcgt cggcggtggt cgtcaaacta accgccactc cacggccgcc agagaccggg
      361 ggcgccaacg gtcatcgggg ccatcgagca acacctcggc agctttagga gcatcgaggc
      421 gaggggctcg ggagccacgt cgggcctccc gtgccggtca atgcccccca ggcgctcacg
      481 tccggtggtc gtcgaacccg tgcgacgact tgatgcggtc ctcgctaccc gggatgtagc
      541 gcttctccag ctcctggaag tgcgcgacgc gggccatgac gtgatggctc tcggtcggat
      601 gcccggcctt catcctctcg agcgtccact gcgcctgttc ctggttcttg acgaggtctt
      661 ccatggcgtt ccacaccgca taggtggcgg cgtgcgccgc gtagagggtg atgaagatga
      721 acttgtagcc gaggtcgccc agctcgcgga agagaaacgg gttggcgtcg ctactccacc
      781 ggaacgacga cgagtagttg aaggccagcg gcagagcggg atgcgtctcg cgcatggcct
      841 tcgcgaacgc gacggccggc tcccggcccg cgttcgagaa ctccgaccag acgaggtcca
      901 cgccggcatc cgcgtaggcg cgcccgcgcc agatggcctc gtcgaggctc ccgccgaccg
      961 ccccgtacgc atccgtgcgc gcgatgatca cgaagtcgcg atcgaggcgg tcccgggtgt
     1021 cgatcgcggc gcggaacttc ccgatcgcct cctcgcggct gacgatcgtc ttgccggcga
     1081 tgtggccgca ccgcttcggg aaccgctggt cttcgaggtg gatgccggcc acgcccgtct
     1141 tgacgaactc ctggaccgtg cggatcgtgc tcagcgcgtt gccgtaaccg tcgtcggcgt
     1201 cggcgatgat cggcacctct gccgcgctcg ccatcatcga cgccgagcgg gtgacctcgg
     1261 tcatcgtgag gaagcccatg tccggccagc cgttcgccat cgcgacggaa tagccgctca
     1321 tgtagatcgc cttcatcccc gcgcgctcgg cgatctgggc gtcgagcggt gtgtagacgc
     1381 cgcccgtgaa gacgtagtcc tcgtccttca gcagctggcg gaatttcctt cccatgctgt
     1441 ccacggcttc agcgtcctcc tgtgtgccgg cgctcgggcc ggctcgtgcc cgcgccgctc
     1501 aggcggcagc ggtgacggcg caccaccggc agtaggtctg cgtcccgatg ggcagggcac
     1561 agctcggaca gcaagtggcc ccgcagtctt cacatccgat cggctcggtc tcgcgctcgt
     1621 gaagcagtcc acactcgcag gtcatgtagc ctccccgcgg cttagtggag gtcgaacgtg
     1681 ccgccagccc gcacggtggt gcctcccagc agagagtccg cgcagccctg gcagtacgtg
     1741 acggactcga ggggcacggc acaggccggg catgccggtt caccgcactc gatgcattcc
     1801 agctggtgga actcgtgact gatctggttc ccgcagcgac aactcccgcc catcactgtc
     1861 ctcctggacc agctttggtg ggatgtactt caagaggcgt tccagccaca agcttaggaa
     1921 aaatatgatg tattctttcc ggattcggtg gatgtgagtc ctgtatgtca catggtaaat
     1981 cgaccgcaaa ggagtgaatt tcatgccaat tagtagatat attcgtaact tatagtctag
     2041 ctaagtattg cgacttagat gtctcacaca tgagtcttgc gtgtcacacc gcggaaagag
     2101 cttggatcta tcttgtgttt gaggagaagg cggtgaaaat tcctccgcgt gatcttggcc
     2161 cgccgggctg caatggacac gttgccccgg gcctctcgga gaaacctcac gagggcctga
     2221 cgctcgaatt ctcggagcag gcggtccttc gccgccggca gcccgtcgtc cgcggccagc
     2281 ggcagggccg ccgccccggg ctcctggagg tgcatgggaa ggtgctcggg ctcgatggcg
     2341 cgctcggggg cgagaacgac cgcgcgctcg acgatgttct cgagctcgcg caggttgccc
     2401 ggccatgcgt agcgggccaa caggtcgagg gcctcgcccg agatgcggtc gaccttcttg
     2461 ttcaccttgg cctcggcctt gcgcaggaag tgatgggcga gtacgggaat gtcctcgacc
     2521 cgctcccgca gtggcggcag cgtgacggtg aacgtgttga agcggtagaa gaggtcttcc
     2581 cggaactcgc cgcgctggat ggccgcctcg aggtcgcggt tggtcgcagc gatcacgcgc
     2641 acgcgcaccc gccgcgtgac gacgtcaccc accggcttca cctcacccgc ctgaaggacg
     2701 cggagcatct tgccctgcat ctcgaggggc atgtcgccga tctcgtcgag gaatatcgtg
     2761 ccgccgtcgg ccttctcgaa cagccccacc ttgtcggcca gggcgccggt gaaggcgccc
     2821 cgccggtgac cgaacagctc ggactccatc agcgtgggcg tgagcgccgt gcagttgacg
     2881 gtcagcagcg gcttgtcccg gaggggcgag tacttgtgaa tcgcccgggc gacgagctcc
     2941 ttgccggtcc ccgactcgcc gcggatcagc acggtcgtcg gcgtcggtgc cacgcgcgcc
     3001 accagctcgg tcacctcgcg catcttcggg ttctggccga tgatgaagtc ggcctcggcc
     3061 tgggcggcca gctcgcgcac gaccagcgag taccggtcct ccagctcctc cgagcgcttc
     3121 cgttccgcga gcaggtccca catgaagcgg gcgccgatgc cgcccatcac ctcggtcgtc
     3181 gccggcttca gcctcaggat cgcccgctgc acgtcggggt tgccggtgac gtcgatgatc
     3241 aggtccactc gagggtcggt gatgagctcg cggtagtcgg tcgtgaccgc cacgttgagg
     3301 cgccgcgcga agtcgatacc gggcgcccac gggttgaggt cggcgatgcc gaggatggac
     3361 accgtcggat cgccggcgaa catctccagc agagcccgac cgcccttgcc ggcaccgacg
     3421 atcaccacct tcgtcacgcc ttagaccata tcatccggcg gcgtcacacg tttcgcgcgc
     3481 gggcttcgct cgcccctcct tccccgccga gtcagtggaa gggccgttga agcccccgtc
     3541 gaagccccca gcgctagaag ggctgcggct gtcggtggaa gtcggcgacg ttctcgaagc
     3601 gcgcgtattg cggtaggaaa accaccttca cggtcccgac cggcccgttc cgctgcttgc
     3661 cgatgatgac ctccgtgatg ttggcctcgt ccggcggcag gtcctccttg tagactgacg
     3721 gccggtacag gaaaaggatc aagtccgcgt cctgctcgag cgctccgctc tcacgaaggt
     3781 cgctgagctg tggccggaag tcgcgggtgg cgcgcgattc gaccgcgcgc gagagctgtg
     3841 acagcgccac aacggggacg gtcagctcct tggcgagggc cttgagcgag cgtgagatct
     3901 cggaaatctc ctgctggcgg ctctccatgg acgcgcgacc gcgcatgagc tgcaggtagt
     3961 cgatgacgac gaggtccagc ccgtgctcgg ccttcatccg ccgcgccttg gccctcgcct
     4021 cgagcacggt gaggccggga ctgtcgtcga tgaagatggc cgcctcgctg agccgcccgg
     4081 ccgcggcggt gagccggtgc cagtcggccg ccgtcaggcg cccggtgcgc accgcctggg
     4141 agtccacctt ggcctcggag cagagcatgc gctgcacgag ctgcggcgcc gacatctcga
     4201 ggctcaggac cagcaccttg ccgtggagtg ccacaccgac gtgctgcgcg atgttcaggg
     4261 cgaaggccgt cttccccatg ctgggccggc cggcgatgat cacgaagtcc gacggctgca
     4321 gcccggaggt ctccagatcg agcttctcga agcccgtcgc cacgccggtg acgtgctcct
     4381 tccgctcgta gagccgctcg atgtactcga acgtgttgcg gaggatcttg ccgacgggga
     4441 gcgcgctgcc ctcgagccgc cgttcggcca ggccaaagat gcggcgctcg gcgtcgtcca
     4501 ccagcgtctg cacgtcctcc ttggcctcga acgcctgggc gatgatctgc gtggacgtct
     4561 ggatcagctc acgcagcacc gccatgtcgc gaacgatccc ggcgtaggcg gtgaggtgcg
     4621 cggcgatgga cgcgtgctcg accagcaggg ccagcgccgc cgggccgccg acggcctcca
     4681 gggcgtcgct ccgcctcagc tcctcgctca gcgtgatgag atcgaccggc tcggcccggt
     4741 cgaagagccg cagcgtcgtc tcgaagatcg tgcgatgcgc ttccgtgtag aagtcggacg
     4801 agcggagcat ctcaatgacg cgcggcaggg tctcgcggcc ctcgaggagc accgcaccga
     4861 ggaccgcgcg ctcggcgtcc aggttgtggg gtgggatccg tgacggaatt tccagggggc
     4921 tcatgggcac gcctcagcgc gaagcatccg cgctgatcgt tgtagcgcgg ccgcgccatc
     4981 cggccaacga aaatcgtccg cgcgtcacgt cttgtggaca gggcgtgaac atccggtgga
     5041 gagtcgtgga ggaacggtgg acacggcgtg gacgacgggt ggataacgga ccgcggagag
     5101 gagactgcgc gaacgaaaga tccgtggacg ccgcgggccg gggccggcgc gctactggcg
     5161 gacgacgctc acgcggaggt gcgccgtgac ttcgggatgg agccggatgg ccacggacgt
     5221 ctcgccgagc gtcttgatcg gttcgtcgag gtgcacgcgc cgccgctcga tcttcaggcc
     5281 ccggctggtc aggaagtcga cgacgtcctg ggacgtcacc gacccgaaga gcttgccctc
     5341 ctcggacgcc tggcgaggct cctcgtagat cagggcctcg atgcgcgccc gaagcccttc
     5401 ggcgtcgccc ttgacgcggt cggccttggc ctcctgctgc ttcttgatgt ggtcgaggtt
     5461 cttcaggttg ccggcggtgg cgccgagcgc cagcttcttg gggatgagga agttccgtgc
     5521 gtaaccgtcc gacacctcac gaacctcgcc ccggcggccc accttcgcga cgtcgtcgag
     5581 caggatgacc ttcatcgctc tcctagtccg tggacacgta gggcagtaac gcgaccgtgc
     5641 gggcgcgctt caccgcgtgg gtgagctgac gctggtgacg ggcgcagctg cccgagatgc
     5701 ggcgcgggat gatcttgccg cgctcgctga tgaaattgcg gagccggcgg acgtccttgt
     5761 aatcgacgag gtccatccgg tccgcacaga acttgcagac cttccgccgt ccgaagcggc
     5821 ggcgcttgac tggcttcgct ggcgccggaa tagctcttcc tccttgttag aacggaacgt
     5881 cgtcctcgcc gccggtcgtc gtctcctcgg cgaaggccga cgccgccggc acagcggcgg
     5941 ccgcggcggc gcctttcgtg cggctcatga actggacgcg ctcggcgacg acttcggtca
     6001 ccgtgcgctt ctgcccgtcc ttgccctccc agtcgcgcgt ctgcagccgg ccctccacga
     6061 acacctgact gcccttgtcg agatactcac cgcagctctc ggcctgcttg ccccacacga
     6121 cgacggtgag gaagcacgtc tcctcccgcc gctcaccgct ctgcacggtg tagttccggt
     6181 tcaccgccag gcgcagatcg gccacggcgg tgccgctggg cgtgtaccgg agctcgggtg
     6241 ggcgcgtcag gttgccgatc agcaggacct tgttgaggct cgccatcaga ctgcctcctc
     6301 caccacctcg tcgctcttcg gctgagcctt cggggaccgg accttcttgc ggatgggcac
     6361 ccgggtcgac aggaagcgca ggacgttctc gttgagccgg agctgccgct cgaactcttt
     6421 gaccatcgca ggctcggcgg agaccgtgaa gacggcgtag gtgccttcac gctgcttccg
     6481 gatctcgtac gcgaggcggc gctttcccca gttgtcgacg ttgccgacgt cggctcccag
     6541 ccccttcacc tgctcaccga gctgcgtcag cagcgcggcc acctcttcgt cggtgggacg
     6601 ggggtcgacg atcaccagaa tttcgtacgg tcgcagactg ctcacctcct ctcgaagcgc
     6661 cttagcctaa cagaacacgc aacacgccgg caaggtcaac gcccaaaaag aaatacggcc
     6721 cgggccggtc gcccggccag ggccgccgcc cccgcatcgc gggagctcaa ctcgcacgtt
     6781 gcgtccgtcc gctcgctcgc cggcttcgcc ggcgcaatcc tcgggcacgg tacggggagg
     6841 ggccgtcgac ggcccctccc cgtgaaacct cctccgcgct caatacatat cgccgtgcgg
     6901 catcggcgga gccttcttct cctcctccgg aatgtccgtg atgagggctt ccgtcgtcag
     6961 gagcagcgag gcgatcgacg ccgcgttctg cagcgccacc cgctcgacct tcgtcgggtc
     7021 gatgatgccg gcctggaaca tgtcgaccgg ctgcatggtc tccgcatcga agccgcgcgt
     7081 cgccatgctc tcggccttca ccttctcgac cacgacgctc ccttcgagcc ccgcgttctc
     7141 gacgatctgg cgaatcggct cctccagcgc ccgcttgacg atcgaggcgc cagtcttctc
     7201 gtcaccttcg agcttcatgc catcgatggc cttcgccgcg cgcagcagcg ccacgccgcc
     7261 gccgggcacg atgccctcct cgacggccgc gcgggtggcg ttgagcgaat cctccacgcg
     7321 cgccttcttc tccttcatcg ccgtctcggt ggccgccccc accttgatga ctgccacgcc
     7381 gcccgccagc ttcgccagcc gctcctgcag cttctcgcgg tcgtagtcgg aggtcgtctc
     7441 gtcgatctgg gcgcggatct gcttgatgcg cccctcgatc ccggactgct tgccggcgcc
     7501 ctcgacgatc gtcgtgttgt ccttgtcgac cttcaccttc ttggccttgc cgaggtcgtc
     7561 gagcttgatg ttctcgagct tgatgccgag atcctcggtg atggccttgc cgccggtgag
     7621 gatggcgatg tcctcgagca tggccttacg gcggtcgccg aagcccggcg ccttcaccgc
     7681 ggcgcagtgc agcgtgccgc ggagcttgtt gaccacgagc gtggccagcg cctcaccctc
     7741 gacgtcctcg gcgatgatca acagcggccg acccgcgcgc gccacctgct cgagcagcgg
     7801 cagcatgtcc ttcatcacgc tgatcttctt ctcgtggatg aggatgagcg cgtcctccag
     7861 gatgcactcc atgcgctcgg cgtcggtgac gaagtacggc gagaggtagc cgcgatcgaa
     7921 ctgcatgccc tcgaccacgt ccaggacggt gtccgccgac ttcgactctt cgaccgtgat
     7981 cacgccgtcc ttgcccacct tctccatcgc atccgcgatc aggccgccga tcgtcttgtc
     8041 gttgttggcg gcgatggtgg cgacctgcga gatctccttc ttgtccttcg tcgacttcga
     8101 gagcttcttc agctcctcga cgacggcatc gacggccgtc tcgatgccac gcttcagccc
     8161 catggggttg gc
//
LOCUS       DATIAZ010000146         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1877313, whole genome shotgun sequence.
ACCESSION   DATIAZ010000146 DATIAZ010000000
VERSION     DATIAZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877313"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1513)
                     /locus_tag="VLF19_05120"
     CDS             complement(398..1513)
                     /locus_tag="VLF19_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSE92667.1"
                     /translation="MVDLADVCRRFAHDHVARHGARMLPSHRRAIADIIACRSAALGG
                     HLWRCEACRAEVYAYHSCRNRSCPRCHRTQTERWLAARTAELLDAPYFHITITVPAEL
                     RAALRANQRDGYALLMKAAAEAIVELARDPRYVGGQVAVLAVLHTWTQQLHYHPHVHC
                     LVSGGGISDDGRFWHPARKGFLVPVKALAKLVRGKLRDALARQRPDLQLPALAWNKPW
                     VVHATAWGAGPQAVLDYLARYLFRVAITNNRIVALDDQTVTIRYKKRKTNRPRTCRIE
                     GGEFMRRFLSHVLPKGLHKVRYFGLWHPSRRALVQRARLLLARERRAGPPPEPSPDAQ
                     SRPPAAEDEPRRPLCPCCRQGRLVHLRRLGPGNPLGP"
     gene            complement(1527..2126)
                     /locus_tag="VLF19_05125"
     CDS             complement(1527..2126)
                     /locus_tag="VLF19_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSE92668.1"
                     /translation="MAVVLKKNRIRLPKQKRLPESLSDGQVRRLLACVRSPKHRTCLQ
                     VMYGCGLRIGEAVKLQLDAVDREQEVLRIVAGKGGRDRRVSLPRPILEALRALWCTHR
                     HPRWLFPNRHGTGPLTADVVAATFRAAAADAGIGDHVTSHALRHSYATRLLEQGVDIR
                     VVQILLGHSNIASTAHYTHLSEPIRASVRQILDRIMRDL"
     gene            complement(2024..2374)
                     /locus_tag="VLF19_05130"
     CDS             complement(2024..2374)
                     /locus_tag="VLF19_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HSE92669.1"
                     /translation="MTPLRERMIGDMKLAGLSPRTQQAYTQAVKRLAMHYHRRPDRLD
                     EQEVRAFLVGVIDDGVARGTFKRVHFGIKFFFTTTLGRDWPLFSKKTASACRSRSVFP
                     SPSATARFAGCWLA"
     gene            complement(2499..2678)
                     /locus_tag="VLF19_05135"
                     /pseudo
     CDS             complement(2499..2678)
                     /locus_tag="VLF19_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
BASE COUNT          386 a          873 c         1075 g          419 t
ORIGIN      
        1 ttggcccgcg ccgtgttcct gatcacgccc ttggtgtggg gcgcgttagt gccggcgatc
       61 aggaacgaag taagagtttc tgcgtcgcgt agccgacgca gaggctcggt tggactggac
      121 gcgacttcgc ggcgaggcgg atgcttcggc tatggaattt tcaggctgac gggcggtggt
      181 gggagcccgg tcggcggctg gcacagcggt cggcagggtg cgccgaggcg atgcggcctc
      241 ggctcgggcc accttgaccc tgctcgtgac gggtcacgtg gccgggtcgg cggcgtctgc
      301 ggcagcgtgc ggcggtcgcg gcaacagcgg ggcggacgcc gacgcgtggc gacggcgcgg
      361 gatggtgccg gaaagctgct ttgtggttgt ggtcgggtca tggtccaagc gggttgccgg
      421 ggccgaggcg gcggaggtgg acgaggcggc cctgccggca gcaggggcag agggggcgtc
      481 gcggctcgtc ttcggcggcc ggcggccggg actgcgcgtc cggcgacggc tcggggggcg
      541 ggccggcgcg ccgctcgcgc gcgaggagca ggcgcgctcg ctggacgagc gcgcgtctgg
      601 acgggtgcca gaggccgaaa tagcggacct tgtgcaggcc cttgggcagc acgtggctga
      661 ggaaccggcg catgaactcg ccgccctcga tgcggcaggt gcgcggccgg ttggtcttgc
      721 gcttcttgta ccggatggtg accgtctgat cgtcgagggc gacgatgcgg ttgttggtga
      781 tggcgacgcg gaacaggtag cgcgcgaggt agtcgagcac ggcctgcggg cccgcgcccc
      841 aggcggtggc gtgcacgacc cagggcttgt tccaggcgag cgcgggcagc tgcaggtccg
      901 gccgctggcg tgccagggca tcgcggagct ttccgcgaac cagcttggcg agcgccttga
      961 ccggcaccag gaagcccttg cgggccgggt gccagaagcg gccgtcgtcc gatatgccac
     1021 cgccgctgac gaggcagtgg acgtgcgggt ggtagtgcag ctgctgggtc caggtgtgga
     1081 gcacagcgag caccgccacc tgcccgccga catagcgcgg gtcgcgggcg agctcgacga
     1141 tcgcctcggc ggccgccttc atcaagagcg catagccgtc acgttggttg gcgcgcagcg
     1201 ccgcccgcag ctccgcgggc acggtgatgg tgatgtggaa gtagggcgca tccagcagct
     1261 cggccgtacg cgcggcgagc cagcgttcgg tctgcgtgcg gtggcatcgg ggacagctcc
     1321 ggttgcggca ggaatggtag gcgtagacct cggcgcggca cgcctcgcag cgccagagat
     1381 ggccgcccag ggccgcgctg cggcaggcga tgatgtcggc gatggcgcgc cggtgcgagg
     1441 gcagcatgcg ggcgccgtgc cgcgcgacat ggtcgtgcgc gaagcgccgg cagacgtcgg
     1501 cgagatcgac catggcggcc tccgcatcag aggtcgcgca tgatccgatc gagaatctgc
     1561 ctgacgctgg cccggatggg ctcgctcaga tgggtgtagt gggcggtcga ggcgatgttg
     1621 ctatggccga gcaggatctg caccacgcgg atgtcgacgc cctgctcgag caggcgggtg
     1681 gcgtagctgt ggcggagcgc gtgcgaggtg acgtggtcgc cgatcccggc gtcggcggcc
     1741 gcggcgcgga aggtggcggc gaccacgtcg gcggtgagcg ggccggtgcc atgacggttg
     1801 gggaacagcc agcgcggatg ccggtgggtg caccagagcg cgcgaagcgc ctcgaggatc
     1861 ggccgcggca gggagacgcg gcgatccctg ccgcccttgc cggcgacgat gcgcagcacc
     1921 tcctgctcgc ggtcgacggc atcgagctgg agctttacgg cctcgccgat gcgcaggccg
     1981 caaccataca tgacctgaag gcaggtgcgg tgcttcggac tccttacgca agccagcagc
     2041 cggcgaacct ggccgtcgct gagggactcg ggaagacgct tctgcttcgg caggcggatg
     2101 cggttttttt tgagaacaac ggccagtcac ggccgagcgt ggtcgtgaag aagaacttga
     2161 tgccgaaatg gacgcgcttg aacgtgccac gcgcgacgcc gtcgtcgatc acgccgacca
     2221 ggaacgcgcg cacctcctgc tcgtccaggc gatccggacg ccggtgatag tgcatcgcca
     2281 gccgcttcac cgcctgcgtg taagcctgct gcgtcctggg tgaaagtccg gccagcttca
     2341 tgtcgccgat catccgctcg cgcaacggtg tcatgacgct cctccccccg gtggccaccg
     2401 ccgggcgctt gccgcccggc ccgccgaagg gatactcgcc catcaccaga gccgcgagcc
     2461 ggagcgcgct gccgactcgg cgaagccgtt cagtccaacg tcgcggcgcc gggcgagcgg
     2521 tagaacgtgc cattgacctc gatcgaggtc agcctgcggc tggcgaactc gagctcgcgc
     2581 ttgtgcgcga ggccggcggg gtagaaggcg ccgcgccaag gctcgtacgt ccagccgccg
     2641 atcccgaccc ggatcgtgcc cgcttccctt ccggccatgg ctgccctccg ctcgctgccg
     2701 cggatgtggt catggcccgg gcggcgtgat aggtccaggt tcaccttccg ttc
//
LOCUS       DATIAZ010000147         5062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_564057, whole genome shotgun sequence.
ACCESSION   DATIAZ010000147 DATIAZ010000000
VERSION     DATIAZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564057"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VLF19_05140"
     CDS             <1..359
                     /locus_tag="VLF19_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92670.1"
                     /translation="GYRPLPEWEALFDWLVAARTRPTLARLAMFQFAGETQSCEGFET
                     LITLARDVDPETADLYARAILPDEYRHAAIGRRALHLLADTAERQATARAACREMNER
                     VFAAYRAHRARVDARG"
     gene            777..1328
                     /locus_tag="VLF19_05145"
     CDS             777..1328
                     /locus_tag="VLF19_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92671.1"
                     /translation="MSTPSIETIPVKALFDSALKASFEDLQISDRGTMGHLADLLARF
                     AAAGDLWPVGVAGTRLQSLADRVLEIQRSWQLDGRHFDPRREVELRQGIGDYTLFMTG
                     FFWERVKAESLTRHYVREGKRAYRFVAEFYRGLGRPEALRFATVAARFETCAAVLSYM
                     RDIYLDAEFAPWPHRAFARIISR"
     gene            complement(1600..2214)
                     /locus_tag="VLF19_05150"
     CDS             complement(1600..2214)
                     /locus_tag="VLF19_05150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSE92672.1"
                     /translation="MGARRTLIYLLRHGEVVLAEAGRFIGHLDVTLSERGERQSAAQA
                     ERLRTTPLTAVYTSDLARARRTGEIIGGRHGLVPTAVPALREMAMGRWDGLTAQEIMA
                     REPAAFSDWMARVGEFPFPEGESVGDLLARAWPAFEALADGHDGASIAIVAHGGTNRA
                     VLCRALGLPLARLLAFGQDYGALSVLARDDHGWRLLRLNEPPGL"
     gene            complement(2193..2933)
                     /gene="cobS"
                     /locus_tag="VLF19_05155"
     CDS             complement(2193..2933)
                     /gene="cobS"
                     /locus_tag="VLF19_05155"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HSE92673.1"
                     /translation="MSGLVLAAQYLTIVPLGRNPTHEDAAGRSAAWFPVVGLGIGLAI
                     LVVERVTGRIFPVLLGALFTVTAWKLLTGGLHLDGLADCLDGLAGRDADQRIRIMRDS
                     RIGAFGAMGLILFLLTEVVAVAELAPALRWRAVLAAPVIARAMPPLLARWFPPARPDG
                     HGAAFAAGVQPVAVPLALGVAAVVGLGALGLLGIVALAVAALAALAVGWFMAGRLDGI
                     TGDVHGAAVEVAELTVLLTVAAWVHGAR"
     gene            3068..4813
                     /locus_tag="VLF19_05160"
     CDS             3068..4813
                     /locus_tag="VLF19_05160"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HSE92674.1"
                     /translation="MSVTSVAEKWVNEVAALTQPSRIVWCDGSKAEYDGLIESMLRDG
                     TLVPLDQSTYPSCYLHRSHPSDVARTEQLTYICTREKDDAGPTNNWMSPAEGRARTTP
                     WLRGSMRGRTMWVIPYLMGPAGSVMSRVGIMVTDSAYVVASMHLMTRVGPVATQHMRA
                     DDDFIAGLHSLGDLSPDRRLILHFPEEKLIWSVGSGYGGNALLGKKCHALRIGSWQAR
                     QEGWLAEHMLILGVEDPEGRVTYLAAAMPSASGKTNLSMVVSSLPGWRAWTVGDDIAW
                     MHVDASGQLRAINPERGFFGVAPNTSPKTNPNGTAMVRSNTIFTNVAMTPDGQPWWEG
                     LTPQPPAGLIDWQGRPWDPAQGPAAHPNSRFTVLAQQCPSISPNWEDPQGVPISGLIF
                     GSRRTGVIPLVFEGFDWTHGVFLGSAMSTETTAAITGEVGVVRRDPMAMLPFCGYNMA
                     DYFGHWLATGRKLSRPPRIFRVNWFRRDADGKFLWPGYRDNMRVLKWMVERIRGRADG
                     AEETPIGWVPAPSALDTSGLDLPAARLREALRVDGGEWLAALDDLGQFYDQFGPRLPE
                     PIAKTLAETRRRFGA"
     gene            complement(4817..>5062)
                     /locus_tag="VLF19_05165"
     CDS             complement(4817..>5062)
                     /locus_tag="VLF19_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSE92675.1"
                     /translation="QGTVWGWLIGPFVDAWLRVRPHDRAGARRALEGFVPHLGEACLG
                     SISEIFDADPPFTPRGCVAQAWSVAEVLRCWVKTAER"
BASE COUNT          772 a         1873 c         1745 g          672 t
ORIGIN      
        1 acggctatcg tcccctgccc gagtgggagg cgctcttcga ctggctcgtg gccgcccgca
       61 cccgcccgac gctcgcgcgc ctggcgatgt tccagttcgc cggcgagacc cagtcgtgcg
      121 agggcttcga gacgctcatc acgctggcgc gcgacgtcga cccggagacg gccgatctct
      181 atgcgcgcgc catcctgccc gacgagtacc gacacgccgc gatcgggcgc cgcgcgctcc
      241 acctgctggc cgacacggcc gagcgccagg ccaccgctcg cgccgcctgc cgggagatga
      301 acgagcgcgt cttcgccgca taccgggcgc accgcgcccg ggtggacgcg cgaggctgag
      361 ctcctaccac ggggtcgccc ccgttcatct gtgcacgagt gtgccctcgg ggcatgcaag
      421 cgccgatcga ccctccgctc gcgtctacct cgtgccgctc gggcttgtcc ctccggcgat
      481 cgtcggggac ctcgtcgcag gcggggggac gccggccaat cgcgcttgac agcgcagctt
      541 cagagggaag gatcgacgcc gaaaccgctg tttgcaatcg atcgcctgag gactcactga
      601 agcctcgtcg ccgatcgggc cgtgcgctgg tggtcggcgc cgaagccgcg gacctcgggt
      661 cgagcggacc gtacccccgc tggagacccg tccgcattcg taccccggct ggctgtcgag
      721 ttcaggaccc tcgccaggcg tccgataacc actgcgagga gggcatccgg caagcgatga
      781 gcacgccgag catcgagacg atcccggtga aggccctgtt cgacagcgcc ctgaaggcca
      841 gcttcgagga cctccagatc tccgaccgcg ggacgatggg ccacctggcg gacctgctgg
      901 cccgcttcgc ggcagccggc gacctctggc cagtcggcgt cgccggcacc aggctgcaga
      961 gcctggcgga ccgcgtgctc gagatccagc gctcgtggca actcgacgga cggcatttcg
     1021 atccccggcg cgaggtcgag ctgcgccagg gcatcggcga ctacacgctc ttcatgaccg
     1081 ggttcttctg ggagcgggtg aaagcggagt cgctgacccg tcactacgtg cgcgagggca
     1141 agcgcgcgta ccgcttcgtc gccgagttct accgcggcct gggccggccc gaggccctgc
     1201 gcttcgcgac ggtggccgcg cgcttcgaga cgtgcgccgc cgtgctctcc tacatgcgcg
     1261 acatatatct cgacgcggag ttcgcgccgt ggccgcatcg cgccttcgcg cgcatcatct
     1321 cccgataggc tccggcgccc gggacccgag tgcgcggcca agccgacgct cgagcgccgc
     1381 aagctcgctg gcggctccgc ctccagaacg gattgcgctg gcaaagccgg cgatcgagcg
     1441 atagtcgccc gccgggctca cagagcgctt cggaaggggc cgtagccccc gcgcgagatg
     1501 gctagtcacc gaaaggcgtc tcgccattcc gaccgaactg ctctctcctg agttgccggt
     1561 ccgccgaccg taggcctagc gagctcctga ctcacgacac tacaggccgg gcggctcgtt
     1621 gaggcggagc aggcgccagc cgtggtcgtc gcgggccagc acggagagcg cgccgtagtc
     1681 ctggccgaag gccaacaggc gtgcgagcgg caagcccagc gcgcggcaca gcaccgcccg
     1741 gttcgtcccg ccgtgcgcga cgatggcgat gctcgcgccg tcgtggccgt cggcgagcgc
     1801 ctcgaaggcg ggccaggcgc gcgcgagcag gtccccgacg ctctcgccct ccggaaacgg
     1861 gaactcaccc acccgcgcca tccagtcgct gaaggctgcg ggctcgcgcg ccatgatctc
     1921 ctgcgccgtc agcccgtccc agcgccccat cgccatctcg cgcagcgcgg gaacggccgt
     1981 cggcacgagc ccgtggcgcc ccccgatgat ctcgcccgtg cgtcgggccc gcgccagatc
     2041 gctcgtgtac acggcggtca gcggcgtggt ccgcaggcgc tcggcctggg ccgcggactg
     2101 acgctcgccg cgctcggaca gcgtcacgtc gaggtgaccg atgaagcggc ccgcctcggc
     2161 gagcaccacc tcgccgtgac gcaagaggta gatcagcgtg cgccgtgcac ccacgccgcc
     2221 accgtcagga gcacggtcag ctcggcgacc tccacggcgg cgccgtgcac gtcaccggtg
     2281 atgccgtcga gccggccggc catgaaccac ccgacggcca gcgcggccag ggcggccaca
     2341 gcgagggcga cgatgccgag gagtcccagc gcgccgagcc cgacgaccgc cgcgacgccg
     2401 agcgccagcg gcacggcaac gggttgcaca ccggccgcga acgccgcgcc gtgaccgtcc
     2461 gggcgcgccg gcggaaacca gcgcgccagc aggggtggca tcgccctcgc gatgacgggc
     2521 gccgccagga cagcgcgcca gcgtagcgcc ggcgccagct cggcaacggc gacgacctcc
     2581 gtcaggagga agaggatgag gcccatggcg ccgaacgccc cgatccggct gtcacgcatg
     2641 atgcgaatgc gctggtcggc atctcgcccg gcgagcccgt cgagacagtc ggccaggccg
     2701 tcgaggtgca gtccgcccgt cagcagcttc caggccgtca ccgtgaagag ggcgccgagc
     2761 agaaccggaa agatccgtcc cgtgacccgc tcgacgacca ggatcgccag cccgattccc
     2821 agtccgacga ctggaaacca agcggccgac cgcccggcgg cgtcttcgtg ggtcgggttg
     2881 cgtccgagcg gcacgatcgt gaggtactgc gcagccagca cgaggccgga cacgacgtga
     2941 tgctagcacg ttccccttga cgatcgcggc ggtccggcgc acccttggag cctaatcccc
     3001 cggatacggc gccgccctcg gggcggccgg tcggaagctc acatagagac agggcgagga
     3061 tactccgatg agcgtgacca gcgtggcgga gaagtgggtg aacgaggtcg ccgcactcac
     3121 ccagccgtcc cggatcgtgt ggtgcgacgg ctcgaaagcc gagtacgacg gcctgatcga
     3181 gtccatgttg cgggatggca cgctcgtccc gctcgaccag agtacctacc cgagctgtta
     3241 cctgcaccgc agccaccctt ccgacgtcgc ccgcaccgag cagctcacct acatctgcac
     3301 gcgggagaag gacgacgcgg gaccgacgaa caactggatg tcgccggccg agggcagggc
     3361 ccggacgacc ccgtggctgc gcggcagcat gcgcggccgc accatgtggg tgattccgta
     3421 cctgatgggc ccggccggct ccgtgatgag ccgcgtcggc atcatggtga ccgacagcgc
     3481 gtacgtcgtg gccagcatgc acctgatgac gcgcgtgggg cccgtggcca cccagcacat
     3541 gcgcgccgac gacgacttca tcgcgggcct gcactcgctc ggcgacctct cgcccgaccg
     3601 gcgcctcatc ctgcacttcc ccgaggagaa gctcatctgg agtgtcggct ccggctacgg
     3661 gggcaacgcg ctgctgggca agaagtgcca cgcgctgcgc atcggctcgt ggcaggcgcg
     3721 ccaggaaggc tggctggccg agcacatgtt gatcctcggc gtcgaggacc ccgagggtcg
     3781 cgtcacctac ctcgccgccg ccatgccgag cgcatccgga aagaccaatc tgtcgatggt
     3841 cgtgtcctcg ctgccgggct ggcgcgcgtg gaccgtgggc gacgacatcg cgtggatgca
     3901 cgtcgacgct tccggccagc tccgcgccat caacccggag cgcggcttct tcggcgtggc
     3961 gccgaacacg agcccgaaga ccaaccccaa cggcacggcg atggtgcgct cgaacaccat
     4021 cttcaccaac gtcgcgatga cgccggacgg acagccgtgg tgggagggtc tcacgcccca
     4081 gccaccggcg ggactcatcg actggcaggg ccggccgtgg gacccggcgc agggaccggc
     4141 cgcgcacccg aactcgcgct tcaccgtgct cgcccagcag tgcccgtcga tctcgccgaa
     4201 ctgggaggac ccgcagggcg tgccgatctc cggcctcatc ttcggctccc ggcgcaccgg
     4261 ggtcatcccg ctggtcttcg aggggttcga ctggacgcac ggcgtgttcc tcggctccgc
     4321 catgagcacc gagaccacgg cggccatcac cggcgaggtg ggcgtggtgc ggcgcgatcc
     4381 gatggccatg ctgccattct gcggctacaa catggccgac tacttcggcc actggctggc
     4441 caccgggcgc aagctcagcc ggccgccccg gatcttccgc gtgaactggt tccgccgcga
     4501 cgccgacggc aagttcctct ggccgggcta ccgcgacaat atgcgcgtgc tcaagtggat
     4561 ggtcgagcgc atccgcggcc gggccgacgg cgccgaggag acgcccatcg ggtgggtccc
     4621 ggcgccgagc gcgctcgaca cgtcggggct cgacttgccg gcggcgcggc tgcgcgaggc
     4681 gctccgtgtg gacggcggcg agtggctcgc cgcgctggac gatctcggcc agttctacga
     4741 ccagttcggg ccgcgcctgc ccgagccgat cgcgaagacg ctggccgaga cccggcgccg
     4801 cttcggagcc tgaggcctac cgctcggccg tcttcaccca gcagcgcagc acctcggcca
     4861 cgctccacgc ctgggcgaca cagccgcgcg gcgtgaaggg cgggtccgcg tcgaagatct
     4921 cgctgatcga tccgaggcag gcctcgccga ggtgcggcac gaagccctcg agcgcgcggc
     4981 gcgcgccggc ccggtcatgc gggcgcaccc gcagccacgc atccacgaac ggtccgatga
     5041 gccaccccca caccgtgccc tg
//
LOCUS       DATIAZ010000148         5378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_169251, whole genome shotgun sequence.
ACCESSION   DATIAZ010000148 DATIAZ010000000
VERSION     DATIAZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169251"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VLF19_05170"
     CDS             complement(<1..346)
                     /locus_tag="VLF19_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE92676.1"
                     /translation="MLLRVAGLTKRFGGVVANHAVTFDVAPGELVGIIGPNGAGKSTL
                     FDLVTGFARPDAGRVAVDGRDVTRLRPDEICRLGVARTFQKLRPFAGMTALENVMVGA
                     FQRTGDVGEARDI"
     gene            complement(346..1287)
                     /locus_tag="VLF19_05175"
     CDS             complement(346..1287)
                     /locus_tag="VLF19_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92677.1"
                     /translation="MRRGLLLALFVAALAFPFVFAKPFPRHVMIMIFLYAALAGAWNI
                     LAGYCGQISLGHAVYFGVGAYTSTVLVREANLTPWLGMLAGAALAVLVSQTIGYPVFR
                     LRGHYFAIATIAVGEIVQTLVINWDRVGGARGLFVPIQRPDSLLNFQFHESRAAYYFV
                     ALGLLLLTLAVTRALERSRRGYLFRAVREDQEAAASLGVHVAREKHRALAVSAALTAL
                     GGTFYAQYVLFIDPESVFPLSLSILICLVAVLGGVGTLWGPLLGAAVLVPISEGTRVW
                     LGGGGKALDLLIYGALIMVIAVVQPAGLVGLLRARAR"
     gene            complement(1299..2120)
                     /locus_tag="VLF19_05180"
     CDS             complement(1299..2120)
                     /locus_tag="VLF19_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92678.1"
                     /translation="MGLVYALIAAGLSLIFGLMEVVNFAHGEFLMLAMYVAFFAWALA
                     RLDPVISFPLVALLLAALGWATYHGLIRRVLGGPMLAQIFATFGLAVFLRSVAQFLWG
                     VDFRVIATPWLGGRIEAGGIFVGLPQAAAGCAALLAFGGLTLFLARSETGLALQATAQ
                     DRQAAALMGIDTERMFALGWAIGAGCTGVAGGLLAMFFYVFPDVGGSFALLAYVTVAL
                     GGFGNVPATLLAGVVVGLVEAFAGLLIEPALKYAVVFLLYLAVVLWRPQGLFGRF"
     gene            complement(2162..3427)
                     /locus_tag="VLF19_05185"
     CDS             complement(2162..3427)
                     /locus_tag="VLF19_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92679.1"
                     /translation="MSRSLAVSLSLLVLLAVAAGPAAAQPKEVVIGVLYPMSGPAAQA
                     GVDDKPVFEIAAEIANGSVDLSFPLYQKLKGMPGLKGAKVRLIFADHQGKPDVGQAEA
                     ERMITQEKVAALVGCWHSAVTATASQVAERHGVPFMNPESSSPGLTARGFKWFFRTSP
                     HDGHFTQVMFDFFRDFAKKRGVTLRTLGLTYEDTLFGADSGKVQKELAKKHGYEVVLD
                     LQYRARATSLASEVQRLKAANPDVWMPTSYQTDAILFTRTMKELDYNPRMVMAQNAGH
                     ISPDFVAAVGKDAEGTLTRAPFSTDLVEKRPVARAVNAIYAKRAGKDLYDFPARAFTG
                     MITLLDAINRAGSTDPEAVRKALVATQIAGDQLVMTWDTVKFDESGQNTGVKGIILQL
                     QGGKYHTVYPFDVATKDVIYPIPAWKDRK"
     gene            3529..3861
                     /locus_tag="VLF19_05190"
     CDS             3529..3861
                     /locus_tag="VLF19_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92680.1"
                     /translation="MILLAMALTLMLVGTGLSSPDIAAAQAVQHGAQPNPEEVRPANP
                     DGDSPSAAAGSLRDTRERRILILGLPVNAVLLIAGVLVVLLLAAGAVLPRARRRERAR
                     GGGTFGRP"
     gene            complement(3973..4677)
                     /locus_tag="VLF19_05195"
                     /pseudo
     CDS             complement(3973..4677)
                     /locus_tag="VLF19_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
     gene            complement(4727..>5378)
                     /locus_tag="VLF19_05200"
     CDS             complement(4727..>5378)
                     /locus_tag="VLF19_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSE92681.1"
                     /translation="LAEEYIPGVEVALEGMLTDGGLQTLALFDKPDPLEGPFFEETLY
                     VTPSRLPAAIQGAIADAARSAAAALGLEHGPIHAELRVNERGPCVIELAARSIGGLCS
                     RTLRFGTGMTLEELILRHALGWRIATFDRERRAAGVMMVPIPGPGRLLAVRGTDAAEA
                     VPDIEAVAITVHVGQELIPLPEGWQYLGFIFARAGTPDAVERALRTAHALLRFDIA"
BASE COUNT          849 a         1861 c         1970 g          698 t
ORIGIN      
        1 cgatgtcacg agcttcgccg acgtcaccgg tgcgctggaa ggcaccgacc atgacgttct
       61 cgagcgccgt catgcccgcg aagggccgga gcttctggaa cgtgcgtgcg acgccgagcc
      121 ggcagatctc gtccggtcgc agccgcgtga cgtcgcggcc gtccacggcc acgcggcccg
      181 cgtcggggcg ggcgaagccc gtgacgaggt cgaagagggt cgatttgccg gcgccgttgg
      241 ggccgatgat cccgaccagc tcgcccggcg ccacgtcgaa cgtgacggcg tggttggcga
      301 ccacgccgcc gaaccgcttg gtgagcccgg cgacgcggag cagcatcagc gtgccctcgc
      361 gcgcaggagc cccaccagcc ccgcaggctg gacgacggcg atgaccatga tcagcgcgcc
      421 gtagatcagc aggtcgagcg ccttgccgcc gccgccgagc cagacccgcg tgccctcgct
      481 gatcggcacc aggaccgccg cgccgagcag gggcccccac agcgtcccca cgccgccgag
      541 cacggcgacc aggcagatga ggatggagag cgagagcggg aacacggact cgggatcgat
      601 gaagaggacg tactgggcgt agaaggtgcc gccgagcgcc gtgagcgccg ccgagacggc
      661 gagcgcccgg tgcttctcgc gcgcgacgtg gacgcccagg ctcgccgccg cctcctggtc
      721 ctcgcggacc gcgcggaaga ggtagccgcg gcgcgaccgc tcgagcgcgc gcgtcacggc
      781 gagcgtcagc agcagcaggc ccagcgcgac gaagtagtag gcggccctgg actcgtggaa
      841 ctggaagttc aggaggctgt cggggcgctg gatgggcacg aacaggccgc gcgccccgcc
      901 cacccggtcc cagttgatga ccagcgtctg cacgatctcg ccgacggcga tggtggcgat
      961 ggcgaagtag tggccacgca ggcggaagac gggatagccg atggtctgcg agaccagcac
     1021 ggccagcgcg gcgcccgcca gcatgccgag ccacggcgtg agattcgcct cgcggaccag
     1081 cacggtggac gtgtaggcgc cgacgccgaa gtagaccgcg tgcccgagcg agatctggcc
     1141 gcagtagccg gccaggatgt tccacgcgcc ggccagcgcg gcgtagagga agatcatgat
     1201 catgacgtga cgggggaatg gcttggcgaa gacgaacggg aaggcgaggg cggcgacgaa
     1261 cagggcgagc agcaggccgc gccgcaccgc cgccggcctc agaagcgccc gaagagccct
     1321 tgcggccgcc agagcacgac cgcgaggtag agcaggaaca cgaccgcgta cttcagcgcc
     1381 ggctcgatga ggaggcccgc gaaggcctcc acgagaccga cgacgacgcc ggccaggagc
     1441 gttgccggca cgttgccaaa gccgccgagg gcgaccgtga cgtaggcgag cagggcgaag
     1501 ctcccgccca cgtcggggaa gacgtagaag aacatcgcca gcagcccgcc ggcgacgccc
     1561 gtgcaccccg cgccgatcgc ccagccgagt gcgaacatcc gctcggtgtc gatgcccatc
     1621 agcgccgccg cctggcggtc ctgcgccgtt gcctggaggg ccagaccggt ctcagagcgc
     1681 gcgaggaaga gcgtgagccc gccgaacgcc agcagggccg cgcagccggc ggccgcctgg
     1741 ggcaggccca cgaagatccc gcccgcctcg atgcggccac cgagccaggg ggtggcgatg
     1801 acgcggaagt ccacgcccca gaggaactgg gcgaccgagc gcaggaagac ggcgagcccg
     1861 aacgtcgcga agatctgcgc gagcatcgga ccgccgagca cccggcggat caggccatgg
     1921 taggtcgccc agccgagcgc ggcgagcagc agcgccacga gcgggaacga gatcaccggg
     1981 tcgagccgcg cgagtgccca ggcgaagaag gccacgtaca tggcgagcat caggaactcg
     2041 ccgtgggcga agttgacgac ctccatgagg ccgaagatca gcgagaggcc ggcggcgatc
     2101 agcgcgtaca cgaggcccat cagcacgccg ccgaccgccg cctgcaggaa gacgtcgggc
     2161 gctatttacg gtccttccac gcggggatcg ggtagatcac gtccttcgtc gccacgtcga
     2221 agggatagac ggtgtggtac ttgccgccct gcagttgcag gatgatgccc ttcacgcccg
     2281 tgttctgccc gctctcgtcg aacttcacgg tgtcccaagt catgacgagc tggtcgccgg
     2341 cgatctgcgt cgccaccagc gccttgcgga ccgcctcggg gtcggtcgag cccgcgcggt
     2401 tgatggcgtc gagcaacgtg atcatgccgg tgaaggcccg ggcgggaaag tcgtagaggt
     2461 ccttgccggc gcgcttggca tagatggcat tgaccgccct ggccacgggc cgcttctcga
     2521 cgagatctgt cgagaacggc gctcgcgtga gcgttccctc cgcgtccttg ccgaccgctg
     2581 cgacgaaatc cggtgagatg tggcccgcgt tctgggccat gaccatcctg gggttgtagt
     2641 cgagctcctt catcgtccgc gtgaagagga tcgcgtcggt ctgataggag gtcggcatcc
     2701 agacgtcggg gttggcggcc ttgaggcgct gcacctcgga cgcgagcgac gtggcccgcg
     2761 cgcggtactg gaggtcgagc acgacctcgt agccgtgctt cttcgccagc tccttctgga
     2821 ccttcccgct gtcggcgccg aacagcgtgt cctcgtaggt cagccccagc gtcctgagcg
     2881 tcactccccg cttcttcgcg aagtcgcgga agaaatcgaa catcacctgc gtgaagtgcc
     2941 cgtcgtgcgg ggaggtgcgg aagaaccact tgaacccgcg cgcggtgagc cccggcgacg
     3001 acgactcggg gttcatgaac ggcacgccgt gacgctcggc cacctggctc gccgtcgcgg
     3061 tgaccgccga gtgccagcag ccgacgagcg cggcgacctt ctcctgcgtg atcatgcgct
     3121 cggcctcggc ctggccgacg tcgggcttgc cctggtgatc ggcgaagatc agccggacct
     3181 tcgccccctt gagccccggc atgcccttga gcttctggta gagcggaaac gacaggtcca
     3241 cgctgccgtt ggcaatctcg gcggcgatct cgaagacggg cttgtcgtcg acgcccgcct
     3301 gggcggccgg accggacatc gggtagagca cgccgatgac gacctccttg ggctgcgccg
     3361 cggcggggcc cgcggcgacc gcaagcagca ccagcaggga gagactgacg gcgagcgacc
     3421 ggctcatggg cggcacctcc gtgcgcggaa tgctagcact gtcgatgtct cttggcaccg
     3481 gcattgctcc gccttcctcg agatgcccgg cttgactgga gggggtgcat gatcctgctc
     3541 gcgatggccc tcacgctgat gcttgtcggg accggtctgt cgtcgcccga catcgctgcc
     3601 gcgcaggccg tgcagcacgg cgcccagccc aacccggagg aggtgcggcc agcgaacccc
     3661 gacggcgaca gcccgtcggc agccgcgggc agcctgcgtg acacccgaga gcggcggatc
     3721 ctgatcctgg gcctgccggt gaacgcggtg ctcctgatcg ccggagtgct cgtggtcctg
     3781 ctgctcgccg ccggtgccgt gcttccccgc gcccgccggc gtgagcgcgc gcgcggaggt
     3841 ggcacgttcg gccggcccta gcaccgacgt cgtcgccgcc actgtgggcg aggcccgctt
     3901 ggctcggggc gacctggagg aggcgcgcgc gggccggcca ccgccagtca gtctggtgtc
     3961 ggcggcccgg gctcaggaac cgaggcgcac gagcgactcc ctgacggtgg gccggccccc
     4021 ggcctccacg tcgctgcgcc gcaggcgatg aaaggcgccg aggaccgagt tggcgcgtgc
     4081 cagctccacg acccgcgctg tgtattccgc gagccgcccg ccgggaaccg cgaagatcgc
     4141 cacgtcgtcg gtcagggcgt ccagataggc gcgcgcgccg cagcagccga ggctgagcgc
     4201 ggcgcgcccg ctgttgacca cctgcgggac ggctgcgcag gccggccgcc ccatcgccgg
     4261 cgggacgccg tgatcgacgc cctcggccgc ctcgacgagg acccgcgtcg ggctcgcctt
     4321 gacgaacatc acgacggcgt cgggcgacat gggcgtgtcg gcgagcggcg cgtagacgac
     4381 gtgacgccgg cgctccgaga gcgtcgggat cagcgggatg tcctccgggc gtacgtagcc
     4441 gaggtcgacg aagacctgca gcgcggtccg ccggtcctcc tcgtgggcgg ggctcaccgc
     4501 caggttgtgg gtgaaggtgc cgatgccgca gaggtcgtga tcgaccggcg acgtcgcgaa
     4561 cggcccgcgc gccgcctcct gccagaacac ggagccggcg ggcactcgcc ccgtgaacgc
     4621 cggcacgccg gccggcacgc tgtcggtcag gcagacggcg accggcggcc gcgcgagatc
     4681 gagggcctcg ggagcgtgcg cccgaggatg gcgtaatccg ggattttcag gcgatgtcga
     4741 agcgtaggag ggcgtgcgcg gtccgcagcg ccctctcgac ggcgtccggc gtgccggcgc
     4801 gcgcgaagat gaagccgagg tattgccagc cttcgggcaa ggggatcagc tcctggccca
     4861 cgtgtacggt gatcgcgacc gcctcgatgt ccggcaccgc ctcggcggcg tccgtgcccc
     4921 ggacggcgag cagccgtccg gggccgggga tcgggaccat catcaccccg gcggcgcggc
     4981 gctcgcggtc gaacgtcgcg attctccagc cgagcgcatg tctcaggatc agctcttcga
     5041 gggtcatgcc agtgccgaag cgcagcgtcc gcgagcacag tccgccgatg gagcgggcgg
     5101 ccaactcgat cacgcacggc ccgcgctcgt tgacgcgcag ctcggcgtgg atcggcccgt
     5161 gctcgaggcc gagcgccgcg gcggccgacc gcgccgcgtc ggcgatcgcg ccctggatgg
     5221 ccgcaggcag acgggatggc gtgacgtaga gcgtctcctc gaagaacggg ccctcgagcg
     5281 ggtccggctt gtcgaagagc gcgagggtct gcagtccgcc gtccgtgagc atgccttcga
     5341 gcgcgacctc gacgccgggg atgtactcct cggcgagc
//
LOCUS       DATIAZ010000149         4845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_827477, whole genome shotgun sequence.
ACCESSION   DATIAZ010000149 DATIAZ010000000
VERSION     DATIAZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827477"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /gene="thrS"
                     /locus_tag="VLF19_05205"
     CDS             <1..1358
                     /gene="thrS"
                     /locus_tag="VLF19_05205"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSE92682.1"
                     /translation="RHFKLLSSSGAYWRGDEKNPMLQRIYGTAWLTASELEQHLWRLD
                     EAKKRDHRKLGRELDLFDFYDIAPGAPFWLPSGMVLVRELERFAREHLDARGYQEIST
                     PMLVNKKLWEQSGHWEHYQDSMFKIEVEGEIFSLKPMNCPESTYVYRRTLRSYRDLPL
                     RLSEMGRDHRNERSGTLTGLVRVRQFTQDDAHIYCRPDQLQDEITALLDLVREWYKTF
                     DLQPSYKLSTRPEKRLGIEAQWDSAESALHEALRGNGLAYVVDPGGGNFYGPKIDIDV
                     QDALGRGWQVATIQVDLTMLPERFQLEYIDADGQPKRPVAIHRAIFGSYERFIGILIE
                     HYAGAFPTWLAPVQARVLPISEKHTGYARSVWERLRAARVRAELDDRNEKLGYRIREA
                     QLRKVPYMLVVGGREAEAGTVSLRHRTGEELKDVAVDRVLADLAREIGARASSLTVGR
                     A"
     gene            1484..2005
                     /gene="infC"
                     /locus_tag="VLF19_05210"
     CDS             1484..2005
                     /gene="infC"
                     /locus_tag="VLF19_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSE92683.1"
                     /translation="MSQAKEIRVNEGIRVREVRVVSADGEQLGIMPTQQALELARTRE
                     FDLVEVAPEAQPPVCRIMDFGKYKYTQARRLKEARKKQTTIQIKEVKMGPKTEKHDFD
                     FKLKHVRRFLEDGHKAKVTVRFKGREMAHTELGWKMLQKMVEAVQDIALVENNPRMEG
                     RMLHIMLSPKAHN"
     gene            2022..2219
                     /gene="rpmI"
                     /locus_tag="VLF19_05215"
     CDS             2022..2219
                     /gene="rpmI"
                     /locus_tag="VLF19_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSE92684.1"
                     /translation="MPKMKTKRGAAKRLKKTASGKLRRAGAWKQHKLEGKDPHRRRRL
                     RKAKLITKADEPRLTVLVPYL"
     gene            2248..2601
                     /gene="rplT"
                     /locus_tag="VLF19_05220"
     CDS             2248..2601
                     /gene="rplT"
                     /locus_tag="VLF19_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSE92685.1"
                     /translation="MPRAKGGSKTRQRRKKVLKKAKGYVGGRHRLYRPAKETVLRAGA
                     FAYRARRQKKRLARALWIVRINAACRQTGLSYSTFMAALRKAGILLDRKVLAELAVAD
                     PAAFAKLTETAKANA"
     gene            2608..3633
                     /gene="pheS"
                     /locus_tag="VLF19_05225"
     CDS             2608..3633
                     /gene="pheS"
                     /locus_tag="VLF19_05225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSE92686.1"
                     /translation="MAHPRVDAIAEEARAAIARTGSSAELEQLRVRYLGRQGALTLLL
                     RSLGSLPAAERPLVGAAANEAKRALEALLDMRLGEMREAERRHERARRRLDLTLPGRR
                     PQLGTLHPLTRVHDEIVAIFVGLGFSVAEGPEVEDDYHNFEALNIPRDHPARDMQDTF
                     YLSDDVLLRTHTSPVQIRTMQAQRPPVRIVVPGRVYRRDELDPSHSPVFHQVEGLAVD
                     RHITMGDLKGTLDLFARQMFGPRSRVRFRPSFFPFTEPSAEVDVLCFLCGGDGCRVCK
                     QSGWLEILGSGMVHPRVLQNVGYDPEEVTGWAFGMGIERIAMLKYGVDDIRLFFENDL
                     RFLRQFA"
     gene            3630..>4845
                     /gene="pheT"
                     /locus_tag="VLF19_05230"
     CDS             3630..>4845
                     /gene="pheT"
                     /locus_tag="VLF19_05230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSE92687.1"
                     /translation="MKIPYRWIKEFVEVDLTPQQAAERLTNAGIEVAAVIPLAPAGLA
                     GVVVGEIEAIERELGDSRGHRVVLCRVTTGRERFGVVCGAPNVALGLRAAFAPPGARL
                     PEPAGGSRAVAAARIRGAESQGMLCSERELGVGEEHEAGLLAFPADAPLGADVVSALG
                     LDDHVLEVEVTPNRPDCLSVVGVARELAALTGAPFRSPATAVKEVDEPVTGLAQVRIE
                     APDLCHRFTARVISGVRVGPSPAWLAARLRAVGLRPISNVVDATNYVMWEIGQPLHAF
                     DHARLAEATIVVRRARAGERFTTLDGQDRTLSGEMLVIADPAKAVGIAGVMGGADTEV
                     TDRTTRVLLEAAWFLPASVRRTSRALGLATDAAYRFERGADIEALVDASDRAAQLIAD
                     LGGGRVARGMVDV"
BASE COUNT          751 a         1706 c         1752 g          636 t
ORIGIN      
        1 tccgccactt caagctgctc tcgtcctccg gcgcctactg gcgcggcgac gagaagaatc
       61 cgatgctgca gcggatctac ggcacggcct ggctcacggc gtcggagctc gagcagcacc
      121 tgtggcggct ggacgaggcg aagaagcgcg accaccgcaa gctcgggcgc gagctggacc
      181 tgttcgactt ctacgacatc gcgcccgggg cgccgttctg gctgcccagc gggatggtcc
      241 tggtacgcga gctggagcgg ttcgcccggg agcacctgga cgcccgcggc taccaggaga
      301 tctccacccc gatgctcgtg aacaagaagc tgtgggagca gtcggggcac tgggagcact
      361 accaggacag catgttcaag atcgaggtgg aaggcgagat cttcagcctg aagcccatga
      421 actgtcccga gtccacctac gtctaccggc gcacgctgcg ctcgtaccgc gacctgccgc
      481 tgcgcctgag cgagatgggg cgcgaccacc gcaacgagcg ctcgggcacg ctcacggggc
      541 tcgtgcgcgt gcggcagttc acgcaggacg acgcccacat ctactgccgg cccgaccagc
      601 tgcaggacga gatcacggcg ctgctcgacc tcgtccgcga gtggtacaag acgttcgacc
      661 tgcagccgtc ctacaagctc tcgacgcgcc ccgagaagcg cctggggatc gaggcccagt
      721 gggattccgc cgagagcgcg ctgcacgagg cgttgcgtgg caacggcctc gcgtacgtgg
      781 tcgaccccgg cggcggcaac ttctacgggc cgaagatcga catcgacgtg caggacgccc
      841 tcggccgcgg ctggcaggtg gccaccatcc aggtggacct caccatgctg cccgagcgct
      901 tccagctcga gtacatcgac gccgacggcc agcccaagcg cccggtggcc atccatcggg
      961 cgatcttcgg gtcgtacgag cgcttcatcg gcatcctcat cgagcactac gcgggcgcgt
     1021 tccccacgtg gctggcgccc gtgcaggcgc gcgtgctgcc gatcagcgag aagcacaccg
     1081 gctacgcgcg gagcgtgtgg gagcggctgc gggcggcccg cgtccgcgcc gagctggacg
     1141 accgcaacga gaagctcggc taccgcatcc gcgaggcgca gctccggaag gtgccctaca
     1201 tgctcgtcgt cggcgggcgc gaggccgagg cggggaccgt cagcctgcgc caccgcaccg
     1261 gcgaggagct gaaggacgtg gcggtggacc gcgtcctcgc cgacctggcg cgcgagatcg
     1321 gcgcgcgcgc ctccagcctc accgtgggcc gcgcgtgagc ggctggccga cgtggagcac
     1381 gccccgccgc gcgagcgcgg ggaggcagcg ccgccgtgtg cggcgtggcg gcgcgccgcg
     1441 agcgcgcacg ccctttatca gcgcctaacg aggagcgacg cctatcagcc aagccaagga
     1501 gatccgcgtc aacgaaggca tccgtgtccg ggaagtccgc gtggtgagcg cggacgggga
     1561 gcagctcggg atcatgccca cccagcaggc gctcgagctc gcgcgcaccc gcgagttcga
     1621 cctggtggag gtcgcgccgg aggcgcagcc gccggtgtgc cgcatcatgg acttcgggaa
     1681 gtacaagtac acgcaggccc ggcgtctcaa ggaagcacgg aagaagcaga cgacgatcca
     1741 gatcaaggaa gtgaagatgg ggccgaagac ggagaagcac gacttcgact tcaagctgaa
     1801 gcacgtgcgc cgcttcctgg aggacggcca caaggccaag gtcaccgtgc gcttcaaggg
     1861 ccgcgagatg gcgcacaccg agctcggctg gaagatgctc cagaagatgg tggaggccgt
     1921 ccaggacatc gcgctggtgg agaacaaccc gcgcatggag ggacgcatgc tccacatcat
     1981 gctgagcccg aaggcgcaca actgagggcg aggagagcgg gatgccgaag atgaagacca
     2041 agcggggggc ggccaagcgg ctcaagaaga ccgcctccgg caagctgagg cgggcggggg
     2101 cctggaagca gcacaagttg gagggcaagg atccccaccg gcgccggcgc ctgcgcaaag
     2161 cgaagctgat cacgaaggcc gacgagccgc ggctcacggt cctcgtgccg tacctgtaag
     2221 gcgagacccc aaggagggcg tgcggcaatg ccacgggcaa agggcggatc caagacgcgc
     2281 cagcggcgca agaaggtcct caagaaggcc aagggctacg tcggcggccg gcaccggctg
     2341 tatcgcccgg cgaaggagac ggtgctgcgc gccggcgcgt tcgcgtaccg cgcccggcgt
     2401 cagaagaagc gcctggcacg cgcgctgtgg atcgtccgca tcaacgccgc gtgccgccag
     2461 accggtctgt cgtactcgac gttcatggcc gcgctcagga aggccggtat cctcctcgac
     2521 cgcaaggtgc tggccgagct ggcggtggcc gatccggccg cgttcgcgaa gctcaccgag
     2581 accgcgaagg cgaacgccta acgcgcggtg gcccaccccc gcgtcgacgc catcgcggag
     2641 gaggcgcggg ccgcgatcgc ccgcaccggc tcgtccgccg agctcgagca gctccgcgtc
     2701 cgctacctcg gacgccaggg cgcgctgacg ctgctcctgc gctcgctggg ctcgctgccg
     2761 gccgccgagc gcccgctcgt cggcgccgcc gccaacgagg ccaagcgcgc cctggaggcg
     2821 ctgctcgaca tgcgcctggg cgagatgcgc gaggcggagc ggcgacacga gcgcgcgcgc
     2881 cggcgcctgg acctcacgct gccgggccgc cgtccgcagc tcggcacgct gcacccgctc
     2941 acccgcgtac acgacgagat cgtcgcgatc ttcgtcggcc tcggcttctc cgtcgccgag
     3001 gggcccgagg tcgaggacga ctaccacaac ttcgaggccc tcaacattcc gcgcgaccat
     3061 ccggcgcgcg acatgcagga cacgttctac ctgtccgacg acgtcctgct gcgcacgcac
     3121 acctcgcccg tgcagatccg cacgatgcag gcgcagcggc cgcccgtgcg gatcgtcgtg
     3181 cccggccggg tgtaccgtcg cgacgagctg gatccgtcgc actcgccggt gttccaccag
     3241 gtcgagggcc tggccgtgga tcgccacatc acgatggggg acctcaaggg cacgctggac
     3301 ctgttcgcgc gccagatgtt cgggccccgc tcgagggtcc gcttccggcc gtcgttcttc
     3361 ccgttcaccg agccctccgc ggaggtggac gtcctctgct tcctctgcgg aggcgacggc
     3421 tgccgcgtgt gcaagcaatc cggctggctc gagatcctgg gctcgggcat ggtgcacccc
     3481 cgcgtgctgc agaacgtcgg gtacgatccc gaggaggtca ccggctgggc cttcggcatg
     3541 ggcatcgagc ggatcgccat gctcaagtac ggcgtcgacg acatccggct cttcttcgag
     3601 aacgacctgc gcttcctgag gcagttcgcg tgaagatccc gtaccgctgg atcaaggagt
     3661 tcgtggaggt cgatctcaca ccccagcagg ccgcggagcg gctcaccaac gccggcatcg
     3721 aggtggccgc ggtgatcccg ctcgccccgg ccggcctcgc cggcgtggtc gtcggcgaga
     3781 tcgaggccat cgagcgcgag ctcggcgaca gccgcggtca ccgcgtggtc ctctgccgcg
     3841 tcaccacggg ccgcgagcgc ttcggcgtgg tctgcggcgc gcccaacgtc gccctcgggc
     3901 tgcgcgcggc gttcgcgccg ccgggggcgc gcttgccgga gccggcgggc ggcagccggg
     3961 ccgtcgccgc cgcccgcatc cgcggcgcgg agtcgcaggg aatgctctgc tcggagcgcg
     4021 agctgggcgt cggggaggag cacgaggccg gcctcctggc cttcccggcc gacgcgccgc
     4081 tcggggccga cgtggtgtcc gcgctcgggc tcgacgacca cgtgctcgag gtggaggtca
     4141 cgcccaaccg ccccgactgc ctctcggtgg tcggcgtggc gcgcgagctc gcggcgctga
     4201 ccggcgcgcc gttccggtcg ccggcgacgg cggtgaagga ggtggacgag ccggtgaccg
     4261 ggctcgcgca ggtccgcatc gaggcgcccg acctgtgcca ccgcttcacc gcgcgcgtga
     4321 tcagcggcgt ccgcgtgggg ccctcgccgg cctggctcgc ggcccgcctg cgcgccgtgg
     4381 ggctgcgccc gatcagcaac gtcgtggacg cgacgaacta cgtgatgtgg gaaatcggcc
     4441 agccgctgca cgccttcgac cacgcgcggc tggccgaggc caccatcgtc gtccgtcgcg
     4501 cgcgggccgg cgagcgcttc accacgctcg acggccagga ccgcaccctg agcggggaga
     4561 tgctcgtgat cgccgatccg gcgaaggccg tgggcatcgc gggggtcatg ggcggcgccg
     4621 acacggaggt gaccgatcgt acgacgcgcg tgctgctcga ggccgcctgg ttcctgccgg
     4681 cctcggtgcg gcgcacgtcg cgggcgctcg gcctcgcgac cgatgcggcc taccgcttcg
     4741 agcggggcgc cgacatcgag gctctcgtgg acgcgagcga ccgcgcggcg cagctcatcg
     4801 cggatctcgg cggaggccgc gtggcccgcg ggatggtcga cgtgt
//
LOCUS       DATIAZ010000150         2451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1750024, whole genome shotgun sequence.
ACCESSION   DATIAZ010000150 DATIAZ010000000
VERSION     DATIAZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750024"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..1833)
                     /locus_tag="VLF19_05235"
     CDS             complement(343..1833)
                     /locus_tag="VLF19_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HSE92688.1"
                     /translation="MVSGLATALLVALEPGNLLACFVGAVLGTFVGVLPGLGPAGAMA
                     LVLPLTVKLGPTPGLIMLAGIYYGSMYGGSTTSILVNVPGESASVVTTIDGYRLARRG
                     RAGAALAVAAIASFVAGTVSVVALQLFAPTVARAALAFGPAEYFAFTLLGLVILANLT
                     GRSRVKSLIMIVVGVMLSTVGMDPLGGTVRFAFDVPGAQAGLSFIAVTAGLFGVAEVL
                     ALMAQPESAAAAARVRFRELYPSREELRRAVPPMLRGTVLGFLVGLVPGPAATIASFA
                     SYGVERKVSKHRNELGRGAIEGVAGPEAANNAAAGGGMVPLLSLGLPFTPATAVLLSG
                     MLLSGVTPGPFLLRDHPQVFWGVIGSFYVGNAMLLVLNLPLVGLFARVATVPPRYLMP
                     GVLVLCAVAAFADNNNLFDVWVMVAAGLAGWALRGLGYDVVPLVLGLVLGPLMERSLI
                     QALTIARGDVGGLWASTQAAVMLTAAAMALALPLGAQLIRRSRLAQ"
     gene            complement(1843..2415)
                     /locus_tag="VLF19_05240"
     CDS             complement(1843..2415)
                     /locus_tag="VLF19_05240"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HSE92689.1"
                     /translation="MTSGRDARRDRWSAAGLAVLALGYLYAARQYPLDSLAAPGPGVF
                     PLAAGLALLVLAGCQAIATLRRHWQQEHGSGAGPRSGPGHTSGAGRGSEPTDGLGTMH
                     GAEPRREPGDTSRRGAPLMMAILLVVYAGSVGVLGFLPASCALIVLATRLMGVPGWWR
                     PTLLATGVTAATYLIFAVWLGVPLPSGPLG"
BASE COUNT          408 a          899 c          907 g          237 t
ORIGIN      
        1 gtaattacat gggggccccg agatggcccc caaaccccca gggcggctcg gcacgccccg
       61 gcaaagccgt ggcgcgcctc gatgtcgcgc acggggcccc gagatgggcc cccaaacccc
      121 cagggcggct cggcacgccc cggcaaagcc gtggcgcgcc tcgatgtcgc gcacggggcc
      181 ccgacatggc ctccaagccc ccgggcggct cggcacgccc cggcaaagcc gtggcgcgcc
      241 tcgatgtcgc gcacgggggc gcgagatgcg cctgaacacc agcactcgag gtgccccggc
      301 ccagccgtgg ccctctcgac aacggaccct cgccgaggcg aattactggg cgaggcgcga
      361 gcgccggatc aactgcgccc cgagcggcag cgcgagggcc atggccgccg cggtgagcat
      421 gaccgccgcc tgcgtcgagg cccagaggcc gccgacgtcg ccccgcgcga tcgtgagcgc
      481 ctggatcagc gagcgctcca tgagcgggcc gagcacgagc cccagcacga gcgggacgac
      541 gtcgtagccg agcccacgca acgcccagcc ggcaagcccg gcggcgacca tcacccacac
      601 gtcgaagagg ttgttgttgt cggcgaacgc cgcgaccgcg cagagcacga gcacgcccgg
      661 catgaggtag cgcggcggga ccgtggccac gcgggcgaag aggccgacca gcgggaggtt
      721 cagcaccagg agcatcgcgt tgccgacgta gaagctgccg atgacgcccc agaagacctg
      781 cgggtggtcg cgcagcagga acggcccggg ggtcacgccg ctcagcagca tgccgctcag
      841 cagcactgcc gtcgccggcg tgaacggcag gccgagcgag aggagcggca ccatgccacc
      901 gcccgccgcg gcgttgttgg cggcctcggg cccggccacg ccctcgatgg cgccccggcc
      961 cagctcgttg cgatgcttcg acaccttccg ctcgacgccg tacgaggcga acgacgcgat
     1021 cgtcgccgcg gggccgggga cgagcccgac gaggaagccg agcaccgttc cacgcagcat
     1081 cggcggcacg gcgcgccgca gctcctcgcg gctcggatac agctcgcgga acctcactct
     1141 ggccgccgcc gcggccgact ccggctgcgc catcagcgcg aggacctcgg cgacgccgaa
     1201 gaggcccgcg gtgacggcga tgaagctgag cccggcctgg gcgccgggga cgtcgaaggc
     1261 gaagcgcacg gtaccgccga gtgggtccat gccaaccgtg gacagcatga ccccgacgac
     1321 gatcatgatc agcgacttca ccctcgagcg cccggtaagg ttggcgagga tcacgaggcc
     1381 cagcagcgtg aaggcgaagt actcggccgg cccgaaagcc agcgcggccc gcgccaccgt
     1441 gggcgcgaag agctggaggg ccacgacgct gaccgtgccg gccacgaacg acgcgatggc
     1501 cgcgaccgcc agcgcggcgc ccgcgcgccc gcggcgggcc aggcggtagc cgtcgatcgt
     1561 ggtcacgacg ctggcggact cgccggggac gttgacgagg atcgacgtcg tggagccgcc
     1621 gtacattgac ccgtagtaaa tgccggcgag catgatcaag cccggcgtcg gtcccagctt
     1681 gaccgtgagc ggcagcacca gcgccatcgc gccggccggc ccgagcccgg gcagcacgcc
     1741 cacgaacgtg ccgagcacgg ccccgacgaa gcacgcgagg aggttgccgg gctcgagcgc
     1801 gacgagcagc gcggtcgcga ggccggagac caccgcgcct ccctagccga gcgggcccga
     1861 aggcaggggg acgcccagcc agacggcgaa gatgagatac gtggcggcgg tgacgccggt
     1921 ggcgagcagc gttggccgcc accagcccgg gacgcccatg agacgcgtgg cgagcacgat
     1981 gagagcgcac gaggcgggga ggaagccgag cacacccacg ctcccggcgt agacgacgag
     2041 gaggatggcc atcatcagcg gcgccccccg ccgggaggtg tccccgggct cccgccgggg
     2101 ctccgctccg tgcatcgtgc ccaggccgtc cgttggctcc gagcctcgtc ccgcgcccga
     2161 ggtgtgcccc gggcccgagc gaggccccgc gcccgagcca tgctcctgtt gccaatggcg
     2221 gcgcagcgtg gcgatcgcct gacagcccgc gaggacgagg agggcgagcc cggcggcgag
     2281 gggaaacacg ccgggacccg gggcggccag cgagtcgagg gggtactggc gagcggcata
     2341 gaggtaaccg agggcgagga cggcgaggcc ggccgcgctc catcgatcac ggcgcgcgtc
     2401 gcgacccgac gtcatgccgg cgcgctactg cttcttgagc ccgatctgct c
//
LOCUS       DATIAZ010000151        12452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_829182, whole genome shotgun sequence.
ACCESSION   DATIAZ010000151 DATIAZ010000000
VERSION     DATIAZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829182"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1162)
                     /locus_tag="VLF19_05245"
     CDS             complement(404..1162)
                     /locus_tag="VLF19_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSE92690.1"
                     /translation="MRREFRALRRAPLAARILVATVVVLTAWAAVNWVYQVFRKPTEL
                     FFPVSSALVKTPPETWRQYGPLFRAHATAVITPDLLAALAQVEGAGNPVARTYWRWQL
                     SPNPFELYRPASSAVGMYQITDGTFHEARRYCVHNHAVVEAGAWYDVRSCWFNSLYTR
                     VVPSHAVELTSALLDRGVASTLARLRIAAASPPQKQDLAAVIHLCGAGGGDAYARRGF
                     RVTVDQHCGDHDVGAYLARVNALKRQFGRLAASG"
     gene            1526..1900
                     /locus_tag="VLF19_05250"
     CDS             1526..1900
                     /locus_tag="VLF19_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSE92691.1"
                     /translation="MTLTVEGANHDAALRGVIQRKLGSLESRRRVKAVSARVGFRDEN
                     GPKGGVDIRCALTLELPRRPTLHVEDVAGTHRGAFDAAFESLERRLERELQQGRDRRR
                     RPKKYYVAKRLLAPDAETPFEP"
     gene            2204..2341
                     /locus_tag="VLF19_05255"
     CDS             2204..2341
                     /locus_tag="VLF19_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92692.1"
                     /translation="MEPLRMGLVRAWVEVANDRLTIWRVIPIVPRCREPAARAAGDQA
                     A"
     gene            complement(2357..3787)
                     /locus_tag="VLF19_05260"
     CDS             complement(2357..3787)
                     /locus_tag="VLF19_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSE92693.1"
                     /translation="MGSFPEYERYDALGLALLVRRREISAGELLEAAIERVQARNPKV
                     NAVVLKLYDRAREEAAAGLTDGVFTGVPYLVKDLGASIAGVPTTRGSRFFADSPPAAA
                     DSEHVARLKRAGLVIFGRTSTCELGLSLTCEPQLHGPTRNPWDPERISGGSSGGAAAA
                     VGARMVPMAHASDGFGSIRAPAACCGGVGLKPTRARNTMAPFAGEGLGGLSTEHAVTL
                     TVRDCAALLDATRGAGPGDPYVAPVPARPYLDEVGADVGRLRIAVTSTAPNGAPVDAE
                     PLRVLRETAGLCAELGHAVEQVSPEIDGAAVVPTFLTLAAANTVVNLASHPTRGRPAR
                     QDEVERVTWATGQKGERISAADYVRATQVMHRLGRQMAAFHQHWDVLLTPALAMLPPK
                     LGWIDMTMDDVDEYWRRVFAFSPFTVWFNLTGQPAMVVPIDRSPEGLPVAVQLVARYG
                     DEATLFRLAAQLERARPWIDRRPPVR"
     gene            complement(3791..4750)
                     /locus_tag="VLF19_05265"
     CDS             complement(3791..4750)
                     /locus_tag="VLF19_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HSE92694.1"
                     /translation="MKILYTPLMIVPSIGDGDRARILEAAGPGARLVEERDAARQRAE
                     IPDSDVLFGRVPNDVYLQARKLRYYHSIGAGVDAILSHELVESDVVVASEKGQVGVHL
                     AEHAFALLLGLTRGLHTALRRNDWSLRESIRREQRELYDQTMGVVGLGGTGRDVARRA
                     LGFGMRVLAVDIEDVPPEPGVTLWKADRLHDLLAGSDVVVIALPLTKATHHLFSRDLF
                     RRMRRHAILINVTRGAIVYGDDLLRALEEGLLWGAGLDVTDPEPLPPGHAFWTHPHVI
                     VTPHTAGGSPRRAQRAIDTFVENLKRLRSGHPLLAVIDKRKGY"
     gene            complement(4747..5721)
                     /locus_tag="VLF19_05270"
     CDS             complement(4747..5721)
                     /locus_tag="VLF19_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HSE92695.1"
                     /translation="MASTILFSPPMPPMLMDVARAMVPPGFDLLAADRRAPDFLRALP
                     EAEYWMGFARAGLDREFFATARRLRLVQLISAGYDTIDVEAARRAGVPIANNGGANAI
                     SVAEHTLMLMLAVLKRMVWCHTNVVAGKWRVGGFDERILYELSGKTVGIIGLGRIGKK
                     VARRVQGFDARVQYYDIVRLTEDQEDALGVRFVLLDELLRTSDVVTVHVPLNDVTRGL
                     MSTREFALMKPGAIFVNTCRGPVVDEAALEKALTSKHLGGAGLDVMVDEPPQPGHPLF
                     GLENVVITPHMAGPTWENWPRAFRNGFDNILRVAAGRPPLWVVAELAR"
     gene            5817..7757
                     /locus_tag="VLF19_05275"
     CDS             5817..7757
                     /locus_tag="VLF19_05275"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HSE92696.1"
                     /translation="MPAIALEIQRRAPVLDGRPFGAAGAYEKVAGVLRFAADPDHPLH
                     ASICDLGLAPRSRDGRVEFAGDFYLLKPVDPARGNRRLLLDVPNRGRKVALGMLNSTP
                     RVPDPTTPADFGNGFLMRHGWSVAWVGWQPDVPRQDGLMALDVPAARGVTGLVRCEFR
                     TNARVETLPMADRYHVPYPTVDPSDPEARLTVREHAAAPEQAVPRTAWRFADACHVRL
                     EGGFEPGRIYDLVYRSADPPLVGLGFLAVRDAAAFLRWGTVAEANPCAGALDRAYVFG
                     VSQSGRFLRHLLYLGLTEDERGRDVFDAVVPHVAGARRGEFNMRFGQPSLNALQAVGS
                     LFPFTDGEQVDPLTGERGALLARLRHRGRVPRILTINTAAEYWRGDGSLVHTDVEGKT
                     DLEPPVEARVYLFAGAQHTPGALPPPDADPNTGGKGLQPFNTVDYAPLLRAALVSLDR
                     WVSDGVKPPPSAFPRLADASAVSAESTAGTFGRIPGVRFPDRVVRPGRLDFGRDVERG
                     VVTELPPKAGAPYTTFVSAVDADGNEIAGVRAVELAAPLATFTGWNPRHPTQGAPGDL
                     MSMMGSTLPFAPTRAERARNGDPRASVEERYASRDAYLAAVRAAARQLVAARWMLDED
                     VEPAVERAGRMWDHFRKGLARP"
     gene            7721..8224
                     /locus_tag="VLF19_05280"
     CDS             7721..8224
                     /locus_tag="VLF19_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE92697.1"
                     /translation="MGSLPEGARAALIARAGPEHHAEALALIGAVYAEYGMTFEPDGW
                     DRDLRDIDAQYGDAGGAFWVLLADGRVVGTVAMVPLDPRTVEMKRVYLHREYRGRGLG
                     RALLEHALRWAAAAGYGAVVAWSDVRLIHAHPVYERLGFHRFGERTVDDADRSHEIGF
                     RKVLQPG"
     gene            complement(8303..8725)
                     /locus_tag="VLF19_05285"
     CDS             complement(8303..8725)
                     /locus_tag="VLF19_05285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSE92698.1"
                     /translation="MPADLLAAARDRARANSFWQFLGIEVESAREGWIRLRVPVRDEL
                     RNAPGAPVHGGVYSALVDAAVGGALGTVHGASEGGVGQTTLDLNVSFMAAVTGDAVFA
                     EGTILKRGRTIVFGEARITDATGRLVAAGRATYMVPGR"
     gene            complement(8769..9020)
                     /locus_tag="VLF19_05290"
     CDS             complement(8769..9020)
                     /locus_tag="VLF19_05290"
                     /inference="COORDINATES: protein motif:HMM:NF018744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1344 domain-containing protein"
                     /protein_id="HSE92699.1"
                     /translation="MKKMLGIAVALVLAMSTGALAADVEGKVQSVDISDRAIVLDNGT
                     KLWVAEGVPMDKVKEGATVKASYEERDGKNVITGLEVSE"
     gene            complement(9230..9949)
                     /locus_tag="VLF19_05295"
     CDS             complement(9230..9949)
                     /locus_tag="VLF19_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HSE92700.1"
                     /translation="MRALAMVVATAALVGGCVSAAPDDRSAVMRVITASTVQLRADRD
                     GGGRRTASGVVVAADAARRRSWIVTARHFLDPGTRQKVSVSGVAGRARTVATVSALSD
                     EADLALVEVEDVVLSAVVLKDHAQLGDEVWVVSFPFGKRLTVVSGTVSQIASEDGDVP
                     LEGPVRLVDAQASYGSSGGGIYDAVTGRLLGIIEGYRTARLTLPTTPERTVDVPIPGE
                     TTVLAAPSIRKFLRIVGIERE"
     gene            complement(9946..11310)
                     /locus_tag="VLF19_05300"
     CDS             complement(9946..11310)
                     /locus_tag="VLF19_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE92701.1"
                     /translation="MTAVATVLVADDDPVARDLLVEVLERDGHRARAAAGGEESIRLA
                     EAEVLDLALVDLRMPDVDGLKVLRRLLALEPPVPVIILTAFATIDTAIEAIRAGAYDY
                     LSKPFRIGEIRLVVRRALEARRLARENLEYRRELRERYRVEGLVGQSPGMVALYKLIA
                     RVAALDTTVLIEGETGTGKELVARAIHYASPRAHRPFVVVDCASLPETLVESELFGHE
                     RGAFTGALHARRGLLETADGSTCLLDEVGELPPPLQAKLLRVLQDRIIRRVGGNDPIA
                     VDVRLVAATNRDLRKRVEEGAFREDLYYRLNVVPVRVPALRERLEDVPLLAQHFLRKS
                     SAAAGKTVAGFTAEALALLTAHAWPGNVRELENAVERAVALTSASLVLPDDLPAEVRG
                     ASPPPPVLPQRRMTLEELKSWYVNTVLEETGGNKVRAAEILGIDRRTLYRILDRDAED
                     PEAS"
     gene            complement(11310..>12452)
                     /locus_tag="VLF19_05305"
     CDS             complement(11310..>12452)
                     /locus_tag="VLF19_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92702.1"
                     /translation="LVDAERARYWEVVAPVALDGGVVGAVAAKFSLARADALAARIGT
                     AALVLTAASVVVMGLLMGLAVQVIVNRPLRRFMDTIDRVNGGDATATVTLGTRDELGA
                     LARHFNDMLARIRDFSDELRARVKDATAELEHRYAEVERLNELLFSMQRSLVHAERLA
                     LPGRIMAEVAHEVGTPLHSVSGHLELLRQDLPPAALTGGVRRRLDIVESQLARVTEII
                     AQLLDLTRRTSGEPSPIDLRQLVHECVELVRPGVAAAGLTLDVAALSPVPPVRGHASQ
                     LQQVALNLITNAMDATPSGGRISVAVRTHGGEVELSVADTGRGIPAGEQKRVFEAFFS
                     TKEPGRGSGLGLFISSQIIREHGGRIEIASAEGRGSTFRVLLPATA"
BASE COUNT         1713 a         4485 c         4594 g         1660 t
ORIGIN      
        1 ggcggatacc cgcacgcggc ctcctccctc ggctcgagcc ccgatgcccc tcaggggaag
       61 cggtcgggat gtggcgtgaa tgtgcccgtg gcgccgccac ttgtcaagcg gctgccccag
      121 cggttccgct cgccctgctc catgcgggct ggcggcgggt caccggcatc gggacgttcg
      181 tggcagcgac cgttcgtggc agcgaccgct tctcgccgcg ccggccgtga ctgttctgcg
      241 cggtgcgcac ggacgaagac ccgaccggcg aagacgagtc cggcggcgac ggcactggat
      301 cacgattccc accgagcctt ggcggcacgc gagttggacg atggggctgc cctgaaatac
      361 gcccggcggg cgatgatggc ccggcggccc cgagtttgtc accctagccg gaggccgcca
      421 gtcgaccgaa ctggcgcttc aaggcgttga cccgcgcgag gtaggcgccg acgtcgtggt
      481 cgccgcagtg ctggtcgacg gtcacccgga agccgcgacg ggcgtaagcg tcgccccctc
      541 cggcgccgca gaggtggatg accgcggcca gatcctgttt ctgcggcggc gaggccgcgg
      601 cgatgcgcag tcgcgccagc gtgctcgcga ccccgcggtc gaggagcgcc gacgtcagct
      661 cgacggcgtg actcggcacg acccgggtgt agaggctgtt gaaccagcac gatctgacgt
      721 cgtaccaggc gccggcctcg accaccgcgt ggttgtggac gcagtagcgt ctggcctcgt
      781 ggaacgtccc gtcggtgatc tggtacatgc cgaccgcgct cgacgccggc cggtacagct
      841 cgaagggatt cggcgacagc tgccagcgcc agtacgtccg ggcgaccggg ttacccgcgc
      901 cctcgacctg ggcgagcgcg gccagcaggt cgggcgtgat gacggcagtg gcgtgagcgc
      961 ggaagagcgg cccgtactgc cgccacgtct cgggcggcgt cttcacgagc gcactgctca
     1021 ccggaaagaa cagctcggtc ggcttccgga acacctggta cacccagttc accgcggccc
     1081 aggccgtcag gacgacgacc gtggcgacga ggatccgggc cgccagcggc gcccgtcgca
     1141 gggcccggaa ctcgcgccgc acggatcggg acgcgcggcg caacacccgg gccgtgcgcc
     1201 gcagcgcccg gaaagcatgc cgcagcgttc ggcgcgcgag ccggtgcgac cggctcaggc
     1261 cccgagtggt cagcgcaacc gatttcatac gggtcccgac gactccctcc atactaccgg
     1321 cggcctgggt gaggcagaaa cagcgtctgc gaaacgggac gaggcccgtg atgacagggg
     1381 gccgagggcc agatcggcga ggctccctgg cggctacgcc gggtgggaga ccggcggcga
     1441 agctggtcac cgggtgaaag gattaccgcg ccggccagac agctcgacgc gacgatggca
     1501 cgtcggttgc ttgaacaatc accgcatgac gctcacggtc gaaggcgcga accacgatgc
     1561 cgccctgcgc ggcgtgatcc agcgcaagct cggatcgctc gaaagccgtc gccgcgtgaa
     1621 ggccgtgagc gcgcgcgtcg gcttccggga cgagaacggg ccgaaggggg gcgtcgacat
     1681 ccgctgcgcg ctgacgctgg agctgccgcg gcggccgacc ctgcacgtgg aggacgtcgc
     1741 cggcacgcat cggggcgctt tcgacgccgc gttcgagagc ctcgagcgcc gtctggagcg
     1801 cgagctccag caggggcgcg accggcgccg ccgcccgaag aagtattacg tcgccaagcg
     1861 cctcctcgca ccggacgccg agacaccgtt cgagccctga cggagccgag ggaaatacgg
     1921 gcggcgcaca catgccgcga gccatctgga aggacgcgat cagcttcggg tgaccatccc
     1981 ggttgctctc tactcgcagg accccgcgcg cctccgtgtg gacctcgctc cgacgacgac
     2041 gcgccacggt gcgattgcgg tggccgtcga ccgggcctcg tggacgccgt caagttcacg
     2101 aaggtgacgc cggcggccct gtgggcggag gcaggtccgg atcgcatcgt ctttgtcgtg
     2161 caggtgaccg acccgctcgc cggggctttg gtgggtgtgc gacgtggagc cgctgcggat
     2221 gggcctcgtc cgcgcgtggg tggaggtcgc gaacgacagg ctgaccatct ggcgggtcat
     2281 ccccatcgtg ccgcgctgcc gcgagccggc cgcccgggcc gcaggtgacc aggcggcgtg
     2341 aggccccttc gacgacctag cggacgggcg gccggcggtc gatccacggc cgcgcgcgct
     2401 cgagctgcgc ggccagccgg aagagggtgg cctcgtcgcc gtagcgcgcg acgagctgca
     2461 cggccaccgg cagtccctct ggcgagcggt cgatcggcac caccatcgcc ggctgacccg
     2521 tcaggttgaa ccacacggtg aagggcgaga acgcgaacac ccgacgccag tactcgtcca
     2581 cgtcgtccat cgtcatgtcg atccagccga gcttcggcgg cagcatcgcg agggccggcg
     2641 tcagcagcac gtcccagtgc tggtggaagg ccgccatctg gcgtccgagc cggtgcatga
     2701 cctgtgtggc gcgcacgtag tccgcggcgc tgatgcgctc gcccttctgg ccggtcgccc
     2761 acgtcacgcg ctcgacctcg tcctgacgcg ccggccggcc cctggtcgga tggctcgcga
     2821 ggttcacgac ggtgttggcc gccgcgagcg tgaggaaggt gggcacgacc gcggccccgt
     2881 cgatctcggg gctgacctgc tcgaccgcgt ggccgagctc cgcgcagagc ccggcggtct
     2941 cgcggagcac ccgcaggggc tcggcgtcga ccggcgcgcc gttgggcgcc gtcgaggtga
     3001 cggcgatccg caggcgcccg acgtcggcac cgacctcgtc caggtacggg cgcgcgggga
     3061 ccggggccac gtacgggtcg ccggggccgg cgccgcgggt ggcgtccagg agcgccgcgc
     3121 agtcgcggac ggtgagggtg acggcgtgct cggtcgagag gcctccgagc ccctcgccgg
     3181 cgaacggcgc catggtgttg cgcgcgcgcg tgggcttgag gccgaccccg ccgcagcagg
     3241 ccgccggcgc gcggatggag ccgaagccgt ccgaggcgtg ggccatgggg accatgcgcg
     3301 cgcccacggc cgcggccgcg ccgccgctcg agccgccgga gatgcgttcc gggtcccacg
     3361 ggttacgggt gggaccgtgc agctgcggct cgcacgtgag cgagaggccg agctcgcacg
     3421 tgctggtgcg cccgaagatc acgagccccg cccgcttgag gcgcgcgacg tgctcgctgt
     3481 cggccgccgc cggcggcgag tccgcgaaga accgcgagcc gcgcgtggtc ggcacgccgg
     3541 cgatcgacgc gccgagatcc ttcacgagat acggcacgcc ggtgaagacg ccgtcggtga
     3601 ggccggcggc ggcctcctct cgcgcgcggt cgtagagctt caggacgacg gcgttgacct
     3661 tcgggttgcg cgcctgcacc cgctcgatcg cggcctcgag cagctcgccg gccgagatct
     3721 cgcggcggcg caccaggagg gcgagcccca gcgcatcgta gcgctcgtac tcggggaacg
     3781 agcccatcgg tcagtacccc ttgcgcttgt cgatgacggc gagcagaggg tgacccgagc
     3841 gcagccgctt caggttctcc acgaaggtgt cgatcgcgcg ctgcgcccgc cggggcgagc
     3901 cgcccgcggt gtgcggggtg acgatgacgt gcgggtgggt ccagaaggcg tgccccggcg
     3961 gcagcggctc ggggtcggtc acgtccagtc ccgcgcccca gaggaggcct tcctcgagcg
     4021 cgcggagcag gtcgtcgccg tagacgatgg cgccgcgggt gacgttgatc aggatggcgt
     4081 ggcggcgcat gcggcggaag aggtcgcggg agaagaggtg gtgcgtggcc ttcgtcagcg
     4141 gcagcgcgat gaccaccacg tcggacccgg cgagcaggtc gtggaggcgg tcggccttcc
     4201 agagggtcac gcccggctcg ggcggcacgt cctcgatgtc cacggcgagg acgcgcatgc
     4261 cgaagccgag cgcccgccgg gcgacgtcgc gcccggtgcc gccgagcccg acgacgccca
     4321 tcgtctggtc gtagagctcc cgctgctcgc ggcggatcga ctcgcggagc gaccagtcgt
     4381 tccgccgcag cgccgtgtgc aggccccggg tgagcccgag cagcagcgcg aacgcgtgct
     4441 cggcgaggtg cacgccgacc tgacccttct cgctggccac gacgacgtcg ctctcgacca
     4501 gctcgtgcga gaggatcgcg tccacgccgg cgccgatgga gtggtagtag cgcagcttcc
     4561 gggcctggag atacacgtcg ttgggcacgc ggccgaacag gacgtcggaa tcggggatct
     4621 ccgcgcgctg ccgggcggcg tcgcgctcct cgacgaggcg ggcgccgggg cccgccgcct
     4681 cgaggatgcg ggcccggtcg ccgtcgccga tcgacggcac gatcatgagg ggcgtgtaga
     4741 ggatcttcat cgtgcgagct ccgcgaccac ccagagcggc ggtcgcccgg cggccacccg
     4801 caggatgttg tcgaagccgt tgcggaaggc gcgcggccag ttctcccagg tcgggccggc
     4861 catgtggggc gtgatgacga cgttctcgag cccgaacagc ggatgacccg gctggggcgg
     4921 ctcgtccacc atgacgtcga gcccggcccc gcccaggtgc ttcgaggtga gcgccttctc
     4981 cagcgcggcc tcgtccacca ccggtccgcg gcaggtgttc acgaagatcg cgccgggctt
     5041 catcagggcg aactcccggg tgctcatgag cccgcgggtg acgtcgttga gcggcacgtg
     5101 cacggtcacg acgtccgacg tccgcaacag ctcgtcgagc agcacgaagc gcacgccgag
     5161 cgcgtcctcc tggtcctcgg tcagccgcac gatgtcgtag tactgcacgc gcgcgtcgaa
     5221 gccctgcacg cggcgggcca ccttcttgcc gattcgcccc agcccgatga tgccgaccgt
     5281 cttgcccgac agctcgtaca ggatgcgctc gtcgaagccg cccacccgcc acttgccggc
     5341 gacgacgttg gtgtggcacc acaccatgcg cttgagcacg gccagcatca gcatgagcgt
     5401 gtgctcggcc acggagatgg cgttggcgcc gccgttgttg gcgatgggca cgccggcgcg
     5461 gcgggcggcc tcgacgtcga tcgtgtcgta cccggcgctg atcagctgca ccagcctgag
     5521 gcggcgcgcg gtggcgaaga actcccggtc gaggccggcg cgcgcgaagc ccatccagta
     5581 ctccgcctcg ggcagcgcgc ggaggaagtc gggcgcgcgt cggtcggcgg cgagcaggtc
     5641 gaagccgggc ggcaccatcg cccgggcgac gtccatgagc atcggcggca tcggcggcga
     5701 gaagaggatc gtgctggcca cggcgttctc cttgtcggct ggtatcgccc gccgccggtg
     5761 ggcggcgggc gtggcccctt tatagtagag tcgctggcac cggacaagga ggctccatgc
     5821 ccgccatcgc cctcgagatc cagcgccgcg cgcccgtcct ggacggccgg cccttcggcg
     5881 cggccggcgc ctacgagaag gtcgccggcg tcctgcgctt cgccgccgac cccgaccacc
     5941 ctctgcacgc ctcgatctgc gacctcgggc tggcgccgcg gagccgggac gggcgcgtgg
     6001 agttcgccgg cgacttctat ctcttgaagc cggtagaccc ggcgcgcggc aaccgccgtc
     6061 tgctgctcga cgtcccgaac cgcgggcgga aggtcgcgct cggcatgctc aacagcacgc
     6121 cgcgagtgcc cgacccgaca acgccggcgg acttcggcaa cggcttcctg atgcgtcacg
     6181 gctggagcgt cgcgtgggtc ggctggcagc ccgacgtgcc gcgccaggac ggcctcatgg
     6241 cgctcgacgt gccggcggcg cgcggggtca ccggtctcgt gcgctgcgag ttccgcacca
     6301 acgcgcgcgt cgagacgctg ccgatggccg accgctacca cgtgccgtat ccgaccgtcg
     6361 acccgagcga tcccgaggcg cgcctgaccg tgcgcgagca cgccgctgcg cccgagcaag
     6421 ccgtgccgcg gacggcgtgg cgcttcgccg atgcgtgtca cgtgcggctc gagggcggct
     6481 tcgagcccgg caggatctac gatctcgtct atcgctccgc ggacccgccg ctcgtgggcc
     6541 tcggcttcct ggccgtccgc gacgcggcgg cattcttgag gtggggaacg gtggccgagg
     6601 ccaacccgtg cgcgggagct ctggatcgcg cctacgtgtt cggcgtctcg cagagcggcc
     6661 gcttcctgcg ccatctgctc tatctcggac tgaccgagga cgagcggggc agggacgtct
     6721 tcgacgccgt cgtcccgcac gtcgccggcg cccggcgggg cgagttcaac atgcggttcg
     6781 gccagccctc gctcaacgcg ctgcaggccg tcggcagcct cttcccgttc accgacggcg
     6841 agcaggtcga tccgctcacc ggtgagcgcg gcgccctcct cgcccggctg cggcaccgag
     6901 gccgcgtccc gaggatcctc acgatcaaca ccgcggccga gtactggcgc ggcgacggct
     6961 cgctggtgca caccgacgtg gaaggcaaga ccgacctcga gcccccggtc gaggctcgcg
     7021 tgtacctttt tgccggcgcg cagcacacgc cgggagcgct gccgccaccg gacgcggacc
     7081 cgaataccgg cggcaagggc ttgcagccgt tcaacaccgt ggactacgct ccgctgctgc
     7141 gagccgccct ggtgagcctc gaccgctggg tgagcgacgg cgtgaagccg ccgccgagcg
     7201 ccttcccgcg cctcgccgac gcctcggccg tcagcgccga atcgacggcg gggacgttcg
     7261 gcaggattcc cggcgtgcgg ttccccgacc gcgtcgttcg cccgggccgg ctcgatttcg
     7321 ggcgggacgt cgagcgcggc gtcgtcaccg agctgccgcc caaggccggc gcgccgtaca
     7381 cgacgttcgt gtccgcggtg gacgccgacg gcaacgagat cgcgggcgtg cgagccgtgg
     7441 agctcgcggc accgctcgcc accttcaccg gctggaaccc gcgccatccc acgcagggcg
     7501 cgccggggga cctcatgtcc atgatgggct cgacgctgcc ctttgcgccg acacgcgccg
     7561 agcgcgcgcg gaacggcgat ccgcgcgcct cggtggagga gcgctacgcg tcccgcgacg
     7621 cgtacctcgc ggccgtgcgc gccgccgcgc ggcagctcgt cgccgcgcgc tggatgctcg
     7681 acgaggacgt ggagccggcc gtcgagcgcg cgggccgcat gtgggatcac ttccggaagg
     7741 ggctcgcgcg gccctgatcg cacgcgccgg gcccgagcac cacgccgagg cgctcgccct
     7801 catcggcgcg gtctacgccg agtacgggat gaccttcgag cccgacggct gggaccgcga
     7861 cctccgcgac atcgacgcgc agtacggcga cgccgggggg gcgttctggg tgctcctcgc
     7921 cgacgggcgc gtcgtgggca cggtggccat ggtgccgctg gacccgcgga cggtcgagat
     7981 gaagcgagtg tacctgcacc gcgagtaccg ggggcgcggg ctcggccgcg ccctgctgga
     8041 gcacgcgctg cgctgggccg ccgccgccgg ctacggcgcc gtcgtcgcct ggtccgacgt
     8101 ccgcctgatc cacgcgcacc ccgtgtacga acgcctgggc ttccaccgct ttggcgagcg
     8161 tacggtcgac gacgccgacc gcagccacga gatcggcttc cgcaaagtcc tgcagccggg
     8221 gtgacgccac tcgggcgccg gctcggccgg cccgccgtcg ggcccacccc gcccccgcgc
     8281 gagacgacga cggccccggg gactagcgcc cggggaccat gtaggtcgcg cgtccggcgg
     8341 cgacgagacg tccggtcgcg tcggtgatcc gcgcctcgcc gaagacgatc gtccggcccc
     8401 gcttcaagat cgtgccctcg gcgaacacgg cgtcaccggt gacggccgcc atgaaggaca
     8461 cgttcaggtc gagcgtcgtc tgtcccacgc cgccctcgga cgctccgtgc accgtgccga
     8521 gcgcgccgcc gacggcggcg tccacgagtg cggagtacac gccgccgtgc accggcgccc
     8581 cgggcgcgtt gcgcaactcg tcgcgcacgg gcacgcgcag gcggatccag ccctcgcgcg
     8641 cgctctccac ctcgatgccg aggaactgcc agaagctgtt ggcccgcgcg cggtcgcgcg
     8701 cggcggcgag caggtcggcc ggcatggcgc cccagtatag cggacgccac gccggcccgc
     8761 gggcgcctct actcggagac ctcgaggccg gtgatgacgt tcttgccgtc acgctcctcg
     8821 tacgaggcct tcacggtagc cccctccttc accttgtcca tcgggacacc ctcggcgacc
     8881 cagagcttcg tcccgttgtc gaggacgatc gcgcggtcgg agatgtccac ggactggacc
     8941 ttgccctcga catccgcggc caacgcgccg gtggacatcg cgagcacgag cgctacggcg
     9001 ataccgagca tcttcttcat cctcagcctc ctgtcgcgga atgtacgacg cgcacgaggg
     9061 aggcaacgcg catgccaacc agcgtgtcgg cggacgggtg tcgcggcccg cgagattcga
     9121 gccaccccgg agagaccgcg ggtggtccag tgtggcgcga tggtacgcga ctggggcgga
     9181 tcgcctcaga ccaccctcgg ggtttcgggg ccgcccgagc cccatgtgtt cactcgcgct
     9241 cgatccccac gatcctgagg aacttgcgga tggacggtgc cgccagcact gtggtctctc
     9301 cggggatcgg gacgtccacg gttcgctcgg gcgtcgtggg tagcgtgagc cgcgcggtcc
     9361 ggtagccttc gatgatgccg agcagccggc ccgtgaccgc gtcgtagatg cccccgccgc
     9421 tcgagccgta gctcgcctgg gcatcgacca gtcgcacggg gccctcgagg gggacgtcgc
     9481 cgtcctcgga ggcgatctga ctgacggtcc cgctcaccac cgtcaggcgc tttccgaagg
     9541 ggaaggagac gacccagacc tcgtcgccga gttgcgcgtg gtccttgagg acgaccgcgg
     9601 acaggaccac gtcctcgacc tccacgaggg ccaggtcggc ttcgtcgctg agcgcggaca
     9661 ccgtggccac cgtgcgggcc ctgccggcca cgccgctcac ggacaccttc tggcgcgtgc
     9721 cggggtcgag gaagtgacgc gcggtcacga tccacgaccg acggcgggcg gcgtcggccg
     9781 ccaccacgac cccggacgcc gtccgacgcc cgccgccgtc gcggtccgcc cggagctgta
     9841 cggtcgaggc ggtgatgacg cgcatcacgg cgctgcggtc gtccggcgcc gccgacacgc
     9901 agccgccgac cagcgcggcc gtcgcgacca ccatcgccag cgctctcacg acgcctcggg
     9961 gtcctcggcg tcgcgatcga ggatgcggta gagcgtccgc cggtcgatgc cgaggatctc
    10021 ggccgcgcgc accttgttgc cccccgtctc ctcgaggacg gtgttcacat accagctctt
    10081 cagctcctcg agcgtcatcc ggcgctgcgg gagcaccggc ggcggcggcg acgcgccgcg
    10141 cacctcggcg ggcaggtcgt ccggcaggac gagcgaggcc gaggtcagcg cgaccgcgcg
    10201 ctcgacggcg ttctcgagct cgcgcacgtt cccgggccag gcgtgcgccg tgagcagggc
    10261 gagcgcctcg gccgtgaagc cggccacggt cttgccggcc gcggcggagg acttgcggag
    10321 aaagtgctgg gccagcagcg gcacgtcctc gagccgctcg cggagggccg gaacgcggac
    10381 gggcacgacg ttcagccggt agtacaggtc ctcgcggaag gcgccctcct cgacgcgctt
    10441 gcggagatcg cggttcgtgg cggcgacgag acgcacgtcc acggcgatcg ggtcgttgcc
    10501 gccgacgcgc cggatgatgc ggtcctggag cacgcggagg agcttggcct ggagcggagg
    10561 cggcagctcg ccgacctcgt cgagcaggca ggtgctgccg tcggccgtct cgagcaggcc
    10621 gcgccgcgcg tgcagcgcgc cggtgaaggc gccgcgctcg tggccgaaca gctccgactc
    10681 caccagcgtc tcgggcagcg aggcgcagtc cacgacgacg aaggggcggt gcgcgcgcgg
    10741 gctcgcgtag tggatagccc gggcgaccag ctccttgccg gtgcccgtct cgccctcgat
    10801 cagcacggtc gtgtcgagcg cggcgacgcg ggcgatgagc ttgtagagcg ccaccatgcc
    10861 gggcgactgg cccaccagcc cctcgacccg gtagcgctcg cgcagctcgc gccggtattc
    10921 gaggttttcg cgggccagac gccgggcctc gagcgcgcgg cgaaccacga gccggatctc
    10981 cccgatgcgg aacggcttcg acaggtaatc gtaggcgccg gcgcggatcg cctcgatcgc
    11041 ggtatcgatg gtggcaaacg ccgtgaggat gatcaccggc acgggcggct cgagcgcgag
    11101 cagtcgccgg aggaccttca gcccgtccac gtcgggcatg cggaggtcca cgagggcgag
    11161 gtcgagcacc tcggcctcgg cgagacggat ggactcctcg ccgccggccg ccgcgcgcgc
    11221 gcggtgaccg tcgcgctcca gcacctcgac gagcaggtcc cgggccacgg ggtcgtcgtc
    11281 ggcgacgagc acggtggcga cggcggtcat caggcggtgg cggggagcag gacgcggaag
    11341 gtgctcccgc ggccctccgc gctggcgatc tcgatacgac cgccgtgctc ccggatgatc
    11401 tgggacgaga tgaagaggcc cagaccgctg ccgcgcccgg gctccttggt ggagaagaac
    11461 gcctcgaaca cccgtttctg ctcgcccgcc gggatgccgc gcccggtgtc ggcgacggac
    11521 agctccacct cgccgccgtg cgtccggacg gcgacggaga tgcggccgcc cgagggggtg
    11581 gcgtccatgg cgttggtgat caggttcagc gccacctgct ggagctggct cgcgtggcca
    11641 cgcaccgggg gcaccggcga gagggcggcg acgtcgagcg tgaggccggc cgccgccaca
    11701 cccggccgca ccagctcgac gcactcgtgc acgagctgcc ggaggtcgat cggcgacggc
    11761 tcgcccgacg tgcgccgcgt gaggtcgagg agctgggcga tgatctcggt cacgcgcgcg
    11821 agctggctct ccacgatgtc cagccgccgg cggacgccgc ccgtgagcgc cgccggcggc
    11881 aggtcctggc gcagcagctc caggtggccg ctcaccgagt gcagcggcgt gccgacctcg
    11941 tgggccacct cggccatgat gcgcccgggt agcgcgagcc gctcggcgtg gacgaggctg
    12001 cgctgcatcg agaacagcag ctcgttgagc cgctccacct cggcataccg atgctccagc
    12061 tcggcggtgg cgtccttcac acgcgcccgg agctcgtcgc tgaagtcgcg gatgcgggcc
    12121 agcatgtcgt tgaagtgccg cgccagggcg ccgagctcgt cgcgcgtgcc gagcgtcacg
    12181 gtggccgtcg cgtcgccgcc gttcacgcgg tcgatcgtgt ccatgaaccg gcgcagcgga
    12241 cggttcacga tcacctggac ggcgaggccc atcaacaggc ccatgaccac gaccgaggcc
    12301 gcggtgagga ccagcgcggc ggtgccgatg cgcgccgcca gggcgtcggc gcgcgccagc
    12361 gagaacttcg cggcgacggc gcccaccacg ccgccgtcga gggcgacggg ggcgacgacc
    12421 tcccagtacc gcgcgcgctc ggcgtcgacg ag
//
LOCUS       DATIAZ010000152         7918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_959649, whole genome shotgun sequence.
ACCESSION   DATIAZ010000152 DATIAZ010000000
VERSION     DATIAZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959649"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VLF19_05310"
     CDS             <1..238
                     /locus_tag="VLF19_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92703.1"
                     /translation="IPIVTIVGIELGTLLGGAVITETIFAWPGVGRLSVQAIYNRDYP
                     VVQASVFLLASTFIVVNIVVDMVYTYLDPRIRLR"
     gene            335..1228
                     /locus_tag="VLF19_05315"
     CDS             335..1228
                     /locus_tag="VLF19_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE92704.1"
                     /translation="MPVALPARRSTVGSGWAAFARRLMRRRTALFGLAVVASVVTIAA
                     AAPLLASFDPVEQRIGDRLKPPGWRDEAGRLHVLGTDHLGRDLLARVIFGARPALLVG
                     VAAVLISGIIGMVTGLLAGYFGGRVDDVLMRLGDVQLAFPFILLAIAVIGVLGPSLPV
                     IIVVIGVSSWVIYARVVRGTVLSLREREFVQAARALGARDGRILARHILPNAFTPWLV
                     VATLDMARVIVIESALSFLGLGVQPPAPTWGGMLADGRVYLTTSWWLATFPGLAIVVT
                     VLGINLFGDGLRDTLDPRLKV"
     gene            complement(1275..3080)
                     /locus_tag="VLF19_05320"
     CDS             complement(1275..3080)
                     /locus_tag="VLF19_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSE92705.1"
                     /translation="MTALSTALVTAGTGLTVLVWAVRARWFARLHAGFLVVLMVTLAA
                     VGLAAASLYGLYATETGRVVLLDAVVRGMQNLGTVVEQEIHEEVERARRRLARLGEEI
                     TPDTARARPREVIETLHGILRFDVRFLQIAVLDDQGAPVVSASSVDGTEPIDRVAVAW
                     SLEGKPFVSQPQVSPVFRKYVLVLAVPIHAPDRGVRGAITARWDLQEDLRSLLDTTRF
                     NRTGYTALTDHRGRILAHPDAARVGEDAGTQPAVMQALQGRSGWLVAPAEGGPERLFA
                     YRPVASPATIEPKPWILLTEEAVDDALAPVHALAQRFALALAVVVIGCVALAALIAPA
                     IRRPVADLLAFARRLGQGALDARMALEGRDEVGRLAAALNEMAAGLQERERVKEIFGR
                     YVTTQVSEEVLRGELRLGGERRRVTLLFSDIRNFTTMSEGMAPEEVVGFLNDYFTEMV
                     EAVFEQHGVLDKFIGDGIMATFGALDRSPDHPARAARAALRMQALLAKINGERAMTGK
                     PPFGIGIGIHTDEVVVGNIGSRRRMEYTAIGDGVNTCARVEAANKELGTTILVTETTC
                     AEIRDAFDCRPMPDLPLKGKSKVPRLFELVSLKSR"
     gene            complement(3152..3730)
                     /locus_tag="VLF19_05325"
     CDS             complement(3152..3730)
                     /locus_tag="VLF19_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HSE92706.1"
                     /translation="MAPIYWLPPLAWMAVIFVLSTDVGSSEHTRSWLEPVFRAVRPWA
                     TDVQVRAVHAVVRKAAHVIEYAILAVLWFRAFARGRAWPPAKAASAAFGLAAAWAVVD
                     EAHQAFVASRGAAAGDVLIDTIGAAAAALVARFGWRAAARGATTALLWTAALGGAGAL
                     LLNAAAGVPSGALWLTAPASALLLARRRWRAR"
     gene            3757..6120
                     /locus_tag="VLF19_05330"
     CDS             3757..6120
                     /locus_tag="VLF19_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSE92707.1"
                     /translation="MLGEILDDLLTSPSTGPCITTVKQFPARPPVLVPFPSSLDPRLA
                     VALRRRGIAELYSHQARAWELIDKGHHVVVVTPTASGKTLCYNLPVLQALLGQPDARV
                     LYLFPTKALAQDQLAELEELAKQLPELRMFTYDGDTPQDARRAVRARANLVLSNPDML
                     HSGILPHHTKWAVLFQNLRYVVIDELHAYRGVFGSHVANVIRRLRRLCRHYGSAPQFI
                     MASATIANPRELAERVTGESVEQIAESGAPTGEKVFLCYNPPVVNPELGIRAPYLGEA
                     ARLAVRFLREKIPTIVFAQSRLATEVLLTAVKAGVADKTGDAGIVRGYRGGYLPTRRR
                     AVEQGLRSGEVLGVVSTNALELGVDIGHLDVAVLAGYPGTIASLWQQSGRAGRRTGAS
                     AAIMVATSAPLDQFMVAHPDYLFGTPPEHARINPDNPFILANHLKCAVFELPFAPSAA
                     DGGVERFGALDVRRYLAALEDEGLLHSAGGRWHWASETYPADHMSLRTVTTDNFVVID
                     TTARDHQQTKRRQIIAEVDWGSAFATIHPKAIYLVESESYEVQELRFREDEEKVAYVK
                     RVNVDYFTDAISAKGVWILRRLADRGQAAYLAEQGEVLVAEKVVGFKKIKMGTLENVG
                     SGEVDLPQQEMQTTSAWITVPPATLGRVSQSREELIDGLRAVTYLLHHLAPIFLLCDI
                     RDLGSWLGDTTPAETGIVATRESTKQRLMSVERFNPTIYLYDSHAGGIGLAERIFDVL
                     PDLLRRGLDTLEACPCRWGCPSCVGPVNEVGRRAKATAAALLRELAG"
     gene            6104..7378
                     /locus_tag="VLF19_05335"
     CDS             6104..7378
                     /locus_tag="VLF19_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSE92708.1"
                     /translation="MSSPADAPDRTSTLDDLRRVIRRIEGRRPTRPAPEAVEQVVGGE
                     LQETTAGPILVVRHEYPLGHAHGRVALARALDVPPPVLELIARAKEAPTRARGLLFLD
                     TETTGLAGGTGTYAFLVGAGFVEADRFVVVQYFMRDFDEEPALLAALDPLLARATGLV
                     TFNGGAFDVPLLETRFLMARRRWPTLVAHVDLLRPARRVWSARFDDCRLATLEREVLG
                     LLREDDVPGFMIPALYFDWLRYRRAAPLARVFRHNRDDVLSLAALLGWFTDALAGGAA
                     ALAPWEIAGIGRLWERVDIERAAACYRDAIAAGLDGRDGHRVRLRLALWEKRQARWDA
                     ACTLWQAAARAAAFDPQPWEELAKFHEHRRRDFAAARAVVLQALDLARNTGAPPRVLA
                     AFAYRLARLERRLGREGRAEPGHSFLDPVAPT"
     gene            7535..7660
                     /locus_tag="VLF19_05340"
     CDS             7535..7660
                     /locus_tag="VLF19_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92709.1"
                     /translation="MATIAAFAMCESVAALGLVLSLMWGRQVDVLLLVASRSWVR"
     gene            complement(7726..>7918)
                     /locus_tag="VLF19_05345"
     CDS             complement(7726..>7918)
                     /locus_tag="VLF19_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HSE92710.1"
                     /translation="VPADEGEAVILRRYFRGSENVYCLGLASGHRVHSSQPSSAAFAT
                     GQRVRPEAHVLHVVTFPAD"
BASE COUNT         1071 a         2892 c         2813 g         1142 t
ORIGIN      
        1 catccccatc gtgaccatcg tcggcatcga gctcggcacg ctgctcggcg gtgcggtcat
       61 caccgagacg atcttcgcct ggccgggcgt cggccggctg agcgtgcaag cgatctacaa
      121 ccgggactac ccggtggtgc aggcctcggt gttcctgctc gcctccacgt tcatcgtcgt
      181 caacatcgtc gtcgatatgg tctacacgta cctcgacccg cggatccggc tgcgatgatg
      241 ccccgggctc ccgggcgaag ttccctgcgg ttcgagccag gggggcggcc cccatgacca
      301 cgacgcccgg gcggcgcgaa tgagggtgga ggccgtgccg gtggcgctgc ccgcgcggcg
      361 ctcgacggta ggctccggat gggcggcgtt cgcgcgtcgt ctgatgcgcc ggcgcaccgc
      421 gctgttcggt ctcgccgtcg tcgcctccgt cgtcacgatc gcggccgcgg ccccgttgct
      481 cgcctccttc gaccccgtcg agcagcgcat cggcgaccgc ctcaagcccc cgggctggcg
      541 cgacgaggcc ggccgcctgc acgtgctcgg caccgaccac ctcggccggg acctcctcgc
      601 ccgcgtgatc ttcggcgcgc gaccggcgct gctggtgggg gtcgcggcgg tgctgatctc
      661 cgggatcatc ggcatggtca ccggcctgct cgccggctac ttcggcgggc gcgtcgacga
      721 cgtgctgatg cggctcggcg acgttcagct cgcctttccg ttcatcctgc tggccatcgc
      781 ggtgatcggg gtcctgggcc cgagcttgcc cgtgatcatc gtcgtgatcg gggtgtcgag
      841 ctgggtgatc tacgcacgcg tcgtgcgcgg caccgtgctg tccttgcgcg agcgcgaatt
      901 cgtgcaggcg gcgcgggcgc tcggcgcgcg cgacgggcgg attctcgccc ggcacatcct
      961 gcccaacgcc ttcacaccgt ggctggtcgt ggccacgctc gacatggcgc gagtgatcgt
     1021 gatcgagtcg gccctgtcgt tcctggggct cggcgtccag ccgcccgcac cgacgtgggg
     1081 cggcatgctc gccgacggcc gggtctacct gacgacgtcg tggtggctgg cgaccttccc
     1141 cggactggcg atcgtggtca ccgtgctcgg catcaacctg ttcggcgacg gtctccgcga
     1201 cacgctggat ccgcggctga aggtttgacg ctccgcgcgc gccggccaag cccggcgcgc
     1261 ctcgacatcc tgcgtcagcg ggacttgagg ctgacgagct cgaacaggcg cgggaccttg
     1321 ctcttgccct tgaggggcag gtcgggcatg ggccggcagt cgaaagcgtc acggatctcg
     1381 gcgcaggtcg tctcggtgac gaggatcgtc gtgccgagct ccttgttcgc ggcctcgacc
     1441 cgcgcgcacg tgttgacccc gtccccgatc gccgtgtact ccatccggcg ccgcgagccg
     1501 atgttgccga cgaccacctc gtcggtgtgg ataccgatcc cgatgccgaa cggcggcttg
     1561 ccggtcatcg cccgctcacc gttgatcttc gccaggagcg cctgcatgcg gagcgcggcc
     1621 cgcgccgccc gcgccgggtg atccggcgac cggtccagcg cgccgaaggt ggccatgatc
     1681 ccgtcgccga tgaacttgtc gagcacgccg tgctgctcga agaccgcttc caccatctcg
     1741 gtgaaatagt cgttgaggaa gcccacgacc tcctcggggg ccatgccctc ggacatcgtc
     1801 gtgaagttcc ggatatccga gaacagcagg gtgacccgcc gacgctcgcc gccgagccgc
     1861 agttcgcccc ggagcacctc ctcgctcacc tgcgtcgtca cgtaccgtcc gaagatctcc
     1921 ttcacgcgct cgcgctcctg caggccggcg gccatctcgt tgagcgccgc ggcgagacgg
     1981 cccacctcgt cccggccctc gagcgccatc cgcgcgtcga gcgcgccctg gccgaggcgg
     2041 cgggcgaagg cgaggagatc ggccacggga cggcggatcg cgggtgcgat cagggcggcg
     2101 agcgccacgc agccgatcac gacgaccgcg agcgcgagcg cgaaccgctg cgcgagcgcg
     2161 tgcacggggg cgagcgcgtc gtcgacggcc tcttcggtga gcaggatcca gggcttgggc
     2221 tcgatcgtgg cggggctggc gacggggcgg taggcgaaga ggcgttccgg gccgccctcg
     2281 gcgggcgcca ccaaccatcc cgaacggccc tgcagggcct gcatcactgc gggttgcgtg
     2341 ccggcgtcct cgcccacgcg cgccgcatcc gggtgcgcga ggatccgacc ccgatggtcc
     2401 gtcagcgcgg tgtagccggt ccggttgaag cgcgtcgtgt cgagcaggct gcgcaggtcc
     2461 tcctggaggt cccagcgcgc ggtgatcgcg ccccgcaccc cgcggtcggg cgcgtggatc
     2521 gggaccgcga ggacgagcac gtacttccgg aacacgggtg acacctgggg ctgggagacg
     2581 aacggcttgc cctcgaggct ccaggccacg gcgacacggt cgatcggctc ggtcccgtcg
     2641 acgctgctcg ccgagacgac gggcgccccc tggtcgtcga ggacggcgat ctgcaggaag
     2701 cggacgtcga atctcaggat gccgtggagc gtctcgatca cctcgcgcgg ccgcgcgcgc
     2761 gcggtgtcgg gtgtgatctc ctcgccgagc cgggcgagcc gccggcgcgc tctctcgacc
     2821 tcctcgtgga tctcctgctc gacgacggtg ccgaggttct gcatgccccg cacgacggcg
     2881 tcgagcagaa cgacgcggcc cgtctccgtg gcgtagaggc cgtagagcga ggctgcggcg
     2941 aggcccaccg cggccagcgt gaccatcagc accacgagga acccggcgtg cagacgggcg
     3001 aaccagcggg cccggaccgc ccacaccagc acggtgaggc cggtgccggc cgtcacgagg
     3061 gccgtggaga gcgcggtcat cgcctcttca ctaaagcgca ctcggccgga ggggggaagc
     3121 gaaatcgggc caggtcgccg gccggaggcc ttcagcgggc gcgccaccgg cgccgcgcga
     3181 gcaggagcgc ggatgccggc gcggtgagcc agagggcacc cgacggcacg ccggccgccg
     3241 cgttcagcag gagcgcgccg gcgccgccga gcgcggcggt ccagaggaga gccgtcgtgg
     3301 ccccccgcgc ggcggcccgc cagccgaagc gggccacgag cgccgccgcc gccgcgccga
     3361 tcgtgtcgat gaggacgtca cccgcagcgg cccctcgcga ggcgacgaag gcctgatggg
     3421 cttcgtcgac gaccgcccac gccgccgcca gaccgaaggc cgcagaggcc gccttcgcgg
     3481 ggggccaggc ccggccgcgc gcgaacgcgc gaaaccagag cacggcgagg attgcgtatt
     3541 cgatgacgtg cgcggccttg cggacgacgg cgtgcacggc cctgacctgg acatcggtcg
     3601 cccacggccg cacggcacgg aacacgggct cgagccagga ccgggtgtgc tccgagctgc
     3661 ccacgtcggt ggagagcacg aagatcacgg ccatccacgc cagcggcggc agccagtaga
     3721 tgggtgccat cgaggtccta tgctacagtg gccgcgatgc tcggcgagat cctcgacgat
     3781 ctcctcacgt cgccgtcgac cgggccgtgc atcacgaccg tcaagcaatt cccggcgcgc
     3841 ccgccggtcc tcgtcccgtt cccctcgtcg ctcgatcctc gcctcgccgt ggcgctgcgg
     3901 cgtcgcggca tcgccgagct gtattcgcac caggcgcgcg cgtgggagct gatcgacaag
     3961 ggccaccacg tcgtcgtcgt gacgccgacg gcctcgggca agacgctctg ctacaacctg
     4021 cccgtgctgc aggcgctgct gggccagccc gacgcgcgcg tgctctacct ctttccgacg
     4081 aaggcgctgg cgcaggacca gctcgccgag ctcgaggagc tggccaagca gctgcccgag
     4141 ctccggatgt tcacctacga cggcgacacg cctcaggacg ccaggcgcgc ggtccgtgcc
     4201 cgagcgaacc tcgtcctcag caatcccgac atgctgcact ccgggatcct gccgcaccac
     4261 acgaagtggg cggtgctgtt ccagaacctt cgctatgtcg tcatcgacga gctgcacgcg
     4321 tatcgcggcg tcttcggcag tcacgtggcc aacgtcatcc gccggctccg gcggctctgc
     4381 cggcactacg gctcggcgcc acagttcatc atggcctcgg cgaccatcgc caacccgcgc
     4441 gagctcgccg agcgggtcac cggcgagagc gtcgagcaga tcgccgagag cggcgcgccg
     4501 accggcgaga aggtgttcct ctgctacaac ccgccggtgg tgaaccctga gctcggcatc
     4561 cgcgccccct acctcggcga ggccgcgcgg ctggccgtcc gcttcctccg ggagaagatc
     4621 ccgaccatcg tgttcgccca gagccggctc gcgacggagg tgctgctgac cgcggtcaag
     4681 gccggcgtcg ccgacaagac gggcgatgcg ggcatcgtcc gcgggtaccg cggcggctac
     4741 ctgcccacgc ggcggcgcgc cgtcgagcag ggacttcggt cgggcgaggt gctcggcgtc
     4801 gtctcgacca acgcgctcga gctgggcgtg gatatcggcc acctggacgt cgcggtgctt
     4861 gccggctacc ccgggacgat cgcctcgctg tggcagcagt ccggccgcgc cggccggcgc
     4921 accggcgcct cggccgcgat catggtggcc accagcgctc cgctcgatca attcatggtc
     4981 gcgcaccccg attacctctt cggcactccg cccgagcacg cgcggatcaa tcccgacaac
     5041 cccttcatcc tggccaacca cctcaagtgc gcagtgttcg agctaccgtt cgccccgagc
     5101 gccgcagacg gtggcgtcga gcggttcggc gccctcgacg tgcgccgcta cctggccgcg
     5161 ctcgaggacg agggcttgct gcacagcgcc ggcggccggt ggcactgggc ctcggagacc
     5221 tatcccgccg accacatgtc gctgcgcacc gtgaccaccg acaacttcgt ggtcatcgac
     5281 acgacggcgc gcgaccacca gcagacgaag cggcggcaga tcatcgccga ggtcgactgg
     5341 gggagcgcct tcgcgacgat ccaccccaag gcgatctatc tcgtcgagag cgagtcgtac
     5401 gaggtgcagg agctgcgctt ccgcgaggac gaggagaagg tcgcgtacgt caagcgcgtg
     5461 aacgtcgact acttcaccga cgccatcagc gccaaaggcg tgtggatcct ccgccggctc
     5521 gccgatcgcg gtcaggctgc gtacctggcc gagcagggcg aggtcctggt cgccgagaag
     5581 gtcgtgggct tcaagaagat caagatgggc acgctcgaga acgtgggttc gggcgaggtc
     5641 gacctacccc agcaggagat gcagaccacc agcgcgtgga tcaccgtgcc gccggcgacg
     5701 ctcgggcgcg tgagccaatc gcgcgaggag ctgatcgacg ggttgcgcgc ggtgacgtac
     5761 ctgctgcatc acctggcgcc gatcttcctg ctctgcgaca tccgcgacct cggctcgtgg
     5821 ctcggcgaca cgacgccggc cgagacgggg atcgtggcca cccgcgaatc gaccaagcag
     5881 cggctcatga gcgtggagcg cttcaatccg acgatctatc tctacgattc ccacgccggc
     5941 ggcatcggcc tcgccgagcg catcttcgac gtgctgcccg acctgctccg ccgcgggctc
     6001 gacacgctcg aggcctgccc gtgccggtgg ggctgcccgt cctgcgtggg ccccgtgaac
     6061 gaggtggggc gccgcgccaa ggcgacggcg gccgcgctgc tccgtgagct cgccggctga
     6121 cgctcccgac cgcacgagca cgctcgacga cctccgcagg gtcatccgga ggatcgaggg
     6181 ccggcggccg acgcggccgg cgccggaagc cgtcgagcaa gtggtgggcg gcgagctcca
     6241 ggagacgacc gccggcccga tcctcgtcgt gcggcacgag tacccgctcg gccacgcgca
     6301 tggccgggta gcgctggcgc gcgctctcga cgtcccgccg cccgttctcg agctgatcgc
     6361 gcgcgccaag gaggcgccca cccgggctcg cggcctgctc ttcctcgaca ccgagacgac
     6421 cgggctggcc ggcggcacgg gaacctacgc gtttctcgtc ggcgccggct tcgtggaagc
     6481 cgaccggttc gtcgtggtcc agtacttcat gcgggacttc gacgaggagc cggccctgct
     6541 ggccgcgctc gatccgctgc tcgcgcgggc cacgggcctc gtgaccttca acggcggcgc
     6601 cttcgacgtg ccgctgctcg agacgcgctt cctcatggcg cgccggcgct ggccgacgct
     6661 ggtcgcccac gtggacctgc tgcgtcccgc gcggcgagtc tggtcggcgc gcttcgacga
     6721 ctgccggctg gccacgctcg agcgtgaggt cctcgggctc ctgcgcgagg acgacgtgcc
     6781 cggcttcatg atccctgcgc tctacttcga ctggctgcgc tatcgccggg cggcgccgct
     6841 tgcgcgcgtc ttccgacaca accgcgacga cgtgctgtcg ctggccgcgc tgctcggctg
     6901 gttcaccgac gcgctggccg gcggcgccgc ggcgctcgcc ccgtgggaga tcgccggcat
     6961 cgggcggctc tgggaacgcg tcgacatcga gcgcgccgcc gcctgctacc gcgacgcgat
     7021 cgccgccggc ctcgacggcc gcgatggcca ccgcgtgcgc ctgcgcctgg ccctctggga
     7081 gaagcgccag gcccggtggg acgccgcctg cacgctgtgg caggccgccg cccgcgcggc
     7141 cgccttcgac ccccagccgt gggaggaact ggcgaagttc cacgagcacc gccggcgcga
     7201 tttcgccgcc gcgcgcgcgg tggtgctcca ggccctcgac ctcgctcgta acacgggtgc
     7261 cccgccgcgc gtgctggcag ccttcgccta ccgactggcg cgcctcgaac gccggctggg
     7321 ccgcgaaggc cgagccgagc cgggccactc ctttctggac ccggtggcgc cgacatgacg
     7381 gccatggtca ccgacgtcgg ccctgccggg ccaccctcgg gcgacctccg ccgggactac
     7441 cgaaccggga cgatcaccgt cgtgctggcg gcctcggcgt ctacgtggtc gtcgccgaag
     7501 tgatcaaggc gacccgcctg ggcctcggct caaggtggcg acgatcgccg cgttcgcgat
     7561 gtgcgaatcg gtcgcggccc tcgggctggt gctgtccctg atgtggggca ggcaggtcga
     7621 cgtcctgctg ctcgtcgcgt cgcgctcgtg ggtcaggtag cccggttccc gcggcgggag
     7681 cgctggcagg agtggcgcgc gcgggagcgc ggtgatcgac cgcgctcagt ccgcagggaa
     7741 cgtgacgacg tggagcacgt gcgcctcggg gcgcacgcgc tggccagtgg cgaaggccgc
     7801 cgacgagggc tgcgaggagt gcacgcggtg gccggaggcc aggccgaggc agtagacgtt
     7861 ctccgatccc cggaagtacc gccggaggat gacggcctcg ccctcgtcgg ccgggacg
//
LOCUS       DATIAZ010000153         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1223747, whole genome shotgun sequence.
ACCESSION   DATIAZ010000153 DATIAZ010000000
VERSION     DATIAZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223747"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VLF19_05350"
     CDS             <1..305
                     /locus_tag="VLF19_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92711.1"
                     /translation="QKADDVLGRMAREAGAGTVVVSDDRAVADAARRAHCTVVGGEAF
                     LAALADATSGAVQDAGDDDEDDEDAAASDRPRRGNPRRPSRDDRAALRALRRLRSS"
     gene            424..948
                     /locus_tag="VLF19_05355"
     CDS             424..948
                     /locus_tag="VLF19_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HSE92712.1"
                     /translation="MRSGTRLATTLAALLVLAAGAAGDRLAPARPAFGQPHASQPAGI
                     QGAVVRVVDGDTIHVRVGGRVEKVRYIGVDTPEIRHPQRGEEPGGREAMALNRRLVEG
                     KTVRLELDVRSRDRHGRLLAYVWVGETMINAELVRQGYAQVMTVPPNVRHQDLFLRLQ
                     RAARHEGRGLWKRA"
     gene            1082..>2003
                     /locus_tag="VLF19_05360"
     CDS             1082..>2003
                     /locus_tag="VLF19_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid ferredoxin oxidoreductase"
                     /protein_id="HSE92713.1"
                     /translation="MAIAHEFSLDAKYRKEQGRIFLSGIQALVRLPLDQHRADRRHGL
                     NTATLISGYRGSPLGGLDLTLERNRPLLREHQVVFLSGVNEDLGATAVYGSQLANLFP
                     RAKYDGVLGMWYGKGPGVDRTGDIFKHANYAGVGRHGGVLALGGDDPLSKSSTLPTHS
                     EVAFYDALFPVLFPGSVQEVLDLGRLGFELSRYSGLWVGFKIVTNVADEIGTAEVSQD
                     RLVVRRPDLTFEGRPWQAQQAPMLMPPFGLETERVIQYGRLEAARAFAAANGINRITV
                     PTPGAWLGIAAAGKTYYDLREALRDLGLDDE"
BASE COUNT          276 a          743 c          725 g          259 t
ORIGIN      
        1 cccagaaggc cgacgacgtg ctgggacgca tggcccgcga ggcgggcgcc ggcaccgtcg
       61 tcgtcagcga cgaccgtgcg gtggccgacg ccgcgcgccg agcgcactgc acggtggtcg
      121 gcggcgaggc ctttctcgcc gccctcgcgg acgcgacgag cggggccgtg caggacgccg
      181 gggacgacga cgaggacgac gaggacgcgg cggcgagcga caggccccgg cgcggcaacc
      241 cgcgtcgtcc gtcccgggac gaccgcgccg cccttcgcgc gctgcgccgt ctccgcagct
      301 cctgatcgtg gcgcgcggcc ggagcctcgt cggtctggcg cggctggcgc cggccccggc
      361 atgtgccctg acctgcgtat caggatgttg acactgatgc gcgaggcgtg acagcgtggg
      421 cgcgtgcggt cgggcacgcg cctcgcgacg actctcgccg cgctcctcgt cctcgccgcc
      481 ggcgccgcgg gcgacaggct tgcccccgcg cgaccggcgt tcgggcagcc ccacgcgtcg
      541 cagccggccg gcatccaggg cgcggtggtc cgcgtcgtgg acggcgacac catccacgtc
      601 cgcgtcggcg gccgggtcga gaaggtccgc tacatcggcg tggacacgcc cgagatccgg
      661 cacccgcaga ggggcgagga gcccgggggg cgcgaggcca tggcgctcaa tcgccggctg
      721 gtggaaggca agaccgtgcg gctcgagctg gacgtccgat cacgcgaccg ccacgggcgg
      781 ctcctggcct acgtgtgggt gggcgagacg atgatcaacg ccgagctcgt ccgccagggc
      841 tacgcgcagg tcatgacggt cccgcccaac gtgcggcacc aggacctgtt tctcagactc
      901 cagcgggccg cccggcacga gggcaggggg ctctggaagc gggcctgacg accggcgtca
      961 cctcgcgccc gatcggctcg atcgccacga ggtcgtccat gtcgtcgctc ccgctcgccg
     1021 agtttgcgcc ggtggggacg cgcccgtatc atacgggcag accgcgccac ccgggggggc
     1081 catggcgatc gcgcacgagt tcagcctcga cgccaagtac cggaaagagc agggcaggat
     1141 cttcctctcg gggatccagg ctctcgtcag gctgccgctc gaccagcacc gcgccgaccg
     1201 gcgccacggc ctcaacaccg ccacgctgat ctccggctat cgtggctcgc cgctcggcgg
     1261 cctcgacctg acgctcgagc gcaaccgccc gctcctgcgc gagcaccagg tcgtcttcct
     1321 ctccggggtc aacgaggatc tcggcgcgac ggcggtgtac ggcagccagc tcgccaacct
     1381 gttcccgcgc gcgaagtacg acggcgtgct cggcatgtgg tacggcaagg ggcccggcgt
     1441 ggaccgcacg ggcgacatct tcaagcacgc caactacgcg ggcgtcgggc gtcacggcgg
     1501 cgtgctcgcg ctcggtggcg acgacccgct ctcgaagtcc tccacgctgc cgacgcactc
     1561 ggaggtggcg ttctacgacg cgctcttccc ggtgctcttc cccggcagcg tccaggaggt
     1621 gctcgacctc ggccgactcg gcttcgagct cagccggtac tcggggctct gggtcgggtt
     1681 caagatcgtg accaacgtcg ccgacgagat cggcaccgcc gaggtgagcc aggaccggct
     1741 ggtcgtgcgc cgacccgacc tcaccttcga gggccgcccc tggcaggcgc agcaggcgcc
     1801 catgctgatg ccgcccttcg gcctggagac cgagcgggta atccagtacg gccggctcga
     1861 agccgccagg gccttcgccg cggccaacgg gatcaaccgc atcaccgtgc cgacgcccgg
     1921 ggcgtggctc ggcatcgccg cggccggcaa gacgtactac gacctgcgcg aggcgctgcg
     1981 cgacctcggc ctcgacgacg agg
//
LOCUS       DATIAZ010000154         9234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_171653, whole genome shotgun sequence.
ACCESSION   DATIAZ010000154 DATIAZ010000000
VERSION     DATIAZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171653"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..695
                     /locus_tag="VLF19_05365"
     CDS             51..695
                     /locus_tag="VLF19_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE92714.1"
                     /translation="MKDLDPAVAARIPPGQTLTTKWPVLTYGLTPRVDLKRWSFRCHG
                     LVERETTWTWEEFLRLPRVTVRSDIHCVTRWSKLDNEWEGVPARVIIDHVRPLPQARW
                     VLQHADPDYTTNTALDDLRDDDVLLALRHNGRDLEPDHGGPLRLVLPKLYFWKSSKWL
                     RAFEFLDVNPPGFWEQNGYHMHADPWTEERYSDQETRAMQTMRAEAARRLRDRA"
     gene            892..1791
                     /locus_tag="VLF19_05370"
     CDS             892..1791
                     /locus_tag="VLF19_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSE92715.1"
                     /translation="MAHVHRETAAGRSRGRLLAVLALTGAFAVVEVAGAVWTGSLALA
                     ADAGHMLTDVAGLALALFAVWIATRPPTPAKTFGYYRVEILASMVNALVLLAVAGFIL
                     VEAYVRTFAPREVLAGPMVVVAAAGLAVNLGGAWILHAGAGESLNIRAAYFEVLADAL
                     ASLGVLLAAAAIAVTGWNVIDPLTSAAIAVFIVPRTWRLLTQAVNVLLEGTPAHLALA
                     DVEAAMTAVPGVRYVHDLHVWTLTSGREAMSAHVVVDDPHDSERLLEALHAVLHARFG
                     IDHTTIQIDKAPALVLRLKSREE"
     gene            1865..3415
                     /locus_tag="VLF19_05375"
     CDS             1865..3415
                     /locus_tag="VLF19_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HSE92716.1"
                     /translation="MATTYALTERAIVLRAEDDVAIAKKELAAGDVLEDSGGRVEVRQ
                     DVRPGHKVARRAVRAGQPIRRYGQVIGFATRDIAAGDHVHSHNLDIGELSADRYEIGV
                     DVRPVEHHPPSQMRYFDGFAREDGRIGTRNYVAIVSAVNCSASVSQFVKDRFRDVKVD
                     YPNVDGVIAVTHKSGCGTKLFGEDHMALQRVLAGYAKHPNVAAYILIGLGCEVNQAAV
                     MIDRQKMSLAGRPERTPFLVNIQEAGGIRKTVERAAAEVAKLLPAANAARRTKQPVSE
                     LVLATNCGGSDGNSGITANPALGWAVDELVRYGGTGVLAETPEIYGAEHLLIRRAIDE
                     GVAKKLIDRYKWWEWYCRGIEAMDNNPAPGNKAGGITTVFEKSLGGVTKGGTTPMMDV
                     YLYGEPITTRGFVFMDTPGHDPVSITGLVAGGCNVICFTTGRGSVFGCKPVPSLKLAT
                     NSLMYRHMEEDMDIDCGVILEGTPLATVGRQIFEEVIAVASGKRTKSELSGVGEEEFA
                     PWIIGPVM"
     gene            complement(3396..4064)
                     /locus_tag="VLF19_05380"
     CDS             complement(3396..4064)
                     /locus_tag="VLF19_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSE92717.1"
                     /translation="MTRLPPVAAVVFDMDGVLVDSEPMNFAAMRRMLGDHGIDYTEAD
                     DRRFRGRRNLDLYAWLRASRTGVPDDDVLDRGFTETLAAMIRVACAPMAGVPAVLLAL
                     RERGYRLALASSSAPAIIAETLRALRTGELFAAVVSGVDVARGKPAPDIFVETARRLG
                     VAPQRCLVVEDSRNGMLAARAAGMACVVVPCAATRDEDFREAHARLSSLPALLALLPT
                     SPAR"
     gene            complement(4066..4872)
                     /locus_tag="VLF19_05385"
     CDS             complement(4066..4872)
                     /locus_tag="VLF19_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSE92718.1"
                     /translation="MSVRGLALHTWTLDTTPLPAALAVVRAAGWGAVELRRIDFERAR
                     EAGRTPEQVMAEVRASGLAVACVGARFGWMWEDRVEERTTLLGALDDAARWAAALGCA
                     TVMSPVDRGRGDTRRAAASVREAGDVVAAHGVTLAVEPNSQCEQLNTIERVREVLALA
                     GHPRCGLLVDTYHVQRAGDWRALEDLPRGEIAYVQYSDVPRETRPGFALDRLPPGRGV
                     VPFREAFALVAASGYSGWLSYEAPNEAAWKRPPDEVAREALEASRPLLPQ"
     gene            complement(4869..5156)
                     /locus_tag="VLF19_05390"
     CDS             complement(4869..5156)
                     /locus_tag="VLF19_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HSE92719.1"
                     /translation="MAEQVVPFGDVEETTGTAIAPPWMTILHNCDCHTFEEVVKQLQK
                     AIACSESKGWEIAWQVHNSGKAVVKIGPETECVRVGNVLAAIGLVVTVVQS"
     gene            complement(5273..6499)
                     /locus_tag="VLF19_05395"
     CDS             complement(5273..6499)
                     /locus_tag="VLF19_05395"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92720.1"
                     /translation="MSRPPLDGLRVLAVSQFGAGPFGTQVLADLGAEVIKIEDPAQGG
                     DVSRYVPPWQIEGDSLYFQAFNRGKKSITLDLRHPEGRAVFHDLVRVSHAVYNNVRGD
                     LPRKLGLTYETLGAVNPAVVCCSLSGFGSTGPRAAEPGYDYLIQGHAGYMAITGEPDG
                     PPGKCGVSVIDFAGGYASMLALMVGLWDAQRSGVGRDLDVSLLDTAVSMLSYFAAWTL
                     NRDWQPQRVADSGHQTLVPSQNFRTADGWIVVFCAKEKFWQALAEALDLGPLACDPRF
                     ASFPDRLQHKDALLAVLQPRFRERPTAEWLARLRGRVPCAPVNTVRQALEDEQVRARE
                     MIVEVDHPDYGVIREVASPIKTAGAVRRPAPAPRLGQHTEAVLRDVLRYPPARISALR
                     ASGALGASRLPSPPAT"
     gene            complement(6496..7092)
                     /locus_tag="VLF19_05400"
     CDS             complement(6496..7092)
                     /locus_tag="VLF19_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSE92721.1"
                     /translation="MGYGRWLEEFTVGEVIEHWPGRTITETDNTWFALLTNNQHPLHT
                     DAHYAETSTQHGQRVVLGPLVFAVGIGLTVGDVSGRAIANLEIERIEHHAPTFNGDTL
                     YSESTVLDVRRSRQGDRGVVAVETRVSNQRGERVMTFRRKVLVPTRNHPTLGDRTPRP
                     ASPAATPPRRPSGVAKDGDSVDAGDRAPAATTKRRRRR"
     gene            complement(7092..8783)
                     /locus_tag="VLF19_05405"
     CDS             complement(7092..8783)
                     /locus_tag="VLF19_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSE92722.1"
                     /translation="MVARRVRLASGLVLFAYVSTHLSNHALGLVSLDAIEIGRGWFLA
                     LWRSLPGTLALYGALATHFGLALYSLARRRHFRMPAWEATQILLGLSIPPLLLGHVVG
                     TRVAHEVFGADDPYARVVLTLGVLRPDRGAWQAVALVVAWSHGCAGLHYWLRLRRSYA
                     RLAPVLLAAAVLLPTLALLGFVNAAREVAVLAREPGWADAMLRALGAPDAGARATLEL
                     VQRAAEWTFGASLAAVALVHAVRLARARRWTGVVYPDGRQVRIPPGATVLEASRLGGI
                     PHASVCGGRGRCSTCRIRVTHGLEHAPPPSEDERHVLARVGAPPNVRLACQLRPTRDV
                     AVVPLLVPSATPRDARPRPAAHAGREQEVAVLFADLRGFTALAERKLPYDVVFILNRY
                     FEAVGTAIQRAGGVANQFTGDGVMALFGVEGGPEDACRRALVAAGEVIASLDALNRHL
                     AAELPAPLRIGIGVHTGPAVVGQMGHGPALYLTAVGDTVNTAARLEELTKTYRCELVI
                     SEPVAGRAGVDVSEYPRHELMLRNRTTPLAVRVVDDARRLAAALRRATPGPVRET"
     gene            complement(8881..>9234)
                     /locus_tag="VLF19_05410"
     CDS             complement(8881..>9234)
                     /locus_tag="VLF19_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92723.1"
                     /translation="ALRYSQQRSAFGQAICHHQAIQLKLADMATAITAARLLAYDAAE
                     RLEAGDDLATGLARLQACETAGMVTLEAMRIHGGYGYTTEFLVERYYRDAPRLALGLG
                     GLEAERAALARRLVG"
BASE COUNT         1344 a         3314 c         3369 g         1207 t
ORIGIN      
        1 tggcgcgcat cctcttcgag ctctggaggc aacgacgcat gcaaggccgg atgaaagacc
       61 tcgaccccgc ggtcgccgcg cgcatcccgc ccgggcagac gctcaccacc aaatggcccg
      121 tcctgaccta cggcctcacg ccccgcgtcg acctcaagcg gtggtcgttc cggtgccacg
      181 ggctcgtgga gcgcgagacg acgtggacct gggaggagtt cctgcgcctg ccgcgggtga
      241 ccgtgcgctc cgacatccac tgcgtgaccc gctggtcgaa gctcgacaac gagtgggagg
      301 gcgttccggc ccgcgtcatc atcgatcacg tgcggccact gccgcaggcg cggtgggtgc
      361 tccagcacgc ggaccccgac tacacgacca acactgcgct cgacgacctg cgcgacgacg
      421 atgtgctgct ggcgctcagg cacaacggcc gagacctcga gcccgatcac ggcgggcccc
      481 tgcggctcgt ccttcccaag ctctacttct ggaagtcgag caagtggctt cgcgccttcg
      541 agttcctcga cgtcaacccg cccggcttct gggagcagaa cggctaccac atgcatgccg
      601 atccgtggac ggaggagcgc tactccgacc aggagacgcg ggcgatgcag acgatgcgcg
      661 ccgaggccgc gcgcaggctc cgcgaccgcg cctaggcgct tcgcccctgg cccctcgcgc
      721 agccgggctt ccgtgtagcg gggattcgcc gcgcggacca gggccggcgc cgacgacgga
      781 cccactcggt gtagtggaaa cggtggcctc cgagatcgac ggagccgtcg acgaccgcgc
      841 ccggcgcccg cattcgtgcg tcatctcacc aggacctgct acgatcctcc gatggctcac
      901 gtccatcgcg agaccgccgc cggacgctcg cgcggacggc tgctcgccgt gctcgcgctg
      961 acgggcgcgt tcgccgtcgt cgaggtcgcc ggtgccgtgt ggacgggaag cctcgccctg
     1021 gccgcggatg ccggccacat gctcaccgac gtggccggcc tcgcgctcgc gctgttcgcc
     1081 gtgtggattg ccacccggcc ccccacgccg gccaagacct tcggctacta ccgcgtggag
     1141 atcctggcct cgatggtcaa cgccctcgtg ctgctcgccg tcgccggctt catcctcgtc
     1201 gaggcctacg tgcgcacctt cgcgccgcgc gaggtgctgg ccgggccgat ggtcgtcgtc
     1261 gccgcggcgg ggctcgccgt caacctcggt ggcgcctgga tcctgcacgc cggtgccggc
     1321 gagagcctca acatccgcgc ggcctacttc gaggtcctcg ccgatgcgct cgcctcgctg
     1381 ggcgtgctcc tcgcggcggc ggccatcgcc gtcaccggct ggaacgtgat cgaccccctc
     1441 accagcgcgg cgatcgccgt cttcatcgtg ccgcgcacgt ggcggctgct cacccaggcc
     1501 gtcaacgtgc tgctcgaggg tacgccggcg cacctggcgc tcgccgacgt cgaggcggcc
     1561 atgacggccg ttcccggcgt gcgctacgtc cacgacctgc acgtgtggac gctgacgtcg
     1621 gggcgcgagg cgatgagcgc tcacgtcgtg gtggatgatc cccacgacag cgagcgcctg
     1681 ctcgaggcgc tgcacgccgt gctgcacgcc cggttcggca tcgaccacac cacgatccag
     1741 atcgacaagg cgcccgcgct ggtcctccgc ctcaagtcgc gcgaggagta gcggcgcccc
     1801 gccgggttgt atagtgagac ggccgggcct gccccgggca gtcaacccac gctcgaggtg
     1861 acacatggcg acaacctacg cgctgaccga gcgggcgatc gtgctccgcg ccgaggacga
     1921 cgtcgcgatc gcgaagaagg agctcgcggc gggcgacgtg ctggaggact ccggtggacg
     1981 cgtcgaggtg cgccaggacg tgaggcctgg ccacaaggtc gcgcggcgcg ccgtgcgggc
     2041 gggccagccc atccggcgct acggccaggt gatcgggttc gccacccgcg acatcgccgc
     2101 cggcgatcac gtccacagcc acaacctgga catcggcgag ctgtcggccg accggtacga
     2161 gatcggcgtg gacgtccggc cggtggagca ccacccgcct tcgcagatgc gctacttcga
     2221 cggcttcgca cgggaggacg gacggatcgg cacgcgcaac tacgtggcga tcgtctccgc
     2281 cgtgaactgc tcggcgagcg tcagccagtt cgtgaaggac aggttccgtg acgtgaaggt
     2341 ggactacccg aacgtcgacg gcgtcatcgc cgtcacccac aagtccggct gcggcaccaa
     2401 gctgttcggc gaggaccaca tggcgctcca gcgcgtgctc gccggctacg ccaagcaccc
     2461 gaacgtggcg gcgtacatcc tgatcgggct cggctgcgag gtgaaccagg cggcggtcat
     2521 gatcgaccgc cagaagatgt cgctggccgg ccgccccgag cgcacgccgt tcctggtcaa
     2581 catccaggag gcgggcggta tccggaagac ggtcgagcgg gccgccgcgg aggtggcgaa
     2641 gctcctgccg gcggcgaacg ccgcgcggcg cacgaagcaa ccggtgtcgg agctggtcct
     2701 cgcgacgaac tgcggcggtt ccgacggcaa ctcggggatc accgccaacc ccgccctcgg
     2761 ctgggcggtg gatgagctgg tccgctacgg gggcaccggc gtgctcgcgg agacgccgga
     2821 gatctacggc gccgagcacc tgctcatccg gcgggcgatc gacgagggcg tggccaagaa
     2881 gctcatcgac cgctacaagt ggtgggagtg gtactgccgg ggcatcgagg cgatggacaa
     2941 caaccccgca cccggcaaca aggccggcgg catcaccacg gtcttcgaga agtctctggg
     3001 cggcgtgacc aaggggggca cgacgccgat gatggacgtg tacctctatg gcgagcccat
     3061 caccacgcgc ggcttcgtgt tcatggacac gccgggccac gacccggtgt cgatcacggg
     3121 gctcgtcgcc ggcggctgca acgtcatctg cttcaccacc ggccgcggct cggtcttcgg
     3181 ctgcaagccc gtgccctctc tcaagctggc cacgaactcg ctgatgtatc gccacatgga
     3241 agaggacatg gacatcgact gcggcgtgat tctcgagggc acgccgctgg ccacggtcgg
     3301 ccgtcagatc ttcgaggagg tcatcgcggt ggcctccggc aagcggacca agagcgagct
     3361 cagcggcgtc ggcgaggagg agttcgcgcc ctggatcatc gggccggtga tgtaggcagc
     3421 agcgccagca gcgccggcag gctcgacaga cgcgcgtgcg cctcgcggaa gtcctcgtcg
     3481 cgcgtggccg cgcagggcac gacgacacag gccatccctg cggcccgggc ggccagcatg
     3541 ccgttgcgcg agtcctcgac gacgaggcag cgttgggggg ccacgccgag ccgccgcgcg
     3601 gtctcgacga agatgtccgg cgccggcttg ccccgggcga cgtccactcc cgacacgacg
     3661 gccgcgaaga gctcgccggt gcggagcgcc cgcagcgtct cggcgatgat ggcgggcgcg
     3721 gacgacgagg ccagggccag ccggtagccg cgctcgcgga gcgccaggag gaccgcgggg
     3781 acgccggcca tgggcgcgca cgcgacgcgg atcatcgccg ccagcgtctc ggtgaacccg
     3841 cggtcgagga cgtcgtcgtc cggcacgccg gtgcggctgg cgcggagcca ggcgtagaga
     3901 tcgaggttgc ggcgaccccg gaaccgccgg tcgtccgcct ccgtgtagtc gatgccgtga
     3961 tccccgagca tgcgtcgcat cgccgcgaag ttcatcggct cggagtcgac gagcacgccg
     4021 tccatgtcga agacgacggc ggccaccggc ggcaggcggg tcacgtcact ggggcaacag
     4081 cggtcgtgac gcctccagcg cctcgcgcgc gacctcgtcc ggcggacgct tccacgcggc
     4141 ctcgttcggc gcctcgtagg agagccagcc ggaataaccg ctggccgcga cgagcgcgaa
     4201 cgcctcccgg aacggcacca cgccgcggcc gggcggcagc cggtcgagcg cgaaccccgg
     4261 ccgcgtctcg cgcggcacgt cactgtactg cacgtaggcg atctcgccgc gcgggagatc
     4321 ctccagggcg cgccagtcgc cggcgcgctg cacgtggtac gtgtcgacga ggagaccgca
     4381 gcgggggtgg ccggcgaggg cgagcacctc gcgcacacgc tcgatcgtgt tgagctgctc
     4441 gcactgcgag ttcggctcga cggccagcgt cacgccgtgc gcggccacga cgtcgccggc
     4501 ctcgcggacg ctcgcggcgg cgcgccgggt gtcgccgcgc ccacggtcga ccgggctcat
     4561 caccgtcgcg cagccgagcg ccgccgccca cctggccgcg tcgtcgagcg cgccgaggag
     4621 cgtggtgcgc tcctcgaccc tgtcctccca catccagccg aatcgtgcgc cgacgcaagc
     4681 cacggcgagc ccgctggcgc gcacttcggc catcacctgc tcgggcgtgc gaccggcctc
     4741 ccgtgcgcgc tcgaagtcga tccgccggag ctcgaccgcg ccccagccgg ccgcacgcac
     4801 gacggcgagt gccgcgggca gcggcgtggt gtcgagggtc caggtgtgga gcgcgagccc
     4861 gcgcacgctc acgactgcac cacggtgacg acgaggccga tggccgcgag cacgttgccc
     4921 acgcgcacgc actcggtctc gggcccgatc ttcaccaccg ccttgccgct gttgtgcacc
     4981 tgccaggcga tctcccagcc cttcgactcg ctgcaggcga tggccttctg gagctgtttc
     5041 accacttcct cgaaggtgtg acagtcgcag ttgtggagga tggtcatcca gggcggcgca
     5101 atcgccgtcc ccgtcgtctc ctcgacgtcg ccgaacggaa ccacctgctc ggccatggcc
     5161 ctgacccctc tcgggccccc cattatccca cccgcctcaa ggcgcggcgg agagccgccc
     5221 gtcccatggg cgtcgcgcga ccgcggaccg cagtgtccgc atgggtggca cctcaggtcg
     5281 cgggcgggga cggcaacctc gacgcgccca gtgcgcccga tgcgcgcagg gccgagatcc
     5341 tggcgggagg atagcgcaag acgtcgcgga gcacggcctc ggtgtgctgg cccagccgcg
     5401 gcgccggcgc cgggcggcgc acggcgccgg ccgtcttgat cggcgaggcc acctcgcgga
     5461 tcacgccgta gtccgggtgg tccacctcga cgatcatctc ccgggcgcgt acctgctcat
     5521 cctcgagcgc ctggcgcacg gtgttcaccg gcgcgcaggg cacgcgcccg cgcagtcgcg
     5581 ccagccactc ggcggtgggg cgctcgcgaa agcgtggctg gaggacggcg agcagcgcgt
     5641 ccttgtgctg caggcggtcg gggaagctcg cgaagcgcgg atcgcacgcg agggggccga
     5701 ggtccagggc ctccgcgagc gcctgccaga acttctcttt ggcgcagaac acgacgatcc
     5761 agccgtccgc cgtccggaag ttctgcgacg gcaccagcgt ctggtggccg gagtcggcga
     5821 cacgctgggg ctgccagtcg cggttgagcg tccacgccgc gaagtacgag agcatgctca
     5881 cggccgtgtc gaggagcgag acgtcgagat cgcggccgac gcccgagcgc tgcgcgtccc
     5941 agaggccgac catgagggcg agcatcgagg cgtagccgcc cgcgaagtcg atgacggaga
     6001 cgccgcactt gcccggcggg ccgtcgggct cgccggtgat ggccatgtag ccggcgtggc
     6061 cctggatgag atagtcgtag ccgggctcgg cggcgcgcgg cccggtcgag ccgaagccgg
     6121 agagcgagca gcagacgacg gcagggttca cggcaccgag cgtctcgtag gtgagcccga
     6181 gctttcgagg caggtcccct cgcacgttgt tgtagaccgc gtgcgagacg cggaccagat
     6241 cgtggaagac cgcgcggccc tcgggatggc gcaggtcgag cgtgatggac ttcttcccgc
     6301 gattgaaggc ctggaagtag agcgagtccc cctcgatctg ccagggcggg acgtagcggc
     6361 tcacgtcgcc accctgggcg ggatcctcga tcttgatcac ttcggcgccc aggtcggcga
     6421 gcacctgggt cccgaacggc ccggcgccga actgcgagac ggcgagcacg cggagaccat
     6481 cgagcggcgg ccggctcatc ggcgccgacg tctcttcgtc gttgccgccg gagcgcgatc
     6541 tccggcatcg acggagtcgc cgtccttcgc gacaccgctc ggccgccgcg gtggcgtggc
     6601 ggccggcgac gccgggcgtg gcgtccgatc tcccagggtc gggtggttgc gcgtgggcac
     6661 gagcaccttg cggcggaagg tcatcacgcg ctcgccgcgc tggttgctga cgcgggtctc
     6721 cactgcgacc acgccgcggt ccccctgccg cgacctgcgc acgtcgagca ccgtggactc
     6781 ggaatagagc gtgtcgccgt tgaaggtcgg ggcgtggtgc tcgatccgct cgatctcgag
     6841 gtttgcgatg gccctgccgg agacgtcgcc gacggtgagg ccgatgccga ccgcgaacac
     6901 gagcgggccc agcacgaccc gctggccgtg ctgcgtggac gtctcggcgt aatgcgcgtc
     6961 ggtgtgcagc gggtgctgat tgttcgtgag cagcgcgaac caggtgttgt cggtctcggt
     7021 gatcgtgcgg cccggccagt gctcgatgac ctcgcccacc gtgaactcct cgagccagcg
     7081 cccgtatccc atcaggtctc ccggaccggg ccgggggtcg cgcggcggag cgcggcggcg
     7141 agccggcgcg cgtcgtccac gacgcggacg gcgagggggg tagtcctgtt acgcagcatg
     7201 agctcgtggc gcggatactc ggagacgtcc acgcccgcgc gaccagccac cggctcggag
     7261 atcaccagct cgcaccggta cgtcttcgtc agctcctcca gccgggcggc ggtgttgact
     7321 gtatcaccga cggccgtgag gtacagcgcc ggcccgtggc ccatctgccc cacgacggcg
     7381 ggtcccgtgt gcacgccgat gccgatgcgg agcggcgccg gtagctcggc cgcaaggtgg
     7441 cggttgaggg catccaggct cgcgatgacc tcgccggcgg cgacgagcgc ccggcggcag
     7501 gcgtcctcgg gcccgccctc caccccgaac agcgccatga cgccgtcgcc cgtgaactgg
     7561 ttcgccacgc cgcccgcgcg ctggatggcg gtgcccacgg cctcgaagta acggttgagg
     7621 atgaacacga cgtcgtaggg tagcttacgc tccgccagcg cggtgaaacc ccggaggtcg
     7681 gcgaagagca cggcgacttc ctgctcccgt ccggcgtggg ccgccggacg cggccgcgcg
     7741 tcgcgcggcg tcgccgacgg gacgagcagc ggcaccacgg cgacgtcgcg tgtgggccgg
     7801 agctggcagg caagccgcac gttcggcgga gcgccgacgc gagccaggac gtgccgctcg
     7861 tcctcgctcg gcggcggcgc gtgctcgaga ccgtgcgtga cgcggatgcg gcacgtcgag
     7921 caccggccgc gcccgccgca gacagaagcg tgcgggatcc cgccgaggcg gctcgcctcg
     7981 aggaccgtcg ccccgggcgg gatgcgcacc tgacgtccgt ccggatagac cacgcccgtc
     8041 caccgccgag cgcgggcgag ccggacggcg tgcaccaggg cgaccgcggc gagcgacgcg
     8101 ccgaacgtcc actcggccgc gcgctgcacg agctcgaggg tggcgcgcgc cccggcgtcc
     8161 ggcgccccca gcgcgcggag catggcgtcg gcccagcccg gctcgcgggc cagcacggcc
     8221 acctcgcggg cagcgttcac gaagccgagg agcgccagcg tcggcagcag cacggcggcg
     8281 gccagcagga cgggcgccaa gcgcgcgtag ctgcggcgga gccggagcca gtagtggagc
     8341 ccggcgcagc cgtgggacca tgcgacgacc agtgccacgg cctgccaggc gccgcggtcg
     8401 gggcgcaaca cgccgagcgt gagcaccacg cgcgcgtacg ggtcgtcggc gccgaacacc
     8461 tcgtgggcga cgcgcgtgcc gacgacgtgt ccaaggagca gcggcgggat cgacagcccg
     8521 agcaggatct gcgtggcctc ccaggcgggc atgcggaagt gacggcggcg cgcgagcgag
     8581 tacagcgcca ggccgaagtg ggtggccagc gccccgtaga gggcgagcgt gccgggcagg
     8641 ctccgccaca gcgcgaggaa ccacccgcgg ccgatctcga tcgcgtcgag cgacacgagg
     8701 cccagcgcgt gattcgagag gtgcgtggag acgtaagcga agaggacgag cccggaagcc
     8761 agccggaccc gacgggcgac cacggcctag ccgcctcgcg ggtgatcgag gcgcgccaga
     8821 gccgtgccgg ggcgggccgc gccaccctgg gggccagggg gccatgtcgg ggctcccacg
     8881 tcaaccgacc aggcgacggg ccagcgccgc gcgctcggcc tcgaggcccc ccagaccgag
     8941 cgccagccgc ggcgcgtcgc ggtagtaacg ctcgaccagg aactcggtcg tgtagccgta
     9001 gccaccgtgg atgcgcatcg cctccagggt caccatgccc gccgtctcgc aggcctgcag
     9061 gcgcgccagg cccgtggcga ggtcgtcgcc ggcctcgagc cgctcggcgg cgtcgtaggc
     9121 cagcagccgc gcggcggtga tggcggtggc catgtcggcg agcttgagct ggatcgcctg
     9181 gtggtggcag atcgcctgcc cgaaggcgga gcgctgctgc gagtagcgga gggc
//
LOCUS       DATIAZ010000155         5587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1224401, whole genome shotgun sequence.
ACCESSION   DATIAZ010000155 DATIAZ010000000
VERSION     DATIAZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224401"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /locus_tag="VLF19_05415"
     CDS             complement(<1..1764)
                     /locus_tag="VLF19_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE92724.1"
                     /translation="MTTDTSEIIWRPTPDAAARARLSSLMRAQGIATLAELQRRSIAD
                     PEWYWTAVVRDLGIRWLTPYTRVLDASRGPAWPAWFPGGRLNLADTCVDRHVDAGRGG
                     KPALVWEGDDGGTRTLTYAELAAEVGRLANALARLGVGEGDRVGIFLPMSPEAAIATL
                     AVVRLGAIYTPCFSGFGAQAVASRLQDSEAKMLITADGFHRRGQVVRMKETADEAVAM
                     SPTVERVIVYRRLGRDVPWRAGRDRWWHEETARQSDQRPVNPVAADHPCLIIYTSGTT
                     GRPKGAVLTHGGFLVKCAHDFAYCHDVGESDRLFWLTDLGWLMGPMLVTAALAHGATG
                     VLFEGVPDYPKPDRLWALVARHRITVMGLSPTAVRALMPHGADHPRAHDLSSLRILGS
                     TGEPWNPEPYRWLFDHAGGGRLPIINYTGGTEISGGILGCFPCAPIKPCSFAGPIPGM
                     AADVFGDDGRPVRGQVGELVVTRPWPGMTAGFWRDPARYEETYWSRWPDVWVHGDWAV
                     VDDDGFWFLQGRSDDTLKIAGKRLGPAEVESILVGHPAVAESGVIGVPHPVKGEAVVC
                     FVVLRPGQTPSEPLRVELSDRV"
     gene            complement(1900..2949)
                     /locus_tag="VLF19_05420"
     CDS             complement(1900..2949)
                     /locus_tag="VLF19_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92725.1"
                     /translation="MGKGPLARGGLLLLMLSLVFVAPVTAGLRLPPGFIARVYVTGQG
                     FGSAAGIAGLPSSSTMGLDETGALYLARTGRRYVTGGEVEDLWQLYRIPAGGGRLTPD
                     NERTFLHGPPLPNPQVALVRSGRDLFVTTYDRDRKVGVLYSVRAGRAEWFAGGTPARG
                     QAPLLQQPEGAAADRAGNLYVADREQGVIVKLDPSGRVLEPRWLVVTRPRTLAMDHAD
                     HLWVGSDGGAQAPWQQGPGMILRVDPDGEPRVMMRGPVAAALAIGPGGQAFVADRHNP
                     SVFILGPDGTRAEFAQFTNNDAPRSIAFAPVTPATRQAGIAGDLFVVTINRGAWPVNE
                     VIRISGPFAELIGAR"
     gene            complement(3123..3284)
                     /locus_tag="VLF19_05425"
     CDS             complement(3123..3284)
                     /locus_tag="VLF19_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92726.1"
                     /translation="MDRGDQDEWDFLTSDPDATCPICGDAIHAGQAVGRINGALVHAK
                     CAPPAPPLR"
     gene            3618..4052
                     /locus_tag="VLF19_05430"
     CDS             3618..4052
                     /locus_tag="VLF19_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92727.1"
                     /translation="MGLAPEFLQSADRLAAAVTCALDREREACAAVAERLGHSHPSWQ
                     GAAASIAAAIRDRGRSPDRQKPEKNGVRRALTLLELSRGRHYCVRCLAASLRSSLRVA
                     QQAMKELSRTEGFDVSRHWCRGCGDNREVVCARLDHAPRRLG"
     gene            complement(4070..4873)
                     /locus_tag="VLF19_05435"
     CDS             complement(4070..4873)
                     /locus_tag="VLF19_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSE92728.1"
                     /translation="MADERPDILALDFDGVLCEGTREYFETSRRTHARVWPERTPAAE
                     ALLPAFRDLRPVIETGWEMPVLLDALVRGLPRKAIGEAWGETRDELLAHDPRGRDRVV
                     AALKTTLDQVRREWIAADLTDWLSRHALYCDREAVRRVVEGPARTVIVTTKEGEFARQ
                     ILEHWGLPVAGIQGKEAGSHKCENLRVLRSAYRAETGRAATLWFVEDRLETLECVRGH
                     ADLDDVRLFLATWGYNTARAREAARRGRGIDPLTLAEFSGPFSGWGQGG"
     gene            5006..>5587
                     /locus_tag="VLF19_05440"
     CDS             5006..>5587
                     /locus_tag="VLF19_05440"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HSE92729.1"
                     /translation="MSQPDAFFVPDGDDRFVATEHGRGPWSLDHLHAGPPSALLARAI
                     ERAGAGDPVVVTRLTVDLLAPVPIGPLEVATSTLRAGRTVRRLEATLAAGGRVLARAS
                     GLVTRTQALDFPPVKPEPCDPPPPPGSGAPFRFPVFHGSAIGYQTAVDARCVRGGFGQ
                     NPTTVWIRARVPLVLGEAPSPLARVMIAADSGNG"
BASE COUNT          804 a         2020 c         2017 g          746 t
ORIGIN      
        1 cacgcggtcg ctgagctcga cgcgcagcgg ctcggacggt gtctggcccg ggcgcagcac
       61 gacgaagcag acgacggcct cgcccttcac cgggtggggc acgccgatga cgccggactc
      121 cgcgacggcc ggatggccga ccaggatcga ctcgacctcc gccgggccca ggcgcttgcc
      181 ggcgatcttc agcgtgtcgt cggagcggcc ctgcaggaac cagaagccgt cgtcgtcgac
      241 caccgcccag tcgccgtgga cccacacgtc gggccagcgc gaccagtagg tctcctcgta
      301 gcgcgcggga tcgcgccaga agccggcggt catgccgggc cagggcctgg tcaccacgag
      361 ctcgcccacc tggccgcgca cgggacggcc gtcgtcgccg aagacgtcgg cggccatgcc
      421 gggaatcggg cccgcgaacg agcacggctt gatcggcgcg cacgggaagc agccgaggat
      481 gcctccggag atttccgtcc caccggtgta gttgatgatc ggcaggcggc cgccgccggc
      541 gtggtcgaag agccagcggt agggctcggg attccacggc tcgccggtcg agccgaggat
      601 gcgcagcgac gacagatcgt gggcgcgcgg atgatcggcg ccgtgaggca tcagcgcgcg
      661 cacggcggtc ggcgagagcc ccatcacggt gatccgatgg cgagcgacga gcgcccacag
      721 ccggtcgggc ttgggataat cgggcacgcc ctcgaacagc acgccggtgg cgccgtgggc
      781 gagcgccgcg gtgacgagca tcggacccat gagccagccg aggtcggtga gccagaagag
      841 ccggtcgctc tcgcccacgt cgtggcagta cgcgaagtcg tgcgcgcact tgacgaggaa
      901 gccgccgtgc gtgaggacag cgcccttcgg gcgtccggtg gtgcccgacg tgtagatgat
      961 gaggcacggg tgatcggcgg cgacggggtt caccggacgc tgatcggact gacgcgccgt
     1021 ctcctcgtgc caccagcgat cgcgtccggc tctccacggg acgtcgcggc cgaggcggcg
     1081 atagacgatc acgcgctcga cggtcggcga catcgccacc gcctcgtcgg ccgtctcctt
     1141 catgcgcacc acctggccgc ggcggtggaa gccgtcggcg gtgatcagca tcttcgcctc
     1201 ggagtcctgg agccgagagg cgacggcctg ggcaccgaag ccggagaagc acggcgtgta
     1261 gatcgcgccg aggcgcacga cggcgagcgt ggcgatggcg gcctcgggcg acatcggcag
     1321 gaagatgccc acgcgatcgc cctcacccac cccgagccgc gcgagcgcgt tggccaggcg
     1381 cccgacctcg gccgccagct cggcgtacgt cagcgtgcgc gttccgccgt cgtcgccctc
     1441 ccacacgagc gcgggcttgc cgcctcgccc ggcgtccacg tgacggtcca cgcaggtgtc
     1501 ggcgaggttc agccggccgc cggggaacca ggcgggccac gccggcccgc gcgaggcgtc
     1561 gagcacgcgc gtgtacggcg tgagccagcg gatgccgaga tcgcggacga ccgcggtcca
     1621 gtaccactcg gggtcggcga tggagcgccg ctggagctcg gccagcgtcg cgataccctg
     1681 tgcacgcatg aggctggaca gccgcgcgcg cgcggcggcg tcgggcgtgg gccgccagat
     1741 gatctcgctc gtgtcggtcg tcatcggtca cctctccgga acaggcagcc ccattgtggc
     1801 gcggcggcgc cagcccgctc aagccgggcc acgtggcccc ggcgaaccgg aagcatttcg
     1861 tcagcgcgct ggccgggctc gagcggccag cgcccgggct cagcgggcgc cgatcagctc
     1921 cgcgaacggc cccgagatcc ggatcacctc gttcaccggc caggcgccac ggttgatcgt
     1981 gacgacgaac aggtcgccag cgatcccggc ctggcgggtc gccggcgtca ccggcgcgaa
     2041 ggcgatgctc cggggcgcgt cgttgttggt gaactgtgcg aactcggcgc gggtgccgtc
     2101 agggccgaga atgaacacgc tcgggttgtg gcggtcggcg acgaaggcct gaccgcccgg
     2161 gccgatggcg agcgcggcgg cgacgggacc acgcatcatc acgcgcggct cgccgtcggg
     2221 atcgacacga aggatcatgc cggggccctg ctgccacggc gcctgcgcgc cgccgtcgct
     2281 gccgacccac aggtgatcgg cgtggtccat ggcgagcgtg cgcgggcgag tcaccacgag
     2341 ccagcgcggc tcgagcacgc gcccggaggg atcgagcttg acgatcacgc cctgctcgcg
     2401 gtcggcgacg tagaggttgc ccgcgcggtc agccgccgca ccttccggct gctggagcag
     2461 cggcgcctgc ccccgcgccg gggtgccgcc ggcgaaccac tcggcgcggc cggcgcggac
     2521 cgagtagagc acgcccacct tccggtcgcg gtcgtacgtg gtcacgaaga ggtcacgccc
     2581 gctccgcacg agggccacct gcgggttcgg cagcggcggg ccgtggagaa aggtcctctc
     2641 gttgtccggc gtgaggcggc caccgccggc ggggatgcgg tagagttgcc agaggtcctc
     2701 gacctccccg ccggtcacgt aacgtcggcc cgtgcgcgcc aggtagaggg cgccggtctc
     2761 gtcgagaccc atcgtggacg acgagggcag gcctgcgatg cctgcggcgg agccgaaccc
     2821 ctgaccggtg acatagaccc gggcgatgaa cccgggcggc aaccgcaggc ccgccgtcac
     2881 tggcgcgacg aacacgagcg acagcatgag cagcagcagc ccaccgcgcg ccaacgggcc
     2941 cttccccatg cgtggtctcc ttcgaaggcc cttcagtgta tccgaggtct gacggagcac
     3001 gcaggcgacc ggcgatagcc gaagacacga gggagcgggg cttcgggccg cgccacggtg
     3061 ccgcatttgg tggcggcgtc cgctggccga ttgggggcat ctcgaagccc ccgtgtcgcg
     3121 ggtcagcgca ggggtggcgc cggcggcgcg cacttcgcgt gcaccagggc gccattgatg
     3181 cgcccgaccg cctgaccagc gtgaatcgcg tccccgcaga tggggcacgt ggcgtccggg
     3241 tcggacgtca ggaagtccca ctcgtcctgg tcgccacggt ccacttgagc tcctgatcaa
     3301 cccggtgcat ctaccgggcg agtgcatcaa ctccatgaaa gatttctcca attctccccc
     3361 agcagctcga cctgcgggga accccctggc gtggtagagg tcgaggagca gcagaaacag
     3421 cacctcccga gacgaccaga gtgaccgtca ccaccatgtt atggcccccc ggcgggcggg
     3481 cggtcccgca atgcatgtgc cgtcgaggct cggcgcgaag gccccatccg attccgcaag
     3541 ccacggccga ttctccatac gggatgtcca cggcgtcgtc gggccaggag caagcgcgcc
     3601 gcctcgttcg agagtggatg gggctggcgc cggagtttct ccagagcgcc gatcgcctgg
     3661 cggccgcggt cacctgcgcg ctggaccgcg aacgggaggc atgcgctgcc gtcgccgagc
     3721 gcctaggaca tagccacccg tcgtggcaag gcgcggccgc cagcatcgcc gccgcgatcc
     3781 gcgatcgcgg ccgcagcccc gaccgccaga agcccgagaa gaatggggtg cggcgcgcct
     3841 tgacactcct cgagctctcc aggggccgcc actactgtgt ccggtgtctc gccgcgagcc
     3901 tgcgctcgag ccttcgagtc gcgcagcagg ccatgaagga gttgtcgcgg accgagggct
     3961 tcgacgtgag caggcactgg tgccgggggt gcggggacaa ccgcgaggtg gtctgcgccc
     4021 ggctggacca tgcgccgcgc cggctaggct gagctcgact actgccggat caccctccct
     4081 gaccccaccc cgagaacggt cccgagaact cggcgagcgt tagtggatcg atgcctctgc
     4141 cgcgccgtgc ggcctctcgg gcgcgggcgg tgttgtagcc ccaggtcgcg aggaagagcc
     4201 gcacgtcgtc gaggtccgcg tggccgcgga cgcactcgag cgtctcaagc cggtcctcca
     4261 cgaaccagag ggtggcggcc cggccggttt cggcccggta cgccgatcgg agcacgcgca
     4321 aattctcgca cttgtggctg cccgcctcct tgccctggat gccggcgacg gggagtcccc
     4381 agtgttcgag gatctggcgc gcaaactcgc cctccttcgt ggtcacgatg accgtcctcg
     4441 ccggcccctc caccacacgc cggacggcct cgcggtcgca gtagagcgcg tgccgcgaga
     4501 gccagtccgt gaggtccgcc gcgatccact cccgacgcac ctgatcgagg gtcgtcttca
     4561 gcgcggcgac gacgcggtct cggccgcggg gatcgtgggc gagcaactcg tcgcgcgtct
     4621 cgccccacgc ctcgccgatg gccttccggg gcaggccgcg cacgagggcg tcgagcaaca
     4681 ccggcatctc ccaaccggtc tcgatcacgg gccggaggtc gcgaaaggcg ggcaacagcg
     4741 cctcggcggc aggcgtgcgc tcgggccaca cccgcgcgtg cgtgcgccgg ctggtctcga
     4801 agtactcgcg cgtgccctcg cagagcacgc cgtcgaagtc gagggcgagg atgtcgggac
     4861 gctcgtcggc cacggcgcga ttctacgctc gcgcgcagga ctggctccag gatctttccg
     4921 aagcctgccc ggtggcgatg ccggcgggcg tgctaccatg cgccgatcga tcggcaggtc
     4981 gctccggcga cccggtccag tcggcatgag ccaacccgac gcattcttcg tccccgatgg
     5041 cgacgaccgc ttcgtcgcca cggagcacgg ccgcggtccc tggagcctgg accacctgca
     5101 tgccgggccg ccgtccgccc tgctggcgcg ggccatcgag cgcgccggcg ccggagatcc
     5161 cgtcgtcgtc actcgcctca cggtggacct gcttgccccc gtgcccatcg gcccgctcga
     5221 ggtggcgacg tccacgctcc gcgccggccg cacggtgcgg cgcctggagg cgacgctcgc
     5281 ggcaggcggg cgcgttctgg cccgcgcctc cggtctcgtc acccgcaccc aggccctgga
     5341 cttccctccg gtgaagcccg agccttgcga ccctcccccg cctcccgggt cgggagcacc
     5401 cttccggttc cccgtcttcc acggcagcgc gatcggctac cagaccgccg ttgacgcgcg
     5461 gtgcgtgcgc ggcggcttcg gccagaaccc gacgacggtg tggatccggg ctcgcgtgcc
     5521 cctcgtgctc ggcgaagcgc cgagcccgct cgcgcgcgtg atgatcgccg ccgactcggg
     5581 caacggc
//
LOCUS       DATIAZ010000156         8187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1224813, whole genome shotgun sequence.
ACCESSION   DATIAZ010000156 DATIAZ010000000
VERSION     DATIAZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224813"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1287)
                     /locus_tag="VLF19_05445"
     CDS             complement(112..1287)
                     /locus_tag="VLF19_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HSE92730.1"
                     /translation="MYPHQSERLTAALDEAGLDALVATTPANIVYVTGFQSLIQATYP
                     GVELYAVFSRGGTALVLPAADAVTAAVESVDVDHVSCYGRLVLGEARRADDVGRRARE
                     WTREPAPGPADALAAALDAVRAAGGRIGVDEAGLRPGAWRRVVERLAGRTVVEGAGAL
                     AGARRVKGPWEIECLQRALHVTEEAANEVIQALEPGMTEREAAARFLNEVITRGAEPR
                     GTVLLFGERTAFPAVGPSDRALRTGDLVRLDVGCVLKGYHAQVARTAVAGKPSDDQQR
                     AHAAIQSGLEAGLEAVRPGVTAGAVFDRAVDAARAAGLLQFDREQIGYGIGLEPSESP
                     KLTAGNGAQLQTGMVLRIDTPYYEPGWGGVHLGDTVLVTRNGHAVMNRSARGLVVLD"
     gene            complement(1352..1804)
                     /locus_tag="VLF19_05450"
     CDS             complement(1352..1804)
                     /locus_tag="VLF19_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92731.1"
                     /translation="MQLTPTAAHEGRVAAQNAVQGDHATPDLSVVPQTIFTTPEIARV
                     GLSHREAEARGLACHVARHDVAGASNGVATGEDGGFLKLVFEAATERILGVQMVSYAA
                     SELIQLAALAVRVGATADTLTTQLSIHPSHAERFIKVAAHEFHEVCEL"
     gene            complement(1784..2737)
                     /locus_tag="VLF19_05455"
                     /pseudo
     CDS             complement(1784..2737)
                     /locus_tag="VLF19_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(2846..3547)
                     /locus_tag="VLF19_05460"
     CDS             complement(2846..3547)
                     /locus_tag="VLF19_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92732.1"
                     /translation="MTLAGRIALVTGGTGALGQAVTLRFLAEGAVACVPWVVDEEHER
                     LRAAVKPADAGRLFLDRCDVTDDVALGRFVEAARGRHGAIDVLVTLVGGFAGGGLVEA
                     DRTAWDRMLALNLTSAFTATRAVVPAMVAARRGRVITIGSRAVLPPAGGFIAYTTSKA
                     GVIAFTQALAQELHGTGVTANCVLPSTMDTPGNRAAMPDADRKGWVPVESVAAAIAFL
                     ASDEARHVNGTLVSV"
     gene            complement(3558..4469)
                     /locus_tag="VLF19_05465"
     CDS             complement(3558..4469)
                     /locus_tag="VLF19_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HSE92733.1"
                     /translation="MADWATFPREEPPKPARDLADQVARDGGHVLAVYQEPVGQHWQL
                     FCLLPHEKVEASPYQRDLSPTHAKRLTDAMKKLDRFLDPVVALSPKPGVYWTPNGNHR
                     RMALEKLKAKWIPAILVVEPEVGYQILPLNTEKAHNLKEKSLEVIRMYRALAADEPSA
                     TEEAWAFQFESPHFITLGLLYEGNKRFAGGAFAPILRRVDRFLKLSLRKGLEERQERA
                     DLVRETDEALGVVVAKIKKRGINHPYVKNYVLARTTPLTRARKTLPSFEQTFKKLKES
                     LADFDVGKVRYEDIQRSAIMAAPAPSE"
     gene            complement(4481..5470)
                     /locus_tag="VLF19_05470"
     CDS             complement(4481..5470)
                     /locus_tag="VLF19_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92734.1"
                     /translation="MRRALPLAALTALAAAPFVPMGGMRQYVLHVIIQILVWSFIGQA
                     WSLMGRFGLVSLGHGAFLGVGAYATTLLWNAYGLTPWVGIVVAVAAAVALALVVAYPC
                     SRFGIVGHYFALVTLAVGEVVRLLIIAERDWTGGSLGLTLKAPESAASLFALQYADKR
                     VFYYIALAVWLGGLWAWRRLDRSMARAAMEAIGEDETAAASVGIHVTRFKMGVTALSA
                     ALTACGGVLYAQYITYVNPETLAGIGVSLRIVFAVVLGGMYSLLGPTVGTAITIALSE
                     YLRVVFGLRLIGMAETIYGLLLVLFIIFLPAGIWGSLRERLWRRRRSEAAATA"
     gene            complement(5470..6336)
                     /locus_tag="VLF19_05475"
     CDS             complement(5470..6336)
                     /locus_tag="VLF19_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92735.1"
                     /translation="MDPIFLAEAAVNGLLLGGVLALLALGLNLIFGVLDIVWIAYVDL
                     VMLCMYLVYFLVMTQGWPVWLAGVAGLAFGALLGVGVHWLIITPILTSPPVNQLLATG
                     GLLFFIQSFATFLWTTDHRSVRVALPSIEVGGIFLSFARLLAFVLAILAMLGLYVFLT
                     RTFIGTAIRAVAQDRGAMALMGASPQRIYTVTSAVGGAMAGAAAVLLSIQYSVHPHFG
                     AAFGPITFMICVLGGLGNMVGAFIAAFILSELISVGGVIWSTEMGYVIAFALFIVMMF
                     IRPGGILARRER"
     gene            complement(6336..7040)
                     /locus_tag="VLF19_05480"
     CDS             complement(6336..7040)
                     /locus_tag="VLF19_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92736.1"
                     /translation="MLELDRVTAGYGQFTALWDVSLRVTRGEAVAVVGPNGAGKTTLL
                     RVISGVIAPRRGDLVFEGDSLAGQPAHRIVAHGIAHVPEGRRLFPMLTVAENLKMGAF
                     LPAARRAFRESVERVYALFPVLAERQKQRAGSLSGGEQQMLAIGRALMSRPRLILFDE
                     PSMGLAPVMVLRLFDLIRRVREEGYTILVVEQNVRQVLKLVDRAYLLEVGRIKMEGRA
                     DELSEQDFVRKAYVGL"
     gene            complement(7040..7771)
                     /locus_tag="VLF19_05485"
     CDS             complement(7040..7771)
                     /locus_tag="VLF19_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92737.1"
                     /translation="MLTVSGLTKRFGGFTALNGVSFEVQQGEILGLIGPNGSGKTTCF
                     NCISGALTPSAGSLRFLGDEIAGLTPDAVCHRGIARTFQIPRPFRKLSILENVAVAAH
                     FGVQQRQTEAAALEQARRMLALVGLPADPATSTTLLGAGGLKKLELARALATRPRLLL
                     ADESLGGLDPAEMASAADMLRRVRGELGITVVWVEHIMATLMRVVDRVVVLDHGEKIA
                     EGRPAEVAEDPKVIEAYLGEKIVLA"
     gene            complement(7925..>8187)
                     /locus_tag="VLF19_05490"
     CDS             complement(7925..>8187)
                     /locus_tag="VLF19_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92738.1"
                     /translation="QKHGGFGAEALAKAARETDIAEGGTMQGYGVKFNPPGHAMAGQN
                     ARALAAVFQVVDGEFKHVYPPVVASMASPPVLLPASSPFAAR"
BASE COUNT         1198 a         2950 c         2984 g         1055 t
ORIGIN      
        1 tcactgttcg ggctctgcac cgagcgcagg ccggccccgg cgatcgctgg tgggatcgat
       61 gccggggtct gggatcaaac cggtcgggtc agctcggggc cccccatgta atcagtccag
      121 gacgaccagc ccccgtgcag agcggttcat cacggcatgg ccgttgcgcg tgacgaggac
      181 agtgtccccg aggtgcacac cgccccagcc gggctcgtag tacggcgtgt cgatcctcag
      241 caccatgccg gtctggagct gggcgccgtt gccggccgtc agcttgggac tctcggaggg
      301 ctcgagcccg atcccgtacc cgatctgttc gcggtcgaac tgtagcaggc ccgccgcgcg
      361 cgccgcgtcg accgcgcggt cgaagacggc gccggccgtc accccgggcc ggacagcctc
      421 gaggccggcc tccagcccgg actggatcgc ggcgtgagcg cgttgctggt cgtcggacgg
      481 cttgcccgcg accgcggtgc gggcgacctg cgcgtgatag cccttgagga cgcaacccac
      541 gtcgagcctg acgagatcgc ccgtccggag cgcgcggtcc gacgggccga cggcgggaaa
      601 ggccgtccgc tcgccgaaga gcagcacggt tccgcgcggc tcggcgccgc gcgtgatgac
      661 ctcgttgagg aaccgcgcgg ccgcctcccg ctcggtcatg ccgggctcga gcgcctggat
      721 cacctcgttc gccgcctcct cggtcacgtg cagcgcgcgc tgcaggcact cgatctccca
      781 gggccccttc acgcggcggg ccccggcgag cgcaccggcg ccttcgacca ccgtccgtcc
      841 ggcgaggcgc tcgacgacgc ggcgccaggc gcccgggcgc agaccggcct cgtccacgcc
      901 gatgcggcct ccggcagcgc ggacggcgtc gagggcggcg gccagcgcgt cggccgggcc
      961 cggcgccggc tcccgggtcc actcgcgcgc gcggcggccg acgtcgtcgg cccgccgggc
     1021 ctcgcccagc accagccgcc cgtagcagct cacgtgatcg acgtcgacgc tctcgacggc
     1081 cgccgtgacc gcgtcggccg ccggcagcac gagtgccgtg ccgccccggc tgaagaccgc
     1141 gtacagctcc acgccgggat aggtggcctg gatcaggctc tggaacccgg tgacatagac
     1201 gatgttcgcg ggcgtcgtcg ccacgagcgc gtcgagcccg gcctcgtcca gcgccgcggt
     1261 cagccgttcg gactggtgcg gatacacggt gtcgtcgtcc tcccccggcg cgcccgggtc
     1321 atcggacccc ccggcagcgt cgtcctgctg ctcacagctc gcagacctcg tggaactcgt
     1381 gcgcggcgac cttgatgaag cgctcggcgt ggctcgggtg gatcgagagc tgcgtggtca
     1441 gggtgtcggc cgtcgccccc acgcggacag cgagcgcggc gagctggatc agctccgacg
     1501 cggcgtacga gaccatctgc acgccgagga tgcgttccgt ggccgcctcg aagacgagct
     1561 tgaggaaccc gccatcctcg ccggtggcga cgccgttgga ggcgcccgcc acgtcgtggc
     1621 gcgcgacgtg gcacgcgagc ccgcgcgcct cggcctcgcg atgggaaagt cccacccggg
     1681 cgatctcggg cgtcgtgaag atcgtctgcg ggaccacgga caggtcgggg gtcgcgtggt
     1741 cgccctgcac ggcgttctgc gcggccacgc gcccctcgtg cgcggccgtc ggcgtgagct
     1801 gcacgttgcc ggcggcgtcg ccggcagcgt agacgtgcgg gagcgacgtg cgcaggtggc
     1861 gcgtggtcgc gaggccgagg cggcccgtct cgagccccag cgcttcggcg cccagcatcg
     1921 gcgggtggaa ccgccggccg atggccagac agacggccgt ggcgcgggtc tcgcgcgccc
     1981 gcgcctcctg ggcgaaccgg accgtgatgt cgccgcggcg gccggcgagg gcggtcaccc
     2041 gcgcgtcccg gtggaaggtg atgccgcggc cctcgaggat cgcgcgcagg taggccgcga
     2101 cgtcggcgtc gaagccggcc aggatctcgt tctcctgggc gaccaccgtc acctccgcgc
     2161 ccaggtcgct gaacgcggcg gccatctcga gcccgactgc gcccgcgccg atcaggacga
     2221 ggctcgccgg gaactcgggg aggaagagca gctcctccga cgtgatggcc agctcgcggc
     2281 cctcgatggg cggcacgacg ggcgccgagc cggccgcgat gatgatcttc tcgccgcgca
     2341 ggcggatgcc gccgaccgcg agcgtgtgcg gatcctcgaa gcgcgcctcg ccgaggacga
     2401 tccgcgcgcc ggtgcgctcg agcttctgca cggggggacg cagccgcgcc accgtgtcgt
     2461 gctggcgggc gacgacggcc gcccagtcca ggcgcgccgc gccggtggcc gtgccgaaga
     2521 cggcagcgtg gcgcacgagg cgatgcacgc ggccggccct gaccagggcc ttcttcggga
     2581 ttcagccgcg gttgatgcag gtgcctccga gcggcccgcg atcgaccagc gcgaccgagg
     2641 cgcccaggtc gcgggcggtc ttcatagcgt tgatcccggc ggcgccgccg ccgacgatca
     2701 cgacgtcgta gcgatccgtc atgtcgaggc tccccatgaa agcctgcacc cgcgaacgag
     2761 gcgcgccacg gctacgccgg ggcgggccga gccacccccg ggggctcggg gggccccgtc
     2821 gaggcccccc ttggagtcga tccgatcaaa ccgacaccag cgtgccgttg acgtggcgcg
     2881 cctcgtcgct cgccaggaag gcgatggcgg cagcgacgct ctcgacgggg acccagccct
     2941 tccggtcggc gtccggcatc gccgcgcggt tgccgggcgt gtccatcgtg ctcggcagca
     3001 cgcagttcgc ggtgacgccg gtgccgtgca gctcctgggc gagcgcctgc gtgaaggcga
     3061 tgacgccggc cttcgacgtc gtgtaggcga tgaagccgcc ggcgggcggc agcaccgcgc
     3121 gcgagccgat ggtgatcacg cggccgcggc gggccgcgac catcgcgggc acgacggccc
     3181 gggtggccgt gaacgcgctg gtgaggttga gcgcgagcat ccggtcccac gccgtccggt
     3241 cggcctcgac caggccgccg ccggcgaagc cgccgacgag ggtgacgagc acgtcgatcg
     3301 cgccgtggcg tccgcgcgca gcctcgacga accggcccag cgcgacatcg tcggtgacgt
     3361 cgcagcggtc caggaagagg cggcccgcgt ccgccggctt caccgccgcc cgcagccgct
     3421 cgtgctcctc gtccaccacc cacggcacgc acgcgacggc gccctcggcc aggaaccgca
     3481 gcgtgaccgc ctgccccagc gctccggtgc cgccggtgac gagcgcgatg cgcccggcca
     3541 gcgtcaccgc cgcgccctca ctcgctcggc gccggcgcgg ccatgatggc cgagcgctgg
     3601 atgtcctcgt agcgcacctt gcccacgtcg aaatcggcga ggctctcctt cagcttcttg
     3661 aaggtctgct cgaaggacgg cagcgtcttg cgcgcgcgcg tgagcggcgt cgtgcgcgcc
     3721 agcacgtagt tcttcacgta ggggtggttg atgccgcgct tcttgatctt ggccaccacc
     3781 acgccgaggg cctcgtccgt ctcgcggacg aggtccgcac gctcctggcg ctcctcgagc
     3841 cccttgcgca gcgacagctt gaggaacctg tccacgcggc ggaggatcgg cgcgaaggcc
     3901 ccgccggcga agcgcttgtt gccctcgtag aggaggccga gcgtgatgaa gtggggcgac
     3961 tcgaactgga acgcccacgc ctcctcggtg gccgagggct cgtccgccgc cagcgcccgg
     4021 tacatgcgga tgacctcgag cgacttctcc ttgaggttgt gcgccttctc ggtgttgagc
     4081 ggcaggatct ggtagccgac ctccggctcg acgacgagga tggccgggat ccacttcgcc
     4141 ttgagcttct cgagcgccat ccgccggtgg ttgccattgg gcgtccagta gacccccggc
     4201 ttcggggaca gcgccaccac ggggtccagg aagcggtcga gcttcttcat ggcgtcggtc
     4261 agccgcttgg cgtgggtggg cgagaggtcg cgctggtacg ggctcgcctc gaccttctcg
     4321 tgcggcagca ggcagaagag ctgccagtgc tggcccaccg gctcctggta gacggccagc
     4381 acgtggccgc cgtcgcgcgc cacctggtcg gccaggtcgc gcgcgggctt cggtggctcc
     4441 tcgcggggga acgtcgccca atcggccatg gccgtctccc tcaggccgtc gcggccgcct
     4501 cgctccgccg ccggcgccac agccgctcgc gcaggctgcc ccagatgccg gcgggcaaga
     4561 agatgatgaa gagtaccagc agcaggccgt agatggtctc ggccatgccg atgagcctga
     4621 ggccgaacac cacgcgcagg tactcggaca gggcgatcgt gatggccgtg cccaccgtgg
     4681 gcccgagcag cgagtacatg ccgcccagca ccacggcgaa cacgatgcgc agcgacacgc
     4741 cgatgccggc cagcgtctcg gggttcacgt aggtgatgta ctgcgcgtac agcacgccac
     4801 cgcacgccgt gagcgccgcc gagagcgcgg tgacccccat cttgaagcgc gtcacgtgga
     4861 tgcccaccga ggccgcggcc gtctcgtcct cgccgatggc ctccatggcc gcgcgcgcca
     4921 tcgaccggtc gagccggcgc cacgcccaga ggccgccgag ccagacggcc agcgcgatgt
     4981 agtagaagac ccgcttgtcg gcgtactgca gggcgaagag gctcgccgcg gactccggcg
     5041 ccttcagcgt gagccccagc gagccgcccg tccagtcgcg ctcggcgatg atgagcaggc
     5101 gcacgacctc gccgacggcc agggtgacga gggcgaagta gtggccgacg atcccgaagc
     5161 gcgagcaggg gtaggcgacc acgagggcca gcgcgacggc cgcggccacg gcgacgacga
     5221 tgcccaccca cggcgtcagc ccgtaggcgt tccagagcag cgtggtcgcg taagcgccga
     5281 cgccgaggaa ggcgccgtgg ccgagcgaga cgagcccgaa ccgtcccatg agcgaccacg
     5341 cctgcccgat gaacgaccag acgaggatct ggatgatgac gtgcagcacg tactgccgca
     5401 tgccccccat cggcacgaag ggcgccgccg cgagcgcggt cagggcggcg agcgggagcg
     5461 cccggcgcat caccgctccc gccgggccag gatcccgccg ggacggatga acatcatcac
     5521 gatgaagagc gcgaaggcga tgacgtagcc catctcggtg gaccagatga ccccgcccac
     5581 ggagatgagc tcgctgagga tgaacgcggc gatgaaggcg ccgaccatgt tccccagccc
     5641 gccgagcacg cagatcatga aggtgatggg accgaacgcc gccccgaagt gcgggtgcac
     5701 ggagtactgg atggacagca gcaccgcggc cgcgccggcc atggcgccgc cgacggccga
     5761 ggtgacggtg tagatgcgct gggggctcgc ccccatgagg gccatggctc cgcggtcctg
     5821 ggcgacggcc cggatggcgg tgccgatgaa ggtgcgcgtg aggaacacgt agaggcccag
     5881 catggcgagg atcgccagca cgaaggccag gagccgcgcg aacgacagga agatgccgcc
     5941 cacctcgatg ctgggcagcg cgacgcgcac ggagcggtga tcggtggtcc acaggaacgt
     6001 ggcgaacgac tggatgaaga agagcagccc gcccgtggcc aggagctggt tcaccggcgg
     6061 gctcgtgagg atcggcgtga tgatgagcca gtgcacgccg acgccgagca gcgcgccgaa
     6121 ggcgagcccg gccacgccgg ccagccacac cggccagccc tgggtcatga ccaggaagta
     6181 gacgaggtac atgcacagca tcacgagatc gacgtaggcg atccagacga tgtcgagcac
     6241 gccgaagatc aggttgagcc cgagcgcgag cagcgccagc acgccgccca gcagcaggcc
     6301 gttcacggcc gcctcggcga ggaagatggg gtcgatcaca ggcccacgta ggccttccgc
     6361 acgaagtcct gctcggagag ctcgtcggcc cggccctcca tcttgatgcg gccgacctcc
     6421 aggaggtagg cgcggtccac gagcttgagg acctgccgca cgttctgctc gaccacgagg
     6481 atggtgtagc cctcctcgcg cacgcggcgg atcaggtcga agaggcgcag gaccatgacc
     6541 ggggccaggc ccatcgaggg ctcgtcgaag aggatcagcc tggggcgcga catgagcgcg
     6601 cgcccgatgg cgagcatctg ctgctcgccg ccggagaggc tgcccgcccg ctgcttctgg
     6661 cgctcggcca ggacggggaa gagggcgtac acgcgctcga cgctctcgcg gaaggcgcgg
     6721 cgcgcggcgg gcaggaacgc gcccatcttg aggttctcgg cgacggtgag catgggaaag
     6781 agccgccggc cctcgggcac gtgggcgatg ccgtgggcga cgatccggtg cgcgggctgg
     6841 cccgcgagcg agtcgccctc gaagacgagg tcgccgcggc gcggcgcgat gacgccggag
     6901 atcacgcgca ggagcgtggt cttgccggcg ccgttcgggc ccacgacggc gacggcctcg
     6961 ccgcgcgtca cgcgcaggct cacgtcccac agtgccgtga actgtccgta ccccgccgtg
     7021 acccggtcca gctcgagcat caggccagca cgatcttctc gccgaggtag gcctcgatca
     7081 ccttcgggtc ctcggcgacc tcggcggggc ggccctcggc gatcttctcg ccgtgatcca
     7141 gcacgaccac gcggtccacg acgcgcatga gcgtggccat gatgtgctcg acccagacca
     7201 ccgtgatccc cagctcgccc cgcacgcggc gcagcatgtc ggccgcgctc gccatctccg
     7261 cggggtcgag gccgcccagg ctctcgtcgg cgagcaagag cctgggcctc gtcgccagcg
     7321 cgcgggccag ctcgagcttc ttgagcccgc cggccccgag cagcgtggtg gaggtcgcgg
     7381 ggtcggccgg cagcccgacc agcgccagca tgcggcgggc ctgctcgagc gccgcggcct
     7441 cggtctgtcg ctgctggacg ccgaagtgcg cggccaccgc cacgttctcg aggatcgaca
     7501 gcttgcggaa cggccgcggg atctggaagg tccgcgcgat cccccggtgg cacaccgcgt
     7561 cgggcgtcag gccggcgatc tcgtcgccga ggaagcggag gctgccggcg ctcggcgtga
     7621 gcgcgccgga gatgcagttg aagcacgtcg tcttccccga cccgttcggc ccgatgagac
     7681 ccaggatctc gccctgctgc acctcgaacg acacgccgtt gagcgcagtg aatcccccga
     7741 accgcttcgt cagcccggaa acggtcagca tcggcccggc ccaggccgcg gatcactgcg
     7801 caccgaatcg atggacggag tcgagcggag gggacgtctc gacccctccg atccggtcga
     7861 tccgccggct gtgccggcgc agtcgggcct ggggagagac attagagggg ggcgaagccc
     7921 cccgctaacg cgcggcgaac ggcgaggagg ccgggagcag cacgggcggc gacgccatgc
     7981 tcgccaccac cggtgggtac acgtgcttga actcgccgtc caccacctgg aacacggcgg
     8041 cgagcgcgcg cgcgttctgg ccggccatcg catggccggg cgggttgaac ttgacgccgt
     8101 agccctgcat cgtgccgccc tcggcgatgt ccgtctcgcg cgcggccttg gccagcgcct
     8161 cggcgccgaa cccgccgtgc ttctgga
//
LOCUS       DATIAZ010000157         4557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_436134, whole genome shotgun sequence.
ACCESSION   DATIAZ010000157 DATIAZ010000000
VERSION     DATIAZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436134"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VLF19_05495"
     CDS             complement(<1..390)
                     /locus_tag="VLF19_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92739.1"
                     /translation="MTPEFWVAQTFSGLSYGALLFLLAGGLSLIFGMMRIVNMTHGSY
                     YLLGGYVALTTIWRTERFELGLVAAALAIAFIGVCEWNAFLKRLSGQELGQVLTTMGF
                     ALIFQDLALVIWGGDPYTIPIPKILDGS"
     gene            complement(422..1423)
                     /locus_tag="VLF19_05500"
     CDS             complement(422..1423)
                     /locus_tag="VLF19_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010212491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE92740.1"
                     /translation="MTPPLGIDIHAHFFPESYLRVIEKEGASAGARIDRANPAGPAVV
                     IAGVSTPPLVPAYWDIDARLKAMNRTGVAVQALSLTVPMVYFAGSALARRLAQAFNDA
                     CAEAHTAHPSRFVGCATLPMQEPAAALAELERAARLPGVRAVYLGTNVNGRELSDPAF
                     ASIFERCQEYELPLLLHPISVVGASRLQPFYLSNLLGNPFDTAVAAAHLVFGGVLDRF
                     PRLQVCLPHAGGAMPYLFGRLQHGQRVRPETRERARRPFSAYLRRFTYDTISHSPEAL
                     RYLVDLVGVDRVMLGSDFCFDMGYERPRDIVTKRLRLKAGDQARIIRGNAARLLGLA"
     gene            1562..2479
                     /locus_tag="VLF19_05505"
     CDS             1562..2479
                     /locus_tag="VLF19_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSE92741.1"
                     /translation="MKLPHERFDYSPIADRRPWTLPEGARIAVWTIVNVEEWSIEKAM
                     PRQYLTTPQGVTVVPDVPNWAWHEYGMRVGFWRLLEALGKRRLRATTSINAHVCEAYP
                     AVARAMLEAGWEFMGHGVVQGAMHLLPDQRAAIRESIRLLEKFTGKRPKGWLGPGLTE
                     TWETLDLLAEEGIEYVSDWVNDDQPYEIRTSTRPLVSVPYTIELNDIPMMVIQHHESA
                     YWLQRAKDQFDRLYAEGARNPRVMALAVHPYISGVPHRIKYFEAVYDYVMRKKGVWLT
                     TGEEICEWYRQSRSEPTSPARPPRRSGRP"
     gene            complement(2436..3413)
                     /locus_tag="VLF19_05510"
     CDS             complement(2436..3413)
                     /locus_tag="VLF19_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92742.1"
                     /translation="MTAAVSISVPDDFPSVFDGTPAHARARALGEVRLFTARGADDEA
                     ELIRRIDRARVVINIRAHARFTEGVFAACKSLELVSIWGTGTDNVDLGAAGRHGVTVC
                     NTPGVNAHAVAEHTLALMLAVARRIPAIDREVRAGHWPRELLTQLLGKTLGVFGLGGI
                     GARVAELGRAVGMSVLGWSLRGDEARVKALGARPASKEEILREADVISLNLRLIPETR
                     GFIGRREFALMKPSAILVNTARGALVERDALLEALSRSRIAGAGLDVFHDEPVKGADP
                     LLKLDNVVLSPHNAGQTPEVIRDGLLRAVQNVENFLKGAPTDVVVAPAK"
     gene            complement(3410..>4557)
                     /locus_tag="VLF19_05515"
     CDS             complement(3410..>4557)
                     /locus_tag="VLF19_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSE92743.1"
                     /translation="PPVIQALNQPPAIQYDRSFDEGVLEPTTLALRHVFQTRNEVILM
                     PGSGRTALEAGALSVIEPGERALVVAAGQFGILMREIMHRVGAELTEFPVEPGRALDL
                     ARLTAEARRLRPKAITLVHNETSTGTTYPAAEVGKIAREVGALFMLDTVSSIAGIDVR
                     TDEWGVDLNMTGSQKCLAAPLGLALVAVSPRAWEAMERRTRPATSYAYDLLRWKEHWI
                     PESRGGRVKDGAPRRQPVSIPTHLTGAMQVAVRLILEEGLPHRFRRHEVAAKALRAGV
                     VAMGLDMFPDASLLSNTVSSIKAPRDVQTGAVVTRMREQYGILIGTGLDSLRTTTLRV
                     GTMGITASPPYVLPTLAALELALRDLGYKAEPGAGVAAAQQVFADHS"
BASE COUNT          692 a         1569 c         1666 g          630 t
ORIGIN      
        1 ggagccgtcg aggatcttcg ggatcgggat cgtgtaggga tcgccgcccc agatgacgag
       61 cgcgagatcc tggaagatga gcgcgaaccc catcgtcgtg agcacctggc ccagctcctg
      121 accgctcagg cgcttgagga acgcgttcca ctcgcagacg ccgatgaacg cgatggccag
      181 tgccgcagcc acgagcccga gctcgaagcg ctccgtccgc cagatcgtcg tcagcgccac
      241 gtacccgccg agcaggtagt aggacccgtg ggtcatgttg acgatgcgca tcattccgaa
      301 gatgagcgac agcccgcccg cgagcaggaa caggagggcg ccgtaggaaa ggccgctgaa
      361 cgtctgggcg acccagaact ccggcgtcac gccgcctccg ccaggccccc cggcactctc
      421 ctcacgccag gcccagcagg cgcgcggcgt tgccgcgaat gatgcgcgcc tggtcgccgg
      481 ccttgaggcg cagccgcttg gtgacgatgt cgcgcggccg ctcgtagccc atgtcgaagc
      541 agaagtcgct gccgagcatc acccggtcca cgccgacgag gtccaccagg tagcgcagcg
      601 cctccggcga gtggctgatg gtgtcgtagg tgaaccggcg cagataggcg ctgaaggggc
      661 gccgcgcgcg ctcgcgcgtc tcgggccgca cgcgctggcc gtgctgcagc cgtccgaaga
      721 ggtagggcat ggcgccgccc gcgtggggca gacacacctg gagccgcggg aaacggtcga
      781 ggacgccccc gaagacgagg tgcgcggcag ccaccgcggt gtcgaacggg ttgccgagca
      841 ggttgctcag gtagaacggc tgcagtcgcg aggcgccgac gacgctgatc gggtggagca
      901 gcagtggtag ctcgtactcc tggcagcgct cgaagatcga cgcgaaggct ggatccgaca
      961 gctcccgccc attgacgttg gtgcccaggt acacggcgcg cacgcccggc aggcgtgcgg
     1021 cgcgctccag ctcggcgagc gccgccgccg gctcctgcat cggcagcgtc gcgcagccga
     1081 cgaaccggct ggggtgcgcg gtgtgggcct ccgcgcacgc gtcgttgaag gcctgggcca
     1141 gacgccgcgc cagcgcgctg ccggcgaagt acaccatcgg caccgtgagc gagagcgcct
     1201 gcacggccac accggtgcgg ttcatcgcct tgaggcgcgc gtcgatgtcc cagtacgcag
     1261 gcacgagcgg cggcgtcgac acgccggcga tgacgacggc ggggcccgcg ggattcgcgc
     1321 gatcgatgcg ggcgccagcc gacgcgccct ccttctcgat cactcgcaga tagctttccg
     1381 gaaagaagtg cgcgtggatg tcgatgccga gggggggcgt cacggcgcag gattgtacta
     1441 tagccgcccg ccggatgtac catgccgggt cagtttcgat cactcgcccg cgcgcgggcg
     1501 ggcccggccc gcccgggtcg ctgggccatc gaagatcagg cccggcccca aggaggcgcc
     1561 cgtgaagctg ccccacgaac ggttcgacta ttccccgatc gccgaccgcc gcccctggac
     1621 gctccccgag ggcgcgcgga tcgccgtatg gaccatcgtc aacgtcgagg aatggtccat
     1681 cgagaaggcc atgcccaggc agtacctcac gacgccgcag ggggtgaccg tggtgcccga
     1741 cgtgccgaac tgggcgtggc acgagtacgg gatgcgagtg ggattctggc ggctgctgga
     1801 ggccctcggc aagcggcggc tccgcgcgac gacctcgatc aacgcccacg tgtgcgaggc
     1861 ctaccccgcc gtcgcccggg ccatgctcga ggccggctgg gagttcatgg gccacggcgt
     1921 ggtgcagggg gccatgcacc tcctgcccga tcagcgggcg gccatccggg agtcgatccg
     1981 cctgctcgag aagttcaccg gcaagcggcc caagggctgg ctcggtcccg gcctcaccga
     2041 gacgtgggag acgctcgacc tgctcgccga ggaggggatc gagtacgtgt cggactgggt
     2101 caacgacgac cagccgtacg agatccgcac ctcgacccgg ccgctggtgt ccgtgcccta
     2161 cacgatcgag ctgaacgaca tccccatgat ggtcatccag caccacgagt cggcgtactg
     2221 gctgcagcgc gcgaaggacc agttcgaccg gctctatgcc gagggcgcga ggaatccccg
     2281 cgtgatggcc ctggcggtgc acccctacat ctcgggcgtg ccgcaccgga tcaagtactt
     2341 cgaggccgtc tacgactacg tgatgaggaa gaagggcgtc tggctcacca ccggcgagga
     2401 gatctgcgag tggtatcgcc agtcgcgcag cgagcctact tcgccggcgc gaccaccacg
     2461 tcggtcgggg cgcccttgag gaagttctcg acgttctgga cggcccggag caggccgtcg
     2521 cgaatgacct cgggcgtctg cccggcgttg tgcggcgaga gcacgacgtt gtcgagcttg
     2581 agcagcgggt cggccccctt gaccggctcg tcgtggaaga cgtcgaggcc ggcgccggcg
     2641 atgcggctcc ggctcagcgc ttcgagcagc gcgtcgcgct ccacgagcgc cccgcgcgcg
     2701 gtgttgacga ggatcgcgga gggcttcatc agtgcgaatt cccggcgccc gatgaagccg
     2761 cgggtctcgg ggatcaatcg caggttgagg gagatcacgt cggcctcgcg gaggatctcc
     2821 tccttggagg cgggacgggc gcccagcgcc ttcacccggg cctcgtcgcc gcgcaggctc
     2881 cagccgagca cgctcatgcc caccgcgcgg cccagctcgg cgacccgcgc accgatcccg
     2941 ccgaggccga acacgccgag cgtcttgccc aggagctgcg tcaacagctc gcgcggccag
     3001 tgtccggcgc gcacctcccg gtcgatcgcc gggatccggc gcgccacggc gagcatgagg
     3061 gccagcgtgt gctcggcgac cgcgtgcgcg ttcacgcccg gtgtgttgca gacggtcacg
     3121 ccgtggcggc cggcggcgcc gaggtcgacg ttgtccgttc cggtgcccca gatcgacacc
     3181 agctcgaggc tcttgcaggc ggcgaagacg ccttcggtga accgggcgtg cgcgcggatg
     3241 ttgatgacga cgcgcgcgcg gtcgatgcgg cggatcagtt cggcctcgtc gtcggcgccg
     3301 cgggcggtga agagccggac ctcccccagg gccctcgcgc gcgcgtgagc cggcgtgccg
     3361 tcgaagacgg acgggaagtc gtccgggacg gagatcgaca ccgccgcggt cacgagtggt
     3421 cggcgaacac ctgctgggcc gcggcgacgc cggcgccagg ctcggccttg tacccgaggt
     3481 cgcggagtgc cagctccagc gcggcgaggg tcggcagcac gtagggcggg ctcgcggtga
     3541 tgcccatggt gccgacgcgc agggtggtcg tgcggagact gtcgaggccg gtgccgatga
     3601 ggatgccgta ctgctcgcgc atccgcgtca ccacggcccc cgtctggacg tccctgggtg
     3661 ccttgatcga cgacacggtg ttggacagca gcgaggcgtc ggggaacatg tccaggccca
     3721 tggcgacgac accggcgcgc agcgccttcg ccgcgacctc gtggcgccgg aagcggtgcg
     3781 gcagtccctc ctcgaggatc aggcgcacgg cgacctgcat ggccccggtg aggtgggtgg
     3841 ggatcgacac cggctgacgt cgtggggcgc cgtccttgac gcggccgccg cgcgactccg
     3901 ggatccagtg ctctttccag cgcagcaggt cgtaggcata ggaggtggcg ggccgcgtgc
     3961 ggcgctccat cgcctcccag gcccgcgggc tcaccgccac cagcgccagc ccgagcgggg
     4021 ccgcgaggca cttctgcgag ccggtcatgt tgaggtccac gccccactcg tcggtgcgca
     4081 cgtcgatgcc cgcgatcgag gagaccgtgt cgagcatgaa cagcgcgccc acctcgcgcg
     4141 cgatcttgcc cacctcggcg gctggatagg tggtgccagt ggacgtctcg ttgtgcacga
     4201 gcgtgatggc cttgggccgg agccgacggg cctcggcggt gaggcgggcg aggtcgagcg
     4261 cccgccccgg ctccaccggg aactcggtga gctccgcgcc gacgcgatgc atgatctccc
     4321 gcatgaggat gccgaactga ccggcggcga cgacgagtgc ccgctcgccc ggctcgatca
     4381 ccgacagcgc gcccgcctcg agcgcggtgc ggccggagcc cggcatgagg atgacctcgt
     4441 tccgcgtctg gaacacgtgg cgcaacgcca gggtcgtcgg ctcgagcacg ccctcgtcga
     4501 acgagcggtc gtactggatc gccggcggct ggttgagcgc ctggatgacc ggcgggg
//
LOCUS       DATIAZ010000158         5949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_568178, whole genome shotgun sequence.
ACCESSION   DATIAZ010000158 DATIAZ010000000
VERSION     DATIAZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568178"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..948)
                     /locus_tag="VLF19_05520"
     CDS             complement(112..948)
                     /locus_tag="VLF19_05520"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92744.1"
                     /translation="MAPLDNAIRSEAPSDTPLAVELRGVGFDYRTDGRLTQAVQDVSL
                     GVPRGATVAFVGPSGCGKSTLLKLVAGLLRATRGAVVIDGWPVEAPLKNVGMAFQNPV
                     LLPWRTVLGNLLLPLEILRENGDRRAVDRALWEDRAVELLRTVGLAGAERRHPRELSG
                     GMRQRVSLCRALIHEPELLLLDEPFAALDAFTREEMWELHQRLRQGREFTGVLVTHDL
                     REAAFLARTIYVMSANPGRVAHVHEVDLAFPRALADVYSPVTTELIRLMREQIRPVAS
                     AT"
     gene            complement(1012..2031)
                     /locus_tag="VLF19_05525"
     CDS             complement(1012..2031)
                     /locus_tag="VLF19_05525"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92745.1"
                     /translation="MNVTCRRAALAAALLVAWAAPADAQTRVKFALDWQIQGPQAPFI
                     TAKASGAFTRQGLDVTIDRGTGSQKTIEQVATGTYDMGYGDINSMIEYNVKNPQTPLI
                     AVGIILNTPPFSLLSLKKYGIKEPKDLKGAAIGAPAGDAPRRLWPIFAKNAGVAVDSV
                     TWTNMAPPLREPALVKGDVKVISGFYFTGFLNLTENLKIPESEVVAFKYSDYGIDLYG
                     NAVVVRVDWLKKNEDTVRKFMAALNEGFKATLKDPKAAIVHVKAAEPLSNEATELKRL
                     QLSIAANMVNDEVKRNGLLGIDAARMQRAIDQVAQSFELPSKPAVAQVFTDAYLPPAS
                     ARKLD"
     gene            2148..4346
                     /locus_tag="VLF19_05530"
     CDS             2148..4346
                     /locus_tag="VLF19_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE92746.1"
                     /translation="MGVVGAAVRRTEGLDKVTGRTRYVDDLAVPGMLLGRTVRSSIAR
                     GWLDRIDFDLALPWGEIVVVTAAHIPGRNVVTLIEEDQPCLAQREIRHREEPVVLLAH
                     ADGDLLARAAEGVRLVVTSAPPVLALDDARDVLAEQTLARGDVETGLAEADLVVEATY
                     ETGAQEHVYIEPQGMIAEPRADGGMVLRGSLQCPYYVQPAVAAVLGVPAAMVQVIQTP
                     TGGGFGGKEEYPSMIACHAALLARKAGRPVKIVYERGEDMRATTKRHPSRTRHRLGVT
                     RDGRFVALETDFVLDGGAYVTLSPVVLSRGLIHAPGPYRWPSVRVRGRVLRTNHPPHG
                     AFRGFGAPQAIFALEAHVDRVARELGMDPVELRRRNLLRPGDATATGQHVDPGVDPVA
                     VLDTALRESAFERRRAAHAAANARSPHLRRGVGLTTFFHGAGFTGGGEVKLASVAGLA
                     LRPDADVEILASSAEIGQGAATTHVQIVAEALGLPHEMIHPPRVDTDLVPNSGPTVAS
                     RTVMVVGGLLAEAARKMLETLRAEGGLPAPHTADDFRAAARRRHAEHGPLSATAAYRP
                     REGWAWDEKALVGDAYAAYAWACYVAEVAVDVRTGEVEVTDFVAVQDVGRVINPVTAR
                     GQVHGGVTQGIGFALYEDVVWRDGVMANARLSDYILPTSADTPPIRAVFLEVPYAGGP
                     FGAKGLGELPMDGTAPAVVNAVNAALGTALARLPATPEHVLEAWQGTPHS"
     gene            4408..4902
                     /locus_tag="VLF19_05535"
     CDS             4408..4902
                     /locus_tag="VLF19_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSE92747.1"
                     /translation="MAGEIRVNDRMRVALNVNGAARVIEVSPVARLLDVLREVLRLTG
                     TKEGCGEGECGACSVLLDGALVVSCLVPIGQCDGASITTIEGLAPAGAGRLAPVQEAL
                     LDEGGTQCGICTPGIVVAAQALLSANPTPALADIREALAGNLCRCTGYTAIYEAIRRA
                     AGAR"
     gene            4946..5800
                     /locus_tag="VLF19_05540"
     CDS             4946..5800
                     /locus_tag="VLF19_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSE92748.1"
                     /translation="MTALGWVSARTLDEALARLADDPALTPIAGGTDVMVAARMGGLR
                     EGRLLDLWRLDELRGAEVARDSLLLGALTTYTEIVTHRAIATRWPLLAAAARVSGAWA
                     IRNRGTLGGNVANASPAADTPPALLAYGARLELRSARGARWVDYRSFHTGYRRTLRAP
                     DELITRIAVPAPPGDAVHFYRKVGARRAQAISKVCFAATAARRDGALADVRLALGSVA
                     PTVVSARRTAAWLEGRRPAAVDRAEARALLESEIAPIDDLRSTAGYRRRVAGNLLEQF
                     LADAAERP"
BASE COUNT          796 a         2122 c         2205 g          826 t
ORIGIN      
        1 cacctcccag accagcagca acacggtgaa cacgatggct ggcagcgcca cacggcgtat
       61 catggcgcgc tcgtcacccg gcggatcatt cggggctcct cacgtgaccg atcatgtggc
      121 gctcgccacc gggcggatct gctcgcgcat cagccggatc agctcggtcg tgacgggact
      181 gtacacgtcg gccagcgccc gcggaaacgc caaatccacc tcgtgcacgt gcgccacgcg
      241 cccggggttg gcggacatga cgtagatggt gcgagcaagg aacgccgcct cgcggaggtc
      301 gtgggtcacc agcacgccgg tgaactcgcg gccctgacgg agccgctggt gcagctccca
      361 catctcctcg cgcgtgaagg cgtcgagcgc ggcgaagggc tcgtcgagca gcagcagctc
      421 cggctcgtgg atgagcgcgc ggcagaggct cacgcgctgg cgcattccgc ccgacagctc
      481 gcgcggatgc cggcgctcgg cgccggcgag ccccaccgtg cggagcagct cgacggcgcg
      541 gtcctcccag agcgcgcgat ccacggcgcg gcggtcgccg ttctcgcgca ggatctcgag
      601 cggcaggagc aggttgccga gcaccgtgcg ccagggaagc aggacggggt tctggaatgc
      661 catgccgacg ttcttgagcg gcgcctcgac cggccagccg tcgatgacga cggcgccgcg
      721 ggtggcgcga agcagcccgg ccacgagctt gagcagtgtg gacttgccgc acccgctcgg
      781 cccgacgaac gccacggtgg cgccgcgcgg cacgccgagc gagacgtcct gcacggcctg
      841 cgtgaggcgt ccgtccgtcc ggtaatcgaa cccgaccccc cgcaactcga cagcgagcgg
      901 ggtgtcgctc ggcgcctcag aacgaatcgc gttgtcgagg ggcgccatgg ctgcgccgga
      961 gcgccccgag cgccgggggg catggggggc gccccgagcc cccccgtgta atcaatccag
     1021 cttccgggcg gacgccggcg gcaggtaggc gtccgtgaac acctgggcca ccgccggctt
     1081 cgacggcagc tcgaaggact gcgccacctg gtcgatggcc cgctgcatcc gggcggcgtc
     1141 gatgccgagc aggccgttgc gcttcacctc gtcgttgacc atgttcgcag cgatggacag
     1201 ctgcaagcgc ttgagctcgg tggcttcgtt ggagagcggc tcggcggcct tgacgtggac
     1261 gatggcggcc ttcgggtcct tcagcgtggc cttgaagccc tcgttgagcg cggccatgaa
     1321 cttccggacc gtgtcctcgt tcttcttgag ccagtccacg cgcaccacga cggcgttgcc
     1381 gtacagatcg atgccatagt cgctgtactt gaaggcgacg acctcgctct cgggaatctt
     1441 caagttctcg gtgaggttca ggaaacccgt gaagtagaag ccggagatga ccttcacgtc
     1501 gcccttgacc agcgcgggct cgcggagcgg cggcgccatg ttcgtccacg tcacgctgtc
     1561 caccgcgacg ccggcgttct tcgcgaagat gggccacagc cgccgcggcg cgtcgccggc
     1621 aggcgcaccg atcgcggcgc ccttgagatc cttcggctcc ttgatgccgt acttcttgag
     1681 cgagaggagc gagaagggcg gcgtgttgag gatgataccg accgcgatga gcggggtctg
     1741 cggattcttg acgttgtact cgatcatcga gttgatgtcg ccgtagccca tgtcgtaggt
     1801 gccggtggcc acctgctcga tggtcttctg cgagcccgtg ccgcggtcga tcgtgacgtc
     1861 gagcccctgc cgggtgaacg caccgctcgc cttcgcggtg atgaacggcg cctgcggacc
     1921 ctggatctgc cagtcgagcg cgaacttcac ccgggtctgg gcgtcggcgg gcgcagccca
     1981 cgcgacgagc agcgcggcgg cgagcgcggc gcggcggcac gtgacgttca tggcggctcc
     2041 tccaagcgac cggatcgccg ataaggtagg agagaccgtg cgccgcgtca accgcgcggc
     2101 tcacggcggc gaccttcgcg cgggcgccgt ccgtataatc gccgtccatg ggagtggtcg
     2161 gcgcggcggt ccgccgaacg gagggcctcg acaaggtcac gggccggacg cggtacgtgg
     2221 acgaccttgc cgtgccgggc atgctgctcg gccgcacggt gcgttcatcc atcgcacggg
     2281 gatggctcga tcggatcgac ttcgacctgg cgcttccgtg gggcgagatc gtcgtggtca
     2341 cggcggcgca catcccggga cgcaacgtgg tgacgctgat cgaggaggac cagccgtgtc
     2401 tcgcgcaacg cgagatccgc catcgcgagg agccggtggt cctcctggcg cacgccgacg
     2461 gcgacctgct cgcgcgcgcc gccgagggcg tgcggctcgt cgtgacatcg gcgccgcccg
     2521 tgctcgccct cgacgacgcg cgcgacgtcc tggccgagca gacgctggcg cgcggcgacg
     2581 tcgagaccgg cctggccgag gccgacctcg tggtcgaggc gacctacgag acgggcgccc
     2641 aggagcacgt ctacatcgag ccgcagggca tgatcgccga gccacgcgcc gacggcggca
     2701 tggtgcttcg cggctcgctc cagtgcccgt actacgtgca gccggcggtc gccgcggtgc
     2761 tcggtgtgcc ggcggcgatg gtccaggtga tccagacgcc gaccggtggc ggcttcggcg
     2821 gcaaggagga gtacccgtcg atgatcgcct gccacgcggc gctgctcgcg cgcaaggccg
     2881 ggcgccccgt gaagatcgtg tacgagcgcg gcgaggacat gcgcgcgacc accaagcgtc
     2941 acccctcgcg cacgcgccac cgcctcggcg tcacgcgcga cggccgcttc gtcgcgctcg
     3001 agacggactt cgtgctcgac ggcggcgcct acgtcacgct gagccccgtc gtgctgtcgc
     3061 ggggcctcat ccatgcgccg gggccgtatc ggtggccgag cgtgcgcgtg cgtggccgcg
     3121 tgctccgcac gaaccatccc ccgcacggcg cgttccgcgg cttcggcgcg ccgcaggcga
     3181 tcttcgccct cgaggcgcac gtcgatcgcg tggcgcgcga gctcggcatg gatcccgtcg
     3241 agctgaggcg gcgcaacctc ctccgtcccg gagacgcgac ggcgaccggc cagcacgtgg
     3301 acccaggtgt cgatcccgtg gccgtcctcg acaccgcgct gcgcgagagc gccttcgagc
     3361 gccggcgcgc agcccacgcc gccgccaacg cgcgctcgcc ccatctgcgg cgcggggtcg
     3421 ggctgacgac gttcttccac ggcgccggct tcaccggcgg cggtgaggtg aagctggcct
     3481 cggtcgcggg cctcgcgctg cgccccgacg ccgacgtgga gattctcgcc tcgagcgccg
     3541 agatcgggca gggcgcggcg acgactcacg tgcagatcgt cgccgaggcg ctgggtctgc
     3601 cgcacgagat gatccacccg ccgcgcgtgg acaccgacct cgtgccgaac agcggcccga
     3661 ccgtcgcctc gcgcacggtg atggtggtcg gcgggctgct ggcggaggcg gcgcgcaaga
     3721 tgctggagac gctccgggcc gagggcggtc tgcccgcgcc gcacaccgcc gacgacttcc
     3781 gcgccgcggc ccgccggcgt cacgcggagc acggaccgct cagcgcgacg gcggcgtatc
     3841 gtccacgcga gggctgggcg tgggacgaga aggcgctggt gggcgatgcc tacgccgcgt
     3901 acgcgtgggc gtgctacgtc gccgaggtcg ccgtcgacgt gcggaccggc gaggtggagg
     3961 tgaccgactt cgtcgccgtg caggacgtcg gccgcgtcat caaccccgtc acggcgcgcg
     4021 gtcaggtcca cggcggcgtg acccagggca tcggctttgc gctctacgag gacgtcgtct
     4081 ggcgcgacgg ggtgatggcc aacgcgcgac tgagcgacta catcctgccg acgagcgccg
     4141 acacgccccc gatccgtgcc gtgttcctcg aggtgccgta cgccggcggg cccttcggcg
     4201 ccaaggggct cggcgagctg ccgatggacg gcacggcgcc ggcggtggtg aacgccgtca
     4261 acgccgcgct cggaacggcc ctggcgcgcc tgccggccac gcccgaacac gtgctggagg
     4321 cgtggcaggg gacgccgcac tcatgagagc gcccgagccg ccggcctccc gcggaggcgc
     4381 gctcgcccgc cgacgttgcg gtcggccgtg gccggcgaga tccgcgtgaa cgaccgcatg
     4441 cgggtcgcgc tcaacgtcaa cggcgcggcg cgcgtaatcg aggtgtcacc cgtcgcccgg
     4501 ctgctcgacg tgttgcgcga ggtgctgcgc ctgacgggga ccaaggaagg ctgcggcgag
     4561 ggcgagtgtg gcgcctgctc cgtgctgctc gacggcgcgc tcgtcgtgtc gtgcctggtg
     4621 cccatcggcc agtgcgacgg ggcgtcgatc acgacgatcg agggcctggc gcccgctgga
     4681 gccggacgcc tggcgccggt gcaggaggcg ctgctcgacg agggcggcac ccagtgtggt
     4741 atctgcacgc ccggcatcgt ggtggcggcc caggcgctgc tctcggcgaa ccccacgccg
     4801 gcgctggcgg acattcgtga ggcgctcgcg ggaaacctct gccgctgcac gggctacacg
     4861 gcgatctacg aggccatccg ccgcgcggcc ggcgcccgat gaccgacgcg cccgccgtga
     4921 ccggcggagc gcgcggcgtc gcgcgatgac cgcactgggc tgggtcagcg cgcggacgct
     4981 ggacgaggcg ctcgcccgtc tcgccgatga tcccgcgctc acgccgatcg ccggaggcac
     5041 cgacgtgatg gtggccgccc gcatgggggg gctccgcgag ggccggctgc tcgacctctg
     5101 gcggctcgac gagctgcgcg gcgccgaggt cgcgcgcgac agtctcctgc tcggcgcact
     5161 gaccacgtac acggaaatcg tcacgcaccg ggcgatcgcg acgcgctggc cgctgctggc
     5221 cgccgcggcg cgcgtgagcg gcgcctgggc catccgcaac cgcggcacgc tgggcggcaa
     5281 cgtggccaac gcctcgcccg ccgccgacac gccgcccgcg ttgctggcct acggcgcgcg
     5341 gctcgagctt cgcagcgcgc gcggcgcgcg atgggtggac tatcggagct tccacacggg
     5401 ctatcgccgc acgctgcgcg cgcccgacga gctgatcacg cggatcgccg tgccggcacc
     5461 gcccggggat gccgtccact tctaccgcaa ggtcggcgcg cgtcgcgccc aggcgatcag
     5521 caaggtctgc ttcgcggcga ccgcggcgcg ccgggacggc gcgctcgccg acgtgcgact
     5581 cgccctcgga agcgtggcgc cgacggtggt cagcgcgcgg cgaacggccg catggctcga
     5641 ggggcggcgt cccgccgccg tggaccgcgc cgaggcgcgc gcgctcctcg agtccgagat
     5701 cgcgccgatc gacgacctgc gctcgacggc tgggtaccgc cgccgtgtcg ccggcaacct
     5761 gctcgagcag ttcctggcgg acgccgcgga gcggccctag tgtcgcggat cacgagttcg
     5821 ctggtgaacg cgccggccgg ggcggggtcg ggggcgcggg tccgccgacc cctgatgcgc
     5881 tccgcgtcgt cgctctcgtg ccatttccat cttcggatgg ggcggggttc cgttggcggg
     5941 gaacaggtc
//
LOCUS       DATIAZ010000159         4747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1881954, whole genome shotgun sequence.
ACCESSION   DATIAZ010000159 DATIAZ010000000
VERSION     DATIAZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881954"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..626
                     /locus_tag="VLF19_05545"
     CDS             156..626
                     /locus_tag="VLF19_05545"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSE92749.1"
                     /translation="MIKTGEPAPPFDLADAAGERVALRDLAGRDVVLYFYPKDDTPGC
                     TKEACGFRDLWRELQRAGVVVLGISADDAASHTRFSKKYKLPFTLLSDPDRKVMQAWG
                     AYGDKMMYGKKVKGVIRSTVWVGPDGVVRKHWTRVRDAARHPEEVFEAIRGKAA"
     gene            654..1913
                     /locus_tag="VLF19_05550"
     CDS             654..1913
                     /locus_tag="VLF19_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92750.1"
                     /translation="MVRTTRRTILKGLVGAGSAAAVTGFPFVNRLALGQQPLKLGALY
                     PFTGPLALEAQEMHTASQIAVDDVNAEGGVMGRKVELVTRDTEFKPEVTKRKATELVE
                     VEKSPFLIGALVGFEGIALSEVGCKNEVVVMFFGQNFLTVKGKMCKHQFGNNLTPYQT
                     AAASARFVAEQKMGKKWHMLANNYSWPQMIERAYSIVAKQVGAEWTGVTWAPLGTRDF
                     LPFLPKVAATKSDVVFVTTWAADQVAAAKQVQEFGLARQAHVFHTITELTFAEAAGKG
                     VYENFYSGMPWYWHLKDKYPGAKRYHDRHLALRKRAPSGYAASTYSTVRLLLDTAREA
                     KTLDTMKLTQALEGRKFQYLKGPEYIRPCDHVNIQEVFLCKGKSASAMKTESDYFDIV
                     QAVGGEVVAEACESEGFDPKKPLSQQI"
     gene            1967..2812
                     /locus_tag="VLF19_05555"
     CDS             1967..2812
                     /locus_tag="VLF19_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92751.1"
                     /translation="MNGCILILVALGLSIVFGLMGIINFAHGIFYMLGAYAGWVTVQA
                     TGSFWLALVVAPVSVFVVGVVVERGVLKRIYGTRNANFAGVLVTYGLAIMIPDLMRMI
                     FGRPGKPFAAPKILTGSLFTLGATEVGGYRVFILVVTALLLPLLWLLLNKTNLGMVIR
                     AGTSDNLMVQLLGINVSRVWTVTYGLGAALAAFGGVITAPLVAVAPEMGGLILIECFI
                     VTVVGGMGSFVGAVVGGLLIGEVLALTGMVYDVLADVIIYLAMAVVLLVRPRGLFGQE
                     GMAEE"
     gene            2809..4575
                     /locus_tag="VLF19_05560"
     CDS             2809..4575
                     /locus_tag="VLF19_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HSE92752.1"
                     /translation="MTRGRAGLALAALVLATAPLYADYTPFYMALLTEVLVFGLFALA
                     YDVMLGHAGVLSLGHSAFLGVASYAAGLLLARFAAPVELSLLAAGVAGLVTALLLGVF
                     ALRKRGVYLAMLTLALSQVFYFVVLMWTPVTGGTDGLGNLPVLYLSEPLGLRLSKRPV
                     TRYYVVALIVFASMLVIRQILYSPLGRVMRAVKANERRAEACGYDTNRVRLAAFAISG
                     FFSGVAGGLLTLVLEFVPIENIHWAMSGTVLIMALFGGTGTLLGPFVGSAVFVWMRDF
                     VSKHLEYWEVFVGGVFVLIVLFFPEGIVGTLRRLAGARAAVPVRESDTTVTGPAMAPG
                     TPATASAAGDGAVLESYGLTKEFGGLVAVNAVDFRVRPGELRAVIGPNGAGKTTFFNM
                     LTGVLPPSRGRIVFKGRDITRLPAHAVSRLGIARSYQVTNIFADLSVFENIRIAAQSR
                     VTHYRFWRDAERLVDVRRRAEEILALLALDAKRDLPAAALSHGEQRYLEIGIALATDP
                     DFLLLDEPTAGMSPDETGRTAAFVRKLAGRLTIVLVEHDMEVVMGISDRITVLNYGQV
                     LAEGTPAEIRENPEVRRVYLRD"
BASE COUNT          674 a         1706 c         1598 g          769 t
ORIGIN      
        1 ggggcagctg gcggcatatc ttctggagcc cgcctcggag gacggggtgg tcacctggaa
       61 tctgctcgac gccgagctgg cggcgggcca cgcctaccct atcctacgca gcaggggcgc
      121 ggcggccgcc gccgcaccac ctgggagagc tgaagatgat caagaccggt gaaccggcgc
      181 cgcccttcga cctggccgat gccgccgggg agcgggtcgc cctccgggac ctcgccgggc
      241 gggatgtcgt cctctacttc taccccaagg acgacacgcc gggctgcacc aaggaggcgt
      301 gcggctttcg cgacctctgg cgggagctgc agcgcgccgg cgtcgtcgtc ctcggcatct
      361 ccgcggacga cgccgcctcg cacacccggt tctcgaagaa gtacaagctg cccttcacgc
      421 tgctctccga tcccgaccgc aaggtcatgc aggcgtgggg cgcgtacggc gacaagatga
      481 tgtacggcaa gaaggtgaag ggcgtcatcc gctcgacggt ctgggtgggc cccgacggcg
      541 tcgtgcgcaa gcactggacg agggtccgcg acgcggccag gcaccccgag gaggtcttcg
      601 aggccattcg cggcaaggcc gcctgaccgc cgccaccgat ggagggacag ccaatggtcc
      661 gcacgacgcg acgcacgatc ctcaaaggtc tcgtgggcgc cggcagcgcc gccgccgtca
      721 ccgggtttcc cttcgtcaac cgcctggccc tcgggcagca gccgctcaag ctcggcgccc
      781 tctatccctt cacgggcccg ctggccctcg aggcccagga gatgcacacg gcgtcgcaga
      841 tcgccgtcga cgacgtcaac gccgagggcg gcgtgatggg gcgcaaggtg gagctggtca
      901 cgcgcgacac ggagttcaag cccgaggtca ccaagcgcaa ggcgacggag ctggtcgagg
      961 tcgagaagtc gccgttcctc atcggcgcgc tggtcggctt cgagggcatc gcgctgtcgg
     1021 aggtcggctg caagaacgag gtcgtggtca tgttcttcgg ccagaacttc ctcaccgtca
     1081 agggcaagat gtgcaagcac cagttcggca acaacctgac gccctaccag acggccgcgg
     1141 cctcggcgcg cttcgtcgcc gagcagaaga tgggcaagaa gtggcacatg ctcgccaaca
     1201 actactcgtg gccgcagatg atcgagcgcg cctactcgat cgtggccaaa caggtgggcg
     1261 ccgagtggac gggcgtgacc tgggccccgc tgggcacgcg cgacttcctg ccgttcctgc
     1321 ccaaggtggc ggcgacgaag tccgacgtcg tgttcgtgac aacgtgggcg gccgaccagg
     1381 tggcggcggc caagcaggtg caggagttcg ggctcgcccg gcaggcgcac gtcttccata
     1441 ccatcaccga gctgaccttc gcggaggccg cgggcaaggg cgtgtacgag aacttctact
     1501 cggggatgcc ctggtactgg catctcaagg acaagtaccc gggcgccaag cggtaccacg
     1561 accgccacct cgcgctgcgc aagcgcgccc cctcgggcta cgccgcgtcc acctactcca
     1621 ccgtccggct gctgctcgac accgcccgcg aggccaagac gctcgacacc atgaagctga
     1681 cccaggcgct cgagggccgg aagttccagt acctcaaggg gcccgagtac atccgcccct
     1741 gcgaccacgt caacatccag gaggtcttcc tctgcaaggg caagtcggcc tcggcgatga
     1801 agaccgagtc cgactacttc gacatcgtgc aggccgtcgg cggcgaggtc gtcgccgagg
     1861 cctgcgagag cgagggattc gacccgaaga agccgctgag ccagcagatc tgacgctctt
     1921 cggctatccg ctggagatct ggtacacgca gctcttcatc gggctggtga acggctgcat
     1981 cctgatcctc gtcgccctgg ggctgtcgat cgtgttcggc ctcatgggca tcatcaactt
     2041 cgcgcacggc atcttctaca tgctgggggc ctacgcgggc tgggtcaccg tgcaggccac
     2101 cggcagcttc tggctggccc tcgtcgtggc gccggtgagc gtcttcgtcg tgggcgtcgt
     2161 cgtggagcgc ggagtcctca agcgcatcta cggcacccgg aacgccaact tcgccggcgt
     2221 gctcgtcacc tacggcctgg cgatcatgat ccccgacctc atgcggatga tcttcggccg
     2281 cccgggcaag cccttcgcgg cgccgaagat cctcacgggc tcgctcttca cgctgggggc
     2341 caccgaggtc ggcggctatc gcgtcttcat cctcgtcgtc accgcgctcc tgctgcccct
     2401 cctgtggctc ctcctcaaca agaccaacct gggcatggtg atccgcgccg ggacgagcga
     2461 caacctcatg gtgcagctgc tcggcatcaa cgtctcgcgc gtgtggacgg tcacctacgg
     2521 cctcggcgcc gcgctggcgg cgttcggggg cgtcatcacc gcccccctcg tcgccgtggc
     2581 gccggagatg ggcggcctga tcctcatcga gtgcttcatc gtcaccgtcg tcgggggcat
     2641 gggcagcttc gtgggcgccg tggtgggcgg cctcctcatc ggcgaggtcc tggcgctcac
     2701 gggcatggtc tacgacgtgc tcgccgacgt catcatctac ctggccatgg cggtggtgct
     2761 gctcgtccgg ccgcgcgggc tcttcggcca ggaggggatg gcggaagagt gacgcgcggt
     2821 cgcgccgggc tcgccctcgc cgcgctcgtc ctcgccacgg cgccgctcta cgcggattac
     2881 acgccgttct acatggcgct gctcaccgag gtcctcgtct tcgggctctt cgccctggcc
     2941 tacgacgtca tgctcggtca cgccggcgtg ctctcgctcg gccactcggc gttcctcggg
     3001 gtcgcgtcgt acgccgccgg gctgctcctg gcgcgcttcg ccgcccccgt cgagctgagc
     3061 ctgctggcgg cgggcgtcgc cgggctcgtc accgcgctcc tgctcggcgt cttcgccctg
     3121 cgcaagcgcg gcgtgtacct ggccatgctc acgctcgcgc tctcgcaggt cttctacttc
     3181 gtggtgctca tgtggacgcc ggtcacgggc ggcaccgacg gcctcggcaa cctccccgtg
     3241 ctgtacctga gcgagccgct ggggctccgc ctctcgaagc gccccgtcac gcgctactac
     3301 gtggtggcgc tgatcgtctt cgcctcgatg ctggtcatcc ggcagatcct gtactcgccg
     3361 ctcggacgcg tgatgcgcgc cgtgaaggcc aacgagcgcc gcgccgaggc ctgcggctac
     3421 gacaccaacc gcgtgagact cgcggccttc gccatctcgg ggttcttctc gggcgtggcc
     3481 ggcggcctcc tcaccctcgt cctggagttc gtgccgatcg agaacatcca ctgggcgatg
     3541 tcggggaccg tcctcatcat ggcgctcttc ggcggcaccg gcacgctcct cgggcccttc
     3601 gtgggctcgg ccgtcttcgt gtggatgcgc gacttcgtca gcaagcacct cgagtactgg
     3661 gaggtcttcg tcggcggcgt cttcgtcctc atcgtgctct tcttccccga gggcatcgtc
     3721 gggaccctgc gccggctcgc cggcgcccgc gcggcggtcc ccgttcgcga gtcggacacg
     3781 acggtgaccg ggcccgccat ggcgcccggc acgccggcga ccgcgagcgc ggccggcgac
     3841 ggcgccgttc tcgagagcta cggtctcacg aaggagttcg gcggcctcgt ggccgtcaac
     3901 gccgtggact tccgggtgcg cccgggcgag ctgcgcgcgg tcatcggtcc gaacggtgcc
     3961 ggcaagacga ccttcttcaa catgctgacg ggcgtcctgc cgccctcccg cggccgcatc
     4021 gtcttcaagg gccgcgacat cacccggctg ccagcgcacg cggtgtcgcg cctcggcatc
     4081 gcgcgctcct accaggtgac caacatcttc gccgacctca gcgtcttcga gaacatccgg
     4141 atcgccgcgc agtcgcgcgt gacgcactac cggttctggc gggacgcgga gcggctcgtg
     4201 gacgtgaggc gccgcgccga ggagatcctg gccctcctcg cgctcgacgc caagcgcgac
     4261 ctgcccgccg ccgcgctgtc ccacggcgag cagcgctatc tcgagatcgg catcgcgctg
     4321 gcgaccgacc ccgacttcct gctgctcgac gagcccaccg cgggcatgag ccccgacgag
     4381 accgggcgca ccgcggcgtt cgtccgcaag ctggcgggcc ggctgaccat cgtgctggtg
     4441 gagcacgaca tggaggtcgt catgggcatc tccgaccgca tcaccgtgct gaactacggc
     4501 caggtgctgg ccgagggcac gccggcggag atccgcgaga accccgaggt ccgccgcgtg
     4561 tacctgcgcg attgatcaca aggcggcccc gatccagacc ccgactcagt gacaccagcc
     4621 tcgggggcgt cgctgccgcc cgcgatgatg ccggcgatct cgtcccgggt caacgtcgcc
     4681 gcgagaacgg gccggttgcc gcagcgggcc aggagggcct gccggagggc gcgggcgacc
     4741 tcctctg
//
LOCUS       DATIAZ010000160        16415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_305037, whole genome shotgun sequence.
ACCESSION   DATIAZ010000160 DATIAZ010000000
VERSION     DATIAZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305037"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VLF19_05565"
     rRNA            complement(<1..434)
                     /locus_tag="VLF19_05565"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(720..2009)
                     /gene="tyrS"
                     /locus_tag="VLF19_05570"
     CDS             complement(720..2009)
                     /gene="tyrS"
                     /locus_tag="VLF19_05570"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSE92753.1"
                     /translation="MTSPPLLRDLEARGLVADATHPAELGAHLTAGPRVLYCGFDPTA
                     DSLHVGSLLPLLVLRRFQLAGHRPIVLIGGGTGLIGDPSGKTTERALNPEEQVAAWAA
                     RLKRQVARFVDFDTGPAPARLLDNYEWLSKLTVISFLRDAGKEFGVGAMIAKESIRAR
                     MGRTDVGLSYTEFSYQILQAYDFLTLFRDHGCTLQIGGTDQWGNITAGIDLIRRTLGA
                     AAYGLTQPLVTKADGEKFGKTESGTVWLDPAKTSPYEMFQFWLNTADADVATYLKYFT
                     FLTLDEIEQLAEATRRAPQEREAQRVLAERVTDIVHGQPARREAERITAALFRGDVRS
                     LSEAELEHVTRTLPTAPLPAPVPGVLVPLVDVLTASGLAGSRRAARELITTGAIQVNG
                     DRVRDAAARVSSHDALHGRYLILTRGRKTHAAAKFGG"
     gene            2051..4366
                     /locus_tag="VLF19_05575"
     CDS             2051..4366
                     /locus_tag="VLF19_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HSE92754.1"
                     /translation="MAWGRRSQPEDEEPARPPGLRRWLRRALIGVGVLTLAVGVVAGV
                     AVLWAFTILPRSLPAVSALETFQPVLGTKLYDDNDELLTELHVERRIFVPLGQIPQAL
                     RDAVIATEDRRFYGHWGIDPIGVARAVVQNYRRGRIVEGGSTITQQLTKVLFLTPDKS
                     LERKLKEAVLALELERRYSKDRILEMYLNQVYFGHGAYGVEAAARTYFGKSVSELNVR
                     EAALIAGLPRAPTSYSPFEHPQAAKLRRELVLRRMVEYGSVKDAEAKRLARTDLGLIS
                     PERRRTTGQYFLEHVERTLEAKYGADMVFKGGLNVYTTLNPWMQLAAEQAVREGLKAL
                     ESRTSKGAQEHPEGALVTIDPQTGFVKAMVGGYDFFRSEFNRAVQAKRQPGSAFKPLV
                     YIAALEAGFTPATRVDDAPVTYPAGRNGNAWKPENYDRLFRGPTTLQQAVEESVNVVT
                     VKVQERIGVNRTIQVARRLGITSALNVDLTLALGTSDLTLLELTSAYSALANQGTWLP
                     PTGIRYITDAGGRLLEENVPEGKAAMAPETAYVVTHMLRGVVERGTGQSAKALGRPVA
                     AKTGTTNDYSNAWFIGFTPNLATGVWVGYDRPRSLGKDETGSRVAVPIWTTFMSRVLV
                     GSPKEDFPIPDRVVLVPVDLDPSNECVRVVTMAFVKGTEPAVGCGPRRQQVPGKPPES
                     GPGTPEAPPEGPGAPGAPSPAPNALTPSSRVLPPITGAAPPAAAAPAVGLPVPLVPGG
                     GARPGGGSPSSPPSRGEPIVLPPSAATAQSP"
     gene            complement(4377..5849)
                     /locus_tag="VLF19_05580"
     CDS             complement(4377..5849)
                     /locus_tag="VLF19_05580"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HSE92755.1"
                     /translation="MDVLVSLCKRRGFIFQSSEIYGGTGSCWDYGPLGVELKNNIKRV
                     WWRDFVQQRDDIVGLDASILMHPTVWTASGHVDLFTDPMADCRECKRRFRADHVEAVP
                     WVHYCPATKGNKFEVPGGEPCRHCGARRTLCPECGKGELTPPRQFNLMFKTFMGPVEE
                     DAAVTYLRPETAQAMFVNFDNVLQSMRLKLPFGIAQAGRSFRNEITPGNFIFRTREFE
                     QMEIEFFVNPRETIDGRPADEAWHDRWIQDCLEWFRRYGLQDENLQLREHDKAELAHY
                     AKRTVDIEYRFPMGWSELMGIANRTDFDLKQHSKFSGKSLTYFDEERREHIVPYVVEP
                     AAGVDRALLAFMLDAYREEEVRGERRVVLQLHRELAPLKVAVLPLLKKRGDIVATAQE
                     IRDRLSRRWRVVYDDTAAIGRLYRRQDEVGTPFCVTVDVQTVGDADKGEHGDGAVTIR
                     ERDSMEQVRVPIADLVAVFDRLMGEGVWEDVARGYPRPKP"
     gene            complement(5852..6616)
                     /gene="recO"
                     /locus_tag="VLF19_05585"
     CDS             complement(5852..6616)
                     /gene="recO"
                     /locus_tag="VLF19_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HSE92756.1"
                     /translation="MALGKTAAVVIGGFPLGESDRVVTFFSRQYGKIRGVARAARRIK
                     SRFAGALELGTLGELVFFDTGRSDLVRVDHFDVLRPFARVRDDLGRLGHAAWIVEFVG
                     RLTADRDANPAVYGLLVRALGSVDAGAAAGRVAVTFGLRCVEALGHRLRTDACAACGR
                     RVTLAQGTVAVDVDAGGVLCGACGAGRRDTVALAAGSVLALRRLRGAAWEEATTVRLG
                     AAERELRMLLERQMAALTGHPPRSARFVRTVEELTR"
     gene            complement(6618..7493)
                     /gene="era"
                     /locus_tag="VLF19_05590"
     CDS             complement(6618..7493)
                     /gene="era"
                     /locus_tag="VLF19_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HSE92757.1"
                     /translation="MALIGRPNAGKSTLLNRLVGAKLAIVSPRPQTTRNRITGIRNLP
                     GAQIVFVDTPGLAAPGGKLGEFMTRTVERALEEVDLVCAVIDATERTHSDARLLAVLA
                     AHPAPAFALLNKADAVVPRSRMLPIIDAWRTAHPFREILPVSALDGMNCDRLLDLIVA
                     AMPEHPPLFPEEATSDQPETFWVAEVIREKIFHLTHAEVPYAAAVRVDDLTERRRPPR
                     LDIRASVFVERESQKGILIGRGGAMLKRIGTAARQELEAFFGVQVFLGLTVHVRRAWR
                     KDDRALREFGFRLTS"
     gene            complement(7634..8119)
                     /gene="ybeY"
                     /locus_tag="VLF19_05595"
     CDS             complement(7634..8119)
                     /gene="ybeY"
                     /locus_tag="VLF19_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSE92758.1"
                     /translation="MKPPSSEMSCRVDDRQHRVAVDGPRLARAGDRALGALGRAGGAV
                     EILVVDDHAIRDLNARWRGIRRRTDVLAFPLETPGAPGGLVGQIVISAQTAARQARRV
                     GVTLGDELDLLATHGVLHLVGWDDRDPVEAGLMHEREREILSSGPRRPPPRLWKGLLH
                     P"
     gene            complement(8116..9162)
                     /locus_tag="VLF19_05600"
     CDS             complement(8116..9162)
                     /locus_tag="VLF19_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSE92759.1"
                     /translation="MSEATTVTRRLSIAPDVDLLPLLGRNDDHLRTLESELDVRIVAR
                     GHDIVLKGDERQVRRAERALTQLADLVRSGAPVRAVEVRAAIRMLSDDEDADLKSVFA
                     DAIPVPSRKKWVAPKTLGQKRYVDAIRKHDMVFGIGPAGTGKSFLAVAMAVSALMKRE
                     VARIVLTRPAVEAGERLGFLPGDLYEKVHPYLRPLYDALYDMLEAEKVQTLTEKGAIE
                     IAPLAYMRGRTLNDSFIILDEAQNSTTEQMKMFLTRLGFNSKMVITGDVTQVDLPASR
                     GSGLIEVQGILRDVPGIGFVYFDEHDVVRHRLVSAIIRAYDTHEAAPGSGTRSRAAAL
                     PPQRELGDVETPAD"
     gene            complement(9159..10961)
                     /gene="aspS"
                     /locus_tag="VLF19_05605"
     CDS             complement(9159..10961)
                     /gene="aspS"
                     /locus_tag="VLF19_05605"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HSE92760.1"
                     /translation="MSAYRTHTCGELRRQHVGATVRLSGWVHRKRDHGGLLFIDLRDN
                     YGLTQCVLHSGNPEFAAAEALRLESVVAVTGRVLARSAETVNPKLATGEIETAVDDVT
                     VLSVADPLPIQVNTDTEYPEETRLRHRFLDLRRDKLHRNILLRSQVIASIRRRMIAAG
                     FTEYQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLLMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDLEMSFVTQEDVFAAVEPVLHGLFEEFRPDCSVTPPPFP
                     RIPFDTAMARYGTDKPDLRNPLVVDDATGVFRDSDFAVFATAIRERGAVVRALRAPHG
                     AGQPRSFFDRLNAWAREQGGPGLGYVVLAEGAVRGPIAKFLDGPRLARLRETTGAGDG
                     DAVFFVCDQRAAADRLAGRARVKLGDDLALVEKNAYRFCWVTDFPMYERNEETGAIEF
                     SHNPFSMPQGGLEALETRDPLAIKAFQYDIVCNGVELSSGAIRNHRPDIMFKAFALAG
                     YRREDVEARFGGMLNAFRYGAPPHGGSAPGIDRIVMLLAGETNLREVVAFPMNQQAQD
                     LLMQAPAPVPPERLKELHIRLDLPVVDSRAKPTS"
     gene            complement(10973..12271)
                     /gene="hisS"
                     /locus_tag="VLF19_05610"
     CDS             complement(10973..12271)
                     /gene="hisS"
                     /locus_tag="VLF19_05610"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSE92761.1"
                     /translation="MTARLDTHPPRGMRDLLPDEVEVRDRATATILDVYRRHGFQRIE
                     TPAVESIKLLTRSEGGENEKLIFKILKRGEKLVAGQDVEDLVDLGLRFDLTVPLARYY
                     ANNHGRLPHPLKAIQIGPVWRAERPQAGRFRQFTQCDIDILGVASETAEIELIIATAE
                     ALLALGLTDLTVRLNDRRILAAVARHCGFEEARFDSVFIALDKLDKIEKAGMIGELEA
                     EGHPAKAIGGLLALLDEVTDTAWRLDRLAPLLREHWDPGVGRALERILGEVQHYGRGE
                     FQIRFDPTLVRGMGYYTGPIFEVQYRQARSSIAGGGRYDRMIGRLLGRDVPATGFSIG
                     FERVIGILMEEATPAAHDRERVALVFDEQAATLTPALRLARELREQGSIVSLEIKSKK
                     AGRQLQELELRGFGRIGVVGEDGAVEWRVRRSQPVSGSGT"
     gene            complement(12314..14194)
                     /locus_tag="VLF19_05615"
     CDS             complement(12314..14194)
                     /locus_tag="VLF19_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92762.1"
                     /translation="MMSVVRSRPMPLAALALALTLVSLAVGSVSGSGQPLQAPSAPTP
                     SFKVLADQVAALFPMVQTEVVEAAEARVTLADGRQTGVQPGIELTVVREGRELYHPTT
                     KKLLGRTQQTLGRVVVTEVFDAYSVARLVEGGGAIQPGDRALATAGKVRLTVLPLTTG
                     GRSKIVESATYDLIQELERTGRFQTVFGDQIIGWLAQERIAVEDFMRGQGVRQATERF
                     KISHLLAVHFTTVQGKPFMDVRAFSSALQAPLLQSALFVPPAVRAEPAQQFSSGANPQ
                     VARIERRSLLARLLSGDWEPNTYSAGAASIPIRSIATFPFMVVSMDVAVAPADKVPRL
                     VITDGQRVFVYRINGEKLDAEWTFDKRQRGRILSVQFADLNGDGVLDVVANRQDVRTG
                     MLSYVLTTRGGRAAVLADDLPLLLLAVDEAGDGINKGLWGQSYSPDRFWTVGTAMRYV
                     LNGNDVAATTRPAVHDAFRPTGATFSNIGGKERVLAFVDEHNRLRVALGGQELWRSIT
                     VVGGGLAQGHLQIPMLQTMVDKYFKFEPNPVSVDLDGDGIHEVVVPVNEDEAGRMAIV
                     FRGPAGYRFQVVSSGFEGFVTGIGAIPGEGANPSLVVAVVRRIGLLKQSGETQIVITL
                     AE"
     gene            complement(14273..15757)
                     /locus_tag="VLF19_05620"
     CDS             complement(14273..15757)
                     /locus_tag="VLF19_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92763.1"
                     /translation="MRKVLAVAVSVVAFLGLLAPPVLAQAPAPKVTITGLFDFATTFS
                     QNWSSLDPTNSEDKEWYSRERAVFTVTGEVGATKAVWAIELDFTNGCNGTGTACTGGH
                     AGTTAGFDLDTDIAGAVETKWLYVETPLTGPGSIMPFIPVATRMRAGAQPARGHEYKN
                     GILLGGDFPGIALETTWAPNVKSTLTYIQIAEKLEPVTAPGATEDFAVLASLEWQIFK
                     GLTIKPTYAYANYRGGNTGTGNLGTEPKNGFNVNGTTLETTRHTFGGDVRWTIGGFTL
                     APTFLYQLGTQEIPAAGGTGEVDISSYIFDMIADYRIGPLTIGGRIMYTPGMSAGDSV
                     AAGEDVNYYQSINPAFGYMAGYTQIQTSGIEYNTAFLAGASGLSLRQSPSYDKYGRIF
                     AALVADYAITPAFTVSGTANASWTPEKVDTDGTLSSTGLTSPTGGDDSFLGWELALGF
                     TYRFAPNVALDLNGAYLFAGDALGNAGNDADDVYKVVARVRFTF"
     gene            complement(15941..>16415)
                     /locus_tag="VLF19_05625"
     CDS             complement(15941..>16415)
                     /locus_tag="VLF19_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE92764.1"
                     /translation="TVPVYWNALGWIYLQLGHPEVAYRAFSRAVELDGTWAEAQLNVG
                     VALAEMGRWEEALAAYRKVLNLPGLSVPHATYQNMGVAFYNLKRYREAEEALRFAIGL
                     EPKLAAAYYHLGLVLVATERREDAKGLFRKARDLAPQSPFGEAAVGQLKALGEGG"
BASE COUNT         2453 a         5777 c         5731 g         2454 t
ORIGIN      
        1 aaggccttcg tcccccacgc ggcgtcactc cgtcaggctt tcgcccattg cggaaaattc
       61 cccactgctg cctcccgtag gagtcggggc cgtgtctcag tccccgtgtg gctggtcgtc
      121 ctctcagacc agctacccgt cgtcgccttg gtgagccgtt acctcaccaa ctagctgata
      181 ggccgcgagc ccctccaaga gcggcagctt gcgccacctt tcctccccgg atttcgcaac
      241 ccgaggaacg tatccggtat tagcctccct ttcgagaggg tatcccgaac tcgagggcag
      301 gttacccacg tgttactcac ccgtgcgcca ctagaagcac cgatgtattg ctacactggt
      361 actcctcgtt cgacttgcat gtgttaagta cgccgccagc gttcgctctg agccaggatc
      421 aaactcgcca tgaacagcga tgaacctggt cctcgaagcc cgaacgtttc actgcattgc
      481 agttacccgg tcgggcgcaa ggtactcgtt agaacggatg gtgctcacac ctcttcggtg
      541 agccgatagc atatgcgact ttcaaagagc gctacggtca gcttggaagg tgaccggggc
      601 ttacttcact aagcctggct cacgtctccg tgagcaacga ctgactatac gcacggcacc
      661 ccgcactgtc aagaccttct gcggccaccc ctccccccgg gccggcccgg gcccttcggt
      721 cagccgccga acttcgccgc cgcgtgcgtc ttgcggcccc gggtcaggat caggtatcgg
      781 ccgtggaggg cgtcgtggga ggagacacgg gcagccgcgt ccctgacccg atcgccgttc
      841 acctggatcg cgccggtggt gatcagctca cgcgccgccc gcctggaccc tgcgaggccc
      901 gaggccgtga gcacgtccac cagcggcaca aggacgccgg gcaccggcgc gggaaggggc
      961 gcggtgggaa gggtccgggt gacgtgctcg agctcagcct cgctgagcga tctgacgtcg
     1021 ccgcggaaga gggccgcggt gatccgctcg gcctctcggc gggccggctg gccgtggacg
     1081 atatccgtca cccgttcggc cagcacccgc tgcgcctcgc gctcctgggg cgcccggcgc
     1141 gtcgcctccg cgagctgctc gatctcgtcg agcgtcagga aggtgaagta cttgaggtac
     1201 gtcgccacgt cggcgtccgc cgtgttcagc cagaactgga acatctcgta gggcgaggtc
     1261 ttcgccggat cgagccacac ggtgccggat tccgtcttgc cgaacttctc gccgtccgcc
     1321 ttggtcacga gcggctgggt gaggccgtag gccgcggccc cgagcgtgcg gcggatgagg
     1381 tcgatgccgg ccgtgatgtt gccccactgg tcggtgccgc cgatctgcag cgtgcagccg
     1441 tgatcgcgga agagtgtcag gaagtcgtac gcctggagaa tctggtagct gaactcggtg
     1501 taggagagcc ccacgtcggt ccgccccatg cgcgcccgga ttgactcttt cgcgatcatc
     1561 gcgccgacgc cgaactcctt gcccgcgtcg cggaggaacg agatcaccgt cagcttcgag
     1621 agccactcgt agttgtcgag cagccgcgcc ggggcgggcc cggtgtcgaa gtccacgaac
     1681 cgcgcgacct ggcgcttgag cctcgcggcc cacgcggcga cctgctcttc ggggttgagc
     1741 gcgcgctcgg tggtcttgcc gctcgggtcc ccgatgagcc cggtcccgcc gccgatcagc
     1801 acgatgggac ggtggccggc gagctggaag cggcgcagca cgagcagggg caggaggctg
     1861 cccacgtgca gactgtcggc ggtggggtcg aagccgcagt agagcacgcg cggcccggcg
     1921 gtcaggtgcg cgccgagctc ggcgggatgc gtcgcgtccg caacgagacc gcgggcttcg
     1981 aggtccctca ggagcggggg cgatgtcacg tgcggaagca tatcgtatcg cccgttataa
     2041 tgtggggacc atggcgtggg gccgacgctc gcagcccgag gacgaggagc cggctcgccc
     2101 gcccggcctc cgtcgctggc ttcgacgcgc gctgatcggt gtcggtgtcc tcactctggc
     2161 cgtgggcgtg gtcgccggcg tcgccgtgct ctgggccttc acgatcctgc cccgctcgct
     2221 gccggccgtg agcgcgctcg agaccttcca gccggtgctc ggcaccaagc tctacgacga
     2281 caacgacgag ctcctcaccg agctgcacgt cgagcggcgc atcttcgtgc cactggggca
     2341 gatcccccag gcgctgcgcg acgccgtcat cgccacggag gaccgccgtt tctatggcca
     2401 ctgggggatc gacccgatcg gcgtcgcccg ggccgtcgtg cagaactacc ggcgcgggcg
     2461 catcgtcgag ggtggcagca cgatcacgca gcagctcacc aaggtgctgt tcctgacgcc
     2521 cgacaagagc ctcgagcgga agctcaagga ggccgtcctc gcgctggagc tggagcgccg
     2581 ctactccaag gaccgcatcc tggagatgta cctgaaccag gtgtacttcg gccacggcgc
     2641 ctacggcgtc gaggcggccg ccaggaccta cttcggcaag tccgtgtccg agctgaacgt
     2701 ccgggaggcg gcgctgatcg ccggcctgcc ccgcgccccc acgagctact cccccttcga
     2761 gcacccgcag gccgcgaagc ttcgccgcga gctggtgctg cgccggatgg tcgagtacgg
     2821 gtccgtcaag gacgccgagg ccaagcgcct ggcgaggacc gacctcggcc tcatctcgcc
     2881 cgagcgccgg cgcacgaccg ggcagtactt cctcgagcac gtcgagcgga cgctggaggc
     2941 gaagtacggc gccgacatgg tcttcaaggg cgggctcaac gtgtacacca cgctgaaccc
     3001 ctggatgcag ctggccgccg agcaggccgt ccgcgagggc ctgaaggcgc tcgagagccg
     3061 gacgagcaag ggcgcgcagg agcaccccga gggcgcgctg gtgacgatcg atccgcagac
     3121 cgggttcgtc aaggcgatgg tcggaggcta cgacttcttc cgcagcgagt tcaaccgtgc
     3181 ggtgcaggcc aagcgccagc cgggctcagc cttcaagccg ctcgtgtaca tcgccgccct
     3241 ggaggccgga ttcacgccgg ccacgcgcgt cgatgacgcg ccggtgacct atcccgccgg
     3301 ccgcaacggc aacgcctgga agccggagaa ctacgaccgc ctgttccgcg gccctaccac
     3361 gctgcaacag gcggtcgaag aatcggtgaa cgtggtcacc gtgaaggtgc aggagcgcat
     3421 cggcgtgaac cgcaccatcc aggtcgcccg gaggctcggc atcacgagcg cactgaacgt
     3481 cgacctcacg ctcgcgctcg gcacctccga cctcaccctg ctcgagctca cctccgccta
     3541 cagcgcgctg gccaaccagg gcacctggct gcccccgacg gggatccgct acatcaccga
     3601 cgccgggggc aggctcctcg aggagaacgt ccccgagggc aaggcggcga tggcgccgga
     3661 gaccgcgtac gtcgtgaccc acatgctacg cggcgtggtc gagcgcggca ccggtcagag
     3721 cgccaaggcg ctcgggcggc ctgtcgccgc gaagacgggg accaccaacg actactcgaa
     3781 cgcctggttc atcggcttca cgccgaacct cgcgacgggt gtgtgggtcg gctacgatcg
     3841 cccgcgcagc ctcggcaagg acgagaccgg ctcgcgcgtc gccgtcccga tctggacgac
     3901 gttcatgagc cgggtgctgg tcggctcgcc caaggaggac ttcccgatcc cggaccgcgt
     3961 cgtgctggtg ccggtggacc tcgatccctc gaacgagtgc gtccgcgtcg tgacgatggc
     4021 cttcgtgaag ggcacggagc ccgctgtcgg ctgcggtccg cgccggcaac aggttccggg
     4081 caagcctccg gagtccgggc ctggcacgcc ggaggcgcca ccggagggac cgggggcgcc
     4141 gggggcgccg tcccccgccc cgaacgccct gacgccgtcc tctcgggtgc tacccccgat
     4201 cacgggcgcg gcgccgccgg ccgcggcagc gccagccgtg gggctgccgg tcccgctggt
     4261 gccgggtggt ggggcacggc cagggggcgg ctcgccctcc tcgccaccgt cgcgcggcga
     4321 gccgatcgtg ctgccgccga gcgcggcgac ggcgcagtcg ccctgagcga ccgcgctcag
     4381 ggcttggggc gcgggtagcc gcgcgcgacg tcctcccaca ccccctcgcc catcagccgg
     4441 tcgaacacgg cgacgagatc ggcgatcggg acgcggacct gctccatcga gtcacgctcg
     4501 cggatggtca cggcgccgtc accgtgctcg cccttgtcgg cgtccccgac cgtctggacg
     4561 tcgaccgtca cgcagaacgg tgtgccgacc tcgtcctggc gccggtagag gcggccgatg
     4621 gcggccgtgt cgtcgtagac gacccgccac cggcgggaca gccggtcccg gatctcctgc
     4681 gcagtggcga cgatgtcgcc ccgcttcttg agcagcggca gcacggcgac cttgagcggg
     4741 gcgagctcgc ggtgcagctg cagcaccacc cgcctctcgc cacggacctc ctcctcgcgg
     4801 taggcgtcca gcatgaaggc cagcagcgcg cggtccacgc cggcggccgg ctccaccacg
     4861 tagggcacga tgtgctctcg tcgttcctcg tcaaagtacg tgagcgactt cccgctgaac
     4921 ttggaatgct gcttgaggtc gaagtccgtg cggttggcga tccccatcag ctcgctccag
     4981 cccatcggga accggtactc gatgtccacg gtgcgcttgg cgtagtgggc gagctccgcc
     5041 ttgtcgtgct cgcggagctg gagattctcg tcctgcaggc cgtaccggcg gaaccactcc
     5101 aggcagtcct ggatccagcg gtcgtgccag gcctcgtcgg cgggccggcc gtcgatcgtc
     5161 tcccgcgggt tgacgaagaa ctcgatctcc atctgctcga actcgcgcgt gcggaatatg
     5221 aagttgcccg gcgtgatctc gttccggaag gaccggcccg cctgcgcgat gccgaagggc
     5281 agcttgagcc gcatcgactg cagcacgttg tcgaagttga cgaacatcgc ctgcgcggtc
     5341 tccgggcgca gataggtgac ggccgcgtcc tcctcgaccg ggcccatgaa ggtcttgaac
     5401 atcaggttga actgccgcgg cggggtcagc tcgcccttcc cgcactccgg acacagcgtg
     5461 cggcgcgcgc cgcagtgccg gcacggctcg ccccccggga cctcgaactt gttgcccttg
     5521 gtggccggac agtagtggac ccacggcacg gcttcgacgt ggtcggcccg gaaccgccgc
     5581 ttgcactccc ggcagtcggc catggggtcg gtgaagaggt cgacgtggcc gctcgccgtc
     5641 cagacggtcg ggtgcatgag gatcgaggcg tcgagaccga cgatgtcgtc gcgctgctgg
     5701 acgaaatcac gccaccacac gcgcttgatg ttgttcttca gttcgacgcc gaggggccca
     5761 taatcccagc aggagccggt gccaccatag atctcgctcg actggaagat gaaaccccgt
     5821 cgcttgcaca gcgacacgag cacgtccatg gtcatcgcgt caactcctcc accgtccgca
     5881 cgaagcgcgc gctgcggggc ggatggcccg tgagggcggc catctggcgc tcgagcagca
     5941 tgcgcagctc gcgctcggct gcacccagcc gcaccgtcgt cgcctcctcc cacgccgccc
     6001 cgcgcaggcg ccgcagcgcc agcacgctgc cggcggcgag cgccaccgtg tcgcggcgcc
     6061 cggcgccgca cgcgccgcac agcacgcccc cggcgtcgac gtccacggcg accgttccct
     6121 gcgcgagggt cacccgccgg ccgcacgccg cgcaggcgtc cgtgcggagc cggtggccca
     6181 gcgcctcgac gcagcggaga ccgaacgtca ccgccacgcg cccggcggcg gcgccggcgt
     6241 ccacgctgcc cagcgcgcgc accaggagcc cgtagaccgc ggggttggcg tcgcggtcgg
     6301 cggtgagccg ccccacgaac tcaacgatcc atgccgcgtg gcccagccgt ccgagatcgt
     6361 cgcgcacgcg cgcgaacggc cggagcacgt cgaagtggtc cacgcggacc aggtcgctgc
     6421 gccccgtgtc gaagaacacc agctcgccca gcgtgcccag ctcgagggcg cccgcgaacc
     6481 gcgacttgat ccggcgcgcg gcccgggcga cgccgcggat cttgccgtac tgccgggaga
     6541 agaacgtgac gacccggtcg ctctctccga gcgggaaccc cccgatcacg accgccgccg
     6601 tcttgccgag ggccatgtca ggacgtcagc cggaacccga actcgcgcag cgcgcgatcg
     6661 tccttccgcc acgcccggcg cacgtggacg gtcagcccga gaaagacctg gacgccgaag
     6721 aacgcctcca gctcctgccg tgcggccgtc ccgatgcgtt tgagcatcgc accgccgcga
     6781 ccgatcagga tgcccttctg cgactcgcgc tcgacgaaca cgctcgcccg gatgtcgagc
     6841 cggggtggcc ggcggcgctc cgtgaggtcg tccacgcgca cggccgctgc atacgggacc
     6901 tccgcgtggg tcaggtggaa gatcttctca cggatgacct cggcgaccca gaaggtctcg
     6961 ggctggtcgc tggtggcctc ctcgggaaag agcggcggat gctcgggcat cgcggcgacg
     7021 atcaggtcga ggaggcgatc gcagttcatg ccgtcgagcg cggaaaccgg gaggatctcg
     7081 cggaagggat gcgccgtgcg ccaggcgtcg atgatcggca gcatccggct ccggggcacc
     7141 acggcgtcgg ccttgttcag cagggcgaag gccggcgcgg ggtgcgcggc gagcacggcg
     7201 agcagacgcg cgtcggagtg agtccgctcg gtcgcgtcga tcacggcgca caccaggtcc
     7261 acctcctcaa gggcccgctc caccgtccgc gtcatgaact cgccgagctt gccgcccggc
     7321 gcggcgagcc cgggcgtgtc cacgaagacg atctgcgcgc cgggcaggtt gcggatgccg
     7381 gtgatgcggt tgcgcgtcgt ctgcggcctg ggcgacacga tcgcgagctt ggcgccgacc
     7441 aggcggttca gcagcgtgga cttgccggcg ttcggccggc cgatcagcgc cacgaacccc
     7501 gcacgagccc cggagggggg ctccgctctc cttccggaag cttcccccga ggccgattgc
     7561 gctggcgcag ccggtgctcg agcgtccacc aggatccgtt ccgcgggcga ggcccgcgct
     7621 cgagggcgcc gggtcatggg tggaggaggc ccttccagag gcggggcggg ggccgtcggg
     7681 ggccggagga gagaatctcg cgctcgcgct cgtgcatgag gccggcttcg acggggtcgc
     7741 ggtcgtccca gcccacgagg tgcagcacgc cgtgggtggc gaggaggtcc agctcgtcgc
     7801 cgagggtcac gcccacccgg cgcgcctgcc gggcggccgt ctgcgcggag atcacgatct
     7861 gcccgacaag gccgccgggc gctccgggag tctcgagggg aaacgccagg acgtcggtgc
     7921 ggcgccggat accgcgccac cgggcattca gatccctgat ggcgtggtcg tccacgacca
     7981 ggatctcgac ggcgcccccg gcacgcccca gggcgccgag cgcccggtcg cccgcgcggg
     8041 ccaggcgcgg gccgtccacg gcgacgcggt gctggcggtc atcgacccgg cacgacatct
     8101 cgctcgaagg aggcttcaat cggctggggt ctcgacgtcc cccagctccc gctgcggcgg
     8161 caacgccgcc gctcgagagc gcgtcccgct gcccggggcg gcttcgtggg tgtcgtaggc
     8221 gcggatgatc gcggacacga ggcggtggcg gacgacgtcg tgctcgtcga agtagacgaa
     8281 gccgatgccg ggcacgtccc ggaggatgcc ctgcacctcg atcagccccg agccccgcga
     8341 ggccggcagg tccacctggg tcacgtcgcc ggtgatcacc atcttggagt tgaagcccag
     8401 gcgcgtgagg aacatcttca tctgctccgt cgtgctgttc tgcgcctcgt cgaggatgat
     8461 gaacgagtca ttcagcgtcc gcccccgcat gtacgcgagc ggggcgatct cgatggcgcc
     8521 cttctcggtg agcgtctgta ccttctcggc ctcgagcatg tcgtagagcg cgtcgtagag
     8581 cggccgcagg tacggatgca ccttctcgta aagatcgccg ggcaggaaac ccaggcgctc
     8641 gccggcctcc acggccggcc gggtcagcac gatgcgggcg acctcgcgct tcatgagcgc
     8701 ggacacggcc atggccacgg cgaggaacga cttgccggta ccggcgggcc cgatgccgaa
     8761 gaccatgtcg tgcttccgga tggcgtccac gtagcgcttc tggcccaggg tcttgggggc
     8821 gacccacttc ttgcgggacg gcaccgggat ggcgtcggcg aataccgact tgaggtcggc
     8881 gtcctcgtcg tcgctcagca tgcggatcgc ggcccgcacc tcgacggcgc ggacgggggc
     8941 gcccgagcgc acgaggtcgg cgagctgcgt gagcgcgcgc tcggcgcggc ggacctgccg
     9001 ctcgtcgccc ttcagcacga tgtcgtggcc ccgggcgacg atgcgcacgt ccagctcgct
     9061 ctcgagggtc cggagatgat cgtcgttccg cccgagcagc ggaaggagat ccacgtcggg
     9121 ggcgatcgag aggcgtcggg tgacggtggt ggcttcgctc aactcgtcgg cttcgccctc
     9181 gagtctacca cgggcagatc gagtcggatg tggagctcct tgagccgctc gggcggcacc
     9241 ggcgccggcg cctgcatcag gaggtcctgc gcctgctggt tcatggggaa ggcgacgacc
     9301 tcccggaggt tggtctcacc cgcgagcagc atgacgatgc ggtcgatgcc gggcgccgag
     9361 cctccgtgcg gcggcgcgcc ataccggaag gcgttcagca tgccgccgaa gcgcgcctcg
     9421 acgtcctcgc gacggtagcc ggcgagggcg aaggccttga acatgatgtc gggccggtgg
     9481 ttgcgaatgg cgccgctcga cagctccacg ccgttgcaga cgatgtcgta ctgaaaggcc
     9541 ttgatggcca gcgggtcccg cgtctccagg gcctcgagac caccctgggg catagagaac
     9601 gggttgtggc tgaactcgat ggcgcccgtc tcctcgttgc gctcgtacat ggggaagtcg
     9661 gtcacccagc agaagcggta cgcgttcttc tccaccagcg cgaggtcgtc gccgagcttc
     9721 acgcgcgccc ggcccgccag ccggtcggcg gcggcgcgct gatcgcacac gaagaacacg
     9781 gcgtcgccgt ccccggcgcc ggtcgtctcg cggagccgtg ccagccgcgg cccgtcgagg
     9841 aacttggcga tggggccccg gacggcgccc tcggccagca cgacgtagcc gagccccggc
     9901 ccaccctgct cgcgcgccca cgcgttcagc cgatcgaaga agctgcgggg ctgcccggcg
     9961 ccgtggggcg cgcgcagcgc ccgcacgacc gcgccccgct cgcggatcgc cgtcgcgaac
    10021 accgcgaagt cggagtcccg gaacaccccg gtcgcgtcgt cgacgacgag cggattgcgg
    10081 aggtccggct tgtccgtgcc gtaccgcgcc atcgccgtgt cgaacgggat gcgcgggaac
    10141 ggcggcggcg tgaccgagca gtcgggccgg aactcctcga agaggccgtg cagcacgggc
    10201 tcgacggccg cgaagacgtc ctcctgggtc acgaaggaca tctcgaggtc gagctggtag
    10261 aactcgcccg gcgagcggtc ggcccgcgcg tcctcgtcgc ggaagcaggg ggcgatctgg
    10321 aagtagcggt cgaagcccgc gaccatcagg agttgcttga actgctgggg cgcctgcggc
    10381 agcgcgtaga acttgccggg atgcacgcgg ctgggaacga ggtagtcgcg ggcgccctcg
    10441 ggcgagctcg ccgtcaggat cggcgtctgg tactcggtga agcccgccgc gatcatgcgc
    10501 cgccggatgg acgcgatgac ctggctgcgc agcaggatgt tgcggtggag cttgtcgcgc
    10561 cggaggtcca ggaagcggtg gcggagccgc gtctcctcgg gatactcggt gtcggtgttc
    10621 acctggatcg gcagcgggtc ggcgaccgac agcacggtga cgtcgtcgac ggcggtctcg
    10681 atctcgccgg tggcgagctt cgggttcacc gtctcggcgc tcctggccag tacccggccc
    10741 gtcacggcga cgacgctctc gaggcgcagc gcctcggccg cggcgaactc cgggttgccc
    10801 gaatggagca cgcactgggt cagcccgtag ttgtctcgca ggtcgatgaa caggaggccg
    10861 ccgtggtcgc gcttgcgatg gacccagccc gacaggcgga ccgtggcgcc gacgtgctgg
    10921 cgacgcagct ccccgcaggt gtgggtccgg tacgcggaga tcaggggcga cgtcacgtcc
    10981 cgctgccgct caccggctgg ctccgccgga ctcgccactc gaccgccccg tcctcgccca
    11041 cgactccgat gcggccgaac ccgcggagct ccagctcctg gagctgccgg cccgccttct
    11101 tgctcttgat ctccagcgac acgatgctcc cctgctccct cagctcccgc gccaggcgca
    11161 gggcgggcgt cagcgtggcc gcctgctcgt cgaacaccag cgccacgcgc tcgcggtcgt
    11221 gcgccgccgg cgtcgcctcc tccatgagga tgccgatcac gcgctcgaag ccgatcgaga
    11281 agccggtggc cgggacgtcg cggcccagca gcctgccgat catccgatcg taccgcccgc
    11341 cgccggcgat ggacgagcgc gcctgccggt actgcacctc gaagatcggc ccggtgtagt
    11401 accccatgcc gcgcacgagg gtgggatcga acctgatctg gaactcgccg cgcccgtagt
    11461 gctggacctc gccgaggatc cgctccagcg cacggccgac gccggggtcc cagtgctcgc
    11521 ggaggagcgg cgcgagccga tcgaggcgcc acgcggtgtc cgtgacctcg tcgagcagcg
    11581 ccagcagccc gccgatcgcc ttcgccggat gtccctccgc ctccagttcc ccgatcatgc
    11641 cggccttctc gatcttgtcg agcttgtcca gcgcgatgaa caccgagtcg aacctggcct
    11701 cctcgaaccc gcagtgacgc gcgaccgcgg cgaggatccg gcggtcgttg aggcggacgg
    11761 tcagatcggt gagcccgagg gcgagcagcg cctcggcagt ggcgatgatg agctcgatct
    11821 cggccgtctc ggacgccacg ccgaggatgt cgatatcgca ctgggtgaac tgccggaacc
    11881 ggccggcctg ggggcgctcc gcgcgccaga ccgggccgat ctggatcgcc ttgagcggat
    11941 gcgggagccg cccgtggttg ttcgcgtagt agcgggccag gggcacggtg aggtcgaacc
    12001 gcagcccgag atcgacgagg tcctcgacgt cctgccccgc gaccagcttc tctccccgct
    12061 tgaggatctt gaagatcagc ttctcgttct cgcccccctc gctgcgggtc aggagcttga
    12121 tgctctccac ggcgggggtc tcgatccgct ggaagccgtg ccggcggtag acgtccagga
    12181 tcgtcgcggt ggcgcggtcg cggacctcga cctcgtcggg cagcaggtcg cgcatgccgc
    12241 gaggggggtg ggtgtcgagc cgggcggtca ccggcgaggc tccgtggagt cgatcacgag
    12301 gcgaggtcgg cccctactcg gcgagcgtga tcacgatctg cgtctcgccc gactgcttca
    12361 acaggccgat ccggcggacc acggccacga ccagcgaggg attggcgccc tcgccgggga
    12421 tcgcgccgat cccggtcacg aagccctcga agcccgagct gaccacctgg aagcggtagc
    12481 cggcggggcc gcggaagacg atggccatcc gcccggcctc gtcctcgttg acgggcacca
    12541 cgacctcgtg gatgccgtcg ccgtcgaggt cgaccgagac cgggttcggc tcgaacttga
    12601 agtacttgtc gaccatcgtc tgcagcatcg gaatctgcag gtggccctgt gcgaggccgc
    12661 cgcccaccac cgtgatcgac cgccacagct cctgcccgcc gagcgccacc cgcaggcggt
    12721 tgtgctcgtc gacgaaggcg agcacccgct ccttgccgcc gatgttcgag aaggtggccc
    12781 cggtcggccg gaaggcgtcg tgcaccgccg ggcgggtggt ggccgcgacg tcgttgccgt
    12841 tgagcacgta gcgcatcgcc gtcccgacgg tccagaaccg gtcggggctg tacgactggc
    12901 cccagaggcc cttgttgatg ccgtcgccgg cctcgtccac ggcgaggagc agcagcggaa
    12961 gatcgtcggc cagcacggcc gcacgcccgc cgcgcgtcgt gagcacgtag gacagcatcc
    13021 ccgtgcgcac gtcctgtcgg ttggcgacga cgtccaggac gccgtcgccg ttgaggtcgg
    13081 cgaactgcac gctcaggatc cggccccgct gccgcttgtc gaaggtccac tcggcgtcga
    13141 gcttctcgcc gttgatccgg tagacgaaga cgcgctggcc atcggtgatg accaggcgcg
    13201 gcaccttgtc ggcgggggcc accgccacgt ccatcgagac caccatgaac gggaacgtgg
    13261 cgatggaccg gatggggatc gacgctgcgc ccgccgagta cgtgttcggc tcccagtccc
    13321 cggacagcag gcgcgcgagc agcgaccgcc gctcgatgcg cgcgacctgg gggttcgccc
    13381 ccgacgagaa ctgctgcgcc ggctccgccc gcaccgcggg gggcacgaag agcgcgctct
    13441 gcaacagagg cgcctgcagc gcggacgaga aggcgcgcac gtccatgaag ggcttgccct
    13501 gcacggtcgt gaagtgcacg gccagcaggt gcgagatctt gaagcgctcg gtcgcctgcc
    13561 gcaccccctg cccgcgcatg aagtcctcga cggcgatgcg ctcctgcgcg agccagccga
    13621 tgatctgatc gccgaacacg gtctggaacc ggccggtccg ctccagctcc tggatcaggt
    13681 cgtaggtcgc ggactccacg atcttggagc gccccccggt cgtcagcggc agcaccgtga
    13741 gccgcacctt gccggccgtc gccagcgccc ggtccccggg ctggatggcg cccccaccct
    13801 cgacgagccg ggcgaccgag tacgcgtcga acacctcggt gaccacgacc cgccccaggg
    13861 tctgctgtgt gcgcccgagg agcttcttcg tggtcgggtg gtacagctcc cggccctccc
    13921 gcacgacggt cagctcgatg cccggctgca cgcccgtctg ccgcccgtcg gccagggtca
    13981 cccgggcctc ggcggcctcc acgacctccg tctgcaccat cggaaatagc gcagccacct
    14041 gatcggcgag aaccttgaaa gacggcgtcg gcgccgacgg cgcctgcaag ggctgtccgc
    14101 tgcccgacac ggagccgacg gcgagcgaca ccagcgtcag cgcgagggcg agggccgcga
    14161 gcggcatggg gcggctgcgc accacggaca tcaccgggac tatatcaccc ctggaaaacg
    14221 aggagggcat gggggagacc ccatgccctc cgtgctgtcc ccgtggagcc gattagaacg
    14281 tgaaccggac gcgagcgacc accttgtaga cgtcgtcggc gtcgttccct gcgttcccca
    14341 gcgcgtcacc cgcgaacagg taggcgccgt tcaggtcgag cgcgacgttc ggagcgaagc
    14401 gatacgtgaa gccgagcgcc agctcccacc cgaggaacga gtcgtcaccg cccgtggggc
    14461 tcgtcaagcc cgtggaggac agggtgccgt cggtgtccac cttctcgggc gtccagctgg
    14521 cgttggcggt acccgacacc gtgaacgccg gcgtgatcgc gtagtcggcc acgagggcgg
    14581 cgaagatgcg gccgtacttg tcgtacgacg gcgactgccg gaggctgaga cccgaggcgc
    14641 cggccaggaa ggccgtgttg tactcgatgc cggacgtctg gatctgcgtg tagccggcca
    14701 tgtacccgaa ggccgggttg atcgactggt agtagttgac gtcctcaccc gcggccacgc
    14761 tgtcgccggc gctcatgccc ggcgtgtaca tgatccggcc gccgatggtc aggggaccga
    14821 tgcggtagtc ggcgatcatg tcgaagatgt aggagctgat gtcgacctcg cccgtcccgc
    14881 cggccgccgg gatctcctgc gtgccgagct ggtagaggaa cgtcggcgcc agcgtgaagc
    14941 cgccgatggt ccagcggacg tccccgccga acgtgtggcg ggtggtctcc agcgtggtgc
    15001 cgttcacgtt gaagccgttc ttgggctcgg taccgaggtt gcccgtgccc gtgttgccac
    15061 cgcggtagtt ggcataggcg taggtgggct tgatcgtcag gcccttgaag atctgccact
    15121 cgaggctcgc caggacggca aaatcctcgg tggcgccggg cgcggtcacc ggctcgagct
    15181 tctcggcgat ctggatgtag gtcagggtcg atttgacgtt cggcgcccag gtggtctcca
    15241 gggcgatccc ggggaagtca ccgccgagca ggatgccgtt cttgtactcg tggccgcggg
    15301 ccggctgggc gccggcgcgc atccgggtcg ccacggggat gaagggcatg atcgacccgg
    15361 gaccggtgag aggcgtttcg acgtacagcc acttcgtctc gacggcgccc gcgatgtcgg
    15421 tgtccaggtc gaatccggcc gtcgtgccgg catggccacc ggtgcaggcc gttccggtgc
    15481 cgttacaacc gttggtgaag tccagctcga tggcccacac ggccttggtg gcacccacct
    15541 caccggtgac cgtgaacacc gcgcgctcgc gcgagtacca ctccttgtcc tccgagttcg
    15601 tcgggtcgag cgatgaccag ttctgcgaga aggtagtggc gaaatcgaac aacccggtga
    15661 tcgtcacctt cggggctggc gcctgggcga gaacgggtgg tgccagcaac ccgaggaagg
    15721 cgaccaccga cactgcgacg gcgaggactt tcctcattac cgcctcctca tgtggtggtg
    15781 cgtcgttcgg ataccacatc acgctcagtc caacaccaac acctgcagag actcctgggg
    15841 acagcgggac ccttgtagcc ccggcgtcgg cagcaggtca acaaaaaacg gcagaatcgg
    15901 cagatcggcc acccatgcca ccacccctag tgcatgcgcg tcatcccccc tcccccaggg
    15961 ccttgagctg tcccaccgcc gcctccccga acggggactg gggggcaagg tcgcgggcct
    16021 ttctgaacaa gcccttagcg tcctcccggc gctcggtggc gaccaggacg aggccgagat
    16081 ggtagtaggc ggccgccagc ttgggctcca gcccgatggc gaacctcagg gcttcctcgg
    16141 cctcgcggta gcgcttgagg ttgtagaagg cgactcccat gttctggtag gtggcgtgcg
    16201 gcaccgacag gccggggagg ttgaggacct tccggtacgc cgccagcgcc tcctcccatc
    16261 gccccatctc ggccagcgcc acccccacgt tgagctgggc ctcggcccac gtgccgtcca
    16321 gctcgacggc ccgggagaac gcacggtagg ccacctcggg gtggccgagc tgcaggtaga
    16381 tccacccgag cgcgttccag tagacgggca ccgtc
//
LOCUS       DATIAZ010000161         4127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1488619, whole genome shotgun sequence.
ACCESSION   DATIAZ010000161 DATIAZ010000000
VERSION     DATIAZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488619"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..976
                     /locus_tag="VLF19_05630"
     CDS             440..976
                     /locus_tag="VLF19_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSE92765.1"
                     /translation="MSDQRLAVAEVAALLRAAGAALRAEAGALPERVLRHHPAPGEWC
                     VKEVIGHLIEAERRGFAGRIRHILAADRPPLQAWDQDAVAHERRDCERAAGDLLAELA
                     TLRDDSVALVAGLQAADLSRGGQHPRVGFLTVGDLVQEWVHHDRNHLRQMLENVRDFV
                     WPAMGAAQRFSGGAATRP"
     gene            988..2448
                     /locus_tag="VLF19_05635"
     CDS             988..2448
                     /locus_tag="VLF19_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSE92766.1"
                     /translation="MPLALALRDASFHVREVRLRLPFRFGVITVTEATLLHLAITAEA
                     AGGRRARGFAGDFLAPKWFDKDPTKSHRRNVEDLIESARDARAAYADAARAPRAFWPI
                     WRDAYAECRRRGAARGFNGLVVSFGSALFERALADAAGRLAGADVVEMLQQDLLGLRP
                     GEIHSGLAGRRALAWADDGPPETVAVRHTVGLADPITAADLPPDGGPRDGLPRTLEEY
                     VARHGLRYFKLKVAGQVEEDLDRIARVAAVLDRSIPDRYVITLDGNEQYKALEPFAEL
                     LVLLDREPRLARLASAVVFVEQPLDRVIALDPAATGGLTALARRWPMLIDESDGELDS
                     FRMAIALGYRGVSSKNCKGIVKAFLNRTLVERLNAERAPGERLFMSAEDLTNVPVVPL
                     QQDLATVRALGLTHVERNGHHYVRGLAHCSPAERGAATRRHADLYDGNEREAALRIRD
                     GRLALGSLAVPGYGVAVDPDLDSMTPVEAWLARTRS"
     gene            2445..3461
                     /locus_tag="VLF19_05640"
     CDS             2445..3461
                     /locus_tag="VLF19_05640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92767.1"
                     /translation="MSGALRLGLALSGQHPPGDPQAARFAEHVEQVRLARVVGFESVW
                     AGQHYMAAPFSYFQPIPTLARVAAEAEGMRLGTGVILLPLHRPVDIAEQIATLDVITG
                     GRVVFGAGLGYRDDENLAMGIDPRERVGRLEEGLAVLERLWSGEAVSFEGRHFRLTGV
                     RISMPTLQRPRPPIWLAANADGGIRRAARLADAWLMNPHATDATLERQLALFRETRRA
                     LGRPAPLEVPLIKECCLAADRATAVATACPFLEEKYRAYRQWEQDKALPPGESLALDF
                     EVLARDRFLVGDPERVADDIARLREKLGITTLIVRAQWPGMAQDAVLRTIRLLGERVL
                     SRLG"
     gene            3681..>4127
                     /locus_tag="VLF19_05645"
     CDS             3681..>4127
                     /locus_tag="VLF19_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92768.1"
                     /translation="MSTVGRRTFLKTAAGAAAAGFPLPLRAQPKTFRIGVIHPVTGPL
                     AEPGQACRIGAQMAADAVNAAGGIKALGGVKLELLLGDTTTKPDVGRTEAERVISQGA
                     QMLMGSFDSGSTAAMVPVVQQRRVPFLVDIAAADPITANVAKAVKEG"
BASE COUNT          533 a         1532 c         1501 g          561 t
ORIGIN      
        1 gacacgtatg ggaggacagt cgggctccgg cagctcccac gcgagcaggg ccggaatcca
       61 gtatcgacgc atggtgatgc ccatcggcgt gccggggtcg acccgggtca acagctcgtt
      121 gtcttcgcgt gagagcatgg ccacggcctc ctgacgcgtc gcgagggacg tcgcgcgccc
      181 gtccaggaag gtacgccaga gcggccgaaa aggcgagggg cggacacggg gctccgggcg
      241 gagccgggtg gacctgcgca tcgacgatca gcatgatgcg cctcctggag tgggggagcc
      301 tttctgggtc gggatcagat aggagcggga tcgtcgcgcg gggccgcggt ggccgggcct
      361 ccccggtcgg cggcattccg ccgcgccccg ccgtgaaata tactcacggg atgcgggagg
      421 tcgttccggt gtcggctcgg tgagcgacca gcgcctcgcg gtcgctgaag tggccgcgct
      481 cctgcgcgcc gccggggcag cgctccgcgc cgaggccggc gcgctgccgg agcgcgtgct
      541 gcgccaccac ccggcgccgg gagaatggtg cgtgaaggag gtgatcggcc acctcatcga
      601 ggccgagcgc cgtggcttcg ccgggcgcat ccgccacatc ctcgcggccg atcgaccgcc
      661 gctgcaggcc tgggaccagg acgcggtcgc gcacgagcgg cgtgactgcg agcgcgcggc
      721 cggtgacctg ctcgcggagc tcgcgacgct gcgcgacgac agcgtcgccc tcgtggccgg
      781 gctgcaggcc gccgacctct cacgcggcgg ccagcacccg agggtaggct tcctcaccgt
      841 cggcgatctc gtccaggagt gggtgcatca cgaccgcaac cacctgcggc agatgctcga
      901 gaacgtccgt gacttcgtgt ggccggccat gggtgccgcg cagcggttct ccggcggcgc
      961 cgcgactcgc ccctgaacgc aggcgacatg ccgctcgccc tcgcgctccg ggacgcctcg
     1021 ttccacgtcc gcgaggtgcg tctgcggctg cccttccgct tcggcgtgat cacggtgacc
     1081 gaggcgacgc tgctgcacct cgcgatcacc gccgaggccg ccggcggccg ccgggcccgc
     1141 ggcttcgccg gcgacttcct cgcgccgaag tggttcgaca aggacccgac gaagtcccat
     1201 cgccggaacg tcgaggacct gatcgagagc gcacgcgacg cgcgcgcggc gtacgccgac
     1261 gccgcccgcg cgccgcgcgc cttctggccg atctggcgcg acgcctacgc cgagtgccgc
     1321 cggcgcggag cggcgcgcgg gttcaacggg ctcgtggtct cgttcggctc ggccctgttc
     1381 gagcgggcgc tggccgacgc cgccgggcgc ctggccggcg ccgacgttgt ggaaatgctg
     1441 cagcaggacc tcctgggcct ccggcccggc gagatccatt ccgggctcgc cggccgccgc
     1501 gcgctcgcct gggccgacga cggcccgcca gagactgtcg ccgtgcgcca cacggtgggc
     1561 ctcgcggacc ccatcaccgc cgccgacctc ccgccggacg gcgggccgcg cgacggcctg
     1621 ccgcgcacgc tggaggagta cgttgcgcgc cacgggctcc gctacttcaa gctcaaggtc
     1681 gccgggcagg tcgaggagga tctcgaccgg atcgcccgcg tcgccgcggt cctggaccgc
     1741 tcgatccccg accgctacgt catcacgctc gacggcaacg agcagtacaa ggccctcgag
     1801 cccttcgccg agttgctcgt cctcctggat cgcgagccgc ggctcgcgcg cctggcgtct
     1861 gccgtcgtgt tcgtggagca gccgctcgac cgggtcatcg cgctcgatcc cgccgcgacg
     1921 ggcggtctga cggcgctggc gcggcgctgg cccatgctga tcgacgagag cgacggtgag
     1981 ctggactcgt tcaggatggc gatcgcgctg ggctaccggg gcgtgtcgtc gaagaactgt
     2041 aaagggatcg tcaaggcctt cctcaaccgg acgctcgtcg agcgcctgaa cgccgagcga
     2101 gcgccgggtg agcggctctt catgagcgcc gaggacctca ccaacgtccc cgtcgtgccc
     2161 ctgcagcagg acctcgccac cgtgcgcgcg ctcgggctca cccacgtcga gcgcaacggc
     2221 caccattacg tccgcgggct cgcccactgc tcgccggccg agcgcggcgc cgccacgcgc
     2281 cggcacgccg atctctacga tggcaacgag cgcgaggcgg cgctcaggat ccgcgacgga
     2341 cggctcgcgc tcggctcgct cgccgtgccc ggctacggcg tggccgtcga ccccgacctc
     2401 gactcgatga cgccggtcga ggcctggctc gcacggacgc gctcatgagc ggggcgctgc
     2461 gcctcgggct cgcgctctcg ggccagcatc cgcccggcga cccccaggcg gcgcgcttcg
     2521 ccgagcacgt ggagcaggtg cggctggccc gggtcgtggg cttcgagtct gtatgggcgg
     2581 gccagcacta catggcggcg ccgttcagct acttccagcc catccccacg ctcgcgcgcg
     2641 tggccgccga ggcggagggc atgcggctgg gcaccggggt catcctgctg ccgctccacc
     2701 gccccgtgga catcgccgag cagatcgcca cgctcgacgt catcaccggg gggcgggtcg
     2761 tcttcggcgc gggcctcggc taccgggacg acgagaacct cgccatgggg atcgacccgc
     2821 gcgagcgcgt cgggcggctg gaggagggac tcgcggtcct cgagcggctc tggtccgggg
     2881 aggcggtgag cttcgagggt cgccacttca ggctgacggg cgtgcggatc tcgatgccga
     2941 cgctccagcg cccgcgcccg ccgatctggc tcgccgccaa cgccgacgga ggcatccgcc
     3001 gggccgcgcg cctggcggac gcctggctca tgaacccgca cgccaccgac gccacgctcg
     3061 agcgccagct cgcgctcttc cgcgagacgc gccgagcgct cggccgcccc gcgccgctcg
     3121 aggtgccgct gatcaaggag tgctgcctgg cggctgaccg cgccacggcc gtcgccaccg
     3181 cgtgcccgtt cctcgaggag aagtaccggg cctaccgcca gtgggagcag gacaaggcgc
     3241 tgccgcccgg cgagagcctc gcgctcgatt tcgaggtgct cgcccgtgac cggttcctcg
     3301 tcggtgatcc cgagcgtgtc gcggacgaca tcgcgcggct ccgcgagaag ctcgggatca
     3361 ccacgctcat cgtccgcgcg cagtggccgg gcatggccca ggacgccgtg ctccggacga
     3421 tccgcctgct cggggagcgc gtcctgtcgc ggcttgggtg agcgcgcccc catcccgcga
     3481 ccatggcgtc ggcgcacgtc tccgtggtgg cgctcgtcgc gggggcttcg gccgaccggc
     3541 ccgctcgagc gacggcgggt cgaccagccg tgggcgcgtg atcgcccggc tgcgcccgtc
     3601 ctcgtgaagg tgctcgtggt tgggcgccac ggcgcgtcac ctcgtataat tccgccacaa
     3661 ccaggccagg aggcttgtcg atgagcaccg tcggtcgccg cacgttcctc aagaccgccg
     3721 ccggcgccgc cgccgccggc ttcccgttgc cgctccgcgc gcagccgaag accttcagga
     3781 tcggcgtcat ccatcccgtg acgggcccgc tcgccgagcc tggtcaggcc tgccggatcg
     3841 gcgcgcagat ggcggccgac gccgtcaacg ccgcgggagg catcaaggcg ctcggtggcg
     3901 tgaagctcga gctgctgctg ggcgatacga cgaccaagcc cgacgtcggg cgcaccgagg
     3961 cggagcgcgt gatcagccag ggcgcccaga tgctgatggg ctcgttcgac tcggggagca
     4021 cggcggccat ggtgcccgtc gtccagcagc gccgcgtgcc attcctcgtg gacatcgccg
     4081 cggcggaccc gatcaccgcc aacgtcgcca aggccgtgaa ggaaggg
//
LOCUS       DATIAZ010000162         4609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_569566, whole genome shotgun sequence.
ACCESSION   DATIAZ010000162 DATIAZ010000000
VERSION     DATIAZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569566"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VLF19_05650"
     CDS             <1..285
                     /locus_tag="VLF19_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSE92769.1"
                     /translation="VAGFWAKLYVFWAAAAAGLYWLVLVGAILTVVALFYYLIVARRM
                     YIDAPAASGCVPVPVTLAAALILCLLGVVGLGVWPKPVVMAALRVAAPLF"
     gene            complement(439..1167)
                     /locus_tag="VLF19_05655"
     CDS             complement(439..1167)
                     /locus_tag="VLF19_05655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSE92770.1"
                     /translation="MTVAPDVTIILPVYNEVENLGPLWAELEPALGRLGRKAEILVVD
                     DGSTDGSSAFVRGLARGDERVRLVRLGQNAGLTAALLAGFAAARGEIVVTMDSDLQYH
                     PDDIVPLVGLTDRFDAALGYRGRREDPWLKRISSRIANRVRTEVLQDEVRDSACTLRA
                     MHRDCLRAIAPYHGMHRFVPTLLKQAGFRVTEVEVRHRPRRHGESKYGVRNRTFRAFA
                     DLLAVRWMRSRRVVYEVVEDTARL"
     gene            complement(1164..2744)
                     /locus_tag="VLF19_05660"
     CDS             complement(1164..2744)
                     /locus_tag="VLF19_05660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSE92771.1"
                     /translation="MSLRHPRLALVVVLALAAVVFAAPVGRKPFWSSDEARFAVLAQD
                     ILDHGRWLVPELRGDLYLNKPQLYFWSIALVSLPGGRVTELTAAVPSVVSALATLGAV
                     AVTGRLLWGWPVGVLAALVLATTPAFFIFSHAVLSDVMMTAWMTWALYFLVRAARDGW
                     TLGPVLAFHACIGAAVLSKGPAGLAMLVAAGIAIVTTFGVRTLPRLRPGTALAVYAVF
                     ALAWVVPYMTRSRGRFVSSVLVGHYGPWYLQGGLEPRLSQVGSLLGNFLPWTIALAAA
                     VAWWWRDRDEGRRLVGVATLGLLAVLALSGTPRARYLLPVYPGLALLVAEFLCRAAAG
                     GARSAVRIASWTFVALLVVFATAGPFLLPGVKGDDKAYVPEGTGELAVMMTLLAAAAL
                     VNGVAAWRGAYAKGAAAAAVLFLGVLLVEGVCYPPRYARDNDLRPLAEAATRHTGPAG
                     VVVAYPEMRLSFDFYTPRAVVVADTQERVREAAAAATPSAMITTVGHWAEIAPLLPSW
                     RVVDRRQVADRDYVVTAP"
     gene            complement(2741..4393)
                     /locus_tag="VLF19_05665"
     CDS             complement(2741..4393)
                     /locus_tag="VLF19_05665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSE92772.1"
                     /translation="MPADSVLPASIPAGGADRRRRLGGALLVCAVAALLILPAVGRQY
                     ITSSDEARFVLIARDMLQRGVWFDAVVREKHYRNKPPLYPWSIAALARLGAGVTETTA
                     GLPVALAAVTAALCTFLLGEALFGLRAGIAAGLILTTSYSFFQHSLEVLPDMLVVAFA
                     TVAGWAFWRSASTQTPRRRGWLVLLWAAVAMGCFSKGPVGLLPLLPAGVWLWTEGGPA
                     ALRRLWSLPGTAVFVLITLVWVAPFVGLGASSFASNVVVGNWLSWYLGVPSLDTLGNA
                     LLDGVVGFLPWAVPAVLAFPLAWGTRRQPAVRFALLSTIVPLVVVLLAENQRRRYLLP
                     VYPGAALIVAWWATSRLEASPLARRAVAWLGLGAAAAGIAAHAVLGRSPDWFVGVWSW
                     PSMPIYVALAGLGAALFVGLHQARPDVLVGGTATAMLLLLGWAVHPYSAWVNRTQDFP
                     QLAARLERHARGGDAAVYGGRYFPLDFYLGRPLVRLRDAQQLNEYLARPDAPPVVLDN
                     RAWHALQDQIARPVVELDVVRVRAWDMRVVRRADAAPAGTPR"
BASE COUNT          760 a         1636 c         1692 g          521 t
ORIGIN      
        1 gtcgccgggt tctgggccaa gctgtacgtg ttctgggcgg cggccgccgc cggcctctac
       61 tggctcgtcc tggtcggcgc gatcctgacc gtggtggcgc tcttctacta tctgatcgtc
      121 gccagacgga tgtacatcga cgcaccggcc gcgtccgggt gcgtgcccgt gccggtgacg
      181 ctcgccgccg cgctgatcct ctgcctcctc ggcgtcgtcg ggctgggggt gtggccgaag
      241 ccggtggtga tggccgcgct gcgggtggcc gcgccattgt tctagtcggg ggagcggtgc
      301 cgctcccccc gacaccccac cgggatcgcg cgagttgggg aatggggatt gcgcgggcga
      361 agcccgcgcg aatcgcgcgt tcaccagtgg ctgttcgccg accggcgcat caggtcacgg
      421 cggttggact ctccagcgct atagccgggc ggtgtcctcg acgacctcgt agacgacgcg
      481 gcgggagcgc atccaccgga cggcgaggag gtcggcgaaa gcgcggaacg tgcggttgcg
      541 cacgccgtac ttcgactcgc cgtggcggcg agggcggtgg cggacctcca cttcggtcac
      601 gcggaagccg gcctgcttga gcagcgtagg aacgaagcgg tgcatgccgt gatagggggc
      661 aatcgccctc agacagtcac ggtgcatcgc gcgcagcgtg caggcgctat cccggacctc
      721 gtcctgcagc acctccgtcc ggacgcggtt ggcgatgcgc gacgagatcc gcttcaacca
      781 ggggtcttct cggcgcccgc gatagcccag ggcggcgtcg aagcggtccg tgagaccgac
      841 gagcgggacg atgtcgtccg ggtggtactg gaggtccgag tccatcgtca ccacgatctc
      901 gccgcgcgcg gcggcgaagc cggccagcag cgcggccgtg agcccggcgt tctgccccag
      961 tcgcaccagg cgcacgcgct cgtcgccgcg cgccagcccg cggacgaacg cggagctgcc
     1021 gtcggtcgag ccgtcgtcga ccacgaggat ttccgccttg cggccaagcc ggccgagcgc
     1081 cggctcgagc tcggcccaca gggggcccag gttctccacc tcgttgtaga ccggcaggat
     1141 gatcgtgacg tcgggcgcga ccgtcacggc gccgtcacca cgtagtcgcg gtccgcgacc
     1201 tgccggcgat cgacgacacg ccacgacggc agcagcggag cgatctcggc ccagtggccc
     1261 acggtcgtga tcatcgcgct cggtgtcgcg gccgccgccg cctcccggac gcgctcctgg
     1321 gtgtccgcga ccacgactgc tcgcggcgtg tagaagtcga acgacagtcg catctccggg
     1381 taggccacga ccacgcccgc cggtcccgtg tggcgagtcg ccgcctccgc cagcggccgg
     1441 aggtcgttgt cccgcgcgta gcggggcggg tagcacacgc cctcgaccag caggacgccg
     1501 aggaacagca cggcggcggc agccgccccc tttgcgtacg cgccgcgcca tgcggcgaca
     1561 ccgttgacga gcgcggccgc cgcgagcagc gtcatcatga ctgccagctc gcccgtgccc
     1621 tcgggcacgt aggccttgtc gtcgcccttc acgcccggca gcaggaatgg accggcggtg
     1681 gcgaagacca ccaggagggc gacgaacgtc cacgaggcga tccgcacggc gctgcgggct
     1741 ccaccggccg ccgcccggca gaggaactcc gccacgagga gcgccaggcc cggatagacc
     1801 ggcagcaggt accgcgctcg cggcgtcccc gagagggcga ggacggcgag gaggccgagc
     1861 gtggcgacgc cgaccaggcg ccggccctcg tcgcggtcgc gccaccacca ggcgacggcg
     1921 gccgccagcg cgatggtcca gggcaggaag ttgcccagga gcgagccgac ctgggagagc
     1981 cgcggctcga gcccgccctg caggtaccag ggcccatagt ggccgacgag cacgctcgag
     2041 acgaagcggc cgcgcgaccg ggtcatgtag ggaacgaccc aggcgagcgc gaagaccgca
     2101 tacacggcca gcgccgtgcc ggggcgcagc cgtggcaggg ttcgcacgcc gaaggtcgtc
     2161 acgatggcga tcccggcggc caccagcatg gccaggccgg cggggccctt cgacaggacc
     2221 gcggcgccga tgcaggcgtg gaacgcgagg acgggcccga gcgtccagcc gtcgcgcgcg
     2281 gcgcgaacga ggaaatagag cgcccaggtc atccacgcgg tcatcatcac gtcggacagc
     2341 accgcgtgac tgaagatgaa gaacgccggc gtggtggcga gcacgagcgc cgccagcacg
     2401 ccgaccggcc agccccacag caggcggccg gtcacggcga cggcgccgag cgtggcgagc
     2461 gcggagacga ccgacgggac ggccgcggtg agctccgtca cgcggccacc cggcagcgag
     2521 acgagcgcga tcgaccagaa gtagagctgc ggcttgttga gatacagatc gccccgcaat
     2581 tccgggacga gccaccgccc gtggtccagg atgtcctgcg ccagcacggc gaagcgcgcc
     2641 tcgtcggagg accagaacgg cttgcgcccc acgggcgcgg cgaagaccac ggccgcgagg
     2701 gcgaggacga ccacgagcgc caggcgcgga tgtcgcaggc tcatcgcggc gtcccggcgg
     2761 gcgccgcatc ggcgcgccgc acgacccgca tgtcccaggc gcgtacgcgc acgacgtcca
     2821 gctcgacgac gggccgcgcg atctggtctt gcagcgcgtg ccacgcccgg ttgtcgagga
     2881 cgaccggagg agcgtcgggc cgcgcgaggt actcgttgag ctgctgcgcg tcgcgcagcc
     2941 gcacgagggg gcggccgagg tagaagtcga gcgggaagta gcggccgccg tagacggccg
     3001 cgtcgcctcc ccgcgcgtgc cgctcgagcc gcgcagcgag ctgcgggaaa tcctgggtgc
     3061 ggttgaccca cgccgagtag ggatggacgg cccagccgag cagcaagagc atcgccgtcg
     3121 ccgttccgcc cacgagcaca tcggggcgcg cctggtgcag gcccacgaag agcgcggccc
     3181 ccaggcctgc gagcgccacg tagatcggca tggacggcca ggaccacacg ccgacgaacc
     3241 agtccggcga gcgcccgagc acggcgtgcg cggcgatgcc ggcggcggcg gcgccgaggc
     3301 cgagccaggc gacggcgcgc cgggccagcg gcgacgcctc gagccgcgag gtggcccacc
     3361 atgccacgat cagggctgcc cccgggtaca cgggcagcag gtagcgacgc cgctggttct
     3421 cggccagcag caccacgacg agcgggacga tcgtcgacag cagtgcgaac cggacggccg
     3481 gctggcgtcg cgtgccccag gccaggggaa aggcgagcac ggccgggacc gcccatggca
     3541 ggaaccccac gacgccgtcc agcaacgcgt tgccgagggt gtccagcgac ggcacgccca
     3601 ggtaccacga cagccagttc ccgacgacga cattgctggc gaaggacgac gcaccgaggc
     3661 cgacgaaggg cgccacccag acgagggtga tcagcacgaa gacggccgtg ccgggcaggc
     3721 tccacagccg tctgagcgcc gcaggacctc cctcggtcca gagccacacc ccggcgggca
     3781 gcagcgggag caggccgacc ggccccttgg agaagcagcc catcgcgacc gcggcccaga
     3841 gaagcacgag ccagccccgc cgccggggtg tctgggtcga ggccgagcgc cagaaggccc
     3901 agcccgcgac cgtcgcgaac gcgaccacga gcatgtcggg caggacctcc aggctgtgct
     3961 ggaagaagct gtaggacgtc gtgaggatga gaccggccgc gatgccggcc cgaagcccga
     4021 agagcgcctc gccgaggaga aaggtgcaca gggcggccgt cacggcggcc agggcgaccg
     4081 gcaaccccgc ggtcgtctcg gtgacgcctg cgccgaggcg cgcgagcgcg gcgatgctcc
     4141 aggggtacag cggaggcttg ttccggtagt gcttctcgcg cacgaccgcg tcgaaccaca
     4201 ccccgcgctg gagcatgtcc cgcgcgatca gcacgaagcg agcctcgtca gagctcgtga
     4261 tgtactgccg gcccacggcc ggcagtatca gcaaggcggc aacggcgcac accagcaggg
     4321 cgcccccgag ccggcggcgc cggtcggcgc ctcccgcagg gatcgatgcc gggaggacgg
     4381 aatcggcagg catccgacgg cgattatagc cgcctaaacc cctgatgtga cagtcgtaat
     4441 ttctccgtct tgacagccac ggggtctgct gcctatcctc tgtaccgact ggtcggtaca
     4501 atgacgccaa caacgacggc cggcacacgc tcgagagagg gcaagcaaac ccgcgaggtg
     4561 gtcatcgcgg ccgccaagca cctcatgctg atcaagggct atcacgcga
//
LOCUS       DATIAZ010000163         2300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_306043, whole genome shotgun sequence.
ACCESSION   DATIAZ010000163 DATIAZ010000000
VERSION     DATIAZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306043"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VLF19_05670"
     CDS             complement(<1..690)
                     /locus_tag="VLF19_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSE92773.1"
                     /translation="MTRPRVLIVDDEPDMVENMARILGRAGHDCLTAPDGARAVAALE
                     ADRPDLVVTDLKMPGIDGMEVLRRAHAADPDLPVIVVTGFATIESAVAAVKEGAFDYL
                     PKNFSVDQLQVAVDRALRHRGLARENRNLREQLHQTLGFENIIGRSPVMAHVFELVRK
                     AARVEANILVLGESGTGKELIARAIHANSPRAARAFVPVDCASLPEQLLESELFGHEK
                     GAFTGAVRGKPG"
     gene            complement(687..>2300)
                     /locus_tag="VLF19_05675"
     CDS             complement(687..>2300)
                     /locus_tag="VLF19_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE92774.1"
                     /translation="VARRVGGPRVLALPIIRAMAVVAGCVWVALSPRAHAGLDAADAT
                     MLAFLLYSAALMALLWVWPGATLRLNFLVLLVDLAFALLLIHLTGGAQSTLYLALLLI
                     AGLQSYYYGIRRGVGVAAASSLAYTAVVWPTLSATEAADLAIRLAVLIGTSIGLGLLA
                     DVEEAERIKVLELSSQAHDREQFIRSVVDGLHEGVLALDATGRIVAWNRALEQRYGIK
                     AAEVVGQDLFESLPVALREAWSESVHRLLRGEIDGFTLEHVAHETRNKGHVVQNLKGG
                     LLRQHGRPAGAVILVEDITERVALERAARQSEKLAGLGTLAAGLAHELNNPIGIISSR
                     IELMLLEAAPGTLSDSMREDLTVLHRHAQRVARIVQGLLSFARLAPGDRAAVNLNRIV
                     DDTLLLLEKQVTRDGIRVTRSLAAALPLIHGDPTALQQVLMNLVVNARAAMPDGGEIT
                     VATEPAPGRPDQVRLIVRDTGAGIPAEILPKIFDPFFTTKPSGTGLGLSISYGIVRDH
                     EGTVDVDSRPGQGTTFVLTFPVHPVEAAT"
BASE COUNT          337 a          795 c          850 g          318 t
ORIGIN      
        1 cccgggcttg ccgcggacgg cgccggtgaa ggcgcccttc tcgtggccga agagctcgga
       61 ctcgagcagc tgctccggca gcgaggcgca gtccacgggc acgaacgcgc gcgccgcgcg
      121 ggggctgttg gcgtggatcg cacgggcgat cagctccttg ccggtgccgg actcgccgag
      181 gaccaggatg ttggcctcca cccgcgcggc cttgcggacc agctcgaaca cgtgcgccat
      241 caccgggctc cgcccgatga tgttctcgaa ccccagcgtc tggtggagct gctcgcgcag
      301 gttgcggttc tcgcgcgcga gcccccggtg ccggagcgcg cgatccaccg cgacctggag
      361 ctgatcgacg gagaagttct tcggcaggta gtcgaacgcg ccctccttga ccgcggccac
      421 cgcggactcg atcgtggcga acccggtgac cacgatgacc ggcaggtcgg gatcggcggc
      481 gtgcgcgcgg cgcagcacct ccatgccgtc gatgccgggc atcttgagat cggtgacgac
      541 gaggtcgggg cggtcggcct cgagcgcggc caccgcccgg gcgccgtcgg gggcggtgag
      601 gcagtcgtga ccggcgcggc cgaggatcct ggccatgttc tccaccatgt cgggctcgtc
      661 gtcgacgatg aggacgcgcg ggcgggtcac gtggcggcct ccaccgggtg gacgggaaag
      721 gtcaggacga aggtcgtgcc ctggcccggc cgcgagtcga cgtccaccgt gccctcgtgg
      781 tcccgcacga tcccgtacga gatcgacagc ccgagcccgg tgccgctcgg cttggtcgtg
      841 aagaaggggt cgaagatctt gggcaggatc tcggccggaa tgccggcgcc ggtgtcgcgg
      901 acgatcagcc gcacctggtc cggccggccg ggggccggct cggtggcgac cgtgatctcg
      961 ccgccgtccg gcatggcggc gcgcgcgttg accacgaggt tcatgagcac ctgctggagc
     1021 gcggtcgggt caccgtggat cagcgggagc gccgcggcga gcgatcgcgt cacccggatg
     1081 ccgtccctgg tgacctgctt ctcgagcagc agcagcgtgt cgtccacgat gcggttgagg
     1141 ttcacggccg cgcggtcccc tggcgcgagg cgggcgaagg acagcaggcc ctgcacgatc
     1201 cgcgcaacgc gctgcgcgtg ccggtgaagc acggtgaggt cctcgcgcat cgagtccgac
     1261 agcgttcccg gcgcggcctc cagcagcatc aactcgatcc gggaggatat gatgccgatc
     1321 ggattgttga gctcgtgcgc gagcccggcc gccagcgtgc ccaggccggc cagcttctcg
     1381 gactgccggg ccgcccgctc gagggccacg cgctcggtga tgtcctcgac caggatgacg
     1441 gcgccggctg ggcgcccgtg ctggcgcagc agcccgccct tgaggttctg cacgacgtgc
     1501 cccttgttcc gcgtctcgtg cgcgacgtgc tccagcgtga acccgtcgat ctcgccacgg
     1561 aggaggcggt gaaccgactc gctccacgcc tcgcgcaggg cgaccgggag gctctcgaag
     1621 aggtcttggc ccacgacctc ggccgccttg atgccgtagc gctgctcgag ggcccggttc
     1681 cacgcgacga tccggcccgt cgcgtcgagc gcgaggacgc cctcgtgcag gccgtcgacg
     1741 acgctgcgga tgaactgctc gcgatcgtgc gcctggctgg acagctccag caccttgatc
     1801 cgctcggcct cctcgacgtc ggcgagcagc ccgaggccga tcgaggtgcc gatgaggacc
     1861 gcgagcctga tggcgaggtc ggccgcttcc gtcgcggaca gcgtcggcca gacgacggcg
     1921 gtataggcga gggacgacgc ggccgccact ccgacgccgc gacggatgcc gtagtaatac
     1981 gactgcagcc cggcgatgag caggagtgcc aggtacagcg tgctttgcgc gccgccggtg
     2041 aggtggatga gcaggagcgc aaaagccagg tccaccagca ggacgaggaa attcagtcgc
     2101 agggtcgcac cgggccagac ccacagcagc gccatgagcg ccgcgctgta gaggaggaac
     2161 gccagcatgg tggcgtccgc ggcgtcgagg ccggcatggg ctctcggcga cagggcgacc
     2221 cagacgcagc cggccaccac tgccatcgcc cggatgatcg gcaacgccag cacgcggggg
     2281 ccacccacgc gccgggcgac
//
LOCUS       DATIAZ010000164        10863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_438328, whole genome shotgun sequence.
ACCESSION   DATIAZ010000164 DATIAZ010000000
VERSION     DATIAZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10863
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438328"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /gene="gspM"
                     /locus_tag="VLF19_05680"
     CDS             complement(<1..243)
                     /gene="gspM"
                     /locus_tag="VLF19_05680"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HSE92775.1"
                     /translation="MIGNLSRRERVLISLGVLAAVVVLGWEGVVQPLRERHRAAAELV
                     PAREEVLARRQQLVARKATIATDLETTNARVGELNER"
     gene            complement(240..1913)
                     /locus_tag="VLF19_05685"
     CDS             complement(240..1913)
                     /locus_tag="VLF19_05685"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HSE92776.1"
                     /translation="MRFNPTRFAILALGDRLVAAVIQRRRVETFVVDAETPAAALRAE
                     LDQRRLGVRAVWIGLPRSAVTVKPIDLPAVAGEADDMVRFELERHLPYGGGEDTPFDF
                     VPLASDGDGGAQRVLIAAADRRLVESTLRVAEDAKLRPVSLTVAAHNLLALVARPRRG
                     HVVWVHRTGTGADLLFIDAGALVLSRSVADADDDLVAEEIRRSFTVARWRECDAIWVS
                     GDRETETAGPLHALGAPVGEPPYTAAARRLLAGVSPDGSPGLAELAVAVAAGPRTRPL
                     ELLPRALRPRRLTRTQLVTAGLAALCVILGIVALLVPGWREGRRLAAVNARIAALDPE
                     VRAVEKVMQDLERKRRMLGTVQSIESASVRPLPVLRELTDLLPSDAWLTMLSLDGKGI
                     ELTGQAGAASALIPLLENSSHLERVEFSSPVTRGRDREQFRIRAAWEGGAGSVALAPA
                     SPGRSMPGAPGAQPRATPGAITSGPPPPVAPGVTPPTAPVPPAALTPAPAPPALAPAP
                     TIGVAPTPPVPEGPRPPAGVQPVPSPTLPDGTPAVQPPRSVPLPPGAPR"
     gene            complement(1951..2940)
                     /locus_tag="VLF19_05690"
     CDS             complement(1951..2940)
                     /locus_tag="VLF19_05690"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92777.1"
                     /translation="MREARPREERGFALLAVLLVVALLGMVGAEFAYSMRLEASAARA
                     WRDGIAATHLAEAAVEQAIREIVAAAAAPVVVGMDEAGVLTFYTRERTARPRLSREKV
                     PLGAGHFTYRITDEAARLNVNTAPPDRLDRLLQQLGLDKSERGVIVDSVTDWRDPNEE
                     HRVNGAESDDYYLKLPVPYRSRNANLESVTELLQIRGVTPALFRGSEGKPGLAEFVSA
                     KGRGQVNINTASEQVLRAHGLSDAEVSLIVQARRDEPYFQMPGQFGGRGFTVNTETFR
                     IEAEGLVDDRVQARVTAIVQRRAAGATGGGGATGAGGATGGAAVAYLEWWAGR"
     gene            complement(2940..3557)
                     /locus_tag="VLF19_05695"
     CDS             complement(2940..3557)
                     /locus_tag="VLF19_05695"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HSE92778.1"
                     /translation="MPGRGARGFTLLELMIALGIVGALLVIAFGGLRVALAAWTRGED
                     RAEVHQHLRGVTMVLSRSVGAAYPYRASAGDAPDPVLLFRGSADRLELVTRAVPFPAA
                     APVAFTALVLSIERGEEGEELVVRQRPLPNRNPFTEAEVVLRDPSIQTLALHYLDAAG
                     SWQEEWDAEEQGGLPSAVRIALAIRRGDRTEAQPPLTVSLRTVTP"
     gene            complement(3557..4156)
                     /locus_tag="VLF19_05700"
     CDS             complement(3557..4156)
                     /locus_tag="VLF19_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSE92779.1"
                     /translation="MRRRREEGFTLIEVLVALAILGIAVVAAIQGFAQGLRLLKLSGD
                     HQQAMLVADLKTREVVTPEEMQDEGAEGEFRWRRTTRLLETPDLTPDDGATPRFRVWE
                     IAVSVRWDERRQIEVTTLRTAAVTSTPEATSPGQVPTPGTPPAGSASAPGTLTGSGTP
                     TTPGARSAPGGTATRRPGTTSPRTPTFPGNRPATTPGGR"
     gene            complement(4153..4599)
                     /locus_tag="VLF19_05705"
     CDS             complement(4153..4599)
                     /locus_tag="VLF19_05705"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HSE92780.1"
                     /translation="MGKRQDLDARGFTLLELVIVLMVLGLAVALVAPAVGRSTATIRT
                     RTEVARFAALLRHAREQAITTRSAHDLIVEPRAHRVRIVAGDEVRQTRALPADMTVAA
                     DPPPALSVRFEPEGTSTGGDFHLAAGPSRYRVTVDRLTGRVRTERE"
     gene            complement(4578..5003)
                     /gene="gspG"
                     /locus_tag="VLF19_05710"
     CDS             complement(4578..5003)
                     /gene="gspG"
                     /locus_tag="VLF19_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HSE92781.1"
                     /translation="MRNQAGFTLIELLVVIIVLGLLVGLVGPRLFGQVGKSKQGVARA
                     QIELLGATLDQYRLDVGRYPEGAQGLDALQRNPGSVTGWNGPYLKKDVPKDPWGNPYK
                     YRCCPGQHGEYDLWSEGGDGVPGGEGENADVTSWESGKT"
     gene            complement(5111..6322)
                     /locus_tag="VLF19_05715"
     CDS             complement(5111..6322)
                     /locus_tag="VLF19_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSE92782.1"
                     /translation="MPVFVYRAADRRGQTIDGVMEAPDARSVVERLQKEAYYPIRVSA
                     HAERRGWASLPASGRVRQRDLLALTQQLATLFEAGVPLDRALAILEELTPGARLKTIV
                     ADLLRTVRGGGSLSDALAKHHPRPFSRLYINMIRAGEKGGVLEVTLRRLAEFLESRAA
                     FQEAVVSALIYPLLVTTVGAGAIVFLLTFVIPRFASIFADLKQTIPLPTQILLAVSGT
                     VREYWWVGLLVGLGGVLAWRMWTGTPEGRLQWDRTALRLPRVGSLTTKIETARFTRTL
                     GTMLKSGVPIIGALAVVGEMMSNQVVGRAVARLSDSVRRGRTLATAMGEQALFPALAV
                     HMVRVGEETGRLEDMLLKTAETFESDVRTELKRFIGLLEPAIILAMGVLVAFIVVAML
                     MAIFSINEIGL"
     gene            complement(6328..8022)
                     /gene="gspE"
                     /locus_tag="VLF19_05720"
     CDS             complement(6328..8022)
                     /gene="gspE"
                     /locus_tag="VLF19_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HSE92783.1"
                     /translation="MAIATATRLLGEILVADGVTTPDMIVLALSRAQSTGERIGEALV
                     ALGAVTKEDVLRALARQQNVPYLAREEMPSPLPVVKNLSAKYLRQYVVCPVSVEGNVL
                     TVATAEPRNPVVIDDLRQFTGLSVRVVVSTPESILEAIDRTYDGAPTPLQRIVEGVED
                     DRASDDPEDVNHLRDMAFEAPVIRLVNLLVENAIAAEASDIHIEPFEDTLRVRYRVDG
                     VLFDQESPPRRLRDAVTSRIKLMSEMNIAERRLPQDGRIRVSLHGRRVDIRVSTIPTV
                     HGESIVMRLLDRASVFLPLEKLGFAAATLRQFEKLIERPHGIILVTGPTGSGKTTTLY
                     AALEKINSPDRKIITVEDPVEYQLKGVNQIAVKPKIGLSFATGLRHIVRQDPDVILVG
                     EIRDLETVEIAIQASLTGHLVFSTLHTNDAPGAITRLQDMGAEPYLIASVLEAVLAQR
                     LVRRICQACREPHVPIAADLEALGVAFDAATKLWRGRGCEECRGTGYRGRTGIYELFP
                     ITEDVRSLVLRRASSREIRRHAVEAGMVTLRLDGWVRALQGVTTVEEILRVTQEDA"
     gene            8040..8771
                     /locus_tag="VLF19_05725"
     CDS             8040..8771
                     /locus_tag="VLF19_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HSE92784.1"
                     /translation="MLAQWVLVAVAAAADLAGAALVTNAHRRGRAPLHYFVALGAGFM
                     LAAAFVRMLPEATQVPHAFLFVLVGYFGVHLFEHTVAPHFHFGEEVHADAFLRPSAGW
                     LAVLGLGVHTLFDGVAIAAGFMIDTVLGTLLFLAVLLHKLPEGFAVASIVLATGRSRA
                     AALAAGAALGVSTVLGAVLTSLLARDHVGYALALSAGVTIYVAASDLIPEVNREGGPA
                     LAWTVFGGLVLFALADWTLAPSGGH"
     gene            8797..9291
                     /gene="aroQ"
                     /locus_tag="VLF19_05730"
     CDS             8797..9291
                     /gene="aroQ"
                     /locus_tag="VLF19_05730"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HSE92785.1"
                     /translation="MTPAAALVLHGPNLNLLGTREPAIYGSVSLAEVDRLIREHARRR
                     GLRVECRQSNAEGQLIDWLQAAERERFGGVVLNPGAFTHYSIALRDVVAAIRVPVVEV
                     HLSNIHAREEWRHRSVIAGAARGQIAGFGPASYLLGLDALLTVRRPVPRTARPPRSPG
                     SRRA"
     gene            9350..10372
                     /locus_tag="VLF19_05735"
     CDS             9350..10372
                     /locus_tag="VLF19_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSE92786.1"
                     /translation="MDSDDQALVERSRNGDLAAFEPLVEKYRERVWRLAYNYLRDREE
                     AWDVAQEAFVRAWQALPSFRGQSAFYTWLFRIVVNVATDRARPRAARGRAFGTETVPE
                     EEWERVMVDQEAGPEDTAARAEQRERIRRGLEALPEHHRAIIMLSDLEGLSYREIAQV
                     LQIPMGTVMSRLHNARKRLREVLRPLLVALLVVLASLALPASAPAQQVVRFGARVLLA
                     SDGPPPPGTRLSPPGPDARLEAFLPRLRQLFRYREYASLERFLAEVPVGTTQRWSVPG
                     DRQLEVTPEAVAAGAVRLRVRLQRGSMSEVSTNIQAARGNPAVIGGPRYADGVLIIIV
                     WANANP"
     gene            10396..>10863
                     /locus_tag="VLF19_05740"
     CDS             10396..>10863
                     /locus_tag="VLF19_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSE92787.1"
                     /translation="MSDGEARYFRERARIEQGHARYRNKLRAEGKLFVRQRLALLLDP
                     GTEFQEDWLFARSQEAETPADGVVTGVGTVGGRPVAVMANDYTVKAGSWGEKTVQKIV
                     RIQEKATRLGVPLLYLVDAAGGRISEQIRIFPGRFHAGRIFYNEVQLSGVVPQV"
BASE COUNT         1431 a         3883 c         4026 g         1523 t
ORIGIN      
        1 gcgctcgttc agctccccga cccgcgcgtt ggtggtctcc agatccgtgg cgatcgtggc
       61 cttgcgcgcg acgagctgtt gccgtcgtgc gagcacctcc tcgcgcgccg ggaccagttc
      121 ggccgccgcc cggtggcgct cccgcagcgg ctgcacgacg ccctcccagc cgaggacgac
      181 gacggccgcg aggacgccga ggctgatgag cacccgctcg cggcgggaga gattgccgat
      241 caccgcggtg ctccgggcgg caacgggacg gaacggggcg gctggacggc cggcgtccca
      301 tcggggagtg tcggggaggg taccggctgc accccggccg gtggccgtgg gccttccggt
      361 accggaggtg tgggcgcgac accgatcgtc ggcgccgggg cgagggccgg cggcgccggc
      421 gcgggcgtga gcgcggccgg ggggaccggc gccgtgggtg gcgtgacgcc gggggcgacg
      481 ggcggtgggg gcccgctcgt gatggcgccg ggcgtggcgc ggggctgggc gccaggcgca
      541 cccggcatgc tgcgcccggg ggacgcaggc gcgagggcga ccgaccccgc gcccccttcc
      601 cacgccgcgc ggatgcggaa ctgctcgcgg tcgcgtccgc gcgtgacggg cgacgagaac
      661 tcgacgcgct cgaggtgcga ggagttctcg agcaggggaa tgagggccga cgccgccccc
      721 gcctgtcccg tgagctcgat gcccttgccg tcgagcgaga gcatcgtcag ccaggcgtcg
      781 ctcgggagca ggtcggtcag ctcgcgcagg acgggcaggg gccgcaccga cgccgactcg
      841 atcgattgga cggtgccgag catccgccgt ttgcgctcga ggtcctgcat gaccttctcg
      901 acggcgcgca cctccggatc gagcgccgcg atgcgcgcgt tgacggcggc caggcgccgg
      961 ccctcgcgcc agccgggaac gagcagcgcg acgatgccca ggatcacgca gagcgccgcc
     1021 agaccggccg tgacgagctg ggtccgcgtg agacgccgcg ggcggagcgc cctcggaagc
     1081 agctcgagcg gccgcgtgcg cggcccggcg gcgaccgcga ccgcgagctc ggcgagcccg
     1141 ggcgagccgt cgggggagac gccggcgagc agccgccggg cggcggccgt gtagggcggc
     1201 tcgccgaccg gggcgccgag cgcgtgcagc ggtcccgccg tctcggtctc gcggtcaccg
     1261 gagacccaga tcgcgtcgca ctcgcgccag cgcgccaccg tgaagctccg gcggatctct
     1321 tcggcgacga ggtcgtcgtc ggcgtcggcg acgctgcggc tgagcacgag cgcgccggcg
     1381 tcgatgaaga gcaggtcggc gccggtgccg gtgcggtgga cccacaccac gtggccgcga
     1441 cgcggtcggg cgacgagcgc aagcaggttg tgcgcggcga cggtgagcga gacggggcgg
     1501 agcttggcgt cttccgcgac gcgcagcgtg ctctccacga ggcggcggtc cgcggccgcg
     1561 atgaggacgc gctgggcacc gccgtccccg tcggacgcga ggggcacgaa atcgaagggc
     1621 gtgtcctcgc cgccgccgta cgggaggtgg cgctcaagct cgaagcgcac catgtcgtcg
     1681 gcctcgccgg ccaccgcggg gaggtcgatc ggcttgacgg tcacggcgga acgcggcagc
     1741 ccgatccaca ccgcgcggac accgagacgg cgctggtcga gctctgcccg cagtgcggcc
     1801 gcgggggtct cggcgtccac cacgaacgtc tcgacccggc gccgctggat caccgccgcg
     1861 accagccgat cgccgagggc gaggatggcg aaccgggtgg gattgaatct caccgcggtc
     1921 cagagtaaca cgccggcggc cggcaaccgc ctaacgaccg gcccaccact cgaggtacgc
     1981 cacggcggcc ccacccgtgg ctccgcccgc ccccgtggcc ccgcccccac ccgtggcccc
     2041 ggccgcccgc cgctggacga tggccgtgac gcgggcctgg acgcggtcgt ccacgagccc
     2101 ttcggcctcg atgcggaagg tctccgtgtt caccgtgaag ccgcggccgc cgaactgccc
     2161 cggcatctgg aagtacggct cgtcacggcg ggcctgcacg atgaggctga cctcggcatc
     2221 ggacagaccg tgggcgcgca gcacctgctc gctggcggtg ttgatgttca cctggccgcg
     2281 gcccttcgcg ctcacgaatt cggcgaggcc gggcttgccc tcgctgccgc ggaagagcgc
     2341 cggcgtcacc ccccggatct ggagcagctc ggtcaccgac tcgaggttcg cgttccggga
     2401 gcggtagggc acgggcagct tcaggtagta gtcgtcgctc tccgcgccgt tgacgcggtg
     2461 ctcctcgttc ggatcgcgcc agtccgtcac cgagtccacg atcacgccgc gctccgactt
     2521 gtcgagcccg agctgctgga gcaggcggtc cagccgatcc ggcggagccg tgttcacgtt
     2581 gagacgggcc gcctcgtcgg tgatccggta ggtgaagtgg cccgcgccga gcggcacctt
     2641 ctcacgcgag agccgcggtc gggccgtgcg ctcgcgggtg tagaaggtca agacacccgc
     2701 ctcgtccatg cccacgacga ccggcgcggc ggcggcggcg acgatctccc ggatcgcctg
     2761 ctcgaccgcg gcctcggcca ggtgggtggc ggcgatcccg tcgcgccacg cccgcgcggc
     2821 gctggcctcg agccgcatcg agtacgcgaa ctccgcgccg accatgccga gcagggcgac
     2881 gacgaggagt acggccagca gagcgaagcc gcgctcctcc cgcgggcggg cctcgcgcat
     2941 cacggcgtca ccgtgcggag ggagaccgtg agcggcggct gcgcctcggt gcggtcaccg
     3001 cgccggatcg ccagcgcgat gcgcacggcg ctgggcaggc cgccctgctc ctcggcgtcc
     3061 cattcttcct gccagctccc ggcggcgtcg aggtaatgca gcgcgagcgt ctggatcgac
     3121 gggtcgcgca ggaccacctc ggcctcggtg aaggggttgc ggttggggag cggccgctga
     3181 cgcaccacga gctcctcgcc ctcctcgcct cgctcgatgc tcaggaccag cgcggtgaag
     3241 gcgacgggcg ccgcggcggg gaacggaacc gcccgcgtga cgagctcgag ccggtccgcg
     3301 ctgccccgga acaggagcac ggggtcgggt gcgtcgccgg cagaggcgcg gtacggatag
     3361 gcggcgccga cggagcgcga gagaaccatg gtcacgccgc gcaggtgctg gtggacctcg
     3421 gcgcgatcct cgcctcgggt ccacgccgcg agcgcgacgc ggaggccgcc gaaggcgatc
     3481 acgagcagcg cgccgacgat gccgagcgcg atcatcagct cgagcagcgt gaagccccgc
     3541 gcgccgcggc ccggcatcag cgtcctcccg gtgtggtggc tggccggttc cccgggaacg
     3601 tgggtgtccg cggcgaggtc gtcccgggac gccgggtcgc cgtgccgccc ggcgcgctcc
     3661 gggcgcccgg cgtggtcggc gtgcccgagc cggtcagtgt gcccggcgcg ctcgcgctgc
     3721 ccgcgggggg tgtgcccggc gtcgggacct ggccgggcga cgtcgcctcc ggcgtggagg
     3781 tgacggcggc cgtccgcagc gtcgtcacct cgatctgtct gcgctcgtcc cagcgcacgc
     3841 tcacggcgat ctcccagaca cggaaccggg gcgtcgcgcc gtcgtcggga gtcaggtccg
     3901 gcgtctcgag caggcgcgtg gtccgccgcc accggaactc gccctcggcg ccctcgtcct
     3961 gcatctcctc cggcgtcacc acttcgcgtg tcttgaggtc ggccaccagc atcgcctgct
     4021 ggtggtcgcc ggagagcttg agcagccgta gaccctgcgc gaagccctgg atggcggcga
     4081 cgacggcgat accgaggatg gcgagggcga ccagcacctc gatgagcgtg aagccctcct
     4141 cgcgtcgccg cctcactcgc gctccgtgcg gacgcgaccg gtcagccggt cgacggtcac
     4201 gcgataccgg gacgggccgg cggcgaggtg gaagtcccca ccggtcgagg tgccctcggg
     4261 ctcgaagcgc accgagaggg caggcggggg gtcggcggcg accgtcatgt cggcgggcag
     4321 cgcgcgcgtt tgacgcacct cgtcgcccgc gacgatgcgc acacggtgag cgcgcggctc
     4381 cacgatcagg tcgtgggcgc tccgtgtcgt gatcgcctgc tcgcgcgcgt gccgcagcag
     4441 ggcggcgaac cgagcgacct cggtccgcgt gcggatcgtc gccgtgctgc gaccgacggc
     4501 gggggccacg agagcgacgg cgagtcccag caccatcagc acgatgacga gctcgaggag
     4561 ggtgaaccct ctcgcgtcta ggtcttgccg ctttcccatg acgtcacgtc ggcgttctcg
     4621 ccttcaccgc cgggaacgcc atcgccgccc tcggaccaga gatcgtactc gccgtgctgt
     4681 ccggggcagc agcggtactt gtacgggttg ccccaggggt ccttgggaac gtccttcttg
     4741 agatagggcc cgttccagcc cgtcacgctg cccgggttgc gctggagcgc gtccagcccc
     4801 tgcgcaccct ccgggtagcg gccgacgtcg agccggtact ggtcgagggt ggccccgagc
     4861 agctcgatct gcgcccgcgc gacgccctgc ttcgacttgc cgacctggcc gaagagtcgc
     4921 ggcccgacga gccccacgag caacccgagc acgatgatga ccaccagcag ctcgatcaac
     4981 gtgaaccccg cctgattccg cagcgccgcc agcggcccgg tcccgcgtct gtggcccgat
     5041 gtccgcgccg acgtcaagtc ggagaccggg gggcgatgcc gctgtggctg tcgggcggac
     5101 gtcgatgcgc tcacaacccg atctcgttga tcgagaagat ggccatgagc atcgccacga
     5161 cgatgaacgc caccagcacc cccatggcca ggatgatcgc gggctcgagt agcccgatga
     5221 atcgcttgag ctccgtccgc acgtcgctct cgaaggtttc ggcggtcttc agcaacatat
     5281 cctccaggcg gccggtctcc tcgccgacgc gtaccatgtg caccgcgagc gccgggaaga
     5341 gggcctgctc gcccatcgcc gtggcgagcg tgcggccccg gcggacgctg tcggacagtc
     5401 gcgccaccgc gcgcccgacg acctggttcg acatcatctc gccgacgaca gcgagcgcgc
     5461 cgatgatcgg cacgccgctc ttgagcatcg tcccgagcgt ccgcgtgaag cgggcggtct
     5521 cgatcttcgt ggtcaggctc ccgacgcggg gcaatcgcag cgccgtccgg tcccactgca
     5581 ggcgtccttc cggcgttccc gtccacatcc gccacgcgag cacgcccccc aggccgacga
     5641 gcagccccac ccaccagtac tcccgcaccg tgccactcac cgccaggagg atctgtgtcg
     5701 gcaggggaat cgtctgcttg aggtccgcga agatcgacgc gaagcgcggg atgacgaagg
     5761 tcagcaggaa gacgatcgcg ccggccccga ccgtggtgac gagaaggggg tagatcaggg
     5821 ccgagacgac cgcctcctgg aaggccgcgc ggctctcgag gaactcagcg agccggcgca
     5881 gcgtgacctc gagcacgcca cccttctcgc cggccctgat catgttgatg tacaggcgcg
     5941 agaacgggcg ggggtggtgc ttggcgagcg catccgacag ggagccgccg ccgcgcaccg
     6001 tgcgcagcag gtcggcgacg atggtcttga gccgcgcgcc cggtgtcagc tcctcgagga
     6061 tggccagcgc ccggtcgagg ggcacgccgg cctcgaagag ggtggcgagc tgctgggtga
     6121 gggcgagcag atcgcgctgg cgcacgcgcc cggacgcggg caggcttgcc cagccacggc
     6181 gctcggcgtg cgccgacacc cggatcgggt agtacgcctc cttctgcagc cgctccacga
     6241 cggagcgggc gtcgggcgcc tccatcacac cgtcgatcgt ctgcccccgg cggtccgcgg
     6301 ctcgatagac gaagacgggc atcgcgctca ggcgtcttcc tgggtcacgc ggaggatctc
     6361 ctccacggtg gtgacgccct ggagggcgcg tacccagccg tcgagcctca gcgtgaccat
     6421 gcccgcctcc acagcgtggc gccggatctc gcggctggag gcgcggcgga gcacgaggct
     6481 gcgcacgtcc tcggtgatcg ggaacagctc gtagatgccg gtgcgcccgc ggtagcccgt
     6541 gccgcggcac tcctcgcagc cgcggccgcg ccagagcttc gtggccgcgt cgaaggcgac
     6601 cccgagcgcc tcgaggtcgg ccgcgatcgg cacgtggggc tcgcggcacg cctggcagat
     6661 gcggcgcacg aggcgctgcg cgaggacggc ttccagcacg gaggcgatga ggtagggctc
     6721 ggcgcccatg tcttggagtc gggtgatcgc gccgggcgcg tcgttcgtgt gcagcgtgga
     6781 gaacaccagg tggcccgtca gcgaggcctg gatggcgatc tcgacggtct cgaggtcgcg
     6841 gatctcgccg accaggatca cgtcggggtc ctggcggacg atgtgccgga gcccggtggc
     6901 gaagctgagg ccgatcttgg gcttgaccgc gatctggttc acgcccttga gctggtactc
     6961 gacgggatcc tcgacggtga tgatcttgcg gtcgggggag ttgatcttct cgagtgcggc
     7021 gtagagcgtg gtggtcttgc cggagcccgt ggggccggtg acgaggatga tgccgtgcgg
     7081 ccgctcgatg agcttctcga actgccgcag ggtggcggcg gcgaagccca gcttctcgag
     7141 cggcaggaac acgctggccc ggtcgaggag ccgcatcacg atggactcgc cgtgcacggt
     7201 ggggatcgtc gagacgcgga tgtccacgcg ccggccgtgg agcgacacgc ggatgcggcc
     7261 gtcctgcggc aaccggcgct cggcgatgtt catctcggac atcagcttga tccgggaagt
     7321 gacggcgtcc cgcaggcggc gcggcggcga ctcctggtcg aacagcacgc cgtcgacgcg
     7381 gtagcggacc cgcagggtgt cctcgaaggg ctcgatgtgg atgtcggagg cctcggcggc
     7441 gatggcgttc tcgacgagga ggttgaccag gcggatgacc ggcgcctcga aggccatgtc
     7501 gcgcaggtgg ttgacgtcct cggggtcgtc cgacgcgcgg tcgtcctcga cgccctcgac
     7561 gattcgctgc agcggggtgg gcgcgccgtc gtacgtgcgg tcgatcgcct ccaggatgct
     7621 ctcgggcgtg ctcaccacga cccgcacgga cagcccggtg aactggcgga ggtcgtcgat
     7681 cacgacgggg ttccgcggct cggccgtggc caccgtgagc acgttgccct ccacgctcac
     7741 cgggcacacg acgtactgcc ggaggtattt cgccgagagg ttcttgacca ccggcagcgg
     7801 cgagggcatc tcctcgcgcg cgaggtaggg cacgttctgc tggcgcgcga gcgcgcggag
     7861 cacgtcctct ttcgtcaccg cgcccagcgc caccagcgct tcgccgatgc gctcgccggt
     7921 ggactgcgcg cggctcagcg ccagcacgat catgtcgggg gtggtgacgc cgtccgcgac
     7981 cagaatctca ccgaggagcc gggtcgctgt cgcgatggcc atgctatgct ccgtcgcgta
     8041 tgttagcaca gtgggtgctc gtcgccgtcg cggcggctgc ggacctcgcc ggcgccgcgc
     8101 tggtgacgaa cgcccaccgc cggggccgcg cgccgctcca ctacttcgtg gcgctcggcg
     8161 cgggcttcat gctcgccgcg gcgttcgtcc gcatgctccc cgaggccacc caggttcccc
     8221 acgcgttcct cttcgtgctg gtcggctact tcggcgtcca cctcttcgag cacaccgtcg
     8281 cgccgcactt ccacttcggc gaggaggtcc acgccgacgc cttccttcgc ccctcggcgg
     8341 gctggctcgc cgtgctcgga ctcggcgtgc acacgctctt cgacggcgtg gccatcgcgg
     8401 cgggcttcat gatagatacc gtgctgggca cgctcctctt cctcgcggtg ctcctgcaca
     8461 agctgcccga gggcttcgcg gtcgcgtcga tcgtgctcgc caccggccgc tcgcgcgcgg
     8521 ccgccctcgc cgcgggggcg gcgctcgggg tgtccaccgt cctcggcgcc gtgctcacga
     8581 gcctgctcgc ccgcgaccac gtcgggtacg cgctggcgct gtcggccggc gtcacgatct
     8641 acgtcgcggc ctcggacctg atcccggaag tcaatcgcga gggcggcccc gcgctcgcct
     8701 ggacggtctt cggcgggctc gtgctcttcg cgctcgcgga ctggaccctg gcgccgtccg
     8761 gcggccactg aaccgatcgg tgatatcctc gccgcgatga cgcccgctgc cgcgctcgtg
     8821 ttgcacggcc cgaacctgaa cctgctcggc acccgcgagc ccgcgatcta cgggtcggtg
     8881 tcgctcgcgg aggtcgaccg tctgatccgt gagcacgccc ggcgccgggg cctgcgtgtc
     8941 gagtgccggc agtccaacgc cgaaggccag ctcatcgact ggctgcaggc cgccgagcgc
     9001 gagcgcttcg gcggcgtggt gctcaacccc ggcgccttca cgcactactc gatcgccctg
     9061 cgcgacgtgg tggcggcgat ccgcgtcccc gtggtcgagg tccacctctc caacatccac
     9121 gcgcgcgagg agtggcgtca tcgctcggtc atcgccggcg ccgcgcgagg gcagatcgcg
     9181 ggcttcgggc cggcgagcta cctgctcggc ctggacgccc tcctgacggt gaggcgtccc
     9241 gttcctcgaa cagctcgccc gccgcggtcg ccgggctcgc ggcgcgcgtg aatcacgtcc
     9301 gccgcccccg cgtctaaccg gtcggcgcgg ggtgagcgaa cgacgccccg tggacagcga
     9361 cgaccaggcc ctcgtcgagc gcagccgtaa cggcgacctg gcggccttcg agcccctggt
     9421 cgagaagtac cgcgagcggg tgtggcgtct cgcctacaac tatctccggg accgggagga
     9481 ggcgtgggac gtggcccagg aggcgttcgt gcgggcgtgg caggccctgc cgtcgttccg
     9541 ggggcagtcc gcgttctata catggctctt tcgcatcgtc gtcaacgtcg ccaccgaccg
     9601 cgcgcgcccg cgcgcggcgc gcggccgggc cttcggcacc gagacggtgc cggaggagga
     9661 gtgggagcgg gtgatggtgg accaggaggc ggggccggag gacacggcgg cgcgcgccga
     9721 gcagcgggag cggatccggc gcggcctcga ggctttgccc gagcatcacc gcgcgattat
     9781 catgttgagc gatctcgaag gactgtcgta ccgcgagatc gcccaggtac tgcagatccc
     9841 gatgggaacg gtgatgtcgc gtctgcacaa cgcgcgcaag cggctgcgcg aggtcctgcg
     9901 gccgctgctg gtggcgctcc tggtcgtgct cgcctcgctg gcgctcccgg cctcggcgcc
     9961 ggcccagcag gtggtgcggt tcggcgcccg cgtgctgctc gccagcgatg ggccgccgcc
    10021 ccccggcacg cgcctgtcgc cgccgggccc cgacgcgcgg ctcgaggcgt tcctgccgcg
    10081 cctccgtcag ctcttccgct accgcgaata cgcctcgctc gagcgcttcc tggccgaggt
    10141 gccggtgggc acgacccagc ggtggtcggt gccgggcgat cggcagctcg aggtcacgcc
    10201 cgaggccgtg gccgccgggg ccgtgcgcct gcgcgtcagg ctgcagcggg gcagcatgag
    10261 cgaggtctcg accaacatcc aggcggcccg gggcaacccc gccgtgatcg gggggccgcg
    10321 ctacgccgac ggcgtcctca tcatcatcgt ctgggccaac gccaacccct gagcggcggc
    10381 cgacgtgttg acgcggtgag cgacggcgag gcgcgctact tccgggagcg ggcccggatc
    10441 gaacaggggc acgccaggta tcgcaacaag ctgcgcgccg agggcaagct cttcgtccgt
    10501 cagcgtctcg cgctcctgct cgatcccggc accgagttcc aggaggactg gctcttcgca
    10561 cgctcgcagg aggcggagac gcccgccgac ggcgtggtca cgggtgtggg gacggtgggc
    10621 ggccggccgg tggccgtcat ggccaacgac tacacggtga aggccggttc ctggggcgag
    10681 aagaccgtcc agaagatcgt ccgcatccag gagaaggcca cgcggctcgg ggtgccgctg
    10741 ctctacctcg tcgacgcggc cggcggccgc atctccgagc agatccgcat cttccccggg
    10801 cgcttccacg cggggcggat cttctacaac gaggtacagc tctccggcgt ggtgccgcag
    10861 gtg
//
LOCUS       DATIAZ010000165        12638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_174743, whole genome shotgun sequence.
ACCESSION   DATIAZ010000165 DATIAZ010000000
VERSION     DATIAZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174743"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VLF19_05745"
     CDS             <1..478
                     /locus_tag="VLF19_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92788.1"
                     /translation="HELATTLWWATRPADADVPGGLVDLYLVVNAVDDVVPGAYRYWP
                     DTHALETLARGEFRHQAAYLTLEQALGGDAAATIFFLAPLDGLLGAWGDRGYRLANLE
                     AGLTGGRAYLAAYGQRFGASGLTFYDREVVRFFSPHAEGLDAIFVTALGRAGKSPW"
     gene            489..1628
                     /locus_tag="VLF19_05750"
     CDS             489..1628
                     /locus_tag="VLF19_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSE92789.1"
                     /translation="MKKYQLMAPGPTPVPSQVLLAMAQPMIHHRTPEYEALFVEVRAG
                     LKRLFQTAQDVIPLTCSGTGAMEAAVVNTLSPGDTVAIVRAGKFADRWAELAKAYGLS
                     AIDLAAPFGQTVAPARVGEALRSRPEIKAVLVQHSETSTGVLHDVRGYAEATRGTDAL
                     LIVDAVSSLGIADLPMDAWGVDVVVSGSQKGLMLPPGLGFCALNDRAWARAATARLPR
                     YYLDLAEERTALGRNEAHFTPAVSIVVGLREVLRMLEAEGLASIFKRHDRLARATRAG
                     VEALGLELFARATPSPAVTAVVAPKGIDGERIVAAYSEQHNITIAGGQGEMKGQIFRL
                     GHMGYAAEFDVIAALSALEQVLADLGLPVDFGASVRAAQKVFAAR"
     gene            1700..3262
                     /locus_tag="VLF19_05755"
     CDS             1700..3262
                     /locus_tag="VLF19_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92790.1"
                     /translation="MATTAPVRTDAVPTAAYAHLEERILARDQIGATQALYGLLKAGR
                     PVTEVLGQTVRIHAPYTHVPYHQRLDDGVVKFVNNDHCLLSERVGLPLMSMVRPELRL
                     LPLAQTVWYMPTGLDPWNQLIGKAPGHYTRLYNIAVNQAPPMPEVHWPDQEPLHVEGP
                     LRERLNHWLTLVQRGDVVTSYRVFLGLMDDVPNRREVLAHLAFAGLIDVQDRMLHNRS
                     YTTGHKSYRARATIELGEALGWDRAHHVLYAGVPDIAVGPRWYSTYEMGCNVVQNLLD
                     GRDRELLAQDAALTPAEEAMLVDAITRQRESSMIEALVALLKAGRGPRRILDAIQVAS
                     AQVILDTGAPNNFSMAQHGYEYCNTLGWFYDTFAHPHRLKLLFVAASFINRAADHVAS
                     TPDNGRRSITPPPGAAALAPGRLLARLEESLLALRADEAVDLTAAYLEAGFDRAPLLE
                     ALATAACKLGNDPHNQELGLCLLEDYRRTTATDRERLLLACARHTAGHRKYGDPLEAY
                     RRFAEAFDLVAR"
     gene            3392..4423
                     /locus_tag="VLF19_05760"
     CDS             3392..4423
                     /locus_tag="VLF19_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSE92791.1"
                     /translation="MRIGFAFNKKPALVASSSPPSLPSPLDDRYAEWDDEDTIARVER
                     ALSAAGRVVRLEADVDFPLRLREARPDIVFNVAEGMDGPNREAHVPAICEFWSVPYTG
                     SDPMTLSLCLDKGRAKEILAYHRIPTAAFAVVHGPDDLNGFAEWPAIVKPVHEGSSKG
                     ITTDSYCETRAHARDAIAAVVARYGQAALIERWLPGREFTCAILGNGARARTLPLVEV
                     DFEALPPGARRLYSYEAKWIWDTPDRPLEIFHCPADVSRDLGRQVERTALAAYHALRC
                     RDWARIDLRCDAQGVPHVLEVNPLPGILPDPAMNSCFPKAARAAGLDYDTMILAVLGA
                     GAARHALKI"
     gene            4518..5462
                     /locus_tag="VLF19_05765"
     CDS             4518..5462
                     /locus_tag="VLF19_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSE92792.1"
                     /translation="MRQLGWSPVVVDFEGNPGWWLDRLIHGGFGLVFNLCEGLGDQGS
                     EEHLAAAALELLGIPMTGARALTLALCQRKDRANAWLAARGVAVPEWTVAQAAKAVRW
                     RRFPAIVKPVAEDASMGVDAGSVVHTSGDLAAALARGFAGWNHLLVQRYVMGREFNLA
                     VVGDRVLPHAEIDFGELPAGLPRVVTYAAKWEPGSAYHAGTVPRVLDADGSSLVRRLD
                     RLARRVWAAVGGSGYGRIDVRMDERGRLFVLDVNPNPDLSADAGLARQAAAAGWSYAD
                     LIAHIVEAAFVQQSAPARQRGRYLVPDALHAVGRVADA"
     gene            5522..6595
                     /locus_tag="VLF19_05770"
     CDS             5522..6595
                     /locus_tag="VLF19_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HSE92793.1"
                     /translation="MSDWQLMLRRSITTVDQLIERFGAEHIDREAAQRAVESFNLGIS
                     PEVLKLIRRPDDPIGRQYVPSPQENEVVDGVADSLAEDADSPVPNITHRYPDRVLLLV
                     SPVCASYCRFCTRRRKVGDPEKIPLNQFDAAFDYIRAHPEVRDVVMSGGDPLMLSDRR
                     IEHFLKTLRAIRHVEIIRIHTRMPSHLPERITPELCQTIRRYHPVWVNVHFNHPAELT
                     PGARQALARLADAGCPLGCQTVLLEGVNDDPAVMKELMHELLRCRVRPYYLYQADVVA
                     GAEHFRTPVEKGLAIMKALRGWTSGLAVPQFVIDAPGGGGKIPLLPNYIEEITDTEIV
                     VRNYAGETYAYPFPRPGVPRPVA"
     gene            6606..7262
                     /locus_tag="VLF19_05775"
     CDS             6606..7262
                     /locus_tag="VLF19_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92794.1"
                     /translation="MSATLPPSGRLVHLPRLTVDRNIFDLRFGRGCATRHCDATCCAT
                     GVTLDVRERDRVLAHAELVQRAMSPGQDPDPAHWFAAEEVEDADFPSGRATHTRAGAT
                     GCVFLDAERRCVLQKASLAAGGGLDLKPFFCTAFPITLAGGVLLVDDEEIRQRARCCA
                     STADGPLTVFDVCAMELEFMLGSEGVAALRRLAAARLTGRRRQRAAAAPDTSHRDSVR
                     "
     gene            complement(7273..8253)
                     /locus_tag="VLF19_05780"
     CDS             complement(7273..8253)
                     /locus_tag="VLF19_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HSE92795.1"
                     /translation="MATKILFAPRQPEAIVDIARSLAPSGFELVVADPGTPEFYQAAA
                     DAEYYLGLARQMGSEFFRSAPKLRLVQLLSAGYDRVDLEAARKARVPVANNGGANAIA
                     VAEHTIMLILAVLKRLVWHHNNIVAGKWRVGDAAATPTYELSGRILGIIGLGNIGKKV
                     ARRAAAFDARIQYYDIERLTEHEEDALGVRFVLLGELLRTSDVVTLHVPLDDTTRKMI
                     GAAELALMKPGAVLINTCRGPVVDEEALHRALSGGQIAGAGLDVMVEEPPAAKHPLFA
                     LPNVTLTPHSAGPTWENWTARFRNGFDNIQRVARGRQPKWVIPELIAADE"
     gene            complement(8286..8627)
                     /locus_tag="VLF19_05785"
     CDS             complement(8286..8627)
                     /locus_tag="VLF19_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92796.1"
                     /translation="MGTFRLYAGPDGQAHIEKIDLDRTPEWKKGLATTQISFREWEVG
                     EVLDWHPAPRRQFVIILSGQLEIGLGDGSRHVFGPGDARLVEDTTGKGHTTRTLGAQP
                     CVTATIPLAQQ"
     gene            complement(8702..9424)
                     /locus_tag="VLF19_05790"
     CDS             complement(8702..9424)
                     /locus_tag="VLF19_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HSE92797.1"
                     /translation="MGMRVRSITAALAAALGSAGCTMASPPAEPRPAPAVIAVSGMGS
                     VSVKPDTALAQLGAEARAATVADATGDVDRRMRAVLERLRALAIADRDVRTVVYAVDP
                     VPTPPRPGEDPTRIASYRVTNVVEVRVRDLATVGRVVDAAVSAGANVLRALRFTLENR
                     EAAEGEARARAVRDATARARQLAEAAGVRLGEILLLTEGVSPRPVLPRGAVLTASSPG
                     PIETGELEIGVTVEVHFRIARP"
     gene            9487..10113
                     /locus_tag="VLF19_05795"
     CDS             9487..10113
                     /locus_tag="VLF19_05795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSE92798.1"
                     /translation="MSLRLFVVRHAESEYSRGRRFTGWRDVPLSADGHRQCAALAGAL
                     GGCGAQAVYASPLQRARATAEAIAAPCGLQVRTDEAFREMGFGDWEGVTAQEAAARFP
                     EDFALWRSAPDRFHRPGGERLAEVAARAARGRDALRAAHPDGTLVLVTHAIVARLLVL
                     DALGLGPERLWSVDATLAGISELEYRDDWVTVHRMNTLTHLAANGGPT"
     gene            10110..11426
                     /gene="hisZ"
                     /locus_tag="VLF19_05800"
     CDS             10110..11426
                     /gene="hisZ"
                     /locus_tag="VLF19_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HSE92799.1"
                     /translation="MRPVVPTQLPKGARIYLPDEAERKRYVERRLFDVFGRWGYREVV
                     TPTFEFFDAIAVGTDPGLQENMFKFVDRETGRMLALRADITPQIARVVATRLRDEPKP
                     LRLGYVTNVFRYDEPQLAHYREFYQAGVELIGLDKPEAEVEVIAMTVEGLRALGLSRF
                     QIDVGHPDFFRGLMEEVKTDSTRAREVRTALARKDLSALERLVAELAPPAHVGDALLA
                     LPTLFGAGEVLDRAAALARNARSERAVANLAEVHRLLGIYGLAEAVLLDLGEVRGFDY
                     YSGMYFEAYVADFGASVAAGGRYDHLLGRFGYECPAVGFAFDVARALSVMEAQGVAVE
                     LPGPDFFIIDSTREKTAALSLARRLRDLGASVARDIVSRGLDESLAYARVQRVRRVLV
                     VGSPKTRAGEMLVMDLAGGGERVVQIEEALGAPERLLGAPRRAGDA"
     gene            11419..>12638
                     /locus_tag="VLF19_05805"
     CDS             11419..>12638
                     /locus_tag="VLF19_05805"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSE92800.1"
                     /translation="MPNIVVVGTQWGDEGKGKIVDVLGPHVKVVARYQGGNNAGHTVV
                     VGHDKYVFHSIPSAILHPGCICIMACGVVIDPGSLIEELESLVQRGVVFDGNLYISKN
                     AHLIMPYHPALDLASEAHLGARRIGTTGRGVGPAYVDKAARLGIRMADLLDERLFREK
                     LEYNVAQKNRILREIYDAQTFSVEAIFQQYRRYAGWLAPYITDTSMLLGRLIDAGASV
                     LFEGAQATMLDVDHGTYPYVTSSSTTAGGAAIGTGVPPTRINGILGVAKAYTTRVGSG
                     PFPTEMQGRIAADIQARGNEYGATTGRPRRCGWFDALVLRYAVRINGLDAVALTKLDV
                     LDQCETIKICTGYRYQGDVLAEFPDEEATLAEAEPIYEEVSGWMSPTSRAKSPGDLPA
                     KARRYLERLEEVIGV"
BASE COUNT         1722 a         4640 c         4448 g         1828 t
ORIGIN      
        1 ccacgagctg gccacgacgc tctggtgggc gacgcggccc gcggacgcgg acgtgccggg
       61 aggcctggtg gatctctatc tggtggtcaa cgccgtcgac gacgtggtgc cgggggccta
      121 ccgctactgg cccgataccc atgcgctcga aaccctggcg aggggtgagt tcaggcacca
      181 ggcagcctac ctgacgctcg agcaggcgct cggcggcgac gccgcggcca cgatcttctt
      241 cctggcgccg ctcgacggtc tccttggcgc gtggggcgac cgcggctacc ggctggccaa
      301 cctcgaggcc gggctcaccg gcggccgggc ctacctcgcc gcgtacgggc agcgcttcgg
      361 ggcgtcgggc ctgaccttct acgaccgcga ggtggtccgg ttcttctcac cgcacgccga
      421 gggcctcgac gcgatcttcg tcaccgcgct cggccgcgcc ggaaaatccc cgtggtagag
      481 tcgaggccat gaagaaatac cagttgatgg cgcccgggcc cacgcccgtg ccgagccagg
      541 tgctcctggc gatggcgcag ccgatgatcc accaccgcac gccggagtac gaggcgctct
      601 tcgtcgaggt gcgcgcgggg ctcaagcggc tcttccagac ggcgcaggac gtgatcccgc
      661 tgacgtgctc ggggacgggc gcgatggagg ccgccgtcgt caacacgctc tcgcccggcg
      721 acaccgtcgc catcgtccgc gccggcaagt tcgccgaccg gtgggcggag ctcgcgaaag
      781 cgtacggcct gagcgcgatc gatctcgcgg cgccgttcgg ccagaccgtg gcgccggcgc
      841 gcgtgggcga ggcgctgcgc agccggcccg agatcaaggc ggtgctcgtc cagcacagcg
      901 agacgtccac gggcgtgctc cacgacgtgc gcggctacgc cgaggccaca cgcggcaccg
      961 acgcgctcct gatcgtggac gcggtctcga gcctcggcat cgccgacctc ccgatggacg
     1021 cctggggtgt ggacgtcgtc gtctccggct cgcagaaggg cctcatgctg ccgcccgggc
     1081 tcggcttctg cgcactcaac gaccgggcgt gggcgagggc cgccaccgcc aggctgcccc
     1141 gctactacct cgacctcgcg gaggagcgca cggcgctcgg gaggaacgag gcgcacttca
     1201 ccccggccgt ctccatcgtc gtcggcctgc gtgaggtcct gcgcatgctc gaggccgagg
     1261 ggctggccag catcttcaag cgccacgacc gcctcgcccg cgcgacgcgg gccggtgtgg
     1321 aggcgctcgg gctcgagctg ttcgccaggg cgacgccgag cccggcggtg accgccgtgg
     1381 tcgcgccgaa ggggatcgac ggcgagcgta tcgtcgccgc ctactccgag cagcacaaca
     1441 tcacgatcgc cggcgggcag ggtgagatga aggggcagat cttccgtctg ggccacatgg
     1501 gctacgccgc cgagttcgac gtgatcgcgg cgctgtcggc gctggagcag gtgctcgccg
     1561 acctcggcct cccggtggat ttcggcgcct ccgtccgcgc cgcccagaag gtgttcgccg
     1621 cgcgctgagc caccttccga ctcggaagac gatgtcgact ccgggctata ctcccggcag
     1681 atccgttcgg gaggccgaca tggcgacgac agccccggtc aggaccgacg ccgtacccac
     1741 cgccgcgtac gcccacctgg aggagcgcat cctggcgcgc gaccagatcg gcgccaccca
     1801 ggcactctac ggcctgttga aggccggccg gccggtcacc gaggtcctcg gccagacggt
     1861 gcgcatccac gcgccctaca ctcacgtgcc ctaccaccag cggctcgacg acggcgtggt
     1921 gaagttcgtg aacaacgacc actgcctgct cagcgagcgg gtcggcctgc cgctgatgtc
     1981 gatggtccgg ccggagctgc ggctgctgcc gctggcccag accgtctggt acatgcccac
     2041 ggggctcgat ccctggaacc agctcatcgg caaggccccc ggccactaca cgcgcctcta
     2101 caacatcgcg gtgaaccagg cgccgccgat gcccgaggtg cactggccgg atcaggagcc
     2161 gctgcacgtc gaggggccgc tgcgcgagcg tctcaaccac tggctgacgc tcgtccagcg
     2221 cggcgacgtc gtgacctcgt atcgcgtgtt cctcgggctg atggacgacg tgccgaaccg
     2281 gcgcgaggtg ctcgcgcatc tcgcgttcgc cgggctgatc gacgtgcagg atcggatgct
     2341 ccacaaccgc tcgtacacga ccgggcacaa gtcgtaccgc gcccgcgcca ccatcgagct
     2401 gggcgaggcg ctcggctggg accgggcgca ccacgtgctc tacgcgggcg tgccggacat
     2461 cgcggtgggc ccgcgctggt actcgacgta cgagatgggc tgcaacgtgg tccagaacct
     2521 gctcgacggc cgcgaccggg agctgctcgc gcaggacgcg gccctcacgc cggccgagga
     2581 ggcgatgctg gtggacgcga tcacgcgcca gcgggagtcc tccatgatcg aggcgctggt
     2641 ggctctcctc aaggcgggga gggggccgcg ccggatcctc gacgcgatcc aggtggcctc
     2701 agcgcaggtg atcctcgaca cgggcgctcc gaacaacttc tcgatggccc agcatggcta
     2761 cgagtactgc aacacgctcg ggtggttcta cgacaccttc gcgcaccctc accggctgaa
     2821 gctgctcttc gtcgcggcct ccttcatcaa ccgggcggcc gatcacgtcg ccagcacgcc
     2881 ggacaacggc cggcgatcga tcacgccgcc acccggggcc gcggccctgg ccccgggccg
     2941 gctcctcgcg aggctcgagg agagtctgct cgccctccgg gccgacgagg ccgtggacct
     3001 gaccgcggcc tatctcgagg ccggcttcga tcgggcgccg ctgctcgagg cgctggccac
     3061 cgccgcctgc aagctcggca acgaccccca caaccaggag ctcgggctct gtctgctgga
     3121 ggactatcgc cgcaccacgg ccaccgaccg tgagcggctg ctgctggcct gcgccaggca
     3181 caccgccggc caccgcaagt acggcgaccc cctggaggcg taccgccgct tcgccgaggc
     3241 cttcgacctg gtcgcgcgct gaccggcgcg ccctccacgt cgaggacgcg gtcgctcgcc
     3301 cgcgcgggtc cggcgaggag gatggggagc gagcgcgatc ggtgaggatt ctgcttccat
     3361 tctcccgggc ggcgtgacta tatttccccg gatgcggatc gggttcgcct tcaacaagaa
     3421 gcctgcgctc gtcgcctcct cctccccgcc gtctctgcct tcgccgctcg acgaccgcta
     3481 cgccgagtgg gacgacgagg acaccatcgc gcgcgtcgag agggcgctgt ccgccgccgg
     3541 tcgcgtcgtg cggctcgagg ccgatgtcga tttcccgctg cgcctgcgcg aggcgcgccc
     3601 ggacatcgtg ttcaacgtcg ccgagggaat ggacggaccg aaccgggaag cccacgtgcc
     3661 cgccatctgc gagttctggt ccgtgcccta cacgggcagc gatcccatga ccctgtccct
     3721 ctgcctcgac aaggggcgcg ccaaggagat tctcgcctac caccgcattc caaccgccgc
     3781 cttcgccgtc gtgcacggcc ccgacgacct gaacggcttc gcggagtggc cggcgatcgt
     3841 caagccggtg cacgagggct cgagcaaggg catcacgacc gattcctact gcgagacacg
     3901 cgctcacgct cgtgacgcca tcgccgccgt cgtggcccgc tatggccagg cggccctgat
     3961 cgagcggtgg ctgcccggcc gggagttcac ctgcgcgatc ctcggcaacg gcgcccgcgc
     4021 gcgcacgctg cccctcgtcg aggtcgactt cgaggcgctg ccgcccgggg cccgccgact
     4081 ctactcgtac gaggccaagt ggatctggga cacgccggac cgcccgctcg agatctttca
     4141 ctgtcccgcc gacgtctcgc gcgacctggg ccggcaggtg gagcgcacgg cgctggcggc
     4201 ctatcacgcc ctccggtgcc gggactgggc gcggatcgac ctgcgctgcg acgcgcaggg
     4261 cgtcccccac gtcctggagg tcaacccgtt gcccggcatc ctgccggatc ccgcgatgaa
     4321 ctcctgcttc cccaaggcgg cgcgggccgc cggcctcgac tacgacacga tgatcctcgc
     4381 cgtcctgggc gccggggcgg ctcgtcatgc cctcaagatc tgaacgtccc cgggccgccg
     4441 tggtcgtcga tgcggccgcg gaggcctccc agcggctcga agcctccgca gggtccctgg
     4501 acgaggtcgt cgcggccttg cgccaactcg gctggtcgcc cgtagtggtg gacttcgagg
     4561 ggaacccggg ctggtggctc gatcgactca tccacggcgg cttcggtctc gtcttcaacc
     4621 tgtgcgaggg gctcggcgac cagggctccg aggagcacct cgccgccgcc gccctggagc
     4681 tgctggggat cccgatgacg ggcgcccgcg cgctcaccct tgccctctgc cagcgcaagg
     4741 atcgggcgaa cgcgtggctg gccgcccgcg gcgtggcagt gcccgagtgg acggtcgccc
     4801 aggctgcgaa ggccgtccgc tggcggcgat tccccgccat cgtcaagccg gtggccgagg
     4861 acgcctcgat gggggtggac gcaggctccg tcgtccacac gtcgggcgat ctcgccgcgg
     4921 ccctggcccg gggcttcgcc gggtggaacc acctgctcgt tcagcgctac gtgatggggc
     4981 gtgagttcaa cctggccgtc gtgggcgacc gcgtcctgcc ccacgcggag atcgacttcg
     5041 gcgagctgcc ggccgggctg ccgcgcgtgg tcacctatgc ggccaagtgg gagcctggta
     5101 gcgcgtacca tgccggcacc gtaccccgcg tgctcgacgc cgacggcagc tcactcgtcc
     5161 gccggctcga tcgcctggcc cgccgggtct gggccgccgt cgggggcagc ggctacggcc
     5221 ggatcgacgt gcgcatggac gagcgtgggc ggctcttcgt gctcgacgtc aaccccaacc
     5281 ccgacctctc ggccgacgcg gggctggccc gccaggcggc cgccgccgga tggagctacg
     5341 ccgacctcat cgcgcacatc gtcgaggccg ccttcgtcca gcagagcgcg ccggcgcgcc
     5401 agcgcggccg gtacctcgtt ccggacgccc tgcacgccgt cggccgggtg gccgacgcat
     5461 gacgcctgac ccgcacaccg ccgacttcgg cgaagcccac gtcactcggg gaggctgacc
     5521 aatgtccgac tggcagctca tgctgcgccg atcgatcacg acggtcgacc agctcatcga
     5581 gcggttcggc gccgagcaca tcgaccgcga ggccgcgcag cgcgccgtgg agagcttcaa
     5641 cctggggata tcgccggagg tcctgaagct catccggcga ccggacgatc ccatcgggcg
     5701 ccagtacgtg ccgagcccgc aggagaacga ggtcgtggac ggcgtcgccg actcgctggc
     5761 cgaggacgcc gactcccccg tgccgaacat cacccaccgc taccccgacc gcgtgctgct
     5821 cctggtgagc ccggtgtgtg ccagctactg ccgcttctgc acgcggcggc gcaaggtggg
     5881 cgatcccgag aagattccgc tcaaccagtt cgacgccgcc ttcgactaca tccgcgctca
     5941 ccccgaggtc cgcgacgtcg tcatgtccgg cggggatccc ttgatgctgt ccgaccggcg
     6001 tatcgagcac ttcttgaaga cgctccgggc catccggcac gtggagatca tccgcatcca
     6061 cacgcgcatg ccatcgcacc tgcccgagcg catcacgcca gagctctgcc agacgatccg
     6121 tcgctaccat cccgtctggg tgaacgtgca cttcaaccac cctgccgagc tcactccggg
     6181 cgcgcggcag gcgctggcgc gcctggccga cgccggctgt ccgctgggct gccagaccgt
     6241 gctcctggag ggcgtcaacg acgacccggc ggtgatgaag gagctcatgc acgagctgct
     6301 gcgctgccgg gtgcggccgt attatctcta ccaggccgat gtcgtcgccg gtgccgagca
     6361 cttccggacg ccggtcgaga agggcctggc catcatgaag gccctgcgcg ggtggacgtc
     6421 ggggctggcg gtgccgcagt tcgtcatcga cgcgcccggc gggggtggca agatcccgct
     6481 gctgccgaac tacatcgagg agatcaccga caccgagatc gtcgtgcgca actacgcggg
     6541 cgagacctac gcctacccgt ttcccaggcc cggcgtgccc cgaccggtcg cgtgagagcc
     6601 gggtgatgag cgccacgctg cccccctccg gccggctggt ccacctgccg cgcctgacgg
     6661 tcgaccgcaa catcttcgat ctgcgcttcg gccgcggctg cgccacgcgc cactgcgatg
     6721 ccacctgctg cgcgaccggc gtcacgctcg acgtccgcga gcgtgaccgg gtgctcgccc
     6781 acgccgagct cgtccagcgc gcgatgagcc cgggccagga cccggatccc gcgcactggt
     6841 tcgcggccga ggaggtcgag gacgccgatt tcccgtcggg ccgcgccacc cacacccgcg
     6901 ccggtgccac cgggtgcgtg ttcctcgacg ccgagcggcg ctgcgtcctc cagaaagcgt
     6961 cgctggccgc cggcgggggg ctcgacctca agccgttctt ctgcacggcg ttcccgatca
     7021 ccctggcggg gggggtcctc ctcgtggacg acgaggagat tcgtcagcgg gcgcgctgtt
     7081 gcgcctccac ggcggatggc ccgctcaccg tcttcgacgt gtgcgccatg gagctcgagt
     7141 tcatgctggg gagcgaaggc gtcgccgccc tccgccgcct ggccgccgcc cggctgactg
     7201 gacgtcgccg gcagcgggcc gccgctgctc ccgacacctc ccaccgtgat tccgtgaggt
     7261 agttgcgtca ggtcactcgt ccgcggcgat cagctccggg atcacccatt tcggctggcg
     7321 gccgcgggcc acgcgctgga tgttgtcgaa gccattgcgg aagcgcgcgg tccagttctc
     7381 ccacgtcggt ccggccgagt gcggcgtgag cgtgacgttc ggcagcgcga agagggggtg
     7441 cttcgccgcc ggcggctcct cgaccatcac gtcgagcccg gcgccggcga tctgtccccc
     7501 ggagagcgcc cggtgcagcg cctcttcgtc cacgaccggc ccccggcacg tgttgatcag
     7561 gaccgcgccc ggcttcatca gcgcgagctc cgccgctccg atcatctttc gcgtcgtgtc
     7621 gtcgagcggc acgtgcaggg tgaccacgtc ggaggtgcgc aacagctcgc cgaggagcac
     7681 gaagcggaca ccgagggcgt cctcctcgtg ctcggtcagc cgctcgatgt cgtagtactg
     7741 gatgcgcgcg tcgaaggccg cggcccggcg cgcgaccttc ttgccgatgt tgccgagccc
     7801 gatgatgccg agaatcctgc cggatagctc gtacgtcggc gtcgccgccg cgtcgcccac
     7861 ccgccacttg ccggcgacga tgttgttgtg gtgccagacg aggcgcttga gcaccgcgag
     7921 gatgagcatg atcgtgtgct cggccaccgc gatggcgttg gcgccgccgt tgttggcgac
     7981 gggcacgcgc gccttccgcg ccgcctcgag gtcgacgcgg tcgtaaccgg cgctgaggag
     8041 ctgcaccagt ctgagcttcg gcgccgaccg gaagaactcg ctccccatct gccgggccag
     8101 gccgaggtag tactccgcgt cggcggccgc ctggtagaac tccggggtgc cggggtcggc
     8161 gaccaccagc tcgaagccgg aaggcgccag cgacctggcg atgtcgacga tcgcctcggg
     8221 ctgccgaggc gcgaagagga tcttggtggc catgagcggt cgtctccttt acggcggtgc
     8281 gaggtctatt gctgcgcgag cggaatcgtc gcggtgacgc agggctgggc gccgagggtg
     8341 cgcgtggtgt gacccttgcc cgtcgtgtcc tcgaccagcc gcgcgtcgcc gggaccgaac
     8401 acgtgcctgg agccgtcgcc caggccgatc tcgagctggc ccgagaggat gatgacgaac
     8461 tgccgccgcg gcgccgggtg ccagtcgagg acctcgccga cctcccactc gcggaacgag
     8521 atctgggtcg tcgccagccc cttcttccac tccggcgtcc ggtcgaggtc gatcttctcg
     8581 atgtgcgcct gcccgtcggg tcctgcgtag agtcggaacg ttcccatcac ggcctcctga
     8641 tcgcgtcgtc ggtgaccggc gggatgctag cacacacccg tcggggtcca gggcacggcg
     8701 gtcaagggcg ggcgatgcgg aagtgcacct cgacggtgac gccgatctcc agctccccgg
     8761 tctcgatcgg gccggggctc gaggccgtca gcaccgcccc gcgggggagg accggcctcg
     8821 gcgacacgcc ctcggtgagc aagaggatct cgcccagccg cacgcctgcg gcttcggcca
     8881 gctggcgcgc tctggccgtc gcgtcccgca ccgcacgcgc ccgggcctct ccctcggcgg
     8941 cctctcggtt ctcgagcgtg aagcggagcg cgcgcagcac gttggcgccc gccgacaccg
     9001 cggcgtccac gacgcgcccg accgtggcga ggtcgcgcac ccggacctcg acgacgttgg
     9061 tcacccggta gctcgcgatg cgggtcggat cctcgccggg gcgcgggggc gtgggcacgg
     9121 ggtcgacggc gtacacgacc gtccggacgt cccggtcggc gatggcgagc gcgcgcagcc
     9181 gctccagcac ggcgcgcatg cggcggtcca cgtcgccggt ggcgtcggcc acggtggcgg
     9241 cgcgcgcctc cgcgcccagc tgggccagcg cggtgtccgg cttgacgctc acgctgccca
     9301 tgccgctgac ggcgatgacg gccggcgctg gccgcggctc cgccggcggc gacgccatgg
     9361 tgcagccggc ggatccgagc gcggcggcca gcgccgcggt gatcgagcgc acgcgcattc
     9421 ccatgcgacc cccccgagcg aggcttcagg atactctcgt ccggcgctag aatggtcggg
     9481 ttgaccatga gcctgagact cttcgtcgtg cgacacgccg agtccgagta ctcccgggga
     9541 cgacggttca cgggatggcg cgacgtgccc ctcagcgccg acggccaccg gcagtgcgcg
     9601 gcgctcgccg gcgcgctggg cgggtgcggc gcccaggcgg tgtacgcgag cccgctccag
     9661 cgcgccaggg cgacggccga ggcgatcgcc gcgccgtgcg ggctccaggt ccggaccgac
     9721 gaggccttcc gcgagatggg cttcggcgac tgggagggcg tgaccgccca ggaggccgcc
     9781 gcgcgctttc ccgaggactt cgcgctctgg cggtcggccc ccgaccgctt ccaccgtccc
     9841 ggcggggagc ggctcgccga ggtcgcggcg cgggccgccc ggggccgcga cgcgctgcgc
     9901 gccgcgcacc ccgacggcac actggtcctc gtcacgcacg cgatcgtcgc gcggctcctg
     9961 gtgctggacg ccctcggtct cgggcccgag cgcctgtggt ccgtcgacgc cacgctggcc
    10021 ggcatctccg agctcgagta ccgcgacgac tgggtgaccg tgcaccggat gaacacgctg
    10081 acccacctgg cagccaacgg agggccgacg tgaggcccgt ggtgccgacc cagctgccca
    10141 agggcgcccg catctacctg ccggacgagg ccgagcgcaa gcgctacgtc gagcgccggc
    10201 tcttcgacgt cttcgggcgc tggggctacc gcgaggtcgt cacgccgacg ttcgagttct
    10261 tcgacgcgat cgcggtcggc acggacccgg ggctgcagga gaacatgttc aagttcgtgg
    10321 accgcgagac gggccgcatg ctggccctgc gcgccgacat cacgcctcag atcgcgcggg
    10381 tcgtggccac gcggctgcgc gacgagccca agcccctgcg cctgggctac gtcaccaacg
    10441 tcttccgcta cgacgagccg cagctcgcgc actaccgcga gttctaccag gccggcgtgg
    10501 agctgatcgg cctggacaag cccgaggccg aggtcgaggt catcgccatg accgtcgagg
    10561 ggctgcgggc gctcgggctc tcgcgcttcc agatcgacgt cggccacccg gacttcttcc
    10621 gcgggctcat ggaggaggtc aagaccgaca gcacgcgggc ccgcgaggtg cgcacggcgc
    10681 tggcgcgcaa ggacctctcg gcgctggagc ggctcgtggc cgagctggcg ccccccgcgc
    10741 acgtgggcga cgcgctcctc gcgctgccga cgctcttcgg cgccggcgag gtgctcgacc
    10801 gcgccgcggc gctcgcgcgc aacgcgcggt cggagcgggc ggtggcgaac ctggccgagg
    10861 tccaccggct gctgggcatc tacgggctcg ccgaggcggt gctgctcgac ctcggcgagg
    10921 tgcgcgggtt cgactactac tcgggcatgt acttcgaggc ctacgtcgcg gacttcggcg
    10981 cctccgtcgc cgccggcggc cgctacgacc acctgctcgg gcgcttcggc tacgagtgcc
    11041 cggcggtggg cttcgccttc gacgtggcgc gcgcgctcag cgtgatggag gcgcagggcg
    11101 tggcggtcga gctgccgggg cccgacttct tcatcatcga ctccacgcgc gagaagaccg
    11161 ccgccctgtc gctggcgcgc cggctccgcg acctcggggc ctcggtcgcg cgcgacatcg
    11221 tcagccgcgg gctcgacgag tcgctggcct acgcgcgcgt ccagcgcgtg cgccgcgtgc
    11281 tggtggtggg cagcccgaag acgcgggcgg gggagatgct cgtgatggac ctcgccggcg
    11341 gcggcgagcg cgtggtacag atcgaggagg cgctgggggc gcccgagcgg ctgctcgggg
    11401 cgccgcggag ggcgggcgat gcctaacatc gtggtggtcg gcacgcagtg gggcgacgag
    11461 ggcaagggca agatcgtgga cgttctgggc ccgcacgtga aggtcgtggc gcgctaccag
    11521 ggcggtaaca acgccggcca caccgtggtc gtgggccacg acaagtacgt cttccactcg
    11581 atcccgtcgg cgatcctgca cccgggctgc atctgcatca tggcctgcgg cgtggtgatc
    11641 gacccgggct cgctgatcga ggagctggag tcgctggtcc agcgcggcgt ggtcttcgac
    11701 ggcaacctct acatctccaa gaacgcccac ctgatcatgc cctaccaccc ggccctcgac
    11761 ctggcctccg aggcgcacct gggcgcccgg cgcatcggga ccaccggacg cggcgtgggg
    11821 ccggcctacg tggacaaggc cgcgcgcctg ggcatccgca tggccgatct cctcgacgag
    11881 cggctcttcc gcgagaagct cgagtacaac gtggcccaga agaaccgcat cctgcgcgag
    11941 atctacgacg cgcagacctt cagcgtggag gcgatcttcc agcagtaccg gcgctatgcg
    12001 ggctggctgg cgccctacat caccgacacc tcgatgctgc tgggcaggct catcgacgcc
    12061 ggcgcctccg tgctgttcga gggcgcgcag gccaccatgc tcgacgtcga tcacggcacc
    12121 tatccctatg tcacctcgtc ctcgacgacg gccggcggcg ccgccatcgg caccggggtg
    12181 ccgccgacgc ggattaacgg catcctcggc gtcgcgaagg cctacacgac gcgcgtgggc
    12241 tcggggccgt tccccaccga gatgcagggg cgcatcgccg ccgacatcca ggcccgcggc
    12301 aacgagtacg gcgccacgac cggccggccg cggcgctgcg gctggttcga cgccctggtg
    12361 ctgcgctacg ccgtccgcat caacgggctc gacgccgtcg ccctcaccaa gctcgacgtg
    12421 ctcgaccagt gcgagaccat caagatctgc accggctacc gctaccaggg cgacgtgctc
    12481 gcggagttcc ccgacgagga agcgacgctg gccgaggccg agcccatcta cgaggaggtc
    12541 tccggctgga tgtcgccgac gagccgcgcc aagagtcccg gcgacctgcc ggccaaggcg
    12601 cgccgctacc tcgagcgcct ggaggaggtg atcggcgt
//
LOCUS       DATIAZ010000166         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_174786, whole genome shotgun sequence.
ACCESSION   DATIAZ010000166 DATIAZ010000000
VERSION     DATIAZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174786"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VLF19_05810"
     CDS             complement(<1..705)
                     /locus_tag="VLF19_05810"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HSE92801.1"
                     /translation="MREPIRITRATARKPRPKDGELGFGQFFTDHMFVMDFEEEKGWY
                     DPRVEPYGPLPLDPATAVLHYGQGLFEGLKAFHGRDARIRLFRPQKHVERLNRTAERM
                     CIPALDPELVLRSWTTLVDVDRDWAPSTVGTSLYIRPTVIASEPFLGVRPSRQYLYFV
                     IVSPVGAYYPEGINPVKIKVIDKYVRAVPGGLGEAKTAANYAASLYAAEEAKHEGFTQ
                     VLWLDAVHRKYVEEVGT"
     gene            complement(827..2476)
                     /locus_tag="VLF19_05815"
     CDS             complement(827..2476)
                     /locus_tag="VLF19_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSE92802.1"
                     /translation="MSDRERWKERTYAPFAAKAPERPVRYETLSGIPVQPLYTPEDLG
                     DWSYTDKLGYPGEYPFTRGVYPTMYRGRLWTMRMFAGFGRPEDTNERFKYLLEQGQTG
                     LSTAFDMPALMGYDADHPRARGEVGKEGVSISTLDDFERLFDGIPLGDVTTSMTINCT
                     ASVALAMYLAVAEKQGVAWDRIGGTMQNDMLKEFIAQKEWICPPEPAVRIVTDMIEFT
                     AKHVPRFNPVSISGYHIREAGSTAVQELAFTLADGLGYVEAAMARGLDVDTFAPRLSF
                     FFDIHNDFFEEIAKLRAARRLWARFMKERYGARKAESMRLRTHTQTAGVSATAQQPLN
                     NVARVALQALAAVLGGAQSLHTNSYDETWALPTEDAVTVALRTQQIIAEESGVPLTVD
                     PLGGSYFLETLTDQMEAAALEYIGRIDAMGGMIRAIDTGYPQKEIADAAYRYQLMDDR
                     GEKVVVGVNKYLMREEKPIHYLRIEEQVELEQIERTRRFKATRDAAQVERRLKQLAEA
                     CRDGRNVMPVLIDAVKDYASLGEISDVYRQVFGLYREPIIF"
     gene            complement(2469..>2943)
                     /locus_tag="VLF19_05820"
     CDS             complement(2469..>2943)
                     /locus_tag="VLF19_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92803.1"
                     /translation="EEGMGFKIAMGSLDGGRIGIAAQALGIAQGAYERAVAYAKERKA
                     FGVPIGHHQMVQWMLADMATAIEGARLLIFRAAALKDAGQPFGTAAAMAKLFASETAM
                     KVTTDAVQVHGGYGFIKEYQVERYFRDAKITQIYEGTSQIQKLVIARAVLGDRHE"
BASE COUNT          428 a         1049 c          966 g          500 t
ORIGIN      
        1 ggtgccgact tcctcgacgt acttgcgatg gacggcgtcg agccagagca cctgcgtgaa
       61 gccctcgtgc ttcgcctcct cggcggcata gaggctggcc gcgtagttcg ccgcggtctt
      121 cgcctcgccg agcccgccgg gaacggcgcg cacgtacttg tcgatgacct tgatcttcac
      181 cgggttgatg ccctcggggt agtatgcgcc caccggcgag acgatgacga agtagaggta
      241 ctgccgggac gggcgcacgc cgaggaacgg ctcgctggcg atgacggtgg gccggatgta
      301 gagcgaggtc ccgaccgtgg acggcgccca gtcgcggtcg acgtccacga gcgtcgtcca
      361 cgagcggagg accagctcgg gatcgagcgc cggaatgcac atccgctccg cggtccggtt
      421 cagccgctcg acgtgcttct gcgggcggaa cagccggatc ctcgcgtccc ggccgtggaa
      481 ggccttgagc ccctcgaaga ggccctgccc gtagtgcagg acggccgtcg ccggatcgag
      541 cgggagcggg ccgtagggct cgacccgcgg atcgtaccag cccttctcct cctcgaagtc
      601 catgacgaac atgtggtcgg tgaagaactg cccgaacccc agctcgccgt ccttgggcct
      661 gggcttccgc gccgtcgccc tcgtgatcct gatcggctcc ctcatctctt ccccctgccc
      721 gccatattca cgcagcgcgc ctcgaccgga ccgccctcga ccgccggcct cgccggcgca
      781 atcccccctg gggaggtctc ggaggcggtc gtccggcccc tccgactcaa aagatgatcg
      841 gctcgcggta gagcccgaac acctgccggt acacgtccga gatctccccg aggctcgcgt
      901 agtccttcac ggcgtcgatc agcacgggca tgacgttccg tccgtcgcga cacgcctcgg
      961 cgagctgctt gagccgccgc tcgacctgcg cggcgtcccg cgtggccttg aaccggcgcg
     1021 tccgctcgat ctgctccagc tccacctgct cctcgatgcg cagatagtgg atgggcttct
     1081 cctcgcgcat caggtacttg ttgacgccga cgacgacctt ctcgccacgg tcgtccatga
     1141 gctgatagcg gtaggctgcg tcggcgatct ccttctgcgg gtagccggtg tcgatggcgc
     1201 ggatcatgcc acccatcgcg tcgatccggc cgatgtattc gagcgccgcg gcctccatct
     1261 ggtcggtcaa cgtctcgagg aagtacgagc cgcccagcgg atccacggtc agcggaacgc
     1321 cggactcctc ggcgatgatc tgctgcgtcc gcagcgccac cgtgacggcg tcctccgtgg
     1381 gcagcgccca cgtctcgtcg taggagttcg tgtgcagcga ctgcgcgccg ccgagcacgg
     1441 ccgccagcgc ctgcagggcc acgcgggcca cgttgttcag cggctgctgc gccgtcgccg
     1501 agacaccggc ggtctgcgtg tgggtgcgca ggcgcatcga ctcggccttc cgcgccccgt
     1561 accgctcctt catgaaccgg gcccagagac gccgcgccgc ccggagcttg gcgatttcct
     1621 cgaagaagtc gttgtggatg tcgaagaaga agctgagccg cggggcgaag gtgtccacgt
     1681 cgagaccccg cgccatcgcc gcctccacgt accccagccc gtcggcgagc gtgaaggcca
     1741 actcctgcac ggccgtcgac cccgcctcgc ggatgtggta acccgagatc gacaccgggt
     1801 tgaagcgcgg gacgtgcttc gcggtgaact cgatcatgtc ggtcacgatg cgcacggccg
     1861 gctcgggcgg gcagatccat tccttctggg cgatgaactc cttgagcatg tcgttctgca
     1921 tcgtgccgcc gatgcggtcc cacgccacgc cctgcttctc ggccacggcc aggtacatgg
     1981 ccagcgcgac cgacgccgtg cagttgatgg tcatcgatgt cgtcacgtcg cccaggggga
     2041 tgccgtcgaa gaggcgctcg aagtcgtcca gcgtcgagat cgacacgccc tccttgccca
     2101 cctcgccgcg agcgcgcggg tggtcggcgt cgtagcccat gagcgccggc atgtcgaagg
     2161 ccgtggagag gccggtctgt ccctgctcca gcaggtactt gaagcgctcg ttcgtatcct
     2221 ccggccgccc gaagccggcg aacatccgca tcgtccacag acgcccacgg tacatcgtcg
     2281 gatagacgcc gcgcgtgaac ggatactcgc ccgggtatcc gagcttgtcg gtgtacgacc
     2341 agtcgccgag gtcctccggc gtgtagagcg gctggacggg gatccccgac agcgtctcgt
     2401 agcgcaccgg acgctcgggc gccttggccg cgaacggcgc gtacgtccgc tccttccagc
     2461 gctcccggtc actcatgcct gtcccccaga accgcgcgcg cgatcacgag cttctggatc
     2521 tgcgaggtgc cctcgtagat ctgggtgatc ttggcgtcgc ggaagtagcg ctcgacctgg
     2581 tactccttga tgaacccgta gccgccgtgg acctggacgg cgtcggtcgt caccttcatc
     2641 gccgtctcgg atgcgaagag cttggccatc gcggccgcgg ttccgaaggg ctggccggcg
     2701 tccttcaacg cggcggcccg gaagatcagc aggcgcgccc cctcgatggc cgtggccatg
     2761 tcggcgagca tccactggac catctggtga tggccgatgg gcacgccgaa cgccttgcgc
     2821 tccttggcgt aggccaccgc gcgctcgtag gcgccctgcg cgatgcccag cgcctgcgcg
     2881 gcgatgccga tgcggccgcc gtcgagcgac cccatggcga tcttgaaccc catgccctcc
     2941 tcg
//
LOCUS       DATIAZ010000167         7419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1490043, whole genome shotgun sequence.
ACCESSION   DATIAZ010000167 DATIAZ010000000
VERSION     DATIAZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490043"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..284)
                     /locus_tag="VLF19_05825"
     CDS             complement(12..284)
                     /locus_tag="VLF19_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HSE92804.1"
                     /translation="MLFMVVETFRNQDARSVYRRLRDRGRMMPDGLKFVSSWVAADLG
                     RCFQLMECDDVTLLQRWVTEWSELVHFEIVPVVAGKETAEALAGLL"
     gene            418..2751
                     /locus_tag="VLF19_05830"
     CDS             418..2751
                     /locus_tag="VLF19_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE92805.1"
                     /translation="MSVTRLLGAAVRRREDPALVQGAGRYVDDLAPPRAVALALVRSP
                     HAHARVRAIDTRAAAAMPGVLAVLTARDLEGRVSPEPAVGIPPNARRPPRPLLASGTV
                     RYVGEPVVAVVAESRYVAADGGEAVLVDYEPLPAVGDPEAAATPGAPRVHEEFADNVA
                     LRWSWTHGDVDAAFARARHVVRLRLVNQRVAGLAMEPRGCVAEYRPGALTVWAGTQVP
                     HRLRSGLAEMLRLPETAVRVIAPNIGGGFGCKIGYYVDEVLCALAAMRLERPVKLVLT
                     RREDFLTTTQGRGQINDVEAAVARDGTVLALRCRTLADLGAYLEALTPYPGMLTGRLI
                     TGPYRIPAAAYELTGVFTNAMATAPYRGAGRPEATYALERTMDEIARVTGLDAAEVRR
                     RNLIRPDEFPYRAPSGLVYDSGQYEVALDRALALADYKRLREEQRRAREEGRLVGIGL
                     SSFMETAGVGPSRIAPMPGWESGSVRVEPSGLVTVLTGTSPHGQGLETAFAQVAADRL
                     GVPIDDVAVLHSDTAVVPAGVGTFGSRSIAVGGPAIAVSVDKILDKARRIAAALLEAA
                     PEDLIWKPGRFAVRGLPDRAATLAQIASAAHAAKALPPGLEPGLEASTIWDPENFTYP
                     SGTHLAFVEVDRATGEIRLLRVVAVDDAGRVINPMLVDGQIHGGLAQGIGQALCEHTV
                     YDEDGQCLTASLMDYAAPRADNLPPFDLDRVETPTPVNPLGAKGCGEAGTIGSTPAVV
                     NAVIDALAPLGVTHLDMPLTPARVWAAIDAANHRRTT"
     gene            2985..4172
                     /locus_tag="VLF19_05835"
     CDS             2985..4172
                     /locus_tag="VLF19_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92806.1"
                     /translation="MLTAPALAQEAVRIGFLTVRSGPLAAGGRQLEEGFQLFLKERNN
                     TVAGRKVEVVWADTGGQPALAKTKTQELVERNHVHVIIGPLAAFEALAINDYIKQVEV
                     PIISSSAAAEDLTQRKLNPWFVRAVVTSAQVTHPLGEYAAKVLGYKRVATIADDFAYG
                     HEGTSGFQRVFEDNGGKVVQKLWPPLNVADYGSYIAQIKGDVDAVYAGFAGGNGLRFL
                     KQYSEYGLKAKIPVIGNMTLTDAGILKSMGDEALGVITGAWYAAGLDTPANRRFVDAV
                     RQEYKADPGYYTAGAYIAGLFVEGAMKSVNGKIEDKAGFMKALRGLRLTETPIGPMRL
                     DEYGNPVLNIFIVKVERKDGRLVNSVIHSYPETSQFWKYPAKAFLASPVYSRDTPPAR
                     NIE"
     gene            4203..5078
                     /locus_tag="VLF19_05840"
     CDS             4203..5078
                     /locus_tag="VLF19_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92807.1"
                     /translation="MGFWLIQSLNGLALGGLLFLLAAGFSLIFGLMRVANLTHGAYFM
                     LGAYVGLTVVTAGLGFAAAVAAGALAVAVAGGLVERLVLRRLAARPPAQRALSQVLVT
                     LGLAFIVADGCLWLWGGDPMPLPVPAPLGGAVRVGGLAFPFYRLVVVAFSLVVALGLW
                     LGLERTRLGAMIRAGVDDMEMASGMGLPVSRLFTAVFAVGAALAGMGGVLGGPILSVY
                     PGLDTDMLPLALVVVILGGIGSLAGAFVGSYVIGFVYTFGQALLPDLAYVILFLPMVV
                     VLVVRPGGLFGTRGA"
     gene            5075..6004
                     /locus_tag="VLF19_05845"
     CDS             5075..6004
                     /locus_tag="VLF19_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE92808.1"
                     /translation="MKGWVVTGAALLAVPAIPSPYYVNIASQVLIAAIFALSLDLLVG
                     HAGLTSLGHAAYLGVAAYAVAWLTTRAGLDHLPAALAALALTLVMAAFFGVLALRGTG
                     LAFLMITLALGQILWGVAYRWVSLTGGDNGITGLTRPAPFGVDLARPGAFYLFTLAIA
                     ALAFAAVAVFVRSPLGACLEGTRDQPRRMQALGHHVWLVRWIAFVLAGVVAGVAGVLF
                     VYYNQFVGPQALSLTGSAEVLLMVISGGAGTLAGPVVGAAVVIVLKNVVSAHLTRWVM
                     LLGAVFVLIVVFMPDGLVPGIRRLWRTRSRSTA"
     gene            5974..6714
                     /locus_tag="VLF19_05850"
     CDS             5974..6714
                     /locus_tag="VLF19_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92809.1"
                     /translation="MANALEIDGLRKAFGGLPAINGVSLAVAEGERRLLLGPNGAGKT
                     TLFNLVTGDLVPDGGRVRVFGRDVTGARPDRCAHLGLGRTYQIITLFPHSTLVQNVVL
                     ALLGVSPRRWDPLTPLARRADLQSRARTVLARVGLAAIADKRVLHAAYGEQRRLEIAM
                     ALAQAPRLLLLDEPFAGLSREERRDMQAVLAAIPRQVTVLMIEHDMDVALSFAERITV
                     LHYGQVIVEGTRADIVASPRVKEIYLGE"
     gene            6707..7417
                     /locus_tag="VLF19_05855"
     CDS             6707..7417
                     /locus_tag="VLF19_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92810.1"
                     /translation="MSEALALSGVNTYYGDAHVLHDLTFALRPRRLLGLLGRNGAGKT
                     TAIRSIIGFTPPRTGEIHLFGEPIARLPPEAVAARGVGLVPQGRRVFATLTVQENLTV
                     VARRRADGPRWQLDRVFAVFPRLRERQHQLAGTLSGGEQQMLAIGRALMTNPRLLLLD
                     EPSEGLAPQLVAEVKRVLVALKAEDLSIVLVEQNTRLALDIADDVVILNSGVTAYDGP
                     PDAIRDDEAFLTLHLGVH"
BASE COUNT          961 a         2723 c         2608 g         1127 t
ORIGIN      
        1 ggtgcaacac gtcagagcag gccggcgagg gcttcggccg tttccttgcc cgcgaccacc
       61 ggaacgatct cgaagtgaac gagctcggac cactcggtga cccagcgttg caggagcgtg
      121 acgtcgtcgc actccatcag ctggaagcac cggccgaggt ccgccgcgac ccagctactc
      181 acgaacttca agccgtcggg catcatgcgg cccctgtcgc ggagcctccg gtagacggac
      241 ctcgcatcct ggttgcggaa ggtctcgacg accatgaaaa gcatcggcgc tcctcgctca
      301 gctcgctggt ggcgaagata gcccgccgcg gcgatccgag caatgctttg actggctccg
      361 gccgccgtga aatagtgtcc gcgccttgag cccggctcgc cggtccccgg atctcgcgtg
      421 agcgtcaccc gtctgctcgg ggccgcggtc cgccgccgcg aggaccccgc gctcgtccag
      481 ggggcggggc gctacgtgga cgacctggcg ccgccgcgcg cggtggcgct cgcgctcgtg
      541 cgctcgcccc acgcccacgc gcgtgtccgc gccatcgaca cgcgcgcggc cgccgcgatg
      601 cccggagtgc tggcggtgct caccgcgcgc gatctcgagg gccgtgtgag cccggagccc
      661 gccgtcggca tcccgcccaa cgcgcggcga ccgccacgcc cgctgctcgc cagcggcacc
      721 gtccgctacg tgggagaacc ggtcgtcgcc gtggtggccg agagccggta cgtggcggcc
      781 gacgggggcg aggccgtcct cgtggactac gagccgttgc cggccgtcgg cgaccccgag
      841 gccgcggcga cgcccggcgc cccgcgcgtg cacgaggagt tcgccgacaa cgtcgccctt
      901 cggtggtcgt ggacccacgg cgacgtggac gcggccttcg cccgcgcgcg ccacgtcgtg
      961 cgcctccggc tcgtgaacca gcgggtcgcc gggctcgcca tggagccgcg cggctgcgtc
     1021 gccgagtacc gtcccggcgc gctcaccgtg tgggccggca cccaggtgcc gcaccggctg
     1081 cgctcgggtc tcgccgagat gttgcggttg ccggagacgg ccgtgcgcgt gatcgcgccg
     1141 aacatcggcg gtggcttcgg ctgcaagatc ggctactacg tcgacgaggt cctttgcgcg
     1201 ctggcggcga tgaggctcga gcgcccggtc aagctcgtcc tcacgcggcg cgaggacttc
     1261 ctcacgacga ctcaggggcg cgggcagatc aacgatgtcg aggccgccgt ggccagggac
     1321 ggcacggtgc tcgccctgcg ctgccgcacg ctcgccgacc tcggagccta cctcgaagcg
     1381 ctgacgccct accccggcat gctcacgggc cggctcatca ccgggcccta tcgcatcccg
     1441 gccgccgcgt acgagctgac gggcgtcttc accaacgcga tggcgacggc gccgtaccgc
     1501 ggggccgggc gccccgaggc gacctacgcg ctcgagcgga cgatggacga gatcgcgcgc
     1561 gtcaccgggc tcgatgccgc cgaggtgcgc cgccggaact tgatccggcc cgacgagttc
     1621 ccctaccgcg cgccgtcggg cctcgtctac gactccgggc agtacgaggt cgccctcgac
     1681 cgcgcgctgg cgctggcgga ctacaagcgc ctgcgcgagg agcagcggcg cgcgcgcgag
     1741 gagggtcgcc tcgtcggcat cgggctctcg tcgttcatgg agaccgccgg cgtcggtccc
     1801 tccaggatcg cgccgatgcc cggctgggag agcgggagtg tccgcgtgga gccgtccggc
     1861 ctggtgacgg tcctgacggg gacgagcccg cacggccaag gcctcgagac cgccttcgcc
     1921 caggtcgccg ccgaccgtct cggcgtgccc atcgacgacg tcgcggtgct gcacagcgac
     1981 accgccgtcg tgcccgccgg cgtcggcacg ttcggcagcc ggtcgattgc cgtcggcggc
     2041 cccgccatcg ccgtctcggt ggacaagatc ctcgacaagg cgcggcgcat cgcggcggcg
     2101 ctgctcgagg cggcgccgga ggacctgatc tggaagccgg ggcgattcgc ggtgcgcggc
     2161 ctgcccgacc gcgcggcgac cctggcccag atcgcctccg ccgcccacgc ggcgaaggcg
     2221 ttgccgccag gcctggagcc ggggctcgag gcgagcacga tctgggatcc cgagaacttc
     2281 acgtatccgt cgggcacgca cttggcgttc gtggaggtcg atcgcgcgac cggcgagatc
     2341 aggctcctcc gcgtcgtcgc cgtggacgac gccgggcgcg tgatcaaccc gatgctcgtc
     2401 gacggccaga tccatggcgg gctcgcgcag ggcatcggtc aggcgctctg cgagcacacc
     2461 gtctacgacg aggacggcca gtgcctgacc gcctcgctca tggactacgc ggccccgcgc
     2521 gccgacaacc tgccgccctt cgacctcgac cgcgtggaga cgccgacgcc ggtcaacccg
     2581 ctgggcgcga agggctgcgg cgaggctggc accatcgggt cgacaccggc cgtcgtcaat
     2641 gctgtcatcg acgcgctggc cccgctcggc gtcacgcatc tcgacatgcc gctcacgccg
     2701 gcgcgcgtct gggcggccat cgacgccgcg aaccatcgga ggaccacatg aggaattcga
     2761 gcgtatcggg ataccgggag gtggagaagc gccacggcct cgccgaggcc cgtcgagcgt
     2821 cgggggcctg ggggccatct cggggccccc atgtcaccga ctccccccgg cgagtcttcg
     2881 aggtgcgcca cggctgtgcc ggggcgtgcc gagccgcccg gggggcttgg gggccatctc
     2941 ggggccccca tgtagttggt ctggtcgccg tcgcggcgct cctgatgctg accgcgccgg
     3001 ccctggccca ggaggcggtg cgcatcggct tcctcaccgt ccgctcgggc ccgctcgcgg
     3061 ccgggggtcg ccagctcgag gaagggtttc agctcttcct gaaggaacgc aacaacacgg
     3121 tcgcggggcg caaggtcgag gtggtctggg ccgacaccgg gggtcagccg gcgctggcga
     3181 agaccaagac ccaggagctg gtcgagcgca accacgtgca cgtcatcatc ggtccgctgg
     3241 cggccttcga ggcactggcg atcaacgact acatcaagca ggtcgaggtg ccgatcatct
     3301 cgtcgtcggc cgcggccgag gacctcaccc agcgaaagct caacccgtgg ttcgtccgcg
     3361 ccgtcgtcac ctcggcgcag gtgacgcatc cgctcggcga gtacgcggcc aaggtgctcg
     3421 gctacaagcg cgtggccacc atcgccgatg acttcgccta cggtcacgag ggcacctccg
     3481 gattccagcg cgtgttcgag gacaacggcg gcaaggtcgt gcagaagctc tggcctcccc
     3541 tcaacgtcgc cgactacggc tcgtacatcg cgcagatcaa gggcgacgtg gacgccgtgt
     3601 acgccggctt cgccggcggc aacgggctca ggttcctcaa gcagtattcg gagtacggac
     3661 tgaaggcgaa gatcccggtg atcggcaaca tgacgctgac ggacgccggc atcctcaagt
     3721 ccatgggcga cgaggcgctc ggcgtgatca ccggcgcctg gtacgccgcc ggcctcgaca
     3781 cgccggccaa tcgaaggttc gtggacgccg tccgtcagga gtacaaggcc gaccccggct
     3841 actacacggc gggcgcctac atcgccgggc tgttcgtcga gggcgccatg aagtcggtga
     3901 acgggaagat cgaggacaag gcggggttca tgaaggcgct gcgcggcctc cgtctcaccg
     3961 agacgccgat cggtccgatg cgcctggacg agtacggcaa ccccgtgctg aacatcttca
     4021 tcgtgaaggt cgagcgcaag gacggccggc tcgtcaacag cgtgatccac tcgtacccgg
     4081 agacgagcca gttctggaag tatcccgcca aggccttcct ggccagcccg gtgtactcgc
     4141 gcgacacgcc gccggcgagg aacatcgagt agcggcgcgc cgggagagga gaccgtccgg
     4201 cgatggggtt ctggctgatc cagagcctga acgggctcgc gctgggtggt ctcctcttcc
     4261 tgctcgctgc gggcttctct ctgatcttcg gcctcatgcg cgtggcgaac ctcacccacg
     4321 gggcctactt catgctgggc gcgtacgtgg ggctcaccgt cgtgaccgcg ggtctgggct
     4381 tcgcggccgc cgtggccgcc ggcgcgctcg ccgtcgcggt ggcgggcggc ctcgtcgagc
     4441 gcctcgtgct gcggcggctc gcggcgcggc cgccggccca gcgcgcactc tcccaggtac
     4501 tggtcactct cggcctcgcc ttcatcgtcg ccgacggatg cctctggctc tggggcggcg
     4561 acccgatgcc cctgccggtg ccggcgccgc tcggcggcgc ggtccgggtg ggcggcctgg
     4621 cgtttccctt ctaccgcctc gtggtcgtgg cgttctcgct cgtcgtggcc ctcggcctct
     4681 ggctcggcct cgagcgcacg cgactcggcg cgatgatccg cgccggcgtg gacgacatgg
     4741 agatggcgag cggcatgggg ctgccggtct cgcggctctt caccgcggtg ttcgccgtgg
     4801 gcgccgccct ggccggcatg ggaggcgtgc tcggtgggcc gatcctctcc gtctaccccg
     4861 ggctcgacac cgacatgctg ccgctcgcgc tcgtggtggt gatcctcggc ggcatcggca
     4921 gcctcgccgg cgccttcgtg ggcagctacg tcatcggctt cgtgtatacg ttcggtcagg
     4981 cgctgctgcc cgacctcgcg tacgtgatcc tcttcctgcc gatggtcgtc gtcctcgtcg
     5041 tgcggccggg cgggctcttc ggcacgcggg gcgcatgaag ggctgggtgg tgaccggtgc
     5101 cgcgctgctg gccgtgcccg cgatcccctc gccgtactac gtcaacatcg cgagccaggt
     5161 gctcatcgcc gcgatcttcg ccctcagcct cgacctgctc gtcggccacg ccgggctgac
     5221 ctcgctcggg cacgcggcat acctcggcgt cgcggcctat gcggtcgcct ggctcaccac
     5281 gcgcgccggc ctcgaccatc tgccggccgc gctggccgcc ctggcgctca cgctggtcat
     5341 ggcggcgttc ttcggcgtgc tcgccctgcg gggcaccgga ctggccttcc tgatgatcac
     5401 cctcgcgctc gggcagatcc tgtggggcgt cgcctaccgc tgggtctcgc tcaccggcgg
     5461 cgacaacgga atcacggggc tcacgcggcc cgctccgttc ggtgtcgacc tcgcccggcc
     5521 cggcgccttc tacctcttca cgctcgccat cgcggccctc gccttcgccg ccgtcgccgt
     5581 gttcgtgcgc tcaccgctcg gcgcctgcct cgagggcacg cgcgaccagc cgagacggat
     5641 gcaggcgctc ggccaccacg tctggctcgt ccgctggatc gccttcgtcc tggccggcgt
     5701 ggtggcgggc gtcgcgggcg tgctcttcgt ctactacaac cagttcgtcg gcccccaggc
     5761 cctgagcctg accggctcgg ccgaggtcct gctgatggtg atctctggcg gcgccggcac
     5821 gctcgccggc cccgtcgtcg gcgcggccgt ggtcatcgtc ctcaagaacg tcgtcagcgc
     5881 ccacctgacg cgctgggtga tgctgctcgg ggccgtcttc gtcctcatcg tcgtcttcat
     5941 gcccgacggg ctcgtgcccg ggatccggag attgtggcga acgcgctcga gatcgacggc
     6001 ctgaggaagg cgttcggcgg gctgccggcc atcaacggcg tgagcctcgc cgtcgccgag
     6061 ggcgaacgcc ggctgctgct cggccccaac ggcgccggca agacgacgct cttcaatctc
     6121 gtcacggggg acctcgtgcc cgacgggggc cgcgtgcgcg tgttcggccg cgacgtgacg
     6181 ggcgcgcggc ccgaccgctg cgcgcacctc ggactggggc gcacgtacca gatcatcacg
     6241 ctcttcccgc acagcaccct cgtgcagaac gtggtgctgg cgctgctcgg tgtctcgccg
     6301 cgtcgctggg atccgctgac gccgctggcg cggcgcgccg acctccagag ccgggcgcgc
     6361 accgtgctcg cgcgcgtggg cctcgcggcg atcgccgaca agcgggtcct gcacgccgcg
     6421 tacggcgagc agcgacgtct ggagatcgcg atggcgctgg cgcaggcgcc gcgcctgctg
     6481 ctgctcgacg agcccttcgc cggcctctcg cgcgaggagc ggcgcgacat gcaggccgtg
     6541 ctggcggcca tcccgcgcca ggtcacggtg ctgatgatcg agcacgacat ggacgtggcg
     6601 ctgtccttcg ccgaacggat cacggtgctg cactacgggc aggtgatcgt ggagggcacg
     6661 cgcgccgaca tcgtggcgag cccacgcgtc aaggagatct acctcggtga gtgaggcgct
     6721 cgcgctcagc ggtgtcaaca cctactacgg cgacgcccac gtgctgcacg atctgacctt
     6781 cgccctccgc ccgcggcggc tgctcggcct gctcggacgc aacggcgccg gaaagaccac
     6841 ggctatccgc tcgatcatcg gcttcacgcc gccccgcacc ggtgagatcc acctcttcgg
     6901 cgagccgatc gcgcggctgc cacccgaggc ggtcgccgcc cggggcgtgg gactcgtccc
     6961 ccagggccgc cgcgtgttcg ccacgctgac cgtgcaggaa aacctgaccg tcgtcgcccg
     7021 ccgccgggcg gacggcccgc gctggcagct cgaccgggta ttcgccgtgt ttccgagact
     7081 gcgcgagcgc cagcatcagc tggcgggcac gctgtcgggc ggcgaacagc agatgcttgc
     7141 gatcggccgc gcgctgatga ccaacccgcg cctgctgctg ctcgacgaac cgtccgaggg
     7201 cctcgcgccg cagctcgtgg ccgaagtcaa gcgcgtgctc gtcgccctca aggccgagga
     7261 tctttcgatc gtgctcgtcg agcagaacac gcggctggcg ctcgacatcg ccgacgacgt
     7321 cgtgatcctg aacagcggcg tgacggccta cgacgggccg ccggacgcca tccgcgacga
     7381 cgaggcgttc ctcacgctcc atctcggcgt ccactagtc
//
LOCUS       DATIAZ010000168        10558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_833136, whole genome shotgun sequence.
ACCESSION   DATIAZ010000168 DATIAZ010000000
VERSION     DATIAZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833136"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..717)
                     /locus_tag="VLF19_05860"
     CDS             complement(136..717)
                     /locus_tag="VLF19_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92811.1"
                     /translation="MAPPVDPNDVLMTGENSFIRLSRDGGKTPTDRLSHWRVLWCPSG
                     TGHALFIQSELTDGRVRTYSDNVAVARWLQRTIETLLFPAFADTTLPVIAAEFSRAGD
                     PRSTAVETIASAADVIRMTWYDCIAPFVLNAPPGFNNRPIGVFSTFFPARGAQVELNG
                     RFASGQPWAEQRGDRQASSACLAWSETWVKPPA"
     gene            1302..1628
                     /locus_tag="VLF19_05865"
     CDS             1302..1628
                     /locus_tag="VLF19_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92812.1"
                     /translation="MATANQLERLRAHVTTTVEHATDLLADLTSIDELFTVLLPSLTA
                     LTDDEREALVARLCGYDAALTGRLRTLVESLADVLAGLMTADGGRAWVDHHVARIEAG
                     EDAEAA"
     gene            complement(1632..2312)
                     /locus_tag="VLF19_05870"
     CDS             complement(1632..2312)
                     /locus_tag="VLF19_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSE92813.1"
                     /translation="MSDALLETVEVETGPAPRASVVWMHGLGADGHDFEPIVPALGFG
                     AAPAVRFVFPHAPMRPVTINNGWVMRAWYDVRNDGGERREDEPGVRASQQAIEALIAR
                     ETARGFHASRIVLAGFSQGGAMALHTGLRHPERLAGILALSCFLPLASTLEGEASAAN
                     RAVPIIMAHGVHDQLIPLARARQSRHVLSALGWNVEWHEYPVAHAVCDEEVADVAAWL
                     RRALGAGA"
     gene            complement(2309..2752)
                     /locus_tag="VLF19_05875"
     CDS             complement(2309..2752)
                     /locus_tag="VLF19_05875"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HSE92814.1"
                     /translation="MEPLLAYFHVLAFVLTGAFLTTELVLCRPGLAAPHVRLLPRLDI
                     VFFLSALVALATGLLRLFFYAKGVAFYLPNPAFHAKMALYLAVAVLSVYPTLRFIRWR
                     RALIEGDALPPSQEVAAVRRLLHIELGLFALMPLMAVLMARGIGR"
     gene            complement(2754..2888)
                     /locus_tag="VLF19_05880"
     CDS             complement(2754..2888)
                     /locus_tag="VLF19_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92815.1"
                     /translation="MPWCDDLVTVKPEIADDHLIAPRRPRWGTDVDEDPVRAHLPRRR
                     "
     gene            complement(2893..4308)
                     /locus_tag="VLF19_05885"
     CDS             complement(2893..4308)
                     /locus_tag="VLF19_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSE92816.1"
                     /translation="MSGAVCDLDLRGVSETIARGEVTSVQATEAYLDRIERHDRVLHA
                     YVTVMADEALARAKAADAEQAHGRRRGPLHGVPIALKDLIAVAGVRMTAGSRVLADHI
                     PARDACVTERLHAAGAVVLGKLSMHEFAFGRPATEGPFPTGRNPWDVHREPAGSSSGS
                     GVAVAAGLCAGALGSDTGGSIRGPAARCGIVGLKPTYGLVSRRGVVAMDWSLDHVGPM
                     TRTVWDTAVLLQAIAGYDAKDASTRVAPMPTPAFTSLLEAGVRGMRLGVLRRFYVDWP
                     GLDGEVRAASLAAMAELAHQGATLEDVDAPTLDLAPAIWATFLAEMYEYHRETFSARP
                     HDYREGTRIRLMMGALVTAHDFLRGQRLRARLAREVRALLERVDALVFPGHAAPAQRF
                     EEVSTREVMAPGGRYTSPWNLLGLPAIVVPCGFSREGLPLALQIVGRPFDEPTVLRVA
                     RAYERATDWHDRRPDPAGWQG"
     gene            complement(4305..4478)
                     /locus_tag="VLF19_05890"
     CDS             complement(4305..4478)
                     /locus_tag="VLF19_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92817.1"
                     /translation="MADDAALRARFERELEVADLAPAGRDRELLYAMWVEHLPQREAL
                     RAAAPALDEEPLP"
     gene            4610..5278
                     /locus_tag="VLF19_05895"
     CDS             4610..5278
                     /locus_tag="VLF19_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HSE92818.1"
                     /translation="MRATSHASSRNGARRRSTVVLLVRHAATATTGVRLPGRSAGLHL
                     SDEGRRQADAVAARLGRLRRVAAVYTSPLERARETAVAIARALGTTPRVDAGLLEVDI
                     GDWTGLSIARASRRAEWQTVQRHPSGFRFPGGESFMELQARIVETVARLAARHPGAIV
                     VAVSHADPIKAAVAHALGAHLDLFQRVVVAPASVTAIAYRSTGPAVLTVNSVDGDLAG
                     LGLE"
     gene            5275..5784
                     /locus_tag="VLF19_05900"
     CDS             5275..5784
                     /locus_tag="VLF19_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HSE92819.1"
                     /translation="MSASFDLDAPDHFTTGTVGQPGQRVFYLQGRQSRKMVTLKCEKE
                     QVAALGEYLAGLLAQLSPAGSSAPKETDLVEPVEASWAVGSIGVGYDREADRIVVEAT
                     ELVDEEERETEAATARFRISRGQAATFVERARALIKGGRPSCPMCGQAVDPGGHFCPR
                     ANGHQTRRS"
     gene            5795..6499
                     /locus_tag="VLF19_05905"
     CDS             5795..6499
                     /locus_tag="VLF19_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HSE92820.1"
                     /translation="MPATEEALALLARGAVTIRGRLPGASNATFLVDVAHGDEQTFAV
                     YKPGRGERPLWDFPPGLYRREVAAFLLSEALGWGLVPPTVLRDGPYGEGSLQYFVDAD
                     FEQHYFTLHEHPAHRDRLARICVFDLLANNADRKAGHCLLGDDGAIYAIDNGLTFHVG
                     PKLRTVIWDFGGEPIPPPVLADVARLLDAGFAPALGALLAPAECAALLARARALVAAA
                     SFPAEPGGRCYPWPLV"
     gene            6593..7036
                     /locus_tag="VLF19_05910"
     CDS             6593..7036
                     /locus_tag="VLF19_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE92821.1"
                     /translation="MIQARKLGHVVLKVRDAAKSRDFYTRTLGLKVSHENLEHGAVFL
                     SFGQEHHELALFQMATDEAPKGPQAGLHHMAWRLGSFEELREAYKELKALGVPVEATT
                     EHNVSRSVYFPDPDGNRVELYCDMFENGFEAMRTLGPKREPLDLE"
     gene            complement(7083..8147)
                     /locus_tag="VLF19_05915"
     CDS             complement(7083..8147)
                     /locus_tag="VLF19_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HSE92822.1"
                     /translation="MEIPGSLRVPMETPDGAGHAGVSRLLGRMRRSAFQGRKLGEAFE
                     AWGRMIDGGSLICLGLAGSMASAGLGPLVAWLVERGYVDVVVSTSANVTEDLLEQRGA
                     RFWQIDPDHVDDEALWRQGFYRFYDHVVGSAEYDAMEDFTAGFFEHLAATWRGPAIPA
                     VRLMHEFGRWLDAGGLGRTVAATCYRHRVPLFVPAAPDGPLSEGYRVARRKGPVVDFF
                     KDYEVALGMMERWMRPGRGTSAIFLGGGVPKDFIQITATSVSAIRGGGEPSPHLAAIQ
                     VTTDNTVFGGLGGAGVNTECISWGKESPAGDNVMLFTDVTIALPLLCQGLAEHYGAAH
                     VRPARATHAAELGSILDACA"
     gene            8244..9071
                     /locus_tag="VLF19_05920"
     CDS             8244..9071
                     /locus_tag="VLF19_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE92823.1"
                     /translation="MATPAVAQLFDAMADDYDTLEPWYGHYYPVLHALLRSALAPSNG
                     APPGRVLDAGCGTGFQAALLESMGYVTHGLDLSSRLLAAAARRLRAPALARGNVESLP
                     YRDEAFDAVTCCGSTLSFVERPATAVAEIARVLRPGGRLFLDCEHKWSVDALWTLASA
                     LAGDPLGYGLTAVEAWRALARRGGCVVPYPGYGALRLFTLAELRAMLRHAGLEPVRTW
                     GIHATTPLIPSTVLHRRRLPGPVATLFRGLCALDDAVRRLPGSARVASSVVLLARRV"
     gene            complement(9245..9799)
                     /locus_tag="VLF19_05925"
     CDS             complement(9245..9799)
                     /locus_tag="VLF19_05925"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HSE92824.1"
                     /translation="MATDMILWRRLDVPGHDACRLEQTAGGWRLNGAAIFRHEDGPAC
                     LAYQVVCDGGWRTQEGVVHGWMGSRPLDLRTVRRPDGVWTLNGQIVPDLDGCVDLDLG
                     FTPATNLLQLRREALHVGQRADVSVAWLDASAGTLDVLHQRYERRDGRLYRYEAPRFD
                     YVALLEVNEVGFIEKYPHLWEAEP"
     gene            complement(10259..>10558)
                     /locus_tag="VLF19_05930"
     CDS             complement(10259..>10558)
                     /locus_tag="VLF19_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017610005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92825.1"
                     /translation="NVPVYRAFHEWLGRTPELGPMWEAWTRGDRKGAVAAIPGRVVDE
                     LVLRGSVDEIRARVRRYFEAGLDTAFLAFQTSESDRDRKREVVRGAMRALAPTRG"
BASE COUNT         1525 a         3808 c         3816 g         1409 t
ORIGIN      
        1 gaggatcgcg ctctccggcg gcaagtgtca agggtgagct cggctccagg aatgcggcga
       61 gggtcgcgaa gtcttcgcca aggtcggcga gaggatggcg tcggccgcgg gttaagccgg
      121 cgccggcggc cccgctcacg ccggcggctt cacccacgtc tccgaccacg ccaggcaggc
      181 gctggaggcc tgccggtcgc cgcgctgctc cgcccagggc tgcccggacg cgaaccggcc
      241 gttcagctcc acctgcgcgc cgcgcgccgg gaagaacgtg ctgaacacgc cgatggggcg
      301 gttgttgaag ccgggcggcg cgttgagcac gaagggcgcg atgcagtcgt accacgtcat
      361 gcggatcacg tcggcggcgg aggcgatcgt ctcgaccgcg gtcgagcgcg gatcgcccgc
      421 gcgcgagaac tcggcggcga tcacgggcag cgtcgtgtcg gcgaaggccg gaaagagcag
      481 ggtctcgatc gtccgctgca gccagcgcgc caccgcgacg ttgtcgctgt aggtccgcac
      541 gcgcccgtcg gtgagctcgc tctggatgaa gagcgcgtgg ccggtgccgg atggacacca
      601 gagcacccgc cagtggctca ggcggtcggt cggggtcttg ccgccgtcgc ggctcagacg
      661 aatgaacgag ttttcgccgg tcatcaggac gtcgttcgga tcgacgggtg gcgccatgcg
      721 cgctcctccg ggctgacggg atgacgtgcg ggctccgtga gccccgggag actctactac
      781 ggcccgggct cggagcgaat gcgcggcagg gagaacggcc tttagcgggc ccgatcggga
      841 cgcacggcgt cgccgggcac agggagctcg ctcgagagct cttcccgagc gggagccaga
      901 acacggcacc gcccgggcag gcggacgccg aggtcgcccg gcaccggccc ggactcacac
      961 gagaccagcc cctgctcgta gacaggcagg ctcacgcgct ccagccccac cgcggcgggc
     1021 ggttggccgg caggcggttg ggagatgggt ggttgagtga tgggtggttg ggcgacgggc
     1081 ggctgagtgg cgggcggcgc agatggcggt accacgggcg gtggcaggat ggggtggaga
     1141 agaacgtcgt cgaagaagac ctgacccggg aacggtcagc gggaagtgca gcgtgccgtc
     1201 ccctgtcgtg tcgtcgatgc tgacggagac ccccgcgcga gcctgatccg cgttgacgct
     1261 cctgagggtc gacggcatca tggttgcagc ggcgcggcat catggcgacg gcgaaccagc
     1321 tcgagcgcct gcgcgcgcac gtgacgacga ccgtcgagca cgccaccgac ctgctcgccg
     1381 acctcacctc gatcgacgag ctgttcaccg tcctgctccc ttccctcacc gcgctgaccg
     1441 atgacgagcg cgaagcgctg gtggcgcgcc tctgcggcta cgacgccgcc ttgaccgggc
     1501 ggctgcgcac gctggtggag agcctcgccg acgtgctggc cgggctcatg acggcggacg
     1561 gcggtcgggc ctgggtcgac catcacgtgg cacggatcga ggccggcgag gacgccgaag
     1621 cggcctgagc gctacgcgcc ggcgccgagc gcccggcgca gccaggcggc gacgtcggcg
     1681 acctcctcgt cgcagaccgc gtgcgccacc gggtactcgt gccactcgac gttccagccg
     1741 agcgcgctca gcacgtggcg cgactgccgc gcgcgcgcca gggggatgag ctggtcgtgg
     1801 acgccgtggg ccatgatgat cggcaccgcg cgattggcag cgctcgcctc gccttcgagc
     1861 gtggacgcga gcggcaggaa acacgacagc gcgaggatgc cggccaggcg ctcgggatgg
     1921 cgcaggccgg tgtggagcgc catcgccccg ccctgcgaga agccggcgag cacgatgcgc
     1981 gaggcatgga agccgcgcgc cgtctcccgc gcgatcagcg cctcgatcgc ctgctgcgag
     2041 gcccgcacgc ccggctcgtc ctcgcgccgc tcgccgccgt cgttgcggac gtcgtaccag
     2101 gcgcgcatca cccagccgtt gttgatggtg accggccgca tcggcgcgtg cgggaacacg
     2161 aagcgcaccg ccggcgcggc cccgaacccg agcgcgggca cgatgggctc gaagtcgtgg
     2221 ccgtcggccc cgaggccgtg catccagacc acgctcgcgc gcggcgcggg accggtctcg
     2281 acctcgacgg tctcgagcag ggcgtcgctc accgcccgat cccccgcgcc atgagcacgg
     2341 ccatgagcgg catcagcgcg aacaggccga gctcgatgtg cagcagccgg cgcaccgccg
     2401 ccacctcctg gctgggcggc agcgcgtcgc cctcgatcag cgcgcgccgc cagcgaatga
     2461 accggagcgt cgggtagacc gagagcaccg ccaccgcgag gtagagcgcc atcttcgcgt
     2521 ggaacgcggg gttcggcagg tagaaggcga ctcccttggc gtagaagaag agccgcagga
     2581 gccctgtcgc gagcgcgacg agcgcggaca ggaagaacac gatgtccagg cggggcagca
     2641 ggcggacgtg gggcgcggcg aggcccggcc ggcacagcac cagctccgtg gtcaggaacg
     2701 cgccggtcag gacgaacgcg aggacgtgga aataggcgag cagcggctcc atgtcagcgc
     2761 cgccgcggca ggtgcgcgcg cacggggtcc tcgtcgacgt ccgtccccca ccgcggccgg
     2821 cgcggcgcga tcaggtggtc gtcggcgatc tccggcttca ccgtcacgag gtcgtcgcac
     2881 cagggcaccg tgtcagccct gccagccggc gggatcgggc cggcggtcgt gccagtcggt
     2941 ggcgcgctcg taggcgcgcg cgacgcgcag cacggtcggc tcgtcgaacg gccgacccac
     3001 gatctggagc gcgagcggca ggccctcacg gctgaagccg cacggcacga cgatcgccgg
     3061 cagcccgagc aggttccacg ggctcgtgta tcgcccgccc ggcgccatca cctcgcgcgt
     3121 ggacacctcc tcgaagcgct gggcgggggc cgcgtggccg gggaagacca gcgcgtccac
     3181 gcgctcgagc agcgcgcgca cctcgcgggc caggcgcgcg cgcagccgct ggccgcgcag
     3241 gaagtcgtgg gcggtcacca gcgcgcccat catgagccgg atgcgggtgc cctcgcggta
     3301 gtcgtgcgga cgggcgctga acgtctcgcg gtggtactcg tacatctcgg ccaggaacgt
     3361 cgcccagatc gccggcgcga gatcgagcgt gggcgcgtcc acgtcctcga gcgtggcgcc
     3421 ctggtgcgca agctcggcca tggcggcgag cgacgctgcg cggacctcgc cgtcgagtcc
     3481 gggccagtcc acgtagaagc gccgcagcac gccgagcctc atcccgcgca cgccggcctc
     3541 gagcaggctc gtgaacgccg gcgtcggcat cggcgccacg cgtgtcgagg cgtccttcgc
     3601 gtcgtaaccg gcgatcgcct gcagtaacac cgcggtgtcc cagaccgtcc gggtcatcgg
     3661 gccgacgtga tcgagcgacc agtccatcgc caccacgcca cggcggctca cgaggccgta
     3721 cgtcggcttg agcccgacga tgccgcatcg cgcggccggc ccgcggatcg agccgcccgt
     3781 gtcggagccg agcgcgcccg cgcagaggcc cgcggccacg gcgacaccgg agcccgacga
     3841 tgagccggcc ggctcgcggt gaacgtccca gggattgcgg ccggtgggaa acggcccctc
     3901 ggtggccggg cggccgaagg cgaactcgtg catcgacagc ttgccgagga cgaccgcgcc
     3961 cgcggcatgc aggcgctcgg tcacgcaggc gtcacgcgcc gggatgtggt cggcgagcac
     4021 gcgcgagccg gccgtcatgc gcactccggc gacggcgatg aggtccttga gcgcgatcgg
     4081 cacgccgtgc agggggccgc gccgccgtcc gtgcgcctgc tcggcgtcgg cggccttggc
     4141 ccgcgccagc gcctcgtccg ccatcacggt gacgtaggcg tgcagcacgc ggtcgtgacg
     4201 ctcgatgcgg tcgaggtagg cttccgtcgc ctggaccgac gtcacctcgc cgcgcgcgat
     4261 cgtctcgctc acgccccgga gatcgaggtc gcagacggcg ccgctcacgg cagcggctcc
     4321 tcgtcgagcg cgggcgccgc cgcccgcagc gcctcgcgct gcggcagatg ctcgacccac
     4381 atcgcataga gcagctcgcg atcgcgtccg gccggggcga gatccgccac ctcaagctcc
     4441 ctctcgaagc gcgcgcgcaa cgccgcgtca tcggccatgc cgtggccctc cgtgcgcagc
     4501 acattacccc gaggtggcga gcgagggcgg cgcagccgcc ctaccggtgc tgccccggca
     4561 cgccgccgcg tacttgcgcg cgccggagag cgccgactat gctgccccgg tgcgcgcgac
     4621 atctcatgcg tcctcgagaa acggcgcccg gcgccggtcg accgtcgtcc tgctcgtccg
     4681 tcacgccgcg acggccacga ccggcgtgcg cctgcccggt cgctcggcgg gcctgcacct
     4741 gagcgacgag ggccggcgcc aggccgacgc ggtcgcggcc cggctcgggc ggctgcgccg
     4801 tgtcgccgcg gtctacacct cgccgctcga gcgtgcgcgg gagacggccg tcgccatcgc
     4861 gcgcgccctc ggcacgaccc cgcgcgtcga cgccgggctc ctcgaggtcg acatcgggga
     4921 ctggaccggc ctctcgatcg cgcgggcgag ccgccgcgcc gagtggcaga ccgtgcagcg
     4981 gcacccgagc ggcttccggt ttcccggcgg cgaatcgttc atggagctgc aagcgcgcat
     5041 cgtggagacg gtcgcgcgcc tggccgcccg gcatccgggc gcgatcgtcg tggccgtctc
     5101 ccacgccgac cccatcaagg ccgccgtggc ccacgcgctc ggcgctcatc tcgacctgtt
     5161 ccagcgcgtc gtcgtggcgc cggcctcggt gacggcgatc gcgtaccgat cgaccggacc
     5221 ggccgtgctg accgtcaact ccgtggacgg cgaccttgcc gggctggggc tcgaatgagc
     5281 gcctcgttcg acctcgacgc tcccgatcac ttcacgacgg gaacggtcgg ccagccgggc
     5341 cagcgagtct tctatctgca aggacggcaa tcgcgcaaga tggtgaccct caagtgcgag
     5401 aaggagcagg tcgccgcgct cggcgaatac ctcgcggggc tcctcgctca gctcagcccg
     5461 gcggggtcgt cggcgccgaa ggagaccgac ctcgtcgagc cggtcgaggc ctcctgggcc
     5521 gtcggctcca tcggcgtcgg ctacgaccgc gaggccgacc gcatcgtcgt cgaggcgacc
     5581 gagctggtgg acgaggagga gcgcgagacg gaggcggcca cggcacgctt ccgcatcagc
     5641 cggggccagg ccgccacctt cgtcgagcgg gcgcgcgcgc tcatcaaggg cgggaggccg
     5701 tcatgtccga tgtgcggcca ggccgtggac cccggcggcc acttctgccc gcgggccaac
     5761 ggtcatcaga cccgccggag ctgatggcgc cctgatgccg gccacggagg aggcgctcgc
     5821 actgctcgcg cgcggcgccg tcacgattcg cgggcgcctg ccgggtgcga gcaacgcgac
     5881 gttcctcgtg gacgtcgccc acggagacga gcagacgttc gccgtctaca agccggggcg
     5941 gggcgagcgg ccgctctggg acttcccgcc cggtctgtac cgccgcgagg tggcggcgtt
     6001 cctcctctcg gaggccctcg gctgggggct cgtgccgccg acggtgctcc gcgatggccc
     6061 ctacggcgag gggtcgctgc agtacttcgt ggacgccgat ttcgagcagc actacttcac
     6121 gctgcatgag cacccggcgc atcgcgaccg gctcgcgcgc atctgcgtct tcgacctgct
     6181 cgccaacaac gccgatcgca aggccggcca ctgcctgctc ggcgacgacg gcgcgatcta
     6241 cgcgatcgac aacgggctca ccttccacgt gggcccgaag ctgcgcaccg tcatctggga
     6301 cttcggcggc gagccgatcc cgccgcccgt gctcgccgac gtcgcgcgcc tgctcgacgc
     6361 cggcttcgcg ccggcgctgg gggcgctgct ggcgcctgcc gagtgtgccg ccctgctcgc
     6421 ccgcgcccgc gcgctcgtcg ccgcggcgag cttccccgcc gagcccggcg gccgctgcta
     6481 cccgtggccg ctggtgtagc gcgcgacgcg gcttgccggc ctgggccctc atccgctacg
     6541 atctccacgt cggtcaccga agcgacaagc gcgtgggcat gaggaggcgg acatgatcca
     6601 ggcacgcaag ctcggtcacg tcgtgctcaa ggttcgcgac gcggccaagt cccgggattt
     6661 ttacacacgg acgctgggac tgaaggtgtc ccatgagaat ctggagcacg gcgccgtgtt
     6721 cctcagcttc gggcaggagc accacgagct ggcgctgttc cagatggcga cggacgaggc
     6781 gccgaagggc ccgcaggccg gcttgcacca catggcctgg aggctcgggt ccttcgagga
     6841 gctccgcgag gcctacaagg agctcaaggc gctgggcgtg ccggtcgagg ccacgaccga
     6901 gcacaacgtg tcgcgcagcg tctacttccc cgaccccgac ggcaaccgcg tggagctgta
     6961 ctgcgacatg ttcgagaacg ggttcgaggc catgcgcacg ctcggcccca agcgggagcc
     7021 actcgacctc gagtaacgcc gcccgcgtgc ccttgaagcg gcatgcgatc gagaacggag
     7081 catcaagcgc acgcgtccag gatcgagccg agctcggcgg cgtgggtggc gcgcgcgggc
     7141 cggacgtggg cggcgccgta gtgctcggcg agcccctggc aaagcagcgg caacgcgatg
     7201 gtgacgtcgg tgaacagcat cacgttgtcg cccgccggtg attccttgcc ccacgagatg
     7261 cactcggtgt tgacgccggc gccgccgagg ccgccgaaga cggtgttgtc ggtcgtcacc
     7321 tggatcgccg ccaggtgcgg gctcggctcg ccgccgccgc ggatggcgga gacgctggtg
     7381 gcggtgatct ggatgaagtc cttcggcacg ccgccgccga ggaagatcgc cgaggtgcct
     7441 cgacccgggc gcatccagcg ctccatcatg cccagcgcga cctcgtagtc cttgaagaag
     7501 tccacgacgg ggcccttccg cctggccacg cggtagcctt ccgacagcgg cccgtccggc
     7561 gccgccggca cgaagagcgg gacacgatgg cgatagcagg tggcggcgac ggtccgcccc
     7621 aacccgccgg cgtcgagcca ccgcccgaac tcgtgcatga gccgcacggc ggggatcgcg
     7681 gggccacgcc acgtggcggc gaggtgctcg aagaagccgg ccgtgaaatc ctccatggcg
     7741 tcgtactccg ccgagccgac cacgtggtcg tagaagcggt agaacccctg gcgccagagg
     7801 gcctcgtcgt ccacgtggtc gggatcgatc tgccagaagc gcgctccccg ctgctccagg
     7861 aggtcctcgg tgacgttggc cgaggtcgag acgacgacgt ccacgtagcc gcgctccacg
     7921 agccaggcca cgagcggccc gaggcccgcg ctcgccatcg agcccgccag gcccaggcag
     7981 atcaggctcc cgccgtcgat catgcgcccc cacgcctcga aggcctctcc gagcttccgt
     8041 ccctggaacg ccgatcgccg catgcgtccg agcagccggc tcacaccggc gtggccggcg
     8101 ccgtcgggcg tctccatagg tacgcgcagg gagcctggga tctccattcg agtcctcgca
     8161 tgcgtgagat caggaacgat gccgcccgtc ccatttacac tgatcccggc tcggagtcga
     8221 gcgaggggtg gaaaggagcg cgcatggcga ccccggcggt cgcgcagctg ttcgacgcga
     8281 tggcggacga ctacgacacg ctcgaaccct ggtacggcca ctactacccc gtgctgcacg
     8341 cgctgctgcg ctcggcgctg gcgccctcga acggcgcccc gccgggccgg gtcctcgatg
     8401 ccgggtgcgg gaccgggttc caggcggcgc tcctcgagtc gatgggctac gtgactcacg
     8461 gcctcgatct gtcgtcgcgg ctgctggccg ccgcggcgcg ccggctccgg gccccggcgc
     8521 tcgcccgggg gaacgtcgag tcgcttccct atcgagacga agcgttcgac gccgtcacct
     8581 gctgcggcag cacgctgagc ttcgtcgagc gcccggccac cgcggtcgcc gagatcgccc
     8641 gcgtgctgcg gccgggcggc cggctcttcc tcgactgcga gcacaagtgg agcgtggacg
     8701 ccctgtggac gctggccagc gcgctggccg gcgaccctct cggctacgga ctcacggccg
     8761 tcgaggcgtg gcgggcgctc gcgcgccgcg gcggctgcgt ggtgccgtac cccggctacg
     8821 gggcactgcg gctgttcacg ctggccgagc tccgcgcgat gctgcggcac gccggcctcg
     8881 agccggtgcg cacctggggc atccacgcga cgacgcccct gatcccgtcc accgtgctgc
     8941 accggcgccg gctgcccgga cccgtcgcca cgctgttccg cgggctctgc gccctggacg
     9001 acgccgtgcg tcgcttgccg ggcagcgcgc gcgtcgcgag cagcgtcgtc ctcctcgcgc
     9061 gcagagtctg aggccgtcga ggacgcaaac gacctttctt gtccgacgcc tcagcccacc
     9121 gaccgcatga gcgcggcagc agtcatcggt agctcggaca ggttcgtgag gtggccgtcg
     9181 cccgggagag cgtggaaggt cgagccacat cccctaagaa cgcacggaag aagccggctg
     9241 ttgatcacgg ctcagcctcc cagaggtgcg gatacttctc tatgaagccg acttcgttga
     9301 cctcgagaag cgcgacgtag tcgaatcgtg gcgcctcgta ccggtacagt ctaccgtcgc
     9361 ggcgctcata tcgctgatgg agcacatcga gggtgcccgc agacgcgtcg agccatgcga
     9421 cggagacatc tgcgcgctgc ccgacgtgga gtgcctcgcg ccgtagctgg agcaggttcg
     9481 tagccggagt gaacccgaga tcaagatcga cgcacccatc aagatcgggc acgatctgtc
     9541 cgttcaacgt ccacacacca tcgggccttc gcacagtccg gaggtcgagg gggcgtgaac
     9601 ccatccagcc atgcaccaca ccctcctgcg tccgccatcc gccatcgcat acgacttgat
     9661 aggcgaggca tgccggtcca tcctcgtgcc ggaagatggc cgcgccattg agtcgccagc
     9721 cgccggctgt ctgttccagc cgacacgcgt cgtgaccggg gacgtccagg cgacgccaga
     9781 gaatcatgtc tgtcgccatc cagcgtcctt tatgggtccg cccaacgact attcgactgc
     9841 ggcgtcgtca tctgcgacga gatgacggca tccaacagtg gatgcgagca tctccatctg
     9901 gatggcatcg ggcatctgcg tcgagattct ctccccacgg ggagcgcctc ataccgcgca
     9961 agaggccgct ccgggcccgt cgggcctggc tcgagatgca gccgctgccg ggaggtggca
    10021 gatgcgggcg agcggcgggg tagcgtggcg cgggcggagg ccccgccggg cgcgatcgac
    10081 gaggcgcggc aaagggggcg gaggctcctg gaccgcggac gcggcgagaa cgctggtcac
    10141 gcgacggctc gcctctctca gccggcatgg tagacgacga gcccggatca cccgacgtcc
    10201 cgatccgatt cgtcgtccgg gacggaagga tggggggctc ctgccaggcg acgtgtggtc
    10261 agccgcgcgt cggcgccagc gcgcgcatcg ccccgcgcac cacctcgcgc ttgcggtcgc
    10321 ggtccgactc gctggtctgg aacgccagga acgcggtgtc gaggccggcc tcgaagtagc
    10381 ggcggacgcg cgcgcggatc tcgtccacgg agccgcgcag caccaactcg tcgaccacgc
    10441 ggccgggaat ggcggccacg gcgcccttcc ggtcgccgcg cgtccacgcc tcccacatcg
    10501 ggcccagctc gggcgtgcgc ccgagccact cgtggaatgc acggtacacg ggcacgtt
//
LOCUS       DATIAZ010000169        26351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_571242, whole genome shotgun sequence.
ACCESSION   DATIAZ010000169 DATIAZ010000000
VERSION     DATIAZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 26351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26351
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571242"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VLF19_05935"
     CDS             <1..336
                     /locus_tag="VLF19_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSE92826.1"
                     /translation="GLLMLLIFLLGWPLEWPAIIFIFLPIFIPVVVALKFDLIWFSAL
                     VALNLQTAFLSPPVAMAAYYLKAVVPSWELNEIYRGMVDFMLLQVVGLMLLFFFPQIA
                     LWLPSLLYK"
     gene            374..925
                     /locus_tag="VLF19_05940"
     CDS             374..925
                     /locus_tag="VLF19_05940"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HSE92827.1"
                     /translation="MSPGPEESTPPATPGALDRVITAIDAVSTVAGWVAGWLIVPMTL
                     AVSYEVAARYAFNAPTAWAYDTTYMLFGAQFMLAAAYTLLKGGHIRTDVFYERWSPQT
                     RATIDVVSYLFFFFPGMLFVLYAGGVEAWHSWQIGERSDWTPWRPILYPLKAVIPLTA
                     ALLVLQGFSELVKCLRVIRGRPR"
     gene            922..1962
                     /locus_tag="VLF19_05945"
                     /pseudo
     CDS             922..1962
                     /locus_tag="VLF19_05945"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            2078..2239
                     /locus_tag="VLF19_05950"
     CDS             2078..2239
                     /locus_tag="VLF19_05950"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92828.1"
                     /translation="MAAYYLKGVAPDWKLTDIYKGMGEFMVLQVIGLLVVVLYPPIAL
                     WFPEWLFGK"
     gene            complement(2243..3334)
                     /gene="ugpC"
                     /locus_tag="VLF19_05955"
     CDS             complement(2243..3334)
                     /gene="ugpC"
                     /locus_tag="VLF19_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HSE92829.1"
                     /translation="MAQVLMKDLNKKYDEVHAVKDVNLHIRDTEFIVLVGPSGCGKST
                     TLRMVAGLEEITAGEISIGDRVVNDLPPKDRDIAMVFQNYALYPHMTVYDNMAFGLKM
                     RKFPKTEIQKRVQDAAEILGIQELLKRKPRQLSGGQRQRVAVGRAIVRHPQVFLFDEP
                     LSNLDAKLRVQMRVELKRLHDRLETTAIYVTHDQVEAMTLGDRVVVMKDGWVQQVGEP
                     LELYSHPANRFVAGFIGSPSMNFVEVTPVDAGGSLWVECPGLRIKVPPALVPRLSPHK
                     GQRLTLGIRPEDLRVGGGGDLADQAFDAVVEVVEPLGSEILLDVKVGPSMLVARVEPT
                     VRVKVHETLRLAALPDRLHFFDPSNEKAL"
     gene            complement(3367..4218)
                     /locus_tag="VLF19_05960"
     CDS             complement(3367..4218)
                     /locus_tag="VLF19_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSE92830.1"
                     /translation="MVIERRWKRWVFFYVPLTVFVVGTLFPFYWMLVTAIRPDVELYR
                     SWRAVNNAPFWTVHPTLSHVQDLLARTTFPRWLWNTFFIAIVSTGISLFCGLLGGYAL
                     ARLRFPMAGVLGTSIFVTYLVPPTLLFIPLADIIRSFHLGNTPWALILTYPTFLIPFC
                     TWLLMGYFKTIPRELEECARIDGATRFGAMVRIIFPIAVPGILSAGIFAFTLSWNEFI
                     YALVFLSSPERKTVPVGVVSELIRGDIYFWGQLMAGALLGSVPVALVYSFFVEYYVTG
                     LTGSVKG"
     gene            complement(4219..5184)
                     /locus_tag="VLF19_05965"
     CDS             complement(4219..5184)
                     /locus_tag="VLF19_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSE92831.1"
                     /translation="MNRPIAGGSGRAAGVEVALPRTPAQRRPLARVLEDERALATLLL
                     APTVVLLGLFIAYPFVMGVWLSLSSTSVGNPGVFVGLTNFAKAWNDSIFQTAFRNTFF
                     YTFWATLFKLVLGMWLALLLNRHFRGKRLVRASMLLPFIIPTVLSTFAWRWMFDPTFS
                     VINWTLYKTGFIVTKLPFLSDGVWALWCAIVVNTWRGMPFFAITLLAGLQTINPDLHE
                     AAALDGANGWKRFWHVTWPLLKPVTIVVVVFSVIQTFSDFQLIYVLTGGGPANSTHLL
                     ATYAYQVGVATGLLGEGAAVSLFMLPVLFVVVWLQLRYLRRLEGA"
     gene            complement(5216..6553)
                     /locus_tag="VLF19_05970"
     CDS             complement(5216..6553)
                     /locus_tag="VLF19_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE92832.1"
                     /translation="MSEHDSNRRYFLKLSGAAAVVASTGIEGILAAGRAPAWAQGTKL
                     HWVRWNDFIPECDVHLKNEAVAAASKALGAEVVLETIGYNDLQPRITAAVQSGAGADI
                     FMLYYNWAHLYQNALVDVSDVATAVAKEQGGFYEVFNAAARVGGKWLGMPHSIVGNAI
                     AYRRSWFKEVGFDKFPETWDDLRKAATALKKKGKPYGQTLGHTFGDAPTFTYPLMWSF
                     GGAETDKSGKKVAINSKGTLDSVKYMQALWKDGCDEGGLAWDDSNNNRAFQAGEVCAT
                     LNGASIYIVAKRQKDKLKDDKGEPLFQDIDHAIFPLKGGAGVYPLYFANAHGVMKYSK
                     NQKLAKDFLTWLHKKENYEKWFQIAEGYSVAATKSWENHPMWGKIDKPLQIFRTAARE
                     TRIFGYAGPSTAKSTEVYSKYIITDMYAKAVQGTKAEDAVKWAEAELKKIYEA"
     gene            complement(6763..7995)
                     /locus_tag="VLF19_05975"
     CDS             complement(6763..7995)
                     /locus_tag="VLF19_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92833.1"
                     /translation="MRERSISYGWVVVGVGALITCVGMGALFSMGIFLKPIEESMGWS
                     RTGISTIALINWIAMGLGSLVWGALSDRYGGRVVVLGGGALLGLGLVLSSQVTALWQL
                     YVTFGVTVGFAVGAFYAPLTATATRWFVAKRGLAVALVSAGIGFGVLTISPLARWLSS
                     AADWRVAMIVLGDLAWLVILPAALFVREPGPGEAPAAAPVSDGSAGDCDFSRAEVARS
                     PQFWALALTHFACCAAHSGPIFHMVTHAIDQGLASMVAATVLSVSGLASIGGRVATGL
                     LADRFSAKRTLIVGLGLQAVMIFLYLFAQSAPAFYGLALVFGIAYGGVMPLYALLVRE
                     SFGEKVMGTAYGGVFLISTLGMGLGSFAGGWLYDVLGSYGWLFVSSTAIGAAAAVLAL
                     TFRTPRPTPALVPVAGGR"
     gene            8014..9096
                     /locus_tag="VLF19_05980"
     CDS             8014..9096
                     /locus_tag="VLF19_05980"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSE92834.1"
                     /translation="MSDMVRLFWPSLAFVVVTLLALVLRTILLSLLRRLSVRASGLTA
                     FAQSVRVPSLLWCLVLGLYAAIEVASESQHLPRRLSEQLSLLLEVAIVVSVTITVAGV
                     VSTAIRRAGERALGGPVTGLAQTAARGLVLIIGGLVLLGALGIEITPILTALGVGGLA
                     VALALQDTLSNLFAGMHLLADRPIRVGDYVKIAADVEGFVVDVGWRSTRVRMLANNVV
                     VVPNKKVAESIITNYDLPESRMSLLVPVGVSYASEPDHVERVLVEETLAAAKEVPGLL
                     AEPAPFVRLIPGFGDSSLNFTLICQVATFVDQYAAQHELRKRILARFRQEGIEIPFPV
                     RTVQLRQPPALPDQAEERDRADHRQG"
     gene            complement(9028..10239)
                     /locus_tag="VLF19_05985"
     CDS             complement(9028..10239)
                     /locus_tag="VLF19_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92835.1"
                     /translation="MSAAPAPGRAAGRYAILTLILTAQTVANVGPLGIPAIASLIRDD
                     LGLTLAQAGSFLSAYYIGPIVMSLPAGTMADHWGIARTLILGQAVIAVGLLAVSTAGS
                     YPVLIVLLMAAGVGYGMLNPTSTKAVMAWFPPAQRATVIGLKQVGLPFGGALGAALLP
                     ALALALGWRLAVVASAILVGVTAVVSMLVYRDPPDLPPPSAGTRGAVRTVLATRDLWL
                     VAIATLVFAGMQTVWMAFLVLYLKDVVGLTLLAASRYLALAQVAGMAGRVVFGVLSDR
                     TFGGRRRAPLVLAGCGSTVCSLAIAFVGPETSPLVLAPLALVFGFAGIGWNGVQHTLM
                     AELAGARAAGTAVGLGLAVSSIGVTAAPPVFGWCVERVGGYRGPWIGLALTMVGAIAL
                     LGLVREGRRLA"
     gene            complement(10236..10967)
                     /gene="kdsB"
                     /locus_tag="VLF19_05990"
     CDS             complement(10236..10967)
                     /gene="kdsB"
                     /locus_tag="VLF19_05990"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HSE92836.1"
                     /translation="MSLDVLGVIPARLRSTRLPRKVLREIAGRPMLCHVYERARRAPS
                     LGDLLVATDDDEVMDACRRFGIPAVMTSADHPSGTDRVWEIAQSRPADVYVNIQGDEP
                     LLTAGHLERLVEPFRTRPDTQVSTLCIRGTEEELGKTHVNKVVRDVEGRALYFSKYPI
                     PFDRDGIGVVRWKHAGFYAYRRAALETFHRLPPSPLERAEGLEQLRFLEHGVPIVVVE
                     TDEPTIGVDTEDDLKLVEAWLARRA"
     gene            complement(10999..11775)
                     /locus_tag="VLF19_05995"
     CDS             complement(10999..11775)
                     /locus_tag="VLF19_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HSE92837.1"
                     /translation="MIANHVRHALRAGKPQFGTLLNFGDPLVAEMMAATGFDWLLVDT
                     EHGPIDLAALATMFATITRYPVAPLVRVHAVAEENFKRVLDAGAWGVLAPNVRTREQA
                     ELAVSACKYPPAGVRSLGAGRFPLSFKTDPPTYFARANEEILVIVQIEHVEAVKNIDA
                     ILSVPGVDALYVGPNDLCASMGLAPSLEPPHREFDDAMRAVLAAARRHGVAPGIHAAT
                     TGTVNRRIAEGWTLVGMLNDQRLLANAARTIRDAVKTERA"
     gene            12020..12793
                     /locus_tag="VLF19_06000"
     CDS             12020..12793
                     /locus_tag="VLF19_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HSE92838.1"
                     /translation="MTLLEGKTGIVFGVANKRSIAWAIARSLAAAGMRLAFTYQGDRL
                     KENVEELAGTLSGSLLYPCDVTKDEEIAAVFAALGREVGALDTLVHSVAYAQKEDLEG
                     AFVATSREGFRVAHDISAYSLVAVTRAALPLLEKSGQASVIAMTYYGSEKVAGNYNVM
                     GVAKASLEASVRYLAADLGPKGIRANAISAGAVNTLAARGVRGFTSMLQQYAQKAPLR
                     RNVELREIGDTALFLASPMSSGVTGEVIYVDCGYHIMAV"
     gene            12798..14465
                     /gene="hutU"
                     /locus_tag="VLF19_06005"
     CDS             12798..14465
                     /gene="hutU"
                     /locus_tag="VLF19_06005"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HSE92839.1"
                     /translation="MEPSGRPAVPIRAPRGAALSCKGWPQEAALRMLMNNLDPDVAER
                     WQDLVVYGGSGKAARDWPAYHRIVAALRRLEDDETLLVQSGKPVGVFRTHADAPRVLI
                     ANSLLVPAWGDWEHFRAYEAQGLTMYGQMTAGSWIYIGTQGILQGTYETFAACARKHF
                     GGTLAGRLVLTAGLGGMGGAQPLAITLNGGVAIVVEADPDRLERRLRLGYVDRATASM
                     DEAVGWAEEARAARRPLSIGLCGNAAETHPELARRQIRFDVVTDQTSAHDMLNGYVPA
                     GLPAAESAERRRRDPNAYLTSAYESLAAHMRAMLEFQRAGAILFDYGNNIRREAEQAG
                     VGDFAYPGFVPAFIRPLFCEGQGPFRWVALSGDPADIHATDRALMAAFADNEPLVRWL
                     RMAHERVPFQGLPARICWLGYGDRRTAGLIFNDLVASGKVKAPIVIGRDHLDAGSVAS
                     PNRETEAMRDGSDAIADWPVLNALLNAAAGATWVSVHHGGGVGIGYSIHAGMVVVADG
                     TAEARRRLDRVLTTDPGTGIMRHADAGYPEAIDAARRHGLDLPGVTT"
     gene            14462..16009
                     /gene="hutH"
                     /locus_tag="VLF19_06010"
     CDS             14462..16009
                     /gene="hutH"
                     /locus_tag="VLF19_06010"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HSE92840.1"
                     /translation="MSGESAGTIVLDGGRLDLEAIEAIARRGARVKLAPAARRAVAQG
                     RARVERLADAGAPVYGITTGVGKLKDTPIPPADRAQLQTNLVLSHAGGVGAPLAEDEV
                     RALMALLAASLARGASGVRPEVVEVLVTALNAGLYPCVPELGSLGSSGDLAPLAHVAA
                     CLIGEGEAWLEGRRAPAREALGRAGLAPLALEAKEGLALLNGTHLMTGVGALAVLDAE
                     RLARLADVAGAMSLEALMGTNAAFDPRIHELRPHPGQQASAANLRALTVGSAIIASHR
                     DCARVQDAYSLRCMPQVHGSAREAAAFARAVLEREAASVTDNPLVLAGEGLVLSGGNF
                     HGEALGLALDVLAIGLAQLAGIGERRVDRLVNPLTNEGLPPFLSPRGGLHSGYMIAQY
                     IAAALVAECKRLAQPASVDSIPASGLQEDYNAMAAGAALKARGAVRLVRQVVAIELLL
                     AAQALDLRAPLTPGRGSAAARDAVRRRVPRLDEDRYLKGDLDAALTLVEDGSVLQAAE
                     QAVGTLA"
     gene            complement(16040..16435)
                     /locus_tag="VLF19_06015"
     CDS             complement(16040..16435)
                     /locus_tag="VLF19_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92841.1"
                     /translation="MEQHADLSAPDTAVRVREIRRRLPGQMLDERLETARLYYGPLHT
                     LDEIRTRVARTLPHRLGFVRGATLESIETYRDQIPDEALLKWDDAVSKNIFSQYWVAT
                     PTYLGRNQADPWIIGQVTGSALCAVIAQW"
     gene            16520..17383
                     /locus_tag="VLF19_06020"
     CDS             16520..17383
                     /locus_tag="VLF19_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSE92842.1"
                     /translation="MPAATLALVLAAAVLHVGWNALAKRGHHQLCFLWSAQAVAVLLF
                     GPVAAWTLLVDGFDAAALPFVVATILLHAVYFYALGRSYGAGAFSLVYPIARGLGVAL
                     VPVLALAMLDERPSPLGAAGVGLVVLGILVLHAASAGWGRAAAQLRAAGPALWWPVFT
                     GVTIAGYSLVDKAGVARLHPVPYLALMGLGVTALLLPAVRGSGALRAEWRANWRSIVV
                     ASTMTLTSYLLVLFAFRLSKVSYVVAARELSIVLSAVVGSLWLREGALGPRLAGAAVV
                     LAGVVCVALAR"
     gene            complement(17419..17841)
                     /locus_tag="VLF19_06025"
     CDS             complement(17419..17841)
                     /locus_tag="VLF19_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSE92843.1"
                     /translation="MRPWTRNAKPASKAGDLSGFVDEGSQIEGKYTFSGTVMLNGKFS
                     GEIVTSDTLIVGEKGVINAKIRAGNVVISGEVTGNVNATERVELRGTARVFGDVEAPV
                     IVVAEGVLFEGHCRMTKSNGEVAPGAHERDLSVVGVKL"
     gene            complement(18026..19381)
                     /gene="pafA"
                     /locus_tag="VLF19_06030"
     CDS             complement(18026..19381)
                     /gene="pafA"
                     /locus_tag="VLF19_06030"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HSE92844.1"
                     /translation="MKRRIFGLENEYGLTCTLNGQRRLSPDNVARYLFEKVIPGARNA
                     NVFLENGARLYLDTGFHPEYATPECDDVTELVIHDKAGERIVEDLLHQAEKRLREDGI
                     SGNILLFKNNTDSAGNSYGCHENYLVSRDVSFQRLAEALIPFFVTRQIFAGAGKVLQT
                     PRGFHYCLSQRAQHICQEISGATTSSRSIINTRDEPHADAERYRRLHVIVGDSNMSEV
                     ATHLKIGTTALVLDMIEDGFFDRDYSLQSPVQAIRDISHDPTLKETVKLKDGRSISAL
                     QLQLEYLEYATRYVASINPDPATKDVLARWTDVLEKLESDPMELDRELDWVVKRRWIE
                     DYMSRNRLSWRDPKISLMDLQYHDIRPDRGIYYKLVARDMVDRVTDDETIEQAKHVPP
                     QTTRARLRGEFIRQANLKGKDYRVDWVYLKLNDPERETILCKDPFQSHDERVARLIRS
                     F"
     gene            complement(19404..20096)
                     /gene="prcA"
                     /locus_tag="VLF19_06035"
     CDS             complement(19404..20096)
                     /gene="prcA"
                     /locus_tag="VLF19_06035"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSE92845.1"
                     /translation="MPLPYYVSPEQMMKDKAEYARKGISRGRSIVALEYREGILLVAE
                     NPSTLLHKISEIYDRIAFAGVGKYNEFENLRIAGIRHADVKGYSYSRGDVTAKALANA
                     YSQALGNIFTQDIKPFEVEVLVAEVGDENGARNEIYHILYDGTIEDERNYAAMGGQAD
                     DIRRFLKDNYREGMDLADAVQLGVRALNVTQNKSLSERDLEVAVLDRTKTRRKFRRIP
                     SEALAQLLGTTR"
     gene            complement(20099..20914)
                     /gene="prcB"
                     /locus_tag="VLF19_06040"
     CDS             complement(20099..20914)
                     /gene="prcB"
                     /locus_tag="VLF19_06040"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSE92846.1"
                     /translation="MTDQHWQSSFTDYTTSSFIEILQRQSPRLVPRLGETAGTGGPAA
                     VPHGTTVLSLRYRDGVVMAGDRQATAGFQIAHRRIEKIFKCDELSGIGIAGAAGPAME
                     MAKLFQTELEHYEKVEGDNLSLEGKANKLGQMIRMNLQAAMMGLAVVPIFAGFDEKAG
                     VGRLFKYDITGGRYEETDFDAQGSGSMVARDSLKKLWKRDMPRDEALRAGLEAMIDAA
                     DEDVGTGGPDIVRGIFPTMRTITRSGFSEVPEDEVRRLCEAILKDRTRFEQGA"
     gene            complement(21012..22514)
                     /gene="dop"
                     /locus_tag="VLF19_06045"
     CDS             complement(21012..22514)
                     /gene="dop"
                     /locus_tag="VLF19_06045"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HSE92847.1"
                     /translation="MAIPKVMGIETEYGITVKNQPDFNPILSSLLLINSYETYRSSRI
                     RWDYEAESPLRDARGFEYMEEKDVPSKEESRLINLILSNGARFYVDHAHPEYSSPEAT
                     NPRDLVIWDRAGERILNLSRQRAEAVSPPEQRILIYKNNTDYKGNSYGTHENYLMDRK
                     VPFARIVQHMMPFFVTRQVFTGAGKVGAENNADPCDYQISQRADFLETEVGLETMHSR
                     PIINTRDEPHADPEKYRRLHVIVGDANMSEVTNYLKVGTMAIVLSMVEDDFIDCDLSI
                     DGPVAAYRQVSRDLACREAIRLKDGRTITAVDLQREFLALAHRYYRERQQPEPWVSEV
                     LARWESVLDRLAQDPMQLGRELDWVIKHELLENYIAKHDLGWDDSRVQMIDLQYHDVR
                     PGRGLYYKLEEADAVDRMVTDDEISKAIYDPPKDTRAYFRGMCLQRYAEEIVSASWDS
                     VIFDLKEGPLKKIFMLEPLRGTEAHVRGLLTESPTAADLLRNISRPSASV"
     gene            complement(22621..24435)
                     /gene="arc"
                     /locus_tag="VLF19_06050"
     CDS             complement(22621..24435)
                     /gene="arc"
                     /locus_tag="VLF19_06050"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HSE92848.1"
                     /translation="MKEFEKKGSPGREGFSKFLPASMSDSGTRRRLTEYEIQVQDLQA
                     YVRSLEAETVHLRKKLEDMPKEFMVLENKLREANRQLVQAFNQNEKLVNALYEAREQI
                     TSLKEEVDKLCAPPSTYGVYLSVNEDATVNILSQGRKVKVNLHPAIKAEALRPGQELV
                     LNEGLNVVEAAGYEIQGDVVILKEQLDEERAVVTLRADEEKVGIIADPLRTTRLKTGD
                     HILMDAKSGYLLERLPKSEVEDLALEEVPDIGYDDIGGLVTQIEAIKDAVELPYLYAD
                     YYKEHKLTPPKGVLLYGPPGCGKTMIAKAVANNLAEKISEKRGEKIKGYFLNIKGPEL
                     LNKYVGETERKIREIFTKAKEKAADDVPVVVFFDEMDALFRTRGTGISSDVETTIVPQ
                     LLAEIDGVEGLRNVIVIGASNRQDLIDPAILRPGRLDVKIKIERPDRDAASDIFHKYL
                     TTDLPIAEPEIRRHGSAQSAVDAMISAAVESMYQLTEENRFLEVTYANGDKEVLYFKD
                     FSSGAMIESVVRRAKKMALKRHIAGGGKGLNEDDLVAAVREEFKENEDLPNTTNPDDW
                     AKIAGKKGERIVYVKPLMSDARDKQKSVERVVTTGQYL"
     gene            24677..26218
                     /locus_tag="VLF19_06055"
     CDS             24677..26218
                     /locus_tag="VLF19_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92849.1"
                     /translation="MPTPRYVGALGIALAIFAASLTSLSAQPPQPLRREVRLGAPGLP
                     GAIEPVTALEGAAAVIARQVFDTLMTFRESTTDVEPSLATRWVVSRDGLEWSFTIRER
                     VRFHDGTPLTAATVVASFDRHLKAAARSTPTAWASLLRGAPGVVKEVRAVDVRTVQFT
                     LAQPYAALLTLLAHPAFGVAREVSGADGVSRLVGTGPYRVTEASAGRIVIDAVPGHWA
                     GQPRAERLVFLEVGDDDHAEAEFAAGALDVWFPVTAPRRSQWALSTPGPRVGYLAFQT
                     EKEPFSRKKVRRAMAAAIDPAVLGVALGSGAVPLQSFLPAGVWARREGSPVLGGTRKQ
                     VAALLAEGQWPKGFTPTLVAPTESGSVDLKAVAEALQRTLQESDVPVTLRVEAEPAAA
                     EARRAGDHDMAIAEASVAGGDPHLFLYPLSTSEGASKGAQTLNFSFYRNPRLDDMLIR
                     ASQLAYRPERQRLYQRAQAILAEEMPWVPLYVKLLWAVARPEVRGLRLHPSGFHRLTS
                     LGLEG"
BASE COUNT         3766 a         9009 c         9219 g         4357 t
ORIGIN      
        1 ggcctcctga tgctcctcat cttcctgctg gggtggccgc tcgagtggcc ggcgatcatc
       61 ttcatcttcc tgccgatctt catccccgtg gtggtggcgc tcaagttcga cctcatctgg
      121 ttcagcgccc tggtggcgct caacctccag acggcgttcc tctcgccgcc ggtcgccatg
      181 gccgcgtact acctcaaggc cgtcgtgccc tcgtgggagc tcaacgagat ctatcgaggg
      241 atggtcgatt tcatgctgct ccaggtggtc ggcctgatgc tcctcttctt cttcccccag
      301 atcgcgctgt ggcttccgag cctcctctac aagtgagggg agaccgccga accctccgcg
      361 ccggtcgtcc cgcatgagtc cgggtcccga ggagtcgacg ccaccggcca cgcccggtgc
      421 gctggaccgc gtcatcacgg ccatcgacgc cgtgagcacg gtcgcgggct gggtggccgg
      481 ctggctcatc gtgccgatga ccctggccgt gtcgtacgag gtggcggccc gctacgcctt
      541 caacgcgccg accgcctggg cctacgacac cacctacatg ctcttcggcg cccagttcat
      601 gctggcagcg gcctacacgc tgctcaaggg tgggcacatc cgcaccgacg tgttctacga
      661 gcgatggtcg ccacagaccc gggcgaccat cgacgtggtc tcctacctgt tcttcttctt
      721 cccgggcatg ttgttcgtcc tctatgcggg cggggtggag gcctggcact cctggcagat
      781 cggggaacgt tcggactgga ccccctggcg cccgatcctc taccccctga aggcggtgat
      841 cccgctgacg gcggccctcc tggtgctcca gggtttctcc gagctggtca agtgcctgcg
      901 tgtgatccgg gggcgccccc ggtgacccac gagttgctcg ggttcgtcct gctcggcgtc
      961 ttcctcttcg ccatcttcat cgggttcccg gtctccttca ccctgatctt cctgtccctc
     1021 gccttcggct acttcgcgct cggcgacatg gtcttctacc tgaccgtgct gcagacgatc
     1081 gggctcatga aggaggaggt cctggccgcg gtgccgctct tcatcttcat gggctatctc
     1141 ctggaggaag gcgggctcat ggagcggctg ttccatgcct tccgtttgct gatggggggg
     1201 atacccggct ccctctacgt cgccgtgctc ctcaccgcca ccatcttcgg catcgcctcc
     1261 ggtaccgtcg gcgcgacggt cgcggtggtc ggcatcatgt cggtgccgac catgatcaag
     1321 gcgggctacg acccccggct ctcggccgga gccatcaccg ccggcggcag tctcggcatc
     1381 ctgatcccgc cgagcgtgat gctggtcgtg atgggaccgg tgatgggtgt ctcggtggcc
     1441 gacctctacg cggcctcgtt cggcccgggc ttcctgctgg cggggctctt catcgtctgg
     1501 accgtcatcc gctgcctcgt caatccccga ctggggccgc cgttgccgct cgaccagcgc
     1561 tcgggctcgc tccgcaagct cgcgatcgag ctgacgctcg ggatggcccc gcacgtgttc
     1621 ctcaccgtgg tcacgctggg gcccatcctg gccgggatgg cgacgccgac cgaggcggcc
     1681 gcggtggggg tggtgggcac ggcggccatg gccgccctct acggacagct ccggtgggcg
     1741 acgatcaagg aggcggcgat ccagacgggg cagcagacca gcatggtgct gttcctggcc
     1801 gtagcctcca acatcttcgg cgcggtcttc acgcgcctcg ggtcggccac gctgatgacc
     1861 gacaccctca tcgggctgcc gctgccgccc atgggcacgc tgctggtggt catgttcctg
     1921 atcttcttgc tcggctggcc gttcgagtgg ccggcgatcg tgctggtctt cgtgccgctc
     1981 ctccagcccg cgatgatcgc gctgaagttc gaccagctct ggggcgaccc tcatcgccgt
     2041 gaacctccag accgcgttcc tgtcgccgcc ggtggcgatg gccgcctact acctgaaggg
     2101 cgtggcgccc gactggaagc tcaccgacat ctacaagggg atgggggagt tcatggtcct
     2161 ccaggtcatc gggctcctgg tcgtcgtgct ctatccgccg atcgcgctgt ggttcccgga
     2221 gtggctcttc gggaagtagc ccctagagcg ccttctcgtt cgacgggtcg aagaagtgga
     2281 gccggtcggg cagtgctgcc agacgcagcg tctcgtggac cttgacgcgc accgtcggct
     2341 ccacgcgcgc taccagcatg ctcggaccca ccttgacgtc gagcaggatc tccgagccga
     2401 gcggctcgac cacctcgacc accgcgtcga acgcctgatc ggcgaggtcg ccgccgccgc
     2461 cgacgcgaag gtcttccggg cggatgccga gcgtcaggcg ctggcccttg tgcggtgaga
     2521 gacgtgggac cagcgccggc ggcaccttga tccgcaggcc cgggcactcg acccacagcg
     2581 atccgccggc gtcgaccggg gtcacctcca cgaagttcat cgacggcgag ccgatgaagc
     2641 cggccacgaa ccggttcgcg gggtgggagt agagctccag gggctcgccc acctgctgca
     2701 cccagccgtc cttcatcacc acgacgcgat cgcccagcgt catggcctcg acctggtcgt
     2761 gggtgacgta gatggccgtg gtctccagcc ggtcgtgcag gcgcttgagc tccacgcgca
     2821 tctgcacgcg cagcttggcg tcgaggttcg agagcggctc gtcgaagagg aacacctgcg
     2881 ggtggcgcac gatggcgcgc cccacggcca cgcgctggcg ctggccgccc gagagctggc
     2941 gcggcttgcg cttgaggagc tcctggatgc cgaggatctc ggccgcgtcc tgcacgcgct
     3001 tctggatctc ggtcttcggg aacttccgca tcttcagacc gaaggccatg ttgtcgtaga
     3061 ccgtcatgtg cgggtagagg gcgtagttct ggaacaccat cgcgatgtcc cggtccttcg
     3121 gcggcaggtc gttgacgacg cggtcgccga tgctgatctc gcccgcggtg atctcctcga
     3181 gtccggccac catgcggagg gtcgtggact tgccgcagcc ggacggcccg acgagcacga
     3241 tgaactccgt gtcgcggatg tgcaggttca cgtccttgac ggcgtgcact tcgtcgtact
     3301 tcttgttgag gtctttcatc aggacctggg ccatggttct tgtctccccc ctcaggagat
     3361 tcctgatcag cccttcacgg agccggtcag gccggtgacg tagtactcga cgaagaatga
     3421 atagacgagc gccaccggga cggacccgag caatgcgccg gccatgagct gtccccagaa
     3481 atagatgtcg ccccggatca gctcggacac gacccctacc ggcacggtct tgcgctccgg
     3541 cgacgatagg aacacgagcg cgtagatgaa ctcgttccag gacagcgtga acgcgaagat
     3601 gccggccgac aggatcccgg ggacggcgat cgggaagatg atccgcacca tggcaccgaa
     3661 gcgggtggcg ccgtcgatgc gcgcacactc ctcgagctcc ctggggatcg tcttgaagta
     3721 ccccatgagc agccacgtgc agaacggaat caggaacgtc gggtaggtca ggatcagcgc
     3781 ccagggggtg ttgccgagat ggaagctgcg gatgatgtcg gccaggggaa tgaacagcag
     3841 cgtcggcggg acgaggtagg tcacgaagat cgacgtcccg aggacgccgg ccatcgggaa
     3901 gcgcagccgc gcgagcgcgt agccaccgag cagcccgcag aagagcgaga tcccggtgga
     3961 cacgatggcg atgaagaacg tgttccagag ccagcggggg aacgtcgtcc gcgcgagcag
     4021 atcctgcacg tgcgacagcg tcgggtgcac cgtccagaac ggcgcgttgt tgacggcgcg
     4081 ccacgaccgg tacagctcga cgtcgggccg gatggccgtg accagcatcc agtagaacgg
     4141 gaagagcgtc ccgacgacga acacggtgag ggggacgtag aagaacaccc agcgcttcca
     4201 gcggcgctcg atcaccattc acgccccctc gagccggcgc aggtagcgca gctggagcca
     4261 caccacgacg aatagcacgg gcagcatgaa cagcgacacc gccgcgcctt cgccgagcaa
     4321 gcccgtggcg acccccacct ggtaggcgta cgtggccagc aggtgggtcg agttggcggg
     4381 gccgccgccc gtgagcacgt agatgagctg gaagtcggag aaggtctgga tgaccgagaa
     4441 gacgacgacg acgatggtca cgggcttcag caggggccag gtgacgtgcc agaagcgctt
     4501 ccacccgttg gccccgtcga gcgcggcggc ctcgtggagg tcggggttga tggtctgaag
     4561 cccggccagc agggtgatcg cgaagaaagg catgccgcgc cacgtgttca cgacgatggc
     4621 gcaccagaga gcccagacgc cgtccgagag gaagggcagc ttcgtcacga tgaagccggt
     4681 cttgtacaac gtccagttga tcacgctgaa ggtggggtcg aacatccacc gccacgcgaa
     4741 cgtcgagagc acggtgggga tgatgaacgg cagtagcatc gaggcgcgca cgagccgctt
     4801 gccgcggaag tgccggttga gaagcagggc cagccacatc ccgagcacga gcttgaacag
     4861 cgtcgcccag aacgtgtaga agaacgtgtt gcggaaggcc gtctggaaga tggagtcgtt
     4921 ccaggccttc gcgaagttgg tcagcccgac gaagaccccg ggattgccga cgctggtgct
     4981 cgacagcgac agccacaccc ccatgacgaa cggataggcg atgaacagac cgagcaggac
     5041 gaccgtgggg gcgagcagca gcgtggcgag ggcgcgctcg tcctccagca cccgcgccag
     5101 cggccggcgc tgcgcggggg ttcggggaag cgccacctcg acgcccgccg cgcgccccga
     5161 gcctcccgcg atcggcctgt tcacgccacc tcagggcggg ctccgcgcga gcccgctacg
     5221 cctcgtagat cttcttcagc tcggcctcgg cccatttcac ggcgtcttcg gccttcgtgc
     5281 cctgaacggc cttggcgtac atgtcggtga tgatgtactt ggagtagacc tccgtcgact
     5341 tcgccgtcga gggcccggcg tacccgaaga tgcgcgtctc gcgcgctgcc gtccggaaga
     5401 tctgcagggg cttgtcgatc ttgccccaca tcgggtggtt ctcccacgac ttggtggccg
     5461 cgacgctgta cccttcggcg atctggaacc acttctcgta gttctccttc ttgtggagcc
     5521 acgtcaggaa gtccttcgcc agcttctggt tcttcgagta cttcatgacc ccgtgggcgt
     5581 tggcgaagta cagcgggtac acgcccgccc cgcccttgag cgggaagatc gcgtgatcga
     5641 tgtcctggaa cagcggctcg cccttgtcgt ccttcagctt gtccttctgc cgcttggcga
     5701 cgatgtagat cgaggcgccg ttgagggtgg cgcagacctc ccccgcctgg aaggcgcggt
     5761 tgttgttcga gtcgtcccag gccagcccgc cctcgtcgca gccgtccttc cagagcgctt
     5821 gcatgtactt caccgagtcg agggtaccct tcgagttgat ggccaccttc ttgccgctct
     5881 tgtccgtctc ggcgcccccg aaggaccaca tgagcgggta cgtgaaggtc ggggcgtccc
     5941 cgaaggtgtg tccgagcgtc tgcccgtagg gtttgccctt cttcttcagc gccgtcgccg
     6001 ccttgcggag gtcgtcccag gtctcgggaa acttgtcgaa gccgacctct ttgaaccacg
     6061 accggcggta ggcgatggcg ttgccgacga tcgagtgggg catgccgagc cacttgccgc
     6121 ccacccgggc ggcggcgttg aagacctcgt agaacccgcc ttgctccttc gccaccgcgg
     6181 tggcgacgtc ggagacgtcg acgagggcgt tctggtaaag gtgcgcccaa ttgtagtaga
     6241 gcatgaagat atccgcgccg gctccggact gaacggcggc cgtgatgcgt ggctggaggt
     6301 cgttgtaccc gatggtctcc agcaccacct ccgcgccgag ggccttcgag gcagccgcca
     6361 cggcctcgtt cttcaggtgg acgtcgcact cggggatgaa gtcgttccag cgcacccagt
     6421 gcagcttcgt cccctgcgcc caggccggcg cgcgcccggc ggcgaggatg ccctcgatcc
     6481 ctgtcgaggc tacgactgcg gccgcgcccg acagcttcag gaaatacctc cgattcgagt
     6541 cgtgctcgct catccgtgag tcctccttgg gtcggggcgc agacccgggc cgtgggcgcc
     6601 gtaagtgcgg tccaaaccga gaaaagtgcc gatttgatac caccccgggg gcgggggtgt
     6661 caaccgcgag ccgcccgccg ggtgccgtgc ccggggtgca ggcggggggg cagggttcgt
     6721 agcgaccgtg agagacggcc gacggtgagc ggctcgggcg gctcagcggc cgccggcgac
     6781 ggggacgagc gcgggtgtcg ggcgcggcgt acggaacgtg agggcgagca ccgcggcggc
     6841 ggcgccgatg gccgtggagc tcacgaagag ccagccgtag ctcccgagga cgtcgtagag
     6901 ccagccaccg gcgaacgaac cgagccccat gccgagcgtg gagatcagga acacgccgcc
     6961 gtacgccgtg cccatgacct tctcgccgaa ggactcgcgc acgagcagcg cgtagagcgg
     7021 catcacgccg ccataggcga tgccgaacac cagcgcgagt ccgtagaacg ccggggcgct
     7081 ctgcgcgaac aggtagagga agatcatgac ggcctgcagg ccgagcccga cgatgagcgt
     7141 gcgcttggcg ctgaagcggt ccgcgagcag gccggtggcc acccgcccgc cgatcgaggc
     7201 gaggcccgac accgagagga ccgtggccgc caccatcgag gcgagcccct ggtcgatcgc
     7261 gtgcgtgacc atgtggaaga tcgggcccga gtgcgccgcg cagcaggcga agtgggtcag
     7321 cgcgagggcc cagaactgcg gcgaccgcgc cacctcggcc cgcgagaagt cgcagtcccc
     7381 ggcactgccg tctgacaccg gtgccgcggc cggcgcctcg ccgggcccgg gctcgcgcac
     7441 gaagagggcc gcgggcagga tcacgagcca tgcgaggtcg ccgagcacga tcatggcgac
     7501 ccgccagtcc gcggcgctcg acagccagcg cgccagcggc gagatcgtca ggacgccgaa
     7561 gccgatcccg gccgagacca gcgcgacggc caggccgcgc ttggcgacga accaccgggt
     7621 cgccgtcgcc gtgagcgggg cgtagaaggc gccgacggcg aagcccaccg tgacgccgaa
     7681 ggtgacgtag agctgccaca gcgccgtcac ctggctggag agcaccaggc ccagcccgag
     7741 cagagcgcca ccgcccagca cgaccacgcg gccgccgtag cggtcggaga gcgcgcccca
     7801 gacgagcgag ccgagcccca tcgcgatcca gttgatgagc gcgatcgtgg agatgccggt
     7861 ccggctccag cccatcgatt cctcgatggg cttgaggaag atgcccatcg agaagagcgc
     7921 gcccatgccg acgcacgtga tgagcgcgcc gacgccaacc acgacccagc cgtaggaaat
     7981 tgatcgctca cgcatgacgg cacctacgat agcatgagcg acatggtccg tctcttctgg
     8041 ccgagcctgg cgttcgtcgt ggtgacgctg ctggcgctgg tgctgcggac catcctgttg
     8101 tcgctgctgc ggcggctgtc cgtacgcgcg agcgggctca cggccttcgc ccagagcgtg
     8161 cgggtgccct cgctgctctg gtgcctggtg ctcggcctct acgcggccat cgaggtcgcg
     8221 agcgagtcgc agcacctccc gcgacggctc agcgagcagc tctcgctcct gctcgaggtg
     8281 gccatcgtcg tgtccgtcac gatcacggtg gccggcgtgg tgagcacggc catccggcgc
     8341 gccggggagc gggcgctcgg cggccccgtc accgggctcg cccagacggc cgcgcgcggc
     8401 ctcgtgctca tcatcggcgg gctggtgctg ctcggggcgc tgggaatcga gatcaccccg
     8461 atcctcaccg ccctcggcgt cggcggcctg gccgtcgccc tcgcgctcca ggacacgctc
     8521 tccaacctgt tcgcgggcat gcacctgctg gcggatcgcc cgatccgcgt gggggactac
     8581 gtcaagatcg ccgccgacgt cgagggcttc gtggtggacg tgggctggcg gtccacgcgc
     8641 gtccggatgc tggcgaacaa cgtcgtcgtg gtgccgaaca agaaggtcgc ggagtccatc
     8701 atcaccaact acgacctgcc cgagtcgcgg atgtcgctgt tggtgccggt tggggtcagc
     8761 tatgccagcg agccggacca cgtggagcgc gtgctcgtcg aggagacgct ggccgcggcg
     8821 aaggaggtgc ccggcctgct cgccgagccg gcgccgttcg tgcggctcat cccgggcttc
     8881 ggcgactcct cgctgaactt cacgctgatc tgccaggtgg cgacgttcgt ggatcagtac
     8941 gcggcccagc acgagctgcg caagcgcatc ctggcccgat tccggcagga gggcatcgag
     9001 atcccgttcc ccgtgcggac ggtgcagcta cgccagccgc cggccctccc ggaccaggcc
     9061 gaggagcgcg atcgcgccga ccatcgtcag ggctagaccg atccacggcc cgcggtagcc
     9121 gcccacccgc tcgacgcacc agccgaagac cggaggcgcc gccgtcacgc cgatcgacga
     9181 gacggccagc cccaggccca ccgcggtgcc ggccgcgcgg gcgccggcca gctcggccat
     9241 gagcgtgtgc tgcacgccgt tccacccgat cccggcgaag ccgaagacga gcgccagcgg
     9301 cgccagcacg agcggcgagg tctccgggcc gacgaaggcg atggccagcg agcacaccgt
     9361 cgagccgcag ccggccagca cgagcggggc gcggcggcgg cccccgaagg tgcggtcgga
     9421 gagcacgccg aacacgaccc ggccggccat gcccgcgacc tgcgcgagcg cgagatagcg
     9481 gctggccgcg agcagcgtga gcccgacgac gtccttgagg tagaggacga ggaatgccat
     9541 ccacaccgtc tgcatgccgg cgaagacgag cgtggcgatc gcgacgagcc agagatcgcg
     9601 cgtggcgagg acggtgcgca cggctccacg ggtgccggcc gacgggggcg gcaggtcggg
     9661 cgggtcgcgg tagacgagca tcgagacgac ggccgtcacc ccgacgagga tggccgaggc
     9721 gacgacggcc agccgccagc cgagcgccag cgcgagcgcc ggcagcagcg cggcgcccag
     9781 cgcgccgccg aacggcaggc ccacctgctt gaggccgatg accgtggcgc gctgcgccgg
     9841 cgggaaccag gccatgacgg ccttcgtcga ggtcgggttc agcatgccgt accccacgcc
     9901 ggcggccatc agcagcacga tgagcaccgg ataggatccc gccgtgctca ccgccagcag
     9961 gccgaccgcg atgaccgcct gcccgaggat cagcgtgcgc gcgatgcccc agtggtcggc
    10021 catcgtcccc gccggcagcg acatgacgat cgggccgatg tagtacgcgc tgaggaacga
    10081 cccggcctgc gcgagcgtga gccccaggtc gtcgcggatc agcgaggcga tggccgggat
    10141 gccgaggggg ccgacgttgg cgacggtctg cgctgtcagg atcagggtga ggatcgcgta
    10201 gcgccccgcc gcgcgaccgg gtgcgggcgc ggcgctcacg cccggcgcgc cagccaggcc
    10261 tcgaccagct tgaggtcgtc ctcggtgtcc acgccgatcg tcggctcgtc ggtctccacc
    10321 acgacgatcg ggacgccgtg ctccaggaag cgcagctgct cgagcccctc cgcgcgctcg
    10381 agcggtgagg gtggcaggcg gtggaaggtt tccagcgcgg cccgccggta ggcgtagaag
    10441 cccgcgtgct tccagcgcac gacgccgatt ccgtcgcggt cgaacgggat cgggtacttc
    10501 gagaagtaga gcgcccggcc ctcgacgtcg cgcacgacct tgttgacgtg tgtcttgccg
    10561 agctcctcct cggtcccccg gatgcagagc gtgctcacct gtgtgtccgg gcgggtccgg
    10621 aagggctcca cgagccgctc gaggtggccg gcggtgagga gcggctcgtc gccctggatg
    10681 ttgacgtaca cgtcggcggg ccggctctgg gcgatctccc acacgcggtc ggtgcccgaa
    10741 gggtggtcgg ccgaggtcat caccgccgga atgccgaagc gccggcacgc gtccatgacc
    10801 tcgtcgtcgt ccgtggccac gagcaggtcg ccgagcgagg gcgcacggcg ggcgcgctcg
    10861 tagacgtggc agagcatggg ccgccccgcg atctcgcgca gcaccttgcg cggcagccgg
    10921 gtggagcgca gccgggcggg aatcacgccg aggacgtcga ggctcacggc gggacgccgt
    10981 gggtacgacc ggcccgcgtc acgcgcgctc ggtcttgacg gcgtcgcgga tggtgcgcgc
    11041 ggcgttggcg agcagccgct ggtcgttcag catcccgacg agcgtccacc cctccgcgat
    11101 gcggcggttg acggtgcccg tggtggccgc gtggatcccc ggcgccacgc cgtgacggcg
    11161 cgccgccgcc agcaccgctc gcatggcgtc gtcgaactct cggtggggcg gctcgagcga
    11221 cggggcgagg cccatggagg cgcagaggtc gttcgggccg acgtagagcg cgtccacgcc
    11281 cggcacggac aggatcgcgt cgatgttttt cacggcctcg acgtgctcga tctggacgat
    11341 gacgaggatc tcctcgttcg cgcgcgcgaa gtacgttggc gggtcggtct tgaacgagag
    11401 cgggaagcgc ccggcgccga ggctccgcac gccggctggc ggatacttgc aggcggacac
    11461 ggcgagctcg gcctgctcgc gcgtgcgcac gttcggcgcg agcacgcccc aggcgccggc
    11521 gtcgagcacc cgcttgaagt tctcctcggc gacggcgtgc acgcggacga gcggcgccac
    11581 cgggtagcgc gtgatcgtgg cgaacatggt cgcgagggcc gcgagatcga tggggccgtg
    11641 ctcggtatcg acgagcagcc agtcgaatcc ggtggcggcc atcatctcgg cgacgagcgg
    11701 gtcgccgaag ttcagcagcg tgccgaactg cggcttgccg gcccgcagcg cgtgcctcac
    11761 gtggttcgcg atcacgacgt ctcctgttct tgccgtccgg gcgtgactcc gaccatttca
    11821 ccgctcgcct cgccacccga gcgtggctca gctcgcactg cggatcattt caccgcttgc
    11881 ctcgccaccc cggcgcggat cgggtcgcag gcagcccgaa gtcgggcagt gaaccctcgt
    11941 tatctctccg cgatgatgac gacgggcgga gtatacaccg cgcgccaccg gccgtatcat
    12001 ggcgcgggag gacgcgcgga tgacactcct cgagggcaag accggcatcg tcttcggtgt
    12061 cgccaacaag cgctcgatcg cgtgggccat cgcccggtcg ctcgccgcgg ccggcatgcg
    12121 tctggccttc acctaccagg gcgaccgcct gaaggagaac gtcgaggagc tcgccggcac
    12181 gctgtctggg tcgctgctct atccctgcga cgtgacgaag gacgaggaga tcgcggcggt
    12241 gttcgcggcg ctcgggcgcg aggtcggcgc gctcgacacg ctcgtgcact cggtggccta
    12301 tgcccagaag gaggacctgg agggcgcgtt cgtcgcgact tcgcgcgagg ggttccgcgt
    12361 ggcccacgac atctcggcct actcgctggt ggccgtcacg cgggcggcgc tcccgctact
    12421 cgagaagtcc ggccaggcct cggtgatcgc catgacctac tacggctccg agaaggtcgc
    12481 cggcaactac aacgtgatgg gggtggcgaa ggcctcgctg gaggcctcgg tgcgctacct
    12541 ggcggccgac ctcggcccca agggtatccg cgccaacgcc atctccgccg gcgccgtcaa
    12601 cacgctggcc gcgcgcggcg tccggggctt cacctcgatg ctgcagcagt acgcgcagaa
    12661 ggcgccgctc cgccgcaacg tggagctgcg cgagatcggc gacaccgcgc tgttcctcgc
    12721 ctcgccgatg tcctcgggcg tcacgggcga ggtcatctac gtcgattgcg gctaccacat
    12781 catggcggtg tagccgcgtg gagccgtccg ggcggcccgc cgtccccatc cgcgcgccgc
    12841 gcggcgcggc gctgtcttgc aagggctggc cgcaggaggc cgcgctccgg atgctgatga
    12901 acaatctcga tccggacgtc gccgagcgct ggcaggacct cgtggtctac gggggctcgg
    12961 gcaaggctgc ccgcgactgg cccgcctacc accgcatcgt cgcggcgctc cggcggctcg
    13021 aggacgacga gacgctgctg gtgcagtcgg gcaagcccgt cggtgtcttt cgcacccatg
    13081 ccgacgcgcc ccgtgtgctc atcgcgaact cgctgctggt cccggcgtgg ggcgactggg
    13141 agcacttcag agcctacgag gcccagggcc tgacgatgta cggccagatg acggccggct
    13201 cgtggatcta catcggcacg cagggcatat tgcagggcac gtacgagacg ttcgccgcgt
    13261 gcgcgcgcaa gcacttcggg gggacgctgg cgggccgcct cgtgctcacc gccggcctgg
    13321 ggggcatggg aggcgcgcag ccactggcca tcacgctcaa cggtggcgtg gccatcgtcg
    13381 tcgaggccga tcccgaccgg ctcgagcggc gcctgcgcct gggctacgtc gatcgcgcga
    13441 cggcctcgat ggacgaggcc gtcggctggg ccgaggaggc gcgcgcggcg cggcggccgc
    13501 tgtcgatcgg gctgtgcggc aacgccgccg aaacgcatcc cgagctggct cgccgccaga
    13561 tccgcttcga cgtcgtgacc gatcagacgt cggcccacga catgctgaac gggtacgtgc
    13621 cggccggcct gccagcggcc gagtccgcgg agcggcgccg ccgcgacccg aacgcgtacc
    13681 tgacgtccgc gtacgagtcg ctcgccgcgc acatgcgcgc gatgctcgag ttccagcgcg
    13741 cgggcgcgat cctcttcgac tacggcaaca acatccgccg cgaggccgag caggcgggcg
    13801 tcggcgactt cgcctatccc ggtttcgtcc ccgccttcat ccgtccgctc ttctgcgagg
    13861 ggcaggggcc cttccgctgg gtcgcgctct ccggggatcc cgcggacatc cacgccaccg
    13921 accgggcact gatggcggcg ttcgcggaca acgagcccct cgtccgctgg ctccgcatgg
    13981 cgcacgagcg cgtgccgttc caggggctgc cggcccgcat ctgctggctc ggctacggcg
    14041 accggcggac ggcggggctg atcttcaacg acctcgtggc ctcggggaag gtgaaggcgc
    14101 cgatcgtgat cggccgtgac cacctcgacg cgggttcggt cgcctcgccg aaccgcgaga
    14161 ccgaggccat gcgcgacggg tccgacgcga tcgcggactg gccggtgctg aacgcgctcc
    14221 tcaacgctgc cgcgggcgcg acgtgggtca gcgtccacca cggtggcggg gtcggcatcg
    14281 gctactcgat ccacgcgggc atggtggtcg tcgccgacgg cacggcggag gcgcggcgcc
    14341 gtctcgatcg cgtgctcacg accgaccccg gcaccggcat catgcgccac gccgacgccg
    14401 gctacccgga ggccatcgac gccgcgcgcc ggcacgggct cgacctcccg ggcgtgacga
    14461 cgtgagcgga gagagcgcgg gcacgatcgt cctcgacggg ggccggctgg acctggaggc
    14521 gatcgaggcc atcgcgcgcc gtggcgcgcg cgtgaaactg gcgccggccg cccgccgcgc
    14581 cgtcgcccag ggccgcgccc gcgtcgagcg tctcgcggac gcgggagccc ccgtgtacgg
    14641 gatcaccacg ggcgtgggca agctcaagga tacgccgatc ccgccggctg atcgcgcgca
    14701 gctgcagacg aatctggtgc tgagccacgc cggcggggtg ggcgcgccgc tggccgagga
    14761 cgaggtgcga gcgctcatgg cactgctcgc cgcctcgctc gctcgcggcg cctccggtgt
    14821 gcggcccgag gtcgtcgagg tcctggtgac cgcgctcaac gccggcctgt atccctgcgt
    14881 gccggagctg ggctcgctcg gctcgtcggg cgacctcgcg cccctcgcgc acgtggccgc
    14941 ctgtctcatc ggcgagggcg aggcctggct cgaggggcga cgcgcgccgg cgcgcgaggc
    15001 tctggggcgg gcggggctgg ccccgctcgc gctcgaggcg aaagagggcc tggcgctcct
    15061 caacggcacc cacctgatga cgggcgtggg cgcgctggcc gtgctcgacg cggaacggct
    15121 ggcgcgcctg gccgacgtcg cgggcgcgat gtcgctcgag gcgctcatgg gcacgaacgc
    15181 cgcgttcgac ccgcggatcc acgaactgcg cccgcatccc ggccagcagg cgtccgcggc
    15241 gaatctccgt gcgctgacgg tcggctcggc catcatcgcc tcgcaccggg actgcgcgcg
    15301 cgtgcaggac gcctacagcc tgcgctgcat gccgcaggtg cacggctcgg cgcgcgaggc
    15361 ggcggccttc gcccgcgccg tcctggagcg cgaggcggcc agcgtcaccg acaaccccct
    15421 ggtcctcgcc ggcgaggggc tggtgctgag cggcgggaac ttccacggcg aggcgttggg
    15481 cctcgccctc gacgtgctcg ccatcgggct ggcgcagctg gccgggatcg gcgagcggcg
    15541 cgtcgatcgc ctcgtgaacc cgctgaccaa cgagggcctg ccgccgttcc tctcgccccg
    15601 tggcgggctg cactccggct acatgatcgc gcagtacatc gccgcggcgc tggtcgcgga
    15661 gtgcaagcgc ctggcgcagc ccgccagcgt cgactcgatc cccgcctcag gcctgcagga
    15721 ggactacaac gcgatggcgg ccggcgccgc cctcaaggcg cgcggggccg tgcgcctcgt
    15781 ccgccaggtg gtggccatcg agctgctgct cgccgcccag gcgctcgacc tgcgggcgcc
    15841 gctgacaccg ggacgcggca gcgccgccgc gcgggacgcc gtgcgccgcc gtgtgccgcg
    15901 cctggatgag gaccgttacc tcaagggcga cctcgacgcg gccctgaccc tggtcgagga
    15961 cggcagcgtc ctgcaggccg cggagcaggc cgtcggcacg ctggcctgag tcacgcccgg
    16021 gagatcgcgc gcgccctggt caccactggg cgatgacggc gcagagggcg gagcccgtga
    16081 cctgcccgat gatccagggg tccgcctggt tccggccgag gtaggtcggg gtggccaccc
    16141 agtactgcga gaagatgttc ttgctcaccg cgtcgtccca cttcagcagc gcctcgtcgg
    16201 ggatctggtc gcggtaggtc tcgatcgact cgagcgtcgc accgcgcacg aacccgagcc
    16261 gatggggaag ggtccgggcg accctggtac ggatctcgtc cagcgtgtgg agcggaccgt
    16321 agtagagccg cgcagtctcg agacgctcgt cgagcatctg gccgggcagc cggcggcgga
    16381 tctcgcggac gcggacggcg gtgtcggggg cggagaggtc ggcatgctgt tccatgaagg
    16441 cctccttggc ggcgtggcga tggatcgcct ccgtgcaatc ggggtgccgg tacgcccggt
    16501 tccagtatag ttcgggtcga tgccggctgc caccctcgcg ctcgtgctcg ccgctgccgt
    16561 cctgcacgtc ggctggaacg cgctcgccaa gcgcggccac caccagctct gcttcctgtg
    16621 gtcggcgcag gccgttgccg tgctgctctt cgggccggtg gctgcgtgga cgctgctggt
    16681 ggacgggttc gacgcggcgg cgctgccctt cgtcgtcgcc acgatcctcc tgcacgcggt
    16741 ctacttctac gcgctgggcc gctcgtacgg cgccggcgcg ttttccctgg tctatccgat
    16801 cgcgcggggt ctgggcgttg ccctggtgcc ggtcctggcg ctcgcgatgc tcgacgagcg
    16861 cccgtcgccg ctcggcgcgg ccggtgtggg gctcgtcgtc ctcggcatcc tcgtcctcca
    16921 cgccgcctcg gcgggatggg gtcgggccgc cgcgcaactc cgcgcggccg gaccggcgct
    16981 gtggtggccg gtcttcacgg gcgtcaccat cgccggctac tcgctggtgg acaaggcggg
    17041 ggtggcgcgg ctgcaccccg tgccgtacct ggccctcatg gggctcggcg tgacggcgct
    17101 gctgctgccc gccgtccgtg gcagcggcgc gctgcgcgcg gagtggcggg cgaactggcg
    17161 gtcgatcgtg gtggcgtcga cgatgacgct gacgagctac ctgctggtgc tcttcgcgtt
    17221 ccggctctcg aaggtcagtt acgtcgtcgc cgcccgcgag ctgtcgatcg tgctgtccgc
    17281 ggtcgtcggc agcctctggt tgcgcgaagg ggcgctgggg ccgcgcctgg cgggggcggc
    17341 agtggtgctg gcgggggtgg tctgcgtcgc gctggcgcgc tagagcgctc gaagggggct
    17401 tcgttccttt ccgtccgtct agagcttgac gccgacgacc gagaggtcgc gctcgtgcgc
    17461 gccgggcgct acctcgccgt tggacttggt catgcggcag tgcccctcga agaggacgcc
    17521 ctccgcgacc acgatcaccg gcgcctccac gtcgccgaag acccgcgcgg tgccgcgcag
    17581 ctccacgcgc tcggtggcgt tcacgttgcc ggtgacctcg ccgctgatga ccacgttgcc
    17641 agcgcggatc ttcgcgttga tgacgccctt ctcgccgacg atcagcgtgt cgctcgtgac
    17701 gatctcgccg gagaacttgc cgttcagcat gacggtgccg ctgaacgtgt attttccctc
    17761 gatctgcgag ccctcgtcga cgaagccgct caggtcgccg gccttggagg ccggcttggc
    17821 gttcctggtc cagggacgca tgggatgggg cgctcctcct tcgtcgcttc gcgcgcgtcg
    17881 aaagcgcgtc gaagacttcc gtactccagc gttagcattc ttcgagaacc ggagtcaatt
    17941 tgccccgacg gcacttccgg tggggcccga cacaccggcg ccccactagc gcacgggtgt
    18001 gagcgcgggg ccgggcgatg gcgcgctaga acgagcggat caggcgggcg acccgttcgt
    18061 cgtgcgactg gaacggatcc ttgcagagga tcgtctcgcg ctccgggtcg ttgagcttca
    18121 ggtagaccca gtccacgcgg tagtccttgc ccttcaggtt cgcctggcgg atgaactcgc
    18181 cgcgcagccg cgcccgcgtg gtctgcggcg ggacgtgctt ggcctgctcg atggtctcgt
    18241 cgtcggtgac acgatcgacc atgtcgcgtg ccaccagctt gtagtagatc ccgcggtcgg
    18301 gccggatgtc gtggtactgg aggtccatca gcgagatctt cgggtcgcgc cacgacagcc
    18361 ggttgcgcga catgtagtcc tcgatccagc ggcgcttgac gacccagtcg agctcccggt
    18421 cgagctccat ggggtcggac tcgagcttct cgagcacgtc cgtccaccgc gcgaggacgt
    18481 ccttggtggc cgggtcgggg ttgatcgagg ccacgtagcg ggtggcgtac tcgagatact
    18541 cgagctggag ctggagcgcg ctgatggacc gcccgtcctt gagcttgacc gtctccttca
    18601 gcgtcgggtc gtgcgagatg tcccggatgg cctggaccgg cgactgcagc gagtagtcgc
    18661 ggtcgaagaa cccgtcctcg atcatgtcca gcacgagcgc ggtggtgccg atcttgaggt
    18721 gggtggccac ctccgacatg ttcgagtcgc ccacgatgac gtgcaggcgg cggtagcgct
    18781 cggcgtcggc gtggggctcg tcgcgcgtgt tgatgatcga gcgggacgag gtggtcgccc
    18841 cggagatctc ctggcagatg tgctgggctc gctgactcag gcagtagtgg aagccgcggg
    18901 gtgtctggag gaccttcccg gcccccgcga agatctgccg ggtgacgaag aaggggatga
    18961 gcgcttcggc cagccgctgg aaggacacgt cgcggctgac gagatagttc tcgtggcagc
    19021 cgtacgagtt accggcagag tcggtgttgt tcttgaagag caggatgttc ccggagatgc
    19081 cgtcctcgcg cagccgcttc tcggcctgat gcagcaggtc ctcgacgatg cgctcgccgg
    19141 ccttgtcgtg gatgacgagc tcggtgacgt cgtcgcactc gggggtggcg tactcgggat
    19201 ggaagcccgt gtccaggtag aggcgagcgc cgttctcgag gaacacgttg gcgttgcggg
    19261 cgccggggat gaccttctcg aacaggtaac gggcgacgtt gtccgggctc agccgacgct
    19321 ggccgttcag cgtgcaggtg agcccgtact cgttctcgag cccgaagatc cggcgcttca
    19381 tcttacccag agggtagcac ggcctagcgc gtcgtgccga ggagttgcgc cagcgcctcg
    19441 gacgggatgc ggcggaactt ccggcgcgtc ttggtgcggt ccagcaccgc gacctcgagg
    19501 tcccgctcgc tcagggactt gttctgcgtc acgttcagcg cgcgcacccc gagctgcacc
    19561 gcgtcggcga ggtccatgcc ctcgcggtag ttgtccttca ggaagcggcg gatgtcgtcc
    19621 gcctgaccgc ccatggccgc gtagttgcgc tcgtcctcga tcgtgccgtc gtagaggatg
    19681 tgatagatct cgttgcgggc accgttctcg tcccccacct cggccaccag cacctcgacc
    19741 tcgaacggct tgatgtcctg cgtgaagatg ttgcccagcg cctgcgagta ggcgttggcc
    19801 agggccttcg ccgtgacgtc gccacgactg taggagtagc ccttgacgtc cgcgtggcgg
    19861 atgccggcga tccggaggtt ctcgaactcg ttgtactttc cgaccccggc gaaggcgatg
    19921 cggtcgtaga tctcggagat cttgtgcaac agggtggagg ggttctcggc gacgagcagg
    19981 atcccctcgc ggtactcgag cgccacgatg gaacgcccgc gggagatgcc cttgcgggca
    20041 tactccgcct tgtccttcat catctgctcg gggctgacgt agtagggcaa cggcacgctc
    20101 aggctccctg ctcgaacctg gtacggtcct tcaagatggc ctcgcagagc ctgcgcacct
    20161 cgtcctccgg gacctcgctg aagccggagc gggtgatcgt gcgcatggtc gggaagatgc
    20221 cacggacgat gtccggcccg ccggtgccga cgtcctcgtc ggcggcgtcg atcatcgcct
    20281 cgaggcccgc gcgcagcgcc tcgtcgcgcg gcatgtcgcg cttccacagc ttcttgagcg
    20341 aatcgcgggc gaccatcgag cccgagccct gcgcgtcgaa gtcggtctcc tcgtaccggc
    20401 ccccggtgat gtcgtacttg aagagccggc cgacgcccgc cttctcgtcg aagcccgcga
    20461 agatcggcac cacggccagc cccatcatcg ccgcttgcag gttcatgcgg atcatctgcc
    20521 cgagcttgtt ggccttgccc tcgagcgaca ggttgtcgcc ttccaccttc tcgtaatgct
    20581 cgagctcggt ctggaacagc ttggccatct ccatggcggg gccggcggcc ccggcgatgc
    20641 cgatgccgct gagctcgtcg cacttgaaga tcttctcgat ccgccggtga gcgatctgga
    20701 agccggccgt cgcctgccga tcccccgcca tcaccacccc gtcgcgatac cggagcgaca
    20761 acaccgtcgt cccgtgcggt accgcagcgg gcccgcccgt ccccgcagtc tcgccgagcc
    20821 ggggcaccag gcggggcgac tgccgctgca ggatctcgat gaagctggac gtcgtgtagt
    20881 cggtgaagct cgactgccag tgctggtccg tcatcggccg cctattctac cccgcgctgg
    20941 gtgtcttctc gcgcgacggt cgggcgcttc gagcggcggc gttgccgccg aaatccgagg
    21001 cccccttcga ttcatacact ggcgctgggg cgcgagatat tgcgcagcag gtccgcggcc
    21061 gtgggggact cggtgaggag gccgcgcacg tgcgcctccg ttccccgcag aggctccagc
    21121 atgaagatct tcttcagcgg accctccttg aggtcgaaga tgacggagtc ccaggacgct
    21181 gacacgatct cctcggcgta tcgctgcagg cacatgcccc ggaagtaggc ccgggtgtcc
    21241 ttgggcgggt cgtagatcgc cttcgagatc tcgtcgtcgg tgaccatgcg gtcgacggcg
    21301 tcggcctcct ccagcttgta gtagaggccg cgccccggcc gcacgtcgtg atactgaagg
    21361 tcgatcatct gtacgcgcga gtcgtcccag cccaggtcgt gcttggcgat gtagttctcg
    21421 aggagctcgt gcttgatcac ccagtcgagc tcccggccga gctgcatggg gtcctgggcg
    21481 aggcgatcga gcaccgactc ccagcgggcc agcacctcgg acacccacgg ctccggctgc
    21541 tgccgttccc ggtagtagcg gtgcgccagc gccaggaact cccgctgcag gtccaccgcg
    21601 gtgatggtcc gtccgtcctt cagccggatc gcctcgcggc aggcgaggtc gcgcgacacc
    21661 tgccggtagg cggccaccgg cccgtcgatc gacaggtcac agtcgatgaa gtcgtcctcg
    21721 accatcgaga gcacgatggc catggtgccg accttcaggt agttggtcac ctcgctcatg
    21781 ttggcgtcgc ccacgatcac gtgcaggcgc cggtacttct cggggtcggc gtgcggctcg
    21841 tcgcgggtgt tgatgatggg ccgggagtgc atggtctcca gacccacctc ggtctccagg
    21901 aagtccgcgc gctgcgagat ctggtagtcg cacgggtcgg cgttgttctc ggcgcccacc
    21961 ttgccggcac cggtgaagac ctgacgggtc acgaagaacg gcatcatgtg ctgcacgatg
    22021 cgtgcgaacg gcaccttgcg gtccatcagg tagttctcgt gggtgccgta cgagttgcct
    22081 ttgtagtcgg tgttgttctt gtagatcagg atccgctgtt ccggcggaga gaccgcctcc
    22141 gctctctgtc gggagaggtt gaggattcgt tctcccgctc gatcccatat gaccaggtcc
    22201 ctcgggttcg tggcctcggg actcgaatat tcggggtggg cgtggtccac gtagaatcgc
    22261 gccccgttgg agaggatgag gttgatgagg cgggactcct ccttggacgg gacgtctttc
    22321 tcctccatgt actcgaagcc acgggcgtcg cgcagcgggc tctccgcctc gtagtcccag
    22381 cggatgcgcg atgaccggta ggtctcgtag gagttgatca gcagcaggga ggacaggatc
    22441 gggttgaagt ccggctggtt cttgaccgtg atcccgtact ccgtctcgat gcccatgact
    22501 ttcgggatcg ccattcgccc ctcgcctctc acgctcggcg cttatggtac agacacaaac
    22561 ccccggtgtc gcgcctcgcg cgaaccgggg ggaaatgggg ggcgagagcc cccccattcc
    22621 ttacaggtac tggccggtgg tgaccacccg ctcgacgctc ttctgcttgt ccctggcgtc
    22681 gctcatgagc ggcttcacgt agacgatccg ctcgcccttc ttgccggcga tcttcgccca
    22741 gtcgtcgggg ttggtcgtgt tgggcaggtc ctcgttctcc ttgaactcct cgcgcaccgc
    22801 ggcgacgagg tcgtcctcgt tgagcccctt gccgccgccg gcgatgtggc gcttgagcgc
    22861 catcttcttg gcccgccgca ccaccgactc gatcatggcc cccgacgaga agtccttgaa
    22921 gtagaggacc tccttgtcac cgttggcata ggtgacctcc aggaaccggt tctcctcggt
    22981 gagctggtac atcgactcca ccgccgccga gatcatcgcg tccaccgcgg actgcgcgct
    23041 gccgtgcctg cggatctccg gctcggcgat cggcaggtcg gtggtcaggt acttgtggaa
    23101 gatgtcgctg gccgcgtcgc ggtcgggacg ctcgatcttg atcttcacgt ccaggcggcc
    23161 gggtctcagg atcgccgggt cgatgaggtc ctggcggttc gaggcgccga tgacgatgac
    23221 gttgcgcagc ccctcgacac cgtcgatctc ggccaggagc tggggcacga tcgtggtctc
    23281 gacgtccgag gagatcccgg tgccgcgcgt ccggaagagc gcgtccattt cgtcgaagaa
    23341 caccacgacc ggcacgtcgt cagcggcctt ctccttggcc ttcgtgaaga tctcgcggat
    23401 cttgcgctcg gtctcgccca cgtacttgtt caggagctcg gggcccttga tgttcaggaa
    23461 gtagcccttg atcttctcgc cgcgcttctc ggagatcttc tcggcgaggt tgttggccac
    23521 ggccttggcg atcatggtct tgccgcagcc gggaggcccg tagagcagga cacccttggg
    23581 cggtgtgagc ttgtgctcct tgtagtagtc ggcgtagagg tagggcagct ccacggcgtc
    23641 cttgatggcc tcgatctgcg tgacgaggcc gccgatgtcg tcgtagccga tgtcgggcac
    23701 ttcctcgagc gcgaggtctt cgacctcgct cttgggcagg cgctcgagca ggtagccgga
    23761 cttcgcgtcc atgaggatgt gatcgccggt cttgagcctg gtggtgcgga gcggatcggc
    23821 gatgataccg accttctcct cgtccgcccg gagcgtcacc acggcgcgct cctcgtcgag
    23881 ctgctccttg aggatgacga cgtcgccctg gatctcgtag cccgccgcct ccaccacgtt
    23941 gaggccctcg ttgaggacca gctcctgccc gggccgcagc gcctcggcct tgatggcggg
    24001 gtggaggttc accttcacct tgcggccctg ggagaggatg ttgaccgtgg cgtcctcgtt
    24061 gaccgagagg tacacgccgt aggtcgacgg cggtgcgcag agcttgtcga cctcctcctt
    24121 gagcgacgtg atctgctcgc gggcttcgta gagggcgttc accagcttct cgttctggtt
    24181 gaaggcctgc accagctgac ggttcgcctc gcgcagcttg ttctcgagga ccatgaactc
    24241 cttgggcatg tcctcgagct tcttgcggag gtggacggtc tcggcctcga gcgaacgcac
    24301 gtacgcctgc aggtcctgca cctggatttc gtactccgtg agccggcgac gcgtgccgga
    24361 gtccgacatg gacgcgggga ggaacttcga aaaaccttcg cgacccgggc tacccttctt
    24421 ctcgaactcc ttcatagcct atcccttcta tcccgacccc aggtgcgccg cagtggcatg
    24481 atcaccgctc gtgatcgagt atggcgacag cgtccgagaa agtcaaggtt caggcccctt
    24541 tacatcgtct ctcccgcgtg cctcgcacgc cgctcgacaa ccctcttcag caagttccgt
    24601 accgcggtcc ttcgcttgcc ttcgttgaca tcgaaacgag tggcgttgaa gcggggttcc
    24661 accgtataag aggtcaatgc cgacgccgcg ctacgtcggt gcgctcggaa tcgcgctggc
    24721 aatttttgcc gcatcgttga cgtctctttc cgcccagccc cctcaaccgc tgcgccgcga
    24781 ggtccgcctc ggcgcgcccg gtctccccgg cgccatcgag ccggtgacgg ccctcgaagg
    24841 ggccgccgcg gtgatcgcgc gtcaggtctt cgacacgctg atgaccttcc gtgagtccac
    24901 cacggacgtg gaaccgtcgc tggcgacgcg atgggtcgtc tcgcgtgacg ggctcgagtg
    24961 gagctttacg atccgtgagc gcgtgcggtt ccacgacggt accccgctca cggccgcgac
    25021 ggtggtcgcg agcttcgacc ggcacctcaa ggccgccgcg cgctccacgc cgaccgcctg
    25081 ggcgtcgctg ctccgcggcg cgcccggggt ggtcaaggag gtgcgggccg tagacgtccg
    25141 gaccgtgcag ttcaccctgg cgcagcccta cgcggccctc ctgacgctcc tcgcgcaccc
    25201 ggccttcggc gtggcgcgcg aggtctcggg cgccgacggc gtttcgcgcc tggtcggcac
    25261 cggaccctac cgtgtcaccg aggcgtcggc gggccgaatc gtcatcgacg cggtgccggg
    25321 ccactgggcg ggacagccac gcgccgagcg gctggtgttc ctcgaggtcg gcgacgacga
    25381 ccacgctgag gccgagtttg ccgcgggcgc cctggacgtc tggtttcctg tgacagcgcc
    25441 gcgtcgctcg cagtgggcgc tgtccacgcc gggaccgcgc gtgggctacc tcgccttcca
    25501 gacggagaag gaaccgttct cccgtaagaa ggtccgacgg gccatggccg cggccatcga
    25561 tcctgccgtc ctcggcgtgg cgctcggcag cggcgccgtc cccctccagt cgttcctgcc
    25621 ggccggcgtc tgggcacggc gcgagggctc accggtgctc ggtggcacgc gcaagcaggt
    25681 ggcggcgctg ctcgccgagg ggcagtggcc caagggcttc acgccgacgc tcgtggcgcc
    25741 gaccgagagt ggcagcgtcg acctcaaggc ggtggcggag gcgctccagc gcaccctgca
    25801 ggagagcgat gtcccggtca cgctgcgggt ggaggccgag cccgcggcgg ccgaggcgcg
    25861 gcgagcgggc gaccacgaca tggcgatcgc cgaggccagt gtcgccggcg gcgacccgca
    25921 cctgttcctc tatccgctgt ccacgagcga gggagcgagc aagggagcgc agaccctcaa
    25981 cttctccttc taccggaacc cgcgcctgga cgacatgctg atccgcgcca gccagctcgc
    26041 ctatcggccc gagcgccagc ggttgtacca gcgggcgcag gcgatcctcg ccgaggagat
    26101 gccgtgggtg ccgctctacg tgaagctcct gtgggccgtg gcgcggcccg aggtgcgcgg
    26161 cctcaggcta cacccctcgg gcttccaccg gctcacgagc ctgggtctcg aaggctaacc
    26221 aggccggctc tcggacaggc ctcgagggcg gcggcgaagc cggcgctcga accacgttca
    26281 ctcggacacg ccgctacggg ttgaagaggc gggtcacggc catgccgggg cgcgccgaag
    26341 ggttggggtc c
//
LOCUS       DATIAZ010000170         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_44547, whole genome shotgun sequence.
ACCESSION   DATIAZ010000170 DATIAZ010000000
VERSION     DATIAZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44547"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VLF19_06060"
     CDS             complement(<1..354)
                     /locus_tag="VLF19_06060"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE92850.1"
                     /translation="MVLALAVFTFFVLALPTVPRADAGGPTEQLRAQVDRAFALLEDP
                     GLRVPERAAERRRQLRRIAEQTFDFAAAARRALGRHWEGRTEGQRAEFVRLFTELIDR
                     GYLSKLDLYEGERLAW"
     gene            complement(483..1388)
                     /gene="ftcD"
                     /locus_tag="VLF19_06065"
     CDS             complement(483..1388)
                     /gene="ftcD"
                     /locus_tag="VLF19_06065"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HSE92851.1"
                     /translation="MTRALVECVPNVSEGRDRARVEAFAAAVRATPAVTLLDVHADPD
                     HHRAVYSFAGAPPAVEAGALALATCVIDALDLRTHSGVHPRIGALDVVPFVPLAGCTM
                     AEAVALARRVGRAVAARHGLPVYYYGHAATRPGRRRLPDVRRGGYEALPTRLASGDDP
                     PDDGPPRFDPRSGAVLVAARELLVAFNVWLGSSDLEAARAIARRVRESGGGLPAVQAL
                     GLPLPSRGLVQVSMNLLDFRVTPIPAAFDLVCEEAARRSIGVRQSELVGVAPRAAFAG
                     RSPESVGLAAFRPSLLLDTFVAPSG"
     gene            1415..>2350
                     /locus_tag="VLF19_06070"
     CDS             1415..>2350
                     /locus_tag="VLF19_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HSE92852.1"
                     /translation="MSVLPIGKLSAPVLQALLDRHTVKDPRVVVGPRVGEDAAVIDLG
                     DRYLVATADPITFAADDLGWYALQVNANDVAVRGARPRWFLATLLLPEGRTTDESVGR
                     LFDQLGEACAELDVALVGGHTEVTYGLDRPIVAGAMLGEVAKDRLVTTGGARVGDVVL
                     MTKGVPLEGAAIIAREREADLLARGVPAAVVARARRLLRAPGLSVVPEAELAMELAEV
                     HAMHDPTEGGIATALRELADAAGVGLRIDADRIIVLPEGRALCAAFGLDPLGTIASGA
                     LLLTMSPADAATVIHALAREAIDGHYIGQVVPREKG"
BASE COUNT          325 a          856 c          890 g          279 t
ORIGIN      
        1 ccacgccagg cgctcgccct cgtaaaggtc gagcttcgag aggtatcccc gatcgatgag
       61 ctcggtgaag agccgcacga actccgcgcg ctggccctcc gtccggccct cccagtggcg
      121 gccgagcgcg cgccgggctg ccgcggcgaa gtcgaaggtc tgctccgcga tgcgccggag
      181 ctggcgccgg cgctccgcgg cacgctccgg gacgcgcagc cccgggtcct cgagaagggc
      241 gaacgcgcgg tcgacctggg cgcgaagctg ctcggtgggc ccaccggcgt ccgcgcgggg
      301 gacggtgggc aacgccagca cgaagaaggt gaatacggca agggctagga ccatgcgcgc
      361 ctccgacggc gacgagtcga gccgaagacg aattgtgcaa gcgcggtgcc ggatgacatg
      421 ccacggatgt cacccggcga cggggacgtt gctactggga ctggcgaatg tggtgcaggc
      481 cgctatccag acggcgccac gaaggtgtcg agcaagaggc tcggacggaa ggcggcgagg
      541 ccgacactct cggggctgcg gccggcgaac gccgcgcgtg gcgccacgcc gaccagctcc
      601 gactgccgca cgccgatcga ccgtcgggcc gcctcctcgc acacgaggtc gaacgccgca
      661 gggatggggg tcacccggaa gtcgaggagg ttcatcgaaa cctgcacgag accgcggctc
      721 ggcagcggca gtcccagcgc ttgcacggcc ggcagtcccc cccccgactc gcgcacgcgg
      781 cgcgcgatcg cgcgcgcggc ctcgaggtcc gaggagccga gccagacgtt gaacgcgacc
      841 agcagttccc tcgctgcgac caggacggcg cccgacctcg ggtcgaagcg cggcggcccg
      901 tcgtcgggcg gatcgtcgcc cgacgccagg cgtgtcggga gcgcctcgta gccgccacgg
      961 cggacgtcgg gcaggcgccg gcgcccgggc cgcgtcgcgg cgtggccgta gtagtagacc
     1021 ggcaggccgt ggcgggcggc cacggcgcgg cccacgcgcc gcgcgagggc cacggcctcc
     1081 gccatcgtgc atccggcgag cggcacgaac ggcaccacgt cgagcgcgcc gatgcgcggg
     1141 tggacgccgc tgtgggtgcg caggtcgagg gcgtcgatca cgcacgtcgc cagcgcgagc
     1201 gccccggcct ccacagcagg cggcgccccg gcgaacgagt acacggctcg gtggtggtcg
     1261 gggtccgcgt ggacgtccaa cagcgtgacg gcgggcgtcg cccggaccgc ggccgcgaac
     1321 gcctcgacgc gggcgcggtc gcggccctcg ctgacattcg ggacgcactc gaccagagcg
     1381 cgcgtcacgt ccggtatact agcggtccag cacgatgagt gtcctgccca tcgggaagct
     1441 ttcggcgccg gtgctccagg cgctgctcga ccgccacacc gtgaaggatc cgcgggtcgt
     1501 cgtggggccg cgggttggtg aggacgcggc cgtcatcgac ctcggcgacc gctacctcgt
     1561 ggcgaccgcc gatcccatca cgttcgcggc cgacgatctc ggctggtacg cgctccaggt
     1621 gaacgccaac gacgtggccg tgcgtggcgc gcgcccccgc tggttcctcg ccacgctgct
     1681 cctccccgag gggcggacga ccgacgaatc cgtgggccgc ctcttcgacc agctcggcga
     1741 ggcctgcgcc gagctcgacg tcgcgctcgt cggcggccac accgaggtca cctacggcct
     1801 cgaccggccg atcgtcgccg gcgccatgct cggcgaggtg gccaaggacc gcctggtgac
     1861 gacaggcggc gcgcgcgtgg gcgacgtcgt cctcatgacg aagggtgtgc cgctggaggg
     1921 tgccgcgatc atcgcgcgcg agcgggaggc ggacctgctg gcgcgcggcg tgcccgcggc
     1981 cgtcgtggcg cgcgcccggc gcctgctccg ggccccgggg ctcagcgtgg tgccggaggc
     2041 ggagctggcg atggagctgg ccgaggtgca cgcgatgcac gatccgaccg agggcggcat
     2101 cgcgacggcg ctccgggagc tcgccgacgc tgccggcgtc ggcctccgca tcgatgccga
     2161 ccggatcatc gtgctgcccg agggccgcgc gctgtgcgcc gccttcgggc tcgacccgct
     2221 cggcacgatc gcctcgggcg cgctgctcct gaccatgagc cccgccgacg ccgccacggt
     2281 gatccacgcc ctcgcgcgcg aggcgatcga cggccactac atcggacagg tggttcctcg
     2341 cgagaagggc
//
LOCUS       DATIAZ010000171        10892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_964429, whole genome shotgun sequence.
ACCESSION   DATIAZ010000171 DATIAZ010000000
VERSION     DATIAZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964429"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VLF19_06075"
     CDS             complement(<1..203)
                     /locus_tag="VLF19_06075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE92853.1"
                     /translation="MDLVNVFAHRGQFFRVLQETGRSQTAVMTIAPGADAGPEEEHEG
                     DQIVFVIEGEAVVRVADREYRAGP"
     gene            complement(271..930)
                     /locus_tag="VLF19_06080"
     CDS             complement(271..930)
                     /locus_tag="VLF19_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSE92854.1"
                     /translation="MQSRFAVLAALVVVSAMGAGCATKGWVRETVGKERTQTDSQIAG
                     VEGRVGLEAKRAEQTTARVGEIERSVEGFGRATQAAQERADAAFARADQTDQRLTRLW
                     SKRHERQQVETLDVLFGFDRADLDDGAQTALLNIVRELRANQNLSVDLTGYADPAGAA
                     EYNVRLSQRRVESVRRFLVQRGIELPRINSVGMGVLPDAGVPNDKKRRVTLRLMVAAA
                     E"
     gene            complement(1116..1472)
                     /locus_tag="VLF19_06085"
     CDS             complement(1116..1472)
                     /locus_tag="VLF19_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92855.1"
                     /translation="MIRAAVVTLAALLLAGVLSVAVARLLGRSGSRASLLVSVLAQWL
                     GAYAVWTFAGGLALRSGALSAYDGTLFVLLALVLGFWQYRVRVTSGPERALMVFVTGQ
                     IAWLVVVAAQNGLFVP"
     gene            1690..2562
                     /locus_tag="VLF19_06090"
     CDS             1690..2562
                     /locus_tag="VLF19_06090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HSE92856.1"
                     /translation="MTGFGRGEATGDTITLSVEIRSVNHRHLDLALRLPSVLAPLELD
                     ARRLVQARVERGRVDVGVQPAPAGSQALQDVRVNQALARRYADQARQLGRELGLAGAP
                     SLTWVLERPGVLEIADVTPPPPAVAWPLLERALAGALDELVARRAAEGAALAAALRGL
                     LTDLGREVDAMATRAPLASARRGERLRERLRSLLGEVPLDESRIATEVAVWAQKTDVA
                     EELARLRAHLDEFAIMLDKGGPVGRPLDFLIQELNREVNTVAAKADDLELSQAALAAK
                     GVIERIREQVQNLE"
     gene            2602..3228
                     /gene="gmk"
                     /locus_tag="VLF19_06095"
     CDS             2602..3228
                     /gene="gmk"
                     /locus_tag="VLF19_06095"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSE92857.1"
                     /translation="MRRRGALVVVSAPSGAGKTTLCREARLRLPDLAYSVSYTTRPPR
                     PGEIEGTDFCFVSEARFRELRDRGAFAEWATVHGNLYGTHAGVLEEALGAGRDILLDI
                     DTQGATQLRRRYQEAVLIFIVAPSMAELEQRLRERRSDAEREIARRLTRAREEIGMWR
                     QYDYLVVNRDLKEAADQLTAIIVAERSRTKRLALGLPDLTISDTEVSR"
     gene            3228..3443
                     /gene="rpoZ"
                     /locus_tag="VLF19_06100"
     CDS             3228..3443
                     /gene="rpoZ"
                     /locus_tag="VLF19_06100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSE92858.1"
                     /translation="MAFPSLENALNKISNRYLLVVLSAKRARQINRGAPPRVESKQKK
                     PTSVALEEISQAKVEYRVKDEGETAKA"
     gene            3443..4672
                     /gene="coaBC"
                     /locus_tag="VLF19_06105"
     CDS             3443..4672
                     /gene="coaBC"
                     /locus_tag="VLF19_06105"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSE92859.1"
                     /translation="MTLDGRELILGVTGSIGAYKAVYLLRELVRRGARVTVVLTEHAG
                     RFVGPLTFRTLSGRPVLADLFDPQSEEAVEHVALAERAHALVVAPATANVLAKAAHGV
                     ADDFLTTLLLAARGPVLMAPAMDGGMWDHPAVVENVATLRRRGVTVLEPDAGALASGL
                     RGKGRLPEPEAIVEALEVLLHPVRDLAGEHVLVTAGPTREPIDPVRYISNRSSGKMGY
                     GVAAAAIRRGARVTLVSGPTALTPPAGAVFVPVQTAEEMREAVLHHLGVATIVVKAAA
                     VGDYRVKQIAAEKIKGKHDLTLDLVANPDILAEVGARRTGAFVVGFAAETTDVVAHAR
                     AKLAAKGVDLLVVNDVSRPDIGFEADDNQVVLIDRWGGERALPRMPKSAVADETLSHV
                     LALRGAARTDRGDRVKR"
     gene            4750..5358
                     /locus_tag="VLF19_06110"
     CDS             4750..5358
                     /locus_tag="VLF19_06110"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSE92860.1"
                     /translation="MTDLPLSDDYLLGPAEALARQERQLQGCRRCKLCEGRTNIVFGS
                     GNPRAELVVIGEGPGADEDVQGKPFVGRAGQLLTKMLESVQLARDQVYICNAVKCRPP
                     GNRNPEPDELTACAPFLAAQFGVLQPKVVLALGSVATQALLRTKEPIGKLRGRVHAYG
                     GAVLIPTFHPAFLLRNPGQEYKRMAWEDLKLAKREYDVRRTR"
     gene            5420..7348
                     /gene="priA"
                     /locus_tag="VLF19_06115"
     CDS             5420..7348
                     /gene="priA"
                     /locus_tag="VLF19_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSE92861.1"
                     /translation="MTIAEVAFDAPLPHPFSYRVPEGWPVSRGQRVAAPLRGAVRVGV
                     VVSVREGDGADLKPLHHCVDPAPLLGAGELALAGWIAAESLTPLGSTCLALLPPAPVG
                     GRPVAGSRSVSRSGSPGEHPSADRPGAASYGVADRTAPAAGRVPVRAVPGTPPVLPEV
                     LVGAGREARLLDAIAREGSSALVLTADVDGAGRWAQRLARLGTCVRLDSGVTEVERAA
                     AWASLAAGDARLAVGTRSALLVPLPPGGTLALVDEHEAAHKPPGPPRVHARDVVLERV
                     ARGGVHALMTSATPSVETWWRVSDGRIAMAELPAAPWPAVTLADTRGIARREALTPPL
                     ARAIRETLATGRRVFLAVSRLASALGCDECGGILKCEQCGIALAYSPAGRTLACRFCA
                     AAAPLPETCPACRGRRLAPFGWGAERVEHAVRRRFAQARIARYDPDAARGRRAEAQRA
                     AAAAAAVVIGTRGALRLFGPASLGLAAFVSPDQLLGLPDFRAAERAFALAWAAAERVR
                     PDGAVVIQTQNPSHYAFAAVAKQDLESFYAPELRFRAELGYPPFRRLAIITAGGADAA
                     AAQRRADDVVGALSGGGLTVYPPAPDRRNRARRVVVKGGPELPRALEDGLRGLRAPRA
                     GARRGIMDVEVDPVEWPS"
     gene            7336..7860
                     /gene="def"
                     /locus_tag="VLF19_06120"
     CDS             7336..7860
                     /gene="def"
                     /locus_tag="VLF19_06120"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSE92862.1"
                     /translation="MAVLKVRRYGDPALRRRAETVGEITPAVRKAVEDMIETMYEEVG
                     IGLAAPQVGIPLRLIVVADDETRHARALVNPVITEQGGQATAEEGCLSIPGVYAPVTR
                     AAWVKVEARDLGGDPVSIDARGLLARVLQHELDHLDGVLFIDRLDPVMRDRLKRRIKK
                     EGLPEDARHHAFAL"
     gene            7882..8820
                     /gene="fmt"
                     /locus_tag="VLF19_06125"
     CDS             7882..8820
                     /gene="fmt"
                     /locus_tag="VLF19_06125"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSE92863.1"
                     /translation="MRVLFYGTPEFALPTLEALLQRHHVVAAVTQPDRPAHRGQRLTP
                     PPVKVRALAAGLPVLQPARLRDPEWPERLRALGADVAVVVAFGQILPKAVLDAPPRGS
                     INVHASLLPRYRGAAPIAWAIMRGETVTGITTFQMDEGMDTGPMLLRAETPIGPHETA
                     GELAARLAVLGARVLTDTLERLDALTPEPQRHEQATRAPRLTKTDGYLDWHRAARELC
                     NVVRGCNPWPGAAAASLAGRLTIWRARSVGSDRLDPPGTLAPWDGEIVIATPEGGVLP
                     LEVQPESKRRMAWPEYLRGARLGIGARFLRLRLTEP"
     gene            8817..10157
                     /gene="rsmB"
                     /locus_tag="VLF19_06130"
     CDS             8817..10157
                     /gene="rsmB"
                     /locus_tag="VLF19_06130"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HSE92864.1"
                     /translation="MSAASAARAAAATVLERVLVDGAFADLALDAEFRRRPLSVRDSA
                     LATEIVFGTLRWQRYLDWVLGPHSRRPIETLDPRALVLLRMTAYQIAFLERVPPFAAV
                     NDAVTLARGGRAKPGVSGFVNAVLRSFARRRAREREPRPPADPVEALALRCSFPTWLA
                     ARWVARWGPAEAEALMRAMNERPPLTLRANTLRMTREALAARLAAVDGVVTQPTRYAP
                     EGLVAGHVGRPADWRAFADGAFAVQDEASMLVARLLDPRAGETVADVCAAPGTKTTHL
                     AQLMDGRGRILAFDPQPARLALVTEAAARLGVTIVEALGGTVEALAPRFPGACDAVLV
                     DAPCSNLGVLRRNPEVKWRRGPDVIAAGSVRQRAILDAAAAMVRSGGRLVYATCSLEP
                     EENEEVVRDFLAARRDFALAPPGVFPLPLDGGVLRCLPHHHGVDGFTAVRLRRV"
     gene            10422..>10892
                     /locus_tag="VLF19_06135"
     CDS             10422..>10892
                     /locus_tag="VLF19_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HSE92865.1"
                     /translation="MTYPVLKFVHLLGLILMGAGLIGVWYADLRSRQVRELPVFAEAV
                     RSIAVLYDGLVVPGALLLLGSGTWLIVTVYGGWAFLGMPWLSGMVVLFAFEFIEGNTI
                     TRSYFMRLRRLTMEAIQQGRVTAELAEARRHVPTFAHFLDIPILFVIVALGAMRP"
BASE COUNT         1423 a         4056 c         3908 g         1505 t
ORIGIN      
        1 ggtcccgcgc gatactcgcg atcggccaca cgcacgacgg cctctccttc gatcacgaac
       61 acgatctgat cgccctcatg ctcttcctcc gggcccgcgt cggcgcccgg ggcgatcgtc
      121 atcacggccg tctggctgcg cccggtctcc tgaagcacgc ggaagaactg gccccggtgg
      181 gcgaacacgt tcacgagatc catgacgcgc ctcctcgcgg cgccccgggc ggggcgccgc
      241 gaggagagcc taccgcagag ggctggcgat ctactctgcc gccgcgacca tcagccgcaa
      301 cgtgactcga cgcttcttgt cgttcgggac gcccgcgtcc ggcagcacgc ccatcccgac
      361 ggagttgatt ctcggtagct cgatgccccg ctggacgagg aagcggcgca ccgactccac
      421 tcgacgctgg ctcagccgca cgttgtactc ggccgcgccg gccggatcgg cgtaaccggt
      481 gagatccacg ctgagattct ggttcgcccg cagctcgcgg acgatgttga gcagcgcggt
      541 ctgcgccccg tcgtcgaggt ccgcgcggtc gaacccgaac aggacgtcga gagtctcgac
      601 ctgctgccgc tcgtggcgct tcgaccagag gcgcgtcagc cgctggtcgg tctggtccgc
      661 acgcgcgaac gccgcgtccg cgcgctcctg cgccgcctgc gtcgcgcgac cgaacccctc
      721 gaccgagcgc tcgatctcac caacgcgtgc ggtcgtctgc tccgcccgct tcgcctcgag
      781 gccgacgcgt ccttccacgc cggcgatctg actatccgtc tgcgtccgtt cctttcccac
      841 cgtctcacgc acccagccct tggtagcgca tccagcaccc atcgcggaca ccacgacgag
      901 cgcggccagc actgcgaatc ggctctgcat aagaactcct cctggtcgac gtttaccggg
      961 ctccgatgca actcggccgc catatgtcgg gcgagggctc cgaccgctcg gtaagagccc
     1021 gccaaatctg gccggcgccg tgcccgcagg agccgcacga ggccggctcc gcgacctgcg
     1081 agtcggcggg atgacgcttc cgacgggccc gtccgtcagg ggacgaacag cccgttctga
     1141 gctgcgacga cgacgagcca ggcgatctgg ccagtgacga agaccatcag ggcccgctcc
     1201 ggccccgacg tgacacgcac ccggtactgc cagaatccca gtacgagggc cagcagaacg
     1261 aacagcgtcc cgtcgtacgc gctcagggcg ccggagcgca gggcgagacc gccggcgaag
     1321 gtccacacag cgtaggcgcc gagccactgc gcgagaaccg agaccaggag cgacgcgcgg
     1381 gagcccgagc gcccgaggag cctcgcgacg gcgaccgaca ggaccccggc gagcaggagg
     1441 gccgccagcg tgacgacggc ggcgcggatc acctccgggc ccggtgctct ggacgcccgc
     1501 ggagcatgcg cccctcggca cgctcgacca ggtgctgacc tcctccttta cgggtcggga
     1561 atgcaagtct acgcttcggc cgagggtgtg tcaacgcaac acattgacgt tacaggcgat
     1621 ttccgtctat catctgtcga tgagcgccgc gtccgccgga gcccctgggt cccttcgatg
     1681 atccgcagca tgacggggtt cgggcgcggc gaggcgaccg gcgacacgat caccctcagc
     1741 gtcgagatcc ggtccgtcaa tcaccgccac ctcgacctgg cgctccgcct gccgtccgtg
     1801 ctggcgcccc tcgagctcga cgcgcgccgg ctcgtccagg cccgcgtcga gcgcggacgc
     1861 gtggacgtcg gtgtgcagcc ggcgccggca ggcagccagg cactgcagga cgtccgcgtg
     1921 aaccaggcgc tcgcgcgacg ttacgccgat caggcgcggc agctcgggcg cgagctgggc
     1981 ctcgccggcg cgccgtcgct cacgtgggtg ctggagcggc ccggcgtgct cgagatcgcc
     2041 gacgtcacgc ctcccccacc ggccgtcgcc tggccgctgc tcgagcgtgc gctggccggg
     2101 gcgctcgacg agctggtcgc gcgccgcgcg gcggaaggcg cggcgctggc cgccgcgctg
     2161 cgcgggctcc tcaccgacct cggcagggag gtcgacgcga tggccacgcg cgcgccgctg
     2221 gcctcggcgc gccgcggcga gcggctccgc gagcggctgc gctcgctgct cggcgaggtg
     2281 cctctcgacg agtcgcgcat cgcgaccgag gtggcggtgt gggcgcagaa gaccgacgtt
     2341 gcggaagagc ttgcgcggct gcgcgcgcac ctcgacgagt tcgcgatcat gctcgacaag
     2401 ggtggcccgg tggggcggcc gctcgacttc ctgatccagg aactgaaccg ggaagtgaac
     2461 acggtcgccg ccaaggcgga cgacctcgag ctgtcccagg ccgcgctcgc cgcgaagggc
     2521 gtgatcgaga ggatacggga acaggtgcag aaccttgaat agacgggccc cggccggcgg
     2581 ccatcgcgac ggagccgcgt gatccgccgg cgcggcgcgc tcgtcgtcgt ctccgcgccc
     2641 tcgggcgccg gcaagacaac gctctgccgc gaggcgcggc tccggctgcc cgacctcgcc
     2701 tactcggtgt cctataccac gcggccgccg aggccgggcg agatcgaagg cacggacttc
     2761 tgcttcgtga gcgaggcgcg gttccgcgag ctgcgcgacc gcggcgcgtt cgccgagtgg
     2821 gcgaccgtcc acggcaacct ctacggcacc cacgccggcg tgctcgagga ggcgctcggc
     2881 gccgggcgtg acatcctgct cgacatcgac acccaggggg ccacccagtt gcggcggcgc
     2941 taccaggagg ccgtgctgat cttcatcgtg gcgccctcga tggccgagct cgagcagcgc
     3001 ctgcgcgagc gccgctccga cgccgagcgc gagatcgcgc ggcgcctgac gcgcgcccgc
     3061 gaggagatcg gcatgtggcg ccagtacgac tacctcgtcg tcaaccgcga cctcaaggag
     3121 gccgccgatc agctcacagc gatcatcgtg gccgagcgct cgcgcaccaa gcgcctcgcg
     3181 ctcggcctgc cggatctcac gatttccgac acggaggtca gccgctgatg gcattcccgt
     3241 cactggaaaa cgcgctcaac aagatctcga accgctacct gctcgtcgtc ctctcggcca
     3301 agcgggcgcg ccagatcaac cgcggcgcgc cgccgcgcgt ggagagcaag cagaagaagc
     3361 ccaccagcgt cgcactggag gagatctccc aggccaaggt ggagtaccgc gtgaaggacg
     3421 agggcgagac cgcgaaggcg tgatgacgct cgacggccgc gagctgatcc tcggcgtcac
     3481 cgggtccatc ggcgcctaca aggccgtgta cctgctgcgc gagctcgtgc gccgcggcgc
     3541 gcgggtgacg gtcgtcctca ccgagcacgc cgggcgcttc gtcgggccgc tgacgttccg
     3601 gacgctctcc ggccgtccgg tgctggcgga tctgttcgac ccgcagagcg aggaggccgt
     3661 ggagcacgtg gcgctcgccg agcgcgccca tgcgctcgtc gtggcgccgg cgacggcgaa
     3721 cgtgctggcc aaggccgccc acggtgtcgc ggacgacttc ctgaccacgc tgttgctcgc
     3781 cgcccgcggc ccggtgctga tggcgccggc catggacggc ggcatgtggg accatccggc
     3841 cgtcgtcgag aacgtggcga cgctccggcg tcggggcgtc accgtgctcg agcccgacgc
     3901 cggcgcgctg gcctcgggac tcaggggcaa gggccggctg ccggagcccg aggcgatcgt
     3961 cgaggcgctg gaagtcctgc tccaccccgt gcgcgacctg gccggcgagc acgtgctcgt
     4021 caccgccggc cccacccgcg agccgatcga tcccgttcgt tacatctcga accgctcctc
     4081 cggcaagatg ggctacggcg tcgccgccgc cgccatccgg cggggcgcgc gcgtgacact
     4141 cgtgtcgggc ccgaccgcgc tcacgccgcc ggccggcgcc gtgttcgtgc ccgtgcaaac
     4201 ggcggaggag atgcgcgagg cggtgctgca tcacctcggc gtcgccacga tcgtcgtcaa
     4261 ggccgccgcc gtcggcgact atcgcgtcaa gcagatcgcc gccgagaaga tcaagggcaa
     4321 gcacgatctc acgctcgatc tcgtggcgaa tcccgacatc ctcgccgagg tcggcgcgag
     4381 gcgcaccggg gctttcgtcg tcggcttcgc cgccgagacc accgacgtcg tggcccacgc
     4441 ccgggccaag ctcgccgcca agggtgtcga cctgctcgtg gtcaacgacg tgagccgccc
     4501 ggacatcggc ttcgaggccg acgacaacca ggtcgtgctg atcgaccgct ggggcggcga
     4561 gcgcgcgctg ccgcggatgc ccaagtccgc cgtcgccgac gagaccttga gccacgtgct
     4621 cgcgctgcgc ggagcagcgc ggacggaccg cggcgaccgg gtgaagcgct gagcgccgac
     4681 ccccggagcg ccctggccga ggtgctcggc accctcggcg ggattctccg ccatcatcgc
     4741 gacctcggcg tgaccgacct ccccctctcg gacgactacc tgctgggccc cgccgaggcc
     4801 ctcgcgcggc aggagcgcca gctccagggc tgccggcgct gcaagctctg cgagggccgc
     4861 acgaacatcg tcttcggctc gggcaaccca cgggccgagc tggtggtgat cggcgagggg
     4921 ccgggcgccg acgaagacgt gcagggcaag ccgttcgtgg gccgcgccgg ccagctgctg
     4981 acgaagatgc tcgagtcggt ccagctcgcg cgcgaccagg tctacatctg caacgccgtc
     5041 aagtgccggc ctccgggcaa tcggaacccc gagcccgacg agctgacggc gtgcgcgccg
     5101 ttcctcgccg cgcagttcgg cgtgctgcag ccgaaggtgg tgctcgcgct cggcagcgtg
     5161 gccacgcagg cgctgctgcg gacgaaggag ccgatcggca agctccgggg ccgcgtccac
     5221 gcctacggcg gcgcggtgct catcccgacc ttccatcccg cgttcctgct gcgcaacccg
     5281 ggtcaggagt acaagcgcat ggcctgggaa gacctcaagc tcgccaagcg ggaatacgac
     5341 gtccggcgca cgcgatgacg ccggaaggtc cccgcgcgga tcaccctcga ggcgagccgc
     5401 gccggcgacg cccgctgccg tgaccatcgc cgaggtcgca ttcgacgcgc cgctgccgca
     5461 tccgttctcg tatcgcgtgc ccgagggctg gcccgtctcg cgcggccagc gcgtggcggc
     5521 gccgctccgt ggcgcggtgc gtgtcggggt ggtcgtcagc gtgcgagagg gcgacggcgc
     5581 cgacctcaag ccgttgcacc actgcgtcga tcccgcccct ttgctcggcg ccggcgagct
     5641 cgcgctcgcc ggctggatcg ccgcggagag cctcacaccc ctcggctcca cgtgtcttgc
     5701 gctgctgcca ccggcgcccg tcggtggacg gccggtggcc ggctcccgct cggtcagccg
     5761 gtccggatcg cccggcgagc atccgagcgc cgatcgcccg ggtgcggcga gctacggcgt
     5821 cgcggatcgc accgcgccgg cggccgggcg tgtgcccgtc cgcgccgtgc cggggacacc
     5881 gcccgtattg cccgaggtgt tggtcggcgc cggccgcgag gcgcgcctgc tggacgcgat
     5941 cgcacgcgaa ggctcgtccg cgctcgtgct gaccgcggac gtcgacggcg cgggccggtg
     6001 ggcacagcgt ctggcgagac tcggcacgtg cgtccggctc gactcggggg tgaccgaggt
     6061 cgagcgcgcc gccgcatggg cgtcgctcgc tgccggtgat gcccgcctcg ccgtcggcac
     6121 ccgctcggcc ctgctcgtcc cgctgccgcc aggcgggacg ctcgcgctcg tggacgagca
     6181 cgaggcggcc cacaagccgc cggggccgcc gcgcgtgcac gcgcgcgacg tggtcctcga
     6241 gcgggtcgct cgcggcggtg tgcacgcgct catgacttcg gccacgccga gcgtggagac
     6301 gtggtggcgg gtctcggacg gtcggatcgc gatggcggag ctgccggcgg cgccctggcc
     6361 cgcggtgacg ctcgccgaca cgcgcggcat cgccagacgc gaggccctga cgccgccgct
     6421 cgcccgcgcc atccgggaga cgctcgcgac cggccgccgc gtgttcctcg cggtcagccg
     6481 gctcgcgtcg gcgctcggct gtgacgaatg cggcgggatc ctcaagtgcg agcagtgcgg
     6541 cattgcgctc gcgtactcgc cggccggccg gacgctggcg tgccggttct gcgccgcggc
     6601 ggcgcccctg cccgagacgt gcccggcctg ccgcggacgg cgactggcgc cgttcggctg
     6661 gggcgccgag cgcgtggagc acgcggtgcg gcgacgcttc gcgcaggcgc ggatcgcgcg
     6721 ctacgacccc gacgccgcgc gaggccggcg cgccgaggct cagcgggcgg ccgcggccgc
     6781 ggcggcggtc gtgatcggca cccgtggcgc gttgcggctc ttcggtcccg cctcgctcgg
     6841 tctcgcggct ttcgtgtcac cggaccagct cctcgggttg ccggacttcc gcgccgccga
     6901 gcgcgccttc gccctggcct gggcggcggc cgagcgcgtg cgccccgacg gcgccgtcgt
     6961 catccagact cagaacccga gccactacgc cttcgccgcc gtcgccaagc aggacctcga
     7021 gtcgttctac gctcccgagc tgcgcttccg cgccgagctc gggtatccgc cgttccgacg
     7081 gctcgcgatc atcacggcgg gcggcgctga cgcggccgcc gcccagcggc gcgccgacga
     7141 cgtcgtcggc gcgctctcag gaggcgggct gacggtgtac ccgccagcgc cggaccgccg
     7201 caaccgcgca cgccgcgtgg tcgtgaaggg tgggcccgag ctgccgcggg cactcgagga
     7261 cggcctccgc ggcctccgcg cgccgcgagc cggcgccagg cgcggtatca tggacgtgga
     7321 ggtggacccg gtcgaatggc cgtcctgaag gtgagaaggt acggggatcc tgccctgcgg
     7381 cgacgcgccg agaccgtcgg cgagatcacg ccagcggtcc ggaaggccgt cgaggacatg
     7441 atcgagacga tgtacgagga ggtcggcatc gggctggccg caccccaggt cggcatcccg
     7501 ctgaggctca tcgtcgtcgc cgacgacgag acacggcacg cccgcgcgct cgtcaatccc
     7561 gtcatcacgg agcagggtgg ccaggcgacg gccgaggagg gctgtctttc cattcccggt
     7621 gtgtacgcgc cggtgacccg cgccgcgtgg gtgaaggtcg aggcgcgcga cctcggaggc
     7681 gatcccgtca gcatcgacgc ccggggcctc cttgcgcgcg tgctccagca cgagctcgat
     7741 cacctcgacg gcgtgctctt catcgaccgc ctcgaccccg tcatgcgtga ccggctcaag
     7801 cgcaggatca agaaggaagg gctccccgag gacgcgcggc atcacgcctt cgcgctgtag
     7861 acgcgcgcag cggggcgacc ggtgagggtc ctcttctacg gcacgcccga attcgcgctg
     7921 ccgaccctcg aggcgctcct ccagcgccat cacgtcgtgg ccgccgtgac ccagccggac
     7981 cgcccggcgc accgcggcca gcgcctgacg ccgccgccgg tgaaggtgcg cgcgcttgcc
     8041 gccgggctac cggtgctcca gccggcgcgc ctgcgcgatc ccgagtggcc ggagcggctc
     8101 cgcgcgctcg gagccgacgt ggccgtcgtg gtggcgttcg gccagatcct gcccaaggcc
     8161 gtcctcgacg cgcccccccg gggctcgatc aacgtccacg cctctctctt gccccgctac
     8221 cgcggcgcag cgccgatcgc ctgggcgatc atgcgaggcg agacggtcac gggcatcacg
     8281 acgttccaga tggacgaggg catggacacc ggccccatgc tgctgcgcgc cgagacgccg
     8341 atcggcccgc acgagaccgc gggcgagctc gcggcccgcc tcgccgtcct cggcgccagg
     8401 gtgctgaccg acacgctcga gcgcctcgac gcgctcacgc cggagccaca gcgtcacgag
     8461 caggcgacgc gggcgccgcg gctcacgaag accgacggat acctcgactg gcaccgcgcg
     8521 gcccgcgagc tctgcaacgt cgtccgcggc tgcaacccgt ggccgggggc cgcggcggcg
     8581 agcctggccg gccgcctcac gatctggcgt gcgcggagcg tgggctccga ccgtctggat
     8641 ccgcccggca cgctcgcgcc gtgggacggc gagatcgtca tcgcgacacc cgaaggcggc
     8701 gtgcttccgc tcgaggtcca gcccgagagc aagcgtcgca tggcgtggcc cgagtacctg
     8761 cgcggcgcgc gactggggat cggcgcgcga tttctccgtc tcaggctcac cgagccgtga
     8821 gtgcggcgtc agccgcgcgc gccgccgcgg ccaccgtcct cgagcgcgtc ctcgtggacg
     8881 gcgcgttcgc cgacctggcg ctcgacgccg agttccgccg ccggccgctc tccgttcgtg
     8941 acagcgcact ggccaccgag atcgtcttcg gcacgctgcg ctggcagcgc tacctggact
     9001 gggtgctcgg cccccactcc aggcggccga tcgagaccct cgatccccgg gcgctcgtgc
     9061 tccttcgcat gacggcctac cagatcgcgt tcctcgagcg tgtgcccccg ttcgccgccg
     9121 tcaacgacgc ggtcaccctg gcccgcggcg gccgcgcgaa gccgggcgtg agcgggttcg
     9181 tgaatgcggt gctgcgctcg ttcgcccggc gccgcgcgcg cgagcgcgag ccgcgcccgc
     9241 ctgccgatcc cgtcgaggcg ctggccctcc gctgttcgtt cccgacctgg ctggccgcgc
     9301 gctgggtcgc gcgctggggc cctgccgagg ccgaggcgct gatgcgggcg atgaacgagc
     9361 gcccgccgct cactcttcgc gcgaacacgc tccggatgac gcgcgaggcg ctggccgcgc
     9421 gcctcgccgc cgtcgacggc gtggtcaccc agcccacgcg gtacgcgccc gaggggctcg
     9481 tggccgggca cgtcggcaga cccgcggact ggcgggcgtt cgccgacggc gccttcgccg
     9541 tgcaggacga ggcctccatg ctggtggcgc ggctgctcga cccgcgcgcc ggtgagacgg
     9601 tcgccgatgt gtgcgcggcc cctggcacca agaccacgca cctcgcccag ctcatggacg
     9661 gccgcgggcg catccttgcc ttcgatccgc agccggcacg gctggcgctc gtgacggagg
     9721 cggcggcacg cctcggcgtg acgatcgtgg aggctctggg cggcacggtc gaagcgctcg
     9781 cgccgcgctt ccccggcgcc tgcgacgccg tgctcgtgga cgcgccctgc tcgaacctcg
     9841 gcgtgctacg tcgcaacccc gaggtgaagt ggcggcgcgg cccggacgtg atcgccgccg
     9901 gcagtgtgcg ccagcgggcc atccttgacg cggcggcggc gatggtacgc tccggtgggc
     9961 ggctggtcta cgcgacgtgc tcgctcgagc ccgaagagaa cgaggaggtc gtccgcgact
    10021 tcctcgccgc gcggagggac ttcgcgctcg ccccaccggg cgtcttcccc ttgccgctgg
    10081 acggcggcgt gctgcgctgc ctgccccacc accacggcgt cgacggcttc accgcggtac
    10141 ggctgcggcg agtctgaggc gccccggcgg cgtggtgtga ggtgacgcca tcctgaggcc
    10201 cggggagcgt ccgcggtcgt ggtctcgcgg tcgtcgccgc cgcggcgcgg gcggcggcgc
    10261 gatggagcct gctcgcggtc gctcgccgcg cgggtgcatc ggcgtcggtc actcttgctc
    10321 caccccgggg cacgacacta ttctcccgtc accatgggac agtggaacgg acagcggcgt
    10381 gagcggaggg ggctcggtgt gaggccgacg ggggccatac cgtgacgtat cccgtcctca
    10441 aattcgttca tctgctcggc ctcatcctga tgggggcagg cctgatcggc gtctggtacg
    10501 cggatctccg ctcgcgccag gtccgggagt tgcccgtgtt cgccgaggcc gtccgaagca
    10561 tcgcggtcct atacgatggc ctcgtcgttc ccggcgcgct tctgttactc ggctcgggaa
    10621 cgtggctgat cgtcacggtc tatggtgggt gggcattcct cgggatgccg tggttgagcg
    10681 ggatggttgt cctcttcgca ttcgaattca tcgaggggaa cacgatcaca cggagctatt
    10741 tcatgcgcct gcgacgcctc acgatggagg cgatccagca ggggagggtg acggcggagt
    10801 tggcggaagc ccggcgccac gtaccgacct tcgcgcactt cctcgacatt ccaatcctct
    10861 tcgtgatcgt ggcgctgggc gcgatgcggc cg
//
LOCUS       DATIAZ010000172         8679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_175802, whole genome shotgun sequence.
ACCESSION   DATIAZ010000172 DATIAZ010000000
VERSION     DATIAZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175802"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VLF19_06140"
     CDS             <1..388
                     /locus_tag="VLF19_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSE92866.1"
                     /translation="SGLPCCHDEVNLILPGRNYGWPEVYGKGGDARFVDPIVESGLGT
                     WAPSGMAFRGGSLYVGGLRGRALYQVRLTDDGRAAGTARLLADAYGRLRDVVTGPDGA
                     LYVTTSNRDGRGSPAAADDRILRVEP"
     gene            394..1653
                     /locus_tag="VLF19_06145"
     CDS             394..1653
                     /locus_tag="VLF19_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HSE92867.1"
                     /translation="MFQPELEALPRERLEALVLQRMRGTLARLTEHPAWRRRLGSVVP
                     DDLARLEDWRRLPFLTKDDLRDAYPFGLAGGTAYRRVQMSSGTTGNPILNPYTEADVA
                     QWSEVMARCYVAAGVTGADVIQITPSFGLFTGGFGFHYGAERLGAMVVPIGAGRTLLQ
                     LKLMRDLAVTVVAAIATYPLRLIEVAREARFDLGTLALRVGIFGSEMWSDELRARIER
                     DLRIRTFDIIGMTETGGPGLGIDCVARAGIHVWEDHYHVEVVDPVTGRVAADGVEGEL
                     VVSTLTREGLPLVRYRTRDLTRVVSRARCDCGRTALRLDRLRGRTDDMVIVKGVNFYP
                     RQVEQVVLRQPGLSHEYQIVLDGEGGERMTVLVEAEPGCDAAVAARVRRELHDLLALS
                     PEVRLCRPGEIERAQGKAVRVVDRRNP"
     gene            1658..3700
                     /locus_tag="VLF19_06150"
     CDS             1658..3700
                     /locus_tag="VLF19_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSE92868.1"
                     /translation="MPALDALFAPRSVAVLGVSRNPAKLGHRLLQNVKEGGFEGAVHP
                     VNPSGEPVLGYATVPVIEALPEGVDLALVSLPAPAVPAAVEALAARRARAAVVLSSGF
                     GEVDAEGRSAQADLLATARAGGLRLVGPNCMGVYSAPASLNGTYFWDLPRLPGGIGVV
                     SQSGAYGGLIIRHLGARGLGVVRFLSIGNQADVGIAEVIEYLADDPATTLIACFVEAV
                     RDGAAFVVAAERAVERKPVVVLKGGRSDAGRRAAGSHTGALAGSHEAFRAACLRASVV
                     LADETEEFLDAIEALVVADGARPGAPRVAVLTVSGGPSVVAADCAERSGLTVPALPDE
                     TRTALRALLPSFAGVGNPVDLTPQVEPGRIGEAVRLVFDRPEISGAVAVDVGLDVPEF
                     AAAVVAAARATGKPAVAFAADAPRVGEALRAGGVPVLTSPERAVRAWRALWQAGRRRP
                     ARRLARVAALPPDVTAALGAGRGPLPYALARRALEAYGVRFCREAVVATDDEAVAAAE
                     RLGYPVVVKVDATGLVHRSEVGGVRPDVRTAAEVTAACRALGRIAGAERFVVQERVGP
                     GVELLVGARRDPVFGPVVACGAGGMLTEAIRDVTVRLAPTAEGEAESMLEDGVRARLL
                     AGPRGLPPVERSAVVDVIEAVGALIFGQPRIQEIDVNPLIAAGATVVAVDALVILG"
     gene            3854..5002
                     /locus_tag="VLF19_06155"
     CDS             3854..5002
                     /locus_tag="VLF19_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE92869.1"
                     /translation="MRDDFYLTDEQRMIRDLARKVAREKIAPHAADVDERERYPEEAM
                     RALAEAGLFGIWVPEEYGGSDMGCLALTLAAEEVAWACAASATQYVDQPLGGLPILMF
                     GTTEQKKKYLPRLATGDILSAYSLSEPEAGSDAAGLKTTAVRRGDHYVLNGSKQWCTN
                     GDHADVLCVFATVDPSKRARGVTAFLVDRDAPGFGVGKKERKMGIRGSPTVALHFTDC
                     RIPAEQRLGEEGEGFKIAMSILDITRPATGAMAVGIAQAALDAAVGYAKERRQFGQRI
                     ADFQGLQFMLADMAIHTHAARLMVHHAARQVDAGIKGNTYEASMAKCWAGDTAMKVAT
                     DAVQVFGGYGYTREFPVERLMRDAKIMQIYEGTNQIQRMIVARELLGG"
     gene            complement(5055..6890)
                     /locus_tag="VLF19_06160"
     CDS             complement(5055..6890)
                     /locus_tag="VLF19_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSE92870.1"
                     /translation="MPYQPIENYGIIGDLHTVALVGMNGSIDWLCLPRFDSPSVFAAI
                     LDDAKGGRFRITPAYGDVKVKQLYWPDTNVLISRFLSPSGVAEITDYMPVGRRPDRDG
                     YHQVVRRVTAVRGSIPVRLECHPAFNYARDPHETELTSEGAVFRSPSLSLGLATKIPL
                     SRDARGVTADFVLSENESETFVLRQIPAGANAGLGLSDPESDALFEATVDFWRRWLAR
                     CSYTGRWREMVHRSALALKLLTYEPTGAIVAAPTCSLPEGLGGPRNWDYRYTWVRDAA
                     FTLYALLRLGFTDEAARFMEWIEARCHELNPDGSLQIMYGIDGRHTLTEETLDHLEGY
                     RGSRPVRIGNGAYGQLQLDIYGELMDAVYLYNKYGNPISYDFWVHLRRLTNWVCDNWQ
                     RTDEGIWEVRGGQQHFVYSKLMCWVAVDRALRLADKRSFPADRERWSKVRDLMYEEIM
                     ASGWNSRRKAFVQHYGSECLDAANLMMPLVFFLSPTDGRMLGTLDATLEPPERGGLVS
                     NSLVYRYNVREAADGLEGEEGTFNICTFWLVEAMTRAGRHDRGRLDRARLVFEQMLGY
                     ANHLGLYAEETGASGEALGNFPQAFTHLAVISAAYNLDRALRGRA"
     gene            complement(6896..8023)
                     /locus_tag="VLF19_06165"
     CDS             complement(6896..8023)
                     /locus_tag="VLF19_06165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92871.1"
                     /translation="MEFGVFVEQLRRGTSQADAFREMFELADAAETWGLDIFWLAEMM
                     VNPTRSVLSAPLLVASWIAARTRRLRVGTAVQLLPLNHPVRVAGEVATLDHLSQGRFD
                     FGVGRSGGPRAYDALAVPYEESQARFFEALEIILQAWKGEPFSYEGRFYRFSNVTVTP
                     RPYRHPHPPVRMAATTTETFPRVGKMGLPIFVGLRGMDIPELAGCLRMYRQAWHDAGH
                     PGAGDACLRIPIYAAPTETAAREEPHETITYYFQRQADLTRAPIGRAGTGPAERRERQ
                     AERLSGLSYEDILSKKVAFGTGPGLIDRLGRLQEELGRRERDRRRAEPGRAAADGSGD
                     AEPRDPHAAGDARAQMIPPVGTIPRFCRVGRTHVLPCSSTH"
     gene            complement(8262..>8679)
                     /locus_tag="VLF19_06170"
     CDS             complement(8262..>8679)
                     /locus_tag="VLF19_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSE92872.1"
                     /translation="VLQTQEESSRVMASLLASIASVSLLVGGIGIMNIMLVSVTERTR
                     EIGLRMAVGARGRDILTQFLVEAVTLSLIGGAVGVAVGLGGSRAISYFADWRTLVAVE
                     TIVVAFGFSAAIGVFFGFYPARKAARLDPIEALRYE"
BASE COUNT         1194 a         3077 c         3168 g         1240 t
ORIGIN      
        1 gtccggcctg ccgtgctgtc acgacgaggt gaacctgatc ctgccgggac gcaactacgg
       61 ctggcccgag gtctacggca agggcggtga cgcgcgcttc gtcgatccga tcgtggagtc
      121 aggcctcggc acctgggcgc cttcgggcat ggcgttccgc ggcgggagtc tctacgtggg
      181 cgggctgcgg gggcgggcgc tctaccaggt gcggctgacc gacgacggca gagccgcggg
      241 cacggcgcgc ctgctcgccg acgcctacgg ccgtcttcgc gacgtggtca cgggccccga
      301 cggcgccctc tacgtgacca cgagcaatcg cgacgggcga ggctcacccg ccgccgccga
      361 cgatcgcatc ctccgcgtcg agccgtgacc ctcatgttcc agcccgagct cgaggcgctc
      421 ccgcgcgagc ggctcgaggc cctcgtgctg cagcgcatgc gcgggacgct cgcgcgcctg
      481 accgagcacc ccgcgtggcg gcggaggctc ggcagcgtgg tgcccgacga cctcgcgcgg
      541 ttggaggact ggcggcgcct gcccttcctc acgaaggacg atctgcgcga cgcctacccg
      601 ttcggactgg ccggcgggac ggcctaccgg cgcgtgcaga tgtcgagcgg aaccacgggc
      661 aacccgatcc tgaacccgta caccgaggcc gacgtcgccc agtggagcga agtgatggcg
      721 cgctgctacg tggccgccgg cgtcaccggc gccgacgtca tccagatcac gccctcgttc
      781 gggctcttca ccgggggctt cggcttccac tacggcgccg agcggctggg cgcgatggtc
      841 gtccccatcg gcgccggccg cacgctgctc cagctcaagc tcatgcgtga cctcgcggtc
      901 accgtggtcg cggcgatcgc gacctacccg ctgcgtctca tcgaggtcgc gcgcgaggcg
      961 cgcttcgacc tcgggacgct cgcgctgcgc gtcggcatct tcggctccga gatgtggtcg
     1021 gacgagctgc gcgcgcggat cgagcgggac cttcgcatcc gcaccttcga catcatcggc
     1081 atgaccgaga cgggcggccc cggcctcggc atcgactgcg tcgcgcgcgc gggcattcac
     1141 gtctgggagg accactacca cgtcgaggtc gtggacccgg tcaccgggcg cgtggcggcc
     1201 gacggcgtcg agggcgagct ggtcgtctcc acgctgacgc gcgagggcct gccgctcgtc
     1261 cgctaccgca cgcgcgacct cacccgcgtg gtctcccggg cgcgctgcga ctgcgggcgc
     1321 accgcgctgc ggctcgaccg gctgcgcggc cggaccgacg acatggtgat cgtcaagggc
     1381 gtcaacttct accctcgcca ggtcgagcaa gtcgtgcttc gccagcccgg cctctcgcac
     1441 gagtaccaga tcgtgctcga cggtgaggga ggggagcgca tgacggtcct cgtcgaggcc
     1501 gagccgggct gcgacgccgc cgtggccgcg cgcgtgcggc gcgagctgca cgatctcctg
     1561 gcgctcagcc ccgaggtgcg gctctgccgg cccggggaga tcgagcgcgc gcagggcaag
     1621 gccgtccgcg tggtggaccg gcgcaacccg tagcgcgatg cccgcgctcg acgcgctctt
     1681 cgctccgcgc tcggtcgccg tgctgggcgt gtcccgcaat cccgccaagc tcggccaccg
     1741 gctgctccag aacgtgaagg agggcggttt cgaaggcgcg gtgcacccgg tgaacccgtc
     1801 gggcgagccc gtcctcgggt acgcgaccgt gcccgtgatc gaggccctgc ccgagggcgt
     1861 ggatctggcc ctcgtcagcc tgccggcgcc ggcggtgccc gccgcggtcg aggcgctggc
     1921 ggcgcgccgc gcacgggcgg ccgtggtgct gtcctccggc ttcggcgagg tggacgccga
     1981 ggggcgctcc gcccaggccg acctgctcgc cacggcccgc gctgggggcc tccgcctggt
     2041 gggaccgaac tgcatgggcg tctactccgc acccgcgagc ctcaacggca cctacttctg
     2101 ggatttgccg cggctcccgg gcgggatcgg cgtcgtctcg cagagcggag cctacggtgg
     2161 tctcatcatc aggcacctcg gcgcccgcgg cttgggcgtc gtgcgcttcc tctctatcgg
     2221 caaccaggcc gacgtcggca tcgccgaggt catcgagtac ctcgccgacg accccgccac
     2281 gacgctgatc gcctgcttcg tggaggcggt gcgcgatggc gcggccttcg tcgtcgccgc
     2341 cgagcgtgcc gtcgagcgca agcccgtggt cgtgctcaag ggcggccggt ccgacgccgg
     2401 ccgccgcgcc gcgggctccc acacgggcgc gctcgcgggc tctcacgagg catttcgcgc
     2461 tgcctgcctg cgcgcgtccg tcgtgctggc cgacgagacg gaggagttct tggacgccat
     2521 cgaggcgctc gtcgtcgccg atggcgcgcg ccccggcgcg ccgcgtgtgg ccgttctcac
     2581 cgtgtccggg ggcccgtcgg tggtggccgc cgactgcgcg gaacgctcgg gcctgacggt
     2641 gccggcgctg cccgacgaga cgcgaaccgc gctccgcgcg ctcctgccct cgttcgccgg
     2701 cgtcggcaat cccgtggacc tcacgccgca ggtcgagccg ggacgcatcg gcgaggctgt
     2761 gcgactggtc ttcgatcgcc cggagatctc cggcgcggtc gccgtcgacg tggggctcga
     2821 cgtccccgag ttcgccgctg ccgtcgtcgc cgcggcgcgc gccacgggca agcccgcggt
     2881 cgccttcgcc gccgacgctc ctcgagtcgg cgaggcgctg cgcgccggcg gcgtgccggt
     2941 gctcacgagc cccgagcgcg cggtccgcgc gtggcgcgcg ctctggcagg ccgggcggag
     3001 gaggccggcc cgccggctcg cccgcgtcgc ggcgctgccg ccggacgtca ccgcggccct
     3061 cggggccggc cgtggcccgc tgccgtacgc gctcgcccgc cgggcgctcg aggcctacgg
     3121 cgtgcgcttc tgccgggagg ccgtcgtcgc caccgacgac gaggcggtgg cggccgcgga
     3181 gcggctggga tacccggtgg tcgtgaaggt cgacgccacc gggctcgttc acaggagcga
     3241 ggtcggcggc gtgcgaccgg acgtgcggac cgccgcggag gtgacggcgg cgtgccgtgc
     3301 gctcgggcgc atcgcgggcg ccgagcgctt cgtcgtccag gagcgcgtgg gccctggcgt
     3361 cgagctgctc gtcggcgcgc gacgggaccc ggtcttcggc cccgtcgtgg cctgcggcgc
     3421 cggcggcatg ttgaccgaag cgatccgcga cgtcaccgtc cggctggcgc ccacggccga
     3481 gggcgaggcc gagtcgatgc tcgaggacgg cgtgcgggcg cggctgctgg ccgggccgcg
     3541 cgggttgccg cccgtcgagc ggagcgcggt cgtggacgtc atcgaagcgg tgggcgcgct
     3601 catcttcggc cagcctcgca tccaggagat cgacgtcaat ccgctgatcg ccgccggagc
     3661 caccgtggtg gcggtggacg ccctcgtgat cctgggatga ccgcgtcgct cgacgagtcc
     3721 gttccggtgc cctgcctcga ccccgacggc gagcggcgct tccggcgata ctggcgtgtg
     3781 ggcgggccgt gcggcggctc gagccgtccc gccaggctcc gcgcggtcac gcgcgggacg
     3841 gagaggacgg gtcatgcgtg acgatttcta cctgaccgac gagcagcgga tgatccgcga
     3901 cttggcgcgc aaggtggcgc gggagaagat cgcgcctcac gccgccgatg tggacgagcg
     3961 cgagcgttac cccgaggagg cgatgcgcgc gctcgccgag gccgggctct tcggcatctg
     4021 ggtgcccgag gagtacggcg gcagcgacat gggctgcctc gcgctgaccc tggccgccga
     4081 agaggtcgcg tgggcgtgcg cggcgtcggc gacgcaatac gtggaccagc cgctcggggg
     4141 cctgccgatt ctgatgttcg ggacgacgga gcagaagaag aagtacctgc cgcggctggc
     4201 gaccggagac atcctgtcgg cgtactcgct gtcggagccc gaggcgggct cggacgccgc
     4261 cgggctcaag acgacggccg tgcgccgcgg cgatcactac gtgctgaacg gctccaagca
     4321 gtggtgcacc aacggcgacc acgccgacgt cctctgcgtc ttcgccacgg tggacccgtc
     4381 gaagcgcgcg agaggcgtga cggccttcct ggtcgaccgg gacgcgcccg gcttcggcgt
     4441 gggcaagaaa gagaggaaga tgggcatccg cggctcgccc acggtggcgc tgcacttcac
     4501 cgactgccgc atcccggcgg agcagcggct gggcgaggag ggcgaggggt tcaagatcgc
     4561 catgagcatc ctcgacatca cccgcccggc caccggagcc atggccgtcg gcatcgccca
     4621 ggccgcgctc gacgccgccg tcggctacgc gaaggagcgg cggcagttcg gccagcgcat
     4681 tgccgacttc cagggcctcc agttcatgct ggccgacatg gccattcaca cccacgccgc
     4741 gcgtctcatg gtgcaccacg cggcgcggca ggtggacgcc gggatcaagg gcaacaccta
     4801 tgaggcctcg atggccaagt gctgggccgg cgacacggcc atgaaggtgg ccacggacgc
     4861 cgtgcaggtg ttcggaggct acggctacac acgcgagttc cccgtcgagc gcctcatgcg
     4921 cgacgccaag atcatgcaga tctacgaggg caccaaccag atccagcgca tgatcgtcgc
     4981 gcgcgagctg ctcgggggct gagctcccgg ccgccgcgag gcggtgcggg cggcgggtgc
     5041 gccgcgccgc cgcgctacgc gcggccgcgc agcgcgcggt ccaggttgta ggcggcgctg
     5101 atcacggcga ggtgagtgaa ggcctggggg aaattgccga gtgcctcgcc gctcgccccg
     5161 gtctcctcgg cgtagagccc gaggtggttg gcgtagccca gcatctgctc gaacaccagt
     5221 cgagcgcggt cgaggcgccc ccggtcgtgc cgcccggccc gcgtcatcgc ctccaccagc
     5281 cagaacgtgc agatgttgaa ggtcccttcc tcgccctcca gaccgtcggc ggcctcgcgc
     5341 acgttgtagc gatagacgag gctgttggag acgagcccgc cgcgctcggg cggctcgagc
     5401 gtggcgtcca gcgtcccgag catgcggccg tcggtcggcg agaggaagaa caccagcggc
     5461 atcatgaggt tggcggcgtc gaggcattcg ctgccgtagt gctgcacgaa ggccttccgg
     5521 cgcgaattcc accccgaggc catgatctcc tcgtacatca ggtcccggac cttcgaccac
     5581 cgctcgcggt cggcagggaa cgagcgcttg tcggccaggc gcagcgcccg atcgacggct
     5641 acccagcaca tgagcttgga gtagacgaag tgctgctgcc cgccgcggac ctcccagatg
     5701 ccctcgtccg tccgctgcca gttgtcgcag acccagttgg tgaggcggcg caggtgcacc
     5761 cagaagtcgt aggaaatcgg gttgccgtac ttgttgtaca ggtagacggc gtccatcagc
     5821 tcgccgtaga tgtcgagctg gagctggccg taggcgccgt tgccgatgcg gacgggccgc
     5881 gagccgcggt agccctcgag gtggtcgagc gtttcctcgg tcagcgtgtg ccgcccgtcg
     5941 atcccgtaca tgatctgcag cgagccgtcc gggttcagct cgtggcagcg cgcctcgatc
     6001 cactccatga agcgcgccgc ctcgtcggtg aagcccaggc gcagcagcgc gtacagcgtg
     6061 aacgcggcgt cgcggaccca cgtgtaccgg tagtcccagt tccgggggcc gccgagcccc
     6121 tcgggtaggc tgcacgtcgg ggccgcgacg atggcgccgg tcggctcgta cgtgagcagc
     6181 ttgagcgcca gcgccgagcg gtgcaccatc tcgcgccacc gccccgtgta gctgcaccgg
     6241 gccagccaac gcctccagaa gtccacggtg gcctcgaaca gcgcgtccga ctcggggtcg
     6301 gacaggccca ggccggcgtt ggcgccggcc gggatctgcc ggaggacgaa cgtctcgctc
     6361 tcgttctcgc tgagcacgaa gtcggcggtc actcctcgcg cgtcgcgcga cagcggaatt
     6421 ttcgtggcca ggccgaggct gaggctcggc gagcggaaga cggcgccctc cgaggtcagc
     6481 tccgtctcgt ggggatcgcg cgcgtagttg aaggcgggat ggcactcgag cctgacggga
     6541 atgctgccgc ggacggcggt gacccggcgt acgacctggt ggtagccgtc gcggtccggg
     6601 cggcggccca cgggcatgta gtccgtgatc tccgccacgc cgctcggcga gaggaagcgc
     6661 gagatgagga cgttggtatc cggccagtag agctgcttca ccttgacgtc gccgtaagcc
     6721 ggcgtgatcc ggaagcggcc gcccttcgcg tcgtcgagga tggccgcgaa gacgctgggg
     6781 gagtcgaacc ggggcaaaca gagccagtcg atcgatccgt tcatgccgac gagagccacc
     6841 gtgtggaggt cgccgatgat gccgtagttc tcgatcggct gatagggcat acgcgtcagt
     6901 gggtcgacga gcagggtaac acgtgcgtcc ggccgacccg acagaacctc gggatcgttc
     6961 cgaccggcgg gatcatctga gcacgggcat cacctgctgc gtgaggatct cgaggctccg
     7021 catctcccga gccatcggca gcagcccgcc cgggttcagc tcggcggcga tcccgttcac
     7081 gccggccgag ttcttcctgg agccgcccca ggcgatcgat cagtccgggg ccggtgccga
     7141 acgccacctt cttgctcagg atgtcctcgt acgagaggcc ggacagccgc tcggcctggc
     7201 gctcgcgccg ctcggcaggt cccgtccccg cccgaccgat cggcgcccgg gtgagatcgg
     7261 cctggcgctg gaagtagtag gtgatggtct cgtgcggctc ttcccgggcg gccgtctccg
     7321 tcggggcggc atagatcggt atccggaggc atgcgtcacc ggctccgggg tgccccgcgt
     7381 cgtgccacgc ctggcggtac atacgcaggc agcccgccag ctccggaatg tccatgcccc
     7441 gcagcccgac gaagatgggc aagcccatct tccccacgcg ggggaacgtc tccgtcgtcg
     7501 tcgccgccat ccggaccggc gggtgcggat ggcggtacgg gcgaggggtg acggtgacgt
     7561 tggagaaccg gtagaaccgt ccctcgtagc tgaagggctc gcccttccag gcctggagga
     7621 tgatctccag cgcctcgaag aagcgcgcct gactctcctc gtaggggacg gcgagagcgt
     7681 cgtaggcgcg cgggcctccg ctacgaccga cgccgaaatc gaaccgcccc tggctcagat
     7741 gatcgagcgt ggcgacctcg ccagccaccc gcaccgggtg gttgagcggc aggagctgga
     7801 cggcggttcc cacgcgcagg cgcctggtcc gggccgcgat ccagctcgcc accagaagtg
     7861 gcgccgacag caccgagcgg gtcgggttga ccatcatttc cgccagccag aagatgtcga
     7921 gtccccacgt ttccgcggca tccgcgagct cgaacatctc gcggaacgcg tcggcctggc
     7981 tcgtcccacg gcgcagctgc tcgacgaaca cgccgaattc catcgggtcc tctctcacgt
     8041 ccagtgatgc cgcgcgacgc cgtcggtgcg gcctcgcgtc ccgaaacggt ccggcggctc
     8101 acggtcgaga cggcggcggg atgctaccgc gggtggcgag cggaaacaat acgtgtcgtc
     8161 cacgcctccg gcgaccgcgg aggagcggcg gcccggacac cggatgcgcc ggcgcggccg
     8221 gcgccaccat ctggatcgtc ccgtggcgcc gatgtcccgg cttactcgta gcgcagcgcc
     8281 tcgatgggat cgaggcgcgc ggccttgcga gcgggataga agccgaagaa aacgccgatg
     8341 gccgcggaaa agccgaaggc cacgacgatc gtctccactg cgaccagcgt ccgccagtcg
     8401 gcgaagtagc tgatcgctcg ggacccaccg aggcccacgg ccacgccgac ggcgccgccg
     8461 atcagcgaca gggtgacggc ctcgaccagg aactgggtga ggatgtcccg tccccgcgcg
     8521 ccgaccgcca tccggaggcc gatctcacgg gtccgctcgg tcaccgacac cagcatgatg
     8581 ttcatgatcc cgatgccgcc cacgagcagc gagacggagg cgatggaggc gaggagagac
     8641 gccatcaccc gcgacgactc ctcctgggtc tgcagcacc
//
LOCUS       DATIAZ010000173        11201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_701406, whole genome shotgun sequence.
ACCESSION   DATIAZ010000173 DATIAZ010000000
VERSION     DATIAZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701406"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2209)
                     /locus_tag="VLF19_06175"
     CDS             complement(<1..2209)
                     /locus_tag="VLF19_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HSE92873.1"
                     /translation="MSWPRAPLVPPALAFAAGIALAPVLAPQTAWPLLIGALAWGTSL
                     VILERAAPATAFLLAGIVATGALRAAPLPPAPDDVVHLVLPRGAHVEGRLAAQPVRFA
                     PDRARLLLEVERVDAETRSGRVPLTAYGAGLPDLTGGQRVAVRARLHRAVGFRNPGGF
                     DYGAWLARDGILVTGSMRAEEVIPLDAPAPSWPARVRRAARETMMGSLPPVSAALLGG
                     LLFGDRVDLPGEIDVAFRRAGVYHVLAVSGFNVALVAGSVFALLTLTRAGRRGATFGA
                     IGAVLAFAAVVGGEPSVLRALVMGLAILGALLVDREASVVNGLALAALLILAVRPSDL
                     ADPGFQLSFAATAGIVLAPLPRHPLLAALGVSLAAQLAVLPIALVHFNQLTVVGPLAN
                     LAAVPLAGAATLVGLAAIALSAASGGAADVLLHAVWPLLLALRGVVALAAAVPGALLH
                     LPAPHWTAVTAYAAAAGLGLGAWHTRTTAPDRARWCGLAAGLLLAVAVGVALWPLARP
                     PDGRLRLTVLDVGQGDAIVVEAPDGRTLLVDAGPGGPMRLDAGERVVAPFLWNRGILR
                     LAAVITTHADADHAGGMPAIRRLFAVTRDWGPASIPRERQWIGGLVLWPLGDHVPAAT
                     RNDGAVVLRLDYGLASFILASDASGEAERHLIATRAPLAATVLKVGHHGARDASTPAF
                     LAAVRPALAAVSVGPRNAYGHPAPETLARLTAAGARILRTDHDGALLFDTDGRA"
     gene            complement(2213..2665)
                     /locus_tag="VLF19_06180"
     CDS             complement(2213..2665)
                     /locus_tag="VLF19_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSE92874.1"
                     /translation="MGLYTRHQIALLLALLAAAGFGLAVRHWRATHPDVATRLERFDL
                     GTQAEPQPRGDERAPAPGPLDLNRATTDDLARLPGVGPALAARIVAARQAGGFDSLDD
                     LTRVKGLGRTRIERLRPLLSVGDAPAAAAEPADVTGRPGKEAPDVTDE"
     gene            2812..3432
                     /locus_tag="VLF19_06185"
     CDS             2812..3432
                     /locus_tag="VLF19_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92875.1"
                     /translation="MIRFAALVFVLALLAAPIAMAQPSTPSLDQSGIVERIDQTGGVV
                     VLEGGRMYRITPSTVVSVNGQPVAFGSLRPGQTVIVRSAEPVMLQNGQYVVVTPGAGT
                     TVTTAPGAVTAAPATTVTPSGGGTVTTSPGSTVVVTAPPAAAARQTIYGQVSDVDPTE
                     IQIRTDKGKFEVRLPEAMRGQIKEGDNVQIDLTFTPTNPAASPRMR"
     gene            complement(3529..4674)
                     /locus_tag="VLF19_06190"
     CDS             complement(3529..4674)
                     /locus_tag="VLF19_06190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE92876.1"
                     /translation="MKFYYFHLMPYRMEHEEPSSWVTLSNRHYDPAVGHTLYNQYLDQ
                     FEHAERLGWDGLCVNEHHQNCYGTMPSPNLMAAMLARRTTRARIAVLGNGLPLRENPL
                     RIAEEIAMLDVVSGGRIVSGFVRGIGPEYYSVGANPTHSRERFYEAAELILRAWTEDG
                     PFPFDGRFYRYHWVNPWPRPLQKPHPPVWCPSQGSTETVEWAARRRFPYLMVFTPIAR
                     IAQVYGEYREACERFGYTASPYQLTVNLPIVVAENDAKAVRIAQAHGLWVYHTGLRMP
                     VPFWQPPGYLSEASFRRAMANRPKLPTELTFDDLEAGGYIIAGSPATVRDKLRIFSDE
                     LRAGIVCSGMPAGSHEATLDMMEMFAKEVMPHFRADAHVDETAGVGR"
     gene            complement(4671..5411)
                     /locus_tag="VLF19_06195"
     CDS             complement(4671..5411)
                     /locus_tag="VLF19_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE92877.1"
                     /translation="MLQAGGGTPLLFLHGAGAAGRWLTFHERLAGRFTVLAPSHPGHG
                     GSPAAEWIEHISDLAFHYLDLLDALGLDRVHLVGASFGGWIAAEMATMASHRLVSLTL
                     IAPVGIKVEGWIYPFLFAMELPQLVQTLFHDPMAAMALAPRDTATIDGLAEIYRERAA
                     LARVAWNPYLYDPLLRRRLARITAPTLLCWGEHDRLAPRVCADTWAREIPGATLATFD
                     RSGHAPHVEEPEAVAAAVIAFCAVREGR"
     gene            complement(5532..6755)
                     /locus_tag="VLF19_06200"
     CDS             complement(5532..6755)
                     /locus_tag="VLF19_06200"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSE92878.1"
                     /translation="MPLIVQKYGGSSVADAEKITSVARRVAEDARGNQVVVVVSAMGK
                     TTDGLVTLAARVTPTPDPREMDVLLATGEQVTIALLAMALQRLGYKARSFTGPQAGVR
                     TDTGHTKARITRITTERVRAALDAGEVAVVAGFQGLSEHDEITTLGRGGSDLTAVALA
                     AALEADVCEIYTDVDGVYTADPNIVPDARKLARITYDEMLELASLGAKVLQSRSVEFA
                     KKYGVPVHVRSTFKPDAGTLVTREERDMEEVVVTGVTHDRSQAKISILRVPDRPGIAA
                     QVFGAISTGNIVVDMIVQNISRDGYTDMSFTVARGEYKRAVAALQEVATKVGAQGLVH
                     DERVAKVSIVGVGMRSHSGVASRMFATLAREGINIQMISTSEIAVSCVIEDKYAELAV
                     RALHDAFEMGGVAKE"
     gene            complement(6865..7857)
                     /locus_tag="VLF19_06205"
     CDS             complement(6865..7857)
                     /locus_tag="VLF19_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSE92879.1"
                     /translation="MSRLKLAAGAVISVVLLGFLLWTVDLRQLAQSLRRTRWEWTLLS
                     VACGLAGLWIRARRWRYLFPPGSEPPALFPAVMIGYMVNNILPLRAGEVVRVYVVARR
                     WRRGFWTALATLIVERVLDSLAIVLILGVLVLLIPVPAIFRWTAATLLAVDAAAVVAL
                     AFLAAAPAAGRRLLTALTRRWPGLGERLTRVFDRFVLGLAGIRAPAHLLPLLAWSALV
                     WVAPALAAWTMLRALDLDLPLVVGWTVLAFVGMGISIPSAPGYVGVFHYAAVLALEVF
                     GVPRPAALGYAILFHATQFIPVTVVGWIYLLREQLTLGEATHARPADRAGTPLA"
     gene            complement(7868..8926)
                     /gene="thrC"
                     /locus_tag="VLF19_06210"
     CDS             complement(7868..8926)
                     /gene="thrC"
                     /locus_tag="VLF19_06210"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSE92880.1"
                     /translation="MGVWRGVIERYREFLPVTDKTPVVTLGEGNTPLVPAPRLAEASD
                     PTLSIYLKCEGFNPTGSFKDRGMTMAISKALESGSRAVICASTGNTSASAAAFAARAS
                     VKAFVMVPKGAIALGKLSQAAIHGARVLMVDGNFDQALSIVRRISETHPVTLVNSVNP
                     FRLEGQKTAAFEVVDQLGRAPDYHLIPVGNAGNITAYWRGYREYHRAGRATDLPRMVG
                     FQAAGAAPLYENRVIDEPRTVATAIKIGNPASWGPALEALKDSRGWVDIVTDEEILHA
                     YRMLAREEGIFMEPASAATVAGLLKNVKAGRFEPGSTLVLTLTGHGLKDPDTALESAS
                     RPTTVPANLTAVLAQLGL"
     gene            complement(8983..10290)
                     /locus_tag="VLF19_06215"
     CDS             complement(8983..10290)
                     /locus_tag="VLF19_06215"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSE92881.1"
                     /translation="MKDVRIGLLGLGTVGAGVAKLLESQRTLLEERAGCRLTLAAVAD
                     LDVTRPREGLDLARLPLTTDAGRVLGDPSIDVVVELCGGLEPARSFILKALGSGKHVV
                     TANKALLAHHGAELYEEARRRRVALGLEAAVAGGIPLIRAVKDGLVANRILSIFGIVN
                     GTCNYILSKMSEEGLDFSMVLKEAQAHGYAEADPTLDIEGLDSAHKLQILVTLAFRTF
                     VDLKHIHTEGITGITPQDIAYAGELGFRVKLLAIAKAADGGVEVRVHPTMIPAAAPLA
                     AVSGVFNAVFLTGDAVGDLMFYGRGAGQMPTASAVWSDIVEIARRIAYGIPSLPLELP
                     SPGPGALPLRTMDSIRSAYYLRVMAQDRPGVLSRVAGILGENNISIASVIQKERAAKE
                     AVPVVMMTHEAQERDMRAALGAIDRLRVVAAPTTMIRVEGGPK"
     gene            complement(10293..>11201)
                     /locus_tag="VLF19_06220"
     CDS             complement(10293..>11201)
                     /locus_tag="VLF19_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSE92882.1"
                     /translation="LDPDTETIATIGAKEGMAHLALAVLQPGDGVLVPNPTYPIHSYS
                     VVIADGDLRSVPLAGAADDFFARLQETARLAWPKAKMLILSFPHNPTTMCVDRAFFER
                     VVVFAREHHLMVVHDFAYADFAFDGYKPPSFLEVPGAREVGVEIFSTSKSYNMPGWRL
                     GFVCGNARMIHALARIKSYLDYGAFQPIQIAGIIALEGDQKCVDEIVEVHRRRRDVLV
                     DGLNKLGWTVAKPKGTMFVWAPIPDAFRPLGSLEFSKLLIQEAKVAVSPGIGFGEYGE
                     GYVRFALVENEQRIRQALRGLKHLGR"
BASE COUNT         1688 a         3938 c         4131 g         1444 t
ORIGIN      
        1 gcgcgcgccc gtccgtgtcg aagagcaggg ccccgtcgtg gtcggttctc aggattcgcg
       61 cgccggccgc ggtcagccgc gccagcgtct ccggggcggg gtgcccgtac gcgttgcgcg
      121 gaccgacgga caccgcggcc agcgccggcc gcaccgcggc caggaaggcc ggcgtgctcg
      181 cgtcccgggc gccgtggtgt cccaccttca ggacggtggc ggcgaggggc gcgcgggtgg
      241 cgatcaggtg ccgctcggcc tcgccgctcg catcggaggc gaggatgaac gacgcgagcc
      301 cgtagtccag acgcagcacg accgcgccgt cgttccgagt cgcggcaggg acatggtcgc
      361 cgagcggcca caggacgagc ccaccgatcc actggcgctc gcgcggaatc gatgccgggc
      421 cccagtcgcg cgtcacggcg aagaggcggc ggatcgccgg catgccgccc gcgtggtcgg
      481 cgtcggcgtg ggtggtgatc accgcggcga gcctgagaat gccgcgattc cagaggaacg
      541 gcgcgacgac ccgctctccc gcgtcgagtc gcatcggtcc gccggggccc gcgtccacca
      601 ggagcgtgcg gccgtccggc gcttcgacga cgatggcgtc gccctgaccg acgtcgagga
      661 cggtgagacg caggcgcccg tcgggcggtc gcgccagtgg ccacagggcc acccccaccg
      721 cgacggcgag caacaggcca gcggcgagac cgcaccatcg cgcgcggtcc ggcgccgtcg
      781 tccgcgtatg ccaggcgccg agtccgagcc cggccgccgc cgcgtacgcg gtgacggccg
      841 tccagtgggg cgcgggcaag tgaaggagcg cgcccggcac ggccgcggcg agggcgacga
      901 cgccgcgcag ggcgagcagg agcggccaga ccgcgtggag cagcacgtcc gccgcgccac
      961 cgctggcggc ggacagcgcg atggccgcga ggccgacgag ggtcgcggcg ccggcgagtg
     1021 gcaccgcggc caggttcgcc agcggtccca ccaccgtgag ctggttgaag tggacgaggg
     1081 cgatggggag caccgcgagc tgggccgcga ggctgacgcc gagcgcggcg agcaggggat
     1141 gccgcggcag cggcgccagc acgatgcctg ccgtggcggc gaacgagagc tggaagccgg
     1201 gatcggccag gtcgctcggc cgcacggcga ggatgagcag cgcggccagg gcgagcccgt
     1261 tgacgacgga cgcttcgcgg tcgacaagga gggcgccgag aatcgccagg cccatgacga
     1321 gggcgcgaag caccgagggc tcgccgccca cgaccgcggc gaaggcgagc acggcgccga
     1381 tcgccccgaa ggtggcgccg cgccggccgg cgcgagtcag cgtgaggagc gcgaacacgg
     1441 agccggcgac cagcgcgacg ttgaagccgg acaccgcgag cacgtggtag acgccggcgc
     1501 gacggaacgc cacgtcgatc tcgcccggca ggtcgacgcg gtcaccgaag agcagccccc
     1561 cgagcagcgc ggccgagacc ggcggcaggg agcccatcat cgtctcgcgc gccgcccggc
     1621 ggaccctggc gggccacgac ggcgcgggcg cgtcgagcgg aatcacctcc tcggcgcgca
     1681 tgcttccggt gacgaggatg ccgtcgcgcg cgagccaggc gccgtagtcg aagccaccgg
     1741 ggttgcggaa gccgacggca cggtgcaggc gagcgcgcac cgcgacgcgc tggcccccgg
     1801 tgagatcagg caggccggcg ccgtacgcag tgaggggcac gcgcccgctc cgcgtctcgg
     1861 cgtccacgcg ctcgacctcg agcagcaggc gcgctcgatc cggcgcgaag cgcacgggct
     1921 gggcggccag ccggccctcg acgtgggcgc cgcgcggcaa caccaggtga accacgtcgt
     1981 cgggcgccgg cggcagcggc gcggcccgga gtgcgcctgt agccacgatg ccggcgagca
     2041 ggaacgcggt ggcgggcgcc gcgcgctcca ggatcacgag gctcgtgccc cacgccagcg
     2101 cgccgatcag caggggccag gccgtctgcg gcgccagcac cggagcgagc gcgatgccgg
     2161 cggcgaaggc gagtgcaggt gggaccaggg gcgcgcgcgg ccacgacatc cctcattcgt
     2221 ccgtgacgtc gggcgcctct tttccgggcc gcccggtgac gtccgccggc tcggcggcgg
     2281 cggccggcgc gtcgcccacg ctcaggagcg ggcgcaaccg ctcgatgcgc gtgcgcccga
     2341 ggcccttcac gcgcgtgagg tcgtcgagcg agtcgaagcc gcccgcctgt cgcgcggcga
     2401 cgatccgcgc ggcgagcgcc gggcccacac caggaaggcg cgccaggtcg tcggtcgtcg
     2461 cgcggttgag atcgagcggc ccgggggccg gcgcccgctc gtcgccgcgg ggttgcggct
     2521 cggcctgcgt cccgaggtcg aagcgctcga gccgcgtcgc cacgtccgga tgcgtcgcac
     2581 gccagtggcg gaccgcgagc ccgaacccgg cggcggccag cagcgccagg agcagggcga
     2641 tttgatggcg ggtgtagagg cccacgtccc gacggtgcca ccgcgagcgt gacgccgcca
     2701 gagtcgaaaa attacgacac cttcagatcc acgacgatac gaccgccggc ccgtcgctgg
     2761 cagcgctctt gctcatctat cccgcgagcg atatctccag ggaggaaaac catgatcaga
     2821 tttgcagcgc tcgtgttcgt gctcgcgctg ctcgccgcgc cgatcgccat ggctcaaccg
     2881 tcgaccccgt cgctcgacca gtccggcatc gtcgagcgga tcgaccagac agggggagtc
     2941 gtcgtgctcg agggcgggcg gatgtaccgc atcacgccca gcaccgtggt gtccgtgaac
     3001 ggccagcccg tcgcgttcgg ctcgctgcgg ccgggccaga ccgtcatcgt acgctccgcc
     3061 gagcccgtca tgctccagaa cggccagtac gtcgtcgtga cccccggtgc cggcaccacg
     3121 gtgaccacgg cgccaggggc cgtgaccgcc gcccccgcca ccacggtgac accctcgggc
     3181 ggggggacgg tgacgacctc gcccggcagc acggtcgtcg tcacggcacc gcccgccgcg
     3241 gccgccaggc agacgatcta cggacaggtc agcgacgtgg accccaccga gatccagatc
     3301 aggacggaca agggcaagtt cgaggtcagg ctgcccgagg ccatgcgtgg ccagatcaag
     3361 gagggcgaca acgtccagat cgacctgacg ttcacgccca cgaaccccgc ggcgtccccc
     3421 cgcatgcgct agaccccgac gcgttcgagc gccggctgtg ggtgccggcg caatcgaatc
     3481 cgaaggggga ggattcggag ggggccgtcg aggccccctc cgaggctctc agcggcccac
     3541 gcccgcggtc tcgtccacgt gcgcgtccgc gcggaagtgg ggcatcactt ccttcgcgaa
     3601 catctccatc atgtcgagcg tcgcctcgtg gctgcccgcc ggcatgcccg agcacacgat
     3661 ccccgcgcgc agctcgtcgg agaagatgcg cagcttgtcg cgcaccgtcg ccggggagcc
     3721 cgcgatgatg tagcccccgg cctcgagatc gtcgaacgtc agctccgtgg ggagcttcgg
     3781 ccgattggcc atcgcgcgtc ggaacgacgc ctcgctcaga tagcccggcg gctgccagaa
     3841 agggaccggc atgcgcagcc ccgtgtggta cacccacagg ccgtgcgcct gggcgatcct
     3901 caccgccttg gcgtcgttct cggcgacgac gatcgggagg ttcacggtga gctgataggg
     3961 gctcgccgtg tagccgaagc gctcgcaggc ctcgcggtat tcgccgtaga cctgcgcgat
     4021 gcgcgcgatc ggcgtgaaga ccatcaggta agggaagcgc cgcctcgcgg cccactcgac
     4081 ggtctccgtg ctgccctggg acggacacca cacgggcgga tggggcttct gcagaggccg
     4141 cggccacggg ttgacccagt gataccgata gaagcggccg tcgaagggga aggggccgtc
     4201 ctccgtccac gctctcaaga tcagctccgc cgcctcgtag aaccgctcgc gcgagtgcgt
     4261 cggattcgcg cccaccgagt agtactcggg gccgatgccg cgcacgaagc ccgagacgat
     4321 ccgcccgccc gagacgacgt cgagcatggc gatctcctcg gcgatgcgca gggggttctc
     4381 gcggagcggc agcccgttgc cgagcacggc gatgcgcgcg cgcgtggtgc gccgcgccag
     4441 catggccgcc atgaggttgg ggctcggcat ggtgccgtag cagttctggt ggtgctcgtt
     4501 cacgcagagg ccgtcccagc ccaggcgctc ggcgtgctcg aactggtcga ggtactggtt
     4561 gtagagcgtg tggccgaccg cggggtcgta gtggcggttg gagagcgtca cccacgagga
     4621 cggctcctcg tgctccatcc ggtacggcat caggtggaag tagtagaact tcatcggccc
     4681 tcccggaccg cgcagaacgc gatgaccgcc gccgcgaccg cctccggttc ctcgacgtgc
     4741 ggcgcgtggc ccgagcggtc gaacgtcgca agcgtcgcgc ccgggatctc cctggcccac
     4801 gtgtcggcgc agacgcgggg cgccaggcgg tcgtgctcgc cccagcagag cagcgtcggc
     4861 gccgtgatgc gcgcgagccg gcgccgcagc agcggatcgt acaggtacgg gttccacgcc
     4921 acgcgcgcca gcgccgcgcg ctcgcggtag atctcggcga ggccgtcgat cgtggccgtg
     4981 tcgcggggcg ccagggccat cgccgccatg gggtcgtgga agagcgtctg cacgagctgc
     5041 ggcagctcca tggcgaagag gaacgggtag atccagccct cgaccttgat gcccaccggc
     5101 gcgatgagcg tgagcgagac gagccggtgc gacgccatcg tggccatctc ggcggcgatc
     5161 cagccgccga aggacgcgcc gacgagatgg acgcggtcga gacccagcgc gtcgagcagg
     5221 tcgaggtagt ggaaggcgag atccgagatg tgctcgatcc actccgcggc cggcgagccg
     5281 ccgtggccgg ggtggctggg cgcgagcacg gtgaagcgtc cggccagccg ctcgtgaaag
     5341 gtcagccacc gtcccgcggc gccggcgccg tggaggaaca gcagcggggt cccgccacct
     5401 gcctgcagca tatggatcgg ggtcccgcgc acgacgaggc ggcggtcggc cggcgtcgct
     5461 ccgtcactcg cggcgccgga cgacgccaca ccgtcagcgg tgtgctcgag atccgcctgg
     5521 cgccccagac ctcactcctt ggccacgccc cccatctcga aggcgtcgtg cagggcgcgg
     5581 acggccagct ccgcgtactt gtcctcgatg acgcaggaga cggcgatctc ggaggtcgag
     5641 atcatctgga tgttgatgcc ctcgcgcgcc agcgtggcga acatgcgcga ggccacgccg
     5701 gagtggctcc gcatgccgac gccgacgatg gagaccttcg ccacccgctc gtcgtggacg
     5761 aggccctggg ctccgacctt cgtggccacc tcctggaggg ccgccaccgc gcgcttgtac
     5821 tcgccgcggg caaccgtgaa cgacatgtcg gtgtagccgt cgcggctgat gttctgcacg
     5881 atcatgtcca ccacgatgtt gcccgtggag atggcgccga acacctgggc ggcgatcccc
     5941 ggccggtccg gcacccgcag gatggagatc ttcgcctggc tccggtcgtg ggtgacgccg
     6001 gtgacgacca cctcttccat gtcccgctcc tcccgcgtca cgagggtgcc ggcgtcgggc
     6061 ttgaaggtcg accgcacgtg caccggcacg ccgtacttct tggcgaactc caccgagcgg
     6121 ctctggagga ccttcgcgcc cagactggcc agctcgagca tctcgtcgta ggtgatgcgg
     6181 gccagcttgc gcgcatcggg gacgatgttc gggtcggccg tgtagacgcc gtccacgtcg
     6241 gtgtagatct cgcagacgtc cgcctcgagc gcggcggcga gcgccaccgc ggtgaggtcg
     6301 gagccgccgc ggccgagcgt ggtgatctcg tcgtgctccg agagtccctg gaagccggcg
     6361 accacggcga cctcgccggc gtcgagggcc gcgcgcaccc gctcggtcgt gatgcgggtg
     6421 atgcgcgcct tggtgtggcc ggtgtccgtc cgcacgccgg cctgcggccc cgtgaaggag
     6481 cgcgccttgt acccgagccg ctgcagggcc atcgcgagca gcgcgatcgt cacctgctcg
     6541 ccggtggcga gcagcacgtc catctcgcgc gggtcggggg tcggcgtgac ccgggcggcg
     6601 agggtgacga ggccgtcggt ggtcttgccc atggccgaga ccacgacgac gacctgattg
     6661 ccgcgcgcgt cctcggccac gcggcgggcg acgctggtga tcttctccgc gtcggcgacg
     6721 gaggagccgc cgtacttctg cacgatcagc ggcatggctc cgggcatcgt atcacgggag
     6781 ccggcgccgg cccggcggac gccggcgccc tgttgcgcca ggcctccgac cgaccggaga
     6841 cgctgccggt gagcgggtct ggcttcacgc gagtggggta cccgcccggt cggcggggcg
     6901 cgcgtgggtg gcctcgccca gcgtgagctg ctcccgcagg aggtaaatcc acccgacgac
     6961 cgtcacgggg atgaactgtg tcgcgtggaa gaggatcgcg tagcccagcg cggcgggccg
     7021 gggcaccccg aacacctcga gcgccagcac cgccgcgtag tggaacacgc cgacataccc
     7081 gggcgccgag gggatgctga tgcccatccc gacgaacgcc agcaccgtcc atcccacgac
     7141 cagcggcagg tcgaggtcca gcgcccggag catcgtccac gcggccagcg ccggcgcgac
     7201 ccagacgagg gccgaccagg cgagcagggg cagcaagtgg gccggcgcgc ggatccccgc
     7261 gagcccgagc acgaagcggt cgaagacgcg cgtgagacgc tcgccgagtc ccggccaccg
     7321 acgggtcagc gcggtcaaca gtctccggcc ggccgccggc gcggcggcca ggaacgcgag
     7381 cgccacgacc gccgccgcgt ccacggcgag cagcgtcgcg gccgtccagc ggaagatcgc
     7441 aggcaccggg atgagcagga cgagcacgcc gaggatcagg acgatggcca ggctgtccag
     7501 cacccgctcg acgatcagcg tcgccagcgc cgtccagaag ccgcgccgcc agcggcgcgc
     7561 gacgacgtag accctgacga cctcccccgc gcggaggggc aggatgttgt tgaccatgta
     7621 gccgatcatg acggccggga agagggcggg gggctccgag ccgggcggaa agaggtagcg
     7681 ccagcggcga gcgcggatcc agaggccagc caggccgcac gccaccgaga ggagcgtcca
     7741 ctcccaccgc gtgcgcctga gcgactgggc gagctggcgg aggtcgaccg tccacagcag
     7801 gaagccgagg aggacgacgc tgatgacggc gccggccgcg agcttgaggc gactcacccg
     7861 gtccgggtca gagcccgagc tgcgcgagga ccgccgtgag gttggcgggc accgtggtcg
     7921 ggcgcgaggc ggactcgagc gcggtgtccg ggtctttgag gccgtgcccg gtgagtgtca
     7981 acacgagcgt cgagccgggc tcgaagcgcc cggccttgac gttcttcagc aggccggcga
     8041 ccgtcgccgc ggaggcgggc tccatgaaga tgccctcctc ccgcgcgagc atccggtacg
     8101 cgtgcaagat ctcctcgtcg gtcacgatgt ccacccagcc ccgcgagtcc ttcagcgcct
     8161 cgagcgcggg cccccacgag gccgggttgc cgatcttgat cgcggtcgcc accgttcgcg
     8221 gctcgtcgat cacgcggttc tcgtacaggg gcgccgcgcc cgccgcctgg aagccaacca
     8281 tgcggggcag atcggtggcg cgtccggcgc ggtggtactc gcggtagcca cgccagtagg
     8341 cggtgatgtt gccggcgttg cccacgggga tcaggtggta gtccggcgcg cggccgagct
     8401 gatcgaccac ctcgaacgcc gccgtcttct gtccctcgag gcggaacggg ttcaccgagt
     8461 tgacgagcgt gaccgggtgg gtctcggaga tccggcgcac gatcgagagc gcctggtcga
     8521 agtttccgtc gaccatcagc accctggcgc cgtgaatggc cgcctgcgac agcttgccga
     8581 gcgcgatcgc ccccttgggc accatgacga acgccttgac ggaggcgcgg gcggcgaagg
     8641 cggcggccga ggccgacgtg ttgcccgtcg aggcgcagat gacggcgcgg gagccggact
     8701 cgagcgcctt ggagatggcc atggtcatcc cgcggtcctt gaacgagccg gtcgggttga
     8761 acccctcaca cttgagatag atgctgaggg tgggatccga ggcctcggcc agccgcggcg
     8821 ccggcaccag cggtgtattc ccctcgccca acgtcaccac cggcgtcttg tccgtcaccg
     8881 gcagaaactc tcgataccgc tcgatcaccc cccgccacac gcccatggtc cgctagcgcc
     8941 ctttcgaccg ccggctttgc cggccgtcga ggccccggcg attcatttcg ggccgccctc
     9001 gacgcggatc atcgtggtcg gcgcggccac gacgcgcagc cgatcgatcg cgccgagggc
     9061 ggcgcgcatg tcgcgctcct gcgcctcgtg cgtcatcatg accacgggga cggcttcctt
     9121 cgccgcgcgc tctttctgga tgaccgaggc gatcgagatg ttgttctcgc cgaggatccc
     9181 cgcgacgcgc gacaggacgc ccggacggtc ctgggccatc acgcgcaggt agtacgccga
     9241 gcggatcgag tccatggtgc gaagcgggag cgctcccggc cccggggacg gcagctcgag
     9301 aggcagggac gggatgccgt aggcgatccg gcgggcgatc tcgacgatgt ccgaccacac
     9361 ggcggaggcc gtgggcatct gtcccgcccc ccgaccgtag aacatcaggt caccgacggc
     9421 gtcgccggtc aggaacacgg cgttgaagac accggacacg gcggccagcg gcgccgccgc
     9481 cgggatcatg gtcgggtgca cacgcacctc gacgccgccg tcggcggcct tggcgatggc
     9541 cagcagcttc acccggaagc ccagctcgcc ggcgtaggcg atgtcctgag gcgtgatgcc
     9601 ggtgatgccc tccgtgtgga tgtgcttgag gtccacgaag gtccggaagg cgagcgtgac
     9661 gaggatctgc agcttgtggg cggagtccag gccctcgatg tcgagcgtgg ggtcggcctc
     9721 cgcgtagccg tgagcctgcg cctccttcag caccatcgag aagtccaggc cttcctcgct
     9781 catcttcgag aggatgtagt tgcacgtccc gttgacgatg ccgaagatgg agaggatccg
     9841 gttggcgacc aggccgtcct tgacggcgcg gatcagcggg atgccgccgg ccacggcggc
     9901 ttcgaggccg agcgccaccc ggcgccgccg cgcctcctcg tacagctcgg ccccgtggtg
     9961 ggcgagcagg gccttgttcg ccgtgaccac gtgcttgccg cttccgagcg ccttgaggat
    10021 gaacgaccgc gcgggctcca ggcccccgca gagctcgacg acgacgtcga tggacggatc
    10081 cccgagcacg cggccggcat cggtcgtgag cggcaggcgg gcgaggtcga ggccctcgcg
    10141 gggacgcgtc acgtcgaggt cggccacggc ggccagcgtg agccggcagc ccgcccgctc
    10201 ctccagcagc gtgcgctgcg actcgagaag cttcgcgacg ccagccccga cggtgccgag
    10261 accgagcagg ccgatgcgga cgtccttcac ggtcagcggc cgaggtgctt gaggccccgg
    10321 agcgcctgcc ggatgcgctg ctcgttctcc acgagcgcga agcggacgta cccctcgccg
    10381 tactcgccga agccgatgcc gggtgacacc gccacctttg cctcctggat gaggagcttg
    10441 gagaactcga gcgatccgag cgggcggaag gcgtcgggga tgggcgccca cacgaacatc
    10501 gttcccttcg gcttggccac cgtccacccg agcttgttca ggccgtccac gagcacgtcc
    10561 cggcgccggc gatggacctc gacgatctcg tccacgcact tctggtcgcc ctcgagcgcg
    10621 atgatgccgg cgatctggat cggctggaaa gcgccgtaat cgaggtagga cttgatgcgg
    10681 gccagcgcgt ggatcatgcg cgcgttaccg cacacgaagc cgagccgcca gccgggcatg
    10741 ttgtacgact tcgacgtcga gaagatctcg accccgacct cccgcgcgcc ggggacctcg
    10801 aggaacgacg gcggcttgta gccgtcgaag gcgaagtcgg cgtaggcgaa gtcgtggacg
    10861 accatgaggt ggtgctcacg ggcgaacacg accacgcgct cgaagaaggc gcggtccacg
    10921 cacatcgtcg tcggattgtg cgggaacgac aggatcagca tcttggcctt gggccacgcc
    10981 aggcgcgccg tctcctggag ccgtgcgaag aagtcgtcgg ccgcgccggc gagcggcacc
    11041 gagcgcaagt cgccgtcggc gatcacgacc gagtacgagt ggatcgggta ggtcgggttc
    11101 gggaccagca cgccgtcgcc cggctggagc accgcgagag cgaggtgggc catgccctcc
    11161 ttggccccga tcgtggcgat cgtctccgta tcgggatcga g
//
LOCUS       DATIAZ010000174         4559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_571605, whole genome shotgun sequence.
ACCESSION   DATIAZ010000174 DATIAZ010000000
VERSION     DATIAZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571605"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /gene="cofG"
                     /locus_tag="VLF19_06225"
     CDS             <1..815
                     /gene="cofG"
                     /locus_tag="VLF19_06225"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HSE92883.1"
                     /translation="KPRIPMRDHPEPPLDDLLRTLAESPLLPHANPGVMGERDLAALR
                     EVTVSMGIMLETVSERLLGAGMAHEVAPDKVPARRLRTIGLAGKLGIPFTTGLLIGIG
                     ETPRERVDALVAIRDLHERHGHVQEVIVQSFRTKPRIPMRDHPEPPLDDLLRTLAVAR
                     LLLGPDVNLQAPPNLSPGVYPRLLAAGLNDWGGISPLTVDHINPEAPWPLIPELRRAT
                     ESEGFTLRERLAVYPEFATRPEFVAEPLRPRVAALIGDDGLVRESHEHWRAW"
     gene            793..2058
                     /gene="cofH"
                     /locus_tag="VLF19_06230"
     CDS             793..2058
                     /gene="cofH"
                     /locus_tag="VLF19_06230"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HSE92884.1"
                     /translation="MSTGEPGSLRPALDWAAAAVRSALARALDGGELSIGDGLALATA
                     RGRDLHAVTLAADEMRRRQAGEHVTFVINRNINFTNVCIKHCTFCAFSRDHREEEGYL
                     LPVEEVVRRAREAVDLGATEVCIQAGLPPKLDGRFYIDLCRALKAALPDLHLHAFSPE
                     EILYGTVRSGMAIKDYLVELKGAGLGTLPGTSAEILDQDIRDRIARGRITVAQWVEIV
                     TTAHALGIRTTSTIMYGHVEAPTHWVRHMALLRDIQKDTGGFTEFVPLSLIHQEAPMW
                     SKHLVPGVRPGATGAEVVAMHALARLILGPVIPNVQASWVKEGPKLAQLLLAAGANDL
                     GGTLINESISTSAGAGFGQLVPPAELKRLVRDAGRVPAQRDTLYRLLKVYDADDDEAS
                     PLDAIDDADARFGSYRRLIASGQFRYTRG"
     gene            2137..2415
                     /locus_tag="VLF19_06235"
     CDS             2137..2415
                     /locus_tag="VLF19_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSE92885.1"
                     /translation="MMTKSATMSHLAQKTGLSKKQIVEVFDETLALACKEAKNGFVLP
                     GFGKLVLANRKARIGRNPQTGEPIKIPAKRVCKFRLTKSLKDTVLGKK"
     gene            2402..2995
                     /locus_tag="VLF19_06240"
     CDS             2402..2995
                     /locus_tag="VLF19_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92886.1"
                     /translation="MGRSSLGRRRSAGQRVPAAGAEAASTGVGLAPGPSLTGATTPRV
                     WQGTGYRPLPDPSVTRALFEFTAVPWPDPGRVALAWGAFAGGGGAAERLVGAVVGEGE
                     GRSTLLHGPVVVEHADPLEVAAQLVAAVLDHATALGADTIFARPLGLDRVWVRFGFIP
                     VPESTLPAALAGRSGAGLYAWRGGSALWTLREAPRET"
     gene            3006..3956
                     /gene="cofD"
                     /locus_tag="VLF19_06245"
     CDS             3006..3956
                     /gene="cofD"
                     /locus_tag="VLF19_06245"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HSE92887.1"
                     /translation="MKVAALAGGTGAAKLLRGLAAGLGRGQLTVVANTGDDADIWGLH
                     VSPDVDTVTYALAGRLDAERGWGLAGETFRCLEAMAGLGGETWFNLGDRDLATHLYRT
                     RRLRAGASLSVVTDEIAGRLGVRARILPMSDDAIRTRIRTPHAAGGEVWLGFQEYFVR
                     EKALVEVLEVAYEGAARARPAPGVLEALAHADLVVVCPSNPVTSIGPILAVPGVVEAL
                     AAVRATVIGVSPIVGGAPVSGPAGRLMHARGLPVSPVGVAAAYAPWLDALLIDPRDAS
                     CAPALRERGVAPVVTDVMMPDGPREVALAQVVLRHRRQET"
     gene            4024..>4559
                     /gene="cofC"
                     /locus_tag="VLF19_06250"
     CDS             4024..>4559
                     /gene="cofC"
                     /locus_tag="VLF19_06250"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HSE92888.1"
                     /translation="MIVAAVPVKDLVNAKQRLANVLTSLERRALARAMLADVLRALAI
                     TGVDRVWVVTRDQEVRAVAGEARAEVLVEDENRGHTAAVACAQAEAVRRGADVFLTVP
                     GDVPCVGSDEIDALARAAADRPTAVFAPSRSGRGTNGVALNPPDAMPLTFGEPSFDSH
                     LAAARERGLQPRVLRLPRL"
BASE COUNT          617 a         1727 c         1603 g          612 t
ORIGIN      
        1 ccaagccgcg catcccgatg cgcgaccacc ccgagccgcc gctcgacgac ctgctccgga
       61 cgctcgccga atcccccctg ctgccgcacg ccaaccccgg cgtgatgggc gagcgtgacc
      121 tggcggcgct acgcgaggtg acggtgagca tgggcatcat gctcgagacc gtctccgagc
      181 gtctcctggg ggccggcatg gcgcacgagg tggcgccgga caaggtgccc gcgcggcgcc
      241 tgcgcacgat cgggctggcc ggcaagctcg gcatcccctt taccaccggg ctcctcatcg
      301 gtatcggcga gacaccgcgc gagcgggtgg acgcgctcgt ggccatccgc gacctgcacg
      361 agcgccacgg tcacgtccag gaggtcatcg tccagagctt ccgcaccaag ccgcgcatcc
      421 cgatgcgcga ccaccccgag ccgccgctcg acgacctgct ccggacgctc gccgtcgcgc
      481 gcctgctgct gggccccgac gtcaacctcc aggcgccgcc gaacctctcg ccgggggtct
      541 acccgcgcct gctggcagcc ggcctcaacg actggggggg catctcgccg ctcaccgtcg
      601 accacatcaa ccccgaggcg ccgtggccgc tcattcccga gctccggcgc gcgaccgaga
      661 gcgagggctt caccctccgg gaacggctcg ccgtctatcc ggagttcgcc acgcggcccg
      721 agttcgtggc cgagccgctc cgtccgcgcg tcgccgcgct gatcggcgac gacggcctcg
      781 tgagggaatc ccatgagcac tggcgagcct ggtagcctcc gccccgcact ggactgggcg
      841 gccgccgcgg tgcgctccgc gctcgcgcgc gccctggacg gcggcgagct ctcgatcggc
      901 gacgggctgg cgctcgccac cgcgcggggc cgcgacctgc acgccgtcac gctggccgcc
      961 gacgagatgc gccgccgcca ggccggcgag cacgtgacgt tcgtgatcaa ccgcaacatc
     1021 aacttcacga acgtctgcat caagcactgc acgttctgcg cgttcagccg cgaccaccgc
     1081 gaggaggagg gctatctcct gcccgtcgag gaggtcgtcc gccgcgcgcg cgaggccgtg
     1141 gacctcggcg ccaccgaggt gtgcatccag gccgggctgc cgcccaagct cgacggacgc
     1201 ttctacatcg acctctgccg ggccctgaag gccgcgctgc cggacctcca cctgcacgcg
     1261 ttctcgccgg aggagatcct ctacggcacc gtgcggtcag gcatggcgat caaggactac
     1321 ctcgtcgagc tgaagggcgc gggcctcggc acgctgccgg gcacctctgc cgagatcctc
     1381 gaccaggaca tccgcgaccg catcgcgcgc ggccgcatca ccgtggcgca gtgggtggag
     1441 atcgtcacca ccgcgcacgc gctgggcatc cgcacgacct ccaccatcat gtacggccac
     1501 gtcgaggcgc cgacccactg ggtgcggcac atggcgctgc tgcgcgacat ccagaaggac
     1561 accggcggct tcacggagtt cgtgccgctc tcgctcatcc accaggaagc accgatgtgg
     1621 tccaagcacc tcgtgcccgg cgtgcgcccg ggcgcgaccg gcgccgaggt cgtcgccatg
     1681 cacgcgctcg cgcgcctcat cctcggcccc gtgatcccca acgtgcaggc ctcgtgggtg
     1741 aaggaggggc caaagctcgc gcagctgctc ctggccgcgg gcgcgaacga cctcgggggc
     1801 acgctcatca acgagtcgat ctcgacgtcg gccggcgccg gcttcgggca gctcgttccg
     1861 cccgcagagc tcaagcgtct cgtccgcgac gccggacggg tgcccgcgca gcgcgacacg
     1921 ctctaccgcc tgctcaaggt gtacgacgcc gacgacgacg aggcgagtcc gctggacgcg
     1981 atcgacgacg cggacgcgcg cttcggctcg taccggcggc tcatcgcgtc gggccagttc
     2041 cggtacacgc gcgggtagcc gcgtccagca gcctgtggta ttaattcacg tcgcgctcaa
     2101 ctcgaccaca caaccaggag gctacccgtg gcgaagatga tgacgaagtc ggcaacaatg
     2161 tcccacctcg ctcagaagac ggggctgtcg aagaaacaga tcgtcgaggt cttcgacgag
     2221 acgctggcgc tcgcctgcaa ggaagcgaag aacgggttcg tcctgccggg cttcggcaag
     2281 ctcgtcctcg ccaaccgcaa ggcgcggatc ggccgcaacc cgcagaccgg cgagccgatc
     2341 aagattccgg ccaagcgggt gtgcaagttc cgtctcacga agagtctcaa ggacaccgtg
     2401 cttgggaaga agtagtctcg gccggcgccg gtccgccggg cagagggttc cggcggccgg
     2461 ggccgaagcc gcgtccacgg gggtcgggct ggcgcctggc ccatcgctga ccggcgcgac
     2521 cacgccgcgc gtatggcagg gcacggggta ccgtcccctg cccgacccgt ccgtcacccg
     2581 ggcgctcttc gagttcaccg ccgtgccctg gcccgatcct ggccgcgtgg cgctcgcgtg
     2641 gggcgccttc gcgggcggcg gaggcgccgc cgagcgtctg gtcggcgcgg tcgtgggcga
     2701 gggcgagggc cgctcgacgc tgctgcacgg tcccgtcgtc gtggagcacg ccgacccgct
     2761 ggaggtcgca gcccagctcg tcgccgccgt tctcgaccac gcgacggcgc tcggcgcgga
     2821 cacgatcttc gctcgcccgc tggggctcga ccgtgtctgg gtccgcttcg gcttcatccc
     2881 cgtcccggag agcacgctgc cggcggcgct cgccgggcgt tcgggcgccg ggctctatgc
     2941 ctggcgcggc ggcagcgcgc tctggacgct gcgcgaagcc ccacgcgaga cgtgagacgc
     3001 cggagatgaa ggtcgcggcg ctcgccggcg gcaccggcgc cgccaagctg ctccgcggcc
     3061 tcgccgcggg cctcgggcgc ggccagctca cagtcgtcgc caacaccggc gacgacgcgg
     3121 acatctgggg tctccacgtc tcgccggacg tcgacaccgt cacctacgcg ctcgccggcc
     3181 ggctcgacgc cgagcgcggc tggggcctcg ccggcgagac gttccggtgc ctcgaggcga
     3241 tggcgggcct cgggggcgag acgtggttca acctcggcga tcgcgacctc gccacgcacc
     3301 tctatcgcac gcgacggctg cgcgcgggcg cgtcgctgtc cgtcgtcacc gacgagatcg
     3361 ccgggcgcct cggcgtgcgg gcccggatcc tgcccatgag cgacgacgcc atccgcacgc
     3421 gcatccggac gccgcacgcc gccggaggcg aggtctggct cggcttccag gaatatttcg
     3481 tgagggaaaa ggcgctcgtg gaggtgctgg aggtggccta cgagggcgcg gcacgcgccc
     3541 ggccggcgcc gggcgtgctc gaggcgctgg ctcacgccga tctcgtcgtc gtctgcccgt
     3601 cgaacccggt gacctccatc gggccgattc tcgccgtgcc gggggtcgtg gaggcgctcg
     3661 ccgccgtgcg cgccaccgtg atcggcgtga gcccgatcgt gggcggcgcg cccgtgagcg
     3721 ggcccgccgg acgtctcatg catgcccgcg gcctgcccgt gtcgcccgtc ggcgtggccg
     3781 cggcctacgc gccgtggctc gacgcgctgc tgatcgatcc tcgggacgcg tcgtgcgcgc
     3841 cggcgctgcg cgagcgaggc gtggcgcccg tcgtcaccga cgtgatgatg cccgacggcc
     3901 cgcgcgaggt ggcgctggcg caggtcgtgc tccggcaccg acggcaagag acatgaccgc
     3961 acgggatctc tggccgcccg tcgagcgccg gagccctgcg caggcgctcc gacggcaaat
     4021 cgaatgatcg tcgcggccgt cccggtgaag gacctcgtca acgccaagca gcggctcgcc
     4081 aacgtgctga cctcgctcga gcgacgcgcc ctcgctcggg ccatgctggc ggacgtgctg
     4141 cgcgcgctgg cgatcacagg tgtggatcgc gtgtgggtcg tcacccgcga ccaggaggtc
     4201 cgggcggtgg ccggcgaggc ccgtgccgaa gtgctggtgg aagacgaaaa ccgaggccac
     4261 accgcggcgg tggcgtgcgc gcaagccgag gccgtccggc gcggcgccga cgtgttcctc
     4321 accgtgcccg gcgacgtccc ctgcgtggga tccgacgaga tcgacgccct ggcacgcgcg
     4381 gccgccgacc gcccgacggc cgtcttcgcc ccctcgcgct cgggccgagg caccaacggc
     4441 gtggccctca accctcccga cgccatgccg ctcaccttcg gggagccctc gttcgacagc
     4501 cacctggccg ccgcccgcga gcgcggcctg cagccgcgcg tgctgcgcct gccgcggct
//
LOCUS       DATIAZ010000175        10381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_964830, whole genome shotgun sequence.
ACCESSION   DATIAZ010000175 DATIAZ010000000
VERSION     DATIAZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964830"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VLF19_06255"
     CDS             complement(<1..406)
                     /locus_tag="VLF19_06255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92889.1"
                     /translation="MSRPPRIHPAWILLAALALCMMAASGLRNVFGVYIKPMETEFGW
                     TRGALSVAAAASLLLLGAVGPLLGRLADRWGPRRVIIASLLVAGVGSISASAIQHLWH
                     VYVTIGLLLTAGAGGVAMTTGAALIARWFEARR"
     gene            complement(676..3282)
                     /gene="clpB"
                     /locus_tag="VLF19_06260"
     CDS             complement(676..3282)
                     /gene="clpB"
                     /locus_tag="VLF19_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HSE92890.1"
                     /translation="MAPRFDKYTVKAREAVQAAQAIAERQDHQAIEPEHLLLALLEQR
                     EGVVGPLLAKLGARPETLQATLEAELARMPKVRGAAGQYAGQRVLGVFERAQSEADRL
                     KDEYVSAEHLLVALADGDGAAARVLTANGVTKDAIYRALQDVRGTQRVTDENPEGKYQ
                     ALQRYSRDLTEAARKHKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVAGDVPEGLKNKRLVALDIGAMVAGSKYRGEFEDRLKAVLKEIIESNGEIIC
                     FIDELHTLVGAGAAEGAVDAANMLKPALARGELRCVGATTLDEYRKHVEKDPALERRF
                     QPVTVREPSVEDTISILRGLKERYEQHHKVKIKDAALVAAAVLSHRYIADRFLPDKAI
                     DLVDEAAARIRMQIDSKPQAIDEIERRVMQLEIDRVSVARDDDPASRERLGRIDAELS
                     ELKAKVEALNAQWQREKQGLNQATTLKAQLEGARQQLADAMRQGDYNRAAELQYGTIP
                     TLERQLAAADGTGEPPAAGDTARMLREEVDEEDIAATVAKWTGIPVTRLLEGETQKLL
                     SMEDRLHRRVVGQDEAIRAVANAVRRARSGLSDPNRPIGSFLFLGPTGVGKTELARAL
                     AEFLFDDERAMIRLDMSEYMEKHTVARLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLF
                     DEIEKAHHDVFNVLLQLLDDGRLTDGHGRTVDFRNTVVIMTSNLGSQLFRDYEQPEKV
                     RPLLMAELRNTLRPEFLNRIDEVVVFTPLGRAELARIVEIQLGHLRRRLADRRIELEV
                     TDAAKALLAREGYDPTFGARPLKRTIQRLVQDPLALKILEREFSEGDTVTVDAAGEGI
                     VFKRAVAAEVAG"
     gene            complement(3291..4346)
                     /locus_tag="VLF19_06265"
     CDS             complement(3291..4346)
                     /locus_tag="VLF19_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HSE92891.1"
                     /translation="MAVEFKDYYKILGVERSADAKALKSAYRKLARKYHPDVAKGKDT
                     AGRFAEISEAYEVLSDPEKRRRYDSLGPDWQRFAQPPPGGGFRVEHGEGGDFSDFFRT
                     IFGDFAARGAGDARTRGGAGQDAGFDLGDLLGGAFRRGAGRPRVARGEDVQAAIEITL
                     EEAFAGARKSFTMEMDEPCSTCRGAGNVSGQPCASCGGGGWQRSQRTLDVKIPAGVRT
                     GQRVRVSGEGAGAAGVRGDLYLVVTVSAHPFFERSGDEVHLELPITAPEAVLGASVEV
                     PTLRGKVSMKIPPGTSSGRTFRLPGYGMPRVKGGGAGDQLVRAKVVVPSSPTAAEHEL
                     YEKLKALRGDNPRAYMG"
     gene            complement(4561..6033)
                     /locus_tag="VLF19_06270"
     CDS             complement(4561..6033)
                     /locus_tag="VLF19_06270"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSE92892.1"
                     /translation="MKQVRTRTFVVGLAVALAAGVALGGYGVGRAAADRATRPGPAPV
                     VQAPIIPAQMPLNTGTFARVAEVIKPAVININTVSRGGPPGRTPFEEFFGDEFFRRFF
                     GDTPERIPQRSLGSGVIVDPAGVALTNAHVVERATEIEVITLDGSKHKAKVVGMDKKT
                     DVAVLRLDDGKKQFPHARLGDSDQVQVGDWVIAVGSPFGLQSTVTAGIISAKARDLRQ
                     GLFDDFLQTDAAINPGNSGGPLVNMQGEVVGINTAIVAGGSGIGFAIPANMAKRIYTE
                     LLAKGKVTRGWLGVSIQPLTPELAKSFGAKTDKGVLVSDVVPDSPAAKAGVQPGDILL
                     EFEGRKMEGPGDLQRAVGLKNPGTPVKVKVWREQAERTLELKIGEAPDEREARAGSRG
                     GSRSSLGLDVRPVTPELARQLNLRSTEGVVVTRVDEGGAAAEAGVQRGDVIREINRQK
                     VRAMGDFEKLTKDVKEGDRLTLLLQRGPMSLYVAFTVDRG"
     gene            complement(6030..7940)
                     /gene="ftsH"
                     /locus_tag="VLF19_06275"
     CDS             complement(6030..7940)
                     /gene="ftsH"
                     /locus_tag="VLF19_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSE92893.1"
                     /translation="MKPRLSQRLQFSLVYVLVGTVVLLFLQSWLLAPRPVELPMSRFL
                     ELLAEGKIEKVALTEREIHGKARPGVLPAPPSATSVDRIRQALGSEQWQQLFTVTRIP
                     GVDEQWLVRELQQNKVEFAGRIETTFWRDLLFGWVIPLGVMVAIWLFLMRRVGGGPTQ
                     ALSFGRSKHKIYDRKELKTTFADVAGVDEARAELVEIVDFLKNPKKYQRLGGRIPKGV
                     LLVGPPGTGKTLLARAVAGEADVPFFTLSGSEFVEMFVGVGASRVRDLFEQAKDKAPC
                     IVFIDELDAIGKSRAGSTGFVGGHDEREQTLNQLLAEMDGFDSSKGVIIMAATNRPEV
                     LDQALLRPGRFDRQIVVDKPDVKGREAILRLHARAVVLGPDVNLTVIAARTPGFAGAD
                     LANIVNEAALLAARKDKNSVGMSDFEEAIDRVVAGLEKRSRVLSEKERDIVAHHEMGH
                     ALVAFSVAHADPVHKITIIPRGIAALGMTYQLPTEDRFLMTRSELEDRIAVLLGGRVA
                     EELVYGEISTGAHNDLERATELARLMVMKYGMSERLGLATFGDRMSMFLKGPGVPWGG
                     EREYSEETARTIDAEVRDMLDRTHERVTGVLTTKKAVLVAGAAELKRIETLEGERLRR
                     VLAGESVEEMQA"
     gene            8049..9890
                     /locus_tag="VLF19_06280"
     CDS             8049..9890
                     /locus_tag="VLF19_06280"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSE92894.1"
                     /translation="MACDGRNKLVLFTLLAVLLAVVVATLPVQAQDAGKEPAYRSFPL
                     IGSRLAVWAVAQLHLNFAAFILGCPIFAVIIEIVGWRTRNERYDWLAHEFVKLTFAAF
                     SATALLGALLLFLLIGYYPKFWTYMTSIFFPTYWIYALLFFAETFIVYLWYYGWDWLQ
                     GSRKWIHVSLGVAANLAGTAILFVANSWVTFMISPAGVDESGALKGSVWAAITNFTWM
                     PINIHRLIANIVFGGTIAAAYAAFRFLSAETDEERARYDWMGYIGNFVALAAFIVLPF
                     AGYWLGREIYAFNQTMGITMMGGFMSWLWIVQAILIGILFLGSNYYLWLGMERIPGSE
                     RYRRFVPPMLLILTIGFMVWATPRSMVITLDEARAMGGTHHPTLGFFGVMSAKNTVVN
                     LMILTTFLSFVLYRRANRISTRPWVRTGMTIQWAAFAAAAAIVVFYGVYGYFVESIVR
                     IGFSVYQVLAVLACLVLVMAIDIPMFKDARSTGAIRWGTIAPRSQYVLILLAVTFTWL
                     MGLMGFARSGIRQHWHVYGVLRDTSPEAVTPALGYAANMITIVTIVFFALVTFIFWLG
                     GLAEKGKAAGHGHAPAPVIAGSSEGVSTAPSETSPWDRAGRARARTS"
     gene            9890..>10381
                     /locus_tag="VLF19_06285"
     CDS             9890..>10381
                     /locus_tag="VLF19_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSE92895.1"
                     /translation="MRTPVGVKVSVFALAVMGGYSYFANSIPQIESKPPQELSLEGGN
                     VTPAQLVKAGEEIYKTKGTCEVCHRIGQKGTRAPDLAGVGARAAKAKPGTTAKQYLIE
                     SLINPGAFVVEGYPPIMPPVDKPPIALNRSELWALTAFLQSLGGTVDAALNDIPATAG
                     AASA"
BASE COUNT         1408 a         3772 c         3502 g         1699 t
ORIGIN      
        1 cgcgccgcgc ctcgaaccag cgggcgatca gcgccgcgcc cgtcgtcatg gcgaccccgc
       61 cggcgccggc ggtcagcagc agcccgatcg tcacgtagac gtgccagagg tgctggatcg
      121 ccgatgcgct gatgctgccg acgccggcaa ccagcagcga cgcgatgatc acgcgccggg
      181 ggccccagcg atcggccagc cgcccgagga gcgggccgac ggccccgaga agcaggagcg
      241 aagcggcggc ggcgacggac agcgcgccgc gggtccagcc gaactcagtc tccatcggct
      301 tgatgtagac gccgaagacg ttgcgcaggc cggaggccgc catcatgcac agcgcgaggg
      361 cggccaggag gatccaggcg gggtggatcc ggggcggtcg gctcatcccc gtttcgatga
      421 tcgcctccgc tccggcgatt cgcggacgac cgtcagcctc acgggggccc tcccgcctcc
      481 gccgggcgcg caccgagcgc tcgcgggcgc acgaggcacc ccggcgattc gcggacgatg
      541 tgtcggtctc acgagacacg ccttcgcgtt gccgacgcgc gctgggcgct ggtgggttga
      601 cgaagcacgc cacggcgcag catgcgcgtg ccgagcgccg ggagtgtggg gggcgatctc
      661 gggcccccca cgttgtcagc cggcgacttc cgcggcgacc gcgcgcttga acacgatccc
      721 ctcgcctgcc gcgtccacgg tgacggtgtc gccctcgctg aactcgcgct cgaggatctt
      781 gagcgcgagc gggtcctgca cgaggcgctg gatggtgcgc ttgagcggcc gcgccccgaa
      841 ggtcgggtcg tagccctcgc gcgcgagcag cgccttcgcc gcgtcggtga cctccagctc
      901 gatccgccgg tcggccaggc ggcggcggag gtggccgagc tggatctcca cgatccgcgc
      961 cagctccgcg cggccgagcg gcgtgaagac gacgacctcg tcgatccggt tcaggaactc
     1021 cgggcgcagc gtgttgcgca gctccgccat cagcagcggc cgcaccttct cgggctgctc
     1081 gtagtcgcgg aagagctgcg agccgaggtt cgacgtcatg atgacgaccg tgttgcggaa
     1141 gtccacggtc cgtccgtgcc cgtcggtgag ccgcccgtcg tcgaggagct ggagcagcac
     1201 gttgaagaca tcgtggtgcg ccttctcgat ctcgtcgaag agcacgaccg agtagggccg
     1261 ccggcgcacg gcttcggtga gctggccgcc ctcgtcgtac ccgacgtatc cgggcggcgc
     1321 gccgatgagg cgggcgaccg tgtgcttctc catgtactcc gacatgtcca ggcggatcat
     1381 ggcgcgctcg tcgtcgaaga ggaactcggc gagggcgcgc gccagctccg tcttgcccac
     1441 gccggtcggc ccgaggaaca ggaacgagcc gatcgggcgg ttgggatcgc tcaacccgga
     1501 gcgcgcgcgt cgcacggcgt tggcgaccgc gcggatggcc tcgtcctgcc ccaccacacg
     1561 gcggtgcagg cggtcctcca tcgacagcag cttctgcgtc tcgccctcca gcagccgcgt
     1621 gaccggaatg cccgtccact tcgccaccgt ggcggcgatg tcctcctcgt ccacctcctc
     1681 gcgcagcatt cgcgcggtgt cgcccgccgc cggcggctcg ccggtgccgt ccgccgcggc
     1741 cagctgccgc tccagcgtcg ggatcgtgcc gtactggagc tcggccgccc ggttgtagtc
     1801 gccctggcgc atggcatcgg cgagctgctg cctcgcccct tcgagctggg ccttcagcgt
     1861 ggtggcctgg ttgaggccct gcttctcacg ctgccactgc gcgttcagcg cctcgacctt
     1921 cgccttgagt tccgagagct ccgcgtcgat ccgtccgagg cgctcccgtg acgccgggtc
     1981 gtcgtcgcgg gccaccgaca cgcggtcgat ctcgagctgc atgacacggc gctcgatctc
     2041 gtcgatcgcc tggggcttcg agtcgatctg catgcggatg cgcgccgccg cctcgtcgac
     2101 caggtcgatg gccttgtcgg ggaggaagcg atcggcgatg tagcggtgcg acagcacggc
     2161 cgccgcgacg agcgccgcgt ccttgatctt gaccttgtga tgctgctcgt accgctcctt
     2221 caggccgcgc aggatcgaga tggtgtcctc gacgctgggc tcgcggacgg tcacgggctg
     2281 gaagcgccgc tcgagggccg ggtccttctc cacgtgcttg cggtactcgt cgagcgtcgt
     2341 ggcgcccacg cagcgcagct cgccccgggc cagcgccggc ttcagcatgt tggcggcgtc
     2401 cacggcgccc tcggcggcgc cggcgcccac gagcgtgtgc agctcgtcga tgaagcagat
     2461 gatctcgccg ttcgactcga tgatctcctt gagcacggcc ttcagccggt cctcgaactc
     2521 gccccggtat ttcgagccgg ccaccatggc gccgatgtcg agcgccacca ggcgcttgtt
     2581 cttcaggccc tccggcacgt cgccggcgac gatgcgctgg gccaggccct ccacgatcgc
     2641 cgtcttgccg acgccgggct cgccgatgag cacggggttg ttcttcgtcc gccgcgagag
     2701 cacctggatg acgcggcgga tctcctcgtc gcggccgatc acgggatcga gcttgtgctt
     2761 gcgcgcggcc tcggtgaggt cgcgcgagta gcgctggagc gcctggtact tgccctcggg
     2821 gttctcgtcg gtcacgcgct gggtgccgcg cacgtcctgg agcgcgcgat agatcgcgtc
     2881 cttggtcacg ccgttggccg tcagcacgcg cgccgcggcg ccgtcaccgt ccgcgagggc
     2941 gacgagcagg tgctccgcgg acacgtactc gtccttcagc cggtcggcct cgctctgcgc
     3001 gcgctcgaag acgccgagga ctcgctgccc cgcatactgc cccgccgcgc cccggacctt
     3061 gggcatccgc gcgagctccg cctcgagcgt ggcctgcagc gtctccggcc gcgcgccgag
     3121 cttggcgagc agggggccca ccacgccctc gcgctgctcg aggagcgcga gcaggagatg
     3181 ctccggctcg atggcctgat gatcctgccg ctcggcgatc gcctgtgccg cctggaccgc
     3241 ttcccgcgcc ttgaccgtgt acttgtcgaa tctcggtgcc atgtgtcccc tcagcccatg
     3301 tacgcgcgcg ggttgtcacc gcgcagcgcc ttcagtttct cgtacagctc atgctccgcc
     3361 gccgtcgggc tcgacgggac gacgaccttc gcgcgaacga gctggtcgcc ggcgccgccc
     3421 cccttgaccc gcggcatgcc gtagccgggc aggcgaaacg tgcgcccgct cgacgtcccc
     3481 ggcgggatct tcatcgagac cttgccgcgg agcgtcggca cctccacgct ggcgccgagc
     3541 acggcctcgg gcgccgtgat ggggagttcg aggtggacct cgtcgccgct gcgctcgaag
     3601 aacgggtgcg cggagaccgt caccaccagg tagaggtcgc cgcggacgcc ggccgcgccc
     3661 gcgccctcgc cggagacccg gacgcgctgg ccggtgcgga cgcccgcagg gatcttgacg
     3721 tcgagcgtgc gctgcgagcg ctgccacccg ccgccgccac agctcgcgca aggctgcccg
     3781 ctcacgtttc cggccccgcg gcaggtcgag cagggctcgt ccatctccat cgtgaagctc
     3841 ttccgcgcgc cggcgaacgc ctcctcgagc gtgatctcga tggcggcctg cacgtcctcg
     3901 ccgcgggcca cccgcgggcg gcctgcgccc cgccggaagg cgcccccgag caggtcgccg
     3961 aggtcgaagc ctgcgtcctg cccggcgccg ccccgcgtcc gcgcgtctcc cgcgccccgc
     4021 gcggcgaagt ccccgaagat cgtgcggaag aagtccgaga aatccccacc ctcgccgtgc
     4081 tcgacgcgga acccaccacc gggcggtggc tgggcgaagc gctgccagtc cgggccgagg
     4141 ctgtcgtagc gccgccgctt ctcgggatcg gacaggacct cgtatgcctc gctgatctcg
     4201 gcgaaccgtc cggccgtatc cttgcccttg gcgacgtccg gatggtactt ccgggccagc
     4261 ttccggtagg cggacttcag cgccttggcg tcggcactcc gctcgacgcc gaggatcttg
     4321 tagtagtcct tgaactcgac ggccatcctg caggaagtgt agcagatgga ttgcgccctg
     4381 ccggctcatg ccccgcgatt tcggtgcgag gcgcgccaca ggccgagcgc gtggcgcggc
     4441 ggcgggcgca ctgcgcccga gctggcgaca ccgttcaaga acttcccgcg cttgggacca
     4501 cgccggcgag gctgaagcct ctcgccggcg gtgcgcttgc ttcggaggct gttcgagcgg
     4561 ctatccgcgg tccacggtga acgccacgta gagcgacatc ggcccgcgct gcaagagcag
     4621 cgtcagccgg tcgccctcct tgacgtcctt ggtgagcttt tcgaagtcgc ccatcgcgcg
     4681 gaccttctgg cggttgatct cgcggatgac gtcgccgcgc tgcacgccgg cctccgccgc
     4741 cgcgccgccc tcgtcgaccc gggtgacgac gacgccctcg gtggacctga gattcagctg
     4801 gcgcgcgagc tccggcgtca cgggccggac gtccaggccc agcgacgagc gcgagccgcc
     4861 gcggctcccg gcacgggcct cgcgctcgtc gggcgcctcg ccgatcttga gctcgagggt
     4921 ccgctcggcc tgctcgcgcc agaccttcac cttcacgggc gttcccgggt tcttgaggcc
     4981 tacggcgcgc tgcaggtcgc ccgggccctc catcttcctg ccctcgaact cgagcaggat
     5041 atcgcccggc tgcactccgg ccttggcggc cgggctgtcg ggcacgacgt cggagacgag
     5101 cacgcccttg tccgtcttgg ccccgaacga cttcgccagc tcgggcgtga gcggctggat
     5161 cgatacgccg agccagccgc gggtgacctt gcccttggcg agcagctcgg tgtagatgcg
     5221 cttggccatg ttggccggga tggcgaagcc gatgcccgag cccccggcga cgatcgcggt
     5281 gttgatgccg acgacctccc cctgcatgtt cacgagcggc ccaccggaat tgccgggatt
     5341 gatcgcggcg tcggtctgga ggaaatcgtc gaacagtccc tggcgcaaat cgcgcgcctt
     5401 ggcgctgatg atgcccgccg tcaccgtgga ctgcagcccg aacggcgagc ccacggcgat
     5461 cacccagtcg cccacctgga cctggtccga gtcgcccagg cgcgcgtgcg ggaactgctt
     5521 cttgccgtcg tccaggcgca ggacggcgac gtcggtcttc ttgtccatcc cgaccacctt
     5581 cgccttgtgc ttgctgccgt cgagcgtgat cacctcgatc tccgtggccc gctcgacgac
     5641 gtgggcgttg gtcagcgcca ccccggcggg gtccacgatg acgccggagc ccaggctgcg
     5701 ctggggaatg cgctccggcg tgtccccgaa gaaccgccgg aagaactcgt cgccgaagaa
     5761 ctcctcgaac ggcgtccgcc ccggcgggcc gccccggctc accgtgttga tgttgatgac
     5821 ggcgggcttg atgacctcgg ccaccctggc gaacgtcccc gtgttcaggg gcatctgcgc
     5881 cgggatgatc ggggcctgga ccacgggcgc cggccccggg cgcgtcgccc ggtcggcggc
     5941 ggcgcgcccc accccgtacc cgccgagcgc gacgccggcc gccagcgcca ccgcgagccc
     6001 gacgacgaag gtcctcgtcc tgacctgctt catgcctgca tctcctccac gctctcgccc
     6061 gcgagcacgc ggcgcagccg ctcgccctcc agggtctcga ttcgcttcag ctcggccgcg
     6121 cccgcgacga gcacggcctt cttggtcgtc aggactccgg tgacccgttc gtgggtccgg
     6181 tcgagcatgt cgcgcacctc ggcgtcgatc gtgcgggccg tctcctcgct gtactcgcgc
     6241 tccccgcccc acggcacccc ggggcccttc aggaacatcg acatgcggtc gccgaacgtc
     6301 gccagcccga gccgctcgga catgccgtac ttcatgacca tgagccgcgc cagctcggtg
     6361 gcgcgctcga ggtcgttgtg ggcgccggtg gagatctcgc cgtagaccag ttcctcggcg
     6421 acccgcccgc cgagcagcac cgcgatccgg tcctccagct cgctgcgcgt catcaggaag
     6481 cggtcctcgg tcggcagctg gtaggtcatg ccgagggcgg cgatgccgcg cggaatgatc
     6541 gtgatcttgt gcacggggtc ggcgtgggcc accgagaagg cgaccagtgc gtggcccatc
     6601 tcgtggtgcg ccacgatgtc gcgctccttc tcggagagca cccggctccg cttctcgagg
     6661 cccgcgacga cccgatcgat cgcctcctcg aagtccgaca tccccacgga gttcttgtcc
     6721 ttgcgggcgg ccagcaacgc cgcctcgttg acgatgttgg cgaggtcggc gccggcgaag
     6781 ccgggcgtgc gcgcggcgat gacggtcaag ttgacgtcgg gaccgaggac gacggcgcgc
     6841 gcgtgcaatc gcaggattgc ctcgcggccc ttgacgtcgg gcttgtccac gacgatctgg
     6901 cggtcgaagc gcccgggtcg gagcagcgcc tggtccagca cctcgggccg gttggtcgcc
     6961 gccatgatga tgacgccctt ggacgagtcg aagccgtcca tctcggcgag cagctggttg
     7021 agcgtctgct cgcgctcgtc gtgaccgccc acgaaccccg tcgagcccgc ccgcgacttg
     7081 ccgatcgcgt cgagctcgtc gatgaagacg atgcacgggg ccttgtcctt ggcctgctcg
     7141 aagagatcac gtacgcggga ggcgccgacg cccacgaaca tctccacgaa ctccgagccc
     7201 gagagcgtga agaacggcac gtcggcctcg cccgccaccg cgcgggccag cagggtcttc
     7261 ccggtgccgg gcggacccac gagcagcacg cccttgggga tccgcccgcc gaggcgctga
     7321 tacttcttgg ggttcttgag gaagtccacg atctcgacca gctcggccct ggcctcgtcg
     7381 acgccggcca cgtcggcgaa ggtcgtcttc agctccttgc ggtcgtagat cttgtgcttg
     7441 ctccgcccga acgacagcgc ctgggtcggc ccgcccccca cgcggcgcat caggaacagc
     7501 cagatggcga ccatgacgcc gagcgggatg acccagccga acagcagatc gcgccagaac
     7561 gtcgtctcga tgcgtccggc gaactcgacc ttgttctgct ggagctcccg gacgagccat
     7621 tgctcgtcca cgccgggaat ccgcgtgacc gtgaagagct gctgccactg ctccgagccc
     7681 agcgcctggc gtatccggtc gaccgaggtc gcggacggcg gcgccggcag cacgccgggc
     7741 ctggccttgc cgtggatctc gcgctcggtc aacgccacct tctcgatctt gccctcggcg
     7801 agcagctcga ggaacctcga catgggcagc tcgacggggc gcggggcgag cagccacgac
     7861 tggaggaaga gcaggacgac ggtcccgacc aggacgtaga cgagggagaa ctgcaggcgc
     7921 tgggacagtc gcggcttcat ggaaggcccc tcgtttccga tgctagcgcc cggggtgggg
     7981 agtgtcaaac gtgacgatcc catttcccct tgacactccc ggtcctttga cctacaaaga
     8041 gccccgaaat ggcatgtgac gggcggaaca agcttgtctt gttcaccctt ctcgccgtcc
     8101 tcctcgcggt ggtcgtggcg acgctcccgg tgcaggcgca ggacgccggg aaggagcccg
     8161 cctaccggtc gttcccgctg atcggctccc gcctggcggt gtgggcggtg gcccagctcc
     8221 acctcaactt cgccgccttc atcctggggt gcccgatctt cgccgtcatc atcgagatcg
     8281 tcggctggcg aacgcgaaac gaacgctacg actggctggc ccacgagttc gtcaagctaa
     8341 cgttcgccgc cttctcggcg accgccctgc tgggcgccct gctcctgttc ctcctgatcg
     8401 ggtactaccc gaagttctgg acgtacatga cgtccatctt cttccccacc tactggatct
     8461 acgcgctgct gttcttcgcc gagaccttca tcgtctacct ctggtactac gggtgggact
     8521 ggctgcaagg atcgcgcaag tggatccacg tctcgctcgg cgtggccgcc aacctcgccg
     8581 gcaccgccat tctcttcgtc gccaactcgt gggtgacgtt catgatctcg ccggcggggg
     8641 tggacgagtc aggggcgctc aaggggagtg tctgggcggc gatcaccaac ttcacgtgga
     8701 tgcccatcaa catccaccgg ctcatcgcca acatcgtgtt cggcgggacg atcgccgccg
     8761 cgtacgccgc cttccggttc ctcagcgccg agacggacga ggagcgggcc cgctacgact
     8821 ggatgggcta catcggcaac ttcgtggcgc tggccgcgtt catcgtgctg cccttcgccg
     8881 gctactggct ggggcgcgag atctacgcgt tcaaccagac gatgggcatc acgatgatgg
     8941 gcggcttcat gtcgtggctg tggatcgtgc aggccatcct catcggcatc ctgttcctcg
     9001 gctccaacta ctacctgtgg ctggggatgg agcgcatccc gggctccgag cgctatcgga
     9061 ggttcgtgcc gccgatgctg ctgatcctca ccatcggctt catggtctgg gccacgcccc
     9121 gctcgatggt gatcacgctc gacgaggcgc gcgcgatggg cggcacccac cacccgacgc
     9181 tgggcttctt cggcgtcatg tcggcgaaga acaccgtcgt caacctcatg atcctcacca
     9241 cgttcctgtc cttcgtgctc taccgccggg cgaaccggat ctcgactagg ccctgggtgc
     9301 gcaccggcat gaccatccag tgggcggcct tcgcggccgc cgccgcgatc gtcgtcttct
     9361 acggcgtcta cgggtacttc gtcgagtcca tcgtgcgcat cgggttctcc gtctaccagg
     9421 tcctggccgt gctcgcctgc ctcgtcctcg tgatggccat cgacatcccc atgttcaagg
     9481 acgcccgctc gaccggggcc atccgctggg gcacgatcgc gccgcgctcg cagtacgtgc
     9541 tgatcctgct cgcggtgacg ttcacctggc tgatggggct catgggcttc gcgcggtcgg
     9601 gcatccggca gcactggcac gtctacgggg tgctgcgcga cacgtcgccc gaggcggtca
     9661 cgccggccct ggggtacgcc gccaacatga tcaccatcgt gacgatcgtc ttcttcgccc
     9721 tcgtcacgtt catcttctgg ctcggcggcc tcgccgagaa gggcaaggcc gcgggacatg
     9781 gccacgcccc cgcgccggtc atcgccggct cctcggaagg ggtctcgacg gccccctccg
     9841 agacctcccc ctgggatcgc gcgggccgag cccgcgctcg aacgagctag tgcggacccc
     9901 ggtaggcgtg aaggtgagcg tcttcgccct ggcggtcatg ggcggctaca gctacttcgc
     9961 gaactccatc cctcagatcg aatcgaagcc gccgcaggag ctgtcgctgg agggtggcaa
    10021 cgtgaccccg gcgcagctcg tgaaggccgg cgaggagatc tacaagacga agggcacctg
    10081 cgaggtctgt caccgcatcg gccagaaggg cacgcgggcc cccgatctcg ccggggtggg
    10141 ggcgcgcgcc gcgaaggcca agcccggcac gaccgccaag cagtacctca tcgagtcgct
    10201 gatcaacccc ggcgccttcg tcgtcgaggg ctatccgccg atcatgccgc cggtggacaa
    10261 gccgccgatc gcgctcaacc gctcggagct ctgggccctc acggccttcc tgcagtccct
    10321 cggcgggacc gtggacgccg cgctgaacga catcccggcc acggccgggg cggcgagcgc
    10381 t
//
LOCUS       DATIAZ010000176         3127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1755195, whole genome shotgun sequence.
ACCESSION   DATIAZ010000176 DATIAZ010000000
VERSION     DATIAZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755195"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="VLF19_06290"
     CDS             <1..2017
                     /locus_tag="VLF19_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSE92896.1"
                     /translation="PPVNALSQHVRQGLRDGLTRALADQQAGAVLIVCAGRTFIAGAD
                     ITEFGKPPKEPSLHEVLDRIEGSPKPVIAAVHGTALGGGLEVTLACHYRVAVKTARFG
                     LPEVKLGLLPGAGGTQRLPRVVGAPKALQMITSGDPIGADEALKHGLIDEIVDGDLAA
                     AGVVFAEKVLAEKRPLRKIRDMDDKVAAVRGKPEVFAEFRKSIARQTRGFRAPESCVK
                     AVEAAVNLPFAEGLARERELFVELLNSPESRAQRYFFFAEREAAKIPDVPADTPAKDI
                     KKAAVIGAGTMGGGIAMNFANVGIPVAVVEMNQEALDRGLGVVRKNYEATASRGRLTP
                     ADVEKRMALIHGTTDWNAVRDADIVIEAVFEEMPVKKEVFAKLDGLAKADAVLATNTS
                     TLDVNEIASATRRPESVIGTHFFSPANVMRLLENVRGAKTSKTTVATAMAVGRRINKV
                     PVLVGVCYGFVGNRMLHQRGQQAEKLILEGAAPHQVDRVLTDFGFPMGPFAMGDLAGL
                     DVGWRIRKGRGVKAPVADRICELGRFGQKTGAGYYRYEKGDRTPIPDADVEKIIVDVA
                     TSMGITRRPISDEEILQRLLYPMVNEGAKILDEKIAIRASDIDVIWVYGYGWPVYRGG
                     PMYWADQIGLGAVRDTLLAFQRATGDTFWTPAPLLAKLADEGKAFTG"
     gene            2031..>3127
                     /locus_tag="VLF19_06295"
     CDS             2031..>3127
                     /locus_tag="VLF19_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE92897.1"
                     /translation="MTASESLASPRLWLAFATMLVVSGIGNTFPVFFPALLEEFGGSR
                     AATAATVSLVWLVGAALGPVAGHLVDRWSPRLLVVAGLGATALGMAGAIVAPTLPLFV
                     LALGVGGGVGVGLSGMVTQAAVIAETYVRRRGFATGIAFSGSMAGYVLSTPAQSAIEA
                     LGWRATLAGYLVVVLILIPFAAAIYPARLAARVVTAPAAAPPAGTRTMWTLVRTPAFW
                     ALAFVFTVAPLVGYLATLQHALYFASLGFPAHEAAIMLTLGGVLSTSGRALVGLVADR
                     FGAPLAGVASLSMSLLGTLCLAGLEAWPGRVLAYAYVLLVFGPLGTRAVVVSLLVPRL
                     AAPARFGAVFGVLVMVNSAGAGLGPFLSGAI"
BASE COUNT          414 a         1147 c         1102 g          464 t
ORIGIN      
        1 cccgcccgtg aacgcgctga gccagcacgt gcggcagggg ctgcgcgacg ggctcactcg
       61 agccctcgcc gatcaacagg cgggcgccgt cctgatcgtc tgcgccggcc gcacgttcat
      121 cgccggcgcc gacatcacgg aattcggcaa gccgccgaag gagccgtcgc tccacgaggt
      181 gctcgatcgc atcgagggct cgcccaagcc cgtcatcgcc gcggtccacg gcaccgcgct
      241 cggtggcggc ctcgaggtga cgctggcgtg tcactaccgc gtggccgtga agacggcgcg
      301 gttcggcctg cccgaggtga agctcggcct cctgcccggc gccggcggta cccagcggct
      361 gccgcgcgtg gtcggcgcgc cgaaggcgct ccagatgatc accagcggcg acccgatcgg
      421 cgccgacgag gccctgaagc acggcctgat cgacgagatc gtggacggcg acctcgccgc
      481 ggccggcgtc gtcttcgccg agaaggtgct cgccgagaag cgcccgctgc gaaagatccg
      541 cgacatggac gacaaggtgg cagccgtgcg cggcaagccg gaagtcttcg ccgagttccg
      601 caagtcgatc gcgcggcaga cccgcggctt ccgggcgcca gagagctgcg tgaaggccgt
      661 cgaggccgcc gtcaacctgc ccttcgccga gggcctcgcg cgggagcgcg agctcttcgt
      721 cgagctgctg aactcgccgg agtccagggc ccagcgctac ttcttctttg ccgagcgcga
      781 ggccgccaag atccccgacg tgcccgccga cacgccggcg aaggacatca agaaggccgc
      841 cgtcatcggc gccggcacca tgggcggcgg catcgcgatg aacttcgcca acgtgggcat
      901 cccggtcgcg gtcgtcgaga tgaaccagga ggcgctcgac cggggcctcg gcgtcgtccg
      961 gaagaactac gaggcgactg ccagccgggg ccggctcacg cccgccgacg tcgagaagcg
     1021 catggcgttg atccacggca cgacggactg gaacgccgtg cgcgacgccg acatcgtgat
     1081 cgaggccgtg ttcgaggaga tgcccgtcaa gaaggaagtc ttcgcgaagc tggacgggct
     1141 cgcgaaggcc gacgcggtgc tggccacgaa cacctcgacg ctcgacgtca acgagatcgc
     1201 ctcggccacc cggcggccgg agagcgtgat cggcacgcac ttcttcagcc cggccaacgt
     1261 catgcggctg ctcgagaacg tgcgtggcgc gaagacctcg aagacgacgg tcgccaccgc
     1321 gatggccgtt ggccggcgga tcaacaaggt gccggtgctc gtcggcgtct gctacggctt
     1381 cgtcggcaat cgcatgctcc accagcgcgg ccagcaggcc gagaagctca tcctggaggg
     1441 cgcggcgccg caccaggtgg acagggtgct caccgacttc ggcttcccca tgggcccgtt
     1501 cgccatgggc gacctcgccg gcctcgacgt gggctggcgc atccgcaagg ggcgcggcgt
     1561 gaaggcgccg gtggccgacc gcatctgcga gctggggcga ttcggccaga agaccggcgc
     1621 cggctactac cgctacgaga agggcgaccg cacgccgatc cccgatgccg acgtcgagaa
     1681 gatcatcgtc gacgtcgcca cgtccatggg catcacgcgg cggcccatct ccgacgagga
     1741 gatcctccag cgactgctct acccgatggt gaacgagggc gccaagatcc tcgacgagaa
     1801 gatcgccatc cgcgccagcg acatcgacgt gatctgggtc tacggctacg gctggccggt
     1861 gtaccgcggc ggcccgatgt actgggccga ccagatcggg ctcggcgcgg tccgcgacac
     1921 gctcctcgcg ttccagcgcg ccaccggcga caccttctgg acgccggcgc cgctgctcgc
     1981 gaagctcgcc gacgagggca aggcgttcac cgggtagcga ccgcggtccg gtgaccgcgt
     2041 ccgagagcct cgccagcccg cggctctggc tcgcgttcgc gacgatgctc gtggtctcgg
     2101 gcatcggcaa cacgttcccg gtcttcttcc cggcgctgct cgaggagttc ggaggctcgc
     2161 gcgcggcgac ggcggcgacg gtcagcctcg tctggctcgt cggcgccgcg ctcgggcccg
     2221 tcgccggcca tctcgtcgac cgctggagcc cccgcctgct cgtcgtggct ggccttggcg
     2281 cgacggcgct gggcatggcc ggcgccatcg tcgcgcccac cctgcccctc ttcgtgctcg
     2341 cgctcggcgt cggcggcggc gtcggcgtcg gactcagcgg catggtgacg caggccgcgg
     2401 tgatcgccga gacctacgtg cggcgccgcg gcttcgccac cggcatcgcc ttctcgggct
     2461 cgatggcggg ctacgtgctc tcgacgcccg cacagtcggc gatcgaggcg ctcggctggc
     2521 gggcgacgct cgccggctac ctcgtggtcg tgttgatcct gatccccttc gcagccgcca
     2581 tctatccggc gcgcctggcc gcccgggtcg tcacggcccc ggcagcggca ccgccggcgg
     2641 gcacgcgcac catgtggacg ctcgtgcgca cgcccgcgtt ctgggcgctc gccttcgtgt
     2701 tcacggtggc tccgctcgtc ggctacctcg ccacgctgca gcacgcgctc tacttcgcct
     2761 cgctcggctt tcccgctcac gaggccgcca tcatgctgac gctcggcggc gtgctctcga
     2821 cgtcgggacg ggccctcgtc ggcctcgtcg ccgaccgctt cggcgcgccg ctggcgggcg
     2881 tggcgtcgct gtcgatgtcg ctgctgggca ccctgtgcct ggccggcctc gaggcgtggc
     2941 ccggacgcgt gctcgcctac gcttacgtgt tgctggtgtt cgggccgctg ggcacgcggg
     3001 cggtggtcgt ctcgctgctg gtcccccgcc tcgcggcgcc ggcgcgcttc ggcgccgtct
     3061 tcggcgtcct cgtcatggtc aacagcgccg gcgccggcct gggcccgttc ctctcgggcg
     3121 cgatcta
//
LOCUS       DATIAZ010000177         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1885507, whole genome shotgun sequence.
ACCESSION   DATIAZ010000177 DATIAZ010000000
VERSION     DATIAZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885507"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VLF19_06300"
     CDS             complement(<1..1055)
                     /locus_tag="VLF19_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE92898.1"
                     /translation="MARAYNVIDADGHVLEPVDLWDKYMDPAYRDRAPRLIVDTDGKE
                     RLLIEGRVLGSPKGMGVIGAIGARDGTVPEATMKYVEGRPGGFDPHARIPDMDLDGID
                     AAFLYPSLGLFAGAIKDPALAAAACRAYNRWLADYCRPYPDRLFGVAMLPMQSIELAI
                     EEMRFARKELGMRGGFLRPNPYNGRMLHHPDYAPFWAEVQELDFSIGLHEGASGGMPQ
                     VGVDRFETRGARHIISHTMEMMLAAMSVIWGGVCDRYPRVRIAFLESGGGWIAPWLDR
                     MDRHFDDQGFNDSGLSMRPSELFQRNCWISFEPVEGCLSVLADYIGPHKILWATDYPH
                     PDGFFPGAPKLIADRPEL"
     gene            complement(1239..2435)
                     /locus_tag="VLF19_06305"
     CDS             complement(1239..2435)
                     /locus_tag="VLF19_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92899.1"
                     /translation="MPKALDGIRVLDLTQYEAGPSCTQMLAWLGADVIKIEPPTGEPG
                     RTALSDKRGEDAWFFLLLNSCKKGVTLNLKSPRGRAMFEELAKTADVVVENMGPGAME
                     RLGLGYEALKRLNPRIIAASVKGFGSGGPYSDYKSFEWIAQAMAGAMSMTGSPDGPPT
                     KAIGGLADTGAGLHTAIGILAAIIQRQTTGVGQQIEVAQQDSVVNLLRIHLRETYATG
                     KPAPRQGNRSSAAAPSNLYRCRPGGMNDYVFVHCATVEMWKSLTALVGRPELGDDPRY
                     ADRRDRVEFIDEIDAMIEAWTEKRTKHEVLEIFAGAGIPCGAVLDSSEVLSDPHLRQR
                     RFIVDLEHPRRGAYPMPGNPVRLSDSPTDVVRAPLLGEHNSEVYGTLLGYGPAELDAL
                     RRDGVV"
     gene            complement(2624..3982)
                     /locus_tag="VLF19_06310"
     CDS             complement(2624..3982)
                     /locus_tag="VLF19_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSE92900.1"
                     /translation="MSDREAAFVAGLDARVAHILESCSRCGRCVEVCPTAGPAGIDTR
                     DPASIVSEVLDVLRGLGVTGSRGARWAETCTGSGRCIGACDDGVNPRFMLAMTRVRLN
                     ERKAETERQATGQKAFHTMSQGVRVLSRLQLPPAFVGKVTRSAHTGERSAADVVMYLG
                     CNVLKTPHIALLCLDVLDRIGTTCMVLGGPANCCGVIQFRAGDTRTAGRIGGNTVAGF
                     AGTGIPRVLTWCPTCNIQLGEIVMPSTNPGFALEHVVPHIADRLDRLTPHFVRPVPKR
                     VALHEHPGVAGVTEGVMKILGAIPGLELVDLAQPRVGYMCNSLAPVADYKRELHAREL
                     AAAEAAGVDCLVGIYHACHRELCAHERDYPFRIVNFLELVGEAMGVERQDLFKQWKVM
                     QDVDRVLAEVATEASAAGLDAETVRQVMVGAITGEQPLPLGQREARRSVASPGPGSFF
                     PE"
     gene            complement(3979..>4629)
                     /locus_tag="VLF19_06315"
     CDS             complement(3979..>4629)
                     /locus_tag="VLF19_06315"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HSE92901.1"
                     /translation="FEGITALDAIGPYEVLSRLPGARARFVALAPGTYRTDNRMLALV
                     ADEALEAVPHPKIVLVPGGFGTRALMTNERLLDWIRAAHETSRWTTSVCTGSLLLAAA
                     GLLRGLEATTHWMSLERLAEFGARPVQRRVVEQGKVITAAGVSSGIDMALALAARIAG
                     DEVAQAIQLGIEYDPQPPFSAGSPRTAPAHVVERVRQRMQARLDAQAVPTSSADAR"
BASE COUNT          651 a         1661 c         1653 g          664 t
ORIGIN      
        1 agctccgggc ggtcggcgat cagcttcggc gcgccgggaa agaagccgtc cgggtgcgga
       61 tagtcggtgg cccacaggat cttgtgcggg ccgatgtagt ccgcgagcac gctgaggcat
      121 ccctcgacgg gctcgaacga gatccagcag ttccgctgga acagctcgct cgggcgcatg
      181 gagagccccg agtcgttgaa gccctggtcg tcgaagtgcc ggtccatgcg gtcgagccag
      241 ggcgcgatcc accccccgcc ggactccagg aatgcgatgc gcacgcgcgg gtagcggtcg
      301 cacacgccgc cccagatgac gctcatggcc gcgagcatca tctccatcgt gtgcgagatg
      361 atgtgccggg ccccgcgcgt ctcgaagcgg tcgacgccga cctgcggcat gccgccgctg
      421 gcgccctcgt gcaggccgat ggagaagtcc agctcctgca cctccgccca gaacggcgcg
      481 tagtccgggt ggtggagcat ccgcccgttg tacgggttgg ggcggaggaa gcccccgcgc
      541 atgccgagct ccttccgggc gaagcgcatc tcctcgatgg ccagctcgat cgactgcatc
      601 ggcagcatgg ccacgccgaa gaggcgatcg ggatagggcc ggcaatagtc ggcgagccag
      661 cggttgtagg cccggcacgc cgccgcggcc agcgcgggat ccttgatggc tccggcgaag
      721 aggcccaggc tcggatagag gaacgcggcg tcgatgccgt ccaggtccat gtccgggatc
      781 cgggcgtggg ggtcgaagcc gcccgggcgg ccctcgacgt acttcatggt ggcctcgggc
      841 accgtgccgt cgcgggcgcc gatcgcgccg atgacgccca tgcccttcgg gctgccgagc
      901 acccggccct cgatgagcag gcgctctttg ccgtcggtgt cgacgatgag ccgcggcgcg
      961 cggtcgcgat aggccggatc catgtacttg tcccacaggt ccaccggctc gagcacgtga
     1021 ccatcggcat cgatgacgtt gtacgcgcgc gccatgctca gtctcctctc ccggaccacc
     1081 ccggctcccg cccggccgtt cgaacaccct ggggcgatgt ccggcctccc aaaccgccgc
     1141 gggagccgag gaccgccacg gctccgccgg ggcggtccga cccgcccccg ggaggcgggg
     1201 ccatctcggg gcccccatgt aatcgatcca tcaatctatc aaacgacgcc gtcgcgccgc
     1261 agggcgtcga gctccgccgg cccgtagccg agcagcgtgc cgtagacctc cgagttgtgc
     1321 tcgccgagca gcggcgcgcg aacgacgtcc gtcggcgagt ccgacagccg cacggggttg
     1381 cccggcatcg ggtacgctcc gcgccgcggg tgctcgaggt cgacgatgaa gcgccgctgc
     1441 cgcaggtggg gatccgacag gacctcgctc gagtcgagca cggcgccgca cgggatgccg
     1501 gcgccggcga aaatttccag cacctcgtgc ttggtccgtt tctccgtcca cgcctcgatc
     1561 atcgcatcga tctcgtcgat gaactccacg cggtcgcgcc ggtcggcgta gcgcgggtcg
     1621 tcgcccagct ccggccggcc cacgagcgcc gtgaggctct tccacatctc gacggtggcg
     1681 cagtggacga acacgtagtc gttcatgccg ccgggacggc agcggtagag gttggacggt
     1741 gccgcggccg acgagcggtt gccctgccgg ggcgcgggct tgccggtggc gtaggtctcg
     1801 cgcaggtgga tccggagcag gttcaccacc gagtcctgct gggcgacctc gatctgctga
     1861 ccgacacccg tcgtctggcg ctggatgatc gccgccagga tgccgatcgc cgtgtgcagc
     1921 ccggcgcccg tgtcggccag gccgccgatg gccttggtgg gcgggccgtc cggagatccc
     1981 gtcatgctca tggccccagc catggcctgg gcgatccact cgaaggactt gtagtccgag
     2041 tacggcccgc cgctgccgaa gcccttgacc gacgcggcga tgatgcgtgg gttcaggcgc
     2101 ttgagcgcct cgtagccgag accgaggcgc tccatcgcgc ccggccccat gttctcgacc
     2161 acgacgtccg cggtcttcgc gagctcctcg aacatcgcgc ggccgcgcgg cgatttcaga
     2221 ttgagcgtga cgcccttctt gcaggagttc agcagcagga agaaccacgc gtcctcgcca
     2281 cgcttgtcgg agagcgccgt ccggccgggc tcgccggtcg gcggctcgat cttgatgacg
     2341 tcggcgccga gccacgccag catctgtgtg caggatgggc cagcctcgta ctgggtcagg
     2401 tcgagcacgc ggatcccgtc gagcgccttc ggcatcgcct cgttctcccc gtctgttcgt
     2461 gtcaactcgg cgggcctccg cgccgcagcg tgtcgcgcgg ccgagcgccc accctcacag
     2521 cgacgtcagg agcgcgctgc gtggtgcgcc gcagcccggg gcgcgtccgc gtcatcgtgg
     2581 acgctcccct gtcggcgtcg ccggcgggct ccagtgcacg gcgtcactcc gggaagaacg
     2641 atcccggacc cggcgaggcc accgaccggc gcgcctcgcg ctggccgagc ggcagcggct
     2701 gctcgcccgt gatcgcgccg accatgacct ggcgcaccgt ctcggcgtcg aggccggcgg
     2761 ccgacgcctc ggtggccacc tcggccagga cgcggtccac gtcctgcatg accttccact
     2821 gcttgaacag atcctggcgc tcgacgccca tcgcctcgcc gaccagctcg aggaagttca
     2881 cgatcctgaa ggggtagtcg cgctcgtgag cgcacagctc ccggtggcag gcgtggtaga
     2941 tgccgaccag gcaatcgacg ccagccgctt ccgcggcggc gagctcccgc gcgtgcagct
     3001 cgcgcttgta gtcggccacc ggcgccagcg agttgcacat gtagccgacg cgcggctggg
     3061 cgagatcgac cagctccagg ccggggatgg cgccgaggat cttcatcacc ccctcggtca
     3121 cgccggccac gcccgggtgc tcgtggagcg ccacgcgttt gggcaccggg cggacgaagt
     3181 gcggcgtgag gcggtccagg cggtcggcga tgtggggcac cacgtgctcg agggcgaagc
     3241 cggggttggt cgagggcatc acgatctcgc cgagctggat gttgcaggtc gggcaccacg
     3301 tgagcacgcg cgggatgccg gtacccgcga acccggccac ggtgttgccg ccgatgcggc
     3361 cggccgtgcg cgtgtcgccg gcgcggaact ggatgacgcc gcagcagttg gcggggccgc
     3421 ccagcaccat gcaggtcgtc ccgatgcggt cgaggacgtc caggcacagc agcgcgatgt
     3481 gcggggtctt gagcacgttg cagccgaggt acatgacgac gtcggcggca gaccgctcac
     3541 cggtatgggc cgagcgcgtg accttgccga cgaacgcggg cggcaactgc agccgcgaca
     3601 gcacgcggac gccctgggac atcgtgtgga aggccttctg cccggtcgcc tggcgctcgg
     3661 tctctgcctt gcgctcgttc agcctcaccc gcgtcattgc cagcatgaat cgcggattca
     3721 cgccgtcgtc gcaggcgccg atgcagcggc cggagccggt gcacgtctcg gcccagcgcg
     3781 ccccgcggct tccggtcacg cccaggccgc gcagcacgtc gagcacctcg gagacgatcg
     3841 acgccgggtc gcgcgtgtcg atgcccgccg ggccggcggt gggacagacc tcgacgcagc
     3901 gcccgcagcg cgagcacgac tcgaggatgt gcgcgacccg cgcgtcgagg cccgccacga
     3961 acgccgcttc gcggtcactc atcgggcatc agccgaggac gtcgggaccg cctgggcgtc
     4021 cagccgcgcc tgcatccgct gccggacgcg ctccaccacg tgcgcgggcg cggtccgcgg
     4081 tgagcccgcc gagaacggcg gctggggatc gtactcgatg ccgagctgga tggcctgggc
     4141 cacctcgtcg ccggcgatcc gcgccgcgag cgcgagcgcc atgtcgatcc cggacgacac
     4201 gcccgcggcg gtgatgacct tgccctgctc gaccacgcgt cgctggaccg gccgcgcgcc
     4261 gaactcggcc agccgctcga gcgacatcca gtgcgtggtg gcctcgagcc cgcgcaggag
     4321 gccggccgcg gccaggagca gcgagcccgt gcagaccgac gtggtccacc ggctcgtctc
     4381 gtgggccgca cggatccagt cgagcagccg ctcgttcgtc atgagcgcgc gggtcccgaa
     4441 gccgccgggc acgagcacga tcttcggatg cggaaccgcc tcgagcgcct cgtcggccac
     4501 cagcgcgagc atgcggttgt ccgtgcggta cgtgcccggc gcgagggcca cgaaccgcgc
     4561 gcgggcgccg ggcaggcgcg agagcacctc gtaggggccg atggcgtcca gtgccgtgat
     4621 gccctcgaa
//
LOCUS       DATIAZ010000178         2168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1755307, whole genome shotgun sequence.
ACCESSION   DATIAZ010000178 DATIAZ010000000
VERSION     DATIAZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755307"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VLF19_06320"
     CDS             complement(<1..257)
                     /locus_tag="VLF19_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HSE92902.1"
                     /translation="MTCACGSFTSGPVVLRDVALFTDLYELTMAASYLREGMTREATF
                     SLFVRKLPRGRSFLVAAGLEDVLALLEQFQFSEASLRYLRSL"
     gene            complement(416..>2168)
                     /locus_tag="VLF19_06325"
     CDS             complement(416..>2168)
                     /locus_tag="VLF19_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSE92903.1"
                     /translation="SLAVSAIPEGLPAVTAVALAAGLWRLARAGALVRRLPAVETLGA
                     TTVICSDKTGTMTENRMAVARVWLPGRVVEVGEPGTSRHGRFSERGRPIAPLEDSDLV
                     RLLTVAALVNDASVEAEGDGRRLHGDPTEAALLVAALEAGLDVTALSSAWPRRGEIPF
                     SPVARLMATFHETPEGGHAVLVKGAPGAVLERCERVHAAGTLRRVDDETRRRLLDDNR
                     ALANEGYRVLAVAWRDEAAPEDRHIEDLTFLGFVALADPLRAGVKEALAQCREAGIAI
                     VMLTGDQRTTAEAIGRQLGLAADAIRSRVGPEDKLELIKALQARGEVVAMTGDGVNDA
                     PALVRADIGIAMGRHGTDVAREAADVVLTDDNFATIVGAVQEGRVIYGNLKKVIRFLF
                     SCNLSEIVAIFAAILAGFPSPLTPLQILWVNVVTDILPAMALVRDPAEPDVMRRPPRD
                     PGEALVTWRLGTRMLMEAAWLAAGVLSAYFWIVLQEGPGPRAHTVAFVALVLIHPFQA
                     LHCRSERVFWWRLPPNRLVWASMLALVAVQWLAVTWPPLAGLLGAVPLTAGDWLVTIV
                     AVLWPVSLLEATKRWRG"
BASE COUNT          339 a          778 c          786 g          265 t
ORIGIN      
        1 agcgagcgaa gatagcggag ggaggcctcc gagaactgga actgctcgag cagggcgagg
       61 acgtcctcga gccccgcggc caccaggaac gagcggccgc gcggcagctt gcggacgaac
      121 aggctgaagg tcgcctcccg ggtcatcccc tcccgcagat aggaggccgc catggtcagc
      181 tcgtacaggt cggtgaacag ggcgacgtcg cggaggacga cgggccccga cgtaaaggat
      241 ccacaggcgc aggtcatggc gtgtccacgg ccgggcgagt gcaagccgtc cgccaagtcg
      301 gcacggggct ttccggccgt gatttccagt ccctacgagc cggcggggcg acggccaacc
      361 gatccgttgc gccgccgcgc ctaccccact ggggcgaagc tgggacaccg gcgcgtcacc
      421 cccgccaacg cttggtcgcc tcgagaaggc tgaccggcca cagcaccgcg acgatggtga
      481 cgagccagtc gccggcggtg agcggcacgg cgcccagtag tccggctagc ggaggccacg
      541 tgacggcgag ccactgcacg gcgacgaggg ccagcatcga ggcccagacc aggcgattgg
      601 gcggcaggcg ccaccagaag acgcgctcgg accggcagtg cagcgcctgg aacgggtgga
      661 tgagcacgag cgcgacgaag gccaccgtgt gggcgcgtgg ccccgggccc tcctgcagga
      721 cgatccagaa gtacgcgctc aggacaccgg cggcgagcca cgccgcctcc atgagcatcc
      781 gcgtgcccag gcgccacgtc acgagcgcct cgcccgggtc gcgcggggga cggcgcatca
      841 cgtcgggctc cgcggggtcg cgtacgagcg ccatcgcggg caggatgtcg gtcacgacgt
      901 tgacccacag gatctgaagc ggcgtcagcg ggctcgggaa cccggcgagg atggccgcga
      961 agatcgcgac gatctccgac aggttgcacg agaacaggaa ccggatcacc ttcttcaagt
     1021 ttccgtagat gacccggccc tcctgcaccg cgccgacgat cgtggcgaag ttgtcgtcgg
     1081 tcagcacgac gtcggcggcc tcgcgcgcca cgtcggtgcc gtggcgcccc atcgcgatac
     1141 cgatgtccgc acggaccagc gccggcgcat cgttcacgcc gtcaccggtc atcgccacga
     1201 cctctccgcg cgcctggagc gccttgatca gctcgagctt gtcctcgggg cccacgcggc
     1261 tccggatcgc gtcggcggcc agacccagct gccgtccgat ggcctcggca gtcgtccgct
     1321 ggtcgcccgt cagcatgacg atcgcgatgc cggcctcgcg acactgggcg agggcctcct
     1381 tgacgcccgc ccgcaagggg tcggccaggg cgacgaagcc gagaaacgtg aggtcctcga
     1441 tgtgtcggtc ctcgggcgcg gcctcgtccc gccacgcgac ggcaaggacc cggtagccct
     1501 cgttggcgag cgcgcgattg tcgtcgagca gccggcgccg cgtctcgtcg tcgacccggc
     1561 ggagcgtgcc ggcggcatgc acgcgctcac atcgctcgag cacggccccg ggcgccccct
     1621 tgacgaggac ggcatggccg ccttcgggcg tctcgtggaa ggtcgccatg aggcgcgcga
     1681 ccggcgagaa cgggatctcg ccgcggcgcg gccaggccga cgacagggcc gtgacgtcga
     1741 gacctgcctc gagcgccgcg acgagcaggg cggcctccgt cggatcgccg tgcagccgcc
     1801 gtccatcgcc ctcggcctcg acgctcgcgt cgttgacgag cgcggcgacg gtgagcagcc
     1861 gcacgagatc gctgtcctcg agcggggcga tgggtcggcc acgctcgctg aatctgccgt
     1921 gccgggacgt tcccggctcg ccaacctcga cgacacggcc gggcagccat acgcgcgcga
     1981 cggccatgcg gttctcggtc atcgtgcccg tcttgtccga gcagatgacg gtcgtcgcgc
     2041 cgagcgtctc gacggcgggc aggcggcgga cgagcgcgcc ggcgcgggcc agccgccaga
     2101 gaccggcggc cagcgccacg gccgtcacag cgggcagccc ttcgggaatc gccgacacgg
     2161 ccaggctg
//
LOCUS       DATIAZ010000179         2150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1755569, whole genome shotgun sequence.
ACCESSION   DATIAZ010000179 DATIAZ010000000
VERSION     DATIAZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755569"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VLF19_06330"
     CDS             complement(<1..197)
                     /locus_tag="VLF19_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92904.1"
                     /translation="MPTFLAVAVLLGGCAGVAEVTGTATQEDVLQLRADLTTLQTSVR
                     QMKSQVETMGPQADGRLRDRAA"
     gene            complement(224..829)
                     /gene="pal"
                     /locus_tag="VLF19_06335"
     CDS             complement(224..829)
                     /gene="pal"
                     /locus_tag="VLF19_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HSE92905.1"
                     /translation="MSRRWSGMMVSLLVLAVIAAGCAKKPATMQAASAPAPTGAAGRA
                     VEAPAGGPASSTGAQMQPGDAKPAPSGVTAPPVGGARPAPKEFTAVPDLRDVYFDFDK
                     YDIRPADAKTLDSSGAWLKTNGAMLLLIEGHCDERGTNDYNLALGERRAKAAMNYLVS
                     QGVQASRITIISYGEERPVCSEKNEACWAKNRRAHFLVKPR"
     gene            complement(888..>2150)
                     /gene="tolB"
                     /locus_tag="VLF19_06340"
     CDS             complement(888..>2150)
                     /gene="tolB"
                     /locus_tag="VLF19_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HSE92906.1"
                     /translation="AAPPQSSQGVDVWMNIQGAGAKKLNIAIPEFAVVAGPDTTGLAK
                     QLARVAGADLTFSGLFTVVAATGSIPPNDPAALRQSWTDFAAVGAHAGVHGLLTLRRD
                     KVEAEMRLYDLTSPEQRRIAVKTFDMPPDQARRLAHKIADEIVLQFTGERGVADTKMA
                     YVAGPRGAKEIVLADYDGVSSRPVTRNGSINLSPVWSPDGRSLAFLSYRNGYPDLFRA
                     FPYERRPDQTLAAFSGINSSPAWSPDGRSLALTLSKDGNPEVYVLTLATGAFRRLTRH
                     AGIDTEPTWSPSGREIAFISDRVGVPHVYVMDAEGANVRQLTSGGHHTQPRWSPKGDV
                     IVFTTRQGTHDLWAIAPDGSNPRRLTAGPGDNQGATWAPNGRHLAFQSNRLGSWQIFA
                     MLADGSEQTPITRGTTDHTSPSWSSRLP"
BASE COUNT          276 a          748 c          784 g          342 t
ORIGIN      
        1 gccgcccggt cgcggaggcg tccgtcggcc tggggcccca tcgtttcgac ctgggacttc
       61 atctgccgca cggacgtctg cagcgtggtg aggtcggcgc ggagctggag gacgtcctcc
      121 tgcgtggcgg ttccggtgac ctcggcgacg cccgcgcaac caccgagcag cacggcgacg
      181 gcgaggaacg tcggcacgct cctcgccgtc gcgaggaatg cgctcagcgg ggcttgacca
      241 ggaagtgcgc gcgacggttc ttggcccagc acgcttcgtt cttctccgag cagaccggcc
      301 gctcctcgcc gtagctgatg atggtgatcc ggctcgcctg cacgccctgc gagacgaggt
      361 agttcatcgc cgccttggcc cgccgctcgc cgagcgccag gttgtagtcg ttcgtgccgc
      421 gctcgtcgca gtggccctcg atgagcaaca gcatggcgcc gttcgtcttg agccaggccc
      481 cgctgctgtc cagcgtcttc gcgtctgccg gccggatgtc gtacttgtcg aagtcgaagt
      541 agacgtccct gagatccggc accgccgtga actccttcgg agccggccgc gctccgccca
      601 ccggcggagc cgtcacgccg ctcggggcgg gcttcgcgtc gccgggctgc atctgcgcgc
      661 cggtgctcga tgccgggccg cctgctgggg cctccaccgc tctgccggcc gcgcccgtgg
      721 gcgccggcgc cgacgccgcc tgcatcgttg ccggtttctt cgcgcatccc gcggcgatca
      781 cggccaggac cagcaacgac accatcatgc cgctccaacg acggctcacc atgacgcctc
      841 ctctcgcccc gtgcagttgt gccgatctgc cgaacgcctg catcgtatca cggcaggcgc
      901 gacgaccagg aagggcttgt gtggtcggtg gtgccgcggg tgatgggcgt ctgctcggag
      961 ccgtcggcga gcatcgcgaa gatctgccac gacccgagcc ggttgctctg gaacgcgagg
     1021 tgccggccgt tcggcgccca cgtggcgccc tgattgtctc cggggcccgc ggtgaggcgc
     1081 cgcgggttcg agccgtcggg ggcgatggcc cagaggtcgt gcgtcccctg ccgcgtcgtg
     1141 aagacgatca cgtcgccctt cggcgaccag cggggctgcg tgtggtggcc gcccgacgtg
     1201 agctggcgga cgttcgcgcc ctcggcgtcc atgacgtaga cgtgaggcac cccgacacgg
     1261 tcggagatga acgcgatctc gcgtccgctc ggggaccagg tgggctcggt gtcgatgccg
     1321 gcgtggcgcg tgagccgccg gaacgctccg gtggccaggg tgagcacgta gacctcgggg
     1381 ttgccgtcct tcgagagcgt gagcgccagc gaccggccgt cggggctcca ggccggcgag
     1441 gagttgatgc cactgaaggc cgcgagcgtc tggtccggcc ggcgctcgta cgggaaggcg
     1501 cggaagaggt ccgggtagcc gttgcggtac gagaggaacg cgagcgagcg gccgtcgggg
     1561 ctccagaccg gcgacaggtt gatcgagccg ttgcgggtca ccggccggga cgagacgccg
     1621 tcgtaatcgg cgagcacgat ctccttggcc ccgcgcggac ccgccacgta ggccatcttc
     1681 gtgtcggcga cgccgcgctc accggtgaac tgcagcacga tctcgtccgc gatcttgtgc
     1741 gccagccgcc gcgcctggtc cggcggcatg tcgaacgtct tcacggcgat ccggcgctgc
     1801 tcgggggagg tcaggtcgta gagccgcatc tccgcctcga ccttgtcgcg ccggagcgtg
     1861 aggagtccgt gcaccccggc gtgagcgccg accgcggcga agtccgtcca cgattgcctg
     1921 agcgcagcgg ggtcgttggg ggggatcgag cccgtcgccg ccaccaccgt gaagagcccc
     1981 gagaacgtga ggtccgcgcc ggcgacgcgg gcgagctgct tggcgagtcc ggtcgtgtcc
     2041 gggcccgcga cgacggcgaa ctcggggatc gcgatgttga gcttcttggc ccccgcgccc
     2101 tggatgttca tccagacgtc cacgccctgc gaactctgag gcggcgcggc
//
LOCUS       DATIAZ010000180         5342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_835663, whole genome shotgun sequence.
ACCESSION   DATIAZ010000180 DATIAZ010000000
VERSION     DATIAZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835663"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VLF19_06345"
     CDS             <1..262
                     /locus_tag="VLF19_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003781008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSE92907.1"
                     /translation="RLLDRVRVVVGLGRIGWQAYLRARRTLGLGLPRPMPRFAHGAVA
                     TFEDGVSLIASYHPSQQNTFTGKLTRPMLRSVLQRARRLAEA"
     gene            complement(414..1481)
                     /locus_tag="VLF19_06350"
     CDS             complement(414..1481)
                     /locus_tag="VLF19_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSE92908.1"
                     /translation="MLIWGRPARTLAFLVFVTAGLRSPAAAQPCTDPLTAVFERLSPA
                     VVSIQATKINKAKPQRRFETVVGSGVIVERDGQVVTNAHVVDGAASLSVTLDSGTHLP
                     ARVLGLDPVLDIALLRLDTRGPLPAARLGDSASVRVGDEVVAIGSPIGLEQTMTRGIV
                     SGLNRLLPGLPEQPMIQTDAAINPGNSGGPLVTRCGTVVGINTFISEDAQSIGFAVPV
                     NAVKAVLRDLRENGRVIRPWLGIQGRTVDARLTGLVRMPLTPGYLAEVVYDGSPAERA
                     GIQGGSVSVVIQGEEYLLGGDIVTAIGGHPVRNHEDYLARVNALRPGQKVRVSVFREG
                     HTREVTLGVVERPRLPSDLAE"
     gene            1543..2880
                     /locus_tag="VLF19_06355"
     CDS             1543..2880
                     /locus_tag="VLF19_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSE92909.1"
                     /translation="MRRLTISTALLLLLLAPWVSPTGGAETPRPVTAGVLAATLDNGL
                     RVLLQEDHRSPIVSVQVWYRVGSRNELRGATGLAHLLEHMMFKGTPTHGPREFARLVE
                     QNGGQDNAFTSQDVTSYFVNIAADRIDMVLGLEADRMRNLLLDTKDVDAEREVVMEER
                     RTRTEDDPEGFLGEEVSSVAFKAHPYGSPIIGWTEDLKRLTADELRAFYRTYYVPNNA
                     ILVAVGDFKAPETLAKIERLFGALPRGADPPPVLAVEPPQNGERRVLVRKQAQLPIVY
                     LAWHVPNHTSPDAAALEVLSTVLSGGRASRLYRRLVYERQLALEAGGDYPYFSLDPNL
                     FWFWATAMPGQTPETLEQALLEEMERLRSEPATVEELDRAKNQIEAAFVFQQDSVYRR
                     ASLLARFETLGGFERLDGFVPAVRAVTVADLQRVARAYFPVDRKSLGVLLPTP"
     gene            2907..4280
                     /locus_tag="VLF19_06360"
     CDS             2907..4280
                     /locus_tag="VLF19_06360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSE92910.1"
                     /translation="MGRRRRLAAMARRGALVAAFVVAAIVMAAAGPAWQRPAPASAAR
                     AALAHREILPNGLVLLVAERPTVPIVVVRVWQRAGAVFDPDDRAGLANLTGAVLTRGA
                     GQRTAKQIDEAIEFVGGSLEAAAGRDGLTVSLSVLARDLGLGLDLLRDVVLAPTFPDD
                     EVNRKVAEIQAAIKRAEQNPETVAARVLAGLVFPSHPYGRPVEGTRESVSRLTRDDVI
                     RFYRDHARPDTTIVAVVGAVTVDEARRAVTARFGGWTRPPRPAADVPPAAPPASAGDE
                     TVIRELTQATIMLGRQAVNQLDPDYYPLAVASYVLGGGSASRLYGRVRDQNGLAYSVF
                     AWLAPGRHGASFNVSAQTRNREVTRATDILRDELARMVREPVPEGELKLAKDYLIGSF
                     PLRLDTSAKVADFIVAIESSGLGLDYPDRYRERIARVTAGDVQRVAARFLSPATFSRV
                     VVAGKAP"
     gene            4277..4849
                     /locus_tag="VLF19_06365"
     CDS             4277..4849
                     /locus_tag="VLF19_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92911.1"
                     /translation="MTRVRLAVLGLLLAVSSEAAGAASLTLGDPARQEALRVGQRSVT
                     SETFGTEWRVVNGGGESVSVMTPFHRLALAARHSAFKNEPLNSRDQDRLVNDLRDRLM
                     LWVELHGDKEDFARFLAPRLSVGDRIIQPSIVQNERTAIRGEGGRYLARCVYWFPVKD
                     VDGDSRVTLLIQTPEGQERTRFEIDLAKMR"
     gene            4876..>5342
                     /locus_tag="VLF19_06370"
     CDS             4876..>5342
                     /locus_tag="VLF19_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92912.1"
                     /translation="MARHPTPPAPRLVPAHGLAGIGIIVAAEVLLFGGNRLVGEWFTP
                     VVWTGYVLLLDAVVARLTGRSYLTTGRLELLLVALASIAAWWLFELYNAPRFWRGGDA
                     ARGLWWQYHRLEPDPFLRRVGYDWAFATILPALFLTAAALRATVFARVRVRPWA"
BASE COUNT          702 a         1965 c         1900 g          775 t
ORIGIN      
        1 gcggctgctc gaccgcgtga gggtcgtcgt cgggctcggc cgcatcggct ggcaggcata
       61 cctgcgggcg cggcggaccc tcggtctcgg gctcccccgt ccaatgccgc gcttcgctca
      121 cggcgccgtc gccacgttcg aggacggcgt gtcgctgatc gcgtcgtacc atccgagcca
      181 gcagaacacg ttcaccggca agctcacgcg ccccatgctg cgctcggtcc ttcagcgtgc
      241 gcggcgcctg gccgaggcgt gacaccgacg cacgagacgc ggacggccat ccccgcggga
      301 gcgctccggc aggaccgcag cacgggccga gcagccccgc gccgcgaggc gagtggtgac
      361 atggcctggg ttcgggcgag cgtccggctg cccatgccgt gctcggtgct cggctactcc
      421 gccaggtcgg agggcaggcg cggacgctcg accacgccga gcgtgacctc gcgcgtgtgc
      481 ccctcgcgga acacgctcac ccggaccttc tggcccggcc tgagcgcgtt cacgcgggcg
      541 aggtaatcct cgtgattgcg cacggggtgc ccgccgatgg cggtgacgat gtcgccgccg
      601 agcaggtact cctcgccctg gatgacgacc gacacgctgc cgccctggat ccccgcgcgc
      661 tccgccgggc tgccgtcgta gacgacctcg gcgagatacc ccggcgtcag cggcatgcgc
      721 acgaggccgg tcagacgagc gtccaccgtc cgcccctgga tgccgagcca ggggcggatg
      781 acgcgcccgt tctctcggag gtcgcgcagc accgccttga ccgcgttcac gggcacggcg
      841 aagccgatgc tctgcgcgtc ctcggagatg aacgtgttga tgccgacgac ggttccgcag
      901 cgcgtcacga gaggcccgcc ggagttgccc ggattgatgg cggcgtcggt ctggatcatc
      961 ggctgctcgg gcaggccggg cagcaggcgg ttcagtccgc tcacgatccc gcgcgtcatc
     1021 gtctgctcga gcccgatggg gctgccgatc gccaccacct cgtcccccac tcgcaccgac
     1081 gcggagtcgc cgaggcgtgc ggccggcagc ggcccgcgcg tgtcgaggcg gaggagtgcg
     1141 atgtccagca cggggtcgag tccgagcacc cgtgcgggca ggtgcgtgcc ggagtcgagc
     1201 gtcaccgaca gcgacgcggc gccgtcgacg acgtgggcgt tcgtcaccac ctgcccgtcg
     1261 cgctcgacga tgacgcccga gccgacgacg gtctcgaagc gccgctgggg cttcgccttg
     1321 ttgatcttgg tcgcctggat cgacacgacg gccggcgaca gccgctcgaa gaccgcggtg
     1381 agcgggtcgg tgcagggctg cgcggccgcg ggtgagcgga gaccggcggt gacgaagaca
     1441 aggaacgcca gcgttcgcgc cggtcgcccc cagatgagca tcagcgacga tcatacaccc
     1501 ggcgcccggg cgcgacgcag acgcggcgct ataatcggcc tcgtgcgcag actcacgatc
     1561 tcgacggccc tgctgctcct gctgctggcg ccgtgggtgt ctcccaccgg cggcgccgag
     1621 acgccgcggc ctgtcaccgc gggcgtgctg gcggcgacgc tggacaacgg cctccgggtg
     1681 ttgctccagg aggaccaccg gagccccatc gtgtcggtcc aggtctggta ccgcgtcggc
     1741 tcgcgcaacg agctgcgcgg cgccaccggc cttgcccacc tcctcgagca catgatgttc
     1801 aagggcacgc cgacccacgg ccctcgcgag ttcgcgcgcc tcgtcgagca gaacggcggc
     1861 caggacaacg ccttcacctc gcaggacgtc acctcgtact tcgtcaacat cgccgccgac
     1921 aggatcgaca tggtgctcgg tctcgaggcg gaccgcatgc gcaacctcct gctcgacacg
     1981 aaggacgtcg acgccgagcg agaagtcgtc atggaggagc ggcgcacgcg cacggaggac
     2041 gatcccgagg ggttcctcgg cgaggaggtc tcgtccgtcg ccttcaaggc gcacccgtac
     2101 ggctcgccga tcatcggctg gacggaagac ctcaagcggc tcaccgccga cgagctccgc
     2161 gccttctacc gcacgtacta cgtcccgaac aacgcgatcc tggtcgccgt cggcgacttc
     2221 aaggcgcccg agacgctcgc gaagatcgag cgcctcttcg gtgcgctccc gcgcggggcc
     2281 gacccgccgc ccgtgctcgc cgtcgagccg ccgcagaacg gcgaacgccg ggtgctggta
     2341 cgcaagcagg cgcagttgcc gatcgtgtat ctcgcctggc acgtgccgaa ccacacgtcg
     2401 cccgacgccg ccgccctcga ggtgctgtcc accgtgctct ccggaggccg cgcctcgcgg
     2461 ctctaccgtc gtctggtgta cgagcggcag ctcgcgctcg aggccggcgg cgactatccc
     2521 tacttctcgc tcgaccccaa cctcttctgg ttctgggcga ccgcgatgcc cgggcagaca
     2581 cccgagaccc tcgagcaggc gctgctggag gagatggagc ggctgcgcag cgagccggcg
     2641 acggtggagg agctggaccg cgccaagaac cagatcgagg ccgcgttcgt cttccagcag
     2701 gactccgtgt accgccgggc ctcgctgctc gcccgtttcg aaaccctcgg cggcttcgag
     2761 cgcctcgacg gcttcgttcc cgccgtacgg gcggtgacgg tggccgacct ccagcgcgtg
     2821 gcgcgcgcgt acttccccgt cgaccggaag agcctcggtg ttctgctccc cacaccgtga
     2881 tggcccccac gcggtctcgt gaatccgtgg gccgccggcg ccgtctcgcg gccatggcgc
     2941 gccggggcgc ccttgtcgcc gccttcgtcg tcgcggcgat cgtcatggct gccgccgggc
     3001 cggcctggca gcggccagcg ccggcctccg ccgcgagggc ggcgctggcc catcgcgaga
     3061 tcctgcccaa cgggctcgtg ctgctcgtgg ccgagcgccc gacggtgccg atcgtcgtcg
     3121 tccgggtgtg gcagcgggcg ggcgccgtgt tcgacccgga cgaccgggcc ggtctggcca
     3181 acctcacggg cgccgtgctc acgcggggcg cgggccagcg cacggccaaa cagatcgacg
     3241 aggcgatcga gttcgtgggc gggagcctgg aggcggccgc cgggcgcgac ggactgaccg
     3301 tctcgctgtc cgtgctcgcc cgcgacctcg gcctcggcct cgacctcctg cgggacgtcg
     3361 tgctcgcccc gaccttcccg gacgacgagg tgaaccgcaa ggtggccgag atccaggccg
     3421 ccatcaagcg cgccgagcag aacccggaga cggtggcggc gcgcgtgctg gccgggctcg
     3481 tgttcccgag ccatccgtac ggccggccgg tcgagggtac gcgcgagtcc gtctcccggc
     3541 tcacccgcga cgacgtgatc cgcttctacc gcgaccacgc gcgccccgac acgacgatcg
     3601 tggccgtggt tggcgcggtc acggtggacg aggcgcgccg cgccgtcacc gcgcgcttcg
     3661 gcggctggac gcgtccgccg cggccagccg ccgatgtgcc gccggccgcg ccgccggcct
     3721 ccgccggcga cgagacggtc atccgggagc tgacccaggc gaccatcatg ctcgggcgcc
     3781 aggctgtgaa tcagctcgat cccgactact acccactcgc cgtggcctcg tacgtgctgg
     3841 gtggcggctc ggcctcgcga ctgtacgggc gcgtgcgcga ccagaacggg ctcgcgtact
     3901 cggtcttcgc ctggctcgcc cccgggcggc acggcgcgtc cttcaacgtc tcggcgcaga
     3961 cgcgcaaccg ggaggtgacc agggccaccg acatcctgcg cgacgagctg gcccggatgg
     4021 tgcgcgagcc ggtgcccgag ggtgagctga agctcgccaa ggattatctc atcggcagct
     4081 tcccgctgcg tctggacacg tcggcgaagg tcgccgactt catcgtcgcc atcgagtcct
     4141 cggggctcgg cctcgattac cccgaccgct atcgcgagcg catcgcgcgc gtgacggcgg
     4201 gggacgtgca gcgcgtggcc gcccggttcc tctcgcccgc cacgttctcc cgggtggtcg
     4261 tcgccggcaa ggcgccgtga cacgcgtgcg gctcgccgtg ctcggcctgc tgctcgccgt
     4321 ctcgagcgag gccgcgggcg cggcctcgct gacgctcggg gatccggcgc gccaggaggc
     4381 gctccgggtc gggcagcgat cggtcacgag cgagaccttc ggcacggagt ggcgggtcgt
     4441 gaacggcggg ggggagagcg tgagcgtcat gacgccgttc caccgcctcg cgctggcggc
     4501 gcggcactcc gccttcaaga acgagccgct gaactccagg gatcaggacc gcctggtgaa
     4561 cgatctccgc gaccgcctga tgctgtgggt ggagctgcac ggcgacaagg aggacttcgc
     4621 ccggttcctg gcgccgaggc tgtccgtcgg cgaccgcatc atccagccct cgatcgtcca
     4681 gaacgagcgg acggcgatcc ggggcgaggg cgggcgctac ctggcgcgct gcgtgtactg
     4741 gttccccgtc aaggacgtgg acggcgactc ccgcgtgacc ctgctgatcc agacgcccga
     4801 gggacaggag cgcacccgct tcgagatcga cctcgccaag atgcggtagc cgcggacggg
     4861 cacgggagca ggcccttggc tcgacatccc actcccccgg cgcctcgtct cgtacccgcc
     4921 cacggcctcg ccgggatcgg gatcatcgtt gccgcggagg tgctcctgtt cggcgggaac
     4981 cggctcgtcg gcgagtggtt cacgcccgtc gtgtggacag gctacgtgct gctgctcgac
     5041 gccgtcgtcg cccggctcac cggccgctcg tacctgacga cggggcggct ggagctgctg
     5101 ctggtggcgc tcgcctcgat cgccgcctgg tggctcttcg agctgtacaa cgcgccacgg
     5161 ttctggcgcg gcggcgacgc cgcgcgcggc ctctggtggc aatatcaccg gctggagccg
     5221 gaccccttcc tgcgccgcgt gggctacgac tgggcgttcg cgacgatcct cccggcgctg
     5281 ttcctcaccg ccgcggcgct ccgtgcgacg gtcttcgcgc gggtacgcgt gcggccgtgg
     5341 gc
//
LOCUS       DATIAZ010000181        11891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1229641, whole genome shotgun sequence.
ACCESSION   DATIAZ010000181 DATIAZ010000000
VERSION     DATIAZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11891
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229641"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /gene="trpC"
                     /locus_tag="VLF19_06375"
     CDS             <1..640
                     /gene="trpC"
                     /locus_tag="VLF19_06375"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HSE92913.1"
                     /translation="GGVRLVAEVKKASPSRGVLAPSLDPVELAARYARHGADAISVLT
                     DEKYFQGHLDLLAPIRVAVSVPLLRKDFTLDEYHLWEGRAAGADAALLIVAILEPPLL
                     RDLVQAAKGIGLAALVECHTAAEIETALAAGARIVGINNRDLRTFETRIETTIDLVPQ
                     IPPGPIVVSESGFFTGEQVRRVAAAGAHAVLVGEGLVRAEDVAAKIAELKGA"
     gene            637..1302
                     /locus_tag="VLF19_06380"
     CDS             637..1302
                     /locus_tag="VLF19_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HSE92914.1"
                     /translation="MIRVKICGITNAEDAMTAVEASADALGFIFVENTPRYVTPEQVA
                     AIIGRLPPFVTPVGVFWDHPRGHVKAVAETCGLRALQFHGDETPEDVAGYDLPVIKTV
                     KLPPVSTIEGLREYRVGDWAQVLRYSPVSSAVLLDTAARWSEGEAREPLEWQTVRWLQ
                     SGTGRRFRLILSGGLTPDNVARAVIVAQPYAVDVNSGVEARPGKKDPDKVRRFVAEAR
                     RAG"
     gene            1299..2498
                     /gene="trpB"
                     /locus_tag="VLF19_06385"
     CDS             1299..2498
                     /gene="trpB"
                     /locus_tag="VLF19_06385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSE92915.1"
                     /translation="MRRLPDARGHFGPYGGRFVPETLMAPLQELERAYASARRERRFR
                     TELASLLRDYAGRPTPLYFARHLSEHVGGARIWLKREDLCHTGAHKINNVLGQGLLAA
                     RMKKGRLVAETGAGQHGVATATAAALLGLECEVYMGVEDVERQALNVFRMRLLGARVV
                     PVESGSRTLKDAINEALRDWVTNVRTTYYLLGSALGPHPYPMMVRDFHTVIGKETRMQ
                     ARRQIGRLPDVLVACVGGGSNAIGLFHPFVDDRRVKIVGVEAGGHGVATGRHGASLSA
                     GSVGVLHGCMTYLLQNDEGQIATAHSISAGLDYPGVGPEHAYYKDAGRFEFVSVTDEE
                     ALEGFQALARLEGIMPALESSHAVAYAMRLAKRLPRARVIVVGLSGRGDKDVHVVARA
                     LGQEPPE"
     gene            2495..3340
                     /gene="trpA"
                     /locus_tag="VLF19_06390"
     CDS             2495..3340
                     /gene="trpA"
                     /locus_tag="VLF19_06390"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSE92916.1"
                     /translation="MTRITATFERLRAAGERALVVYVTAGDPSLDVTARLVVEADRRG
                     ADVIELGVPFSDPLADGPVIQRAGQRALAAGTSLVRVLESVAKIRVDVRAPIVLFTYY
                     NPVLVFGLAAFAGAAARAGVDGVIVVDLPPEEADPLDAELRRTGIDLIHLAAPTSTSE
                     RLRLVARRSRGFVYLVSLTGVTGERTALPPDLEAQVRTLRLVTTKPICVGFGIGRPEQ
                     VAAVGAVADGAIVGSAIVRLVEERAGSPTLVSDVGDFIAALKQPLRAVGPGVPAAGGR
                     GSADP"
     gene            3542..4399
                     /gene="accD"
                     /locus_tag="VLF19_06395"
     CDS             3542..4399
                     /gene="accD"
                     /locus_tag="VLF19_06395"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSE92917.1"
                     /translation="MAWFRRKDDGAEPKGRKVVMAEGLWIKCDSCKEIVYRAEVERAG
                     RVCPKCRYAFRMGARERIAQLADAGTFEEHDAALTAADPLGFRDSKRYRDRVRAAREK
                     TGSREAVITGLARIGGLPAVLCAFDFAFLGGSMGSAVGEKLTRAIETAIAKHVPLVIV
                     SSSGGARMQEGILSLMQMAKTAAALERLGTHHLPYVSILTDPTTGGVTASFAMLGDVV
                     LAEPRALIGFAGPRVIAETIRQPLPEGFQRSEFLLQHGQLDLIVERRELKDTLRRLLA
                     FFADQPPSG"
     gene            4396..5727
                     /locus_tag="VLF19_06400"
     CDS             4396..5727
                     /locus_tag="VLF19_06400"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSE92918.1"
                     /translation="MTYAEAVARLTALRGGEHAGIRPGLERIEGLLAALGHPESRYAI
                     VQVGGTNGKGSVAAMLATILKAAGRRVGLYTSPHLVSFRERIRVDAEAIGEDAVVDGV
                     EALGTLVARFDATMFEATTALALDHFAREAVDVAVLEVGLGGRLDATTVGRPVAVILS
                     RIDLDHEAVLGSTVEAIAVDKACIVRAGVAYSAAQAPEAARVIVARAEATGVPLLMEG
                     RDLSVRVEFRDLGGQRIACEGPGFSLTSLHLPLLGTYQPGNALLAVAAAHGLGAGEAA
                     VRAGLARARWPGRFQVVRGTPTLVLDAAHNPAGARALADSLRAYFPDRPLTLVLGVSR
                     DKDAAAMLAALAPLARRLILTRAATARAAEPETLRALLPAGGAGAEAVSSVAEALDLA
                     TRSAETPVVCVAGSLFVVGEALTHLGGTEAACLIENGADSMGSLEGARDTS"
     gene            5724..7988
                     /gene="lptD"
                     /locus_tag="VLF19_06405"
     CDS             5724..7988
                     /gene="lptD"
                     /locus_tag="VLF19_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HSE92919.1"
                     /translation="MTLVRRAVLLLAGLVAAGAAAVPMSAVAAQAPTSVTVTTPSGDV
                     RLIADRLEEIGADDLVIATGNVEVTRGTQRLTADRVELNRATGDVVAEGRAVFYDGDD
                     RLFGERIEYNYRTGTGVVHDGAARAAPYYRIAGERMERLGEGRYHVRRGVFTTCEDDP
                     PTWSFRFGSADADMESYLYGTEASFWVKRFPLIPFVPFFAAAIRRERQTGFLFPRFGS
                     SSRKGTYLELPFFWAISDSQDALITLDAYGDIGLGANLEYRYLLSRTHYGSVNGFYVN
                     EFERSDDLQDGVDDNRGWWSIEHRWRIGPRFDLTVDVNGVSDDRLFRDYGDPLATRAS
                     QRVESNIFLTRSWPTANLAGNLFFYRDLTTPEAVELHRLPDIRYAAPRQPLPWLPAAS
                     RLLGELNAQYVNFVRDVGSEGQRLDVQPILSRPFSLGGYVTLTPFVGGRFTAYDKTVT
                     GTRAVSSGAVTVETTDDEPRLRRLVQLGGDVEARASRVYEVGGFGGIDAVLHSIEPRV
                     NYTFIEGSDLLRQTGPDTFRPNRLPQWDAVDAISETSLITYSLTNRVRGRTVAPAGTE
                     PLRWEAVRVALGQTYNLHDDDRPFGDVLGILIVNPGQILGFRSDASYNVYGNGLTTAT
                     ADVTVEVPRFQAAVGARVARDREVDLSGRVQTTTRQSFLQTTMRAELFPWAVVRLESN
                     WDVKQNVFVENRYGVDLRWQCWAFTLEYVRRHNDEDEVRFALNLLGVGQPLQFGSRLQ
                     TSGGLNTSGDGRIR"
     gene            7985..9196
                     /locus_tag="VLF19_06410"
     CDS             7985..9196
                     /locus_tag="VLF19_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSE92920.1"
                     /translation="MIPHIDLGRQHAGLRDELLAAMARVLDSSRFVLGGEGEALEREL
                     AALTGVRHAIGVASGTDALRLSLAALGVGPGDEVITSAFSFVASASTIVMTGATPVFV
                     DIDPETYALDVAAAERAVTSRTRAIVPVHLYGHPAAMDAVAALARGHGLVVIEDAAQA
                     IGAAWQGRAVGTWGDTACLSFYPTKNLGACGDAGMVLTGRDDLAERLRRLRHHGDGGR
                     YRHVELGYASRLDELQAAILRVKLRHLQDWTEAKRRLAARYGRLLAGLPLGLPVERPG
                     ARHVYHLYTVRHQRRDALAKALADLGVGTAVHYPIPVPDQPMFAGAHAAGRTGGRDEQ
                     RRGASRAAETSGSRRRSASEASPTESGERRWPEAWRAAREVLALPCFAELTDDEQDRV
                     AAAVRSALQGT"
     gene            9251..10117
                     /locus_tag="VLF19_06415"
     CDS             9251..10117
                     /locus_tag="VLF19_06415"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HSE92921.1"
                     /translation="MLTAVWLSLRPRQWVKNLFVFAGLIFGHQLLTPAIGPALATFAI
                     FCALSGAVYLVNDVADRDKDRQHPLKRRRPIASGRLPASVAVAVAVVLAVAGVLASAA
                     IERRLAAVAVAYAALLVAYSLWLKHLVIVDVIVVAVGFVLRAVAGAVAVDVEISGWLL
                     ICTVLIALFLALGKRRHEYLTLGAEAAGHRPILAEYSAGLLDQMIAVVTASTLTAYAL
                     YTMSPETIAKFGTRLLPATLPFVLYGIFRYLYLLYRKQMGGSPSDLFLSDRPLLVNTL
                     LWMLAVVLIIYW"
     gene            10311..11390
                     /locus_tag="VLF19_06420"
     CDS             10311..11390
                     /locus_tag="VLF19_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSE92922.1"
                     /translation="MTRSQVAVLRTRPATVVEDYGRLMRLVKYDQVLSRDTDLIVKLN
                     LSWTKYFPACSSQPWQVDGILATLLADGYDRARILPVENRTVVTDPVQGCRNNKWEPV
                     LRRHGLPFVPLTDVEWTVYPFKTPLLKLNDIFPEGIQIPKMYIGKNVCHAPTTKTHGH
                     TTTTGAIKNAFGGLLKEVRHYAHEFIHEVMVDLMLMQRELHPGVIAVMDGTVMGDGAG
                     PRTMVPRVGNLILASADQVAIDAIAAKVMGFDPLTIPYLRMCHERGLGVADPREIEIV
                     GDPDAAEVSMGFTTSRSLVIWGDQLIRRGPLRPLKRLLLHSPLVVWAPLASNVYHDWL
                     WYPTVGRSRIRAFAGTPWGRHFETY"
     gene            11548..>11891
                     /locus_tag="VLF19_06425"
     CDS             11548..>11891
                     /locus_tag="VLF19_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HSE92923.1"
                     /translation="MTAASPETVRVRFAPSPTGHLHVGGARTALFNWLYARHHRGAFI
                     LRIEDTDRSRSTDENIGAIVEALEWLGLDWDEGPPAPGYRQTERLELYRAHAARLLAA
                     GRAYYCDCPPEAL"
BASE COUNT         1573 a         4371 c         4241 g         1706 t
ORIGIN      
        1 ggggggcgtc cggctcgtcg ccgaggtgaa gaaggcgtcg ccctcgcggg gcgtgctggc
       61 tccctccctg gacccggtcg agctggcggc gcgctacgcc cgccacggcg ccgacgcgat
      121 ctcggtgctc accgacgaga agtacttcca gggccatctc gacctcctgg cgccgatccg
      181 cgtcgccgtt tccgtgccgc tgctgcgcaa ggacttcacg ctggacgagt accacctgtg
      241 ggagggacgc gccgcgggcg ccgatgccgc gctgctcatc gtggcgatcc tcgagccccc
      301 gctcctccgg gacctcgtcc aggccgcgaa gggcatcggc cttgccgcgc tcgtggagtg
      361 ccacacggcg gccgagatcg agaccgcgct cgccgccgga gcccgcatcg tcggcatcaa
      421 caaccgcgac ctgcgcacct tcgagacgcg catcgagacg acgatcgatc tcgtgccgca
      481 gattccaccg gggccgatcg tggtcagcga gagcggcttc ttcacgggcg agcaggtgcg
      541 ccgggtcgcc gccgcgggcg cccatgcggt gctggtgggc gaggggctgg tgcgcgcgga
      601 ggacgtcgcg gcgaagatcg cggaactcaa gggcgcatga tccgcgtcaa gatctgcggc
      661 atcaccaatg ccgaggacgc gatgacggcc gtcgaggcca gcgcggacgc cctcgggttc
      721 atcttcgtcg agaacacgcc tcgctacgtc acgccggagc aggtggcggc gatcatcggg
      781 cggcttccgc ccttcgtgac gccggtcggc gtcttctggg atcatcctcg gggtcacgtc
      841 aaggcggtgg cggagacgtg cgggctccgc gcgctgcagt tccacggcga cgagacgccg
      901 gaggacgtgg ccggctacga cctgcccgtg atcaagaccg tcaagctccc gccggtctcg
      961 acgatcgagg gactccgcga gtaccgcgtg ggcgactggg cgcaggtgct acggtacagc
     1021 ccggtctcct ccgccgtgct cctggatacc gccgcccggt ggagcgaggg cgaggcgcgc
     1081 gagccgctcg agtggcagac ggtgaggtgg ctgcagagcg ggaccggccg tcgcttccgg
     1141 ctgatcctgt ccggcgggct gacccccgac aacgtggcgc gagccgtgat cgtcgcgcag
     1201 ccgtacgcgg tcgacgtgaa ttccggcgtg gaggcccggc ccgggaagaa ggaccccgac
     1261 aaggtccgcc ggttcgtggc cgaggcccgg cgcgcgggat gaggcggctg cccgacgccc
     1321 gcggacactt cggcccctac ggcggccgtt tcgtgcccga gaccctcatg gcgccgctcc
     1381 aggaactcga gcgcgcctat gcgagcgccc ggcgcgagcg ccgcttccgg accgagctgg
     1441 cgtcgctgct gcgcgactac gcggggcgcc cgacaccgct ctacttcgcc cggcatctgt
     1501 ccgagcacgt cggcggcgcg cgcatctggc tcaagcgcga ggacctctgc cacacgggcg
     1561 cccacaagat caacaacgtg ctcgggcagg ggttgctcgc ggcgcgcatg aagaagggcc
     1621 ggctcgtcgc cgagaccggc gccggccagc acggcgtggc cacggcgacg gccgccgcgc
     1681 tgctcgggct cgagtgcgag gtctacatgg gggtcgagga cgtcgagcgc caggcgctca
     1741 acgtcttccg catgcgcctg ctcggcgccc gcgtggtccc ggtggagagc ggcagccgca
     1801 cgctgaagga cgccatcaac gaggcgctgc gcgactgggt gacgaacgtg cgcacgacgt
     1861 actacctgct cggctccgcg ctggggccgc acccctaccc gatgatggtg cgcgacttcc
     1921 acacggtgat cggcaaggag acgcggatgc aggcgcggcg gcagatcggt cgcctccccg
     1981 acgtgctggt ggcctgcgtc ggcggcggct ccaacgccat cggcctgttt catcccttcg
     2041 tggacgaccg gcgcgtgaag atcgtggggg tcgaggccgg cggacacggc gtggcgacgg
     2101 gacggcacgg cgccagcctg agcgcgggct cggtcggggt gctccacggc tgcatgacct
     2161 acctgctgca gaacgacgag ggccagatcg cgacggcgca ctcgatctcc gcggggctcg
     2221 actaccccgg cgtcggcccc gagcacgcct attacaagga cgccggccgc ttcgagttcg
     2281 tctcggtgac cgacgaggag gcgctcgagg ggttccaggc gctggcgcgg ctggagggca
     2341 tcatgccggc gctggagtcc tcccacgccg tcgcctacgc gatgcggctc gccaagcgcc
     2401 tgccccgggc cagggtcatc gtcgtcggcc tctcggggcg cggcgacaag gacgtgcacg
     2461 tggtcgcgcg cgcgctgggc caggagccac ccgagtgacg cggatcacgg cgacgttcga
     2521 gcgcctgcgt gcggccggcg agcgggccct cgtcgtctac gtcaccgcgg gcgacccgtc
     2581 gctcgacgtg accgcccgcc tcgtcgtcga ggccgatcgc cggggcgcgg acgtgatcga
     2641 gctgggcgtg ccgttctcgg acccactggc cgacggcccg gtgatccagc gcgccgggca
     2701 gcgcgcgctc gccgcgggga cctcgctcgt gcgggtcctg gagtcggtcg cgaagatccg
     2761 cgtggacgtc cgggcgccca tcgtgctgtt cacgtactac aatcccgtgc tggtcttcgg
     2821 gctcgccgcc ttcgccgggg ccgccgcccg cgcgggcgtc gacggcgtga tcgtcgtcga
     2881 cctgccgccc gaggaggccg acccgctcga cgcggagctg cgccggacgg gcatcgacct
     2941 gatccacctg gccgcgccga cgtccacgag cgagcgcctg cgactcgtgg cccggcgaag
     3001 ccggggcttc gtctatctgg tgtcgctcac gggcgtgacg ggcgagcgca cggcgctgcc
     3061 gcccgacctc gaggcccagg tgcgcacgct ccggctcgtc accacgaagc cgatctgcgt
     3121 gggcttcggc atcggccggc cggagcaggt ggcggcggtg ggtgccgtcg ccgacggcgc
     3181 catcgtgggc tcggcgatcg tgcggctggt cgaggagcgc gccggctcgc cgacgctcgt
     3241 ctccgacgtc ggcgacttca tcgccgcgct caagcagccc ctgcgcgccg tcgggccggg
     3301 cgtgccggcc gcgggtggca gggggtccgc cgacccctga tgcgccgcgc ggcgtctgtc
     3361 gtcacgtggg tccatcggcg cgctacgcgt gtgacggacg gcggggaaca ggtcggcggg
     3421 ccccctgcca cccgcggccg gcacgcccgg cccgacgccc cgcgaccgcc gctcggcggt
     3481 cgcccggccc cggcgaatca ggacgagcgg cgcgaggccc tcgcgggctt aggatagcgg
     3541 catggcctgg ttcaggcgga aggacgacgg cgccgagccg aagggtcgca aggtggtcat
     3601 ggccgagggc ctgtggatca agtgcgactc ctgcaaggag atcgtctacc gcgccgaggt
     3661 cgagcgcgcg gggcgcgtgt gcccgaagtg ccgctacgcc ttccgcatgg gggcgcgcga
     3721 gcgcatcgcg cagctcgccg acgccggcac gttcgaggag cacgacgccg cgctgaccgc
     3781 cgcggatcct ctcggctttc gggacagcaa gcgctaccgc gaccgcgtgc gcgcggcgcg
     3841 cgagaagacg ggcagccgcg aggcggtgat cacggggctc gcgcggatcg gtggcctgcc
     3901 cgcggtcctg tgcgccttcg acttcgcgtt cctcggcggt agcatgggct cggcggtcgg
     3961 cgagaagctc acgcgcgcca tcgagacggc catcgccaag cacgtgcccc tggtcatcgt
     4021 gtcctcctcc ggcggggccc gcatgcagga aggcatcctc tcgctgatgc agatggcgaa
     4081 gacggcggcc gcgctcgagc ggctcggcac ccaccacctg ccctacgtct cgatcctgac
     4141 cgacccgacg accggcggcg tcaccgcgag cttcgccatg ctcggcgacg tcgtcctcgc
     4201 cgagccgcgc gcgctgatcg gcttcgccgg cccgcgcgtg atcgccgaga cgattcgcca
     4261 gcccctgccg gaggggttcc agcgctccga gttcctcctc cagcacggcc agctcgacct
     4321 catcgtggag cgccgcgagc tgaaggacac gctccgacgc ctcctcgcct tcttcgcgga
     4381 ccagccgccg tccggatgac ctacgccgag gccgtcgccc gcctcaccgc cctgcggggc
     4441 ggcgagcacg cgggcatccg gccggggctc gagcgcatcg aggggctgct cgccgcgctc
     4501 ggccaccccg agagccgcta cgcgatcgtg caggtgggcg gcaccaacgg caagggctcg
     4561 gtcgccgcca tgctcgcgac catcctcaag gccgcgggcc ggcgcgtcgg cctctacacg
     4621 tctccgcacc tggtctcgtt ccgcgagcgg atccgagtcg acgccgaggc gatcggcgag
     4681 gacgccgtcg tcgacggcgt cgaggcgctc ggcacgctgg tggcgcgctt cgacgccacg
     4741 atgttcgagg cgacgacggc cctggccctc gaccacttcg cccgcgaggc cgtggatgtc
     4801 gccgtgctcg aggtgggcct cggaggccgc ctcgatgcga ccacggtggg gcgcccggtg
     4861 gccgtcatcc tgagccgcat cgacctcgac cacgaagcgg tgctgggatc cacggtggag
     4921 gccatcgccg tggacaaggc ctgtatcgtc cgcgccggcg tcgcctacag cgcggcgcag
     4981 gcgccggagg ccgcgcgcgt catcgtcgcg cgcgccgagg ccacgggcgt gccgctgctg
     5041 atggagggcc gcgacctctc cgtgcgggtc gagttccgcg atctcggcgg ccagcggatc
     5101 gcctgcgagg gccccggctt ctccctgacg agcctgcacc tgccgctcct ggggacctat
     5161 cagcccggca atgccctgct cgccgtggcg gccgctcacg gcctcggcgc gggggaggct
     5221 gcggtccgcg cgggcctggc gcgcgcgcgc tggccgggac gcttccaggt cgtccgcgga
     5281 acgccaacgc tcgtgctcga cgccgcgcac aatcccgccg gagcgcgggc gctcgcggac
     5341 tcgctgcgcg cgtacttccc cgatcggccg ctcaccctcg tgctcggtgt ctcgcgcgac
     5401 aaggacgcgg cggcgatgct cgcggcgctg gcgccgctgg cgcgccgtct catcctcacc
     5461 cgcgcggcga cggcgcgggc ggccgagccg gagacgctgc gcgcgctcct gccggctggc
     5521 ggcgccgggg cggaggccgt gtcctcggtg gccgaggccc tggacctggc cacgcggtcg
     5581 gcggagaccc ccgtcgtctg tgtggcgggc tcgttgttcg tcgtcggcga ggcgctcacg
     5641 cacctcggcg ggacggaggc agcttgtctc atcgaaaacg gggctgatag catgggctcc
     5701 ctcgagggcg ctcgggacac ttcatgaccc tggtccgacg cgcggtgctg cttctcgccg
     5761 gcctcgtcgc cgccggcgcg gccgcggtgc cgatgtctgc cgtagccgcc caggcgccca
     5821 cgagcgtgac cgtgaccacg ccgtccggcg acgtccggct gatcgccgat cggctggagg
     5881 agatcggcgc cgacgatctc gtcatcgcga cgggcaacgt cgaggtcacc cgcggcacgc
     5941 agcgcctcac cgcggaccgc gtcgagctga accgggcgac gggcgacgtc gtcgccgagg
     6001 gccgcgccgt cttctacgat ggcgacgacc gcctcttcgg cgagcgcatc gagtacaact
     6061 accgcaccgg caccggcgtc gtgcacgacg gcgccgcccg ggccgcgccc tactaccgca
     6121 tcgccggcga gcgcatggag cgactgggcg agggccgcta tcacgtgcgc cgcggggtct
     6181 tcacgacgtg cgaggacgac ccgccgacgt ggtcgttccg cttcgggagc gccgacgccg
     6241 acatggagtc gtacctgtac ggcaccgagg cgtccttctg ggtgaagcgg ttccccctga
     6301 tcccgttcgt gcccttcttc gccgccgcca tccggcgcga gcggcagacg gggttcctct
     6361 tcccgcgctt cggcagctcc tcgcgcaagg gcacctatct cgagctgccc ttcttctggg
     6421 ccatctccga cagccaggat gcgctcatca cgctcgacgc ctacggggac atcggcctcg
     6481 gcgccaacct cgaataccgc tacctcctgt cgcgcacgca ttacggctcc gtcaacggct
     6541 tctacgtcaa cgagttcgag cgaagcgacg acctgcagga cggtgtcgac gacaatcgcg
     6601 gctggtggtc gatcgagcac cggtggagga tcggcccccg cttcgacctg accgtggacg
     6661 tcaatggcgt ctccgacgac cggctgttcc gcgactacgg cgatcccctg gccacccggg
     6721 catcccagcg cgtggagtcc aatatcttct tgaccaggtc ctggcccacc gccaacctgg
     6781 ccggtaacct gttcttctac cgggacctca ccacgccgga agccgtggag ctgcaccgcc
     6841 tgcccgacat ccgctacgcg gcgccccggc agccgctgcc gtggctgccc gcggcgagcc
     6901 gcctgctcgg cgagctgaac gcgcagtacg tcaacttcgt gcgcgacgtg ggctccgagg
     6961 ggcagcggct cgacgtgcag ccgatcctct cccggccgtt ctcgctcggc ggctacgtca
     7021 ccctcacccc cttcgtcggc ggccggttca cggcctacga caagacggtc accggcacgc
     7081 gcgcggtcag cagcggcgcg gtcaccgtgg agaccaccga cgacgagccg cggctccggc
     7141 ggctcgtgca gctgggcggc gacgtcgagg cgcgggcctc gcgcgtgtac gaggtgggcg
     7201 gcttcggcgg catcgacgcc gtcctgcact cgatcgaacc gcgcgtgaac tacaccttca
     7261 tcgagggctc cgacctgctg cggcagacgg gaccggacac gttccggccc aatcgcctgc
     7321 cgcagtggga cgccgtcgac gcgatctccg agacgagcct gatcacctat tcgctgacca
     7381 accgcgtgcg ggggcggacc gtggcgccgg ccggcaccga gcccctccgc tgggaggcgg
     7441 tccgggtcgc gctcgggcag acgtacaacc tccacgacga cgaccggcca ttcggggacg
     7501 tcctcggcat cctcatcgtg aaccccggcc agatcctcgg cttccggagt gacgcgagct
     7561 acaacgtgta cggcaacggg ctgacgaccg ccaccgccga cgtcaccgtc gaggtcccgc
     7621 ggttccaggc ggccgtgggc gcgcgggtgg cgcgcgaccg tgaggtcgac ctctccggcc
     7681 gggtgcaaac cacgacccgg cagtccttcc tgcagaccac gatgcgggcc gagctcttcc
     7741 cgtgggccgt cgtgcggctc gagagcaact gggacgtcaa gcagaacgtg ttcgtcgaga
     7801 accggtacgg cgtggatctg cgctggcagt gctgggcgtt cacgctcgag tacgtcaggc
     7861 gtcacaacga cgaggacgag gtccgcttcg cgctgaacct gctcggcgtg ggccagccgc
     7921 tgcagttcgg cagcaggctc cagacctccg gcgggctcaa cacgagcggt gacgggcgca
     7981 tccggtgatc ccgcacatcg acctcggccg ccagcacgcc gggctccggg acgagctgct
     8041 cgccgcgatg gcccgcgtgc tcgactcctc gcgcttcgtg ctgggcggcg agggcgaggc
     8101 gctcgagcgc gagctggcgg ccctgaccgg cgtgcgccac gcgatcggcg tcgcctccgg
     8161 caccgacgcg ctccggctct cgctcgccgc gctgggggtg gggcccggcg acgaggtcat
     8221 cacgagcgcg ttctcgttcg tggcttcggc ctcgaccatc gtgatgaccg gggccacgcc
     8281 ggtcttcgtc gacatcgatc ccgagaccta cgcgctcgac gtggccgccg ccgagcgcgc
     8341 cgtgacgtcg cggacgcgtg cgatcgtgcc ggtgcacctc tacggtcacc cggcggccat
     8401 ggacgcggtg gcggcgctgg cgcgcgggca tggcctggtc gtgatcgagg acgctgcgca
     8461 ggcgatcggc gccgcctggc aggggcgcgc cgtcggcacg tggggcgaca ccgcgtgcct
     8521 gtcgttctac ccgacgaaaa acctcggcgc gtgcggggac gccggcatgg tcctcaccgg
     8581 acgcgacgac ctcgcggagc gcctgcgacg gctgcgccac cacggcgacg gcgggcgcta
     8641 ccgccacgtc gagctcgggt acgcgagccg gctcgacgag ctgcaggcgg cgatcctccg
     8701 cgtgaagctg cgccatctgc aggactggac cgaggccaag cgtcggctgg cggcgcgcta
     8761 cggtcggctg ctcgccggcc tgccgctggg acttccggtg gaacgtcccg gcgcgcgtca
     8821 cgtgtatcat ctctacaccg tgcgccatca gcggcgggac gcgctggcca aggccctcgc
     8881 cgatctcggc gtgggcaccg ccgtccacta ccccatcccg gtgcccgacc agccgatgtt
     8941 cgcgggcgcc cacgctgcag gacggaccgg agggcgagac gagcagcgcc gcggcgcgag
     9001 ccgcgccgcc gagacgtccg gatcccgcag gcggagcgcg tccgaggcga gtccgaccga
     9061 atccggcgag cgtcgctggc ccgaggcgtg gcgggccgcg cgcgaggtgc tcgcgctgcc
     9121 ctgcttcgcc gagctgacgg acgacgagca agaccgtgtg gccgccgccg tccgcagtgc
     9181 gctgcagggg acgtaggcac gtgacttccg tccccgagca ggcgccgaac gtgatccggc
     9241 tataggcgcc gtgctcacgg cggtctggct gtcgctccgg ccccgccagt gggtcaagaa
     9301 cctcttcgtc ttcgccggcc tcatcttcgg ccaccagctc ctgacaccgg cgatcgggcc
     9361 ggcgctggcg acgttcgcga tcttctgcgc gctgtcgggc gccgtctacc tggtcaacga
     9421 cgtcgccgac cgcgacaagg accgccagca cccgctgaaa cgccggcgcc cgatcgcctc
     9481 gggccggctg ccggccagcg tcgccgtggc cgtcgccgtg gtcctggccg tcgcgggcgt
     9541 gctggcgagc gcggccatcg agcggcgcct cgccgccgtc gccgtcgcct acgccgcgct
     9601 gctcgtcgcc tactcgctgt ggctcaagca cctcgtcatc gtcgacgtga tcgtggtcgc
     9661 cgtcggcttc gtgctgcgcg cggtcgccgg cgccgtggcc gtcgacgtgg agatctccgg
     9721 ctggctcctc atctgcacgg tcctgatcgc gctcttcctg gccctcggca agcgccgtca
     9781 cgagtacctc acgctcggcg cggaggcggc cggccaccgg cccatcctcg ccgagtacag
     9841 tgccgggctg ctcgaccaga tgatcgccgt ggtgacagcc tccacgctga cggcctacgc
     9901 gctctacacc atgtcgccgg agaccatcgc caagttcggc acccggctcc tgccggcgac
     9961 gctgcctttc gtgctctacg gcatcttccg gtatctctat ctgctctacc ggaagcagat
    10021 gggaggcagc ccgtcggacc tgttcctcag cgaccggccg ctcctggtca acacgctgct
    10081 gtggatgctg gcggtcgtgc tgatcatcta ctggtagctg ccgggaacgg cgtggccgcg
    10141 ccagggccct gaggcttgcc tcggagtgcc cgagagtcga gcgtcggcga acccggtgcg
    10201 atcgatgatg tccgcgtggg ggacaccctg cggcatggat cgtcggtggc tgcccggtgg
    10261 gtctcggagg ggccgtcgag gccgccgccc acgggtagaa tgagggtcca atgacgagga
    10321 gccaggtcgc cgtcctgcgt acgcgccccg cgacggtcgt cgaggactac gggcggctca
    10381 tgcgcctggt gaagtacgac caggtgctgt cacgcgacac ggacctgatc gtgaagctca
    10441 acctgtcgtg gacgaagtac ttcccggcgt gctcgagcca gccgtggcag gtcgacggca
    10501 tcctcgccac cctgctcgcc gacggctacg accgcgcacg gatccttccc gtcgagaacc
    10561 gcaccgtggt caccgatccc gtgcagggct gccgcaacaa caagtgggag ccggtgctgc
    10621 ggcgccacgg gctgcccttc gtcccgctca ccgacgtgga gtggacggtc tacccgttca
    10681 agacgccgct gctcaagctc aacgacatct tcccggaagg tatccagatc ccgaagatgt
    10741 acatcggcaa gaacgtctgc cacgcgccga cgaccaagac gcacggacac acgacgacca
    10801 ccggggccat caagaacgcg ttcggcgggc ttctcaagga agtccggcac tacgcgcacg
    10861 agttcatcca cgaggtcatg gtcgacctca tgctcatgca gcgcgagctg caccccggcg
    10921 tgatcgcggt gatggacggc acggtgatgg gcgacggcgc gggaccgcgc acgatggtgc
    10981 cgcgcgtggg caacctgatc ctcgcgtcgg cggaccaggt ggccatcgac gcgatcgccg
    11041 cgaaggtgat gggcttcgac ccgctcacga tcccctatct ccgcatgtgt cacgagcgcg
    11101 ggctcggtgt cgcggatccg cgcgagatcg agatcgtcgg cgatcccgac gccgcggagg
    11161 tgtccatggg cttcacgacc agccgtagcc tcgtcatctg gggcgaccag ctcatccgcc
    11221 gcggcccgct ccgtccgctc aagcggctgc tgctccactc gcccctcgtc gtctgggcgc
    11281 cgttggcgtc gaacgtgtac cacgactggc tctggtatcc gacggtcggg cgctcgcgca
    11341 tccgcgcctt cgccgggacg ccgtgggggc ggcacttcga gacgtactga ctacatgggg
    11401 gccccgacat ggcccccaag ccccccaggg tggctcggca cgccccggcg aagccgtggc
    11461 gcgcctcggt ctcgcgcgca gcggccccga ccgagcgctt ccttcgcgcg cgctgaccgc
    11521 gcgcagcgcg cggtagaatg acccgctgtg accgctgcat ccccggagac cgtccgcgtg
    11581 cgcttcgcgc ccagccccac cgggcacctg cacgtcggtg gcgcccgcac ggcgctgttc
    11641 aactggctct acgcccgcca ccaccgcggc gcgttcattc ttcgcatcga ggacaccgac
    11701 cgctcgcgct cgaccgacga gaacatcggg gccatcgtcg aggccctcga gtggctgggg
    11761 ctcgactggg acgaggggcc gccggcgccg gggtaccgcc agaccgagcg gctcgagctg
    11821 taccgcgcgc acgcggcgcg gctgctggcc gcgggccgcg cctactactg cgactgcccg
    11881 cccgaggcgc t
//
LOCUS       DATIAZ010000182        10299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_46653, whole genome shotgun sequence.
ACCESSION   DATIAZ010000182 DATIAZ010000000
VERSION     DATIAZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46653"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1237)
                     /locus_tag="VLF19_06430"
     CDS             complement(47..1237)
                     /locus_tag="VLF19_06430"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92924.1"
                     /translation="MSLDGLRVLDLSRIIAGPYAATLLADLGADVVKLERPGQGDDLR
                     HWRGGGGMSPVFAAINRNKRGVAVDLSHPEGARVAFELARHADVVVENFLPGAAERLG
                     LGYGAVSAANPAVVYASVTGFGQTGPYAHRAGYNTIAQGMSGLMGLTGMPGHPPTKVG
                     GSVADLAAAYLTFGGVCAALVTRFRTGKGQHLDVSLLSATLGLLPDPVAHFFAGGGVR
                     PRREGNRNPNLSPAEAFQTKDGLLNVVIMNADQYGRFCAGIGDADLGADPRFATNDGR
                     VANREEFRARVERALNGGTTAEWVERLGRVGVACGPIYELDEVFTDPQVHHLGLVTEV
                     DQPGLGPVRMLGFPYRTTAAPATVRRAAPRLGEHTAEVLEESGLSRAEIERLARSGAV
                     TLGG"
     gene            complement(1234..1962)
                     /locus_tag="VLF19_06435"
     CDS             complement(1234..1962)
                     /locus_tag="VLF19_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSE92925.1"
                     /translation="MPDNVVVARDGAIATVTLNRPDRRNALSDAMLIELAAALTELRD
                     DVATRVVIVTGAPPVFSAGADAGLRASMSEAEKREAFGRRKSQFRRLFERANTLLENL
                     EQPTVVAINGHAVGGGWGLTLACDFRVASAEAQLWIPEVDLGVPLGIASTTRFVRLVG
                     PARAKEIIMECRRYSADEAHRMGLVHRVVPADAVLKEARALAERLAVKPFRPLAETKA
                     RINAIARTGIPEVNAMTEGFLARE"
     gene            complement(2314..3018)
                     /locus_tag="VLF19_06440"
     CDS             complement(2314..3018)
                     /locus_tag="VLF19_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92926.1"
                     /translation="MTVLADLPLQIDPDEVLRFQGYKRGIDAPGPEVLALFHEALALG
                     RDLIAPRAAVRWAPVQRQAADRLEVDGLILAIPDVARHWGEVAEVAGAVVTIGDALER
                     RVGELWDARELPLAAMLDSVGSGAVESLAEYLNDALCQQGLERGVKVTNRISPGYAGW
                     DVAEQRLLVAMCPPGVIGVAVNDACFMTPAKSISLLVGAGPHARVDHYFSQCARCWMR
                     DCAYRRVPMRRAIRGE"
     gene            complement(3077..4006)
                     /locus_tag="VLF19_06445"
     CDS             complement(3077..4006)
                     /locus_tag="VLF19_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HSE92927.1"
                     /translation="MTARGREMLARVRAGEILVFDGGYGTMLFAAGLLNGACPELWND
                     THAGVVAGIHKGYFDAGSQIVETNTFGGTRLKLDEYRIGDRTRDLNVKGARLARSVAP
                     PEGYVAGSIGPTSRLPQDYALDQGVTDEEYETTFREQAEALAEGGVDLFAVETMMFPQ
                     EATAAIRACKAAAAELPVMATMFFQYEEGHDRDRTMWGESPTEVAKNLLAAGADVVGM
                     NCGRGPDRAIAIIREMRAVTDAPLVAYPNAGLPVTTGDTVTYELGPEAMAAEYPALLG
                     AGCNIVGACCGSNPEHIRLIGEVVRSRRVGGRR"
     gene            complement(4003..4671)
                     /locus_tag="VLF19_06450"
     CDS             complement(4003..4671)
                     /locus_tag="VLF19_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HSE92928.1"
                     /translation="MSDPFDYPTMARALIIGDKDTVARKTREGLELSMAPKDLIFKGL
                     IPGMDVVGEKFRRNEYYVPQVLLSARAMYAGLDLLKPLITAAARPDDNLGVVVIGTAQ
                     GDLHDIGKNLVAMMLEGAGFKVVNLGRDVAPEKFVAAVQEHGADIVGISALMTTTMPA
                     MKRTIDALTKAGMRGRVKVMVGGAPVSQAFADEIGADGYARDSTLAVAKAKTLVGAPE
                     KVAR"
     gene            complement(4807..6861)
                     /locus_tag="VLF19_06455"
     CDS             complement(4807..6861)
                     /locus_tag="VLF19_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HSE92929.1"
                     /translation="MVTLRTLLAGFAAAVLTVGLALLGALETSELGTFDRLFQLRGTR
                     SPVTPIVIVTIDEDSFDELNMQWPFPRAVHAQLVSALASGSPIAIGFDVLFPEPSSRG
                     PEDDRALGEAVKKAGNVVLGAAITHVSEGFYNKTDLNIPVPEVREGAAGVAPVNVQVD
                     QDGHLRRAVIRNRVEGEVLESWDIVLYRMARAAGIPAAPLPDDSEIIINYRGGPRTFP
                     WVPYHRVLSGDVPPEAFRDTIVLIGATTKVLQDIFSTPFARAREMPGVEIHAHVLDTL
                     VRGDRMRAVPLEVALAVAVAAAFAAAWLASWLRVFRAFVAVLALWVILAVVTVVAFAL
                     GNVWFRGIGVTLGLVLGYGITVVDNYVREQRERRRLSQFFSPDVLAEIVRHPTDAALG
                     SSRRRITVLFSDIRGFTSLSEKVEPEQVAEMLREYLSEMTEVVFRHGGTVDKYIGDCI
                     MALYNAPFDDADHAANAIKTGLELQERTIGVSARWQARLGEPIRNGVGINTGDAVVGA
                     MGSRQRLEYTAIGDTVNLASRLESLTKEYGTGIIISESTYEQVKGRFLTRQLGSVFVK
                     GKARPVKIYAVIAGDIRKYPRTALDAAATIVVTGGEERWVVRTRDASEGGVALVGLPS
                     EVAEGAMVEVRCEGGDLPKPITAQGRVAWRRDDLTGVEFTTVDPDAAPVLSDLVTSRK
                     QP"
     gene            6903..7667
                     /locus_tag="VLF19_06460"
     CDS             6903..7667
                     /locus_tag="VLF19_06460"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HSE92930.1"
                     /translation="MKIRVLGAYGAEGLGQRPSAFLVDERTLIDAGSVTGALTVPEQL
                     EIEHVLVSHSHLDHIAGLAFLTETLACCNARRPLTITSIEPVVDALRNAVFNNVVWPD
                     FAQIPSAHEPIIRYRKLVAEAEQRVGDLWVTPVPVSHTVPAAGFIVHDGTRGFVYSGD
                     TGPTDALWKAMRGLTGVRAVILECAFPNRLRGLADAARHLTPELIERELDKLPADVPV
                     WVFHIKPQFHQETAEELSRLDGGRVLTLEQDKIYDV"
     gene            complement(7706..8824)
                     /locus_tag="VLF19_06465"
     CDS             complement(7706..8824)
                     /locus_tag="VLF19_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE92931.1"
                     /translation="MSSRPLVDIRLEALRTRREPPPCLEDVNVHIRYGEFFTFLGPPH
                     SGKTAVLRAIAGFLPLVAGRVLVDGQDIGSLSPARRGVGFVFHQGALWPHMTAREHVA
                     FGLRQMAMDAAEVDRRVGVVLRRLGLAAVADRRPRALSLHEHRRLALARALAVEPRVL
                     LLDEPLAHLDPTPRKTLRLELAHLHRDLAVTTVCATRDAADALALSDRIAVMQAGRVV
                     QVGEPEQLYRRPTAREVARAFGPANFLAVRVVEVRELGVVVETEAGDRVPVAGIGAFR
                     EGSHGVLVLRPETLTLVDAAMARGPGIPGRVAMRVFEGARYVYEIDIGASAAVRVELP
                     AGDTALYRVGDRVRIEISSDTVVLLPADGRDAGDGPGA"
     gene            complement(8821..8967)
                     /locus_tag="VLF19_06470"
     CDS             complement(8821..8967)
                     /locus_tag="VLF19_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92932.1"
                     /translation="MRFLPVALLFGTFAWSFVYVSLPVLALIGLACVPAVTLREVAAG
                     RERA"
     gene            9184..9579
                     /locus_tag="VLF19_06475"
     CDS             9184..9579
                     /locus_tag="VLF19_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92933.1"
                     /translation="MSRSARPAIVHFPLHRLALCLDCEMCFEIGPDRCRACGSETWFT
                     LARFLELRGEPNMRQLIVVSRQRPKLYERLKRAFAGNRSVEVILDRREGDPPQGAENG
                     YVRPLDRRTADQVRTAGWTVVRRSAPASP"
     gene            complement(9627..9968)
                     /locus_tag="VLF19_06480"
     CDS             complement(9627..9968)
                     /locus_tag="VLF19_06480"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSE92934.1"
                     /translation="MRSSQDIQLERMSTINRRRYERTPVAWPVRVWADDGAVSGITED
                     VSPFGVCIVTARPASVRMGRSYRLDVLAGFDSPRSIVGEAKHADPKGRIGFETRWRWE
                     PRRSFAATAAP"
     gene            10060..>10299
                     /locus_tag="VLF19_06485"
     CDS             10060..>10299
                     /locus_tag="VLF19_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92935.1"
                     /translation="MPGLEEVLEAHGPVATQERDFEFLLRDMLQHRRLVGDPSNALDA
                     EPLPAPASDRPTVLVVDDDATLLATLADMLGRAFRV"
BASE COUNT         1495 a         3752 c         3602 g         1450 t
ORIGIN      
        1 ccggcacggc atgtcctgga tcaggatgcc ccgctgggca gcggcctcat ccgccgagcg
       61 tcaccgctcc cgaccgagcc agccgctcga tctccgcacg cgacaggcca gactcttcca
      121 ggacttccgc ggtgtgctct cccagacgcg gagcggcccg tcgcacggtc gccggcgccg
      181 ccgtggtgcg atacgggaag cccagcatgc gcaccgggcc gagcccgggc tgatcgacct
      241 cggtcacgag cccgaggtgg tgcacctgcg ggtcggtgaa gacctcgtcc agctcgtaga
      301 ttggcccgca ggcgaccccg accctgccga ggcgctccac ccactcggcg gtggtcccgc
      361 cgttcagcgc acgctcgacg cgggcgcgga actcctcgcg gttggccacg cgcccgtcgt
      421 tggtggcgaa gcgcgggtcg gcgccgaggt cggcgtctcc gatgcctgca cagaaccgcc
      481 cgtactggtc cgcgttcatg atgacgacgt tcagcagccc gtccttcgtc tggaacgcct
      541 cggccggcga gaggttgggg ttgcggttgc cctcgcggcg cggccgcacg ccgccgccgg
      601 cgaagaagtg ggcgacgggg tcgggcagca ggccgagggt cgctgacagc aggctcacgt
      661 cgaggtgctg tcccttgccg gtccggaaac gcgtcacgag cgccgcgcag acgcccccga
      721 acgtcaggta cgccgcggcc aggtcggcga cggagccgcc caccttggtg gggggatggc
      781 ccggcatgcc cgtcagcccc atgagcccgc tcatgccctg cgcgatcgtg ttgtagccgg
      841 cgcgatgcgc atagggtcct gtctgaccga acccggtcac ggacgcgtaa accaccgccg
      901 ggttcgccgc gctcacggcg ccgtagccga ggccgaggcg ctccgccgcg ccgggcagga
      961 agttctccac gacgacatcg gcgtgccgcg ccagttcgaa ggccacgcgc gccccctcgg
     1021 ggtgcgagag atcgacggcc acaccccgct tgttgcggtt gattgccgcg aagaccgggc
     1081 tcatgccgcc gccgccgcgc cagtggcgca ggtcgtcccc ctgccccgga cgttcgagct
     1141 tgacgacgtc ggcgccgagg tcggcgagca acgtcgccgc gtaggggccg gcgatgatgc
     1201 gggagagatc gaggacgcgc agcccgtcga ggctcactcg cgcgcgagaa agccttcggt
     1261 catcgcgttg acctccggga tgccggtgcg cgcgatggcg ttgatacgcg ccttcgtctc
     1321 cgccagcgga cggaagggct tgacggccag gcgctcggcg agcgcgcgcg cctccttcag
     1381 cacggcgtcg gccggcacga cccggtgcac cagccccatc cggtgcgcct cgtccgccga
     1441 gtagcggcgg cactccatga tgatctcctt ggcgcgcgcc ggccccacca gccgcacgaa
     1501 ccgcgtcgtc gacgcgatgc cgagcggcac ccccaggtcc acctcgggga tccataactg
     1561 cgcctcggcg gacgcgacgc ggaagtcgca ggcgagcgtg agcccccagc cgccaccgac
     1621 ggcgtgcccg ttgatggcga ccacggtcgg ctgctcgagg ttctcgagca gcgtgttcgc
     1681 ccgctcgaac agccgccgga actggctctt ccggcggccg aaggcctcgc gcttctcggc
     1741 ctcggacatc gaggccctga gccccgcgtc ggcgcccgcc gagaacaccg gtggtgcgcc
     1801 cgtgacgatc accacgcgcg tggcgacgtc gtcgcgcagc tcggtcaacg ccgccgcgag
     1861 ctcgatcagc atcgcgtcgc tgagcgcgtt gcgccggtcg ggccggttca gcgtcaccgt
     1921 ggcgatggcg ccatctctgg cgacaacgac gttgtccggc atcgtgtcgc ggacgctacc
     1981 acacgaccgc gccgcgccgc aacgccgcgc cccttggcgg cgcctcgccc ggtcgtgcgc
     2041 gccggcgctc gcacacgaca ccgcgccgga gccatcgccc tctcagaccg gatccgccga
     2101 cggacgactc agagcgtgtg aaagaaaccg cgtggtcgga ggcttggggg cgtccgagcc
     2161 cccacgaacg tgtgtcgagg ggcggcgcca cagcgccccg accgccgagg gcttggaggc
     2221 cccggagccc cacgaaggtg ttgtcgaggg gcgccacggc tgtgccgggg accagagcgt
     2281 cggggcttcg gcgcgcccgg agcccccacg tattcattct ccgcggatcg cccggcgcat
     2341 cggcactcgc cggtaggcgc aatcgcgcat ccagcaacgg gcgcactggc tgaaatagtg
     2401 gtcgacccgg gcgtggggcc cggcgccgac gaggagcgag atggacttgg ccggcgtcat
     2461 gaagcaggcg tcgttgacgg cgacgccgat gacgcccggc gggcacatgg ccaccagtag
     2521 ccgctgctcg gcaacgtccc agccggcgta gcccggactg atgcggttgg tcaccttcac
     2581 gccgcgctcg agcccctgct ggcagagcgc gtcgttgaga tactcggcga ggctctcgac
     2641 cgcgccggag cccacgctgt ccagcatcgc cgccaggggc agctcgcgtg cgtcccacag
     2701 ctcccccacc cgccgctcga gcgcgtcgcc gatcgtgacc acggcgccgg cgacctcggc
     2761 cacctcgccc cagtggcggg cgacgtcagg gatggccagg atcagaccgt cgacctcgag
     2821 gcgatccgcc gcctggcgct gtacgggcgc ccagcggacg gccgcgcgcg gtgcgatcag
     2881 gtcgcggccc agcgcgaggg cctcgtggaa gagcgccagc acctcgggcc cgggcgcgtc
     2941 gatcccgcgc ttgtagccct gaaagcgcag cacctcgtcg ggatcgatct ggagcggcag
     3001 gtcggcgagg acggtcacgt cccgcgtttc cgtgcgacct cgaaccgttc tcaccgctgg
     3061 cctcgggcgt ggacgcctac cggcgtccgc ccaccctgcg gctgcgaacc acttcgccga
     3121 tcaggcggat gtgctcgggg ttggagccgc agcaggcgcc gacgatgttg cagccggcgc
     3181 cgagcagcgc cgggtactcg gcggccatcg cctccggccc cagctcgtag gtgacggtgt
     3241 cgcccgtcgt gaccggaagc cccgcgttcg ggtacgccac cagcggtgcg tcggtgacgg
     3301 cgcgcatctc gcggatgatc gcgatcgccc ggtcgggccc acggccgcag ttcatgccga
     3361 ccacgtcggc gccggcggcg agcaggttct tggcgacctc cgtcgggctc tcgccccaca
     3421 tcgtccggtc gcgatcgtgg ccctcctcgt actggaagaa catcgtggcc atgacgggca
     3481 gctccgccgc cgccgccttg caggcgcgga tggcggcggt cgcctcctga gggaacatca
     3541 tggtctccac ggcgaagaga tcgacaccgc cctcggcgag ggcctcggcc tgctcgcgga
     3601 acgtcgtctc gtactcctcg tcggtgacgc cctggtcgag cgcgtagtcc tggggcaggc
     3661 ggctcgtcgg cccgatggag cccgccacgt agccctcggg cggggcgacc gagcgcgcca
     3721 gccgcgcgcc cttgacgttc aggtcgcgcg tgcggtcgcc gatccggtac tcgtcgagct
     3781 tgagtcgcgt gccgccgaag gtgttggtct cgacgatctg gctgcccgcg tcgaagtatc
     3841 ccttgtggat accggccacc accccggcgt gggtatcgtt ccagagctcg gggcaggcgc
     3901 cgttcagcaa gccggccgcg aacagcatcg tgccgtaacc gccgtcgaag accaggatct
     3961 cgccggcgcg cacgcgcgcc agcatctcgc ggccgcgcgc cgtcacctgg ccaccttctc
     4021 cggggcgccg accagggtct tggccttcgc gacggcgagc gtggagtcgc gggcgtagcc
     4081 gtcggcgccg atctcgtcgg cgaatgcctg gctcaccggc gcgccgccga ccatgacctt
     4141 cacccgccca cgcatgccgg ccttcgtcag cgcgtcgatc gtccgcttca tggccggcat
     4201 cgtggtcgtc atgagcgccg agatcccgac gatgtcggcg ccatgctcct gcaccgcggc
     4261 cacgaacttc tccggggcga cgtcgcgccc gaggttgacg actttgaagc cggcaccctc
     4321 cagcatcatg gccacgaggt tcttgccgat gtcgtgcagg tcgccctgcg cggtgccgat
     4381 gacgaccacg ccgaggttgt cgtcgggccg cgcggcggcg gtgatcaggg gcttgaggag
     4441 gtcgagcccg gcgtacatgg cgcgggccga gagcagcacc tgcggcacat agtactcgtt
     4501 gcggcggaac ttctcgccga cgacgtccat gcccgggatg agccccttga agatgaggtc
     4561 cttcggcgcc atcgagagct ccaggccctc ccgggtcttg cgcgcgacgg tgtccttgtc
     4621 gccgatgatc agcgcgcggg ccatcgtggg atagtcgaac ggatcgctca tggcattttt
     4681 ctagcacacg cccgcggtcg cagcaacgcg ccagaggcgc gtcggagccc cgaggcacgc
     4741 cacggctccg ccgcggtgtg ccgagcagcc tgggggcctg gaggccattt cggcgcctcc
     4801 atcgtcttaa ggctgcttgc gcgacgtcac cagatcgctc aggaccggcg ccgcgtcggg
     4861 atcgacggtg gtgaattcca caccggtgag gtcgtcgcgg cgccaggcga ctcggccctg
     4921 ggcggtgatc ggcttgggga ggtcaccgcc ctcgcaccgg acttccacca tcgcgccctc
     4981 ggccacctcc gacggcaggc cgacgagcgc caccccgcct tcactggcgt cgcgggtgcg
     5041 tacgacccag cgctcctcgc cgccggtcac cacgatcgtc gccgccgcgt cgagggccgt
     5101 gcgggggtac ttccggatgt cgccggcgat cacggcgtag atcttgacgg gccgggcctt
     5161 gcccttcacg aacaccgagc ccagctggcg ggtgagaaag cggcccttga cctgctcgta
     5221 cgtggactcg ctgatgatga tgccggtccc gtactccttg gtgagactct ccagccgcga
     5281 ggcgaggttc accgtgtcgc cgatcgccgt gtactccagg cgctgccgcg agcccatggc
     5341 tccgaccacg gcgtccccgg tgttgatccc cacgccgttg cggatcggct cgccgagcct
     5401 ggcctgccac cgcgccgaga ccccgatcgt gcgctcctgc agctccaggc ccgtcttgat
     5461 cgcgttggca gcgtggtcgg cgtcgtcgaa cggcgcgttg tacagcgcca tgatgcagtc
     5521 gccgatgtac ttgtcgaccg tgccgccgtg ccggaagacc acctcggtca tctcgctgag
     5581 gtactcccgg agcatctcgg ccacctgctc gggctcgacc ttctcggaga gcgacgtgaa
     5641 gccccggatg tccgagaaga gcacggtgat gcgccggcgg ctcgatccca gggcggcgtc
     5701 ggtcgggtga cgcacgatct cggcgaggac gtcgggcgag aagaactgcg acagccggcg
     5761 ccgctcccgc tgctcccgca cgtagttgtc cacgaccgtg atgccgtagc cgagcacgag
     5821 gccgagcgtc acgccgatgc cccggaacca cacgttcccg agggcgaagg ccaccaccgt
     5881 gaccaccgcg aggatgaccc acagcgccag caccgccacg aacgcgcgga acacgcgcag
     5941 ccaggaggcg agccaggccg ccgcgaacgc cgcggcgacc gcgacggcga gggcgacctc
     6001 cagcggtacc gcacgcatgc ggtcgccgcg gaccagcgtg tcgaggacgt gggcgtggat
     6061 ctcgacgccg ggcatctcgc gcgcccgcgc gaagggcgtg gagaagatgt cctgcagcac
     6121 cttcgtggtg gcgccgatca gcacgatcgt gtcgcgaaag gcctccggtg gcacgtcgcc
     6181 gctgaggacc cggtggtacg gcacccaggg gaacgtcctg gggccgccgc ggtaattgat
     6241 gatgatctcg ctgtcgtcgg gcaacggcgc cgccgggatg ccggcggcgc gggccatgcg
     6301 atagagcacg atgtcccacg actcgaggac ctcgccctcc acgcggttgc ggatcaccgc
     6361 ccggcgtaga tgaccgtcct ggtccacctg cacgttgacg ggcgccaccc cggcggctcc
     6421 ctcgcgcacc tcgggcaccg gaatgttgag gtcggtcttg ttgtagaagc cctcggacac
     6481 gtgggtgatc gcggcaccga gcacgacgtt gccggccttc ttgaccgcct cgcccagcgc
     6541 ccggtcgtcc tcggggcccc gtgacgacgg ctcgggaaag aggacgtcga agccgatcgc
     6601 gatggggctc ccggacgcga gcgcgctcac gagctgggcg tggacggcgc gcgggaacgg
     6661 ccactgcatg ttcagctcgt cgaacgagtc ctcgtcgatc gtcacgatga cgatgggggt
     6721 cacggggctg cgcgtaccgc gcagctggaa gagccggtcg aacgtaccga gctcggaggt
     6781 ctcgagagcg ccgagcaggg ccaggcccac cgtgagcacg gcggcggcga aaccggccag
     6841 aagcgtcctg agcgtcacca tgaagaacgg cccgaagccc gttccatgct accctatccg
     6901 ggatgaaaat ccgcgtactt ggggcgtacg gggccgaagg tctcggtcag cgcccctcgg
     6961 ccttcctcgt cgacgagcgg accctcatcg atgccggcag cgtcaccggc gccctcaccg
     7021 tgcccgagca gctcgagatc gagcacgtcc tcgtgtccca ttcgcacctc gatcacatcg
     7081 cgggcctggc cttcctgacc gagacgctcg cctgctgcaa cgcccggcgg ccgctcacga
     7141 tcacgagcat cgagccggtc gtcgacgccc tccggaacgc cgtgttcaac aacgtcgtct
     7201 ggccggactt cgcgcagatc ccctcggcgc acgagccgat catccgctac cgcaagctcg
     7261 tcgccgaggc ggagcagcgc gtcggtgacc tgtgggtgac gcccgtgccc gtcagccaca
     7321 cggtgcccgc ggccggcttc atcgtgcacg acggcacgcg ggggttcgtg tactccggcg
     7381 ataccggccc gaccgacgcg ctctggaaag ccatgcgtgg gctcaccggg gtgcgagcgg
     7441 tcattctcga gtgcgctttc ccgaaccgct tgaggggtct cgccgatgcc gcccgacacc
     7501 tcacgcccga gctcatcgag cgcgagctgg acaagctgcc cgccgacgtt cctgtctggg
     7561 tctttcacat caagccccag ttccaccagg aaactgccga agagctgtca cggctcgacg
     7621 gtggccgcgt cctcaccctc gagcaggaca agatctacga cgtttgacgt tcccggccgc
     7681 cctgcgcccg ccgaccgcgt gagcgtcagg cgcccgggcc gtcgccggca tcacgcccgt
     7741 ccgcggggag caggacgacg gtgtcgctgc tgatctcgat tcgaactcga tcgcccaccc
     7801 ggtagagcgc ggtgtcgccc gccggcagct ccacgcgcac cgcggccgag gccccgatgt
     7861 cgatctcgta gacgtagcgt gccccctcga acacgcgcat ggcgacccgg cccgggatgc
     7921 ccggcccgcg ggccatggcg gcgtccacca gcgtcagggt ctcgggacgg agcacgagca
     7981 ccccgtggct accctcgcgg aaggcgccga tgccggcgac gggcacccgg tcgccggcct
     8041 ccgtttccac cacgacgccg agctcgcgca cctccacgac ccggacggcg aggaagttcg
     8101 cgggcccgaa ggccctggct acctcgcgcg cggtgggccg ccggtagagc tgctcgggct
     8161 caccgacctg cacgacccgg ccggcctgca tcaccgcgat gcgatcggag agggcgagcg
     8221 cgtcggcggc gtcccgcgtc gcacagacgg tggtgaccgc caggtcgcgg tgcaggtgcg
     8281 ccagctcgag ccggagcgtc ttgcgcggcg tcggatcgag gtgggccagc ggttcgtcga
     8341 gcagcagcac gcggggctcg acggccagcg cgcgcgccag ggccagccgc cggtgctcgt
     8401 ggagcgagag cgcgcggggc cggcgatcgg cgacggccgc cagccccagg cgccggagga
     8461 ccacgccgac gcggcgatcg acttcggccg cgtccatcgc catctgccgc agtccgaagg
     8521 cgacgtgctc gcgcgccgtc atgtgcggcc acagcgcgcc ctggtggaac acgaagccga
     8581 cgcctcggcg ggcgggcgac aacgatccga tgtcctggcc gtccacgaga acccgcccgg
     8641 cgacgagcgg caggaacccg gcgatggcgc gcaggacggc cgtcttgccc gagtggggcg
     8701 gtcccagaaa cgtgaagaac tccccatagc ggatgtgcac attgacgtcc tcgaggcagg
     8761 gcggcggctc cctgcgcgta cggagcgcct cgaggcggat gtcgaccagc ggccggctgc
     8821 tcacgcccgc tcccgtccgg ccgcgacctc gcggagggtg acggcgggca cgcaggcgag
     8881 tccgatcagg gccagcacgg gcaggctcac gtagacgaac gaccacgcga aggttccgaa
     8941 gagcagggcg acgggcagga agcgcaccgg ggcgaatgtt agcagaccct gcggggacgg
     9001 acgcccagag tccgggcgcc cgcttcaggc caacgcccgc caggccgacg tcgtcggatg
     9061 cgacggcggc aaggcctcgc ctcgtcaaga ccgcgagatc tcgtgccgat accagaagcg
     9121 ggccgaggct cggccccagg aacagagggg acgatgatca ggcaactgcg acgtacaggc
     9181 gcggtgtcca ggtcggcacg accggccatc gtccacttcc cgctccaccg tcttgcgctc
     9241 tgcctcgact gcgagatgtg cttcgagatc gggccggacc gttgccgggc ctgcgggagc
     9301 gagacgtggt tcacgctcgc acgattcctc gagctccgcg gcgagccgaa catgcgccag
     9361 ctcatcgtcg tgtcgcgcca gcggccgaag ctctacgagc gtctcaagcg cgcgttcgcg
     9421 ggtaacagga gcgtggaggt catcctcgat cgccgcgagg gggatccgcc ccaaggtgcc
     9481 gagaacggct acgtgcggcc tctcgatcgc cggaccgccg accaggtgcg cacggcggga
     9541 tggacggtgg tgcggcggtc ggcaccggcc tcgccatagc gcgggcggcg gccggacgac
     9601 tcacggcccg gcgggtgccg cggggcttac ggcgcggccg ttgccgcgaa gctgcggcgg
     9661 ggttcccagc gccagcgcgt ctcgaaaccg atccgaccct tcgggtcggc gtgcttcgcc
     9721 tcgccgacga tgctccgggg cgagtcgaag ccggccagca cgtccaggcg gtaggagcgc
     9781 cccattctca cggacgccgg gcgcgccgtc acgatgcaca cgccgaacgg gctgacgtcc
     9841 tcggtgatac cggacaccgc gccatcgtcc gcccagaccc tcacgggcca cgccaccggg
     9901 gttcgctcgt atcggcgacg attgatggtg ctcatgcgct cgagttgaat gtcttgcgag
     9961 gagcgcatcg ctgtcaaggt tcaagtgcgc ccctgtgcgc ccggaagtcg gtccctgtcc
    10021 cctctccgcg tcgacaccgg ggctgccgat gactccggca tgcccggact ggaggaagtg
    10081 ctcgaggccc acggtcccgt cgccacccag gagcgcgact tcgagttcct gctccgcgac
    10141 atgctccagc accgccggct ggtcggtgat ccctcgaatg cactagatgc cgagccgctg
    10201 cccgcgccgg ccagcgaccg cccgaccgtg ctcgtggtgg acgacgacgc cacgctcctc
    10261 gcgacgctcg ccgacatgct cgggcgggcg ttccgggtc
//
LOCUS       DATIAZ010000183         7361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_836013, whole genome shotgun sequence.
ACCESSION   DATIAZ010000183 DATIAZ010000000
VERSION     DATIAZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836013"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VLF19_06490"
     CDS             complement(<1..292)
                     /locus_tag="VLF19_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92936.1"
                     /translation="MHRVLVIDQDRDAVQALGLACLSRDVGVALADNVCEGVRVLLSE
                     SISLVVVDAAQMRLAAHEHAELFDRVAPGVPVVVVVSPSTSLESRLALELAGF"
     gene            complement(581..1936)
                     /locus_tag="VLF19_06495"
     CDS             complement(581..1936)
                     /locus_tag="VLF19_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE92937.1"
                     /translation="MSRAIRILVADDERNLRELLVRELSRRGHEVQGVADGEAALERI
                     GEGGWDVVVLDMKMPRREGIEVLRELATQPESPQAIVMTGFQEVSTAVEAMKLGAYDY
                     LTKPAKIEELDVLVRKAAEKGQLLRDNVALRAHAPGAQPFAGIITKNPQMQDVLRIIE
                     RVAPTDSSVLILGESGTGKELVARAIHERSTRAERPFVPIHCGALPREVLESELFGHE
                     KGSFTGAINAKPGLIELADGGTLFLDEIGEMEPDSQVKLLRVLESGAFFRVGGTRPRR
                     VDVRVVSATNRDLAEAMKAGEFRQDLYYRINTIALQLPPLRDRRDDVALLARHFLESG
                     TSYGDRRLGAAALACLEAYPWPGNVRELQHAIERAVILAKGEEIQPADLPPEVVGGAP
                     VSAAAPAAGSLEAVERQHIIAVLRQVGGHRGKAAALLGIDPKTLYRKVLAYQIKTEEF
                     R"
     gene            complement(1933..4962)
                     /locus_tag="VLF19_06500"
     CDS             complement(1933..4962)
                     /locus_tag="VLF19_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSE92938.1"
                     /translation="MKIGRVFPDLLMQLSAVSPIEVGLAGMLRRLVGVTGAAAGALVF
                     RPPRGEALVVTAARGRVPPPLDAWLRGEAARPISGGRVSRGAVPALPGRHVVLRAPLG
                     APGAPMGALTLLGRRLGRPTLPVRFSRDLGTAIDRVWQAHRRTLRMRVLSEITRLGAS
                     SEPLEAVLDAFGEGLGALVRFDGVGVSLIDAERGDFTIVDVPAWSLHFGARRDVRMPL
                     AGTLLARVVDSGAPLRADDLAGADVPAASRAAFGARGYRSAVLAPLTVRGAVAGAVTV
                     AARLPGAFDDADLEIVAELAQPLAAAVEQRRLLEESSHRAEELAALLKTSRLVSARLE
                     LPAVLEAISRAATALTGGTGCGIGLIDAEGRRLAHAATHGFRSDEWRALSIAVGDGII
                     GRCAETGEPARVHDIARDPHSVRRDVDEREDIRSLLCVPMRAAGALIGVISAISTTPG
                     AFSAHDQAVLEAFAEQAGIAIHNARLYEERGRHARETRALLEAGRAVTASLDVQETIR
                     VIITEARSVLGAASCGLLTHDPVSGDLVGAASLDLPPEMVAQIRIRPDEGIGGRAFSE
                     RRPVQSEDLARDPRLRLPELTKGSALRSMLAAPLRVGDRAVGVIYVFRSDVHRFSPDE
                     EALLLALADQAAIALEHARLYRGLEDVVAERTRELDDQKRFVEVVLETLPIGVFVLDG
                     ELRVVRANGHGIRVLGCATPGGSRIVDLVPPDRQAGLSAFLRETFAAAAVRVHEQEVT
                     MAGEPRIFRLTAAPFESGVAHLVLLVEDVTLAKRLERQMLLTERLTTAGRMAAGVAHE
                     LNNPLATIAGCAEALLARAREAPHAGAPEMDDFRHYLGLIEEEAYRCKDITGSLLHFV
                     REPDSRRTPTHLDALVVKTVELLSHQKRFAGRRIVTEASPDLPRVTVNEGQLRQVFLG
                     IAANALEAMDAEGVLTIRTRRLRGEVEVEFEDEGPGIPDEVLSKVFDPFFTTKPPGQG
                     TGLGLAIAQGIVTDHGGRIDVTSRVGKGSIFRVALPA"
     gene            complement(4974..6581)
                     /locus_tag="VLF19_06505"
     CDS             complement(4974..6581)
                     /locus_tag="VLF19_06505"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HSE92939.1"
                     /translation="MSRPAMLDLKLEGATVIDGTGATGSRADVGVRDDVIAVVGDLSR
                     EAAGRTVNVAGRVLVPGFIDMHSHSDWRLWANRRAESKVRQGVTTEVVGNCGFSPAPV
                     STEHLEDLRSFALHVPKRLDFAWRSVGEYLDAFDAEGTALNVVQLVGHGTLRIAAMGF
                     ARRPATGDELTRMQRWLGQAIEDGAWGMSTGLIYAPGSFAATEEIVAVARAARGRGIY
                     ASHIRGEGATLLDAVREAIRVGGEADLPVQVSHVKAAGRPNWGKVPEALALIDAGCAE
                     GLDVMADVYPYTASSTTLRTLLPDWVLEGGIEAMLARLGDPTARARIRRDVESPDNGQ
                     SLVARVGWENVMVAAAPHRPDAEGRRLSEIARERGVDSLDAALELIADERGRGYVILF
                     QLDERDLRAALAHPRVMIGSDGSSLAPYGELGTGKPHPRSYGTFARVLGEYAREQRVL
                     TLPEAVHKMTGLPARRLGLKDRGVIRPGARADLVVFDSRRVADQATYQEPHRYAAGID
                     HVLVNGRFVVRDGEHTGSLPGKVLRLP"
     gene            complement(6578..>7361)
                     /locus_tag="VLF19_06510"
     CDS             complement(6578..>7361)
                     /locus_tag="VLF19_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSE92940.1"
                     /translation="VLRPAAYCGVVGVKPTHGAIPVDGVIPLAWSLDHVGAFARSVAD
                     AALILGVLMARELHPVAPRAPRLAVAGELLARCEPATAAQVQAVADRLARAGASVVEM
                     KLPASFADLAEAGRLVLEAEAATYHEESWRAHGGEYGREMGALVQAGLALPATAYVRA
                     NRARLRFRDEVLPLLAAADALLAPTAPAPAPAGLGWTGDASLCAPWTWAGVPAITLPS
                     GLADNGLPHAVQLVAAAGEEQRLLDAAQFCEEVLGFTSAPSL"
BASE COUNT         1016 a         2746 c         2666 g          933 t
ORIGIN      
        1 ggaatcccgc gagctcgagc gcgaggcggg attcgagcga cgtgctcggc gagaccacga
       61 cgacgaccgg aacgccgggc gccacgcggt cgaacagctc ggcgtgctcg tgcgccgcca
      121 gccgcatctg cgcggcgtcc accacgacga gagaaataga ttcgctcagc aagacccgca
      181 cgccttcgca gacgttgtcc gccagcgcca cacccacgtc ccggctgagg caggccagac
      241 ccagcgcctg cacggcgtca cgatcctggt cgatcacgag aacgcgatgc atcactcctc
      301 cctctctcgc cgacgatggg acatcggacg gacgacgtag gagcaaggcg aatgccacac
      361 cgcgtcagtc cgcaacctcc cggaatcatg gacggcggcc agtacgagca ccccgtgacc
      421 ggggcagaac gcctgaacac cggtgcctcg catcggccgt cgaggcgcgc gcgggcgcag
      481 tcgtgggcgt gccgaaggtc cggggggtac gtcgggcccc gtctggcgct ggtcgccagc
      541 acgcaatctc gaggcgcgtc gcggccacgc cggaggggtc tcagcggaac tcttcggtct
      601 tgatctggta ggcgaggacc ttccggtaca gcgtcttcgg atcgatcccg agcagcgccg
      661 cggccttgcc gcggtggcca cccacctggc gcaggaccgc gatgatgtgc tggcgctcca
      721 cagcctccag gctgcccgcg gccggcgccg cggcggacac cggggcgccg cccacgactt
      781 ccggcggcag gtcggcaggc tggatctcct cgcccttggc gaggatgacg gcgcgctcga
      841 tcgcgtgctg cagctcgcgc acgttgccgg gccacgggta ggcctcgagg caggcgagcg
      901 ccgcggcgcc gaggcggcgg tcgccgtagg acgtgccgga ctccagaaag tgacgcgcga
      961 gcagcgcgac gtcgtcccga cggtcgcgca gtggcgggag ctggagggcg atggtgttga
     1021 ttcggtagta gagatcctgc cggaactcgc cggccttcat ggcctcggcg aggtcccggt
     1081 tggtcgcgga gacgacgcgc acgtccaccc gccggggccg agtgccgccc acgcggaaga
     1141 aggcgccgct ctccagcacc cgtagcagct tcacctggct gtccggctcc atctcgccga
     1201 tctcgtcgag gaacagggtg ccgccatcgg cgagctcgat gagcccgggc ttggcgttga
     1261 tcgcgcccgt gaacgacccc ttctcgtggc cgaacagctc ggattccagc acctcgcgcg
     1321 gcagcgcgcc gcagtggatc ggcacgaacg gccgctcggc gcgcgtcgag cgctcgtgga
     1381 tggcgcgggc caccagctcc ttgccggtgc cgctctcgcc gaggatgagg accgacgaat
     1441 cggtcggggc cacccgctcg atgatcctca gcacgtcctg catctgcggg ttcttcgtga
     1501 tgatgccggc gaagggctgc gcgcccggcg cgtgggcgcg cagggcgacg ttgtcgcgca
     1561 ggagctggcc cttttcggcg gctttccgca cgagcacgtc gagctcctcg atcttcgcgg
     1621 gcttggtgag gtagtcgtag gcgccgagct tcatggcctc gaccgccgtg gagacctcct
     1681 gaaagcccgt catcacgatc gcctggggag actcgggctg cgttgccagc tcgcgcagaa
     1741 cctcgatgcc ctcacgccgg ggcatcttca tgtccagcac gaccacgtcc cagccgccct
     1801 ccccgatgcg ctccagcgcc gcctcgccgt cagccacgcc ctggacctcg tgcccccgcc
     1861 ggctgagctc ccgcaccagc agctcgcgca ggttccgctc gtcgtcggcg accaggatcc
     1921 ggatcgcgcg gctcatgccg gcagcgccac gcggaagatg gagcccttgc ccacccgcga
     1981 cgtcacgtcg atgcgccctc cgtgatccgt cacgatgccc tgcgcgatgg ccagcccgag
     2041 tcccgtcccc tgaccgggcg gcttggtggt gaagaagggg tcgaagacct tcgacaggac
     2101 ctcgtcgggg atgccgggcc cctcgtcctc gaactcgacc tccacctcgc cgcgcagccg
     2161 ccgcgtacgg atcgtgagca ccccctcggc gtccatggcc tccagcgcgt tggcggcgat
     2221 gccgaggaag acctggcgaa gctggccctc gttgaccgtc acgcgcggca ggtccggcga
     2281 ggcctcggtg acgatccggc ggcccgcgaa ccgcttctgg tgggagagca gctccaccgt
     2341 cttcaccacg agcgcgtcga gatgggtcgg cgtccggcgg ctgtcgggct cccgcacgaa
     2401 gtgcaggagg ctgccggtga tgtccttgca gcgataggcc tcctcctcga tgagaccgag
     2461 gtagtgacgg aagtcgtcca tctccggggc gccggcgtgc ggcgcctcgc gcgcccgggc
     2521 caggagcgcc tccgcgcagc ccgcaatcgt ggccagcggg ttgttcagct cgtgcgcgac
     2581 gccggcggcc atgcgcccgg ccgtggtcaa gcgctccgtg agcagcatct gccgctccag
     2641 acgcttggcc agcgtgacgt cctcgaccag caggaccagg tgcgcgaccc cggactcgaa
     2701 cggtgccgcc gtgagccgga agatcctggg ttctcccgcc atcgtgacct cctgctcgtg
     2761 cacgcgaacg gccgccgccg cgaacgtctc gcggaggaag gcgctcagcc ccgcctgacg
     2821 atccggcggc accaggtcga cgatccggga gccccccggg gtggcgcagc cgagcacgcg
     2881 gatcccgtgc ccgttggcgc ggaccacgcg gagctccccg tcgaggacga acacgccgat
     2941 cggcagcgtc tcgagcacga cctcgacgaa gcgcttctgg tcgtccagct cgcgcgtgcg
     3001 ctcggcgacg acgtcctcga gcccccggta caggcgcgcg tgctccagcg cgatggccgc
     3061 ctggtcggcg agcgcgagga gcagcgcctc ctcgtcgggc gagaaccggt ggacgtcact
     3121 acggaagacg tagatcacgc ccacggcgcg gtcacccacc cgcaggggcg ccgccagcat
     3181 cgagcggagc gcgctcccct tcgtcagctc gggcaggcgc agccgcggat cccgggcgag
     3241 atcctcgctc tgcacgggcc gacgctcgct gaaggcgcgc ccgccgatgc cctcgtcggg
     3301 cctgattctg atctgcgcga ccatctcggg gggcaggtcg aggctcgccg cgccgaccag
     3361 gtcgccggag acgggatcgt gcgtgaggag gccacaggag gcggcgccga gcacgctgcg
     3421 cgcctcggtg atgatcacgc ggatggtctc ctgcacgtcc aggctcgccg tcaccgcgcg
     3481 gcccgcctcg aggagcgcgc gagtctcccg cgcgtgtcgc ccgcgctcct cgtacagccg
     3541 cgcgttgtgg atggcgatcc ccgcctgctc ggcgaaggcc tcgagcacgg cctggtcgtg
     3601 cgcgctgaag gcaccgggag tcgtggagat ggcggagatc acgccgatca gggcgccggc
     3661 ggcgcgcatc ggcacgcaga gcagcgagcg gatgtcctcg cgctcatcga cgtcgcgacg
     3721 caccgagtgc gggtcgcgcg cgatgtcgtg gacgcgggcg ggctcgccgg tctccgcgca
     3781 gcggccgatg atgccgtcgc cgacggcgat cgagagcgca cgccactcgt cgctcctgaa
     3841 gccgtgcgtg gcggcgtgcg ccaggcggcg accctcggcg tcgatcaacc cgatgccaca
     3901 gcccgtgccg ccggtgaggg cggtcgccgc gcgactgatc gcctcgagca cggcgggcag
     3961 ctcgaggcga gcgctgacga gccggctcgt cttcagcagg gccgccagct cctcggcgcg
     4021 gtggctgctc tcctccagca gacgccgctg ctcgacggcc gcagcgagtg gctgcgcgag
     4081 ctccgcgacg atctcgaggt cggcgtcgtc gaaggcgccc ggcagccgtg ccgcgacggt
     4141 cacggcgccg gccaccgccc cgcgcaccgt gagcggcgcg agcacggccg accggtagcc
     4201 gcgcgccccg aacgccgcgc ggctcgcggc tggcacgtcc gcacccgcca ggtcgtcggc
     4261 acggagcggg gcgccgctgt ccaccacgcg cgcgagcagg gtgccggcga ggggcatccg
     4321 cacgtcgcgg cgcgcgccga agtgcagcga ccacgccggc acgtccacga tcgtgaagtc
     4381 accgcgctcg gcgtcgatga gcgagacgcc gacgccgtcg aagcggacga gcgcgccgag
     4441 tccctccccg aaggcgtcca ggaccgcctc cagcggctcg ctcgacgcgc ccagccgcgt
     4501 gatctcgctg agcacccgca tccgcagcgt ccgccggtgc gcctgccaga cccggtcgat
     4561 ggcagtgccc agatcgcgcg agaaccggac gggcagcgtc gggcgtccga gccggcgccc
     4621 gaggagcgtg agggccccca tcggcgcccc tggcgcgccg agcggggccc gcagcacgac
     4681 gtgacgtccg ggcagcgccg gcacggcgcc ccggctcacg cggcctccgc tgatcggccg
     4741 cgcggcctcc ccgcgcagcc acgcgtcgag gggcgggggc acccggccgc gcgcggcggt
     4801 gaccacgagt gcctccccgc gtggggggcg gaagacgagg gcgccggccg cggcgccggt
     4861 gacccccacc agacgccgga gcatccccgc gagcccgacc tcgatcggcg acacggcgct
     4921 gagctgcatc aagaggtcgg ggaagaccct tccgatcttc atcgtggcgg ggctcatgga
     4981 agccggagaa ctttccccgg caagctgccg gtgtgctcgc cgtcccggac gacgaagcgc
     5041 ccgttgacga gcacgtgatc gatgcccgcc gcgtagcggt ggggctcctg gtacgtcgcc
     5101 tggtcggcga cgcgccggga gtcgaagacg acgaggtccg cgcgcgcgcc cggccggatc
     5161 acgccgcggt ccttgagccc gaggcggcgc gccggcaggc ccgtcatctt gtgcacggcc
     5221 tcgggcagcg tgagcacgcg ctgctcgcgc gcgtactcgc caagcacgcg ggcgaaggtg
     5281 ccgtagctgc gggggtgggg cttgcccgtg cccagctcgc catacggggc gagtgacgag
     5341 ccatcggagc cgatcatgac ccgcggatgc gccagcgccg ccctgaggtc gcgctcgtcg
     5401 agctggaaga ggatcacgta cccgcggccg cgttcgtcgg cgatcagctc gagcgcggcg
     5461 tcgagcgaat cgacgccgcg ctcgcgggcg atctcgctca gccgccgtcc ctccgcatcc
     5521 gggcggtgag gggccgcggc caccatgacg ttctcccacc cgacccgcgc gacgaggctc
     5581 tggccgttgt ccggcgactc gacgtcgcgg cggatgcgcg cccgggccgt cgggtcgccg
     5641 aggcgggcca gcatcgcctc gatgccgccc tcgagcaccc agtcgggcag gagggtccgg
     5701 agcgtcgtgc tggacgccgt gtacgggtac acgtcggcca tgacgtcgag cccctccgcg
     5761 caccccgcgt cgatcagcgc cagggcctcg ggcaccttgc cccagttcgg gcggcccgcg
     5821 gccttcacgt ggctcacctg caccggcagg tccgcctccc cacccacgcg gatcgcctcg
     5881 cgcacggcgt cgagcagcgt cgcgccctcg ccgcggatgt ggctcgcgta gatgccgcgg
     5941 cctcgggcgg cgcgcgcgac ggccacgatc tcctccgtcg ccgcgaagga gccgggcgcg
     6001 tagatgagac cggtggacat gccccacgcg ccgtcctcga tcgcctggcc gagccagcgc
     6061 tgcatccgcg tcaactcgtc gccggtcgcc gggcggcgcg caaagcccat ggcggcgatg
     6121 cgcagcgtac cgtggccgac gagctggacg acgttgagcg ccgtgccctc ggcgtcgaag
     6181 gcgtcgaggt actcgcccac cgagcgccag gcgaagtcca gccgcttcgg cacgtgcagg
     6241 gcgaacgagc gcaggtcctc gaggtgctcc gtcgagactg gcgccggtga gaatccgcag
     6301 ttgccgacga cctccgtcgt cacgccctgg cggaccttcg actcggcgcg gcgattggcc
     6361 cacaggcgcc agtccgagtg ggagtgcatg tcgatgaagc ccggcaccag cacgcgcccg
     6421 gcgacgttga cggtccggcc ggcggcctcg cgcgagaggt cgcccacgac ggcgatcacg
     6481 tcgtcacgaa cgccgacgtc ggcccggctg ccggtggccc cggtcccgtc gatcaccgtc
     6541 gcgccctcga gcttgaggtc gagcatcgcg ggccggctca caggctcggc gccgaggtga
     6601 agcccagcac ctcttcgcag aactgcgcgg cgtcgagcag ccgctgctcc tcgcccgccg
     6661 cggcgacgag ctgcacggcg tgcggcaacc cgttgtcggc gagcccgctc ggcaaggtga
     6721 tggcgggcac gccggcccag gtccaggggg cgcagagcga cgcgtccccc gtccagccga
     6781 ggcccgccgg cgcgggcgcg ggcgccgtcg gcgcgagcag ggcgtcggcg gcggcgagca
     6841 gcggcagcac ttcgtcgcga aagcgcaacc gcgcgcggtt cgcgcgcacg tacgcggtcg
     6901 ccggcagggc cagcccggcc tgcacgagcg cgcccatctc gcggccgtac tccccgccgt
     6961 gcgcgcgcca ggattcctcg tgataggtcg ccgcctccgc ctcgagcacg aggcgtcccg
     7021 cctcggccag gtcggcgaac gaggcgggga gcttcatctc gacgaccgag gcgccggcgc
     7081 gcgccagacg atcggcgacc gcctggacct gcgcggctgt cgccggctcg cagcgagcga
     7141 gcagctctcc ggcgaccgcc agccgcggcg cccgcggggc gacggggtgc agctcccgcg
     7201 ccatgagcac gccgaggatg agggcggcgt cggccaccga ccgcgcgaag gccccgacgt
     7261 gatccagcga ccatgcgagc gggatgacgc cgtccacggg gatcgcgcca tgcgtgggct
     7321 tgacgcccac gacgccacag taggcggccg ggcgcaggac g
//
LOCUS       DATIAZ010000184        13852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1757098, whole genome shotgun sequence.
ACCESSION   DATIAZ010000184 DATIAZ010000000
VERSION     DATIAZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757098"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VLF19_06515"
     CDS             <1..271
                     /locus_tag="VLF19_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSE92941.1"
                     /translation="IRGVNSEGVLDNLLERRLLRIAGRKEAPGRPFLFETTREFLVAF
                     GLRDLADLPKVESELIVPDVPVPADGHAETEQDPGPGRAGVAPQG"
     gene            207..941
                     /locus_tag="VLF19_06520"
     CDS             207..941
                     /locus_tag="VLF19_06520"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSE92942.1"
                     /translation="MATLRLNKILAQAGLASRRKADELIAAGRVAVNGAVVREVGTLA
                     DPARDRVEVDGRLVPTAVPLRYLLLNKPRGVLTSRSDPRGRAVVTDLVPAGTRLYPVG
                     RLDADVEGALLLTNDGALTHRLLHPRYGLPRVYEAVVAGEVRRADVARWRRGVTLDDG
                     PAVPAAVDLVHASARLSRLRLTFTEGRKHEVKRYCEALGHPVVRLRRVAFGPIVLGEL
                     PPGRWRALSPAEVSALRRAAVAPAAR"
     gene            1020..2081
                     /gene="pheA"
                     /locus_tag="VLF19_06525"
     CDS             1020..2081
                     /gene="pheA"
                     /locus_tag="VLF19_06525"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSE92943.1"
                     /translation="MSLDDWRSRINDLDNQILSLLNQRAEAALKIGDLKRRQDQPAYA
                     PERETEILARLVTRNGGPLPADALRAIWREILSACRALERPLSVGFLGPRATFTHQAA
                     LQQFGAGVEYVAARSIVDVFDDVEQGRVDYGVVPVENTTEGAVNVTLDRLIDSDSVIC
                     GELMLPIAQHLLSRADELGDVKLVLSHPQGLAQCRRWLSEHLPDVPTQETTSTAAAAG
                     QAAADVTTAAIASDLAARLYDVPILRSRIEDNPHNSTRFLVIGRRGPGPSGRDKTSIL
                     FAMRNEPGVLHRILEPFARRGLNLTKIESRPAKRRPWEYVMFVDFEGHRATAAVASAL
                     HEIEDRTIFLKVLGSYPAG"
     gene            2090..3199
                     /gene="hisC"
                     /locus_tag="VLF19_06530"
     CDS             2090..3199
                     /gene="hisC"
                     /locus_tag="VLF19_06530"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSE92944.1"
                     /translation="MPTQWESLANEHILGIAPYEPGKPIEELERELGIADAIKLASNE
                     NPLAPSDRVQRAISAALRHLNRYPDGSGFYLRQALAKKHGVSPDQVLLGNGSNELIEL
                     LARAFLKPGDEAVVPHPSFVVYPMIVQAAGGIRVMVMLKDYRLDLEAMARAITPLTKI
                     VFIANPNNPTGTIVTAAEVEHFMARVPERTIVVFDEAYVEFALGPDFPDTLSLVKQGR
                     KVLVLRTFSKASSLAGLRVGYAVAEADAIALMNRIRQPFNVNSLGQAAALAALDDEPH
                     VLECVRMIEAGRHYLYDEFKALGLKYVPSRANFILVDVGRSAAEIYQKLLHEGVIVRP
                     MTPFGMESTLRITVGTPEENRRLVKGLRAVLGKPV"
     gene            3199..4050
                     /locus_tag="VLF19_06535"
     CDS             3199..4050
                     /locus_tag="VLF19_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HSE92945.1"
                     /translation="MLRRLAVVGVGLLGGSVAKAARAHGLAREILGVGRDAGRLRPAL
                     ADGTLDRATTDLATGVGDADLVVLAAPVRTIETLLPAVWRAAAEDVVVTDVGSTKGAI
                     VAAAQRLAAERPLAFVGSHPMAGSERSGYGVARADLFRGATVIVTPAEPSPPAAVKTV
                     TTFWEGTGAGRVLTLDPDAHDRAVAAISHLPHLVAFALMDGVTRFDAAALDVAARGFR
                     DTTRIAASDPAVWEEIFLANREALGASVRAFERALTDLVRLVEAGDAPALRAALARIK
                     VEREGLA"
     gene            4047..5348
                     /gene="aroA"
                     /locus_tag="VLF19_06540"
     CDS             4047..5348
                     /gene="aroA"
                     /locus_tag="VLF19_06540"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSE92946.1"
                     /translation="MRLRVRPVRRLAGRVEVPGDKSISHRAALLGALADGVTEIRGLL
                     EAEDCLRTIAAVEALGAEVTRKGPGEYRVSGTGELQEPGDVVDCGNSGTTARLLMGVL
                     AGQPFWTCMTGDASLRRRPMARVTEPLRRMGATITGRADGGRLPLAVRGARPLRAIAY
                     EAPVASAQVKSAVLLAGLHADGAVAVTEPAQSRDHSERMLRQFGARVETRGLTTTITP
                     GALRGTSVVVPGDISSAAFLLVAGIVVPDARVTVTGIGLNPTRTGVLEALEAMGARLD
                     VAPGESAGEPIGAVTAVTTELHGATVAGELVPRLIDEVPVLAVAAVLARGTTQIREAS
                     ELRVKESDRIAALARELGAMGADVRELPDGLALVGPARLRGARVASSGDHRIAMALAV
                     AGLVAQGETVVEDVACIATSFPQFADVLNALAGAEVAQVEE"
     gene            5372..6025
                     /gene="cmk"
                     /locus_tag="VLF19_06545"
     CDS             5372..6025
                     /gene="cmk"
                     /locus_tag="VLF19_06545"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSE92947.1"
                     /translation="MAIDGPAGAGKSTVARALARRLGYRLIDTGAMYRALAWSVDRAG
                     LPAEDGSALRRHLAAVEVTLDGEAVYVDGRDVSGEIRTPAISELTSRLTMLAPVRDKV
                     TPLQRRLAAAGGVVLEGRDTGTVVCPDADVKFFLDATVEERARRRQAELRERGVDCDL
                     TAVQADIQARDVQDTTRALAPLRKAADAIVIDTTGLSLEQVTESMLGEIKRRCSTRS"
     gene            6007..6732
                     /locus_tag="VLF19_06550"
     CDS             6007..6732
                     /locus_tag="VLF19_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSE92948.1"
                     /translation="MLYAILKSIVATLARLYCRLETRGTDHVPLPGPVLLVANHSSVL
                     DPPLVGAAAPRPVSFMAKAELFDVPLLGRLIHKLNARPVRREGGDAAALRTALRRLES
                     GEVLLVFPEGTRGPEGTLRPAKAGAGMLAVASGATVVPVYISGSGRSWPRGARLPRPT
                     KIMVRFGPPMRFGTGDRAARKESYEAASREMMAAIARLKDAAGSSGPGASPRDTSRGV
                     GEVGVGGASISPKYIQGRNGQHG"
     gene            6725..8455
                     /locus_tag="VLF19_06555"
     CDS             6725..8455
                     /locus_tag="VLF19_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSE92949.1"
                     /translation="MAKNEPGERLGGQRKEGAYGAAEERMEDWYGSAGTGITEFEEGE
                     VVRGRVVHVGSSEVLVDVGYKSEGAIPIEEFHQAGALPKVGDELDVYLEAKEDAEGLI
                     VLSKEKADKIKVWDLVSQAYDKGTPLEGRVVEVVKGGLAVDVGVKAFLPGSQVDLRPV
                     KNLAGMVGQQIRAKVIKLNRRRGNVVLSRRVVMEEEREEKKKHTLEVLHEGLVLTGTV
                     KNITDYGAFIDLGGIDGLLHVTDMSWGRVGHPSEIFQVGDQVEVVVLHFDRETGRVSL
                     GYKQKSSDPWEHVEQKYAVGTKARGRVVSLTNYGAFIELEPGVEGLVHVSEMSWTRRV
                     RHPSKLVNVGDEVEVIVLDVNRGAKRISLGMKQVEPDPWQTIEERYTVGGRVTGRVRN
                     LTDFGAFVELEPGVDGLLHISDMSWTRSVGHPSEVLKKGQEIETQILNIDQDNKRISL
                     GLKQIQPDPWSTVAQRYPMGSRVTGKVVRLTDFGAFVELEPGVDGLLHISQMSNRPIS
                     RPDEIVNVGDEIGLLVIRVDPHERRIGLSLKELAHAVDFGGGPEERSQGQGPRRGKQR
                     RRDDYDDDEE"
     gene            8465..9370
                     /gene="sppA"
                     /locus_tag="VLF19_06560"
     CDS             8465..9370
                     /gene="sppA"
                     /locus_tag="VLF19_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSE92950.1"
                     /translation="MGRRLAVVGLTVAVWLAVLAVFLIAVSAALRGPEAGGVGFGPRV
                     AVVELDGIILDVEELLRDLKTHRENPLVRAVVIRINSPGGVVGPTQELHDALLRLRKA
                     GKPVVASLGAVAASGGYYAAVAADQIFANPGTLTGSIGVIMQLANLEGLMKKVGVGYV
                     VVKAGEFKDIGNVSRPMTAEERRVLQMLLDDVHGQFIGAVAAGRKLERADVARFADGR
                     IFSGVQAKGLGMVDELGGLEQAVERAATLAGLPLPPRVIPPRRRFSLFDLVRNQVSLG
                     LVTGVGSRLLTPALPVFKTPLYLMD"
     gene            9577..9852
                     /locus_tag="VLF19_06565"
     CDS             9577..9852
                     /locus_tag="VLF19_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HSE92951.1"
                     /translation="MTKAELIDEVSKVANLTKKETETIVNTVFDNITDALAKGDKVEL
                     RGFGSFRIRHRNSRKGRNPKTGSAVDVPEKRVPFFKVGKRLRELVNT"
     gene            9869..10348
                     /locus_tag="VLF19_06570"
     CDS             9869..10348
                     /locus_tag="VLF19_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HSE92952.1"
                     /translation="MKRLWAPWRMAYVGGTEATAGCIFCHALVEADDRRALVLARGAR
                     AFLILNAYPYAVGHVMAALNRHVASVAEADVEDLADAMRLVGLATRALAEEYRAQGFN
                     IGVNQGRIAGAGIEDHLHVHVVPRWPGDANFMPVLGEVKVLPEALETTWERLKGRFA"
     gene            10345..12903
                     /gene="mutS"
                     /locus_tag="VLF19_06575"
     CDS             10345..12903
                     /gene="mutS"
                     /locus_tag="VLF19_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HSE92953.1"
                     /translation="MTGPAGALTPMMQQYRELKRRFPDYLLLFRLGDFYELFFEDAAD
                     AVGLLGITLTSRQGAPMAGIPHHAADGYVARLVRAGRKIALCEQLEAPGQGKKLLKRD
                     VVRLITPGTVTDTAYLATGANNFLLAVAPGREGTGLALLDVSTGDFWVGEQTGTGPDL
                     LESALMRRPAEIVVPEPLRPSALVARLQATGATLTFWDPAAFVPRRAAADLAAHFGTA
                     ALGAFGVGDMTVGLGAAGAALAYVRATQGDRLGHLTRLTRFATGEAMTLDATAVETLE
                     LCESRAGDARSSLYGVLDETVTPMGGRLLRQWLLRPLLDPAAIAERQDAVAALVEAPA
                     ARAQLRALLRRVGDLERLTSRATLGVAHARDLVGLRACLAPLDDLRAALHGLGPRTLA
                     ETREDVRALPSLRALLQDALVDEPPLTLHEGGLIREAWSEPLRAIVEDARHAREWIGG
                     LEARERLRTGIAGLRVRFNRVFGYGIEVTHAHTARVPPEYVRRQTLTGAERYVTPELK
                     EHEAKVLGADERRRRLEYELFDEVRRRVAEHAADLLTTARALARLDVLGALAEVAHER
                     GHTRPVVDRGDALVIVDGRHPVLEARAGDPVTPNDLALDAQARIVILTGPNMSGKSVY
                     LRQTGHIVILAQIGAWVPAREARIGVVDRVFTRVGAQDNLGRGQSTFLVEMVETASIL
                     NNVTPRSLVLLDEVGRGTSTFDGLAIAWAVVEALQAGAGPKVLFATHFHELTQLARRF
                     PAVCNFHVAVREWNDEIIFLHKVRAGGTDRSYGIQVARLAGLPEPVIARAKALLAELE
                     SAGQSRADARDATQLGLFAPPPDPIAEELAVLDLGHLTPIEALNLLAKWQAQRR"
     gene            12900..>13852
                     /gene="mutL"
                     /locus_tag="VLF19_06580"
     CDS             12900..>13852
                     /gene="mutL"
                     /locus_tag="VLF19_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSE92954.1"
                     /translation="MRIRKLPDHLVNKIAAGEVVERPASVVKELVENSLDADARLVCV
                     DVKDGGAGLVRVADDGTGMTAAELTLALERHATSKLARDEDLEAIATLGFRGEALPAI
                     CAVSRFAITTHARTEDEGVRVAGAGGVIAQRLAVPAEGGTTVEVADLFFNTPARLKFL
                     KSPTTELAASLRLLTQLAIAHPGTQFRATSNGRPVLTAPRAGDLRARVGALWGWDIAG
                     RLVDVARAEHGVEVRGVASPPDLTRGGRDDVVVVVNGRPVRDPALFVAVVHAYRPLLA
                     RDRFPLVALDVRLPLADVDVNVHPTKAWVRFRHPRLVQEMVV"
BASE COUNT         1969 a         4957 c         4982 g         1944 t
ORIGIN      
        1 catccgcggc gtcaactccg agggcgtgct cgacaacctc ctcgagcgac ggctcctccg
       61 catcgccggg cgcaaggagg cgccgggccg gccgttcctg ttcgagacga cgcgggagtt
      121 tctcgtggcg ttcgggttgc gtgacctcgc cgacctgccg aaggtcgaga gcgagctgat
      181 cgtgcccgac gtgcccgtcc ccgccgatgg ccacgctgag actgaacaag atcctggccc
      241 aggcagggct ggcgtcgcgc cgcaaggctg acgagctcat cgccgccggc cgcgtcgccg
      301 tcaacggtgc cgtggtccgt gaggtcggca cgctcgccga cccggcccgc gaccgcgtcg
      361 aggtcgacgg gcgtctcgtc cccaccgccg tacccctccg ctacctgctg ctcaacaagc
      421 cgcggggcgt gctgacgtcg cgcagcgacc cgcggggccg cgcggtcgtc accgacctgg
      481 tgcccgccgg tacgcggctg tatccggtgg ggcgcctgga tgccgacgta gagggtgccc
      541 tgctcctgac caacgacggc gcgctgaccc atcggctgct ccacccgcgc tacgggttgc
      601 cacgcgtgta cgaggccgtc gtcgcgggtg aggtccggcg cgcggacgtc gcgcggtggc
      661 gccggggcgt gacgctcgac gacggaccgg cggtgccggc ggccgtcgac ctggtccacg
      721 cgagcgcgcg cctgagccgg ttgcggctca cgttcaccga gggccgcaaa cacgaagtga
      781 agcgatactg cgaggcgctc gggcatcccg tcgtccgcct ccggcgcgtc gccttcgggc
      841 cgatcgtcct cggcgagttg ccgccgggcc ggtggcgagc gctgagcccg gcggaggtga
      901 gcgcgctgcg ccgcgccgcc gtcgcgccgg ccgcgcgctg aggccgcggc gcgcaacttg
      961 cgctcgttct cgccggtagc ctataatcca ccaattcagc agagcaggca ggtgtcctca
     1021 tgagcctgga cgattggcgc tccaggatca acgacctcga caaccaaatc ctcagcctcc
     1081 tcaaccagcg cgccgaggcc gccctcaaga tcggagacct caaacgtcgg caggatcagc
     1141 cggcgtacgc gcccgagcgg gaaacggaga tcctcgcccg cctcgtgacg cgcaacggcg
     1201 gaccgctccc cgccgacgcg ctgcgcgcca tctggcgcga gatcctctcc gcgtgtcggg
     1261 cgctggagcg cccgctctcg gtcggcttcc tcggtccccg ggccaccttc acccaccagg
     1321 ccgccctgca gcagttcggc gccggcgtcg agtacgtggc ggcacgctcc atcgtggacg
     1381 tcttcgacga cgtcgagcag gggcgggtgg actacggcgt ggtgcccgtg gagaacacga
     1441 ccgagggcgc cgtcaacgtg acgctggatc ggctcatcga ctccgactcc gtgatctgcg
     1501 gcgagctgat gctgcccatc gcccagcacc tgctctcgcg ggcggacgag ctcggcgacg
     1561 tgaagctcgt cctctcccat ccccagggcc tggcccagtg ccgccggtgg ctgagcgagc
     1621 acctgccgga cgtgccgacc caggagacga cgtccacggc ggcggcggcg ggacaggctg
     1681 ccgccgacgt cacgacggcg gccatcgcct ccgacctggc ggcgcgtctg tacgacgtcc
     1741 ccatcttgag gtcgcggatc gaggacaacc cccacaactc gacgcgcttt ctcgtcattg
     1801 gccggcgggg ccccggtccc agcgggcgtg acaagacctc catcctcttc gccatgcgca
     1861 acgagccggg cgtgctccac cgcatcctgg agccgttcgc gcgccgcggc ctgaatctca
     1921 ccaagatcga gtcgcgaccg gcgaagcgcc ggccgtggga gtacgtgatg ttcgtcgact
     1981 tcgaggggca tcgcgcgacc gcggccgtcg cgtcggcgct gcacgaaatc gaggaccgga
     2041 ccatcttcct caaggtcctc ggctcgtacc ccgcgggata ggagaggaca tgcccactca
     2101 gtgggagtcg ctggccaacg agcacatcct gggcatcgcg ccctatgagc ccggcaagcc
     2161 gatcgaggag ctggagcggg agctgggaat cgccgacgcc atcaagctcg cgtccaacga
     2221 gaatccgctc gccccgtccg accgcgtgca acgggcgatc tccgccgcgc tgcgccacct
     2281 caaccgctat cctgacggca gcggcttcta cctgcgccag gcgctggcca agaagcacgg
     2341 cgtgtcgccc gaccaggtgc tgctgggcaa cggctcgaac gagctgatcg agctcctggc
     2401 gcgggccttc ctcaagcccg gcgacgaggc cgtggtcccg cacccctcgt tcgtcgtgta
     2461 ccccatgatc gtccaggccg cgggcggcat ccgcgtgatg gtcatgctga aggactaccg
     2521 cctcgacctg gaggcgatgg cccgcgcgat cacgccgctc accaagatcg tgttcatcgc
     2581 caacccgaac aaccccacgg gcacgatcgt caccgccgcc gaggtggagc acttcatggc
     2641 gcgcgtgccc gagcggacga tcgtcgtgtt cgacgaggcg tacgtggagt tcgcgctcgg
     2701 cccggacttc cccgacacgc tgtcgctggt caagcagggg cgcaaggtcc tcgtcctgcg
     2761 cacgttctcc aaggccagca gcctcgccgg gctccgcgtc ggctatgcgg tggccgaggc
     2821 cgacgccatt gccctcatga accgcatccg gcagcccttc aacgtcaact ccctggggca
     2881 ggccgccgcg ctcgccgcgc tcgacgacga gccccacgtg ctcgagtgcg tgcgcatgat
     2941 cgaggccggc cgccactacc tctacgacga gttcaaggcg ctggggctca agtacgtccc
     3001 ctcccgcgcg aacttcatcc tggtcgacgt cgggcgcagc gcggcggaga tctaccagaa
     3061 gctgttgcac gagggtgtca tcgtccgccc gatgacgccc ttcgggatgg agagcacgct
     3121 gcgaatcacg gtgggcacgc cggaggagaa ccgccggctc gtcaaggggc tgcgcgccgt
     3181 gctcgggaaa cccgtctagt gctccggcgg ctcgccgtcg tgggcgtggg actgctgggc
     3241 ggctccgtgg ccaaggccgc acgggcccac gggctggccc gcgagatcct cggcgtcggc
     3301 cgcgacgccg ggcgcctgcg cccggcgctg gccgacggca cgctggaccg cgcaacgacg
     3361 gacctggcca ccggggtcgg tgacgccgac ctcgtcgtcc tcgcggcgcc ggtgcggacg
     3421 atcgagacgc tgctgccggc cgtgtggcgc gccgccgccg aggacgtcgt cgtcaccgac
     3481 gtcggctcca ccaagggggc catcgtcgcc gccgcccagc ggctcgcggc cgagcgcccg
     3541 ctcgcgttcg tgggcagcca cccgatggcc ggctccgagc gcagcggcta cggagtggcg
     3601 cgcgccgatc tcttccgcgg cgcgacggtc atcgtcacgc ccgccgagcc gagccccccg
     3661 gcggcggtga agacggtcac caccttctgg gaggggaccg gcgcgggccg cgtcctcacc
     3721 ctcgaccccg acgcccacga ccgcgcggtg gcggcgatca gtcacctgcc gcatctcgtc
     3781 gcgttcgcgc tgatggacgg ggtcacccgc ttcgacgccg ccgcgctgga cgtggccgcg
     3841 cgtggctttc gcgacacgac ccgcatcgcg gcctccgacc ccgcggtgtg ggaggagatc
     3901 ttcctcgcca accgggaggc gctcggcgcg agcgtccgcg cgttcgagcg ggcgctcaca
     3961 gacctcgtgc ggctcgtcga agccggtgac gcgccggccc tccgagcggc actggcgcgg
     4021 atcaaggtcg agcgcgaggg cctcgcgtga ggctccgcgt gcggccggtg cggcgcctcg
     4081 ccgggcgcgt cgaggtgccc ggcgacaagt ccatctccca ccgggcggca ctgctgggcg
     4141 cgctcgccga cggcgtgacg gagatccgcg gcctgctgga ggccgaggac tgcctgcgga
     4201 cgatcgccgc ggtggaagcc ctgggcgcgg aggtgacacg caagggaccg ggcgagtacc
     4261 gcgtctcggg caccggggag ctccaggagc ccggcgacgt cgtcgattgc ggcaactcgg
     4321 gcacgacggc gcgactgctg atgggagtac tggcgggcca gccgttctgg acgtgcatga
     4381 ccggtgatgc ctcgctgcgt cgccgcccca tggcacgtgt caccgagccg ctgcgccgga
     4441 tgggcgcgac gatcacgggc cgcgccgacg gcggccgcct gccgctggcc gtgcgcggtg
     4501 cgcggccgct gcgcgcgatc gcctacgagg cgccggtcgc ctccgcgcag gtcaagtcgg
     4561 cggtcctcct cgccgggctc cacgcggacg gcgcggtcgc cgtcaccgag cccgcgcagt
     4621 cacgcgacca ctccgagcgg atgctgcggc agttcggcgc ccgcgtggag acgcgcgggc
     4681 tcaccaccac gatcacgccc ggcgcgctcc gggggaccag cgtcgtggtg cccggtgaca
     4741 tctcctcggc ggcattcctg ctcgtcgccg ggatcgtcgt ccccgacgcg cgcgtcaccg
     4801 tcaccggcat cgggctcaat cccacgcgga cgggcgtgct ggaggcgctc gaggccatgg
     4861 gggcccgtct cgacgtggcg cccggggaga gcgcgggcga gccgatcggt gcggtgacgg
     4921 ccgtcacaac ggagcttcac ggcgccaccg tcgccgggga gctcgtgcca cggctcatcg
     4981 acgaggtgcc ggtgctcgcg gtggcggccg tgctcgcgcg gggaacgacg cagatccggg
     5041 aggcgtcgga gctgcgcgtc aaggagtcgg atcgcatcgc agcgctggcc cgcgagctcg
     5101 gcgccatggg cgccgacgtg cgcgagttgc ccgacggcct ggcgctcgtg ggaccggcgc
     5161 gcctgcgggg ggcgcgcgtg gcgagcagcg gcgatcaccg gatcgccatg gccctcgccg
     5221 tcgccgggct ggtggcccag ggagagacgg tcgtcgagga cgtcgcgtgc atcgccacgt
     5281 cgtttccgca gttcgccgac gtgctcaacg ccctcgccgg cgccgaggtc gcgcaggtcg
     5341 aggaatgacg gcgcgggatc gcgggctcgt ggtggcaatc gacggcccgg ccggggccgg
     5401 caagagcacc gtcgctcgcg cgctggcccg gcggctcggc taccggctga tcgacacggg
     5461 tgccatgtat cgggcgctgg cgtggagcgt ggaccgcgcg gggttgccgg ccgaggacgg
     5521 ctcggcgctg cggcgtcacc tggccgccgt cgaggtcacg ctcgacgggg aagccgtcta
     5581 cgtcgacggg cgggacgtgt ccggcgagat ccggacgccg gccatcagcg agctgacgtc
     5641 gcggctcacc atgctcgcgc ccgtacgcga caaggtcacg ccgctccagc ggcggctggc
     5701 cgcggccgga ggcgtggtgc tggagggccg ggacaccggc accgtcgtgt gtcccgacgc
     5761 cgacgtgaag ttctttctgg acgccacggt ggaggagcgc gcgcgccggc ggcaggccga
     5821 gctacgcgag cgcggcgtgg actgcgacct gacggccgtg caggccgaca tccaggcccg
     5881 cgatgtgcag gacaccacgc gtgcgctcgc gccgctccgc aaggcggcgg acgccatcgt
     5941 gatcgacacg accgggctca gcctggagca ggtgaccgag agcatgctgg gggagatcaa
     6001 gcgccgatgc tctacgcgat cctgaagtcc atcgtggcga ccctggcacg gctctactgc
     6061 cggctggaga cccgcggcac ggaccacgtg ccgctgccgg gcccggtgct gctcgtcgcc
     6121 aatcactcga gcgtcctgga tccgccgctc gtgggcgcgg cggcgccgcg cccggtgtcg
     6181 ttcatggcga aggcggagct gttcgacgtt ccactcctcg gccgcctcat ccacaagctc
     6241 aacgcgcgtc cggttcgccg ggaaggcggt gacgcggccg cgttgcggac ggcccttcgc
     6301 cggctcgagt cgggagaggt cctgctggtg ttcccggagg gcacccgagg ccccgaggga
     6361 accctgcggc cggcgaaggc cggggcgggc atgctcgccg tggcaagcgg agcgaccgtg
     6421 gttcctgtat atatcagtgg ctcggggcga tcgtggccca ggggggcccg gctgccacgt
     6481 cccacgaaga tcatggttag gttcggccct ccgatgcggt tcggcaccgg cgacagggcc
     6541 gccaggaagg agtcttacga ggccgccagc cgggagatga tggcggccat cgcgcgcctc
     6601 aaggatgcgg ccgggtcctc cgggcccggg gcgtcgccgc gtgacacgag tcgaggagtc
     6661 ggcgaggtgg gtgtgggagg agcctcgatc tcgcccaaat acattcaggg gaggaatggg
     6721 cagcatggct aagaatgaac cgggcgaacg cctcggcggc cagagaaagg agggcgccta
     6781 cggcgcggcc gaggaacgga tggaagactg gtacggcagc gccggcaccg gcatcaccga
     6841 gttcgaggag ggcgaggtgg tccgcgggcg ggtggtgcac gtcgggagca gcgaggtcct
     6901 cgtggacgtc ggctacaaga gcgagggcgc gatcccgatc gaggaattcc accaggcggg
     6961 cgcgctgccg aaggtcggcg acgagctcga cgtgtacctc gaggccaagg aggacgccga
     7021 agggctgatc gtcctctcca aggagaaggc cgacaagatc aaggtctggg atctcgtctc
     7081 ccaggcctac gacaagggca cgcccctcga ggggcgcgtc gtggaggtcg tcaagggcgg
     7141 cctcgccgtc gacgtcgggg tcaaggcgtt cctgcccggg tcccaggtgg acctccggcc
     7201 ggtcaagaac ctcgccggca tggtcggcca gcagatccgc gccaaggtca tcaagctgaa
     7261 ccgccggcgc ggcaacgtgg tcctctctcg ccgggtcgtg atggaggagg agcgcgagga
     7321 gaagaagaag cacaccctcg aggtcctgca cgagggcctt gtgctgacgg gcacggtcaa
     7381 gaacatcacg gactacggtg ccttcatcga cctcggcggc atcgacggac tgctacacgt
     7441 caccgacatg agctggggcc gggtcgggca tccgtcggaa atcttccagg tcggcgatca
     7501 ggtcgaggtg gtcgtgctcc atttcgaccg cgagaccggc cgggtgtcgc tcggctacaa
     7561 gcagaagtcg tccgatccct gggagcacgt cgagcagaag tacgccgtcg gcaccaaggc
     7621 gcgcggccgg gtcgtgagtc tcaccaacta cggcgcgttc atcgagctgg agcccggcgt
     7681 ggagggcctc gtgcacgtct cggagatgtc ctggacgcgc cgcgtgcgcc atccctcgaa
     7741 gctcgtcaac gtcggcgacg aggtcgaggt gatcgtgctc gacgtcaatc gcggcgccaa
     7801 gcggatctcc ctgggcatga agcaggtcga gcccgacccc tggcagacca tcgaggagcg
     7861 ctacacggtc ggcggccgtg tgaccggccg cgtgcgcaac ctcaccgact tcggcgcgtt
     7921 cgtggaactg gagcccggcg tcgacggcct cctgcacatc tccgacatgt cctggacgcg
     7981 ctcggtgggg catccctcgg aggtgctcaa gaaaggccag gagatcgaga cgcagatcct
     8041 caacatcgac caggacaaca agcgcatctc gctcggtctc aagcagatcc agcccgatcc
     8101 gtggtcgacg gtggcccagc gttacccgat gggctcacgg gtcacgggca aggtcgtgcg
     8161 gctgaccgac ttcggcgcgt tcgtggagct ggagcccggt gtcgacggcc tcctgcacat
     8221 ctcccagatg tccaaccgtc ccatcagccg ccccgacgag atcgtcaacg tcggtgacga
     8281 gatcgggctc ctggtcattc gcgtcgatcc ccacgagcgc cgcatcgggc tctcgctgaa
     8341 ggagctcgcc cacgccgtgg acttcggcgg cgggcccgag gaacgtagcc aggggcaggg
     8401 cccgcgccgt ggcaagcagc gccggcgcga cgactacgac gacgacgagg agtagaggct
     8461 gagggtgggc cgtcgcctcg ccgtcgtcgg gctcaccgtg gccgtctggc tggccgtgct
     8521 cgccgtgttc ctgatcgcgg tgtcggcggc gctccgtggt ccggaggcgg gcggcgtcgg
     8581 cttcgggccg cgcgtggcgg tggtcgagct ggacggcatc atcctcgacg tcgaggagct
     8641 gctgcgcgat ctgaagaccc accgcgagaa cccgctcgtg cgcgcggtgg tgatccgcat
     8701 caacagtccc ggcggcgtcg tggggcctac ccaggagctg cacgacgcgc ttctccgcct
     8761 gcggaaggcc ggcaagccgg tcgtcgcctc gctcggcgcc gtcgctgctt cgggcggcta
     8821 ctacgccgcc gtggccgccg accagatctt cgccaatccg gggacgctca cgggctcgat
     8881 cggggtcatc atgcagctgg cgaacctcga ggggctgatg aagaaagtcg gcgtcggcta
     8941 cgtggtcgtc aaggccgggg agttcaagga catcggcaac gtgtcgcgac cgatgaccgc
     9001 cgaggagcgg cgcgtcttgc agatgctgct cgacgacgtc cacgggcaat tcatcggcgc
     9061 cgtggccgcc ggccgcaagc tcgagcgcgc cgatgtcgcg cggttcgccg acgggcgcat
     9121 cttctcgggc gtccaggcca aggggctcgg catggtcgac gagctcggtg gtctcgagca
     9181 agcggtcgag cgagccgcga cgctcgcagg actgcccttg cctccgcggg tcatcccgcc
     9241 gcgccggcgt ttctcgctct tcgacctggt gcgtaaccag gtgagcctgg gcctcgtcac
     9301 cggtgtgggg agccgcctgc tgacacccgc actacccgtc ttcaagacgc cgctctatct
     9361 gatggactga cgaggatccc gccagcttaa cctccagtga tcacggatgc gatgtgaccc
     9421 aagctactgt ttctacgcca atatgactca cgaatcatcg ggtccccagc ggcgatgcag
     9481 aggctaaccc cttatgagag ttgacattct cgatttccac gtgctacggt cgatcgagct
     9541 tccgggcagg tccccgggtg tcggaggggt ggggctatga cgaaagccga gttgatcgac
     9601 gaggtctcga aggtcgccaa cctgacgaag aaggagaccg agacgatcgt caacaccgtc
     9661 ttcgacaaca tcaccgatgc gctcgcaaag ggcgacaagg tcgagctgag aggcttcggc
     9721 agcttccgca tccgccatcg gaactcccgc aaggggcgca acccgaagac tggcagcgcc
     9781 gtagacgtcc ccgagaaacg cgtcccattc ttcaaggtcg gcaagcgcct gcgcgagctc
     9841 gtcaacacgt gaggccgcgc ccggccgcgt gaagcgcctc tgggcgccgt ggcggatggc
     9901 ctacgtcggt gggaccgagg ccacggccgg atgcatcttc tgccacgccc tcgtcgaagc
     9961 cgatgaccgt cgtgcgctgg tcctcgcgcg cggggcccgc gcgttcctga tcctcaacgc
    10021 gtatccctac gctgtggggc acgtcatggc cgccctgaac cgccacgtcg catccgtcgc
    10081 cgaggcggac gtcgaggatc tggccgacgc gatgcggctg gtaggcctcg ccacgcgtgc
    10141 gctcgcggag gagtaccggg cgcagggctt caacatcggc gtcaaccagg gccgcatcgc
    10201 cggggccggt atcgaggacc acctgcacgt ccacgtcgtc ccgcgatggc ccggagacgc
    10261 gaacttcatg cccgtgctcg gcgaggtcaa ggtcctgccg gaggcgctgg agacgacctg
    10321 ggagcgcctc aagggacgct tcgcgtgacg gggccggccg gcgcgctcac gccgatgatg
    10381 cagcagtacc gggagctcaa gcgccgcttt ccggactacc tcttgctctt ccgcctcgga
    10441 gatttctacg agctgttctt cgaggatgcc gccgacgccg tggggctgct cggcatcacg
    10501 ctcacgtcgc gtcagggcgc tcccatggcg ggcatcccgc accacgccgc cgacggctac
    10561 gtcgcgcggc tcgtgcgtgc cgggcgcaag atcgccctct gcgagcagct cgaggccccg
    10621 ggccagggca agaagctcct caagcgcgac gtggtccgcc tcatcacgcc gggcacggtg
    10681 accgacaccg cgtacctcgc gaccggcgcc aacaacttcc tgctcgccgt cgcccctggc
    10741 cgcgagggca ccgggctcgc cctcctggac gtgtccaccg gcgacttctg ggtgggcgag
    10801 cagaccggca ccgggccgga cctgctcgaa tccgcgctga tgcgccgccc ggcggagatc
    10861 gtcgtccccg agccgctccg cccgtcggcg ctggtcgcac gcctgcaggc gacgggggcg
    10921 acgctgacgt tctgggaccc cgccgcgttc gtgccgcgac gggccgccgc cgacctcgcc
    10981 gcgcacttcg ggacggccgc gctgggggcg ttcggtgtcg gcgacatgac cgtggggctc
    11041 ggcgccgcgg gcgccgcgct cgcctacgtg cgcgccacgc agggcgaccg cctcggccac
    11101 ctcacgcgcc tcacgcgctt cgccaccggc gaggcgatga cactcgacgc gacggccgtc
    11161 gagacgctcg agctctgcga gtcgcgcgcg ggcgacgcgc ggagctcgct ctacggcgtt
    11221 ctcgacgaga cggtgacgcc gatggggggt cggctcctgc gccagtggtt gctgcgcccc
    11281 ctcctcgacc ccgccgccat cgccgagcgt caggacgcgg tcgcggcgct cgtggaggct
    11341 ccggccgcgc gcgcacagct ccgggcgctg ctccggcgcg tgggcgacct cgagcgcctg
    11401 acgagccgcg ccacgctggg tgtcgcgcac gcccgcgacc tcgtcgggct gcgcgcgtgc
    11461 ctggcgccgc tcgacgacct ccgcgcggcc ctgcacgggc tcggcccgcg gacgctcgcc
    11521 gagacgcgcg aggacgtccg tgcgctgccg tccctccgcg cactgctcca ggacgcgctc
    11581 gtggacgagc cgccgctgac cctgcacgag ggcggactca tccgcgaggc gtggtccgag
    11641 ccgctccgcg cgatcgtgga ggacgcgcgg cacgcgcggg agtggatcgg cggcctcgag
    11701 gcgcgggagc gcctccgcac gggcatcgcg ggcctgcgcg tccgcttcaa ccgcgtgttc
    11761 ggctacggca tcgaggtcac ccacgcccac acggcccggg tcccgcccga gtacgtccgc
    11821 cgccagacgc tcaccggggc cgagcgctac gtgacgccgg agctcaagga gcacgaggcc
    11881 aaggtgctcg gtgccgacga gcgccggcgc cggctcgagt acgagctgtt cgacgaggtc
    11941 cgccggcgcg tcgccgagca cgcggccgat ctgctcacca cggcgcgcgc gctggcgcgc
    12001 ctggacgtgc tgggggcgct cgccgaggtc gcccacgagc gcggccacac ccgccccgtc
    12061 gtcgaccgcg gcgacgcgct cgtcatcgtg gacgggcggc atccggtgct cgaggctcgc
    12121 gcaggcgatc ccgtcacgcc gaacgacctg gcgctcgacg cccaggcgcg catcgtcatc
    12181 ctcaccggcc cgaacatgtc cggcaagtcg gtctacctcc gccagaccgg tcacatcgtg
    12241 atcctcgccc agatcggcgc ctgggtcccg gcccgcgagg cccgcatcgg cgtggtcgat
    12301 cgcgtcttca cgcgcgtggg ggcgcaggac aacctcggcc gcgggcagag cacgttcctg
    12361 gtcgagatgg tcgagacggc cagcatcctg aacaacgtca cgccgcggag cctcgtgctg
    12421 ctggacgagg tagggcgggg gacatccacc ttcgacgggc tggccatcgc ctgggccgtg
    12481 gtggaggccc tgcaggcggg cgcgggcccg aaggtgctct tcgccaccca cttccacgag
    12541 ctgacgcagc tggcccggcg attcccggcc gtgtgcaact tccacgtggc cgtgcgcgag
    12601 tggaacgacg agatcatctt cctgcacaag gtccgcgccg gcggcacgga ccgctcctac
    12661 ggcatccagg tggcgcgcct ggctggccta cccgagcccg tgatcgcccg ggccaaggcg
    12721 ctgctcgccg agctcgagtc cgcgggccag agccgggccg acgcgcgaga cgcgacgcaa
    12781 ctggggctgt tcgcgccccc gcccgatccc atcgccgagg agctggcggt gctcgacctg
    12841 ggccacctca caccgatcga ggccctgaac ctgctggcca agtggcaggc gcagcggcga
    12901 tgaggatccg gaagctgccg gatcatctgg tcaacaagat cgccgccggc gaggtggtgg
    12961 agcgcccggc ctcggtggtc aaggagctcg tcgagaactc cctcgacgcc gacgcacggc
    13021 tcgtgtgcgt cgacgtgaag gacggcggcg ccggcctcgt gcgcgtcgcg gacgacggca
    13081 ccggcatgac ggccgcagag ctgacgctcg cgctcgagcg tcacgcgacc tccaagctcg
    13141 cccgcgacga ggatctcgag gccatcgcca cgctgggttt ccgcggcgag gcgctacccg
    13201 cgatctgcgc ggtgtcgcga ttcgccatca ccacgcacgc gcggaccgag gacgagggcg
    13261 tgcgcgtggc cggcgccggg ggcgtcatcg cgcagcgact cgcggtgccc gcggagggcg
    13321 gcacgacggt ggaggtggcc gacctcttct tcaacacgcc ggcgcggctc aagttcctca
    13381 agtcaccgac cacggagctc gccgcgtccc tgcgcctgct cacccagctc gccatcgcgc
    13441 acccgggcac gcagttccgt gcgaccagca acgggcggcc cgtgctcacg gcgccgcgcg
    13501 ccggcgacct ccgcgcgcgt gtgggcgcgc tctggggctg ggacatagcc ggacgcctgg
    13561 tggacgtcgc ccgcgccgag cacggcgtgg aggtgcgcgg ggtggcgagt ccgcccgacc
    13621 tgacgcgcgg cgggcgcgac gacgtggtgg tcgtggtcaa cggccgcccg gtgcgcgacc
    13681 ccgcgctctt cgtggccgtg gtgcacgcgt accggccgct gctggcgcgc gatcggttcc
    13741 ccctcgtcgc cctggacgtt cgcctgccgc tcgccgacgt cgacgtgaac gttcacccga
    13801 cgaaggcgtg ggtccgcttc cggcaccccc ggctcgtgca ggaaatggtc gt
//
LOCUS       DATIAZ010000185         5202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1231090, whole genome shotgun sequence.
ACCESSION   DATIAZ010000185 DATIAZ010000000
VERSION     DATIAZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231090"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..1668
                     /locus_tag="VLF19_06585"
     CDS             442..1668
                     /locus_tag="VLF19_06585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE92955.1"
                     /translation="MSDLPLAGIVVLDLTRVLAGPYCTRLLADLGARVIKIERPGDGD
                     DQRRNYLQIEPGRRDQSTYFIRVNAGKESVAVDMARPEGQAIIHDLARVADVLVENFM
                     PGVVTRLACDYETLRTVKPDLVYCSISGFGQTGPWRARPAFAHIVNAISGLMHLEQGD
                     EAAPRSSNLQAADVLAGTHAAAAILSALVRKGRTGQGAHLDVSMLEALVAADSVTYAA
                     VLNGGEEHGSPRPGMIVARVGERHVAMQFVGAEHLWPRLLALMGRPELERDPRFDSSD
                     RRRANWRELRAIVEAWLATFATSEDALRALAEARIPCAPVLRPAEVVASAHLAERQFF
                     PAVPHPGRGSVRVTASPYHLDGRPLHPRDGAPYRVGEHTRSVLGGLLGYSAERIEALR
                     TTGVVGAVAAAAPSTR"
     gene            complement(1890..2603)
                     /locus_tag="VLF19_06590"
     CDS             complement(1890..2603)
                     /locus_tag="VLF19_06590"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92956.1"
                     /translation="MRRLGVLVPAGNPTIEPELYRMAPPSVTIHFARLDSPAGEPGGA
                     DGMERRALGYLEALPAAARALAAVRPDIVVLSHTAVSYLTGFPSEPPLLERVARLAGT
                     RAITASLAILAALAHLRVRRIALATPYPESIAAAGRAYWEAAGLEVVAQRRLDGVSNI
                     YEETEERAYALGRGTDVPGADALLISGTGLPTAGIVERLETDIAKPVVTSQTATLWFA
                     LRAVGLEEPVGGYGRLLAE"
     gene            complement(2608..4005)
                     /locus_tag="VLF19_06595"
     CDS             complement(2608..4005)
                     /locus_tag="VLF19_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSE92957.1"
                     /translation="MSRIDPVWASVAELSRAFGERTLSPVDVVEALLERIRKRDPGLH
                     AFIAVYDADARLAAEAADKAIRAGHRIGPLHGVPIALKDLVDLQGRVTTGGSRVWKDR
                     VSPVTATLAERLIAAGMIVIGKTHTVEFAMGSFGTNTHLGTPRNPWDPAVHRTPGGSS
                     SGSGVAVAAGLAPVAIGTDTGGSVRLPAAWCGVVGLKVTAGRISTYGVLPLSTTLDTP
                     GPLARSVEDAALIFRALNGPDPRDPQTLAWAPADPVPALRRGVAGLQLAVMPDAERAG
                     VTDEVLAAYDASVDALVKLGARIVRPALPHRFSDYAAATGRIIGAEGYRFVGHLVDDM
                     SLPVDPHVRPRIQLGRDISARDYLMTLAERDERAREFAAALADADALLTPTTQTAALP
                     IDQVDQSGTAAYFTRAGNYLGLCGLAVPNGFTATGLPTSLQILCHPGDESTALRIGWA
                     YEQATAWKARRPPEA"
     gene            4295..4483
                     /locus_tag="VLF19_06600"
                     /pseudo
     CDS             4295..4483
                     /locus_tag="VLF19_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            4503..4934
                     /locus_tag="VLF19_06605"
     CDS             4503..4934
                     /locus_tag="VLF19_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HSE92958.1"
                     /translation="MSVTPPEPDRPAQASIDKMWNYARKYAEKSGTSFHPGEGVTETV
                     VIGLARHVDLYGRPLCPCNFYPDPAAEAKQRRWICACDEMQQYKYCHCLLFVTPEGLP
                     ITEYLPQDHEGRAVYGLVRDPTPDRGRATARVLEKQRAEPS"
BASE COUNT          716 a         1909 c         1879 g          698 t
ORIGIN      
        1 gcgcccccga gacgatccgg atgctcacct ggcagatggc ccacgacagc ggccggactc
       61 gcgagttccg cggcttcgcc gagcagtgca tggccgagta cgacctggag ggctggaccg
      121 tccccgacct catcacgccg gaggacgtca acctgttctt gaagtcccgg ccgaagtaag
      181 gcgctccgcc gccggtctcg gcggggaatg ccggcggtgg tgtccgcgcg ctcacccccg
      241 cgctccgagc gccagcaggg cggcgtcgat cacgcgctgc tgcgcccgcc cgatgatagc
      301 gcaggcgggt cgagcgcggg ggcgcgcggc gggacggagc cggaagcgca gcgacactgg
      361 aggagtcgac cctccccgtg gtggccctgc gcgcgacggg gcgtcccgcc ggccagggcc
      421 ggctggtaga atcgctgccc gatgagcgat ctacctctcg ccggcatcgt cgtcctcgac
      481 ctcacgcgcg tgctcgccgg accctactgc acgcgcctgc tcgccgacct cggggcgcgc
      541 gtgatcaaga tcgagcgtcc gggcgacggc gacgaccagc ggcgcaacta tctgcagatc
      601 gagcccggcc ggcgcgatca gagcacgtac ttcatccgcg tgaacgcggg caaggagagc
      661 gtggccgtcg acatggcgcg gcccgagggt caggcgatca tccacgacct ggcgagggtg
      721 gccgacgtgc tggtggaaaa cttcatgccc ggtgtggtga cgaggctcgc ctgcgactac
      781 gagaccttgc gcaccgtcaa gccggacctc gtctactgct cgatctcggg cttcggccag
      841 acgggcccct ggcgggcgcg tcccgccttc gcccacatcg tcaacgccat ctcgggcctc
      901 atgcatctcg agcagggcga cgaggcggcg ccgcgttcct ccaacctcca ggccgccgac
      961 gtgctggcgg gcacccacgc ggcggccgcg atcctgagcg cgctcgtccg gaagggccgc
     1021 acgggccagg gcgcgcacct cgacgtctcc atgctcgagg cactcgtggc cgccgacagc
     1081 gtgacctatg cggcggtgct caacggcggc gaggaacacg gcagcccccg gcccggcatg
     1141 atcgtggccc gcgtgggcga gcgccacgtg gccatgcagt tcgtgggcgc cgagcatctc
     1201 tggccgcgac tcctggcgct gatgggccgg cccgagctgg agcgcgatcc ccggttcgac
     1261 agcagcgaca ggcgccgggc gaactggcga gagctccgcg cgatcgtcga ggcctggctg
     1321 gccaccttcg cgacgagcga agacgcgctc cgcgccctcg ccgaggcgcg catcccctgt
     1381 gcgcccgtgc tgcgtcccgc cgaggtcgtc gcctccgcgc acctcgcgga gcgacagttc
     1441 tttcccgccg tgccccatcc cggccgtggc tccgtgcgcg tgacggccag tccgtaccac
     1501 ctggatggac ggcccctgca cccgcgagac ggggcgccct atcgcgtggg cgagcacacg
     1561 cggagcgtgc tcggcgggct tctcggctat tccgccgagc gcatcgaggc gctgcggacc
     1621 accggcgtcg tcggcgccgt ggcggccgcg gcgccgtcga ctcggtgagg ccggcggatg
     1681 gcctactggc ccaccgccgt ggccagccgc acccttcccg ccgtcagcac ggcgctcggg
     1741 ctgatcacca cgcccgtcgt cagcgtcgcc gccgcgacgc tctggctcgg ggagccgttg
     1801 aggccgggct cgtcgccgcc atcgtgctca tcctgggcgg catcgccatc ggctcgaccg
     1861 gcggccgcga ccctgcttgg tctcgccagt cactccgcca gcagccgtcc atagccgccc
     1921 acaggctcct cgagccccac ggcccggagc gcgaaccaca gcgtggccgt ctggctggtg
     1981 acgaccggtt tggcgatgtc ggtctccagg cgctcgacga tcccggcggt cggcagcccg
     2041 gttccgctga tgagcagggc gtcggcgccg gggacgtccg tcccccggcc gagcgcatac
     2101 gcgcgctcct ctgtctcctc gtagatgttg ctcaccccgt cgagccgacg ttgcgccacg
     2161 acctcgagcc ccgcggcctc ccagtacgcg cgccccgcgg cggcgatcga ttccggatac
     2221 ggcgtggcca gcgcgatccg ccgcacgcgc aggtgcgcga gcgccgcgag aatcgcgagg
     2281 gaggccgtga tcgcgcgcgt tccggccaga cgagcgacgc gctcgagcag cggtggctca
     2341 ctcgggaagc ccgtgaggta gctcaccgcc gtgtgcgaga gcacgacgat gtcgggcctc
     2401 acggcggcca gcgcgcgcgc cgccgcgggc agggcctcga ggtaaccgag ggcccttcgt
     2461 tccatgccgt cggcgccgcc cggctccccg gccgggctgt cgagccgcgc gaagtggatc
     2521 gtgaccgacg gcggcgccat ccggtacagc tccggctcga tggtgggatt gcccgccgga
     2581 accagcacgc cgagtcgccg catggcgcta cgcttccggg ggccggcgcg ccttccaggc
     2641 ggtcgcttgc tcgtacgccc agccgatgcg cagcgccgtg ctctcgtccc cgggatggca
     2701 gaggatctgg agggacgtcg gcaggcccgt cgcggtgaag ccgttcggta ccgcgaggcc
     2761 acacagcccg aggtagttcc ccgcgcgggt gaagtacgcc gcggtgccag actggtcgac
     2821 ctggtcgatc ggcagcgccg ccgtctgcgt cgtcggcgtc agcagcgcgt cggcgtccgc
     2881 cagggcggcg gcgaactcgc gggcgcgctc gtcgcgctcg gccagcgtca tgagatagtc
     2941 gcgcgcagag atatcgcggc cgagctggat ccgcgggcgg acgtgcgggt cgaccggcag
     3001 gctcatgtcg tcgaccaggt gtccgacgaa gcgatagccc tcggcgccga tgatgcgccc
     3061 ggtggccgcc gcgtagtcgc tgaaccggtg tggcagcgcc gggcgcacga tacgcgcgcc
     3121 gagcttgacg agcgcgtcga ccgacgcgtc gtaggccgcg agcacctcgt ccgtcacgcc
     3181 ggcgcgctcg gcgtcgggca tgacggcgag ctgcagcccg gcgacgcctc gccgcagcgc
     3241 gggcactgga tcggcgggcg cccacgccag cgtctgcgga tcgcgcggat cgggaccgtt
     3301 cagcgcccgg aagatcagcg cggcatcctc gaccgagcgc gccagcggcc ccggcgtgtc
     3361 gagcgtcgtg ctgagcggca gcacgccgta cgtgctgatc cgccccgccg tcaccttcag
     3421 gccgacgacg ccgcaccacg cggccggcag ccgcaccgag ccgccggtgt cggtgccgat
     3481 ggccaccggc gccagacccg ccgcgacggc cacgcccgag ccgctggagg agccgcccgg
     3541 cgtgcggtgc accgccgggt cccacgggtt gcggggtgtc ccgaggtgcg tgttggtccc
     3601 gaagctgccc atcgcgaact ccaccgtatg cgtcttgccg atcacgatca tgccggccgc
     3661 gatgagccgc tcggccagcg tggcggtcac cggcgagacg cggtccttcc acacccggga
     3721 gccgccggtg gtgacgcgtc cctgcaggtc caccagatcc ttgagcgcga tgggaacgcc
     3781 gtgcagcggg ccgatgcggt gcccggcccg gatggccttg tcggcggcct cggcggcgag
     3841 gcgcgcgtcg gcgtcgtaga cggcgatgaa ggcgtggagc cccgggtccc gcttgcggat
     3901 acgttcgagc agcgcctcca ccacatcgac cggcgagagc gtgcgctcac cgaaggcgcg
     3961 ggacagctcg gcgacggagg cccagacggg atcgatccgg ctcatgcgcg tcctcctccc
     4021 ggtgcacccg taagaggcta catcaccgcc ggccggcccg acatctcgaa gtcggccgga
     4081 gcgccgggtg gggtcgtcgc cgcgcaccgg gtgccggacc gagccgagtg ggccgagatg
     4141 ccgtatcatc tcccgtgacc gcgcgatcga ggccgccgcg cggcgccttc gaccgtcctc
     4201 catcgtggtc gagcgggacg gcgagcaaca ggaggaacgc atggcggtgc cgataaccgt
     4261 ctacaccaac atcggctgag ggccctgcca ccgagcggta gagttcctat cgcaaaaggg
     4321 cgtgccgttc accgagaaga acgtggggcg tgatcctgcc gcgcggcagg agttgatcga
     4381 gatgggcctg gtgtcgctgc cggtgctcct catcggcagc cggcgcctga cggggttcaa
     4441 cccggccctg atcgatgccg cgctcgcgga gcaacagggc tgagcgccgg gggggggagg
     4501 acatgagcgt gacgccgccc gagccggacc gcccggctca ggcttcgatc gacaagatgt
     4561 ggaactacgc ccggaagtac gccgagaagt cggggacgtc gttccatccc ggggaaggcg
     4621 tgaccgaaac cgtggtgata ggcctggccc ggcacgtgga cctctacggc cggccactct
     4681 gtccctgcaa cttctatccc gacccggccg ccgaggccaa gcagcgccgc tggatctgcg
     4741 cctgcgacga gatgcagcag tacaagtact gccactgcct gctcttcgtc acgcccgagg
     4801 gtctgccgat caccgagtac ttgccgcagg accacgaggg ccgcgcggtc tacggcctcg
     4861 ttcgcgaccc cacacccgat cggggccgcg ccaccgcccg cgtgctcgag aagcagcgcg
     4921 ccgagccgtc ctgacgggac agccctccac cccggcgacg cggtcggggc ggcggacgag
     4981 ccggcgccgg ggcgggcggg gcgtggtcgg cagccgcagg cacttgacat cgtcctcccc
     5041 gcggacgcat atttattccg tatgaagaat attaattcac ctgggacgtc ccggcggcgg
     5101 cgggacgtca tcgtccggct cgtccgcggg gggccggtgg cctcgcagga ggcgctccag
     5161 caggccctgc gccggcgcgg gatccgcgtg gcccagccca cg
//
LOCUS       DATIAZ010000186         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_968244, whole genome shotgun sequence.
ACCESSION   DATIAZ010000186 DATIAZ010000000
VERSION     DATIAZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968244"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /gene="hemC"
                     /locus_tag="VLF19_06610"
     CDS             <1..620
                     /gene="hemC"
                     /locus_tag="VLF19_06610"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSE92959.1"
                     /translation="VVTRGPGGLEALPRGAVLGTSSLRRRAFALAVRPDLVVEPLRGN
                     VDTRLRKLVDGVCDAVVLAAAGLARLGVAPPHATPLAADVFVPAVGQGILGLEVRRDD
                     TATRRLLAALDHAPTRACALAERAYLRRLGASCTTPMAAHARFEDHRLAMTGVVASED
                     GRRVLRADVAGAAGDAEALGVQLAEALLDRGAEAVTPLAPRRWRS"
     gene            617..1453
                     /locus_tag="VLF19_06615"
     CDS             617..1453
                     /locus_tag="VLF19_06615"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSE92960.1"
                     /translation="MTSPGALAGRTIVVTRSAEQAPAFVALLEAEGATVVQTPTIVIE
                     PPPSWAPLDAALDALASFAWVIFTSVNGVAMVDRRLRERRQTWTPMAGARVAAIGPAT
                     AAALEEHGLRVEIVPTDYRAEGLVERLRGRVRAGDRVLLPRAARTRDLLVAELRRLGA
                     DVHEVAAYTTRRVETAGPRLREALAAGVVDAVTFTSSSTARHFAELLTDAERKAWLPG
                     VVVASIGPITAATAAEYGLTTHVMPREYTIPALARALVEHFAREGRGRPPATARPGRR
                     SE"
     gene            1453..2439
                     /gene="hemB"
                     /locus_tag="VLF19_06620"
     CDS             1453..2439
                     /gene="hemB"
                     /locus_tag="VLF19_06620"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSE92961.1"
                     /translation="MAQAMFRPRRLREKGLLRKLVRETALAVDDLVYPLFVVHGRGVR
                     EPIGAMPGQCRLSIDELLKEAKDAASMGIPALLLFGLPEDKDARGSEAYAEDGIVQQA
                     VRAVKDTVPDLLVITDVCLCQYTSHGHCGVVERGTVKNDPTLELLARVAVSHAEAGAD
                     LVAPSDMMDGRVAAIREALDEAAFTETPIMAYSAKYASAFYGPFREAADSAPQFGDRR
                     AYQMDPANAQEALREIALDLDEGADIVMVKPALPYLDVIARAKQEFAVPLAAYSVSGE
                     YAMLKAAAQLGWLDEDRAMMEALTGIRRAGADIVITYFAKDAARRLEREREP"
     gene            2436..2663
                     /locus_tag="VLF19_06625"
     CDS             2436..2663
                     /locus_tag="VLF19_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92962.1"
                     /translation="MKRRSPVVGDVRRAVEGTGVPFDATRERRARAERDLVEPWDEIA
                     GAVSHVVDRLTLGELSARVHERLSLERATYV"
     gene            2666..>2876
                     /locus_tag="VLF19_06630"
     CDS             2666..>2876
                     /locus_tag="VLF19_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSE92963.1"
                     /translation="MDRPPVAASVLDLIGRTPMVRLNRLPSPKGARVLAKLESVNPGG
                     SVKDRIGIAMLEDAERRGALRPGGTI"
BASE COUNT          362 a         1035 c         1100 g          379 t
ORIGIN      
        1 tcgtggtgac acgtggtccc ggagggctcg aggcgctgcc ccgcggcgcg gtgctcggca
       61 cctccagcct ccgccgccgg gcgttcgcgc tcgccgtgcg tcccgatctc gtggtggagc
      121 ccctgcgcgg caacgtggac acgcggctgc gcaagctcgt cgacggcgtg tgcgacgccg
      181 tcgtgctggc ggcggccggc ctggcgcggc tcggcgtggc gccgccccac gcgacccccc
      241 tcgccgcgga cgtgttcgtg ccggccgtcg ggcagggcat cctcggcctc gaggtccgtc
      301 gtgacgacac ggcgacccgg aggctcctgg ccgcgctcga ccatgcgccc acgcgcgcct
      361 gcgcgctcgc cgagcgcgcg tacctgcgtc gcctcggggc ctcgtgcacg acgccgatgg
      421 ccgcccacgc gcgcttcgag gatcaccgcc tggcgatgac gggcgtggtg gcgagcgagg
      481 acggtcgccg ggtgctgcgt gccgacgtgg ccggcgcggc cggggacgcc gaggccctgg
      541 gtgtgcagct cgcggaggcg ttgctcgacc gcggggccga ggcggtcacc ccgctcgcgc
      601 cgaggcggtg gaggtcgtga cgagcccagg cgcgctcgcc ggtcgcacga tcgtggtgac
      661 ccggtcggcg gagcaggcgc cggccttcgt cgcgctgctc gaggccgagg gcgccaccgt
      721 cgtccagacg ccgaccatcg tcatcgagcc gccgccctcg tgggccccgc tggacgcggc
      781 gctcgacgcg ctggcgagct tcgcctgggt gatcttcacc agcgtcaacg gtgtggccat
      841 ggtcgaccgc cggctccgcg aacgccggca gacgtggacg ccgatggccg gtgcgcgcgt
      901 ggcggccatc gggccggcga cggcggccgc gctggaggag cacggtctcc gggtggaaat
      961 cgtgcccacc gactatcgcg cggaggggct cgtggagcgc ctccgcggcc gcgtccgcgc
     1021 cggcgaccgc gtgctgctgc cgcgagcggc ccggacgcgt gacctgctcg tcgcggagct
     1081 gcggcgcctc ggtgccgacg tccacgaggt ggccgcgtat actacccgac gggtggagac
     1141 cgcggggccg cggctgcgcg aggcgctcgc cgcgggcgtg gtcgacgcgg tgacgttcac
     1201 gtcgtcctcg acggcacggc acttcgccga gctgctgacg gatgccgagc ggaaggcgtg
     1261 gcttccgggc gtggtggtcg cctcgatcgg cccgatcacg gcagccacgg cggcggagta
     1321 cggactcacc acccacgtga tgccgcggga gtacacgatc cccgcgctgg cgcgggctct
     1381 cgtggagcat ttcgcccggg agggtcgcgg ccggccgccg gcaacggcga gaccgggcag
     1441 gaggagcgag tgatggcaca ggcgatgttc cgtcccagac ggctgaggga gaaggggctg
     1501 ttgcgcaagc tcgtccgcga gacggcgctg gcggtggacg acctggtcta cccgctgttc
     1561 gtcgtccacg ggcgcggcgt ccgggagccg atcggcgcga tgcccgggca gtgccggctc
     1621 tccatcgacg agctactcaa ggaggcgaag gacgcggcgt cgatgggcat cccggcgctc
     1681 ctgctcttcg ggcttcccga agacaaggac gcccgcggct cggaggcgta cgccgaggac
     1741 ggcatcgtgc agcaggccgt gcgcgcggtg aaggacaccg ttccggacct gctcgtgatc
     1801 accgacgtct gcctctgcca gtacacgagc cacgggcact gcggcgtcgt ggagcggggc
     1861 accgtgaaga acgaccccac gctcgagctg ctcgcccgcg tggcggtgtc gcacgccgag
     1921 gccggtgccg acctggtcgc gccctcggac atgatggacg gacgggtggc cgcgatccgc
     1981 gaggcgctcg acgaggcggc cttcaccgag acgccgatca tggcgtactc ggccaagtac
     2041 gcctcggcgt tctacgggcc gttccgcgag gccgcggact cggccccgca gttcggcgat
     2101 cggcgcgcct accagatgga cccggccaac gcccaggagg ccctgcgcga gatcgcgctc
     2161 gacctggacg agggcgccga catcgtcatg gtgaagccgg ccttgccgta cctcgacgtc
     2221 atcgcccgcg ccaagcagga gttcgccgtg cccctggccg cctactcggt gtccggcgag
     2281 tacgcgatgc tcaaggccgc cgcgcagctc ggctggctcg acgaggaccg cgccatgatg
     2341 gaggcgctca ccgggatccg gcgcgccggc gccgacatcg tcatcacgta cttcgccaag
     2401 gacgccgcgc gacgcctgga gcgcgagcgg gagccttgaa gaggcggtca cccgtggtgg
     2461 gcgacgtgcg gcgcgccgtc gagggcaccg gcgtgccgtt cgacgccaca cgcgagcgcc
     2521 gcgctcgggc cgagcgcgac ctcgtggagc cgtgggacga gatcgcgggg gccgtctcgc
     2581 acgtcgtcga tcgcctcacg ctcggcgaac tgtcggcacg cgtgcacgag cggctgtcgc
     2641 tcgagcgcgc gacgtacgtt tgagcatgga ccgtccgccc gtcgccgcga gcgtgctcga
     2701 tctcatcggc cggacgccga tggtgcgact caaccggctg cccagcccga agggcgcccg
     2761 cgtcctcgcc aagctcgagt cggtcaaccc gggcggcagc gtcaaggacc gcatcggcat
     2821 cgcgatgctg gaggacgccg agcggcgcgg tgccctccgg cccggaggca cgatcg
//
LOCUS       DATIAZ010000187         4243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_968804, whole genome shotgun sequence.
ACCESSION   DATIAZ010000187 DATIAZ010000000
VERSION     DATIAZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968804"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VLF19_06635"
     CDS             complement(<1..218)
                     /locus_tag="VLF19_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSE92964.1"
                     /translation="MPPARPEAARAAEAPKLGRPAEASDPTKVAATPPPARETPRPAA
                     GVTAGAPYWVQVGAFKDEATAKRLVDKLR"
     gene            complement(945..2276)
                     /gene="der"
                     /locus_tag="VLF19_06640"
     CDS             complement(945..2276)
                     /gene="der"
                     /locus_tag="VLF19_06640"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSE92965.1"
                     /translation="MIVIVGRPNVGKSSLFNRLVGRRRALVRDVPGVTRDRLYGRVAF
                     ERWQATVVDTGGLDPSSTAPLVEGVRRQVLAAVEEADLVLLVVDARDGLTALDEEIAR
                     LLRRAGRPVVLVANKVETPAQEVGLVDFYRLRLGEPMAVSAEHGRGVAEMLEALRERG
                     PAPAAVATPHDEPVHIAVIGRPNVGKSSLVNAMVGSDRVLVHAEPGTTRDAVDTALVY
                     RGRPYVLVDTAGIRRKGRVSEALEKLAVVMALKSLERCQVAVLVLDASEGLTAQDTHI
                     AGYAHEAGRASVIVVNKWDLVPRGVVRRAEVVDQLRERLPFLDYAPICFTAAPRGEGL
                     PELFDAIDTVAAAARRRVAAGEGTRVLLDALERRPVSLGGTPLTLQSAAQVGTAPPTF
                     VVRVNKPGDIHFSYERYLLKALRHAFGFPGSPIRLAFRKAVTGRARRGLRR"
     gene            complement(2398..3744)
                     /locus_tag="VLF19_06645"
     CDS             complement(2398..3744)
                     /locus_tag="VLF19_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HSE92966.1"
                     /translation="MRAEPRAAGEARGVVVASVRRRTSAANAGLRPGDRILAINGVAL
                     RDAIDFQLHAADECLRLSVEREGMPRHVALARQGPDIGLELEPPRPGEIATCANKCVF
                     CFIHQLPRGMRRSLYVKDDDFRLSFLHGNYITLTDLDEADLARIEEQRLSPLYVSVHA
                     TDPELRHRLLGRPRVPADVLPRLERLARAGIRVHAQIVLCPDLNDGPHLARTVRELAQ
                     RHPQVVTVAVVPVGLTRHRERLPALRLLTPGEARALVDTAHAWQVEFLASLGSRFVFL
                     ADEIYLLAGRALPETEAYEGFTVAEDGIGLARRFEDDLAQALPRLPRTLPGRTVTVVS
                     GEMYGPRLSALLAPLAARGVVARVAVVPNDFFGRTIGVAGLLTGRDVQRHLARIDTPG
                     EEVLVPSVAVRDVEGVFLDDLTPGDLARDLGLPVRVVEPTARALVRALTHHTKMDR"
     gene            complement(3752..>4243)
                     /locus_tag="VLF19_06650"
     CDS             complement(3752..>4243)
                     /locus_tag="VLF19_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSE92967.1"
                     /translation="PGWALAVFVGAALTDLLDGWVARSRGMSSRLGAFLDPVADKLLL
                     TASFVTLTYLKAVPFWITAVVISRDMMLVVGALTMHMVGVRIYPKPSWAGKAAMFFQV
                     LTVLTGVLARWFELPLTPWAIMWLAAVFTIVSGLQYLVQGVRFLNAANAAERDESETT
                     LLR"
BASE COUNT          621 a         1524 c         1555 g          543 t
ORIGIN      
        1 cgcagcttgt cgacgagtcg cttggcagtc gcctcgtcct tgaaggctcc gacctgcacc
       61 cagtacgggg cgccagcggt gacgccggcc gccggccgcg gcgtctcacg cgctggcggc
      121 ggagtcgcgg cgaccttcgt cggatcgctg gcctcggcgg gcctgccgag cttcggcgcc
      181 tccgcggccc tcgccgcctc gggccttgcc ggcggcatcc gtgggccatc ggccgtcgcc
      241 accgtcggcg gtggatcggg cttgggagat tctttcgagg gctcgatggc tctggcggcc
      301 ggcgctgctg gcggaggggt ggagccgggt ggcgcgacgt cctgtgtgag cggtgcgtcc
      361 tgtcgcgctg gcgcggccgg tcgtggtatc gacggcggtg gcgacgccgc cggcggcctc
      421 accgcgaccg cccgcttcgc cgtcggcgcc gtcatcacgt cgagcgcgta agggaccgcg
      481 accgccgcaa cgacgccgag aacgaggacg accacgaggg cacgaaacca gagcgcggag
      541 aagatcgacc gcggcgtctc ttcgtcgtga ttctcgaact cgcgctggcc tctttccacc
      601 atgcgcgtca tcttagcata ggggttgcgg tttccttgcg ttacgagcgc gagaacgcga
      661 gctcgatcgc cctcgcccgg cgaactcctc gagcacccag cccggcggcg gcccctccac
      721 cgacagccgc gacaacggtc gtgtccggga ggaagatccg cagaccccgg acgggtccgc
      781 gaccggctcg cgagggcagc ggggctcgac gggcgtcgcc ggcaccacga cggtccggtt
      841 cacgaccccg gtcgcccgga ggtcatcgac gtcggagggc gtcaccgagg tcaggcgcca
      901 gacggcctgc ccgaacgcgg cgacgcggcg ggctcggtcc tggttcagcg gcgcagccct
      961 cggcgggcgc gcccggtcac cgccttgcgg aacgccagcc ggatcgggga gcccgggaag
     1021 ccgaaggcgt ggcgcagcgc cttcagcaga taacgctcgt aggagaagtg gatgtcgccc
     1081 ggcttgttga cccgcacgac gaaggtcggc ggcgccgtcc ccacctgcgc cgccgactgc
     1141 agcgtgagcg gcgtgccgcc gagcgagacc ggccgccgct ccagcgcgtc gagcagcacc
     1201 cgtgtcccct cacccgctgc gacgcgccga cgggccgccg cggcgaccgt gtcgatcgcg
     1261 tcgaacagct cgggcagccc ctcacctcgc ggggcggcgg tgaagcagat cggcgcatag
     1321 tcgaggaacg gcaggcgctc gcggagctgg tcgacgacct ccgcccgtct caccactccc
     1381 cggggaacca ggtcccactt gttgacgacg atcaccgagg cgcgcccggc ctcgtgcgcg
     1441 tagcccgcga tgtgcgtgtc ctgtgcggtc aggccctcgg acgcgtcgag caccaggacg
     1501 gcgacctggc accgctccag gctcttgagg gccatcacca ccgcaagctt ctccagcgcc
     1561 tcgctcacgc gccccttgcg gcgtatgccc gccgtgtcca ccaggacgta gggccgcccc
     1621 cggtacacga gcgccgtgtc caccgcatcg cgcgtggtac cgggctcggc gtggaccagc
     1681 acgcggtcgc tgccgaccat cgcgttgacg agcgaggact tgccgacgtt ggggcgtccg
     1741 atgacggcga tgtgcaccgg ctcgtcgtgg ggcgtggcga ccgccgcggg cgcgggaccg
     1801 cgctcgcgca gcgcttccag catctcggct acgccgcgtc cgtgctccgc ggacaccgcc
     1861 atcggctcgc cgaggcggag gcggtagaaa tcgacgagcc cgacctcctg ggccggcgtc
     1921 tccaccttgt tggccacgag caccaccggt cggcccgcgc ggcgaagcag gcgcgcgatc
     1981 tcctcgtcga gcgcggtgag cccgtcgcgc gcgtcgacga cgagtaacac gaggtcggcc
     2041 tcctccaccg ccgcgaggac ctggcggcgg acaccctcga cgagcggcgc ggtggacgaa
     2101 gggtcgaggc cgccggtatc gaccaccgtc gcctgccacc gctcgaacgc gacccggccg
     2161 tagagccggt cgcgcgtgac accgggaacg tcgcgcacga gcgcgcgccg ccgtcccacc
     2221 aggcggttga agagcgacga cttgccgacg ttgggccggc ccacgatcac gatcacggga
     2281 cggagcgtgg cgaccgggcc gtgagcggta gcggctccgg gcatcacggt agccagtgac
     2341 gcgaggccag gtcgctgcac ggatgacctg accggagagg gtatggctgc ttcggcctca
     2401 ccgatccatc ttcgtatggt gcgtcagtgc tctcacgagc gcacgcgcgg tgggctcgac
     2461 gacgcggacg ggaaggccga ggtcacgggc gaggtcgccg ggggtgaggt cgtccaggaa
     2521 gacgccttcc acatcgcgga cggcgacgga tggcacgagc acctcctccc ccggtgtgtc
     2581 gatgcgcgcg aggtggcgct ggacgtcgcg gccggtgagg agcccggcga cgccgatcgt
     2641 gcgcccgaag aagtcgttgg gaacgacggc gacgcgcgcg accacgcccc gcgccgcgag
     2701 cggggccagc agcgcggaca gacgcggtcc gtacatctcc ccgctgacga cggtgaccgt
     2761 ccgccccggc agggtccgcg ggagccttgg cagcgcctgc gccagatcgt cctcgaagcg
     2821 gcgcgccaga ccgatgccgt cctccgcgac cgtaaacccc tcgtacgcct cggtctccgg
     2881 cagggcccgg ccggcgagca gatagatctc gtcggcgagg aagacgaatc ggctgccgag
     2941 ggaggcgagg aactccactt gccaggcgtg cgccgtgtcc acgagcgccc gggcctcgcc
     3001 gggcgtcagg agccgcagcg cgggcagccg ctcacggtgc cgcgtgagcc ccaccgggac
     3061 cacggcgacg gtgaccacct gagggtgccg ctgcgccagc tcgcgcacgg tccgggccag
     3121 gtgcggaccg tcgttgaggt cgggacagag cacgatctgc gcgtgcacac gaatgccggc
     3181 ccgggccagg cgctcgaggc ggggcagcac gtcggccggg acgcgcgggc gtcccagcag
     3241 ccggtggcgt agctcgggat ccgtcgcgtg caccgagacg tagagcggcg acaggcgctg
     3301 ctcctcgatg cgggccaggt cggcctcgtc gaggtcggtc agcgtgatgt aattgccgtg
     3361 caggaacgac aggcggaagt cgtcgtcctt gacgtagagg cttcggcgca tcccgcgcgg
     3421 cagctggtgg atgaagcaga agacgcactt gttggcgcag gtggcgatct cgccgggccg
     3481 cggaggctcg agctcgaggc cgatgtcggg gccctgcctc gcgagcgcga cgtggcgcgg
     3541 catcccctcg cgctcgacgg acagccgcag gcactcgtcc gccgcgtgga gctggaagtc
     3601 gatcgcgtca cggagcgcca cgccgttgat ggcgaggatg cggtcgcccg ggcgcaggcc
     3661 cgcgttcgcc gccgacgttc gccgtcgcac cgaggcgacg acgacgccgc gcgcctcgcc
     3721 ggccgcgcgc ggctcggctc gcactgccac gtcaccggag cagcgtggtc tcactctcgt
     3781 cccgctcggc cgcgttcgcg gcattcagga agcgcacgcc ctggacgagg tactgtagcc
     3841 ccgacacgat cgtgaagacg gcggcgagcc acatgatcgc ccacggggtc agcggcagct
     3901 cgaaccagcg ggcaagcaca ccggtgagca cggtgagcac ctggaagaac atcgcggcct
     3961 tcccggccca ggaaggcttc ggatagatcc ggacgccgac catgtgcatg gtgagcgcgc
     4021 cgacgacgag catcatgtcc cggctgatga cgaccgccgt gatccagaag gggaccgcct
     4081 tgagataggt cagcgtgacg aacgacgccg tgagcagcag cttgtccgcc accgggtcga
     4141 ggaacgcccc caggcggctc gacatgcccc gcgagcgggc cacccagccg tcgagcaggt
     4201 ccgtcagcgc cgcgcccacg aagaccgcga gcgcccagcc cgg
//
LOCUS       DATIAZ010000188         4275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_707295, whole genome shotgun sequence.
ACCESSION   DATIAZ010000188 DATIAZ010000000
VERSION     DATIAZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707295"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VLF19_06655"
     CDS             <1..633
                     /locus_tag="VLF19_06655"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSE92968.1"
                     /translation="AFGADGDPVVRSLQFAAGALALVVALVETSHTMAYGDELTGLPG
                     RRALDELLSELGPRYALAMVDVDHFKKFNDTYGHQAGDQLLRKIAVILQSVAGGGRTF
                     RYGGEEFAVVFLGLDVEQAGPHLEALRQAIAETTFTVRAPDRPRRKPKQTTPGGGKEV
                     GVTVSIGVADSNRPGGTPAEVVKAADEALYRAKRAGRNRCAVARLSSVSG"
     gene            complement(646..1383)
                     /locus_tag="VLF19_06660"
     CDS             complement(646..1383)
                     /locus_tag="VLF19_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE92969.1"
                     /translation="MARGSIENGHWSEPAVQVAHLPSEDITRRLVIVEDESLLAEDLA
                     MRLTRLGYEVVGIAPSGEAGVEVGERMRPDLVLMDIHLRGQVDGITAAERLRDRFDIP
                     VVFLTGNADDRTFERAKAVCPLGFVIKPFDGRQLQIAVEVALARHAAERALLQAGQLR
                     AVVLLAGGAAHEINNPLTVVKGMLQLVSKTLPEGTQERKRLDMALDAAERIKDAVARL
                     AHVTRLELAESQPRVPPMLDLRRSSGA"
     gene            complement(1505..3010)
                     /locus_tag="VLF19_06665"
     CDS             complement(1505..3010)
                     /locus_tag="VLF19_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HSE92970.1"
                     /translation="MIEILAHLGAGFSDAVQPLNFGMVVLGLAIGIVAGALPGVTMLN
                     SIVLVLPFTYLMGIVPALLLMIGIYCGGVFGGSITAILFNIPGDPMNVPTAWEGYRLS
                     RRGQTRYALGLAIMASAFGGLVSALCLAFFAPPFAKIALTFSTVEFFSIVVFGLASVS
                     VLGHASATGALISLFAGMFLGTVGTEAQYGVERFTFGVPFLRTGVDFVTVLIGLFAIG
                     EILDQIATPAPSGPATAPPAGSVPRLPLLSLWPLRGPLVRGTAVGVAVGGLAGAGATV
                     SSFVAYGLEKQISREPERFGTGHSGGLVAAESANNGSTGGAMIHLLTLGIPGSAATAV
                     MMGAFLIHGVQPGPLMFSRQPEAVYTIIAGMILANVLMIALGFLAAMGFAALMRVPAP
                     VLGTFIVLFCFVGAFALRNDMADVWITMLFGILGFVMRRWDLPVPPMVMGIILGPMAE
                     QYFLTSMVSHGNDPTVFVTRPVSAVALGLSAAMVVWAMLRAARLRRRAAVA"
     gene            complement(3012..3524)
                     /locus_tag="VLF19_06670"
     CDS             complement(3012..3524)
                     /locus_tag="VLF19_06670"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HSE92971.1"
                     /translation="MAALARAAAPLAGLSAALALLAVTRGLDDVAREGQLGPGFWPRL
                     VLAGLAVTCAARLVLAWWAGATRDAGSDAGAMALPSRLRLAAAVALILLYVVAAPVIG
                     FPLATAAFVAGFMTVSGARSPGGAAVAAVGGTVGLLYLFVKVVYLPLPKGAGPFEAVT
                     LALYRALGIF"
     gene            complement(3526..>4275)
                     /locus_tag="VLF19_06675"
     CDS             complement(3526..>4275)
                     /locus_tag="VLF19_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HSE92972.1"
                     /translation="AAGNTGLAQVLNGKPDGYTIAVYIQDTLMTIPMGLARHKVEDLE
                     WIVRTQVADSFLFVKSDGPFKAIQDLLKHAQANPGKVRVASTGFGSVDDVSVRYLEKK
                     GHKMTTVPYPKPAERYAAALGGHAEVLYEQAGDVLQYLKAGQLKPLVIFAEKRHPAFA
                     DVPTSKELGFDITLPQFRGVVARKGTPPDRVQVLAAAFKQAMDTPQWKKFAADWYMSP
                     ESYQGPEQFGKWVASEVATLEQLVKEFGLRK"
BASE COUNT          701 a         1493 c         1532 g          549 t
ORIGIN      
        1 gccttcggcg ccgacggcga cccggtcgtg cgcagcctgc aattcgccgc cggcgcgctg
       61 gcgctcgtgg tcgccctcgt cgagacgtcg cacacgatgg cgtacggcga cgagctgacc
      121 gggctgccgg gccggcgcgc gctcgacgaa ttgctgagcg agctcggccc acgctacgcg
      181 ctggccatgg tggacgtgga ccacttcaag aagttcaacg acacgtacgg ccaccaggcg
      241 ggcgaccagc tcctgcgcaa gatcgcggtg atcctccagt ccgtcgccgg gggcggccgc
      301 accttccgct acggcggcga ggagttcgcg gtggtcttcc tggggctcga cgtcgagcag
      361 gccggtccac acctcgaggc gctcaggcag gccatcgccg agacgacgtt caccgtgcgc
      421 gcgcccgacc gtccgcggcg gaagccgaag cagacgacac cgggcggcgg caaggaggtg
      481 ggcgtcaccg tgagcatcgg ggtcgccgac tcgaaccgac cgggcgggac gccggcagag
      541 gtcgtcaagg cggccgacga ggcgctctat cgcgccaagc gcgccggccg gaaccgctgt
      601 gcggtggcgc gcctcagcag cgtgtcgggt tgaacagccg cggggtcagg ccccgctcga
      661 cctccgcaga tcgagcatcg gcggcacccg tggctgcgac tccgccagct cgaggcgggt
      721 gacgtgggcg agccgcgcga ccgcatcctt gatgcgctcg gcggcgtcga gggccatatc
      781 cagccgcttg cgctcctgcg tgccctcggg gagggtcttg ctcaccagct gcagcatgcc
      841 cttcacgacg gtgagcgggt tgttgatctc gtgcgccgcc ccgcccgcga gtagcaccac
      901 cgcccggagc tgcccagctt ggagtagcgc ccgctcggcg gcgtgccggg ccagcgcgac
      961 ctcgacggcg atctgcagct gccgcccgtc gaatggcttg atcacgaagc ccagcggaca
     1021 gacggccttt gcccgctcga aggtccggtc gtcggcgttt cccgtgagga acaccaccgg
     1081 gatgtcgaac cggtcgcgga gccgctcggc cgccgtgata ccgtcgacct gaccgcgcag
     1141 gtggatgtcc atcaggacga gatccgggcg catgcgctcc ccgacctcga cgccggcttc
     1201 tcccgagggc gcgatgccga cgacctcgta gccgagcctg gtcagccgca tggccaggtc
     1261 ctcggccagg aggctctcgt cctccacgat gacgagacgc cgcgtgatgt cttccgacgg
     1321 taggtgcgcg acctggacgg ctggctcgct ccagtgtccg ttctcgatcg acccgcgggc
     1381 catgacggac gccgagggag caagcgtcgt tccattacat ggatcccaag gatttcgaac
     1441 acataggcgt cgaccgcgga cgcgaacgat cgtcgcgtga cacgtcttcc gtgccaggac
     1501 gcgctcaggc aacagccgcg cgcctccgga ggcgcgcggc ccgcagcatc gcccagacca
     1561 ccatcgccgc cgacagcccg agcgccaccg cgctcaccgg gcgcgtgacg aagaccgtgg
     1621 ggtcgttgcc gtgcgagacc atcgaggtga ggaagtactg ctcggccatc ggcccaagga
     1681 tgatgcccat caccatcggg ggcaccggga ggtcccaccg gcgcatgaca aagcccagga
     1741 tgccgaacag catggtgatc cagacgtccg ccatgtcgtt gcgaagcgcg aaggcgccga
     1801 cgaagcagaa cagcacgatg aaggtgccga ggacgggcgc cggcacgcgc atgagcgcgg
     1861 cgaagcccat ggcggccagg aaccccagcg cgatcatcag gacgttggcg agaatcatcc
     1921 cggcgatgat cgtgtagacc gcctccggct gccgggaaaa catcagcggc cccggctgga
     1981 cgccgtggat caggaacgcg cccatcatga ccgccgtcgc cgcgctgccg gggatgccca
     2041 gcgtcaggag gtggatcatg gctccgcccg tggagccgtt gttcgcgctc tcggcggcca
     2101 ccaggccgcc ggagtggccg gtgccgaacc gctccggctc gcgcgagatc tgcttctcga
     2161 gcccgtacgc gacgaacgac gacaccgtcg cgcccgcgcc agccagcccg ccgacggcga
     2221 cgccgacggc ggtgccgcgg acgagcgggc cgcggagcgg ccagagggaa agcagcggca
     2281 ggcgcggcac cgagcccgcg ggcggagcgg tcgccgggcc ggacggcgcg ggcgtcgcga
     2341 tctggtcgag gatctcgccg atcgcgaaga gcccgatcag caccgtgacg aagtccaccc
     2401 ccgtccggag gaagggcacg ccgaaggtga atcgctcgac cccgtactgg gcctccgtgc
     2461 ccacggtgcc gaggaacatc cccgcgaaga gcgagatgag cgcgcccgtg gccgaggcgt
     2521 ggccgagcac cgagacgctc gcgagaccga agacgacgat cgagaagaac tcgacggtgg
     2581 aaaacgtcag cgcgatcttc gcgaacggcg gggcgaagaa ggccaggcag agcgcgctga
     2641 ccaggccgcc gaaggcggag gccatgatgg ccagaccgag cgcatagcgc gtctggcccc
     2701 ggcggctgag gcgatagcct tcccatgccg tgggcacgtt catcggatcg ccggggatgt
     2761 tgaagaggat cgccgtgatc gagcctccga acacgccgcc gcagtagatg ccgatcatca
     2821 gcagcagcgc cgggacgatc cccatgaggt aggtgaaggg cagcaccagc acgatggagt
     2881 tcagcatcgt gaccccgggc agcgccccgg ccacgatgcc gatggcgagg ccgagcacca
     2941 ccatcccgaa gttcaggggc tggacggcgt cggagaaccc ggcgcccagg tgggcgagga
     3001 tctcgatcac gtcagaagat cccgagcgcg cggtagaggg ccagtgtgac ggcctcgaac
     3061 gggccggcgc ccttgggcag cggcaggtac acgaccttga cgaacaggta gaggaggccg
     3121 acggtgcctc ccaccgccgc gaccgcggcg ccgcccggcg accgcgcgcc ggagacggtc
     3181 atgaagccgg cgacgaacgc cgccgtggcc aggggaaacc cgatcaccgg cgcggcgacg
     3241 acgtagagga ggatgagggc gaccgcggcg gccagccgca gccgcgacgg cagcgccatc
     3301 gccccggcgt cggatcccgc gtctcgcgtc gcgccggccc accaggcgag aacgagccgc
     3361 gccgcgcacg tgacggcgag cccggccagc accagccgtg gccagaaccc ggggccgagc
     3421 tgtccctcgc gcgcgacgtc gtcgagcccg cgcgtgacgg ccagcaacgc cagcgcggcg
     3481 gagagcccgg cgagcggcgc agcggcacgc gcgagcgccg ccatgctact tgcggagccc
     3541 gaactccttg acgagctgct ccagcgtggc cacctcgctc gccacccact tgccgaactg
     3601 ctcgggcccc tgatagctct ccgggctcat gtaccagtcc gcggcgaact tcttccactg
     3661 cggcgtgtcc atcgcctgct tgaaggcggc cgcgagcacc tgtacgcggt cgggcggcgt
     3721 ccccttgcgg gcgacgacgc cgcggaactg cggcagcgtg atgtcgaagc ccagctcctt
     3781 cgacgtcggc acgtcggcga aggccgggtg gcgtttctcg gcgaagatga cgagcggctt
     3841 gagctggccg gccttgaggt actggagcac gtcgcccgcc tgctcgtaga gcacctcggc
     3901 gtggcctccg agcgccgcgg cgtagcgctc ggcgggcttc ggatacggca ccgtcgtcat
     3961 cttgtggccc ttcttctcga ggtagcgcac ggagacgtcg tccacgctgc cgaagcccgt
     4021 ggaggccacg cggaccttgc cgggattcgc ctgggcgtgc ttgagcaggt cctggatggc
     4081 cttgaacggg ccgtcggact tcacgaacag gaacgagtcg gccacctgcg tgcgcacgat
     4141 ccactcgagg tcctcgacct tgtggcgggc gaggcccatc gggatggtca tgagcgtgtc
     4201 ctggatgtac acggcgatcg tgtagccgtc gggcttgccg ttgagcacct gggcgagccc
     4261 ggtgttgccg gccgc
//
LOCUS       DATIAZ010000189         4445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_840218, whole genome shotgun sequence.
ACCESSION   DATIAZ010000189 DATIAZ010000000
VERSION     DATIAZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840218"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VLF19_06680"
     CDS             complement(<1..268)
                     /locus_tag="VLF19_06680"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HSE92973.1"
                     /translation="MASSPSATARAEPAVVARGARVRTTPDGAAAAEGGADVYRVGID
                     VGGTFTDLVAVDETGRVTIAKTASTPADPSDGLLEGLRLLARELR"
     gene            complement(159..1895)
                     /locus_tag="VLF19_06685"
     CDS             complement(159..1895)
                     /locus_tag="VLF19_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSE92974.1"
                     /translation="MATMLDPITVSVLTHRFEAIVQEMGEAMLRTAYSQILNSSRDFS
                     TAICDAGARLVAQAEHVPIHVGAIPWAVESVRDFFTGRVRPDDVFLLNDPYHGNNHLP
                     DLTAFVPVFAGDRLLFWSINRAHQSDIGGSTHGAYNPGATEIWQEGLRITPLKLYDGG
                     ALRDDVLEMVATNVRHPRDFLGDLRAMIGSARVGERRLLTLVDEYGADAVTTAVDQIL
                     DGAEVQARACVRSWKDGVYRGETILDDDGHRFTDIPVRATVTKKGDGLTVDLTESHQQ
                     VDGFINSSFPNTMSAVHMAFAYLIDPRTPKNSGTFRPVKVIARQGTIVWPNPPAPVTL
                     ATNHCAQDIAEAIIKALAPACPDRAIAGWGRRFRIAIKGVNPRTRRPFIWHMFHARPG
                     GGATVVGDGWETAGEGQAAGGIKFGSVEVAEARFPLTFEHHEFRAGSGGDGMHRGGVG
                     AVLRLRVDVAESAVANTAGDGVRHAPYGILGGEDGLPHRYRLLSRGRERMLRTKEVGV
                     PVLPGDVFLVESSGGGGYGPPSRRSRAARERDVTNGFVAERDSPRGAGGRGQGRARPN
                     DSGRRGGRRGGR"
     gene            2051..2257
                     /locus_tag="VLF19_06690"
     CDS             2051..2257
                     /locus_tag="VLF19_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92975.1"
                     /translation="MTEILDVRWCDAHAPARDGSDDATVNCEGFMSGSAEAGGDANRR
                     WCQLLHSGSEARRPRLRRKAARPA"
     gene            complement(2344..2688)
                     /locus_tag="VLF19_06695"
     CDS             complement(2344..2688)
                     /locus_tag="VLF19_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSE92976.1"
                     /translation="MTIKRLNPGPRLSGAVVHGDTVYIAGQVSGDTPTVKGQTEAILK
                     KFDELLAAAGSHKSKLLSANVYLASMSGYDEMNAAWEAWVDPRNTPARATVEAKLAAP
                     KYLVEIMGVAAI"
     gene            complement(2685..3011)
                     /locus_tag="VLF19_06700"
     CDS             complement(2685..3011)
                     /locus_tag="VLF19_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE92977.1"
                     /translation="MPDDLLVSGAPDDVLFRSVTALRRLGARIIRYDAEALTLEARIV
                     QRLGAVLVRLRAAEAEPGRTRLAVETDTALRVPWLGHRRSLVRGFARELAQITTTPMT
                     GRAEDR"
     gene            3151..3933
                     /locus_tag="VLF19_06705"
     CDS             3151..3933
                     /locus_tag="VLF19_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92978.1"
                     /translation="MGLLTGKTAIITGASKGIGREMSQMFAREGARLVCAARSAKLVE
                     ETAELVRKAGGQAVAVVGDVATEDGARHTVAQGMHAFGRIDTLVNNAGDGGPTKPVQE
                     YTTEDWFYTVNSCLTSSYMMTRFTVPEMIKAGGGAIVNISSMAGRRGLAFRIGYCASK
                     AGQVGMTYGMALELAPHNITVNAIAPGAVEGDRIDSVIAGQARVRGISAEDMRRAMLE
                     RSPLKRMTRAQDIAALAVFLCSEGARNLSGQCIAVNAGEPAG"
     gene            4110..>4445
                     /locus_tag="VLF19_06710"
     CDS             4110..>4445
                     /locus_tag="VLF19_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSE92979.1"
                     /translation="MGLRVGDFAPSASTLRVRGKGNKDRVVVLTRLGAQAVAKYLAME
                     ATGPSDAALFVTAAGRPIIYRAVTRAVRRARRLGIHVHPHMFRHSYGTELLERGADIR
                     DIRDLLGHES"
BASE COUNT          644 a         1545 c         1586 g          670 t
ORIGIN      
        1 tgcggagctc acgcgcgagc agccgcagac cctcgagcag cccgtccgac gggtccgcgg
       61 gcgtcgaggc ggtcttggcg atcgtcacgc ggccggtctc gtccacggcc acgaggtcgg
      121 tgaacgtgcc gccgacgtcg atgccgacgc ggtacacgtc agcgcccccc tcggcggccg
      181 ccgcgccgtc cggagtcgtt cggacgcgcg cgcccctggc cacgaccgcc ggctccgcgc
      241 gggctgtcgc gctcggcgac gaagccattg gtgacgtcac gctcgcgggc ggccctggac
      301 cggcgcgacg gcgggccgta accgccgccg cccgacgact cgacgaggaa cacgtcgccg
      361 ggcagcacgg gcacgccgac ctccttcgtc cggagcatgc gctcccggcc gcgcgagagc
      421 aggcggtagc ggtggggcag gccgtcctcg ccgccgagga ttccgtaggg cgcgtgtcgt
      481 acgccgtcgc cggcggtgtt cgccaccgcg gactcggcca catccacgcg cagccggagc
      541 acggcgccca cgccgccgcg atgcatgccg tcgccgcccg agcccgcccg gaactcgtga
      601 tgctcgaacg tcagcggaaa gcgtgcctcg gcgacctcca cgctgccgaa cttgatgccg
      661 cccgcggcct ggccctcgcc ggccgtctcc catccgtcgc ccacgacggt ggcgcccccg
      721 ccgggccggg cgtggaacat gtgccagatg aagggccggc gcgtgcgggg gttgacgccc
      781 ttgatggcga tgcggaagcg gcggccccag ccggcgatgg cgcggtcggg acaggccggc
      841 gccagcgcct tgatgatggc ctccgcgatg tcctgggcgc agtggttggt ggccagcgtc
      901 acgggggccg gcgggttcgg ccacacgatg gttccctgcc tggcgatcac cttcacgggg
      961 cgaaacgtcc ccgagttctt cggcgtgcgc gggtcgatga ggtaggcgaa ggccatgtgg
     1021 acggccgaca tcgtgttcgg gaacgacgag ttgatgaacc cgtccacctg ctggtgcgac
     1081 tccgtgaggt ccaccgtgag gccgtcgccc ttcttcgtga cggtggcgcg gacggggatg
     1141 tcggtgaagc ggtggccgtc gtcgtcgagg atcgtctcgc cgcgatagac gccgtccttc
     1201 cacgacctca cgcacgcgcg ggcctggacc tcggcgccgt cgaggatctg gtccaccgcg
     1261 gtggtgaccg cgtcggcgcc gtactcatcc accagcgtca gcagccgccg ctcccccacc
     1321 cgcgccgagc cgatcatggc gcgcaggtcg cccaggaagt cgcgcgggtg gcggacgttc
     1381 gtggccacca tctccagcac gtcgtcgcgc agcgcgccgc cgtcgtaaag cttgagcggg
     1441 gtgatgcgca gcccctcctg ccagatctcc gtcgccccgg ggttgtaggc gccgtgcgtg
     1501 gagccaccga tgtcgctctg gtgggcccgg ttgatcgacc agaacagcag ccggtcgccg
     1561 gcgaagacgg ggacgaaggc cgtcaggtcg ggcaggtggt tgttgccgtg gtaggggtcg
     1621 ttcaacagga acacgtcgtc cggccgcacc cggcccgtga agaagtcgcg caccgactcc
     1681 acggcccagg gaatcgcgcc cacatggatg ggcacgtgct cggcctgggc gacgaggcgg
     1741 gcgccggcgt cgcagatggc cgtggaaaag tcacggctgg agttgaggat ctgcgagtag
     1801 gccgttcgga gcatggcttc ccccatctcc tgaacaatgg cctcgaatcg atgggttaga
     1861 accgagaccg tgatggggtc gagcatggtc gccattatca cccttttctc tttgtgggcc
     1921 gggacccgac tgctagattg gaaatgatgg cgacatccag actcgccatg gtgcaatgac
     1981 cgagttcatc gccgacgccc ccgtcgtcgc acgatcgtat tgtcccggct gtgagcccga
     2041 ggccgacccc gtgaccgaga ttctcgacgt gcgctggtgc gacgcgcacg cgcccgcgcg
     2101 cgacggcagc gacgacgcca ccgtgaactg cgagggcttc atgtccggca gcgccgaggc
     2161 gggcggcgac gccaaccggc gttggtgcca actgctccac agcgggtcgg aggcgcgccg
     2221 cccccggctg cgccgcaagg cggcccgccc cgcctgattg catggggccc tgagaagccc
     2281 caagcgttcg gcacgtctcc cgctgtaccg tgccgcgcct ccttaactcg ataggctccg
     2341 tgatcagatc gcggcgacgc ccatgatctc cacgaggtat ttcggcgccg ccagcttcgc
     2401 ctcgaccgtc gcccgggccg gcgtgttcct gggatcgacc caggcctccc acgcggcgtt
     2461 catctcgtca tagccggaca tgctggcgag gtagacgttc gccgacagca gcttcgattt
     2521 gtggctgcca gcggcggcga ggagctcgtc gaacttcttg aggatggctt ccgtctggcc
     2581 cttcacggtg ggcgtatctc cggacacctg tcccgcgatg tacaccgtgt cgccgtgaac
     2641 gacggcgccg ctcaggcgcg gaccggggtt gagtcgcttg atcgtcatcg gtcttccgcc
     2701 ctccctgtca tcggtgtggt ggtgatttgc gccagctcgc gcgcgaaacc tcggacgagg
     2761 cttcgacggt gcccgagcca cggcacgcgc aaggccgtgt ccgtctcgac cgccagccgc
     2821 gtgcgaccgg gctcggcctc ggccgcccgc aagcgcacca ggacggcgcc cagccgctgc
     2881 acgatccgcg cttcgagcgt cagcgcctcg gcatcgtaac gaataatacg cgcgccgagg
     2941 cggcgcagcg cggtgacgct gcggaagagc acgtcgtctg gcgctccgct cacgagcagg
     3001 tcgtccggca cggcgcccat catcgcactt acgcggcgcg tccgcctgcc gtctgtgcgc
     3061 ctccgccgcc ttgaccggcc cgccgcgggc gggctacgct cgccgcactc gaggcggcct
     3121 ggagggccgc cgatcggagg agagcgcgac atgggactgc tgaccggcaa gaccgcgatc
     3181 atcaccggag cgagcaaggg catcgggcgc gagatgagcc agatgttcgc gcgcgagggg
     3241 gcgcgcctcg tctgcgccgc gcggtcggcc aagctcgtgg aggagacggc cgagctcgtg
     3301 cgcaaggcgg gcgggcaggc cgttgccgtc gtcggtgatg tggcaacgga ggacggggcg
     3361 cggcacacgg tcgcgcaggg catgcacgcc ttcggccgga tcgacacgct cgtcaacaat
     3421 gccggcgacg gcggaccgac caagcccgtg caggaataca ccaccgagga ctggttctat
     3481 acggtcaatt cgtgcctgac atcctcgtac atgatgacgc gcttcacggt gccggagatg
     3541 atcaaggcgg gcggcggcgc catcgtgaac atctcgtcga tggccgggcg ccgagggctg
     3601 gccttccgca tcggctactg cgcctccaag gccggccagg tgggcatgac gtacggcatg
     3661 gcgctcgagt tggcgcccca caacatcacc gtcaacgcca tcgcgcccgg tgccgtcgag
     3721 ggcgaccgca tcgacagcgt catcgccggc caggcgaggg tgcgcggcat ctccgccgag
     3781 gacatgcggc gcgcgatgct cgagcgctcg ccgctcaagc ggatgacacg cgcccaggac
     3841 atcgccgcgc tggccgtgtt cctctgcagc gagggggccc gcaatctctc ggggcagtgc
     3901 atcgcggtga acgccgggga gccggccggg tagatcgccg ggcgtgaacg cccggctcat
     3961 ccacctccag aaactggacg cgagcccgcc ggaagccggg ttctgtcccc tcgtcgcgtt
     4021 ttctcaatcg gcgcggggag ttccggcgag tgtgagccag cgccgtcaac ggggtctgta
     4081 acttcaggtg gttcggccgc ggggaggttg tggggctcag ggtcggagac ttcgccccga
     4141 gtgcctcgac acttcgcgtc cgcggcaagg gcaacaagga tcgggtcgtc gtgctgacac
     4201 ggctcggcgc tcaggcggtg gccaagtatc tggctatgga agccacgggg ccgtcagacg
     4261 cggcgctgtt cgtcacggcg gctgggcggc cgatcatcta tcgagcggtc acccgggcgg
     4321 tgcgccgggc ccgtcggctc gggatccacg tgcatccgca catgttccgg cacagctacg
     4381 ggaccgagct cctcgagcgc ggcgccgaca tccgggacat tcgcgatctg ctggggcatg
     4441 agtcg
//
LOCUS       DATIAZ010000190         8575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1103209, whole genome shotgun sequence.
ACCESSION   DATIAZ010000190 DATIAZ010000000
VERSION     DATIAZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103209"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VLF19_06715"
     CDS             <1..217
                     /locus_tag="VLF19_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HSE92980.1"
                     /translation="GFIVSGTGDIIEPDDGLIGIGSGGPYALAAARALVAHSDLDAKA
                     VATEAMRIAAAICIYTNEQITVETLSP"
     gene            214..1572
                     /gene="hslU"
                     /locus_tag="VLF19_06720"
     CDS             214..1572
                     /gene="hslU"
                     /locus_tag="VLF19_06720"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HSE92981.1"
                     /translation="MSVSPNLTPAEIVSELDRYIVGQERAKRAVAIALRNRWRRQNLP
                     SELRDEVAPKNIIMIGPTGVGKTEIARRLARLAQAPFVKVEASKYTEVGYVGRDVESM
                     IRELTELAVTMVKAEMTAAVQDKAEQAAEERLLDLLLPRRGEPFSSESLEEVGAGARE
                     ATRDKLRAQLRAGRLDDRMVELETQQHAMPMIEVFSGQGMEEMGINLKEMLSNVLPTR
                     TRRRRLRIGEARRLLVQEEAQKLVDHDEAVGLAIRRVENSGIVFIDELDKVAGREGGG
                     HGPEVSRQGVQRDLLPIVEGSAVTTKHGIVRTDHILFIAAGAFHVAKPSDLIPELQGR
                     FPIRVELDPLTREDFVRILTEPRNALIRQYVELLLTEHVTLRFLPDAVEAIAEIAMQV
                     NASTENIGARRLYTILEKLLEDVSFGAPEMPGKELTIDAGYVHQRLADITRDQDLSRY
                     IL"
     gene            1629..2462
                     /gene="argB"
                     /locus_tag="VLF19_06725"
     CDS             1629..2462
                     /gene="argB"
                     /locus_tag="VLF19_06725"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSE92982.1"
                     /translation="MEALPYIREFRGKTVVIKYGGAAMEQAELKEPFALDVILLRLVG
                     INAVIVHGGGPQIGAMMKRLGKEPRFVGGMRVTDAETVEIVEMVLVGKINKEIVGLIN
                     HHGGRAVGLSGKDADLIRAHRRGHRLPGGEEIDIGLVGEVDAVNPEPIRLLEESGFIP
                     VIAPVGVGAGGETYNINADLVAGEVAAALGAAKLIHLTDVQGIMDGQGRLVSTLARRD
                     AERLVREGVIDGGMLPKVESSLRALKGGTAKAHIIDGRQPHAILLEMFTREGIGTEIV
                     L"
     gene            2471..3664
                     /locus_tag="VLF19_06730"
     CDS             2471..3664
                     /locus_tag="VLF19_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSE92983.1"
                     /translation="MDTKTLFEWSATYHMPNFARTPIVLVRGDGLRVWDTEGREYLDF
                     GAGLAVTALGHSHPRVTGAIREAAATLLHVSNLYHNVPQIHLAKLLCEHSFADQAFFS
                     NSGAEANEAAIKMARKYGKERFATDRYEIIATHDSFHGRTLATVTATGQPKYHHGFEP
                     LVAGFKHVPYNDLKSMERAIDNRTAAILVEPIQGEGGVHVPDDDYLPGLRKLCDESGA
                     LLILDEIQTGVGRTGRLWAYEHAGVEPDVMTLAKALANGLPIGATLARREVASVMTPG
                     THGSTFGGNPFVTSVALACMTTMLDDKIPERAARVGRHLIDGLRRIAAARPAVRTVRG
                     KGLLIGAELTRPAAPVVDACREAGLLILSAGEKVVRLAPPLIVDERLCDQALGILDSA
                     LAKTA"
     gene            3661..4584
                     /gene="argF"
                     /locus_tag="VLF19_06735"
     CDS             3661..4584
                     /gene="argF"
                     /locus_tag="VLF19_06735"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSE92984.1"
                     /translation="MKHLISAGDLTRERALHLFRTAAALKQGRKAGTRGAPLSGRTLA
                     LIFEKPSLRTRVTFEVGIVQLGGRAVYLSGAEIGLGTRESVPDVARNLSLWVDGVAAR
                     VYAHATVEALARSATVPVINGLSDFEHPCQALADYFTLWERGVDLGAMRLAWIGDGNN
                     VSHSVALLGALFGTEVVVACPPGYEPNPGVQATVRRLGGRLSVVHDPRDAAAGADAIY
                     TDVWTSMGQEAERERRLEAFGRFQVNERVVGFAKPSVLVMHCLPAHRGEEITDAVLDG
                     ARSIALAQSENRLHAQKAVILDLLGGSLDDA"
     gene            4577..5854
                     /locus_tag="VLF19_06740"
     CDS             4577..5854
                     /locus_tag="VLF19_06740"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSE92985.1"
                     /translation="MPDSSSHFRARLAAPGGGAAESASPRAPKRVVLAYSGGLDTSVI
                     LRWLIERYGCEVVAFCADLGQGEELIPVRDKALRTGAQRVHIVDLREQFARDFVFPML
                     RAAAVYEGSYLLGTSIARPLIARAQVEIALEEGADAVAHGATGKGNDQVRFELTYAAL
                     APHLKVIAPWREWDLDSRSALIDFARRHDIPVPVTTERPYSTDRNLFHISYEGGVLED
                     PWAAPPVKMFLLTNAPEQAPDVPVEVLIDFEAGNPVAVDGQRLGPVALLERLNRVGGE
                     HGIGRVDLVENRFVGMKSRGVYETPGGTILHAARRALESLTLDREVLHLRDGLVPRYA
                     EMIYYGFWFAPERRVLQTLMDECVRDVTGTVRLTLYKGNVTVTGRRSPRSLYRTDFAT
                     FEADSVYRPGDAEGFINLNALRLRIRALRDRGR"
     gene            5859..6590
                     /locus_tag="VLF19_06745"
     CDS             5859..6590
                     /locus_tag="VLF19_06745"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HSE92986.1"
                     /translation="MRVDVAPTAEALAGRSVEGVVVLVIDVLRASTTIVTALANGCAA
                     VVPVADPEEARRRARALPPGTVLTAGERRGEPIQGFDLGNSPTDCTAERVRGRVIVFT
                     TSNGTRALLAARAAAAIGVAALVNVSAAATWALGAGRDVTLLCAGERGAISLEDHVCA
                     GLLAERLQREAPSATLTPAAEAAVRASCGYGKAVARLAQDSRWARHLARTGRGDDVAA
                     CLVLDAVALVPVYLPDVDKVVSGRR"
     gene            6627..7196
                     /gene="pgsA"
                     /locus_tag="VLF19_06750"
     CDS             6627..7196
                     /gene="pgsA"
                     /locus_tag="VLF19_06750"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HSE92987.1"
                     /translation="MNLPIGLTLTRIVAVPLLIVFLISSSRVHAVIAAGIFILASLTD
                     WADGVLARRRNQVTTLGTLLDPIADKLLVAAALVSLLQIDKVAAWMVVVIIGREFAVT
                     GLRAVAAHVGVIVPASRLAKWKTAGQYVAITMLIIEKGVGSPAFHVAATVVLWIALAL
                     TVVSAVDYFYRFFRKADYRAIVPGEERWS"
     gene            7193..7789
                     /gene="plsY"
                     /locus_tag="VLF19_06755"
     CDS             7193..7789
                     /gene="plsY"
                     /locus_tag="VLF19_06755"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HSE92988.1"
                     /translation="MSTLGALAAAYLIGALPFGFLVARAWGIGDIRQHGSGNIGMTNV
                     LRTAGRTPAILTLVGDVAKGYLAVAAGAAVAGGEPAATAASAVAAIVGNCWSIFLAFR
                     GGKGVATGLGAFLNLAPLAVAPAALVWLLVTLSFRYVSLGSLMAAICVPLGAALLGYG
                     AAKAAACAAGAAIVVLRHHENIGRLFAGTERRLGGRRA"
     gene            7891..>8575
                     /locus_tag="VLF19_06760"
     CDS             7891..>8575
                     /locus_tag="VLF19_06760"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSE92989.1"
                     /translation="MARLGMATCSWALAPVAAEVALRIGVVGGGSWGTGLAIHLARAG
                     AAVRLWAREAEVVEGIRRARRNPFYLKDIDCPTGIDPTGDPAEVVRDADVMLLVVPSE
                     FFALTLKGLGRIPRGVPIVSATKGLDPERHVRMSEIVAEQHPDAPVAALSGPSFAREV
                     ALGQPTACVVAAADDALATRLQAALGTREFRLYTNRDVVGVEMGGALKNVIAIATGIA
                     DGLGLGENAR"
BASE COUNT         1219 a         3062 c         3087 g         1207 t
ORIGIN      
        1 gggcttcatc gtctccggca ccggcgacat catcgagccc gacgacggcc tcatcggcat
       61 cggctcgggc ggcccctacg cgctggccgc cgcgcgcgcg ctcgtggcgc actcggacct
      121 cgacgccaag gcggtggcca ccgaggccat gaggattgcc gccgccatct gcatctacac
      181 caacgagcag atcaccgtgg agacgctgtc gccgtgagcg tcagcccgaa cctcactccg
      241 gccgagatcg tcagcgagct ggaccgctac atcgtgggcc aggagcgggc caagcgcgcg
      301 gtggccatcg cgctgcgcaa ccggtggcgg cgccagaacc tgccgagcga gctgcgtgac
      361 gaggtcgcgc ccaagaacat catcatgatc ggccccaccg gcgtcgggaa gaccgagatc
      421 gcccgccggc tggcgcggct ggcccaggcg ccgttcgtga aggtggaggc gtcgaagtac
      481 acggaggtcg gctacgtcgg gcgcgacgtc gaatcgatga tccgcgagct gaccgagctc
      541 gccgtcacca tggtcaaggc cgagatgacg gccgccgtcc aggacaaggc cgagcaggcc
      601 gcggaggagc ggctgctcga cctcctgctg ccgcgccgcg gcgagccctt ctccagcgag
      661 tcgctcgagg aggtcggcgc cggcgcgcgc gaggccaccc gcgacaagct cagggcccag
      721 ctccgcgccg gccggctgga cgaccgcatg gtggagctcg agacgcagca gcacgcgatg
      781 ccgatgatcg aggtgttctc cgggcagggc atggaggaga tgggcatcaa cctgaaggag
      841 atgctctcca acgtgctccc cacgcggacg cggcgccggc ggctccggat cggcgaggcg
      901 cggcggctcc tggtccagga ggaggcccag aagctcgtgg accacgacga ggcggtgggg
      961 ctggccatcc gccgcgtgga gaattcgggc atcgtcttca tcgacgagct ggacaaggtc
     1021 gcgggccggg aaggcggcgg ccacgggccc gaggtcagcc ggcaaggggt gcagcgggac
     1081 ctgctgccga tcgtggaggg ctcggcggtc accacgaagc acgggatcgt gcggacggat
     1141 cacatcctgt tcatcgccgc cggcgccttc cacgtggcca agccctccga cctcatcccc
     1201 gagctgcagg ggcgcttccc gatccgggtg gagctggacc ccttgacgcg cgaggatttc
     1261 gtccgcatcc tgacggagcc gcgaaacgcc ctgatccgcc agtacgtgga gctgctgctg
     1321 acggagcacg tcacgctgcg cttcctgccg gacgcggtcg aggcgatcgc ggagatcgcc
     1381 atgcaggtga acgcgagcac cgagaacatc ggcgcccgcc ggctctacac gatcttggag
     1441 aagctgctgg aggacgtctc gttcggcgcg cccgagatgc cgggcaagga gctgaccatc
     1501 gacgcggggt acgtgcacca gcggctcgcc gacatcacgc gcgaccagga cctctcgagg
     1561 tacatcctgt gacgccgccg gacgccggac aagcgcggct ggagcccatc cggcgagccg
     1621 aaatcctcat ggaggcactc ccgtacatcc gggagttccg gggcaagacc gtcgtcatca
     1681 agtacggcgg cgccgccatg gagcaggccg agctcaagga gccgtttgcc ctcgacgtga
     1741 tcctcctccg cctggtcggc atcaacgccg tcatcgttca cggcggcggg ccgcagatcg
     1801 gcgcgatgat gaagcggctg ggcaaggagc cgcgcttcgt gggtggcatg cgggtcaccg
     1861 acgcagagac ggtcgagatc gtcgagatgg tcctcgtcgg caagatcaac aaggagatcg
     1921 tgggactcat caaccaccac ggcggccgcg cggtggggct ctccggcaag gacgccgacc
     1981 tgatccgggc ccaccgccga gggcaccggt tgcccggcgg cgaggagatc gacatcggcc
     2041 tcgtcggcga ggtggacgcg gtgaatccgg agcccatccg cctgctcgag gagagcggct
     2101 tcatcccggt catcgcgccg gtcggcgtgg gcgccggggg cgagacctac aacatcaacg
     2161 ccgacctcgt ggccggcgag gtggcggccg cgctcggcgc cgcgaagctg atccacctca
     2221 ccgacgtgca gggcatcatg gacgggcagg gccggctcgt cagcacgctg gcccgccgtg
     2281 acgcggagcg gctggtgcgc gagggcgtca tcgacggagg gatgctgccc aaggtggagt
     2341 cctccctgcg tgccctcaag ggcggcaccg cgaaggccca catcatcgac gggcgccagc
     2401 cgcacgcgat cctgctcgag atgttcacac gcgagggcat cggcaccgag atcgtgctct
     2461 aggagccatg atggacacca agacgctgtt cgagtggtcg gccacctacc acatgccgaa
     2521 cttcgcccgg accccgatcg tgctcgtccg cggcgacggc ctgcgggtct gggacaccga
     2581 agggcgcgag tacctcgact tcggcgcggg cctcgcggtg accgcgctgg ggcactccca
     2641 tccgcgcgtg accggcgcca tccgcgaggc cgcggcgacg ctcctgcacg tgtcgaacct
     2701 ctaccacaac gtgccgcaga tccatctcgc caagctgctc tgcgagcact cgttcgccga
     2761 ccaggcgttt ttctcgaact ccggggccga ggccaacgag gcggccatca agatggcccg
     2821 caagtacggc aaggagcgct tcgccaccga ccgttacgag atcatcgcca cccacgactc
     2881 gttccacggc cggacgctcg ccaccgtgac cgcgaccggg cagccgaagt accaccacgg
     2941 cttcgagccc ctggtggccg gcttcaagca cgtgccctac aacgacctga agtcgatgga
     3001 gcgcgccatc gacaaccgca cggcggcgat cctggtcgag cccatccagg gcgagggcgg
     3061 cgtgcacgtg cccgacgacg actatctgcc cggtctccgg aagctctgcg acgagtcggg
     3121 cgcgctgctc atcctcgacg agatccagac gggcgtgggc cggacgggtc gcctgtgggc
     3181 ctacgagcac gccggcgtcg agccggacgt gatgaccctc gccaaggcgc tggccaacgg
     3241 cctgcccatc ggcgccacgc tggccaggcg ggaggtcgcc agcgtcatga cgccgggcac
     3301 ccacggcagc accttcggcg gcaacccgtt cgtcacctcg gtcgcgctgg cctgcatgac
     3361 gaccatgctc gacgacaaga ttcccgagcg cgccgcgcgc gtcgggcgcc acctcatcga
     3421 cgggctgcgg cggatcgcgg cggcgcggcc cgcggtgcgc acggtgcggg gcaaggggct
     3481 gctgatcggc gcggagctga cgcgcccggc ggctccggtg gtggacgcgt gccgggaggc
     3541 gggcctgctg atcctttccg cgggcgagaa ggtcgtgcgg ctggcgccac cgctcatcgt
     3601 cgacgagcgg ctctgcgacc aggccctcgg catcctggac agcgcgctcg cgaagaccgc
     3661 gtgaagcacc tcatctccgc gggcgacctc acccgcgagc gggccctgca cctgttccgg
     3721 acggccgccg cgctcaagca ggggcgcaag gcgggtacgc gcggcgcgcc gctcagcggc
     3781 cggaccctcg cgctgatctt cgagaagccc tcgctgcgca cgcgcgtgac cttcgaggtc
     3841 ggcatcgtgc agctcggagg gcgcgccgtc tacctgagcg gcgccgagat cggactgggc
     3901 acccgcgagt cggtccccga cgtcgcccgc aacctctcgc tgtgggtgga cggcgtggcc
     3961 gcgcgggtgt acgcccacgc caccgtcgag gccctcgcgc gcagcgcgac ggtcccggtg
     4021 atcaacgggt tgagcgactt cgagcatccg tgtcaggcgc tcgcggacta cttcacgctg
     4081 tgggagcggg gggtggacct cggcgccatg cggctcgcgt ggatcggcga cggcaacaac
     4141 gtcagccact cggtcgcgct gctcggcgcc ctcttcggca ccgaggtggt cgtggcctgc
     4201 ccgccgggct acgagccgaa ccccggggtc caggcgaccg tgcgtcgcct gggcggccgc
     4261 ctcagcgtgg tgcacgaccc gcgtgacgcc gctgccgggg ccgacgccat ctacacggac
     4321 gtgtggacga gcatggggca ggaggccgag cgcgagcggc ggctcgaggc cttcggccgg
     4381 ttccaggtga acgagcgcgt ggtgggcttc gccaagccgt ccgtgctggt catgcactgt
     4441 ctccccgcgc accgtggcga ggagatcacc gacgcggtgc tcgacggcgc ccggtccatc
     4501 gcgctcgcgc aatcggagaa tcgcctgcac gcccagaagg ccgtcatcct ggacctgctc
     4561 ggagggtcgc tcgacgatgc ctgattcatc gagccatttc agagcgcggc tcgcggctcc
     4621 cggcggcgga gccgccgaga gcgcctctcc ccgcgcaccg aagagagtcg tcctggccta
     4681 ctcgggcggg ctcgacacct cggtcatcct ccgctggctc atcgagcgct acgggtgcga
     4741 ggtcgtggcg ttctgcgccg acctgggaca gggggaggag ctgatccccg tccgcgacaa
     4801 ggcgctccgg accggcgccc agcgcgtgca catcgtggac ctccgagagc aattcgcgcg
     4861 cgacttcgta tttcccatgc tgcgggccgc cgccgtctac gagggcagct acctgctcgg
     4921 cacctcgatt gcgcgcccgc tcatcgccag ggcgcaggtg gagatcgcgc tggaggaagg
     4981 cgccgacgcg gtcgcccacg gggcgacggg caagggcaac gaccaggtgc gcttcgagct
     5041 gacgtacgcg gcgctggcgc cccatctgaa ggtgattgcg ccctggcgcg agtgggatct
     5101 cgactctcgc agcgcgctca tcgacttcgc gcggcggcac gacatcccgg tgccggtgac
     5161 gaccgagcgg ccctacagca cggaccgcaa cctcttccac atctcctacg aggggggcgt
     5221 cctcgaggat ccctgggcgg cgcctccggt caagatgttc ctgctcacga acgcgccgga
     5281 gcaggccccc gacgtccccg tcgaggtact gatcgacttc gaggccggca atcccgtcgc
     5341 cgtggacggc cagcggctcg ggccggtggc gctgctcgag cggctcaacc gcgtcggcgg
     5401 cgagcacggc atcggacgcg tggatctcgt ggaaaaccgc ttcgtgggca tgaagtcgcg
     5461 cggcgtgtac gagacgccgg gcgggacgat cctgcacgcc gcgcgccggg cgctggagtc
     5521 gctcacgctc gatcgcgagg tgctccacct gcgcgacggt ctcgtgcccc ggtacgccga
     5581 gatgatctac tacggcttct ggttcgctcc ggagcggcgg gtgctccaga cgctcatgga
     5641 cgagtgcgtc cgcgacgtga cggggaccgt gcgcctcacg ctgtacaagg ggaacgtcac
     5701 cgtgacgggc cggcggtcgc cccgctcgct ctaccgcacc gacttcgcga ccttcgaggc
     5761 cgacagcgtc taccgaccgg gcgacgccga gggcttcatc aacctcaacg ccctgcgact
     5821 gcggatccgc gccctccgcg accgcggacg ctgacggcgt gcgcgtcgac gtcgccccga
     5881 ccgccgaagc tttggccggc cgctccgtcg agggcgtcgt cgtcctcgtc atcgacgtgc
     5941 tccgcgccag caccaccatc gtcaccgcgc tggcgaacgg ctgtgccgcg gtcgttcccg
     6001 tcgccgatcc cgaggaggcg cggcggcggg cgagggcgct gccgccgggg acggtgctga
     6061 ccgcgggtga gcggcgcggc gaacccattc agggcttcga cctgggcaac tcgcccaccg
     6121 actgcacggc cgagcgggtg cgcgggcggg tgatcgtgtt cacgacgagc aacggaaccc
     6181 gggcgctcct ggccgcgcgc gccgcggccg ccatcggggt cgccgcgctc gtgaacgtca
     6241 gcgccgcggc gacctgggcg ctcggcgccg gccgcgacgt caccctgctg tgcgccggcg
     6301 agcggggcgc catctcgctg gaggatcacg tctgcgcggg gctgctggcc gagcggctgc
     6361 agcgcgaggc gccgtcggcg acgctcacgc cggccgccga ggcggcggtc cgggcgtcgt
     6421 gcggctacgg caaggcggtg gcccggctgg cgcaggactc gaggtgggcg cgccacctcg
     6481 cacgcaccgg ccgcggcgac gacgtcgccg cgtgcctcgt cctggacgcc gtggcgctcg
     6541 tccccgtgta tctgcccgac gttgacaagg tcgtttcggg ccgtcgataa tgccgagcgg
     6601 agcggcgtgt cacacgagat ggtgacgtga atctaccgat cggactcacg ctcacgcgga
     6661 tcgtcgccgt cccgctgctg atcgtcttcc tgatctcgtc ctcacgcgtc cacgccgtca
     6721 tcgccgccgg tatcttcatc ctcgcctcgc tgacggactg ggccgacggc gtgctcgcgc
     6781 gccggcggaa ccaggtgacg acgctgggga cgctgctgga tccgatcgcc gacaagctcc
     6841 tggtcgcagc cgcgctcgtc tcgctgctgc agatcgacaa ggtcgcggcg tggatggtgg
     6901 tcgtgatcat cggccgcgag ttcgccgtca ccgggctccg cgcggtcgcc gcgcacgtgg
     6961 gcgtcatcgt gccggcgtcc cgcctggcca agtggaagac ggcgggccag tacgtcgcca
     7021 tcaccatgct gatcatcgag aagggcgtcg gctcgccggc gttccacgtc gccgccacgg
     7081 tcgtcctgtg gatcgcgctc gcgctcacgg tggtctcggc ggtggactac ttctaccgct
     7141 tcttccggaa ggccgactac cgcgccatcg tccccgggga ggagcgctgg tcgtgagcac
     7201 cctcggggct ctggccgccg cctacctcat cggcgcgctg cccttcggct tcctcgtggc
     7261 ccgcgcctgg ggcatcgggg acatccgcca gcacggcagc ggcaacatcg ggatgacgaa
     7321 cgtgctgcgc accgccgggc gcacgcctgc gatcctcacg ctggtcggcg acgtcgcgaa
     7381 gggctacctg gccgtcgccg ccggggccgc ggtcgccggc ggcgagccgg cggcgacggc
     7441 ggcatccgcg gtcgccgcga tcgtcggcaa ctgctggtcg atcttcctcg cgttccgcgg
     7501 cggcaagggc gtggcgaccg gcctgggggc cttcctcaac ctcgcgccgc tggcggtggc
     7561 cccggccgcg ctcgtgtggc tgctcgtgac gctgagcttc cgctatgtct cgctcggctc
     7621 gctcatggcc gcgatctgcg tgccgctcgg cgcggcgctg ctcggctacg gcgccgcgaa
     7681 ggccgcggcg tgcgcagcgg gcgcggccat tgtcgtcctg cgtcaccacg agaacatcgg
     7741 gcggctcttc gccgggacgg agcgccgtct cggaggacga cgcgcgtgag gctaaccgca
     7801 tgagatgccc cgacatgggc cgatcaagac ccggggggcc tcgacatggg cctccatgcc
     7861 cctcgaggct cggcgcgccg cgacggagcc gtggcgcgcc tcggcatggc gacgtgctcc
     7921 tgggcgctcg cgccggtggc cgctgaggtg gcgcttcgca tcggcgtggt cggcggcggc
     7981 agctggggca ccgggctcgc catccacttg gcgcgggcgg gcgccgcggt gcgcctctgg
     8041 gcccgcgagg ccgaggtcgt cgagggcatc cggcgggccc gccgcaaccc gttctacctg
     8101 aaggacatcg actgcccgac cggcatcgac ccgacgggcg atcccgcaga ggtcgtgcgc
     8161 gatgccgacg tcatgctcct cgtggtgccg tcggagttct tcgcgctcac cctcaagggg
     8221 ctggggcgga tcccccgggg cgtcccgatc gtctctgcca cgaagggtct cgatccggag
     8281 cgccacgtgc gcatgagcga gatcgtcgcc gaacagcacc cggacgcgcc ggtggccgcg
     8341 ctgtcgggtc cgtcgttcgc gcgcgaggtg gccctcggcc agccgacggc ctgtgtggtc
     8401 gccgccgcgg atgacgccct ggccacgcgc ctgcaggccg cgctgggcac ccgggagttc
     8461 cggctctaca ccaaccgcga cgtcgtgggc gtcgagatgg ggggcgcact caagaacgtc
     8521 atcgcgatcg cgacgggcat cgccgacgga ctcgggctcg gcgagaacgc gcgcg
//
LOCUS       DATIAZ010000191        11494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1497557, whole genome shotgun sequence.
ACCESSION   DATIAZ010000191 DATIAZ010000000
VERSION     DATIAZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497557"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VLF19_06765"
     CDS             <1..304
                     /locus_tag="VLF19_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HSE92990.1"
                     /translation="FYSPIYTHEVVTFKAGLNHVGTSSLEVGVKVLAERPWTGEVRHA
                     CTAFLTFVHLGPDLRPRPCPPFTPQTPDERRRWEAAVVRRELRLERVKRLKASIGG"
     gene            309..1061
                     /locus_tag="VLF19_06770"
     CDS             309..1061
                     /locus_tag="VLF19_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSE92991.1"
                     /translation="MSGHSRWSQIKRKKGKADVQRGKLFSKILREITVAARNGGGDPK
                     ANMRLKAAIESAKEANMPQENIKRAIQKGTGELPGESYEEISYEGYAPGGVAVMIRVL
                     TDNRNRTAPEIRHTFEKHGGHMGASGAVAWMFERKGIVRVDAEKITEDDLLAQALDAG
                     ATDMTRAEKVFEITTAPGEMDAVRAKLEANRVPVVEAEVTLLPQSTVRVEGKEAPQVL
                     RLIEALEELDDVQAVYANYDIPDEVMEAISAA"
     gene            1096..1569
                     /gene="ruvC"
                     /locus_tag="VLF19_06775"
     CDS             1096..1569
                     /gene="ruvC"
                     /locus_tag="VLF19_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSE92992.1"
                     /translation="MLGIDPALVFTGYAVLEAGAGLSILDAGIVETNPRTSLDDRIHH
                     IYGEVWRLIDKHAPEGVALEGLYAAYSFPRTALLMAHARGVVCLAARQRGVPVLTLAP
                     AEVKRAVTGNGAAGKEQVQRSVQHLLALADLPRPSHVADALALAFTALSRRGPVR"
     gene            1566..2219
                     /gene="ruvA"
                     /locus_tag="VLF19_06780"
     CDS             1566..2219
                     /gene="ruvA"
                     /locus_tag="VLF19_06780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSE92993.1"
                     /translation="MIASLTGRLRRKDDDRIVLETGGVGYEVLLPPIVIRQLEGLKAA
                     DGDGASELRLVTHYHATRDQPRPVLIGFTSELDKEFFERLITVKDMGPMVAARALAAP
                     VGEVAAAIARQDEKYLRSLPGIGPQKAKNIVAQLHGKVAKFALARDAEAAPPAPPPAG
                     PEGADGLRELVWEVLVKQLGHRPTEASQLITEALKRRPAIVTAEDLFDEIYRGAKPS"
     gene            2216..3283
                     /gene="ruvB"
                     /locus_tag="VLF19_06785"
     CDS             2216..3283
                     /gene="ruvB"
                     /locus_tag="VLF19_06785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSE92994.1"
                     /translation="MSAAAAPTPPDDQRIARILSRAAAPEEAQFERQLRPRSFAEYVG
                     QEQAVASLRVSVEAARQRGECVDHVLLYGPPGLGKTTLAGVIANEMGTNLVTTSGPAI
                     ERGADLMGILTNLGEHDVLFVDEVHRLPRVVEELLYPAMEDFAVNFVIDRGMHARTLK
                     YALRPFTLVAATTRPGMLSAPLRERFGIFQHLDFYSEAELTRIVTRSASILGAAVDAA
                     GAAEIARRARGTPRIANRLLRRVRDYAQVAGGGAIDGAIAHDALDKEGVDGRGLDRLD
                     RRFLAAIIDHYGGGPVGIEAVAATINDEAESLTEMVEPYLLKIGLLVRTQAGRRVTRE
                     AYLHVGKQPPASPSGQAGLFE"
     gene            3280..3495
                     /locus_tag="VLF19_06790"
     CDS             3280..3495
                     /locus_tag="VLF19_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSE92995.1"
                     /translation="MSDVGKLLIALGLLIALAGLALVLAGRIPWLGRLPGDIHIERGN
                     WTFYFPLATSLLLSVVLTLVLWLIGRR"
     gene            3584..4741
                     /locus_tag="VLF19_06795"
     CDS             3584..4741
                     /locus_tag="VLF19_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HSE92996.1"
                     /translation="MSIVPQVCRPLRVTPVLVVALLATWAPEAWASGMIRVALVEAVQ
                     TVELRGAMMEVAELGGCRRCGRWRADVVRAAWTAGALEVDRRRVAGVRLRSDRPIRMS
                     GREYAAPLELVPNGGGIAVVNEVPLEEYLAGVVRAEAGEQWPTEALRAQAVVARTYAA
                     YHRRLNAGKPYHILASTAHQQYAGRVGAASPAWGAVLETTGQVLRWEGELFPAFYHTE
                     SGGYTDDPRTVFAARNMPALKPVVCPFSAGAPHFYWTLDLRLAELSEMLRRQGADIGG
                     VHAVEVSERTASLRAVTVTVRGSRGVTQLRGNDFRRIVGYDTLKSTLFAVAVDGGTAH
                     FAGRGYGHGVGLCQWGAKGMAEQGHTARQILEFYYPGTTFGVLDSRTGATR"
     gene            4802..5623
                     /locus_tag="VLF19_06800"
     CDS             4802..5623
                     /locus_tag="VLF19_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE92997.1"
                     /translation="MRLQGRVALVTGGGSGIGRAIAVRFAEEGARVVVNDLRLDAARE
                     TTDKLPGGGGRAIQADVADSGQVRAMFAEVERVYGALDVLVNNAGIAVTGGERDELAR
                     KAETRLGEVLSGQPIQTHWDVTQAMTDEAWHRMIGVHLDGTFFCTREALRLMSRAGRG
                     AVVNVSSVAALTGLDSVPHYSAAKGGILAFTRAVAREVASRGIRVNAICPGYIDTPMT
                     AGVSPLMRAAVLSRIPQGRTGAPEDVAAVALFLACDESAYLTGQWVSPNGGLFIG"
     gene            5717..6751
                     /gene="queA"
                     /locus_tag="VLF19_06805"
     CDS             5717..6751
                     /gene="queA"
                     /locus_tag="VLF19_06805"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSE92998.1"
                     /translation="MELEAFDYHLPPSAIAQAPAERREASRLLLVDRARRALADHVFA
                     DLPALLRPGDCLVVNDSRVIPARVLARDAGGRAVELLFVEPTTGGRWRALVRPGRRCR
                     PGTALTAGDDVRARLRVAAALPDGTREVERLDGTVDELLARHGLVPLPPYITRFAKPD
                     AEDAERYQTVYARSPGSIAAPTAGLHFSSAVLDGLRARGVEVHEVTVHVGPGTFRPIT
                     SPRVEDHVLPSERVSISRTVAGAVNAAKADGRRVVAVGTTTVRALEGAAGPDGRVAPL
                     EGRVDLYIAPGHRFRAIDALLTNFHLPRSSLLVLVAAFAGRDLVLDAYHHALSAGYRF
                     YSFGDACLVQ"
     gene            6748..7851
                     /gene="tgt"
                     /locus_tag="VLF19_06810"
     CDS             6748..7851
                     /gene="tgt"
                     /locus_tag="VLF19_06810"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSE92999.1"
                     /translation="MTFDLLGTDGGARRGRLTTPHGAVDTPAFMPVGTRGTVKSLAPD
                     DLRAAGAQIVLANTYHLLLRPGHDLVRELGGLHRFMAWEGPILTDSGGFQIFSLAKLR
                     KITEEGVEFRSPVDGATHFLSPEQSIEVQQALGADIIHPLDECLGYPATHEQAAASLA
                     LTLRWARRSQAAHRDAGGRQALFGIVQGGTFEDLRARAVEETVALGFDGYAIGGMAVG
                     EPKPAMYDLVELAASRLPAARPRYAMGVGKPADLVESVARGVDMFDCVLPTRNARNGQ
                     CFTAEGPVAVKHAAHARDPRPLEEDCPCYGCRHFSRAYLRHLFVSDELLAYRLLTLHN
                     VTFFLRLMAAMREAIAARAFAAFKARFFSRYPV"
     gene            7848..8219
                     /gene="yajC"
                     /locus_tag="VLF19_06815"
     CDS             7848..8219
                     /gene="yajC"
                     /locus_tag="VLF19_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSE93000.1"
                     /translation="MIQAFMNLVHAAELAYASSAPPGGQGGAGTGAVVTQVVFFAAIF
                     AIFYFLLIRPQQKQKRDRERMLGAVRKGDRVVTTSGLHGTVTGLGEHTITLRVADQVK
                     LEFDRSAIGRVVPEGQADKDA"
     gene            8212..9771
                     /gene="secD"
                     /locus_tag="VLF19_06820"
     CDS             8212..9771
                     /gene="secD"
                     /locus_tag="VLF19_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSE93001.1"
                     /translation="MPRRLWLRIGLIVLVVAGSIWYLYPPKRTVNLGLDLQGGIHLVL
                     GVEADKAIAAQTDRVAEDIKASFERKGIGVTRIAREGRAAIVVHLAKPETWNDALTAV
                     TEFGQTFERRDEDQAAGRFRLVLSQAEIDRLRDLAVRQAVETIRNRVDQFGVAEPTIT
                     RQGQDRVLIQLPGVQDPDRAKALIGKTALLEFKLLDERTSVDDALAGRLPEGSEVLYQ
                     RRLDKETKQEKRTPYVVQKRTLLTGAELNRAEVQADPNSVGGWQVSIEFNATGARVFG
                     DVTEQNVGRHLAIILDSNVYSAPRINERIPGGRAVITGQFTVEDARDLAIVLRAGALP
                     APVTILEERTVGPSLGADSIRQGLIAIAASAAIVFVFMLAYYRLSGLIADVALGLNLL
                     ILMACMAAFGATLTLPGIAGIALTIGMAVDTNILIFERIREELRVGKTPRAAIDSGFS
                     RAFRTIIDTHVTVLVTAAILYNFGTGPVKGFAVSLFVGLTASLFTAVFFTRLLFDLVY
                     MGRRRLEAISI"
     gene            9779..10747
                     /gene="secF"
                     /locus_tag="VLF19_06825"
     CDS             9779..10747
                     /gene="secF"
                     /locus_tag="VLF19_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSE93002.1"
                     /translation="MIQLFRNPNYDFIGKRRWAYVVSIVFIAIGLASIVVKGGLRYDI
                     DFAGGTLVQVRFEQPPATDRIRGALGRIGLGDSVIQEFGDPHEFIVRMPLTETKPGDV
                     TRRVQDALAAESGLGKFEIRRVEFVGPQVGRDLQLQAIYAVLAGLIGILLYIAIRFDL
                     KGGVAAVVAVFHDVLVSVGALSLTNREFSLPVLAAILTIIGYSVNDTIVAYDRLRENR
                     GRVAQRKGASFAEQINAAINQTLSRTVLTALTTFFATGILLFFGGKVLEDFAFALFVG
                     VITGTYSTIYIAGALIVEWTSYVEGRVGPGKKPVAKTREPRVKSHV"
     gene            10786..>11494
                     /locus_tag="VLF19_06830"
     CDS             10786..>11494
                     /locus_tag="VLF19_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSE93003.1"
                     /translation="MEEVPKYQPGADLDVLTRAYSFSAACHQGQQRASGEPYLSHPLE
                     VARLLVGFKMDVTTVTAGLLHDVLEDTQATKDDLEREFGKEIADLVDGVTKLGKLAFS
                     SREERQAENFRKMLVAMARDLRVLMIKLADRLHNMRTLDYLPPEKSRKVSQETLDIYA
                     PLAHRLGMAKVKAELEDLALRVLAPEDYQDLMRRVAKRRLEREADINQAIAILGQKLT
                     EVGIESQIRGRPKHFYSI"
BASE COUNT         1666 a         4111 c         4062 g         1655 t
ORIGIN      
        1 cttctactcg cccatctaca cgcacgaggt cgtcaccttc aaggccgggc tcaaccacgt
       61 cgggacgtcg tccctcgagg tcggcgtgaa ggtcctcgcc gagcggccct ggacgggcga
      121 ggtgcggcac gcgtgcacgg ccttcctgac cttcgtccat ctcggccccg atctgaggcc
      181 gcgcccgtgc ccgccattca ccccccagac gcccgacgag cgcaggcgct gggaggccgc
      241 ggtggtccgg cgcgagctgc ggctcgaacg cgtcaagcgg ctcaaggcgt cgattggagg
      301 ctaacgcgat gtcagggcat tcccggtggt cacagatcaa gcggaagaag ggcaaggcgg
      361 acgtccagcg gggcaagctc ttctcgaaga tcctgcgcga gatcaccgtc gccgcccgga
      421 acggcggcgg agacccgaag gccaacatgc ggctcaaggc ggccatcgag tcggccaagg
      481 aagccaacat gccgcaggag aacatcaagc gtgccatcca gaagggcacg ggcgagctcc
      541 ccggcgagtc ctacgaggag atcagctacg agggctacgc gccgggcggc gtggcggtga
      601 tgatccgcgt gctcacggac aaccgcaacc ggacggcgcc ggagatccgc cacacgttcg
      661 agaagcacgg cggccacatg ggcgcctcgg gcgccgtcgc ctggatgttc gagcgcaagg
      721 gcatcgtccg cgtggatgcc gagaagatca ccgaggacga cctgctcgcc caggcgctcg
      781 acgcgggcgc gacggacatg acgcgggccg aaaaagtttt cgagatcacc accgcccctg
      841 gtgaaatgga cgccgtgcgc gcgaagctcg aagcgaatcg cgtgccggtc gtcgaggccg
      901 aggtgaccct tctcccgcag tcgacggtgc gtgtcgaagg caaggaggcc ccccaggtgc
      961 tgcgcctcat cgaggcgctg gaggagctgg acgacgtcca ggcggtctac gcgaactacg
     1021 acatcccgga cgaggtcatg gaggccatct ccgcggcctg agggcggggc ggccgcggcc
     1081 ccgcgcctgc gccgtgtgct cggcatcgat cccgcgctgg tcttcaccgg ctacgcggtg
     1141 ctcgaggcag gggcggggct gtcgatcctc gacgccggca tcgtcgagac gaatccccgg
     1201 acgtcgctcg acgaccggat ccatcacatc tacggcgagg tctggcgcct gatcgacaag
     1261 cacgcgccgg agggcgtggc gctcgagggc ctctacgcgg cgtacagctt cccgcggacg
     1321 gcgctcctca tggcgcacgc ccggggcgtc gtgtgcctcg cggcgcgcca gcgcggcgtg
     1381 cccgtgctca cgctcgcccc ggcggaggtc aagcgcgccg tcaccggcaa cggcgccgcc
     1441 ggcaaggagc aggtgcagcg gagcgtgcag cacctgctgg cgctcgcgga tctgccgcgg
     1501 ccctcccacg tcgccgacgc gctcgcgctg gcgttcacgg cgctgtcgcg gcggggcccg
     1561 gtgcgatgat cgcgtcgttg accggccggc tgcggcgcaa ggacgatgac cgcatcgtgc
     1621 tcgagacggg cggcgtgggc tacgaggtgc tgctgccgcc gatcgtgatc cggcagctcg
     1681 aggggctgaa ggccgccgac ggcgacggcg ccagcgagct acgcctggtg acgcactacc
     1741 acgcgacccg ggaccagccg cggcccgtcc tcatcggctt cacgtcggag ctcgacaagg
     1801 agttcttcga gcggctcatc accgtgaagg acatgggccc gatggtcgcc gcgcgggcgc
     1861 tggcggcgcc ggtcggcgag gtggccgccg ccatcgcccg ccaggacgag aagtatctgc
     1921 ggtcgctacc cgggatcggg ccgcagaagg ccaagaacat cgtcgcgcag ctccacggca
     1981 aggtcgcgaa gttcgcgctg gcccgcgacg cggaagctgc gccgccggcg ccgcccccag
     2041 ccggacccga gggtgccgac ggcctgcggg agctggtgtg ggaggtcctg gtgaagcagc
     2101 tcggccaccg cccgaccgag gcctctcagc tcatcacgga ggcactcaag cggcggccgg
     2161 cgatcgtcac cgccgaggac ctgttcgacg agatctaccg cggggcgaag ccctcgtgag
     2221 cgccgcggcg gcgcccacgc ctccggacga tcagcggatc gcgcgcatcc tcagccgcgc
     2281 ggcggcgccg gaggaagccc agttcgagcg ccagctgcgg ccgcgctcct tcgccgagta
     2341 cgtgggccag gaacaggcgg tggcgagcct gcgggtctcg gtggaggccg cccggcagcg
     2401 cggcgagtgc gtggaccacg tgctactcta cggcccgccg ggcctcggca agaccacgct
     2461 cgccggcgtc atcgccaacg agatggggac gaacctcgtg accacgtccg gcccggccat
     2521 cgagcgcggg gccgacctca tgggcatcct caccaatctc ggcgagcacg acgtgctctt
     2581 cgtggacgag gtccaccgcc tgccccgcgt cgtcgaggag ctgctgtatc cggccatgga
     2641 ggacttcgcc gtcaacttcg tcatcgaccg cggcatgcac gcccgcacgc tcaagtacgc
     2701 gctgcggccc ttcaccctcg tggcggcgac cacgcggccg ggcatgctgt cggcgccatt
     2761 gcgcgagcgc ttcggaatct tccagcacct ggacttctac tccgaggccg agctcacgcg
     2821 catcgtcacg cgctcggcct ccatcctggg cgccgccgtg gacgccgccg gcgccgcgga
     2881 gatcgcgcgc cgcgcgcgcg gtacgccgcg catcgccaac cgcctgttgc gcagggtccg
     2941 ggactacgcc caggtcgccg gtggcggcgc catcgacggc gccatcgcgc acgacgcgct
     3001 cgacaaggaa ggggtggatg gccgtggtct cgaccgtctc gaccgccgct tcctcgcggc
     3061 gatcatcgat cactacggcg gcgggccggt cggcatcgag gcggtggccg cgacgatcaa
     3121 cgacgaggcc gaatcgctca ccgagatggt ggagccctac ctgctgaaga tcggtctcct
     3181 cgtccgcacc caggccggcc gccgcgtgac acgggaggcg tacctgcacg tgggcaagca
     3241 gccgccggcg tcgccgtcag ggcaggcagg gctctttgag tgagcgacgt cggcaagctc
     3301 ctgatcgccc tcgggctgct catcgccctg gccggtctgg ccctcgtgct cgcggggcgg
     3361 atcccctggc tgggacgcct gccgggcgac atccacatcg agcgcgggaa ctggaccttc
     3421 tatttccccc tcgcaacgag cctgctgctg agcgtcgtgc tcacgctcgt cctgtggctg
     3481 atcgggcgac gctgagaccc gggagggggc tccagtggcc cctcccgacc accccagaac
     3541 cggtggcgca ggcgaaggct cgcgctcgaa cggagggcat cgagtgagca tcgtcccgca
     3601 ggtgtgccgc ccgcttcgcg tgactcctgt gctcgtcgtg gcgctgctcg ccacctgggc
     3661 gccggaggcg tgggcgtcgg ggatgatccg tgtggcactc gtggaggcgg tgcagacggt
     3721 ggagctgcgc ggcgccatga tggaggtcgc ggagctcggc ggctgcagac gctgcgggcg
     3781 ctggcgcgcc gacgtcgtgc gcgcggcgtg gacggccggt gcgctcgagg tcgaccgccg
     3841 gcgcgtcgcg ggcgtgcgac tgcgcagcga ccgcccgatc cgcatgagcg ggcgggagta
     3901 cgccgcgccg ctcgagctcg tgcccaacgg cggcggcatc gccgtggtca acgaggtacc
     3961 gctcgaggag tacctggccg gcgtcgtccg ggcagaggct ggcgagcaat ggcccaccga
     4021 ggcgctgcgg gcccaggccg tggtcgcccg cacctacgcc gcataccacc ggcggctcaa
     4081 cgcgggcaag ccctaccaca tcctcgcctc caccgcgcac cagcagtacg ccggccgcgt
     4141 cggcgccgcc tcgcctgcct ggggcgccgt gctcgagacg accgggcagg tgctgcgctg
     4201 ggagggcgag ctgttccccg ccttctatca cacggagagc ggtggctaca cggacgaccc
     4261 ccggacggtg ttcgccgcgc gcaacatgcc ggcgttgaag cctgtggtgt gcccattctc
     4321 ggcgggcgcg ccgcacttct actggaccct cgatctgagg ctcgccgagc tgtcggagat
     4381 gctgcggcgc cagggcgccg acatcggcgg ggtgcacgct gtcgaggtca gcgagcgcac
     4441 ggcgtcgctg cgcgcggtca cggtgacggt gcgcggcagc cggggcgtca cgcagctgcg
     4501 cggcaacgac ttccgccgca tcgtcggcta cgacacgctc aagagcacgc tgttcgcggt
     4561 cgccgtggac ggcgggacgg cccacttcgc gggccgcggc tacggccacg gggtggggct
     4621 ctgccagtgg ggcgccaagg ggatggccga gcagggccac accgcccgcc agatcctgga
     4681 gttctactac ccgggcacca cgttcggggt cctcgattcc aggacggggg ccacgaggtg
     4741 atcctcctgc acctcgacca gacgatcgtc ccgggcgcga agatcgagta gagtccccgc
     4801 cgtgaggctg caaggtcgcg tcgcgctcgt caccggcggt ggctccggca tcggccgggc
     4861 catcgcggtg cgcttcgccg aggagggcgc gcgcgtcgtc gtgaacgacc tccggctcga
     4921 cgccgcgcgg gagaccaccg acaagcttcc cggcggcggc gggcgcgcca tccaggccga
     4981 cgtcgccgac agcgggcagg tgcgcgcgat gttcgccgag gtcgagcgcg tgtacggcgc
     5041 cctcgacgtt ctcgtcaata acgccggcat cgcggtgacc gggggcgagc gcgacgagct
     5101 cgcccgcaag gccgagacgc gcctgggcga ggtgctgagc gggcagccca tccagacgca
     5161 ctgggacgtc acccaggcga tgaccgacga ggcctggcac cggatgatcg gcgtgcacct
     5221 ggacggcacg ttcttctgca cccgggaggc gctgcgcctg atgagccggg ccggccgggg
     5281 cgccgtcgtc aacgtgtctt cggtcgctgc cctgacgggg ctggacagcg tgccgcacta
     5341 cagcgcggcc aagggcggca tcctggcgtt tacccgcgcg gtcgcccgcg aggtggcctc
     5401 gcgcggcatc cgcgtgaacg ccatctgccc cggctacatc gacacgccca tgacggcagg
     5461 cgtctcgccg ctgatgcgcg cggccgtgct gtcgcggatc ccgcaggggc ggaccggcgc
     5521 acccgaggac gtggccgccg tggcgctctt cctggcctgc gacgagagcg catacctcac
     5581 cggccagtgg gtgtcgccga atggtggact cttcatcggc tgagagcgtg taaggtcaaa
     5641 gagcgcgcca cagcttcgca gggggcgtgc cgaacgctgg gagcttgggg gcaatgtcgg
     5701 cgcccgcgtg ttactgatgg agctggaggc cttcgactac cacctgccgc cgtccgccat
     5761 cgcccaggcg ccggccgagc gccgcgaggc atcgcgcctg ctcctggtcg accgggcccg
     5821 gcgggcgctc gcagaccacg tcttcgcgga cctgcccgcg ctgctgcggc cgggcgactg
     5881 cctggtcgtc aacgactcgc gcgtgatccc cgcgcgggtg ctcgcgcgcg atgccggcgg
     5941 acgggccgtg gagctgctgt tcgtggagcc caccaccggc gggcgctggc gcgcgctcgt
     6001 ccgccccggt cggcgctgcc gccccggcac ggccctcacg gcgggcgacg acgtgcgcgc
     6061 gcggctgcgg gtcgccgccg cgctgcccga cggcacgcgg gaggtcgagc ggctcgacgg
     6121 aacggtcgac gagctcctgg cgcgccacgg gctcgtgccg ctgccaccct acatcacgcg
     6181 cttcgcgaaa cccgatgccg aggacgcgga gcgctaccag acggtgtacg cgcggagtcc
     6241 gggctcgatc gccgcgccca cggccgggct gcacttttca tccgcggtgc tcgacggcct
     6301 ccgtgcccgc ggcgtcgagg tgcacgaggt gaccgtgcac gtgggacccg ggacgttccg
     6361 cccgatcacg agcccgcggg tggaagacca cgtgctgccg tcggagcgcg tgtcgatctc
     6421 ccggaccgtc gcgggcgcgg tgaacgccgc gaaggcggat ggccggcgcg tggttgccgt
     6481 gggcaccacg accgtgcggg cgctcgaggg cgccgccggc ccggacgggc gagtggcgcc
     6541 gctggaggga cgcgtcgacc tctacatcgc gccgggccac cgcttccggg ccatcgacgc
     6601 gctgctgacg aacttccacc tgccgcgctc gtcgctgctc gtcctggtcg ccgccttcgc
     6661 ggggcgggac ctcgtgctcg acgcctacca ccacgcgctg tccgccggct accgcttcta
     6721 ctccttcggc gacgcctgcc tcgttcaatg accttcgacc tgctcggcac ggacggcggc
     6781 gcgcggcgcg gccggctgac gacgccccac ggcgcggtgg acacgccggc gttcatgccc
     6841 gtgggcacgc gaggcaccgt caagagcctc gccccggacg acctccgggc cgcgggcgcc
     6901 cagatcgtcc tggcgaacac gtatcacctg ctcctgcgtc cgggccacga cctcgtgcgc
     6961 gagctcggcg gcctgcaccg gttcatggcg tgggaggggc cgatcctgac ggactccggc
     7021 ggcttccaga tcttcagcct ggcgaagctg cgcaagatca ccgaggaggg cgtcgagttc
     7081 cgctcgccgg tggacggtgc cacgcacttc ctctcgccgg aacagtcgat cgaggttcag
     7141 caggccctcg gcgccgacat catccacccc ctcgacgagt gtctcggcta cccggccacg
     7201 cacgagcagg ccgcggcgtc gctggcgctg acgctgcggt gggcccgccg ctcccaggcg
     7261 gcgcaccgcg acgccggcgg gcgccaggcg ctcttcggca tcgtccaggg ggggacgttc
     7321 gaggacctgc gcgcccgcgc cgtggaagag accgtcgcgc tcggcttcga cggctacgcg
     7381 atcggcggga tggccgtggg cgagccgaag cccgccatgt acgacctcgt ggagctcgcc
     7441 gcctcccgac tgcccgcggc ccgcccgcgg tacgccatgg gcgtcggcaa gccggcggac
     7501 ctcgtggaat cggtggcccg cggcgtcgac atgttcgatt gcgtgctgcc cacgcgcaac
     7561 gcccgcaacg gtcagtgctt cacggccgag gggcccgtcg ccgtcaagca cgccgcccac
     7621 gcgcgcgatc cacgcccgct ggaggaggac tgcccgtgtt acggctgccg gcacttctcg
     7681 cgcgcgtatc tcaggcatct gttcgtgtcg gacgagctgc tcgcctatcg cctgctcacg
     7741 ctgcacaacg tgacgttctt cctccgcctc atggcggcca tgcgcgaggc gatcgcggcg
     7801 cgcgccttcg cggcattcaa ggctcggttt ttctcgcgat atcctgtatg atccaggctt
     7861 tcatgaacct cgttcacgcg gccgagctcg cgtacgcgtc ctccgcgccg ccgggcggtc
     7921 agggcggggc tggcaccggt gccgtggtca cccaggtcgt cttcttcgcg gcgatcttcg
     7981 cgatcttcta cttcctcctc atccggccgc agcagaagca gaagcgcgac cgcgagcgca
     8041 tgctcggcgc cgtcaggaag ggtgaccgcg tggtcaccac gagcgggctg cacggcaccg
     8101 tcaccggcct cggcgagcac acgatcacgc tgcgcgtcgc cgaccaggtg aagctcgagt
     8161 tcgaccgttc ggccatcggg cgcgtcgtcc cggagggtca ggccgacaaa gatgcctaga
     8221 cgtctgtggc tgcgcatcgg cctcatcgtc ctcgtagtgg cgggctcgat ctggtacctg
     8281 tatccgccga agagaaccgt caacctgggc ctggatctgc agggcggcat ccacctcgtc
     8341 ctcggcgtgg aggccgacaa ggccatcgcg gcccagaccg accgggtcgc cgaggacatc
     8401 aaggcgtcgt tcgagcgcaa ggggatcggc gtgacccgga tcgcgcgcga gggccgcgcc
     8461 gccatcgtcg tccacctcgc gaagcccgag acgtggaacg acgcgctcac cgccgtcacc
     8521 gagttcggcc agacgttcga gcggcgcgac gaggaccagg ccgccggccg cttccgcctc
     8581 gtgttgagcc aggccgagat cgaccgtctc cgtgacctgg ccgtgcgcca ggccgtggag
     8641 acgatccgca accgcgtcga ccagttcggg gtggccgagc ccaccatcac ccgccagggc
     8701 caggaccgcg tcctgatcca gctccccggg gtgcaggacc ccgaccgggc caaggcgctg
     8761 atcggcaaga cggccctgct cgagttcaag ctcctcgacg agcggacgtc ggtggacgac
     8821 gccctggccg gccggctgcc cgaggggtcg gaggtgctct accagcggcg cctcgacaag
     8881 gagacgaagc aggagaagcg gacgccctac gtcgtccaga agcgcacgct cctcaccggc
     8941 gccgagctca atcgcgcgga ggtgcaggcc gacccgaact cggtgggcgg ctggcaggtc
     9001 tcgatcgagt tcaacgcgac cggcgcccgc gtcttcggcg acgtcaccga gcagaacgtg
     9061 ggccggcacc tcgccatcat cctcgacagc aacgtgtact cggcgccccg catcaacgag
     9121 cggatccccg gcggccgcgc cgtgatcacg ggacagttca cggtggagga cgcgcgcgac
     9181 ctcgccatcg tgctgcgcgc gggcgccttg ccggcgccgg tgacgatcct cgaggagcgc
     9241 accgtcgggc cgtcgctggg cgccgactcg atccgccagg gcctcatcgc catcgccgcc
     9301 tccgcggcca tcgtcttcgt cttcatgctg gcgtactacc ggctctcggg gctcatcgcc
     9361 gacgtcgcgc tggggttgaa cctcctgatc ctcatggcct gcatggccgc cttcggggcc
     9421 acgctgacgc tgcccggcat cgccggcatc gcgctcacga tcggcatggc ggtggacacg
     9481 aacatcctga tcttcgagcg gatccgcgag gagctacggg tcggcaagac gccccgcgcc
     9541 gcgatcgact cggggtttag ccgggcgttc cggacgatca tcgacacgca cgtcacggtg
     9601 ctggtgacgg cggccatcct ctacaacttc ggcacgggtc cagtgaaggg gttcgcggtc
     9661 tcgctgttcg tgggcctgac ggcgagcctg ttcaccgccg tcttcttcac gcggctgctc
     9721 ttcgacctcg tctacatggg ccggcggagg ctcgaggcca tctcgatatg acacggcgat
     9781 gatccagctc ttccgcaatc ccaactacga cttcatcggt aagcggcgct gggcgtacgt
     9841 cgtctcgatc gtcttcatcg ccatcggcct ggcgtcgatc gtcgtcaagg gggggctccg
     9901 ctacgacatc gacttcgcgg gcggcacgct ggtgcaggtg cggttcgagc agccgccggc
     9961 gaccgatcgc atccgggggg cgctgggccg gatcggtctc ggggacagcg tcatccagga
    10021 gttcggcgac ccgcacgagt tcatcgtccg catgccgctg accgagacga aaccgggcga
    10081 cgtcaccagg cgcgtgcagg acgcgctggc cgccgagagc ggcctcggca agttcgagat
    10141 ccgccgggtg gagttcgtcg ggccccaggt gggccgcgat ctccagctgc aggccatcta
    10201 cgcggtgctg gcgggcctga tcggcatcct gctgtacatc gcgatccggt tcgacctcaa
    10261 gggcggggtg gccgccgtgg tcgccgtgtt ccacgacgtc ctggtgtccg tcggcgcgct
    10321 ctcgctgacg aaccgggagt tctcgctgcc cgtgctcgca gccatcctga cgatcatcgg
    10381 ctactcggtc aacgacacca tcgtggccta cgatcggctc cgggagaacc gcgggcgcgt
    10441 tgcacagcgg aagggcgcga gcttcgccga gcagatcaac gcggccatca accagacgct
    10501 gtcgcgcacg gtgctgacgg ccctcaccac gttcttcgcc accgggatcc tcctcttctt
    10561 cggcggcaag gtgctcgagg acttcgcctt cgccctcttc gtcggggtca tcacgggcac
    10621 gtactcgacg atctacatcg cgggcgcgtt gatcgtcgaa tggaccagct acgtcgaggg
    10681 acgcgtcggg cccggcaaaa aaccagtcgc caagacccgg gagccgcgtg taaaatcaca
    10741 tgtttaaccg gaggattcga cgcggtgacc gagctttcga cgctgatgga ggaggtgccg
    10801 aagtaccagc caggcgctga cctggacgtc ctgacgcgcg cttacagctt ctccgccgcc
    10861 tgccaccagg gccagcagcg cgcctccggc gagccgtacc tctcccatcc cctcgaggtc
    10921 gcccgcctcc tcgtcggctt caagatggac gtgaccacgg tcacggccgg gttgctccac
    10981 gacgtcctcg aggacacgca ggccaccaag gacgacctcg agcgggagtt cggcaaggag
    11041 atcgccgacc tcgtggacgg cgtcaccaag ctcggcaagc tggccttctc ctcgcgcgag
    11101 gagcggcagg cggagaattt ccgcaagatg ctggtggcga tggcgcgcga cctgcgcgtg
    11161 ctgatgatca agctcgccga ccggctgcac aacatgcgca cgctggacta cctgccgccg
    11221 gagaagtcgc gcaaggtgtc ccaggagacg ctcgacatct acgccccgct cgcccatcgc
    11281 ctcggcatgg cgaaggtgaa ggcggagctg gaggacctgg cgctgcgcgt cctggcgcca
    11341 gaggactacc aggacctcat gcgccgcgtg gccaagcgcc ggctggagcg cgaggccgac
    11401 atcaatcagg ccatcgccat cctggggcag aagctgacgg aagtcgggat cgagtcgcag
    11461 atccgcggcc gcccgaagca cttctactcg atct
//
LOCUS       DATIAZ010000192         6177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1234616, whole genome shotgun sequence.
ACCESSION   DATIAZ010000192 DATIAZ010000000
VERSION     DATIAZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234616"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..885)
                     /gene="ispE"
                     /locus_tag="VLF19_06835"
     CDS             complement(37..885)
                     /gene="ispE"
                     /locus_tag="VLF19_06835"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HSE93004.1"
                     /translation="MLRAAAKVNLVLEVLGRRADGYHEIATVMQTVDLSDRVILEDAD
                     TLDCRTTVAGLPTDGSNVAVRAARALAEAAGVARGARVTLDKRIPVAAGLGGGSADAA
                     ATLVGLNRLWGLRWPVARLGEIAVTLGMDVPFFLRGGTALATGRGEKLEPLPGVGLAL
                     VLVNPRFGSSTAEVYGRVTPEMYTDGARARAMLGALRSRRPTRVAGGLYNGLEAAAAS
                     AHAEIGRMKAGLLAAGALGTALSGSGPTVFGVARSFEHARQIRARLVRGSWECWAVRT
                     TCGRVR"
     gene            complement(949..1728)
                     /locus_tag="VLF19_06840"
     CDS             complement(949..1728)
                     /locus_tag="VLF19_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93005.1"
                     /translation="MSAPTFRGSSALLLLAAALLPVACVRAIAPPRAAISEDARRAVA
                     LLAGRWHGFSDLRTLADIRIERQGERQQLVGVLLARAPASVRLEALSPLGQPLLLATI
                     HEGQLTAYNAASNAALVGPATAETAARLLSLPFEPDDLVGVLAGRPAPPRDLRTAEVL
                     PADDQGPSLDLIGAIHRQRVWMDFASGVVRQVEITGGRYAVRVTYLLGADSRPLGFDL
                     SAGTGHLSGAVRYRDPVYDAGVDLARFSLTLPERAKIEQLR"
     gene            complement(1703..3895)
                     /locus_tag="VLF19_06845"
     CDS             complement(1703..3895)
                     /locus_tag="VLF19_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HSE93006.1"
                     /translation="MKSSSPRAVRDLLTSAVPELRERLAEDALRRGWAAVVGPDAARR
                     TRPRRLVNGCLEIVVDNSPWLSELTLRAPDLTARLGARFDGVRTLRFVVGALPADPPA
                     PGDRKPRPPPLSAEAARDIEAAVAPLADDELRAAARRLLTTARRYGTALAVLLLAGGL
                     AGGCAGTTTAAARGDDEPVRQAQPARPSDAAEAYYHYSVAQLHAQAGRFRDAIAAAEE
                     AIKHDAHSAFLWRELAQWLARAEQPEQAVAAARRAVTLAPDDPASHLALAELLRAQKK
                     YADAEAELEKVIALNPAAEEPYLTLARYYVEQKSYERAREVLLRLAERHPRLAQAQFL
                     LGRLAIETESFDEAVARLTKAVELDPDHDGAWTALGYAFEAKHAPDKAVEIYRRAVNA
                     NPDNPAFVERLGDLLIRLGRFKEAETEVEALAQAAPRDARLWMKLGAVYYEQKRWDKA
                     SDAFRRAVSLEPNNLRARYFLATSLMDGGRDDDARTELERILRVDPRSVDARVQLAFL
                     HGRAKRYDQSIALLREAVNLEPRRPELFLYLGTGYFRAKEYDRAAEALREGLTLDDKH
                     RDLHFQLGVVYEKQQRFDDAIGQFRRVIALDPKHAEAYNYVGYMYAERGQNLDEAATL
                     IQRALTLEPENGYFIDSLGWVFYQQGRYADALRELKRAVDKAKEPDPVIFDHLGDAYA
                     KNGLHEDALAAWEKSLQLDPTADGVKKKIQELRDRLGRVKGERSDLSR"
     gene            3981..5345
                     /gene="rlmD"
                     /locus_tag="VLF19_06850"
     CDS             3981..5345
                     /gene="rlmD"
                     /locus_tag="VLF19_06850"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HSE93007.1"
                     /translation="MAKPRRGDVLELPIDDLAFGGEGVGRVDGYVLFVRGGVPGDRLR
                     VRVTETRARHGRGVIEAVETSSPARVEPPCPYFGRCGGCRLQHVAYEAQLAFKEKQVR
                     DCLERLGAAGAFELRSILPAPAAYGYRNKMEFTVAGTTGAPVIGLHAADRYDVVLDIE
                     RCLLQSDTMNELLDEVRGQVRARELSVYDQASAQGLLRFVALREGRRTGDAMVNVVAA
                     APDVETLGPVAEALRARAPATSSVVLTVNAKKAAVAVGPEEHVLLGRDHIVESLGGVR
                     FEVSAGSFFQTNTLQAERLFALVAAACELGGGETVVDLYSGTGAISLLLAQRCRQVYG
                     IEVAPAAVADAVRNARENGIENCTFLAGEVRHVLPALVRDGVRADIVVADPPRAGFHP
                     KALGALVALAPARLVYVSCNPATLARDVGDLLRQGYRLEWVQPVDMFPQTPHIEAVAR
                     LRRA"
     gene            5357..>6177
                     /locus_tag="VLF19_06855"
     CDS             5357..>6177
                     /locus_tag="VLF19_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93008.1"
                     /translation="MRVYLARRLLQSLLVLLGVSFVVFAILFLTGDPALVLLPPDASA
                     ADVQAFRERMGFNDPFFVQYWRFLRGALRGDFGESVRHGEPAFPLVVERLPATLELSG
                     AALLIALALAIPAGIVSAVRRNSMVDYVSTVTALLGQSMPTFWLGIMLILVFSVWLNL
                     LPSSGRGGLAHLAMPAITLGLFTTARIMRLTRSGMLEVLGQDYIRTARAKGVAGAPVV
                     WKHALKNAAIPIVTIVGIELGTLLGGAVITETIFAWPGVGRLSVQAIYNRDYPVV"
BASE COUNT          815 a         2227 c         2234 g          901 t
ORIGIN      
        1 gccacttcac cgtccggctc gccgcccgag cgcggctcac ctcacacggc cacacgtcgt
       61 gcgcaccgcc cagcactccc aggatccgcg taccaggcgc gcgcgaatct ggcgcgcgtg
      121 ttcgaaggag cgcgcgacgc cgaacaccgt gggcccgctg cccgacagcg cagtacccag
      181 cgcgccggca gcgagcagcc cggccttcat acgcccgatc tcggcgtgtg ccgacgccgc
      241 ggcggcctcg agaccattgt aaaggcctcc ggccacccgc gtggggcgcc ggctgcggag
      301 cgcaccgagc atcgcccggg cgcgcgcccc gtccgtgtac atctccggcg tgacccgtcc
      361 gtagacctcg gcggtgctcg acccgaatcg cgggttcacc agcacgaggg cgagcccgac
      421 gccaggcagc ggctccaact tctctcctcg cccggtcgcg agtgccgtac cgccccggag
      481 gaagaacggc acgtccatcc cgagcgtgac cgcgatctcg ccgagacggg ccaccggcca
      541 gcgcaggccc cacagccggt tcaggccgac gagggtcgcc gccgcgtcgg ccgatccgcc
      601 accgagcccc gccgcgaccg ggatccgctt gtcgagcgtg acgcgtgcgc cgcgcgcgac
      661 accggccgcc tcggccagtg cccgggcggc ccgaaccgcc acgttgctac cgtccgtcgg
      721 caagccggcg accgtcgtgc ggcagtcgag ggtgtcggcg tcctccagga tcacccggtc
      781 agacaggtcc acggtctgca tgaccgtggc gatctcgtgg tatccgtcgg cgcgccggcc
      841 gaggacctcg agcacgagat tcaccttggc ggcggcgcgc agcaccaggc gacatcgacc
      901 cgaccccctc gacaagacag cgtaccgtag cacgcccacc ggggagcgtc aacgtaactg
      961 ctcgattttg gcccgttccg ggagcgtcag ggagaagcgc gcgagatcga cgcctgcgtc
     1021 gtagacgggg tcccggtagc gcacggcgcc cgacaggtgg cccgtcccgg cggatagatc
     1081 gaagccgagc ggcctgctgt ccgctccgag gagatacgtc acacggaccg cgtatcgacc
     1141 cccggtgatc tcgacctgac ggacgacgcc gctggcgaag tccatccaga cgcgctggcg
     1201 atggatcgcg ccgatgaggt cgagcgacgg gccctggtcg tcggccggaa gcacctcggc
     1261 tgtcctgagg tccctgggag gcgccggccg gccggccagc actccgacga gatcgtccgg
     1321 ctcgaacggc aggctcagca agcgcgcggc tgtctccgca gtcgcgggcc cgacgagcgc
     1381 ggcgttggat gcggcgttgt acgccgtgag ctgcccctcg tggatcgtcg ccagcagcaa
     1441 gggctggccg agcggcgaga gcgcctcgag cctcaccgag gccggcgcgc gggcgaggag
     1501 cacgccgacc agctgctggc gctcgccctg tcgctcgatc cggatgtcgg ccagcgtgcg
     1561 gagatcggag aacccgtgcc agcgcccggc gagcagggcg accgcccggc gcgcgtcctc
     1621 ggagatggcc gcacgcggag gcgcgatcgc gcgcacgcac gcgacgggga gcagggcggc
     1681 ggcgaggagg aggagggccg agctaccgcg aaaggtcgga gcgctcaccc ttgacccgcc
     1741 cgagacgatc ccggagctcc tggatcttct tcttcacccc gtcggcggtc gggtcgagct
     1801 gcagcgactt ctcccaggcg gcgagcgcgt cctcgtgcag gccgttcttg gcgtaggcgt
     1861 cgcccaggtg gtcgaagatc accgggtcgg gctccttggc cttgtcgacg gcgcgcttga
     1921 gctcgcggag cgcatcggcg taacgaccct gctggtagaa gacccacccg aggctgtcga
     1981 tgaaatagcc gttctccggc tcgagggtca aggcccgctg gatgagcgtg gccgcctcgt
     2041 cgagattctg gccgcgctcg gcgtacatgt agccgacgta gttgtaggcc tcggcgtgct
     2101 tgggatcgag cgcgatgacg cgacggaact ggccgatggc gtcgtcgaag cgctgctgct
     2161 tctcgtagac gacgccgagc tggaagtgca ggtcccggtg cttgtcgtcg agggtgaggc
     2221 cctcgcgcag cgcctccgcg gcccggtcgt actccttggc acggaagtat cccgtgccga
     2281 ggtagaggaa gagctccggc cgccggggct cgaggttcac cgcctcgcgc agcagcgcga
     2341 tcgactggtc gtagcgcttg gcgcgaccgt ggaggaaggc gagttgcacg cgggcatcga
     2401 ccgaccgcgg gtcgaccctc aagatccgct ccagctccgt gcgggcgtcg tcgtcgcggc
     2461 cgccgtccat gaggctcgtg gccaggaagt agcgtgcgcg caggttgttg ggctcgagcg
     2521 acacggcacg ccggaacgcg tcgctcgcct tgtcccagcg cttctgctcg tagtagacgg
     2581 cgccgagctt catccacagg cgcgcgtcac ggggcgctgc ctgcgcgagc gcctcgacct
     2641 ccgtctcggc ctccttgaag cgcccgagcc ggatcaacag gtcgcccagc cgctccacga
     2701 acgcggggtt gtcgggattc gcgttgacgg cgcgccggta gatctcgacc gccttgtccg
     2761 gcgcgtgctt ggcctcgaac gcgtagccga gcgccgtcca ggcgccgtcg tggtcgggat
     2821 ccagctccac ggccttcgtg agccgcgcga ccgcctcgtc gaagctctcg gtctcgatgg
     2881 ccagccggcc gagcaggaac tgtgcctgag cgagacgggg atggcgctcg gcgagccgca
     2941 gcaggacctc gcgcgcccgc tcgtagctct tctgctcgac gtagtagcgc gccagcgtga
     3001 ggtagggctc ctcggccgcg gggttcagcg cgatgacctt ctcgagctcg gcctcggcat
     3061 ccgcgtactt cttctgcgcc cgcagcagct cggccagcgc gaggtgactg gccgggtcgt
     3121 ccggcgccag cgtcaccgct cggcgcgcgg cggcgacggc ctgctccggc tgctcggcgc
     3181 gcgccagcca ctgcgccagc tcgcgccaca ggaacgccga gtgggcgtcg tgcttgatcg
     3241 cctcctcggc cgccgcgatg gcgtcgcgga accgtccggc ctgggcgtgg agctgcgcga
     3301 cgctgtagtg gtagtaggcc tccgcggcat cgctggggcg ggcaggctgc gcctggcgaa
     3361 ccggctcgtc gtcaccgcgc gccgccgcgg tcgtcgtgcc cgcgcacccg ccggcgagcc
     3421 cgcccgccag caggaggacg gcgagcgctg tcccgtatcg ccgcgccgtg gtcaagagcc
     3481 ggcgcgccgc cgcccgcagc tcgtcgtcgg cgaggggagc gacggcggcc tcgatgtcgc
     3541 gcgcggcttc cgctgacagc ggcggcgggc gcggcttgcg gtcgccaggg gccggcgggt
     3601 cagcgggcag ggcccccacg acgaagcgca gcgtgcgcac gccgtcgaag cgtgcgccca
     3661 gccgcgccgt caggtcgggc gcgcgcagcg tgagctcgga gagccacggc gagttgtcga
     3721 cgacgatctc gaggcacccg ttgacgagac gccgcgggcg cgtgcggcgg gcggcgtcgg
     3781 gtccgaccac ggcggcccag ccccgacgga gggcgtcttc tgcgagccgc tcccgcagct
     3841 cgggcacggc actcgtgagc agatcgcgga ccgcacgtgg gctcgatgac ttcacaggaa
     3901 actatgatac ctccgccgac tagccctgcg agcccgtcaa attgactcgt tccgacccgg
     3961 ccattatagt ggcctcgcac atggcgaaac cgcgtcgcgg ggacgtcctc gagctgccca
     4021 tcgacgacct cgcgttcggc ggtgagggcg tcgggcgcgt cgacggttac gtcctgttcg
     4081 tgcgcggcgg ggtgccgggc gaccgcctgc gcgtccgcgt gacggagacg cgggcgcgcc
     4141 acggccgcgg cgtgatcgag gcggtcgaga cgtcctcgcc cgcgcgcgtc gagccgccct
     4201 gcccgtactt cgggcgatgc gggggctgcc ggctgcagca cgtggcctac gaggcgcagc
     4261 tcgcgttcaa ggagaagcag gtgcgcgact gtctcgagcg ccttggcgcc gccggggcct
     4321 tcgagctgcg gtccatcctg cccgcgcccg cggcctacgg ctaccggaac aagatggagt
     4381 tcaccgtcgc cggcacgacc ggcgcgcccg tgatcggact gcacgcggcg gaccgctacg
     4441 acgtcgtgct cgacatcgag cgctgtctgt tgcagtcgga caccatgaac gagctgctcg
     4501 acgaggtccg cggccaggtg cgagcgcgag agctctccgt gtacgaccag gcctccgccc
     4561 agggcctcct gcggttcgtg gcgctgcgcg agggccgccg gaccggcgat gcgatggtga
     4621 acgtggtggc ggcggcgccc gacgtcgaga cgctcggtcc ggtggccgag gcgttgcggg
     4681 cgcgcgcacc cgcgacctcc agcgtcgtgc tcaccgtcaa cgccaagaag gccgcggtgg
     4741 ccgtcgggcc cgaggagcac gtgctgctgg ggcgggacca catcgtcgag tcgctcggcg
     4801 gcgtgcggtt cgaggtctcg gcgggctcgt tcttccagac caacacgctg caggccgagc
     4861 ggctcttcgc gctggtggcc gccgcctgcg agctcggcgg cggcgagacc gtcgtggacc
     4921 tctactccgg caccggcgcc atcagcctgc tcctggcgca gcgctgccgc caggtgtacg
     4981 gcatcgaggt cgcgccggcg gcggtagccg acgccgtgcg caacgcgcgg gagaacggca
     5041 tcgagaattg cacgttcctg gccggcgagg tgcgtcacgt gctgcccgcg ctggtgcgcg
     5101 acggcgtgcg cgccgacatc gtggtcgccg atccgccccg cgccgggttc catccgaaag
     5161 ccctcggcgc cctcgtcgcg ctcgcgccgg cgcgcctcgt ttacgtctcg tgcaatccgg
     5221 cgacgctcgc gcgcgacgtc ggagacctcc tgcgtcaggg ctatcgcctg gagtgggtgc
     5281 agccggtcga catgttcccg cagacgccgc atatcgaggc cgtcgcccgg ctccggcgcg
     5341 cgtaagcgcc cgcggcatga gagtctatct cgcccgccgg ctactccagt cgctcctggt
     5401 gctgctcggg gtctcgttcg tggtcttcgc gatcctgttc ctcaccggcg accccgcgct
     5461 ggtattgctg ccgcccgacg cgagcgccgc ggacgtccag gccttccgcg agcggatggg
     5521 gttcaacgac ccgttcttcg tccagtactg gcggttcctc cgcggcgcgc tccgcggcga
     5581 cttcggggag tcggtgcggc acggcgagcc ggcgttcccg ctggtcgtgg agcggctgcc
     5641 ggcgacgctc gagctctccg gcgcggcgct gctgatcgcg ctcgcgctgg cgatcccggc
     5701 cggcatcgtc tccgcggtgc ggcgcaactc gatggtggac tacgtctcca cggtgaccgc
     5761 gttgctgggc cagtcgatgc cgacgttctg gctggggatc atgctgatcc tggtcttctc
     5821 ggtgtggctc aacctgctgc cgtcttccgg ccgcggcggg ctggcgcacc tggcgatgcc
     5881 tgccatcacg ctcggtctct tcacgaccgc gcggatcatg cgcctcacgc gatcgggcat
     5941 gctcgaggtc ctcggccagg actacatccg cacagcgcgc gccaagggcg tcgccggggc
     6001 ccccgtcgtg tggaagcacg cgctgaagaa cgcggccatc cccatcgtga ccatcgtcgg
     6061 catcgagctc ggcacgctgc tcggcggtgc ggtcatcacc gagacgatct tcgcctggcc
     6121 gggcgtcggc cggctgagcg tgcaagcgat ctacaaccgg gactacccgg tggtgca
//
LOCUS       DATIAZ010000193         5681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1103503, whole genome shotgun sequence.
ACCESSION   DATIAZ010000193 DATIAZ010000000
VERSION     DATIAZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103503"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..240)
                     /locus_tag="VLF19_06860"
     CDS             complement(4..240)
                     /locus_tag="VLF19_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSE93009.1"
                     /translation="MSSPSDRPDDLWDAGDRGCGELLLELKMRVERLRPGQVLELVAR
                     DPGAPADLPAWCRLTGHALVEQRPPVYRIRRKES"
     gene            411..1514
                     /locus_tag="VLF19_06865"
     CDS             411..1514
                     /locus_tag="VLF19_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HSE93010.1"
                     /translation="MLRLLGLLAAAPLLVLTFSPFAQAQTEKPGSGPLRKATFDVRNE
                     ITVKVPDGAQRVRVWVALPQENDPAQVVRNLTIQAAYPYRIETDSEGSRVLYLEAAGP
                     KDRQFSIVETFTVTRSEVRDHIDASKARPITDADRQRFARYLRADKHVVIDDEIRKLA
                     DEIVGPETNPVLAARKLYDWVLKNVDYWVKDPKNKKASPVGSTTYCLTFRTGNCTDFE
                     SLWTSLARAKGIPTQIVYGSFLKPELRASDQDQSYHCWAEFYAPGLGWLHHDVAVADL
                     YEGDYPVNADNERLVRLTSADGTFGPDPARVGYYFGNLDERRVTFSRNRDLTLSPRQD
                     GEPVNALPKAYVEVDGKTHPEGAGWVRKLTYRE"
     gene            1511..2074
                     /locus_tag="VLF19_06870"
     CDS             1511..2074
                     /locus_tag="VLF19_06870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSE93011.1"
                     /translation="MISAITVISLLAVALTAGLVGLLAGLVTSAEAAALPDPPESLLV
                     QPVEASVADLGFKDLDGRDVRLEAFRGSVVLLNFWASWCVPCREEMPALERLQRAYSD
                     RGLVVVTVNFKETATKARAFIQEVQATGPVLLDPQGAAAGRLRVIGLPATFLLDRGAR
                     VIWKTLGERRWDSPPAREYFDRFVATP"
     gene            complement(2160..2996)
                     /locus_tag="VLF19_06875"
     CDS             complement(2160..2996)
                     /locus_tag="VLF19_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSE93012.1"
                     /translation="MDATPFRLVHLTDTHIIAEGPWRPRAGDFEFDTAASLRRVVEAV
                     RHLEPAPAFAVLGGDLASPDVFHRDRVLGPKDYEPSYVLLAEILAGLPCPIHVLMGNH
                     DQRVAFNRILRRGVTPDDAPCYYSFDHLGTHFVALDSHEPGQQGGVLDATQMAWLDDD
                     LARHRGRPTIVFVHHHPWPVGMEWMDGMSLKNGDDLMASLRRHGRVRWVICGHVHLDH
                     VVERDGLTMLTTPSTCIQLSKMSPQPRMLPGPPAFRVVDVAGDRLSTRVLHLHGASPR
                     DL"
     gene            complement(2999..4285)
                     /gene="murA"
                     /locus_tag="VLF19_06880"
     CDS             complement(2999..4285)
                     /gene="murA"
                     /locus_tag="VLF19_06880"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSE93013.1"
                     /translation="MDEFVIEGRAPLRGTVTPSGNKNAAFPLIAAALLTDEPVRLHNL
                     PDIADVRTILTLVERLGVDVDRHDAHTVTLRARRVTTTTPDADLMKQARGGLVLIGPL
                     LAREGEACVGHSGGDQIGRRRIDTHVLGLQALGARVEHDGQLVLRGRPLRGADILLDE
                     ASVTATENVVLAAALAEGTTVLSNAACEPHVQQTCQLLAAMGARIEGIGSNLLRIHGV
                     PRLGGAEFTLGPDFMEVGSFMALAAVTGGDITIKNAGLEHMRMALSVFGRLGVRTRAD
                     GDDLVLPGDQSLTIASDLGNAIPRVDDGPWPAFPADLMSVALVTATQAEGTVLFHEKM
                     YESRMFFVDRLIGMGARVVLCDPHRALIHGPSPLRGEPGGIPSPDIRAGIALVIAALC
                     AEGRTVIRSVEQIERGYENIEGKLQALGARIRRAKG"
     gene            4307..5074
                     /locus_tag="VLF19_06885"
     CDS             4307..5074
                     /locus_tag="VLF19_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE93014.1"
                     /translation="MVWDPAQYLKFAGQRLRPAVDLLARIDAADPAEVVDLGAGAGNV
                     TRLLKERWPKARITGVDDSLEMLAKAAAAAPEIAWNCADLATWRPARPVDVIFSNAAL
                     HWVSGHDRLFPALLAGLAPGGVLAVQLPRNFSAPSHTCISEAARSGPWRAKLEPLLRP
                     SPVAEPAFYFDLLVPRAATLDMWETEYLQVLDGEHAVREWVKGTWLRPLLDALEEPER
                     SRFEARYTELVAPYYPRRPDGRTLFPFRRLFIVATAR"
     gene            complement(5185..5457)
                     /locus_tag="VLF19_06890"
     CDS             complement(5185..5457)
                     /locus_tag="VLF19_06890"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HSE93015.1"
                     /translation="MIASPQPAERASQSPDAIGTSSALRELASKLKVEALVWAANPRE
                     RMVFMNGRKYVEGQALDDGVVVEEIAENGVVLVQGGQRVRVRAETR"
BASE COUNT          810 a         1994 c         2029 g          848 t
ORIGIN      
        1 gactcagctc tcctttcggc ggatccggta gacagggggt ctctgctcca ccagcgcgtg
       61 cccggtgagc cggcaccacg ccgggaggtc ggccggggcc ccggggtcgc gcgcgaccag
      121 ctccagcacc tgtccaggcc gaagccgctc gacacgcatt ttcagctcga gcagcagctc
      181 gccgcaaccg cgatcgcccg cgtcccacag atcgtcgggc cggtcgctcg gcgagctcat
      241 gctccccgaa tccccgctcg cacgaaacgc atcctacgcg ccatcaccat catgtgtcgt
      301 tggctcagtt gatgtctgac acccgccatc cgattcaagg tgccggctcc agcgtgtggc
      361 tgatcgcgac gttcgcctat cgaaccatcg cttcacatag gaggacttcg atgcttcgac
      421 tcctgggatt gctcgctgcg gctccgctcc tggttctcac gttcagtcct ttcgcgcaag
      481 cccaaacgga gaagccgggc tccgggccgc tgcgcaaggc aacgttcgac gtgcgcaacg
      541 agatcaccgt caaggtaccc gatggcgccc agcgcgtccg ggtctgggtc gcgctgccgc
      601 aggagaatga ccccgcccag gtcgtccgga acctgacgat ccaggctgca tatccgtatc
      661 gcatcgagac ggactccgag ggcagccgcg tgctctatct cgaggcggcc ggccccaagg
      721 acaggcagtt ctcgatcgtc gagacgttca cggtcacgcg gtcggaggtg cgcgaccaca
      781 tcgacgcgtc caaggcgcgc ccgatcaccg atgccgatcg ccagcggttc gcccgatacc
      841 tcagggccga caagcatgtg gtgatcgacg acgagatcag gaagctcgcg gacgagatcg
      901 tcgggcccga gacgaatccg gtactcgccg cgcgcaagct ctacgactgg gtgctcaaga
      961 acgtggacta ctgggtgaag gaccccaaga acaagaaggc gtcgcccgtc ggcagcacca
     1021 cgtactgcct gaccttccgc accgggaact gcaccgactt cgagtcgctc tggacttcgc
     1081 tggcgcgcgc gaaggggatt cccacgcaga tcgtctacgg gtcgttcctc aagcccgagc
     1141 tgcgcgcgag cgaccaggac cagagctatc actgctgggc ggagttctat gcgccggggc
     1201 tcggctggct ccaccacgac gtcgccgtgg ccgacctcta cgaaggcgac tacccggtaa
     1261 acgccgacaa cgagcgcctc gtgcgcctca cctcggccga cggcaccttc ggcccggatc
     1321 cggcccgcgt ggggtattac ttcgggaacc tcgacgagcg gcgcgtcacc ttctcgcgca
     1381 accgcgatct caccctgagc ccgcggcagg acggcgagcc cgtcaacgcg ctgcccaagg
     1441 cctacgtcga agtggacggc aagacgcacc ccgagggcgc cggatgggtt cgcaagctca
     1501 cctaccgcga gtgatctcgg cgatcacggt gatctcgcta ctcgccgtgg cgctgacggc
     1561 cgggctcgtc ggtctgctcg ccggtctggt gacctcggcc gaggctgcgg cgctccccga
     1621 tccgccggag tcgctgctcg tccagccggt ggaagcgagt gtcgccgacc tcggcttcaa
     1681 ggacctcgac gggagggacg tgcgcctcga ggcattccgt ggcagcgtcg tgctcctgaa
     1741 cttctgggcg agctggtgcg tgccgtgccg cgaggagatg ccggccctcg agcggctcca
     1801 gcgcgcctac agcgaccggg gactcgtcgt cgtcacggtg aacttcaagg agacggcgac
     1861 gaaggcacgc gccttcatcc aggaagtcca ggcgaccggt cccgtgctgc tcgatcccca
     1921 gggcgcggcc gccggtcgcc tccgggtgat cggattgccg gcgacgttcc tcctcgaccg
     1981 cggggcccgc gtgatctgga agacgctggg cgagcgccgc tgggattcgc cgccggcccg
     2041 cgagtacttc gatcgcttcg tggcgacacc ttaggaggtt gccgagccca tctgcttcga
     2101 ggtccccgcg cgcgcggtcg ggcgccgcct cgccactgga cattgcagcg gcgagccctt
     2161 cagaggtcgc gggggctcgc gccgtggaga tgaagcacgc gcgtcgacag ccgatcgccg
     2221 gccacgtcca cgacgcggaa ggccggcggg cccggcagca tcctcggctg cggggacatc
     2281 ttggagagct ggatgcacgt cgacggcgtc gtgagcatcg tgagcccgtc acgctcgacg
     2341 acatggtcga ggtggacgtg gccgcagatg acccagcgca cgcggccgtg ccgccggagg
     2401 ctcgccatca gatcgtcgcc gttcttcagc gacatgccgt ccatccactc catccccacc
     2461 ggccacgggt ggtggtggac gaagacgatg gtgggccgcc cgcgatgtcg cgcgaggtcg
     2521 tcgtcgagcc aggccatctg cgtcgcgtcg aggacgccgc cctgctggcc gggctcgtgg
     2581 gagtcgaggg cgacgaaatg ggtgccgagg tggtcgaagc tgtagtagca gggcgcgtcg
     2641 tcgggcgtca ccccgcgccg gaggatgcgg ttgaacgcca cgcgctggtc gtggttgccc
     2701 atgaggacgt ggatcgggca cggcaggcca gcgaggatct cggcgagcag gacgtacgag
     2761 ggctcgtagt ccttcgggcc cagcacgcgg tcgcggtgaa agacatcggg gctggcgagg
     2821 tcgccgccga ggacggcgaa ggccggcgcg ggctcgagat ggcgcacggc ctcgacgacg
     2881 cggcggagcg aggcggcggt gtcgaactcg aagtcgccgg cgcgcgggcg ccacgggccc
     2941 tcggcgatga tgtgggtgtc ggtcaggtgg acgaggcgga acggcgtcgc gtccaccgct
     3001 accccttcgc ccggcggatg cgggcgccca gggcctgcag cttgccctcg atgttctcgt
     3061 agccgcgctc gatctgctcc acgctgcgga tcaccgtgcg cccctcggcg cagagcgcgg
     3121 cgatgacgag cgcgatgccg gcgcggatgt cgggactggg gatgccgccc ggctcgccgc
     3181 gcagcgggct cggcccgtgg atcagcgccc ggtgggggtc gcagagcacc acgcgcgcgc
     3241 ccatgccgat gagccggtcg acgaagaaca tccggctctc gtacatcttc tcgtggaaga
     3301 ggacggtgcc ctcggcctgc gtcgccgtga ccagcgccac gctcatgagg tcggccggga
     3361 acgccggcca cggcccgtcg tcgacccgcg ggatggcgtt gccgaggtcc gaggcgatcg
     3421 tgagcgactg gtccccgggc agcaccaggt cgtcgccgtc cgcgcgggtc cgcacgccca
     3481 gccgcccgaa caccgagagc gccatgcgca tgtgctcgag gccggcgttc ttgatcgtga
     3541 tgtcgccgcc cgtcaccgcg gccagggcca tgaagctgcc gacttccatg aagtcgggac
     3601 cgagcgtgaa ctccgcgccg ccgagacgcg gcacgccgtg gatccgcagc agattcgagc
     3661 cgatgccctc gatgcgagcg cccatggccg ccaggagctg acacgtctgc tgcacgtgcg
     3721 gctcgcaggc ggcgttgctc agcaccgtgg tgccctcggc gagcgcggcg gccagcacga
     3781 cgttctccgt cgcggtcacg ctggcctcgt cgagcaggat gtcggcgccc cggagcggac
     3841 ggccgcgcag cacgagctgg ccgtcatgct cgacgcgcgc gccgagggcc tgcaatccga
     3901 gcacgtgggt gtcgatgcgc cggcgcccga tctggtcgcc gccggagtgg ccgacgcagg
     3961 cctcgccctc gcgcgcgagc agggggccga tgaggacgag gccgccgcgc gcctgcttca
     4021 tgagatcggc gtccggcgtc gtcgtggtga cgcgccgggc gcggagcgtg acggtgtggg
     4081 cgtcgtggcg gtccacgtcc acgccgaggc gctcgacgag cgtgagtatg gtgcggacgt
     4141 cggcgatgtc cggcaggttg tgcaggcgca cgggctcgtc ggtgaggagc gccgcggcga
     4201 tcagggggaa ggccgcattc ttgttgccgc tgggggtgac ggtgccccgc aggggggcgc
     4261 ggccttcgat gacgaattcg tccatgccct aggatatcct gccgccatgg tctgggaccc
     4321 cgcgcaatat ctcaagttcg ccgggcagcg cctgcggccg gcggtggacc tcctggcccg
     4381 gatcgacgcc gccgaccccg ccgaggtcgt ggacctcggc gccggcgccg ggaacgtcac
     4441 ccgcctgctc aaggagcgct ggccaaaggc gcggatcacc ggcgtcgacg actccctgga
     4501 gatgctggcg aaggcggcgg cggccgcgcc ggagatcgcc tggaactgcg ccgacctcgc
     4561 gacgtggcgg cccgcccgcc cggtcgacgt catcttctcc aacgccgccc tgcactgggt
     4621 gagcggccac gaccgcctat tcccggcgct cctcgccggg ctcgccccgg gaggcgtcct
     4681 cgccgtgcag cttccgcgca acttctcggc gccgtcccac acatgcatct ccgaggccgc
     4741 ccggagcggg ccgtggcgcg cgaagctcga gccgctgctc cggccgtcgc cggtggccga
     4801 gccggccttc tacttcgatc tcctcgtccc gcgggccgcg accctcgaca tgtgggagac
     4861 cgagtacctc caggtgctcg acggcgagca cgcggtcagg gagtgggtga agggcacgtg
     4921 gctccgtccg ctgctcgacg cgctggagga acccgagcgg tcacgcttcg aggcccgcta
     4981 caccgagctc gtcgcgccgt actatccgcg gcgaccggac ggccgcacgc tcttcccgtt
     5041 caggcgcctg ttcatcgtcg ccaccgctcg ttgacacgcc gggagccgcg gtccggctcg
     5101 ccgacccctt cggctaccgg tggacgctgt ccaccaaggt cgaggaggtc tccgaggagg
     5161 aggtcctcag gcgcgcgacg gcggtcacct cgtctccgct cgcacccgta cgcgctggcc
     5221 tccctggacg aggacgacgc cgttctcagc gatctcctcg acgacgacgc cgtcgtctag
     5281 ggcctggccc tcgacgtact tgcggccgtt catgaacacc atccgctctc ttggattggc
     5341 cgcccagacg agcgcctcga ccttgagctt cgacgccagc tcgcgcagag cactcgacgt
     5401 cccgatcgcg tccggagact ggctcgctcg ctcggcgggc tggggcgacg cgatcaacgg
     5461 cgcggtccgg gtcgggggcg tgggtgtcgt ggctcccggc gcggccgtgg tctgcgctgg
     5521 cgaggtcgaa cgcgggggtg acggacgctg cgcgggcgcg accggggcct ggggcgccgg
     5581 gcgcagcgcg ggagccgtag tctctgcgga gggcgtggcg gccgttggtg ccagccgttg
     5641 cgcaggcggc gtggcttctg cgggtaccgt ggcagcccgg g
//
LOCUS       DATIAZ010000194         4658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1234744, whole genome shotgun sequence.
ACCESSION   DATIAZ010000194 DATIAZ010000000
VERSION     DATIAZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234744"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..763
                     /locus_tag="VLF19_06895"
     CDS             425..763
                     /locus_tag="VLF19_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSE93016.1"
                     /translation="MKKIEAIIKPFKLDDLKEALTGIGVIGMTVSEVRGFGRQKGHTE
                     LYRGGEYTVDFLPKIKVEVVVPDHLAGKVADVIAGAAKTGNIGDGKIFICPVEAAIRI
                     RTGERDESAL"
     gene            828..2240
                     /locus_tag="VLF19_06900"
     CDS             828..2240
                     /locus_tag="VLF19_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSE93017.1"
                     /translation="MTLVLGGTAAVTFAQQPAAPAAPAAPAAAPEAPKIDKGDTAWLL
                     TSSALVLMMTAPGLALFYAGMVRQKNTLATLMHSFIIMAVISVQWVLWGYSLAFGPDK
                     GGIIGGLEWIGLSGVGGDPNPAYAATIPHSVFMLFQMMFAIITPALITGAFAERKKFS
                     AFLLFTVLWATFVYDPLAHWVWGDGGWLKKLGALDFAGGTVVHISSGVSALVCAIMIG
                     RRRGYGHQPMQPHNLTMTVMGAALLWFGWFGFNAGSALEANALAASAFLATNTAAAAA
                     ALGWMFTEWTTRGKPTVLGAASGAVAGLVAITPASGFVGPVASIFIGALAGGLCYTAC
                     NLKSKLGYDDSLDVVGVHAVGGTWGAIATGLFATKTVNGAGGDGLLYGNPGQLWIQIV
                     AVLATYALAVVGTVIILKVVDVIVGLRVTEEDEVAGLDLSQHSETAYVLGGGSYSEFS
                     GGGAFPEAMRSAEAKARTAH"
     gene            2368..3792
                     /gene="glnA"
                     /locus_tag="VLF19_06905"
     CDS             2368..3792
                     /gene="glnA"
                     /locus_tag="VLF19_06905"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSE93018.1"
                     /translation="MTAKDVLKLAKEKGAKIVDLRFIDLPGLWQHFSVPVSELSEDIF
                     EDGLGFDGSSIRGFQTIDESDMLLIPDASTAVMDPFTAVPTLVLVCNVKDPVTGKPYT
                     RDPRYIAQKAEAYLKKSGVGDTVYIGPELEFFFFDSIRFDQNYNSGYYFIDSEAGAWN
                     TGREGSADNPNLGYKPRYKQGYFPVPPMDKFQDIRSDMVLALESVGVRVEVHHHEVAT
                     AGQTEIDMRYDTLTKMADKVMWYKYCAKNTARKWGKTATFMPKPLFQDNGSGMHTHQS
                     IWKNGKNLFYERGGYADISKTCLYYIGGILKHAPALLALIAPSTNSYKRLVPGYEAPI
                     NLVYSQRNRSAAIRIPVYSKSEGAKRIEFRTPDPTCNPYLSFAACMMAGLDGIANKID
                     PGKPVDKDLYELPAAEQKKIKQLPGDLAIVLDNLEKSHDWLLKGDVFTPDVIETWLEY
                     KRKNEVDAVRLRPHPWEFALYFDI"
     gene            4248..>4658
                     /locus_tag="VLF19_06910"
     CDS             4248..>4658
                     /locus_tag="VLF19_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93019.1"
                     /translation="MLDDHERSRAELRVAIEAAGGEVADEAVRCTEVLEIVRAARPDV
                     VVCAVGLADGDGIDAAAQVVAEGCPVVLFTSHTGDELVERARAAGVMALLLKPLRPAE
                     LAPALDLAVARFTETQRLRRRLVERKVIDRAKGLL"
BASE COUNT          739 a         1598 c         1592 g          729 t
ORIGIN      
        1 ctcaaggcgc tcgagagcac gcgctggaac aagcaggccg ccgccggtct cctggggctg
       61 cgccggccca cgctctactc gaagatgcgc aagcacggga tcccgcagcg gccctgaacc
      121 tccccaggat tcgggttgca ccggcagagc ccgcgcgcga ccgacgcccg ccgtgcactc
      181 gggattctcg gagcgacgtc cgtcgccccg cggtgcacgc tgggccggcc gggcgcccag
      241 gggaaggcct agaacggcgg cttgaaacgc aggcagttca gcgagtgctg cctattcgct
      301 gagcatcgcc tggtcgcggg gccgtcgtcc catgctatcc aaatcattgg ttttccaggc
      361 ggtcctcggg tggaagggca ggcacagtca ttgctcggct gcacgtgcgc ccggggggga
      421 accgatgaaa aagatcgagg ccatcatcaa gccgttcaag ctcgacgacc tgaaggaggc
      481 gctcaccggc atcggcgtca tcggcatgac cgtgtcggag gtgcgcgggt tcggccgcca
      541 gaagggccac accgagctgt accgtggcgg tgagtacacc gtcgacttcc tgccgaagat
      601 caaggtcgag gtcgtggtgc ccgatcatct cgccggcaag gtggccgacg tcatcgccgg
      661 cgccgccaag accgggaaca tcggcgacgg caagatcttc atctgtcccg tggaggccgc
      721 catccggatc cgcacgggcg agcgcgacga gagcgccctg tgaggcccac ggggacctcg
      781 gggccacaag gaggctcacg gtgaagcgac tgactctggc acttgcaatg acgctcgtgc
      841 tcggcggcac tgcggccgtc accttcgcac agcagccggc cgcgcccgcc gcacccgcgg
      901 cgccggcggc ggcgcccgag gcgccgaaga tcgacaaggg cgacacggcc tggctgctca
      961 cctcgtcggc cctcgtcctg atgatgacgg cgcccgggct cgccctcttc tacgcgggca
     1021 tggtccggca gaagaacacg ctcgccacgc tcatgcacag cttcatcatc atggccgtga
     1081 tcagcgtgca gtgggtcctc tggggctaca gcctcgcctt cggccctgac aagggcggca
     1141 tcatcggcgg gctcgagtgg atcgggttga gcggcgtggg cggcgatccc aatcccgcgt
     1201 acgcggcgac catcccgcac tcggtgttca tgctgttcca gatgatgttc gccatcatca
     1261 cgcccgcgct catcaccggc gccttcgccg agcgcaagaa gttctcggcg ttcctgctgt
     1321 tcacggtgct gtgggcgacg ttcgtctacg acccgctggc gcactgggtg tggggcgacg
     1381 gcgggtggct caagaagctg ggcgcgctcg acttcgccgg gggcaccgtc gtgcacatct
     1441 cgtccggcgt ctcggcgctg gtctgcgcga tcatgatcgg ccggcggcgg ggctatggcc
     1501 accagccgat gcagccgcac aacctgacga tgaccgtgat gggcgcggcg ctgctctggt
     1561 tcggctggtt cggcttcaac gccggcagcg ccctcgaggc caacgcgctg gcggcctcgg
     1621 ccttcctggc gaccaacacg gcggcggccg cggcggcgct gggctggatg ttcaccgaat
     1681 ggacgacgcg cggcaagccg acggtcctgg gcgcggcctc gggcgccgtc gccgggctcg
     1741 tggccatcac gccggcctcg ggcttcgtgg gcccggtcgc gtccatcttt atcggcgcgc
     1801 tcgccggcgg cctctgctac acggcctgca acctcaagtc gaagctcggc tacgacgact
     1861 cgctcgacgt ggtcggcgtg cacgcggtgg gcggcacgtg gggcgccatc gcgaccggcc
     1921 tgttcgccac caagacggtc aacggcgccg gcggcgacgg cctcttgtac ggcaaccccg
     1981 gccagctctg gatccagatc gtggccgtgc tcgccaccta cgcgctggcg gtcgtcggca
     2041 ccgtgatcat cctgaaggtc gtggacgtca tcgtcggcct gcgggtgacc gaggaggacg
     2101 aggtggccgg tctggacctc tcgcagcact cggagacggc ctacgtcctc ggcggcggct
     2161 cgtacagcga gttctcgggc ggcggcgcct tcccggaggc catgcgaagc gcggaagcca
     2221 aggcccggac cgctcactga cgcatgcgcg ccttgatttc acgggcgtag ttccgtaagg
     2281 tagccgggcc cggcggcgcc tccgcgcgtc gccgggtcgc tcggccgaca ctcaacgacc
     2341 tcgctggagg ggagaaggag ttctctgatg accgcgaagg acgtcctcaa gctggccaag
     2401 gagaaaggcg ccaagatcgt cgatctccgc ttcatcgacc tgcccgggct ctggcagcac
     2461 ttctcggtcc cggtgtccga gctcagcgag gacatcttcg aagacggact gggattcgac
     2521 ggctcgtcga tccgaggctt ccagacgatc gacgagtccg acatgctcct gatcccggac
     2581 gcctcgacgg ccgtgatgga ccccttcacg gccgtgccca cgctcgtcct cgtctgcaac
     2641 gtcaaggacc cggtgaccgg caagccatac acgagggacc cgcgctacat cgctcagaag
     2701 gccgaggcgt acctcaagaa gagcggcgtg ggggacacgg tctacatcgg gccggagctc
     2761 gagttcttct tcttcgactc gatccgcttc gaccagaact acaactcggg ctactacttc
     2821 atcgactccg aggcgggcgc ctggaacacc ggccgggagg gttccgcgga caatcccaac
     2881 ctcggctaca agccgcgcta caagcagggc tacttcccgg tcccgccgat ggacaagttc
     2941 caggacatcc gctcggacat ggtgctcgcg ctggagagcg tcggggtccg cgtggaggtc
     3001 catcaccacg aggtggccac cgccgggcag accgagatcg acatgcggta cgacacgctg
     3061 acgaagatgg ccgacaaggt catgtggtac aagtactgcg ccaagaacac cgcgcggaag
     3121 tggggcaaga cggccacctt catgccgaag ccgctcttcc aggacaacgg ctccggcatg
     3181 cacacgcacc agtcgatctg gaagaacggc aagaacctct tctacgagcg gggcgggtac
     3241 gccgacatct ccaagacctg cctgtactac atcggcggca tcctcaagca cgcgcctgcg
     3301 ctgctggcgc tcatcgcgcc gtcgaccaac tcgtacaagc ggctggtgcc gggttacgag
     3361 gcaccgatca acctcgtcta cagccagcgc aaccgctcgg cggccatccg gatcccggtg
     3421 tactccaagt cggagggcgc caagcgcatc gagttccgca cgccggaccc gacctgcaac
     3481 ccgtacctct cgtttgccgc gtgcatgatg gcgggcctcg acggcatcgc caacaagatc
     3541 gacccgggca agccggtgga caaggatctc tacgagctgc cggcggccga gcagaagaag
     3601 atcaagcagc tgccgggcga cctcgcgatc gtgctcgaca acctcgagaa gtcgcacgac
     3661 tggctgctca agggcgacgt cttcacgccg gacgtcatcg agacgtggct cgagtacaag
     3721 cgcaagaacg aggtcgacgc ggtgcggctc cgcccgcatc cgtgggagtt cgcgctgtac
     3781 ttcgatattt gacgaccgac cacgatgggg ggcccgaacg ggccccccca aggcccgcgt
     3841 tcggagcgct ccggggaagc cggggcggtc ctctcgagcg cacgacccgc gagttcttcc
     3901 ctcgacgcag acccgtgacg tgcttccttc gatctcgccc tgcccgcggt tctcttcttc
     3961 ggcgtcgggg ggcccgcgat ctcgctgctt cgacgtcgcg ctgtccgtga tctccttgcg
     4021 cgatgtcgcc gcgcttcgtg agtccgcgcc gctgcgggag cttcacggga tgcccgtcgc
     4081 ccggcgggga tttgcctggc aaaggagcat cgtggcgcca ctgccgacgg ggcaggcagt
     4141 ggcgtccgga gcgtctcgcg caagttcccg attccggtga cggagatgtg gcatcgccct
     4201 tgccgccacg ccggggcgtg aagcaagcgg cacggtggcg tgtcatcgtg ctcgacgacc
     4261 acgagcgctc gcgcgcggag ctgcgcgtgg cgatcgaggc ggcgggcggg gaggtggcgg
     4321 acgaggcggt ccgctgcacc gaggtcctcg agatcgtccg cgctgcgcgg cccgacgtgg
     4381 tggtctgcgc ggtcggcctg gcggacggtg acggcatcga tgccgcggcc caggtcgtgg
     4441 ccgagggctg cccggtcgtg ctcttcacga gccacaccgg tgacgagctc gtcgagcgtg
     4501 cccgcgccgc cggcgtgatg gcgctcctgc tcaagccgct gcgtccagcc gagctggccc
     4561 cggcgctcga cctggccgtg gcgcgcttca ccgagacgca gcgcctgcgc cggcgcctgg
     4621 tggagcgcaa ggtcatcgat cgcgcgaagg gcttgctg
//
LOCUS       DATIAZ010000195         8036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_52054, whole genome shotgun sequence.
ACCESSION   DATIAZ010000195 DATIAZ010000000
VERSION     DATIAZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52054"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VLF19_06915"
     CDS             <1..224
                     /locus_tag="VLF19_06915"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HSE93020.1"
                     /translation="GALDTLEERVEERPEVAGAGDGALRDAALHVRRRVHEVIGLTVD
                     VTALRPRTLERAPGKACRVQDLRAQGGGA"
     gene            221..382
                     /locus_tag="VLF19_06920"
     CDS             221..382
                     /locus_tag="VLF19_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93021.1"
                     /translation="MSRSRVWGSNGLPWNESLDQLDAPGLRPGARQKPVRDNDCALFS
                     LELSAGGVR"
     gene            379..1176
                     /locus_tag="VLF19_06925"
     CDS             379..1176
                     /locus_tag="VLF19_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE93022.1"
                     /translation="MNPGLVLGAIVLVAVLFVLLPVGGAAYSRFRRRWRLRCPAEGVE
                     ADVRIDPGEAARAEIFGRSGPTVAHCSLWPERRGCAAECLHSPAHEWRHPGPGERPPR
                     PAGADDRSTILVPLDGTPGSERALNEVVDLARARRARLRLLRVARLMPELRVDSRVIA
                     FADQETARVELETLAYLRQLAEGLGDIETDPAVRFGDPEAEIVKDAEVARADLIAMAT
                     RRRHGLAGMLQESVAQKVQRAADVPVLLVPYGAYEPGHTLAGGTGSA"
     gene            1208..2899
                     /locus_tag="VLF19_06930"
     CDS             1208..2899
                     /locus_tag="VLF19_06930"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSE93023.1"
                     /translation="MASPVRRAIGWATIVVLLGCSGSPASRPIAPVRVQHAGATASAQ
                     PPTPPAAPGEPVPVRLTPSTTRAATPELLAVGKQVYDKQCAACHGPDGRGEGEAAYLL
                     YPKPRDFTGGAYRLVSTWERVPTDEDLFRTISRGMPGSSMPSWGHLSEEERWGLVHYV
                     KTFARTPIAVRPASDPRGEGQSGTGVITVPPPAPFTPEARQLALERFADACASCHGSR
                     GKGDGTEEQKDDLGYPTRPRDLTVGVFKGDPDPVQLYRRIVAGLPGTPMPMSDWAYGA
                     DAWHLVNLILSWSSPEQRARAEMRRFRIVARRVDRVPDHPDAGAWRVAQPVNLHLMPL
                     WWRSDRPEEITVRALHDGRQLALLLVWADATHDHTAMRPQDFRDAVAVQLSPTPEPPF
                     FAMGSTGQFVNIWMWKSERQADLEPAFQELEKVYPNLGIDSYPDPSVSPVEQPTRHAL
                     TLGSDRDFITGWGAGNIVSDPSRASPAEDLVAQGFGTLRARPRPDQAVHARGVYATGA
                     YRVMLRRDVVGRGERAVTLTPGTTLSVGFAVWNGSAGDRDGKKSVTIWQDLVLAP"
     gene            2914..6348
                     /locus_tag="VLF19_06935"
     CDS             2914..6348
                     /locus_tag="VLF19_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE93024.1"
                     /translation="MDRRDFLRWLGIGTGTTVAELGLPLNLLAAAGPDQNPLAGSVTR
                     DWERIYRDQYRYDSTFDWVCSPNDTHACRVRAFVRNGVVTRLGNTYDYQTYADLYGNR
                     ATANWNPRQCAKGYTFHRVLYGPYRLKHPIVRKGWKAWVDAGFPELTPELRSKYMFDA
                     RGQDEFVQIAWEDAFACIAKALVAIAERYSGEPGKERLLAQGYQPEMVGATGGAGTRC
                     IKMRGGMGLLGVIGKYGMYRLNNSLALLDARIRRVTPQDARGGRNWSNYTWHGDQAPG
                     QPWVHGLQASDCDFNDLRFSKLIIMDGKNLVENKLTDSHWFIECMERGAKIVVIAPEY
                     GPPSTKADYWIPIRPQTDAALWLGVTRLMIEQRWYDEAFVKAFTDFPLLVRTDTLKRL
                     RAHEVFPGYRTTLSSDGPSARVQGLTPDQHAKLGDYVVWDDKSGGLRALTRDDVGDRM
                     RAAGVDPALDRGLTVKLVDGREVEVATLWRLYRKHLEDYDLDTVAEITHSPKALIQQL
                     AQDIGATKPVSIHQGEGINHWFHATEMNRAAYLPLMLTGNIGRPGAGCHTWAGNYKAA
                     LFQGSPRTGPGFKGWVAEDPFHLNLDASAHGRDVEAHAYTKDEEPAYWNHGDQALIVN
                     TPKFGRRIFTGDSHMPTPTKAIIFSNVNLINNAKWAYGVIRNVNPNVEMICAIDIQMT
                     ASIEYADIALPANSWLEFEGLEITASCSNPFLQIWKGGIPPVFDSRDDLMILAGIAEA
                     LAGVTGDRRFADCFKFALEGRREVYIQRLLDTCTTTAGYALADIMAGKYGPPGGALML
                     FRTYPRIPFWEQVHDSEPFHTDTGRLHAYADVPEAIEYGENFVVHREGPEATPYLPNV
                     IVSSNPFIRPESYGIAETAEHWDERTIRNVKLPWSRVKETQGFLWKQGYRFYCLTPKT
                     RHRVHSSWSNVDWHMLFDSNFGDPYRLDRRAPSVGEHQLHMNPQAARDLGIADGDYVY
                     VDANPADRPYAGAKPGDPFYRVARCMLRVKYNHAYPYHCVMMKHAPFIATERSVKAHE
                     TRPDGRALSENTGYQANLRYGSQQSVTRNWHMPMHQTDTLFHKAKAFMGFIFGGEADN
                     HALNTVPKETLVKITKAEDGGLGGRGVWAPATTGFTPDNESDFMKRYLAGDFYRKA"
     gene            6351..6662
                     /locus_tag="VLF19_06940"
     CDS             6351..6662
                     /locus_tag="VLF19_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93025.1"
                     /translation="MPYGEPDPGDPSVLVGVTLPAGAEAVRDMAWVFAEEFARLGYDA
                     RRILGLFRSPFYAGAHHALRALGETEVAAIIEECVSAWRRPPRGDATPPGGGGRPPQG
                     D"
     gene            6666..7931
                     /locus_tag="VLF19_06945"
     CDS             6666..7931
                     /locus_tag="VLF19_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSE93026.1"
                     /translation="MATVHNWQLGREMSYPYESAYPERQFAFVFNINRCIACQSCTMA
                     CKSTWTFSKGQEHMWWANVETKPYGGYPMFWDAKILELLERANPEGTRWTGALADDPR
                     QPYGRFDGRTIFEATRMLTPDSARVLGYLPTDAEWNSPNIYEDNPVGQRGTPGEYHRT
                     GESLPEHKTWFFYLARICNHCSYPACLAACPRKAIYKRPEDGIVLIDQAECRGYRKCV
                     EACPYKKSMYRGNTRVSEKCVACYPRVEGKDPENPGIPLETRCMAACIGQIRLQGLVK
                     LNRDGTWAEDRHNPIYYLVRVAKVALPLYPQLGTEPNGYYIPPRWVPRPYLRQMFGPG
                     VDQAIERYENPDRELTAVLQLFRRSNRIIYGYKVEDGPKVHEATLRGRKVTLYNDTVI
                     AYGKDGNEIFRTTVEEPVHVRPPRHANSI"
BASE COUNT         1299 a         2871 c         2746 g         1120 t
ORIGIN      
        1 agggtgcgct cgacaccctg gaggagcggg tcgaggagag gccggaggtg gccggcgcgg
       61 gcgacggcgc gctgcgcgac gccgcgctcc acgtccggcg gcgcgtccac gaggtgatcg
      121 gcctcacggt ggacgtcacc gcgctgcggc cgcggacgct cgagcgcgcc ccgggcaagg
      181 cgtgccgggt ccaggacctc cgggcccagg gaggtggagc atgagcaggt cgcgcgtgtg
      241 gggcagcaac ggcctgccgt ggaatgagag cctggaccag ctcgacgcac cgggcctgcg
      301 gcccggagcc cggcagaagc cggtccgcga caacgactgc gcgctgttca gtctcgagct
      361 gtcggcagga ggtgtgcgat gaatccgggg ctggtgctcg gagcgatcgt cctcgttgcg
      421 gtgctcttcg tgctgctccc cgtcggcgga gcggcctact cgcggttccg gcgtcgctgg
      481 cgtctccggt gccccgcgga gggcgtcgag gctgacgtca gaatcgatcc cggcgaggcc
      541 gcccgggccg agatcttcgg gcgctccggc cccacggtgg cccactgctc gctgtggccc
      601 gagcgccgcg gatgcgcggc ggagtgcctg cactcgcccg cgcacgagtg gcggcacccg
      661 ggcccgggag agcggccgcc gcggccggcg ggcgccgacg accgttccac gatcctggtc
      721 ccgctggacg gcacccccgg cagcgagcgt gcgctgaacg aggtcgtcga cctggctcgc
      781 gcccgccggg cacgtctgcg cttgctccgg gtggcgcggc tcatgccgga gctccgcgtc
      841 gactcccggg tcatcgcctt cgccgatcaa gaaactgcgc gcgtggagct cgaaaccctg
      901 gcgtacctcc ggcagctggc cgagggcctg ggcgacatcg agaccgaccc cgccgtgcgc
      961 ttcggcgacc ccgaggccga gatcgtgaag gacgccgagg ttgcccgggc cgacctcatc
     1021 gcgatggcga cgcgccgccg gcacggactc gccggcatgc tccaggaaag cgtggcccag
     1081 aaggtccagc gcgccgccga cgttccggtg cttctcgtcc cttatggtgc ttatgagccc
     1141 ggtcataccc tggcgggcgg gacgggttca gcatgaatgc acgacaacga gggggagaag
     1201 gctgccgatg gcgtctccgg tgcggcgagc gatcgggtgg gcgacgatcg tggtcctgct
     1261 gggctgttcg ggatcccccg cctcacgccc gatcgctccc gttcgggtgc agcacgcggg
     1321 ggccacggca tccgctcagc cgccgacgcc cccggcggcg cccggtgagc cggtccccgt
     1381 caggctgacg ccctcgacga cgcgcgctgc cacgcccgag ctcctggcgg tcggcaagca
     1441 ggtctacgac aagcagtgcg ccgcctgtca cggccccgac gggcgcggcg agggcgaggc
     1501 ggcctacctt ctctatccga agcctcgcga cttcacgggg ggcgcgtacc gcctcgtctc
     1561 cacgtgggag cgcgtcccga cggacgagga cctcttccgg acgatctcgc ggggcatgcc
     1621 gggctcgtcg atgccctcct gggggcatct ctccgaggag gagcggtggg ggctggtcca
     1681 ctacgtcaag accttcgccc ggacaccgat cgccgtccga cccgcctccg atccacgagg
     1741 cgaaggccag agcggcaccg gcgtgatcac ggtgccgccc cccgcgccgt tcaccccaga
     1801 agcgcggcag ctcgcgctcg aacggttcgc ggacgcctgt gcgtcgtgcc acggcagccg
     1861 cggcaagggt gacggcaccg aggagcagaa ggacgacctc ggctatccca cgcgcccgcg
     1921 cgatctcacg gtcggcgtgt tcaagggcga ccctgatccg gtccagctct accggaggat
     1981 cgtggcgggc ctgcccggta ccccgatgcc gatgagcgac tgggcgtacg gagccgacgc
     2041 ctggcatctg gtgaacctga tcctctcgtg gtcgagcccc gagcagcgcg cgcgggcgga
     2101 gatgcgccgg ttccggatcg tcgcgcgccg ggtcgaccgc gtgccggatc atccggatgc
     2161 cggcgcctgg agagtcgccc agccggtgaa cctgcacctc atgccgctct ggtggcggag
     2221 cgaccggccg gaggagatca cggtgcgcgc gttgcacgac ggccggcagc tcgcgctcct
     2281 gctcgtgtgg gcggacgcga cgcacgatca caccgcgatg cggccgcaag acttccgcga
     2341 cgccgtcgcg gtccagctct ccccgacccc ggagccgccg ttcttcgcca tgggcagcac
     2401 ggggcagttc gtgaacatct ggatgtggaa gtcggagcgc caggccgacc tggaacccgc
     2461 cttccaggag ctggagaagg tctatcccaa cctgggcatc gactcttatc cggatcccag
     2521 cgtctcgccc gtcgagcagc cgacccggca cgcgctcacg ctcggatcgg atcgcgattt
     2581 catcacgggc tggggggccg ggaacatcgt gtcggacccg agccgggcga gccctgccga
     2641 agatctcgtc gcccagggct tcgggacgct gcgcgcccgg ccgcggccgg accaggccgt
     2701 ccacgcgcgg ggcgtgtacg cgaccggcgc ctaccgcgtc atgcttcgcc gcgacgtcgt
     2761 cggccgtggc gagcgcgccg tcacgttgac gcccgggacg acgctgtccg tcggcttcgc
     2821 cgtctggaac ggcagcgccg gcgaccggga cggaaagaag tccgtcacga tctggcagga
     2881 cctcgtgctc gcgccgtagt cgggaggact tccatggacc gtcgcgattt tctccgctgg
     2941 ctcggcatcg gcaccgggac gaccgtcgcc gagctggggc tgccgctcaa cttgctggcc
     3001 gccgccggtc ccgaccagaa cccgctggcc ggctccgtga cccgcgactg ggaacggatc
     3061 taccgcgacc agtaccggta cgactcgacc ttcgactggg tctgctcgcc gaacgacacc
     3121 cacgcctgcc gcgtgcgggc cttcgtgcgc aacggcgtcg tcacccggct cggcaacacg
     3181 tacgactacc agacgtacgc cgacctctac ggcaaccgcg ccacggccaa ctggaacccg
     3241 cgccagtgcg ccaagggcta caccttccat cgcgtgctct acggacccta ccggctcaag
     3301 cacccgatcg tgcgcaaggg atggaaggcg tgggtcgacg ccggattccc cgagctgacg
     3361 cccgagctgc ggtcgaagta catgttcgac gcccgcggcc aggacgagtt cgtgcagatc
     3421 gcctgggagg acgccttcgc ctgcatcgcg aaggcgctgg tggcgatcgc cgagcgctac
     3481 agcggcgagc cgggcaagga acggctcctg gcccagggtt accagccgga gatggtcggc
     3541 gccacgggcg gggccggcac gcgctgcatc aagatgcgcg gcggcatggg gctcctcggc
     3601 gtgatcggca agtacgggat gtaccgcctg aacaactcgc tggccctcct cgacgcccgg
     3661 atccgccgcg tgaccccgca ggacgccagg ggcgggcgga actggtcgaa ctacacctgg
     3721 cacggcgacc aggcgcccgg ccagccgtgg gtgcacgggc tgcaggcctc cgactgcgac
     3781 ttcaacgacc tgcgcttctc gaagctgatc atcatggacg gcaagaacct ggtggagaac
     3841 aagctgaccg attcccactg gttcatcgag tgcatggagc ggggcgcgaa gatcgtcgtc
     3901 atcgctcccg agtacggccc gccgtccacc aaggccgact actggatccc gatccggccc
     3961 cagaccgacg ccgctctctg gctcggcgtg acccgcctga tgatcgagca gcggtggtac
     4021 gacgaggcct tcgtcaaagc gttcaccgac tttccgctcc tcgtccgcac ggacacgctc
     4081 aagcgcctgc gcgcgcacga ggtgttcccc ggctaccgga cgacgctctc gtccgacggc
     4141 ccgtcggcga gggtccaggg gctcacgccg gaccagcacg cgaagctcgg cgactacgtc
     4201 gtctgggacg acaagagcgg cggcctgcgc gcgctgacgc gggacgacgt cggggaccgg
     4261 atgcgcgcgg cgggcgtgga cccggcgctc gaccgggggc tcacggtgaa gctggtggac
     4321 ggccgcgagg tcgaggtggc caccctctgg cgactctacc ggaagcacct cgaggactac
     4381 gacctcgaca ccgtcgccga gatcacccat tcgccgaagg ccctgatcca gcagctggcc
     4441 caggacatcg gcgccacgaa gccggtctcg atccaccagg gcgagggcat caatcactgg
     4501 ttccacgcga cggagatgaa ccgcgccgcc tacttgccgc tgatgctgac cgggaacatc
     4561 ggccggccgg gggcgggctg ccacacgtgg gccggcaact acaaggcggc gctgttccag
     4621 ggctcgcctc gcacgggccc cggcttcaag ggctgggtcg ccgaggatcc cttccacctg
     4681 aacctcgacg cctccgccca cggccgcgac gtcgaggcgc acgcgtacac gaaggacgag
     4741 gagccggcgt actggaacca cggcgaccag gcgctcatcg tgaacacgcc gaagttcggc
     4801 cggcggatct tcaccggcga cagccacatg cccacgccga cgaaggcgat catcttcagc
     4861 aacgtcaacc tgatcaacaa cgccaagtgg gcgtacggcg tcatcaggaa cgtcaacccc
     4921 aacgtggaga tgatctgcgc gatcgacatc cagatgaccg cgtcgatcga gtacgccgac
     4981 atcgccctgc ccgccaacag ctggctcgag ttcgaggggc tggagatcac cgcgagctgc
     5041 tccaatccgt tcctgcagat ctggaagggc ggcatcccgc cggtgttcga cagccgcgac
     5101 gacctgatga tcctggccgg catcgccgag gcactcgccg gcgtcaccgg cgaccggcgc
     5161 ttcgccgact gcttcaagtt cgccctggag ggccgtcgcg aggtctacat ccagcggctc
     5221 ctcgacacct gcacgaccac cgccggctac gcgctcgccg acatcatggc cggcaagtac
     5281 gggcctccgg gcggcgcgct gatgctgttc cggacttacc cgcgcatccc gttctgggag
     5341 caggttcacg acagcgagcc cttccacacc gacaccgggc gactgcacgc ctacgccgac
     5401 gtcccggagg cgatcgagta tggcgagaac ttcgtcgtcc accgtgaagg cccggaggcg
     5461 accccgtacc ttccgaacgt catcgtctcc tccaacccct tcatccgccc cgagagctac
     5521 gggatcgccg agaccgccga gcactgggac gagcgcacga tccgcaacgt caagctgccg
     5581 tggagtcgcg tgaaggagac gcagggcttc ctctggaagc agggctaccg cttctactgc
     5641 ctcacgccca agacgcgcca ccgcgtccac tccagctggt ccaacgtgga ctggcacatg
     5701 ctgttcgatt cgaacttcgg tgatccctat cgcctcgacc ggcgggcccc gtcggtgggc
     5761 gagcaccagc ttcacatgaa cccgcaggcc gcccgcgacc tgggcatcgc cgacggcgac
     5821 tacgtctacg tggacgcgaa tccggccgac cggccgtacg ccggtgcgaa gcccggcgac
     5881 cccttctacc gtgtggcccg ctgcatgctc cgcgtgaagt acaaccacgc ctatccctac
     5941 cactgcgtga tgatgaagca cgcgccgttc atcgccacgg aaaggagcgt caaggcgcac
     6001 gagactcgcc ccgacggccg ggcgctgtcc gagaacaccg gctaccaggc caacctccgc
     6061 tacggctctc agcagtccgt cacgcgcaac tggcacatgc cgatgcacca gaccgacacg
     6121 ctcttccaca aggccaaggc gttcatgggc ttcatcttcg ggggcgaggc ggacaaccac
     6181 gcgctgaaca ccgtgcccaa ggagacgctg gtgaagatca ccaaggccga ggacggcggc
     6241 ctcggcgggc gcggcgtctg ggcgccggcc acgacgggat tcacacccga caacgagagc
     6301 gacttcatga agcgttacct ggccggcgat ttctaccgga aggcgtgacg atgccgtacg
     6361 gcgagcccga tcccggcgat cccagcgtcc tggtgggcgt gacgcttccg gccggcgccg
     6421 aggcggtgcg ggacatggcc tgggtcttcg ccgaggagtt cgcgcgcctg ggctacgatg
     6481 cgcggcgcat cctcgggctg ttccgctcgc cgttctacgc gggcgcccac cacgcgctgc
     6541 gcgcgctggg cgagaccgag gtcgccgcca tcatcgagga gtgcgtgagc gcctggcgcc
     6601 ggccgccgcg cggtgacgcg acgccgccgg gcggtggcgg acggccaccg cagggagact
     6661 agggcatggc tacggtccac aactggcaac tcggccgcga gatgagctat ccctacgaat
     6721 ccgcctaccc cgagcggcag ttcgccttcg tgttcaacat caaccggtgc atcgcctgtc
     6781 agagctgcac catggcctgc aagtccacct ggacgttctc caagggccag gagcacatgt
     6841 ggtgggccaa cgtcgagacg aagccgtacg gcggctaccc catgttctgg gacgcgaaga
     6901 tcctcgagct gctggagcgc gccaacccgg agggcacgcg ctggaccggc gcgctcgcgg
     6961 acgacccgcg gcagccctac gggcggttcg acgggcggac gatcttcgag gcgacgcgca
     7021 tgctgacgcc cgacagcgcg cgcgtgctcg gctacctgcc gaccgacgcc gagtggaact
     7081 cccccaacat ctacgaggac aacccggtcg gccagcgggg caccccggga gaatatcacc
     7141 ggacgggcga gagcctgccc gagcacaaga cctggttctt ctacctggcg cgcatctgca
     7201 accattgctc gtatccggcg tgcctggccg cctgcccgcg caaggccatc tacaagcggc
     7261 ccgaggatgg catcgtcctg atcgatcagg cggagtgtcg cggctaccgc aagtgcgtcg
     7321 aggcctgccc gtacaagaag tcgatgtacc gcggcaacac ccgggtgtcg gaaaagtgcg
     7381 tcgcgtgcta tccgcgggtc gagggcaagg accccgagaa ccccggcatc cccctggaga
     7441 cccggtgcat ggctgcttgc atcggccaga tccggctcca ggggctcgtg aagctgaacc
     7501 gggacggcac gtgggcggag gaccgccaca accccattta ttacctcgtg cgcgtggcga
     7561 aggtcgcgct gccgctctat ccccagctcg gcaccgagcc gaacggctac tacatcccgc
     7621 cgcgctgggt gccgcggccg tatctgcggc agatgttcgg ccccggcgtc gaccaggcga
     7681 tcgagcgcta cgagaacccg gaccgcgagc tgaccgcggt gctccagctc ttccgccggt
     7741 cgaaccggat catctacggc tacaaggtgg aggacgggcc gaaggtgcac gaggccacgc
     7801 tcagggggcg gaaggtcacc ctctacaacg acaccgtgat cgcctacggc aaggacggca
     7861 acgagatctt ccggacgacg gtggaggagc cggtgcacgt tcgcccgccc cgccacgcca
     7921 actcgatctg aggcgcagag cgccaggaga tgcgatgacg agcagcgggc catccgccaa
     7981 ccaggacccc gtcggagtct cgaggcgcgc cacggctgtg ccggggcgtg ccgagc
//
LOCUS       DATIAZ010000196         6419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1367689, whole genome shotgun sequence.
ACCESSION   DATIAZ010000196 DATIAZ010000000
VERSION     DATIAZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367689"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VLF19_06950"
     CDS             <1..729
                     /locus_tag="VLF19_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSE93027.1"
                     /translation="LDEFKSDRPDDYLAGFPDHPHRGFETVTYMLAGAMRHQDHAGNQ
                     GELVAGSVQWMTAGRGIIHSEMPRQKDGLMWGFQLWVNLPARDKMTAPRYQDIAPEKI
                     PEATLADGVTVRVIAGEASGVRGPVEGIATRPLYLDVRMRARTRASMTLQPGHSAFVY
                     VYDGRAALGTDGGGQEVAAGQLAVLSDGDRLSAATGDAPARFLMLAAMPLHEPIARYG
                     PFVMNTREEIVQAVQDYQRGTLLG"
     gene            complement(776..982)
                     /locus_tag="VLF19_06955"
     CDS             complement(776..982)
                     /locus_tag="VLF19_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HSE93028.1"
                     /translation="MNKDEMGGKWEKAKGKVKETAGEVTGDRDLEAEGVAQQDAGEAQ
                     DAFGKARRKAGEAIEDVGDKIKGE"
     gene            1087..2412
                     /locus_tag="VLF19_06960"
     CDS             1087..2412
                     /locus_tag="VLF19_06960"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSE93029.1"
                     /translation="MLSRDDNELLTRVGPATAMGALIRRYWIPVLKSSELEPGGRVKR
                     VMLLGERLVAYRTKNGRAGLIGEFCPHRLASLYFGRVEDAGMRCVYHGWKFALDGQCL
                     EMPSEPADSAFAAKIRHTAYPCAERGGVIWTYMGPGSARPTLPDLEWTLLPGPQVFIS
                     KRVQECNWFQALEGGIDSSHIGFLHAPLHADDEDVVRELDRASFGVGAAVQTPDRAPR
                     FDVVDTDYGVLIGAVRSRPDGQCYWRVSQFMLPFYTMPPTDLEARIVQSHIWVPMDDT
                     TVVNWMVTWHPDRPLTREELAPHVEGKGSHVIDYLPATSEPYGDIRTADNRGNDYGMD
                     WERHRTTMFCGIPGFGVQDQAIQESQGSVVDRTQEHLGTSDSAIIHVRRRLMNAARAL
                     REQGTPPPGQDPASFLVRSAAVVVPPDADWVQAAMPRIVVRPGQQLVRV"
     gene            2481..3338
                     /locus_tag="VLF19_06965"
     CDS             2481..3338
                     /locus_tag="VLF19_06965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE93030.1"
                     /translation="MRAVRLQQRVGPAGGGHPAEHAEVRRLRPGRLRGRVALITGGGG
                     GIGEATGRLFAEEGASLALVDASAEAVQSTAKAIAGAVESARVLPLVADVSREDETRR
                     VVADVLAHYGALHCLVNVAGVRVYQPLAAASAEDWQTILGVNVLGTAYCCKAAIPALR
                     AVGGGTIVNVSSVYGVMGRKGMGQYDTTKAAVLGMTRALAAEEAPHGIRVNAVCPGST
                     ITPYHIRRAAAQGQSEAELRQRTAGENLLGRWAEPREIAYPILFLACEESSYITGATL
                     MVDAGKSIV"
     gene            complement(3348..3854)
                     /locus_tag="VLF19_06970"
     CDS             complement(3348..3854)
                     /locus_tag="VLF19_06970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93031.1"
                     /translation="MQTWLRVILCCLLAPALLAAGTPGSSIAADTGPSGSSAPVDPDY
                     TSAVNAIKAGSFASAVPLLEGVVKRDDTNADAHNMLAYAIRKNGDPARSIPIYQKALA
                     IDPKHRGAHEYIGEAYLALDDLGKAKEHLKALDKLCFLPCSEYRDLKKAVEVYEKSGG
                     KVKPQATK"
     gene            3930..4958
                     /locus_tag="VLF19_06975"
     CDS             3930..4958
                     /locus_tag="VLF19_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93032.1"
                     /translation="MAEDIEAWFERGVTDGLPVVPPTRARVDAMLATTRRDRRELVAP
                     VPPSFGRATVEKIAINAVMAGCRPDYFPVVLAVVEAACDPVFNLHGQSGTTNAASPLV
                     IINGPVRQRLGVNCAAGVFGPGYRANATIGRALRLVMINLGGSRAGQISMSTMGHPGR
                     YTYCVGEHEEVSPWEPLHVERGFAPTESTVTLFSGESPLMINDHLSRSAAQLVASLGW
                     SAAGVWNHKSFPLYGHTLFVIGPEHAKTLGDERWSKRDVRAYLYETIRRPACDLAPGP
                     DGAETGRLRDELQGRAPQERIPKFPSLEEIVVIVAGGTAGRFSAVVPGWMGGEMGSRP
                     VTRRIDGG"
     gene            5702..5857
                     /locus_tag="VLF19_06980"
     CDS             5702..5857
                     /locus_tag="VLF19_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93033.1"
                     /translation="MLLTIRIRLALALFTGRVGLTLHGLTIRARRAFALFTGRVGLTP
                     RLADHVG"
     gene            6222..>6419
                     /locus_tag="VLF19_06985"
     CDS             6222..>6419
                     /locus_tag="VLF19_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93034.1"
                     /translation="MTRTWRYTVMFVTFTVVRLTTTLLTTRGPPHPAQVATPTNPGRP
                     HHGITGSPQASGAQQSGRPTVR"
BASE COUNT          842 a         2304 c         2350 g          923 t
ORIGIN      
        1 ctcgacgagt tcaagtcgga tcggccagac gactacctgg ccggcttccc cgaccacccg
       61 caccgcgggt tcgagacggt cacctacatg ctggccggcg ccatgcgcca ccaggatcac
      121 gctggcaacc agggcgagct ggtcgcgggc agcgtgcagt ggatgacggc cgggcgcggc
      181 atcatccact ctgagatgcc gcggcagaag gacggcctca tgtggggctt ccagctctgg
      241 gtgaacctgc cggcgcgcga caagatgacg gcgccgcgct accaggacat cgcccccgag
      301 aagattcccg aggcgacgct ggcggacggc gtgaccgttc gggtgatcgc cggcgaggcc
      361 agtggggtgc gcgggccggt ggagggcatc gccacccggc cgctctacct ggatgttcgg
      421 atgcgggcgc gcacgcgcgc gtcgatgacc ctccagcccg gccacagcgc gttcgtctac
      481 gtctacgatg gccgcgccgc gctcggcacc gacggcggcg gccaggaggt cgccgccggc
      541 cagctcgccg tgctgagcga cggcgaccgg ctgagcgcgg ccacgggcga cgcgcccgcc
      601 cgcttcctga tgctcgccgc gatgccgctg cacgagccga tcgcgcgcta cggccccttc
      661 gtcatgaaca cgcgcgagga gatcgtgcag gcggtgcagg actaccagcg aggaacgctc
      721 ctcggctgag cctcggatcc tcgggccccg ccggcagccg ccgcgcgcgc tcgggctatt
      781 cgcccttgat cttgtcgccg acgtcctcga tcgcctcacc tgccttgcgg cgggccttgc
      841 cgaaggcgtc ctgggcctca ccagcgtcct gctgcgccac gccctcggcc tcgaggtcgc
      901 gatcgcccgt cacctcgccg gccgtctcct taaccttgcc cttggccttc tcccacttgc
      961 ctcccatctc gtccttgttc attacggacc tcctgcgagt cttgcgacca ggccgatgag
     1021 agatgcaacg cgaatgccag tgatcgcgat gaatgctagg cttggggcct gacaaggagt
     1081 ccgcgcatgc tgagccgaga cgacaacgag ctgctgacgc gcgtggggcc ggccacggcc
     1141 atgggtgcgc tgatccgccg gtactggatc ccggtcctga aatcgagcga gctcgagcct
     1201 ggcggccgcg tgaagcgcgt gatgctgctg ggcgagcgtc tcgtcgccta ccgcacgaag
     1261 aacggcaggg ccgggctcat cggcgagttc tgcccccatc gcctggcctc gctctacttc
     1321 ggccgcgtcg aggacgccgg catgcgctgc gtctatcacg gctggaagtt cgccctcgac
     1381 ggccagtgcc tggagatgcc gagcgagccg gcggactcgg cgttcgccgc gaagatccgg
     1441 cacaccgcgt acccgtgcgc ggagcggggc ggcgtgatct ggacctacat gggcccgggg
     1501 agcgcgcggc cgaccctgcc cgacctcgag tggacgttgc tgcccgggcc gcaggttttc
     1561 atctccaagc gggtgcagga gtgcaactgg ttccaggcgc tcgagggcgg catcgactcg
     1621 agccacatcg ggttcctgca cgcgccgctg cacgcggacg acgaggacgt cgtgcgcgag
     1681 ctggaccgcg ccagcttcgg cgtgggcgcc gccgtgcaga cgccggaccg cgcgccccgc
     1741 ttcgacgtgg tggacaccga ctacggcgtg ctcatcggcg ccgtccgctc gcggcccgat
     1801 ggccagtgct actggcgcgt ctcccagttc atgctgccgt tctacacgat gccgccgacc
     1861 gacctggagg ccaggatcgt ccagtcgcac atctgggtac cgatggacga caccaccgtc
     1921 gtcaactgga tggtcacctg gcacccggac cgcccgctca cccgcgagga gctggcgccc
     1981 cacgtcgagg gcaagggctc gcacgtgatc gactacctgc cggccacctc cgagccctac
     2041 ggcgacatcc gcaccgccga caaccgcggc aacgactacg gcatggactg ggagcggcac
     2101 cgcacgacga tgttctgcgg catccccggc ttcggcgtgc aggaccaggc catccaggag
     2161 agccagggat cggtcgtgga ccgcacgcag gagcacctcg gtaccagcga cagcgcgatc
     2221 attcacgtgc ggcggcgcct gatgaacgcg gcgcgcgcgc tgcgcgaaca gggcacgccg
     2281 ccgccggggc aagacccggc gtcctttctg gtacgctcgg cggcagtggt ggtgccgccc
     2341 gacgccgact gggtgcaggc ggcgatgccc cgcatcgtcg tgcgcccggg tcagcagctc
     2401 gtccgcgtct gaccgtgcat cgccgacagc gacgtcgttc cggcacgggt cccggccgcg
     2461 ccgtcccggc ccggagagac gtgcgcgcgg tgcgtctcca gcaacgcgtt ggcccggccg
     2521 gcggcgggca tcccgccgag catgcggagg tccgccgtct ccgaccgggg cgcctgcgcg
     2581 ggcgcgttgc gctgatcacg ggtgggggcg gcgggatcgg cgaggccacg ggccggctct
     2641 tcgccgagga gggggcgtcg ctcgcgctgg tggacgccag cgccgaggcc gtccagtcca
     2701 ccgccaaggc catcgccgga gccgtcgaga gcgcacgcgt gctgccgctc gtcgccgacg
     2761 tgtcccgcga ggacgagacg cggcgcgtgg tggccgacgt cctcgcccac tacggcgcgc
     2821 tgcactgcct cgtcaacgtt gcgggcgtgc gcgtctatca gccgctggcc gcggcgagcg
     2881 cggaggactg gcagacgatt ctcggcgtca acgtgctcgg caccgcctac tgctgcaagg
     2941 ccgccatccc cgcgctccgg gccgtcggcg gtgggaccat cgtgaacgtc tcatcggtct
     3001 acggcgtgat gggacgcaaa ggcatggggc agtacgacac gacgaaggcc gccgtgctcg
     3061 gcatgacccg cgcgctcgcc gccgaggagg cgccgcacgg gatccgcgtg aacgccgtgt
     3121 gccctggctc gacgatcacg ccctaccaca tccgccgggc tgccgcccag ggccagagcg
     3181 aggccgagct gcgccagcgg acggccgggg agaacctgct cgggcgctgg gccgagccgc
     3241 gcgagatcgc ctacccgatc ctgttcctgg cctgcgagga gtcgtcctac atcaccggcg
     3301 ccacgctgat ggtggacgcg ggcaagtcga tcgtgtagcg caactcgtca cttggtggcc
     3361 tgaggcttca cctttccgcc gctcttctcg tacacctcga cggccttctt caggtcgcgg
     3421 tactcggagc acggaaggaa gcagagcttg tcgagcgcct tcaagtgctc cttggccttg
     3481 ccgaggtcgt cgagcgccag gtaagcctcg ccgatgtact cgtgggcgcc gcggtgcttc
     3541 gggtcgatcg ccagggcctt ctggtagatc gggatcgatc gggcggggtc gccgttcttg
     3601 cggatcgcgt acgcgagcat gttgtgggcg tcggcattcg tgtcgtcgcg cttcaccacg
     3661 ccctccagaa gggggaccgc cgaggcgaag ctccccgcct tgatcgcgtt cacggcggag
     3721 gtgtagtcgg ggtccacggg tgcggacgag ccggaagggc cggtgtcggc cgcaatcgag
     3781 gagcccgggg tgccggccgc gaggagcgcc ggcgccaaca ggcagcacaa gatcacgcgc
     3841 aaccaggtct gcataaggtg cctcccgtcc tgcgtgccgt gattgtacct ccgcacgcgg
     3901 taggcacacc gcgctgctac gatagcgccg tggccgagga catcgaagcc tggttcgagc
     3961 gcggcgtcac cgacgggctg cccgtggtgc cgcccacccg cgcccgcgtg gacgcgatgc
     4021 tggcgacgac gaggcgcgac cgccgcgagc tcgttgcgcc ggtgccgccg agcttcggcc
     4081 gcgcgacggt cgagaagatc gcgatcaacg ccgtcatggc cggctgccgg cccgactact
     4141 ttcccgtggt cctggcggtg gtcgaggccg cgtgcgaccc cgtcttcaac ctgcacggcc
     4201 agtcggggac gaccaacgcc gcctcgccac tcgtcatcat caacgggccc gtacggcagc
     4261 ggctcggcgt caactgcgcg gccggcgtct tcggccccgg ctaccgggcc aacgcgacca
     4321 tcgggcgcgc gctgcggctc gtcatgatca acctgggcgg ctcgcgcgcg ggccagatct
     4381 cgatgtccac catgggccat cccggccgct acacgtactg cgtcggcgag cacgaggagg
     4441 tctcgccgtg ggagccgctg cacgtcgagc gcggattcgc cccgacggag agcacggtga
     4501 ccctcttctc cggcgagagc cccctcatga tcaacgatca cctgagccgc tcggcggcgc
     4561 agctcgtggc ctcgctcggc tggtccgcgg cgggcgtctg gaaccacaag agctttcccc
     4621 tctacggcca cacgcttttc gtgatcggac cggagcacgc gaagacgctc ggcgacgagc
     4681 ggtggtcgaa acgggacgtg cgggcgtacc tctacgagac gatccgccgg ccggcctgcg
     4741 acctggcgcc cgggcccgac ggcgccgaga ccggccggct gcgggacgag ctgcagggcc
     4801 gcgcgcccca ggagcgcatc ccgaagttcc cctcgctcga ggagatcgtc gtcatcgtgg
     4861 cgggcggtac ggccggacgg ttctcggcgg tcgtccccgg ctggatggga ggcgagatgg
     4921 gctcgcgtcc ggtgacgcgg aggatcgacg gcggctagcg tcgcggggga gcctgctcgt
     4981 cgccctgctg gtgcgcgccc gggccgggcc tgggattgcc gcgtgtctgc ggccgcggtc
     5041 tcttctcgcc gcccgggccc tggccggctt gctgaggccc tccggccgcc ggcggctcgg
     5101 ccacggaggg ctgctgggta ggcggaacgg gatgcggcgg catcgcctcc gaggcgcggg
     5161 cgggaggctc cggcgcgcgg ggctgcgcgg cgtgcggcag agccccacgc ggccctgtcg
     5221 ggtgttgctg ctgtgtggcc cgcggcgcag ggggtgccgg cacggggtcc gacgattctt
     5281 gccggcgccg gaccgtgggc gactccggcg gtaccgttcg ttcgatcgca cgtggtgtca
     5341 ccggcggcgg cccggcggcg gggcgctcga tcgattgcgg tggggccggc ggcgcagcgg
     5401 ccggacgatc gacgacgcgt ggcgcagtcg ctggattctg gggctggggc gccggcacag
     5461 ccggacgggg cgcgaccgca ggcggcgtga gcgcgggccg agtcgggccg acagccggtg
     5521 tttcgggcgg cgtagtgcgg tcgctgcgcg cgggcggttc gctcgacggc gcccgcctcg
     5581 gagtggggcg cggaggccgg ccggcatcgg gtgagtcctg ctgctgcaag cggccggccg
     5641 gctcctgcga gcgcgccgct ggcggtcggg gaccctgctg gccctcgccc ggccgacgcc
     5701 catgctgctc acgattcgca tccgcctggc gctcgcgctg ttcacggggc gcgtcggact
     5761 gacgctgcac gggctcacga ttcgcgcccg ccgggcgttc gcgttgttca cggggcgcgt
     5821 cggactgacg ccccggctgg ccgatcacgt cgggtgagcg ctcgtctcgc acgcggcccg
     5881 cggcgggcgg gctcacgcgt gctggagtcg cgcgccgcgc cggcggagca accacgcggg
     5941 tgctccgcgc gggcgcctcg gccgtcgtcg cgggcaagcc ggccgcgcgc agcgacggag
     6001 acggatcgtg cggcttgcgt gtggccacca cctcgcgccg gtgagtcatg accggcggcg
     6061 cgataccgcg ctcacgcgtg ggcgcgacgc tggccgcgac gggctgcact ctcggcgcgc
     6121 cgcgcacagg ccggagctgc tgcacctctg tcgcgctgat gcgcgcgggg cgagcatggt
     6181 cgcgaccgaa acgctccggc ggcacgccga cgacggcctt cgtgacccgc acgtggcggt
     6241 acacggtgat gttcgtcacg ttcaccgtcg tccggttgac gaccacgttg ttgaccacgc
     6301 gcgggccgcc ccatcctgcc caggtcgcca cgccgacgaa ccccggtcgg ccccaccacg
     6361 ggatcaccgg ctcaccccag gcgagcggcg cccagcagag cggacggccc accgtgcgc
//
LOCUS       DATIAZ010000197         4677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_315188, whole genome shotgun sequence.
ACCESSION   DATIAZ010000197 DATIAZ010000000
VERSION     DATIAZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315188"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VLF19_06990"
     CDS             complement(<1..518)
                     /locus_tag="VLF19_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93035.1"
                     /translation="MRVLVVEDEPDVSAIFRDYLVELGHEPVLVRSAEAALRRLESDR
                     PDAIILDIHLPGISGIEFLKLRSVRESGLPIVAVSGMATESQAREILRLGAFDFVGKP
                     VPFQRLGEVLAYIEPHALHRQLADVGKSPERRRGDRVRVTFPVRVSTYDGGEWEGWAF
                     DLSPFGMRVRGDV"
     gene            complement(712..1251)
                     /locus_tag="VLF19_06995"
     CDS             complement(712..1251)
                     /locus_tag="VLF19_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93036.1"
                     /translation="MSPGGHLLTTVAACASAAATTGAPEIAAGIALGGFLIDIDHAVD
                     YVFFEGQRDLRPGAFLRYYMEGRARRTVLLLHSYELLVVLAWLAWSLDSGLLVGYVFG
                     AVMHLGLDLRYNGEYTPRSIAAFYSFGYRLVHRFDAGTLLAYAAPPVAWTGFWGTFFA
                     GIRRRGPGTLRRPAPAPRA"
     gene            1328..1702
                     /gene="ndhC"
                     /locus_tag="VLF19_07000"
     CDS             1328..1702
                     /gene="ndhC"
                     /locus_tag="VLF19_07000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSE93037.1"
                     /translation="MLYSDPVLSGYLPVVIFAALIVAFGVVSLGAARLLRPSRPDAVK
                     LMNYECGAEPIGSAWVRFPVGFYLVALVFIVFDTLAVFLFPWTLILGSAGMAAFWVVA
                     AFVGIVGLGWVYAYREGALEWR"
     gene            1843..2331
                     /locus_tag="VLF19_07005"
     CDS             1843..2331
                     /locus_tag="VLF19_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSE93038.1"
                     /translation="MPGGWILVTSTDKVFNWARSSSIWPVTFGLACCAIEMMATFASR
                     FDVERFGMVPWASPRHSDLMIVSGTVTIKMAPMLKRIYDQMPDPKWVVSMGSCANSGG
                     PFRHGYHVVKGVDRVVPVDVYVPGCPPTPDSLMFGLLRLQERVSEFQKTARREPVLAS
                     EG"
     gene            2339..2788
                     /locus_tag="VLF19_07010"
     CDS             2339..2788
                     /locus_tag="VLF19_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSE93039.1"
                     /translation="MTADAVARVREAFGVDAVTDGTVVTVALATDRWPAAARIARDRL
                     GCLYFSWLSAVDWKDQGLEVVCRVENLEAGLALLLRTRLGAGVTRCPTLTGVWRGADW
                     MERECYDMFGVVFEGHPDLRRILLGDDWEGHPLRKDYAVDTPHAPYR"
     gene            2802..3437
                     /locus_tag="VLF19_07015"
     CDS             2802..3437
                     /locus_tag="VLF19_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HSE93040.1"
                     /translation="MIHELRTYTLHPGKQPEYLKLNAEVGRKVRGDRYGKFEGGWTTE
                     FGTLNQYVHLWAYSSLDERTRLRGELAKTDEWTKGYVPQIRPLIAAQETKVLTPVLPL
                     QPPQDRGNVYELRWYRTQTGRSGEWLELFKAVMPAREKYMRRVGLWQTDLGPLNEVVH
                     LWVFRDLNERAEARARLGQDPAWQKFVAASAPLLAHMQAAVLIPTPASPMA"
     gene            3533..>4677
                     /locus_tag="VLF19_07020"
     CDS             3533..>4677
                     /locus_tag="VLF19_07020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195370.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSE93041.1"
                     /translation="MDRQTVEIGYGGNERLMMNMGPQHPSAHGVFRAILTLEGETVVA
                     VDAVIGYLHRCHEKLGESLTYVQYPTIASKTDYVSAMTAELAYVAAVEKVGKIDVPRR
                     AQYLRVLVAELQRIASHCLWLGTWCMDMGGALGGGATIFLYCMRERESILDLFEALTG
                     ARLLYGFHQVGGTRYDLPAGWAERCRATVDVIEGRIGEYEAMLEDNAFFLARTRAVGV
                     VSAALAQEVGISGPLIRGSGIEYDVRRAEPYSSYGDFDFKIPVETAGDCHARYRVRMR
                     EFRESITIVRQALGGLPEGPISSRPGVKSVGQVRIPKGEGYARVESARGEVGLYLIAD
                     GGPKPYRMKWRGPSFSNLAILPHIIPGHKVADVVAIMGSVDPVFGEV"
BASE COUNT          710 a         1573 c         1693 g          701 t
ORIGIN      
        1 acgtcgccgc gcactcgcat cccgaacgga ctcaaatcga aggcccagcc ctcccactcg
       61 ccgccgtcgt aggtggacac gcggaccggg aaggtgacac gcacgcggtc gccgcgcctc
      121 cgctccgggc tcttgcccac gtcggcgagc tgccgatgca gggcatgggg ctcgatgtag
      181 gcgagaacct cgccgagacg ctggaacggc acgggcttgc cgacgaagtc gaaggcgccg
      241 agacgcagga tctcgcgcgc ctggctttcg gtcgccatgc cggagaccgc gacgatgggc
      301 agacccgact cccgcacgga gcggagcttc aggaactcga tgccggaaat gccgggcagg
      361 tggatgtcga ggatgatcgc gtccgggcgg tcgctctcga gccggcggag cgccgcctcg
      421 gcgctccgca cgagcacggg ctcgtggccc agctcgacca ggtagtcgcg gaagatcgca
      481 ctgacgtccg gctcgtcctc aacgacgaga acgcgcatcg caagtgcgag tctagcacgc
      541 agccgcgcgc gctgactagg caccgacgcc acatcgaggc tgcgggatgc ggcactcgga
      601 gcgtttgatt ggagcgtgcc accgcgtgaa ccggggcgcg ccggacgtca ggatcgagac
      661 ggaccgaggt cgtgccgaac gtgggggggg gccgccgggg ccccgactct cctacgcgcg
      721 cggcgctggg gccggccggc gtagcgttcc gggtccgcgg cgtcggatcc cggcgaagaa
      781 cgttccccag aagccagtcc aggcgacggg gggagccgcg taggcgagca gggttcccgc
      841 gtcgaaccgg tggacgaggc ggtagccgaa gctgtagaag gcggcaatgg agcgcggcgt
      901 gtactcgccg ttgtaccgga gatccagccc gaggtgcatc accgcgccaa agacgtaccc
      961 gacgagcagc ccggagtcga ggctccaggc gagccaggcg agcacgacga gcagctcgta
     1021 cgagtgcagc aggagcacgg tgcgccgcgc gcggccctcc atgtaatatc gcaggaacgc
     1081 gccgggacgt agatcacgct ggccctcgaa gaagacgtag tcgacggcgt ggtcgatgtc
     1141 gatgaggaag ccgccgaggg cgatccccgc ggcgatttcc ggagcaccgg tggtcgccgc
     1201 ggcggacgcg cacgcagcga cggtggtcag gagatgccct ccggggctca cggttccatc
     1261 gtaccagacg ccgggcgcgg tcctgtcgag tgcccgcgag accggcggcc gcgtggtcag
     1321 accgcgcatg ctatactcgg atcccgtgct gtcgggatac ctgcccgtcg tgatcttcgc
     1381 cgcgctgatc gtcgccttcg gcgtggtgtc gctcggcgcg gcgcgcctgc tgcggccgtc
     1441 gcgtcccgac gccgtcaagc tgatgaacta cgagtgcggc gccgagccga tcggctcggc
     1501 gtgggtgcgg ttcccggtgg gcttctacct cgtcgcgctg gtcttcatcg tcttcgacac
     1561 gctggccgtg tttctgttcc cctggacgct gatcctcggc tcggccggca tggcggcgtt
     1621 ctgggtggtc gccgccttcg tggggatcgt ggggctcggc tgggtgtacg cctaccgcga
     1681 gggagccctc gaatggaggt gaggccgccg gtgccgcagg taccgcctcc cgtctggggg
     1741 gacgtcaagg acctgcagga gtgggagaag tatcaccagg agcggcccga gggcgacaag
     1801 acctcgagca cgctggcggc gatcgccgac gccatccatc acatgccggg cggctggatc
     1861 ctcgtcacct ccaccgacaa ggtcttcaac tgggcgcgca gctcgtcgat ctggccggtg
     1921 acattcggcc tggcgtgctg cgccatcgag atgatggcga cgttcgcctc gcgcttcgac
     1981 gtggagcgct tcggcatggt gccgtgggcc tcgccgcggc actccgacct catgatcgtg
     2041 tcgggcacgg tgaccatcaa gatggcgccg atgctcaagc ggatctacga ccagatgcct
     2101 gaccccaaat gggtcgtgtc catgggctcg tgcgcgaact cgggcggccc gttccgccac
     2161 gggtatcacg tggtcaaggg cgtggaccgc gtcgtgcccg tggacgtcta cgtgcccggg
     2221 tgtccgccca cgcccgattc gctcatgttt ggtctgctca ggcttcagga gagggtctcg
     2281 gagttccaga agacggcgcg gcgcgagccg gtgctcgcgt ccgagggctg atccggccgt
     2341 gaccgccgac gcggtggcgc gagtccggga ggcgtttggc gtcgacgcgg tcaccgacgg
     2401 caccgtcgtg accgtggcgc tggcgaccga ccgctggccg gcggcggcgc gcatcgcccg
     2461 ggaccggctc ggctgtctct acttctcgtg gctctccgcc gtggactgga aggaccaggg
     2521 tctggaggtg gtctgccgcg tggagaacct cgaggcgggc ctcgccctcc tgctgcgcac
     2581 gcgcctcggc gccggggtca cgcgctgccc gacgctgacc ggcgtctggc ggggcgccga
     2641 ctggatggag cgcgagtgct acgacatgtt cggcgtggtc ttcgagggcc atcccgacct
     2701 ccggcggatc ctgctcggcg acgactggga aggccacccg ctgcgcaagg actacgcggt
     2761 cgacaccccc cacgccccgt atcgctgacg agggacgcgc catgatccac gagctgcgga
     2821 cctacacgct tcaccccggc aagcagcccg agtacctcaa gctcaacgcg gaggtcggcc
     2881 gcaaggtgcg cggcgaccgc tacggcaagt tcgagggagg ctggacgacg gagttcggca
     2941 cgctcaacca gtacgtccac ctctgggcct attcgagcct cgacgagcgc acgcggctgc
     3001 ggggcgagct ggcgaagacc gacgagtgga ccaagggata cgtgccccag atcaggccgc
     3061 tgatcgccgc gcaggagacg aaggtgctca cgccggtgct gccgctccag ccgccccagg
     3121 accgcggcaa cgtgtacgag ctgcgatggt atcgcacgca gaccgggcgg tccggcgagt
     3181 ggctcgagct cttcaaggcc gtcatgccgg cgcgcgagaa gtacatgcga cgggtgggcc
     3241 tgtggcagac cgatctcggg ccgctgaacg aggtcgtcca cctctgggtg ttccgcgacc
     3301 tcaacgaacg cgcggaggcg cgggcgcgcc tggggcagga ccccgcgtgg cagaagttcg
     3361 tcgccgcttc ggcgccgctg ctggcccaca tgcaggccgc ggtgctgatc ccgacgccgg
     3421 cctccccgat ggcgtgaccg agcgcgacat tcttccgacc gtcgggccgc cgtgcgatgc
     3481 gagcggcgaa acgacccgag atcgcgctcc gacggcgaac gtgaaggatc tgatggaccg
     3541 ccagaccgtc gagatcggct acgggggcaa cgagcgcctc atgatgaaca tggggccgca
     3601 gcatccgtcc gcccacggcg tgttccgcgc gatcctcacg ctcgagggcg agaccgtggt
     3661 cgccgtggac gccgtgatcg gctacctgca ccgctgtcac gagaagctcg gggagagcct
     3721 cacctacgtg cagtatccga ccatcgcctc gaagacggac tacgtgtcgg ccatgaccgc
     3781 cgagctggcc tacgtggccg cggtggagaa ggtcggcaag atcgacgtgc ccaggcgcgc
     3841 ccagtacctg cgcgtgctgg tggccgagct ccagcgcatc gcctcgcatt gtctctggct
     3901 cggcacctgg tgcatggaca tgggcggcgc cctcgggggc ggggccacga tcttcctcta
     3961 ctgcatgcgc gagcgcgaga gcatcctcga cctcttcgag gcgctcaccg gagcccgcct
     4021 gctctacggg ttccaccagg tgggcggcac gcgttacgac ctgcccgccg gctgggccga
     4081 gcggtgccgc gccacggtcg acgtgatcga gggccgtatc ggcgagtacg aggcgatgct
     4141 cgaggataac gcgttctttc tggcccgcac gcgggcggtg ggcgtcgtgt cggcagcgct
     4201 cgcgcaggag gtcggtatct cggggcccct catccgcggc tcgggcatcg agtacgacgt
     4261 gcggcgtgcc gagccgtact cgtcgtacgg cgacttcgac ttcaagattc cggtggagac
     4321 cgcgggggac tgtcacgcgc gctaccgcgt gcgcatgcgc gagttccgcg aatcgatcac
     4381 gatcgtgcgc caggcgctcg gaggcctgcc cgaagggccg atctcgtcgc ggcccggcgt
     4441 gaagtccgtc ggccaggtgc ggatccccaa gggggaaggc tacgcgcgcg tggagagcgc
     4501 gcgaggggag gtggggctct acctgatcgc cgacggcggg cccaagccct accgcatgaa
     4561 gtggcgcggg ccctcgttct ccaacctcgc gatcctgccc cacatcatcc ccggccacaa
     4621 ggtggccgac gtcgtcgcca tcatgggctc ggtcgacccg gtcttcggcg aggtgga
//
LOCUS       DATIAZ010000198        14698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_315306, whole genome shotgun sequence.
ACCESSION   DATIAZ010000198 DATIAZ010000000
VERSION     DATIAZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14698
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315306"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VLF19_07025"
     CDS             complement(<1..444)
                     /locus_tag="VLF19_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSE93042.1"
                     /translation="MRPAHRARGRSAEAQRLLDLLNALGSTTFRQLLWQRASELDLTY
                     AQSQVLFHVADHPGCYMGEVAKAFGVTLPAVTHIIDRLEQKAFVVRGDHPADRRVYVL
                     ELTPQGRALVQELHTMQMRGVEDVLARMAAEDRERVIRGLEALVDA"
     gene            521..1750
                     /locus_tag="VLF19_07030"
     CDS             521..1750
                     /locus_tag="VLF19_07030"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HSE93043.1"
                     /translation="MPRFNEHYLNLRASYLFAEIRRRTKAFAETHPGVRLIDLGVGDV
                     TRPLPPAVVRALHEAADEMGRAETFKGYGPYVGYEFLRAEIATHEFGARGVTVGVDEV
                     FVSDGGKSDSANLQEIFAADCVVALMDPVYPVYADSNVVAGRSGPADESGRHEGMVYL
                     ACTAENGFQPALPDRHVDLVYLCYPNNPTGAVATRPALARWVEYARAHAAVILYDAAY
                     EAYIRDPQIPHSIYEIDGAREVAIECRSFSKNAGFTGLRLAYTVVPKEALARSRAGTT
                     MALNALWTRRMAIKSNGPPYVIQKAAAAVYTPDGRKQVRALVDDYMENARIIGEGLAA
                     AGFTVYGGRHAPYIWGRTPGGVSSWDFFDALLTRAHVVGAPGSGFGPSGEGYFRLTAF
                     GSREQTEEAIERIRTRL"
     gene            complement(1851..2588)
                     /locus_tag="VLF19_07035"
     CDS             complement(1851..2588)
                     /locus_tag="VLF19_07035"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93044.1"
                     /translation="MAFVTGASYGIGAATALGLARDGFDVAVSELRPEHLADTVAGIG
                     KAGARGVGVALDVRSLASIRQALAEVVAAFGRLDVLVNNAGVPLTRPALEVTEAEWDE
                     VVGVNLRGAFFTSQQMGRHLIESGRAGAIVSIASTHGVIGVPDRSTYGISKAGIVQMT
                     RMLAIEWAPHRIRVNAVAPATVETPSRAVAFAANPGRREFLIERIPLRRFGTAEEMAD
                     AVCYLASDRAAYITGQTLLVDGGLTAY"
     gene            2686..3927
                     /locus_tag="VLF19_07040"
     CDS             2686..3927
                     /locus_tag="VLF19_07040"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSE93045.1"
                     /translation="MSAATPHRAPVTRRFTEQRWLLDNIIRSVGMDWDQPRSLYLSAP
                     CGVEANADFAALRTRITKLADASPAFEAVARRREAKAQAAEQDGHAVTARENYFMAAI
                     HWGAAQWPIDENDEQNRFYNGRKRECYTKYAAVADHHVEAAWIPLPGGPSLPAWFHLP
                     PGYRGGRVPVVISLPGMDSFKEMGVAMYGDRWLSRGMAVLAVDGPGQYESPVLGIFFS
                     MPAWIATGTALVEWLAARPEIDPDRIGLAGNSFGSFFGTVAAAHEPRIRAVAVSAVCH
                     EPGFHTIFEEASPTFKMRFMYMSGIGDEAEFDRFRKGMTWEGHADRIRAPYLCVAGEA
                     EELSPLEHTERLLRALQGPKRFVVYQDSRHSVGNVPAANLGPFPPILIADWMASTLDG
                     KPFPSEKWYVEASGRVVKTPM"
     gene            complement(4098..4847)
                     /locus_tag="VLF19_07045"
     CDS             complement(4098..4847)
                     /locus_tag="VLF19_07045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HSE93046.1"
                     /translation="MRLRDKVVIVTGGAKGIGRAYCLSTAAEGARVVVADIVDPKPTV
                     TAIEARGGQALGVECDVSREQDTRRLATETLARFGRIDVLVNNAALYAPLKRRSFMEI
                     PVEEWDQVMAVNLRGLFLCARAVFPAMKAQGKGKIINISSSTFFKGVPDYIHYTTSKG
                     GVVGFTRSLARELGECGIRVNAIAPGFTLSGENEKNISEERKQGNIQMRMLKRAEVPE
                     DLVGTMIFLASDDSDFITGQTLLVDGGASVH"
     gene            complement(4917..5015)
                     /locus_tag="VLF19_07050"
                     /pseudo
     CDS             complement(4917..5015)
                     /locus_tag="VLF19_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(5295..5855)
                     /locus_tag="VLF19_07055"
     CDS             complement(5295..5855)
                     /locus_tag="VLF19_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HSE93047.1"
                     /translation="MALWDPPRREVPDAVALCARAGIRVVVVTGDYGLTAQEVARRIG
                     LRAEKVVTGDEVDRATPETLRALSRQPGVLFDATGDLYRRATTMTLAGIVAAQVGNVF
                     ACRTARESVFRTGQFDDRMVLLGVAVEIALTLALIGAPPLRQVFGLAPLAPPEWTVLL
                     SFPLVVLGLEEARKWIARARARQRGP"
     gene            complement(5771..7762)
                     /locus_tag="VLF19_07060"
     CDS             complement(5771..7762)
                     /locus_tag="VLF19_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSE93048.1"
                     /translation="MLRAPAVTSLLAGDPSSSHAPTVAALLAEQGPSEWGAASALTLA
                     PGVALLHALGAGPAALELRWIGLTRAVDGTRAAIVLGGSLRDAGLALHLVGRLRAGLA
                     DTARLERALEARTLEELVQALAPLDRASDESSLTASEVLALVGSSPHGLDAAEAERRL
                     ATCGTNALEHVRRRPLAMRLLEQLVSFFAVLLWVGGVLALLAGMPELGWAIFAVIVVN
                     GIFSFLQEFRAERAIEALRSLLPREIAVVRDGRPARLPVERIVPGDLVRVTEGDQAPA
                     DGRLLESEDLRVDQSAHTGEARAVFKHPGVDGPPALAGVYERPERVFAGTAVVAGAGL
                     YVATATGMASEIGRVAQLTQAVAAGPSPLQREMARVTRIVTAVAVIVGGSFFGLGAAT
                     GLLSIADSFVFALGVIVANVPEGLLPTLTLSLALAVQRLARRGALVKRLSAVEALGAA
                     TVICTDKTGTLTEGRMVLRAVWVGGGRRAAGTIDATDPDVRSLFEAAVLASQATADHG
                     DPTEVALVAAAAGLGIDAADLRETRPVRAAYAFDSFRKRMTLVRAVGADAVAHVKGAP
                     RETLVLCDHVRIAGPRCRSTPRGARRSWPTTTGSLPRDSGCWRSRRDRCHARTWVRRR
                     GGSSAGSRCSGSWRCGTRRVARCRTPSPSARARASGSWS"
     gene            7987..9132
                     /locus_tag="VLF19_07065"
     CDS             7987..9132
                     /locus_tag="VLF19_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HSE93049.1"
                     /translation="MKRSVDRILTTHTGSLPRPEDLTRTMFAREEGVPVDPAALAARI
                     RSAVTEVVGKQVQAGLDIVDDGEYSKPSYATYIKDRLQGFGGASHPLQYRDLVDFPGM
                     ARRVFGDPGRARRKTPACTGPVSLRDADAARADVANLQAGLAQSGGAREGFLTAASPG
                     VISLFFRNDHYPSHEAYLFAIAEAMKHEYQTVAKAGLVLQVDCPDLAMGRHIQFADLS
                     VEEFRKMARLHIEALDHALAAVPPDQSRVHLCWGNYEGPHHHDVPLADIVDIVFTARP
                     NGISFEAANPRHAHEWRVFERVKLPAGKVIIPGVLDSTTNFIEHPDLVAERITRYARL
                     VGRENVIAGTDCGFGTWVGQAAVDPDIVWAKLASLAEGARRASRELW"
     gene            9162..9491
                     /locus_tag="VLF19_07070"
     CDS             9162..9491
                     /locus_tag="VLF19_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSE93050.1"
                     /translation="MTTPAPTTSARDEVRPVVPKLIELSEKVLYGDVWERPGLSKRDR
                     SLITVAALTAMYRGDQLKGHIERALANGVTREEIGEAITHMAFYAGWPTAMTAARIAK
                     SVFDQRK"
     gene            9526..10464
                     /locus_tag="VLF19_07075"
     CDS             9526..10464
                     /locus_tag="VLF19_07075"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93051.1"
                     /translation="MKVGFIGVGTMGASMASNIQKGGFELVVNDVRREAAAPHLAAGA
                     TWADTPRKVAEASEVVLTSLPGPPEVEAVALGDDGLLAGMKAGKAYFDLSTSSPTLIR
                     RIHKVFGERGIHVLDAPVSGGPRGAKSRKLAIWVGGDEAVFLRHKPVLDAMGDQVIRV
                     GPIGAGSVAKLVHNCAGYAVQCVLAEVFTMGVKAGVEPLALWQAVRQGANGRQRLYDR
                     LTDNFLRGKYDPPAFALRLAHKDVSLATALGREHRVPMRLADLALQELTEALNRGWGE
                     RDSRVAMLLQEERAGVQIKVDEAKLDEALRKEPPLV"
     gene            10584..12044
                     /locus_tag="VLF19_07080"
     CDS             10584..12044
                     /locus_tag="VLF19_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE93052.1"
                     /translation="MRSLTVVLAVMLVLGVSAAAWGQPVPGATVEERAINGAKAYLQK
                     HNLKNVEQTILLSSLYNNSFPDFSERWEKLTGIKIKIVPLGYTDIPAKIMAEAVAKTG
                     QFDMFNDFPYAAPDAAGAGVIVPLDAYAERGKPDFSGVAPGLRYQQNYQGKLYIFVLD
                     GDHLILVLRKDLLDNPQVKAEYKAKFNKEPGCPATMDEWEQMAAFFHTKPGQSRWGMT
                     FDKGLYGAMGYRSINFSYRHFPAYFGGLLFDRDMNPRINTPHGVQAIKQFASAVKYMP
                     PDIQGWGTPQIYPFWASGQAFSVMSFPSIVGFGNGNEKSVIKGKQLSCLIPQTKGFDG
                     KLVRRSPQAAGTGYMVSKYGKHPELAYWFIQWLTGPTVGDEAIAHPKGFWDPFRESNL
                     KNAAIRQRFGEQFLATTMENSKYATSLLMLEGNYEYFNVLDKNLADVMNNNASAEEAA
                     KRIEAGWNKVTNDIGRSRQVQSWRQGVESGIYLDRF"
     gene            12147..13121
                     /locus_tag="VLF19_07085"
     CDS             12147..13121
                     /locus_tag="VLF19_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSE93053.1"
                     /translation="MAEARATRAAAAPAGPGGTVAAWLARERVFRLIPFVLVEGMFLL
                     LLAVPFALTVYISLLKWRANRPFEQAYFEGLGNYTDVLTDVEFWWALARTFYFAGVAV
                     ALELVIGFVLAMLVAQRVRGRRLYMTVFLVPMMVVPVVVGYNFSMIYLDSGPLNQLLW
                     PLTSLLGVEPRIRWLSHPIAAQWAIILADVWQWTSLTFLIFLSGFAALPQPLVNAARV
                     MGASRWQIFRRVQLPLLKPVIVIAVVIRGMEALKIFDQSVLLTFGGPGNSTQTVAFYL
                     WRQVWQFNKFSFGAAASMLLLVMFAVLIYAGIYLLVRQRSTVLKEGRA"
     gene            13118..14077
                     /locus_tag="VLF19_07090"
     CDS             13118..14077
                     /locus_tag="VLF19_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSE93054.1"
                     /translation="MSAGARPRPHAVRARGRLTRRLFPYARHAALLAWSSFVLFPIFW
                     MVSTSFKESWEWVAWPPHWLPREPTLQSYRQIFTFSEMGRELARGATDQVFAIWGAVA
                     DSLVICTLSSLASLLLGTFLAYSVSRFGVGGKNFRYAILTIRMVPPIVIAIPILIYYA
                     IMIPHTTTVLFGARASLIDTYLGLGLLYTATTLPFVIWMMLSFIDEVPYTLEHAARLM
                     GAGRLATIRRVVLPLVASGMVVTFLFIFILNWSEFLLALTLTHPKVTTLPVLLNKFQS
                     AVEGRLYGPQAAIGTVITVPVVVLGILIQKHLVKGFSFGMIRK"
     gene            14074..>14698
                     /locus_tag="VLF19_07095"
     CDS             14074..>14698
                     /locus_tag="VLF19_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSE93055.1"
                     /translation="MSRILLRSLVKTFGGVTAVAGVSLEVRDGEFVVFVGPSGCGKTT
                     TLNMISGLETPTSGEVVIGDLVVNDVEPGERGLGMVFQDLALFPHMTVFENIAFGLRV
                     KRVAEAEVRQRVQEAAATMRIAALLAKKPAQCSGGEAQRVALARTIVTKPSVFLMDEP
                     LSSLDAKLRVEMRTELKRLHGALEATFVYVTHDQAEAMTMADRIVVMD"
BASE COUNT         2227 a         5241 c         5079 g         2151 t
ORIGIN      
        1 cgcgtccacc agcgcctcga ggcccctgat cacgcgctcg cgatcttccg cggccatgcg
       61 ggccagcacg tcctcgacgc cccgcatctg catcgtgtgg agctcctgga ccagcgcccg
      121 gccttgcggc gtcagctcca ggacgtagac gcgccgatcg gccggatgat ccccgcggac
      181 cacgaaggcc ttctgctcga ggcggtcgat gatgtgggtg acggccggga gggtgacgcc
      241 gaaggctttc gccacctcgc ccatgtaaca gccggggtga tcggcgacgt gaaagaggac
      301 ctgcgactgc gcgtaggtca ggtcgagctc cgaggctcgc tgccacagga gctggcggaa
      361 ggtcgtgctg ccgagggcgt tcagcaggtc gagcagccgc tgagcctccg ccgagcgacc
      421 acgagcacga tgtgccgggc gcatgggatc ttgattgtat acgccctaaa caattcgggc
      481 aattaacctc tggactgcgt gtcgaggtac cattggcccg gtgccacggt tcaacgagca
      541 ctacctcaac ctcagggcca gctatctctt cgccgagatc cgacgccgga ccaaggcgtt
      601 cgccgagacg catcccggcg tccgactgat cgacctcggg gtcggcgacg tcactcggcc
      661 gctgccgccg gccgtggtcc gcgcgctgca cgaggccgcc gacgagatgg gacgcgccga
      721 gacgttcaag ggctacggtc cctacgtggg ctacgagttc ctgcgcgccg agatcgccac
      781 gcacgagttc ggcgcccgcg gcgtgacggt cggcgtcgac gaggtcttcg tcagcgacgg
      841 cggcaagagc gacagcgcca acctccaaga gatcttcgcg gcggactgcg tggtggcgct
      901 gatggatccc gtctaccctg tctacgccga cagcaacgtg gtcgccggac gctccgggcc
      961 cgcggacgag agcggccgcc atgagggcat ggtgtacctc gcgtgcacgg cggagaacgg
     1021 cttccagccg gccctccccg accggcacgt cgacctcgtc tacctctgct acccgaacaa
     1081 tcccacggga gccgtggcca cgcgcccggc gctcgcgcgc tgggtcgagt acgcccgcgc
     1141 ccacgccgcc gtcatcctct acgacgccgc gtacgaggcc tacatccgcg acccgcagat
     1201 cccccactcg atctacgaga tcgacggcgc gcgcgaggtg gccatcgagt gccgcagctt
     1261 ctcgaagaac gccggcttca cgggcctgcg gctcgcgtac acggtggtgc cgaaagaagc
     1321 gctggcgcgc tcccgcgccg ggaccacgat ggcgctcaac gcgctctgga cccggcgcat
     1381 ggccatcaag tccaacggcc cgccgtacgt catccagaag gccgcggccg ccgtctacac
     1441 gccggatggg cggaagcagg tgcgggcgct ggtcgacgac tacatggaga atgcgcggat
     1501 catcggcgag ggcttggccg cggcgggatt caccgtgtac ggcgggcggc atgcgccgta
     1561 catctggggc cgcaccccgg gcggcgtgtc gtcgtgggac ttcttcgacg cgctcctcac
     1621 ccgcgcccac gtggtcggcg cgccgggctc gggtttcggc cccagcggcg agggctattt
     1681 ccgcctcacc gcgttcgggt cgcgcgagca gaccgaggag gccatcgaac ggatccggac
     1741 gcggctctga tcggagcccg tggaggaatc gcgctggcga gggcgccacg gctgtgccgg
     1801 gacgtcccga gcgttcgggg aatttggggg gagccggagc ccccgcgtga tcaatacgcg
     1861 gtgagaccgc cgtcgacgag gagcgtctgc cccgtgatgt acgccgcccg gtcgctcgcg
     1921 aggtaacaga ctgcgtccgc catctcctcg gcggtgccga agcgccgcag cggaatccgc
     1981 tcgatcagga actcccggcg tccgggattg gccgcgaagg ctaccgcgcg cgacggcgtc
     2041 tccacggtgg ccggggccac ggcgttgaca cggatccgat ggggcgccca ctcgatggcc
     2101 agcatgcgtg tcatctggac gatgccggcc ttcgagattc cgtaggtcga acggtccggg
     2161 acgccgatca cgccgtgagt cgaggcgatg ctgacgatgg cgcccgcacg accgctctcg
     2221 atcaggtgac ggcccatctg ctggctcgtg aagaacgcgc cccggaggtt cacgccgacg
     2281 acctcgtccc actccgcctc ggtgacctcc agggccggcc gcgtgagcgg cacgccggcg
     2341 ttgttgacga ggacgtcgag acggccgaag gcggcgacca cctcggccag cgcctggcgg
     2401 atgctcgcca gcgagcgcac gtcgagcgcg acccccaccc cccgcgcgcc cgccttgccg
     2461 atccccgcca cggtgtccgc gaggtgctcg ggacgcagct cggacacggc cacgtcgaac
     2521 ccgtcgcgcg ccagccccag cgccgtcgcc gcgccgatcc cgtaggacgc ccccgtcacg
     2581 aaggccaccg gtctgcctgg cgatccctgt tctggcatct ggccctcccc gagtgcctcg
     2641 cagtatagtg ccggcgccac caatcgcgac ccgacggagg agcccatgag cgcggccacg
     2701 ccccatcgcg cgcccgtgac acgccgcttc accgagcagc gctggctgct ggacaacatc
     2761 atccgctcgg tcggcatgga ctgggatcag ccgcgctcgc tgtatctgtc agcgccgtgc
     2821 ggggtggagg ccaacgccga cttcgccgcc ctccgcacgc gtatcaccaa gctcgccgat
     2881 gcctccccgg ccttcgaggc cgtcgcccgc cggcgggagg cgaaggccca ggccgccgag
     2941 caggacggcc atgccgtcac cgcgcgcgag aactacttca tggcggccat ccactggggc
     3001 gccgcgcagt ggccgatcga cgagaacgac gagcagaacc gcttctacaa cggccgcaag
     3061 cgcgagtgct acacgaagta cgcggcggtg gccgaccacc acgtcgaggc cgcgtggatc
     3121 ccgctgcccg gcggcccgtc gctgcccgcc tggttccacc tgccgcccgg ctaccgcggc
     3181 ggccgcgtgc ccgtggtgat ctcgctgccg ggcatggaca gcttcaagga gatgggcgtc
     3241 gcgatgtacg gcgaccggtg gctctcgcgc ggcatggccg tgctcgccgt cgacggtccc
     3301 ggccagtacg agagcccggt gctcggcatc ttcttcagca tgccggcctg gatcgcgacg
     3361 ggcacggcgc tggtggagtg gctggcggcc cggcccgaga tcgatccgga ccggatcggc
     3421 ctcgccggca acagcttcgg gtcgttcttc gggaccgtcg ccgccgccca cgagccgcgc
     3481 atccgcgccg tcgccgtgtc cgcggtgtgc cacgagccag gcttccacac gatcttcgag
     3541 gaggcttcac cgaccttcaa gatgcgcttc atgtacatgt cggggatcgg tgacgaggcg
     3601 gagttcgacc ggttccgcaa gggcatgacg tgggagggcc acgccgacag gattcgggcg
     3661 ccgtacctct gcgtcgccgg cgaggccgag gagctgtcgc cgctcgagca caccgagcgg
     3721 ctgctccgtg cgctccaggg gccgaagcgc ttcgtcgtct atcaggactc gcggcactcc
     3781 gtcggcaacg tgccggccgc caacctcggc ccgttcccgc cgatcctcat cgcggactgg
     3841 atggcgtcga ccctggacgg caagccgttc ccgagcgaga agtggtacgt cgaggcctcc
     3901 ggacgagtcg tcaagacgcc gatgtgagcg cgtcccggtt cgagtcgctc gcgccgtggg
     3961 gaagggcgac gcctccccgg cgcggcgatg acgccgtggc gttcggcccc gagcggcggt
     4021 gagctgggcg tcggtcgacg ccgtccatgt cgctgcgccc ttcggcgccc gacggacgcg
     4081 ctcgccgtgc caccggctca gtgcacgctg gcccccccgt cgacgagcag ggtctggccg
     4141 gtgatgaagt cgctgtcgtc ggaggcgagg aagatcatcg tgcccacgag gtcctccggc
     4201 acctcggctc gcttgagcat ccgcatctgg atgttgccct gcttgcgctc ctccgagatg
     4261 ttcttctcgt tctcgccgct gagcgtgaag ccgggagcga tcgcgttcac gcggatgccg
     4321 cactcgccca actcgcgggc cagcgagcgg gtgaagccga cgaccccgcc cttggacgtc
     4381 gtgtagtgga tgtagtccgg cacgcccttg aagaacgtgg acgaggagat gttgatgatc
     4441 ttgcccttgc cctgggcctt catcgccggg aagaccgccc gggcgcagag gaacagcccg
     4501 cgcaggttga cggccatcac ctggtcccac tcctcgacgg ggatctccat gaaggagcgc
     4561 cgcttcaggg gcgcgtagag ggccgcgttg ttcacgagca cgtcgatgcg gccgaaccgc
     4621 gcgagcgtct cggtggccag ccgccgggtg tcctgctcgc gcgagacgtc gcactcgacg
     4681 ccgagggcct ggccgccgcg cgcctcgatc gccgtgacgg tgggtttcgg atcgacgatg
     4741 tcggcgacga ccacgcgcgc gccctccgcg gccgtgctca gacaataggc ccggccgatg
     4801 cccttggcgc cgccggtgac gatgacgacc ttgtccctga gtcgcattgg ctcctccttc
     4861 tctgggccag acgttcgcgg aacccggcgc agcgccgaga cgatggccgt caccggctac
     4921 gtgctcgccc ggatctcgtc gagcacgggc aggatgtggc gccggaactg ctcggggttc
     4981 tcgctcatcg gaaagtgccc gatctccggc atgatgacgg gccactcgcg tttcaggaac
     5041 ggcccgcgct cgagccgagc gatccggccc tcgatcacgg tgacgagcca ggcggtgtcg
     5101 ccggtctcga cgaggaaggt ggtgctcggg taacggcctc gccgtacctg acgctcgttg
     5161 gcgttgacca ggtcggcgag ctcgtccagc atggcgccct gctcacttca tgtgccgggc
     5221 gagcgcgtcg atggcccggt ccatggcggc gttccggctc atgcgatcct ggatcgctca
     5281 acgggccccc ggtgtcaagg gccgcgctgc cgggccctcg cacgcgcgat ccatttccgc
     5341 gcctcctcga ggcccagcac gacgagcggg aacgacaaca agacggtcca ctcgggcggc
     5401 gccagaggcg ccaggccgaa cacctgacgc agcggcgggg cgccgatcag cgcgagcgtg
     5461 agcgcgatct cgaccgcgac accgagcagg accatgcggt cgtcgaactg acctgtccgg
     5521 aagacggact cgcgggcggt gcggcaggcg aatacgttgc cgacctgcgc cgccacgatg
     5581 ccggcgagcg tcatggtggt cgcccgacgg tacagatcgc cggtcgcgtc gaacagtacg
     5641 ccgggctggc gtgagagcgc acgcagggtc tccggcgtcg cccgatcgac ctcgtcgcct
     5701 gtgaccacct tctcggcccg cagcccgatg cgcctggcga cctcctgggc ggtcaggccg
     5761 tagtcgcccg tcacgaccac gacccggatg cccgcgcgcg cgcagagggc gacggcgtcc
     5821 ggcacctcgc gacgcggcgg gtcccacagc gccacgagcc cgagcagcgt gagcccgcgc
     5881 tcgacccgcc tctgcggcgc acccacgtcc tcgcgtggca ccggtcgcgt cgcgaccgcc
     5941 agcagcctga gtccctcggc agcgagccgg tcgtggtcgg ccaggaccga cgcgcgccac
     6001 gcggcgtcga gcggcaccgt gggccggcga tacggacgtg gtcgcacagc acgagcgtct
     6061 cccgcggcgc acccttgacg tgggcgaccg cgtccgctcc gacggcgcgc accaaggtca
     6121 tgcgcttgcg gaacgagtcg aacgcgtagg ccgcgcggac cgggcgtgtc tcccgcaggt
     6181 cggcggcgtc gatgcccagg ccagcggccg ccgcgaccag cgccacctcg gtggggtccc
     6241 cgtgatccgc cgtcgcctgc gatgcgagga cggcggcctc gaacaagctc cgcacgtcgg
     6301 gatcggtggc gtcgatcgtg ccggccgcac ggcggccgcc gccgacccag acggcccgga
     6361 gcaccatccg gccctcggtg agcgttccgg tcttgtcggt gcagatgacg gtggccgcgc
     6421 cgagcgcctc cacggccgac aggcgcttga cgagcgcccc gcggcgcgcg agtcgctgca
     6481 cggcgagggc cagagacagc gtgagcgtcg gcagcaagcc ttccggcacg ttggcgacga
     6541 tcacgccgag cgcgaagacg aagctgtcgg cgatcgagag gagcccggtg gcggcgccga
     6601 ggccgaagaa cgacccgccg acgatcacgg caacggccgt gacgatccgt gtgacgcggg
     6661 ccatctcgcg ctggagcggg ctcggcccgg ccgcgacggc ctgggtcagc tgggccacgc
     6721 ggccgatctc gctcgccatc cccgtcgcgg tcgcgacgta gagtcccgcc ccggccacca
     6781 ccgcggtccc ggcgaacacc cgctcgggcc gctcgtagac gccggcgagc gccggcgggc
     6841 cgtccacgcc gggatgcttg aagacggcgc gcgcttcacc ggtgtgggcg ctctggtcca
     6901 cgcgaagatc ttcggactcc aggagtcgcc cgtccgccgg cgcctggtcg ccctcggtga
     6961 ctcgaacgag gtcgccaggc acgatgcgct cgacgggcag acgggccggc cggccgtcgc
     7021 gcaccaccgc gatctcgcgc gggagcagcg agcgcagggc ctcgatcgcc cgctcggcgc
     7081 ggaattcctg caagaagctg aagatcccgt tcacgacgat gacggcgaag atcgcccagc
     7141 cgagctcggg catgccagcg agcagggcca ggacgccacc gacccacagc aggaccgcga
     7201 agaagctcac caactgctcg agcaagcgca tggccagcgg gcgcctgcgc acgtgctcga
     7261 gggcgttcgt gccgcacgtg gcgagccgtc gctcggcctc tgccgcgtcg agaccgtgag
     7321 ggctcgagcc gacgagcgcg agcacctcgc tcgccgtcag ggaactctcg tccgacgcac
     7381 gatccagcgg cgccagcgcc tggaccagct cttcgagcgt ccgcgcctcg agcgcgcgct
     7441 ccaggcgcgc ggtgtcggcg aggcctgcgc gcaggcgacc gaccaggtgg agagcgaggc
     7501 cggcgtcgcg aagcgagccg ccgaggacga tggcggcgcg cgtgccgtcg accgcccggg
     7561 tcaggccgat ccagcgcagc tcgagcgccg ccggccccgc gcccagggca tggagcagcg
     7621 cgacgcccgg agccagcgtc agcgccgagg ccgctcccca ctcggaggga ccctgctcgg
     7681 cgaggagcgc cgcgaccgtg ggcgcgtggc tcgagctggg gtcgcccgcc agcagcgagg
     7741 tgacggctgg cgcgcggagc atggcgccgt cattcccccg gcccgaatgc gcgtttgtcg
     7801 tcatccacgg gagcccgcgg caaggtgtgt gccacgggga gtcgccagga aatcgagccg
     7861 ttgggaggag ctcaccggcc agtcgcgagg tcacggcgcc atgggtgggg cgccgtcgcc
     7921 acgacgtccc gcgcctccgc cggcgtgact ctcgccccgc cttgtggtat tcaaggcgcc
     7981 gcccccatga aacggagcgt cgaccgcatc ctcaccacgc acacgggcag cttgcctcgc
     8041 cccgaggacc tgacgcggac gatgttcgcg agagaggaag gcgttcccgt ggatccggcc
     8101 gcgctggccg cgcgcatccg gtcggcggtg acggaggtcg tgggcaagca ggtccaggcg
     8161 ggtctcgaca tcgtcgacga cggcgagtac agcaagccga gctacgcgac ctacatcaag
     8221 gatcgcctgc aggggttcgg gggggccagc catccactcc agtaccggga cctcgtggac
     8281 tttccaggca tggcccgccg ggtgttcggc gaccccggac gcgcgcggcg caagacgcca
     8341 gcctgcacgg gcccggtctc gctgcgtgac gccgacgccg cgcgcgccga cgtggcgaac
     8401 ctccaggccg gcctcgcgca gagcggcggc gcccgcgagg ggttcctgac cgccgcgtcc
     8461 ccgggcgtca tctcgctgtt ctttcgcaac gaccactacc cgagccacga ggcctatctg
     8521 ttcgccatcg cggaggcgat gaagcacgaa taccagaccg tggcgaaggc aggactcgtg
     8581 ctgcaggtgg actgccccga cctggccatg ggacggcaca tccagttcgc cgacctgagc
     8641 gtcgaggagt tccgcaagat ggcgcgcctg cacatcgagg cgctcgacca cgcgctggcg
     8701 gcggtccccc cggaccagtc gcgcgtgcac ctctgctggg ggaactacga gggcccgcac
     8761 caccacgacg tgccgctcgc ggacatcgtc gacatcgtct tcaccgcgcg accgaacggc
     8821 atctccttcg aggccgccaa tccccgccac gcgcacgagt ggcgcgtgtt cgagcgcgtg
     8881 aagctgcccg cgggcaaggt catcattccc ggcgtgctcg actcgaccac caacttcatc
     8941 gagcatccgg acctcgtcgc cgagcgcatc acgcgctatg cgcgcctggt cggccgcgag
     9001 aacgtgatcg cggggacgga ctgcggcttc ggcacctggg tcggccaggc ggcggtcgat
     9061 ccggacatcg tgtgggcgaa gctggccagc ctcgcggagg gcgcgcgccg ggcttcgcgg
     9121 gaactgtggt aggcgacccc gccgcgaaag ggaggagcga cgtgacgaca cctgcgccca
     9181 cgacctcggc gagagacgaa gtccgcccgg ttgtcccgaa gctcatcgag ttgagcgaga
     9241 aggtgctgta cggcgacgtg tgggagcgtc ccgggctgtc caagcgcgac cgcagcctca
     9301 tcacggtggc cgccttgacg gcgatgtacc gcggcgatca gctcaagggc cacatcgagc
     9361 gggccctcgc caacggcgtg acacgcgagg agatcggtga ggccatcacc cacatggcgt
     9421 tctacgcggg ctggcccacg gcaatgacgg cagcgcgcat cgccaagagc gtgttcgatc
     9481 agcggaagtg acacggtcgc cggaggcggc gacgcggagg acagcatgaa ggtcgggttc
     9541 atcggcgtgg gaaccatggg cgccagcatg gcgtccaaca tccagaaggg cggcttcgag
     9601 ctggtcgtca acgacgtccg ccgggaggcc gcggcaccgc acctcgccgc gggcgccacg
     9661 tgggccgata cgccccgcaa ggtcgccgaa gcctccgagg tggtgctcac gtcgctcccc
     9721 ggcccgcccg aggtggaggc ggtggcgctc ggcgacgacg gactcctggc cggcatgaag
     9781 gcgggcaagg cgtacttcga cctgagcacc agctcaccga ccctcatccg gcgtatccac
     9841 aaggtgttcg gcgagcgggg catccacgtg ctcgacgcgc cggtcagcgg cgggccgcgc
     9901 ggggcgaagt cccggaagct ggccatctgg gtcggcggcg acgaggcggt gttcctgcgc
     9961 cacaagcccg tgctggacgc gatgggcgac caggtcatcc gcgtggggcc gatcggcgcc
    10021 ggctccgtcg ccaagctcgt ccacaactgc gccgggtacg ccgtgcagtg cgtgctggcg
    10081 gaggtcttca cgatgggggt gaaggcgggc gtggagccgc tcgcgctgtg gcaggcggtg
    10141 cgccaggggg cgaacggacg ccagcgcctc tacgaccggc tcaccgacaa cttcctgcgc
    10201 ggcaagtacg acccgccggc cttcgcgctg cgcctggccc acaaggacgt cagcctggcc
    10261 accgcgctcg gccgcgagca ccgggtcccg atgcggctgg ccgacctcgc cctgcaggag
    10321 ttgacggagg cgctgaaccg cggctggggc gagcgggact cgcgcgtggc catgctgctg
    10381 caggaggagc gggccggcgt ccagatcaag gtcgacgagg cgaagctcga cgaagcgctg
    10441 aggaaggagc cgccgctcgt ttgactcgga gggggcctcg acggccccct ccgagaccct
    10501 cccccgggag aggattgcgc cggcaaagcc ggcgctcgga gcggtggctc ggacacgctc
    10561 gttcccacag aggagagaag gcgatgcgaa gtctgacggt cgtgctggcg gtgatgctgg
    10621 tgctgggcgt gagtgcggcg gcgtgggggc aaccggtgcc gggggccacg gtggaggagc
    10681 gcgcgatcaa cggggcgaag gcctatctgc agaagcacaa tctcaagaac gtggagcaaa
    10741 ccattctact gtcctcgctc tacaacaact ccttcccgga cttctccgag cggtgggaga
    10801 agctgacggg catcaagatc aagatcgtcc cactgggtta caccgacatc ccggccaaga
    10861 tcatggccga ggcggtggcc aagacgggcc agttcgacat gttcaacgac ttcccctacg
    10921 cggcgcccga cgccgccggc gccggcgtga tcgtcccgct cgacgcctat gcggagcgcg
    10981 gcaagcccga cttctcgggg gtggcgcccg ggctccgcta ccagcagaac taccagggca
    11041 agctctacat cttcgtcctc gacggcgacc acctgatcct ggtgctccgc aaggacctgc
    11101 tcgacaaccc ccaggtgaag gccgagtaca aggcgaagtt caacaaggag cccggctgcc
    11161 cggccacgat ggacgagtgg gagcagatgg cggccttctt ccacacgaag cccgggcaga
    11221 gccgctgggg catgacgttc gacaagggcc tgtacggcgc gatggggtac cggtccatca
    11281 acttctcgta ccgccacttc ccggcctact tcgggggcct gctgttcgac cgggacatga
    11341 acccccggat caacacaccc cacggcgtcc aggcgatcaa gcagttcgcg tcggccgtca
    11401 agtacatgcc gcccgacatc cagggctggg gcacgccgca gatctatccg ttctgggcga
    11461 gcggccaggc cttctcggtc atgtcgttcc cctcgatcgt gggcttcggc aacggcaacg
    11521 agaagagcgt gatcaagggc aagcagctca gctgcctcat cccccagacc aaggggttcg
    11581 acggcaagct cgttcggcgc tcgccccagg ccgccggcac cggctacatg gtgagcaagt
    11641 acggcaagca ccccgagctg gcgtactggt tcatccagtg gctcaccggc cccacggtcg
    11701 gtgacgaggc catcgcccat cccaagggct tctgggaccc gttccgcgag tcgaacctca
    11761 agaacgcggc catccgccag cggttcggcg agcagttcct cgccaccacc atggagaact
    11821 cgaagtacgc cacctcgctc ctcatgctcg agggcaacta cgagtacttc aacgtcctcg
    11881 acaagaacct cgccgacgtg atgaacaaca acgcctccgc ggaagaggcg gccaagcgga
    11941 tcgaggccgg ctggaacaag gtcaccaacg acatcggccg cagccgccag gtccagtcct
    12001 ggcgccaggg cgtcgagagc ggcatctatc tcgatcggtt ctgatcgact gatggggggc
    12061 cccgagatgg ctccccgtac ccccagcttt cggcccgccc cggctttgcc ggggcgggcc
    12121 tctgacacgt caggaggccg gaaggcgtgg ctgaagcgcg cgcaaccaga gcggcggccg
    12181 ctccggcggg cccgggcggc acggtcgccg catggctcgc gcgggagcgc gtgttccggc
    12241 tgatcccgtt cgtcctcgtg gaggggatgt tcctgctcct gctcgcggtg cccttcgcgc
    12301 tcaccgtcta catcagcctg ctcaagtggc gagccaaccg cccgttcgag caggcgtact
    12361 tcgaggggct gggcaactac accgacgtcc tgaccgacgt cgagttctgg tgggcgctgg
    12421 cgcgcacgtt ctacttcgcg ggcgtcgcgg tggcgctcga gctggtgatc ggcttcgtgc
    12481 tggccatgct cgtggcccag cgcgtccggg gtcggcgact ctacatgacc gtgttcctcg
    12541 tcccgatgat ggtggtgccc gtggtcgtcg gctacaactt ctcgatgatc tacctcgact
    12601 ccgggccgct caaccagctg ctgtggccgc tgacctcgct gctcggggtc gagccccgga
    12661 tccggtggct ctcccacccg atcgccgcgc agtgggcgat catcctggcc gacgtctggc
    12721 agtggacctc cctcaccttc ctgatcttcc tgtcgggctt cgccgccctg ccccagccgc
    12781 tcgtgaacgc cgcccgcgtc atgggcgcca gccgctggca gatcttccgg cgcgtgcagc
    12841 tgcccctgct caagcccgtc atcgtcatcg ccgtcgtcat ccgcggcatg gaggcgctca
    12901 agatcttcga ccagtcggtc ctgctcacct tcggcgggcc cggcaactcc acccagacgg
    12961 tggccttcta cctctggcgc caggtgtggc agttcaacaa gttcagcttc ggggcggcgg
    13021 cctcgatgct gttgctcgtg atgttcgccg tcctgatcta cgccggcatc tacctgctcg
    13081 tgcggcagcg cagcaccgtc ttgaaggagg gccgcgcatg agcgccggcg cccggccgcg
    13141 gccgcatgcc gtccgtgcgc gcggccggct cacgcggcgg ctcttcccct acgcccgcca
    13201 cgcggccctg ctggcgtggt cgagcttcgt gctgttcccg atcttctgga tggtgtccac
    13261 gtccttcaag gagtcgtggg agtgggtcgc gtggccgccg cactggctcc cccgcgagcc
    13321 cacactccag agctaccgcc aaatcttcac gttcagcgag atggggcgcg aactggcccg
    13381 cggggccacc gatcaggtct tcgccatctg gggggcggtc gccgactcgc tcgtcatctg
    13441 cacattgagc tcgctggcct cgcttctcct gggcaccttc ctcgcctact cggtgtcgcg
    13501 gttcggcgtg ggcggcaaga acttccgcta cgcgatcctg accatccgca tggtgccgcc
    13561 gatcgtgatc gccatcccga tcctgatcta ctacgccatc atgatccccc acacgacgac
    13621 cgtgctcttc ggcgcccggg cgagcctcat cgacacctac ctgggactcg gcctcctcta
    13681 cacggccacg accctgcctt tcgtgatctg gatgatgctg agcttcatcg acgaggtccc
    13741 ctacacgctc gagcacgcgg cgcgcctgat gggcgccggg cgcctcgcca cgatccgccg
    13801 ggtggtgctg ccgctggtgg cctcgggcat ggtggtgaca ttcctcttca tcttcatcct
    13861 gaactggagc gagttcctgc tcgccctcac gctcacgcat ccgaaggtca ccacgctgcc
    13921 cgtgctcctc aacaagtttc agagcgccgt ggaggggcgt ctctacggac cccaggccgc
    13981 catcggcacg gtcatcaccg tccccgtcgt cgtgctgggg atcctgatcc agaagcacct
    14041 cgtcaagggg ttcagcttcg ggatgatccg caagtgagcc gcatcctcct ccggagcctg
    14101 gtcaagacgt tcggcggcgt gaccgccgtg gccggcgtga gcctggaggt ccgcgacggc
    14161 gagttcgtgg tgttcgtggg cccgtcgggc tgcggcaaga ccaccaccct gaacatgatc
    14221 tcggggctcg agaccccgac gagcggcgag gtcgtcatcg gcgacctggt cgtgaacgac
    14281 gtcgagcccg gcgagcgcgg cctgggcatg gtcttccagg acctggcgct gttcccgcac
    14341 atgaccgtgt tcgagaacat cgccttcggc ctccgggtca agcgcgtcgc cgaggccgag
    14401 gtgcgccagc gcgtccagga ggccgccgcg acgatgcgca tcgccgcgct gctggccaag
    14461 aagccggcgc agtgctcggg cggcgaggcg cagcgggtgg cgctggcgcg caccatcgtc
    14521 accaagccgt ccgtgttcct catggacgag ccgctctcga gcctcgacgc caagctccgc
    14581 gtggagatgc gcaccgagct caagcgcctg cacggggcgc tcgaggcgac gttcgtctac
    14641 gtgacccacg accaggccga ggccatgacc atggccgacc gcatcgtggt gatggacc
//
LOCUS       DATIAZ010000199         3436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1104776, whole genome shotgun sequence.
ACCESSION   DATIAZ010000199 DATIAZ010000000
VERSION     DATIAZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104776"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1253
                     /locus_tag="VLF19_07100"
     CDS             join(187..672,675..1253)
                     /locus_tag="VLF19_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611714.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HSE93056.1"
                     /translation="MGAALPIRDDLTPAALRRHARHEPNRRAALRMLAVANALEGMSR
                     AEAARLAGMERQALRDAVVRYNAEGIAGLKDRPKPGRPERLSEAEQAALAARVFRGPD
                     PERDGVSAWTRADLCRWLVERFGKAFHPSSLSRVLKRLELSRQKTRPVHPETDPKAQE
                     RFKKGLRDALKAAAEAHPGKRIALWFMDEPRVGQKGRLCHRWWRKGHRPPGRRDRRFE
                     WAYIFAAVEPATGADVALVLPEATTVTMNLFLAEFAAGLPEEVHAVLVLDGAGWHGAR
                     ALTVPPNVTLVPLPPYSPELNPVERVWLYLRERFLSLRVFADYRAILDACCAAWNQLV
                     AEPGRLRSLCDQPWIRNVTS"
     gene            1490..2989
                     /gene="istA"
                     /locus_tag="VLF19_07105"
     CDS             1490..2989
                     /gene="istA"
                     /locus_tag="VLF19_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSE93057.1"
                     /translation="MFSVELYARVRHACHVEGLSQREAARRFGIHRNTVRKMLTFSVP
                     PGYRRSKPPLRPKLDGFTAAIDAILEADRTAPAKQRHTAKRIHDRLRAEHGFAGGYTI
                     VKDYVREQRCRAREVFVPLAHPPGHAQVDFGEAVAVIGGERRKIHFFCLDLPHSDAGF
                     VKAYPAERIEAFLDGHNAAFAFLGGVPQSILYDNTQLAVARILGDGRRQRTRSFAELQ
                     SHYLFLDRFGRPGKGNDKGKVEGLVGYARRNFLVPIPAFASFAALNAHLEQRCLDRLG
                     DQVRGQSESIGERLERDLAALQPLPPAPYDACDQRPGRVSALSLVRYDRNDYSVPTAY
                     GHRPVLVKGYVEQVVIACGAEIIARHPRSYAREDFVFDPLHYLALLEQKLGALDQAAP
                     LQGWGLPEAFATLRRLLEARMGKHGKRAYVQVLRLLETFRLEEVHAAVREALRLGAIG
                     FDAVKHLVLCRIERRPPKLDLAAYPYLPRVTVATTSAKAYMSLLGGPAA"
     gene            2986..>3436
                     /locus_tag="VLF19_07110"
     CDS             2986..>3436
                     /locus_tag="VLF19_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE93058.1"
                     /translation="MSKEVPQVLLAHHLKALKLPSFLREHDKLARQCATEGVDHTGYL
                     LRLAELELLDRERRLVERRIRQARFPAVKSLDSFDFTALPALNKLLVLELARGEYIEW
                     RENVIALGNSGTGKTHIALGLGLAACQKGLSVGFTTAAALVHELLEAR"
BASE COUNT          509 a         1205 c         1184 g          538 t
ORIGIN      
        1 gctgccgcgc ttcctgcata gtgccgcccg caccgacccg ctgccctacc ggctcctcac
       61 ggccggatga gaatgcgggc tgatcaggaa acatcttata ccggcgcaca gatgaactga
      121 ctttccgggc tcggcgtggc gtgctctcct ggtcggcacg aacgacggcc gacgagggag
      181 ggggcgatgg gcgcggcgct gccgatccgg gatgacctga cgccggcggc gctgcgccgg
      241 cacgcgcggc acgaaccgaa ccggcgggca gcgctgcgga tgctggcggt cgccaacgcc
      301 ctggagggga tgagccgggc cgaggctgcc aggctcgccg ggatggaacg ccaggcgctg
      361 cgggatgcgg tggtacgcta caacgcggaa gggatcgcgg gcctcaagga ccggcccaag
      421 cccggccggc ccgagcggct gagcgaggcg gagcaggcgg ccttggcggc gcgggtgttc
      481 cgcggccccg atccggagcg ggacggcgtc tcggcttgga cgcgtgccga tctgtgccgc
      541 tggctcgtgg agcgctttgg caaggccttc cacccgtcca gcctgtcgcg ggtgctcaag
      601 cggctcgagc tgtcgcggca gaagacccgg cccgtccacc ctgagacgga cccgaaggcc
      661 caggagcgct tccgaaaaag gggctgcgcg acgccctgaa ggcggcggcc gaggcgcatc
      721 ccggcaagcg gatcgcactc tggttcatgg acgagccccg agtgggccag aagggccggc
      781 tgtgccaccg ctggtggcgc aagggccacc ggccgccggg gcgccgcgac cggcgctttg
      841 agtgggccta catcttcgcc gccgtcgagc ccgcgaccgg cgcggatgtc gccttggtcc
      901 tgccggaagc gaccactgta accatgaacc tgtttttggc cgagttcgcc gccggcctgc
      961 ccgaggaggt gcatgcagtg ctggtcctcg acggtgccgg ctggcacggc gctcgcgcgc
     1021 tgaccgtccc gcccaacgtc accctcgtcc cgctgccgcc ctacagccca gaactcaacc
     1081 cggtggaacg ggtctggctt tacttgcgcg agcgctttct ctccctgcgg gtctttgccg
     1141 actaccgcgc catcctcgat gcctgctgcg ctgcctggaa ccagctcgtt gccgagccgg
     1201 gccgtctgcg ctccctctgc gaccagccgt ggatcaggaa cgtcacttca taggctcggc
     1261 ggtataatac agctcgatct cgtgcccatg gctcttgacc caggccgtca ccttgccggc
     1321 cttgtggacc ttcagattgt cgacgatcag aacggccttc cgtcctctaa gcggcggtga
     1381 gaaagtgtcc ctctgaggcg gccggatagc ccggtcgcgg cggtacgaaa ctgtaccggc
     1441 cgacagcctt cttggtgctc gtcagcaacg agcgggagaa ggcggaggga tgttctcggt
     1501 ggagctctat gcgcgggttc gtcatgcctg ccacgtcgaa gggctgagcc agcgcgaggc
     1561 ggcgcggcgg ttcggcatcc accgcaacac cgtgcgcaag atgctgacct tctcggtgcc
     1621 gccgggctac cggcgcagca agccgccctt gcggcccaag ctcgacggct tcaccgcggc
     1681 catcgacgcg atcctggagg cggatcgcac cgccccggcc aagcagcggc acaccgccaa
     1741 gcggatccat gaccggctgc gggccgagca tggcttcgcc ggcggctaca cgatcgtcaa
     1801 ggactatgtc cgcgagcagc gctgccgggc acgcgaggtg ttcgtgccgc tggcccaccc
     1861 gcctggccac gcccaagtcg acttcggcga ggcggtggcg gtgatcggcg gcgagcgacg
     1921 gaagatccat ttcttctgcc tcgacctgcc gcactcggat gccggcttcg tcaaggctta
     1981 tcctgccgag cggatcgagg ccttcctcga cgggcacaac gccgcgttcg ccttcttggg
     2041 cggcgtgccg cagtcgatcc tctacgacaa cacccagctg gcggtggccc gcatcctcgg
     2101 cgacggccgg cgccagagga cgcgcagctt tgccgagctg caatcgcact acctgttcct
     2161 cgatcgcttc ggccggcccg gcaagggcaa cgacaagggc aaagtcgagg gcttggtcgg
     2221 ctacgcccgg cgcaacttcc tggtgccgat cccagccttc gccagctttg ccgccctgaa
     2281 cgcccacctt gagcagcgtt gcctcgatcg gctgggcgac caggttcgcg gccagagcga
     2341 gagcatcggc gagcggctgg agcgcgacct cgccgccctg cagccgctgc cacccgcacc
     2401 ctacgatgct tgtgatcaaa ggccggggcg ggtctccgcg ctgtcgttgg tgcgctacga
     2461 ccgcaacgac tactcggtgc cgaccgccta cggccaccgc ccggtgctgg tgaagggcta
     2521 cgtcgaacag gtggtgatcg cctgcggcgc cgagatcatc gcccggcatc cgcgctccta
     2581 cgcgcgcgag gatttcgtct tcgacccgct gcactatctg gcgctgctgg agcagaagct
     2641 tggcgccctt gatcaggccg cacccttgca gggctggggc ctgcccgaag cgttcgcgac
     2701 gctccgccgc ctgctcgagg cccgcatggg caagcatggc aagcgggcct acgtccaggt
     2761 gctgcggctg ctggagacct tccgcctcga ggaggtccat gccgccgtgc gcgaggcgct
     2821 ccggctcggc gccatcggct ttgatgcggt caagcacctg gtcttgtgcc ggatcgagcg
     2881 tcggccgccc aagctcgacc tggcggccta tccctacctg ccccgggtca cggtcgcgac
     2941 cacctcggcc aaggcgtaca tgagcctgct cggcgggccg gcggcatgag caaggaggtg
     3001 ccgcaggtcc tgctcgcgca ccacctcaag gcgctcaagc tgccgagctt cctgcgcgag
     3061 cacgacaagc tcgcccggca gtgcgcgacg gaaggcgtgg accacacggg ctatcttttg
     3121 cgcctggccg agctggagct gctcgaccgg gagcggcggc tggtcgagcg gcgcatccgc
     3181 caagcccgct tcccggcggt gaagagcctg gacagcttcg acttcaccgc actcccggcc
     3241 ttgaacaagc tgctggtgct ggagctcgcc cgcggggagt atatcgagtg gcgggagaac
     3301 gtgatcgcgc tgggcaacag cggcaccggc aagacccaca tcgcgcttgg gctcgggctg
     3361 gccgcctgcc aaaagggcct ctcggtcggg ttcaccaccg cggccgcctt ggtccacgag
     3421 ctgttggagg cccggg
//
LOCUS       DATIAZ010000200         4766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1763757, whole genome shotgun sequence.
ACCESSION   DATIAZ010000200 DATIAZ010000000
VERSION     DATIAZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763757"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VLF19_07115"
     CDS             <1..319
                     /locus_tag="VLF19_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HSE93059.1"
                     /translation="ASEQGALPELRRVIVAYGNQIAMEPTLEQSLARIFGGRPSAPAM
                     VTPTPPSAPRDAPAGGHTLAQQAWEIWQRAQDALRRGDWAQYGAEQKRLEEALRALTG
                     AAR"
     gene            387..1439
                     /locus_tag="VLF19_07120"
     CDS             387..1439
                     /locus_tag="VLF19_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93060.1"
                     /translation="MKRCALLCLAVVACGLAAAPPAAGGAADRLDRFRTLATSRLTLA
                     DVGDPERAGEAYREVLALLDDEIVESLVSGGPFASAAFLQDRLDGFSEAWGGASLRLT
                     RVGPFTVGAFQLGESPGGSAVHVYGTPRGGEPQLLSAFAREGRPTVHVLSPASGGAAR
                     FAVVWEGVPSGRGTRAVRVDLVRQVGDDVTVTWSTAQVYPDGLVARAWRLRGDEMYVR
                     YELRYPGWIPGCEGQTEQEDVLRLGADGTGFTRVTRRQHDAWHHALHRSVERLFEALS
                     TGDRAALAAVVPDAALRQRLPAGLAREDACDAPDGPGPAAVSVAASAGEGGPWTLTFR
                     RSGGEWRLDAVAPVLP"
     gene            1454..4105
                     /locus_tag="VLF19_07125"
     CDS             1454..4105
                     /locus_tag="VLF19_07125"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSE93061.1"
                     /translation="MSEASAVIADRYEPSAVEARWYPEWERRGFFHADPASPKKPYSI
                     VIPPPNVTGSLHMGHALDNTLQDVLIRMKRMDGFEALWVPGTDHAGIATQYVVERQLV
                     AEGTTRDDLGRAAFLERVWRWKEASGGAIIRQLKRLGASCDWSRERFTMDPGLSRAVR
                     EVFVRLYDEGLIYRDDYIVNWCPRCQTVLADLEVEREERDAEFVYIKYGPLTLGTVRP
                     ETKLGDTGLAVHPNDERYTPYVGQTLAIESVDGPITIKVVADEAVDPEFGTGVIKVTP
                     GHDPVDFEIGRRHHLPIRTVIGFDAKMTAAAGKYAGLDRFECRKRIVEDLRQRGLIER
                     IEPYRHAVGLCYRCKTIVEPLVSKQWYVNVKPLAAAAITAVRTGRIRIVPRGWARTYY
                     HWMENIRPWCISRQLWWGHRIPAWHCEREGSVHVSRADLQACPACGGPVRQDDDVLDT
                     WFSSGLWPFSTLGWPDDTPELRKFYPTSVLVTGYDILFFWVARMAMLGVHVMGEVPFR
                     DVYIHTLVRDAEGQKMSKTRGNVVDPLLLMDKYGTDAFRFTLAGLSVPGVRDVRIAED
                     RVEAGRNFANKLWNAARLVLTNLDGYEPRRAARVPPGLPERWITSRLQAAVGAVREHL
                     RRYRFNDATSAIYQFVWDEYCDWYLEVAKTALYRPESPERRLSAQHALVTVLETTLRL
                     LHPFMPFITEELWQRLPSAVRRGESVMVAKYPRVARKAVDRQAEEEFGVVMSLIGGIR
                     NIRGEMRIPPSLTLTTIVKPGAPHERLAIDTSPLIQTLGRTALTVDRVATRPPNSAIA
                     VVGPTEIYVDLAGVVDLAAERARLEKEIKRAADTVAFLETKLSRPEFVERAPATVVDK
                     ERERLDAERQLRAKLEASLSWIAEGAR"
     gene            4126..>4766
                     /locus_tag="VLF19_07130"
     CDS             4126..>4766
                     /locus_tag="VLF19_07130"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSE93062.1"
                     /translation="MSAPHVVRVGTVVSTQDVAFDLAARGAADRTVVLADHQTAGRGR
                     QGRTWHDAPGASLLVSILVRPALDARSLPWLSYVAAIAVAEALQEATGLAPRLKWPND
                     VLVDDRKVAGILLQTRLSASTRGDGAATASNGPSTQRAGLAAALTVIGVGINLEPGSV
                     TPELADRATSVREAGGRPVDREALLGAVVAALEVWRARLEREGPEPIRRRWLAL"
BASE COUNT          649 a         1746 c         1716 g          655 t
ORIGIN      
        1 cgcctcggag cagggagccc tgccggagtt gcggcgcgtg atcgtcgcct acggcaacca
       61 gatcgcgatg gagccgacgc ttgagcagtc gctggcgcgc atcttcggcg gccgaccgtc
      121 ggcacccgcg atggtcacgc ccaccccgcc gagcgccccc cgcgacgccc ccgccggtgg
      181 acacacgctc gcccagcagg cctgggagat ctggcagcgg gcccaggacg ccctgcggcg
      241 cggcgactgg gcccagtacg gagccgagca gaaacgcctc gaggaggcgc tgcgcgcgct
      301 caccggagca gcacgctagt cgcggtcgcc cgcgcctcga gcccggcgac ggcgggacct
      361 ccgggggtct gttgtaccat agccctgtga agcgctgtgc gctgctgtgt ctcgccgtgg
      421 tggcgtgcgg cctcgcggcg gcgccgccgg ccgccggagg tgccgccgac cggctcgacc
      481 gcttccgcac gctggccacc tcacggctca ccctggccga cgtcggtgac cccgagcgcg
      541 ccggcgaggc ctatcgcgag gtgctcgcgc tgctcgacga cgagatcgtc gagagcctcg
      601 tcagcggggg gccgttcgcc tcggccgcct tcctgcaaga ccgcctcgac ggcttctcgg
      661 aggcgtgggg cggcgcctcc ctccgtctca cgcgcgtcgg gcccttcacc gtcggcgcct
      721 tccagctcgg tgagagcccc ggcgggagcg cggtgcacgt gtacgggacc ccgcggggag
      781 gtgagcctca gctcctctcc gcgttcgcgc gcgagggacg gccgaccgtg cacgtgctct
      841 cgcccgcgtc gggcggcgcc gcgcggttcg ccgtcgtgtg ggaaggcgtg ccctcggggc
      901 gcggcacgcg ggccgtgcgg gtcgacctcg tccgccaggt cggcgacgac gtgaccgtga
      961 cctggagcac cgcgcaggtg tatcccgacg gcctcgtggc gcgcgcctgg cggttgcgag
     1021 gcgacgagat gtacgtccgc tacgagctcc gctaccccgg atggatcccc ggctgtgagg
     1081 gacagacgga gcaggaggac gtgctgcgcc tcggcgccga cggcacgggc ttcacgcgcg
     1141 tgacccgacg gcagcacgac gcctggcacc acgcgctgca tcgctcggtc gagcgcctct
     1201 tcgaagcgct gtccaccggc gaccgcgcgg ccctggccgc ggtggtcccc gatgccgccc
     1261 tccgccagcg gttgccggcg ggcctggcgc gtgaggacgc gtgcgacgcg cccgacgggc
     1321 cgggcccggc ggcggtgtcc gtcgccgcct cggcgggcga aggcgggccg tggacactga
     1381 cgttccggcg gagcggcggt gagtggaggc tcgacgccgt ggcgccggtg cttccctgac
     1441 gagctccccg ccgatgagcg aggcctccgc cgtcatcgcc gaccgttacg agccgtccgc
     1501 ggtcgaggcg cgctggtacc ccgagtggga gcggcgtggg ttcttccacg ccgatccggc
     1561 ctcgccgaag aagccctact cgatcgtgat cccgccgccg aacgtgacgg gctcgctcca
     1621 catgggccat gcgctcgaca acacgctgca ggacgtcctc atccgcatga agcgcatgga
     1681 cggcttcgag gcgctctggg tgccgggcac cgaccacgcc ggcatcgcga cccagtacgt
     1741 cgtggagcgg cagctcgtcg ccgagggcac gaccagggac gacctgggcc gcgccgcctt
     1801 cctcgagcgc gtctggagat ggaaggaggc gtcgggcggc gccatcatcc gccagctcaa
     1861 gcggctcggg gcctcgtgcg actggtcgcg cgagcgcttc acgatggacc cggggctctc
     1921 gcgcgcggtg cgcgaggtct tcgtgcgcct gtacgacgag ggcctcatct accgcgacga
     1981 ctacatcgtg aactggtgcc cgcgctgcca gaccgtgctc gcggacctgg aggtcgagcg
     2041 cgaggagcgc gacgccgaat tcgtgtacat caagtacggt ccgctcacgc tgggcacggt
     2101 gcggcccgag acgaagctcg gggacaccgg cctcgccgtg catccgaacg acgagcgcta
     2161 cacgccgtac gtcgggcaga ccctcgcgat cgagtcggtg gacgggccga tcacgatcaa
     2221 ggtcgttgcc gacgaggccg tcgacccgga gttcggcacg ggcgtgatca aggtcacgcc
     2281 ggggcacgac ccggtggact tcgaaatcgg gcgccgccac cacctgccga tccggaccgt
     2341 catcggcttc gacgcgaaga tgacggcggc cgcgggcaag tacgccgggc tcgaccgctt
     2401 cgagtgtcgc aagcgcatcg tcgaggatct gcggcagcgg ggcctcatcg agcgcatcga
     2461 gccctaccgc cacgccgtcg gcctctgcta tcgctgcaag acgatcgtgg agccgctcgt
     2521 ctccaagcag tggtacgtca acgtcaagcc gctggccgcc gcggccatca ccgccgtgcg
     2581 cacgggccgg atcaggatcg tgccgcgcgg ctgggccagg acctactacc actggatgga
     2641 aaacatccgg ccctggtgca tctcgcggca gctctggtgg ggccaccgga tcccggcctg
     2701 gcactgcgag cgggagggca gcgtgcacgt ctcgcgcgcc gatctgcagg cgtgccccgc
     2761 ctgcggcggc ccggttcgcc aggacgacga cgtgctggac acgtggttct cctcggggct
     2821 gtggccgttc tcgacgctcg gctggcccga cgacacgccg gagctccgga agttctaccc
     2881 cacgtccgtg ctggtcaccg ggtacgacat cctgttcttc tgggtcgcgc gcatggccat
     2941 gctcggcgtg cacgtcatgg gcgaggtgcc cttccgcgac gtctacatcc acacgctcgt
     3001 ccgcgacgcc gagggccaga agatgtccaa gacgcggggg aacgtggtcg atccgctcct
     3061 gctcatggac aagtacggca cggacgcctt ccggttcacg ctcgccggcc tctcggtgcc
     3121 cggggtccgt gacgtccgga tcgccgagga ccgcgtcgag gcgggccgca acttcgccaa
     3181 caagctgtgg aacgccgcgc gtctcgtcct gacgaacctc gacggctacg aacctcgccg
     3241 cgcggcgcgc gtgccccccg gcctgcccga gcggtggatc accagccgcc tgcaggccgc
     3301 cgtcggcgcc gtccgcgagc acctccggcg ctaccgcttc aacgacgcga cctcggcgat
     3361 ctaccagttc gtctgggacg agtactgcga ctggtacctg gaggtcgcca agaccgccct
     3421 gtaccgcccc gagagcccgg agcggcgcct gagcgcccag cacgctctcg tcaccgtcct
     3481 cgagacgacg ctgcggctgc tccacccgtt catgcccttc atcaccgagg agctgtggca
     3541 gcggctcccc tcggccgtcc ggcgcggcga gtcggtcatg gtggccaagt acccgagggt
     3601 ggcgcgcaag gccgtcgatc ggcaggccga ggaagaattt ggtgtcgtga tgtccctgat
     3661 cggcggcatc cggaacatcc gcggggagat gagaatcccc ccgagcctga cgctcacgac
     3721 gatcgtcaag ccaggggcgc cgcacgagcg cctggccatc gacacgtcgc ctctcattca
     3781 gacgctcggg cgaacggcgc tcaccgtcga ccgggtcgcg acgcggcccc cgaactctgc
     3841 catcgccgtc gtggggccga cggagatcta cgtggacctc gccggggtcg tcgacctggc
     3901 cgccgagcgg gcccgcctcg agaaggagat caagcgcgct gccgacacgg tcgccttcct
     3961 cgagacgaag ctgtcccgcc cggagttcgt cgagcgggcc ccggcgaccg tggtggacaa
     4021 ggagcgcgag cgcctcgacg ccgagcgcca gctcagggcg aagctggagg cgagcctgtc
     4081 ctggatcgcc gaaggcgccc gttgagccgg gcccgggaca cggtcgtgtc cgcgccgcac
     4141 gtcgtccgtg tggggaccgt cgtctccacc caggacgtcg ccttcgatct cgccgcccgc
     4201 ggcgccgcag accgtacggt cgtgctcgcc gaccaccaga cggcgggccg cgggcgccag
     4261 ggacggacgt ggcacgacgc acccggcgcg agcctgctgg tgtcgatcct cgtccggccc
     4321 gcgctggatg cgagatccct gccgtggctg tcctacgtgg ccgccatcgc ggtcgcggag
     4381 gctctccagg aggccaccgg gctcgccccg aggctcaagt ggcccaacga cgtgctcgtc
     4441 gacgaccgca aggtcgccgg catcctgctc cagaccaggc tgagcgcgtc gacccggggt
     4501 gacggagccg cgaccgcctc gaacggccca tccacccagc gagccgggct cgcggcagcc
     4561 ctcacggtca tcggcgtcgg gatcaacctc gagccgggga gcgtgacgcc ggagctggcc
     4621 gaccgcgcga cgagcgtgcg cgaggccggc ggccggcccg tggaccgcga ggcgctgctc
     4681 ggcgcggtcg tggcggcgct ggaggtttgg cgggcccggc tcgagcggga ggggcccgag
     4741 ccgatccggc ggcgctggct cgccct
//
LOCUS       DATIAZ010000201        12476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1763810, whole genome shotgun sequence.
ACCESSION   DATIAZ010000201 DATIAZ010000000
VERSION     DATIAZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12476
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763810"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VLF19_07135"
     CDS             complement(<1..458)
                     /locus_tag="VLF19_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93063.1"
                     /translation="MTLSPRHFLASLALLFALVSAHAAWTTHRSQVELRVQMEQRGLA
                     VADAVEAGSRNAIRSNALMEETIAQRLLDNARLVDALLARPIGIVDLARVTEQNGLSR
                     VDLLDRDGRPWAPPEPSHGMMRMMMAPPAGPWTPGGDRAMMHYMWGRRWAA"
     gene            complement(529..939)
                     /locus_tag="VLF19_07140"
     CDS             complement(529..939)
                     /locus_tag="VLF19_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93064.1"
                     /translation="MKKVVSVMFAVVMVGMLAGVALAHEPGWGPMMGGRSPMMAGRSP
                     MAGGPEACPMWGAAGTEAGTEAVTEDKARELANEYVTKYFKGFTIERVLPFIGRSRTM
                     YQVELRGPKGETRYLHVTPRGDVRPFGPLAATTD"
     gene            complement(1122..1481)
                     /locus_tag="VLF19_07145"
     CDS             complement(1122..1481)
                     /locus_tag="VLF19_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93065.1"
                     /translation="MEWVSLFVAGLVLVLIAAPVVLLLGTFLVLLPLSLLAPPAPALA
                     RASFTCPVTRRPVTAAFVTRAGASRPADVVACSMFTDGVRCAKGCLGSAYTSWTPSSA
                     VARYALLADGEALREAA"
     gene            complement(1679..3958)
                     /locus_tag="VLF19_07150"
     CDS             complement(1679..3958)
                     /locus_tag="VLF19_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HSE93066.1"
                     /translation="MRIQRAAGWLIGGVGLVVGTLAWAPSASGQLSLGGFNLEGDVEA
                     GVRAFIERPSPSRRGKFEEYRDIDQGPFLGEFQLRFFRPDESYSVTLGGSKWGQDDQE
                     YVLRAGRLGLWEFGFAWDQTPHLLSTTARTRAVEVSPGEWRLPARPTATSISAAEGAL
                     YNAAPELDEVGVRWDTARISLLVTPRPDLDFRADYARIHKDGTQPFGMAMGSPGNDFI
                     EVLEPIDHTIHDFRLRGTIARDTWQLQAGYIFSRFENSNDRVIADNPLRSVFAAFAPS
                     ATGGSSSPAFGRSSLAPDNMAHTIQISGGVDLPWRTRLSSGFSYSLRLQDDDFLPHTI
                     NPNIPTALLTLPQESLDGIVGITAINLSLVNRAIPRTTLSLRYRFFDHSEMTDEVVFP
                     GHAVNDQALSNEERRTSAFDHSRHNLDLDAKYRPISGLATTLGGGWEHWKRDEEHREV
                     PKSDEYYLKAALDWTPLEWLLTRLTYKPSWRRIDEYNTFAHIAHTVLEEELSPTEQAQ
                     SQSLLLRKFDQGERDRQQLDLTLQFTPLDTVTLTANGMWRNDDYLGGQFGLRDATTWS
                     AGFDVSWSPTERVALFAGYVYEYIYQKFASRNRDVATVAGQSIVSDFEDWNWLSVNLD
                     RVQTAHIGAKVGIIPNKLDWTVQAGWSYAFGEVDTRNETAPLPHPPGFPNPANAVARR
                     QPAFEDMLVRLDTAFRYWVTRQWAVSFGYAFETFKGSDWRTDGLNPFVPGSNSIRLGN
                     DSRNYTAHIVGATVRYVFK"
     gene            complement(3968..4936)
                     /locus_tag="VLF19_07155"
     CDS             complement(3968..4936)
                     /locus_tag="VLF19_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HSE93067.1"
                     /translation="MRDRHGRRGALHHLFLPLMVALLLTGCFKAADTPRPVAQPPAPP
                     SAADYVGAETCKACHEESYHQFAATKMGKLFLRHPRSAQEKNACESCHGPGKAHAESP
                     GRGKDAKLITFAKDDPTPVEIRNKVCLDCHSKGQRVFWSGSAHDARDVSCTGCHRVMT
                     DHSSKAQLAKATEIETCGSCHLQRRAQQMRSSHMPLREGKMTCTSCHNPHGTISQALL
                     REPSINDSCFTCHTEKRGPFLWEHAPVVESCVNCHDPHGSNHERMLKTAKPRLCQQCH
                     VETRHPTRPYGREAPANLRFVLGRSCANCHVNIHGSNHPSGFAFTR"
     gene            complement(5336..5944)
                     /locus_tag="VLF19_07160"
     CDS             complement(5336..5944)
                     /locus_tag="VLF19_07160"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HSE93068.1"
                     /translation="MKASTKREAAPRDWPVAAVAAAGLAISAHLAVAKLAGGQALLCE
                     AGGGCDIVQLSRYAIFLGLPTAAWGAALYAVVTALALVGLAVRRWLAVFALAAAALAF
                     SSYLTYLQLFVVRAICPWCVADAVVAVALVVTVLLRRPPARRRQTGPGRLALVGGGAA
                     VATVVFAAGVFVADAPSEHREYREALARHLSASGAIFYGAYW"
     gene            6010..6300
                     /locus_tag="VLF19_07165"
     CDS             6010..6300
                     /locus_tag="VLF19_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93069.1"
                     /translation="MNRVAGWSVVAAVMAVSLGAGPAMAEPAMLKQAKDAGLPAQNCQ
                     YCHTTKIPKKETFKPEELTDRGKWLRAQKEKRKAPKAEAAWLKEYPGGPAQK"
     gene            complement(6311..7672)
                     /locus_tag="VLF19_07170"
     CDS             complement(6311..7672)
                     /locus_tag="VLF19_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSE93070.1"
                     /translation="MKRTVTWLALAAALVGGAAAPAQTRSPRETAPSPAPPPSGPARP
                     PSAGPGGATGHGDSELDCRNCHIGKHRGVAQMYVGVGGRGTPMIPSHMFQVRVECVAC
                     HGTPASAAGAAAITGQTFRPSERACLGCHGEKYRGMLAQWGTTLEHMRQAIAPKLAAA
                     RAALGGDPKSPKLARARKLVEDAEFNVRFVGLAKGVHNVFYAADLLKLANGWLDEAIA
                     ASGAPAPKTDDQLVRGGYCAVLCHQVAGVKVLATATFQQRRFPHARHVTDFGATCTSC
                     HSAETHKAVTATPATCRGCHHGPQNDRCETCHSAQAAFYRGTAHTPDAKIAPNLMADA
                     VPCTGCHAFSGTHSREAVGKACLNCHDAPYVALLREWTTGYRNDVRRAAQAVRTAEAA
                     LAEAKRAGRAVREADALLRQARSALALVRGAGVAHNPLAADALLATARAKAEQARARV
                     SAR"
     gene            complement(7669..8616)
                     /locus_tag="VLF19_07175"
     CDS             complement(7669..8616)
                     /locus_tag="VLF19_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HSE93071.1"
                     /translation="MSDDAPGGGRTPRRRWRGPVILLAAAIVLAAGVAAGAGLWHVSA
                     SPRFCNSCHIMRPYVDAWKASSHAAVACVECHYPPGLRDTLRVKFQAVTQVAKWATGT
                     YSSKPFAEVEDASCLRSGCHARTDLERKGELTSRRGIRFDHARHLTAAMGRELRCTSC
                     HAQMVVDRHFEVTQSACFTCHFKARRNGRELIPVAGCTGCHGVPRGDIVVGSVRFNHD
                     EVAQRGVACQSCHLSVVEGRGDAPRERCMGCHNQREVIAKYDDARLVHAAHVSARSIE
                     CTRCHSEIKHRLPPRIGPPTADAAPPALAVLATGPGGGR"
     gene            complement(8641..9033)
                     /locus_tag="VLF19_07180"
     CDS             complement(8641..9033)
                     /locus_tag="VLF19_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HSE93072.1"
                     /translation="MLAQRPGRPLTPTRPGALVVAVLATGFLLAGPGLDRGPGFAQGK
                     APPDFAIKPGEGSPGPVTFSHAAHLGKVGKCTRCHMRDLKMKRGASGPITLEAKQQGR
                     FCGACHDGKTMMAGAVVFSIDECDRCHK"
     gene            complement(9003..9671)
                     /locus_tag="VLF19_07185"
     CDS             complement(9003..9671)
                     /locus_tag="VLF19_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSE93073.1"
                     /translation="MTGAGPGRRRARSLAAVAWAVALVAPALVPVAPVLAPPTASAQP
                     TGDAVRGEQLFVAKACARCHRTAASGAMPALQTLRRPQGSWELAGRMWNHAPAMFMLL
                     TQEGLAWPQITVPEMADLMAYLSAHPARDPAPDLVRGQLTLIAKGCLKCHAFRREGAR
                     LGPDLADQRPAYGAPAAWAAAMWTHTPRMAAVALQRGFLYPRFTGDELINLVGFLRSG
                     RGAP"
     gene            complement(9683..10783)
                     /locus_tag="VLF19_07190"
     CDS             complement(9683..10783)
                     /locus_tag="VLF19_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSE93074.1"
                     /translation="MAPAEPRARGPVWRWLDERVGLSEIQRLAKKKEVPDHRHSLWYY
                     FGGMALFLFVVQVGTGILLLFYYRPSAEEAYESIQFLMAEVQFGWLVRSIHSWAANLM
                     VFVVFVHLFSVMLLRAYRPPREVTWISGVVLLALALGFGFTGYLLPWNELAYFATKVG
                     TEIPGVLPVVGGFLRRLLRGGDEVTGATLTRFYGIHVAVLPAIAAALLGLHLFLVQKH
                     GMSVPPSVERAGGPRRMMPFFPSFFLRDLVGWLAALAALATLAAFWPAELGRKADPFA
                     AAPAGIKPEWYFMFMFQTLKYLPGHILGIEGELIGVLGFGLGGLFLLLIPFFDRGAGR
                     GAPNRVLPAIGLAIIVYMLALTYLGYTASPTK"
     gene            complement(10792..11244)
                     /locus_tag="VLF19_07195"
     CDS             complement(10792..11244)
                     /locus_tag="VLF19_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSE93075.1"
                     /translation="MADETLDLGASRRAVNWFLATSLGAFLVAVLYPVGRYLVPPEAG
                     ESTAGTVTLPLKAADVKPNTGQIFKFGSRPGILVRTPAGELRAFSAVCTHLNCTVQFR
                     ADLGHIWCACHNGHFDLNGKNIEGPPPRPLDTFVVNVRGDQIVVSKST"
     gene            complement(11234..>12476)
                     /locus_tag="VLF19_07200"
     CDS             complement(11234..>12476)
                     /locus_tag="VLF19_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93076.1"
                     /translation="LVPARAGAQAPAADQCTACHLEIGDDRLVNPAKAYAEDVHRSRG
                     LGCVACHGGDAKAAGMEAMDPAKGYIGKPARAQMAQVCGRCHSDAQFMRQYNPALRVD
                     QVAEYLTSVHGRRLRQGDQKVAVCASCHPAHSIRPASDPKSSVHPMKVAVTCGRCHAD
                     AAYMKGYRIPTDQLEKYRTSVHWQAMSAKGDLSAPTCNDCHGNHGAAPPGVSWVGNVC
                     GQCHAVMADLFRASAHAKVLVDMGSPGCATCHGNHAIARASDDLLGVGKDAACASCHG
                     ADDAGGRVASAMRGRIDALHSAVDGARSVLTHAEQSGMEVSQALFDLNGAADALVKAR
                     AAVHAFTASAVDKEVAPGLTIAEKALQRGLRAMDELRFRRAGLGVSLVVILALIAAVV
                     LKIRQIEAPGRGGHRGGSDGR"
BASE COUNT         1830 a         4286 c         4552 g         1808 t
ORIGIN      
        1 gccgcccacc ggcggcccca catgtagtgc atcatggcgc gatcgccgcc gggagtccat
       61 ggcccggcgg gcggggccat catcatccgc atcatgccgt gcgagggctc gggtggcgcc
      121 cacggccggc cgtcgcggtc gaggaggtcc acgcggctca gaccgttctg ctcggtgacc
      181 cgcgcgagat ccacgatccc gatggggcgg gccagcagcg cgtcgacgag ccgcgcgttg
      241 tccaggaggc gctgggcgat cgtctcctcc atcagggcgt tgctccggat ggcgttgcgg
      301 ctgccggcct cgacggcatc ggcgacggcg agccctctct gctccatctg gacgcgcagc
      361 tccacctggc ttcgatgggt cgtccacgcg gcatgggctg acaccagcgc gaagagcagc
      421 gccagcgagg cgaggaagtg gcgcggcgag agggtcatgg cttcggcgga gccaggcgcc
      481 ggcccccgag cgcctcgggg gccgggttcg agaagacgtc ctcggccgtc agtccgtggt
      541 cgcggccagt gggccgaagg ggcgcacgtc gccccgcggg gtgacgtgca ggtaacgggt
      601 ctcgcccttc gggccgcgca gctccacctg gtacatcgtg cgggaccgcc cgatgaacgg
      661 cagcacccgc tcgatcgtga agcccttgaa gtacttggtc acatattcgt tcgcgagctc
      721 cctggccttg tcctccgtca cggcctctgt gccggcctcc gtgccggcgg cgccccacat
      781 ggggcacgct tccgggccac ccgccatcgg gctccggccg gccatcattg ggctcctccc
      841 gcccatcatc gggccccagc cgggttcgtg ggcgagcgcc actccggcca gcatgcccac
      901 catcacgact gcgaacatca cgctcaccac cttcttcatt cgactccccc cctttcaacg
      961 tcgctcgcac ggacagtggg gcattcaccg tgccagtccc gcagccgccg gggaggtcgt
     1021 cacgacgcgg acttacgaca cgaacccagc cacggcccat ggagcatcga cgattccgtc
     1081 gagatggctc gacgatctcg tctgctggcc gccgatgact ttcacgccgc ctcacgcaac
     1141 gcctcgccgt cagccagcag tgcgtagcgc gcgaccgcgg acgacggcgt ccacgacgtg
     1201 tacgccgagc cgaggcagcc cttggcgcag cggacaccgt ccgtgaacat cgagcaggcg
     1261 acgacgtcgg ccggccggga tgccccggcc cgcgtgacga acgccgcggt caccggcctc
     1321 cgggtcaccg ggcacgtgaa cgaggcccgg gccagcgccg gcgcgggcgg cgccagcagt
     1381 gacagtggca acagcacgag gaacgtgccg agcaggagca cgaccggcgc cgcgatcagg
     1441 acgagcacca gacccgcgac gaacagagaa acccattcca tgtcgtttca ccatccttcc
     1501 ggcggggcca tgctgcagcg attccgccgg caatgaagac tgcaagcgat gtaccgcggc
     1561 cggtgggcga aaacgtacgc tggatcagaa gcttgcgggc ccagtcccgg gcgcgggctc
     1621 tcccgggctg gggcctgtca tcccctcgtg gggcgccagg gccccacgag gggcccggtc
     1681 acttgaacac gtagcggacg gtggctccga cgatgtgcgc ggtgtagttc cgggagtcgt
     1741 tgcccaggcg gattgagttc gagccgggca cgaacgggtt cagcccgtcc gtgcgccagt
     1801 cgctcccctt gaacgtctcg aaggcgtagc cgaagctcac cgcccactgc ctggtgaccc
     1861 agtagcggaa cgcggtgtcg agcctgacca gcatgtcctc gaaggccggc tgccgccggg
     1921 ccacggcgtt ggccgggttg ggaaagccgg gcgggtgggg gagcggggcc gtctcgttgc
     1981 gggtgtcgac ctcgccgaac gcgtagctcc agccggcctg gaccgtccag tcgagcttgt
     2041 tcgggatgat cccgaccttg gcgccgatgt gcgcggtctg cacgcgatcg aggttcaccg
     2101 acagccagtt ccagtcctcg aagtcggaga cgatgctctg gcccgccacc gtggcgacgt
     2161 cgcggttccg ggacgcgaac ttctggtaga tgtactcgta cacgtagccg gcgaacagcg
     2221 ccacccgctc ggtcggcgac cagctgacgt cgaagcccgc cgaccacgtg gtggcgtcac
     2281 ggaggccgaa ctggccgccg aggtagtcgt cgttgcgcca catgccgttg gcggtcagcg
     2341 tcacggtgtc gagcggcgtg aactgcagcg tcaggtcgag ctgctggcga tcgcgctcgc
     2401 cctggtcgaa cttcctgagc aggagcgact ggctctgcgc ctgctcggtc ggcgacagct
     2461 cctcctccag gacggtgtgg gcgatgtgcg cgaaggtgtt gtactcgtcg atgcgccgcc
     2521 aggagggctt gtaggtgagc cgggtcagca gccactccag cggcgtccag tcgagtgcgg
     2581 ccttgaggta gtactcgtcg ctcttcggca cctcgcggtg ctcctcgtca cgtttccagt
     2641 gctcccagcc gccgccgagc gtcgtggcca ggccgctgat cggccggtac tttgcgtcga
     2701 ggtccaggtt gtgacggcta tggtcgaagg cggaggtcct ccgctcctcg ttggacagcg
     2761 cctgatcgtt gacggcgtga ccggggaaca ccacctcgtc ggtcatctcg ctgtggtcga
     2821 agaagcggta ccggagactg agcgtcgtgc gcgggatcgc gcggttcacc aggctcaggt
     2881 tgatcgccgt gatgccgacg attccgtcga ggctctcctg cggcagcgtc agcagcgccg
     2941 tcgggatgtt ggggttgatc gtgtgcggca ggaagtcgtc gtcctgcagg cgcaggctgt
     3001 aggagaagcc cgaggaaagc cgcgtgcgcc acggcaggtc cacgccgccg gagatctgga
     3061 tcgtatgggc catgttgtcg ggggccagcg acgaccggcc gaacgccggg ctgctgctcc
     3121 ccccggtggc gctgggggcg aacgccgcga acaccgagcg caaggggttg tcggcgatca
     3181 cccgatcgtt ggagttctcg aaccggctga agatgtaacc ggcctggagc tgccaggtgt
     3241 cgcgggcgat ggtgccgcgc agacggaagt cgtggatggt gtggtcgatc ggctcgagca
     3301 cctcgatgaa gtcgttgccc gggctgccca tcgccatgcc gaagggctgg gtaccgtcct
     3361 tgtggatgcg cgcatagtcg gcgcggaagt cgaggtcggg ccgcggcgtg accagcagcg
     3421 agatccgcgc ggtgtcccag cgcacgccga cctcgtccag ctcgggggcg gcgttgtaga
     3481 gggcgccctc ggccgccgag atcgaagtgg cggtcggccg ggcgggcagc cgccactcgc
     3541 cggggctcac ctcgacggcg cgcgtgcgtg cggtcgtcga cagaaggtgc ggcgtctggt
     3601 cccaggcgaa gccgaactcc cagaggccga gccggccggc ccggagcacg tactcctggt
     3661 cgtcctgccc ccacttgctg cccccgagag tgaccgagta gctctcgtca gggcggaaga
     3721 accgcagctg gaactcgccg aggaacggtc cctgatcgat atcgcggtac tcctcgaact
     3781 tcccccggcg gctcggcgag ggccgctcga tgaaggcgcg tacgccggcc tcgacgtcac
     3841 cttccaggtt gaagccgccg agcgagagct ggccgctggc ggatggcgcc cacgccagcg
     3901 ttccgaccac gagccctacg ccgccgatga gccaccctgc cgcgcgctgg attctcatgg
     3961 cgccccctca ccgggtgaag gcgaagcccg atgggtgatt cgatccgtgg atgttcacgt
     4021 gacagttcgc gcagctgcgc ccgagcacga atctcaggtt ggcgggcgcc tcgcggccgt
     4081 agggccgcgt ggggtgccgg gtttccacgt ggcactgctg gcagagccgc ggcttggcgg
     4141 tcttcagcat gcgctcgtgg ttcgagccgt gcgggtcgtg gcagttgacg cagctctcca
     4201 cgacgggggc gtgctcccag aggaaggggc cgcgcttctc cgtgtgacag gtgaagcacg
     4261 agtcgttgat cgacggctcc ctgagcagcg cttggctgat ggtgccgtga gggttgtgac
     4321 acgacgtgca cgtcatcttg ccttcgcgca gcggcatgtg ggaggaacgc atctgctggg
     4381 cgcgccgctg gaggtgacag ctgccgcagg tctcgatctc ggtggccttg gccagctgcg
     4441 ccttcgacga gtggtcggtc atcacgcggt ggcagcccgt gcacgacacg tcgcgcgcgt
     4501 cgtgggcgct gccgctccag aagacgcgtt ggcccttgct gtggcagtcg agacagacct
     4561 tgttgcggat ctcgacgggc gtcggatcgt ccttcgcgaa cgtgatgagc ttcgcgtcct
     4621 tgccgcggcc cggggactcg gcgtgggcct tgcccggtcc gtggcagctc tcgcacgcgt
     4681 tcttctcctg cgcgctgcgg gggtgtctga ggaagagctt gcccatcttc gtcgcggcga
     4741 actggtggta ggactcctcg tgacacgcct tgcacgtctc ggcgccgacg tagtcggcgg
     4801 ccgacggggg ggccggcggc tgggcgacgg gccgtggtgt gtccgcggcc ttgaagcagc
     4861 cggtgaggag gagcgcgacc atgaggggaa ggaagaggtg atgcagcgcc ccacgccgtc
     4921 catggcgatc acgcatcgcg agcctccggc cggccaagga gtccggcgtc gatgcagcgg
     4981 ctggctaggg atagcagaga gcgcggtcca ttcgcaacgc ctttttgtcg acaattttgt
     5041 cgcgcgaccg ccgaacgcga ttcacagacg gcaaccacct ctccggggcg tcgcccgcgg
     5101 gcccggcatt gatctagcgg gtcgccgggg gcacgaactt cgaggcggtc gccagctgct
     5161 cgagcgtcag gacagcctcg taccgctggt cctcgatcgt ccaggtcggg tagcgtttga
     5221 caccggtccg ggcgcaacgc tcgggccggg cgccaggggc ccgggaatcg cactcgatgt
     5281 agggcaacag gtcggcggca ggcccgaacc gggtcttctg ttccccgcag gcgggtcacc
     5341 agtacgcgcc gtagaagatg gcgcccgagg cgctcaggtg acgggcgagc gcctcacgat
     5401 actcccggtg ctcgctcggc gcgtcagcga cgaacacgcc cgcagcgaag accacggtgg
     5461 cgacggccgc cccgccgcca accagtgcca gacgccccgg ccctgtctgt cgtcgcctcg
     5521 ccggggggcg gcgcagcagg accgtcacca cgagcgcgac ggcgacgacg gcgtcggcga
     5581 cgcaccaggg gcagatcgcg cgcaccacga agagctgcag gtacgtgaga tacgacgaga
     5641 acgcgagggc ggcggccgcc agggcaaaga cggcgagcca gcgccgcacg gccaggccca
     5701 cgagcgccag ggccgtgacg accgcgtaga gggcggcgcc ccacgccgcc gtcggcaacc
     5761 ccaggaagat cgcgtagcgg ctcaactgca cgatgtcgca gccaccaccg gcctcgcaca
     5821 ggagcgcctg accgccggcc agcttggcga ccgcgaggtg ggcggagatg gcgagcccgg
     5881 cggcggcgac cgcagcgacc ggccagtccc gtggcgcggc ctcccgtttc gtgctcgctt
     5941 tcatcgcgta ctggacgcag aatacccgat cgtcactacg gcgcatcgaa caagaaggag
     6001 gaggagatca tgaaccgagt cgcaggatgg agcgtcgtcg ccgcggtcat ggccgtcagc
     6061 ctcggcgccg gtccggccat ggccgaaccg gcgatgctca agcaggcgaa ggacgcgggg
     6121 ctgccggcgc agaactgcca gtactgtcac accacgaaga tcccgaagaa ggagaccttc
     6181 aagcccgagg agctcaccga tcgcgggaag tggttgcggg cacagaagga gaagcggaag
     6241 gcgccgaagg ccgaggcggc gtggctcaag gagtacccgg gaggccccgc gcagaagtaa
     6301 cgcgtcaggg tcagcgcgcc gacacgcggg cacgcgcctg ctcggccttt gcgcgcgccg
     6361 tcgccagcag cgcgtcggcg gcgagggggt tgtgcgcgac gccggcgccg cgcacgaggg
     6421 cgagggcgct gcgcgcctgc cggaggagcg catcggcctc cctgaccgcg cggccggcgc
     6481 gcttggcctc ggccagtgcc gcctccgctg tccggaccgc ctgggcggcc cggcgcacgt
     6541 cgttccggta gccggtcgtc cattctcgga gcaaggcgac gtacggcgcg tcgtggcagt
     6601 tgaggcaggc cttgccgaca gcctcgcgcg agtgcgtgcc gctgaaggcg tggcaccccg
     6661 tacacggcac cgcgtccgcc atgaggttgg gggcgatctt cgcgtccggc gtgtgcgccg
     6721 tgccgcggta gaacgccgcc tgggccgagt ggcacgtctc gcagcgatcg ttctgggggc
     6781 cgtggtggca gccgcggcac gtcgccggcg tcgcggtgac ggccttgtgg gtctcggcgg
     6841 agtggcacga cgtgcacgtg gcgccgaaat cggtgacgtg gcgcgcgtgg gggaagcggc
     6901 gctgctggaa cgtcgccgtc gcgaggacct tgacgcccgc gacctggtgg cagagcacgg
     6961 cgcagtaccc gccccggacg agctggtcgt cggtcttcgg cgccggcgcg ccggacgcgg
     7021 cgatcgcctc gtcgagccag ccgttggcca gcttgaggag gtcggcggcg tagaagacgt
     7081 tgtgcacgcc cttggcgagg ccgacgaagc ggacgttgaa ttcggcgtcc tccacgagct
     7141 tccgtgcccg cgccagcttc gggctcttcg ggtcgccgcc cagcgcggcc cgggccgcgg
     7201 cgagcttcgg cgcgatcgcc tggcgcatgt gttcgagggt cgtgccccac tgtgcgagca
     7261 tccctcggta cttctcgccg tggcagccca ggcacgcccg ctcgctcggc cggaacgtct
     7321 gcccggtgat ggcggccgct ccggccgcgg acgcgggggt gccgtggcag gcgacgcact
     7381 ccacgcgcac ctggaacatg tggctcggga tcatcggcgt cccgcggccc cccacgccga
     7441 cgtacatctg cgcgacaccg cggtgctttc cgatgtggca gttgcggcaa tcgagctcgg
     7501 agtcgccgtg gccagttgcg ccaccgggcc ccgcggacgg tgggcgagcg ggcccggacg
     7561 gcggcggggc cggggagggc gccgtctcgc gtggcgagcg cgtctgcgcc ggcgcggccg
     7621 cgccgccgac gagcgcggcg gcgagcgcga gccacgtcac cgtacgcttc accggccgcc
     7681 tccgggcccc gtcgcgagga cggcgagcgc cggcggcgcc gcgtccgctg tcggcggccc
     7741 gatccgcgga ggcagccgat gcttgatctc gctgtggcaa cgcgtgcact cgatggagcg
     7801 ggcgctgacg tgggcggcat ggaccaggcg cgcgtcgtcg tacttcgcga tcacctcgcg
     7861 ctggttgtgg catcccatgc accgctcccg cggcgcgtcg ccgcgcccct cgacgacgct
     7921 gaggtgacag ctctgacagg cgacgccgcg ctgcgcgacc tcgtcatgat tgaagcggac
     7981 ggatccgacg acgatgtcgc ccctgggaac gccgtggcag ccagtgcagc cggcgacggg
     8041 gatgagttcg cgcccgttcc ggcgggcctt gaagtggcac gtgaagcagg cgctctgcgt
     8101 gacctcgaag tggcgatcca ccaccatctg cgcgtgacag ctcgtgcagc gcagctcgcg
     8161 gcccatggcg gcggtgaggt ggcgggcgtg atcgaagcgg atcccgcgcc gggacgtcag
     8221 ctcgcctttg cgctcgagat cggtccgggc gtggcacccg gagcgcaggc agctggcgtc
     8281 ctcgacctcg gcgaagggct tggagctgta ggtgccggtc gcccacttgg ctacctgggt
     8341 gacggcctgg aacttcacgc gaagggtgtc ccgaagcccg ggcggataat ggcactccac
     8401 gcacgctacg gccgcgtgtg acgatgcctt ccacgcgtcc acatacggcc gcatgatgtg
     8461 gcacgagttg cagaaccgcg ggctcgcgct gacgtgccag agcccggcgc cggcggcgac
     8521 gccggcggcc agcacgatgg ccgcggcgag gaggatcact ggaccgcgcc agcgtcgccg
     8581 cggcgtccgg cctcctcccg gcgcgtcgtc actcatcgac cgtcaaggct ctgcggcccc
     8641 tcacttgtga caacggtcgc actcgtcgat ggaaaacacg acggcgccgg ccatcatggt
     8701 cttgccgtcg tggcaagctc cgcagaaccg tccctgctgc ttggcctcca gcgtgatcgg
     8761 tccggacgcg ccgcgcttca tcttgaggtc ccgcatgtgg cagcgcgtgc acttgcccac
     8821 cttcccgaga tgagccgcgt ggctgaaggt gaccgggccg gggctacctt ctccgggctt
     8881 gatcgcgaag tcgggcggag ccttcccttg ggcgaacccc ggcccccggt cgaggccggg
     8941 ccccgccagg aggaagccgg tggcgaggac cgcgacgacg agcgcgccgg gacgggtggg
     9001 agtcaggggg cgccccggcc gctgcgcaag aatcccacga ggttgatcag ctcgtcgccg
     9061 gtgaagcgcg gatagaggaa gccgcgctgc agcgcgaccg ccgccatccg cggcgtgtgt
     9121 gtccacatcg cggcggccca tgccgccggc gcgccgtagg cgggccgctg gtcggcgaga
     9181 tcgggtccga ggcgggcgcc ttcccgccgg aaggcgtggc acttcagaca tcccttggcg
     9241 atcagcgtga gctggccgcg caccaggtcc ggggccggat cgcgggccgg gtgcgccgac
     9301 aggtaggcca tgaggtcggc catctccggg acggtgatct gcggccacgc gaggccctct
     9361 tgcgtgagca gcatgaacat ggccggtgca tggttccaca tgcgccccgc gagctcccac
     9421 gacccctgcg ggcgacgcag cgtctggagc gccggcatgg ccccggaggc ggcggtgcgg
     9481 tggcagcgcg cgcacgcctt cgcgacgaac agctgctcgc cgcggacggc atctccagtc
     9541 ggttgcgcgc tcgcggtcgg cggggcgagc acgggagcga ccgggacgag cgccggcgcg
     9601 acgagcgcga ctgcccacgc gaccgcggcg aggctgcgcg cgcgacgccg gcccgggccc
     9661 gcgccggtca ccgcctggcg tctcatttcg tcgggctggc cgtatagccg aggtaggtga
     9721 gcgcgagcat gtagacgatg atcgccaggc cgatggcggg gaggactcgg ttgggtgcgc
     9781 cccggccggc cccgcggtcg aagaacggaa tcagcagcag gaagaggccg cccagcccga
     9841 agccgagcac gccgatcagc tcgccctcga tgccgaggat gtggcccggc aggtacttga
     9901 gcgtctggaa catgaacatg aaataccact cgggcttgat cccggccggc gcggccgcga
     9961 acggatcggc cttgcggccg agctcggcgg gccagaaggc cgcgagcgtg gccagcgcgg
    10021 ccagcgccgc caaccagccg acgaggtcgc gcaggaagaa gctggggaag aacggcatca
    10081 tccgccgtgg cccgccggca cgctcgaccg aggggggcac gctcatgccg tgcttctgca
    10141 cgaggaacag gtgcagcccc agcagcgccg ccgcgatcgc cggcagcacc gccacgtgga
    10201 tgccgtagaa gcgcgtgagc gtggccccgg tcacctcgtc gccgccacgc agcagccgcc
    10261 gcaggaagcc gccgacgacg ggcagcacgc cggggatctc cgtgccgacc ttcgtcgcga
    10321 agtacgccag ctcgttccac ggcagcaggt agccggtgaa gccgaatccg agcgccagcg
    10381 ccaggagcac gacgcccgag atccaggtga cctcccgcgg cggccggtag gcccgcagga
    10441 gcatgacgct gaagaggtgc acgaagacga cgaagaccat caggttggcc gcccacgagt
    10501 ggatcgagcg cacgagccag ccgaactgga cctcggccat caggaactgg atcgactcgt
    10561 acgcctcctc ggcgctcggc cggtagtaga acagcagcag gattcccgtg ccgacctgca
    10621 ccacgaagag gaacagtgcc atgccgccga agtagtacca gagcgagtgc cggtgatcgg
    10681 gcacctcctt cttcttggcg aggcgctgga tctcggacag accgacgcgc tcgtccagcc
    10741 agcgccagac ggggccgcgc gccctgggct cggcaggtgc caccgggggg ctcacgtgct
    10801 cttgctcacg acgatctggt cgccgcgcac gttcacgacg aacgtgtcga gcgggcgcgg
    10861 gggcggtccc tcgatgttct tgccgttgag gtcgaagtgg ccgttgtggc aggcgcacca
    10921 gatgtgaccc aggtcggcac ggaactgcac cgtgcagttg aggtgggtgc agacggccga
    10981 gaacgcgcgc agctcgcccg ccggcgtccg cacgaggatg ccggggcggc tgccgaactt
    11041 gaagatctgt ccagtgttgg gcttcacgtc cgccgccttg aggggcagcg tgaccgtgcc
    11101 ggccgtcgat tcaccggcct cgggcggaac gaggtagcgg cccaccggat agagcaccgc
    11161 cacgaggaag gctccgaggc tcgtggccag gaaccagttg acagccctcc ggctcgcgcc
    11221 gaggtccagg gtctcatcgg ccatcgctgc ctcctcggtg tccgccgcgc ccgggggctt
    11281 cgatctggcg gatcttcagc accacggccg cgatcagtgc gaggatcacg accagcgaca
    11341 cgcccagccc ggcgcgtcgg aaccgcagct cgtccatcgc gcggagccca cgctggagag
    11401 ccttctcggc gatggtcaga cccggcgcga cctccttgtc gaccgcggac gcggtgaacg
    11461 cgtgcacggc tgcccgcgcc ttcaccagcg cgtcggccgc cccgttgagg tcgaacagcg
    11521 cctggctgac ctccatgccc gactgctcgg cgtgcgtgag cacgcttcgc gccccatcga
    11581 cggcggagtg cagcgcgtcg attcgcccgc gcatggcgga ggccacgcgc ccaccggcgt
    11641 cgtcggcgcc atggcacgat gcgcacgccg cgtccttgcc cacgccgagc aggtcgtcac
    11701 tggcccgggc gatggcgtgg ttgccgtggc aggtggcgca gcccgggctg cccatgtcca
    11761 cgagcacctt cgcgtgcgcg ctcgccctga agaggtcggc catcacggcg tggcactggc
    11821 cgcagacgtt gccgacccag gatacgccgg gcggcgcggc gccgtggttg ccgtggcagt
    11881 cgttgcaggt cggcgccgag aggtcgccct tcgccgacat cgcctgccag tgcacgctgg
    11941 tccggtactt ctcgagctga tcggtgggga tgcgataacc tttcatgtac gcggcgtcgg
    12001 cgtggcagcg accgcacgtc acggccacct tcatcgggtg cacgctcgac ttggggtcgg
    12061 acgccggtcg gatggagtgg gccgggtggc agctcgcgca cacggcgacc ttctggtcgc
    12121 cctgccggag gcggcgtccg tggacggaag tcaggtactc cgccacctga tccacgcgca
    12181 gggcgggatt gtactgccgc atgaactggg cgtcggagtg gcagcgcccg cacacctgcg
    12241 ccatctgcgc ccgagcgggc ttgccgatgt agcccttggc ggggtccatc gcctccatcc
    12301 cggcggcctt ggcgtcaccg ccatggcagg ccacacagcc gagcccccgg ctcctgtgca
    12361 cgtcctcggc gtaggccttc gccggattca cgaggcggtc gtcaccgatc tccaggtggc
    12421 aggccgtgca ctggtccgcg gccggcgcct gcgctccggc gcgcgccggg acgagc
//
LOCUS       DATIAZ010000202         5798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1500934, whole genome shotgun sequence.
ACCESSION   DATIAZ010000202 DATIAZ010000000
VERSION     DATIAZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500934"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VLF19_07205"
     CDS             <1..710
                     /locus_tag="VLF19_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE93077.1"
                     /translation="IGQSEGSWIRSSLGLRYLAVRPTLAEFVLEMPRGAQVIYPKDLA
                     MILFWADVFPGAHVLEAGTGSGALTLALLRAVGPDGRVITYEQREEFARRALANIHMR
                     AGEVTNLTVRLGPVEDGIGDEQPVDRVVLDLPEPWTLTRAVAGVLRPGGIFLAYVPTI
                     IQSHQLCEVLHRDPHWALVETFETLVRPWNIEGQSVRPFHRMVAHTGFITVARRVVEG
                     EGGPATRLRAAEGDRAE"
     gene            747..2630
                     /locus_tag="VLF19_07210"
     CDS             747..2630
                     /locus_tag="VLF19_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSE93078.1"
                     /translation="MRRYRRGLQVGLLIVIAAFIASLFVFGATGGRDGVVAPDSVATV
                     NGESISQERYHRRYQEYLNLYQQTLRERFSPDLAERMGMPRQVVDDLVQETLVAQRAR
                     AEGLEVDDEELNAQIHAMPAFQDGGRFTLKRYEDVLRRLGYNKTSFEDDMRRRLTRLK
                     VESMVRGAVRVSDTELEQAYTHMREEVRAAWALVETAPLATAATATDAELETYLQQHG
                     AEFRLPERRRVQYVAVDPKDFATPPADAEVEKYYAEHAAEFEQPPQVRAAHILVRVPE
                     TGGSEAEDQARAKVADAIRRVKAGEDFGKMAREMSQDPTTGPRGGDLGFVSRGEVVKP
                     FEEALFALKKGEITAEPVRTPFGFHAIKVDDVRPGGKKTVKEVAPQIRERLKTQAADA
                     AAKARADEIRAKLLGAADFMAEARKLGLAPVESTIPRREPEGFAPPDTMEETAFGLTR
                     GGVSTPLKTPAGFVVLKAAETLPAAVPPLAEVKDRVAAAVKLQKAEAEALARARRVLT
                     EAKAGDLAAAARAAGATAGETPRFSRAKPAERLPGDAMLAALKTPVGALTEPVKTPQG
                     YYVLKTLERVPPDMAGLAAERDKVAAEVLGRKQSQAWEAWLAGARTKAKIDVSSRLPS
                     RRG"
     gene            2851..3201
                     /locus_tag="VLF19_07215"
     CDS             2851..3201
                     /locus_tag="VLF19_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSE93079.1"
                     /translation="MTIVGQTAKVALVCFGAVTALGTVGVNVTALVAGLGLTGFALGF
                     AFRDVLSNLLAGLLILLFRPFTRHDRIAVTGFEGTVAAVDLRYTTLEREDGRVLIPNS
                     ILFTNPIALRARRE"
     gene            3274..3756
                     /gene="greA"
                     /locus_tag="VLF19_07220"
     CDS             3274..3756
                     /gene="greA"
                     /locus_tag="VLF19_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSE93080.1"
                     /translation="MSVQRTPMTRSGYERLAAELERLKRVERPAITRAIAEARAHGDL
                     SENAEYHAAREKQSFIEGRIGELETKVGAAEVIDPPTQGPRITFGSTVLLEDETGKQH
                     RHWIVGSDEAEPARGKISILSPLARTLIGKSVGDTVTAQLPGGTKTFDIVKANFPWTD
                     "
     gene            3769..4665
                     /locus_tag="VLF19_07225"
     CDS             3769..4665
                     /locus_tag="VLF19_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE93081.1"
                     /translation="MPSARVDGVNLWYEEVGQGTPLVFVHEFAGDARSWHLQVRFFAR
                     RYRTIAFNARGYPPSDVPDDRAAYSQDRAADDIKGLLDALRIDKAHVCGLSMGGYATL
                     HFGLRYPNRARSLVVAGAGYGSVPGDRAKFHQDAEATAQRFERDGMKVTADLYAKGPT
                     RVQFMDKDPSGWREFYEALTAGSARGHALTLRGVQLTRPSVFDLGDRLERLDVPTLIM
                     TGDEDEPCLEPALFMKRKIPTAGLVVLPKSGHTINLEEPEMFNRAVLDFLTAVDAGRW
                     TRRNPASQTGSAILPADAADRR"
     gene            4662..>5798
                     /locus_tag="VLF19_07230"
     CDS             4662..>5798
                     /locus_tag="VLF19_07230"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE93082.1"
                     /translation="MSPLPLDHIVVVDLTRARSGPTAVRQLADMGARVVKVEARELVA
                     GDSTDLGFDYLNLHRNKRSMTLDLKHPRGVEVLKRLVGRADVLVENFRPDVKRRLGID
                     YETLSAGNPRLVYASISGFGQTGPYAERPGYDQIAQGMGGLMSVTGLPGQGPVRVGIP
                     VADLTSGLFLAQGILVALIEREKSGRGQWVHTSLLQAMVTMLDFQAARWLIDGEIPPQ
                     AGNDHPTGIPTGVFTTADGHINIAASGQVMYRRLCEAIGAKELIDDPRFRTLADRSKN
                     RKAMNEALDGVLVSRTSAEWIELLNQAGVPTGPIYNVQQVFEDPHVRTLGLAQPVTHP
                     ERGEIRVQGLPTTLSRTPGSIRRPAPTHGEHTDEVLRELDYSADD"
BASE COUNT          873 a         2061 c         2093 g          771 t
ORIGIN      
        1 tcatcggcca gagcgagggc agctggatcc gcagctcact cgggctccgc tacctggcgg
       61 tccgacccac gctggcggag ttcgtgctgg agatgccgcg cggcgcccag gtcatctatc
      121 cgaaggacct ggccatgatc ctcttctggg ccgacgtctt cccgggcgca cacgtgctgg
      181 aggcgggcac gggctccggc gcgctgacgc tcgcgctgct gcgcgcggtc ggccccgacg
      241 gccgggtcat cacctacgag cagcgcgagg agttcgcgcg ccgcgcgctc gcgaacatcc
      301 acatgcgcgc cggcgaggtc acgaacctga ccgtgcggct cggccccgtc gaggacggca
      361 tcggcgacga gcagccggtg gaccgcgtgg tgctcgacct gcccgagccg tggacgctca
      421 cgcgggcggt ggccggggtg ctgcggccgg gcggcatctt tctcgcgtac gtgcccacca
      481 tcatccagtc gcaccaactc tgcgaggtcc tgcaccgcga tccgcactgg gcgctggtgg
      541 agacgttcga gacgctggtc cgcccctgga acatcgaggg gcaatcggtg cgtcccttcc
      601 accggatggt ggcgcacacc gggttcatca cggtcgcgcg ccgcgtggtg gagggcgagg
      661 gcgggccggc cacccggctg cgcgcggccg agggtgaccg cgcagagtag gtccgtggcc
      721 gccgaggagt ccagatgatc ggattcatgc gtcgttaccg ccgcggcctc caggtggggc
      781 tgctcatcgt catcgcggcc ttcatcgcct cgctgttcgt gttcggcgcc accggcggcc
      841 gggacggcgt ggtggcgccc gacagcgtcg ccaccgtgaa cggcgagagc atctcccagg
      901 agcgctatca ccggcgctac caggagtatc tgaacctcta tcagcagacg ctccgcgagc
      961 gcttctcgcc cgacctggcc gagcgcatgg ggatgccgcg ccaggtcgtg gacgacctcg
     1021 tgcaggagac gctggtcgcc cagcgggccc gcgccgaggg gctggaggtc gacgacgagg
     1081 agctgaacgc gcagatccac gcgatgccgg cgttccagga cggcggccgc ttcacgctga
     1141 agcgctacga ggacgtgctg cgccggctcg gctacaacaa gacgtccttc gaggacgaca
     1201 tgcggcgccg cctcacgcgc ctgaaggtcg aatcgatggt gcgcggcgcc gtccgggtgt
     1261 ccgacaccga gctggagcag gcgtacacgc acatgcgcga ggaggtgcgc gcggcgtggg
     1321 cgctcgtcga gaccgcgccg ctggccaccg cggccaccgc gaccgacgcg gagctcgaga
     1381 cctacctgca gcagcacggc gccgagttcc ggctccccga gcggcggcgc gtgcagtacg
     1441 tcgccgtcga tcccaaggac ttcgccacgc cgccggcgga cgccgaggtg gagaagtact
     1501 acgccgagca cgcggccgag ttcgagcagc cgccgcaggt ccgggccgcg cacattctgg
     1561 tgcgagtgcc cgagaccggc ggcagcgagg ccgaggacca ggcccgggcc aaggtcgccg
     1621 acgccatccg gcgggtgaag gcgggggagg acttcggcaa gatggcgcgc gagatgtcgc
     1681 aggatccgac gaccgggccg cgcggtggcg acctcggctt cgtgagccgc ggcgaggtcg
     1741 tgaagccctt cgaggaggcg ctgttcgcgc tcaagaaggg cgagatcacg gcggagcccg
     1801 tgcgcacgcc gttcggcttc cacgccatca aggtcgacga cgtgcggccc ggtggcaaga
     1861 agactgtcaa ggaggtcgcc ccgcagatcc gcgagcggct gaagacgcag gcggccgacg
     1921 cggcggcgaa ggcgcgcgcc gacgagatcc gcgccaagct gctgggcgcg gccgatttca
     1981 tggccgaggc gcgcaagctc ggtctggcgc ccgtcgagtc cacgatcccc cgccgggagc
     2041 ccgagggctt cgcgccgccc gacacgatgg aggagacggc gttcgggctg acccggggcg
     2101 gcgtatcgac gcctctgaag acgccggcgg gcttcgtggt gctcaaggcc gccgagacgc
     2161 ttcccgccgc ggtgccgccg ctggcggagg tgaaggacag ggttgcggcc gcggtgaagc
     2221 tccagaaggc ggaggcggag gcactcgcaa gggcccggcg ggtgctcacc gaggccaaag
     2281 ccggtgacct cgccgccgca gcgagggccg cgggggcgac ggccggggag accccgcgct
     2341 tctcgcgcgc caagccggcc gagcggctgc cgggcgacgc gatgctcgct gcgctgaaga
     2401 cgccggtcgg cgccctgacg gagccggtca agacgccgca ggggtactac gtgctcaaga
     2461 cgctcgagcg ggtgccgccg gacatggcgg ggctcgccgc cgagcgcgac aaggtcgcgg
     2521 ccgaggtgct gggacgcaag cagagccagg cctgggaggc ctggctcgcg ggagcgcgca
     2581 ccaaggccaa gatcgacgtg tcgtccaggc tgccgagccg ccgggggtag tcgttcgcca
     2641 ctgacatggg ggaccgaccc ccagcagtca gcagcgggga ggccctcaat ggaagatctg
     2701 gccagagaac tggcttcgga cgcgtcgcgt ctgaccctcc gtgtcgtcgc cggcgctctc
     2761 atcttcgcgg cgttctggat cggcgcccgt atcgtgcgcg gcgtcactcg ccgcgtcgcg
     2821 gaacgcagcg aggtctcccg gcaggacgcc gtgacaatcg taggccagac ggcgaaggtg
     2881 gcgctcgtgt gcttcggcgc ggtgaccgcg ctcggcaccg tcggcgtcaa cgtgaccgcg
     2941 ctggtcgccg gcctcgggct caccggcttc gcgctgggct tcgcgttccg cgatgtgctc
     3001 tccaacctgc tcgccgggct gctcatcctg ctgttccggc cgttcacgcg gcacgaccgg
     3061 atcgccgtga ccgggttcga gggcacggtg gcggccgtgg acctgcgcta caccacgctc
     3121 gagcgcgagg acggccgcgt cctcatcccg aactcgatcc tgttcacgaa tccgatcgcg
     3181 ctgcgggcgc gccgagaatg accggtgatc cgccccgacg ccggggcgga ggggacgcgg
     3241 caccccgtcc ggggccgtgg taatttacct ggtatgtcgg tgcagcgcac acccatgacg
     3301 cgctcgggct acgagcggct cgcggccgag ctggagcggc tcaagcgcgt cgagcggccg
     3361 gccatcacca gagccatcgc cgaggcgcgc gcccacggcg accttagcga gaacgccgag
     3421 taccacgcgg cccgcgagaa gcagagcttc atcgagggcc gcatcggcga gctcgagacg
     3481 aaggtgggcg ccgccgaggt catcgatccg ccgacgcagg gcccccggat cacgttcggc
     3541 tccacggtgc tgctcgagga cgagacgggc aagcagcacc gccactggat cgtcggctcg
     3601 gacgaggccg agcccgcgcg cggcaagatc tcgatcctgt cgccgctggc gcgcacgctc
     3661 atcggcaagt cggtgggtga caccgtcacc gcgcagctgc ccggcggcac gaagaccttc
     3721 gacatcgtca aggccaactt cccctggacc gactgagagc ctgacagcgt gccctccgcc
     3781 cgggtcgacg gcgtcaacct ctggtacgag gaggtcgggc aggggacgcc cctcgtcttc
     3841 gtccacgagt tcgcgggcga cgcgcggagc tggcacctgc aagtgcggtt cttcgcgcgc
     3901 cggtaccgca ccatcgcgtt caacgcgcgt ggctacccgc cgtccgacgt gcccgatgac
     3961 cgcgccgcct actcgcagga ccgcgccgcg gacgacatca agggactgct cgacgcgctc
     4021 cgcatcgaca aggctcacgt ctgcggtctg tccatgggcg gctacgcgac cctgcacttc
     4081 gggctgcgtt atcccaatcg cgcccgctcg ctcgtggtcg ccggggccgg ctacggcagc
     4141 gtgccgggcg accgcgcgaa gttccaccag gacgcggagg cgacggcgca gcgcttcgag
     4201 cgcgacggca tgaaggtcac cgccgacctc tacgcgaagg gccccacgcg cgtgcagttc
     4261 atggacaagg acccgagcgg ctggcgggag ttctacgagg cgctgacggc gggctcggcc
     4321 cggggccacg ccctcacgtt gcgcggcgtg cagctgacgc ggccgtcggt cttcgacctc
     4381 ggcgaccgtc tcgagcggct ggacgttccc acgctgatca tgaccggcga cgaggacgag
     4441 ccgtgcctcg agccggcgct cttcatgaag cgcaagatcc cgacggcggg gctcgtggtc
     4501 ctgccgaagt cggggcacac catcaacctc gaggagcccg agatgttcaa ccgcgcggtg
     4561 ctcgacttcc tcacggccgt ggacgcggga cgctggacgc gacgcaatcc cgcctcgcag
     4621 acgggctcgg ccatcctgcc ggccgacgcg gcggaccgcc gatgagcccc ctgccgctgg
     4681 accacatcgt cgtcgtcgac ctcacgcgcg cccgctcggg gccgacggcc gtccgccagc
     4741 tcgccgacat gggggcgcgc gtggtgaagg tggaggcacg ggagctcgtc gcgggcgact
     4801 cgacggacct cggattcgac tacctgaacc tgcaccgcaa caagcggtcg atgaccctcg
     4861 acctcaagca cccgcggggc gtcgaggtcc tcaagcgcct cgtcggccgc gccgacgtgc
     4921 tcgtcgagaa cttccggccc gacgtcaagc gtcgcctcgg catcgactac gagacgctca
     4981 gcgccggcaa cccgcgcctc gtctacgcct cgatctccgg cttcggccag acgggcccct
     5041 acgcggagcg cccgggttac gaccagatcg cgcagggcat gggcgggctc atgtcggtca
     5101 ctgggctgcc ggggcagggg cccgtgcgcg tggggatccc cgtcgccgac ctcacgtccg
     5161 gcctgttcct ggcccagggc atcctcgtcg cgctcatcga gcgcgagaag tcgggccgcg
     5221 gccagtgggt gcacacgtcg ctgctgcagg cgatggtcac catgctcgac ttccaggccg
     5281 cgcgctggct gatcgacggc gagatcccgc cgcaagccgg caacgaccat ccaaccggca
     5341 tcccgaccgg cgtcttcacc accgccgacg gccacatcaa catcgccgcc tccggtcagg
     5401 tgatgtaccg ccggctctgc gaggccatcg gcgcgaagga gctgatcgac gacccccggt
     5461 tcaggacgct ggccgaccgc tcgaagaacc gcaaggcgat gaacgaggcg ctggacggtg
     5521 tgctcgtgtc gcggaccagc gccgagtgga tcgagctgct caaccaggcc ggcgtgccca
     5581 ccgggcccat ctacaacgtg caacaggtct tcgaggatcc gcacgtgagg acgctcgggc
     5641 tggcccagcc ggtgacgcat cccgagcgcg gcgagatccg cgtgcagggg ctgccgacca
     5701 cgctctcgcg cacgccgggg tcgatccgcc ggccggcgcc cactcacggc gagcacaccg
     5761 acgaggtcct ccgggagctg gactattcgg ccgacgac
//
LOCUS       DATIAZ010000203         6218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_582203, whole genome shotgun sequence.
ACCESSION   DATIAZ010000203 DATIAZ010000000
VERSION     DATIAZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582203"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..665)
                     /locus_tag="VLF19_07235"
     CDS             complement(18..665)
                     /locus_tag="VLF19_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93083.1"
                     /translation="MTRVALSLVFALALVSATEALDVATADYQRAAAQGRVGVVVGRA
                     AEEARRRNAADTPLSGFSVTLVPRSTEFLARLRAIAHRGRTDPAAYALTAPAIAAARK
                     DYERALEEAGAGQLVKNRTVEADGRFEVRDVPEGEWLLVALRPVFVAKPSPPERKARE
                     KQAFTPQPRISGYYAVTVWLRELSVTVGRETEVQLTDRTAWMTAIEEKPVPGAGP"
     gene            complement(722..1564)
                     /locus_tag="VLF19_07240"
     CDS             complement(722..1564)
                     /locus_tag="VLF19_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSE93084.1"
                     /translation="MVGRLPRASAAWLGRRIGDALWLGLPGRRRLALNNLLRAFPASS
                     GPERRRMARRSCQHLGLLFVEICWLLARPLDQVLAGVRLEGREHLDDVMRKHGRALVL
                     TAHLGNWELLPAASRLTPYALTIVVRPLDAPWLNRLAEALRRKAGIELIDKRAALRPV
                     LGALARGRLVGILLDQNASRQEGVFVPFFGRPASTSRAVAVLALRTGAPVVPIFTRRE
                     PDGTHRVVVQPPIEVEAAGAPERAVTELTARCTAVIEAAIRETPEQWLWMHERWRTRP
                     APPP"
     gene            complement(1579..2211)
                     /locus_tag="VLF19_07245"
     CDS             complement(1579..2211)
                     /locus_tag="VLF19_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSE93085.1"
                     /translation="MEFLLDLATGRYALDDLVRWGGYVILVAIVFTETGLLIGCFLPG
                     DSLLITAGLVAATGALDIWSLNVLLTAAAIVGDSVGYAIGARLGPRLFTREKSLLFNP
                     RHVERTRLFYERHGAKTIVIARFVPIVRTFAPVLAGVGQMQYRRFLAYNVAGGAGWVL
                     SLTWAGFLLGHAIPDIDRYIHVVVGVVIVLSVVPIGIEILRERRRRSTSS"
     gene            complement(2216..4654)
                     /locus_tag="VLF19_07250"
     CDS             complement(2216..4654)
                     /locus_tag="VLF19_07250"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HSE93086.1"
                     /translation="MAIGPGPVDAAPSPQPHAGGGEASLVIPDLGQVPVGGMNGRTLL
                     WAGLGVCVLGFVFGLTMYSQVRQLPVHASMREISELIYETCKTYLITQGKFILLLEVF
                     IGVIIFTYFYALLHLDLARVIVILLFSLVGIAGSYGVAWFGIRINTFANSRTAFASLK
                     GEPFPVYAIPLKAGMSIGMLLISVELFLMLCILLFIPGDYAGPCFIGFAIGESLGAAA
                     LRIAGGIFTKIADIGSDLMKIVFNIKEDDARNPGVIADCTGDNAGDSVGPSADGFETY
                     GVTGVALISFILLAVREPAVQVQLLVWIFMMRVLMIVASGASYLLNDVIARARYAGAV
                     RMNFEAPLTTLVWLTSAVSVVLTYVASYTLISDLGDGSLWWKLSTVITCGTLAGAIIP
                     ELVKIFTSTESEHVREVVTCAREGGASLAILSGLVAGNFSAYWLGMVIAALMGTAWWV
                     STAGLGALMVAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVFELSVIETIPN
                     VTAEIRRDHGFEVNWERAKHLLEENDGAGNTFKATAKPVLIGTAVVGSTTMIFSIIVL
                     LTAGLTQNLDKLSLLHPPFLLGLVTGGAVIYWFTGASIQAVSTGAYRAVEFIKANIRL
                     EGSTKASVDDSKRVVEICTQYAQKGMFNIFVTIFCSTLAFAFFEPYFFIGYLISIALF
                     GLYQAVFMANAGGAWDNAKKIVEVELKEKGSALHAATVVGDTVGDPFKDTSSVAMNPV
                     IKFTTLFGLLAVELAVTLTAERGAGFSHALALGFFVVALVFVWRSFYGMRIRGGGPAP
                     AGVGGRVSEVARGK"
     gene            4890..5084
                     /gene="yacG"
                     /locus_tag="VLF19_07255"
     CDS             4890..5084
                     /gene="yacG"
                     /locus_tag="VLF19_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HSE93087.1"
                     /translation="MSDASPRDRGASCPACGRRVPWPGNPHRPFCSLTCRLVDLGVWL
                     DEGYRIAAEPFAAEGDRDVP"
     gene            5074..5898
                     /locus_tag="VLF19_07260"
     CDS             5074..5898
                     /locus_tag="VLF19_07260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSE93088.1"
                     /translation="MFRREVRTIICPSCGRLTREDAAECLVCGRRRPGLWGLTSLFRS
                     AFRSGSVTSVVTVVCVAVYLASLVLDPRGALRSRGLLGFLPIGDAAAALFAAGAIPWL
                     EGRWWTLLTAIYLHGNLLHILFNVLWIRQLGPAVEQLYGPARFITIFTVSGVTGFVAS
                     SLLGHAYTLGASGSIFGLLGAIVAYGQRRGGVFGKAVLREYGMFALILFVMAFVMPGV
                     DHFAHAGGFVGGLAAGYVLSIADRRGETTLDHLLAGAAVVLTVIAFALALWSGFTS"
     gene            complement(6084..6200)
                     /gene="rrf"
                     /locus_tag="VLF19_07265"
     rRNA            complement(6084..6200)
                     /gene="rrf"
                     /locus_tag="VLF19_07265"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1001 a         2137 c         2180 g          900 t
ORIGIN      
        1 ctcccagctc agctgagcta ggggccggcg cccggcaccg gcttctcctc gatcgcggtc
       61 atccaggcgg tccggtccgt cagttgcacc tcggtctccc ggccgacggt gacggagagt
      121 tcccgcagcc agacggtcac ggcgtagtac ccgcttatcc gcggctgcgg cgtgaacgcc
      181 tgtttttctc gtgccttgcg ctccggcggc gatggcttgg ccacgaagac cgggcggagc
      241 gccacgagca gccactcccc ctcgggcaca tcgcgcacct cgaagcgccc atcggcctcg
      301 accgtgcggt tcttcaccag ctgcccggcg ccggcctcct ccagcgcgcg ttcgtagtcc
      361 ttccgtgccg cggcgatggc cggcgccgtg agcgcgtacg ccgccggatc ggtccggccc
      421 cggtgcgcga tcgcgcgcaa gcgggcgagg aattcggtgg agcgcggcac cagggtgacc
      481 gagaagcccg acaggggcgt gtcggcggcg ttgcgacggc gggcttcctc cgcggcgcgg
      541 ccgaccacga cgccgacccg gccctgggcg gccgccctct ggtagtccgc cgtcgcgacg
      601 tccagggcct ccgtggctga cacgagcgcc agcgcgaaga cgaggctgag cgccacgcgc
      661 gtcatcgagc tccgcccgcc ggcgcgacgg gccgcgccga gcccaggctc actggaggag
      721 ctcatggcgg aggcgccggg cgggtgcgcc accgctcgtg catccagagc cactgttccg
      781 gggtctcgcg gatcgccgcc tcgatcaccg ccgtgcaccg cgcggtcaac tcggtcaccg
      841 cgcgctccgg ggcgccggcg gcctccacct cgatcggggg ctggacgacg acgcggtggg
      901 tgccgtcggg ctcccggcgc gtgaagatcg gcacgacggg cgccccggtg cgcagcgcca
      961 gcaccgccac ggcgcgcgag gtgctggccg gccggccgaa gaacggcacg aagacgcctt
     1021 cctggcgcga agcgttctga tcgagcagga tgccgaccag ccgaccgcgc gccagggcgc
     1081 ccagcacggg ccgcagcgcg gcgcgtttgt cgatgagctc gatgccggcc ttgcggcgca
     1141 aggcctcggc gagacggttg agccagggtg cgtcgagggg ccggaccacg atcgtcagcg
     1201 cgtagggcgt gaggcggctg gcggccggca gcaactccca gttcccgagg tgcgcggtca
     1261 ggaccagcgc ccgcccgtgc ttgcgcatga cgtcgtcgag atgctcgcga ccctcgagcc
     1321 ggacacccgc cagcacctgg tcgagcggcc gcgcgagcag ccagcagatc tcgacgaaga
     1381 gcaggccgag gtgctggcag gagcgtcgcg ccatgcgccg ccgctcggga ccggacgatg
     1441 ccgggaaggc ccgcaggagg ttgttcaggg ccagccgccg gcgcccgggc agcccgagcc
     1501 acagggcgtc gccgatgcgc cgccccagcc acgccgcgga cgcgcgcggc agccggccca
     1561 ccacggcggc gagcagcttc aggaggacgt ggaccgcctc cttcgctcgc gaaggatctc
     1621 gatgccgatc ggaacgacgg agagcacgat gacgaccccg accacgacgt ggatgtaccg
     1681 gtcgatgtcg gggatcgcgt ggccgagcag gaagccggcc caggtcaggc tcaggaccca
     1741 cccggcgccg ccggcgacgt tgtacgcgag gaaacggcgg tactgcatct ggccgacccc
     1801 cgccagcacc ggggcaaacg tgcggacgat cggcacgaag cgcgcgatca cgatcgtctt
     1861 cgccccgtga cgctcgtaga agagccgcgt gcgctccacg tgcctggggt tgaacagcag
     1921 cgacttctcg cgcgtgaaga gccggggccc caggcgagcg ccgatcgcgt agccgacgct
     1981 gtctcccacg atcgcggccg cggtcagcaa cacgttgagc gaccagatgt cgagggcgcc
     2041 cgttgccgcc accagcccgg ccgtgatcag cagcgagtcg cccggcagga agcagccgat
     2101 gaggaggccg gtctcggtga agacgatcgc gacgagaatg acgtagccgc cccagcggac
     2161 gagatcgtcg agggcatatc ggcccgtcgc caggtcgagg aggaactcca tcgggctatt
     2221 tcccgcgggc cacctcgctc acgcgtcccc cgacgcccgc cggcgccggc ccgccgccgc
     2281 ggatccgcat gccgtagaag gaacgccaga cgaagaccag ggccacgacg aagaagccca
     2341 gcgccagcgc gtggctgaat ccagccccgc gctcggccgt cagcgtcacc gccagctcca
     2401 cggcgagcag cccgaacagc gtcgtgaact tgatgacggg gttcatggcc accgacgagg
     2461 tgtccttgaa gggatcaccg acggtgtcgc ccaccacggt ggcggcgtgc agggccgagc
     2521 ccttctcctt cagctccacc tcgacgatct tcttggcgtt gtcccaggcg ccgccggcgt
     2581 tggccatgaa gacggcctgg tagagcccga agagcgcgat ggagatcagg tacccgatga
     2641 agaagtaggg ctcgaagaac gcgaaggcca gcgtcgagca gaagatggtg acgaagatgt
     2701 tgaacatccc cttctgcgcg tactgggtgc agatctccac cacccgcttg ctgtcgtcca
     2761 ccgacgcctt ggtcgagccc tcgagccgga tgttggcctt gatgaactcg acggcccggt
     2821 acgctcccgt gctcaccgcc tgtatggagg cgcccgtgaa ccagtagatg acggccccgc
     2881 cggtcacgag cccgaggagg aagggcgggt gcaacagcga gagcttgtcg aggttctggg
     2941 tgagcccggc cgtcagcagc acgatgatcg agaagatcat ggtggtggag ccgacgaccg
     3001 cggtgccgat cagcacgggc ttggcggtgg ccttgaacgt gttgccggcg ccgtcgttct
     3061 cctcgagcaa gtgcttggca cgctcccagt tgacctcgaa gccgtgatcg cgccggatct
     3121 ccgcggtcac gttcgggatc gtctcgatca cgctcagctc gaagaccgac tgcgcgttgt
     3181 ccgtgaccgg gccgtaggag tccacggcga tggtcacggg ccccatgccg aggaagccga
     3241 aggccaccag gccgaaggcg aagacggccg gcgcgaccat gagggcgccg aggccggccg
     3301 tgctcaccca ccacgcggtg cccatgagtg ccgcgatcac catgccgagc cagtacgcgc
     3361 tgaagttgcc cgccacgagg ccggacagga tggccagcga ggcgccaccc tcccgggcgc
     3421 acgtcaccac ctcgcgcacg tgctccgact ccgtcgacgt gaagatcttg accagctcgg
     3481 ggatgatggc gccggcgagc gtgccgcacg tgatgaccgt ggagagcttc caccagagcg
     3541 agccgtcacc gagatcgctg atgagcgtgt acgaggcgac gtaggtgagc accaccgaga
     3601 ccgccgatgt cagccacacg agggtcgtca gcggggcctc gaagttcatg cgcacggccc
     3661 cggcgtagcg ggcccgcgcg atgacgtcgt tgaggaggta cgaggcgccg ctggcgacga
     3721 tcatgagtac gcgcatcatg aagatccaca cgagcagctg cacctgcacg gccggctcgc
     3781 ggaccgcgag caggatgaac gagatcaggg cgacgccggt cacgccgtac gtctcgaatc
     3841 cgtcggcgct ggggcccacc gagtcgccgg cgttgtcgcc cgtgcagtcg gcgatgacgc
     3901 cggggttgcg ggcgtcgtcc tccttgatgt tgaagacgat cttcatcaga tcggagccga
     3961 tgtccgcgat cttcgtgaag atgccccccg cgatgcggag cgccgccgcg cccagtgact
     4021 cgccgatggc gaagccgatg aagcaggggc ccgcgtagtc gccggggatg aagagcagga
     4081 tgcagagcat caggaacagc tcgacgctga tgagcaacat cccgatggac atccccgcct
     4141 tgagggggat cgcgtagacc gggaagggct cgcccttgag gctcgcgaag gccgtgcgcg
     4201 agttcgcgaa ggtgttgatg cggatgccga accaggcgac accgtagctg ccggcgatgc
     4261 ccacgaggct gaagagcagg atgacgatca cgcgcgcgag gtcgaggtgc agcagcgcgt
     4321 agaagtacgt gaagatgatg acgccgatga acacctcgag cagcaggatg aacttgccct
     4381 gggtgatcag gtaggttttg caggtctcgt agatcagctc cgagatctcg cgcatggagg
     4441 cgtgcaccgg cagctgccgc acctgggagt acatcgtcag cccgaagacg aatcccagca
     4501 cgcacacgcc caggccggcc cagagcagcg tccggccatt catgccgccg accggcacct
     4561 ggccgaggtc gggaatcacg aggctcgcct cgccgccacc ggcgtggggc tgcggcgacg
     4621 gggccgcgtc gacgggaccc ggcccgatgg ccatgagccc gagggcgagg gaggcaagca
     4681 cgaggcaaag gacgagcggc caagcgacgc cgctggacgt ggagcgcatc gggatcactc
     4741 tcctaccgag gaatcgggcc cagccaccga aaagcccagg ccaaatctac gcgacgggtc
     4801 gtgtggtgtc aaagggaaaa gtcttccgat tgccccgaaa gtacgcgagt tgacacgctt
     4861 tgtcgacggt gagtaggatt gccgtcgtca tgtcggacgc ctcgccgcgc gaccgcggcg
     4921 cgagttgtcc cgcctgcggt cggcgcgtgc cgtggccggg gaatccccat cggccgttct
     4981 gttccctcac gtgccgcctc gtggacctcg gtgtgtggct ggacgaaggc taccgcatcg
     5041 cggcggaacc gttcgccgca gaaggagacc gggatgttcc gtagagaggt ccgcacgatc
     5101 atctgcccgt cgtgcggccg tctcacacgt gaagacgcgg cggagtgcct cgtctgcggt
     5161 cgccgtcgtc cgggactgtg gggactcacc tcgctgttcc ggtccgcgtt ccgctctggt
     5221 agcgtcacgt cggtcgtcac ggtcgtgtgc gtcgccgtct acctcgcgag cctggtcctc
     5281 gatccccgtg gggcccttcg atcacgcggc ctcctgggct tcctgccaat cggcgacgcc
     5341 gcggcggccc tgttcgcggc cggcgcgatc ccgtggctcg aaggtcgctg gtggacgctg
     5401 ctgacggcga tctacctcca cggcaacctg ctccacatcc tgttcaacgt gttgtggatc
     5461 cggcagctcg gtccggcggt cgagcagctc tacggccccg cgcgattcat cacgatcttc
     5521 acggtgtccg gcgtcacggg cttcgtcgcc tcgagtctct tggggcacgc ctacacgctc
     5581 ggcgcgtccg ggtcgatctt cggtttgctg ggggcgatcg tcgcgtacgg ccagcgtcgg
     5641 ggcggcgtct tcggcaaggc ggtgctgcgc gagtacggta tgttcgcgct gatcctgttc
     5701 gtcatggcgt tcgtgatgcc cggcgtcgat cacttcgcgc acgccggcgg cttcgtgggc
     5761 ggcctcgccg cgggctacgt gctgtcgatc gccgaccggc gcggggagac gacgctcgac
     5821 catctgctcg ccggcgccgc cgtcgtcctc accgtcatcg ccttcgcgct cgcgctctgg
     5881 agcggcttca cgtcgtagcg acgcgccccg accgaacgac aacgggagtc cgcgcggcgc
     5941 gatcgccctc ggcccggctg gggtgagatc gatcgatcga cagcaaaacg cccgggccga
     6001 ttacttcgac ccgggccgct cgtccgcatc cgacttctgg agaatgctcg aaggggccgc
     6061 cgacggcccc ttcgaggaaa ttaccccggc ggcgacctac tctcccacac cgttgccagc
     6121 gcagtaccat cggccctgga gggcttaact tccgtgttcg ggatgggaac gggtgtggcc
     6181 cctccggcaa cgccaccggg acatcccggg cgcgcatc
//
LOCUS       DATIAZ010000204        11635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1238348, whole genome shotgun sequence.
ACCESSION   DATIAZ010000204 DATIAZ010000000
VERSION     DATIAZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238348"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VLF19_07270"
     CDS             complement(<1..518)
                     /locus_tag="VLF19_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HSE93089.1"
                     /translation="MKLKIGAATIAIERGDITDRDVDAVVNAANTTLAMGTGVAGAIK
                     RKGGLIVEEEAMRQGPVEVGEAVLTTGGNMMATHVIHGAVMGPDLKTDPEKIAATTRS
                     VLAMADKHRITSIALPALGTGVGHVAPPASAEAMLGEVVNHLKKGQSSLRRVVFVLYQ
                     DDAYKAFSETLKR"
     gene            complement(520..1317)
                     /gene="mazG"
                     /locus_tag="VLF19_07275"
     CDS             complement(520..1317)
                     /gene="mazG"
                     /locus_tag="VLF19_07275"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HSE93090.1"
                     /translation="MTESAGRLFERLVDIMARLRSPEGCPWDREQTPTSLKAYLIEEA
                     YEVVEALEDGDSGHLREELGDLLLQVVFHCQIAAEGRAFDVTGVLDRLITKLVARHPH
                     VFGGAPVATPKEALTQWERLKQSEALARGRRRSVIEGVPRALPALLRAQRIQTKAARV
                     GFDWPDAPAAWEKVREEVGETSAAIAEGDRTRVAEELGDLLFSVVNVARLAGLDAEDT
                     LGGAIEKFRRRFTEMEENLSARGTSVEAVSAEELERSWKATKRRERA"
     gene            complement(1369..2031)
                     /locus_tag="VLF19_07280"
     CDS             complement(1369..2031)
                     /locus_tag="VLF19_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93091.1"
                     /translation="MRLGCAGCLSVVLVMALGAGALWSMAGLFQEPDTPPVAASQEDW
                     VTAQQKLLDLLRRSRGRQAQAVTLSEREVTAVVSRQLTQTSSLPLSGTSVRLLAGGTA
                     EVVGRLPLRVAIAHVPLASALEWLPGGWSRRPVWMIIEVRPRLQSKDAGRRYLTLDVE
                     RFAVGRQRLPGVTPRLLLSTASLRYFNVPVPDVVEDVSIERGRLLLRTGTIPPGRPSP
                     PT"
     gene            2316..2705
                     /locus_tag="VLF19_07285"
     CDS             2316..2705
                     /locus_tag="VLF19_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSE93092.1"
                     /translation="MNRLFDGFFGRPATLATGERMWLPLADMYETKDDVYVTFELPGV
                     REKDVDVSITGEVLTVKGERKWERDLKDEAFHRLERVYGKFERSVPLPVPVQGDKVTA
                     AFRDGVLEIKLPKAEEVKPKAIKIDVL"
     gene            2814..4742
                     /gene="dnaK"
                     /locus_tag="VLF19_07290"
     CDS             2814..4742
                     /gene="dnaK"
                     /locus_tag="VLF19_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HSE93093.1"
                     /translation="MAKVIGIDLGTTNSVVAVVEGGNPTVIANQEGSRLTPSVVGFTK
                     DGEILVGQVAKRQAITNPENTVFSIKRFMGRRYDEVLGEIKLVPYKVAKAANGDARVE
                     IRGKQYAPPEVSAMILRKLKEAAEAYLGEKVTQAVITVPAYFNDSQRQATKDAGKIAG
                     LEVLRIINEPTAAALAYGMDKKKDETIAVYDLGGGTFDISILEIGEGVFEVKATNGDT
                     HLGGDDFDQRVIDWIADEFRKEHGIDLRRDRMALQRLKEAAEKAKCELSTTLQTEINL
                     PFITADASGPKHLVLTLTRAKLESLVAELVERTLGPCRQALKDAGVTAKDIDEVILVG
                     GQTRMPKVQDAVKQLFEREPHKGVNPDEVVAVGAAVQAAVLVGEVKDVVLLDVTPLSL
                     GIETLGGVMTVLISRNTTIPTKRSETFTTAADNQESVEVHVLQGERQMARDNRTLGKF
                     HLMGIPPAPRGLPQIEVTFDVDANSILNVSARDKATGKQQAITITAASTLTKDDVERM
                     VKEAETHAAEDAKRKQEIETRNQADSLVYSTERTLSEHGAKLAEADRKAVEEALGEAK
                     EALKSDDVERIQRAQESLMKASHKLAEIMYRESQAQPTGGAEPTGGDQRPAGAPGGQG
                     PKEGEVVDAEFEDLGEKK"
     gene            4753..5634
                     /locus_tag="VLF19_07295"
     CDS             4753..5634
                     /locus_tag="VLF19_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSE93094.1"
                     /translation="MRRDYYAVLGIASTAPPREIRQAYRRLARQYSPDVNFWDEHAQG
                     LFEEIAEAYRVLGDPAARAIYDRFGPPGPGALGPGRRGEDVHISVDLTFGDAARGVAL
                     RLEVPRFSPCAGCSATGTDGETPCRPCHGRGVRRAIDLVEVAIPAGVDTGTQFRVRHE
                     GHAGPFGGPRGDLVVSTRVQEHPFFKREGEGVGCEVPISVWEALRGARVQVPTLTGEA
                     VLVVPPGTPGGQVFRLRGQGLPRLSGDGVGDLYVRIRLEVPAGLDARTDDLVRELERL
                     VPLAPRAGLDRYAGGAQ"
     gene            5631..6017
                     /locus_tag="VLF19_07300"
     CDS             5631..6017
                     /locus_tag="VLF19_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSE93095.1"
                     /translation="MTDGARPLYMIGVVADMLKVHPQTLRFYEKKGLLRPSRTEGRTR
                     MYSQNDVEDLARLIRLTRDLGVNLAGVEIILKMRRQMVEMQKQIEDLLNYLHEQGTEP
                     RGADGREPREALVRAASGHLRGVDLF"
     gene            6053..8428
                     /gene="lon"
                     /locus_tag="VLF19_07305"
     CDS             6053..8428
                     /gene="lon"
                     /locus_tag="VLF19_07305"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSE93096.1"
                     /translation="MDQPVPDILSILPLRSAVVFPHAVVPLAAGRPSSVQLIEDALQR
                     GRLVGTVMQKDPSEDAPGAGGLHAVGTLAMIHRALKQPDGTLRLVVQGVGRFRVVEVL
                     ETAPFLRARVELLGDQDPAAEDVEAEALARSVTSLFQRAVALTPTLPDELAAVADASE
                     GRGALADTVAAALPSLPLALRQELLETVDVKERLQKLAAALGKEVEVLELGSKIQSDV
                     QSEMSKTQREYYLREQLKAIQKELGDGDERTQEIDALREKIDAAGMPEEARKEALREL
                     DRLSKMPPAAAEYTVARTYIDWLVTMPWTAETAEAIDIARAREILDEDHEGLEKIKDR
                     ILEYLAVKKIRPAGKDPILCFVGPPGVGKTSLGRSIARAMGRKFHRISLGGIRDEAEI
                     RGHRRTYIGALPGQIVQGLRRAGTRNPVFMLDEIDKLGMDFRGDPASALLEVLDPEQN
                     GTFRDHYLDVPFDLSRILFITTANVIDTVPAPLRDRMEVIHLAGYTEEEKVRIAQTHL
                     VPKQAREHGLEPKRDVEFTPEALRLIARGYTREAGVRSLEREIAGVCRKVARQRAEGR
                     TEAAIVTPDLVRAFLGAPRFEFEELEERTRVPGVAVGLAWTPAGGDLLFIEATRMKGK
                     GTLALTGQLGDVMKESGQTAMSWVRAHARELGVSPDFWETSDVHIHVPAGAIPKDGPS
                     AGVTLTVALASLLTAQRVRPDLAMTGEVSLSGRVLPVGGIKEKVLAAHRAGVRTVVLP
                     RRNEKNLVEDVPPAVRDVMTFHLVDSVMEALELSLERGVRSEVPRTPAGVA"
     gene            8527..9183
                     /locus_tag="VLF19_07310"
     CDS             8527..9183
                     /locus_tag="VLF19_07310"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSE93097.1"
                     /translation="MALALTLTAAGLAVVPPMAEAQTYRLVSADGTVHLTNTPTHPRY
                     RREGLASGVPGRVPSVALAAVVPFSREIVSAAERHGVPEQLIRAVIRVESGYNPRAVS
                     RKGARGLMQLMPDTASILGVRDLFNPRENIDGGVRHLRNLIGRFGSDLRLALAAYNAG
                     EQAVLMYGGVPPYQETRDYVSRVLALVGVAPELPRSTFQVFERNGTIIYTNIPPRGKL
                     "
     gene            complement(9201..9626)
                     /locus_tag="VLF19_07315"
     CDS             complement(9201..9626)
                     /locus_tag="VLF19_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93098.1"
                     /translation="MSRYFDLRTTILIVVGHGILPEEEDRPIAYELKRVVNGRAGGCE
                     GRAAVVVTDVWLMNNELGDFFPAIALGGPGVNEFTAQIYEDLPVAWTRNERLFIQMAP
                     EGKRAAVWGMDQASTRGAAESFVSEGLLEKFLELVWRQP"
     gene            complement(9623..9787)
                     /locus_tag="VLF19_07320"
     CDS             complement(9623..9787)
                     /locus_tag="VLF19_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93099.1"
                     /translation="MLAHALVLVDSSIAPGRRDEAFAKVTIVLAAAQRLGTLIRARPA
                     SRRDSRIVGE"
     gene            complement(9933..11453)
                     /locus_tag="VLF19_07325"
     CDS             complement(9933..11453)
                     /locus_tag="VLF19_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSE93100.1"
                     /translation="MLARVLSAALVGVEAVLVRVEVDVASGLPAFTTVGLPDSAVRES
                     RERVRTAIRNAGFAFPADRITVNLAPADIRKEGASFDLPIALAILAATGAVTERRPDP
                     LAVVGELALDGAIQPVRGVLAVAIACRRHRIPSLLVPVGNCAEALAVGGMPVLAAATL
                     RDAVSLLNGEGASTPATAPAATPDADAADDADLSDVRGQAYAKRALEIAAGGGHNVLL
                     VGPPGAGKTMLARRLTTILPPLTDDETLEVSTIWSVAGLLPPRGGLVRARPFRAPHHT
                     ISVAGLIGGGNPPRPGEVTLAHRGVLFMDEMPEFAAHVLESLRQPLEDGRVAIARGGG
                     VGDFPSRLQLVAAANPCRRGCASLDACVCSPAERARYLGRISRPLLDRIDLHVELPAV
                     PPGAFAEATGEGSAAVRERVLRARRRQHERLRGGGTRVNANMTARQLRRLCPPSPEAE
                     RLLTAAMTRLGLSARGHDRVLRVARTIADLEDSDAVRPEHCAEAIQYRGLDRQWRG"
BASE COUNT         1865 a         3951 c         4175 g         1644 t
ORIGIN      
        1 cgcttcaatg tctcagaaaa tgccttgtat gcatcgtcct ggtacaggac gaagacgacc
       61 cggcggaggc tggactggcc tttcttgagg tggttcacca cctccccgag catggcctcg
      121 gccgaggcgg gcggcgccac gtggccgacc cccgtgccga gtgcgggcag ggcgatcgag
      181 gtgatgcggt gcttgtccgc catcgccagg accgaccgcg tggtcgcggc gatcttctcg
      241 gggtcggtct tgaggtcggg gcccatgacg gcgccgtgga tgacgtgggt ggccatcatg
      301 ttgccgccgg tcgtcagcac cgcttcaccg acctccaccg gcccctggcg catggcctcc
      361 tcctcgacga tgaggccgcc cttgcgcttg atggcgcccg ccaccccggt gcccatcgcg
      421 agcgtcgtgt tggcggcgtt gacgacggcg tccacgtcac ggtcggtgat gtcccctcgc
      481 tcgatcgcga tggtcgccgc gccgatcttg agcttcatct caggcacgct cccgtcgctt
      541 ggtggctttc cacgagcgct cgagctcctc cgccgagacg gcctcgaccg acgtaccgcg
      601 cgcgctcaag ttctcctcca tctccgtgaa gcggcgccgg aacttctcga tggcgccccc
      661 gagggtgtcc tcggcatcga ggcccgccag gcgcgcgacg ttgaccacgg agaacaacag
      721 atcgcccagc tcctcggcga cccgggtgcg atctccctcg gcgatcgcgg ccgacgtctc
      781 acccacctcc tcgcgcacct tctcccaggc cgcgggggcg tcgggccagt cgaatccgac
      841 ccgcgcggcc ttggtctgga tgcgctgcgc gcggagcaac gcggggagcg ctcggggtac
      901 cccctcgatg acggatcgcc ggcgaccccg cgcgagcgcc tcactttgct tgagccgctc
      961 ccactgcgtc agcgcctctt tcggcgtggc cacaggggcg cccccgaaca cgtgcggatg
     1021 acgtgccacg agcttggtga tgaggcgatc gagcacgcca gtgacgtcga aggcgcggcc
     1081 ctcggcagcg atctggcagt ggaagacgac ctggagcagc aggtcgccca gctcctcccg
     1141 aagatggccg ctgtcgccat cctcgagtgc ttcgacgacc tcgtacgcct cttcgatcag
     1201 ataggccttg agcgacgtcg gcgtctgctc gcggtcccag gggcagcctt cagggctacg
     1261 caagcgcgcc atgatgtcga cgagacgttc gaagagtcta ccggcggact cggtcatgtc
     1321 cgatacctga agcgtgaaag gattatacag gaggtatttg cgggtgtgct aggttggcgg
     1381 cgatggccga ccggggggaa tggtcccggt cctgagcagg agcctgccgc gctcgatcga
     1441 cacgtcctcg accacgtcgg gcacgggcac gttgaagtag cgaagagagg ccgtcgacag
     1501 gagcagccgc ggcgtcacgc ccggaagccg ctggcggccg acggcgaacc gttcgacgtc
     1561 gagggtcaga taacgccgac cagcatcctt gctctgaagg cgtgggcgga cctcgatgat
     1621 catccagaca ggccgccggc tccagccccc cgggagccac tcgagcgcag aggcgagcgg
     1681 tacgtgggcg atggccaccc tcagcggcag ccgacccacg acctcggcag tgccgccggc
     1741 cagcaggcga acgctcgtcc cggacagagg cagtgaggaa gtctgggtga gttggcgtga
     1801 cacgacggcc gtcacctcac gctcgctcag ggtgaccgct tgcgcctggc gaccgcggct
     1861 tcgtcggagg agatccagca gcttctgttg agctgtcacc cagtcttcct ggcttgccgc
     1921 gaccggggga gtgtcgggct cctggaaaag gccggccatg ctccagagcg ctccagcgcc
     1981 cagggccatg accagaacga cgctcaggca gccggcgcag ccgagtcgca tgggcaacaa
     2041 tactactcgc ctgcctcggt cggctgcctt ctaagtcccg aatccgtaag cctatgcgag
     2101 ctatcccggc gggcccggcg atatccctcc gccggataac ttgacagcca tatactcaga
     2161 tgatataaga acaacgtgct cataagatgc tggctaggga ggccggcaag aacatgaggg
     2221 aggaccgtga catggcgatc gatcgttggc gtccttttgg tgcatccgtt tctcggtggg
     2281 agccattccg cgacctgacg gacatccagc acgaagtgaa tcggctcttc gatggcttct
     2341 tcggtcgtcc agcgaccctg gcgaccggcg agcgcatgtg gctgccgctg gccgacatgt
     2401 acgagaccaa ggatgacgtc tacgtgacct tcgagcttcc gggcgtccgc gagaaggacg
     2461 tcgacgtgtc catcacgggt gaagtgctca ccgtgaaggg cgagcgcaag tgggagcgcg
     2521 acctgaagga tgaagccttc catcggctcg agcgggtgta cgggaagttc gagcgctcgg
     2581 tccccctgcc ggtgcccgtg cagggcgaca aggtgacggc cgccttccgc gacggcgtcc
     2641 tcgagatcaa gctgcccaag gccgaagagg tcaagcccaa ggcgatcaag atcgacgtgt
     2701 tgtaaacgcc cggtgtggcg tggttcgagc gccggcacgg ccggcgctcg gcgcgccggg
     2761 gaggtctcgg agggggctgt tcaggccccc tccgaccaca agggagacag gacatggcaa
     2821 aggtgatcgg catcgatctc ggcacgacga actccgtcgt cgccgtcgtg gaaggcggca
     2881 accccaccgt gatcgccaat caggagggca gtcggctgac tccatcggtg gtcggcttca
     2941 ccaaggacgg cgagatcctc gtcggccagg tagccaagcg gcaggcgatc acgaatcccg
     3001 agaacaccgt gttctccatc aagcggttca tggggcgccg ctacgacgag gtgctgggcg
     3061 agatcaagct cgtcccctac aaggtcgcca aggcggccaa cggcgacgcc cgcgtcgaga
     3121 tccgcggcaa gcagtacgca ccgccggaag tgtccgccat gatcctgcgc aagctgaagg
     3181 aggcggccga ggcgtacctg ggcgagaagg tgacgcaggc cgtcatcacg gtgccggcgt
     3241 acttcaacga cagccagcga caggccacga aggacgcggg caagatcgcg ggcctcgaag
     3301 tgctgcgcat catcaacgag ccgacggcgg ccgcgctggc ctacgggatg gacaagaaga
     3361 aggacgagac catcgccgtc tacgacctcg gcggtggcac gttcgacatc tcgatcctcg
     3421 agatcggcga gggcgtcttc gaggtgaagg ccacgaacgg cgacacccac ctgggcggcg
     3481 acgatttcga ccagcgcgtc atcgactgga tcgccgacga gttccggaag gagcacggca
     3541 tcgacctgcg ccgtgaccgc atggcgctgc agcgtctcaa ggaggccgcc gagaaggcca
     3601 agtgcgagct gtccacgacc ctgcagacgg agatcaacct gccgttcatc acggccgacg
     3661 ccagcgggcc caagcacctg gtgctgacgc tcacgcgagc aaagctcgag tcgctcgtgg
     3721 ccgagctggt ggagcgcacg ctcggcccct gccggcaggc gctgaaggac gccggcgtca
     3781 ccgccaagga catcgacgag gtcatcctgg tcggtggcca gacccgcatg cccaaggtgc
     3841 aggacgcggt caagcagctc ttcgagcggg agccgcacaa gggcgtgaat cccgacgagg
     3901 tggtcgccgt cggcgccgcc gtgcaggcgg ccgtgctcgt cggcgaggtg aaggacgtcg
     3961 tgctgctcga cgtgacgccg ctgtcgctcg gcatcgagac gctcggtggc gtgatgacgg
     4021 tgctcatctc gcgcaacacc acgatcccga cgaagcgcag cgagaccttc acgacggcgg
     4081 ccgacaatca ggagtcggtc gaggtgcacg tgctgcaggg ggagcggcag atggcgcggg
     4141 acaaccgcac gctcggcaag ttccacctga tgggcattcc gccggcgccg cgcggtctgc
     4201 cgcagatcga ggtgacgttc gacgtggatg cgaacagcat cctcaacgta tcggctcgcg
     4261 acaaggcgac gggcaagcag caggcgatca ccatcacggc ggcctccacg ctcacgaagg
     4321 acgacgtcga gcgcatggtg aaggaggccg agacccacgc ggcagaggac gccaagcgca
     4381 agcaggagat cgagacgcgc aaccaggcgg actcgctcgt gtactcgacg gagcgcacgc
     4441 tgtcggagca cggcgccaag ctggccgagg cggaccgcaa ggcggtcgag gaggccctcg
     4501 gggaggcgaa agaggcgctg aagtccgacg acgtcgagcg catccagcgc gcccaggaga
     4561 gcctcatgaa ggcctcgcac aagctcgcgg agatcatgta ccgcgagagc caggcccagc
     4621 cgacgggcgg cgcggagccg accggcggcg accagcgccc cgcgggcgcc ccgggcggtc
     4681 aggggccgaa ggagggcgag gtggtggacg ccgagttcga ggacctcggc gagaagaagt
     4741 agaggcggtc cgatgcggcg cgactactac gcggtgctgg ggatcgcctc gaccgcgccg
     4801 ccgcgcgaga tccgccaggc gtaccggcgg ctcgcgcggc agtactcgcc cgacgtgaac
     4861 ttctgggacg agcacgcgca gggcctcttc gaggagatcg ccgaggcgta ccgcgtcctc
     4921 ggcgatcccg ccgcgcgcgc gatctacgac cgcttcgggc cgcccggacc cggtgcgctg
     4981 ggccccggcc ggcgcggcga ggacgtgcac atctcggtgg acctcacctt cggtgacgcg
     5041 gctcggggcg tggccctccg gctcgaggtg cctcgtttct cgccgtgcgc tgggtgctcg
     5101 gcgaccggga ccgacggcga gacgccgtgt cggccgtgcc acggacgtgg cgtccgccgg
     5161 gcgatcgacc tcgtggaggt ggccattccc gccggagtgg ataccggcac gcagttccgg
     5221 gtgcgccacg aaggtcacgc gggcccgttc ggcggaccgc gcggggacct cgtggtgagc
     5281 acgcgcgtgc aggagcatcc cttcttcaag cgcgaggggg agggcgtcgg ctgtgaggtg
     5341 cccatcagcg tgtgggaggc cttgcgcggt gcgcgcgtgc aagtgccgac gctcacgggc
     5401 gaggcggttc tggtcgtgcc gcccggcaca ccgggcgggc aggtcttccg cctgcgcggt
     5461 cagggactgc cgcgtctgag cggggacggc gtgggagacc tctacgtgcg gatccggctc
     5521 gaggtgccgg cgggcctgga cgcccggacc gacgacctcg tgcgcgagct ggagcgtctg
     5581 gtgccgctgg ccccccgcgc cggcctcgat cgatatgcgg gaggcgcgca gtgaccgacg
     5641 gggccaggcc gttgtacatg atcggcgtgg tcgccgacat gctgaaggtg cacccccaga
     5701 cactgcggtt ctacgagaag aaggggctgc tgcgcccgag ccggaccgag gggcggacgc
     5761 ggatgtactc gcagaacgac gtcgaagatc tggcgagact gatccgtctc acgcgtgatc
     5821 tgggcgtgaa tctcgccggc gtcgagatca tcctaaagat gcggcggcaa atggtcgaaa
     5881 tgcagaagca gatcgaggac ctcctgaact acctgcacga gcagggcacc gagccgcgcg
     5941 gcgcggacgg ccgcgaaccg cgtgaggcgc tggtccgcgc ggcctcgggg catctccggg
     6001 gcgtcgatct cttctgaggc gacgagaaag gcaaggtgac cccgtgacgg tcatggacca
     6061 gccggtcccc gacatcctct cgatccttcc gctgcgctcg gcggtggtct tcccccacgc
     6121 ggtggtgccg ctggccgcgg gccgcccgtc ctcggtgcag ctcatcgagg acgccctgca
     6181 gcgcggacgg ctcgtgggca cggtcatgca gaaggatccg agcgaggacg cgcccggcgc
     6241 cggcggtctc catgccgtgg gcaccctcgc gatgatccac cgcgcgctca agcaacccga
     6301 cggcacgctg cgcctcgtcg tgcagggcgt cggccgtttc cgggtggtcg aggtgctgga
     6361 gaccgccccg ttcctccgcg cccgcgtgga gctgctcggc gaccaggatc cggccgccga
     6421 ggacgtcgag gcggaggcgc tcgcgcgcag cgtgacgagc ctgttccaga gggccgtcgc
     6481 gctcacaccg acgctgcccg acgagctcgc cgccgtggcc gacgcctcgg aggggcgggg
     6541 ggcgctggcc gacaccgtgg cggcggcgct gccgagcctg ccgctcgcgc tgcgccagga
     6601 gctgctcgag accgtggacg tcaaggagcg gctgcagaag ctggccgcgg cgctcggcaa
     6661 ggaagtcgag gtcctcgagc tgggctcgaa gatccagtcg gacgtccagt ccgagatgtc
     6721 caagacccag cgcgagtact acctccgcga gcagctcaag gccatccaga aggagctcgg
     6781 cgacggcgac gagcgcacgc aggaaatcga tgccctccgc gagaagatcg acgcggcggg
     6841 catgcccgag gaggcgcgca aggaggcgct ccgcgagctc gaccgcctgt ccaagatgcc
     6901 gccggcggcg gccgagtaca cggtcgcccg cacgtacatc gactggctcg tgacgatgcc
     6961 gtggaccgcg gagaccgcgg aggccatcga catcgcgcgc gcccgggaga tcctcgacga
     7021 ggaccacgag ggcctcgaga agatcaagga ccggatcctc gagtacctgg cggtgaagaa
     7081 gatccggccg gccggcaagg acccgatcct ctgcttcgtg ggtcctccgg gtgtcggcaa
     7141 gacgtcgctg gggcgctcca tcgcgcgcgc aatgggccgg aagttccacc gcatctcgct
     7201 cggcggaatc cgggacgagg cggagatccg cggtcatcgt cgcacgtaca tcggcgcgct
     7261 gccgggacag atcgtccagg gcctgcgccg cgccggaacg aggaacccgg tcttcatgct
     7321 cgacgagatc gacaagctcg gcatggactt ccgcggtgat cccgcctcgg cgctgctgga
     7381 ggtgctcgat cccgagcaga acggcacatt ccgtgaccac tacctcgacg tgccgttcga
     7441 tctctcacgc atcctcttca tcacgacggc gaacgtgatc gacaccgtgc ccgcgccgct
     7501 gcgcgaccgc atggaggtca ttcacctcgc cgggtatacc gaggaggaga aggtccggat
     7561 cgcgcagacc catctcgtgc ccaagcaggc acgcgagcac ggtctcgagc cgaaacgcga
     7621 cgtggagttc acgcccgagg cgctgcgcct gatcgcgcgc ggctatacgc gcgaggccgg
     7681 cgtgcgcagc ctcgagcgcg agatcgccgg cgtctgtcgc aaggtggccc ggcagcgggc
     7741 ggagggacgc acggaggcgg cgatcgtcac cccggacctc gtgcgcgcgt tcctgggagc
     7801 gccacgcttc gagttcgagg agctggaaga gcgcacgcgg gtccccggtg tggccgtcgg
     7861 tctggcctgg acacccgcgg ggggcgatct cctgttcatc gaggccacgc gcatgaaggg
     7921 caagggcacg ctggccctga cgggccagct cggcgacgtg atgaaggagt ccgggcagac
     7981 cgccatgtcg tgggtccgcg cccacgcgcg agagctcggc gtgtcgcccg atttctggga
     8041 gacctccgac gtccacatcc acgtgccggc cggggccatc ccgaaagacg ggccgtcggc
     8101 cggggtgacg ctcacggtcg ctctcgcctc gctgctcacg gcgcagcgcg tgcggcccga
     8161 cctggcaatg accggcgagg tgagcctgtc cggacgcgtg ctgcccgtcg gcggcatcaa
     8221 ggagaaggtc ctggccgctc accgggccgg ggtgcgtacg gtcgtcctgc ctcggcgcaa
     8281 cgagaagaac ctcgtcgaag acgtaccgcc ggcggtccgc gatgtcatga cgtttcatct
     8341 cgtcgacagc gtgatggagg cgctcgagct gtcgctggag agaggcgttc ggtcggaggt
     8401 cccgaggact ccggccgggg tggcctgacc tagtattttc ggctcacgcc catcggggcg
     8461 gcgcgggcga cgtccacggc ttgacaccgc ctcgcgctca ccggctatca tgggtgcgtg
     8521 acgctgatgg ccctcgcttt gaccctgacc gctgccgggc tcgccgtcgt ccctccgatg
     8581 gcggaggcgc agacgtatcg cctggtttcg gccgacggca ccgtccacct gaccaacacc
     8641 cccacccatc cgcggtatcg ccgtgagggt ctcgcgtccg gcgttcctgg ccgggtaccg
     8701 tcggttgccc tggcggccgt ggtcccgttc tcccgggaga tcgtctctgc ggccgaacgg
     8761 cacggcgtcc ccgaacagct gatccgtgcc gtcatccgcg tggaatcggg ctacaacccg
     8821 cgcgcggtgt cgcgcaaggg cgcgcggggt ctcatgcagc tcatgccgga cacggcttcg
     8881 atcctcggcg tccgggacct gttcaacccg agggagaaca tcgacggggg cgtgcgccac
     8941 ctgcgcaacc tcatcgggcg cttcgggagc gacctgcgtc tcgcgctggc cgcgtacaac
     9001 gccggcgagc aggccgtgct gatgtacggc ggcgtcccgc cctatcagga gacgcgcgac
     9061 tacgtcagcc gggtgctcgc gctggtgggc gtggcgcccg agcttccgcg ctcgacgttc
     9121 caggttttcg agcgcaacgg gacgatcatc tacacgaaca ttccgccgcg cgggaagctg
     9181 tagcagccgg gctcactcgg ctacggctga cgccagacga gctccaggaa cttctcgagc
     9241 aggccctcgc tgacgaacga ctccgccgcc ccgcgcgtgg acgcctggtc catgccccag
     9301 acggcggcgc gcttgccttc gggcgccatc tggatgaaga gccgctcgtt cctggtccag
     9361 gcgaccggca gatcctcgta gatctgggcc gtgaattcgt tgacgcccgg gccgcccagc
     9421 gcgatggcgg ggaagaaatc gccaagctcg ttgttcatca gccacacgtc cgtcaccacc
     9481 acggcagccc gcccctcgca cccgcccgcg cggccgttca cgacgcgctt gagctcgtag
     9541 gcgatcggcc gatcctcctc ctcgggaagg atcccgtgac cgacgacgat gagaatcgtc
     9601 gtccggaggt cgaagtaccg actcattcac ccacgatccg gctgtcgcgg cgtgatgccg
     9661 gccgcgcccg gatgagagtg cccaggcgct gggcggcagc gagcacgatc gtgaccttcg
     9721 cgaacgcctc gtcgcgtcgc ccgggggcaa tcgaagaatc gacgaggacc agcgcgtgag
     9781 ccagcatggc gcctccgcgg gctgtggcgt cgaaaccatt ctctcatgca ttgacggaca
     9841 agccagccga ccgggccccc ggccgagacc tctccggatc tgcccggggc gtcgagcacg
     9901 agggatggca gcccggaggc cctctcctct cctcaccctc gccactggcg gtcgaggccg
     9961 cggtactgga tggcctcggc gcaatgctcc ggacggacgg cgtcactgtc ctcgaggtcg
    10021 gcgatcgtgc gggccacccg gagtacccgg tcgtgaccgc gcgccgagag tccgagcctg
    10081 gtcatggccg cggtgaggag ccgctccgcc tccggcgagg gcgggcagag acgacggagc
    10141 tggcgcgccg tcatgttggc gttgacacgg gtgccgccgc cccgaaggcg ctcgtgctgc
    10201 cgccggcgcg cgcgcaacac gcgttcacgc acggccgccg agccctcgcc ggtggcctcg
    10261 gcgaacgcac ctgggggcac ggccggcagc tccacgtgca ggtcgatccg gtcaaggagc
    10321 gggcgcgaga tgcggcccag gtaacgcgcg cgctcggcgg gagagcacac gcacgcgtcc
    10381 agggaggcgc acccgcggcg gcaggggttg gcggcggcaa cgagctgcag gcgggaagga
    10441 aagtcgccga cgccgccgcc acgcgcgatg gccacgcggc cgtcctccag cggctggcgg
    10501 agcgactcca gaacgtgcgc ggcgaactcc ggcatctcgt ccatgaacag gacgccgcga
    10561 tgagcgagcg tcacctcgcc cggccgcggg gggttgccgc cgccgatgag gcccgcgacc
    10621 gagatcgtgt ggtggggcgc gcggaagggc cgggcgcgca cgaggccgcc cctcggaggc
    10681 agcaggcccg ccacgctcca gatcgtcgac acctcgagcg tctcatcgtc ggtgagcggg
    10741 ggcaggatgg tggtgaggcg ccgcgccagc atcgtcttcc ccgccccggg agggccgacg
    10801 agcagcacgt tgtgtccgcc gccggcggcg atctcgagtg cgcgcttggc gtaggcttgc
    10861 ccgcgcacgt cggagagatc cgcgtcgtcg gcggcgtccg cgtcgggggt cgccgccggc
    10921 gccgtcgcgg gggtgctggc gccttcgccg ttcagcaggg agaccgcgtc gcgcagcgtg
    10981 gccgcggcga gcaccggcat gccgccgacg gcgagggcct cggcgcagtt gccgacgggg
    11041 acgaggagcg acgggatgcg atggcgccgg caggcgatgg ccacggcgag gacgccgcgg
    11101 accggctgga tggcgccgtc gagcgcgagc tctccgacga cggcgagggg atcgggccgc
    11161 cgctccgtga cggcgccggt ggcggcgagg atggccagcg cgatcgggag gtcgaacgag
    11221 gcgccctcct tgcggatgtc ggcgggcgcc aggttgacgg tgatgcggtc ggccggaaag
    11281 gcgaacccgg cattgcggat ggcagtgcgc acgcgctcgc ggctctcgcg cacggcggaa
    11341 tcgggcagcc ccaccgtcgt gaacgcgggc agcccgctgg cgacgtcgac ctcgactcgc
    11401 acgagcacgg cctcgacgcc gacgagagcc gcggagagca cgcgcgcgag cacgcgagga
    11461 ttcttcggcc gcgccgccga ccggtcaatg ccgtcgattc gataccccgc tccgataccc
    11521 tccgcccgca cttcgcgcgc cgggcccgtt cggtgctaga gtggccgaat catggcgcgc
    11581 gagctgcggg tcgcggaggg gaccgagccg gtccggcacc tgcgcccgac gatcc
//
LOCUS       DATIAZ010000205        16348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1635143, whole genome shotgun sequence.
ACCESSION   DATIAZ010000205 DATIAZ010000000
VERSION     DATIAZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 16348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16348
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635143"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VLF19_07330"
     CDS             <1..610
                     /locus_tag="VLF19_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSE93101.1"
                     /translation="KMLREGVRKAGKQTDVRINKAGCFSQCGHGPMVVVYPENVWYAG
                     VQESDLEEILASHIVGGRPVERRRYEPGVPGPNKIETAHTDAAPGRAPQPEPRPQRST
                     WTRVCAAADVPANGMKEFPVDGVNVLIVNAADAFVAYQALCPHEAVPLEQGIHDGCVL
                     TCLEHMWQFDVKTGAPMGDAQEGLKGYRLKEERGELYVALEG"
     gene            626..1708
                     /locus_tag="VLF19_07335"
     CDS             626..1708
                     /locus_tag="VLF19_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE93102.1"
                     /translation="MTRPALTTVEADFHERMHAARIHGLWELASQMTRHPEPKAVAHM
                     WRSSLLEAMVRESGEVVPVGEERRALQLFNPGLDGRWATTNTMIAAVQLLLPGEVARA
                     HRHTPTAIRFIMEGEGAYTAVDGERVYMAPGDLILTPSWAWHDHGNETDRPVVWMDGL
                     DVPLVQALNAMFFQMYDVAQVPLSKPDNASVQLHGHVSLSPTWVKERSRSSPLLLYPW
                     ATTWPSLNALRAVEGDPHDGVALEYTHPQTGRSLLPTMACWIQMLRPGERLKAHRHTG
                     SAVYYVVQGAGETIIDGCRFAWGRGDILVLPSWALHEHANRSTRDAAVLFSIQDRPVL
                     EALGLYREEAFTESGGHQAVSSSFEA"
     gene            1859..2011
                     /locus_tag="VLF19_07340"
     CDS             1859..2011
                     /locus_tag="VLF19_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93103.1"
                     /translation="MAFCHERLASDKVPARLAFRKADEFPRTPTGKIHKPGIRDELAQ
                     RSPGIE"
     gene            2033..3238
                     /locus_tag="VLF19_07345"
     CDS             2033..3238
                     /locus_tag="VLF19_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE93104.1"
                     /translation="MSRALGGIRVLDLTQFEAGPSCTQLLAWLGAEVIKVEEPGKGDP
                     GRYGRAEKPGVDSSYFLTLNANKKSVTLNLKHAQGKAMFLDMVKQVDVVAENHAPGAI
                     EKMGLGYETLSGVNPRLVFLSVKGFGTYGPYSQYKSFDMIAQATGGAMALTGTPGSPP
                     LKPGPTIGDSGTGVHAALGVMAALWQRQASGRGQRVEVSMQDAVVNFCRVAMRLAYAG
                     GDFEPRRGNEIANTVPSGIYRCKPGGPDDYAYIYPQPVRTHMWDALLRAIGRGDLVGD
                     SRWSDPKWRAAHKDEVNALVESWTMERTKHEVMAVLGAAGVPAGACLTPNEILSDPHL
                     AARRMVVTVDHPGWGPFTMPGNPVQLSGSPTEVRPAPLLGQHNAEIYKEWLSLGAEDL
                     ARLRADGVV"
     gene            complement(3353..4216)
                     /locus_tag="VLF19_07350"
     CDS             complement(3353..4216)
                     /locus_tag="VLF19_07350"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE93105.1"
                     /translation="MSVVLVTGSSSGIGLATVLHFARLGHDVHAGVRNPGTATDLSKA
                     IEVDALPIRPVALDVDDTASVTRAVDEVLRRSGRVDVLVNNAGIGGGGPIADVPVDWA
                     KTLFETNYFGTIRMIQAVLPGMREQRSGAIVNVSSIAGRVAIAGHGHYSAVKHALEAA
                     SEALAQEVVAFGIRVAIIEPGVVVTPIFSKARRFADPASPYAVHVRRLLLFYQMQMKM
                     PSQPEDVARMIHEAVTTDTPRLRYLVGEDARRLAAGRERLTDEEYVATGREMPDAAYL
                     DLMRRRYGFEW"
     gene            4459..4935
                     /locus_tag="VLF19_07355"
     CDS             4459..4935
                     /locus_tag="VLF19_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93106.1"
                     /translation="MTEREALTALRGADPAQAARAEAALWAMWTRSTDSEVNVLMQDG
                     IAAMQARRFADAERLFTRITECAPDFAEGWNKRATVRYLAEDFAGAITDCEATLARNP
                     NHFGALSGQGLCHLARGEAREAAALFRRALAVHPHLGAARENLRLALANAVQGNGH"
     gene            4972..5439
                     /locus_tag="VLF19_07360"
     CDS             4972..5439
                     /locus_tag="VLF19_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HSE93107.1"
                     /translation="MAGALTVETPHDLLSHVGREFGPSEWMTVDQAMIDKFADATGDH
                     QWIHVDVERAKREMPGGKTIAHGYLTLSLVPRLAATLVKVNKVTHGVNYGSNRVRFTA
                     TVPAGSRVRLRLKIAKVEPVEGNGVRVTNEVTMEVEGQDRPALVAEIMGIRYA"
     gene            5471..6493
                     /locus_tag="VLF19_07365"
     CDS             5471..6493
                     /locus_tag="VLF19_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93108.1"
                     /translation="MTDSNDPAELIEAYFDRGWTDGLPVVPPTEKSVAAMLAGAALEG
                     REVIGEISGRNTRVTADKVAINAVLAGCRPEYLPVVVAAVEGLCHPDFAYHGPASSTG
                     GSAMVLIVNGPLGRRLGVNSGNNAFGQGVRANATIGRAVRLVMMNVMQTRPGLLDRAT
                     LGNPGKYAFCFAENEEDHPWEPLHVERGFRPEQSTVTVYASNSLYQVYNQLAAEPEPL
                     LRCFADALGNLGIPNMKGFNQALLVFAGEHSEVFRQSGWTKRRVKEFLVAHTRRPVSD
                     WKRAARLPGAVTPDDETTLRALFEDPDDLLLVCAGGRAGSWSAGLPGWGKKWTRSVTT
                     LIREIS"
     gene            6531..6812
                     /locus_tag="VLF19_07370"
     CDS             6531..6812
                     /locus_tag="VLF19_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93109.1"
                     /translation="MRKLLDPTSSPVARDVAVAARPASLRGLRLALVENTKFNSDALL
                     TRLAERLARDHGLATTVMHRKRSPSHEVEAAAVAELRAKADVVISGIGD"
     gene            6834..7055
                     /locus_tag="VLF19_07375"
     CDS             6834..7055
                     /locus_tag="VLF19_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93110.1"
                     /translation="MLDAILLERAGVPAVAVVTEPFQATGEAMAASWGMAGYRFLTTP
                     HPIANLGDKDLDERAERLVAPVVALLQNA"
     gene            7233..8213
                     /locus_tag="VLF19_07380"
     CDS             7233..8213
                     /locus_tag="VLF19_07380"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HSE93111.1"
                     /translation="MIHTPVCDLLQIRHPIVLGGMAGSTSVPLVAAVSNAGGLGTLGI
                     ARTPAEQIPRDIGAIREATKGPFGVNFLLFVAREPALQAALDARPPVFSAAWARPDQD
                     LRALFGRAHEAGSQVMYMVGTVPEAVRAKAAGADVIVAQGTEGGGHVGWMASMAVLPM
                     IVDAVGPTPVVAAGGIADGRGLAAALALGAQGALLGTRFLATKESPLHLNFKQAVLDS
                     DGHDTVLTEIPDIARANVWPGAMARARRNAFIERWAGREWELRGRQPEAAAALERARE
                     TGDVDNASLLIGQDAGLIHDIPPAGEIVERIVAEAEALLKDRLPKLVRVG"
     gene            complement(8352..9488)
                     /locus_tag="VLF19_07385"
     CDS             complement(8352..9488)
                     /locus_tag="VLF19_07385"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSE93112.1"
                     /translation="MAQRFPLETSTPAALNLDATALGRLDELINRHIAEGRYPGAQVA
                     IAREGKLALVKTYGDARLDPQRVAARDDTLWLLFSNTKVITACAVWLLAERGALRFTD
                     RVAEHLPGFEANGKGDITIVQLLSHRAGFPNADVPKEAWEDHELLRRTVCGFTLEWTP
                     GARLHYHARSAHWTAATMIEAITKTDYRAFIRDNVLVPLGLGDELFVGLPDTEHGRAV
                     DMHEPSADGGRQVKRADENTAPFRRAGAPASGGYATARAMAAFYQMMLAGGRLGGVRL
                     LSPRTVQYVTRNVTGDMVDGYMGMPMHRGLGPHLRGTTDTIRGLGSLASPRTFGHGGV
                     GSSYCWADPDSGVSFAYLTNSRVGDPWHSARLDVISNCVHASID"
     gene            complement(9529..11598)
                     /locus_tag="VLF19_07390"
     CDS             complement(9529..11598)
                     /locus_tag="VLF19_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93113.1"
                     /translation="MEASPGSRVKVLVVDDMPGQRQAIEAALAELGEEIIAVGSGVEA
                     LRVLLDHDVAVILLDVTMPDMDGFETASLVRQRPRSRHTPIIFLTAHMDEMAAARAYS
                     LGAVDYIFNPFAPDVLRAKVRVFVELSRIHERMRREAEHRIALSRAQAARAAAEEESR
                     RLRVLAEASGVLTRSLDTRTLVADLLALFVPLVADLAAISLTDAGGHTAETSWLDARP
                     AARVSRDPVPDDDGLALNARLEGVVAADRAERIVSHDGQHTQGIILPLVARGRTFGAM
                     ASLMRLPRRYSDADLDLVRDIAGRAAIALDNSRLYQEIHERDRQKDEFLAMLSHELRN
                     PLGAITTAVRLLEVIGTADERATRAREVIGRQAGHLARMVDDLLDVARLTVGQIALDR
                     APIDLADVVARALDALRVSGRLEHHAVTVKADSITVDADFDRLEQVVTNLLVNAAKYT
                     DRGGCIHVEVRADGDEAVVRVRDTGIGISAEMLPRLFDLFTQGRQALDRAQGGLGIGL
                     TLVRRLVELHGGSVAAASEGPGRGSVFTVRLPRATREKIDDGGRGPGQGDRAPLRILL
                     VEDSRDTRETLRSLLELAGHEVHEAADGFEAVRVACRVNPQLALIDIGLPGLDGLQVA
                     SRIRDTAEGNAVVLVAVTGYGQLTDRRKALAAGFDLHLTKPVEPERLDEVLALAARRS
                     PRRKVIT"
     gene            11813..12865
                     /locus_tag="VLF19_07395"
     CDS             11813..12865
                     /locus_tag="VLF19_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSE93114.1"
                     /translation="MAMADTTIRVGFVGAGNNTRRRHIPGFQKLAGVELVAVANRTKE
                     SSERVAREFGMRGVYADWRDLVRAPEVDAVCIGTWPNMHREITEAALAAGKHVLCEAR
                     MAMNAAEGRRMLEASRGAPSLVTQLVPSPPTLEVDSTIQALLADGYLGRVQAVELQAT
                     QNAGFVDADAPLHWRQDIALSGHNVLNMGIWYEAVLRWLGPARRVMAMGRVAVPRRRD
                     AGGAWHDVRVPDHIDILATFGEGTVGRLRFSSLTALAPPSEVWIFGSDGTLRLEADAR
                     RLSGGRRGDRELREIAIPAERRVGWRVEEEFVNAIRGREKIARTTFEDGVRYMEFTDA
                     VARSVASGQAVDVAEL"
     gene            12876..13925
                     /locus_tag="VLF19_07400"
     CDS             12876..13925
                     /locus_tag="VLF19_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HSE93115.1"
                     /translation="MGRLAALPPLPTTVVGSYPQPEWLIDRDVLLRTGVPRVGLPGLW
                     RVPATLREQAEDDAVRLAVRDMERAGIDVVSDGEARRESYFNWLAPFLTGIDLERPGT
                     AISRTGKPTPVPRVVGPITRPQPVLARHTAFLRAETDRPIKVTVPGPFTLTALAQNEH
                     YPDAPSLALAFADAVNAELRDLRSAGADFIQLDEPYLQAWPDRAREYGVAVIDRALAG
                     VAGPTVIHLCFGYAHTVGSAKPSGYSFLPELDRCRGTHISIEAAQPRLDCAVLRSLPS
                     KTIVLGVLDLNDPAVETPEIVADRIRKALAHVPSERLVVAPDCGMKYLPRERAGAKLH
                     ALAAGARLVRAELAG"
     gene            complement(14024..15340)
                     /locus_tag="VLF19_07405"
     CDS             complement(14024..15340)
                     /locus_tag="VLF19_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HSE93116.1"
                     /translation="MTLETKYGVPTVALHTDKFDRVVRSVAQVAGMPGLRQAFVPQPV
                     MGKTPRELRAYVDGTDPISGRPVMQEILEGLTRPITDDERGPDGFDRSTPRLLPPATE
                     ESLRQLFLERHWTDGLPIVLPTEARVAAMLAGTRRKPDEPVGRMRSTHTREAWAYSVE
                     KVAVNAVMAGARPEYLPVILALAATGVTARSSSSSAMATMAVVNGPIRHETGMNSGTG
                     AMGPYSQANATIGRAYSLLSQNLQGGSVPGLSYMGNQGNAYAYAGVTFAENEERSPWE
                     PFHCDHGFAPGDSAVSVFTGCRSTAFTLGLRETHWREHVANLLRGMDPTGPPTLVLDP
                     IAARQFVERGGFVEKKAMLDWIHEIARLRAREYWDYQLVQNYIYPRATFGEEPWASRL
                     RAAPDELIPMFRREDVHAVVVGGETNGYWRIMGCTYQKTVSVDDWR"
     gene            complement(15374..15667)
                     /locus_tag="VLF19_07410"
     CDS             complement(15374..15667)
                     /locus_tag="VLF19_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93117.1"
                     /translation="MSERMTVFDPTGLPPVVSRKDLAPRLPTLEGRTVYLVDCRFDDS
                     GLLLEQVQAWLGEHMPGVRTVLKPISSVYLVDDPATWEEIRQRGHAAVLGVGH"
     gene            16004..16231
                     /locus_tag="VLF19_07415"
     CDS             16004..16231
                     /locus_tag="VLF19_07415"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93118.1"
                     /translation="MPLYARTGVLEVWLVNRQTDAIEMFRACEVARLTVALARGTVAR
                     SSRFSRSHAPCRRHPREHAGLSTSGAWPGSP"
BASE COUNT         2366 a         5743 c         5926 g         2313 t
ORIGIN      
        1 gaagatgctc cgggagggcg tgcggaaggc cggcaagcag acggacgtgc ggatcaacaa
       61 ggccggctgc ttctcgcagt gcggccacgg tccgatggtc gtcgtctatc ccgagaacgt
      121 ctggtacgcg ggcgttcaag agtcggacct ggaagagatc ctggcgtccc acatcgtcgg
      181 cgggcggccc gtcgagcggc ggcgctacga gcccggcgtg ccgggaccga acaagatcga
      241 gaccgcgcac acggacgcgg cgcccggccg cgctccccag cccgagccgc gcccgcagcg
      301 atccacgtgg acgcgggtgt gcgcagccgc ggacgtgccg gccaacggca tgaaggagtt
      361 ccctgtcgac ggtgtcaacg tcctgatcgt caatgccgcg gacgcgttcg tcgcctatca
      421 ggcgctgtgc cctcacgagg ccgtgccgct cgagcagggc attcacgatg ggtgcgtcct
      481 gacctgcctc gagcacatgt ggcagttcga cgtcaagacg ggcgcgccga tgggcgacgc
      541 tcaggagggg ctgaagggat atcggctcaa ggaagagcga ggcgagctct acgtcgcgct
      601 cgagggctga ggctcaggag ggaacatgac tcgtccggcg ctcaccacgg tcgaagcaga
      661 ctttcacgag cggatgcacg ccgcccggat ccatggcctc tgggagctgg cgagccagat
      721 gacgcgtcac cccgagccga aggccgtcgc ccacatgtgg aggtcgtcgc tgctcgaggc
      781 catggtccgc gaatccggcg aggtcgtccc ggtgggcgag gagcgtcggg cgctccagct
      841 cttcaacccg ggcctcgacg gccggtgggc cacgacgaac acgatgatcg ccgccgtgca
      901 gctcctgctt cccggagagg tcgcccgcgc gcaccgtcac acgccgacgg cgatccgctt
      961 catcatggag ggcgaaggcg cctacaccgc ggtcgacggc gagcgcgtct acatggcgcc
     1021 cggcgacctc atcctcacgc cgagctgggc gtggcacgac cacggcaacg agaccgacag
     1081 gccggtggtc tggatggacg gcctcgacgt gcccctcgtc caggccctga acgcgatgtt
     1141 cttccagatg tacgacgtgg cccaggtgcc gctgagcaag cccgacaacg cctcggtcca
     1201 gctccacggc cacgtcagcc tgagtccgac ctgggtgaag gagcggtcgc gatcctcgcc
     1261 cctgctcctc tatccctggg cgacgacgtg gccgtcgctc aacgccctgc gcgccgtcga
     1321 gggcgatccg cacgacggcg tcgcgctcga gtacacgcat ccgcagaccg gccgttcact
     1381 gctgccgacg atggcgtgct ggatccagat gcttcgaccc ggcgagcgcc tgaaggcgca
     1441 ccgtcacacg gggagtgccg tctactacgt cgtccagggg gccggggaga cgatcatcga
     1501 cggctgccgc ttcgcctggg gccggggcga catcctcgtc cttccgtcct gggccctgca
     1561 cgagcacgcc aatcgttcca cgcgtgacgc cgccgtgctc ttctcgatcc aggaccggcc
     1621 cgtcctcgag gcgctgggcc tgtaccggga ggaggccttc acggagagcg gcggtcacca
     1681 ggcggtgagc tcgagcttcg aagcctagcg acaggccgtg agccgccttc ccttagaagc
     1741 gccagccgcg ccacgggcga cggcgcccgg gatcggatcc gggtcggtgc ggggtccagc
     1801 gcgccgggcg cgtcgtcacc agggccgcgc accgcgaggg gcggatgccg gagcgctcgt
     1861 ggccttctgc cacgagcggc tcgccagcga caaggtcccg gcgcggctcg cgttccgcaa
     1921 agccgacgag ttcccccgca cgccgacggg caagatccac aagcccggga tccgcgacga
     1981 gctcgcgcaa cgttcccccg gaatcgagta atctcccgcc aggagaccaa ccatgagcag
     2041 agcactcggc ggcattcgcg tcctcgacct gacccagttc gaggccgggc catcgtgcac
     2101 gcagctgctg gcctggctcg gggcggaggt gatcaaggtc gaggagcccg gcaagggcga
     2161 tcccggacgc tatggccgcg ccgagaagcc gggcgtggac agcagctact tcctgacgct
     2221 caacgccaac aagaagagcg tcacgctcaa cctgaagcac gcccagggca aggccatgtt
     2281 cctcgacatg gtcaagcagg ttgacgtcgt cgccgagaac cacgcgcccg gagcgatcga
     2341 gaagatgggc ctgggctacg agacgctgag cggcgtcaat ccccgcctcg tcttcctgtc
     2401 ggtcaagggc ttcggcacct acgggccgta cagccagtac aagagcttcg acatgatcgc
     2461 ccaggccacc ggcggagcga tggcgctgac gggcacgccg ggttctccgc cgctcaagcc
     2521 cggcccgacc atcggcgact cgggcacggg cgtgcacgcg gccctcgggg tgatggcggc
     2581 gctctggcag cgccaggcct cgggccgggg acagcgcgtc gaggtgtcca tgcaggacgc
     2641 ggtcgtcaac ttctgtcgcg tcgccatgcg tctggcctac gccggcggag acttcgagcc
     2701 gcgccgcggc aacgagatcg ccaacaccgt tccgtcgggc atttaccgct gcaagccggg
     2761 cggccccgac gactacgcct acatctaccc ccagccggtc cggacccaca tgtgggacgc
     2821 cctgctgcgc gccatcgggc gcggcgacct cgtcggcgat tcccggtggt ccgatccgaa
     2881 gtggcgggcc gcgcacaagg acgaggtcaa cgcgctcgtc gagagctgga cgatggagcg
     2941 cacgaagcac gaggtgatgg cggtgctggg agccgcgggc gtcccggccg gcgcgtgcct
     3001 gacgccgaac gagatcctga gcgatccgca tctcgcggcc cgccgcatgg tcgtcacggt
     3061 ggaccacccc ggctgggggc cgttcaccat gcccggcaac ccggtccagc tctccggctc
     3121 gccgacggag gtgcggccag cgccgctgct cgggcagcac aacgcggaga tctacaagga
     3181 gtggttgtcc ctgggcgcgg aggacctggc gcgactccgg gccgacggcg tcgtgtgatg
     3241 acatggggcc gacatggccc ccgaaccccc gaacactcgg ggcgcccccg acgcagtcgg
     3301 ggcgctcctc gacaacgcga ttcgatcgtc gactccgtga ccggcgagtg gctcaccact
     3361 cgaagccgta acgccggcgc atcaggtcga ggtacgccgc gtccggcatc tcgcgcccgg
     3421 tggcgacgta ctcctcgtcg gtcaggcgct cgcgaccggc cgcgagccgc cgggcgtctt
     3481 cgcccaccag gtaccggagc ctcggcgtgt cggtcgtcac cgcctcgtgg atcatgcggg
     3541 ccacgtcctc gggctggctc ggcatcttca tctgcatctg gtagaagagc agcaggcggc
     3601 gcacgtggac ggcatagggc gaggccgggt cggcgaaccg ccgggccttg ctgaagatcg
     3661 gggtgacgac gacgccgggc tcgatgatgg ccacgcggat gccgaaggcc accacctcct
     3721 gggcgagcgc ctcgctggcc gcctcgagcg cgtgcttcac cgccgagtag tggccgtggc
     3781 cggcgatggc cacccggccg gcgatcgagc tgacgttgac gatggcgccg ctccgctgct
     3841 cccgcatgcc cggcagcacg gcctggatca tccggatcgt gccgaagtag ttggtctcga
     3901 agagggtctt cgcccagtcc accggcacat cggcgatggg tccgccgccg ccgatgccgg
     3961 cgttgttgac gagcacatcg acgcggccgg agcggcggag cacctcgtcc actgcgcgcg
     4021 tcaccgaggc cgtgtcgtcc acgtcgaggg cgacaggtcg gatcgggagc gcgtcgacct
     4081 cgatggcctt cgagagatcg gtcgccgtgc cagggttgcg cacgccggcg tgcacgtcgt
     4141 gaccgagccg cgcgaagtgg agcacagtcg cgaggccgat gccgctgctg ctaccggtga
     4201 cgaggacgac ggacatgagg gtcctcccgt gagtcagcgt ccttggggcc ggtccattct
     4261 atctacatgt cggcccgtgg cgcgccaccc tggagctctt tcaccgaggg agtctggctc
     4321 gagcgcattc gtgctcgccg gacggcctac ccggtgctcg cgggacgtga ggggccggcg
     4381 gccgggcctg tcgctggtgg tttcgccggc gccgccgtag actcggcccc ccggggagca
     4441 gcgtggaccg agccatccgt gaccgagcgt gaagccctga cggccctgcg cggtgccgac
     4501 cccgcgcagg ccgcgcgcgc cgaggccgcg ctgtgggcga tgtggacccg gtccaccgac
     4561 tccgaggtca acgtgctcat gcaggacggg atcgccgcga tgcaggcgcg gcgcttcgcg
     4621 gacgccgagc ggctcttcac gcggatcacc gagtgcgccc ccgacttcgc cgaagggtgg
     4681 aacaagcggg ccaccgtgcg ctacctggcc gaggacttcg ccggcgccat caccgactgc
     4741 gaggcgacgc tggcccgcaa ccccaatcac ttcggggcgc tgtcgggcca gggcctctgc
     4801 cacctcgccc gtggcgaggc gcgcgaggcc gccgcgctct tccgccgcgc cctcgccgtg
     4861 caccctcatc tcggggccgc tcgcgagaac ctgcgcctcg cccttgccaa cgcggtgcag
     4921 ggcaatggac actgatcgca cgtcgcacac accgggagga gaccgcccgc catggccgga
     4981 gcgctgaccg tcgagacgcc gcacgacctg ctgtcgcacg tgggccggga gttcggcccg
     5041 tcggagtgga tgaccgtcga ccaggcgatg atcgacaagt tcgccgacgc gacgggcgac
     5101 caccagtgga tccacgtgga cgtcgagcgc gccaagcgcg agatgcccgg cggcaagacg
     5161 atcgcccacg gctacctcac gctctcgctg gtgccccgcc tggccgcgac cctggtgaag
     5221 gtcaacaagg tgacgcacgg cgtcaactac ggctcgaacc gtgtgcgctt caccgccacc
     5281 gtccccgcgg gctcgcgcgt acgtctgcgc ttgaagatcg ccaaggtcga gcccgtcgag
     5341 ggcaacgggg tgcgcgtgac gaacgaggtg acgatggagg tcgaggggca ggaccgtccg
     5401 gccctcgtcg ccgagatcat gggcatccgc tacgcctgac ggtggccccg acggccgagc
     5461 gtgacgattc atgaccgact cgaacgatcc ggcggagctg atcgaggcct acttcgatcg
     5521 cggctggacg gacggcctgc ccgtcgtgcc gccgaccgag aagtccgtcg cggcgatgct
     5581 ggccggcgcc gcgctcgaag gccgcgaggt gatcggcgag atctcgggcc gcaacacccg
     5641 cgtcaccgcc gacaaggtcg ccatcaacgc ggtactggcc ggatgccggc cggagtatct
     5701 gcccgtcgtc gtggccgccg tcgagggtct ctgccatccc gattttgcct accacggtcc
     5761 cgccagctcg accggcggct cggccatggt gctgatcgtc aacggaccgc tcggccgccg
     5821 gctcggcgtc aactccggca acaacgcctt cggccagggg gtgcgggcca acgccacgat
     5881 cggccgggcc gtgcgcctgg tgatgatgaa cgtgatgcag acgcggccgg gcctgctcga
     5941 ccgggccacg ctcgggaacc ccgggaagta cgccttctgc ttcgccgaga acgaggagga
     6001 ccatccgtgg gagccgctcc acgtcgagcg cggcttccgg cccgagcaga gcacggtcac
     6061 cgtctacgcc tcgaacagcc tctaccaggt gtacaaccag ctcgccgccg agcccgagcc
     6121 gctgctccgg tgcttcgccg acgcgctcgg caacctcggc attcccaaca tgaaggggtt
     6181 caaccaggcg ctcctcgtct tcgccggcga gcacagcgag gtcttccgcc agagcgggtg
     6241 gacgaagcgc cgggtgaagg agttcctcgt cgcgcacacg cgacgcccgg tgtccgactg
     6301 gaagcgggcc gcccggctgc ccggcgccgt gacgccagat gacgagacca ccctgcgcgc
     6361 gctgtttgag gatcccgacg atcttctgct cgtctgcgcc ggcggccggg ccgggagctg
     6421 gtccgctggc ctgccgggct ggggcaagaa gtggacccgg agcgtcacca cgctgatccg
     6481 tgaaatatca tgacgtcgtt cgggccgacg ccgtcgatcc gggggtggcc atgaggaagc
     6541 tgctcgatcc cacgtcgtca ccggtggcgc gcgacgtcgc ggtggctgca cgcccggcgt
     6601 cgctccgcgg gctcaggctc gcgctcgtcg agaacaccaa gttcaactcc gacgcgctcc
     6661 tcaccaggct cgccgagcgg ctggcccgcg accacggact ggccacgacc gtcatgcatc
     6721 gcaagcgctc gcccagccac gaggtcgagg cggcggcggt ggcggagctg cgcgcgaagg
     6781 ccgacgtcgt catctccggc atcggcgact gaggctcgtg ctcgtcgagc agtgtgctcg
     6841 acgcgatcct gctggagcgg gccggcgtgc cggcggtggc ggtggtgacc gagccgttcc
     6901 aggccaccgg cgaggccatg gccgcgagct ggggaatggc cggctatcgc ttcctcacca
     6961 cgccgcaccc catcgccaac ctcggggaca aggacctgga cgagcgggcc gagcgcctgg
     7021 tcgcgccggt cgtggcgttg ctgcagaacg cctgaagcgc atgcggcgat ggcttggcga
     7081 cttccgagca tgtccacgaa catccgccgt gcagcgttcg gccccgaacg ctgtaccgct
     7141 ggctcgcaag cgctcgccgc ggccgctcac cgcgatgttc gacggcgcat gaccctggcc
     7201 ggaggagatg atgcgaaggt ggagggccct ccatgatcca cacacctgtc tgtgacctgc
     7261 tgcagatccg gcacccgatc gtgctgggtg gtatggccgg ctccacctcc gtgcccctcg
     7321 tggcggccgt cagcaacgcc ggtgggctgg ggacgctggg catcgcgcgc accccggccg
     7381 agcagattcc aagggacatc ggcgccatcc gggaagcgac caaggggccc ttcggcgtca
     7441 acttcctgct cttcgtggct cgtgagcccg ccctccaggc ggcgctcgac gcgcgtccgc
     7501 ccgtgttctc cgccgcctgg gcgcggccgg atcaggacct ccgggcgctc ttcggccgtg
     7561 cgcacgaggc aggtagtcag gtgatgtaca tggtcggcac ggtgccggag gcggtgcgcg
     7621 cgaaggcagc gggcgcggat gtgatcgtgg cgcagggcac agaaggaggg gggcacgtcg
     7681 gctggatggc cagcatggcc gtgctgccca tgatcgtgga cgcggtcggc ccgacgcccg
     7741 tggtggcggc cgggggtatc gcggacggcc gcgggctggc cgccgcgctg gcactcggcg
     7801 cgcagggcgc gctgctgggc acacgcttcc tggccacgaa ggaatcgccg ctgcacctca
     7861 acttcaaaca ggcggtgctc gacagcgacg ggcacgacac ggtgttgacg gagattcccg
     7921 acatcgcgcg cgccaacgtc tggccgggcg ccatggcgcg cgcccggcgc aatgccttca
     7981 tcgagcgctg ggcgggtcgg gagtgggagt tgcgcggacg gcagccagag gccgccgcgg
     8041 ccttggaaag ggctcgcgag accggcgacg tggacaacgc ctctctgctc atcgggcagg
     8101 acgcggggtt gatccacgac atcccgcccg cgggcgagat cgtggaacgc atcgtggccg
     8161 aagcggaagc gctgctgaag gaccgcctgc ccaagctggt gcgcgtgggc tgaggcctcg
     8221 ggcttgtgcc gctcgacgac tgcctacgtg acagccagga ccgcgtcgcc tgctcgccgc
     8281 tgcacgggcc gccgacctgc gacgttcgac gcgccggtcg gaggtgcagg cgtggccagc
     8341 gtgcgatcgg gtcagtcgat ggatgcgtgc acgcagttgg agatcacgtc caggcgcgcg
     8401 ctgtgccagg ggtcgccgac gcggctgttg gtcaggtacg cgaagctgac gccggagtcc
     8461 ggatcggccc agcagtaaga cgagcccacg ccgccgtggc cgaacgtgcg cggcgaggcc
     8521 aggctgccga ggccgcggat ggtgtcggtg gtgccgcgca ggtgcggtcc gagcccgcgg
     8581 tgcatcggca tgcccatgta gccgtccacc atgtcgccgg tgacgttgcg cgtgacgtac
     8641 tgcaccgttc gcggcgacag cagccgcacg ccgccgagcc ggccgccggc cagcatcatc
     8701 tggtagaagg cggccattgc ccgggcggtc gcgtagccgc cgctggccgg cgctccggca
     8761 cggcggaagg gcgcggtgtt ctcgtcggcg cgcttcacct ggcggccgcc gtcggccgac
     8821 ggctcgtgca tgtccacggc gcgcccgtgc tcggtgtcgg gcaggccgac gaacagctcg
     8881 tcgcccaggc cgaggggcac cagcacgttg tcgcggatga acgcgcggta gtcggtcttg
     8941 gtgatcgcct cgatcatcgt cgcggccgtc cagtgagcgg atcgggcgtg atagtgcagg
     9001 cgcgcgcccg gcgtccactc gagggtgaag ccgcacacgg tgcgccggag cagctcgtgg
     9061 tcctcccacg cttccttggg tacgtcggcg ttgggaaagc cggcccggtg cgagaggagc
     9121 tggacgatcg tgatgtcgcc cttgccgttg gcctcgaagc ccggcaggtg ctcggccacg
     9181 cggtcggtga agcgcagggc gccgcgctcg gcgaggagcc acaccgcgca ggcggtaatc
     9241 accttggtgt tcgagaagag cagccacagg gtgtcgtcgc gggcggccac ccgctgggga
     9301 tcgaggcgcg cgtcaccgta ggtcttcacg agcgcgagct tgccctcccg ggcgatggcg
     9361 acctgagccc cggggtagcg gccctcggcg atgtggcgat tgatcagctc gtcgagccgg
     9421 ccgagcgccg tcgcgtcgag gttgagggcc gcgggagtcg aggtctcgag aggaaagcgc
     9481 tgggccatgt cgtgcctccg ggggaatgtc ggctcggggc aggatacctc aggtgatgac
     9541 cttgcgcctc gggctgcggc gggcggcgag ggcgagcact tcgtcgagcc gctcgggctc
     9601 gacgggcttg gtcagatgca ggtcgaaccc cgccgcgagc gctttcctcc gatcggtcag
     9661 ctgcccgtaa cccgtcaccg ccacgagcac cacggcgttg ccctcggcgg tgtcgcggat
     9721 ccgtgaggcc acctggaggc cgtcgagtcc gggcaggccg atgtcgatca gcgcgagctg
     9781 cgggttcacc cgacaggcca cccgcaccgc ctcgaagcca tcggccgcct cgtggacctc
     9841 gtgaccggcg agttcgagca ggctccgcag cgtctcccgg gtgtcgcggc tgtcctcgac
     9901 gaggaggatg cgaaggggtg cccgatcgcc ctggcctggc ccgcgcccgc cgtcatcgat
     9961 cttctcgcgc gtcgcgcggg gcagccgcac ggtgaacacg ctgccgcggc ccggcccctc
    10021 gctcgccgcc gccaccgacc cgccgtgaag ctcgacgagg cgccgcacga gcgtcaggcc
    10081 gatccccagg ccgccctgcg cgcggtcgag ggcctgccgc ccttgcgtga acaggtcgaa
    10141 gagccgcggg agcatctccg cgctgatccc gatgcccgtg tccctgacgc gcacgacggc
    10201 ctcgtcgccg tcggcgcgga cctcgacgtg gatgcatccg ccccgatcgg tgtacttcgc
    10261 ggcgttgacc agcaggttgg tcaccacctg ttcgagccga tcgaagtccg catcgacggt
    10321 gatcgagtcg gccttcacgg tgaccgcgtg atgctcgagc cgcccggaca cgcgaagggc
    10381 gtcgagggcc cgggccacga cgtcggcgag gtcgatgggc gcgcggtcga gggcgatctg
    10441 gccgacggtg agccgggcga cgtcgaggag gtcgtccacc atccgcgcga ggtgaccggc
    10501 ctgacggccg atgacctcgc gggcccgcgt cgcccgttcg tcggcggtgc cgatgacctc
    10561 cagcagccgc accgcggtgg tgatggcgcc cagcgggttg cgcaactcgt gcgagagcat
    10621 ggcgaggaac tcgtccttct ggcggtcgcg ctcgtggatc tcctgataga gccggctgtt
    10681 gtcgagcgcg atcgcggcac ggccggcgat gtcccggacg aggtcgaggt ccgcgtcgga
    10741 gtagcggcgc ggcagccgca tcagggatgc catggcgccg aacgtgcgcc cgcgcgcgac
    10801 cagcggcagg atgatgccct gggtgtgctg cccgtcgtgc gacacgatcc gctcggcccg
    10861 gtcggcggcg acgacgccct cgagacgggc gttcagggcg aggccgtcgt cgtccggcac
    10921 cgggtcccgg ctcacgcgcg cggccggccg ggcgtcgagc cagctcgtct ccgccgtgtg
    10981 cccgccggcg tcggtcagcg agatggccgc cagatcggcc accagcggca cgaagagcgc
    11041 gagcagatcg gccacgagcg tcctggtgtc gagcgaccgg gtcaggacgc cgctggcctc
    11101 ggcgagcacg cgcaggcgcc ggctctcctc ctcggcggcc gcgcgggccg cctgcgcgcg
    11161 cgagagggcg atgcggtgct ccgcctcccg ccgcatccgc tcgtgtatcc tggacagctc
    11221 gacaaagacg cgtaccttcg cgcgcagcac gtcgggcgcg aacgggttga agatgtagtc
    11281 gacggccccc agcgagtacg cgcgcgccgc ggccatctcg tccatgtgcg ccgtcaggaa
    11341 gatgatgggc gtgtggcgcg agcgaggacg ctgacgaacg agcgaggcgg tctcgaagcc
    11401 gtccatgtcc ggcatggtga cgtcgagcag gatgacggcg acgtcgtggt ccaggagcac
    11461 gcgcagggct tcgacgcccg agccgacggc gatgatctcc tcgccgagct cggccagggc
    11521 ggcctcgatg gcctgccgct ggccgggcat gtcgtcgacg acgaggacct tgacgcgact
    11581 gccaggcgag gcctccaccg ggagcgcctc tagggggagc cttcgaagcg gcacgggccc
    11641 acgcagacgc gtctcaccgg ttgtgcgctg ggcgaccgag acacggtggg gcagcgtaac
    11701 gaggggctgc ggagcgccac aagccggaaa acgttttccc actcgcgtaa ttcctacaac
    11761 tgccggccac ggttgacgag cggggcggca tgcagtatct aggcgccagc gcatggccat
    11821 ggccgacacg acgatccgcg tgggcttcgt gggcgccggc aacaacaccc ggcgccggca
    11881 catcccgggg ttccagaagc tggccggggt ggagctggtg gccgtggcca accggaccaa
    11941 ggagtcgagc gagcgcgtcg cgcgcgagtt cggcatgcgg ggcgtgtatg cggactggcg
    12001 cgacctcgtc cgggctcccg aggtggacgc tgtctgcatc ggcacctggc cgaacatgca
    12061 ccgcgagatc acggaagccg cgctcgcggc gggcaagcac gtcctctgcg aggcgcgcat
    12121 ggcgatgaat gccgcggagg gccggcgcat gctggaggcc tcccgcgggg cgccgagcct
    12181 ggtcacgcag ctcgtgccgt cgccgccgac gctcgaggtc gactcgacga tccaggcgct
    12241 cctggccgac ggctacctgg gacgggtgca ggccgtcgag ctgcaggcca cgcagaacgc
    12301 gggcttcgtc gacgccgacg cgccgctgca ctggcgccag gacatcgcgc tcagcggcca
    12361 caacgtcctg aacatgggca tctggtacga ggcggtgctg cgctggctcg gcccggcccg
    12421 ccgcgtgatg gcgatgggga gggtcgcggt gccgcggcgt cgcgatgccg gcggtgcctg
    12481 gcacgacgtc cgcgtgcccg atcacatcga catcctggcc acgttcggcg agggcaccgt
    12541 cggccgactg cgcttcagct cgctcacggc cctggcgccg ccgagcgagg tgtggatctt
    12601 cggcagcgac ggcaccctgc ggctcgaggc cgacgcccgc cggctctcgg gcggtcggcg
    12661 gggcgaccgc gagctgcgcg agatcgcgat tcccgccgag cggcgcgtgg gctggcgggt
    12721 cgaggaggag ttcgtcaacg ccatccgcgg ccgcgagaag atcgcgcgca cgacgttcga
    12781 ggacggcgtt cgctacatgg agttcaccga cgcggtcgcg cggagcgtcg cgtcgggcca
    12841 ggccgtcgac gtcgccgagc tctgagatct cgcccgtggg ccggctcgcc gccctcccgc
    12901 cgctgccgac gaccgtggtc ggcagctatc cccagcccga gtggctgatc gaccgcgacg
    12961 tgctgctgcg caccggagtg ccgcgggtgg gactgccggg gctctggcgg gtgccggcga
    13021 cgctgcggga gcaggccgag gacgatgccg tgcggctggc cgtgcgcgac atggagcggg
    13081 ccggtatcga cgtcgtcagc gacggcgagg cgcgtcgcga gagctacttc aactggctcg
    13141 cgccgttcct caccggcatc gacctcgagc ggccggggac cgcgatcagc cgcaccggca
    13201 agcccacgcc ggtgccacgc gtggtggggc cgatcacgcg cccccagccg gtgctcgcgc
    13261 ggcacaccgc cttcttgaga gcggagaccg atcgtccgat caaggtcacc gtgcccggac
    13321 cgttcacgct gaccgcgctg gcccagaacg agcactaccc cgacgccccg agcctggcgc
    13381 tggccttcgc cgacgcggtg aacgcggagc tacgcgatct gagatccgcc ggcgccgatt
    13441 tcattcagct cgacgagccc tacctgcagg cgtggccgga ccgcgcgcgc gagtacggcg
    13501 tggccgtgat cgatcgcgcg ctggcgggcg tggccgggcc gacggtgatc cacctctgct
    13561 tcggctacgc gcacaccgtg ggcagcgcga agcccagcgg ctactcgttc ctgcccgagc
    13621 tggaccgatg ccgcgggacc cacatctcca tcgaagccgc ccagccgcgc ctggattgcg
    13681 cggtgctccg gtcgctgccg tccaagacga tcgtcctggg cgtcctcgac ctgaacgatc
    13741 ccgccgtcga gacgccggag atcgtcgccg accgcatcag gaaggcgctg gctcacgtgc
    13801 cgtccgagcg cctcgtcgtg gcgcccgact gcggcatgaa atacctgccg cgcgagcgcg
    13861 ccggcgcgaa gctacacgcg ctcgcggccg gcgcccgcct cgtgcgtgcc gagctcgccg
    13921 gctagttttc gcgaatcagg agttcgctgg tgaacgcgcc ggccggggcg gggtcggagg
    13981 ctcgacggag ccgccgcgcg aggtgacgac gggacaccag cggctagcgc cagtcgtcca
    14041 cggacaccgt cttctggtac gtgcagccca tgatgcgcca gtagccgttg gtctcgccgc
    14101 cgaccaccac cgcgtgcacg tcctcgcgcc ggaacatcgg aatcagctcg tcgggcgcgg
    14161 cccggagccg cgaggcccac ggctcttcgc cgaaggtggc gcgaggataa atgtagttct
    14221 gcacgagctg gtagtcccag tactcgcgcg cgcgcaggcg cgcgatctcg tggatccagt
    14281 cgagcatggc cttcttctcg acgaacccgc cgcgctcgac gaactggcgc gcggcgatgg
    14341 ggtcgagcac cagcgtgggc ggcccggtgg ggtccatgcc gcgcaacagg ttggccacgt
    14401 gctcgcgcca gtgcgtctcc cgcaggccga gcgtgaaggc ggtggaccgg cagcccgtga
    14461 acacgctcac cgcgctgtcg ccgggcgcga agccgtggtc gcagtggaag ggctcccacg
    14521 ggctccgctc ctcgttctcg gcgaaggtga cgccggcgta cgcgtaggcg ttgccctggt
    14581 tgcccatgta ggacaggccc gggaccgagc cgccctggag gttctgggag aggagcgagt
    14641 aggcgcggcc gatggtggcg ttggcctggc tgtacggccc catcgcgccc gtgcccgagt
    14701 tcatgccggt ctcgtggcgg atcgggccgt tcaccacggc catggtggcc atggcgctcg
    14761 aggagctgct gcgcgcggtg acgcccgtgg cagcgagcgc gaggatgacg gggaggtact
    14821 cgggccgggc gccggccatc accgcgttca ccgcgacctt ctccaccgag tacgcccagg
    14881 cctcgcgcgt gtgagtcgag cgcatgcgtc cgaccggctc gtcgggcttg cggcgcgtcc
    14941 ctgcgagcat cgccgccacc cgcgcctcgg tcggcagcac gatcggcagg ccgtcggtcc
    15001 agtggcgctc gaggaagagc tggcgcaggg actcctcggt ggccggcggc agcaggcgcg
    15061 gcgtcgagcg gtcgaacccg tcgggcccgc gctcgtcgtc ggtgatcggc cgcgtgaggc
    15121 cctccaggat ctcctgcatc accggccgcc cgctgatcgg atcggtgccg tccacgtacg
    15181 cccgcagctc gcgcggtgtc ttgcccatga ccggctgggg cacgaaggcc tgccgcaggc
    15241 cgggcatgcc ggcgacctgg gccaccgagc gcacgacgcg gtcgaacttg tcggtgtgga
    15301 gcgcgaccgt ggggacgccg tacttcgtct ccagcgtcat cgcgtgcccg gcgaccgccg
    15361 gggcacacgt gcttcagtgg ccgacgccga gcaccgcggc gtggccgcgc tgccggatct
    15421 cctcccaggt cgcggggtcg tccaccagat agacgctcga gatcggcttg aggacggtgc
    15481 gcacgccggg catgtgctcg ccgagccacg cctgtacctg ctcgagcagc aggcccgagt
    15541 cgtcgaaccg gcaatcgacg aggtagaccg tgcgtccctc gagcgtcggc agacgcgggg
    15601 ccaggtcctt gcgactgacg accggcggga gcccggtcgg gtcgaacacc gtcatccgtt
    15661 cgctcatgtc tgcgcgctcc tggttcgctg gaccgtccgg ccgcgacggc tccacgccgg
    15721 cgtcgcggcc gattccgcca tgagcgtgat cgtagtcgag ccgcgcgggt tacaccagag
    15781 ccggtgaggc gttctctccg cgggcgtcgc cgccccggcc cctcgggcgt ctcgaacccg
    15841 tgggattgac accgcccgga cgacggtcgt tcaatccatg tgaggaggcg agcgagatgg
    15901 cggtggcgct ccaaccctac cggttcacgg tcgaccagta ccacacgcgc tccacctcgt
    15961 cgaggtcgcc gacacgtcgc tcgagcgcga ccgcggtcgc aagctgccgc tctacgcgcg
    16021 taccggcgtg ctcgaggtct ggctcgtgaa ccggcagacc gacgcgatcg agatgttccg
    16081 cgcgtgcgaa gtggcgcggc tcaccgtagc tctcgcccga gggaccgtcg ctcgctccag
    16141 ccgcttttcc cgatctcacg ctccgtgtcg acgacatcct cgggagcacg cgggcctgag
    16201 tacctccgga gcatggcccg gttccccgtg atcaccgacg cggcgctggc ggagctgcgc
    16261 cagcgcatcg gccggccggt gcgccgcccc gagccgtaca tcgaggttgc cacgcgcgat
    16321 gcggtgcgcc actgggcgca cggcatcg
//
LOCUS       DATIAZ010000206         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1240330, whole genome shotgun sequence.
ACCESSION   DATIAZ010000206 DATIAZ010000000
VERSION     DATIAZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240330"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VLF19_07420"
     CDS             <1..668
                     /locus_tag="VLF19_07420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSE93119.1"
                     /translation="KVGALGTYRALGLAKARGARFLLASTSEVYGDPLVHPQREDYWG
                     NVNPVGPRGVYDEAKRFAEAITMAYHRTHGIDTRIVRIFNSFGPRMRLNDGRAIPAFA
                     TQALTGQPLTVFGDGSQTRSFQYIDDLIDGVWRLMDASVNDPVNIGNPQEMTLLELAK
                     RIIRLARAQSEIVFRPLPVDDPKVRQPDITRARHLLGWEPRVDTDEGLRLTIDWFRTR
                     LAS"
     gene            665..1594
                     /locus_tag="VLF19_07425"
     CDS             665..1594
                     /locus_tag="VLF19_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSE93120.1"
                     /translation="MRTLVSGGAGFIGSHVVDRLLADGHAVDIVDNLSTGRRERVDGR
                     ARLHVCDLRSARLDAVFAEARPEVIVHVAAQAAVARSVIDPLLDASVNVLGTVALLEA
                     GRRAGVRRAIYTSTGGAAYGDTDVVPTPEDHPTRATSPYGVSKVAAERYLECWAGLTG
                     ASALTLRLANVYGPRQDPQGEAGVVAIFSHRLLRGEACLVNGDGEQTRDYVYVEDVAE
                     AVSRAVARPSVTGVANIGTGLETTVNALYARLARLAGVTRPAAHGPAKPGEQRRSVLD
                     ASRARALLDWTPATSLDDGLARTLTCFKAEPGA"
     gene            1610..1777
                     /locus_tag="VLF19_07430"
     CDS             1610..1777
                     /locus_tag="VLF19_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSE93121.1"
                     /translation="MDDELKKILVCPVCKGALRYEDTRIICPACRKAYPIRDGIPVML
                     IDEAQSWSPTG"
     gene            1837..>2854
                     /locus_tag="VLF19_07435"
     CDS             1837..>2854
                     /locus_tag="VLF19_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HSE93122.1"
                     /translation="MRPLTLTRAKPVVPLCNRPFLAWQLALLREHGVDDVVLSCSYRV
                     DDLRSALGEETFGVKLRYVIEVEPLGTGGGVRNAADIARGHVFVLNGDILTDADLGAM
                     RRFHEARDARVTIFLTPVPDPRQYGLVETSADGRIRSFREKPAAGEAITTDTINAGIY
                     LIDAELLHRMPVDRAVSIEREFFPALIADGVPCYGWAPAVYWRDIGNIATYREAQMDL
                     LDGRVRVSLSPPGEGRGGVWLAPGATLAPDAHVRPPVAIGEGARVGPGAAVGPRAVLG
                     PGCVVAAGATVEEAVLWERVEVGPRAVLRGCVVVSDARIGAGAEIGAGVVVESGAVVA
                     DHARP"
BASE COUNT          383 a         1041 c         1041 g          389 t
ORIGIN      
        1 tgaaggtcgg cgcgctcggc acctaccggg cgctcggcct cgcgaaggcc cggggcgctc
       61 gcttcctgct ggcctcgacc tccgaggtct acggcgatcc gctggtgcat cctcagcgcg
      121 aggactactg gggcaacgtc aacccggtgg ggccgcgggg cgtctacgac gaggccaagc
      181 gcttcgccga ggccatcacc atggcctatc accgcacgca cggcatcgac acgcgcatcg
      241 tccgcatctt caactcgttc ggtccccgaa tgaggctcaa cgacggccgc gcgatacccg
      301 ccttcgccac gcaggcgctg acgggccagc cgctcacggt gttcggcgac gggtcgcaga
      361 cgcgctcctt ccagtacatc gacgatctca tcgacggggt ctggcggctc atggacgcgt
      421 cggtgaacga tcccgtgaac atcggcaatc cccaggagat gaccctgctg gagctcgcca
      481 agcggatcat ccgcctggcc cgcgcgcaga gcgagatcgt gttccggccc ctgcccgtgg
      541 acgaccccaa ggtgcgccag cccgacatca cccgagcccg gcacctcctc ggctgggagc
      601 cacgcgtgga cacggacgag ggcctgcgcc tcaccatcga ctggttccgc acgcggctcg
      661 cgtcgtgaga accctcgtca gcgggggtgc gggcttcatc gggtcgcacg tggtcgaccg
      721 gctcctcgcc gacggccacg ccgtggacat cgtcgacaac ctgagcaccg gccgccggga
      781 gcgcgtcgac gggcgcgcgc ggctgcacgt gtgcgatctg cgaagcgcgc ggctcgacgc
      841 ggtgttcgcg gaggcccggc cggaggtcat cgtccacgtc gccgcccagg cggccgtcgc
      901 gcgctcggtg atcgacccgt tgctcgatgc gagcgtcaac gtgctgggca ccgtggcgct
      961 gctggaggcg ggccggcggg ccggcgtccg gcgggccatc tacacgtcca ccgggggcgc
     1021 cgcctacggc gacaccgacg tggtgccgac gccggaggat catcccaccc gcgccacctc
     1081 cccgtacggg gtctcgaagg tcgccgccga gcgttacctc gagtgctggg ccggcctcac
     1141 cggcgccagc gcgctgacgc tgcggctggc gaacgtgtac ggcccccggc aggacccgca
     1201 gggcgaggcc ggtgtcgtcg ccatcttcag ccaccggctg ctccgcggcg aggcgtgcct
     1261 cgtgaacggc gacggcgagc agacgcgaga ctacgtgtac gtcgaggacg tcgccgaggc
     1321 cgtcagccgc gcggtggcgc gtccatcggt caccggcgtg gcgaacatcg gcaccggcct
     1381 cgagaccacc gtgaacgccc tgtacgcgcg gctggcgcgg ctcgccgggg tgacacggcc
     1441 cgccgcgcac ggcccggcga agcccgggga gcagcggcgc agcgtgctgg acgcgtcgcg
     1501 cgcgagggcg ctgctcgact ggacgccggc gacgtcgctg gacgacgggc tcgcgcgcac
     1561 gctcacctgc ttcaaggccg agccgggggc ctgaggagag gctcgcatga tcgacgacga
     1621 gttgaagaag attctcgtgt gccccgtgtg caaaggcgcc ttgcgttacg aggacacgcg
     1681 catcatctgt ccggcctgtc gcaaggccta tccgatccgc gacgggattc ccgtcatgct
     1741 gatcgacgag gcgcagtcct ggtcgccgac cggctaggcg cgggcgtcgc ccgcgccacg
     1801 caggcgatca tcctcgccgg gggccagggc acgcgcctgc gccccctcac gctcacccgg
     1861 gccaagcccg tggtgccgct ctgcaaccgg ccgttcctcg cctggcagct cgccctgctg
     1921 cgcgagcacg gcgtcgacga cgtcgtgctc tcttgctcgt accgcgtgga cgacctgcga
     1981 tcggccctgg gcgaggagac gttcggcgtg aagctgcgct acgtgatcga ggtcgagccg
     2041 ctcggcaccg gcggcggcgt gcgcaacgcc gccgacatcg cgcgcggtca cgtgttcgtg
     2101 ctcaacggcg acatcctcac cgacgccgac ctcggggcca tgcgccggtt ccacgaggcg
     2161 cgagacgcgc gcgtgacgat cttcctcacg cccgttccgg acccgcgcca gtacgggctc
     2221 gtggagacga gtgctgacgg ccggatccgg agcttccggg agaagccggc cgccggcgag
     2281 gcaatcacga ccgacacgat caacgccggc atctacctca tcgacgccga gctgctccac
     2341 cggatgccgg tggaccgggc ggtctcgatc gagcgcgagt tcttccccgc gctcatcgcc
     2401 gacggagtgc cctgctacgg gtgggcgccg gcggtgtact ggcgcgacat cggcaacatc
     2461 gccacgtatc gcgaggcgca gatggacctg ctggacggcc gggtgcgcgt ctcgctcagc
     2521 ccgcccggcg aggggcgggg cggcgtgtgg ctggcgcccg gcgccacgct cgcgcccgac
     2581 gcccacgtcc ggccgccggt ggcgatcggc gagggcgcgc gcgtgggacc aggcgcggcc
     2641 gtcgggccgc gggcggtcct cggccccggc tgcgtcgtcg ccgcgggcgc cacggtcgag
     2701 gaggccgtgc tgtgggagcg cgtcgaggtg ggaccacgcg cggtcctgcg cggatgcgtc
     2761 gtggtctccg atgcccggat cggggccggc gcggagatcg gcgccggtgt ggtggtggag
     2821 tcgggcgccg tcgtcgccga ccacgcgcga ccga
//
LOCUS       DATIAZ010000207         6653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_846627, whole genome shotgun sequence.
ACCESSION   DATIAZ010000207 DATIAZ010000000
VERSION     DATIAZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846627"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /gene="hemG"
                     /locus_tag="VLF19_07440"
     CDS             <1..1272
                     /gene="hemG"
                     /locus_tag="VLF19_07440"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HSE93123.1"
                     /translation="EAGPDSFLSEKPWALALCRRLGVEHRLARTDDRHRRTFVWFRGR
                     LHPLPDGFQLLAPTRLVPFASSGLFSWAGKARMALDLILPRGGARMPAGAAGADESLG
                     SFVRRRLGREALERVAQPLVAGIYTADPEDLSLAATMPRFLELERRERSLILGLWRAS
                     RRAPDSAAGTSGARWSLFVTFAGGMGEMVDTLAAHLPAGAVQLERHVSGIERHGDRWR
                     VEGGDGARVEADRVVVATEAHAASRLVRYVDPSLATLLRVIPYAGSATVSLGYRRADV
                     PHPLDGFGFVVPRSELRTLLACTFSSVKYPGRAPEGHVLLRGFVGGALDAAALEGDDA
                     VIVGRVQDELRAALGITAEPVLVRVGRHPASMPQYLVGHGRTVEAIERHLDGTPGLLL
                     AGSAYRGVGIADCVRSGEAAAERALLGATAE"
     gene            complement(1538..2500)
                     /locus_tag="VLF19_07445"
     CDS             complement(1538..2500)
                     /locus_tag="VLF19_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HSE93124.1"
                     /translation="MGKIEPEKIRLGVSACLLGERVRYDGGHKRDAFLADTLGPFVEW
                     VPVCPEVEIGLGVPRPTLRLVGAEAVPRLVVEKTGEDLSRRMRSWAEARVTELERLGL
                     DGYVLKRGSPSCGLFRVRVYSEDGQPGRVGRGLYADALARRLPSLPVEEEGRLTDPVM
                     REHFIGRVFATARWRDFRAGAPRPRDLVAFHTAHKYAVLAHSPARYSRLGRLVAAAGP
                     ALPRATLDEYGALLLEALAVRATRGRHANVLAHLAGYVKRDVTAEERAELAETIADYR
                     RGLVPRVVPITLIRHHARRLGLAHLAAQVYLNPHPKELMLLNHA"
     gene            2523..3020
                     /locus_tag="VLF19_07450"
     CDS             2523..3020
                     /locus_tag="VLF19_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSE93125.1"
                     /translation="MLEPLSRTPSLSPTAQRVQDVLTAGGYPYQVVESSVSTRTAAEA
                     AKLVGCEAGQIAKSLVFKTEKTGRAVLVIASGVNQVNEFRIGMYLKEAVVKAPASLVR
                     QATGFAIGGVPPLGHATAMETFIDQDLMKHAEIWAAGGTPNALFKLVPADLTKMSGGR
                     VVKVT"
     gene            3055..3279
                     /locus_tag="VLF19_07455"
     CDS             3055..3279
                     /locus_tag="VLF19_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93126.1"
                     /translation="MLEAVTMKEIDAIFAVTDALGIHRERLTIPLGPASPGRVRRLPS
                     GKLEIVVEARRPLDEWLKELPALIAAAGRT"
     gene            3746..4723
                     /gene="corA"
                     /locus_tag="VLF19_07460"
     CDS             3746..4723
                     /gene="corA"
                     /locus_tag="VLF19_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HSE93127.1"
                     /translation="MSGVVNCAAYAGGRKVADVAVDDISEVLKRPDVFVWIGLHEPDE
                     PLLRRVQAEFGLHDLAIEDALRAHQRPKLERYGDSLFVVLRTAVIDGGDRRIAFGETH
                     IFVGERYVVALRHGATHSYTDVRARCEAAPMQLAKGPGFVLYALMDFIVDQYFPVADL
                     LEDELETLEENVFGEAFSRETTARIYRLKRDLLDVKRAVSPLVDVCNRLMRFDLELIP
                     EDTRLYFRDVYDHVIRINEMVDMLRELLTTALEANLSLISVSQNDAMKRLAGWAAIIA
                     VPTMVAGIYGMNFRFMPELSWRWGYPTAMAVMVGTCGFLYYKFKRSGWL"
     gene            complement(4839..5189)
                     /locus_tag="VLF19_07465"
     CDS             complement(4839..5189)
                     /locus_tag="VLF19_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93128.1"
                     /translation="MTLRRRLVITVCPTEPGSVRLPVRPGERARRLDARAIADLLERM
                     AGERGLEHHVRVRRACAGGCAGQGPNVSVAIHPVPRPGEPEDHVAIGWRSYVASIARL
                     DCLATIIDENLAPR"
     gene            complement(5186..6064)
                     /locus_tag="VLF19_07470"
     CDS             complement(5186..6064)
                     /locus_tag="VLF19_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HSE93129.1"
                     /translation="MPANRHDMAVAVGVDLGGTWMRLHATAGPRRLATWRRTAPPPAR
                     MIDALRAVWNQRGWRRRDVAALVIASRGVWTAGERRALARRLAPLARRVLALSDAEAA
                     ALGALSGRPGVLLLAGTGSIAIGHDALGRWRRAGGLGPLLGDEGSAFWIGHEWIRATV
                     DDGAPPARRIARTPDAIARIAARAPAVLRAARAGDRRARAIVREAQRHLARLAASVVR
                     ELALPAPVTVTWAGGLLADAWFRAGVRRALTRTGPPARWRTPRAPAVVAAARLAAQLA
                     AGARPPAAPPGTARRA"
     gene            6193..>6653
                     /locus_tag="VLF19_07475"
     CDS             6193..>6653
                     /locus_tag="VLF19_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93130.1"
                     /translation="MGKALATWLLVSAAAASSAVTPRDVVQSAVVRVVQAIEESHLAR
                     PEPPAKPVGGKLRLEIRRVAAELFDFEEISRRALSRHWAGRSSAEQAEFVALFTDLLE
                     RAYFGRIEAYSGEKIVYTGEAVDGDYATVRSRILTRRRTETALDYRMHKIAG"
BASE COUNT          852 a         2423 c         2459 g          919 t
ORIGIN      
        1 gaggccggcc ccgactcctt cctctccgag aagccctggg cgctggcgct ctgccgccgc
       61 ctcggcgtcg agcatcgcct cgcgcgcacc gacgaccgcc atcgccggac gttcgtctgg
      121 ttccgcgggc ggctgcatcc cttgcccgac ggcttccagt tgctggcgcc gacccgcctc
      181 gtgcccttcg cttcgtcggg gctcttctcc tgggcgggca aggcgcgcat ggcgctggac
      241 ttgatcttgc cgcgcggcgg cgcgcggatg cccgccggcg cagcgggcgc cgacgagagt
      301 ctcgggtcgt tcgtccggcg gcggctcggg cgcgaggcgc tcgagcgcgt cgcccagccg
      361 ctcgtggccg gcatctacac ggccgacccc gaagacctga gcctcgccgc caccatgccg
      421 cgcttcctcg agctggagcg ccgcgagcgg agcctgattc tcggcctctg gcgggcgagc
      481 cgccgcgcgc cggattcggc agcggggacc agcggggcgc gctggagtct cttcgtgacg
      541 ttcgccggcg gcatgggcga gatggtcgac acgctcgcgg cgcacctgcc tgccggcgcc
      601 gtgcagctcg agcgccacgt cagtggcatc gagcgccacg gcgaccgctg gcgcgtggag
      661 ggtggtgacg gcgcgcgtgt cgaggctgac cgcgtggtgg tcgccaccga ggcgcacgcg
      721 gccagccggc tggtgcgcta cgtcgatccg tccctggcga cgctcctccg ggtaattccg
      781 tacgcaggct cggccaccgt gtcgctcggc taccgccgcg cggacgtgcc gcacccgctc
      841 gacggcttcg gcttcgtggt cccccgctcg gagctccgca cactcctggc ctgcaccttc
      901 tcgagcgtga agtatccggg ccgcgcgccc gagggccacg tgctcctgcg cggcttcgtg
      961 ggcggcgcgc tcgacgccgc ggccctcgag ggcgacgacg ccgtcatcgt cggccgcgtg
     1021 caggacgaac tgcgcgcggc cctgggcatc acggccgagc ccgtgctcgt gcgcgtgggg
     1081 cgccatccgg cgtcgatgcc gcagtacctg gtcggccacg gccggaccgt ggaggcgatc
     1141 gagcgccacc tcgacggcac gccaggcctg ctcctggccg gcagcgccta ccgcggcgtc
     1201 ggcatcgccg actgcgtgcg ctcgggcgag gctgccgccg aacgcgcgct gctgggagcg
     1261 acggcggagt gatcgcggca tcggggtttc ccgagcggga tctggcagtg tgtcgcccgg
     1321 gtcggcgccg acgttttgcc cgcggtccag gtcttcaatc catggtgcag gccggcgcct
     1381 cgatcccagg gcgcggtacc tccagctcgg tgcttggagc tcgccagcgt agcgggtctc
     1441 gaacgtccac acaccgtagg agatcggagg cgccgccggc tatccgcctc cgatcgtgga
     1501 ccacccggcc gagctgcctg cggagccccg cgcgcgttca cgcgtggttc agcagcatca
     1561 gctccttcgg gtgcggattc aggtagacct gcgccgcgag gtgggcgagg ccgagccggc
     1621 gggcgtggtg acggatcagg gtgatcggga ccacgcgcgg caccaggccg cgccggtagt
     1681 ccgcgatggt ctcggccagc tccgcgcgct cctcggcggt cacgtcgcgc ttgacgtaac
     1741 cggccaggtg cgcgagcacg ttggcgtggc ggccgcgcgt ggcgcgcacg gcgagggctt
     1801 ccaggagcag cgcgccgtac tcgtcgagcg tggcgcgcgg aagcgcgggg cccgcggcgg
     1861 ccacgaggcg gccgagccgc gagtagcgcg cggggctgtg agcgagcacc gcgtacttgt
     1921 gggcggtgtg gaaggccacg aggtcgcgcg gccgcggggc gcccgcccgg aagtcccgcc
     1981 agcgggccgt cgcgaacacg cgcccgatga agtgctcccg catcacgggg tcggtgagcc
     2041 gcccctcctc ctcgaccggg agtgacggca gccggcgcgc gagcgcgtcc gcgtagaggc
     2101 cccggcccac ccgaccgggc tgaccgtcct cgctgtagac gcggacgcgg aagagcccgc
     2161 acgacgggga cccgcgcttg aggacgtagc cgtcgagccc gaggcgctcg agctcggtca
     2221 cgcgcgcctc cgcccagctc cgcatccggc gcgacaggtc ctcgccggtc ttctccacga
     2281 ccagccgcgg taccgcttcg gcgcccacga ggcggagcgt cgggcgcggc accccgaggc
     2341 cgatctcgac ctcgggacac acgggcaccc actcgacgaa cgggccgagc gtgtcggcca
     2401 ggaaggcgtc acgcttgtgg ccgccgtcgt agcggacacg ctcccccagc aggcacgcgg
     2461 ataccccgag ccggatcttt tcgggctcga tttttcccat cggctcgcat agtgtagcaa
     2521 ccgtgctcga gccgctcagc cggacgcctt ccctgagccc gaccgcgcag agagtgcagg
     2581 acgtgctcac ggccggcggc tatccctacc aggtcgtcga gtcgtcggtg tccacgcgca
     2641 ccgcggccga ggccgcgaag ctggtgggct gcgaggcggg acagatcgcc aagtcgctcg
     2701 tgttcaagac cgagaagacc gggcgcgccg tgctcgtgat cgccagcggc gtcaaccagg
     2761 tgaacgagtt ccgcatcggg atgtacctga aggaagcggt cgtcaaggca ccggcctcgc
     2821 tggtgcgcca ggccacgggc ttcgccatcg gcggcgtgcc gccgctgggc cacgcaacgg
     2881 cgatggagac cttcatcgac caggacctga tgaagcacgc cgagatctgg gcggccgggg
     2941 gcacgcccaa cgccctcttc aagctcgtgc cggccgactt gacgaagatg tccggtggtc
     3001 gcgtggtgaa ggtgacgtaa gtgccgcgga tcggggcgga acggggcgcc ggcggtgctt
     3061 gaggccgtca ccatgaagga gatcgacgcc atcttcgccg tcaccgacgc gctcgggatc
     3121 caccgcgagc ggctcacgat cccgctcggc ccggcctcgc cgggccgcgt ccggcgcctg
     3181 ccgagcggca agctcgagat cgtcgtcgag gcccggcgcc cgctggacga gtggctgaag
     3241 gagttgcccg cgctgatcgc ggccgccggg aggacgtgaa cgcggggctc cggcgcgagc
     3301 tcggcttcgt ccgcctcgtc gccgtcgtct tctcaacgtc agcggcggtc cctgcggcat
     3361 cgaggacgtg gtgccggcct tcgggccggg ctctcgctgc cgcttctcgc tggcgccggt
     3421 cctctggaac ctgccggtct cgccggccat gtcggaactg ggctcggcca tgcgcctgca
     3481 tcctgccggt ggcggtcggg ctcggcagcg cagcgcgccg tcggctggca agggacacgg
     3541 gcatactgcc cgtggtcggc ggcgccgtcg gggggggccg cgtcgaggcc gggggctgtc
     3601 ggcgtgcgtc gcgccggatc gggtccgcgt cgcacgcgct ggagggcgcg gtgctcgagg
     3661 cctggacggc gacgggaatc ttggcccgac ctcgcgtcga gcgtgatccg agggccgagg
     3721 cggtgaatcg gaggagcacg gcacgatgag cggtgtcgtg aactgcgcgg cctatgccgg
     3781 cggccggaag gtggccgacg tggccgtgga cgacatcagc gaggtgctca agcggcccga
     3841 cgtcttcgtg tggatcggcc tgcacgagcc ggacgagccg ctgctgcgcc gggtgcaggc
     3901 ggaattcggg ctgcacgacc tcgccatcga ggatgctctc cgggcgcacc agcggcccaa
     3961 gctcgagcgc tacggcgact ccctcttcgt ggtgctgcgg accgcggtga tcgacggcgg
     4021 cgaccggcgc atcgccttcg gggagacgca catcttcgtg ggcgagcgct acgtcgtcgc
     4081 cctccgccac ggcgccacgc actcctacac cgacgtccgt gcccggtgcg aggcggcgcc
     4141 catgcagctc gccaagggtc cgggcttcgt gctgtacgcg ctgatggact tcatcgtcga
     4201 ccagtacttc cccgtggccg acctcctcga ggacgagctc gagacgctgg aggaaaacgt
     4261 cttcggcgag gccttcagtc gcgagaccac ggcgcgcatc taccggctca agcgcgacct
     4321 gctcgacgtc aagcgcgcgg tgtcgccgct cgtggacgtc tgcaatcgcc tcatgcgctt
     4381 cgacctcgag ctgatcccgg aggacacgcg actgtacttc cgggacgtct acgaccacgt
     4441 gatccgcatc aacgagatgg tcgacatgct gcgggagctg ctgaccaccg cgctggaggc
     4501 gaacctgtcg ctgatctcgg tctcgcagaa cgacgccatg aagcggctcg cgggctgggc
     4561 cgcgatcatc gccgtcccca cgatggtcgc gggcatctac gggatgaact ttcgtttcat
     4621 gccggaactc tcctggcgct ggggctaccc cacggcgatg gcggtcatgg tcggcacgtg
     4681 cggctttctc tattacaagt tcaagcggtc ggggtggcta tagcaatcgg aggggcctcg
     4741 gcgggcgcgg tggagcgatg tcctggcggc ccacgcgagg gcgtggcaac gggcggccgc
     4801 cggcagccgg cgggcacgtc gactcgagcg gcgcacgctc agcggggcgc gagattctcg
     4861 tcgatgatcg tcgcgaggca atcgaggcgg gcgatcgacg cgacgtagct ccgccagccg
     4921 atcgccacgt ggtcctcggg ctcccccggg cgtgggacag ggtggatggc cacgctcacg
     4981 ttcggcccct gtcccgcaca gccgcccgca cacgcccgcc gcacgcgcac atggtgctcg
     5041 agaccccgct cgccggccat ccgctcgagc aggtcggcga tcgcccgcgc gtcgagccgc
     5101 cgcgcccgct ctccgggtcg gacgggcagc ctcacgctcc cgggctcggt cgggcagacg
     5161 gtgatgacga gacggcgccg gagggtcacg cgcgccgcgc cgtgcctgga ggagcggccg
     5221 gcgggcgcgc gcccgccgcg agctgggccg ccagccgcgc cgccgcgacc acggccggcg
     5281 ctcgcggcgt ccgccagcgc gccgggggac ctgtgcgagt cagggcgcga cgcacgccgg
     5341 ccctgaacca cgcatcggcg agtagcccgc cggcccaggt gacggtgacc ggcgccggca
     5401 gcgcgagctc acgcaccacg ctggccgcca gccgggccag gtggcgctgc gcctcgcgca
     5461 cgatcgcccg cgcgcggcga tcgcccgcgc gcgcggcgcg cagcacggct ggcgcgcggg
     5521 cggcgatgcg cgcgatcgcg tcgggggtgc gcgcgatcct ccgggccggg ggggcgccgt
     5581 cgtcgaccgt ggcccgaatc cactcgtggc cgatccagaa cgccgagccc tcgtccccca
     5641 gcagcgggcc gagcccgccg gcgcgccgcc agcggcccag cgcgtcgtga ccgatcgcga
     5701 tggagccggt gccggcgagc aggagcacgc cgggccggcc gctcagcgcg ccgagcgccg
     5761 cggcctcggc gtcggagagc gcgagcacgc gccgcgccag cggcgcgagg cgtcgcgcca
     5821 gcgcgcggcg ctcgccggcg gtccacacgc cccgcgacgc gatgacgagg gccgcgacgt
     5881 cgcgccggcg ccagccacgt tggttccaca ctgcgcgcaa cgcgtcgatc atcctcgccg
     5941 gcggtggtgc ggttcggcgc cacgtcgcga gccggcgtgg gccggccgtg gcatgcagac
     6001 gcatccacgt gcctccgaga tcgacgccca cagcgaccgc catgtcgtgt ctattcgcag
     6061 gcatagccgt gacttcctgt cctcgattgt cccgcaatcg atccgcagcg acgcccctga
     6121 aatcagacgc ttaaccgggt ggcccgccgt ggcatgggtc ctgctgccat cccgccggcg
     6181 gggggctgaa cgatgggcaa ggcactggcg acgtggctcc tggtctcggc ggccgcggcg
     6241 tcgtccgcgg tgacgcctcg cgacgtcgtg cagtccgcgg tcgtccgggt cgtccaggcc
     6301 atcgaggagt ctcacctcgc gcggcccgag cccccggcca agccggtcgg cggcaagctt
     6361 cggctcgaga tccgccgcgt cgcggccgag ctgttcgact tcgaggagat ctcccgccgg
     6421 gccctctcac gccactgggc gggccggtcg tccgccgagc aggccgagtt cgtcgcgctc
     6481 ttcaccgacc tcctcgagcg cgcatacttc gggcgcatcg aagcgtattc gggcgagaag
     6541 atcgtgtaca ccggcgaggc cgtggacggc gactacgcga ccgtgcgctc gcgcatcctc
     6601 acgcggcggc ggacggagac cgccctcgac taccgcatgc acaagatcgc cgg
//
LOCUS       DATIAZ010000208         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_584974, whole genome shotgun sequence.
ACCESSION   DATIAZ010000208 DATIAZ010000000
VERSION     DATIAZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584974"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VLF19_07480"
     CDS             <1..798
                     /locus_tag="VLF19_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSE93131.1"
                     /translation="GYVLTNFHVVKGADAVTVRLSNKREYRGRIVGSDPKTDLAVIRF
                     EPDGELTTAPLGNSDALRVGEWAIAIGNPFGLDQTVTVGVVSATGRADVGIASYENFI
                     QTDASINPGNSGGPLVNLRGEVIGINTAIVATGQGIGFAIPANMAKRITTQLMDRGKV
                     TRGWIGVSLQPLTTELAQALGLSGTRGAIVGRVYPGSPASGAELKQNDVITNFDGIEV
                     EDYHHLQRLAAEAEVGRSVKLGIVRDRKPRTVELKVAEAPDAPGTGR"
     gene            complement(1000..1827)
                     /locus_tag="VLF19_07485"
     CDS             complement(1000..1827)
                     /locus_tag="VLF19_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HSE93132.1"
                     /translation="MAIVTISHQMGSSGPDVGMAVGQRLGYRYVDQELLQDAVRRYGL
                     VEEKLSHLDESKPTLFERFDAETRHYITILQTTLLEFAEVDNVVLMGRGGQWLLRGIP
                     HVLRVRVIAPFEHRVKQWIRRTAEMTGETPNHRAAADFVRRDDAEKKGRMRYLYEVDI
                     DDPGLYDLVVSTDKLPQDAVVDMIERVARHPALATTEAGRQVVTARALASRVQVALAM
                     HPDTRRYRITVEALGGVVTLEGTAALDQAVQVARDVPGVREVRTRQVEIPPIPPFVA"
     gene            complement(1831..2028)
                     /locus_tag="VLF19_07490"
     CDS             complement(1831..2028)
                     /locus_tag="VLF19_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93133.1"
                     /translation="MARLSRAEVEHLMTERPGSTLEAALDVFEVFASGSLTDEVYVLD
                     DVAGKRIAIAPTALKDKYRRG"
     gene            complement(2130..2795)
                     /locus_tag="VLF19_07495"
     CDS             complement(2130..2795)
                     /locus_tag="VLF19_07495"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HSE93134.1"
                     /translation="MGPLIRAAQQLRYLYAVADGVPRAWRPPREGVDGASVIAIRIGT
                     LVLLASPVAAPPRVTPSLAARHQDIVASLLEARAVLPFAPGTTVGEADLGAWVECRLA
                     RVREALDEMAGHVEMTIRLLRLDAPPGHDRQSGERLRGVADSLVERAGLPHWRYRPSA
                     RDGWAASLAFLVPRGDVPAFLAHIAPVAARAEGLAVVPTGPSAPWSFAPSLDEAPLAA
                     RAG"
     gene            2921..>3128
                     /locus_tag="VLF19_07500"
     CDS             2921..>3128
                     /locus_tag="VLF19_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93135.1"
                     /translation="MSTSSGRPARPLAPVVALILVLSVLSGASPARPAARLPADLPQG
                     QRARCLAIAEAAAVATRVDAEPFVV"
BASE COUNT          456 a         1131 c         1110 g          431 t
ORIGIN      
        1 ggttacgtgc tcacgaactt ccacgtcgtc aagggcgccg acgccgtcac cgtccggctc
       61 agcaacaagc gggaatatcg cggccgcatc gtcggcagcg atccgaagac cgacctcgcg
      121 gtcatccggt tcgagcccga cggcgagctg accaccgccc cgctcggtaa ctccgacgcg
      181 ctcagggtcg gagagtgggc gattgccatc ggcaacccct tcggtctcga tcagaccgtc
      241 acggtcggcg tggtgagcgc caccggccgc gccgacgtcg gcatcgcgag ctacgagaac
      301 ttcatccaga ccgacgcctc catcaatccc ggcaactcgg gcggcccgct cgtgaacctg
      361 cgcggagagg tgatcggcat caacacggcg atcgtcgcca ccggccaggg catcgggttc
      421 gccattccgg ccaacatggc caagcgcatc acgacgcagc tcatggaccg cggcaaggtg
      481 acgcgcggct ggatcggtgt gtcgctgcag cccctcacga cggagctcgc ccaggccctg
      541 gggctcagcg gcacgcgcgg cgcgatcgtg ggccgcgtgt acccgggcag cccggcctcc
      601 ggagcggagc tcaagcagaa cgacgtgatc accaacttcg acggcatcga ggtcgaggac
      661 tatcaccacc tgcaacggct cgcggcggag gccgaggtcg ggcgcagcgt caagctcggc
      721 atcgtccgcg accgcaagcc gcgcaccgtc gagctgaagg tcgccgaagc gcccgacgcc
      781 cccggtaccg gccggtagca ccagcccgtc gcgcggggtc gcgtcggggc gaagcgctct
      841 cccggaacat gccctccggc gccatcgaat ctccggaggg caatctcgcc acccattccg
      901 atgaaccggc gcgcctgcag ccgcgatcgg atggaccggc gagcttttcg gcgggcgcgt
      961 cggggggagc ggcactcgct ccccccgacg gcgatcacgt cacgccacga acgggggaat
     1021 gggcgggatc tcgacctggc gcgtgcgcac ctcacgcaca cccggcacgt cgcgggcgac
     1081 ctgcaccgcc tggtcgagcg ccgcggtgcc ttccagcgtc accacaccgc ccagcgcctc
     1141 gacggtgatc cggtagcggc gcgtgtcggg gtgcatggcg agcgccacct gcacgcggga
     1201 ggccagcgcg cgcgcggtca cgacctgacg gccggcctcg gtcgtggcca gcgcggggtg
     1261 ccgcgccacc cgctcgatca tgtccacgac ggcgtcctgg ggcagcttgt ccgtgctgac
     1321 gacgaggtcg tagagcccgg gatcgtcgat gtccacctcg tagagatagc gcatgcgccc
     1381 cttcttctcg gcgtcgtcgc gacgcacgaa gtcggcggcc gcccgatgat tgggtgtctc
     1441 gccggtcatc tcggcggtgc gccggatcca ctgcttcacg cggtgctcga acggcgcgat
     1501 gacccgcacg cgcaggacgt gtgggatgcc acgcagcagc cactgcccgc cgcgccccat
     1561 cagcaccacg ttgtcgacct cggcgaactc gagcaacgtg gtctggagga tggtgatgta
     1621 gtggcgcgtc tcggcgtcga agcgctcgaa cagcgtcggc ttggactcgt cgaggtgcga
     1681 cagcttctcc tcgaccaggc cgtaccgccg cacggcatct tgcagcaact cctggtcgac
     1741 gtagcggtaa ccgaggcgct gccccaccgc catgccgacg tcgggtccgc tggagcccat
     1801 ctggtgcgag atcgtcacga tcgccatgag tcacccccgc cgatatttgt ccttgagcgc
     1861 ggtcggcgcg atcgcgatcc gcttgccggc gacgtcgtcg aggacgtaga cctcgtcggt
     1921 gagcgatccg ctcgcgaaga cttcgaacac gtcgagcgcg gcctcgagcg tcgagccggg
     1981 acgttcggtc atgaggtgct cgacctcggc gcgagagagt cgggccatcg ggctcctcgg
     2041 gtggcccatc agcgtactcg aaagcgtccg gccgggcaag ggctcccgcg agggcggcga
     2101 ggcccgtcct cgggaggccc ggcacgctgc tatccggcgc gggcggccaa gggagcctcg
     2161 tcgaggctcg gcgcgaacga ccagggcgcc gatgggcccg tcggcacgac ggccaggccc
     2221 tcggcgcgcg cggcgaccgg tgcgatgtga gccaggaagg ccgggacgtc gccgcgcggg
     2281 acgaggaacg ccagcgaggc cgcccagccg tcgcgcgcgc tgggccggta ccgccagtgc
     2341 ggcaatcccg cccgctcgac caggctgtcg gccactccgc gcaggcgctc tccggattga
     2401 cggtcgtgcc cgggcggagc gtcgaggcga agaagccgga tcgtcatctc gacgtggccg
     2461 gccatctcgt cgagggcctc gcgcactcgt gcaagccggc actcgaccca ggccccgagg
     2521 tcggcttcac cgaccgtggt tcccggcgcg aacggcagca ccgcccgcgc ctccagcagg
     2581 ctggcgacga tgtcctggtg gcgcgctgcc aggctgggcg tgacacgggg cggcgccgcg
     2641 acggggctgg cgagcaggac cagcgtgccg atccggatgg cgatgactga ggcgccgtcc
     2701 acgccttcgc ggggcgggcg ccaggcgcgc gggacgccgt cggccaccgc gtacaggtag
     2761 cgcagttgct gcgcggcccg gatcaaagga cccatccctg cctcctgcgg cgataagtgc
     2821 gggccggtgc catctgggaa cacgtctgag ggagttgtcc gagagtacga ggcagcggcg
     2881 gcgaggtcaa gcggttccgc ggggggccta taatggcctc gtgtcgacgt cgtccggtcg
     2941 gccggctcgc ccactcgcgc ccgtcgttgc cctgatcctg gtcctctcgg tcctgtccgg
     3001 cgcctccccg gctcgtccgg ccgcgcgcct gccggcggac ctgccccagg ggcagcgcgc
     3061 gcggtgcctg gcgatcgcgg aggccgccgc cgtcgccacc cgcgtggacg ccgagccctt
     3121 cgtcgtcc
//
LOCUS       DATIAZ010000209         2512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1503830, whole genome shotgun sequence.
ACCESSION   DATIAZ010000209 DATIAZ010000000
VERSION     DATIAZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503830"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VLF19_07505"
     CDS             <1..315
                     /locus_tag="VLF19_07505"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSE93136.1"
                     /translation="ETPAPFEHLRASMTGARTDLFEGAPAAGEGEVSGSGCPFAGMLA
                     EGAAPPDTVAWTPEAQARMDRVPEFIRPMARKAIERYAESRGYASVTEQVMDEARGTF
                     GM"
     gene            316..585
                     /locus_tag="VLF19_07510"
     CDS             316..585
                     /locus_tag="VLF19_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSE93137.1"
                     /translation="MTRAGLVAQMAKASGWPKTGTDRALRAMLAAIRGSLKRGDSVTL
                     VGFGTFSVVRRRPRTLRNPRTGQTVTAAGRLPRFKPSKELKQAVR"
     gene            634..1530
                     /locus_tag="VLF19_07515"
     CDS             634..1530
                     /locus_tag="VLF19_07515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSE93138.1"
                     /translation="MRRSVLGAVAVILALLVSAVAVAAQANRVPALNAESVILLDAEG
                     RTIHAKNPDLERAPASLVKLMTLYLAFEDLEAGRADLEQPVTISRYAATTPRYRMGLR
                     TGRQVPLRVLLEGVAIASANDAATAVAEHLAGDETSFVARMNAKARELGLSATHFANP
                     HGLPDPAQRSSARDLAQLTARLLNDHAAARTMLGGQTLIYRGRVYARHVPLFYDPLGV
                     QALKTGFTNEAGYNLAVAAWRNGQQFTMIVLGCRSRARSFLDAKRLLHYGFVEAGLEP
                     PAEEPRRVPRKPVRVRRTFKHR"
     gene            1535..2185
                     /locus_tag="VLF19_07520"
     CDS             1535..2185
                     /locus_tag="VLF19_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HSE93139.1"
                     /translation="MTIRGYVFDAYGTLFDVHSVVEAGRRITADPVALSTTWRQKQLE
                     YTWLRALMGRYEDFWAVTEAALRYAIRRLGLTASDAEVRRLMQAYLSLACFPEVKSAL
                     QRLGSRPCAILSNGSPRMLEAAVAASGLGPHLAHVLSVDAVQTYKPSPRVYELGPRAL
                     GEPAGDLLFVSSNAWDVAGAKAFGYRVAWCNRTNAPEEELGFRPDLVISRLDELPL"
     gene            complement(2329..>2512)
                     /locus_tag="VLF19_07525"
     CDS             complement(2329..>2512)
                     /locus_tag="VLF19_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93140.1"
                     /translation="PRRGGGQMAKAKKAFGGYSISFKGCKDSLESLFGSAPIGPSVMT
                     KKLWGYVKRKKLGKRR"
BASE COUNT          348 a          931 c          848 g          385 t
ORIGIN      
        1 gagacccccg ctccgttcga gcacctccgc gcgtcgatga cgggcgcgcg caccgacctc
       61 ttcgagggcg cgccggcggc cggggagggc gaggtgtccg gatccggctg tccgttcgcg
      121 ggcatgctcg ccgagggcgc ggcaccgccc gacaccgtgg catggacgcc cgaggcccag
      181 gcgcgcatgg accgcgtgcc ggagttcatc cggcccatgg cgcgcaaggc gatcgagcgg
      241 tacgcggagt ccaggggcta cgcgagcgtc accgagcagg tgatggacga agcgcgcggt
      301 accttcggaa tgtagatgac ccgcgccggt ctcgtcgcgc agatggcgaa agcgagcggc
      361 tggcccaaga ccggcaccga tcgggccctt cgggcgatgc tcgcggcgat ccggggctct
      421 ctcaagcgcg gcgactcggt gacgctcgtc ggcttcggca cgttctccgt cgtccgccgg
      481 cgcccccgta ctttgcgcaa cccccggacc ggacagaccg tcacggccgc cgggcggcta
      541 cccaggttca agccgtccaa ggagctgaaa caagcggtcc gctgaggctt tttgcttgcc
      601 gcctccgcgc cgcgctggct agaatcgacg tcagtgcgtc gctccgtcct gggcgcagtg
      661 gccgtcatcc tggcgctcct ggtttccgcc gtcgccgtcg ccgctcaggc gaaccgcgtt
      721 ccggcgttga acgcggagtc cgtcatcctc ctcgacgccg aggggcgcac gatccacgcc
      781 aagaacccgg acctcgagcg cgcgccggcc agcctcgtca agctgatgac gctctacctg
      841 gctttcgagg acctcgaggc cgggcgcgcc gacctggagc agccggtcac gatctcccgc
      901 tacgcggcca ccacgccgcg ctaccggatg ggcctcagga cgggcaggca ggtgccgctg
      961 cgggtgctgc tcgagggcgt ggccatcgcg tccgcgaacg acgcggcgac ggccgtggcc
     1021 gagcacctgg ccggtgacga gacctccttc gtcgcccgca tgaacgccaa ggcgcgcgag
     1081 ctgggcctca gcgccaccca cttcgccaat ccccacgggt tgcccgatcc ggcccagcgc
     1141 agcagcgcgc gggatctggc gcagctcacc gcccggctgc tgaacgatca tgcggccgcg
     1201 cgcaccatgc tgggcggcca gacgctcatc taccgcgggc gggtgtacgc gcgacacgtc
     1261 cctctgttct acgatccgct cggcgtgcag gcgctcaaga cgggcttcac caacgaggcc
     1321 ggctacaacc tggcggtcgc ggcgtggcgc aacggccagc agttcaccat gatcgtgctc
     1381 ggctgccgga gccgggcgcg ctcgttcctc gacgccaagc gcctgctgca ctacggcttc
     1441 gtcgaggccg gcctcgagcc gccggccgag gagccgcgcc gcgtgccccg caagcccgtc
     1501 cgcgtgcggc gcacgttcaa gcaccgctga cgccgtgacc atccgcggct acgtcttcga
     1561 cgcgtacggc acgctcttcg acgtccattc cgtcgtcgag gcgggccgga ggatcacggc
     1621 cgacccggtc gcgctctcga ccacctggcg gcagaagcag ctcgagtaca cgtggctgcg
     1681 tgcgctgatg ggccgttacg aggacttctg ggcggtgacc gaggcggcgc tgcgctacgc
     1741 catccgccgg ctggggctca ccgcgagcga tgccgaggtg cgccggctca tgcaggcgta
     1801 cctctcgctc gcgtgcttcc ccgaggtgaa gtccgcgctg cagcgcctgg gctcacggcc
     1861 gtgcgcgatc ctgtcgaacg ggtcgccgcg catgctcgag gccgcggtgg ccgcgagcgg
     1921 gctcggcccg cacctcgccc acgtgctctc ggtggacgcg gtccagacgt acaagccgtc
     1981 accgcgggtc tacgagctcg ggcccagggc cctcggcgag cccgccggcg acctgctctt
     2041 cgtctcgtcg aacgcgtggg acgtcgcggg cgcgaaggcc ttcggctacc gggtggcctg
     2101 gtgcaaccgc accaacgctc ccgaggagga gctcggtttt cgccccgacc tggtgatcag
     2161 ccgcctcgac gagcttccgc tctgaactca gccgatcatt tcgcgccggg agtcacctga
     2221 aggcccattg cgggttcgat ccccgactgc gcccgcgcga tcgaattgct tgtgacggct
     2281 cggagggggc cgtcgaggcc cccttcgaac aaagactcct ccaaagcctc agcgacgctt
     2341 gccgagcttc ttccgcttca cgtagcccca cagcttcttg gtcatcacgg agggtccgat
     2401 cggcgcgctg ccgaagagcg actccaggct gtccttgcac cccttgaagc tgatcgagta
     2461 cccgccgaac gccttcttcg ccttcgccat ctgtcctcct cctcgccgtg gt
//
LOCUS       DATIAZ010000210         5311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1109788, whole genome shotgun sequence.
ACCESSION   DATIAZ010000210 DATIAZ010000000
VERSION     DATIAZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109788"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VLF19_07530"
     CDS             complement(<1..335)
                     /locus_tag="VLF19_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSE93141.1"
                     /translation="MLRELRIRNFAVIETVTVPFAPGLNVLTGETGAGKSIVIDAILL
                     VRGVRAQADVIRADAESATVEAVFDVAGRREVLAVLADAGLAAEDGAVVVRRELTRSG
                     RHRAFVNDCA"
     gene            complement(458..1315)
                     /locus_tag="VLF19_07535"
     CDS             complement(458..1315)
                     /locus_tag="VLF19_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HSE93142.1"
                     /translation="MKRIGIVAKPDAGAADEVVRRLVQWLGGRGITPVLDKETAALVA
                     DLGPAQARRADVPGQVDAIVVLGGDGTLLSVARAVGDLGIPILGVNLGGLGFLTATTL
                     EEMIPALDAFVGGRLIAEERMMLSARVGRGDETVAEALALNDVVIMKAAMSRIIDLSV
                     SVEGQAATAYRADGLIIATPTGSTAYSLSAGGPILFPTMDAVVLTPICSHTLTNRPIV
                     LPGASRIEVTLHSSQEAMCTLDGQVGFALRDGDVVEVQRARSRIRLARIPQKHFFSVL
                     RTKLKWGER"
     gene            complement(1326..2117)
                     /locus_tag="VLF19_07540"
     CDS             complement(1326..2117)
                     /locus_tag="VLF19_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HSE93143.1"
                     /translation="MATRIRIDRLLAERGLVESREKAAKLVLAGDVFVDGERVDKVGA
                     LVSPEAEVELRGRSPYVSRGGEKLMYALDHFNVPVVDRICIDVGASTGGFTDCLLQRG
                     AARVYAVDVGTGQLDQRLRRDPRVVVMEQTNARALDPRLFADQPSLAVIDVAFISLEK
                     VLPAVFGVLAPRADVVMLIKPQFEVGREHVGKGGVVREPSLHRQVVARLARYAVLRGW
                     HVLGVSASPLRGPKGNREFFLHVCSHGRTASNLESMISRTVEALA"
     gene            complement(2129..2845)
                     /locus_tag="VLF19_07545"
     CDS             complement(2129..2845)
                     /locus_tag="VLF19_07545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSE93144.1"
                     /translation="MLPLRADVASETTPVVTVGLIAANLLVFLYQLSLEMGPDPGAAR
                     AGQAFVLEFGAIPCRFTGACRAAGEFPNPALTALTSMFLHGGLFHVGGNMLYLWIFGN
                     NVEDTLGHVRFLFFYLASGVAAALAQVVMHPASQVPMVGASGAVSGVLGAYLLLFPYA
                     RVLTLIMFGFFFRFAHVPALVVLGFWVVVQFLNGFLTVSLGGAERGSGGGVAWFAHIG
                     GFLAGMALLFVLRRRPRGRL"
     gene            complement(2845..4146)
                     /locus_tag="VLF19_07550"
     CDS             complement(2845..4146)
                     /locus_tag="VLF19_07550"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSE93145.1"
                     /translation="MTPRRLVAALALAALGAGVAAAQPTAAAVSKHVLPNGVRVLIRE
                     EASAGVIAVSLHVLGGSRLETVATSGITNFLHHVMLRGTARRTGEQLAEAAERLGGTV
                     DASSDADHAEVRGTALARHWEDLLGLVAEVALRPTLPAEEVERERRLVLGQIQTRADT
                     PFPLAFDTLLAELYGQHPYGRPSSGRRQSVTGITREVLASHHRALYRADRIVLAVSGR
                     IERERVRRAAERLFGEVPAIDGDQAAAPAAPVASLGRRVVQKPAQQAQVVVGFLGPTV
                     HDADYPAVKVLAALLGGGMSGRLFTELRDRQGLAYSLGVVNPTRAGPSPIVAYVGTAP
                     ENVAAVEPVMLRELARARTEEATTDELARAKAYVLGGFAMDRRTNARHAWYLAFFEGL
                     GVGWDYPGRYVRAVESVTAADVRAAAARYLRAPTTVVLTPR"
     gene            complement(4143..>5311)
                     /locus_tag="VLF19_07555"
     CDS             complement(4143..>5311)
                     /locus_tag="VLF19_07555"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSE93146.1"
                     /translation="VVALQLWVQAGGRDEAAGEVGLAHYLEHMLFKGTATRPAGFIDR
                     EVEGVGGRMNAGTSLDYTYYHMLLPARRALAGIETLADVSVNAALDETQLEREKRVVL
                     EEMRLGKDRPTRFLARQLYEVLFQGHPYGRPVIGRDDLVRALTREQLLAFYRRHYAPE
                     SFTLVVVGAVAPDEVLAVATREFGRLPRIGFSRLPVAPVAEVQPRRVERQRPGAHAYL
                     GLAWLAPRLDHADTPAVDVLASVMGQSRSARLPRTLRDRLGLVNSISASYAALEGAGV
                     LSITAQLDPASLERAQAEIMAEAQRLRRDGITTAELERAITTAEAQREFRMETAEGRA
                     FTLGHAETIWRIEDELAYVDRVRSVSVEQVRAAAQRYLDPERFARVVLTPGGAR"
BASE COUNT          822 a         1909 c         1913 g          667 t
ORIGIN      
        1 gcgcagtcgt tgacgaaggc ccggtgacgt ccggagcgcg tcagctcgcg ccgcacgacc
       61 accgcaccgt cctcggccgc caggccggca tccgccagca cggcgagcac ctcacgccgg
      121 ccggccacgt cgaagacggc ctcgaccgtg gcgctctcgg cgtcggcgcg gatcacgtcg
      181 gcctgcgcgc ggacgccgcg cacgagcagg atcgcgtcga tcacgatgga cttgccggcg
      241 ccggtctcgc cggtgaggac gttcagcccg ggcgcgaaag ggacggtgac ggtctcgatg
      301 acggcgaagt tgcggatgcg aagctctctc agcatcccgc ctggatgcta tgcgagacgg
      361 cgcgaccgct caagccgtat ccgccgcgtg acggcgacgg gtcagacgtc cgcgcgtcga
      421 tggtcgccgc gggccgggcg acggacgaac ccccgggcta ccgttcgccc cacttcagct
      481 tggtgcgcag gacggagaag aagtgcttct ggggaatgcg cgcgagccgg atccgagacc
      541 gggcgcgctg aacctcgacc acgtcgccgt cacgcagcgc gaagccgacc tgtccgtcga
      601 gggtgcacat cgcctcctgg ctagagtgca gggtgacctc gatgcgcgaa gcaccgggca
      661 gcacgatggg ccggttcgtg agcgtgtggg aacagatcgg cgtgaggacc accgcgtcca
      721 tcgtggggaa caggatgggc ccgccggccg agagcgagta agcggtcgag ccggtcggcg
      781 tggcgatgat gagcccgtcg gcacggtaag cggtggccgc ctgcccctcc accgacacgc
      841 tcaggtcgat gatgcggctc atcgccgcct tcatgatgac gacgtcgttc agcgccaggg
      901 cctcggcgac ggtctcgtcg ccgcggccca cccgggccga gagcatcatg cgctcctcgg
      961 cgatgaggcg gccgccgacg aacgcatcga gcgccggaat catctcctcg agggtcgtcg
     1021 cggtgaggaa gccgaggcca ccgaggttca cgccgaggat cgggatgccg agatcgccga
     1081 cggcccgcgc gacggagagc agcgtgccgt cgccgccgag gaccacgatg gcgtcgacct
     1141 ggccgggaac gtccgcgcgg cgcgcctgcg ccgggccgag gtcggcgacc agcgccgcgg
     1201 tctccttgtc gaggacgggc gtgatcccgc gccccccgag ccattgcacc agccgccgta
     1261 cgacctcgtc cgccgcgccg gcgtccggct tggcgacgat cccgatccgc ttcacgtggc
     1321 gctcgtcagg ccagcgcctc gaccgtccgg ctgatcatcg actcgagatt cgatgcggtg
     1381 cggccgtggg agcagacgtg gaggaagaac tcgcggttgc ccttgggccc gcgaaggggc
     1441 gacgccgaga ccccgagcac gtgccacccg cgcaagacgg cgtacctggc gaggcgcgcg
     1501 accacctgcc ggtgcagcga gggctcgcgc acgacgcccc ccttgccgac gtgctcgcgc
     1561 ccgacctcga actgcggctt gatgagcatg acgacgtccg cgcggggcgc gaggacgccg
     1621 aagaccgcgg gtagcacctt ctccagcgag atgaacgcga cgtcgatcac cgcgagcgac
     1681 ggctggtcgg cgaacagccg cgggtccagc gcgcgggcgt tcgtctgctc catcaccacc
     1741 actcgcgggt cgcggcgcag gcgctggtcg agctgtccgg tgccgacgtc caccgcgtac
     1801 acgcgcgcgg caccgcgctg cagcaggcag tcggtgaagc cgcccgtgga cgcgcccacg
     1861 tcgatgcaga tacggtcgac gacgggcacg ttgaagtggt ccagcgcgta catcagcttc
     1921 tcgccgccgc ggctgacgta gggcgagcgt ccccgcagct cgacctcggc ctcgggggaa
     1981 acgagggcgc cgaccttgtc cacccgctcg ccgtccacga acacgtcgcc ggccagaacc
     2041 agcttggcgg ccttctcccg gctttcgacg agaccccgtt ccgcgagcaa ccgatcgatc
     2101 cggatccgtg tggccatggg cgacctcatt ataggcgacc gcgcgggcgc cgccgcaaga
     2161 caaagagcag cgccatccct gcgaggaagc cgccgatgtg ggcgaaccag gcgacgccgc
     2221 cgccggaccc gcgctccgcg ccgcccaggc tcacggtcag gaagccgttg aggaactgca
     2281 cgacgaccca gaagccgagc acgaccaggg ccggaacgtg cgcgaaccgg aagaagaacc
     2341 cgaacatgat gagggtcagc actcgcgcgt acggaaacag cagcaggtag gcgccgagaa
     2401 ccccggacac ggcaccactg gccccgacca tcggcacctg ggaggcggga tgcatcacga
     2461 cctgcgcgag cgccgcggcg acacccgaag cgaggtagaa gaacaggaaa cgaacgtggc
     2521 cgagcgtgtc ttccacgttg ttgccgaaga tccacaggta cagcatgttg ccgccgacgt
     2581 ggaacagccc gccgtgcagg aacatcgagg tgagggcggt cagggccggg ttcgggaact
     2641 cgcccgcggc gcggcaagcg ccggtgaacc ggcacgggat ggcgccgaac tcgaggacga
     2701 acgcctgccc ggcgcgcgcg gctccgggat cggggcccat ctcgagcgag agctggtaga
     2761 gaaagacgag gaggttggcg gcgatcagtc ctaccgtcac gaccggcgtg gtctcgctcg
     2821 cgacatcggc cctgaggggc agcatcagcg gggtgtgagc acgaccgtgg tcggcgcgcg
     2881 gaggtaacgg gcggcggcgg ctcgcacgtc ggccgcggtc accgactcga cggcgcgaac
     2941 gtaccgcccc gggtagtccc agcccacgcc gaggccctcg aagaacgcca ggtaccacgc
     3001 gtgacgcgcg ttggtgcgcc ggtccatggc gaagccgccg agcacgtagg cctttgctcg
     3061 cgccagctcg tcggtcgtcg cctcctcggt gcgcgcgcgc gccagctcgc gcagcatgac
     3121 gggctcgacc gccgcgacgt tctcgggcgc ggtgccgacg tacgccacga tcggcgacgg
     3181 gccggcacgc gtggggttca cgacgcccag ggagtacgcc aggccctggc ggtctctcag
     3241 ctcggtgaag agccgtcccg acataccgcc gccgagcagc gcggcgagca ccttcaccgc
     3301 ggggtagtcg gcgtcgtgga cggtgggccc caggaaaccg acgacgactt gcgcctgctg
     3361 ggcgggcttc tgcacgaccc ggcgccccag cgacgcgacg ggcgccgccg gcgcggccgc
     3421 ctggtcgccg tcgatggcag ggacctcgcc gaagagccgc tccgccgccc ggcgcacccg
     3481 ctcacgctcg atccggccgc tgacggccag cacgatgcgg tcggcccggt agagcgcgcg
     3541 atgatgcgac gccagcacct cccgcgtgat gcccgtcacg ctctggcgcc ggccgctgct
     3601 cggccgcccg tagggatgct ggccgtacag ctcggcgagc agcgtgtcga acgcgagcgg
     3661 aaagggcgtg tcggcgcgcg tctggatctg tccgaggacg agacgccgct cgcgctcgac
     3721 ctcctcggct ggcagcgtcg gtcgcaacgc cacctctgcg acgaggccga gcaggtcctc
     3781 ccagtggcgc gccagggcgg tcccccgcac ctcggcgtgg tcggcgtcgc tcgaggcatc
     3841 cacggtgccg ccgagccgct cggcagcctc cgccagctgc tcaccggtac ggcgcgccgt
     3901 gccccgcagc atcacgtggt ggaggaagtt cgtgatcccc gacgtcgcga cggtctcgag
     3961 ccgcgatccg cccagcacgt gcaacgagac ggcgatgacg cccgcgctcg cctcctcgcg
     4021 gatgagcaca cgcacgccgt tgggcagcac gtgcttgctc accgccgccg ccgtcggctg
     4081 cgcggccgcg acaccggcgc cgagtgccgc cagcgcgagc gcggcgacga ggcggcgcgg
     4141 cgtcatcgcg cgccccccgg cgtcagcacg acgcgggcga accgctcggg gtccaggtag
     4201 cgctgggcgg cggcgcggac ctgctcgacc gacaccgagc gcacgcgatc cacgtacgcc
     4261 agctcgtcct cgatccgcca gatcgtctcg gcgtggccca gcgtgaaagc tcggccctcg
     4321 gccgtctcca tccggaactc gcgctgcgcc tcggccgtgg tgatggcgcg ctccagctcc
     4381 gccgtcgtga tgccatcgcg acgaaggcgc tgagcctcgg ccatgatctc cgcctgggcg
     4441 cgctcgagac ttgcgggatc gagctgcgcg gtgatgctga gcacgccggc gccctcgagg
     4501 gcggcgtagc tggcggagat cgagttcacg agcccgagtc ggtctctgag cgtccgcggc
     4561 agccgtgcgg agcgactctg ccccatgacg gacgcgagca cgtccacggc gggagtgtcg
     4621 gcgtggtcca ggcgtggcgc gagccaggcg agccccaggt acgcgtgcgc gcccggacgc
     4681 tgccgctcga cccgccgggg ctgcacctcg gcgacggggg ccaccggcag gcgcgagaac
     4741 ccgatccgcg gcagccgacc gaactcccga gtcgcgacgg cgagcacctc gtcgggagcg
     4801 acggcgccga cgaccaccag cgtgaaggac tcgggcgcgt aatgccgccg gtagaaggcc
     4861 aggagctgct ccctcgtcag ggcgcggacc aggtcgtcgc gcccgatcac cggccggccg
     4921 tagggatgcc cctggaacag gacctcgtag agctggcgtg cgagaaagcg agtgggcctg
     4981 tccttgccga gccgcatctc ttcgaggacg acgcgcttct cgcgctcgag ctgtgtctcg
     5041 tccagtgcgg cgttcacgct cacgtcggcg agcgtctcga tgccagcgag cgcgcgtcga
     5101 gccggaagca gcatgtggta ataggtgtag tcgagcgagg tcccggcatt catccggccc
     5161 cccacgccct cgacctcacg gtcgatgaag ccggccgggc gggtggcggt gcccttgaag
     5221 agcatgtgct cgaggtagtg ggccagtccg acctcgccgg ccgcctcgtc ccggcccccg
     5281 gcttgcaccc acagctgcag cgcgacgacg t
//
LOCUS       DATIAZ010000211         6426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1241017, whole genome shotgun sequence.
ACCESSION   DATIAZ010000211 DATIAZ010000000
VERSION     DATIAZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241017"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VLF19_07560"
     CDS             complement(<1..203)
                     /locus_tag="VLF19_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HSE93147.1"
                     /translation="MSPLRIGVVFGGRSGEHEVSLASAASVVVALERAGHDVVPIGIA
                     RDGRWIVGGDPLRALAAEARIGLP"
     gene            complement(233..667)
                     /locus_tag="VLF19_07565"
     CDS             complement(233..667)
                     /locus_tag="VLF19_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93148.1"
                     /translation="MPTTWSWRHWAFFGLALIAVAGYFELITRARVEHVLERLALQSE
                     SAGTVFGQDAGRAEALFIVFAFLFLTPVVFAMGAFVPLFASAVAAEFMFSITRLPQAA
                     GALLFWIALTVTAWMKVDAWLPGVQWLGSLIARAFLIALRAR"
     gene            complement(725..2092)
                     /locus_tag="VLF19_07570"
     CDS             complement(725..2092)
                     /locus_tag="VLF19_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSE93149.1"
                     /translation="MTDTPDLFGSAARPAPARAPVPAGPLADRLRPRALDEIVGQEHL
                     LGPGRVLRAAIERGELHSMILWGPPGSGKTTLASLMAHVAGARFVAFSAVLSGVKEIR
                     QVIAEAEAERRRGGRPTILFVDEIHRFNRAQQDAFLPHVEKGTIVLIGATTENPSFEV
                     NSALLSRCRVYVLRGLGEDELAAIMQRALGDAERGLGGRRPAVDDDALRLIARLAGGD
                     ARSALNILELAVGMAPVEAGRARVTEAAIREAAQRKTLLYDKAGEEHYNLISALHKSL
                     RDSDPDAALYWMARMLDSGEDPLYLARRLVRFASEDVGNADPAALPLALAARDAYDFL
                     GSPEGELALAQATLYLALAPKSNAVYTALDAAREDVRERPAEPVPPHIRNAPTGLMRE
                     LGYGRGYQYAHDAPDARVDQEHLPEALRGREYYRPTDRGLEAELGRRLAEWKRWRLAR
                     RGEAG"
     gene            complement(2163..2585)
                     /locus_tag="VLF19_07575"
     CDS             complement(2163..2585)
                     /locus_tag="VLF19_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93150.1"
                     /translation="MITRRRAGLVLASVVLAVVGSGTSAGQADLRQVEIRVTERHGVI
                     KFQFYRQEHPGTSAMVPAKAHRLMVLRADESPASWMIEAPRDGGALEVTYGDLPEGFR
                     QGIPNDDFAPPLEPHTTYVVSVIFVAGYGSTTFRYSGR"
     gene            complement(2582..3253)
                     /locus_tag="VLF19_07580"
     CDS             complement(2582..3253)
                     /locus_tag="VLF19_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSE93151.1"
                     /translation="MRLCVFDLDHTLIRSPLDLAAMALDMRALLESRQGRLPPREERW
                     RVGELIAHCRREAPALESLLWAVALDHERRAVAAADLEPGAREAVAGARAAGLATAVW
                     TNNAREVAVAALGRVGLLDAFDLVVTRNEMHALKPDPDGWRVIREHFGPCAAVVVGDS
                     WVDGLAATSAGVPFVAYRASPADLARWGITPIASLEDLAALPALLGELNGAPRGGPAA
                     RIGPR"
     gene            3291..4757
                     /locus_tag="VLF19_07585"
     CDS             3291..4757
                     /locus_tag="VLF19_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93152.1"
                     /translation="MKRRVLLVTAFASLLHVASATPPASALDEADRLWMVGDRASADG
                     LPKVARRALERFVAEHPTDPRLPDAVLLLGRARLATGDAETALEAFRRAQGFTPPPGR
                     PLEVKFWEAEALFRLKRFAEARAAYDAVVRTDAASPLAPDAVYGLAWSESELGHAETA
                     VDAFRQFLQTWPDHATAPSATFHLARVLVELKRFKEALPLLEEFTGRYRTHAQAPDAR
                     YLLGLARIETGDTRGGVADLRAFAEAHPTHPQTRAARQLVTRTLARHGNRSELGEVYK
                     TLMAESPATPDGLYDAASVAGRLGRAKEQEAALRRLRGEFPDHALSRRAALELASVAY
                     KRKDWKDTVAQAEAATKSGESAVRAEAWLLVGEGELKLKRFQNAEKAFEAAGAVKEAK
                     PAIRFRALAGLGLAHEEQREWKPALTAYEAVADKSPDATLRDWARERATAVKERMASA
                     PPDTKPPEQKAPEKTPAVKTPPAKTPAEKKPTERKAGS"
     gene            4757..>6426
                     /locus_tag="VLF19_07590"
     CDS             4757..>6426
                     /locus_tag="VLF19_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93153.1"
                     /translation="MRRVVVLALVWGASVAATPIAPTLPPPDLAPLVPWAVAPLDKPA
                     VELPKLALPPSPADVPTVAPATVALPEAQKPTESLPSARTLPCVGAWLRIASESLECG
                     RAKLGRGELDEAARALEHAARPGPEPDVIAEARYWHAETLFRLGQFARADWLFRQVVT
                     DPARTGLRPWAIFGSGFTALRLGDAPRAQEAFRRVLGVAHPAALDGWARHGLALALYA
                     QSRWQDADKTWTDATSRRLPPALERDVLFWHGDTLGRVRQPPRAVEKLASFAQGGPHP
                     LLTPGLVRLGWWSLAAGAPAEALKALRTALVSAPTAGPEVREREWAEAGLALALGATG
                     DLPGARASLAPLDGRRSALALPVRLRLIDAAVESGQPAEALALAQDLLSGNLTPPVRA
                     WVLLMKGEAHRTEGNRDEARTQFDLARDGTPRGDVGRHAALRIAQTNFELREFSQVVA
                     DTGPLLGAAVAPEMRAAALLVQGEAAYHAGNYSVAADSYRRLLVEFPDHEQATAVRLA
                     LAWTVLRQGQRDAARQQFLEFARAHPTNAHAVDALVLASELHLAGGDLP"
BASE COUNT          861 a         2427 c         2359 g          779 t
ORIGIN      
        1 gggagcccga tccgggcctc ggcggcgagc gctcgcagcg gatcgccgcc gacgatccag
       61 cggccgtcgc gcgcgatgcc gatgggcacg acgtcgtggc cggcgcgctc gagcgcgacg
      121 acgacggagg ccgcggaggc cagcgacacc tcgtgctctc cggagcggcc gccgaagacg
      181 acgccgatcc gcagcggcga catggtgcga ttctagcagg cgcgcgcggc ggtcaccgcg
      241 cgcgcagcgc gatgaggaac gcgcgggcga tgaggcttcc gagccactgg acgcccggga
      301 gccaggcatc caccttcatc cacgccgtga ccgtgagggc gatccagaac agcagcgcgc
      361 ccgcggcctg cggcagacgg gtgatcgaga acatgaactc ggcggcgacc gcggaggcga
      421 acagggggac gaaggcgccc atcgcgaaca ccaccggcgt gaggaagagg aaggcgaaca
      481 cgatgaacag cgcctcggcc cggcctgcgt cctgtccgaa gaccgtcccc gcgctctcgg
      541 actgcaaggc cagccgctcg agcacgtgct cgacgcgcgc acgcgtgatg agctcgaagt
      601 acccggccac ggcgatgaga gccaggccga agaaggccca gtgacgccag ctccaggtgg
      661 tcggcacccg cgcacccccc ttaagccgtt ataccccggg gggccgtggg gcagaaaatt
      721 tcgctcagcc cgcctcgccc cggcgcgcga ggcgccaccg cttccattcg gccagccggc
      781 gccccagctc cgcctccagg ccccggtcgg tcggccggta gtactcgcgc ccgcgcagcg
      841 cctccggcag gtgctcctgg tccacgcgcg cgtcgggcgc atcgtgggcg tactgatagc
      901 cgcgcccgta gccgagctcg cgcatgaggc ccgtcggcgc attccggatg tgcggcggca
      961 cgggctcggc cggcctctcg cggacgtcct cgcgcgcggc atccaacgcg gtgtagacgg
     1021 cgttcgactt cggcgccagc gccaggtaga gcgtcgcctg cgccagcgcc agctcgccct
     1081 cgggcgatcc gaggaagtcg taggcgtccc gggcggcgag cgcgagcggg agcgctgcgg
     1141 ggtcggcgtt gccgacgtcc tcggaggcga agcgcaccag gcggcgggcg aggtagagcg
     1201 gatcctcgcc ggagtcgagc atgcgggcca tccagtagag cgccgcgtcg ggatcggagt
     1261 cacgcagcga cttgtggagc gcggagatca ggttgtagtg ctcctccccg gccttgtcgt
     1321 agaggagcgt cttccgctgc gccgcctcgc ggatggccgc ctcggtcacg cgcgcgcggc
     1381 ccgcctcgac gggcgccatc ccgacggcga gctccaggat gttcagcgcg ctgcgcgcgt
     1441 cgcccccggc caggcgcgcg atgaggcgca gcgcgtcgtc gtccaccgcc ggccggcgcc
     1501 cgccgaggcc gcgctcggcg tcccccagcg cgcgctgcat gatcgcggcg agctcgtcct
     1561 ccccgaggcc gcgcaggacg tagacgcggc aacgcgagag cagcgccgag ttcacctcga
     1621 aggacgggtt ctcggtcgtg gcgccgatca gcacgatcgt gcccttctcg acgtggggca
     1681 ggaacgcgtc ctgctgggcc cggttgaagc ggtggatctc gtccacgaag aggatcgtcg
     1741 gccgcccgcc ccgccggcgc tcggcctccg cctcggcgat gacctgccgg atctccttca
     1801 cgccggagag caccgccgag aacgcgacga agcgggcccc ggcgacgtgc gccatcagcg
     1861 aggccagcgt cgtcttcccg gagcccggcg gcccccacag gatcatcgag tgcagctcgc
     1921 cgcgctcgat cgccgcgcgc aggacgcggc cggggccgag caggtgctcc tggccgacga
     1981 tctcgtccag cgcgcggggc cgcaggcggt cggccagcgg cccggccggt accggagccc
     2041 gcgccggcgc gggccgagcg gcgctcccga acagatcggg ggtgtccgtc atttcagggc
     2101 ccggtcattc caccgctcgg ctcgccgcgc ggcgcggctc gccttccgct gccacgcggc
     2161 gatcaccgac ccgagtatcg aaacgtcgtc gacccgtacc cggcgacgaa gatgacgctg
     2221 accacgtacg tcgtgtgggg ttccagcggc ggggcgaaat cgtcgttcgg gatgccctgc
     2281 cggaatcctt ccggcaggtc gccatacgtg acctcgaggg caccgccgtc tcgcggggcc
     2341 tcgatcatcc agctggccgg cgactcgtcc gcccgcagca ccatcagccg gtgggccttc
     2401 gccggcacca tcgcgctcgt cccgggatgc tcctggcggt agaactggaa cttgatcacg
     2461 ccgtggcgtt ccgtcacccg gatctcgacc tggcggaggt cggcctgtcc cgcgctcgtg
     2521 ccacttccca ccacggcgag gaccacgctc gcgaggacga ggccggcccg acgcctcgtg
     2581 atcatcgcgg gccgattcga gccgccggcc caccccgcgg ggcgccgttc agctcgccga
     2641 gcagcgccgg cagcgccgcg aggtcctcga gcgaggcgat cggcgtgatc ccccagcgcg
     2701 cgaggtcggc cgggctggcg cggtacgcga cgaacggcac gccggccgac gtggcagcga
     2761 ggccgtccac ccacgagtcg cccacgacga ccgccgcgca gggaccgaag tgctcgcgga
     2821 tcacccgcca gccgtcgggg tcgggcttga gggcgtgcat ctcgttgcgc gtgacgacga
     2881 ggtcgaacgc gtcgagcagg ccgacgcgcc cgagcgcggc caccgccacc tcgcgcgcgt
     2941 tgttcgtcca cacggccgtc gccaatccag ccgcgcgcgc gccggcgacg gcctcgcgcg
     3001 cgccgggctc gaggtcggcc gcggcgacgg cgcggcgctc gtggtcgagc gccaccgccc
     3061 acaggaggga ctccagggcc ggggcttccc gccggcagtg ggcgatcagc tccccgacgc
     3121 gccagcgctc ctcgcggggc ggcagccggc cctgccgcga ctccagcagc gcgcgcatgt
     3181 cgagcgccat cgccgcgagg tccaggggcg agcggatcag cgtgtggtcg agatcgaaga
     3241 cgcagagacg caccgtacct aggataccgt gctacgatgg tcgcgtcatc atgaagcgcc
     3301 gcgtgttgct cgtcacggcg tttgcctcgc tcctccacgt agcgtccgcg acgccaccgg
     3361 cctccgcgct cgacgaggcc gaccggctat ggatggtcgg cgatcgcgcc tcggccgacg
     3421 gactgccgaa ggtggcccgg cgggcgctcg agcgcttcgt cgccgaacat cccaccgacc
     3481 cccgcctgcc cgacgccgtg ctgctgctcg ggcgcgcgcg gctggccacc ggcgacgccg
     3541 agaccgcgct cgaggcgttc cgtcgcgccc agggcttcac gccgccgccg ggccggccgc
     3601 tggaggtcaa gttctgggag gccgaggcgc tgttccggct caagcgcttc gccgaggcgc
     3661 gcgcggcgta cgacgcggtc gtgcggaccg acgccgcctc gccgctggcg cccgacgccg
     3721 tctacgggct ggcgtggagc gagagcgagc tgggccacgc cgagaccgcg gtcgacgcgt
     3781 tccggcagtt cctgcagacg tggcccgacc acgcgacggc gccgtcggcg accttccacc
     3841 tggcgcgcgt tctcgtggag ctcaagcgct tcaaggaggc gctgccgctg ctcgaggagt
     3901 tcaccggccg ctaccgcacc cacgcgcagg cgccggacgc gcgctacctg ctcggcctgg
     3961 cgcgcatcga gacgggcgac acgcgcggcg gcgtcgccga cctgcgggcg ttcgccgagg
     4021 cacaccccac gcatccgcag acgcgtgccg cgcggcagct cgtcacgcgg acgctggcgc
     4081 gccacggcaa ccgcagcgag ctgggcgagg tctacaagac gctgatggcg gagtccccgg
     4141 cgacgcccga cggcctctac gatgccgcct cggtggccgg ccggctcggg cgcgccaagg
     4201 agcaggaggc cgccctgcgg cggctccgcg gggagttccc cgaccacgcg ctctcgcggc
     4261 gcgcggcgct cgagctggcg tccgtcgcct acaagcgcaa ggactggaag gacacggtgg
     4321 cgcaggcgga ggccgcgacg aagagcggcg agagcgccgt gcgcgcggag gcatggctgc
     4381 tcgtcggcga gggcgagctg aagctcaagc gcttccagaa cgccgaaaag gcgttcgagg
     4441 ccgcgggggc cgtgaaggag gcgaagcccg ccattcgctt ccgcgcgctg gccggtctcg
     4501 gcctggcgca cgaggagcag cgcgagtgga agccggcgct gacggcctat gaagccgttg
     4561 ccgacaagag ccccgatgcg accctgcgcg actgggccag ggagcgcgcg acggcggtca
     4621 aggagcgcat ggcctcggcg cccccggaca cgaagccccc cgagcagaag gcccccgaga
     4681 agacgccggc cgtgaagacg ccgcccgcga agacgccggc cgagaagaag cccacggaga
     4741 ggaaggcggg ctcgtgatgc gtcgggtcgt cgtcctcgcc ctggtgtggg gcgcctcggt
     4801 ggcggcaacg cccatcgccc ccacgctgcc gccgccggac ctggcgccgc tcgtgccctg
     4861 ggccgtcgcg cccctcgaca agcccgcggt ggagcttccg aagctggcgc ttccaccctc
     4921 gccggccgac gttccgaccg tcgcgccggc gacggtcgcg ctgccggagg cgcagaaacc
     4981 gaccgagtcg ctgccgtctg cgcgaacgct cccgtgcgtg ggcgcgtggc ttcgcatcgc
     5041 ctccgagtcg ctcgaatgcg gccgcgcgaa gctcggccgg ggcgagctgg acgaagccgc
     5101 ccgcgccctc gagcacgccg cacgccccgg tcccgagccg gatgtcatcg ccgaggctcg
     5161 ctactggcac gccgagacgc tcttcaggct cgggcagttc gcacgcgccg actggctctt
     5221 ccgccaggtc gtcaccgacc cggcccgcac cggcctcagg ccgtgggcga tcttcggtag
     5281 cggcttcacc gcgcttcggc tcggtgacgc cccgcgcgcg caggaggcct tccggcgcgt
     5341 gctgggcgtc gcccaccccg ctgctctcga cgggtgggcg cgccacggcc tggccctggc
     5401 cctctacgcg cagagccgct ggcaggacgc cgacaagacg tggaccgacg cgacgtcgcg
     5461 ccggctgccg ccggctctgg agcgcgacgt cctcttctgg cacggcgaca cgctcggccg
     5521 ggtccgtcag ccaccgcgcg ccgtcgagaa gctggcgagc ttcgcccagg gtgggccgca
     5581 cccgctgctc acgcccggtc tcgtgcgact cggctggtgg tcgctcgctg ccggggcgcc
     5641 ggccgaggcc ctcaaggcgc tgcggacggc gctcgtcagc gcgccgaccg ccggtcccga
     5701 ggttcgcgag cgcgagtggg ccgaggccgg cctcgccctc gcgctgggcg ccacgggcga
     5761 cctgccgggg gcccgcgcgt cgctggcgcc actcgacggc cgtcgctcgg cgctcgcgct
     5821 ccccgtccgg ctccgcctga tcgacgcggc ggtggagagc ggccagccgg cggaggccct
     5881 ggcgctggcg caggatctgc tcagcggaaa cctgacgccg ccggtgcgcg cgtgggtgct
     5941 gctcatgaag ggcgaggcgc accgcaccga aggcaatcgc gacgaggcac gcacccagtt
     6001 cgacctcgct cgcgatggaa ctccccgggg cgacgtgggg cgccacgccg cgctgcggat
     6061 cgcccagacg aacttcgagc tgcgcgagtt ctcgcaggtc gtcgccgaca cgggcccgtt
     6121 gctcggggcg gccgtcgctc cggagatgcg agccgcggcc ctgctggtgc agggcgaggc
     6181 cgcataccac gcgggcaact actccgtcgc cgcggactcc taccgtcgtc tgctcgtcga
     6241 gttcccggac cacgagcagg cgacggccgt gcgcctggcc ctggcctgga cggtgctgcg
     6301 ccagggccag cgcgacgccg cgcgccagca gttcctcgag ttcgcgaggg ctcatcccac
     6361 gaacgcccac gcggtggacg ccctggtgct ggcctccgag ctgcacctgg ccggcggcga
     6421 ccttcc
//
LOCUS       DATIAZ010000212         6500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1767846, whole genome shotgun sequence.
ACCESSION   DATIAZ010000212 DATIAZ010000000
VERSION     DATIAZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767846"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VLF19_07595"
     CDS             <1..594
                     /locus_tag="VLF19_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93154.1"
                     /translation="DQISPDKLRELLEEMERLGRKGDGNWASDISEGMEALDYGQQDK
                     AMEAMEKALNKLRAMEESQRAGKGLRGGREGQRGRDRDRERGGGGGLPEEGDFGEGEG
                     LLPGKGKSTSPKGEATQRLRASPYDVGVEGESGRGRKDGYDTNMTGRGGKMGSRLTYL
                     GVIGQYRKAMEDTLAREQVPRDYHGQIKDYFQSLDER"
     gene            599..1489
                     /locus_tag="VLF19_07600"
     CDS             599..1489
                     /locus_tag="VLF19_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSE93155.1"
                     /translation="MTTAAPPAVFTSDFLAQLERLSLASRRAFRGRVKGERRSPRKGQ
                     SVEFSDYRPYGIGDDLRYVDWNIYGRLDRLHVKLFVDEEDLCLHLIVDASASMGFGRP
                     TKLAYATRLAAAVGFVGLVNLERVGVGVLREKVSEGWPPVRGRNQFVKMVDFLSAVRS
                     GGATSLNEGLANYAMRSREPGVAVIVSDLLDPAGFETGIRALLERRFEVHVVHLLAAE
                     ELNPAMTGDLRLHDSETGEVRELSVDAEALRAYRQRLQHFLERAEAFCRGQEVGYRRA
                     STDVPVEEFVLAQLRGLVLV"
     gene            1497..5876
                     /locus_tag="VLF19_07605"
     CDS             1497..5876
                     /locus_tag="VLF19_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSE93156.1"
                     /translation="MSFLAPLALAAFALSLPLVLLYFLKVRRREQVVPSLLLWEPTLR
                     DREASTFFQRLQRDPLLILQILALAALTLALARPAMTVMGEGARKVVVVLDASASMKA
                     REGGTSRFAMARAQASALVGRLGEGAEVMIIESGMQPRVLAPLSRDRERAGAAIRAAQ
                     ARDLPHRLPEAIRTARALVGADPRAEIHVFTDGAFALGDNPDALDPRLRWVTVGQGSR
                     NVGITNLAVRKAYYGSFDYQAFASLVNTSARTQTFAFTLEVDGTTLAERQLSLEPSVR
                     RSLVLPFSHTGGGTVKAIIDARDDFAVDNVAYAVLPPPRKIQVLLVSPGNLFLEKVLR
                     TDPQVAVEVQTPEQYQGGMAQADIVVLDSVTPARVGAGRFVFVNTVPGDVPLEVLGRI
                     ERPTIMDWDRAHPVMRHVEFAKVVIEDALRIRPLAAGRPLVEAVGGPVIYALEEPERK
                     AVFIGFDLFKTDFPLRVAFPLILSNSLRWLHPAGLDQASLQLAAGQPILLPVEHGVSS
                     VTVTTPSGRSVKAQVTRGVVSFTETDEVGVYTLATPRGQLRVAVNLADAEESNLAPRP
                     LPSPPAAGAEGSTTLPVQRELWQLFVLVAAILLIVEAALYWRRQTGGLSRAPASSRDR
                     WALALRGALVLVLCATFLKPTLPRMVDSQNVAFLLDLSDSVSLAARERAWRFAAEAVK
                     SARAADRSSLIVFGDDSVVDQALASRPTLDRPKAEVTARGTNLFQAIQLALATLPPGH
                     ANRIVLLTDGRQTAGSALAGAQAAKNAGADIYYVAAPLTFDQEVVAESMVLPQEVKYG
                     EPFQARVVAWSLKDTQGRLSLFRNGEFLGSQVVRLTAGKNVFSYRQSLDASGIHVYQA
                     ALEVDGDTIEDNNRAVGTVVVRGRPQVLLAEKDRSHAQSLAGALRSQNIEVTVVAADQ
                     IPKDVAGLQKYDGVILSNVSSLKLTRAQMSNIRDYVRDHGGGLIMVGGEESFGLGGYY
                     RTPIEEALPVTMEVKQKVEIPSLAVVLSVDRSGSMAMSTGDEKVTKLDLAKEAAHLVV
                     DLLDERNEVGVMSWDTEFIWDVSVRMARDKAAIHHSVATIKAGGGTDGYPALKESYAV
                     LFERPALLKHVIFLSDGQMTRGDFQGLLRRMVKDKITVSSVAIGKDADLQLMVDIAKW
                     GRGRFYYTEDSQTIPRIFTLETQLASKASLIEQPFRPQLTAPGHEAMQDVDWKAVPPL
                     GGYVAATVKSTAELVLMSHQEDPVLATWRYGLGRAAAFTSDAKAKWATLWLRWRDFNK
                     FWAQLTRWTLRSSTRSDTVALVERRDGMGEVLVDAVDGKGEFINFLDSQVGVVAPNRE
                     RTVIDLEQVAPGRYRGRFAAPHEGVYLVGMAQRVQDRVVGSQLAGLVVPYAQELRDLG
                     VDEAGLRELAELTGGGPLVQPREAFLKSRRQSRLAVEIWPWLVGLVAVLMVPEIALRR
                     LGPGALRWLGALGRRRREGGEHA"
     gene            5869..>6500
                     /locus_tag="VLF19_07610"
     CDS             5869..>6500
                     /locus_tag="VLF19_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93157.1"
                     /translation="MPDLLRTLLPITALVVLALLVVAAPGDAAVKPRVLLVVPYDAAS
                     LGGDGQWVGEGIAEIVRLGLAQHPAFVQLDTARLRQFGQPEIWGDDAVQQAARALRAD
                     AALFGRVVKSGTDYTIQPMLYDIKASGGDMLPLEPMTIPEADLMQRLPAVVVAYVRSM
                     RIAPTDAELGRIEKAARPTRSLRAFELYARSQQALRRGGQEGNESAADLLS"
BASE COUNT          930 a         2258 c         2348 g          964 t
ORIGIN      
        1 gaccagatct ctcccgacaa gctccgcgag ttgctcgagg agatggagcg cctggggcgc
       61 aagggcgacg gcaactgggc ctcggacatc tccgagggca tggaggcact cgattacggg
      121 cagcaggaca aggccatgga ggccatggag aaggcgctga acaagctgcg cgccatggag
      181 gagtcgcagc gcgccggcaa gggcctgcgt ggcggtcgcg agggccagcg cggccgtgac
      241 cgcgaccggg agcggggcgg cggcggcgga cttccagagg agggcgattt cggcgagggg
      301 gagggcctcc tgcccggcaa gggcaagagc acgtctccga agggcgaggc cacccagcgc
      361 ctgcgcgcca gcccctacga cgtcggcgtc gagggcgagt ccgggcgcgg ccgcaaggac
      421 gggtacgaca cgaacatgac cgggcgcggg gggaagatgg ggtcgcggct cacctatctc
      481 ggcgtgatcg gccagtaccg caaggcgatg gaggataccc tggcccgcga gcaggtgcct
      541 cgagactacc acggccagat caaggactac ttccagtccc tggacgagcg ctgagtcggt
      601 gacgacggcg gccccgccgg cggtcttcac gtcggacttc ctggcccagc tcgaacgcct
      661 gtcgctcgcc tcgcgccgcg cgttccgcgg tcgggtgaaa ggcgagcggc ggagcccgcg
      721 caaggggcag agcgtcgagt tctcggacta ccgtccgtac ggtatcggcg acgacctgcg
      781 ctacgtggac tggaacatct acggccggct cgaccgcctg cacgtcaagc tcttcgtcga
      841 cgaggaggac ctgtgcctgc acctcatcgt cgacgcctcg gcgtcgatgg gcttcggccg
      901 cccgaccaag ctggcctacg cgacgcggct ggccgccgcc gtcggcttcg tcggcctggt
      961 caatctcgag cgggtcggcg tgggcgtgct ccgcgagaag gtcagcgagg gctggccccc
     1021 ggtgcgcggg cggaaccagt tcgtgaagat ggtggacttc ctctccgccg tccgctccgg
     1081 cggcgcgacc agcctcaacg aggggctcgc gaactacgcc atgcgctcgc gcgagccggg
     1141 tgtggccgtg atcgtctcgg acctgctcga cccggcgggc ttcgagacgg gcatccgcgc
     1201 gctgctcgag cggcgcttcg aggtccacgt cgtccacctg ctcgccgccg aggagctgaa
     1261 ccccgcgatg accggcgacc tgcgcctcca cgacagcgag accggagagg tgcgggagct
     1321 ctcggtggac gccgaggcgt tgcgcgctta ccggcagcgc ctgcagcact tcctggagcg
     1381 cgccgaagcg ttctgccgtg ggcaggaggt cggctatcgc cgcgcgagca cggacgtccc
     1441 cgtggaggag ttcgtgctcg cgcagctgcg cggcctggtg ctcgtgtgag ggccgcgtga
     1501 gcttcctggc gccgctggcg ctcgccgcct tcgcgctgtc gctgccgctc gtgctgctgt
     1561 acttcctcaa ggtgcggcgc cgggagcagg tggtgccgag cctcttgctg tgggagccca
     1621 cgctgcgcga ccgcgaggct tccacgttct tccagcgcct gcagcgcgac ccgctcctga
     1681 tcctgcagat cctcgcgctg gcggcgctga cgctggcgct cgctcgcccg gcgatgaccg
     1741 tgatgggcga gggcgcgcgc aaggtcgtcg tggtgctgga cgcttcggcg tcgatgaagg
     1801 cgcgcgaggg cgggacgtcg cgcttcgcga tggcgcgcgc ccaggcgtcg gcgctggtgg
     1861 gacgcctggg tgagggcgcc gaggtgatga tcatcgagtc ggggatgcag ccgcgggtgc
     1921 tggccccgct ctcgcgcgat cgcgagcgcg caggcgccgc catccgcgcc gcccaggcgc
     1981 gggacctgcc tcaccggctg cccgaggcca tccgcacggc gcgcgcgctc gtcggcgccg
     2041 acccgcgcgc cgagatccac gtgttcaccg acggggcctt cgcgctcggc gacaatcccg
     2101 acgcgctcga tcctcggctc cgctgggtca cggtgggcca ggggagccgc aacgtcggca
     2161 tcaccaacct cgccgtgcgc aaggcctact acggctcgtt cgactaccag gccttcgcct
     2221 cgctcgtcaa cacgagcgcc cggacgcaga cgttcgcctt cacgctcgag gtcgacggca
     2281 ccacactggc cgagcgccag ctctcgctcg aaccgagcgt gcggcgctcg ctcgtcctgc
     2341 cgttcagtca caccggcggg ggcaccgtga aggccatcat cgacgcgcgt gacgacttcg
     2401 cggtcgacaa cgtcgcgtac gcggtgctgc cgccgccgcg gaagatccag gtcctgctcg
     2461 tcagtcccgg caacctcttc ctcgagaagg tgctgaggac cgacccgcag gtagcggtgg
     2521 aggtgcagac gcccgagcaa taccaggggg gcatggcgca agccgacatc gtcgtgctgg
     2581 actcggtgac gccggcgcgg gtgggtgccg ggcgcttcgt gttcgtcaac acggttcccg
     2641 gcgacgtgcc gctggaggtg ctcggccgca tcgagcggcc cacgatcatg gactgggacc
     2701 gcgcgcatcc cgtgatgcgc cacgtcgagt tcgccaaggt cgtgatcgag gacgcgctca
     2761 ggatccggcc gctggccgcg ggccggccgc tcgtggaggc cgtcggcggc ccggtcatct
     2821 acgccctgga ggagccggag cgcaaggccg tcttcatcgg cttcgacctc ttcaagaccg
     2881 acttcccgct ccgcgtggcg ttcccgttga tcctctcgaa cagcctgcgc tggctgcacc
     2941 cggccgggct cgaccaggcg agcctgcagc tggcggcggg gcagccgatc ctgctccccg
     3001 tcgagcacgg cgtcagctcg gtcaccgtga ccacgccgag cggccgctcg gtgaaggcgc
     3061 aggtcacgcg cggcgtggtg agcttcaccg agaccgacga ggtcggcgtg tatacgctcg
     3121 cgaccccgcg cgggcagctg cgcgtcgccg tcaacctggc cgacgccgag gagtcgaacc
     3181 tggccccacg gccgctgcct tcgccgccgg cggcaggcgc cgaggggagc acgacgctgc
     3241 ccgtgcagcg cgagctctgg cagctcttcg tcctcgtcgc cgcgatactc ttgatcgtcg
     3301 aggccgcgct ctactggcgg cgccagacgg gcggtctgtc gcgcgcgccg gcgagctccc
     3361 gcgaccgctg ggccctggcg ctgcgcggcg cactcgtcct ggtgctgtgc gcgacgttcc
     3421 tcaagcccac gttgccgcgg atggtggaca gccagaacgt cgcgttcctg ctggacctgt
     3481 ccgacagcgt gagcctggcc gcgcgcgagc gggcctggcg cttcgcggcc gaggccgtga
     3541 agtcggcgcg cgccgccgac cgctccagcc tgatcgtctt cggcgacgac tccgtggtcg
     3601 accaggcgct cgcctcccgg ccgacgctcg accgacccaa ggccgaggtg acagcgcgcg
     3661 gcaccaatct cttccaggcc atccagctcg cgctggcgac gttgcccccg ggccacgcga
     3721 atcgcatcgt gctgctcacc gacggacggc agacggccgg cagcgcgctg gcgggcgcgc
     3781 aggcggcgaa gaatgccggt gccgacatct actacgtggc ggcgccgctg acgttcgatc
     3841 aggaagtcgt cgcggagtcg atggtgctgc cgcaggaggt gaagtacggc gagccgttcc
     3901 aggcccgggt cgtggcgtgg agcctcaagg acacccaggg ccggctctcg ctcttccgca
     3961 acggcgagtt cctcgggtca caggtcgtgc gcctgaccgc gggcaagaac gtcttcagct
     4021 accgccagtc cctcgacgcc agcggcatcc acgtctacca ggcggccctc gaggtcgacg
     4081 gcgacacgat cgaggacaac aaccgcgcgg tgggcacggt cgtggtacgc gggcgacctc
     4141 aggtgttgct ggccgagaag gaccgcagcc acgcccagtc gctggccggc gcgctgcgct
     4201 cgcagaacat cgaggtcacc gtcgtcgccg cggaccagat cccgaaggac gtcgcgggcc
     4261 tgcagaagta cgacggggtg atcctgtcca acgtctcctc gctgaagctc acgcgggctc
     4321 agatgagcaa tattcgcgac tacgtccgcg accacggcgg cggcctcatc atggtgggcg
     4381 gcgaggagag cttcggtctc ggcggctact accgcacgcc gatcgaggag gctttgccgg
     4441 tcacgatgga ggtcaagcag aaggtcgaga tcccgagcct ggccgtggtg ctctccgtcg
     4501 accggtcggg ctccatggcc atgtcgacgg gagacgagaa ggtcaccaag ctcgacctgg
     4561 ccaaggaggc cgcccacctc gtcgtcgacc tgctcgacga gcgcaacgag gtcggggtga
     4621 tgagttggga caccgagttc atctgggacg tgtcggtgcg catggccagg gacaaggcgg
     4681 ccatccacca ctcggtcgcg accatcaagg ccggcggcgg caccgacggc taccccgccc
     4741 tcaaggagtc ctacgcggtg ctgttcgagc gcccggcgct cctcaagcac gtcatcttcc
     4801 tctccgacgg ccagatgacg cgcggtgact tccaggggct gctccgccgg atggtgaagg
     4861 acaagatcac ggtctcgtcg gtggccatcg gcaaggacgc cgacctgcag ctcatggtgg
     4921 acatcgccaa gtggggacgc ggccgcttct attacaccga ggactcccag accattcccc
     4981 ggatcttcac gctggagacc cagctcgcct ccaaggcttc gctcatcgag cagccgttcc
     5041 ggccgcagct cacggctccc ggccacgagg cgatgcagga cgtggactgg aaggccgtgc
     5101 cgcccctcgg cggctacgtc gccgcgaccg tcaagagcac ggcggagctg gtcctgatga
     5161 gccaccagga agacccggtc ctcgccacgt ggcgttacgg cctcggtcgg gctgccgcgt
     5221 tcacctcgga cgccaaggcg aagtgggcga cgctctggct gcggtggcgc gatttcaaca
     5281 agttctgggc gcagctcacc cgctggacgc tccgcagctc gacgcgcagc gacacggtgg
     5341 ccctcgtcga gcggcgcgac ggcatgggcg aggtgctcgt ggacgctgtc gacggcaagg
     5401 gggagttcat caacttcctc gactctcagg tcggcgtggt ggcccccaac cgtgagcgca
     5461 cggtcatcga cctcgagcag gtcgcgccgg ggcgctaccg tggccgcttc gcggcgcccc
     5521 acgagggagt ctacctcgtc ggcatggccc agcgcgtcca ggaccgcgtg gtcggctccc
     5581 agctggccgg cttggtggta ccgtatgctc aggagttgcg cgacctcggg gtggacgagg
     5641 caggcctgcg cgagctggcc gaactgaccg gcggcgggcc gctcgtccag ccacgcgagg
     5701 ccttcttgaa atcgcggcgg cagtcgcggc tggccgtgga gatctggccc tggctggtcg
     5761 gcctggtggc cgtgctgatg gttccggaga tcgcgctgcg gcgtctcggg ccgggcgcgc
     5821 tgcggtggct cggcgcgctg ggacggagac ggcgggaggg aggagagcat gcctgatctt
     5881 ctgcggaccc tgcttccgat caccgccctg gtcgtgctgg ccctcctcgt ggtcgcggcg
     5941 ccgggcgatg ctgcggtgaa gccacgagtg ctgctggtgg tcccctatga tgccgcttcg
     6001 ctcggcggcg acggacagtg ggtgggcgag gggatcgcgg agatcgtgcg gctcgggctc
     6061 gcccagcatc ccgccttcgt gcagctggac accgcgcggc tgcgtcagtt cgggcagccg
     6121 gagatctggg gcgacgacgc cgtgcagcag gcggcccgtg cgctgcgcgc cgacgccgcg
     6181 ctgttcggtc gtgtcgtcaa gagcggcacg gactacacca tccagccgat gctgtacgac
     6241 atcaaggcgt ccggcggcga catgctaccg ctcgagccga tgaccatacc cgaggccgac
     6301 ctcatgcagc gtctgccggc tgtcgtcgtc gcctacgtgc ggtcgatgcg gatcgccccc
     6361 acggatgccg agctcgggcg catcgagaag gcggcgcggc ccactcgctc gctgcgcgcc
     6421 ttcgagctct acgcgcgcag ccagcaggcc ctgcgccgcg gcggtcagga gggcaacgag
     6481 agcgcggcgg acctcctctc
//
LOCUS       DATIAZ010000213         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1636627, whole genome shotgun sequence.
ACCESSION   DATIAZ010000213 DATIAZ010000000
VERSION     DATIAZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636627"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VLF19_07615"
     CDS             <1..1277
                     /locus_tag="VLF19_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE93158.1"
                     /translation="MPGLDGLTVLERVRELRPDTYVVVMTAHGTMETAITAMQRGAYD
                     YVAKPFDLDEVLLLAERALAAGRLTQEVARLRRGLQEVREFSALIGRHPRMQDVYKTI
                     GRIAGTDVTVLLRGESGTGKELVARAIHHYSRRSGRPFVAVSCAAIPATLLESEMFGH
                     ERGAFTDAKERRLGKFELAHGGTLYLDEIGDMPLELQAKVLRALQERTIERLGGQEPI
                     RIDVRVLAATNRDLESLMREDRFREDLYYRLNVVTLNLPPLRERRRDVPLLVEHFLGK
                     HADELGERGVAPEALDRLVGHDWPGNVRELENVVQRAMVLATNGVILPEHLPIGPVSA
                     AASVAVDATLEDIIERKLMECVRGLRDRSAANLYDLMVGLVEKPLLRAVLRETMGNQL
                     RAAQILGINRNTLRKKLTEHGIDPDAFVDASA"
     gene            1513..1935
                     /locus_tag="VLF19_07620"
     CDS             1513..1935
                     /locus_tag="VLF19_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93159.1"
                     /translation="MHSTFEDRSPRVLVVDDEGHVRSMLCDLLAMWGCQVDAAPSAAQ
                     GLDLLERGIYDLVLTDFRMPDMTGIDLVERIRARHGRLCVVMLTASAADFDAMGRRLG
                     FTLLRKPLEIDDLKAALHQALDRSAGNAPAAAAAHDGS"
     gene            complement(1938..2186)
                     /locus_tag="VLF19_07625"
     CDS             complement(1938..2186)
                     /locus_tag="VLF19_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSE93160.1"
                     /translation="MGRLTCRDVIDLLVDYLESTLTDEICTALEQHLQDCAPCVAYLN
                     TYRKTRDLAGAEGRVAMPEEMRQRLHAILLARLTERRD"
     gene            complement(2218..>3072)
                     /locus_tag="VLF19_07630"
     CDS             complement(2218..>3072)
                     /locus_tag="VLF19_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE93161.1"
                     /translation="FEAASDAEVTGEAYERLSGVFGLRQRVVRLRLGGETIELTEFLA
                     PRGRAIPPDLRSHDRTFQHIAIVVRDIDEAYRHLRAHRVTHVSPGPQRLPDWNPAAAG
                     IAAFYFKDPDGHVLEIIQFPPDKGGPQWRQATARLFLGIDHTAIVVADTDAALRFYRD
                     LLGLVVTGTSENHGPEQERLNAVFGARLRITTLRAATGPGIELLEYLTPRDGRPLPAD
                     ARVNDIAHWQTTLVTRDPALLAPRLRAARAVFVSSGVVTLEDRKLGFERAVLVRDPDG
                     HALQVVAP"
BASE COUNT          451 a         1066 c         1107 g          448 t
ORIGIN      
        1 ggatgccggg cctcgacggg ctcaccgtgc tcgagcgcgt gcgcgagctg cggccggaca
       61 cctacgtcgt cgtgatgacc gcgcacggca cgatggagac ggccatcacg gccatgcagc
      121 gcggcgccta cgactacgtc gcgaagccct tcgacctcga cgaggtgctg ctgctcgccg
      181 agcgggccct ggccgccgga cggctcacgc aggaggtggc gcggctgcgc cgcgggctgc
      241 aggaggtgcg cgagttcagc gcgctcatcg gacgccaccc ccggatgcag gacgtctaca
      301 agacgatcgg ccgcatcgcc gggaccgacg tcaccgtgct gctgcgcggc gagtcgggca
      361 ccggcaagga gctggtggcg cgcgccattc accactacag ccggcgctcg ggacgtccgt
      421 tcgtcgccgt gtcgtgcgcg gccatcccgg cgacgctgct ggagtcggag atgttcggcc
      481 acgagcgcgg cgcgttcacg gacgccaagg agcggcgcct gggcaagttc gagctggctc
      541 acggcggcac gctctacctg gacgagatcg gcgacatgcc gctcgagctg caggccaagg
      601 tcctgcgcgc gctccaggaa cgcacgatcg agcggctcgg tggccaggag ccgatccgca
      661 tcgacgtgcg cgtgctggcc gcgaccaacc gggacctcga gagcctgatg cgggaggacc
      721 gcttccggga ggacctctac taccgcctga acgtcgtgac cctcaacctg ccgccgctcc
      781 gcgagcgccg ccgcgacgtc ccgctgctcg tcgagcactt cctcggcaag cacgccgacg
      841 agctgggaga gcgcggcgtg gcgccggagg cgctggaccg tctggtgggc cacgactggc
      901 cgggtaatgt gcgcgagctc gagaacgtcg tccagcgagc gatggtgctg gcgaccaacg
      961 gcgtgatcct gcccgagcac ctgccgatcg gcccggtctc ggcggcggcc tcggtcgcgg
     1021 tggacgcgac gctcgaggac atcatcgagc ggaagctgat ggagtgcgtg cgcggcctgc
     1081 gcgatcgttc ggcggcgaat ctctacgatc tgatggtcgg cctcgtcgag aagccgcttc
     1141 tgcgggccgt gctgcgcgag acgatgggca accagctgcg ggccgcccag attctaggca
     1201 tcaatcgcaa cacgcttcgc aagaaactga ccgagcacgg aatcgatccc gacgccttcg
     1261 tggacgccag cgcctgagcg tcgggttttc cacttttcca cacgcatcca catctcgaaa
     1321 ttcgaccgga gtgatgtgca tcgacggacg gttggcgcct gatcgaccgt cgccgcgcgg
     1381 tcacccggag cggagggacc tcctcgccgc tcaagacgcg ggccagcgcg cgtcggcgac
     1441 cgagaaagcg cgtgcctagc ggccgccaat cccattgatc gacacgcggg gctccggtag
     1501 gctctggcga tcatgcactc gaccttcgaa gaccgctcgc cccgcgtgct cgtcgtcgac
     1561 gacgaagggc acgtccgctc gatgctctgc gacctgctcg cgatgtgggg ctgccaggtg
     1621 gacgccgcgc ccagcgcggc gcagggcctc gatctcctcg agcgcggcat ctacgacctc
     1681 gtgctcacgg acttccgcat gcccgacatg acgggcatcg atctggtgga gcgtatccgc
     1741 gcccgccacg ggcggctctg cgtggtcatg ctgacggcat cggccgcgga cttcgacgcg
     1801 atgggccgcc ggctcggctt caccctgttg cgcaagccgc tcgagatcga cgacttgaaa
     1861 gccgcgctgc atcaggcgct cgaccggtcg gccgggaacg cgccggccgc ggcggccgcg
     1921 cacgacggct cgtagggtca gtcccgccgc tcggtgagcc gcgccagcag gatggcgtgg
     1981 agccgctggc gcatctcctc gggcatcgcg acacgcccct cggcgccggc gaggtcccgg
     2041 gtctttcgat aggtgttgag gtaggccacg cacggtgcgc agtcctggag atgctgttcc
     2101 agcgcggtgc agatctcgtc cgtgagcgta gattcgaggt agtccaccag gaggtcgatc
     2161 acgtcccggc aggtcagcct gcccacggcg ccgccaccct tcatcgccca tcgcccttca
     2221 cggcgcgacg acctgcagcg cgtgaccgtc ggggtcgcgc accagcaccg cgcgctcgaa
     2281 gccgagcttc cggtcctcca gcgtgacgac gcctgacgag acgaagacgg cgcgcgcggc
     2341 gcgcaggcgt ggggccagca gcgccgggtc cctggtgacg agggtggtct gccagtgcgc
     2401 gatgtcgttg acgcgggcat ccgccgggag gggccgaccg tcgcgcggcg tcaggtactc
     2461 cagcagctcg atgccggggc ccgtcgccgc gcgcagggtg gtgatccgca ggcgcgcccc
     2521 gaagacggcg ttcagccgct cctgctcggg gccgtggttc tcgctcgtgc cggtgacgac
     2581 gaggcccagc agatcgcggt agaaccggag ggcggcgtcg gtgtcggcca cgacgatcgc
     2641 ggtgtggtcg atgccgagga agagccgcgc ggtcgcctgg cgccactgcg ggccgccctt
     2701 gtccggcggg aactggatga tctcgaggac gtgcccgtcc gggtccttga agtagaaggc
     2761 ggcgatgccg gctgcggccg ggttccagtc gggcagtcgc tgaggacccg gcgagacgtg
     2821 ggtcacccgg tgcgctcgca ggtggcggta cgcctcgtcg atgtcacgga cgacgatggc
     2881 gatgtgctgg aacgttcggt cgtgactgcg caggtccggc gggatcgcgc gcccgcgcgg
     2941 cgccaggaac tccgtcagct cgatcgtctc cccgcccagg cgcagccgta ccacgcgctg
     3001 gcggagaccg aagacgcccg agaggcgctc gtacgcctcg ccggtcactt ccgcgtccga
     3061 cgcagcctcg aa
//
LOCUS       DATIAZ010000214         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_453394, whole genome shotgun sequence.
ACCESSION   DATIAZ010000214 DATIAZ010000000
VERSION     DATIAZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453394"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1360
                     /locus_tag="VLF19_07635"
     CDS             368..1360
                     /locus_tag="VLF19_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HSE93162.1"
                     /translation="MNIAVLDDYQDTIRTLACFEKVSGHSVTIWNDHTKDVDELAGRL
                     KDTEVLALIRERTPIREPLLERLDKLRLITQVGVVPHIDLEACTRRGVIVSSSTMPGR
                     PSYATAELNWGLIIAALRRIPQEMAALKAGRWQAYPIGTGLRGKTLGIFGYGKIGGVV
                     AGFGKAFGMNVLVWGRQTTTDRARADGYRIAAGKEALFEQSDVLSLHLRLIAETRGIV
                     TAADLARMKPTALIVNTSRAGLIAPGALEAALRAGRPGMAAVDVYEDEPVLGGRHPLL
                     SMDNVVCAPHLGYVERDGLEHMFSTIFDEILAYAEGKPINVVNPEVLEKGTARR"
     gene            1357..2526
                     /locus_tag="VLF19_07640"
     CDS             1357..2526
                     /locus_tag="VLF19_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HSE93163.1"
                     /translation="MTIKGKAYIAGVFEHPTRKADDKTVAQLHAEVARGALEDAGLTK
                     NDIDGYFCAGDAPGLGPLSMADYMGLTPRHVDTTDTGGSSYVIHVAHAAQAIALGKCK
                     VALITLAGRPRSEGMATGTAPRVPAAAMPDAPFEFPYAPATANMYAMAAMRHMHQFGT
                     TSAQLAWVKVAASHHAQHNPHAMLREVVTVEDVVNSPMITDPLHRLDCCVISDGGGAV
                     VVVAPEIARSLRRPRVRVLGAGEALKLQMGGKVDLTYTGAVWSGPAAFAEAGVKPADI
                     KYASIYDSFTITVLMTLEDLGFCEKGQGGKFVADGNLVSGVGRLPFNTDGGGLCNNHP
                     ANRGGMTKVIEAVRQLRGEAHPKVQVKNCDVALAHGTGGSLGTRHASATLIMERE"
     gene            2532..>2887
                     /locus_tag="VLF19_07645"
     CDS             2532..>2887
                     /locus_tag="VLF19_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HSE93164.1"
                     /translation="MAPAQRKIPAPQVTPETKPFWDAAAEGRLVIKQCTACDQPHHYP
                     RTICPLCGSDATEWRPASGRGTIYTYSVMRRVPEPYAIAYVTLEEGPTMMTNIVDCDL
                     DAIRIGQAVRVVFAPS"
BASE COUNT          463 a         1039 c          995 g          390 t
ORIGIN      
        1 cgcgccttca gggacccgct ccggcccgtg ttcggcacca gcctcgaaga cttccagcgc
       61 cgggcgcgcg cggtgacctc ggggccgatc tacctgcccg ggccgcgctc gaagctctac
      121 atccccatcg accggcggtg acactcacgc ggcggccgat gccacctcca gcgaccccgg
      181 gatggagatg ggcgccagcg ccctcgaggc gatcctcctc gaggtgcggc gttccggtct
      241 cgaccgacct ccttccgtga ctcccgctcg gtcgccgtct cccggtctcc ggatcccgcc
      301 cgatttgact ccgtggtagg ctgcccgggc gcgccgggat ctgctcgcgg cgccacgagg
      361 ggacaggatg aacatcgccg ttctcgacga ctatcaggac acgatccgca cgctggcctg
      421 cttcgagaaa gtctccggcc acagcgtgac catctggaac gaccacacca aggacgtcga
      481 cgagctggcc gggcgcttga aggacacgga ggtgctggcg ctcatccgtg agcgcacccc
      541 gatccgagag ccgctgctcg agcgcctcga caagctgcgc ctcatcaccc aggtcggcgt
      601 cgtcccccac atcgacctcg aggcctgcac gcggcgcggc gtgatcgtgt cgtccagcac
      661 gatgccgggc cggccctcct acgccaccgc ggagctcaac tgggggctca tcatcgccgc
      721 gctccggcgc atcccgcagg agatggccgc gctcaaggca ggccgctggc aggcgtaccc
      781 catcggcacc ggcctgcgcg gcaagacgct cgggatcttc gggtacggca agatcggcgg
      841 cgtcgtggcc ggcttcggca aggccttcgg catgaacgtg ctggtctggg ggcgtcagac
      901 gacgaccgac agggcgcggg ccgacggcta tcgaatcgcc gccggcaagg aggcgctgtt
      961 cgagcagtcc gacgtcctgt cgctgcacct ccggttgatc gccgagacgc gcggcatcgt
     1021 caccgccgcc gatctcgccc ggatgaagcc caccgcactc atcgtgaaca ccagccgggc
     1081 tgggctgatc gcgcctggcg cgctggaggc cgcgctacgc gccggccggc cggggatggc
     1141 cgcggtcgac gtgtacgagg acgagccggt gctcggcggg cgccacccgc tgctgtcgat
     1201 ggacaacgtc gtctgcgcgc cgcacctcgg ctacgtggag cgcgacgggc tcgagcacat
     1261 gttcagcacg atcttcgacg agatcctcgc ctacgcggag ggcaagccga tcaacgtcgt
     1321 gaaccctgaa gtgctggaga aggggacggc acgccgatga ccatcaaggg caaggcctac
     1381 atcgcgggcg tcttcgagca ccccacgcgc aaggccgacg acaagacggt ggcccagctg
     1441 cacgcggagg tcgccagggg cgcgctcgag gacgcggggc tcaccaagaa cgacatcgac
     1501 ggctacttct gcgcgggcga cgcgcccggg ctcgggccgc tgtcgatggc cgactacatg
     1561 ggactcacgc cccgccacgt ggacacgacg gacaccggcg ggtcgtccta cgtcattcac
     1621 gtcgcccatg ccgcccaggc catcgcgctc ggcaagtgca aggtggcgct gatcacgctg
     1681 gccggacggc cgcggtccga gggcatggcc accggcacgg cgccgcgcgt gcccgctgcc
     1741 gccatgcccg acgcgccgtt cgagttcccc tacgcgcccg ccaccgcgaa catgtacgcc
     1801 atggcggcca tgcgccacat gcaccagttc gggacgacga gcgcccagct cgcctgggtg
     1861 aaggtcgcgg cgtcgcacca cgcgcagcac aacccgcacg cgatgctgcg cgaggtcgtc
     1921 accgtcgagg acgtggtcaa ctcgccgatg atcaccgacc ccctgcaccg gctggactgc
     1981 tgcgtgatca gcgacggcgg tggcgcggtg gtcgtcgtcg caccggagat cgcgcggagc
     2041 ctgcgccgcc cgcgcgtgag ggtgctcggc gccggcgagg ctctcaagct ccagatgggc
     2101 ggcaaggtgg acctcacgta cacgggcgcc gtgtggtccg ggccggcggc gttcgccgag
     2161 gccggcgtca agcccgccga catcaagtac gcgtcgatct acgacagctt caccatcacc
     2221 gtgctcatga cgctggagga cctcggcttc tgcgagaagg gacagggcgg caagttcgtg
     2281 gccgacggca atctcgtctc gggcgtgggc cggctgccct tcaacaccga cggcggcggg
     2341 ctctgcaaca accatcccgc caaccgcggc ggcatgacga aggtgatcga ggcggtgcgg
     2401 cagttgcggg gcgaggcgca cccgaaagtg caggtgaaga actgcgacgt cgcgctcgcc
     2461 cacggcaccg gcgggtcgct gggcacccgg cacgccagcg ccacgctcat catggaaagg
     2521 gagtgacggc catggcgcca gcccagcgga agattccggc gccgcaggtg acgccggaga
     2581 cgaagccgtt ctgggatgcg gcggctgagg gccgcctggt gatcaagcag tgcacggcct
     2641 gcgaccagcc gcaccactac ccgcgcacga tctgcccgtt gtgcggcagc gacgccaccg
     2701 agtggcggcc ggcgtccgga cgcgggacga tctacaccta cagcgtcatg cggcgcgtgc
     2761 ccgagcccta cgccatcgcc tacgtgacgc tcgaggaagg cccgacgatg atgaccaaca
     2821 tcgtcgactg cgacctcgac gcgatccgga tcggtcaggc ggtccgcgtg gtcttcgcgc
     2881 cgagcga
//
LOCUS       DATIAZ010000215         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1768685, whole genome shotgun sequence.
ACCESSION   DATIAZ010000215 DATIAZ010000000
VERSION     DATIAZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768685"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VLF19_07650"
     CDS             complement(<1..594)
                     /locus_tag="VLF19_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93165.1"
                     /translation="MADALLEVRGLTKTFGGLVATSRVDLDVLAGETHAVIGPNGAGK
                     TTLIAQLAGELPPDAGAIRFGGRDVTTLPAPARSALGLARSFQITSIFGDFTALDNVA
                     LAVQAHAGHSFRFWRPARAERELREPARAMLAAVGLGDRAHVPAAALAHGEQRQLEIA
                     MTLATDPSVLLLDEPLAGMGPEETLHMTALLRQLARDH"
     gene            complement(587..1531)
                     /locus_tag="VLF19_07655"
     CDS             complement(587..1531)
                     /locus_tag="VLF19_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93166.1"
                     /translation="MTRSPRALALGAGLVALALVPAAAATLNQPFYLDLVRRIMIFAI
                     AALSLDLILGYGGMVSFGHAAYLGIGAYAVGVLAHHGVHDGFVQWGLAVAASALVALG
                     IGAVSIRTSGVYFIMITLAFTQMLYYLGISIEEYGGDDGMRLAARSQFRLIDLRDGVT
                     FYYVVLAILLLCLLLGHRLVNARFGMVIRSVKSNEARARAIGFSPYRYKLVAFVVAGA
                     MCGVAGALLVNHTEYLTPEFMHWTRSGEIMFMVILGGMGTLTGPVLGAVVLLLLEDVL
                     SAWTVHWQIVLGPFLVLVVLFARRGLAGLLPRAAAPHG"
     gene            complement(1531..2454)
                     /locus_tag="VLF19_07660"
     CDS             complement(1531..2454)
                     /locus_tag="VLF19_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93167.1"
                     /translation="MTLLLVLEQALNGLQYGVTLFLMAAGLTLVFGIMNLVNLAHGSL
                     YMVGAYLATAFHQWTGSFLAGVALALPATLIVGVAVEVVALRTLYERDHLDQVLATFG
                     LILFFNELVAIVWGRAALYTSLPGWLSGHVLVLPGVRYPVYRVVVLVTGLAVGVALWA
                     LVSRTRAGMLIRAGASNRAMVGALGVNIRRLYTLVFGLGAALAGLAGLMAGPIYAVQP
                     GMGEQILIPVFVVIVIGGIGSVRGALVGAVVVGMVDTLGRAFLKPVMATFLRPDVADS
                     AGPALASMLMYMLMAGVLAVRPQGLFPARGR"
BASE COUNT          447 a          888 c          938 g          295 t
ORIGIN      
        1 atggtcgcgc gcgagctgac gcagcagcgc cgtcatgtgc agcgtctctt ccggccccat
       61 gccggccagc ggctcgtcca gcagcagcac ggaaggatcg gttgccagcg tcatcgcgat
      121 ctcgagctgg cgctgctcgc cgtgggcgag ggccgcggcg ggcacgtgcg cgcggtcgcc
      181 gagccccacc gcggccagca tcgcgcgcgc aggctcgcgc agctcgcgct cggcgcgcgc
      241 cggccgccag aaccggaagc tgtgcccggc gtgcgcctgc acggcgagcg cgacgttgtc
      301 gagcgcggtg aaatccccga agatgctggt gatctggaac gagcgcgcca ggcccagcgc
      361 cgagcgggcg ggggcaggca acgtggtgac gtcgcgcccg ccgaagcgaa tggcgccggc
      421 gtcgggcggc agctcgccgg cgagctgcgc gatcagcgtg gtcttgccgg cgccgttcgg
      481 gccgatgacc gcgtgggtct cgccggcgag gacgtcgagg tccacccggc tggtggccac
      541 gaggcccccg aacgtcttcg tcagcccgcg gacctcgagc agcgcgtcag ccatgcggcg
      601 ccgcggcgcg gggcaggagc ccggcgaggc cgcgcctggc gaagaggacg acgaggacga
      661 ggaaggggcc gaggacgatc tgccagtgaa cggtccaggc cgacagcacg tcctcgagca
      721 gtagcaacac cacggctccc agcacgggtc cggtgagcgt ccccatgcct cccaggatga
      781 ccatgaacat gatctcgccg gagcgcgtcc agtgcatgaa ctccggcgtc aggtactcgg
      841 tgtggttgac gagcagcgcg ccggccacgc cgcacatggc gccggcgacg acgaaggcca
      901 cgagcttgta ccggtacggc gagaacccga tggcccgggc ccgggcctcg ttggacttca
      961 cggaccggat caccatgccg aaccgtgcat tgacgaggcg gtggccgagc agcaggcaca
     1021 gcaggaggat cgcgaggacg acgtagtaga acgtcacgcc gtcgcgcagg tcgatcaggc
     1081 ggaactggct gcgggcggcc aggcgcatgc cgtcgtcgcc gccgtactcc tcgatgctga
     1141 tgccgaggta gtagagcatc tgggtgaagg ccagcgtgat catgatgaag tacacgccac
     1201 tcgtccggat ggagacggcg ccgatcccga gggccaccag cgcggaggcg gcgacggcga
     1261 gcccccactg cacgaagccg tcgtggacgc cgtggtgggc gagcacgccg accgcgtagg
     1321 cgccgatccc aagataggcc gcgtggccga aggacaccat gccaccgtag cccaggatca
     1381 ggtcgaggct gagcgcggcg atggcaaaga tcatgatccg gcggacgagg tccaggtaga
     1441 agggctggtt caacgtcgcg gccgcggcgg gtacgagggc gagggccacg agcccggctc
     1501 ccagcgcgag cgcgcgcggc gatcgggtca tcagcggccg cgggccggga agagcccctg
     1561 cggccggacg gccagcaccc cggccatgag catgtacatc agcatcgagg ccagcgccgg
     1621 gccggcgctg tcggcgacgt cgggacgcag gaacgtggcc atcaccggct tgaggaaggc
     1681 gcgcccgagc gtgtccacca tgcccaccac gacggcgccg acgagggcac cgcgcacgga
     1741 gccgatgccg ccgatgacga tgaccacgaa gaccgggatg aggatctgct cgcccatgcc
     1801 cggctggacg gcgtagatcg ggcccgccat cagcccggcc agcccggcca gcgccgcgcc
     1861 gagcccgaac acgagcgtgt acaaccggcg gatgttcacg ccgagcgcgc cgaccatcgc
     1921 gcggttgctg gcgccggcgc ggatcagcat gcccgcgcgc gtccgcgaca ccagcgccca
     1981 cagcgccacc cccaccgcga gcccggtcac gaggacgacc acgcggtaga cggggtagcg
     2041 cacccccggc agcacgagca cgtgtcccga cagccagccg ggcaggctcg tgtacagcgc
     2101 cgcgcgcccc cagacgatcg cgacgagctc gttgaagaac aggatcagcc cgaacgtggc
     2161 gaggacctgg tcgaggtggt cgcgctcgta cagcgtgcgc agggcgacga cttccacggc
     2221 cacgccgacg atcagcgtcg ccggcagcgc cagcgccacc ccggcgagga acgagcccgt
     2281 ccactggtgg aacgcggtcg ccaggtaggc gcccaccatg tagagggagc cgtgggcgag
     2341 gttgacgagg ttcatgatgc cgaagacgag cgtgaggccg gccgccatga gaaagagcgt
     2401 cacgccgtat tgcaggccgt tcagcgcctg ctccagcacc agcaggaggg tcatcggcgc
     2461 gtccgcataa agaaaagggg cgcggtggac ccgcgcccct tcctcacccc ctggggatca
     2521 ctaccacttc atcccacagt ccttggagta ggcgtccttg tggttctc
//
LOCUS       DATIAZ010000216         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_321845, whole genome shotgun sequence.
ACCESSION   DATIAZ010000216 DATIAZ010000000
VERSION     DATIAZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321845"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..1622)
                     /locus_tag="VLF19_07665"
     CDS             complement(375..1622)
                     /locus_tag="VLF19_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HSE93168.1"
                     /translation="MSGASIEETLDLWSAELREVKAQIQPLFGHASVAASASAFLDGL
                     LGPERRKTGWMRAEAAGDPGPWRQQAVLGRSRWEADALRELVREYVVETLAAPDAVLV
                     IDETGFLKQGKASCGVGRQYTGSAGKITNCQIGVFAAYVSAKGYAFIDRRLYLPKDWT
                     DAPARLAAAHVPAKVGFATKPRLAVGMIERAIRAGVPFAWAAADSIYGVGEVEGVLRQ
                     AGKGYVLGVTGTHPFRSWGKEPGWSGTAATIAARLPTGAYVRLSAGEGTKGPRLYDWA
                     YLELADLEAQDVGYSGGPDVWTRGLLIRRSLADQSLTFFTTWCPKGTPLEVLVRVEGC
                     RWAVEDGFETAKTELGLDHNETRSWHGWHRHVSLVMLAFAVLAVIRHRANTPLSPPKS
                     AAAERCRRWSAGLSRRSVGSPAG"
     gene            complement(1707..2882)
                     /locus_tag="VLF19_07670"
     CDS             complement(1707..2882)
                     /locus_tag="VLF19_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HSE93169.1"
                     /translation="MGRAGVLQEVRVMRFAEVVGRLYDGRLSCEEAADVLGMSVSSFY
                     RWRRRFEAEGGEGLADGRLGRASGRRAPVDEVTRVLELFETRYFDFNVKHFHEKLTAE
                     HGLRRSYSWTKNTLQAAGKVRRAGRRGAHRRRRPRKPMIGMMLHQDGSRHEWVPGHWW
                     DLIVTMDDADSTIYSAFFVEEEGTMSSLRGIRQVIERHGLFGSLYADRGSHYWTTTAA
                     GKIDRDNPTQVHRALKQLGIELIPAYSPEARGRSERMFGTLQGRLPQELRVKGIADLD
                     AANRFLAEVFIPEHNRRFREAPAEAGGAFVPWAGRSLADILCVQEERTVGNDNTVRHQ
                     RRILQIPADKHRHHYVRCKVRVHEYPDGALAVFHGPRCLARYDADGTPIQQDQKTAA"
BASE COUNT          476 a         1104 c          999 g          463 t
ORIGIN      
        1 aggcggccgc cgaagccgcc accgagggat gcgcgaacag cggccggagc cgcgccttga
       61 gcccgcccag ctcggccgac cacagaccca gcatttgctc caccgaaacc ccggacaccg
      121 ctgcgtctcc gcttcaggcg cagatagatg gagacgctca ggtgcaactg taatggtagg
      181 ccaggacgtc gcccgcgggc tcggcccggc tcacggcggc gtgagcgagt aacccaaagg
      241 tagctacgat gatcgcgcgg cctagcacta cagttgcagg gcctgcttga ggtgggcttg
      301 ctgcgccacg gcctgatggg ctcgtcgcca gacggaccaa gcgatgatga aggccggctc
      361 gatccggcgc tgggctaacc ggcaggcgag ccgacggatc tcctggatag accagcggac
      421 catcgacggc accgctcggc ggccgcgctt tttgggggtg acaggggggt gttcgcccga
      481 tggcggatca ccgctagcac ggcgaaggcg agcatgacca acgaaacgtg gcggtgccag
      541 ccatgccagc tgcgcgtctc gttgtgatca agcccgagtt cggtcttggc ggtctcgaag
      601 ccgtcctcga ccgcccagcg gcagccctcg acccggacca gcacctcaag cggcgtgccc
      661 ttcgggcacc aggtggtgaa gaacgtcagg ctctggtcgg ccaggctgcg ccggatgagc
      721 agaccccggg tccacacgtc gggaccaccg gagtagccca cgtcttgcgc ctccaggtcg
      781 gccaactcca ggtaagccca gtcgtagagc cgcggcccct tggtgccttc ccccgccgag
      841 agacgaacgt aggccccggt cggcaacctg gccgcgatcg tcgccgccgt gccgctccag
      901 ccgggctcct tgccccagga ccggaacgga tgcgtgccgg tgacgccgag cacgtacccc
      961 ttgcccgctt ggcggagcac cccctccacc tcgcccacac catagatgct gtccgcggcc
     1021 gcccaggcga acggcacgcc tgcccggatc gcccgctcga tcatgccgac cgcgagccgc
     1081 ggcttggtgg cgaagcccac cttcgccggc acgtgggccg cggccaagcg ggcgggggca
     1141 tcggtccagt ccttgggcag atagagccgg cggtcgatga aggcatagcc cttggcggag
     1201 acgtaggccg cgaacacccc gatctgacag ttcgtgatct tccccgccga gccggtgtat
     1261 tgccgtccga ccccgcagga cgccttgcct tgcttgagaa agccggtctc atcgatcacc
     1321 agcaccgcat ccggcgcggc cagggtctcg accacgtact cgcgcaccag ctcgcgcagt
     1381 gcgtcggcct cccagcggct gcgccccagc acggcctgct gccgccacgg cccgggatcg
     1441 cccgccgcct cggcgcgcat ccagccggtc ttgcgccgct ccggcccgag cagcccgtcc
     1501 aggaaagccg aggcggaggc cgccacactg gcatgcccga acaggggttg gatctgtgcc
     1561 ttgacctcgc gcagctcggc cgaccagaga tccagcgtct cttcaattga tgcacccgac
     1621 atcggcaact ccatccatct cgggagagat cagatggggc ggcccaggtg caactgtagt
     1681 gctagagcgg cgcgcgtcgg gcggcgtcac gcggccgttt tctggtcctg ttggataggc
     1741 gtgccgtcgg cgtcgtaacg ggccaagcaa cgcggcccgt ggaagacggc gagcgcgccg
     1801 tcgggatact cgtgcacccg gaccttgcac ctgacgtaat gatgccggtg cttgtcggcc
     1861 gggatctgga ggatgcgtcg ctggtggcgc accgtgttgt cgttgccgac ggtgcgctcc
     1921 tcctggacgc agaggatgtc ggccagactt cgccccgccc acggcacgaa cgcgccgccg
     1981 gcttctgccg gcgcctcgcg gaagcggcga ttgtgctcgg ggatgaagac ctcggcgagg
     2041 aagcggttgg ccgcatccag gtcggcgatg cctttcaccc gtagctcctg cggcagccgc
     2101 ccctgcaagg tgccgaacat ccgctccgag cggccccggg cttcgggcga ataggccggg
     2161 atcagctcga tgccgagctg cttcaaggca cggtggacct gggtcgggtt gtccctgtcg
     2221 atcttccccg ccgccgtcgt cgtccagtag tgcgacccgc gatcggcgta gagcgagccg
     2281 aacaggccgt gccgctcgat cacctggcgg atgccgcgga gcgagctcat cgtcccttcc
     2341 tcttcgacga agaaggccga gtagatcgtg ctgtcggcat cgtccatggt gacgatcagg
     2401 tcccaccagt gccccggcac ccattcgtgg cgggagccgt cctggtgcag catcatgccg
     2461 atcatcggct tgcgcggccg gcgccgccgg tgggcgccgc ggcgcccggc gcggcgaacc
     2521 ttgccggccg cctggagcgt gttcttggtc cagctgtagc tgcgccggag gccgtgctcc
     2581 gccgtcagct tctcgtggaa gtgcttcacg ttgaaatcga agtagcgcgt ctcgaacagc
     2641 tccagcaccc gcgtcacctc gtccaccggc gcccgccgcc ccgaggccct gccgagcctg
     2701 ccatccgcca agccctcccc gccctctgcc tcgaagcgcc gccgccagcg atagaacgag
     2761 ctgaccgaca tccccagcac gtcggccgcc tcctcacagg acaggcgacc gtcgtaaagt
     2821 cgtcccacga cctccgcgaa ccgcatcacg cggacctcct gcagcactcc ggcccgaccc
     2881 atccatgccc ccctccctgg agagcttcaa agatcggtca gaccgtgtgc tacctggatc
     2941 ggtcacatca tgtgctacgg acagggggcg ggcgccgcgc ggcctcgccg accggcggca
     3001 tcttgaacat cgcggccttc ccggtgacca tgaggtgccc cc
//
LOCUS       DATIAZ010000217         2885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_978683, whole genome shotgun sequence.
ACCESSION   DATIAZ010000217 DATIAZ010000000
VERSION     DATIAZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978683"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VLF19_07675"
     CDS             complement(<1..858)
                     /locus_tag="VLF19_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HSE93170.1"
                     /translation="MRRLTVLACLLAIGLAASAGFAAYPERPVKLIVPWAAGGDTDAI
                     FRPFGQHLQKHLGQTVVIANVTGASGTAGAREAKGSPPDGYTLYAVHDYIHSTYYTGV
                     ADVNYTDFEAICSVSNTPSVLTASPKTKWSSWQELLSDAKARPGQITVGATLGSTSHF
                     FPALIEKAANIKFKYVSYEGLAPRMNAILGGHIDLTDSNLTQKGKVEAGQLKFLAIAT
                     EKRLPDAPSVPTLKELGVNVVYAVTRGLMVPKGTPQEVVARIEDACKKATSEPKFAED
                     MAKQGTFVRF"
     gene            complement(1026..>2885)
                     /locus_tag="VLF19_07680"
     CDS             complement(1026..>2885)
                     /locus_tag="VLF19_07680"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HSE93171.1"
                     /translation="GPRVPERVIVIVGDAELDEGNVWEALAEEAVGRLGEVLWIVDVN
                     RQSLDRIVPDARVRQLPALFEAFGWRVIELRWGRRLGALFSRPGGAALRARLTAMPYA
                     EYQGLLRLPAAAVRKTLASGDEAAVIDRRLADVPDDALPALVADLGGHDLAAILDALD
                     EAWRERGRPTVILAHTIKGWGLPLAGDPLNHTALLTSTQIEALRASLGVAAGAEWDGF
                     GPDSEEAAWIQRLPPLFAPPPPAPPPSVPAEIAETYPDQCSTQEAFGRVLGRLARQPV
                     ADAIVTVSADVAVTTHLAGWINRRGVWFPETKPNYCGDVPSPVQWRESERGQHVELGI
                     AEHNLFLLLGALGLTRELSGITLLPIGTLYDPFVTRGLDAFYHALYSGSRFVVVATPS
                     GVSLSHEGGAHQSVITPGIGVALPDCAYFEPAFAREVEWVLLAALADIARREGESLYL
                     RLSTRPVDQRLAPPDHEALRADVLAGGYRLVDARGAAGWDAETNAVSIFAVGAMVTEA
                     VSAGRLLREHGINASVFVVTSPDRLYRGLDAPRPHLERLVSADEEGVPVVSVLDGHSH
                     ALAFVGGALGVAQIALGVDHFGQSGAPADLYRHYGVDGPAIARAARLLVGETE"
BASE COUNT          423 a         1036 c         1037 g          389 t
ORIGIN      
        1 gaaacgcacg aacgtaccct gcttggccat gtcctcggcg aacttcggct ccgaggtcgc
       61 cttcttgcag gcgtcctcga tcctggcgac cacctcctgg ggcgtgccct tcggcaccat
      121 gagcccacgg gtcacggcgt acacgacgtt cacgcccagc tccttgaggg tgggcacgct
      181 gggggcgtcg ggcagccgct tctccgtggc gatggccagg aacttgagtt gcccggcctc
      241 caccttgccc ttctgcgtca ggttcgagtc cgtgaggtcg atgtggcctc cgaggatggc
      301 gttcatgcgg ggcgccaggc cctcgtagga gacgtacttg aacttgatgt tcgcggcctt
      361 ctcgatgagc gccgggaaga agtggctggt ggagccgagc gtggcgccca cggtgatctg
      421 ccccggccgc gccttggcgt cggacagcag ctcctgccag ctcgaccact tggtcttcgg
      481 gctcgccgtg aggaccgacg gcgtgttgga caccgagcag atcgcctcga agtcggtgta
      541 gttcacgtcg gccacgcccg tgtagtaggt cgagtggatg tagtcgtgca cggcgtagag
      601 cgtgtagccg tcgggaggcg agcccttggc ctcgcgcgcc cctgccgtcc ccgaggcgcc
      661 ggtgacgttg gcgatgacga cggtctgtcc gaggtgcttc tgcagatgct gcccgaaggg
      721 ccggaagatc gcgtcggtgt cgccgccggc ggcccagggc acgatgagct tcacgggacg
      781 ctcgggatag gcggcgaagc ccgccgaagc ggcgagccca atggcgagca gacaggcaag
      841 cacggtcagt cggcgcatgt gaatctcctt tcgcggacgg ctacatatgc gggcggccaa
      901 atgataacag agccccgtcg cgcccgggcc gaccgcgtgc ccaccccgcc gatgccgcgc
      961 gccccgatgt cgtgcccacc cgcccgcagc cgcgcgacgg cccaaggggc gcgcgacgcg
     1021 agagactatt cagtctcgcc gacaagcagc cgcgccgccc gcgcgatggc cggcccgtcg
     1081 acgccgtagt gtcggtagag atcggccggc gcgcccgact ggccgaagtg atccacgccg
     1141 agcgcgatct gggccacgcc cagcgcgccg ccgacgaacg cgagcgcgtg cgagtggccg
     1201 tcgagcacgg agaccaccgg cacgccttcc tcgtcggcgc tcaccagcct ctccagatgg
     1261 ggccgtggcg cgtcgagccc gcggtagagc cggtcggggc tcgtcacgac gaagacgctc
     1321 gcgttgatcc cgtgctcgcg cagcaggcgc ccggccgaga cggcctccgt caccatcgcg
     1381 cctaccgcga agatcgacac ggcgttcgtc tcggcgtccc atccggccgc gccgcgggcg
     1441 tccacgaggc ggtagccgcc ggccagcacg tcggcgcgga gcgcctcgtg atccggcggg
     1501 gccaggcgct ggtccacggg ccgcgtcgac agccgcaggt agaggctctc gccctcgcgc
     1561 cgcgcgatgt ccgcgagcgc ggccaggagc acccactcga cctcgcgcgc gaaggcgggc
     1621 tcgaaatacg cgcagtcggg cagcgccacg ccgatccccg gcgtgatcac ggactggtgc
     1681 gcgccgccct catgcgagag gctcacgccc gagggcgtgg ccaccaccac gaagcgcgag
     1741 cccgagtaga gcgcgtgata gaacgcgtcg aggccgcggg tgacgaacgg gtcgtagagg
     1801 gtgccgatcg gcagcagggt gatgccggac agctcgcgcg tgagcccgag cgcgccgagc
     1861 agcaggaaca ggttgtgctc ggcgatgccc agctccacgt gctgcccgcg ctcgctctcg
     1921 cgccactgca cgggcgaggg aacgtcgccg cagtagttcg gcttcgtctc gggaaaccac
     1981 acgccccggc ggttgatcca gccggccagg tgcgtcgtga ccgcaacgtc ggcggagacg
     2041 gtcacgatgg cgtcggcgac gggctgcctc gcgaggcggc cgagcacgcg gccgaacgcc
     2101 tcctgcgtcg agcactgatc ggggtacgtc tcggcgatct ccgccggcac cgagggtggc
     2161 ggcgcggggg gcggaggggc gaacagcggc ggcagccgct ggatccacgc cgcttcctcg
     2221 ctgtccgggc cgaagccgtc ccactcggcc cccgccgcca cgccgagcga cgcgcggagc
     2281 gcctcgatct gggtcgacgt gagcagcgcg gtatggttca gcggatcgcc ggcgagcggc
     2341 agcccccatc ccttgatcgt gtgggccagg atcacggtcg gacggccacg ctcgcgccac
     2401 gcctcgtcca gcgcgtcgag gatggccgcg aggtcgtgac caccgagatc ggcgacgagc
     2461 gcgggcagcg cgtcgtccgg cacgtcggcg agccgtcggt cgatcaccgc ggcctcgtcc
     2521 cccgatgcga gcgtcttgcg caccgcggcc gccggcagac gcagcaggcc ctggtattcg
     2581 gcgtagggca tcgccgtgag gcgcgcccgt agcgcggctc cgcccgggcg cgagaacagc
     2641 gccccgaggc ggcggcccca gcgcagctcg atcacccgcc agccgaaagc ctcgaagagc
     2701 gcggggagct gccggacgcg cgcatcgggc acgatgcggt cgagactctg gcggttgacg
     2761 tccacgatcc agagcacctc gcccagcctg ccgaccgcct cctcggcgag agcttcccag
     2821 acgttgccct cgtccagctc ggcgtcgccc acgatgacga tcacgcgctc cggaacacgc
     2881 ggccc
//
LOCUS       DATIAZ010000218         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1505860, whole genome shotgun sequence.
ACCESSION   DATIAZ010000218 DATIAZ010000000
VERSION     DATIAZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505860"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VLF19_07685"
     CDS             complement(<1..281)
                     /locus_tag="VLF19_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93172.1"
                     /translation="MTSSPSLRVLSLMVATGLVACTVGFAGASAMAPGTTQVAAQAGG
                     GTTAPDVPGPLQKRHGRWQARLQQHLGLTDDQANQIGQVFQRNMQAARP"
     gene            complement(448..1950)
                     /locus_tag="VLF19_07690"
     CDS             complement(448..1950)
                     /locus_tag="VLF19_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE93173.1"
                     /translation="MPHYLSPDELHRTAPAETAAFASPVPTQIVSNGEYNPLPQTREQ
                     RRMEARIRALADDLGPRHGLSRRAFLASTAGMAAAFLAMNEVFGPLFDVSRAEAAQPG
                     VAQARADALAGQLIIDCQTHFVRDDFRQDGLLDLAKYAKQHWNPALAGENTLARFKFE
                     NYVKEVFVDSDTKVALLSGAPFDDQSWDLLSNDQIAAARAAINRIAGSRRMLAHAVFT
                     PKRAGWMAEVDRAIATLKPDSWKGYTIGDPLFPSKLGSYWRLDDEKLVYPFYEKIVKA
                     GTNTVCIHKGLLPADYEKSWPGVWQHAAVGDLGKAAKDWPQINFVIYHSALRAFLESP
                     AAVLSEFEQTGRIKWATDLAEIPAKYGVRNVYGEIGTSFATCAVANPRVAAALVGTLI
                     RGLGADRVLWGSDSVWYGSPQWQIEALRRLEIPEDMRRKHGFTPLGPADGSVKSAIFA
                     SNAAALYGVHVKAALGDITGDKIAALRAAYVAEGGQRSNLRYGYIHRPGA"
     gene            complement(1995..2453)
                     /locus_tag="VLF19_07695"
     CDS             complement(1995..2453)
                     /locus_tag="VLF19_07695"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSE93174.1"
                     /translation="MSLEQELNDTLKRAMLGKDQRTADVVRMVKTRLQERRTAKGFAG
                     AVDDALVRDVIAAYRKQLQKALGDYEQAGDRGAAQAGQLRFEIELLERWLPRGLDEAA
                     LRALVQERIAALGISDAKQVGRLIGDVMKTHKGRVEAADVKRIAEELLRA"
     gene            complement(2563..>3393)
                     /locus_tag="VLF19_07700"
     CDS             complement(2563..>3393)
                     /locus_tag="VLF19_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE93175.1"
                     /translation="AIAEGRTAPADFPPTCPDWTEEEAINRAILIGRLTGCPTYVVHL
                     STMVGLERIKRAQAEGQKVWCETCPQYLLLTDEEMTRWGPFAKIGPPLRPAHGPDRAA
                     LWAGVEQGFIASVASDHSPRAPAAKEPGRRNIFVDEQGRPIPFGAPSLETLVPLVWSE
                     GVGKRGLPPTWMARVMAENPARIFGLWPRKGAIRVGADADLTIWDPEPEWTIDRSQHL
                     GVAGFTPYQGWKVRGRPWMTLLRGQTLLNPQGEVEQKPGYGRYLPRTGPTPPLAGAVR
                     "
BASE COUNT          426 a         1187 c         1235 g          545 t
ORIGIN      
        1 tggggcctcg cggcttgcat gttgcgctgg aagacctgcc cgatctggtt ggcctggtcg
       61 tcggtgagcc cgaggtgctg ctgcagacgc gcctgccagc ggccgtgtcg cttctgcagc
      121 ggccccggga cgtcgggggc ggtggtgcca cctcccgcct gtgcggcgac ctgggtcgtg
      181 ccgggcgcca tggcactcgc ccctgcgaac ccgaccgtgc acgcgacgag gccggtggcc
      241 accatcagac tcagaacgcg gagcgaaggg ctcgaggtca tcgatgtctc ctccatctgg
      301 cggctcggcc gtttgccggc tgccatcccg tgagacgggc gcacgctgcc gggtatttac
      361 gcgcgcggcg aggggaggcc tgggaattga ggcggggcgc cgcctgccgg gcgcacgccc
      421 cgcttcgcgc tcacgtcgcg acggcgctca tgcgcccggt cggtggatgt aaccgtaccg
      481 caggttgctc cgctgcccgc cctcggcgac gtacgcggcg cggagggcgg cgatcttgtc
      541 gcccgtgatg tcgccgagcg cggccttcac gtgcacgcca tacagggccg cggcgttgct
      601 cgcgaagatg gcgctcttca cggagccgtc ggccggaccc agcggtgtga agccgtgctt
      661 gcgccgcatg tcctcgggga tctcgagccg ccggagcgcc tcgatttgcc actgcggcga
      721 gccgtaccag acggagtcgc tgccccacag cacgcggtcg gcgccgagcc cgcggatcag
      781 ggtgcccacg agcgcggcgg caacgcgtgg gttggcgacc gcgcacgtgg cgaaggacgt
      841 gccgatctcg ccgtagacgt tgcgcacgcc gtacttggcc gggatctccg ccaggtcggt
      901 ggcccacttg atccgcccgg tctgctcgaa ctcggaaagc acggcggcgg gggattcgag
      961 gaaggcgcgg agagccgagt ggtagatcac gaagttgatc tgcggccagt ccttcgccgc
     1021 cttgccgaga tcgccgacgg cggcgtgctg ccatacgccg ggccacgact tctcgtagtc
     1081 cgccggcagg agccccttgt ggatgcacac ggtgttggtg ccggccttga cgatcttctc
     1141 gtagaagggg tacacgagct tctcgtcgtc gagccgccag taggagccga gcttcgaggg
     1201 gaagagcggg tccccgatcg tgtagccctt ccacgagtcg ggcttcaggg tggcgatcgc
     1261 gcgatcgacc tccgccatcc atcccgcgcg cttgggggtg aagaccgcgt gggcgagcat
     1321 ccgccgggag ccggcgatgc ggttgatggc cgcgcgcgcc gccgcgatct ggtcgttgct
     1381 gagcaggtcc cacgactggt cgtcgaacgg cgcgccggag agcagggcca ccttcgtgtc
     1441 gctgtcgacg aagacctcct tgacgtagtt ctcgaacttg aagcgggcca gggtgttctc
     1501 gccggcgagc gccgggttcc agtgctgctt ggcgtacttg gccaggtcga ggaggccgtc
     1561 ctgcctgaaa tcgtcgcgaa cgaagtgcgt ctggcagtcg atgatgagct ggccggccag
     1621 cgcgtcggcg cgggcctggg ccacgccggg ctgcgcggcc tcggcgcggc tcacgtcgaa
     1681 gagcgggccg aagacctcgt tcatggccag gaacgccgcc gccatgccgg cggtgctggc
     1741 gaggaaggcg cggcggctca ggccgtgccg cgggccgagg tcgtcggcca gggccctgat
     1801 gcgggcttcc atccgccgct gctcgcgcgt ctgcggcagc gggttgtact cgccgttcga
     1861 gacgatctgg gtcgggaccg gcgaggcgaa ggccgcggtc tcggcggggg cggtacggtg
     1921 cagctcgtcc ggactgagat agtggggcat gcggcacctc cgtgtgctcg cggttcggga
     1981 cggaggaggc gattctacgc ccgcagcagc tcctcggcga tccgcttgac gtcggcggcc
     2041 tcgacccgtc ccttgtgcgt cttcatgacg tcgccgatca ggcgtccgac ctgcttcgcg
     2101 tcgctgattc cgagcgccgc gatgcgctcc tgcaccagcg cgcgcagcgc cgcctcgtcg
     2161 aggcccctgg gcagccagcg ctcgaggagc tcgatctcga accgtagctg cccggcctgc
     2221 gcggcgccgc ggtcacccgc ctgctcgtag tcgcccaggg ccttctgcag ctgcttgcgg
     2281 taggcggcga tgacgtcgcg caccagcgcg tcgtccacgg ccccggcgaa gcccttggcc
     2341 gtgcgccgct cctgcagcct ggtcttgacc atgcgcacga cgtcggcggt gcgctggtcc
     2401 ttgcccagca tcgcccgctt gagcgtgtcg ttcagctcct gctcgaggct catcgccgcc
     2461 tcggcgcgct cacggcttca ccgggggata gctgcggtcg tacacgggcg tcttgaggaa
     2521 ctcctcgggc acggtcccga cggcgtcgtc acgtctggcg cctcatcgca cggcgccggc
     2581 caggggcggc gtcgggccgg tgcgtggcag gtagcgtccg tagccgggct tctgctcgac
     2641 ctcgccctgc gggttcagca gcgtctggcc gcggagcagc gtcatccacg gccggccgcg
     2701 caccttccag ccctgatacg gcgtgaagcc cgcgacgccg aggtgctggg acctgtcgat
     2761 cgtccactcg ggctcggggt cccagatcgt gaggtcggcg tctgcgccga cgcggatcgc
     2821 gcccttgcgc ggccagagcc cgaagatccg cgcgggattc tcggccatca cccgcgccat
     2881 ccaggtcggc ggcagcccgc gcttgcccac gccctcgctc cacacgaggg ggaccagcgt
     2941 ctcgagcgac ggcgcgccga acgggatcgg gcgcccctgc tcgtccacga agatgtttcg
     3001 ccgccccggc tccttcgccg ccggcgcccg cggcgagtga tcgctcgcga cactggcgat
     3061 gaagccctgc tcgacgccgg cccagagtgc ggcgcggtcg ggaccgtgcg ccggccgcag
     3121 cggcggtccg atcttcgcga aggggcccca ccgcgtcatc tcctcgtcgg tgagcagcag
     3181 gtattgcgga cacgtctcgc accacacctt ctggccctcg gcctgcgcgc gcttgatgcg
     3241 ctcgagcccg accatcgtgg agaggtggac gacgtacgtc ggacagccgg tgagccggcc
     3301 gatgaggatg gcgcggttga tcgcctcctc ctccgtccag tcgggacacg tcggcggaaa
     3361 gtcggccggc gccgtccggc cctcggcgat cgc
//
LOCUS       DATIAZ010000219         5673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_455575, whole genome shotgun sequence.
ACCESSION   DATIAZ010000219 DATIAZ010000000
VERSION     DATIAZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455575"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VLF19_07705"
     CDS             complement(<1..426)
                     /locus_tag="VLF19_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HSE93176.1"
                     /translation="MPTDSELPFADDLGPAKIVHVHEPTLGLEAVLVVDNVARGPAIG
                     GLRMAPDVSTEECVRLARAMTLKNAAAGLPHGGGKSVLRGDPRMAADRKERLIRAFAH
                     ALRHEDDYIFGPDMGTDETCMAWVKDEIGRAVGLPPALGG"
     gene            complement(458..616)
                     /locus_tag="VLF19_07710"
     CDS             complement(458..616)
                     /locus_tag="VLF19_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93177.1"
                     /translation="MTLVKIDHPRRIDRVIDYFGQPRRAGSMIRRRTGAPPQARVSRA
                     GAKRHTAA"
     gene            complement(633..3056)
                     /gene="ppsA"
                     /locus_tag="VLF19_07715"
     CDS             complement(633..3056)
                     /gene="ppsA"
                     /locus_tag="VLF19_07715"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HSE93178.1"
                     /translation="MGSPPPGAPSYVRWFRDIGASDVPVVGGKTASLGELYTRLRAAG
                     VRVPDGFAITAGAYDALLDVGGLRSRIAKRLTGVDGRDLDALAEAGADIRAWIRSAPV
                     PADLEAQIAEAYRTLAGGPEPTLAVAVRSSATAEDLPEASFAGQQETYLGVRGGAALL
                     GACRRCFASLFTDRAISYRIHHGFDHMAVRLSIAVQKMVRADLGAAGVMFTLDPESGF
                     RDVVVIDAAYGLGEAVVGGRIDPDEFWVFKPTLHLGDRAILRRTVGRKEWKIVTEAGA
                     TRRVDVAPDARARPSLDDADVVELARFGVAIEAHYGRPMDIEWAKDGEDGRLYILQAR
                     PETVHRAERRAVVDVFELAPGARRERLVTGRAVGQKIGSGRARLLRDARALEAFEPGD
                     VLLAPVTDPDWEPIMKRAAAIVTDRGGRTCHAAIVSRELGVPCVVGTERGTEVIGDGE
                     TVTVSCAEGETGVVYRGAVPFERRPVDLAALPWPATAVMLNVGSPAEAFRMAALPNAG
                     VGLARIEFIIANTVRVHPLALLHPERVTEPAARALIEELTRGHADRGEYFVARLAEGV
                     ATIAAAFHPKDVIVRLSDFKSNEYAGLVGGRAFEPVEENPMLGFRGAFRYCHPRYEEA
                     FALECRAMRRVREEMGLLNVKLMVPFCRTPEEGRRVLEAMARHGLRREENDLEVYVMC
                     EIPSNVLLAPAFAQLFDGFSIGSNDLTQLVLGVDRDSELVAPVFDERNPAVLTMVGDV
                     IAAARRAGRKVGICGQAPSDYPEFTRFLVQRGITSISLNADALVRGIETVATAEAELR
                     REVVCPARP"
     gene            complement(3056..3256)
                     /locus_tag="VLF19_07720"
     CDS             complement(3056..3256)
                     /locus_tag="VLF19_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93179.1"
                     /translation="MTLTSDRIRGRLVEVEVGEVQGLGWTAVGVVKNGLAPEVGLRFQ
                     ATAPTSEEAARRLQAEIEAYFA"
     gene            complement(3434..3919)
                     /locus_tag="VLF19_07725"
     CDS             complement(3434..3919)
                     /locus_tag="VLF19_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE93180.1"
                     /translation="MKAKRILVSLDGSRVAEAALPVALDLAGGAGATLLLLRVIEAPL
                     PGPESAAARLDVTREAETYLDDVARRAREEGARNVTVALWQGSPSQAIVTAADQYQVD
                     LIVMTTHGRTGLQRDCFGSVAESVLRGTGRPVLVVRESATACETPRGHAAQLSVRGSA
                     A"
     gene            complement(4001..4432)
                     /locus_tag="VLF19_07730"
     CDS             complement(4001..4432)
                     /locus_tag="VLF19_07730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSE93181.1"
                     /translation="MKPLPHRYEARIAGGPEGYATLSSAGVPDLRTAPPIDFGGPGDA
                     WSPEQLLIASVEACFLLTFRAIAVASRVEFTSLSVDGEGIVDRSNGRTRFTEIVLRPR
                     VTLPAGVDSIRVRRALEKAERACLVSASLGTPVRLEPELAG"
     gene            complement(4517..4777)
                     /locus_tag="VLF19_07735"
     CDS             complement(4517..4777)
                     /locus_tag="VLF19_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HSE93182.1"
                     /translation="MTREALRATVLHVLGEIAPEADLAALSPGLAFREQLDLDSMDLL
                     NFVVALHGALGVDIPEADYPKLATLDACVEYLEPRTSRASHA"
     gene            complement(4774..>5673)
                     /locus_tag="VLF19_07740"
     CDS             complement(4774..>5673)
                     /locus_tag="VLF19_07740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSE93183.1"
                     /translation="APPAAVAPAPVVHGPAPAGERARISPLARKVAAQLGIDPATLRG
                     TGPGGTVTREDVERVAAARPAAPAPAVAAGRPRLDTARMRQTIAAAMTRAKREIPHYY
                     LAHTVDMHRGLTWLGEENARRPVTDRLLYGVLLLKAVALALREVPELNAVWRGDRAEP
                     SAAVHVGVAISVRGGGLVAPALHDADRRPLGDLMRAFRDLVQRARTGGLRSSELSDPT
                     ITVTSLGEQGVETVLGIVYPPQVAIVGFGRIVERPWVVDGAIVPRPLVTTTLSADHRV
                     SDGHRGGLFLAAVERWLQAPERL"
BASE COUNT          787 a         2097 c         2054 g          735 t
ORIGIN      
        1 cccgcccagg gcgggcggca gccccaccgc gcgaccgatc tcgtccttga cccacgccat
       61 gcacgtctcg tcggtcccca tatcgggacc gaagatgtag tcgtcctcgt ggcgcagtgc
      121 gtgggcgaac gcgcggatga ggcgctcctt ccggtcggcc gccatgcgcg ggtcgccgcg
      181 cagcaccgac ttgccgcctc cgtgcgggag gccggcggca gcgttcttca gcgtcatcgc
      241 gcgcgccagg cggacgcact cctcggtgct cacgtccggc gccatgcgca ggccgccgat
      301 cgcggggccg cgggcgacgt tgtcgacgac gagcaccgcc tccagcccga gcgtcggctc
      361 gtgcacgtga acgatcttcg cggggccgag atcgtccgcg aacgggagct cgctgtccgt
      421 gggcattaca cggcagcgtg aaacgcgcgg gcagtcatca cgctgccgta tgccgcttcg
      481 ccccggcgcg gctcacgcgt gcctgcggtg gcgccccggt acggcggcgg atcatcgatc
      541 cggcgcggcg gggctgaccg aagtagtcga tgacccggtc gatccgacgg ggatgatcga
      601 tcttcacgag cgtcatggat gatctcctgg gctcatggac gagccgggca gacgacctct
      661 cgccgcagct ccgcctcggc cgtcgcgacc gtctcgatgc cgcgcaccag cgcgtcggca
      721 ttgagggaga tgctggtgat gccgcgctgc acgaggaagc gcgtgaattc cgggtagtcg
      781 ctgggcgcct ggccgcagat ccccaccttg cgcccggccc gccgcgccgc ggcgatcacg
      841 tcgcccacca tggtgagcac ggcgggattg cgctcgtcga acaccggcgc caccagctcg
      901 gagtcgcggt cgacgccgag cacgagctgc gtcaggtcgt tggacccgat ggagaacccg
      961 tcgaagagct gggcgaacgc cggggcgagg agcacgttcg aggggatctc gcacatgacg
     1021 tacacctcga ggtcgttctc ctcgcgccgc agaccgtggc gcgccatcgc ctcgagcacg
     1081 cgccgtccct cctcgggagt gcggcagaag ggcaccatca gcttcacgtt gaggagtccc
     1141 atctcctcgc gcacgcggcg catggcccgg cactcgagcg cgaacgcctc ctcgtagcgc
     1201 gggtggcagt agcggaaggc gccgcggaag ccgagcatcg ggttctcctc gacgggctcg
     1261 aacgcgcgcc cgcccacgag gcccgcgtac tcgttcgact tgaagtcgga gagccgcacg
     1321 atgacgtcct tggggtggaa cgccgcggcg atggtcgcca cgccttccgc caggcgggcc
     1381 acgaagtact ccccgcggtc ggcgtggccg cgtgtcagct cctcgatgag cgcgcgcgcc
     1441 gcgggctccg tgacgcgctc ggggtgcagg agcgccagcg ggtggacgcg aacggtgttc
     1501 gcgatgatga actcgatccg ggccaggccg acgccggcgt tcggcagcgc cgccatccgg
     1561 aacgcctccg ccgggctgcc gacgttgagc atcaccgccg tcgccggcca gggcagcgcc
     1621 gcgagatcga ccgggcggcg ctcgaagggc accgcgccgc ggtagacgac gcccgtctcg
     1681 ccctcggcgc aggacacggt caccgtctcg ccgtcgccga tcacctcggt cccccgctcg
     1741 gtgccgacca cgcagggcac gcccagctcg cggctgacga tggcggcatg gcaggtccga
     1801 ccaccgcgat ccgtgacgat ggccgccgcg cgcttcatga tgggctccca gtccgggtcg
     1861 gtcacgggcg ccaggaggac gtcgccgggc tcgaaggcct ccagcgcgcg cgcgtcccgg
     1921 agcagccgcg cccggccgct gccgatcttc tggcccaccg cgcgtcctgt cacgagccgc
     1981 tcgcggcgag ctccgggcgc cagctcgaac acgtcgacga cggcgcgccg ttcggcgcgg
     2041 tgaacggtct ccgggcgggc ctgcaggatg tacaggcggc cgtcctcgcc gtccttggcc
     2101 cactcgatgt ccatcggccg cccgtaatgg gcctcgatag ccacgccgaa gcgggcgagc
     2161 tccaccacgt cggcgtcgtc caggctcgga cgcgcgcgcg cgtcgggggc cacgtcgaca
     2221 cgccgcgtgg cgccggcctc ggtgacgatc ttccactcct tgcgcccgac cgtgcgcctg
     2281 aggatcgcgc gatcgccgag atgcagcgtg ggcttgaaga cccagaactc gtccgggtcg
     2341 atccgcccgc ccacgacggc ctcccccaag ccgtaggccg cgtcgatcac gacgacgtcg
     2401 cggaagccgc tctcgggatc gagcgtgaac atgaccccgg cggcgccgag gtcggcgcgg
     2461 accatcttct gcacggcgat cgacagccgg accgccatgt ggtcgaagcc gtgatggatg
     2521 cgataggaga tggcgcgatc ggtgaagagc gaggcgaagc agcggcggca cgcgccgagc
     2581 agcgccgccc ctccccgcac gcccaggtag gtctcctgct ggcccgcgaa gctcgcctcc
     2641 ggcaggtctt cggccgtcgc gctcgagcgc accgcgaccg caagcgtggg ctccgggccg
     2701 ccggcgagcg tacgataggc ctccgcgatc tgcgcctcga ggtccgcggg caccggggcg
     2761 cttctgatcc aggcccggat gtcggcgccg gcttcggcca gcgcgtcgag gtcccggccg
     2821 tccacaccgg tcagacgctt cgcgatacgg ctacggagcc cgccgacgtc cagcagcgcg
     2881 tcgtaggcgc cggcggtgat ggcgaagccg tcgggcacgc gaacgcctgc ggcccggagc
     2941 ctggtgtaga gctcaccgag tgacgccgtc tttccgccga cgaccgggac gtcggaggca
     3001 ccgatgtcgc ggaaccagcg aacgtaggac ggcgcgcccg gcggtggcga gcccatcacg
     3061 cgaagtacgc ctcgatctcg gcctgcagcc gccgggcggc ctcctcggac gtcggcgccg
     3121 tcgcctggaa gcggaggccg acctcgggag cgagcccgtt cttcacgacg ccgaccgcag
     3181 tccagccgag cccctgcacc tcgccgacct cgacctccac cagccggccc cggatacgat
     3241 cgctcgtcag cgtcatcacg aactccccac gtcagggctc gccgctcgag atcgctgcat
     3301 cggagatgcc agagccggtg gctcgattct gaacgtttag cgcgcgtggc gccggggtgg
     3361 gcgggcacgc gagccccggc tgggttatcg gtgccccacc ggcgcgcaac gcgccgtgtg
     3421 tccgcctcgt ggctcaggcg gcgctgccgc gaacggagag ctgggccgcg tggccccgcg
     3481 gtgtctcgca cgccgtggcg ctctcgcgca cgacgagcac cgggcgaccg gtgccgcgca
     3541 ggacggactc ggcgacgctg ccgaagcagt ctcgctgcag ccccgtccgt ccgtgggtgg
     3601 tcatcacgat caggtcgacc tggtactggt ccgccgcggt cacgatcgcc tggctcggcg
     3661 acccttgcca gagagcgacg gtgacgttcc gggcgccttc ctcccgcgcc cgccgggcca
     3721 cgtcatcgag gtaagtctcg gcctcacgcg tgacgtcgag ccgcgccgcc gcggactccg
     3781 ggcccggcag cggcgcctcg atcacgcgca gcagcagcag cgtggcgccg gcaccaccgg
     3841 cgaggtcgag ggcgaccggc agggcggcct cggccaccct cgagccgtcc agggaaacca
     3901 ggatgcgctt cgctttcatg ccgtcctccg cgccccgcac aggtgcaatt cattcgccag
     3961 tgtgccggcc acggccccgt gcgccgtgcc caccgtggcc tcagcccgcc aactcgggct
     4021 ccagccggac cggtgtgccg agcgacgcgc tgacgagaca cgcgcgctcc gccttctcga
     4081 gcgcgcgccg cacccggatg gagtcgacgc cggctggcag cgtcacgcgc ggccggagga
     4141 cgatctcggt gaaccgcgtc cggccgttcg agcggtcgac gatgccctcg ccgtcgacgg
     4201 acagcgacgt gaactcgacc cgcgaggcca ccgcgatggc ccggaacgtc agcaggaagc
     4261 aggcctcgac cgaggcgatc agcagctgct cgggactcca ggcgtcgccg gggccgccga
     4321 agtcgatcgg aggcgccgtg cgcaggtcgg gcacgcccgc gctcgacagc gtcgcgtagc
     4381 cctcgggacc accggcgatc ctggcttcgt agcgatgggg aagcggcttc atgacgtcct
     4441 cctggccctg aggtggacga accggtagac ggcgccgacg cgcagcgcgg cgcacggagc
     4501 cacacggcga tggggctcac gcatgcgacg cgcggctcgt cctcggctcg aggtactcga
     4561 cacaggcatc gagcgtggcg agcttcgggt agtccgcctc cgggatgtcg acgccgagcg
     4621 cgccgtgcag tgcgacgacg aaattgagga ggtccatcga gtcgaggtcg agctgctcgc
     4681 gaaaggccag gccgggactc agcgcggcga ggtcggcctc gggggcgatc tcgccaagca
     4741 cgtgaagcac ggtggcgcgc agcgcctcgc gcgtcacaac cgctccggcg cctgcagcca
     4801 gcgttccacc gcggccagga acaggccgcc gcggtggccg tcgctgaccc ggtggtcggc
     4861 cgacagcgtc gtggtgacga gcggccgcgg aacgatcgcg ccgtcgacga cccagggtcg
     4921 ctccacgatc ctaccgaagc cgacgatcgc gacctgcggc gggtaaacga tgccgaggac
     4981 tgtctcgacg ccctgctccc ccaggctcgt caccgtgatc gtcggatcgg acagctcgga
     5041 gctgcgcagc ccccccgtgc gcgcgcgctg caccaggtca cggaaggccc gcatgaggtc
     5101 gccgaggggg cggcggtcgg cgtcgtgcag cgccggcgcc acgagcccgc cgccgcggac
     5161 cgagatggca acgccgacgt ggacagccgc gctcggctcg gcgcgatcgc cgcgccacac
     5221 cgcgttcagc tccggcacct cgcgcagcgc gagggcgacg gccttgagca gcagcacgcc
     5281 gtagaggagc cggtcggtga cggggcgccg cgcgttctcc tcgccgagcc aggtcaggcc
     5341 gcggtgcatg tcgacggtgt gggcgaggta atagtggggg atctcccgct tggcgcgcgt
     5401 catcgcggca gcgatggtct ggcgcatgcg cgcggtgtcg aggcggggac gcccggccgc
     5461 cacggcgggc gcgggcgcgg cgggtcgcgc ggccgcgacc cgctcgacgt cctcgcgtgt
     5521 caccgtcccg cccggcccgg tgccgcgcag cgtcgccgga tcgatcccga gctgggccgc
     5581 gaccttgcgg gccagcggcg agatccgggc gcgctcgccg gccggcgccg gcccgtgcac
     5641 gacgggcgcc ggcgcgacgg ccgctggcgg agc
//
LOCUS       DATIAZ010000220         4741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1243817, whole genome shotgun sequence.
ACCESSION   DATIAZ010000220 DATIAZ010000000
VERSION     DATIAZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243817"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="VLF19_07745"
     CDS             <1..1962
                     /locus_tag="VLF19_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSE93184.1"
                     /translation="LAVLSVTAAILLRRSRWGLALVAIGGDEERAEVLGVRAIGVKIA
                     VFALSAALMGATGAAVAPRWTYIDPHIAFNPLISFQVIIMALLGGVGRPAGPVVGALF
                     LGLLSEFFLLQFRYVYMIVLGAVLILVVLGLPHGIMGWRAEERTEDLVRRLRWAIARR
                     IPRRYAMRQRYQLFVNGEWQDAPAGRTFPDVNPATGEVFTRIPAADAETARRAVEAAE
                     RARLEWGELQPAVRAGIVLEAAQIWERRRDDLEQMLTIETGAIRVKAGFEVGYCTELI
                     RQAAALPYQASGEVAPSNVSGKINYFVRKPVGVAAVISPWNFPYILTLRAVAPALALG
                     NAVVLKPSEETPLAGGLLVAEVFEEAGVPPGVLNVITCARADVEAVGDVMVTHPAVGV
                     VSFTGSTATGRALAEKAGRHLKRIVLELGGKSPLIVLEDADLDLAVSAAAFGGFFHQG
                     QICMATTRILVEHPLAEALASQLVEKLGTLKMGDLRDPQTAIGPITSPTQLEKIRAHV
                     EDAVAKGAKVLVGGHSRGSYFEPTLLTGVTPEMRCYYEETFGPVVTLTSVKNADEAVR
                     LANDTPYGLAAAIITGDPKRGMALAERIQSGIVHVNDSTVHDEPHAPFGGIKGSGLGR
                     HGGRAAIEAFTEIRWVSLQTERRHYPFDT"
     gene            2264..3289
                     /locus_tag="VLF19_07750"
     CDS             2264..3289
                     /locus_tag="VLF19_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSE93185.1"
                     /translation="MRSMFAGVLTALMLLPPVAPASAQGVGEVFRKVSPSVMVIRGRG
                     REVGAGGVTGFTETGSGVLISADGKVMTAAHVVHSLDEITVESLGGDTVTAKVIASEP
                     AADLSLIQLERVPRGAMVAKLADSNAVRVGDQVIVVGAPYGLGHSLSVGWISARWAPN
                     TVYKAMPLAEFFQTDAVINTGNSGGPMFSMAGEVIGLVSHNISKSGGSEGLGFVVTGN
                     TARQLLLEKRSIWAGLVGQILSDRQADILNLPPKRLGFIIQTVAKESPADALGLKGGT
                     AIATIDGQPLVVGGDIILSVAGVSVNSAADLVRIRETLARLPPGQPFEVTVLRAGQVL
                     ELTGRLP"
     gene            3341..3733
                     /locus_tag="VLF19_07755"
     CDS             3341..3733
                     /locus_tag="VLF19_07755"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSE93186.1"
                     /translation="MATIEKFCAPGVYDPPGYSQGMKVTGAQTILFLAGQVAYDKDGS
                     VAHRGDFKGQARLVFASLKALVESAGGTLANVVKLNTYVTDIRSRPEYRAVREEFFGA
                     KGPASTLVQVGALAHPDYLIEVEAIAII"
     gene            3831..>4741
                     /locus_tag="VLF19_07760"
     CDS             3831..>4741
                     /locus_tag="VLF19_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSE93187.1"
                     /translation="MTPEGFLRIEARVLELVAAGETLHAALDQLARAFEERATGMLVS
                     VLLVDGGGRLSHAAAPSLPEDYTRAIDTALGAGVASSGAAAFPARPDVAADIERDPFW
                     ADHRQLALRHGLRACWSTPILGLTRRVLGTFAVYYREPRSPTAEELALIDSGVHIARI
                     AIEHHHALEERDRLRAREQEAQNEAETVTAGKDLFLSTLSHELRTPLTAILGWTRLLR
                     IGAAPPSALPHALEVIERNTRLQERLISDLLDVSRIVAGTLRIETVPVDLVAVVEAAV
                     GSLLPAAGARGVTVERTLDAAAGPVLG"
BASE COUNT          683 a         1703 c         1650 g          705 t
ORIGIN      
        1 ctcgccgtcc tctccgtgac ggcggcgatc cttctacggc ggtcgcggtg gggactggcg
       61 ctcgtggcca tcggcggcga cgaggagcgg gccgaggtcc tcggcgtgcg ggccatcggg
      121 gtcaagatcg ccgtcttcgc gctctcggcg gcgctgatgg gcgccaccgg cgcggccgtc
      181 gcaccccgct ggacctacat cgatccgcac atcgccttca atccgctcat ctctttccag
      241 gtcatcatca tggccctgct gggcggggtc ggccggccgg ccggtcccgt ggtcggggcc
      301 ctgttcctgg gactcctctc cgagttcttc ctgctccagt tccgctacgt ctacatgatc
      361 gtcctcggcg ccgtcctcat cctcgtcgtc ctcggcctgc cccacggcat catgggctgg
      421 cgggcggagg agcggacgga ggacctcgtg cgacggctcc gctgggcgat cgctcggcgg
      481 atcccccgga ggtacgcgat gcggcaacgg tatcagctct tcgtcaacgg tgaatggcag
      541 gacgctccgg ccgggcggac gttcccggac gtgaaccccg ccaccggcga ggtgttcacg
      601 cgcatccccg cggccgacgc cgagacggcg cgccgcgctg tcgaggccgc ggagcgcgcg
      661 cgcctggagt ggggcgagct gcagcccgcc gtgcgcgccg gcatcgtgct cgaggccgcg
      721 cagatctggg agcgccgccg cgacgacctc gagcagatgc tgaccatcga gacgggcgcc
      781 atccgggtca aggcgggctt cgaggtcggg tactgcacgg agctgatccg acaggcggcg
      841 gcgctcccct atcaggccag cggcgaggtg gcgccctcga acgtgtccgg caagatcaac
      901 tacttcgtgc gcaagccggt gggagtcgcg gcggtcatct cgccgtggaa cttcccctac
      961 atcctcacgc tgcgcgcggt cgcgccggcg ctcgcgctcg gcaacgccgt cgtgctcaag
     1021 ccctcggagg agacgccgct cgccggcggc ctgctggtgg ccgaggtctt cgaggaggcc
     1081 ggagtcccgc ccggagtcct caacgtgatc acctgtgcgc gggccgacgt cgaggccgtg
     1141 ggcgacgtga tggtcacgca cccggccgtc ggcgtggtga gcttcaccgg ctccaccgcc
     1201 accggccgcg cgctcgccga gaaggccggc cgccacctca agcgcatcgt cctcgagctc
     1261 ggcggcaaga gcccgctcat cgtcctcgag gacgccgatc tcgacctggc cgtgagcgcc
     1321 gcggctttcg gcggcttctt ccaccagggg cagatctgca tggcgaccac ccggatcctc
     1381 gtggagcacc cgctggccga ggcgctggcc tcccagctcg tcgagaagct cggcacgctg
     1441 aagatgggcg acctgcggga tccccagacg gcgatcggcc ccatcacgag ccccacccag
     1501 ctcgagaaga tccgcgccca cgtggaggac gccgtggcca agggcgccaa ggtgctggtc
     1561 ggcggccaca gccgcggttc ctacttcgag ccgacgctgc tcaccggggt gacgccggag
     1621 atgcggtgtt actacgagga gaccttcggg ccggtggtga cgttgacgtc ggtcaagaac
     1681 gccgacgagg ccgtgcggct cgcgaacgat acgccctacg ggctcgccgc cgccatcatc
     1741 accggcgatc ccaagcgcgg catggcgctg gccgagcgga tccagtcggg catcgtgcac
     1801 gtgaacgact ccaccgtgca cgacgagcct cacgcgccct tcggcggcat caagggctct
     1861 ggcctgggcc gccacggcgg gcgcgccgcc atcgaggcct tcaccgagat ccgctgggtc
     1921 agcctccaga ccgagcgccg ccactacccg ttcgacactt gagtcgtgcc ttcatccggc
     1981 gcgccccagc gccggcgatg gtgcggcgcc tgtcttacaa tgcgcccagg agcacggccc
     2041 tggctcgacc gttggtcgag gaccgattcg ttcccaggag ggatcgatgc gcaacagccc
     2101 cgccgtcccc ccggcgccgc gagacccggc cggagccccg gatggctcag cgcgacgccg
     2161 gcgccgctcg tggagcggga tcgactgtcc ccggaggcaa cgcgcgaggc gatgatcgga
     2221 gcgatcgaga gttcacggag cgtcgatcac gaggaggtct gacatgcgat ctatgttcgc
     2281 cggggtgctg accgcgctca tgctcctgcc accagtcgcc cccgcctcgg ctcagggcgt
     2341 tggtgaagtc ttccgaaagg tgagcccgtc cgtgatggtg atccgtggcc gtggtcggga
     2401 ggtcggagca ggcggcgtga ccggcttcac cgagaccggt tccggcgtcc tcatctccgc
     2461 cgacggcaag gtcatgacgg cagcgcacgt ggtgcacagc ctggacgaga tcaccgtgga
     2521 gagtctgggt ggcgacaccg tcaccgccaa ggtcatcgcg tccgagcccg cagccgacct
     2581 gtccttgatc cagctcgagc gcgttccccg aggggccatg gtggcaaagc tcgccgactc
     2641 caacgccgtg cgagtcggcg accaggtcat cgtggtcggc gcgccgtacg ggctgggcca
     2701 ctcgctgagc gtgggctgga tcagcgcccg gtgggcgccg aacaccgtct acaaggcgat
     2761 gccgctcgcg gagttcttcc agaccgacgc ggtcatcaac acgggcaact cgggtgggcc
     2821 catgttcagc atggccggcg aggtcatcgg gctcgtgagc cacaacatct ccaagagcgg
     2881 tggcagcgag ggactcggct tcgtcgtcac cgggaacacg gcccgccagc tcctgctcga
     2941 gaagcgatcg atctgggccg gcctggtggg gcagatcctg tcggatcggc aggcggacat
     3001 cctcaacctc cctcccaagc ggctcggctt catcatccag accgtggcga aggagtcgcc
     3061 cgccgacgcg ctgggactca agggcggcac cgcgatcgcc acgatcgacg gtcagccgct
     3121 ggtcgtgggg ggcgacatca tcctgtccgt cgccggggtg agcgtcaaca gtgcggcgga
     3181 cctcgttaga atccgcgaga ccctcgcgag gctccctccc gggcaaccct tcgaggtgac
     3241 cgtgctcaga gccggccagg tgctcgagct gacgggacgg ttgccgtgac cgcgcagggc
     3301 gcgaatcctg atggcatgag caccgagaca aggaggacct atggcgacga tcgagaagtt
     3361 ctgcgctccc ggcgtctacg acccgcccgg ctacagccag ggcatgaagg tcaccggcgc
     3421 ccagacgatc ctcttcctgg ccgggcaggt ggcctacgac aaggacggct cggtcgccca
     3481 tcgcggtgac ttcaagggcc aggcccgtct ggtgttcgcc tccctcaagg ccctcgtcga
     3541 gtccgcgggc ggcaccctcg cgaacgtcgt caagctcaac acgtacgtga ccgacattcg
     3601 gagccgcccg gagtaccgcg ccgtccgcga ggagttcttc ggggccaagg ggcccgcctc
     3661 caccctggtg caggtcggcg cgctggcgca ccccgactat ctgatcgagg tggaggccat
     3721 cgcgatcatc tgaatgtgaa aatcttttcc ggggtggtcg agtacggctc cagcgggcac
     3781 aatcgtgccg cagaggctgc gcccatggac tcgagcggca gcggcggcgc gtgacgccgg
     3841 aggggttcct ccggatcgag gcgcgcgtgc tcgagctggt cgcggcgggc gagacgctgc
     3901 acgccgccct cgatcaactc gcgcgcgcgt tcgaggagcg ggccaccggc atgctcgttt
     3961 ccgtcctcct ggtcgacggg ggcggccgcc tgtctcacgc cgcggcgccg agcctgcccg
     4021 aggactacac gcgcgcgatc gacaccgccc tcggcgccgg cgtggcctcc tcgggtgccg
     4081 ccgcatttcc ggcgcggccc gacgtcgcgg ccgacatcga gcgcgacccc ttctgggcag
     4141 accaccggca gctcgctctc cgccacggcc tgcgggcgtg ctggtccacg ccgatcctcg
     4201 gcctgacgcg gcgcgtgctg ggcacgttcg ccgtgtacta ccgcgagccg cgctctccga
     4261 cggccgagga gctggcgctc atcgacagcg gcgtccacat cgcgcggatc gcgatcgagc
     4321 accatcacgc gctggaagag cgggaccggc tgcgcgcgcg cgagcaggag gcgcagaacg
     4381 aggccgagac cgtcaccgca ggcaaggatc tgttcctttc cacgctctca cacgagctgc
     4441 gcacgccgct gacggcgatc ctcggctgga cgcgtctgct ccggatcggc gcggcgccgc
     4501 cctcggcgct cccgcacgcc ctcgaggtca tcgagcgcaa cacccgactt caggagcgcc
     4561 tcatcagcga cctgctcgat gtctcgcgga tcgtcgcggg cacgctccgc atcgagacgg
     4621 tgccggtcga cctcgtcgcc gtcgtcgagg ccgccgtcgg gagcctgctg ccggccgccg
     4681 gggcccgcgg cgtcaccgtg gagcgcacgc tcgacgccgc cgcggggccc gtgctgggcg
     4741 a
//
LOCUS       DATIAZ010000221         3287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_324005, whole genome shotgun sequence.
ACCESSION   DATIAZ010000221 DATIAZ010000000
VERSION     DATIAZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324005"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VLF19_07765"
     CDS             complement(<1..586)
                     /locus_tag="VLF19_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSE93188.1"
                     /translation="MKDPAGAPHVLVVGSANVDFTVAAPRLPQPGETVTDGTLLVNHG
                     GKGANQALSARRLGADVRFIGCVGADDSGRAILRALAAEGVAVDGVAVTAAAATGTAL
                     IVVDLAGRNQIAVAPGANRALTIEQVSAREEDFVWADVVVASLEVPVPVAAWSLGRAR
                     RHRAIALLNPAPLPPTPLDFLGVVDYVTPNAGEAG"
     gene            complement(599..1528)
                     /locus_tag="VLF19_07770"
     CDS             complement(599..1528)
                     /locus_tag="VLF19_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HSE93189.1"
                     /translation="MERGSPAPVLIDTDPGIDDALALLLAWGSPEVRVEAVTTVAGNV
                     RVEDATLNVYRLIELRAPNPAPRVGRGAGGPLARRLRMATRYHGRDGLGDVPSWELSD
                     VRSVFDAGPEPGVPLLVETARRFGAALTLVALGPLTNVAIAVTSDRQAMGRVGRVVAM
                     GGAVDVPGNVTAEAEFNVHVDPEAAGVVLDAGLSLDLVPLDATRQATLMRRELEAALA
                     RCPGPVATRVAEFTRRAFRRHAGAMHLHDPLAVAVVVDPALVEWEAVRLEVDAGGATR
                     RVPGAPNCRIATRVDRARLLATLLPRLCAGQAR"
     gene            1653..1835
                     /locus_tag="VLF19_07775"
                     /pseudo
     CDS             1653..1835
                     /locus_tag="VLF19_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(1825..3090)
                     /locus_tag="VLF19_07780"
     CDS             complement(1825..3090)
                     /locus_tag="VLF19_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HSE93190.1"
                     /translation="MRAFEKVTLAGAVGRLAPRMKVLMPPGCGEPVALVAEICRQADR
                     LRDLTLIGGIHLGDYPFARPAAAALRYATWHMSPRLEDARRRGRVAFVPLRYFDLVTA
                     FAPGGAWAPDGVLVHCAPPDPHGYLSLGVAVGVALPAARRAPLVIAQVNPRMPRTRGS
                     GYLHRSQVDCWVEVDAPLYEYPPPAITEIERAIGRRVAALVPDGATVQVGVGAIPQAV
                     LEALHGHRDLALHSLLVDASVALVERGVVTCAAKPLHRGRMDVAEAMGTRRLFDFVHD
                     NPAVNMESSAWVHDPDVVARLDRFIAINSGLEVDLTGQVTAESLGARQVAGVGGQFDF
                     VLGASRSRGGASIVALPSTGRDGAASRIVARLAHGAAVTTPRFLVDRVVTEHGTAELR
                     GRDERERAAALIAVAHPRFREELERSLTS"
BASE COUNT          487 a         1220 c         1190 g          390 t
ORIGIN      
        1 gcccggcctc gccggcgttg ggcgtgacgt agtccaccac gcccaggaag tcgagcggcg
       61 tcggcggcaa cggcgcgggg ttcagcagcg cgatcgcccg atgccgccgc gcccgcccga
      121 gcgaccaggc cgccaccggc accggcacct cgagggaggc gacgacgacg tcggcccaga
      181 cgaagtcctc ctcgcgtgcg ctcacctgct cgatggtcag cgcgcggttg gcgccggggg
      241 cgaccgcgat ctggttgcgc cccgcgagat ccaccacgat gagcgcggtg cccgtggccg
      301 ccgccgccgt caccgccacc ccgtcgaccg cgacgccctc ggcggcgagc gcccggagaa
      361 tggcccggcc ggaatcgtcg gcgccgacgc agccgatgaa gcgcacgtcc gcgccgaggc
      421 ggcgcgcgga gagagcctgg ttggcgccct tgccgccgtg gttcacgaga agggtgccat
      481 cggtgacggt ctcgcccggc tgcggcaggc gcggcgccgc gacggtgaag tccacgttgg
      541 ccgagccgac gaccagaacg tgaggggccc cggcagggtc tttcataccc ctcctcgctc
      601 agcgggcctg tcctgcgcaa agccggggca agagcgtcgc gagcaggcgc gcgcggtcca
      661 cgcgcgtggc gatgcgacag ttcggcgcgc ccggtacccg ccgcgtggcg ccgccggcgt
      721 cgacctcgag gcgcacggcc tcccactcga cgagcgcggg gtcgacgacg acggccacgg
      781 caagcgggtc gtgcaggtgc atcgcgccag cgtggcgccg gaacgcccgc cgcgtgaact
      841 cggcgacgcg cgtcgccacc gggcctgggc accgcgccag cgcggcctcg agctcgcgac
      901 gcatgagggt cgcctggcgc gtggcatcga ggggcaccag gtcgaggctg aggccggcgt
      961 cgagcacgac acccgccgcc tcggggtcga cgtgcacatt gaactcggcc tccgccgtga
     1021 cgttgcccgg cacatccacg gccccaccca tggccacgac ccggccgacg cgtcccatgg
     1081 cctgccgatc ggaggtcacg gcgatggcca cgttcgtgag cggacccagc gcgacgaggg
     1141 tgagcgccgc gccgaaccgg cgcgcggtct cgacgaggag cggcacgccg ggctcgggcc
     1201 ccgcatcgaa gacggaacgc acgtcggaaa gctcccagct cgggacgtcg ccgaggccgt
     1261 cccggccatg atatcgggtg gccatccgaa gcctccgggc gagcggcccg cccgcgcccc
     1321 ggcccacgcg cggcgccgga ttcggcgcgc gcagctcgat caaccggtag acgttcagcg
     1381 tcgcgtcctc gaccctcacg ttgcccgcga ccgtggtgac cgcctcgacc cggacctccg
     1441 gcgaccccca cgcgagcagc agcgcgaggg cgtcgtcgat cccggggtcg gtgtcgatca
     1501 ggacgggcgc cggcgaacct ctctccacgg cacgggcatc ctaacgtatg attggaccag
     1561 gggcccggcc gaccgggacg cacgcctcac cgggaaggag gaacgggatg gagatccgga
     1621 tcaagtactg cggggagtga aactacctcc ctcaggcctc cagtctgcag gccgcgatca
     1681 agaagaagta cggtatcacc gccacgctcg acgaggggat gggcggcatc ttcgaggtct
     1741 tcatcgacgg caagagcgtc tactccaacc aggtgaccta ccgcttcccc gaggacgagg
     1801 agatcttcac caagatcgac gacgctagga cgtaaggctt cgctccagct cctctctgaa
     1861 ccgcgggtgg gccacggcga tcagggccgc ggcccgttct cgctcgtccc gtccccgcag
     1921 ctcggccgtg ccgtgctccg tcaccacccg atccacgagg aagcgtggcg tcgtgaccgc
     1981 tgcgccgtgg gccaggcgcg ccacgatgcg cgacgcggcg ccgtcgcgcc ccgtcgacgg
     2041 cagggccacg atcgaggcgc cgcctcgcga gcgcgaggcg ccgagcacga agtcgaactg
     2101 gccgccgacg cccgccacct gccgcgcgcc gaggctctcg gccgtgacct ggcccgtgag
     2161 atccacctcg agcccggagt tgatggcgat gaaccggtcc aggcgcgcga ccacgtccgg
     2221 gtcgtgcacc catgccgacg actccatgtt gaccgcggga ttgtcgtgga cgaagtcgaa
     2281 gagacgccgc gtgcccatcg cctcggcgac gtccatgcgg ccccggtgca gcggcttggc
     2341 ggcgcaggtc acgacgccac gctcgacgag cgccaccgag gcgtcgacga gcagggagtg
     2401 gagcgcgaga tcgcgatgac cgtggagcgc ctcgaggacg gcctggggaa tcgcgcccac
     2461 cccgacctgc acggtggcgc cgtcgggcac gagggccgcc acccgccgtc cgatggcgcg
     2521 ctcgatctcg gtgatcgccg gcggcgggta ctcgtagagc ggcgcgtcca cctccaccca
     2581 gcagtcgacc tggctccggt gcaggtagcc gctgcctcgc gttcgcggca tgcgcgggtt
     2641 cacctgcgcg atcacgagcg gcgcccgccg ggcagccggc agcgccacgc cgacggcgac
     2701 gccgaggctc aggtagccgt gcgggtccgg cggcgcgcag tgcacgagca cgccgtccgg
     2761 ggcccaggcg ccgccgggcg cgaacgcggt cacgaggtcg aagtaacgca acggcacgaa
     2821 cgccacgcgc ccgcggcgcc gggcgtcctc gagtcgcggc gacatgtgcc aggtggcgta
     2881 gcgcagcgcc gccgctgccg ggcgcgcgaa cggatagtcg ccgaggtgga tgccgccgat
     2941 cagcgtgagg tcgcgcagac ggtcggcctg gcggcagatc tcggcgacga gcgccacggg
     3001 ctcgccacaa ccgggcggca tcaacacctt catgcggggc gcgaggcgac cgacggcgcc
     3061 ggcgagggtg accttctcga acgcgcgcat ggggcggtta tatataccac gcaccggagt
     3121 cggccttcgt gcgagcggag gtttacaatg ggcggcatga tccgcgctcg tccgctcgcg
     3181 ctgctcccgt tgctgctgct ctcgctggcc ggctgcgcca cgtccacgac cactccgtcc
     3241 gagcggacgc gcgcggacac gacgcaggtg cgctgcctcg ccgcccc
//
LOCUS       DATIAZ010000222         4622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1639278, whole genome shotgun sequence.
ACCESSION   DATIAZ010000222 DATIAZ010000000
VERSION     DATIAZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639278"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VLF19_07785"
     CDS             <1..286
                     /locus_tag="VLF19_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSE93191.1"
                     /translation="AIAREVAEECGCVIHPVDIAGVVERIVRDDAGRIRYHYVLVDYL
                     AYADTDAVVAGSDADEARWVPLAEVERLETTDGLLDMIRRALALAERKEG"
     gene            297..1778
                     /locus_tag="VLF19_07790"
     CDS             297..1778
                     /locus_tag="VLF19_07790"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSE93192.1"
                     /translation="MDVRVLARSLVEVEADALVVGVPTDAGALPSRLAALDKRAGGQI
                     KAVLDAERFQGKVGQVTHVYTAGRLGVARVVVVGLGDGGDGTPESARRAAASGLRRAR
                     DLGARSVAIELLGDRLPARARAHAVVEGGILGAYAFDRYKKEKPEKSVERLVLVAPDA
                     RTARQAEEGARRGEVFARATWFARDLVNGPANEVHPSHLARVAQDVAREARLKLTLYD
                     RAACQKMGMGAFLGVAQGSQQPPRFIHLAYVPSGRVRRRVAVIGKGITFDAGGLDLKT
                     AEGMLRMKDDMSGAAAVLGIMRALGRLAPSVEVHGLVAATENMPSGTALRPGDVLRAM
                     NGTTIEVGNTDAEGRLTLADAICYANARVKPDEIIDMATLTGACVVALGPLCSGLFAS
                     EQGLADRLRIAAEQAGERLWQLPLIDEYREHLKSEVADINNVGPRGGSAITAALFLKE
                     FAGRTPWAHLDIAGSAFTEKDLPLAPRGGTGVAVRTILTYLTT"
     gene            1775..2608
                     /locus_tag="VLF19_07795"
     CDS             1775..2608
                     /locus_tag="VLF19_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSE93193.1"
                     /translation="MTAGRVDLHSHTTASDGTLSPRELVLEAVKRGVRVLAVTDHDST
                     EGLAEAREEALHHPPLEIVPGLEINCDVERAEIHVLGYFVDHQAAWFQAFLAEQRAER
                     VARVHRIAGRLAELGLPIDPAEVFAIVKEGSAGRPHVAQVMVRRGYVRSVREAFDRYL
                     RTGGPANVPRRRLSPVDAVTVIRRAGGVPVLAHPGLANRDELIPTLVEAGLMGIETYY
                     AEHSAAQTRDYLELCRRLDLVPTGGSDYHGPQSGRANPPGSPAVPWSSWEELKMRAGR
                     V"
     gene            2712..3071
                     /locus_tag="VLF19_07800"
     CDS             2712..3071
                     /locus_tag="VLF19_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HSE93194.1"
                     /translation="MTVQVFGFNDCQDTRKALRFFAERRVPAHFVDLARRPASKGELR
                     RFAEKFGAAALIDRESSRFLALGLRVSGDSPQRMLERAETEPRLLHTPLVRIGNRVSI
                     GHAPEAWQAALAAARPK"
     gene            3180..4025
                     /locus_tag="VLF19_07805"
     CDS             3180..4025
                     /locus_tag="VLF19_07805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSE93195.1"
                     /translation="MAVNPLSRREVLKFGAYTSVGGVLTPGISLAQAITTDTKGITAR
                     DVELTAGGAKIPGYDARPERGGRYPVVVVISGFTGNSEHNKDVVRRFAHAGYYAIAPE
                     LYHREGGMQGKSFPEMGQISGKVTRAQYLGDIRAAADFAKQQSWARADRLGVTGFCGG
                     GALTLHFSAEYPGVTAAVPWYGHVKRTYSDAPGVDAFALADRIKVPVLGLYGEADTGI
                     PVADVKAFEAALKSKGNQNVEFVLYPGAPHAFFSDDRPQVYKKEAAEDGWKRCLAFFD
                     KHLKA"
     gene            complement(4027..>4622)
                     /locus_tag="VLF19_07810"
     CDS             complement(4027..>4622)
                     /locus_tag="VLF19_07810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSE93196.1"
                     /translation="RLAETSETVLVEGRIAPVAAPVGTGRPPAALPADPLPVSTTCFA
                     CGVDNPIGLRLRLSFDDRAVAGRWQPGPSFRAANGAVAPVALTTMLDEAAFWLGALAS
                     GESGMTTDLSVTLRDPVPFGDVVTVAGCRDAVRQLADDPRYWATEVSAWDAAGRLVAD
                     ARITFVAVRGAARRLAGWLSRVNTSDVVRRVFPAYAR"
BASE COUNT          666 a         1652 c         1709 g          595 t
ORIGIN      
        1 ggccatcgct cgcgaggtcg ccgaagaatg cggctgcgtc atccatcccg tggacatcgc
       61 cggcgtcgtc gagcgcatcg tgcgcgacga cgccgggcgc atcaggtacc attacgtgct
      121 cgtggactat ctcgcctacg cggacaccga cgccgtcgtc gccggcagcg acgccgacga
      181 ggcgcggtgg gtgccgctcg ccgaggtcga gcgcctcgag acgaccgacg gcctcctcga
      241 catgatccgc cgcgcgctcg cgctggcgga gcgaaaggag gggtgagcga cccgtcatgg
      301 acgttcgcgt gctcgcccgg tccctcgtcg aggtcgaggc cgacgcgctc gtcgtgggcg
      361 ttcccaccga cgccggggcg ttgccgtcgc gcctcgccgc gctcgacaag cgcgcgggcg
      421 gacagatcaa ggccgtgctc gacgccgagc gcttccaggg caaggtcggg caggtcacgc
      481 acgtgtacac ggccggccgc ctcggcgtcg cgcgcgtggt ggtggtcgga ctgggcgacg
      541 gcggtgacgg cacgcccgag agcgcgaggc gcgcggcggc cagcggattg cgccgggccc
      601 gggatctcgg cgcccggtcg gtggccatcg agctgctcgg cgaccggttg cccgcgcggg
      661 cacgcgctca cgctgtggtc gagggaggca tcctcggggc gtacgcgttc gaccgctaca
      721 agaaggagaa gcccgagaag tcggtcgagc ggctcgtgct cgtcgccccc gacgcgcgga
      781 cggcccgcca ggccgaggag ggcgcgcgtc gcggcgaggt gttcgcgcgc gcgacgtggt
      841 tcgcgcgcga cctggtcaac gggccggcca acgaagtgca cccctcgcac ctggcacgtg
      901 tggcgcagga cgtcgccagg gaggcgcggc tcaagctcac cctgtacgac cgcgccgcgt
      961 gccagaagat gggcatgggc gccttcctcg gcgtcgccca gggcagccag cagccaccgc
     1021 ggttcatcca cctcgcctac gttccgtcgg gacgggtgcg ccggcgcgtg gcggtgatcg
     1081 gcaagggcat cacgttcgac gccggcggtc tcgatctcaa gaccgccgag ggcatgctgc
     1141 ggatgaagga cgacatgtcg ggcgccgccg ccgtgctcgg gatcatgcgg gcgctcggtc
     1201 ggctcgcgcc gtccgtcgag gtgcacgggc tcgtggcggc caccgagaac atgccgtcgg
     1261 gcacggcgct ccggcccggg gacgtgctgc gcgcgatgaa cggcaccacg atcgaggtcg
     1321 gcaacaccga cgccgagggc cggctcacgc tcgccgacgc gatctgctac gccaacgctc
     1381 gcgtgaagcc cgacgagatc atcgacatgg cgacgctgac gggggcctgc gtcgtcgccc
     1441 tcggtccgct ctgctccggg ctcttcgcca gcgagcaggg gctcgccgac cggctgcgga
     1501 tcgccgccga gcaggcgggc gagcgcctct ggcagctgcc gctgatcgac gagtaccgcg
     1561 agcatctcaa gagcgaggtc gccgacatca acaacgtggg gccgcgcggc ggcagcgcca
     1621 tcaccgccgc gctcttcctc aaggagttcg ccggccggac gccgtgggcg cacctcgaca
     1681 tcgccggctc ggcgttcacc gagaaggacc tgccgctcgc gccccggggc ggcaccggcg
     1741 tggccgtgcg gacgatcctg acgtacttga ccacgtgacg gcggggcggg tcgacctcca
     1801 cagccacacg accgcgtccg acggcacgct gtcgccgcgg gagctcgtgc tcgaggccgt
     1861 gaagcgcggc gtgcgcgtgc tggccgtcac ggaccacgac tccacggagg ggctcgccga
     1921 ggcgagggag gaggcgctcc atcacccgcc gctcgagatc gtgcccgggc tcgagatcaa
     1981 ctgtgacgtc gagagggccg agatccacgt gctcggatac ttcgtggacc accaggccgc
     2041 ctggttccag gcgttcctcg ccgagcagcg ggccgagcgc gtggcgcgtg tgcaccggat
     2101 cgccgggcgc ctggccgagc tgggcctgcc catcgaccct gcggaggtct tcgcgatcgt
     2161 gaaggaagga tccgcggggc ggccccacgt cgcgcaggtg atggtgaggc gcggctacgt
     2221 caggtcggta cgcgaggcgt tcgaccggta tctcaggacc ggagggcccg cgaacgtccc
     2281 gcgccgccgg ctgtcacccg tcgacgccgt cacggtgatc cgccgggcgg gcggtgtccc
     2341 cgtgctggcg catcccgggc tggcgaaccg ggacgagctg atccccacgc tggtcgaggc
     2401 ggggctcatg gggatcgaga cgtactacgc cgagcactcc gcggcgcaga cccgggacta
     2461 tctggagctg tgccggcgcc tggacctcgt gccgacgggc ggctccgact accacggacc
     2521 gcagagcggg cgggcgaacc cgcccggcag cccggccgtg ccctggtcga gctgggagga
     2581 attgaagatg cgggccgggc gtgtatgagg gatcctcgcg ccgaccgcct cgcgcgagct
     2641 cgccgcggcc gccgcgcgcc cggcggcagc gcgcgccgat cgagcggacc ggtcacgcgg
     2701 ccctcgcgac gatgaccgtg caggtcttcg gcttcaacga ctgccaggac acgcgcaagg
     2761 cgctgcgctt cttcgccgag cgccgcgtcc cggcccactt cgtggacctg gcgcggcggc
     2821 cggcctcgaa gggcgagctc cggcgcttcg ccgagaagtt cggcgcggcc gcgctgatcg
     2881 accgcgagag ctcgcgcttc ctggcgctcg gcctgcgcgt cagcggtgac tcgccgcagc
     2941 ggatgctcga gcgcgccgag accgagccgc gtcttctcca caccccgctc gtccgcatcg
     3001 gcaaccgtgt cagcatcggc cacgcgccag aggcctggca ggcggccctc gcggcggccc
     3061 ggccgaagtg agtcgggcgg ggcaggaacc ggagccgggt cccggcggtt agtgaggcgg
     3121 cagggccgcg gagccttgtc tgcgttcggt ctcccacccg gttccgacag gaggcattca
     3181 tggccgtcaa tccactgagc cgaagggaag tgctgaagtt cggtgcctac acgagcgtcg
     3241 gcggcgtgct gacgcccggc atctcgctgg cccaggccat cacgaccgat accaagggca
     3301 tcaccgcccg ggacgtggag ctgaccgccg gtggtgcgaa gattcccgga tacgacgccc
     3361 ggcccgagcg cggtggtcgc tacccggtcg tcgtcgtgat ctccgggttc accggcaaca
     3421 gcgagcacaa caaggacgtc gtccggcgat tcgcccacgc cggctactac gcgatcgcgc
     3481 ccgagctcta tcaccgcgag ggcggcatgc agggcaagag cttccccgag atgggccaga
     3541 tctccggcaa ggtcacgcgc gcgcagtacc tcggcgacat ccgcgcggcg gcggacttcg
     3601 ccaagcagca gtcgtgggcc cgcgccgatc gtctcggtgt caccggattc tgcggtgggg
     3661 gggccctgac gctgcacttc agcgcggaat acccgggcgt cacggcggcg gtgccgtggt
     3721 acggacacgt caagcggacg tactccgacg cgcccggcgt ggacgccttc gcgctcgccg
     3781 acaggatcaa ggtcccggtg ctcgggctct acggcgaggc ggacacgggc atccccgtgg
     3841 ccgacgtcaa ggctttcgag gccgcgctca agagcaaggg taaccagaac gtcgagttcg
     3901 tcctctatcc gggcgcgccc cacgccttct tctcggacga ccggccgcag gtctacaaga
     3961 aggaagcggc cgaggacggc tggaagcgct gcctcgcctt cttcgacaag cacctgaagg
     4021 cctgagtcac ctcgcgtagg cggggaagac gcggcggacg acgtcggacg tgttcacgcg
     4081 cgagagccac ccggcgagcc gccgcgccgc tccccgcacc gcgacgaagg tgatgcgcgc
     4141 gtcggcaacg aggcggccgg cggcgtccca ggccgacacc tcggtcgccc agtagcgcgg
     4201 atcgtccgcg agctggcgga cggcgtcgcg gcagccggcg acggtcacga cgtcgccgaa
     4261 cggcaccggg tcgcgcaggg tcacggacag gtccgtggtc attcccgact ccccgctcgc
     4321 cagcgccccc agccagaacg ccgcctcgtc gagcatcgtg gtgagcgcca ccggcgcgac
     4381 cgccccgttg gccgcgcgga acgacgggcc cggctgccac cggccggcga cggcccggtc
     4441 gtcgaacgag agccgaagcc tgagcccgat cgggttgtcc acgccgcacg cgaagcacgt
     4501 cgtggacacg ggcaggggat ctgcgggcag cgcggccgga ggccggccgg tgcccacggg
     4561 agcggcaacg ggagcgatcc gcccttcgac gagcacggtc tcggaggtct ccgcgagccg
     4621 ac
//
LOCUS       DATIAZ010000223        19569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_850623, whole genome shotgun sequence.
ACCESSION   DATIAZ010000223 DATIAZ010000000
VERSION     DATIAZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 19569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19569
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850623"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..743
                     /locus_tag="VLF19_07815"
     CDS             261..743
                     /locus_tag="VLF19_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSE93197.1"
                     /translation="MAKQVLSLTVNDEAVEVLVQPYDTLLDSLREGLGLTGPKEGCGT
                     GDCGACTVHLDGKPVASCLVLAMQARGRSVKTIEGLGRHDALHPLQDAFVRHGVPQCG
                     FCIPGVLMAAAALLDENPRPTEEEIRYGIAGNLCRCTGYTKMLAAISEAAGTAAGARS
                     "
     gene            747..3068
                     /locus_tag="VLF19_07820"
     CDS             747..3068
                     /locus_tag="VLF19_07820"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE93198.1"
                     /translation="MATVMKTTRDVKGVGLSIPRPDGPEKVTGQVQYVADIKPRGLLH
                     AKLLRSPHAHARIVRIDASRAKALPGVRAVLTAADVPELKRKAPTRAHAVLAIDRVVF
                     VGQPVAAVAADEPAIAEEALDLIDVQYEVLPAAVDPLESMKPGAPPVADVGTEADTSE
                     AQAHGSVAGIAAEAKPAKAVNISQQARIQRGDVAKGLAASDVVLEKTYRVPMVHQGYL
                     EPHAVLAQWDTTGHLTLWASTQGSFNTRSEVADVLELPENSITVIPVECGGGFGGKIR
                     ALCEPITALLARAAGRPVRYVMTRREELEAGMPAPQVIIRLKTGVKRDGTLMALEGEA
                     VIESGAFSGAVLTVSGVFLASLYRWPAFDVRGFEVLTHKPSIAAYRAPVAPHTIFALD
                     SHMEQLARAVGLDPVEFRLRHLQREGDPMANNQPWLSNGAVEVLQRTTEHPIWKSRAE
                     WKASARDGRLRGTGLALGGWLGGLQPTGATVRLNPDGSLQVLTGQVDIAGTNIALAQI
                     AATAYGVDVDRVRITTGDTDVAPVTGLSAGSKTVYTVGVAVMEAARDARRQTMEIAAK
                     ELEASVHDLEIEDGRVVVRGVPDRGITLAQIGKKGNLYMSKVPPVLGKSHPAFSQQAP
                     AFCAELARIEVDPDTGEVTIHDFVVVQDVGKAINPLGVEGQMQGGAVQSLGMALTEGL
                     MFDAAGRLTNPSLLDYRKLTAADLPDIETIIVEKPSPAGPFGARGVGEPPIVPAPAAI
                     ANAIEDATGVRLTELPLTPERIALGIAARRALG"
     gene            complement(3156..3509)
                     /locus_tag="VLF19_07825"
     CDS             complement(3156..3509)
                     /locus_tag="VLF19_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSE93199.1"
                     /translation="MKPKLRVWVVFGQRLKFGDGRARLLELIGEHGSLRKAAAELGMS
                     YRNAWGYLRELEKAAGFKLVERAPEGTSRSGMRLTSTGRDFLSCYRKFQRSLDGAATR
                     HFDRAFGGKVFRSRG"
     gene            3823..4653
                     /locus_tag="VLF19_07830"
     CDS             3823..4653
                     /locus_tag="VLF19_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSE93200.1"
                     /translation="MARLLLAAAVVLALVHPGEAQAPSTTVILSTTTSTQDSGLLDVL
                     VPLFERKTGHSVKTISVGTGQALALAARGEADVTLAHAPSLEKKYVAEGKMSNRRLVM
                     YNDFVIIGPEDDPAKIKGGKCSLDALEKIAAAGSRFVSRGDKSGTHNLELALWKQAGV
                     TPAAPWYIESGQGMGATLGIADDRRAYTITDRATLLAFSKRLRLQTMVEGDRPLLNIY
                     SVMEVNPANGPRVNAAGGKAFADFMLAPETQAVIKTFGVDKYSQPLFVPIAGKKDEDF
                     "
     gene            4753..5445
                     /locus_tag="VLF19_07835"
     CDS             4753..5445
                     /locus_tag="VLF19_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93201.1"
                     /translation="MDLVLAGVRQAIALLFAGDAEVWAILLLSLQVSGTATLLALGIG
                     VPAGTALALTRFPGRTLLVGAVNTGMGLPPVVVGLFVTLLLWRSGPFGDWEVLYTPAA
                     IVLAQAVIASPIVTGITLAAVQNVPEPFRLQLLGLGASRLQMVSVVLREARLPMLAAV
                     MAGFGGIISEIGASMMVGGNIKGQTRTLTTAMVLETGKGNFDIAIALSILLLTLVFLV
                     NWLLTVLQQRAH"
     gene            5518..6288
                     /gene="modA"
                     /locus_tag="VLF19_07840"
     CDS             5518..6288
                     /gene="modA"
                     /locus_tag="VLF19_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSE93202.1"
                     /translation="MSRLLLALAVCSLAGAVPAAAQEVTVSVAISMKEAIEDIGRRFS
                     AGQPGLALRYNFGASGELQKQIEGGAPVDVFVSAAQRQVDELDRAGLLLAGTRVVFAR
                     NVLVALKPRDGVLDVPGPSDLLDRRVQRIAIGSPKTVPAGQYAEESLRALGLWDRLKH
                     KLVHGENVRQVLDYVVRGEVDIGVVYATDGALRLDHVRLAFAFSEDTHRPIVYPAAVV
                     AATRQPVLAKAFVEFLTGREAQAVLGRLGFEPAPAGAR"
     gene            6285..6950
                     /gene="modB"
                     /locus_tag="VLF19_07845"
     CDS             6285..6950
                     /gene="modB"
                     /locus_tag="VLF19_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HSE93203.1"
                     /translation="MSPEALSALALSVRVATVATLLNAVIGIPLAYVLARRAFRGRTL
                     LDLLVTLPLVLPPTVTGYYLIVLLGRRGWLGGPLYELTGWTVAFTWYAAVVAATVMAL
                     PLLVRTARAAIESVDRELERAAWTLGRPEWRTALEVTLPLARNGIVAGLVLAFARALG
                     EFGATLMLAGNIPGRTTTVPLAIYTAVQTGERGDALVLVAALTALSCVVLVLAGRLGA
                     RAA"
     gene            6958..8082
                     /locus_tag="VLF19_07850"
     CDS             6958..8082
                     /locus_tag="VLF19_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93204.1"
                     /translation="MARLEVALVKRLPGFALDAAWSAGDGVAVLFGPSGAGKTLTLQC
                     LAGLLRPDAGRIVVDGTVYFDAAAGIDLPAQRRRVGYVFQGYALFPHLTVAENVGFGL
                     RNRPRAERVERTRAVVERLGLGGLEGRRPAELSGGQRQRVALGRALAIDPVLLLLDEP
                     LSALDAPLRRVLRDELRGVLAESGVAAVVVTHDFAEAYRLADRVVVYEGGRVIQAAPR
                     SELLWQPASEAVARIMGIRNILRGTVVKATPDRIQLRWRGQLLEAVNSPTRAYLPPPD
                     SPIAFFIRPEYVRLVRKDRGAPDAQHHMNLMAGEVVGEADFGTTWTLTIRLDEPGAPA
                     QGEHDLEVEVPRLVYEILEIERDRRWQLSIHRGSIQVLPA"
     gene            8123..9199
                     /locus_tag="VLF19_07855"
     CDS             8123..9199
                     /locus_tag="VLF19_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93205.1"
                     /translation="MLALRDVVVGYSGAFSVSVPVLEVAAGEVLALIGPNGSGKSTLL
                     RVLALLQAPDEGTVAFRGRAVSVADGLDVRRRMAVVFQQPLLADMRVEDNVELGLRFR
                     GVPPRERRTRVDRWLERLQIGALAGRPARTLSGGEAQRVALARALAPEPDVLLLDEPF
                     GALDAPTRATLVPDLAAILRSDRVTTVIVTHDRGEAQALADRVGVMFGGRLHQVDETA
                     RLFGAPASEEVARFVGVETILAGEVVDAEDGVAVVEVGGRRVEVAAIAQRGERLRVGI
                     RPEDVTLSTGAEPGASSARNHFVGTVVRLTAGSPVRVVLDCGFPLVAAITARSAAELR
                     LAAGARVTAVFKASAAHVIRAGSS"
     gene            9371..9592
                     /locus_tag="VLF19_07860"
     CDS             9371..9592
                     /locus_tag="VLF19_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HSE93206.1"
                     /translation="MNQESLIERLTAENEEFRRLRTEHQDYDRELETLKGDSTLSAQQ
                     QWRMTELKKLKLMAKDRMENIIRHTRVTA"
     gene            9619..10122
                     /gene="moaC"
                     /locus_tag="VLF19_07865"
     CDS             9619..10122
                     /gene="moaC"
                     /locus_tag="VLF19_07865"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSE93207.1"
                     /translation="MPRNAATALRGLTHLTPAGEARMVDVSAKAETAREAVARATLRM
                     SPRTLRVVRAGNAPKGDVLGVARTAGILAAKRTHELIPMCHPLRVTGVDLAFSDDVRR
                     GELTIEARVRTVDKTGVEMEALTAASVAALTVYDMVKAVEKGVTIERVQLVEKSGGKS
                     GTWKRRT"
     gene            10119..11249
                     /locus_tag="VLF19_07870"
     CDS             10119..11249
                     /locus_tag="VLF19_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HSE93208.1"
                     /translation="MKTALIYSDEWTRFDYGPEHPLRMERLGLTWKLMEAYGLTSLPT
                     GAVTAPDPATEAELLKFHSEEYLTVLKAANSGVAPAAVICHYGLGPGDNPVFPGLWEA
                     ARLVAGGSLLGARLVAGGEVQRAFHFAGGLHHAMTDRASGFCYVNDAVLAILHLRTQG
                     LRVAYVDIDAHHGDGVQAAFYGERDVLTISTHERGDRLFPGTGFVEEIGEGSGRGFSV
                     NLPLDVLTDASVYLPAFERVVPSLLKAFRPDVIVAQLGIDSHRTDPLTHLALDVQGFA
                     RAVARIAEFAPRLLALGGGGYDLGNVARAWTAAWAVMNGVELPAEIPAGFRDYARWLR
                     VRPASLWDPPAVIDDERRRLAREYVDRQVDKIERLIFPLHGL"
     gene            11324..12328
                     /locus_tag="VLF19_07875"
     CDS             11324..12328
                     /locus_tag="VLF19_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE93209.1"
                     /translation="MRRFRRGLGRAPVVLPPRDTAWRSIAPGFEEAREMAASGLPVQI
                     AAARRYDRAPDRRRIASALVRGETVFMPQVHQVLPRVARLMVAIRVILMGPFREECSF
                     LFLVGGGGREGMGLHHDGPVDSFWLQLAGRRTVTIGPRVEPGSPEDIEAPGWGRGWQT
                     FDLPPGTLFYLSPWTPHRVVCRVRSLALSLTWSPPRRAATAGGVRSRPATARARTAAV
                     RARAESLSRWDVVDGRVTARPGRSSGRLWTQVPAIAGAPDARGRFRLLTPDGALVLDR
                     AARPLAGRLADMPALERAAVDGRAALAPLLAHGILGDEDLPRRIVPADPEGLDGWRFA
                     "
     gene            12545..13159
                     /gene="tmk"
                     /locus_tag="VLF19_07880"
     CDS             12545..13159
                     /gene="tmk"
                     /locus_tag="VLF19_07880"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSE93210.1"
                     /translation="MRGVLITSEGVEGSGKTTQMARLGRWLEKQGYRVECTREPDGTP
                     LGAAIRRLFERPGVRPLTEVFLFMAARQQHVAERIAPWLKRGAVVLCDRYTDATVAYQ
                     GYGRGIDRDLIRELNVRATGGILPDLTLLFDLDPREGFRRIGRRRLDRFEREALAFHR
                     RVRRGYLEIQRAEPKRVRLVRAGRPPAVVATEVEAMVGEFFRGT"
     gene            13149..14033
                     /locus_tag="VLF19_07885"
     CDS             13149..14033
                     /locus_tag="VLF19_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93211.1"
                     /translation="MALEAIRDQPRVVDLLRRALASGRVAHAYAFLGAPGSGRTTAAR
                     AFGAALLCEHGGCGACRGCTLAAAGRHPDLHVIVPTPPEKNPRGPLAVRIDDIRALEH
                     AAALAPVMAPRKVFVLEDADRMTGETPQAFLKTLEEPPPRTVMILVLQRARAVPATVL
                     SRCQIVRFAPPPAAAAPERAEALEALLEVRGKGADALFRRSQVFDRDRERAERFVDGC
                     WLLCRDLLLAKAGAPPRLLVRPEQAEVLAREAGDWSQDALLASIAACREARAALAVNV
                     TPRLTLEIVLGRLASRAA"
     gene            14041..14928
                     /gene="ricT"
                     /locus_tag="VLF19_07890"
     CDS             14041..14928
                     /gene="ricT"
                     /locus_tag="VLF19_07890"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HSE93212.1"
                     /translation="MSDQALNDTAVAEYLVGVRLREVAQTEDHRLAGDDLIPHVGDLV
                     IVDTGSGEAVAEVRRPRRAIPEAKRGRGWPRIVRAANETEAREWRERLEREKKAVVTS
                     QRLARAAGLVMKIVDVEMHPAARRVTVYFNADERVDFRQLVRDLAREFRSRIEMRQIG
                     ARDTTKAMDGIGPCGRQLCCSSYLRKFEPISVKMAKAQDMPLTDSRLLGNCGRLKCCL
                     LYEFSLYQELRARLPKVNTPCQASCGGGGCMSGKIKSLRVLKQSVVVGFPDGTEAEVP
                     LDQVTWEGRPHVQAQLDKT"
     gene            14939..16897
                     /gene="metG"
                     /locus_tag="VLF19_07895"
     CDS             14939..16897
                     /gene="metG"
                     /locus_tag="VLF19_07895"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSE93213.1"
                     /translation="MSTFYLTTPIYYVNAPPHLGHAYTMVVGDALTRWHRMAGDDVLF
                     LTGTDEHGDNIAQAAARAGVTPQALADQNSAAFRRAWDELGIRYDDYIRTTEPRHKVV
                     VQAILQRLWDAGEIYLGRYGGHYCFGCERFYTEKEIVDGQCPDHQRPLTWVEEENYFF
                     KMSKYQDWLVGYIHDHPDLIRPERYRNEILGFLRDPLQDLSISRPKKRLQWGIPLPFD
                     DDYVTYVWFDALINYVSALGGPGAPKFDSYWPHARHLIAKDILKPHAVYWPCMLNAAG
                     LEVYRQLNVHGYWTVGGRKMSKSVGNLVDALAVKDRFGNDAFRYFVMREMTFGLDADF
                     SEEPFIGRLNADLANDLGNLVSRATTLIVNGGGISRVPEARELGELEADVLGGFDKAR
                     QELDAAMADFAFQRALAAIWGWIGRLNGYVDATQPWALAKQPAQRARLEVVLYTLAES
                     LRLLGIVLAPFLPDAASKIRAALGETGELRLADATWSRLAAGARIQKLSGLFPRVDAA
                     RGVEAPGAAGEVGVGPATEAAGRAGPVTTTPVVAPERSIVRITDFQKIDLRVAEVVGA
                     EPVPKSKKLVKLTVSLGDERRTVVAGIAEHYAPADLVGKKVVVVANLEPATLMGVESQ
                     GMLLAGSAEGALAVLTLDRDLPPGAKVR"
     gene            16902..17132
                     /locus_tag="VLF19_07900"
     CDS             16902..17132
                     /locus_tag="VLF19_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93214.1"
                     /translation="MTDVDVMIQVAGQEPSIARVLREICALEGGVRSLALDLVAAQLR
                     THARSRDLLEAIAALRRDDVARRIAEALARTR"
     gene            17129..18193
                     /locus_tag="VLF19_07905"
     CDS             17129..18193
                     /locus_tag="VLF19_07905"
                     /inference="COORDINATES: protein motif:HMM:NF039296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HSE93215.1"
                     /translation="MSVETEPQALAQPAVEAGALVRRGRNNPLVPFFRRWGGSIASSV
                     GGLLTLLVFRHGVAHVRWIVGYLLLAWIISAMVVQIHRAIVAAERRVHRLVIAAADYA
                     IQSLHHGVLLFLLPPYWASVTLTSVNAPFFVLLVGFVVVATVDPWYRALVQPRPWLTA
                     LFFFVSTLGALNLALPLVGVPPHTALALAAYVATVALTPLLVHARGWPWLHAVGGTAV
                     AGVALAALVYAGRAGVPPAPLFVAEKRLAWNFAMADYLQPVARAIPTRELRERGLVVH
                     TAVYAPAGLTQPIRHVWRQHGKVVDDVTLSPVRGGRFEGFRTFSRKTAWPADPRGKWT
                     VDVVTGAGQLLGRLSFRVTG"
     gene            18190..18966
                     /locus_tag="VLF19_07910"
     CDS             18190..18966
                     /locus_tag="VLF19_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSE93216.1"
                     /translation="MTPDLFDTHAHLHFPDFAPDLPAVLERARAAGVRRCLTIGTDVE
                     SSRAAVALAAREPDVWASVGIHPHDAAAADDAALAEIARLARAPRVVAVGEIGLDWFR
                     DLSPRPAQEAAFRRQLALAREIGRPVLVHCREAHADVLRILGEEHAVDRGGIMHCFSG
                     DVAIARRCLELGFLVSLAGPVTYPKARALPEVARWTPGDRLVIETDCPYLPPQAHRGQ
                     RNEPAYLALTAARVAELRGEPLPELAARLTANACAMLGVP"
     gene            18963..>19569
                     /locus_tag="VLF19_07915"
     CDS             18963..>19569
                     /locus_tag="VLF19_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSE93217.1"
                     /translation="MSLRDVGFYVRGRVSSGVARALNRRRAVTLPPPVPLTEVLRGPG
                     SSSALVRLLEGRANHEREAAVAAWLAARGVPFARHRFATFEGRGENLGVEIGSGDRVL
                     VLIAHHDAVPGSPGANDNAAAVAILLGLLPRLERVVPPGLRVRLLFMACEELGYLGAR
                     AYVRDTPLGSIVGVLSLELCGIGDSLALWDAVEETPFRRRVT"
BASE COUNT         2573 a         7093 c         7079 g         2824 t
ORIGIN      
        1 cgacccggcg tagccgtctc gcgcctcggt ctctcgtgcg atggtcgacg gacatggggc
       61 cccgagatgg cccccgagcc ccccgggcgg ctcggtcgcg acccggcgta gccgtctcgc
      121 gcctcggtct ctcgtgcgat ggtcgacgga catggggccc cgagatggcc cccgagcccc
      181 cagcgttcgg ccgctccggc gcagccgtgg cgcgcctctg ggacccgctg agtttcctga
      241 actaggcaag gagtcgccac gtggccaagc aggtactgag cctcacggtc aacgacgaag
      301 cggtcgaggt gctcgtgcag ccctacgaca cgctgctgga ctcgctgcgc gagggccttg
      361 gcctgacggg accgaaggaa ggctgcggca ccggggattg cggggcctgc accgtccatc
      421 tcgacggcaa gccggtcgcg agctgcctcg tgctggccat gcaggctcgt ggccgttcgg
      481 tgaagacgat cgaagggctc gggcggcacg acgccctcca cccgctgcag gacgcgttcg
      541 tgcgtcacgg cgtgccccag tgcgggttct gcatcccggg ggtgctcatg gccgcggcgg
      601 cgttgctcga cgagaatccg cggccgaccg aagaggagat ccgctacggc atcgccggca
      661 acctctgccg gtgcacgggc tacaccaaga tgctggccgc tatcagcgag gctgcaggca
      721 ccgcggccgg tgcgaggagc tgagacatgg cgaccgtgat gaaaaccacg cgtgacgtca
      781 aaggcgtcgg cctctcgatc ccgcgtcccg acgggccgga gaaggtcacg ggccaggtcc
      841 agtacgtcgc cgacatcaag ccgcgcgggc tgctgcacgc caagctcctg cgcagccccc
      901 acgcccacgc ccgcatcgtg cgcatcgacg cgagccgggc gaaggcgctg cccggtgtcc
      961 gcgccgtgct gacggccgcc gacgtccccg agctgaagcg caaggcgccg acccgcgccc
     1021 atgcggtgct ggccatcgac cgcgtggtct tcgtggggca gccggtggcg gccgtcgccg
     1081 ccgacgagcc ggcgattgcc gaggaggccc tcgacctgat cgacgtccag tacgaggtgc
     1141 tcccggccgc cgtcgatccg ctcgagtcga tgaagcccgg cgcaccgccg gtcgccgacg
     1201 tcgggaccga ggctgacacc agcgaggcgc aggcccacgg cagcgtggcc ggcatcgcgg
     1261 ccgaggccaa gcccgccaag gccgtcaaca tctcgcagca ggcccgcatc cagcgcggcg
     1321 acgtggcgaa gggcttggct gcgtccgacg tggtgctcga gaagacctac cgcgtgccga
     1381 tggtccacca gggctatctc gagccgcacg cggtcctggc ccagtgggac accacggggc
     1441 acctgacgct gtgggcgagc acgcagggct cgttcaacac acgctcggaa gtggccgacg
     1501 tgctggagct gccggagaac agcatcacgg tcatccccgt ggaatgtggg gggggcttcg
     1561 gcggcaagat ccgtgcgctg tgcgagccga tcaccgcact gctcgcgcgc gccgccgggc
     1621 ggcccgtgcg ctacgtgatg acccggcgcg aggagctcga ggcggggatg ccggctcccc
     1681 aggtcatcat ccgtctcaag accggtgtga agcgagacgg cacgctgatg gcgctggagg
     1741 gggaggcggt catcgagtcc ggcgcgttct ctggcgccgt gctcaccgtg agcggtgtgt
     1801 tcctcgccag cctctaccga tggccggcct tcgacgtccg cggcttcgag gtgctcaccc
     1861 acaagccgag catcgccgcc taccgagcgc ccgtcgcgcc gcacacgatc ttcgcgctcg
     1921 actcgcacat ggagcagctc gctcgcgcgg tgggtctcga tcccgtcgag ttccgcctgc
     1981 gccacctcca gcgcgaaggc gatcccatgg ccaacaacca gccctggctg agcaacggcg
     2041 ccgtcgaggt gctccagcgg accaccgagc acccgatctg gaagagccgg gcggaatgga
     2101 aggccagcgc cagggacggt cgcctgcgcg ggaccggcct cgcgctcggc ggctggctcg
     2161 gggggctgca gccgaccggc gccacggtgc ggctcaaccc cgacggctcg ctccaggtgc
     2221 tcacgggaca ggtggacatc gccgggacca acatcgcgct ggcccagatc gcggcaacgg
     2281 cctacggcgt ggacgtggac agggtccgta tcacgacggg agacaccgac gtcgcgcccg
     2341 tcaccggcct cagcgccggc agcaagaccg tctacaccgt cggcgtggcg gtgatggagg
     2401 ccgccaggga cgcccgtcgg cagaccatgg agatcgccgc caaggagctg gaggcgtcgg
     2461 tgcacgacct cgagatcgag gacgggcgcg tcgtcgtccg cggcgtgccc gatcgcggga
     2521 tcacgctggc tcagatcggc aagaagggca acctctacat gtcgaaggtg cccccggtcc
     2581 tcggcaagag ccacccggcg ttctcccagc aggcgccggc cttctgcgcc gagctggcgc
     2641 ggatcgaggt cgaccccgac accggcgagg tcacgatcca cgatttcgtc gtcgtccagg
     2701 acgtgggcaa ggcgatcaac ccgctcggcg tcgagggtca gatgcagggc ggcgccgtcc
     2761 agagcctggg gatggcgctg accgaagggc tgatgttcga cgcggccggc cggctgacga
     2821 acccgagcct gctcgactac cggaagctca cggcggccga cctgccggac atcgagacga
     2881 tcatcgtgga gaagccgtcg ccggcgggac ccttcggcgc ccgcggcgtc ggcgagccgc
     2941 cgatcgtgcc cgcgccggcg gccatcgcca acgccatcga ggacgcgacg ggcgtgcggc
     3001 tcaccgagct gcccctgacg cccgagcgga tcgcgctggg gatcgcggcg cgtcgagcgc
     3061 tcggatagcg ggctcgagct agacagggcc ggtcgcggag cgtcgccgac cttcgtcccg
     3121 gggccccgag cctgcggggc cggggcccca tgcggtcaac cgcgggaccg gaacactttg
     3181 cccccgaacg cgcggtcgaa gtggcgcgtg gcggcgccgt cgagactgcg ctggaacttc
     3241 cggtagcacg agaggaagtc gcggccggtg gacgtcagcc gcatgccgct tcgcgaggtg
     3301 ccctccggcg cgcgctccac gagcttgaac ccggcggcct tctcgagctc gcgcaggtag
     3361 ccccaggcgt tgcgatagga catccccagc tcggccgccg ccttccgcag cgacccgtgc
     3421 tcgccgatga gctcgagcag ccgggcgcgg ccgtcgccga acttcaggcg ctgtccgaag
     3481 acgacccaga ccctcagctt cggcttcacg gcccgacact gtagtacggc gcccacgggt
     3541 ttgcacccgc cggctgcccc tctggcgccg ccgctgcgac agacgctccg tccgttgtct
     3601 cggtcgcccc gccatctcga cgccgttacc ggtgcgtctc acggcatcgg gtcacgcccg
     3661 acgtccggct gtcccccgcg cgcgaccgtc atggcgtggc tgcccccgcg gcggtcgcgc
     3721 cgggccgggc ggggcttgat tatgtcgcgg cgagcctata taaagccgtc ttatatgctc
     3781 cggcgagccg tcatcgccgg tgcctcctgg aggaatgtcg cgatggctcg actgctgctg
     3841 gctgcggccg tcgttctcgc cctggttcat cccggtgaag ctcaggcgcc ctcgaccacc
     3901 gtcatcctgt ccaccaccac gagcacccag gactccgggc tgctcgacgt cctggtgccg
     3961 ctgttcgagc gcaagaccgg gcacagcgtg aagacgatct cggtgggcac cggccaggcg
     4021 ctggcgctgg cggcgcgggg cgaggccgac gtcacgctcg cccacgcccc gtcgctggag
     4081 aagaaatacg tggccgaggg caagatgtcg aaccgccggc tcgtcatgta caacgacttc
     4141 gtcatcatcg ggcccgagga cgaccccgcg aagatcaagg gcggcaagtg ctcgctggac
     4201 gcgctcgaga agatcgccgc ggcggggagc cggttcgtct ctcgcggcga caagtcgggc
     4261 acccacaacc tcgagctggc gctctggaag caggccgggg tgacgccggc ggcgccctgg
     4321 tacatcgagt ccgggcaagg catgggcgcg accctcggca tcgccgatga ccgcagggcc
     4381 tacaccatca ccgaccgcgc caccctgctg gcgttcagca agcgcctgcg cctgcagacg
     4441 atggtcgagg gcgaccgccc gctgctcaac atctactccg tcatggaggt caacccggcc
     4501 aacggtccga gggtgaacgc cgccggcggc aaggcgttcg ccgacttcat gctggcgccg
     4561 gagacccagg ccgtcatcaa gaccttcggg gtggacaagt acagccagcc gctgttcgta
     4621 cccatcgcgg gcaagaagga cgaggacttc taggacatgg gggccccgac atggccccca
     4681 gacccccagg gtggctcggc ccgccccggc gcagccgtgg cgcgcctcgt cacccggacg
     4741 ggctcctagc cggtggacct cgtcctcgcc ggcgtccggc aggccatcgc cctgctgttc
     4801 gcaggcgacg ccgaggtctg ggcgatcctg ctcctgtcgc tccaggtctc agggaccgca
     4861 acgctgctcg cgctcgggat cggggttccg gccggcacgg cgctcgcgct cacgcggttt
     4921 cccgggcgca cgctgctcgt cggcgccgtg aacacgggga tggggctgcc gcccgtggtc
     4981 gtcggcctgt tcgtgacgct actgctctgg cggagcgggc cattcggcga ctgggaggtc
     5041 ctctacacgc cggccgccat cgtgctggcc caggcggtga tcgcctcgcc gatcgtcacc
     5101 ggcatcacgc tggccgcagt gcagaacgtg cccgagccgt tccggctgca gctcctggga
     5161 ctcggcgcct cgcggctgca gatggtatcg gtggtcctgc gcgaggcgcg gctgccgatg
     5221 ctcgccgccg tcatggcggg gttcggcggc atcatctccg agatcggcgc ctcgatgatg
     5281 gtcggcggca acatcaaggg ccagacccgc acgctgacca cggcgatggt gctggagacc
     5341 ggaaagggca acttcgacat cgccatcgcc ctgtcgatcc tgctcctcac gctggtcttc
     5401 ctcgtcaact ggctgctgac cgtgctccag cagcgcgcgc attgacatgg ggggcccgag
     5461 atcgcgcgca tcagcgcgcg ccatgccggc tccgacgcgg agcgtataat cgcggcgatg
     5521 tcgcgtctcc tgctcgccct cgccgtgtgc tcgctggccg gcgcggtccc ggccgccgcg
     5581 caggaggtga cggtctctgt cgccatcagc atgaaggagg cgatcgagga catcggccgg
     5641 cgcttctccg ccgggcaacc gggactcgcg ctgcgttaca acttcggcgc ttccggcgaa
     5701 ctgcagaagc agatcgaggg gggcgcgccc gtcgacgtct tcgtctccgc cgcgcagcgg
     5761 caggtggacg agctggacag ggccgggctc ctgctcgccg gcacgcgagt ggtgttcgcg
     5821 aggaacgtgc tcgtcgcgct caagccgcgc gacggggtgc tcgacgtgcc cgggccctcg
     5881 gacctgctgg accggcgcgt gcagcgtatc gccatcggca gcccgaagac cgttccggcc
     5941 ggccagtacg ccgaggagag cctccgggcg ctcggtctgt gggaccggct caagcacaag
     6001 ctcgtccacg gcgagaacgt ccggcaggtg ctcgactatg tcgtccgcgg cgaggtcgac
     6061 atcggagtgg tctacgccac cgacggcgcc ctgcggctgg atcacgtgcg gctggccttc
     6121 gcattctccg aggacaccca ccggccgatc gtctacccgg cggccgtcgt cgcggccacg
     6181 cgtcagcccg tgctcgcgaa ggcgttcgtg gagttcctca ccgggcgcga ggcgcaggcc
     6241 gtgctgggca ggctcggctt cgagccggcg ccggccggcg cgcggtgagc ccggaggcgc
     6301 tctcggcgct cgccctctcg gtccgcgtgg cgacggtggc cacgctgctg aacgcggtga
     6361 tcggcatccc gctcgcatac gtcctcgccc ggcgcgcgtt ccgcggccgt acgctgctcg
     6421 acctgctggt cacgctgccg ctcgtgctgc cgccgacggt gaccggctac tatctgatcg
     6481 tgctgctcgg tcgccgcggc tggctcggcg gcccgctcta cgaactcacc ggctggaccg
     6541 tcgcgttcac ctggtacgcg gcggtggtcg ccgcgacggt gatggcgctg ccgctcttgg
     6601 tccgcacggc gcgtgcggcg atcgagtccg tggaccgcga gctggagcgg gccgcctgga
     6661 cgctcgggcg cccggaatgg cgcacggcgc tcgaggtcac gctcccgctc gcgcgcaacg
     6721 gcatcgtcgc cggcctcgtg ctcgccttcg cccgcgcgct cggcgagttc ggcgccaccc
     6781 tgatgctcgc cggcaacatt cccgggcgga ccaccaccgt gcccctcgcg atctacacgg
     6841 cggtgcagac gggggagcgc ggcgacgcgc tcgtgctggt ggccgccctc acggcgctgt
     6901 cgtgcgtggt cctcgtgctg gccggccggc tcggcgcacg ggcggcctga gggcgtcgtg
     6961 gcgcggctcg aggtggcgct cgtcaagcgg ttgccgggct tcgcgctcga cgcggcgtgg
     7021 agcgccggcg acggcgtggc tgtgctcttc gggccgtccg gcgcgggcaa gacgctgacg
     7081 ctgcagtgcc tggcggggct gctcaggccc gatgccggcc gcatcgtggt cgacggcacc
     7141 gtgtacttcg atgcggcagc cggcatcgac ctgccggcgc agcgccgtcg cgtggggtac
     7201 gtcttccagg gctacgccct cttcccgcac ctcaccgtgg ccgagaacgt cggcttcgga
     7261 ctccgcaatc gcccgcgcgc cgagcgggtc gagcgcacgc gcgcggtggt cgaacggctc
     7321 ggcctcggcg gcctcgaggg gcggcggccg gcggagctca gcggcgggca gcggcagcgc
     7381 gtggcgctcg gccgcgcgct cgccatcgat cccgtgctgc tgctgctcga cgagccgctc
     7441 tcggcgctcg acgcgccgct gcgccgggtg ctccgcgacg agctgcgggg ggtcctcgcc
     7501 gagtcgggcg tggccgccgt cgtcgtcacc cacgacttcg ccgaggccta ccggctcgcc
     7561 gaccgcgtcg tcgtctacga gggcgggcgc gtcatccagg ccgcgccccg ctcggagctg
     7621 ctctggcagc cggcctcgga ggcggtggcg cggatcatgg gaatccgcaa catcctgcgt
     7681 ggcaccgtcg tgaaggccac gcccgaccgc atccagctcc ggtggcgcgg ccagctcctc
     7741 gaggctgtca actcgccgac gcgcgcctac ctgccgccgc cggacagccc catcgcgttc
     7801 ttcatccgcc ccgaatacgt gcgcctcgtc cgcaaggacc gcggcgcgcc ggatgcgcag
     7861 caccacatga acctcatggc gggcgaggtc gtcggcgagg cagacttcgg cacgacgtgg
     7921 acgctgacga tccggctcga cgagccgggt gcgccggcgc agggcgagca cgacctggag
     7981 gtcgaggtgc cccgcctggt ctacgagatc ctcgagatcg agcgcgatcg gcgctggcag
     8041 ctctcgatcc accgcggctc gatccaggtg ctgccggcgt gatcgcagtc gggcccgcgg
     8101 ccagcgcctc gtgaccgacg ccgtgctcgc gctgcgagac gtggtcgtcg ggtactcggg
     8161 ggcgttctcg gtcagcgtcc cggtgctgga ggtggccgcc ggcgaggtgc tcgctctcat
     8221 cgggcccaac ggcagcggca agtccacgct gctgcgcgtg ctggccctgc tgcaggcgcc
     8281 ggacgagggc acggtcgcct tccggggccg cgccgtctct gtcgccgacg ggctcgacgt
     8341 gcgccggcgg atggcggtgg tcttccagca gccgctgctc gccgacatgc gcgtcgagga
     8401 caacgtggag ctggggctgc gcttccgcgg cgtcccgccg cgcgagcgac ggacgcgcgt
     8461 ggaccggtgg ctcgagcggc tccagatcgg cgccctcgcc ggccgtcccg cccgtacgct
     8521 gtcgggaggg gaggcgcagc gggtggcgct cgcgcgcgcg ctcgctcccg agcccgacgt
     8581 gctgctgctc gacgagccgt tcggggcact ggatgcgccc acccgggcca ccctcgtccc
     8641 cgacctggcc gccatcctga gatccgaccg ggtgacgacg gtcattgtca cccacgaccg
     8701 cggggaggct caggccctgg ccgaccgggt cggagtgatg ttcgggggcc gcctgcacca
     8761 ggtcgacgag acggcgcgcc tcttcggagc gccggcgtcg gaggaggtgg cgcgcttcgt
     8821 gggggtggag acgatccttg ccggggaggt cgtcgacgct gaggacggcg tcgccgtcgt
     8881 cgaagtgggc ggccgccgcg tggaggtggc ggcgatcgcg cagcgaggcg agcggctgcg
     8941 cgtggggatt cgcccggagg acgtgacgct gtcgacgggc gccgagcccg gcgcctcgag
     9001 cgctcggaac cacttcgtcg gcacggtcgt gcgcctgact gccggcagcc ccgtccgggt
     9061 ggtcctcgat tgcggcttcc ccctggtggc agccataaca gctcgctcgg ccgcggagtt
     9121 acggctcgcc gccggcgccc gggtgacggc cgtcttcaag gccagcgcgg cccacgtgat
     9181 ccgggccggc tcttcttgac accccaaccc ctccggcgat ataagtcaat cacccgcaac
     9241 caggagtatc ggacgggggt tggtattgac cgagctttcg ccggagccgg cgaccctggg
     9301 gacgccggct tcgtttgctt tccacccacc gctggttcac ccaccactgc ccaccgacgg
     9361 gaggccacac atgaatcagg agagcctcat cgagcgactg acggccgaga acgaggagtt
     9421 ccgccgcctg cgcaccgagc accaggacta cgaccgggag ctcgagacgc tgaagggtga
     9481 ctccacgctc tcggcccaac agcagtggcg catgaccgag ctgaagaagc tgaagctgat
     9541 ggccaaggac cgcatggaga acatcatccg gcacacccgc gtcaccgcct gacgccgggc
     9601 cgagccgacc agacttccat gccgcggaac gccgcgaccg cgctccgcgg tctcacgcat
     9661 ctcacaccgg cgggcgaggc ccgcatggtg gacgtgtccg ccaaggccga gaccgcccgc
     9721 gaggccgtcg cccgcgcgac cctcaggatg tcgccgcgga cgctccgcgt cgtccgggcc
     9781 ggcaacgcgc ccaagggtga cgtgctcggc gtcgcgcgca cggccggtat cctcgccgcc
     9841 aagcgcaccc acgagctgat cccgatgtgc catccgctgc gcgtgaccgg cgtcgacctc
     9901 gcgttcagcg acgacgtccg ccgcggcgag ctgacgatcg aggcgcgcgt gcgcaccgtg
     9961 gacaagaccg gcgtcgagat ggaggcgctg accgccgcga gcgtggcggc gctgaccgtc
    10021 tacgacatgg tgaaggccgt cgagaagggc gtgacgatcg agcgcgtgca gctcgtcgag
    10081 aagtcgggcg ggaagtcggg gacgtggaag aggcggacgt gaagacggcg ctgatctact
    10141 ccgacgagtg gacccgcttc gactacgggc ccgagcaccc gctccggatg gaacggctcg
    10201 ggttgacgtg gaagctgatg gaggcctacg gcctcacgtc gctgccgacg ggcgccgtca
    10261 cggcgcccga tcccgccacc gaggcggagc tgctgaagtt ccactccgag gagtacctga
    10321 cggtgctgaa ggcggcgaac agcggggtcg cgccggccgc tgtgatctgc cactacgggc
    10381 tcggccccgg cgacaatccg gtcttcccag gtctctggga ggcggcgcgg ctggtcgccg
    10441 gcgggtcgct gctcggagcg cgcctcgtcg ctggcggcga ggtccagcgc gcctttcact
    10501 tcgcgggcgg gctgcaccac gccatgaccg atcgcgcctc gggtttctgc tacgtcaacg
    10561 acgcggtcct ggccatcctg catctgagga cgcagggatt gcgggtggca tacgtcgaca
    10621 tcgacgccca tcacggtgac ggtgtgcagg ccgcgttcta cggcgagcgc gacgtgctca
    10681 ccatctccac ccacgagcgc ggcgaccggc tcttccccgg caccggattc gtggaggaga
    10741 tcggcgaggg ctccggccgc ggcttctccg tgaacctgcc gctcgacgtg ctcaccgacg
    10801 cgtccgtcta tctgccggca ttcgagcgcg tggtgccgtc cctgctgaag gccttccgtc
    10861 ccgacgtgat cgtcgcgcag ctcgggatcg actcgcaccg caccgacccg ctcacgcacc
    10921 tggcgctcga cgtccagggc ttcgcgcgcg cggtcgcgcg gatcgccgag ttcgccccgc
    10981 ggctgctggc gctgggcggc ggcggctacg acctcgggaa cgtcgcccgc gcctggaccg
    11041 ccgcgtgggc ggtcatgaac ggcgtcgagc tgcccgccga gatcccggcc ggcttccgcg
    11101 actacgcccg ctggctgcgc gtccgtccgg cctcgctgtg ggacccgccg gcggtgatcg
    11161 atgacgagcg ccggcgcctc gcccgggagt acgtggaccg tcaggtcgac aagatcgagc
    11221 ggctgatctt cccgctgcac ggcctctgag cgccgccccg cccctgagcc gctcgggtcg
    11281 cctcgtctcg cctctcgcca gctggctccg tgataccgcc accgtgcgcc gcttccgtcg
    11341 cggcctcggc cgcgcgccgg tcgtgctacc gccgcgcgat accgcgtggc ggtccatcgc
    11401 gccgggattc gaggaggcgc gcgagatggc ggcctcgggc ctgcccgtgc agatcgctgc
    11461 ggcgcgccgc tacgaccgcg ctcccgaccg tcggcgcatc gccagcgcgc tggtccgcgg
    11521 cgagaccgtg ttcatgccgc aggtgcacca ggtgctgccc cgggtggcgc gtctcatggt
    11581 cgccatccga gtgatcttga tgggcccgtt cagagaggag tgctcgttcc tgttcctggt
    11641 cggcggcggc ggtcgcgagg gcatggggct ccaccacgat gggccagtgg actcgttctg
    11701 gcttcagctc gccggccggc gcacggtgac gatcgggccc cgcgtggagc ccgggtcgcc
    11761 ggaagacatc gaggcgcccg ggtggggccg tggctggcag accttcgacc tcccaccggg
    11821 cacgctgttc tacctgtcgc cctggacgcc gcaccgggtg gtgtgccgcg tgcgctcgct
    11881 ggcgctgtcg ctcacgtggt cgccgccgcg gcgcgccgca accgcgggcg gcgtgcgctc
    11941 gcgcccggcg accgcgcggg cgcggaccgc ggcggtccgg gcgcgcgcgg aatcgctgtc
    12001 tcggtgggac gtggtcgacg gccgcgtgac ggcgcgcccc gggcgcagtt ccggtcggct
    12061 ctggacccag gtcccggcca tcgcgggtgc gcccgatgcg cgtggccgct tcaggctcct
    12121 gacgcccgac ggcgcgctcg tcctcgatcg cgcggcgcgc ccgcttgccg gtaggctcgc
    12181 cgacatgccg gccctggagc gggcggccgt cgatggccgt gctgctctgg ccccgcttct
    12241 ggcccacggc attctcggcg acgaggacct gccacggcgc atcgtcccag cagacccgga
    12301 ggggctcgac ggctggcgct ttgcgtagcg gctcccgaac aacacatcgt ctcctcaccg
    12361 cggtcgtcgt cgcctgtagt gcgcagatcg accgcctcgc cgaacgatac gcttgccgac
    12421 ggtccccgat gccaccgggt tcggcgagcc cgacggcctg atgccgtacg cccccgctga
    12481 catcgaccct gcctgccgtg cggcgtgcta cagtgacttg atctcagcgg ctggaggctc
    12541 gggcgtgcgg ggagtgctca tcacgtccga gggcgtcgag ggttcgggaa agacgaccca
    12601 gatggcgcgc ctcgggcggt ggctcgagaa gcaagggtac cgggtcgagt gcacgaggga
    12661 gcctgacggc acgccgctgg gcgcggcgat ccgccggctc ttcgagcgtc ccggcgtccg
    12721 gccgctcacc gaggtcttcc tcttcatggc cgcgcgccag cagcacgtgg ccgagaggat
    12781 cgcgccgtgg ctcaagcgag gcgccgtcgt gctgtgcgac cgctacaccg atgcgacggt
    12841 cgcctaccag ggctacgggc gtgggatcga ccgcgacctc atccgcgagc tgaacgtccg
    12901 tgccaccggc ggcatcctgc ccgacctcac cctgctcttc gacctcgatc cacgcgaggg
    12961 gttccggcgg atcggccggc gccggctcga ccggttcgag cgcgaggcgc tggctttcca
    13021 ccggcgggtg cgccgcggct atctcgagat ccagcgggcg gagcccaagc gcgtgcggct
    13081 cgtccgcgcc gggcgcccgc cggcagtcgt cgccaccgag gtcgaggcga tggtaggaga
    13141 atttttccgt ggcacttgag gcgatccgcg accagccgcg cgtggtggac ctgctgcggc
    13201 gggcgctcgc ctccgggcgc gtcgcgcacg cgtacgcctt cctcggcgcg ccgggcagcg
    13261 ggcggacgac ggccgcgcgg gcgttcggcg ccgcgctcct gtgcgagcac ggtggctgcg
    13321 gggcctgccg gggctgcacg ctggcggctg cgggccggca ccccgacctg cacgtgatcg
    13381 tgccgacgcc gccggagaag aaccccaggg gcccgctcgc cgtcaggatc gacgacatcc
    13441 gcgcgctcga gcacgcggcc gcgctggcgc cggtgatggc cccgcggaag gtcttcgtgc
    13501 tggaggacgc cgaccgcatg acgggcgaga cgccgcaggc cttcctcaag acgctggaag
    13561 aaccgccgcc gcggacggtg atgattctcg tgttgcagcg cgcgcgggcc gtgccggcga
    13621 cggtgctctc gcgctgccag atcgtgcgct tcgcgccgcc gccggccgcg gcggcgccgg
    13681 agcgggcgga ggcgttggag gcgctgctcg aggtgcgggg caagggcgcc gacgcgctct
    13741 tccgtcgctc gcaggtgttc gaccgcgacc gcgagcgcgc cgagcgcttc gtggacggct
    13801 gctggctcct gtgccgcgac ctcctgctgg cgaaggccgg ggcgccgccg cgcctgctcg
    13861 tgcggcccga gcaggccgag gtgctggcgc gcgaggccgg ggactggagc caggacgcgc
    13921 tcctggcgag catcgcggcg tgtcgcgagg cgcgcgcggc gctcgcggtc aacgtgacgc
    13981 cccggctgac gctggagatc gtgctgggcc gcctcgcctc gcgggcagcg tgagagggcg
    14041 atgagcgatc aggccctgaa cgacacggcg gtggccgagt atctcgtcgg cgtccgcctc
    14101 cgcgaggtgg cccagacgga ggaccatcgg ctcgccggcg acgacctgat ccctcacgtc
    14161 ggcgacctcg tgatcgtcga tacgggctcc ggcgaggcgg tcgccgaggt gcggcggccc
    14221 cggcgggcga tcccggaggc caagcgcggg cgcgggtggc cgcgcatcgt ccgcgccgcc
    14281 aacgagaccg aggctcgcga atggcgcgag cggctcgagc gcgagaagaa ggcggtcgtg
    14341 accagtcagc ggctggcgcg ggcggccggg ctcgtcatga agatcgtgga cgtcgagatg
    14401 caccccgccg cccgccgcgt gaccgtctac ttcaacgcgg acgagcgcgt ggacttccgc
    14461 cagctcgtcc gcgacctcgc ccgcgagttc cgctcgcgca tcgagatgcg gcagatcggc
    14521 gcccgcgaca cgaccaaggc catggacggc atcggcccgt gcggccggca gctctgctgc
    14581 tcgtcgtacc tccgcaagtt cgagccgatc tcggtgaaga tggccaaggc ccaggacatg
    14641 ccgctcaccg acagccggct gctcggcaac tgcggccggc tcaagtgctg cctgctctac
    14701 gagttctcgc tctaccagga gctgcgcgcg cggctgccga aggtcaacac gccctgccag
    14761 gcctcgtgcg gcggcggagg gtgcatgagc ggcaagatca agtcgctgcg cgtgctcaag
    14821 cagtcggtgg tggtgggctt tcccgatggc accgaggccg aggtcccgct cgaccaggtg
    14881 acgtgggagg gacggcccca cgtccaggcc cagctcgaca agacgtgagc gcccgccggt
    14941 gagcacgttc tacctgacga cgccgatcta ctacgtcaac gcgccgccgc acctcggcca
    15001 cgcgtacacc atggtcgtcg gcgacgcgct cacgcgctgg caccgcatgg ccggcgacga
    15061 cgtgctcttc ctcaccggca ccgacgagca cggcgacaac atcgcccagg ccgcggccag
    15121 ggcgggcgtc acgccgcagg cgctggccga ccagaactcc gcggccttcc gtcgcgcctg
    15181 ggacgagctg gggatccggt acgacgacta catccgcacc acggagccgc gccacaaggt
    15241 ggtcgttcag gcgatcctcc agcggctctg ggacgcgggc gagatctacc tcggccgcta
    15301 cggcggacac tactgcttcg gctgcgagcg gttctacacc gagaaggaga tcgtcgacgg
    15361 gcagtgtccc gaccaccaga ggccgctcac ctgggtcgag gaggagaact acttcttcaa
    15421 gatgtcgaag taccaggact ggctggtcgg ctacatccac gatcacccgg acctgatccg
    15481 gcccgagcga taccgcaacg agatcctcgg cttcctgcgc gatccgctgc aggacctctc
    15541 catcagccgg ccgaagaagc ggctgcagtg ggggatcccg ctccccttcg acgacgacta
    15601 cgtcacctac gtctggttcg acgcgctgat caactacgtg tccgcgcttg gcgggccggg
    15661 ggccccgaag ttcgactcct actggccgca cgcgcggcac ctgatcgcca aggacatcct
    15721 caagccccac gccgtctact ggccctgcat gctgaacgct gccggcctcg aggtgtatcg
    15781 ccagctcaac gtgcacggct actggacggt gggcgggcgc aagatgtcga agtccgtcgg
    15841 caacctggtg gacgcgctgg ccgtgaagga ccggttcggc aacgacgcct tccgctactt
    15901 cgtgatgcgc gagatgacgt tcggcctgga cgccgacttc tccgaggagc ccttcatcgg
    15961 ccggctcaac gccgacctcg ccaacgacct cggcaacctc gtctcgcgcg cgacgacgct
    16021 gatcgtcaac ggcggcggca tctcgcgggt gccggaggcg cgtgagctcg gcgagctgga
    16081 agccgacgtg ctgggcggct tcgacaaggc gcgccaggag ctggacgcgg ccatggccga
    16141 cttcgcgttc cagcgggcgc tcgcggcgat ctggggatgg atcggccggc tcaacggcta
    16201 cgtggacgcc acccagccgt gggccctcgc gaagcaaccg gcgcagcggg cgcgcctgga
    16261 ggtcgtgctc tacacgctcg ccgagtcgct tcggctgctc ggcatcgtgc tcgcgccctt
    16321 cctgcccgac gccgcgtcga agatccgcgc ggccctcggc gagacgggcg agctgcggct
    16381 ggcggacgcg acatggagcc gcctggccgc cggtgcccgg atccagaagc tctccggcct
    16441 cttcccccgg gtggacgccg cgcgcggagt ggaagcccct ggagccgccg gggaggttgg
    16501 ggtcgggcca gccacggagg cggccgggcg agcggggccc gtcacgacga ccccggtggt
    16561 ggcgcccgag cgctcgatcg tccgcatcac cgacttccag aagatcgacc tgcgcgtggc
    16621 cgaagtcgtc ggcgcggagc ccgtgccgaa gtccaagaag ctggtcaagc tcacggtctc
    16681 gctcggcgac gagcggcgca cggtcgtggc cggcatcgcc gagcactacg cgccggcgga
    16741 cctcgtcggc aagaaggtcg tggtcgtcgc gaacctcgag ccggcgacgc tgatgggcgt
    16801 ggagtcgcag gggatgctgc tcgcgggctc cgccgaaggg gcgctcgcgg tgctcacgct
    16861 cgaccgggac ctgccgccgg gggcgaaggt gaggtgaccc gatgaccgac gtcgacgtca
    16921 tgatccaggt cgccgggcag gagccatcca tcgcgcgggt gctgcgcgag atctgcgcgc
    16981 tggagggcgg cgtgcgatcg ctcgccctcg acctcgtcgc cgcgcagctc aggacgcacg
    17041 ccaggtcccg agacctcctg gaggcgatcg cggcgctgcg cagagacgac gtcgcgcgcc
    17101 gcatcgccga ggcgctggcc aggacgcgtt gagcgtcgag acagagccgc aggcgctggc
    17161 gcaaccagcc gtcgaggcag gtgcgctcgt ccggaggggc cggaacaacc cgctggtgcc
    17221 cttcttccgc cgatggggcg gctcgatcgc gtccagcgtc ggcggcctgc tcacgctcct
    17281 cgtctttcgc cacggggtgg ctcacgtccg gtggatcgtg ggctatctgc tcctcgcgtg
    17341 gatcatctcg gcgatggtcg tccagatcca ccgcgcgatc gtcgccgccg agcgccgggt
    17401 gcaccggctc gtcatcgccg ccgcggacta tgcgatccag agcctccacc acggcgtgct
    17461 gctgttcctg ttgccgccgt actgggcgag cgtcacgctg acctccgtca acgcgccgtt
    17521 cttcgtgctc ctcgtcggat tcgtggtggt ggcgacggtc gatccctggt atcgcgcgct
    17581 cgtccagccc cggccgtggc tgaccgctct cttcttcttc gtctcgacgc tcggagccct
    17641 gaacctggcg ctgccgctcg tcggcgtccc gccgcacacc gcgctggcgc tcgccgccta
    17701 cgtcgcgacg gtcgcgctga cgccgctcct cgtccatgcg cgcggctggc cgtggctgca
    17761 cgcggtggga ggcaccgccg tcgccggcgt cgccctggcc gcgctcgtct acgccggccg
    17821 ggccggcgtg cccccggctc cgttgttcgt ggccgagaag cgattggcgt ggaacttcgc
    17881 gatggccgac tacctccagc cggtggccag ggcgatcccc acccgcgagc tgcgcgagcg
    17941 cggtctcgtc gtccacacgg cggtgtacgc gccggccggg ctgacgcagc cgatccggca
    18001 cgtgtggcgc cagcacggca aggtggtgga cgacgtgacg ctctcgcccg tgcgcggcgg
    18061 ccgcttcgag gggttccgca ctttttcccg caagacggcc tggcccgcgg acccgcgagg
    18121 caagtggacc gtcgacgtcg tgaccggcgc cggccagctc ctcggccggc tgtcgttccg
    18181 ggtaacggga tgacgcccga cctcttcgac acccacgcgc acctgcactt ccccgacttc
    18241 gcgccggacc tgccggcggt cctcgagcgc gctcgggcag cgggcgtgcg gcgctgcctc
    18301 acgatcggca cggacgtcga gagctcgcgc gcggccgtgg ccctcgccgc gcgggaaccc
    18361 gacgtgtggg cctcggtcgg tatccacccc cacgacgcgg cggccgcgga cgacgcggcg
    18421 ctggcggaga tcgcgcgtct ggcgcgcgcg ccgcgcgtcg tcgccgtggg cgagatcgga
    18481 ctcgactggt tccgcgacct ctcgccgcgc ccggcgcagg aggcggcctt ccggcgccag
    18541 ctcgcgctgg cgcgcgagat cggccggccg gtactcgtgc actgccgcga ggcacacgcc
    18601 gacgtgctgc gcatcctcgg cgaggagcat gccgtcgacc gcggcggcat catgcactgc
    18661 ttctccggcg acgtcgcgat cgcgcggcga tgcctcgagc tgggctttct cgtctcgctg
    18721 gccggcccgg tcacctatcc caaggcgcgc gccctgccgg aggtcgcgcg ctggactccc
    18781 ggcgaccgac tcgtgatcga gacggactgt ccttacctcc cgccgcaggc gcaccgcggc
    18841 cagcgcaacg agccggcgta cctcgcgctc acggccgcgc gcgtggccga gctgcgcggc
    18901 gagccgctgc cggagctcgc cgcgcggctc accgccaacg cctgtgcgat gctgggggtg
    18961 ccgtgagtct ccgcgacgtc ggcttctacg tgcgagggcg cgtcagctcc ggcgtggcgc
    19021 gggcgctcaa ccgtcgccgc gcggtgacgc tccctccgcc ggtgccgctg acggaggtgc
    19081 tccgcgggcc gggctcgtcg tcggcgctgg tccggctcct cgagggccgt gccaatcacg
    19141 agcgggaggc ggcggtggcg gcgtggctcg cggcacgcgg cgtgccgttc gcgcgtcacc
    19201 ggttcgccac gttcgagggc cgcggcgaga atctcggcgt cgagatcggc tcgggcgacc
    19261 gcgtgctcgt gctcatcgcc caccacgacg cggtgcccgg cagccccggc gccaacgaca
    19321 acgccgcggc cgtggcgatc ctgctcggtc tcctgccgcg gctcgagcgg gtcgtccctc
    19381 ctggtctgcg cgtgcggctg ctcttcatgg cgtgcgagga gctgggctac ctcggcgcgc
    19441 gcgcctacgt gcgcgacacg ccgctcggct cgatcgtcgg cgtgctgagc ctggagctct
    19501 gcggcatcgg cgacagcctc gccctgtggg acgcggtgga ggagacgccg ttccggcggc
    19561 gggtcaccg
//
LOCUS       DATIAZ010000224         6286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_456116, whole genome shotgun sequence.
ACCESSION   DATIAZ010000224 DATIAZ010000000
VERSION     DATIAZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456116"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VLF19_07920"
     CDS             <1..396
                     /locus_tag="VLF19_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93218.1"
                     /translation="AARRALGRHWEGRTEGQRAEFVRLFTELIDRGYLSKLDLYEGER
                     LAWAGESVQGDEAVVKTQVVSKSGEMPADFLMTRGADGRWQVRDVVVAGVSLVGNYRT
                     QFAKILRATSFDELLAKLRTKHAAAGGRD"
     gene            complement(491..1393)
                     /locus_tag="VLF19_07925"
     CDS             complement(491..1393)
                     /locus_tag="VLF19_07925"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HSE93219.1"
                     /translation="MRSEGPAIEPRLLRGRARYERVMAGSTDDAPGGALAHTVVLRDD
                     DRALELTAMALPSPTYELREARCRVLAGEVAPEAVRGVTALAGTGMVAGFTRRVVEAT
                     GGGEGAGLVVDAAVEVARLARQVAKLPRGCAERAVGSDPWECWQLDTTGWADLPDSCF
                     TYSAAGRALFGTRPVTSPMTPDLYSPRPGQRRVFERRKRARLERRDGRLVLFHAMHDT
                     VHGFELTCEIDLASGRVLRADHITPRLPYMGICTEPQTRLQSLVGEVADAGLRKRIGP
                     LLGGPTGCAQLYDLTADLLKLLAA"
     gene            complement(1383..1544)
                     /locus_tag="VLF19_07930"
     CDS             complement(1383..1544)
                     /locus_tag="VLF19_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSE93220.1"
                     /translation="MAIDPVCKMSVEPAKAAAQSTHQGQTYYFCAVGCKQKFDREPEK
                     YLGSGGGAI"
     gene            complement(1556..3808)
                     /locus_tag="VLF19_07935"
     CDS             complement(1556..3808)
                     /locus_tag="VLF19_07935"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSE93221.1"
                     /translation="MERRTYPVRGMHCAACVGKVEQALRSVPGVAEASVNLATERASV
                     EVDPGRADFPALRRAVEAAGYMLAEAAATPGADSEQERREHEQRRARARFLVGAVLSV
                     PVFLGGMTALLPWVPPWLASPWTLLVLTTPVQFWVGWEFHAGLLRDLRHRTASMATLV
                     SIGTSAAYFFSVAVTLWPNTFGAHGHMTHFETSAVVITLVVLGRWLEARARGRTSEAI
                     RRLMSLAPRTARVVRDGHDVEVPTGEVTVGDLVRIRPGERLPVDGTVVEGASAVDESM
                     LTGESLPVEKTLESKVVAGTVNRTGSFVFRATRVGSETTLARIVRLVEEAQGSRAPIQ
                     RLADRVAAVFVPIVLGIAALTFVGWALFGPEPRLVMALTTSVAVLVIACPCAMGLATP
                     TAIIVATGRGAEHGVLIKSAAALELLHRVDTVVFDKTGTLTVGRPEVTDVITLGDWAE
                     PEVLALAAAAEQGSEHPLGEAIVRRAKDLGLALPPIEAFDTVPGLGVDALTPDGRILL
                     GNGALLEARGVEIPSSAAERAAALQVVGKTVVYLAFAGRLEALVAAADVLKPEAAATV
                     ARLRALGVDVLMLTGDNRRTADAIARKAGITRVLAEVLPEAKAGEIARLRDAGRLVAM
                     VGDGINDAPALASAGVGIAMGSGTDVAIEAADVTLMRGDLFGVTAAIELSRRTMRIIR
                     ENLGWAFGYNVVLIPVAAGVLYPLWGMLLSPILAGAAMAFSSVSVVTNSLRLKTGSPR
                     HASSTVGREG"
     gene            complement(3817..4098)
                     /locus_tag="VLF19_07940"
     CDS             complement(3817..4098)
                     /locus_tag="VLF19_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HSE93222.1"
                     /translation="MVDETTKTKALGRLRRIEGQVQGLQRMIAAEAYCVEVLLQISAV
                     QGALEQVQKLLLGRHIESCVTDALRSGTKGDRQRKIDELLDVFSRFGGK"
     gene            complement(4187..4600)
                     /locus_tag="VLF19_07945"
     CDS             complement(4187..4600)
                     /locus_tag="VLF19_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93223.1"
                     /translation="MARPRSPRVSATKILRELRTAAKKGDPAALTLALAEMRTLALSP
                     RYWEKYLALLGNPLARLVDLLVIKQGERIAHQKGWKRSKGGFRPPSSSKGASTALSEA
                     STRGWRGQSPRSKRRPNARRRQAVAAAEQQSLFEM"
     gene            complement(4602..5657)
                     /locus_tag="VLF19_07950"
     CDS             complement(4602..5657)
                     /locus_tag="VLF19_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HSE93224.1"
                     /translation="MARDLPLLPTTVCGSHALPSWIFLVREATEAGRLGPTDLQEAYD
                     DAVRLAIRDQVEAGVDVISDGEMRRVTFIRGFYGRLTGIEPLPVPRRLGPVNYDTFCP
                     YQVVGKIEAPQGLGVVDEFRFARPHVDRTLRVAVPGPMTLLLALRRGGPYGTEDALVA
                     DLAAIVNREARGLAEAGCELVQIDEPNYVMMAGKHRVLRAGPGPMLEALGAAVRGVSA
                     KLALHVCFGNAHNNSFATPRHYAPLFPQIVGAPVHQLVFEYANREMAEIELWSAHRTD
                     KEVAVGVVDVKAFRVETAEEIAARARLALKHLPAERLWLVPDCGLWETPRWVAVSKLR
                     SMVEAARLLRRERSGRA"
     gene            complement(5684..>6286)
                     /locus_tag="VLF19_07955"
     CDS             complement(5684..>6286)
                     /locus_tag="VLF19_07955"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HSE93225.1"
                     /translation="YIVIDHTEALVSIDVNTGKYVGKRDFEQTVLKINLEAVGEIVRQ
                     IRLRDLGGIIIIDFIDMEVLEHRDQVYRALKKALADDKARTNVLEISELGLVEMTRKR
                     VRQDLRSLLTMSCPTCRGSGVTKSDATLAAEIFRAVQAKAGESGAREIVVRAHPEVVA
                     YLEGDAREGLERLRAQLDVKVIVQTAGGHPHREEYQIQPR"
BASE COUNT          905 a         2278 c         2228 g          875 t
ORIGIN      
        1 gcagcccggc gcgcgctcgg ccgccactgg gagggccgga cggagggcca gcgcgcggag
       61 ttcgtgcggc tcttcaccga gctcatcgat cggggatacc tctcgaagct cgacctttac
      121 gagggcgagc gcctggcgtg ggcaggggag tcggtgcagg gcgacgaggc ggtcgtgaag
      181 acgcaggtgg tcagcaagtc cggcgagatg ccggcggact tcctcatgac acgaggagcc
      241 gacggccgct ggcaggtccg cgacgtcgtg gtcgcgggcg tcagcctcgt gggcaattac
      301 cgcacccagt tcgccaagat cctgcgcgcg acgtcgtttg acgagctgct ggcgaagctt
      361 cgcacgaagc acgcggcggc aggcggtcgc gactgagtcc aggggggctc cgggcgtccc
      421 cgaactttcc ggggcgtgcc gcccagccgt ggcgtccctc gacaacgcga ttcgttcaca
      481 gactctgggg tcaggcggcc agcagcttca gaaggtcggc ggtcaggtcg tagagctggg
      541 cgcagccggt cggtccgccg agcaagggtc cgatccgctt gcgcaggccc gcgtcggcga
      601 cctcacccac cagcgactgc aggcgcgtct ggggctccgt gcagatgccc atgtacggca
      661 gccgcggcgt gatgtggtcc gcgcgcagga cgcgcccgct ggcgaggtcg atctcgcacg
      721 tgagctcgaa gccgtgcacg gtgtcgtgca tggcgtggaa gagcacgagc cggccatcgc
      781 gccgctcgag tcgcgcgcgc ttgcgccgct cgaagacgcg ccgctgcccc ggccgggggc
      841 tgtagaggtc gggcgtcatg ggcgacgtca ccggccgcgt gccgaacagc gcacggcccg
      901 ccgcgctgta ggtgaagcag gagtcgggca ggtcggccca gccggtcgtg tcgagctgcc
      961 agcactccca cgggtcggag ccgaccgcgc gctcggcgca ccctcgcgga agcttcgcca
     1021 cctggcgggc caggcgtgcc acctcgacgg cggcgtcgac gacgagcccg gcgccctcgc
     1081 cgcctccggt cgcctcgacg acccggcgcg tgaagccggc gaccatgccg gtgccggcga
     1141 gcgcggtgac gccgcgcacg gcctccggag cgacctcgcc ggcgagcacg cggcagcgcg
     1201 cctcgcgaag ctcgtaggtg ggcgacggca gcgccatcgc cgtcagctcc agcgcgcgat
     1261 cgtcgtcgcg cagcacgacc gtgtgcgcca gcgcgccccc gggcgcgtcg tcggtggagc
     1321 ctgccatgac gcgctcgtag cgggcgcggc cgcgcaggag gcgaggctcg atggccgggc
     1381 cctcagatcg caccgccacc gctcccgaga tacttctccg gctcgcggtc gaacttctgc
     1441 ttgcacccga ccgcgcagaa ataataggtc tggccctggt gggtggactg cgccgccgcc
     1501 ttggcaggct cgacgctcat cttgcagacg ggatcgatgg ccataatgca cctcctcatc
     1561 cttcgcgacc gacggtcgac gaggcatgcc gtggagagcc agtcttcagg cgcaggctgt
     1621 tggtgacgac cgacacggac gagaaggcca tcgcggcgcc ggcgagaata ggtgagagca
     1681 gcatgcccca caacgggtac aggacgccgg ccgcgacggg aatcaacacg acgttgtagc
     1741 cgaacgccca cccgaggttc tcgcggatga tccgcatcgt tcgccgcgac agctcgatcg
     1801 ccgcggtgac gccgaagaga tcgccgcgca tcaacgtcac gtcggcggcc tcgatggcca
     1861 cgtcggtacc cgagcccatg gcgatgccga cgccggcgct ggccagcgcc ggcgcgtcgt
     1921 tgatgccgtc gccgaccatg gccacgaggc gaccggcgtc gcgcaggcgc gcgatctcgc
     1981 cggccttcgc ctccggcaac acctcggcca gcacgcgggt gatcccggcc ttccgcgcga
     2041 tggcatcggc cgtacggcgg ttgtcgccgg tgagcatgag gacgtccacg ccgagcgctc
     2101 gcagccgcgc cacggtcgcc gccgcctcgg gcttgagcac gtcggccgcg gcgacgagcg
     2161 cctcgagccg tccggcgaag gcgaggtaca ccacggtctt acctacgacc tgcagcgcgg
     2221 cggcgcgctc ggccgcggag gacgggatct ccacgccgcg cgcctccagc agcgcgccgt
     2281 tgccgaggag gatccgaccg tcgggggtga gcgcgtcgac gccgagcccc ggcacggtgt
     2341 cgaaggcctc gatgggcggg agcgcgaggc ccagatcctt cgcgcgccgc acgatggcct
     2401 cgcccagcgg atgctccgat ccttgctcgg cggcggcggc gagcgccagg acctcgggct
     2461 cggcccagtc gccgagcgtg atgacgtccg tcacctcggg ccggccgacc gtgagcgtgc
     2521 ccgtcttgtc gaacacaacg gtgtcgaccc tgtgcagcag ttccagcgcc gcggcgctct
     2581 tgatcagcac gccgtgctcg gccccccggc cggtagccac gataatcgcg gtcggcgtgg
     2641 cgagccccat cgcgcaggga caggcgatga cgagcacggc caccgacgtc gtcagcgcca
     2701 tcaccagccg aggctcggga ccgaacagcg cccagcccac gaacgtcagc gcggcgatgc
     2761 cgagcacgat cggcacgaac accgcagcca cgcggtcggc gagccgctgg atcggggcgc
     2821 gggagccctg ggcctcctcg acgagacgga cgatgcgggc cagcgtcgtc tcgctgccga
     2881 cgcgggtggc gcggaagacg aagctgccgg tgcggttgac ggtgccggcc accaccttcg
     2941 actcgagcgt tttctccacg ggcaggctct cacccgtcag catcgactcg tccacggccg
     3001 aggcgccctc gaccaccgtg ccgtccacgg gcagtcgctc gccgggccgg atgcgcacga
     3061 ggtcgccgac cgtgacctca ccggtcggca cctcgacgtc gtggccgtcg cggacgacgc
     3121 gggccgtgcg cggtgcgagg ctcatgagcc gccggatcgc ctcggacgtg cggccccgcg
     3181 cgcgagcctc gagccagcgg ccgaggacca cgagggtgat gacgacggcg ctggtctcga
     3241 agtgggtcat gtgcccgtgc gcaccgaagg tattcggcca cagcgtcacc gcgacgctga
     3301 agaagtacgc cgcgctggtg ccgatcgata cgagcgtcgc catgctcgcc gtgcggtggc
     3361 ggaggtcgcg gagcaagccg gcgtggaact cccatccgac ccagaactgc accggcgttg
     3421 tcaggaccag gagcgtccac gggctggcca gccagggagg cacccagggc aggagagcgg
     3481 tcatcccgcc gaggaagacc ggaaccgaca gcacggcgcc gacgaggaat cgcgcgcgcg
     3541 cccggcgttg ctcatgctcg cgccgctcct gctcgctgtc ggcgcccggc gtcgcggcgg
     3601 cctcggccag catgtagccc gccgcctcga cggcccgccg gagcgccggg aaatcggcgc
     3661 gcccgggatc gacctcgacg ctcgcgcgct cggtggcgag gttcaccgaa gcctcggcga
     3721 cgccggggac cgaccggagc gcctgctcga ccttgcccac gcaggcggcg cagtgcatgc
     3781 cgcgcacggg gtacgtccgg cgctccacgg ccggcctcac tttcccccga agcgggagaa
     3841 gacgtccagc agctcgtcga tctttcgctg ccggtcgccc ttggtgcccg accggagcgc
     3901 gtcggtcacg caggactcga tgtggcgccc gaggaggagc ttctgcacct gctcgagggc
     3961 cccctgcacc gccgagatct gtagcaggac ctcgacgcaa taggcctcgg cggcgatcat
     4021 ccgctgcagc ccctgcacct ggccctcgat gcgccggagc cgccccaggg ccttcgtctt
     4081 cgtcgtctcg tcgaccacct ataccctcgg ggggtattat cccagccccc gcccccctgt
     4141 caagctgccg ccggccccct gcgtccgcgg cgccgaatcc ggagcctcac atctcgaaca
     4201 ggctctgctg ctccgccgcc gcgaccgctt gccgacggcg cgcgttgggc cgccgcttcg
     4261 agcgcgggct ttgcccgcgc catcccctgg tggaggcctc ggagagggcc gtcgaggccc
     4321 ccttcgacga ggatggggga cgaaaccccc ctttggatct cttccatcct ttctgatgcg
     4381 cgatccgctc gccctgcttg atgacgagca ggtcgaccag gcgtgcgagc gggttgccga
     4441 gcagcgccag gtacttctcc cagtaccgcg gcgacagcgc gagcgtgcgc atctcggcca
     4501 gggccagcgt cagcgctgcc gggtcgccct tcttcgccgc ggtccggagc tcgcgcagga
     4561 tcttagtggc cgacacgcga ggcgaccgcg gccgtgccac gtcaggccct gccggaacgc
     4621 tcgcggcgga gcagccgcgc ggcctcgacc atggaccgga gcttggagac ggccacccag
     4681 cgtggcgtct cccacagccc gcagtccggt acgagccaga gtcgctcggc cggcaggtgc
     4741 ttgagggcca ggcgcgcgcg cgcggcgatc tcctccgccg tctccacacg gaacgccttg
     4801 acgtccacca cgccgacggc gacctccttg tccgtccggt gcgcgctcca cagctcgatc
     4861 tccgccatct cgcggttggc gtactcgaag acgagctggt gcaccggcgc gcccacgatc
     4921 tgcgggaaga gcggcgcgta gtggcgcggc gtggcgaagg agttgttgtg ggcgttgccg
     4981 aagcagacgt gcagggccag cttcgccgac acgccgcgca cggccgcgcc gagcgcctcg
     5041 agcatcgggc ccgggccggc ccggagcacc cggtgcttgc cggccatcat cacgtagttc
     5101 ggctcgtcga tctgcaccag ttcgcacccc gcctcggcca ggccacgtgc ctcgcggttg
     5161 acgatggccg cgaggtcggc gacgagcgcg tcctcggtcc cgtagggccc gccgcgtcgc
     5221 agcgccagca ggagcgtcat ggggccgggc acggcgacgc gcagcgtgcg gtcgacatgc
     5281 gggcgggcaa agcggaactc gtcgacgacg ccgagtcctt gcggcgcctc gatcttgccc
     5341 accacctggt acgggcagaa cgtgtcgtag ttcaccggtc ccaggcggcg cggaacgggc
     5401 agcggctcga tgccggtgag ccgaccgtag aagccgcgga tgaaggtgac ccggcgcatc
     5461 tcgccgtcgg agatcacgtc cacgccggcc tcgacctggt cgcggatggc cagtcggacg
     5521 gcgtcgtcgt acgcttcctg gaggtccgtg gggccgagcc gtccggcctc cgtcgcctcg
     5581 cggacgagga agatccacga cggcagcgcg tgcgatccgc acaccgtcgt gggaagtagc
     5641 ggcaggtccc gcgccatgac ggcgcgatgc taccacgccc cgatcagcgc ggctggatct
     5701 ggtactcctc gcggtgagga tggccaccgg ccgtctgcac gatgaccttc acgtcgagct
     5761 gcgcccgcag gcgctccagc ccttcgcgcg cgtctccctc gagataggcg acgacctcgg
     5821 ggtgggcgcg cacgacgatt tcccgcgccc ccgactcgcc ggccttggcc tggacggcgc
     5881 ggaagatctc cgcggccagg gtcgcgtcgg acttggtgac cccgctgccc cggcacgtcg
     5941 ggcaggacat cgtgaggagc gacctcaggt cctggcgcac ccgcttccgt gtcatctcga
     6001 ccagccccag ttccgagatc tcgagcacgt tcgtccgcgc cttgtcgtcg gcgagcgcct
     6061 tcttgagcgc ccggtagacc tggtcgcggt gctccagtac ctccatgtcg atgaagtcga
     6121 tgatgatgat gccgccgagg tcacgcaggc ggatctgccg gacgatctcg ccgacggcct
     6181 ccagattgat cttgagcacg gtctgctcga agtcgcgctt gcccacgtac ttgccggtat
     6241 tgacgtcgat cgagacgagc gcctcggtgt ggtcgatcac gatgta
//
LOCUS       DATIAZ010000225         8790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1507262, whole genome shotgun sequence.
ACCESSION   DATIAZ010000225 DATIAZ010000000
VERSION     DATIAZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507262"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..976)
                     /locus_tag="VLF19_07960"
     CDS             complement(5..976)
                     /locus_tag="VLF19_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93226.1"
                     /translation="MNATGRRAFLSSMALGAAVAGAPRTARAQPSVKVGTAVLGDYAL
                     AGPFIVARERGFFTQEGLEVEFVPFRGGPDLVKAVIAGEILLGAAGSTDILVFREAGM
                     PLKMVATQTEGNHFTLNVLPEIVRAADLKGKVIGVTRAGATTWVFARMIARENGWDPD
                     RDVKIVGLGGLDAQLAALARKEIHAFVWGDGGAVTEQQGKSKVLMRLDRVTPRWISQI
                     QYASEDGIRKHDEHLRKAMKAVFRGQRLMREKPEDAAADAAKGLGWTPEATLGAHKIS
                     GALMSADGTISVEALRTMQDTLLDHGVIKKRLPLEEHYTKEFVPVKV"
     gene            1056..1844
                     /locus_tag="VLF19_07965"
     CDS             1056..1844
                     /locus_tag="VLF19_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSE93227.1"
                     /translation="MSDAPVGYGVRDGVAWITLSRPSVLNALNTELAAALADRVEAAT
                     ADADVMLVVVRGAGRAFCSGMDRTALARGAIGEPFYRHWIRALNGLEDMPKITLAVLH
                     GYSIGGGLQLALACDLRLATEDAVLGLGATRHGLVPDGAVLRLARVVGLGRAKELALL
                     NDDVSPAEARAMGLVNWVCAPAALDAAVESILARARSASPTAAGHTKRLLHASFHADP
                     RAIIEEVVRAQNDCMASWEMAEANRAWQEKRQAVFHPRGKPEGS"
     gene            1841..2626
                     /gene="menB"
                     /locus_tag="VLF19_07970"
     CDS             1841..2626
                     /gene="menB"
                     /locus_tag="VLF19_07970"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HSE93228.1"
                     /translation="MKYTDILYEKKDGVAWITINRPEVRNAFRTRTVHELTDAFMDAR
                     FDPAVGVVVLTGAGDKAFCSGGDQKERGTGGYSADGDRPMYVDGLHTAIRHCPKPVIA
                     MVNGFAIGGGHVLHVLCDLSIASDNAIFGQTGPRVGSVDAGHGTGYLARVVGEKKARE
                     IWYLCRQYTAQEALAMGLVNKVVPLKDLRSEVEQWCRELLEKSPTALALAKQSFNIDS
                     EPRAGVAEFASTALNLYYQTEEAMEGRNAFVEKRSVNFRRFRK"
     gene            complement(2741..3874)
                     /locus_tag="VLF19_07975"
     CDS             complement(2741..3874)
                     /locus_tag="VLF19_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HSE93229.1"
                     /translation="MRTVKSHGRFQVVAILALFGLIVLYSLAPAPAGGQAKPIVLKVQ
                     ASFPASSLIYTSVLPVFAERMEKASGGRVKIEALAAGTIVPAFEVVDATNRGVIDGAF
                     TCTYYYIGKHKAAALFTDVPGGPFGLDTYDYMSWHYQGGGVQLLNEFYQQVLRMDIVA
                     WPIFPTTPQAFGWFKKPIKSAKDLRGLKYRVPGLAADVYKAMGAAVVTLPGGEILPAG
                     ERGVLDATEWYTPGEDIRLGFHQVWKFYHYPGPHEMAGACDLLLKKSVFDKLPADIQE
                     IVKSVTLETAFRSLMEFNRKDAEGLRDLKEKHGVTLVRTPQDVMTEHFKAWDRIAAEE
                     AGKDPFFKKVLDSQRAYAALVVPARRFMSSYEMTANYYYGEMK"
     gene            4477..5838
                     /locus_tag="VLF19_07980"
     CDS             4477..5838
                     /locus_tag="VLF19_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HSE93230.1"
                     /translation="MPTRAPLATEIRAGATTFMVMAYIIFVNPLVLGFAGVPGLEGKG
                     LPLTATLTVTCLTAGVLSIAMGLITNYPFALAPGMGLNAVVAFELVAGRGLTWPQAMT
                     VVFLEGVVITLLVLTRFREAVMDAVPVGLKQAISVGIGLFIAFIGFFTAGFVVKPAAG
                     PLPVGLGSLGGLPIAVFVVGFVLTAWLAARRVQGALLVGILATTALAIVLNGLVGDWK
                     AFPTPGAAQIPTAIVRVPDFSTFGRLDFGLFAAIGALAAALAIFSIMLSDFFDTMGTI
                     IGVGGKGGFLDKQGRLPRANRVLLVDSLGAAVGGYANASSNTTYIESAAGVAEGARTG
                     TAAVTVGVLFLLAMFFSPLAAVIPAQATAPALILAGFYMMSIAREIPWDDYEEAVPAF
                     VTMLTMPFTWSITNGIGAGFVTWTAIKVLGGRGGRVHWMVYLATLAFVVYFVLPLIEK
                     ALR"
     gene            5857..6129
                     /locus_tag="VLF19_07985"
     CDS             5857..6129
                     /locus_tag="VLF19_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93231.1"
                     /translation="MLAANLAGTSLANTGAESTEASFRGGAVRIGLLRFQGETVAFVQ
                     AGDLQALALRPVWEVPTTMYLSPDELPALANAIARLAARVKQLRGS"
     gene            6164..6508
                     /locus_tag="VLF19_07990"
     CDS             6164..6508
                     /locus_tag="VLF19_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93232.1"
                     /translation="MILVHAVLAAGLLLAARTAAAESRVFELTIRGGELPEPSRVVRV
                     RQGDDVTLRWTTDKPVTIHLHGYDLEQKLAPGKPVAMRLTAGATGRFPIEIHAHGSGG
                     HRTIAYLEVHPR"
     gene            6522..8081
                     /locus_tag="VLF19_07995"
     CDS             6522..8081
                     /locus_tag="VLF19_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93233.1"
                     /translation="MVRPGALAWRATGTLLLTAVAARPAGAHGFGQRYDLPVPLWLWV
                     AGAAAAVLLSFVVIGLFVRGNPGARGYPRANLLRSTIGRLLADRRVRRAAQAVSVGLL
                     VLVVATGLFGDQNPTRNLAPTFVWVIWWVGFAYVSALAGNLWAVVNPWAATFGGAEAL
                     VRRLGGDELALGWPYPRGLGMWPAIVLFAAFGWAELVYGGRAIPAQLALMVTVYSLIT
                     WAGMALFGRGVWLRHGDPFAAAFGLLARFSPTEIRVTDAGACAGCADACRPGRDGCHD
                     CGGCFERASPAGREWNLRPYGAGLLRTDDVSPSMVVFVLLLLSTVAFDGFTATPAWAA
                     LESALYAALAPLGGARLTVIGTLGLLGFAVAFAAVYALFAVAMARAGEGALSTGAVAQ
                     VFVLSLVPIAIAYHLAHYFTYLLIQGQLVIRLASDPFGFGWNLFGTARYRPDIGIVGA
                     RFAWYTAVIAIVLGHIIAVWIAHVIALRRYPDRRTAIRSQVPMLVLMVGYTIVSLWII
                     AQPIVESSPKG"
     gene            8211..8435
                     /locus_tag="VLF19_08000"
     CDS             8211..8435
                     /locus_tag="VLF19_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93234.1"
                     /translation="MESPLGRQALRPGTAYWLPGLTPHDTRNEGQRPARMLEILLKRC
                     DGPSEAAPRGISRGVHRAYGVRSIASTMRE"
     gene            complement(8396..8659)
                     /locus_tag="VLF19_08005"
     CDS             complement(8396..8659)
                     /locus_tag="VLF19_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HSE93235.1"
                     /translation="MAVYMVERELKGITMDQLAAAQKAAIDTGKKLTAQGKQVRYIRS
                     TFVPGEARCMCLFEADDQQHVRELNDSAKIPYSRIVEAMDLTP"
BASE COUNT         1186 a         3086 c         3041 g         1477 t
ORIGIN      
        1 gccatcacac cttcacgggg acgaactctt tcgtgtagtg ctcctcgagc ggcagccgct
       61 tcttgatgac gccgtggtcg agcagcgtgt cctgcatcgt ccggagcgcc tcgacgctga
      121 tggtgccgtc ggccgacatc agcgcgccgg agatcttgtg ggcgcccagc gtggcctccg
      181 gcgtccagcc gagccccttg gccgcgtcgg ccgccgcgtc ctcgggcttc tcgcgcatga
      241 gcctctgccc gcggaacacg gccttcatcg ccttcctcag atgctcgtcg tgcttgcgga
      301 tcccgtcctc ggacgcgtac tggatctggc tgatccagcg cggcgtcacg cggtccaggc
      361 gcatcagcac cttcgacttg ccctgctgct cggtgacggc gccgccgtcg ccccagacga
      421 aggcgtggat ctccttgcgc gcgagcgcgg cgagctgggc gtcgaggccg ccgaggccca
      481 cgatcttgac gtcacggtcg ggatcccagc cgttctcgcg cgcgatcatg cgcgcgaaga
      541 cccacgtcgt cgcgccggca cgcgtgaccc cgatcacctt gcccttgagg tcggcggcgc
      601 ggacgatctc cggcaggacg ttgagggtga aatggttgcc ctcggtctgg gtggcgacca
      661 tcttcagcgg catgccggcc tcgcggaaga cgaggatgtc cgtgctgccc gcggcgccca
      721 gcaggatctc gccggcgatg accgccttga caaggtccgg gccgccgcgg aagggcacga
      781 actccacctc gagcccctcc tgggtgaaga acccgcgctc ccgggcgacg atgaacggcc
      841 cggccagcgc gtagtcgccg aggacggccg tgccgacctt cactgagggc tgggcgcgcg
      901 cggtacgggg agcgccggcg acggccgcgc cgagcgccat ggaggagaga aaggcacggc
      961 gaccggtggc attcatgtga gtcctccggg gcgggcatcg cttgacactg tgtcgagttt
     1021 cccataggtt aggcggcact ttccatgatt caatcatgag tgacgcgccc gtcggttacg
     1081 gtgtccgcga cggcgtggcg tggatcacgc tgagccgccc ttccgtcctc aacgcgctga
     1141 acaccgagct cgccgccgcg ctggcggacc gcgtggaggc ggcgaccgcc gacgcggacg
     1201 tcatgctcgt cgtggtgcgc ggcgccgggc gtgcgttctg ctcggggatg gatcgcacgg
     1261 cgctggcgcg cggcgccatc ggcgagccct tctaccgcca ctggatccgc gcgctcaacg
     1321 gtctcgagga catgccgaag atcacgctgg ccgtgctcca cggctactcc atcggcggcg
     1381 gcctgcagct cgccctcgcc tgcgacctca ggctggccac ggaggacgcg gtgctcgggc
     1441 tcggcgccac ccgccacggc ctcgttcccg acggcgctgt gctgcggctg gcgcgagtgg
     1501 tggggctcgg ccgcgcgaag gagctggcgc tgctcaacga cgacgtgagc ccggccgagg
     1561 cgcgggcgat gggcctcgtg aactgggtgt gcgcgccggc cgccctcgac gccgcggtgg
     1621 agtccatcct cgccagggcc cggagcgcgt cgccgaccgc cgccggacat accaagcggc
     1681 tgctgcacgc gtcgttccac gcggacccgc gcgcgatcat cgaggaagtg gtccgcgccc
     1741 agaacgactg catggcctcg tgggagatgg ccgaggccaa ccgcgcctgg caggagaagc
     1801 gccaggcggt gttccacccg cgcggaaaac cggagggctc atgaagtaca cggacatcct
     1861 ctacgagaag aaggacggcg tggcctggat caccatcaac cggcccgagg tgcggaacgc
     1921 tttccgcacg cggaccgtgc acgagctgac cgacgccttc atggacgcgc gcttcgaccc
     1981 ggccgtcggc gtggtcgtgc tgacgggcgc gggcgacaag gcgttctgct ccggcggcga
     2041 ccagaaggag cggggcaccg gcggctactc cgccgacggc gaccggccga tgtacgtgga
     2101 cggcctgcac accgccatcc gccactgtcc caagccggtc atcgccatgg tcaatggctt
     2161 cgccatcggc ggcggccacg tgctgcacgt gctgtgcgat ctctcgatcg cctccgacaa
     2221 cgccatcttc ggccagaccg gaccccgcgt gggcagcgtg gacgccggcc acggcacggg
     2281 ctacctcgcc cgcgtcgtgg gcgagaagaa ggcgcgggag atctggtacc tctgccggca
     2341 gtacaccgcc caggaggcgc tcgccatggg cctcgtcaac aaggtcgtgc cgctcaagga
     2401 tctgcgatcc gaagtcgagc agtggtgccg ggagctgctc gagaagtcgc ccacggcgct
     2461 cgcactcgcc aagcagtcgt tcaacatcga ttccgagccc cgggcgggcg tcgcggagtt
     2521 cgcctcgacg gccctcaacc tctactacca gacggaggag gccatggagg gacggaatgc
     2581 gttcgtcgag aagcggtcgg tgaacttcag gagattccgc aagtagggcc ggtggcgccc
     2641 tccgccagcg ccggcttctc gtggatccga gccgccgtcg agcgctcgaa gccggtagac
     2701 tgccggtggc tccgagcgct tggcagctga cgggaggcgt ctacttcatc tcgccgtagt
     2761 aatagttcgc ggtcatctcg taggagctca tgaagcgtcg agccggcacg accagcgcgg
     2821 catacgcccg ctgggagtcc aggaccttct tgaagaaggg atccttgccg gcttcctccg
     2881 cggcgatcct gtcccacgcc ttgaagtgct cggtcatgac gtcctgcggc gtcctcacca
     2941 gggtgacgcc gtgcttttcc ttgaggtccc gcagcccttc ggcgtccttc cggttgaact
     3001 ccatgagcga gcggaacgcg gtctccaggg tgacggactt gacgatctcc tggatgtcgg
     3061 cgggtagctt gtcgaacacg ctcttcttca gcaggagatc gcaggccccg gccatctcgt
     3121 ggggcccggg atagtgatag aacttccaga cctggtgaaa gccgaggcgg atgtcctcgc
     3181 ccggggtgta ccactcggtc gcgtcgagca caccgcgctc gccggcaggc aggatctcac
     3241 cacccggcaa cgtgaccacg gccgccccca tggccttgta gacgtcggca gcgagccccg
     3301 ggacgcggta cttcagtccc ctgagatcct tggcgctctt gatcggcttc ttgaaccacc
     3361 cgaaggcctg aggcgtggtg gggaagatcg gccaggcgac gatgtccatc ctgaggacct
     3421 gctggtagaa ctcgttcaag agctgcacgc cccctccctg gtaatgccag gacatgtagt
     3481 cgtacgtgtc caacccgaac ggcccgccgg gcacgtcggt gaagagcgcc gccgccttgt
     3541 gcttgccgat gtagtagtag gtgcacgtga aagccccgtc gatcaccccg cggttggtgg
     3601 cgtccaccac ctcgaaggcg gggacgatgg tgccggccgc cagggcctcg atcttgacgc
     3661 ggcctccgga ggccttctcc atccgctcgg cgaaaacggg gaggaccgag gtgtatatca
     3721 ggctcgatgc cgggaagctg gcctggacct tcaggacgat gggcttggcc tggccccctg
     3781 cgggtgcagg cgcaagactg tagaggacga tgagtccgaa cagtgcgagg atggcgacta
     3841 cctggaatcg accgtggctc ttcactgtcc tcactccctt ctcggcgcgc tcagctcggc
     3901 gccgtgtgcc cggggcgctg ccagcccggc tacgccctct gtcggtaacc cgccgtcgca
     3961 cccaccagtc tggcaggatg cgcggaattt ccctcgtcgc ctcgtgtgtc cggggcctga
     4021 cgtcgtgcgt ggctcctccc acgcggctga gggagactgg ccgtggccga gatcggtggc
     4081 atcgcggacg gagccagagc gagcggcgac gagcattgcc ggacgtcgag aggttgtcaa
     4141 ggcacgcttc gtcaccacca cccgtgtgtc cgcgtgttcc cgccgttcta tcccggctgc
     4201 gtcgcgcgct actcggaaat attgcgcccc ggcggcccct gcgcattggt gcccatcccc
     4261 gtcgacaaca tcgaccggcc gccgagaggc cacggccgga cgtcaacggc cttgcggttt
     4321 gctcgatcga gttgccgtcg acacggcgcc gtcggtggcc gagctgcgcg gctgccacaa
     4381 ggggccgacg gtcggccgag acccgaccgc cacccaggcc gcgcgggaca acgtgctggc
     4441 caagctcgcc gagctgttac ggtaggaggc gccaccgtgc cgacgcgcgc cccgctggcg
     4501 accgagatcc gagccggcgc cacgaccttc atggtcatgg cctacatcat cttcgtgaac
     4561 ccgctggtcc tcggcttcgc cggcgtgccg gggctcgagg gcaaggggct gccgctcacc
     4621 gccacgctga ccgtcacgtg cctcaccgcg ggcgtgctct cgatcgcgat gggcctgatc
     4681 accaactacc ccttcgccct cgcgccgggg atggggctca acgcggtggt cgccttcgag
     4741 ctggtggccg gccgcgggct cacatggccg caggccatga cggtcgtctt cctcgagggc
     4801 gtcgtgatca cgctgctggt gctcacgcgc ttccgtgagg cggtgatgga cgccgtgccc
     4861 gtcggtctca agcaggcgat ctcggtgggg atcggcctct tcatcgcgtt catcggcttc
     4921 ttcaccgccg gcttcgtggt gaagccggcc gccgggccgc tcccggtcgg cctggggtcg
     4981 ctgggcggtc tgccgatcgc cgtcttcgtt gtgggcttcg tcctcaccgc ctggctcgcg
     5041 gcgcggcgcg tgcagggcgc gctgctcgtc ggtatcctcg ccaccaccgc gctggcgatc
     5101 gtcctcaacg gcctcgtcgg cgactggaag gcgttcccca cgccgggcgc ggcgcagatc
     5161 cccaccgcca tcgtgcgagt gccggacttc tccacgttcg gccggctcga cttcggcctc
     5221 ttcgcggcga tcggcgcgct ggcggccgcg ctcgcgatct tctcgatcat gctgagcgac
     5281 ttcttcgaca cgatgggcac gatcatcggc gtcggcggca agggcgggtt cctcgacaag
     5341 cagggccggc tgccgcgcgc caaccgcgtc ctgctcgtgg actccctcgg cgccgccgtc
     5401 ggcggctacg ccaacgcgag cagcaacacg acctacatcg agagcgccgc cggcgtggcc
     5461 gagggcgcca ggaccggcac cgccgcggtc accgtcggcg tgctgttcct gctggcgatg
     5521 ttcttctcgc cgctcgccgc ggtcatcccc gcgcaggcca cggccccggc gctcatcctc
     5581 gccggcttct acatgatgtc gatcgcgcgc gagatcccct gggacgacta cgaggaggcg
     5641 gtgccagcgt tcgtcacgat gctgaccatg ccgttcacgt ggtcgatcac caacggcatc
     5701 ggcgcgggct tcgtgacgtg gacggccatc aaggtcctcg gcggccgggg cgggcgcgtg
     5761 cactggatgg tctacctcgc gacgctggcc ttcgtggtct acttcgtgct gccgttgatc
     5821 gagaaggcgc tccggtgatg cggtactctg gggatcgtgc tcgccgccaa cctcgccggg
     5881 acgtcgttgg cgaacaccgg cgccgagagc acggaagcga gctttcgagg gggcgcggtg
     5941 cgcatcggcc tgctgcggtt ccagggcgag accgtggcct tcgtgcaggc gggcgatctt
     6001 caagcgctgg cgctccggcc ggtctgggag gtaccgacca cgatgtacct ttctccggac
     6061 gagttgccgg cgctggccaa cgccatcgcc cgactggcgg ccagggtcaa gcagctgcgt
     6121 ggctcctgac ccggttcgga gaccacgcct gcagcgacgg cgcgtgattc tggtgcacgc
     6181 cgtgctcgcg gccggcctcc tgctcgcggc acggacggcg gccgcggagt cgcgggtctt
     6241 cgagctgacc atccgcggcg gcgagttgcc cgagccctcg cgcgtcgtgc gcgtccgcca
     6301 gggtgacgat gtcacgctgc gctggacgac ggacaagccg gtgacgatcc atctgcacgg
     6361 ctacgatctc gagcagaagc tcgcgccagg caagccggtc gcgatgcggc tcacggccgg
     6421 ggcgacgggc cgctttccca tcgagatcca cgcccacggc tccggcgggc accggacgat
     6481 cgcctatctc gaagtccatc cgcgctgacg cggtcggcgg catggtccgg cccggagccc
     6541 tcgcgtggcg ggcgaccggc acgctgctgc tgacggccgt cgccgcccgt cccgcgggcg
     6601 cccacgggtt cgggcagcgc tacgacctgc cggtgcccct gtggctctgg gtcgccggcg
     6661 cggccgctgc cgtccttctc tccttcgtgg tcatcggtct cttcgtccga ggcaatccgg
     6721 gcgcccgggg gtacccgcga gcgaacctcc tccgctcgac gatcggtcgt ctcctcgctg
     6781 atcgccgcgt gcggcgcgcg gcgcaggccg tgtcggtcgg cctcctcgtc ctcgtcgtgg
     6841 cgacggggct gttcggagac cagaatccga cgcgcaacct cgcgcccacc tttgtctggg
     6901 tgatctggtg ggtcggcttc gcgtacgtgt ccgcgctggc cggcaacctc tgggccgtgg
     6961 tgaacccgtg ggcggctacg ttcggcgggg ccgaagcgct cgtccgccgt ctcggcggcg
     7021 acgagctggc gctcgggtgg ccgtacccgc gagggctcgg gatgtggccc gccatcgtcc
     7081 tcttcgctgc gttcgggtgg gccgagctgg tctatggcgg ccgcgccatc cccgcacagc
     7141 tcgcgctcat ggtcacggtc tactcgctga tcacctgggc cgggatggcg ctcttcggcc
     7201 gcggcgtgtg gctgcgccac ggcgatccgt tcgccgcggc cttcggactg ctcgcccggt
     7261 tctcgccgac agagatccgc gtgacggatg cgggcgcctg cgccggatgt gcagacgcgt
     7321 gccggcccgg ccgtgacggc tgccatgact gcggcgggtg cttcgagcgc gcctcgcccg
     7381 cgggccggga gtggaacctg cgcccctacg gcgccgggct cttgcgtacc gacgacgtgt
     7441 cgccgtcgat ggtcgtgttc gtgctgctgc tgctgtccac cgtcgccttc gacggcttca
     7501 ccgcgacgcc ggcctgggcg gcgctggaga gcgccctcta cgcggcgctg gcgccgctcg
     7561 gcggcgcgcg cctgaccgtg atcggcaccc tcggtttgct ggggttcgcg gtggcgttcg
     7621 ctgctgtcta cgccctcttc gcggtcgcga tggcccgcgc cggcgagggc gcgctgtcca
     7681 ccggcgccgt cgcccaggtc ttcgtgctct cgctggtccc gatcgcgatc gcctaccacc
     7741 tggcgcacta cttcacctat ctgctgatcc agggccagct cgtgatccgg ctcgcctccg
     7801 atcccttcgg cttcggctgg aatctcttcg gcacggcgcg ctaccgccct gacatcggta
     7861 tcgtgggcgc ccgcttcgcg tggtacacgg cggtgatcgc catcgtgctc ggacacatca
     7921 tcgccgtgtg gatcgcccac gtgatcgcgc tccggcggta tccggaccgc cggacggcca
     7981 tccggagcca ggtcccgatg ctcgtgctca tggtcggcta cacgatcgtg agcctctgga
     8041 tcatcgccca acccatcgtc gagtcgagcc cgaaggggta gtggggcgca ggtcatcctc
     8101 gacaatcctt cggtgcgcgt cagcgtcatc acgcttcccc caggcggcgg aaccgggcgg
     8161 catcagggga tcgaggcgga agtggcatcg tcgtcgaggg agacctgatc gtggagagcc
     8221 ccctggggcg ccaggcgctg cggcccggga cggcctactg gctgcctggc ttgacgcccc
     8281 acgacacccg caacgaaggc cagcggccgg cgcgaatgct ggagatcctg ctgaagcggt
     8341 gcgatggacc gtccgaggcc gcgccgcgcg ggatctcacg cggcgtgcat cgcgcctacg
     8401 gcgtcaggtc catcgcctcc acgatgcgcg agtaggggat cttcgcggag tcgttcagct
     8461 cacgcacgtg ttgctggtca tcggcctcga agagacacat gcagcgcgcc tcgcccggta
     8521 cgaacgtgct gcggatgtag cggacctgct tgccctgcgc ggtgagcttc ttgcccgtgt
     8581 cgatcgcggc cttctgggcc gcggcgagct ggtccatcgt gatgcccttg agctcgcgct
     8641 ccaccatgta gacggccatg gctgacacct cccgtgatgc ggctcctgtt agcatgcggc
     8701 ggcgcagtgg tcaagcaacg gtcaacaaca gcagcggtcg ccgaaggcca cggccattgt
     8761 gggctgcaac gatcgtgagc ctctggatca
//
LOCUS       DATIAZ010000226         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_851336, whole genome shotgun sequence.
ACCESSION   DATIAZ010000226 DATIAZ010000000
VERSION     DATIAZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851336"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VLF19_08010"
     CDS             complement(<1..215)
                     /locus_tag="VLF19_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93236.1"
                     /translation="MAHRLRLDVEMKHEEKPPAVGDLPRSGNGHGASTFDVRGVASAP
                     EAATARGDRLRRWLPLGVLALAALLLVA"
     gene            complement(310..522)
                     /locus_tag="VLF19_08015"
     CDS             complement(310..522)
                     /locus_tag="VLF19_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93237.1"
                     /translation="MAARKKTRKTVMPSPRSAKAGRPPREVPASSQVRRRRKTATAAV
                     RKDSMVQQTAEARIWWGVGKPKERKR"
     gene            complement(613..1025)
                     /locus_tag="VLF19_08020"
                     /pseudo
     CDS             complement(613..1025)
                     /locus_tag="VLF19_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013982306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
     gene            complement(1075..2274)
                     /locus_tag="VLF19_08025"
     CDS             complement(1075..2274)
                     /locus_tag="VLF19_08025"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HSE93238.1"
                     /translation="MIVLVLNCGSTSVKFAVIATGHASARRLAAGRVERVGGEATVVF
                     EADGGATVRSSEVLADHVDAVRRVVDWVTEAARLPRIEAVGHRVVHGGDRFTTPAVVD
                     DAVVSAIEALETLAPLHNAPSLAGIRAARATLGADVPMVAAFDTAFHAALPEAAARYA
                     IPDDLARRHRVRRYGFHGLAYRSVLAGYARAAGVSPDAARIVALHLGGGCSAAAIAGG
                     RSLDTSMGFTPLEGLVMTTRSGDLDPALVDHLAAREGVPAAEVVGWLNQRSGLLGLSG
                     TSGDVRDLLAREAHDPRARLALEVFVHRARKYVGAYLAVLGGADAVVFSGGIGEYSAT
                     VRTRIGAGFGWCGLALDEARNAAVAGGARRISADGARLAAWVVPADEETVIAEDTVTR
                     VAARSIA"
     gene            complement(2345..2692)
                     /locus_tag="VLF19_08030"
     CDS             complement(2345..2692)
                     /locus_tag="VLF19_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSE93239.1"
                     /translation="MLLPGIRVSSVWAALGAGLVLGLINAVVRPILLVLTLPVTLLTL
                     GLFLFVLNGLCLWLTAAIVDGFEIAGFWWAVLGAVLVSLVSWVLTAFVSDAGRIVLLG
                     GDGPPARGRRAGA"
BASE COUNT          388 a         1056 c         1017 g          338 t
ORIGIN      
        1 gcaacgagca ggagcgcggc gagcgcgagg acgccgaggg gaagccagcg gcgaagccgg
       61 tcgcctcggg cggtcgccgc ctccggcgcc gaggcgacac cacgcacgtc gaacgtcgac
      121 gcgccatggc cgttgccgga acgcggcagg tcgcccacag ccggcggctt ctcctcgtgc
      181 ttcatctcga cgtccagccg caacctgtgt gccacccggg acagccgcga aatcgctcgg
      241 ttggctaacg ccccgcgcag gcttgcgggt acgacccccc cggggtgggg cagcggcgcc
      301 ccagagccgt cagcgcttcc tttcctttgg cttgccgacg ccccaccaga tgcgagcctc
      361 tgcggtctgc tgcaccatgc tgtccttacg gaccgccgcc gtcgccgtct tgcgccggcg
      421 ccggacctgt gatgacgcgg ggacctcgcg gggcgggcgc ccggccttgg ccgagcgcgg
      481 ggacggcatc acggtcttgc gcgtcttctt cctggctgcc attgacggct cctctcggcg
      541 atgaccgcgg agtacggctc gttcgggctc gcggcgccag ggcactggtg gcgccgatca
      601 tgatcgccaa cggccggcgt cctcggctcg ccgccagtcg cggcgcccag tcgctgcccc
      661 gctgctcctc gaaggcgccg tcggacaggg acgcgacgag ccgctcgccc ggcagcggca
      721 tgtgcacgta ctgcagctcc gcgaacccga gatagcgccc tcggcgagac cgccggccgt
      781 gtacgcgttc acgtggctgc cgagcggcga ctccggctcg ccgtccgtgc gcagccggta
      841 cgcgtagaaa tcctggacgt accgggtcaa cccctcctcg gtgacgtcgt agcgcgccgc
      901 gtgctcgggc agagtgttgc cgagcaggag gttcaccgag tcgatggcgg ccacgcggtg
      961 gcctggccca tcagccagcc gcgcgtgagg ccggtgagcg cgttggccgc cgtgtagccg
     1021 gtgtatcccc ccgggctcgg gtgatatcct cgtgcggcag cgcccgcgtg gcgctcaggc
     1081 gatgctccgc gccgccacgc gcgtgacggt gtcctcggcg atgaccgtct cctcgtcggc
     1141 gggcacgacc caggcggcga gccgggcacc gtcggcgctg atccgccgcg ccccaccggc
     1201 gaccgcggcg ttgcgcgcct cgtcgagcgc gaggccgcac cagccgaagc ccgcgccgat
     1261 gcgggtgcgc acggtggcgc tgtactcgcc gatgccgccg ctgaagacga ccgcgtcggc
     1321 acctccgagc accgcgagat aggcgccgac gtacttgcgc gcccggtgga cgaagacctc
     1381 cagtgccagc cgcgcgcgcg gatcgtgcgc ctcccgcgcg agcaggtccc gcacgtcgcc
     1441 gctcgtgccc gacagcccga ggagccccga gcgctggttg agccagccga cgacctccgc
     1501 cgccggtacg ccctcgcgcg cggcgaggtg gtcgaccagg gccgggtcga ggtcgcccga
     1561 ccgcgtcgtc atcaccaggc cttcgagcgg ggtgaagccc atcgacgtgt ccagcgagcg
     1621 gccgccggcg atggcagcgg cggagcagcc gccgccgagg tgcagcgcga cgatgcgggc
     1681 ggcgtccggc gacactccgg ccgcgcgcgc atagcccgcc agcaccgagc ggtaggccag
     1741 gccgtggaag ccgtagcgcc ggacacggtg gcggcgcgcg aggtcgtcgg ggatcgcgta
     1801 gcgcgccgcc gcctccggca gcgcggcgtg gaaggcggtg tcgaaggccg ccaccatcgg
     1861 cacgtcggcc ccgagcgtcg cgcgggcggc acggatgccg gcgaggctcg gggcgttgtg
     1921 cagcggcgcc agcgtctcca gcgcctcgat cgccgagacg accgcgtcgt ccacgacggc
     1981 cggcgtcgtg aagcggtcgc ccccatgcac gacacgatgg cccacggcct cgatgcgcgg
     2041 cagccgcgcc gcctccgtca cccagtccac gacccggcgg accgcgtcga cgtggtcggc
     2101 aaggacctcg ctcgagcgga cggtggcgcc gccgtccgcc tcgaagacca cggtcgcctc
     2161 gccgcccacg cgctcgacgc gcccggcggc gaggcggcgt gcgctggcat ggcccgtggc
     2221 gatgacggcg aacttcaccg acgtgctccc gcagttgagc acgaggacga tcacggtagc
     2281 ctcgaccgcc aagggacgag tgcgcgggcg ccgcagtcgg gcggctggac ggcggagcgc
     2341 gtcttcacgc gccggcccgc cgtccccgcg ccggcggccc gtcgccgccg agcagcacga
     2401 tcctccccgc gtcgctgacg aacgcggtca ggacccagct cacgaggctg accagcaccg
     2461 cgccgagcac cgcccaccag aagccggcga tctcgaagcc gtccacgatg gccgccgtga
     2521 gccagaggca gagtccgttg aggacgaaca ggaacagtcc gagcgtgagc agcgtgacgg
     2581 gcaaggtgag gaccaggagg atggggcgca cgacggcgtt gatcagcccg agcacgagcc
     2641 cggcgccgag cgccgcccag acgctgctca ccctgatgcc gggcagcagc atcgccacga
     2701 cgtagattgc cgcggcgttc gcgaggaccc ggaaaacgaa gcccaccccg ccgagtgtac
     2761 tccgactgct cccgacgcca cggtcgcgat tcggacgtt
//
LOCUS       DATIAZ010000227         2639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1507774, whole genome shotgun sequence.
ACCESSION   DATIAZ010000227 DATIAZ010000000
VERSION     DATIAZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507774"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VLF19_08035"
     CDS             <1..591
                     /locus_tag="VLF19_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93240.1"
                     /translation="VVASLRETFSVEKFEAFLAERAADHAAREERRAERARLLDVVLP
                     DLRTQAAKLVKRIAMVEDDSLVADLQAAYKEIKGQIEAGEARVLDLEMYETQAKDQTE
                     AIEKLREVWGSWSGAFDAVSGEMVKARSILKKILVDRIKVTPTDDGWTFYGTSDYEAS
                     CRAASGGRRSRRSTTRPASRPAASPGRTSARSARST"
     gene            759..2570
                     /locus_tag="VLF19_08040"
     CDS             759..2570
                     /locus_tag="VLF19_08040"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HSE93241.1"
                     /translation="MYLRRTARRVGDKTYQNYLLVESVATPKGPRQRVICSLGALAPA
                     PKDEWLSTARRLHAALAGQTTLVPDATVDALAGRARLPRRTPPAAGPGVTIDPERVTF
                     EDEREAGPVHVGHQMWRLLHLDPILADAGLSRRAQLLTEVMTLNRLVRPAAEHAMPDW
                     IRRTALADILGTDFTALSDEALYRNLDRLHPQRARIEQALAARERTLFNLDDTIYLYD
                     LTSTYFEGQCPRNPQALRGYSRDHRPDCKQVVIGLVLDRDGFPKAHEVFDGNRHDQTT
                     VEDMLTRLEERTGRRGGATVVVDRGMAFAKNLAQIRARGHHYLVAARYPERGDHQEAF
                     AADADWTEVVRVPSPRNPGQKKTRVIVKRQTIGDEVHILCRSEARIEKDRAIRDKHEQ
                     RLLADLAKLQRRVAQGRLRDPAKVQEAIGRLKERYSRVARYYTITCDAATAAVSWTEQ
                     AEKKTAASALDGTYLLKTDRQDLADDEVWRLYILLTRVEAAFRALKSPLMERPIFHHL
                     EHRVQTHIFLCVLAYHLLVAIEKRCLDQGRHTSWATLREQLSTHQVATVVLPAIDGRI
                     LKIRKASTPDDVHQEIYRLLQVPPEVMKPVKTWTASR"
BASE COUNT          472 a          921 c          880 g          366 t
ORIGIN      
        1 gtcgtcgcct cgctacggga gacattctcg gtcgagaagt tcgaggcctt cctggcggag
       61 cgggccgccg accacgcggc gcgcgaggag cgacgtgcgg aacgggcgcg gctgctcgac
      121 gtcgtgctgc cggacctccg cacgcaggcg gcgaagctcg tgaagcggat cgccatggtc
      181 gaggacgact ccctggtcgc cgacctccag gcagcctaca aggagatcaa gggccagatc
      241 gaggcgggcg aggcccgcgt gctcgacctg gagatgtacg agacgcaggc caaggaccag
      301 acggaggcca tcgagaagct gcgcgaggtg tggggctcct ggtcgggcgc cttcgacgcg
      361 gtcagcggcg agatggtcaa ggcccgctcg atcctcaaga agatcctggt cgaccggatc
      421 aaggtcacgc cgaccgacga cggctggacc ttctacggca ccagcgacta cgaggcgtcc
      481 tgtcgggcgg cgtccggggg ccggcgttct cggcggtcca ccacaagacc ggcatcgcga
      541 ccggcggcct cacccggacg aacatcagcg aggagcgcgc gttcgaccta gcccgacttc
      601 cacggtggcc gacgacgaaa cggctgattc cggccgtcgc cgcaccgtaa gtgcgctgga
      661 tgtcgtgagt gccccacgcg tcgggcgaaa atagtgacta agtctctggc cgattttact
      721 tgacgagtct cgctcgatag tgacacgctg ccggcggtat gtatctccgt cgcaccgccc
      781 ggcgggtcgg cgacaagacc taccagaact atctcctcgt cgaatcggtt gcgaccccga
      841 aggggccgcg ccagcgcgtc atttgctcgc tcggcgcgct ggcaccggcg ccgaaggacg
      901 agtggctcag caccgcacgg cggcttcatg ccgccctcgc ggggcagacg accctcgtgc
      961 cggatgccac ggtcgacgcg ctcgccggcc gcgcgcgtct cccgcgccgc accccgccgg
     1021 ccgcgggccc gggcgtcacc atcgaccccg agcgcgtgac gttcgaagac gaacgggaag
     1081 cgggcccggt gcacgtgggg catcagatgt ggcgcctgct gcacctggac ccgatcctgg
     1141 ctgacgccgg gttgagtcgg cgcgcgcagc tcttgaccga agtgatgacg ctgaaccggt
     1201 tggtgcggcc ggcggcagaa cacgccatgc ccgactggat ccggcgcacg gcgctggccg
     1261 acatcctcgg cacggacttc accgccctca gcgacgaagc gctgtatcgc aacctcgacc
     1321 ggttgcatcc acagcgcgcc cggatcgagc aggcgctggc ggcccgcgag cgcacgctgt
     1381 tcaacctgga cgacaccatc tacctctacg acctgacctc gacgtacttc gaagggcagt
     1441 gcccgcgcaa cccgcaggcc ctgcgcggct attcgcgcga tcaccgcccg gactgcaagc
     1501 aggtggtgat cgggctggtc ctggaccggg acggcttccc caaagcgcac gaagtcttcg
     1561 atggcaatcg gcacgatcag accaccgtgg aggacatgct cacgcgtctg gaggaacgca
     1621 cggggcgccg gggcggtgcg acggtcgtgg tggaccgggg catggccttc gcgaagaacc
     1681 tggcgcagat ccgggcgcgc ggccatcact acctcgtagc ggcgcgctat ccagaacgcg
     1741 gggaccacca ggaggcgttc gccgccgacg cggactggac cgaagtggtc cgcgtgccct
     1801 cgccgcgcaa tccggggcag aagaagacgc gggtgatcgt caagcgacag acgatcggcg
     1861 acgaggtgca catcctctgc cgcagtgagg cgcgcatcga gaaggatcga gcgatccgcg
     1921 acaagcacga gcagcggctc ttggccgacc tcgccaaact ccagcggcgc gtcgcgcagg
     1981 gccgcctgcg cgaccccgcc aaggtccagg aggcgatcgg ccggctcaag gagcgctact
     2041 cgcgggtcgc gcgctactac accatcacct gcgacgcagc gacggccgcg gtgagctgga
     2101 ccgagcaggc agagaaaaag accgcggcca gcgcccttga cggcacctac ttgctcaaga
     2161 ccgatcggca ggacctcgcc gacgacgagg tctggcgcct ctacatcctg ctcacgcgcg
     2221 tcgaggccgc gttccgcgcc ctgaagagcc cactcatgga gcgcccgatc ttccaccacc
     2281 tcgagcaccg cgtgcagacc cacatcttcc tctgcgtgct cgcctaccac ctgctcgtcg
     2341 ccatcgagaa gcgctgtctc gaccaagggc gccacacctc gtgggccacg ctgagggagc
     2401 agctttcaac ccatcaggtg gcgaccgtgg tgctacccgc gatcgacggc cgcatcctga
     2461 agatccgcaa ggcgtccacg ccggacgacg tgcaccagga gatctatcgc ctcctgcagg
     2521 tgccgcccga ggtcatgaag cccgtgaaga cctggacggc cagtcgatag tgacgcaatg
     2581 cccacgcaac ctcccgaaat tccagcattc cactcctcag accgtggaag tcgggctag
//
LOCUS       DATIAZ010000228         3702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_981470, whole genome shotgun sequence.
ACCESSION   DATIAZ010000228 DATIAZ010000000
VERSION     DATIAZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981470"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VLF19_08045"
     CDS             complement(<1..1190)
                     /locus_tag="VLF19_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HSE93242.1"
                     /translation="MTDLEVLDRIQKRLLWLSTWMVHHANTARASADGLKVGGHQASS
                     ASVVSLLTALYFKTLGPGDIVAVKAHASPAFYAAQFLRGRLDAAALQGLRDLGGLQAY
                     PSRRKNPEVVDISTGSMGLGAVQATFGALATRYLVDHGGPRVPERVIVIVGDAELDEG
                     NVWEALAEEAVGRLGDVLWIVDVNRQSLDRVVPDARARQLPALFEAFGWRVIELRWGR
                     RLGALFSRPGGAALRARLTAMPYAEYQNLLRLPVAAVRKTLAAGDEAAVIDRRLADVP
                     DDALPALVADLGGHDLAAIIDALDEARRERGRPTVILAHTIKGWGLPLAADPLNHTAL
                     LTSTQIETLRASLGVAAGAEWEGFGPDSEEAAWIQRLPPLFAPPPPAPPPSVPAEIAE
                     TYPD"
     gene            complement(1280..2488)
                     /locus_tag="VLF19_08050"
     CDS             complement(1280..2488)
                     /locus_tag="VLF19_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE93243.1"
                     /translation="MTPAWRQLTILSLAELLALSLWFSASAVLPALTREWQLGDAGRA
                     GLTNSVQLGFIVGTLLSALGNLPDVLSPRRLMVATALLGAGANAAIAVWADGPATTLV
                     LRFLTGVAMAGAYPPAMKIMATWFREGRGLAIGILVGALTVGSATPHLIRGVTDLPWR
                     QVLLAASALALGAAVIVHRWVAEGPHRFPSARFDVRMAGAVFRDRGARLACFGYFGHM
                     WELYAMWAWIGVFFGASLAARGGGHYAGLNASAAAFVVIAAGALGCWLGGLVADRWGR
                     TTLTMAAMALSGLCAVLIGLTFGGPPALTLAVALLWGVAVIADSAQFSTAVTELSPPA
                     YVGTALTMQTCVGFAITIASIWLIPVAVAHVGWRWAFASLAIGPALGVLAMARLRALP
                     DSLRLAGGRR"
     gene            2632..3450
                     /locus_tag="VLF19_08055"
     CDS             2632..3450
                     /locus_tag="VLF19_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HSE93244.1"
                     /translation="MSRARPGSARRASAALPDLSRYGTLAEYNRKRHFGVTPEPPGRR
                     RTPDRTASPREFVVQKHRATRLHYDFRLEHRGVMLSWAVPKGPSLDPTVKRLAMETEP
                     HPMDYNQFEGVIPEGEYGGGTVMIWDRGVWAPVADGRLGDESDVDRQLAKGELKFVLL
                     GDKLAGSWVLVRTRGERQWLLIKHRDEHDSTEDVTVTRPQSVVSDRSMAAIARAAGAT
                     PRQLAQAMGADPTEAPAAAPAAPAPRSRARGEPQPAATRPAAPSPRPRRPRRPA"
     gene            complement(3395..>3702)
                     /locus_tag="VLF19_08060"
     CDS             complement(3395..>3702)
                     /locus_tag="VLF19_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93245.1"
                     /translation="VGPLVWRLVQDRREERAQWLEAQIRSAVREALGGESLLSVRVEP
                     RTALHAGRVMLSAPHGWESLIEPVWKKALASTPADFELVIMPGGGAVGAEATERRAA"
BASE COUNT          559 a         1359 c         1365 g          419 t
ORIGIN      
        1 tgatcggggt acgtctcggc gatctccgcc ggcaccgagg gtggcggcgc ggggggcgga
       61 ggggcgaaca gcggcggcag ccgctggatc cacgccgctt cctcgctgtc cgggccgaag
      121 ccctcccact cggcccccgc cgccacgccg agcgacgccc ggagcgtctc gatctgggtc
      181 gacgtgagca gcgcggtatg gttcagcgga tcggcggcga gtggcagccc ccatcccttg
      241 atcgtgtgcg ccaggatcac ggtcggacgg ccgcgctcgc gccgtgcttc gtccagcgcg
      301 tcgatgatgg ccgcgaggtc gtgaccgccg agatcggcga cgagcgcggg cagtgcgtcg
      361 tccggcacgt cggcgagccg tcgatcgatc accgcggcct cgtcccccgc agcgagcgtc
      421 ttgcgcaccg ccgccaccgg cagacgcagc aggttctggt attcggcgta gggcatcgcc
      481 gtgaggcgcg cccgtagcgc ggcgccgccc gggcgcgaga acagcgctcc gaggcggcgg
      541 ccccagcgca gctcgatcac ccgccagcca aaagcctcga agagggcggg gagctgccgg
      601 gcgcgcgcat cgggcacgac gcggtcgaga ctctggcggt tgacgtccac gatccagagc
      661 acgtcgccca gtctgccgac cgcctcctcg gcgagggctt cccagacgtt gccctcgtcc
      721 agttcggcgt cgcccacgat gacgatcacg cgctccggaa cacgcggtcc gccgtgatcg
      781 acaaggtagc gggtggcgag cgcgccgaac gtcgcctgca cggcgccgag ccccatggag
      841 ccggtcgaga tgtccaccac ctcggggttc ttgcggcggc tcgggtaggc ctgcagtccg
      901 ccgagatcgc gcaggccctg gagtgccgcg gcgtcgagac ggccgcgcag gaactgggcc
      961 gcgtagaacg cgggggaggc gtgggccttc actgcgacga tgtcgccggg cccgagcgtc
     1021 ttgaagtaga gggccgtgag caggctcacc accgacgcgg acgaggcctg atggccgccg
     1081 accttgaggc catccgcgct cgcgcgcgcg gtgttggcgt ggtgaaccat ccaggtcgag
     1141 agccagagca gtctcttctg gatgcggtcg agcacctcga ggtcggtcat cggcctcgga
     1201 ctctacccgc gcgaccgcga cgccgcaacg gcgcttgtgc gcggcgcccc ctcgggcccg
     1261 cctcgacatc accagcccgt caccgccggc ccccggccag cctcaatgag tcgggcagcg
     1321 cccgcagccg ggccatggcc aacacgccga gcgcggggcc gatcgccagc gaggcgaagg
     1381 cccagcgcca gccgacgtgc gcgacggcga cggggatcag ccagatcgag gcgatggtga
     1441 tggcgaagcc gacgcacgtc tgcatggtca gcgccgtccc gacgtacgcc gggggcgaca
     1501 gctccgtcac cgccgtcgag aactgcgccg agtcggcgat cacggcgacc ccccagagaa
     1561 gggcgacggc cagcgtgagc gccggcgggc ccccgaaggt cagcccgatg aggacggcgc
     1621 agagcccgct cagcgccatc gccgccatcg tgagcgtcgt ccggccccac cggtcggcga
     1681 cgagaccgcc gagccagcag ccgagcgcgc cggcggcgat gacgacgaag gctgcggcgc
     1741 tggcgttgag ccccgcgtag tggccgccac cgcgcgctgc gaggctcgcg ccgaagaaga
     1801 cgccgatcca cgcccacatc gcgtagagtt cccacatgtg cccgaagtag ccgaagcacg
     1861 ccagccgcgc gccgcgatcg cggaacacgg cgccggccat gcgcacgtcg aagcgcgcgg
     1921 aggggaagcg atgcgggccc tcggcgaccc agcgatgcac gatgacggcc gcgccgagcg
     1981 cgagggccga ggccgcgagc agcacctggc gccacggcag gtccgtgacg ccgcggatca
     2041 ggtgcggggt ggccgacccc acggtcagcg cgccgacgag gataccgatg gccaggccgc
     2101 ggccttcgcg gaaccacgtg gccatgatct tcatcgccgg cggataggcg ccggccatgg
     2161 ccaccccggt caggaagcgc aggacgaggg tcgtcgccgg tccgtcggcc cagaccgcga
     2221 tcgcggcgtt cgcgccggcg ccgagcagcg cggtggcgac catcaggcgg cgcggcgaca
     2281 gcacgtcggg gaggttgccg agcgcggaga gcagcgtgcc cacgatgaat ccgagctgca
     2341 cggaattggt gagaccggcg cgcccggcgt cgccgagctg ccactcgcgc gtgagggcgg
     2401 gcaggacggc ggaggcgctg aaccacagcg agagcgcaag caactcggcc agcgacagga
     2461 tggtgagctg ccgccaggcg ggggtcacgg cggcgagtgt agaggtgggg acacgtgcgg
     2521 tgcaacgatg gcgggtgatg gccggcgccg ccgatcggct cgaggaacga ccgctggcgg
     2581 cgcgcgtccc gcggcctggc tcggccaggg ccgcatcgtg agcctggccc gatgagccgg
     2641 gctcgcccgg gatcagcccg tcgcgcaagc gcggcgctgc ccgacctgag ccgctacggc
     2701 acgctcgcgg agtacaaccg caagcgccac ttcggcgtca cgccggagcc gccgggccgc
     2761 cggcgcacgc cggacaggac ggcgagtccg cgcgaattcg tcgtccagaa gcaccgcgcc
     2821 acccgcctgc actacgactt ccgtctcgag caccggggcg tcatgctctc gtgggctgtg
     2881 cccaagggtc cctcgctcga tcccacggtc aagcggctgg cgatggagac cgagcctcat
     2941 ccgatggact acaaccagtt cgagggcgtc atccccgaag gggagtacgg cggcggcacc
     3001 gtgatgatct gggaccgcgg cgtctgggcg ccggtggccg acggccggct cggcgacgag
     3061 tcggacgtgg accgtcagct cgccaaaggg gagctgaagt tcgtcctcct gggagacaag
     3121 ctcgccggct cgtgggtgct cgtccgcacg cgcggcgagc gccagtggct cctgatcaag
     3181 caccgcgacg agcacgactc gaccgaggac gtgaccgtca cccggccgca gtcggtggtc
     3241 tccgaccgct cgatggccgc catcgcccgc gccgccggcg cgacgccgcg tcagctcgcg
     3301 caggcaatgg gagcggatcc aacggaagct ccggcggccg cgcccgccgc gccggccccg
     3361 cgctctcgag ctcggggcga gccgcagccg gcagctacgc ggcccgccgc tccgtcgcct
     3421 cggccccgac ggccccgccg cccggcatga tcacgagctc gaagtccgcc ggcgtcgacg
     3481 ccagcgcctt cttccacacc ggctcgatga gcgactccca gccgtgcggc gccgagagca
     3541 tcacgcggcc cgcgtgcagc gccgtgcgcg gctcgacgcg caccgagagc agcgactcgc
     3601 cgccgagagc ctcgcggacg gccgaccgga tctgcgcctc gagccactgc gcacgctcct
     3661 cgcgccggtc ctgcaccagg cgccagacga gcggtcccac ga
//
LOCUS       DATIAZ010000229        19579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_589375, whole genome shotgun sequence.
ACCESSION   DATIAZ010000229 DATIAZ010000000
VERSION     DATIAZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 19579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19579
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589375"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="VLF19_08065"
     CDS             complement(<1..1670)
                     /locus_tag="VLF19_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSE93246.1"
                     /translation="MPAPSAVAVAPRRPAAAGGTGVVWLLAAGLIAALVAAPLAGVLV
                     SLLAPRAEVWGHLLRTQLAELMVNTVLLLLGVGAGTLALGTSLAWLVVAFRFPGRALF
                     ESALVLPLAVPAYVIGFAFLGVFDYAGPVQTALRAWLGPGAWLPEPRSYGGVALVMTL
                     VFYPYVYLLARTAFREQGAATLETARSLGMTRGRAFVTLALPMARPALVAGVSLAMME
                     ALADFGTVATLGYRTLTEAVYRVWHGMFDRVAASQLAGLLMLFAFLLLALERALRGRA
                     RFVQSQRRGPGIVPVPLAGWRAWTATLACGAVLGLAFVVPVGRLVLWAAETAATSGAL
                     VGFPSLLGNTVWLAGLAAATTTALALALAYATRLRPTPGVRLAAQFAGMGYALPGAVI
                     AVGVLVPVALVDHALAALAEAALGRPAAMVLTGSAVALLFAYVVRFLAVGLQTVDAGL
                     GRIAPNLDNAARSLGAGTAGVVRRVHLPLVRGSLLTAAVLVFVETMKEMPATLLLRPF
                     GLETLAVAVWERTAESMWAEAALPALAIVAAGLLPVWLALRLSERGARR"
     gene            complement(1675..2697)
                     /locus_tag="VLF19_08070"
     CDS             complement(1675..2697)
                     /locus_tag="VLF19_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE93247.1"
                     /translation="MGHEIRTLATLLALDATILATWAAPGPAAAQGPEVVVYSARHYG
                     QEAAFGAFTRKTGIQLKILNGSTGELFERLKAEGARTPGDVLLTVDAGNLWNAARAGL
                     LSPVDSPELAANIPAHLRDPEHRWVGLTVRARTIVYNTKKVKPDELSTYEALGDPKWK
                     GRLCLRTSSYIYNQSLLATMIKRHGEPRTEQIVRGWVANAPVLINGDTNIVQAVAAGQ
                     CDVALVNHYYLARVLAKAPDTPVGLVWANQATTGTHVNVSGAGVTAHARHRANAIKFL
                     EFLSTPEAQQMFADLSHEFPANPQAGVNALVTRWGAFKRDDVNVAAAGEFQAAAVRLA
                     DRAGYK"
     gene            2811..3539
                     /locus_tag="VLF19_08075"
     CDS             2811..3539
                     /locus_tag="VLF19_08075"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HSE93248.1"
                     /translation="MGKTSSLKSGAPAATTASVQDYLAAIYDLGGSGRPVIGARLAKH
                     MGISAPAVTEQVQRLTRGGYVKVGRGKALALTPKGREIAEVMARRHRLLERWLTDKLG
                     LDWTDAHEEAHRLEHAISPRVEERLAEMLGMPSTCPHGNPIPGMASPRRVEPFPLAQA
                     PEGANVVVERITEEAEADKKLLEHLWRNDVRPGRRLRIVEVAPWAGTITAASDDRTIA
                     LGLPAAAKIWVYDPRRTEKDGERA"
     gene            3536..3871
                     /locus_tag="VLF19_08080"
     CDS             3536..3871
                     /locus_tag="VLF19_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93249.1"
                     /translation="MKALGGAFVVVAFFYVFYAGVTTLWSYLQVASVVDRAWEDQGRN
                     GAAAVRTAIVKGASEAGVPLEVRFVQVGEDERIMAVAVRWSFPAVSFRGDTFVEIPLS
                     LQRSYEKRP"
     gene            complement(3868..4830)
                     /locus_tag="VLF19_08085"
     CDS             complement(3868..4830)
                     /locus_tag="VLF19_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93250.1"
                     /translation="MTVALVALLLGLPAAAFTLWPLLSGRRARALLAVPPDPREPLLE
                     QRRHALLTLRELDFEHEAGHVSDDDYADLRARYEAETAQILTELDRLGPEPVVKTAVP
                     RASSTAPAPRGFRHPLALAASAVALVVFGVVLGVGIVRYSEPDATAGMPGPPGMPGAG
                     GMSGPGGMSPAGGSAPMAETPGEAPAGGGAPRPVSPEMLRGMLEAARSSLGAGRYGEA
                     IAAYQAVLKRDPNNVDAMTHLGLIVAIGGHADSALQTFDKALAIDPNYAPALLYRGQV
                     LYESKRDAPGAIASWEKFLKLVPAGQDHERVKALIAEARKPVKN"
     gene            complement(4827..5300)
                     /locus_tag="VLF19_08090"
     CDS             complement(4827..5300)
                     /locus_tag="VLF19_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HSE93251.1"
                     /translation="MIRLATGVLAALSLIGTVTPGLAQQPARHVDEQEAHEIAARLRC
                     VVCQNLSVADSPSEMASQMRGVIRERLATGQRPEQVVQYFVDKYGEWILLEPRRSGFT
                     LLVWVVPPVAAVVGLAVVAVLLGRWTRRGRAARRAEAPVDPAMAERIRREMEREA"
     gene            5421..5702
                     /locus_tag="VLF19_08095"
     CDS             5421..5702
                     /locus_tag="VLF19_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93252.1"
                     /translation="MPVSVGTGLRVTEPARVRKTERTDVCPAGGGAADRARQWVARAF
                     TRLEDVVYVGLSVLLAGTALGLLATGLIDFVRLLITGTLSSWPCSTACS"
     gene            5675..5977
                     /locus_tag="VLF19_08100"
     CDS             5675..5977
                     /locus_tag="VLF19_08100"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HSE93253.1"
                     /translation="MALLDRVLLILVFVEVLYTVQVSFREHALVPEPFLIVGLIAAIR
                     RVLVLTAELAELTERGEGAFRNAIVEPGLLTALIVALVLSLVLLRRRPPHAVADRA"
     gene            complement(5996..6526)
                     /locus_tag="VLF19_08105"
     CDS             complement(5996..6526)
                     /locus_tag="VLF19_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSE93254.1"
                     /translation="MKRYLVWLIPLSAVPVLALLAYGFRTNPRDVPSPLVGRPAAPFA
                     LTTFAGEPVSLEALRGKVVVLNFWASWCYPACYEEAPVLERGWQEWRERGVLVLGVDI
                     QDTTEKARKFIDDFRLTFPNAPDAGGKVSIDYGVYGVPETFFIDRRGVIRVKHVGAVT
                     EAVFRTTVERLLAEPP"
     gene            complement(6523..8469)
                     /locus_tag="VLF19_08110"
     CDS             complement(6523..8469)
                     /locus_tag="VLF19_08110"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HSE93255.1"
                     /translation="MTPELGQGALVVAFALALWGMVASAAAAGTARPALVESAQHAAL
                     GVFALVTFALGLLVYAFLTFDFSVRYVAGNTNLGTPYYYRVTAVWGALEGSLILWGWM
                     LALYTLVLIVRQRVRARELYPWALAVMLGVMAFFLSVMTIAAPPFTRLTPVPADGRGL
                     NPLLEDTGMITHPVALYLGFTGFTVPFAFAIAALATGRVGDTWLTLTRRWTITAWYFL
                     SLGLLIGGWWSYHVLGWGGYWAWDPVENAAFMPWLTGTAFLHSVMIQERRRMLKLWNV
                     TLVILTFGLTLFGTFLTRSGVIGSVHAFSQGSVGVFFLGILAVVLLLALGLLAWRWDA
                     LRAQGELDSVVSRESAFLLNNVMLVAATFTVFFGTVFPLLSEAVRGVKVSVGAPFFNQ
                     VNVPLFLGLVFLMGVGPLIAWRRASLGNLRRNFVGPVAVGVVAAAVFFALGVRSTLAL
                     VSLALTIFVAATIALDLGRATRARQRMGERLLPALGELLTRHNRRYGGFIVHLGILVI
                     AVGVTGSQAWSVQTETTLKVGEAAQLAGYQVRFDGLGTVEESNHFKVVGRFTVQNGRT
                     RPAVMQPSKKFYPQEQSPLAGVDYWLGLTEDLYLVLGDFTRDGSQATVKIQVNRLVSW
                     IWIGGLVLTLGAALAVMPERRKAA"
     gene            complement(8466..8900)
                     /locus_tag="VLF19_08115"
     CDS             complement(8466..8900)
                     /locus_tag="VLF19_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HSE93256.1"
                     /translation="MKTKFVAGGLVIAAAIVYMIYAGVTQSAVYFVTPSELRAAPVTN
                     KAYRLGGLVTPGSLRWEPRTLDLSFTLTDGKASIPVRHKGTAPDLFGEGRGAVVEGQW
                     TPEGHFKASLILAKHSEEYKAPHDASDAGYRELLKSLRKPGS"
     gene            complement(8902..9192)
                     /locus_tag="VLF19_08120"
     CDS             complement(8902..9192)
                     /locus_tag="VLF19_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93257.1"
                     /translation="MPDNWGYVFAAYGLAAFVLAAYWRHLVRLDRALSALAARADAPR
                     ARTKDRTTTEPTAGAAAGLGGSGSIDREAERIRAAAGSGHPRGEPASRPPGQ"
     gene            complement(9192..9848)
                     /gene="ccsA"
                     /locus_tag="VLF19_08125"
     CDS             complement(9192..9848)
                     /gene="ccsA"
                     /locus_tag="VLF19_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSE93258.1"
                     /translation="MTTATRTFGWLAVVGLAAGLVAAFGVAPREATQGNVQRIMYVHV
                     PAVWVAYLAFLVVFGTSLGYLWTRSRGADRLAAASTEVGVVFLGINIATGSIWGKPTW
                     GTWWTWDARLTSVSVVFLMYLGYLLLRRMTDDPERSARFAAVLGVLAAPSIYLVHTSV
                     YWWRTLHQPPSLMKAGNATMPAVFWSAILINFVALGLLAAYFVAKRVGLLRREQEVLA
                     "
     gene            complement(9845..10519)
                     /locus_tag="VLF19_08130"
     CDS             complement(9845..10519)
                     /locus_tag="VLF19_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HSE93259.1"
                     /translation="MSYARRAWIVLWKDLVSERRSKETLNALFFFSLLLLFLFQFALG
                     DRERLLAVLPGLLWLGIVLSGLVGLGRAFLAERENDCWEGLLLAPGDKSAIYLGKVAG
                     NVLLMLLVEAILVALFAVFFDVDVTAHPGPLALVLALGTVGFAAVGTLFAAMTAHVRA
                     RELLFPVLLLPVQVPVLLGAVKATEAVLAGEPLGAVAHWLKLLVAADVIYVTVGLLTF
                     DAILEP"
     gene            complement(10516..11232)
                     /gene="ccmA"
                     /locus_tag="VLF19_08135"
     CDS             complement(10516..11232)
                     /gene="ccmA"
                     /locus_tag="VLF19_08135"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HSE93260.1"
                     /translation="MTAEPLVHVVGLRKAFGGHFVLDDVALDVRPGEAVALLGPNGAG
                     KTTLLRILATLARPTRGIARVAGHDCTREAERVRALVALVGHGTWVYDDLTALENLRF
                     WATLGGQAADADVLRTALASVDLERLADERARTFSLGMKRRLALARLVLTRPRVLLLD
                     EPFAALDQAAGKWLEARLTAFKAAGGAVLLTTHSFGRGLGVADRVAILAGGRVVLDTP
                     SAALTAEDLRKLYALHVEDA"
     gene            complement(11405..12253)
                     /gene="htpX"
                     /locus_tag="VLF19_08140"
     CDS             complement(11405..12253)
                     /gene="htpX"
                     /locus_tag="VLF19_08140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSE93261.1"
                     /translation="MSNVFKTAMLLAILTAMLVLIGGAIGGQQGMVMAFVMALAMNFF
                     SYWFSDRIVLAMYGAKPVDEAQAPGLYAMVRRLTSRAGIPMPRVYVIPTDTPNAFATG
                     RNPQHAAVAVTEGITRLLDEEELEGVLAHELAHVKNRDVLIATIAATMAGAITYLAHM
                     AQWAAMFGMHRGDNEEGGTNPFAAILMAILAPIAALLVQMAVSRSREYQADATGARLA
                     GKTWGLAKALEKLHMAQQVVPMAANPATAHLFIVNPLSGRALMNLFSTHPPMEERIAR
                     LRSMRV"
     gene            12445..12954
                     /locus_tag="VLF19_08145"
     CDS             12445..12954
                     /locus_tag="VLF19_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HSE93262.1"
                     /translation="MIIRLSEIEDDGLVVANAGEFVAPFSDRSWRLDGLRLHVARDGE
                     DVVVTGRIDATVPVVCSRCLEEFRADVHPVVDARYVPKPTAGHDVELGADDLDLDFYE
                     NDELNLAALVETETTLALPMKPLCRADCRGLCPVCGVNRNVTPCTCERRAPDPRLSAL
                     RDLAARLNH"
     gene            12967..13149
                     /gene="rpmF"
                     /locus_tag="VLF19_08150"
     CDS             12967..13149
                     /gene="rpmF"
                     /locus_tag="VLF19_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSE93263.1"
                     /translation="MPLPKRRHSRARGRKRRTHYKLGAPTNSVCPQCRESKLPHRVCP
                     HCGYYKGREIIAVEGA"
     gene            13196..14218
                     /gene="plsX"
                     /locus_tag="VLF19_08155"
     CDS             13196..14218
                     /gene="plsX"
                     /locus_tag="VLF19_08155"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSE93264.1"
                     /translation="MKIAVDAMGGDYGPAVVVEGAVSAAREHRASVILVGDKAAVERE
                     VVRLQAEPLGLEIRHASQVVGMGESPSQALRRKRDSSLRVAAQLVKDGEAAAFVSAGN
                     TGAAMAIAMFVIGVLPGVDRPAIAAVLPNRRRFTVLLDVGANVDPKAWHLLQFAVMGH
                     VYARDILGLDNPRVGLLSVGEEEGKGSQLTREAYDQLKDSSLNFIGNVEGRDIYNGRC
                     DVVVTDGFTGNVALKISESLVETLGEMIKEELTRDVRSKLGAALALPAFHRFKRRVDY
                     TEMGGAPLLGIDGAAIVCHGASPVKAIKNAVRVAGEWAKAGVNEHIRAALEAEVTRGV
                     REGGRE"
     gene            14215..15195
                     /locus_tag="VLF19_08160"
     CDS             14215..15195
                     /locus_tag="VLF19_08160"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSE93265.1"
                     /translation="MTRAKIIGVGAYAPKRILTNADLEKMVDTNDAWIVQRTGIRERH
                     IADEGEATSDLALRAAQQALERANLVPEDIDFIVVGTTTPDMFFPTTGNILQHRLGCR
                     RAGSVDLLAACAGSVYSLAVGAKFIEAGKYRRVLCVGAETLSRITDFTDRGTCILLAD
                     AAGAVVLEAGEGEAGIIDADLYSDGQYWELLYMPGGGARHPATRETVDARMHYAKMKG
                     NDVFKVAVRMFVECTETILGRHGFTAADVNLFIPHQANLRIIEAAVKRVGLPMEKVFI
                     NVDRYGNTGAASIYVALEEAVAAGRVKRGDLVLLAAFGGGFAWGAVLLRW"
     gene            15275..16201
                     /gene="fabD"
                     /locus_tag="VLF19_08165"
     CDS             15275..16201
                     /gene="fabD"
                     /locus_tag="VLF19_08165"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSE93266.1"
                     /translation="MIAFLFPGQGSQAVGMGRAFYETSPAAKTVFEEANDALGFDLAR
                     LAFEGPEAALTLTANTQPAVLTVSIAAATVAAERGLRPAIAAGHSLGEYSALVVAGAL
                     AFRDAVRVVRRRGEFMQDAVPVGTGAMAAVMGMDLTAVEQVCVEAGAGEVVEVANVNS
                     PQQIVIAGHKTAVERAVSLAAARGGRKSVVLPVSAPFHCALMRPAAERLARELDGVLV
                     TDPRIPVVRNVDAGVTRVAAEVKPALLEQVARTVRWTACVQRLADEGATRFVEVGPGR
                     VLTGLLRRIVDGARGVAVETPDDLDRALEDAT"
     gene            16198..16947
                     /gene="fabG"
                     /locus_tag="VLF19_08170"
     CDS             16198..16947
                     /gene="fabG"
                     /locus_tag="VLF19_08170"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HSE93267.1"
                     /translation="MSTAPLAGRVAIVTGGSRGIGAAVAALLVEHGAAVVVSGRDPDR
                     LERAVKELEGGGGTALGVVADAASREDAERLADTARQRFGRIDVLVNNAGVTRDSLLL
                     RMKDEDWDRVMDVNLRGAFVLTRAVAKVMVRQKDGRIINVASTAGAMGNAGQVNYSAA
                     KAGLIGFTKAAARELAHWRILVNAVAPGLIETDMTATVPEAARQALLAQVPLGRIGTA
                     REVAEVVRFLAGDGAAYVTGQVFHVNGGLYM"
     gene            16975..17211
                     /gene="acpP"
                     /locus_tag="VLF19_08175"
     CDS             16975..17211
                     /gene="acpP"
                     /locus_tag="VLF19_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSE93268.1"
                     /translation="MAKSVEDKVKEIIVEQLGVEEDDVTPTAKFIEDLGADSLDTVEL
                     VMALEEHFDIQIPDEDAEKIVTVGDAIQYIKDNS"
     gene            17208..18482
                     /gene="fabF"
                     /locus_tag="VLF19_08180"
     CDS             17208..18482
                     /gene="fabF"
                     /locus_tag="VLF19_08180"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSE93269.1"
                     /translation="MTVDRRRVVVTGLGALTPLGNTAEEFWTGLVQGRSGIGPITKFD
                     AGEKTASGEYRYETRIAGEVRNFDPLKFVDKKDARRLDPYLQYAIASSAMAVEDAGLD
                     VDAIDRDRFGVLIGSGIGGISTLLEGERVRLEKGPERVSPFIIPMLIVNMASGLVSMR
                     FGAKGPNSAVVTACATGNHAIGDAFRLIQRGDADLMIAGGAEAIIVPLTIAGFCAMKA
                     MSTRNDAPEKASRPFDADRDGFVPSEGAGIVLLEALEHARRRDARIYGEIVGYGMTAD
                     AHHMTAPDPEGDGAFRAMAAALRDAGLEAGRVGYINAHGTSTPYNDKFETVAIKRLFG
                     DHTRRLAVSSTKSMTGHLLGAAGGVEAIATVLALNHGLLPPTVNYETPDPECDLDYVP
                     NQARKQDVEVALSNAFGFGGTNATLVFTKFRP"
     gene            18483..18935
                     /locus_tag="VLF19_08185"
     CDS             18483..18935
                     /locus_tag="VLF19_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HSE93270.1"
                     /translation="MSGVGDLERRLGHRFRDGALLERALTHASYANEHPPRDHNEALA
                     FVGDAALGLVVAERLFAADPQAPVGVLTPRRAEIVSGAALARWAAALDLGVLLRLGRG
                     EDQTGGRTRESVLATTLEAVLGAVYLEAGLDGVRPVVARLAAASPPSA"
     gene            18948..>19579
                     /locus_tag="VLF19_08190"
     CDS             18948..>19579
                     /locus_tag="VLF19_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSE93271.1"
                     /translation="MRLWPSQRRPVVAAVPPADLAVTAELVRGAIEDVFAARQHGVGS
                     AGVVFRGELRLPPARAVDTLQRRLRPFGFTPFLRAEGGDVVLQAVPVVEPVERPRARV
                     NVLLFVLTCASTLAAGAFFSGSPTFDAFRGSLAGTFLVAGLPFAATLLAILGVHEFGH
                     YFTARHYRASVSLPYFIPAPPPLFLFGTLGAIIRMRSPAPDRNSLFDIAAA"
BASE COUNT         2985 a         6913 c         7118 g         2563 t
ORIGIN      
        1 cgtcgcgccc ctcgttcact cagccggagc gcgagccaga cgggcagcag cccggccgcc
       61 acgatggcca gcgccgggag cgccgcctcg gcccacatcg actcggccgt acgctcccac
      121 acggccacgg ccagggtctc gagcccgaac gggcgcagga gcagggtggc cggcatctcc
      181 ttcatcgtct ccacgaagac caggaccgcc gccgtcagca ggctgccccg cacgaggggc
      241 aggtggacgc gacggaccac gcctgcggtg ccggcgccga gcgaccgcgc cgcgttgtcg
      301 aggttcggcg cgatccggcc cagcccggcg tccaccgtct ggagcccgac ggcgaggaac
      361 cggacgacgt aggcgaagag cagcgccacc gccgatccgg tgaggaccat cgccgcgggg
      421 cgtcccagcg ccgcctcggc gagcgccgcc agcgcgtgat cgacgagcgc gaccggcacc
      481 agcacgccga cggcgatgac ggcgcccggg agcgcgtaac ccatgcccgc gaactgcgcg
      541 gccaggcgca cgcccggcgt cgggcggagc cgcgtggcgt acgcgagggc cagcgcgagg
      601 gccgtggtcg tcgcggcggc gagcccggcc aaccacaccg tgttgccgag cagggacggg
      661 aagccgacca gggcgccgga ggtcgccgcc gtctcggccg cccagagcac gagacgtccg
      721 acgggcacca cgaacgcgag cccgagcacg gcgccacacg ccagcgtggc cgtccacgcc
      781 cgccatcccg cgagcggcac gggcacgatg ccgggcccgc ggcgctggct ctgcacgaag
      841 cgggcgcgac cgcgcagcgc gcgctcgagg gccaggagca ggaaggcgaa gagcatcagc
      901 agcccggcga gctggctcgc cgccacccga tcgaacatgc cgtgccacac gcggtagacg
      961 gcctcggtga gcgtgcgata gccgagggtc gccacggtgc cgaagtcggc gagcgcctcc
     1021 atcatcgcca gcgacacgcc ggcgacgagc gcgggccggg ccatgggcag cgccagggtg
     1081 acgaaggcgc ggcctcgcgt catgccgagg ctgcgggcgg tctcgagggt ggccgcgccc
     1141 tgttcccgga aggcggtccg cgcgagcagg taaacgtagg ggtagaagac gagcgtcatc
     1201 acgagggcga cgccgccgta cgagcgaggc tcgggcagcc acgcgcccgg gccgagccag
     1261 gcgcgcagcg cggtctgcac cggccccgcg tagtcgaaga cgccgagaaa cgcgaagccg
     1321 atcacgtagg cgggcacggc caggggcagc acgagcgcgc tctcgaacag cgcgcggcca
     1381 gggaagcgga aggcgacgac gagccaggcc aggctcgtgc cgagcgcgag cgtgccggcg
     1441 ccgacgccca ggaggagcag aaccgtgttg accatcagct cggcgagctg cgtccggagc
     1501 agatgacccc acacctcggc acgcggcgcc agcagcgaga ccagcacgcc cgcgaggggc
     1561 gccgccacga gggcggcgat caggccggcg gccaggagcc agacgacgcc ggtcccgccg
     1621 gccgccgccg gccggcgggg cgcgacggcg acggcggagg gtgcgggcat gggcctactt
     1681 gtaaccggcg cgatccgcga ggcggacggc cgcggcctgg aactcgccgg cggcggccac
     1741 gttgacgtcg tcgcgcttga acgcgcccca gcgcgtgacg agagcgttca cgcccgcctg
     1801 gggattcgcg gggaactcgt gcgagaggtc ggcgaacatc tgctgggcct ccggcgtgga
     1861 gagaaactcc aggaacttga tcgcgtttgc acggtgcctg gcgtgcgccg tcacgcccgc
     1921 gcccgacacg ttgacgtggg tgccggtcgt cgcctggttc gcccagacga ggcccaccgg
     1981 ggtgtcgggc gccttggcga ggacccgtgc gaggtagtag tgattcacga gcgccacgtc
     2041 gcactgtcca gcggcgaccg cctggacgat gttggtgtcg ccgttgatga ggaccggcgc
     2101 gttggcgacc cagccccgga cgatctgctc ggtcctgggc tcgccgtgac gcttgatcat
     2161 ggtagccagc agcgactggt tgtagatgta cgacgacgtg cgcaagcaga ggcggccctt
     2221 ccacttcggg tcgcccaagg cctcgtaggt ggacagctcg tccggcttca ccttcttcgt
     2281 gttgtacacg atggtccgcg cgcgcacggt gaggccgacc cagcggtgct cgggatcgcg
     2341 caggtgcgcg ggaatgttcg ccgccagctc gggggagtcc accggcgaca gcagccccgc
     2401 ccgggcggcg ttccagagat tgccggcgtc cacagtgagg aggacgtcgc ccggcgtccg
     2461 cgcgccctcg gccttcaggc gctcgaacag ctccccggtg ctgccgttga ggatcttcaa
     2521 ctggatcccc gtcttcctgg tgaaggcccc gaaggccgcc tcctggccgt agtggcgcgc
     2581 cgagtacacc acgacttccg gcccctgggc ggcggccggc cccggggcgg cccacgtcgc
     2641 gaggatggtg gcgtccagag ccaggagggt tgcgagggtt cggatctcgt gtcccatcgg
     2701 tccgcgtctt cctttctctg ccgctcgccg gcccggcggg cgagcttggt cgtcgccgcg
     2761 cgcccacgct atactgacct cgagagcggc agaaattagg gttacctaat atggggaaga
     2821 cgtcaagcct aaaatcaggg gccccggccg cgaccacggc ctcggtccag gactacctgg
     2881 cggcgatcta cgacctcggg ggcagcggtc gccccgtgat cggtgcgcgt ctggccaagc
     2941 acatgggcat ctcggctccg gccgtcaccg agcaggtcca gcgcctgacg cggggtggct
     3001 acgtgaaggt cggtcgcggc aaggcgctgg ccctgacccc gaagggccgc gagatcgcgg
     3061 aggtgatggc gcgccgccac cggctgctcg agcggtggct gaccgacaaa ctggggctcg
     3121 actggaccga cgcccacgag gaggcccacc ggctggagca cgccatctcg ccgcgcgtcg
     3181 aggagcggct ggccgagatg ctgggcatgc cgtcgacgtg cccgcacggc aaccccatcc
     3241 cgggcatggc gtcgccccgg cgagtggaac cgttccccct ggcccaagcg cccgaaggcg
     3301 cgaacgtggt cgtggagcgc atcaccgagg aggccgaggc ggacaagaag cttctcgagc
     3361 acctctggcg caacgacgtg cgcccgggcc gccgcctgcg catcgtggag gtcgccccgt
     3421 gggccggtac gatcaccgcg gccagcgacg accgcaccat tgcgctcggg ctgccggccg
     3481 ccgcgaagat ctgggtctac gatccgcgcc gaacggagaa ggacggcgag cgagcgtgaa
     3541 ggctctcggc ggcgccttcg tcgtcgtggc cttcttctac gtgttctacg ccggcgtcac
     3601 cacgctctgg tcctacttgc aggtggcgag cgtggtggac agggcctggg aggaccaggg
     3661 ccgcaacggc gccgctgcgg tgcgcaccgc gatcgtcaag ggcgccagcg aggccggcgt
     3721 gccgctggag gtcaggttcg tccaggtcgg tgaagacgag cggatcatgg ccgtggccgt
     3781 ccgctggtcg tttccggcgg tcagcttcag gggcgacacc ttcgtcgaaa ttccgctctc
     3841 gctccagcgc tcgtacgaga agcggcccta gttcttgacc ggcttccgcg cctcggcgat
     3901 cagcgccttg acccgctcgt ggtcctgccc ggccgggacc agcttcagga acttctccca
     3961 cgaggcgatg gcgcccggcg cgtcgcgctt ggactcgtag aggacctgtc cgcggtagag
     4021 cagcgccggc gcgtaattcg gatcgatggc gagcgccttg tcgaacgtct gcagcgcgct
     4081 gtcggcgtgc ccgccgatgg ccacgatcag gccgagatgg gtcatcgcgt ccacgttgtt
     4141 cggatcgcgc ttgagcacgg cctggtacgc cgcgatcgcc tcgccatagc gacccgcgcc
     4201 gagcgagctc cgcgcagcct ccaacatgcc gcgcagcatc tcggggctca ccggccgcgg
     4261 cgccccgccg ccggccgggg cctcccccgg cgtctccgcc atcggcgcgc tgccacccgc
     4321 tggggacatg ccaccgggtc cggacatgcc accggcaccg ggcatgccgg gtggcccggg
     4381 catgccggcc gtggcgtcgg gctccgagta gcgcacgatg ccgacgccga gcaccacacc
     4441 gaagacgacg agcgcgacgg cggaggccgc gagggcgagc gggtggcgga agccgcgcgg
     4501 cgcgggcgcg gtggaggacg cgcgcgggac cgcagtcttg accacgggct ccggacccag
     4561 ccggtccagc tcggtcagga tctgcgccgt ctcggcctcg taacgggccc ggaggtcggc
     4621 gtagtcgtcg tcggacacgt gccccgcctc gtgctcgaag tcgagctcgc gcagggtgag
     4681 gagggcgtgg cgccgctgtt cgaggagcgg ctcgcgcggg tcgggcggta cggccaggag
     4741 cgcacgcgcg cggcgcccgg agagcagcgg ccagagcgtg aaggcggcag ccggaagtcc
     4801 gaggagcagc gcgacgaggg cgaccgtcat gcctcgcgct ccatctcccg gcggatgcgc
     4861 tcggccatcg cggggtcaac cggcgcctcg gcccggcggg ccgcccggcc gcgacgggtc
     4921 cagcggccca ggagcacggc gaccacggcg agacccacca cggcggcgac cggcggcacg
     4981 acccacacga gcagcgtgaa gccgctgcgc cggggctcga ggaggatcca ctcgccgtac
     5041 ttgtccacga agtactgcac cacctgctcc ggcctctggc ccgtggcgag gcgctcgcgg
     5101 atgacgccac gcatctggct ggccatctcc gacggcgagt cagcgaccga gaggttctgg
     5161 cacacgacgc agcgcagtcg ggccgcgatt tcgtgggcct cctgctcgtc gacgtggcgc
     5221 gcaggctgct gcgcgaggcc gggagtgacg gtcccgatga gcgagagcgc ggcgagcact
     5281 ccggtcgcga ggcggatcac ggatcgaatc atccgctcta gcctacacta ccggccggtg
     5341 ctcgataggc ccatcccggt cgtgaacctc catccgagtg accggcacgc ggcgtgcggg
     5401 acggcgctgc atgagtcggc gtgcccgtct cggtggggac agggctccgc gtgactgaac
     5461 cggcgagggt acggaagacg gagcggaccg acgtgtgtcc ggccggcggc ggggccgcgg
     5521 accgggcgcg gcagtgggtc gcccgggcgt tcacccgcct ggaagacgtc gtctacgttg
     5581 gtctgagcgt gttgctcgcg ggtaccgcgc tggggctgct cgccaccggg ctcatcgact
     5641 tcgtccggct cctgatcacc ggcaccctgt cgtcgtggcc ctgctcgacc gcgtgctcct
     5701 gatcctcgtc ttcgtcgagg tgctctacac ggtgcaggtt tcgttccgcg agcacgcgct
     5761 cgtgcccgag ccgttcctca tcgtcggcct catcgcggcg atccggcgtg tgctggtgct
     5821 gaccgccgag ctggccgagt tgacggagcg gggcgagggc gcctttcgca acgcgatcgt
     5881 ggagccgggg ctgctgacgg cgctcatcgt ggcgctcgtg ctgtcgctcg tgctcctgcg
     5941 ccggcggccg ccccacgccg tggccgaccg ggcctgacgc agccgtgagg gtctctcacg
     6001 ggggctcggc gagcagccgc tcgacggtgg tgcggaagac ggcctcggtc accgcgccga
     6061 cgtgcttgac gcggatcacc ccgcggcggt cgatgaagaa cgtctcgggc acgccgtaga
     6121 cgccgtagtc gatggagacc ttgccgcccg cgtccggcgc gttggggaac gtcaggcgga
     6181 agtcgtcgat gaacttgcgc gccttctcgg tcgtgtcctg aatgtccaca ccgaggacga
     6241 ggacgccgcg ctcgcgccac tcctgccagc cgcgctcgag caccggggcc tcctcgtagc
     6301 acgccgggta gcaccacgag gcccagaagt tcagcaccac gaccttgccc cgcagcgcct
     6361 cgagcgacac cggctcgccg gcgaaggtcg tcagcgcgaa gggggccgcc ggccgcccga
     6421 cgagcggcga cggcacgtcg cggggattgg tgcggaagcc gtaagccagg agcgccagca
     6481 cggggaccgc cgacagcgga atgagccaca ccagataccg cttcacgccg ccttccgccg
     6541 ctccggcatc accgcgagcg cggcgccgag cgtgaggacg agcccgccga tccagatcca
     6601 gctcacgagg cggttcacct ggatcttgac cgtcgcctgg ctgccatcgc gcgtgaagtc
     6661 gccgaggacg aggtacaggt cctcggtgag gccgagccag tagtccacgc cggcgagcgg
     6721 cgactgctcc tgagggtaga atttcttcga cggctgcatc acggccggcc gcgtgcggcc
     6781 gttctggacc gtgaagcgac cgacgacctt gaagtgattg gactcttcca cggtcccgag
     6841 gccgtcgaag cggacctggt acccggcgag ttgtgccgcc tcgccgacct tcaaggtcgt
     6901 ctccgtctgc accgaccacg cctgcgaccc tgtcacgccg acggcgatca ccaggatgcc
     6961 gaggtgcacg atgaacccgc cgtagcggcg gttgtggcgc gtgagcagtt cgcccagggc
     7021 gggcagcagc cgttcaccca tccgctgccg cgcccgggtg gcccggccga ggtcgagcgc
     7081 gatggtggcg gcgacgaaaa tcgtcagcgc gagcgagacc agcgcgagcg tcgagcgcac
     7141 gccgagagcg aagaagaccg cggcggcaac gaccccgacg gcgaccggac cgacgaagtt
     7201 ccggcgcagg ttgccgagcg aggcgcgccg ccaggcgatg agcggcccga cgcccatcag
     7261 gaagaccagg ccgaggaaga gcgggacgtt gacctggttg aagaacggcg cgccgacgct
     7321 caccttgacc ccgcggacgg cctcggacag cagggggaac acggtgccga agaagaccgt
     7381 gaaggtcgcc gccaccagca tcacgttgtt gagcaggaac gccgactcgc gcgacacgac
     7441 ggagtccagc tcgccctggg cgcggagggc gtcccaccgc caggccagca gccccagcgc
     7501 gagcagcagc acgaccgcca ggatgccgag gaagaagacg ccgacggagc cctgactgaa
     7561 ggcgtggacc gacccgatca cgcccgagcg ggtgaggaag gtgccgaaga gcgtcagccc
     7621 gaaggtgagg atcaccagcg tgacattcca gagcttcagc atccgccgcc gctcctggat
     7681 catcaccgag tgcaggaacg cggtacccgt gagccagggc atgaacgcgg cgttctccac
     7741 ggggtcccac gcccagtagc cgccccagcc gagcacgtga tagctccacc agccgccgat
     7801 gaggaggccg agcgacagga agtaccaggc ggtgatcgtc catcgccgcg tcagcgtgag
     7861 ccaggtgtcg ccgacccgac ccgtcgccag cgcggcgatg gcgaaggcga acggcaccgt
     7921 gaagccggtg aacccgaggt acagggccac cgggtgggtg atcatgccgg tgtcctcgag
     7981 caacgggttg aggccgcggc cgtcggccgg caccggcgtg agccgcgtga acggcggcgc
     8041 ggcgatggtc atcaccgaca ggaagaacgc catcaccccg agcatcacgg ccagcgccca
     8101 cgggtacagc tcgcgcgcgc gcacccgctg gcggacgatg aggaccagcg tgtagagcgc
     8161 gagcatccag ccccacagga tcaacgagcc ttccagcgcg ccccagacgg cggtgacgcg
     8221 gtaatagtac ggcgtgccga gattcgtgtt gccggccacg tagcggacgg agaaatcgaa
     8281 cgtgaggaac gcgtacacga gcagcccgag cgcgaaggtc accagggcga acacccccag
     8341 cgccgcgtgc tgcgcggatt ccacgagcgc cgggcgcgcc gtccccgcgg cggcggcgga
     8401 cgccaccatg ccccacaacg ccagcgcgaa cgcgacgacg agcgcaccct ggccgagctc
     8461 gggcgtcatg agccgggttt ccgcagggac ttcagcagct cgcggtaccc cgcgtcgctc
     8521 gcgtcgtgtg gggccttgta ctcctcggag tgcttggcca ggatgagcga ggccttgaag
     8581 tggccctcgg gcgtccactg gccctcgacc acggcgcctc gcccctcgcc gaagagatcg
     8641 ggcgcggtgc ccttgtggcg gacggggatg gaggccttgc cgtcggtcag cgtgaagctg
     8701 agatcgagcg tgcgcggctc ccagcgcagc gagcccggcg tcacgagccc gcccagccgg
     8761 tacgccttgt tcgtgaccgg ggcggcccgc agctccgagg gcgtgacgaa gtagaccgcc
     8821 gactgggtca cacccgcgta gatcatgtag acgatggccg ccgcgatcac gagcccgccc
     8881 gcgacgaact tggttttcat gctattgccc cgggggccgc gaggccggct cgccgcgtgg
     8941 gtgacccgac ccagcagcgg cgcgaatgcg ttcggcttcc cgatcgatcg agccggagcc
     9001 accgagccca gccgccgcgc cggccgtcgg ttccgtcgtc gtgcgatcct tggtccgcgc
     9061 ccggggggcg tcggcgcggg cggcgagcgc ggagagcgcg cgatcgagcc ggaccaggtg
     9121 gcgccagtag gcggccagca cgaaggcggc gaggccgtag gcggcgaaga cgtaccccca
     9181 gttgtccggc atcaggccag gacctcctgt tccctgcgca gcagccccac ccgcttggcc
     9241 acgaagtagg cggcgagcag cccgagggcg acgaagttga tcaggatggc gctccagaac
     9301 accgccggca tggtggcgtt ccccgccttc atcagcgacg ggggctggtg gagcgtgcgc
     9361 caccagtata ccgacgtgtg caccaggtag atgctcggcg cggcgaggac gccgaggacc
     9421 gcggcgaagc gggcgctgcg ctcggggtcg tcggtcatgc gacggaggag caggtagccg
     9481 aggtacatga ggaacacgac cgacacgctg gtcagccgcg cgtcccacgt ccaccacgtg
     9541 ccccacgtgg gcttgcccca gatcgagccc gtcgcgatgt tgatccccag gaacacgacg
     9601 ccgacctcgg tcgacgccgc ggcgagccgg tcggcgccgc gcgagcgcgt ccacagatac
     9661 ccgagtgacg tcccgaagac gaccaggaac gccagatagg cgacccacac ggccggcacg
     9721 tggacgtaca tgatccgctg cacgttgccc tgcgtggcct cgcgcggcgc cacgccgaag
     9781 gccgcgacga gaccggcggc gagaccgacg acggcgagcc agccgaacgt ccgggtcgcc
     9841 gtcgtcatgg ctcgaggatc gcgtcgaacg tgaggaggcc caccgtcacg tagatcacgt
     9901 cggcggcgac gaggagcttg agccagtgcg ccacggcgcc gagcggctcg ccggccagca
     9961 ccgcctcggt ggccttcacg gcgccgagca gcaccgggac ctggacgggc agcagcagca
    10021 ccgggaagag cagctctcgc gcgcgcacgt gcgccgtcat cgcggcgaag agcgtgccca
    10081 ccgccgcgaa ccccacggtg ccgagcgcca gcacgagggc cagggggccc gggtgggcgg
    10141 tcacgtccac gtcgaagaac acggcgaaca gcgcgacgag gatcgcctcg acgagcagca
    10201 tcaggagcac gttgcccgcc accttgccga ggtagatcgc ggacttgtcg cccggcgcga
    10261 gcagcaggcc ctcccagcag tcgttctcgc gttcggcgag gaaggcgcgg ccgagcccca
    10321 cgagaccgct cagcacgatg cccagccaga ggagccccgg cagcaccgcg agcagccgct
    10381 cgcggtcgcc gagcgcgaac tggaagagga acagcagcag cagcgagaag aagaacagcg
    10441 cgttcagcgt ctccttggac cgccgctcgc tcacgaggtc cttccagagc acgatccagg
    10501 cgcggcgcgc gtagctcacg cgtcctccac gtgcagcgcg tagagtttcc tgagatcttc
    10561 ggcggtcagc gcggccgacg gcgtgtcgag caccacgcgg ccgccggcga ggatggcgac
    10621 gcggtcggcg acgccgagcc cgcggccgaa gctgtgcgtg gtgaggagaa cggcgccccc
    10681 cgcggccttg aaggcggtga gccgggcctc cagccacttg ccggcggcct ggtcgagggc
    10741 ggcgaacggc tcgtccagca gcagcacgcg cggccgggtg agcacgaggc gcgccagcgc
    10801 cagccgccgc ttcatgccga gcgagaaggt gcgcgcccgc tcgtcggcca ggcgctcgag
    10861 gtccacggag gcgagcgcgg tgcgcaggac gtcggcgtcg gccgcctgcc cgccgagcgt
    10921 cgcccagaac ctcagattct cgagcgcggt gaggtcgtcg tagacccagg tgccgtggcc
    10981 gacgagggcg acgagggcgc gcacgcgctc cgcctcgcgg gtgcagtcgt ggccggcgac
    11041 ccgcgcgatc ccgcgcgtcg ggcgcgccag cgtggccagg attcgcagca gcgtcgtctt
    11101 gcccgcacca ttggggccga gcagcgcaac cgcctcgccg gggcgcacgt cgagggcgac
    11161 gtcgtccagc acgaagtggc cgccgaacgc cttgcgaaga cccaccacgt ggacgagagg
    11221 ctcagcggtc atccgtcaaa gcgccccatt ctcgcgcacg ccccggaggc ggtcaaatga
    11281 cgcctggcat gatgccgcgc cgggaagcga gacgaaaagc gccgggcttg acgtcggtgg
    11341 tgttcgagcg cgggctttgc cggcgccact tcctgtccgg gtgggtgcgg aggcctcctc
    11401 cgagctacac gcgcatcgag cggagccgcg cgatgcgctc ctccatcggc gggtgcgtgg
    11461 agaacaggtt catgagcgcg cgcccgctga gcgggttcac gatgaagagg tgcgccgtcg
    11521 ccgggttggc ggccatcggc accacctgct gcgccatgtg cagcttctcg agggccttgg
    11581 cgagtcccca cgtcttgccg gccagccgcg ccccggtcgc gtcggcctgg tactcgcgcg
    11641 agcgcgagac ggccatctgc accagcagcg cggcgatcgg tgccagtatc gccatgagga
    11701 tcgccgcgaa cggattggtg ccgccctcct cgttgtcgcc gcgatgcatg ccgaacatcg
    11761 ccgcccactg cgccatgtga gcgagatagg tgatggcgcc ggccatggtc gcggcgatgg
    11821 tggcgatcaa cacgtcgcgg ttcttcacgt gcgccagttc gtgggcgagc accccctcga
    11881 gctcctcctc gtcgagcagc cgcgtgatcc cctcggtcac ggcaaccgcc gcgtgctgtg
    11941 gattgcgccc cgtggcgaag gcgttgggcg tgtcggtcgg gatcacgtac acgcgaggca
    12001 tcgggatgcc ggcacggctg gtcagacggc gcaccatcgc gtagagcccg ggcgcctgcg
    12061 cctcgtcgac cggcttggcg ccgtacatgg ccagcacgat ccgatcggag aaccagtagg
    12121 agaagaagtt catggcgagc gccatcacga aggccatcac catgccttgc tggccgccga
    12181 tggcgccgcc gatcaacacg agcatggccg tgaggatcgc cagcagcatc gcggtcttga
    12241 aaacgttcga catgaagccc ctccttcgtg ggaccgagcc ggcgaaactt cgtagcgaag
    12301 cgtagcaaac gccccgggca cggtcaaatc gaccgtcaaa gatcttgact tgcccacccc
    12361 ccgccgatac actgggccgg cttcgggaaa agtcttctaa tgacaacgag ttacgcgggg
    12421 tccgccccgc gggaagcggt agccatgatc atccggctgt ccgagatcga ggacgacggc
    12481 ctcgtcgtcg ccaacgccgg cgagttcgtc gcgcccttca gcgatcgctc ctggcgcctg
    12541 gacggcctgc gcctacacgt cgcccgcgac ggcgaggacg tggtcgtcac cggccgcatc
    12601 gacgccaccg tcccggtggt ctgctcgcgg tgcctcgagg agttccgcgc ggatgtgcac
    12661 cccgtggtgg acgcacgcta cgtcccgaag ccgacggcgg gtcacgacgt cgagctcggg
    12721 gccgacgacc tcgacctgga tttctacgag aacgacgagt tgaacctggc ggcgctggtc
    12781 gagaccgaga cgacgctggc gctgccgatg aagcccctct gccgagccga ctgccggggg
    12841 ctctgccccg tctgcggcgt caaccggaac gtcacaccgt gcacgtgcga gcggcgcgcc
    12901 cccgacccgc gtctgtcggc gctgcgcgac ctggccgccc ggctcaacca ctgagaggac
    12961 ccgccgatgc ccctgccgaa gcgacgccac tcgagagcgc gcggccgcaa gcgccgcacg
    13021 cactacaagc tcggtgcgcc caccaactcc gtgtgtcccc agtgccggga gtccaagctc
    13081 ccgcaccggg tctgccccca ctgcgggtac tacaagggcc gggagatcat cgccgtcgag
    13141 ggcgcctgag accggagccg ccggccatcg ccacgatccg tagccacgga tggccatgaa
    13201 gattgccgtc gatgccatgg ggggggacta cggccccgcc gtggtggtgg agggggccgt
    13261 gtcggccgcg cgcgagcacc gagcgtcggt gatcctggtc ggcgacaagg cggccgtgga
    13321 gcgcgaggtg gtccggctcc aggccgagcc cctgggtctg gagatccgtc acgcctcgca
    13381 ggtcgtcggc atgggcgaga gcccctcgca ggcgctccgg cgcaagcgcg actcctcgct
    13441 ccgcgtggcg gcgcagctcg tcaaggacgg ggaggcggcc gccttcgtgt ccgccggtaa
    13501 caccggcgcg gcgatggcca ttgccatgtt cgtgatcggc gtgctgccgg gcgtcgaccg
    13561 gcccgcgatc gccgccgtgc tgccgaaccg ccgccggttc acggtcctcc tcgacgtcgg
    13621 cgccaacgtc gaccccaagg cctggcacct gctgcagttc gcggtgatgg gccacgtcta
    13681 cgcgcgcgac atcctgggtc tggacaaccc ccgggtcggc ctgctctcgg tgggcgagga
    13741 ggagggcaag ggcagccagc tcacgcggga agcctacgat cagctcaagg actcgtcgct
    13801 gaatttcatc ggcaacgtcg agggccgcga catctacaac ggccgctgcg acgtggtggt
    13861 caccgacggc ttcaccggca acgttgcgct caagatctcg gagagcctgg tcgagacgct
    13921 gggggagatg atcaaggaag agctcacgcg cgacgtgcgc tcgaagctgg gcgcagcgct
    13981 cgcgctgccg gcgttccatc gcttcaagcg ccgtgtcgac tacacggaga tgggcggcgc
    14041 gcccctcctc ggcatcgacg gcgccgcgat cgtctgccac ggcgcctcgc ccgtgaaggc
    14101 catcaagaat gccgtgcggg tggccggcga atgggccaag gcgggcgtga acgagcacat
    14161 cagggccgcg ctcgaggccg aagtgacacg cggggttcgg gagggaggcc gcgaatgacg
    14221 cgggcgaaga tcatcggcgt gggggcgtat gcgccgaagc gcatcctgac caacgccgac
    14281 ctcgagaaga tggtggacac caacgacgcg tggatcgtgc agcggaccgg catccgcgag
    14341 cgccacatcg ccgacgaggg cgaggccacc tccgacctcg cgctgcgggc ggcccagcag
    14401 gcgctagagc gcgcgaacct cgtgccggag gacatcgact tcatcgtggt cgggacgacc
    14461 acgcccgaca tgttcttccc caccaccggc aacatcctgc agcatcggct cggctgccgc
    14521 cgcgccggct ccgtggacct gctggccgcg tgcgcgggct cggtgtacag cctcgccgtc
    14581 ggcgcgaagt tcatcgaggc cggcaagtac cgccgcgtgc tctgcgtcgg cgccgagacc
    14641 ctgtctcgga tcacggactt caccgaccgc ggcacgtgca tcctgctcgc cgacgccgcc
    14701 ggcgccgtcg tgctggaggc gggcgaaggc gaggccggga tcatcgacgc cgacctctac
    14761 tccgacggcc agtactggga gctgctctac atgcccgggg gcggcgcgcg ccacccggcg
    14821 acgcgcgaga cggtcgacgc gcgcatgcac tacgcgaaga tgaagggcaa cgacgtcttc
    14881 aaggtcgccg tgcggatgtt cgtcgaatgc acggagacga tcctcgggcg ccacggcttc
    14941 accgcggccg acgtgaacct cttcatcccg caccaggcga acctccgcat catcgaggcc
    15001 gccgtcaagc gcgtgggcct gccgatggag aaggtgttca tcaacgtcga ccggtacggc
    15061 aacacgggcg ccgcgtcgat ctacgtggcg ctggaggaag ccgtcgcggc cggccgcgtg
    15121 aagcggggag acctcgtgct gctcgccgcc ttcggcgggg gcttcgcctg gggcgccgtg
    15181 ctgctccggt ggtgacgacg gacggcgacc gcgcgatgag ccgctcacgg gggtcgcggc
    15241 cgaacgcggg ccacgggcgc cggtgcccgg ttcgatgatc gcatttctct ttcccggcca
    15301 gggctcgcaa gcggtgggca tgggccgggc cttctacgag acctcgccgg cggccaagac
    15361 cgtcttcgag gaggcgaacg acgcgctcgg cttcgacctg gcgcgcctgg ccttcgaggg
    15421 gccggaggcg gcgctgacgc tcaccgcgaa cacccagccc gccgtcctca ccgtcagcat
    15481 cgccgccgct accgtggccg ccgagcgggg cctgcgtccc gcgatcgccg ccggtcatag
    15541 cctgggcgag tattcggcgc tcgtcgtggc gggggcgctc gcgttccgtg acgccgtgcg
    15601 cgtcgtgcgc cgccgcggtg agttcatgca ggacgcggtc ccggtgggca cgggcgcgat
    15661 ggccgccgtc atggggatgg acctcacggc cgtggaacag gtgtgcgtgg aggccggggc
    15721 gggcgaggtc gtcgaggtcg ccaacgtcaa ctctccgcag cagatcgtca tcgccggcca
    15781 caagacggcg gtcgagcgcg cggtgagtct ggccgccgcg cgcggcggcc gcaagagcgt
    15841 ggtgctgccg gtgagcgcgc ccttccactg cgccctgatg cggccggcgg cggagcgcct
    15901 ggcgcgtgag ctcgacggcg tgctcgtgac cgacccacgc atccccgtgg tgcgaaacgt
    15961 ggacgccggc gtgacccgcg tcgccgcgga ggtgaagccc gcgctgctcg agcaggtggc
    16021 gcgtacggtc cgctggaccg cgtgcgtgca gcgactcgcc gacgagggcg ccacgcgctt
    16081 cgtcgaggtc gggccggggc gggtgctcac cggcctgctc aggcgcatcg tggacggcgc
    16141 gcgcggggtc gcggtcgaga cgccagacga tctcgaccgg gccctcgagg acgcgacgtg
    16201 agcacggcgc cgctggcggg gcgggtcgcc atcgtgaccg gcggcagccg cggcatcggc
    16261 gccgccgtcg cggcgttgct ggtggagcac ggagccgccg tggtagtgtc gggccgcgac
    16321 cccgaccgcc tcgagcgcgc cgtgaaggag ctcgagggcg gcggcggcac cgccctcggc
    16381 gtcgtcgcgg atgccgccag ccgcgaggac gccgagcggc tcgccgacac cgcccggcag
    16441 cgcttcggtc ggatcgacgt gctcgtcaac aacgccggcg tcacgcgcga cagcctgctc
    16501 ctccgcatga aggacgagga ctgggatcgg gtgatggacg tgaacctgcg cggggccttc
    16561 gtgctgacac gcgcggtggc gaaggtgatg gtgcggcaga aggacggacg gatcatcaac
    16621 gtggcctcca ccgcaggcgc gatgggcaac gccgggcagg tcaactactc cgccgcgaag
    16681 gccgggctca tcgggttcac gaaggccgcc gcgcgggagc tcgcccactg gcggatcctc
    16741 gtcaacgccg tcgcccccgg gctcatcgag accgacatga ccgcgacggt gcccgaggcc
    16801 gcgcgccagg cgctgctcgc ccaggtgccg ctcggccgca tcggcacggc gcgcgaggtg
    16861 gccgaggtgg tacgattcct ggccggagac ggggcggcct acgtgacggg acaggtattc
    16921 cacgtcaacg gcgggctcta catgtagggc attccttcgg ggaggtgacg tgcgatggcg
    16981 aagtcagtgg aggacaaggt caaggagatc atcgtcgaac agctcggggt cgaggaggac
    17041 gacgtgaccc cgaccgcgaa gttcatcgag gatctcgggg cggactcgct cgacaccgtc
    17101 gagctcgtga tggcgctcga ggagcacttc gacatccaga tccccgacga ggacgccgag
    17161 aagatcgtga ccgtcggcga cgccatccag tacatcaagg acaactcctg acggtggatc
    17221 gccgccgcgt cgtcgtcacc ggcctcggcg cgctcacgcc gctgggcaac acggccgagg
    17281 agttctggac gggcctcgtc cagggccgct ccggcatcgg gcccatcacc aagttcgacg
    17341 cgggcgagaa gaccgcgagc ggcgagtacc ggtacgagac gcgcatcgcg ggcgaggtgc
    17401 gcaacttcga cccgctgaag ttcgtggaca agaaggacgc gcgccgcctc gacccgtatc
    17461 tccagtacgc catcgcctcc tcggcgatgg cggtggagga cgccggcctg gacgtcgacg
    17521 cgatcgatcg cgaccggttc ggcgtcctca tcggctccgg catcggcggc atctcgacgc
    17581 tgctcgaggg cgagcgcgtg cggctggaaa agggcccgga gcgcgtctct ccgttcatca
    17641 tcccgatgct catcgtcaac atggcgtcgg gcctcgtctc gatgcgcttc ggcgccaagg
    17701 ggccgaactc cgcggtcgtc accgcctgcg ccaccggcaa ccacgccatc ggtgacgcgt
    17761 tccggctgat ccagcgtggc gacgcggacc tgatgatcgc cggcggcgcc gaggccatca
    17821 tcgtccccct gaccatcgcg ggcttctgcg ccatgaaggc catgtccacg cgcaacgacg
    17881 cacccgagaa ggcctcgcgc cccttcgacg cggaccgtga cggcttcgtg cccagcgagg
    17941 gtgccggcat cgtgctcctc gaggccctcg agcacgcgcg gcggcgggac gcccgcatct
    18001 acggggagat cgtcggctac gggatgacgg cggacgccca ccacatgacg gcgcccgacc
    18061 cggagggcga cggcgccttc cgcgcgatgg cggcggcgct ccgcgacgcc gggctcgagg
    18121 caggccgcgt gggctacatc aacgcccacg gcacgtccac gccctacaac gacaagttcg
    18181 agacggtggc catcaagcgt ctcttcggcg accacacgcg ccggctcgcg gtgtcgtcca
    18241 cgaagtcgat gacgggtcac ctgctgggcg cggcgggggg cgtcgaggcc atcgccaccg
    18301 tgctggccct caaccacggg ctgttgccgc caaccgtgaa ctacgagacg cccgacccgg
    18361 agtgcgacct ggactacgtg ccgaaccagg cgcgcaagca ggacgtggag gtcgcgctca
    18421 gcaacgcctt cggcttcggc ggcaccaacg cgacgctcgt cttcacgaag ttccgcccgt
    18481 aagtgtcggg cgtcggcgac ctcgagcgcc ggctcggcca ccggttccgg gatggcgcac
    18541 tgctggagcg cgcgctgacc cacgcgtcct acgccaacga gcacccgccg cgggatcaca
    18601 acgaggcgct ggcgttcgtg ggcgacgccg cgctcgggct cgtcgtcgcc gaacggctct
    18661 tcgcggccga cccacaggcg cccgttggcg tgctgacgcc gcgccgcgcc gagatcgtgt
    18721 cgggcgcggc gctcgcgcga tgggcggcgg cgctggacct cggcgtcctg ctccggctcg
    18781 ggcgcggcga ggatcagacc ggtgggcgta cgcgcgagtc ggtcctggcc acgacgctgg
    18841 aggccgtgct cggcgcggta tacctcgagg ccggtctcga cggcgtgcgg cccgtcgtgg
    18901 cgcggctggc ggcggcatcg ccgccctcgg cgtgatagcc tgagcccatg cgtctctggc
    18961 cgtcgcagcg ccgccccgtc gtcgcggcgg tgccccccgc cgacctcgcc gtgaccgcgg
    19021 agctcgtacg gggcgccatc gaggacgtgt tcgccgcccg ccagcacggg gtcggctccg
    19081 cgggcgtcgt gttccggggc gagctccggc tgccgccagc gcgcgccgtc gacacgctgc
    19141 agcggcgcct ccgccccttc ggcttcacgc cgttcctgcg cgccgagggc ggcgacgtgg
    19201 tgctgcaggc cgtgcccgtc gtcgagcccg tggagcggcc ccgcgccagg gtgaacgtgc
    19261 tgctgttcgt gctcacctgt gcgagcacgc tcgccgccgg tgccttcttc tcgggctcgc
    19321 cgacgttcga cgccttccgg ggatcgctgg cggggacgtt cctcgtcgct ggcctcccgt
    19381 tcgcggcgac gctgctggcg atcctcggcg tgcacgagtt cggccactac ttcacggccc
    19441 gtcactaccg ggcgtcggtg agcctcccgt acttcattcc ggccccgccg ccgctgttcc
    19501 tcttcggcac gctcggcgcg atcatccgca tgcggtcgcc ggcgcccgac cggaactcgc
    19561 tcttcgacat cgccgcggc
//
LOCUS       DATIAZ010000230         2393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1507896, whole genome shotgun sequence.
ACCESSION   DATIAZ010000230 DATIAZ010000000
VERSION     DATIAZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507896"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VLF19_08195"
     CDS             complement(<1..183)
                     /locus_tag="VLF19_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93272.1"
                     /translation="MSAAEVRDRTGRLLLTAGEQITDRALRVLRMWGVEEIDVEGAVD
                     PPGPPVGHPTSAVFEGD"
     gene            complement(286..1008)
                     /locus_tag="VLF19_08200"
     CDS             complement(286..1008)
                     /locus_tag="VLF19_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSE93273.1"
                     /translation="MTVQWVVGAIGIAAASFVMGLAGFGIALVGLALLPFVMAPATAV
                     VLLTLYALVFAVVIAVQLRRDITPGPLATLLAGSVLGTPAGVWILASVSSAVLNRLIG
                     AVLIAVVVLEWRGLFPQRLAGRGWGLAAGVLAGFGGGAVGTPGPPVVVWCTAQGWAPR
                     TMKANLQAFFVVNQGVILTSYWWAGLLTPEVWRVAAAFALPALAGVAVGMALFRRIDP
                     VGFRRVVFGLLFLSGTALLLHG"
     gene            complement(1019..1897)
                     /locus_tag="VLF19_08205"
     CDS             complement(1019..1897)
                     /locus_tag="VLF19_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSE93274.1"
                     /translation="MTYVLVSGPPFQDIRYEIAERVATVTLHRPERLNAVTSRLLDEL
                     IAALDGADGDDEVRAVVVTGAGRAFCAGADLEAGGATFDSAVRDRAHAAEEHRDGGGR
                     VTLRIFDMKKPVIAAINGPAVGFGITLTLPMDVRLASTAARVGFVFTRRGVVPEACST
                     WFLPRLVGMSQAAEWMLTGRVFSAEEALAGRLVSRVLPPDELLPAARALAREIADHTS
                     AVSVALTRQMLWKLLGADHPIEAHRLDSQGMFWTGRSADAREGIGAFLDKRPARFTQR
                     ASADMPPFYPWWRRRE"
     gene            complement(1894..2175)
                     /locus_tag="VLF19_08210"
     CDS             complement(1894..2175)
                     /locus_tag="VLF19_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93275.1"
                     /translation="MRRALAAVAAVAALGGCTAALDLSGREWSKAGGDIRQITTDEIE
                     CVRLVSDIGETVESYVGGVADTVRFALRERSRVRGYDDCMTSRGYARTG"
BASE COUNT          372 a          887 c          850 g          284 t
ORIGIN      
        1 gtcgccttcg aagaccgcgg aagtcgggtg cccgacgggg ggaccgggcg gatcgacggc
       61 gccctcgacg tcgatctcct ccacgcccca catgcgcaag acgcgaagtg cccggtcggt
      121 gatctgctcc ccagccgtca gcagcagacg gcccgtacga tcgcgcactt cggcggcgct
      181 caccagcccc gcccgtgcct cctcgacccg gaccaccccc atcgcccgca atccacctcc
      241 gtctcagggg ttttcggcag cggggcgcgg gtcctgaacc cgcccttagc cgtgcaggag
      301 caaggcggtc ccggagagaa agagaagccc gaacacgacg cgccggaacc cgacggggtc
      361 gatgcggcgg aacagcgcca tcccgaccgc caccccggcg agcgccggca gtgcaaaggc
      421 cgctgccacg cgccacacct cgggtgtgag cagtccagcc caccagtagg aggtaagaat
      481 cacgccctgg ttgacgacga agaacgcctg gaggttggcc ttcatcgtcc gcggcgccca
      541 gccctgggcc gtgcaccaca cgaccacggg cggcccgggg gtgcccacag cgccgccgcc
      601 gaacccggcc aggacgcccg cggcgaggcc ccagccgcgg ccggccaggc gctgtggaaa
      661 cagcccgcgc cattcgagca cgacgacggc gatcaggacg gcgccgatga gccgattgag
      721 gacggcggac gacacgctgg ccaggatcca cacgccggcc ggcgtcccca acacgctgcc
      781 ggcgaggagc gtcgcgagcg gtcccggcgt gatgtcgcgc cgcagctgga cggcgatgac
      841 gaccgcgaag acgagcgcgt agagcgtgag cagcacgacg gcggtcgccg gcgccatgac
      901 gaacggcagg agcgcgagcc cgacgagcgc gatcccgaat ccggcgaggc ccatgacgaa
      961 gctggcggcg gcgataccga tggcgccgac gacccactgg acggtcacgc gggagcgatc
     1021 actcccgtcg tcgccaccac ggatagaagg gcggcatgtc ggcgctcgcc cgctgcgtga
     1081 accgggccgg gcgcttgtcg aggaaagcgc cgatgccctc gcgggcatcg gcggaccgcc
     1141 cggtccagaa catgccctgc gagtccagcc ggtgggcctc gatcgggtgg tcggcgccga
     1201 gcagcttcca cagcatctgt cgcgtgagcg ccacggacac ggccgaggtg tggtcggcga
     1261 tctcgcgcgc cagcgcacgg gcggcgggca gcagctcgtc gggcggcagc acgcggctca
     1321 cgagccgtcc cgccagggcc tcctccgcgg agaagacgcg gcccgtgagc atccattccg
     1381 ccgcctggct catcccgacg agccgcggca ggaaccaggt gctgcacgcc tcgggcacca
     1441 cgccccggcg cgtgaacacg aagccgacgc gggccgcggt cgaggccagg cgcacgtcca
     1501 tcggcagcgt cagcgtgatg ccgaagccga cggccgggcc gttgatggcg gcgatgacgg
     1561 gtttcttcat gtcgaagatc ctgagcgtca cccggccgcc gccgtcgcgg tgctcctccg
     1621 cggcgtgcgc ccggtcgcga acggcgctgt cgaacgtggc gccgccggcc tccaggtcgg
     1681 caccggcgca gaaggcgcgg ccggcaccgg tgacgacgac ggcgcgcacc tcgtcgtcgc
     1741 cgtcggcccc atcgagcgcg gcgatcagct cgtcgagcag ccgcgaggtg acggcgttca
     1801 gccgctcggg acggtgcagc gtgacggtgg cgacgcgctc ggcgatctcg taacggatgt
     1861 cctggaaggg tggacccgag acgagcacgt acgtcacccg gtcctggcgt agccgcggga
     1921 ggtcatgcag tcgtcgtagc cccgaacgcg gctccgctcg cggagggcga accgcaccgt
     1981 gtcggcgacg ccgcccacat acgactcgac ggtctcgccg atgtcgctca ccaggcgcac
     2041 gcactcgatc tcgtccgtcg tgatctggcg gatgtcgccg cccgccttgc tccactcgcg
     2101 tcccgagaga tccagcgccg ccgtgcaccc acccagggcc gccacggccg ccaccgccgc
     2161 gagcgcgcgt ctcatgtcgt gtcctccctc gccgccggcc ctcggccggg cgccgtcccg
     2221 cgtatgatac tgccatggac gtcgagatcc gtcggtgcct cgtctgagcc gacctccggc
     2281 gtgaggccgt gcgcctccgc gaggagatcc agcgaacgct ggggacgccc gcgaagattc
     2341 gctggggcgg gttcggggag ctgaccgtca cggtggacgg tcggaccgtc ttc
//
LOCUS       DATIAZ010000231         8962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_457440, whole genome shotgun sequence.
ACCESSION   DATIAZ010000231 DATIAZ010000000
VERSION     DATIAZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457440"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VLF19_08215"
     CDS             complement(<1..342)
                     /locus_tag="VLF19_08215"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSE93276.1"
                     /translation="MGFMDMRQWMGLLEKEGELRRITAEVDWDRELGAIARRVLEKRG
                     PALLFENIKGYRRGRCTKLLTGGLGDGRRLALALGFPKDAPNRELVQHVMRKNRETIA
                     PVVVRTGPVKEH"
     gene            complement(723..2588)
                     /locus_tag="VLF19_08220"
     CDS             complement(723..2588)
                     /locus_tag="VLF19_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HSE93277.1"
                     /translation="MRELRVALGAAWSLFQLYTAWAGSWDLLIQLPLHVAFATALGLL
                     TPAGDAPRPRWRSGLDVAAALLALACGAHYVVHNERLVTRMAMVDDPWTADVVVAVLF
                     VALLLEAARRHTGPALVVLALAFVAYAFAGSWLPGFLSHGGVRWLKLLDQQTLTTGGI
                     FGIPTLVSATYIYLFVAFGAVMARGGLLRFFTDIALAVAGSTRGGAGKVSVISSGLFG
                     TVNGSAIANAVTTGSFTIPLMTRAGYRPEFAAAVEATASMGGQLIPPVMGAAAFIMAE
                     TLGVPYATIALAAAIPGVLYFVAVGVMVHFEAARQGLPVMARADLPRLGAVLRRDAHL
                     LAGPALLLWFLMEGRSPLFAGFWALVVAVATSWLRTETRIGPWAALDVLVDAARNAMP
                     VAVACATVGIVVGVVSITGLGLKLATGIVGLAQGSLLATLVLTMIAALVLGTGLPTSA
                     TYIITSIMAAPALVQLGLPTLVAHMFVFYFGILADLTPPTAISTYATSSIAGADVWKT
                     QWRAMLLALSGFIIPFSFAYDPALLLIGAGPLAIGLRAAAATLGIVMLGAGLIGYLAG
                     TTRWWERALLLAGAVLLIFPGGVTDAVGAACFAIVLASQRAGRRERVETLEPAAR"
     gene            complement(2581..4254)
                     /locus_tag="VLF19_08225"
     CDS             complement(2581..4254)
                     /locus_tag="VLF19_08225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSE93278.1"
                     /translation="MDEIAADWVLANGRIHTLDPRRPAATALAVHGDRVAALGGRADV
                     RTWRDRRTRVVDLRGATVVPGLVDTHAHLDREGLKLVHPSLERCRSIADIQAVVRRLA
                     AGRPKGEWIVTMPVGAPPFYLDPTAGLRERRWPTRADLDAAAPDHPVYIRGIWGYWNK
                     PPVTSIASSRALALAGITRATEPPKGVEIVRDAAGEPTGVFVEHNLIQVLEFTLMKTA
                     PRFTPADRLRALRESQRRYAARGVTAVYEGHGVASEVLRVYRESHERGQLGLRCTLAL
                     SPTWQGADEARRAIPELVSWAGGRGLGDDRLRVAGICLHYGGNAGVARILHDAQPYTG
                     WAGFVESANSPEDYRAQAALAAGHGLRVNTLVTRCLPEVLDIWEAVAREAPIRDRRWV
                     AVHLNVATDRQLARLRALGAVATTNPISYLWRSAAEEALRASGAADMLLPHRSLARHR
                     IPFGLATDNKPADPWLAFAAVVARRDMATGEVVGDRERLTRPQALHALTAGGAWITCD
                     ERERGVLAPGRLADLAVLDRDPLTMPLEEVAALTCRLTMVGGRLVHGDA"
     gene            complement(4242..5216)
                     /locus_tag="VLF19_08230"
     CDS             complement(4242..5216)
                     /locus_tag="VLF19_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HSE93279.1"
                     /translation="MKTILALMLVLATAGVAWAQQPMTLAFSTLDTGSAWYVYGATMA
                     KLLREVLPAGSNVDVKPFAGGVGNAKLVARNETPLGLSFTVTNRWAFEGKEAYDTKLE
                     NIRALVGGLDRYYLVAVAAKKLNVSSLKDIKDRKIPVRIFTQPVGSLGEFGGRQLLRA
                     YGLGYADIKGWGGSTTHVGYNIIVDAFKDGRADLLLAVVTPKHPSVTEITTFADVKFL
                     PIDAEAVGALAPLGYTTATMPADTFKNQPEAVSTVGFPTVVITNKDLPEPVAYTVTKA
                     LLDNKDALVRGHAGLAGFNPQMAWRPENVGIPLHPGAERAYREKGWMK"
     gene            complement(5234..6004)
                     /locus_tag="VLF19_08235"
     CDS             complement(5234..6004)
                     /locus_tag="VLF19_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE93280.1"
                     /translation="MLTGIDHIVIAVADLAAASKSYGTLGFTVVPGGRHPVGTHNALI
                     AFADGAYVELIAFYEPNPEHKWWAPLQRGGGLVDFCMQTDDLLADTRAFRTAGVEIDD
                     PSPLSRVRPDGYQLRWVLSIPRGPHKGVAPFLIQDETPREERVPGQIEHANGVTGIRT
                     VTVAVDDLATVRGWYAAALGQPGVAAKRDDLGAAGSRFTIGPHTLELVTPSNATSPLR
                     EWLDRRGASPWAATFTTAGATRRSLDAGLTMGARLSLV"
     gene            complement(6009..7016)
                     /locus_tag="VLF19_08240"
     CDS             complement(6009..7016)
                     /locus_tag="VLF19_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE93281.1"
                     /translation="MPRSPFGLTLTNRAVVLGVVKARDLLDITVAAEESGAFDAVWVG
                     DSLLAKPRLEALSLLSALAGVTSRVRLGVGCLATFVHRHPVLFAHQWASLDVISNGRS
                     WLAVCLGGPDEQSKAQALEHAAMQVRSSERVGRLEEGVVILRKLFAETGVSFTGRYHS
                     FESVTIEPRPVQKPYPIWIASNPTGLTWKGGGSASEAAVERAYHRVARHADGWMTNKL
                     SPDEFRREWGRIAAMARAEGRDPSRIGTALYHNLNINEDRQAALEESKKFLDTYYTSS
                     FSAAFVEAWTVAGGPKQCVEQLRAYLDAGVGHVALRLTSWDQRGQLRRFLDEVAPAFA
                     R"
     gene            complement(7022..7789)
                     /locus_tag="VLF19_08245"
     CDS             complement(7022..7789)
                     /locus_tag="VLF19_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSE93282.1"
                     /translation="MSEFLRVERQGPVATLWIDRQPKMNTMTVAMRQAFPAIFAGLEA
                     DDGVRVIVVRGAGGKAFSAGGDVAEFLTLSPAELEQWGDTLTAAERCRKPVIAAIDGF
                     AMGAGLELAIACDFRIATRRSEFAFPEVRLGMIPGSGGTQRAMRLMGMTRAKLFMMTG
                     QRIAAERAEAWGLITQCVADDGLDAAVAALAGELAERPPLALRTLKMVLNRGADAALE
                     TALELERKAYAWLRSTHDYEEGVRSFLERRPPRYTGR"
     gene            complement(7879..>8962)
                     /locus_tag="VLF19_08250"
     CDS             complement(7879..>8962)
                     /locus_tag="VLF19_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93283.1"
                     /translation="PAPVATDLSLILYTSGTTGRPKGVPRTHRNHHAGAVAHALQCGY
                     AWGERTLGAMPLYHTMGIHALTSMVAVNGCFVCQPDWSVAGALRLIAGEHVTALYLIP
                     TLFYDLVHAPEAARTDLSSVRKLAYAGAPMLAPLTEACVKAFGPQIFVNHYGSTEIYT
                     FSVRPDVHRKPGCAGRPGLHSALRVVTASAERRVGPAEVVPRGEKGEIIARLDSEEAF
                     SGYWNRPDADARALRDGWYFTGDMGWVDADGELYVAGRVDDMIISGGENIHPVEVEEA
                     LARHPAVRDVAVVGERDERWGERVVAFVVPATADLTAEALDRFCRESPDLASFKRPRR
                     IVFVTEIPKTASGKVLRRLLRDAGTA"
BASE COUNT         1298 a         3196 c         3278 g         1190 t
ORIGIN      
        1 gtgctccttc acggggcccg tacgcacgac gaccggcgcg atcgtctccc ggttcttgcg
       61 catcacgtgc tggaccagct cccggttcgg cgcgtccttg ggaaaaccga gggccagcgc
      121 cagccgccgg ccatcaccga gcccgccggt caggagcttc gtgcaccggc cgcgcctgta
      181 gcccttgatg ttctcgaaga gcagcgccgg gccccgcttc tccaggacgc gccgggcgat
      241 cgcgcccagc tcgcggtccc agtccacctc ggcggtgatc cgccggagct cgccttcctt
      301 ctcgaggagc cccatccact gccgcatgtc catgaagccc atgtgtcgga tctcctacca
      361 acatgggggc cccgaaatgg ccctcaagcc cccagccacg ctgtggcgcg cctcgacact
      421 cccccgcgcc ccgaatgccc cccgagcccc cggcgctcgg cacgtcccag ccatgctgtg
      481 gcgcgctcga cacgccgcca ggttccggag ctgcgacgcg cctcgttcag tttccgacga
      541 cgtcgatcag gaccgccgcc agcaccgtga gcccggcgag cggaacgccc acaccggatg
      601 cccgagcagc ccgggccgct cgaagaacgg cggcctggac aggcgctgat gccgagcagg
      661 tcgtcgcccc accggacgat tgtagcggag ggcgacgcgt gccacggcga gatgggggcc
      721 cgtcacctgg cggccggttc aagggtctcg acgcgctcgc ggcgtccggc ccgctgcgaa
      781 gcgagcacga tggcgaagca cgccgcgccg acggcgtcgg tgacgccgcc cgggaagatc
      841 agcaggacgg cgccggcgag cagaagcgcg cgctcccacc accgggtcgt cccggccaga
      901 tagccgatga gccccgcccc gagcatcacg atcccgagcg tcgccgccgc ggcgcgcagg
      961 ccgatcgcca ggggcccggc gccgatcagg agcagcgcgg ggtcgtaggc gaacgagaac
     1021 gggatgatga agcccgagag cgccagcagc atggcccgcc actgcgtctt ccacacgtcg
     1081 gcgccggcga tggacgacgt cgcgtacgtc gagatcgccg tgggcggcgt gaggtcggcc
     1141 agaatgccga agtagaagac gaacatgtgg gcgacgagcg tgggcaggcc gagctgcacg
     1201 agcgcggggg cggccatgat cgacgtgatg atgtacgtcg ccgaggtcgg caggcccgtg
     1261 ccgagcacca gcgcggcgat catcgtgagg accagcgtcg cgagcaggct gccctgggcg
     1321 aggccgacga tgcccgtggc gagcttcaag ccgaggccgg tgatcgacac gacgccgacg
     1381 acgatgccga cggtcgcgca cgccaccgcg acgggcattg cgttgcgggc ggcgtccacg
     1441 aggacgtcga gcgcggccca cggaccgatg cgggtctcgg tccggagcca cgacgtcgcc
     1501 acggcgacca ccagcgccca gaagccggcg aagaggggcg agcggccctc catcaggaac
     1561 cacagcagca aggccggccc cgcgagcagg tgcgcgtcgc gccgaaggac ggcaccgaga
     1621 cgcggcaggt ccgcgcgggc catgaccggc agcccctggc gggcagcctc gaagtggacc
     1681 atgacgccga cggccacgaa gtagaggacg ccggggatcg ccgcggccag cgcgatggtc
     1741 gcgtacggca cgccgagggt ctcggccatg atgaaggccg ccgcgcccat gaccggcggg
     1801 atgagctggc ctcccatcga cgccgtcgcc tccacggcgg ccgcaaactc cggcctgtag
     1861 cccgcgcggg tcatgagcgg gatcgtgaag ctgccggtgg tcacggcgtt ggcgatggcg
     1921 ctgccgttga cggtgccgaa gagcccgctg gagatcaccg acaccttgcc ggccccaccc
     1981 cgcgtggagc ccgccaccgc gagcgcaatg tcggtgaaga agcgcaggag ccctccccgc
     2041 gccatcaccg cgccgaaggc gacgaagagg tagatgtacg tcgccgacac gagcgtggga
     2101 atgccgaaga tgccgccggt cgtcagggtc tgctggtcca ggagcttcag ccagcgcacg
     2161 cctccgtgcg agaggaagcc gggcagccag gatccggcga aggcgtaggc gacgaaggcg
     2221 agggcgagaa cgaccagcgc cgggccggtg tggcgccggg cggcctcgag gagcagggcg
     2281 acgaacagga ccgcgaccac gacgtcggcg gtccaggggt cgtccaccat cgccatgcgc
     2341 gtgacgaggc gttcgttgtg cacgacgtag tgcgcgccgc acgcgagcgc caggagcgcg
     2401 gcggcgacgt cgaggccgct gcgccagcgc ggccgtggcg cgtcgccggc gggcgtcagg
     2461 aggccgagcg ccgtggcgaa cgcgacgtgg agcggcaact ggatcagcag gtcccacgag
     2521 cccgcccacg ccgtgtagag ctggaacagc gaccaagcgg cgccgagcgc gacccgcagc
     2581 tcacgcatcg ccgtgcacca gccgtccgcc gaccatggtg agccggcacg tcagcgcggc
     2641 gacctcctcg agcggcatgg tgagcgggtc ccggtcgagc acggccaggt cggccaagcg
     2701 tccgggcgcc agcactcccc gctcgcgctc gtcgcacgtg atccacgcgc cgccggcggt
     2761 cagcgcgtgg agcgcctgcg gtcgcgtgag ccgctcgcgg tcaccgacga cctcgcccgt
     2821 ggccatgtcg cggcgggcga cgacggcggc gaacgcgagc cagggatcgg ccggcttgtt
     2881 gtcggtggcg aggccgaagg ggatgcggtg gcgcgcgagg ctccggtggg gcaggagcat
     2941 gtcggcggca ccgctggcgc ggagcgcctc ctcggccgcc gaccgccaga gatagctgat
     3001 cgggttcgtc gtcgccaccg cgccgagcgc ccgcaggcgc gcgagctgcc ggtcggtggc
     3061 aacgttgagg tgcacggcga cccagcgccg gtcgcggatc ggcgcctcgc gcgccaccgc
     3121 ctcccagatg tcgaggacct cgggcaggca gcgggtcacg agcgtgttca cgcgcagccc
     3181 gtggccggcc gcgagggcgg cctgcgcgcg gtagtcctcc ggcgagttcg cgctctcgac
     3241 gaacccggcc cagcccgtgt agggctgcgc atcgtggagg atgcgcgcga cgccggcgtt
     3301 gccgccgtag tgcaggcaga tgccggcgac ccgcagccgg tcgtcgccga ggccgcgtcc
     3361 gcccgcccac gagaccagct cggggatcgc acgccgggcc tcgtccgcac cctgccaggt
     3421 cgggctcagc gccagcgtgc agcgcaggcc gagttggccg cgctcgtggc tttctcggta
     3481 gacccgcagc acctcggacg ccacgccgtg gccctcgtag accgccgtga cgccgcgggc
     3541 cgcatagcgg cgctgcgact cgcggagcgc ccgcagacgg tcggcagggg tgaaccgcgg
     3601 cgcggtcttc atcagcgtga actcgaggac ctggatgagg ttgtgctcca cgaacacgcc
     3661 cgtaggctcg ccggcggcat ccctgacgat ctcgaccccc ttcggcggct cggtggcgcg
     3721 tgtgatgccg gcgagggcca gggcacggct gctggcgatg gacgtgaccg gcggcttgtt
     3781 ccagtagccc cagatgccgc ggatgtagac gggatggtcg ggcgccgccg cgtcgaggtc
     3841 ggcgcgggtg ggccagcgac gctcgcgaag gccggccgtc ggatcgagat agaagggagg
     3901 cgcgccgacc ggcatggtga cgatccactc gcccttcggg cgccccgccg ccaggcgacg
     3961 gacgaccgcc tggatgtcgg cgatcgagcg gcagcgctcc agtgacggat ggacgagctt
     4021 caggccctcg cgatccaggt gcgcgtgcgt gtcgaccagg cccggcacga ccgtggcgcc
     4081 gcgcaggtcg accacgcggg tgcggcggtc gcgccaggtg cggacgtcgg cgcgaccgcc
     4141 cagggcggcc acgcggtccc cgtgtacggc gagcgccgtc gccgccggcc ggcgcgggtc
     4201 gagggtgtgg atgcggccgt tggcgaggac ccagtcggcc gctatttcat ccaccccttc
     4261 tcgcgatacg cgcgctcggc gcccgggtgc agcgggatgc cgacgttttc gggccgccac
     4321 gccatctgag gattgaagcc cgccagcccc gcatggccgc gcaccagcgc gtccttgttg
     4381 tcgagcagcg ccttggtgac cgtgtaggcg acgggctccg gcaggtcctt gttggtgatg
     4441 acgaccgtgg ggaagccgac ggtgctgaca gcctccggct ggttcttgaa cgtgtccgcc
     4501 ggcatcgtgg cggtggtgta gcccagcggc gcgagcgcgc cgacggcctc cgcatcgatc
     4561 ggcaggaact tcacgtcggc gaaggtcgtg atctcggtga ccgacgggtg cttgggcgtc
     4621 accacggcca gcagcaggtc cgcgcgaccg tccttgaagg cgtccacgat gatgttgtag
     4681 cccacgtggg tcgtggagcc gccccacccc ttgatgtcgg cgtacccgag gccgtaggcg
     4741 cgcagcagct ggcgcccgcc gaactcgccc agcgagccca ccggctgggt gaagatcctc
     4801 acgggaatct tccggtcctt gatgtccttc agcgagctga cgttcagctt cttggccgcg
     4861 accgcgacga ggtagtagcg gtcgaggccg ccgacgagcg cccggatgtt ctcgagcttc
     4921 gtgtcgtagg cttccttgcc ctcgaacgcc caccggttgg tcaccgtgaa cgacagtcca
     4981 agcggcgtct cgttcctggc gacgagcttc gcgttgccga cgccgcccgc gaatggcttg
     5041 acgtccacgt tggagccggc tggcagcacc tcgcgcagca gcttggccat cgtggcaccg
     5101 tagacgtacc aggcgctgcc ggtgtcaagg gtggagaacg ccagcgtcat cggctgctgc
     5161 gcccaggcga cgccggcggt cgcaagcacg agcatcaggg cgaggatcgt cttcatcggt
     5221 cggatctcct ctctcacacg agcgatagcc gcgcgcccat cgtcagaccg gcgtcgagcg
     5281 agcgccgggt cgcgccggcc gtcgtgaagg tggccgccca gggcgaggcg ccgcggcggt
     5341 cgagccattc gcgcagcggg ctcgtggcat tggacggcgt tacgagctcc aaggtgtgcg
     5401 ggccgatggt gaaccggctt cccgcggcac ccaggtcgtc gcgcttcgcg gcgacgcccg
     5461 gctggccgag cgcggcggcg taccagccgc ggacggtcgc gaggtcgtcc accgcgacgg
     5521 tgacggtgcg gatgccggtg acaccattgg cgtgctcgat ctgacccggc acgcgctcct
     5581 cgcgcggggt ctcgtcctgg atcaggaacg gcgccacgcc cttgtgaggt ccgcggggga
     5641 tcgagagcac ccaccggagc tggtaaccgt cggggcgcac gcgcgagagc ggcgatgggt
     5701 cgtcgatctc gacgccggcg gtgcggaagg cccgggtgtc ggcgaggagg tcgtcggtct
     5761 gcatgcagaa gtccacgagt cccccgccac gctgcagcgg cgcccaccac ttgtgctccg
     5821 ggttgggctc gtagaacgcg atcagctcga cgtaggcccc gtcggcgaaa gcgatcagcg
     5881 cgttgtgggt acccaccggg tggcgcccgc ccggcaccac ggtgaagccg agggtcccgt
     5941 agctcttcga cgccgcggcg aggtcggcga cggcgatgac gatgtggtcg atgcccgtca
     6001 gcatggcgtc acctcgcgaa ggccggcgcc acttcgtcca ggaagcgtcg gagctgaccg
     6061 cgctggtccc acgacgtcag ccgcagggcc acgtggccca cgccggcgtc gagataggcg
     6121 cggagctgct cgacgcactg cttggggccg ccggcgaccg tccacgcctc cacgaaggcg
     6181 gcgctgaagc tggacgtgta gtaggtgtcg aggaacttct tcgactcctc gagcgcggcc
     6241 tgccggtcct cgttgatgtt gaggttgtga tagagcgcgg tgccgatgcg gctcgggtcc
     6301 cgcccctcgg cgcgcgccat cgcggcgatg cgtccccact cgcggcggaa ctcgtcgggc
     6361 gagagcttgt tcgtcatcca gccgtcggcg tgtcgcgcca cacggtggta ggcccgctcg
     6421 acggcggcct cgctcgcgct gccgccgccc ttccacgtga gtccggtggg gttgctggcg
     6481 atccagatcg gatagggctt ctgcaccggc cggggctcga tcgtcacgga ctcgaacgag
     6541 tggtagcgtc cggtgaagga cacgccggtc tcggcgaaga gcttgcggag gatgaccacg
     6601 ccttcctcga gacgccccac gcgctcgctc gagcgcacct gcatggcggc gtgctcgagc
     6661 gcctgcgcct tcgactgctc atccgggccg ccgaggcaca cggcgagcca cgaccgtccg
     6721 ttcgagatca cgtcgagact cgcccactgg tgggcgaaga gcaccggatg gcggtgcacg
     6781 aatgtcgcca gacagccgac gcccaggcgg acccggctcg tcaccccggc cagggccgag
     6841 agcagcgaga gggcctcgag gcggggcttg gcgagcaggc tgtcgccgac ccagacggcg
     6901 tcgaaggcgc cggactcctc cgcggccacg gtgatgtcga gcaggtcgcg cgccttcacg
     6961 acgcccagca cgaccgcgcg gttggtgagg gtgaggccga agggtgagcg tggcacggtg
     7021 gtcacctccc ggtgtagcgc ggcggccgcc tctcgaggaa cgagcggacg ccttcctcgt
     7081 agtcgtgagt ggagcgcagc caggcgtagg ccttccgctc gagctcgagc gcggtctcga
     7141 gcgcggcgtc ggcgccgcgg ttgagcacca tcttgagcgt ccggagcgcc agcggcggac
     7201 gctcggcgag ctcgccggcc agcgcggcca ccgccgcgtc gagcccgtcg tcggccacgc
     7261 actgtgtgat caggccccac gcctccgcgc gctcggcggc gatgcgctgg cccgtcatca
     7321 tgaagagctt cgcgcgcgtc atgcccatca gccgcatcgc ccgctgggtc ccgccgctgc
     7381 cggggatcat gcccaggcgc acctcgggga aggcgaactc cgaccgcctc gtggcgatcc
     7441 ggaagtcaca ggcgatcgcc agctccaggc ccgcgcccat cgcgaagccg tcgatggccg
     7501 cgatcacggg cttgcggcag cgctcggccg cggtcagcgt gtcgccccac tgctcgagct
     7561 cggcgggcga gagcgtgagg aactcggcca cgtcgccgcc ggcactgaac gccttgccgc
     7621 cggccccgcg gacgacgatc acccgcacgc cgtcgtcggc ctcgaggccg gcgaagatcg
     7681 ccgggaacgc ctggcgcatc gccaccgtca tcgtgttcat cttcggctgg cggtcgatcc
     7741 agagcgtcgc caccggcccc tgccgttcca ccctcaggaa ctcgctcatt tccctgacat
     7801 ggcgtcccga catggccccc aagccccctc gttcggcacc ccggcggagc cggggcgcgc
     7861 cttgcactcc gctggtcctc aggccgtccc ggcgtcgcgc agcaggcggc gcaggacctt
     7921 gcccgacgcc gtcttcggga tctccgtgac gaacacgatg cgccgcggcc gcttgaagct
     7981 cgccaggtcg gggctctcgc ggcagaaccg gtccagggcc tcggcggtga ggtccgccgt
     8041 ggcgggcacg acgaaggcga cgacccgctc gccccaccgc tcgtcccgtt cgccgaccac
     8101 cgccacgtcg cgcactgccg ggtgccgggc cagcgcctcc tcgacctcca ccgggtggat
     8161 gttctcgccg ccactgatga tcatgtcgtc gacgcgcccg gccacgtaca gctcgccgtc
     8221 ggcgtcgacc cagcccatgt ccccggtgaa gtaccagccg tcgcgcagcg cccgcgcgtc
     8281 ggcgtcgggg cggttccagt agccggaaaa ggcctcctcg gagtccaggc gcgcgatgat
     8341 ctcgcccttc tcgccccgcg gcacgacctc ggcgggcccg acccgccgct ccgcgctcgc
     8401 cgtcaccacc ctgagcgccg agtgcaggcc cggccgcccg gcacagccgg gcttgcgatg
     8461 gacgtcgggg cgcacggaga acgtgtagat ctccgtcgag ccatagtggt tcacgaatat
     8521 ctgtggtccg aaggccttca cgcacgcctc ggtgaggggc gccagcatcg gcgcgcccgc
     8581 gtacgcgagc ttgcgcaccg acgacaggtc ggtgcgcgcg gcctccggcg cgtggacgag
     8641 atcgtagaac agcgtcggga tcaggtagag cgccgtcacg tgctccccgg cgatcaggcg
     8701 cagcgcgccg gccacggacc agtcgggctg gcacacgaag cagccgttga ccgccaccat
     8761 gctcgtcagc gcgtggatac ccatggtgtg gtagaggggc atcgcgccga gcgtgcgctc
     8821 gccccacgcg tagccgcact gcagagcgtg ggcgacggcg ccggcatggt ggttgcggtg
     8881 cgtgcgcggg acgcctttcg ggcggcccgt cgtgccggac gtgtagagga tcagcgagag
     8941 gtcggtggcg acgggggcgg gg
//
LOCUS       DATIAZ010000232         4466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1641413, whole genome shotgun sequence.
ACCESSION   DATIAZ010000232 DATIAZ010000000
VERSION     DATIAZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641413"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..1169
                     /locus_tag="VLF19_08255"
     CDS             405..1169
                     /locus_tag="VLF19_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93284.1"
                     /translation="MAGRLEGKTAFITGAGMGIGREAARLFAAEGARVVVADIDGDAA
                     KETVLQVEAGGGRALAVTGDVAVEADVQHLIEHGVRHFGALHVLYNNAGVLWKDRDRS
                     VLETDERWWDRVMAINLKSVFWVTKHGIPHLKAAGGGSIIVMGSVSALAGFTRAQDAY
                     TAAKGGLISLTKSLAIQFAKDRIRCNIIHPGIVETPLQAPYLTDALRKEFETGIPLGR
                     VAQPREIAAVALFLASDDSSYMTGAELVVDGGFTAG"
     gene            1296..2894
                     /locus_tag="VLF19_08260"
     CDS             1296..2894
                     /locus_tag="VLF19_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSE93285.1"
                     /translation="MVLLLFVFVVWPVVRVLWMSVESPAGPTLAHYRDFFSTWRLFRI
                     LVNSLAVALVSTAITVVLALVLAYAVTRTTVPGKRLLGLMSLLPLISPPFLVSLALIL
                     LFGRNGVVTRALGLDWSIYGFHGIVLAQVFTFLPQAYILLANVLGNVDTTLEEAAENL
                     GAGPFTTLRRVTLSLARPGLASASLVVFILCMTDFGNPILVGGRVNVLATEIYAQIIG
                     MNDFAAAATMSVVLVVPCLAAYLLNAYWVGRRAYVTVSAGARTAARPTPALVRWPLFA
                     VSGGIALFIAVIYGLIPLGSVVRLWGSDWSLSLAHYGFRSTAEGAGPIWNSVKLAAVA
                     GVAGTIFALLAAYLVERKRASLGGPVARAIESLSLLPAALPGTVMGVGYILAFNVPPV
                     YLTGTIWILVASVVFWKLPVAVLAGVNALKQIDPAIEEAAVSLGAGSVRTFTRVVLPL
                     LTGTAFSIFAYFFINGMVTVSAVIFLIYPGFNLGSVAILAQVENGYPGVACALGTIIL
                     AIVVGTVLLLRALVGGDRVAILNA"
     gene            2914..3912
                     /locus_tag="VLF19_08265"
     CDS             2914..3912
                     /locus_tag="VLF19_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93286.1"
                     /translation="MRRILVLLAAALLLGGVGRAAAAEEKLVIYTAYEENELKTFWEQ
                     FKKDLPDLAARASYIRGSTGPTMARLEAERANPQADVVWGVFNDYLTGAAKKGLLERY
                     AAKESDRIAARFKHPDNLWQGVTLLTVAFATNQKRMAELKLSPPRTWSDLLDPKYKGH
                     IVMSNPSTSGTAYLLLASHVKRLGEDKAFEYYAALDKQLAQVTKSGGAPGRMAASGET
                     PIGIALAYEVEVARKQGARIDVIHPTDGVAWTFEGNALVKGAKHPQNARRFLDWAVSK
                     SAMTSYAEWRGTAITRTDVGVKGPNISELKLIDLDFVWAADQKERLVKKWLERFSR"
     gene            3914..>4466
                     /locus_tag="VLF19_08270"
     CDS             3914..>4466
                     /locus_tag="VLF19_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93287.1"
                     /translation="MRIALRGLTKRFGPTPAVDGLSLEIRPGELVALVGGSGCGKTTT
                     LRMVAGFERPDAGDIFFDDRRVNDVPPRRRGVGIVFQSYALFPTLTVAENIAFGLRVA
                     RWSEARVRQRVAEMVALTRLGGFERRYANQLSGGQQQRVALARALARQPAILLLDEPL
                     SALDAKIRLRLRGEIRKIQQDLGI"
BASE COUNT          626 a         1551 c         1580 g          709 t
ORIGIN      
        1 ctatctccat accgcgcgac tcgagcagca gtcattcgac caggccgtga cggactggga
       61 gctcatgcgc aacttcgagc ggatctagtc tagtgtgccg gaatcttgag gcggcggtcg
      121 acgccaccga ccggggcggg cccgccttcc cgggcggcgt ggcgggtccg ccgacctggc
      181 cccccacagc caggcgcccg gcaagggcgc agatggaccc acgcaacggc aggcgtcgcg
      241 ctgcgcatca ggggcgccgg acccgtcacg ccgcccgggc gggccggcct gccccggacg
      301 acggtcggcg agcttccgag tcgcgagacc ggcgaggtcg acgagaacgg ctcaccgatc
      361 tcgtcggcgt gtctgccgtt gtgaacgacc ccggggggca ctgaatggcc ggacggctcg
      421 agggcaagac ggcgttcatc acgggagcgg gaatgggcat cggccgcgag gcggcgcgtc
      481 tcttcgccgc cgagggcgct cgcgtggtcg tcgcggacat cgatggcgac gcggcgaagg
      541 agacggtgct ccaggtcgag gccggcgggg gccgcgcgct cgccgtcacc ggcgacgtcg
      601 ccgtcgaggc ggacgtccag cacctgatcg agcacggcgt ccgccatttc ggcgcgctgc
      661 acgtcctcta caacaacgcc ggcgtgctgt ggaaggaccg cgaccggtcg gtgctggaga
      721 cggacgagcg ctggtgggac cgcgtgatgg ccatcaacct caaatcggtt ttctgggtga
      781 ccaagcacgg gatccctcat ctcaaggccg ccggcggcgg gtcgatcatc gtcatgggtt
      841 cggtgtccgc gcttgcgggc ttcacccggg cccaggacgc gtacacggcg gccaagggcg
      901 gcctcatctc gctgacgaag tcgctggcca tccagttcgc gaaggaccgc atccgctgca
      961 acatcatcca ccccggtatc gtggagacgc cgctgcaggc gccatacctg accgacgcgc
     1021 tccggaagga gttcgagacg ggcattccgc tcggccgcgt cgcgcagccg cgcgagatcg
     1081 cggccgtcgc cctgttcctg gcaagcgacg actcgtccta catgaccggc gccgagctgg
     1141 tcgtggatgg cggcttcacg gccggctagg agggggccac gagcgctctg agcggaccgg
     1201 gcgcgggccg ggccggcgtc gcgggccccg ttccgctggc cccggccggc cggctggcgc
     1261 aggcggaggc ggccacgatc gcggtcgccg ccggcgtggt gctcctgctg ttcgtcttcg
     1321 tggtgtggcc cgtcgtgcgg gtgctgtgga tgagcgtgga gtcgcccgcc ggcccgacgc
     1381 tcgcccacta ccgcgatttc ttcagcacct ggcggctctt ccgcatcctc gtcaacagcc
     1441 tcgccgtggc cctcgtgtcc accgcgatca ccgtggtgct ggcgctcgtg ctggcctacg
     1501 ccgtgacgcg tacgacggtg cccggcaagc ggttgctcgg cctgatgagc ctgttgccgc
     1561 tgatctcgcc accgttcctc gtctcgctcg cgctcatcct gctcttcggc cgcaacggcg
     1621 tggtgacgcg ggcgctcggt ctggactggt cgatctacgg cttccacgga atcgtgctgg
     1681 cccaggtgtt cacgttcctg ccgcaggcgt acatcctgct cgccaacgtg ctcggcaacg
     1741 tcgacaccac gctcgaggag gccgccgaga acctcggcgc cggtccgttc accacgctgc
     1801 gccgggtgac gctctcgcta gcccggccgg gcctggcctc ggcatcgctc gtcgtgttca
     1861 ttctctgcat gacggacttc ggcaacccga tcctcgtcgg cggccgcgtg aacgtgctgg
     1921 cgacggagat ctacgcgcag atcatcggca tgaatgactt cgcggcggcg gcgacgatga
     1981 gcgtggtgct cgtggtgccg tgcctcgctg cctacctgct caacgcctac tgggtcggca
     2041 ggcgcgcgta cgtgaccgtc tcggcggggg cgcggacggc ggcgcgcccg acgcccgcgc
     2101 tcgtccgctg gccgctcttc gcggtctccg gcggcatcgc gctcttcatc gcggtgatct
     2161 acggcctcat cccgctcgga tcggtcgtcc gcctgtgggg gagcgactgg tcgctctcgc
     2221 tcgcgcacta cggcttccgc tcgaccgcgg agggcgccgg gcccatctgg aacagcgtca
     2281 agctcgccgc ggtggccggc gtcgcgggca cgatcttcgc cctgctcgcg gcctacctcg
     2341 tcgagcgcaa gcgggcgagc ctcggcgggc cggtcgcccg cgccatcgag tcgctgagcc
     2401 tgctaccggc cgcgctgccc gggacggtca tgggcgtcgg ctacatcctg gccttcaatg
     2461 tgccgccggt gtacctcacg ggcacgatct ggatcctcgt cgccagcgtg gtgttctgga
     2521 agctgccggt ggccgtgctg gcgggcgtca acgccctcaa gcagatcgac ccggccatcg
     2581 aggaggccgc ggtcagcctc ggcgccggca gcgtgcggac gttcacccgg gtggtcctcc
     2641 cgctcctcac gggcaccgcc ttctcgatct tcgcgtactt cttcatcaac gggatggtca
     2701 ccgtgagcgc ggtgatcttc ctcatctatc ccgggttcaa cctcggctcg gtggcgatcc
     2761 tcgcccaggt cgagaatggt tacccgggcg tggcgtgcgc gctcggcacg atcatcctcg
     2821 ccatcgtcgt cggcaccgtg ctgctgctac gagccctggt cggcggcgac cgggtggcga
     2881 tcctcaacgc gtagcgaccg aaaggagacg ctcatgcgac gtatcctggt gctgctggcg
     2941 gcggcactgc tcctcggcgg ggtgggccga gcggcggcag ccgaggaaaa gctcgtcatc
     3001 tacacggctt acgaggaaaa cgagctgaag acgttctggg agcagttcaa gaaggacctg
     3061 cccgatctcg ccgccagggc gtcctacatc cgcggctcga cgggacccac gatggccagg
     3121 ctcgaggccg agagggccaa cccgcaggcc gacgtcgtct ggggcgtctt caacgattac
     3181 ctcaccggcg ccgcgaagaa ggggttgctc gagcgttacg ccgccaagga gtccgaccgc
     3241 atcgccgccc gcttcaagca tccggacaac ctgtggcaag gggtgacgct gctcacggtg
     3301 gccttcgcca ccaaccagaa gcggatggcc gagctgaagc tctcgccgcc gcggacgtgg
     3361 agtgacctgc tcgatcccaa gtacaagggc cacatcgtca tgtcgaaccc ctccacgtcc
     3421 ggcacggcgt acctgctgct ggccagccac gtgaagcgct tgggcgagga caaggccttc
     3481 gagtactacg ccgcgctcga caagcagctc gcgcaggtaa cgaagtcggg tggagcgccg
     3541 ggccgcatgg ccgcgtccgg cgagacgccg atcgggatcg cgctcgccta cgaggtcgag
     3601 gtggcgcgca agcagggggc gcggatcgac gtcatccacc ccacggacgg cgtggcgtgg
     3661 accttcgagg gcaacgcgct cgtcaagggc gcgaagcacc cccagaacgc caggcggttc
     3721 ctcgactggg cagtctcgaa gtcggcgatg acttcctacg ccgagtggcg ggggacggcg
     3781 atcacgcgca ccgacgtggg cgtgaagggg ccgaacatct ccgagctcaa gctgatcgac
     3841 ctcgatttcg tgtgggcggc cgaccagaag gagcgtctgg tcaagaagtg gctcgagcgg
     3901 ttctcgcggt agcgtgcgca tcgcgctccg cggcctgacg aagcgtttcg gcccgactcc
     3961 cgcggtggac gggctgtcgc tggagatccg tcccggggag ctggtcgcgc tcgtcggcgg
     4021 gtcggggtgc ggcaagacga cgacgctccg gatggtcgcg gggttcgagc gccccgacgc
     4081 cggcgacatc ttcttcgacg accggcgggt gaacgacgtc ccgccgcgcc ggcgcggcgt
     4141 cggcatcgtg ttccagtcct acgcgctgtt tccgaccctc acggtggcgg agaacatcgc
     4201 gttcggcctc cgggtggcac gctggtcgga ggcgcgcgtt cgccagcgcg tggcggagat
     4261 ggtggcactg acccggctcg gcggcttcga gcggcgctac gccaaccagc tctccggcgg
     4321 gcagcagcag cgcgtggcgc tggcccgggc actggcgcgc cagccggcga tcctgctcct
     4381 ggacgagccg ctctccgccc ttgacgcgaa gatccgcctg cggctgcggg gcgagatccg
     4441 gaagatccag caggacctcg gcatca
//
LOCUS       DATIAZ010000233         9359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_63244, whole genome shotgun sequence.
ACCESSION   DATIAZ010000233 DATIAZ010000000
VERSION     DATIAZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63244"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VLF19_08275"
     CDS             complement(<1..613)
                     /locus_tag="VLF19_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93288.1"
                     /translation="MIWYALRRLVLAIPLLIGITFVSFLVIHLAPGDPVEFQTGELSP
                     ESGAQARQMLHELYGLDKPLHVQYWSWLTRLARFDFGRSFAPDGRPVLDKIRERLPVT
                     LALNIIELVIILLIAVPVGVLSATRQHSFFDKVTTVFVFVGFATPDFWLALLLMIFFG
                     VQLGWLPISGLRSLNWEYLPLWRQQWDFLSHLMLPIAVATFGGL"
     gene            complement(654..2297)
                     /locus_tag="VLF19_08280"
     CDS             complement(654..2297)
                     /locus_tag="VLF19_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HSE93289.1"
                     /translation="MRPLRPGALLLLAALAGGCGTDAGGAAEETPGTSGRSPAYGDTF
                     VEATIGAVSGLIPNITSDAASHEVGSLLYTGLVTRDRDLNIVGDMAESWEFSKDCLDL
                     TLRLRSGVKWHDGRPFTADDVVFTHQTMVHPKTPTAYKEDFNVVEAIEAPDPLTVRVR
                     YKRPHAKALQSWSTWILPRHVLEPYVLEGRLREASWLPPVGTGPYKLKEVRAAEKVVL
                     VANRDYYEAGPYISRLVYRVIPSQATIFLELKARGVDAASLTALQYKRQTEYPAFRKA
                     YQKFRYPSNSYTYFGFNLKDPRFADKRVRQAFAHAIDKGQLIDGVRLGLAREATGPYK
                     PGTWVYNPNVKRYPHDLAMARRLLAEAGWKEKNADGILVKDGKPFTFTLLTNQGNDER
                     KKIAEIIQASLREVGVGVDIKILEWASFIKEYVKKRRFEAIVLGWGIGQDPDQYNIWH
                     SSQTGPDQLNHVSFASAAVDELLERGRTSCHQAERKKYYDRIQEILAEEQPYVFLYFP
                     DALPTVARRVHNISESPNGIKYNFTEWYVPRQLQRYTAG"
     gene            complement(2297..2635)
                     /gene="secG"
                     /locus_tag="VLF19_08285"
     CDS             complement(2297..2635)
                     /gene="secG"
                     /locus_tag="VLF19_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSE93290.1"
                     /translation="MYIALVVIHVVACFAIMGMVLLQAGKGADIGSAFGGAGSQAVFG
                     SMGTPTVLGKITAGIAVVFVLTSFTLALLGGERSGSVIREAPPAPSQPAAPAPAPPAP
                     AQPAAPAPGK"
     gene            complement(2635..3417)
                     /gene="tpiA"
                     /locus_tag="VLF19_08290"
     CDS             complement(2635..3417)
                     /gene="tpiA"
                     /locus_tag="VLF19_08290"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSE93291.1"
                     /translation="MRTPLVMGNWKMHGTLAEARALATAVRDGLKRPRGVEVVLCPPF
                     TALAAVAEILGGGPIRLGAQNCHWEASGAHTGEIAPPMLVDVGCRYVIVGHSERRREM
                     GETDEQINRKVRAALAHGLTPVLCVGESAEERRQGLTFTTVEGQLRAGLSGMTAEAVA
                     RIVLAYEPVWAIGTGVNATPTQATEVHGYLRGLLSELTSKETAQTIRILYGGSVKAEN
                     AEALGAEPEIDGALVGGASLNAQGFGTIARKTARAGAVARGE"
     gene            complement(3449..4630)
                     /locus_tag="VLF19_08295"
     CDS             complement(3449..4630)
                     /locus_tag="VLF19_08295"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSE93292.1"
                     /translation="MAKLTIERLDLAGKRVFLRADLNVPLDGGVVGDDTRLRAVVPTI
                     QYALGHRAAVVLASHLGRPKGSPSPKYSLAPVAERLAAVLAQPVPLTPDCIGSEVMER
                     AHALKPGEVLLLENLRFHAEEEANDESFARGLAALADCYVNDAFAAAHRAHASIAAIT
                     RFLQPAAAGLLMQRELDALTRILERPERPLVAVLGGAKVSDKLALVEHLLDRVDSLVI
                     GGGMAFTFLRSLGHDVGRSLLEAEQIETARRVLEAARRRGTPVVLPVDAVVAAGLDSP
                     SGRAVSVREIPAAQMGLDIGPLTVERFAATLKSARTIVWNGPMGVFEKPAFARGTLDI
                     ARAVAAGQAFSVIGGGDTVAAVNQAGVADRIGYISTAGGAFLEFLEGRTLPGVAALTD
                     A"
     gene            complement(4642..5646)
                     /gene="gap"
                     /locus_tag="VLF19_08300"
     CDS             complement(4642..5646)
                     /gene="gap"
                     /locus_tag="VLF19_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSE93293.1"
                     /translation="MSTRVGVNGFGRIGRVFFRTAVNDQDIEVVGVNDLADAKTLAHL
                     LKHDSVHGNLRAEVAAKDGAIFVNGREIRVTAVKDPASLPWKELGVDVVVESTGVFRD
                     TATASKHLQAGAKKVIITAPAKDPDVTIVLGVNEQAYDARKHRILSNASCTTNCLATT
                     VKVLDDAFGVKRGFATTVHSYTNDQPVHDFPHKDLRRARAAAVSMIPTTTGAATAVGL
                     VLPKLKGRLDGIAIRVPTANVSVVDLVAELDTPASPGAVNEAFREAAGGKLRGILDAT
                     DEELVSVDFNGNPHSSIVDLPSTALVDGNLIKVLAWYDNEWGYSSRLRDLVRLVGASL
                     "
     gene            complement(5697..6515)
                     /locus_tag="VLF19_08305"
     CDS             complement(5697..6515)
                     /locus_tag="VLF19_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HSE93294.1"
                     /translation="MRPWVNAALDLLYPALCPLCDAVLGPGRRDPLCGACWTTMPRLA
                     PPLCPRCGASVGSFASPPESSRLSGGSPRTGTSVISPLGSPPEGGAPRTAICGGCLVE
                     PPAWDWGLAAAPYTGPVREALQALKFRGRRALAAPLGDLLVERCGPAFPDCVDALVPV
                     PLAPSRERARGFNQARLLADRAAPGFGRPVRPGWLARARPTAPQSDLDAEARRANVRG
                     AFRAAPAVAGCHVVVVDDVLTTGATVTECARALRAAGARGVGVVVVARVDALTL"
     gene            complement(6529..7473)
                     /locus_tag="VLF19_08310"
     CDS             complement(6529..7473)
                     /locus_tag="VLF19_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93295.1"
                     /translation="MPSLSFLTVAGRFLARIVGAAPLTGAEDPEASALVHRGDEAYRA
                     GRRDEARRCYLDALGRRKHDVDALRGLRTLAIDAGSWPEALGFAERVVAAAPAAARAA
                     ESGWLAAVQYELGRAESRRGQPQAAISHFRSALRADKGFLPAALALGEAWEAAGDPRE
                     AVRAWERALEHAPALPLLACLERAYRREGRPSRMIALYRSALERVPDDRALGVALGRV
                     YLELEMLDEAADQFEKVEVRAPDLPVVHAFLGAVFERRGEAREAFAEYRKALHLTGAF
                     DWPQRCAACGGTAPAWQDRCPGCGRWNTLRPVGDTSAG"
     gene            complement(7526..7891)
                     /locus_tag="VLF19_08315"
     CDS             complement(7526..7891)
                     /locus_tag="VLF19_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSE93296.1"
                     /translation="MRKDRLASFKKRLLEKQHQLMTEVGRTALYGKDQEDDSIKDLGD
                     QANTAYTREFFFELGNGDRRLLREVVTALQKIDDGSYGTCERCSEPIAEKRLDALPFA
                     RYCITCQRLVEEEERMATG"
     gene            complement(7968..8324)
                     /gene="rsfS"
                     /locus_tag="VLF19_08320"
     CDS             complement(7968..8324)
                     /gene="rsfS"
                     /locus_tag="VLF19_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSE93297.1"
                     /translation="MTGRPTAERKVRHAARAAQDKKAVDLVVLDVRALSAVADYFLVC
                     TGRSATHAATIADAVRHGLKELDVRPLHAEGLAQSGWILLDYGDVLVHVFLEETRDYY
                     ALERLWGDAPAVSLEG"
     gene            complement(8321..8971)
                     /gene="nadD"
                     /locus_tag="VLF19_08325"
     CDS             complement(8321..8971)
                     /gene="nadD"
                     /locus_tag="VLF19_08325"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HSE93298.1"
                     /translation="MARIAVFGGSFNPIHYGHLLLADEILEQLGLDRVLFVPAGVPPH
                     KAAASLAPAADRHAMVALAIAGHPRFDVSDVELRRSGPSYTVDTLETLATTGDELFLL
                     VGSETFLDLLSWRAPRRIATLARLVVVPRAGSAFDPESAAAQKVLREIGAERFTDAGS
                     RPAPPGTVVIAHVTSLPISASDLRRRAREGRSLAYRLPDPVIAHIAARRLYGGPAA"
     gene            complement(8976..>9359)
                     /locus_tag="VLF19_08330"
     CDS             complement(8976..>9359)
                     /locus_tag="VLF19_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSE93299.1"
                     /translation="CPRTRALVPGARPASEQDWDEEYLDLVLAVRVVDDLDDAIRHVR
                     RHGSGLAEAIVTNTLAHARRFTREVDAAVVLVNASTRFVDGQQFGMGAEMGISTSRVH
                     ARGPVGAQELTTTKYVVYGDGQVRE"
BASE COUNT         1353 a         3279 c         3427 g         1300 t
ORIGIN      
        1 cgaggccgcc gaaggtggcg accgcgatcg gcagcatcag gtggctcagg aagtcccact
       61 gctgccgcca gaggggcaga tactcccagt tcagggaccg cagccccgag atcggcagcc
      121 agccgagctg caccccgaag aagatcatca gcaggagcgc cagccagaaa tcgggcgtgg
      181 cgaacccgac gaagacgaag accgtcgtca ccttgtcgaa gaaggagtgc tgccgggtgg
      241 cgctgagcac gcccacgggg acggcgatga gcaggatgat caccagctcg atgatgttga
      301 gggcgagcgt gaccgggagg cgctcgcgga tcttgtcgag caccgggcgg ccgtcgggcg
      361 cgaaggagcg gccgaagtcg aaccgcgcca ggcgcgtgag ccagctccag tactggacgt
      421 ggaggggctt gtccaggccg tacagctcgt gcagcatctg cctggcctgc gcgccggact
      481 cgggactgag ctcgcccgtc tggaactcga ccgggtcgcc gggggcgagg tggatgacga
      541 ggaacgacac gaaggtgatc ccgatcagga gcgggatcgc gaggacgagg cggcgcagcg
      601 cgtaccagat catggggcgg gattatagtc ggggtcggcc ccgacggccg ggctcatccg
      661 gccgtgtagc gctgcagctg cctggggacg taccactccg tgaagttgta cttgatcccg
      721 ttcggcgact ccgagatgtt gtgcacgcga cgggcgaccg tcggcagcgc gtccgggaag
      781 tagaggaaca cgtacggctg ctcctcggcg aggatctcct ggatgcggtc gtagtacttc
      841 ttgcgctcgg cctgatgaca ggaggtgcgt ccgcgctcga gcagctcgtc caccgcggcg
      901 ctggcgaacg agacgtggtt gagctggtcg ggccccgtct gcgaggagtg ccagatattg
      961 tactggtccg ggtcctgacc gatcccccag ccgagcacga tggcctcgaa gcgtcgcttc
     1021 ttgacgtact ccttgatgaa ggacgcccac tcgaggatct tgatgtccac cccgaccccg
     1081 acctcgcgca gcgaggcctg gatgatctcg gcgatcttct tgcgctcgtc gttgccctgg
     1141 ttcgtgagca gcgtgaacgt gaagggcttg ccgtccttca ccaggatacc gtccgcgttc
     1201 ttctccttcc agccggcctc ggcgagcagg cggcgggcca tggccaggtc gtgcgggtac
     1261 ctcttgacgt tcgggttgta gacccaggtg ccgggcttgt acggccccgt ggcctcgcgc
     1321 gccagcccca ggcgcacgcc gtcgatgagc tggcccttgt cgatggcgtg cgcgaaggcc
     1381 tgccgcacgc gcttgtcggc gaatcgcgga tccttcaggt tgaagccgaa gtaggtgtag
     1441 gagttcgacg ggtaccggaa cttctggtac gccttccgga acgcggggta ctcggtctgg
     1501 cgcttgtact ggagcgcggt gaggctggcc gcgtcgacgc ccctggcctt gagctcgagg
     1561 aagatggtcg cctggctcgg gatcacccgg tacacgagcc gcgagatgta cggccctgcc
     1621 tcgtagtagt cgcgattagc gacgagcacc accttctccg ccgcgcgcac ctccttgagc
     1681 ttgtagggtc cggtgcccac cggcggcagc cagctggcct cgcgcagccg tccctcgaga
     1741 acgtagggct cgagcacgtg ccggggcagg atccaggtgg accagctctg cagcgccttg
     1801 gcgtgggggc gcttgtagcg cacgcgcacg gtgagggggt ccggcgcctc gatggcctcg
     1861 acgacgttga agtcctcctt gtacgcggtg ggcgtcttcg ggtgcaccat cgtctggtgc
     1921 gtgaagacca cgtcgtcggc agtgaacggg cgcccgtcgt gccacttcac gccggatctg
     1981 aggcgcagcg tgaggtccag gcagtccttg ctgaactccc acgactcggc catgtcgccg
     2041 acgatgttga ggtcgcggtc gcgcgtgacg agcccggtgt agaggaggct gccgacctcg
     2101 tgcgaggcgg cgtcgctggt gatgttggga atgagcccgg agaccgcgcc gatggtggcc
     2161 tcgacgaacg tgtcgccgta cgccggggac ctgccactgg tgcccggcgt ctcctcggcg
     2221 gcgccgccgg cgtcggtgcc gcagccgccg gcgagggcgg cgaggaggag cagcgcgcct
     2281 ggtcgaaggg ggcgcatcat ttccccggcg ccggcgcggc gggctgcgcg ggggccggcg
     2341 gggcgggcgc cggcgcggcc ggctgggagg gcgccggcgg cgcctcccgg atcaccgagc
     2401 cgctgcgctc accgccgagc aacgccaggg tgaacgaggt caggacgaag accacggcga
     2461 tgcccgccgt gatcttgccc agcaccgtcg gcgtgcccat ggagccgaac accgcctgac
     2521 tgccggcacc gccgaaggcg gagccgatgt cggcgccctt gcccgcctgg agcaggacca
     2581 tgcccatgat ggcgaaacac gcgacgacgt ggatcacgac cagcgcgatg tacatcactc
     2641 acccctcgcc acggcgccgg cccgcgcggt cttgcgcgcg atcgtgccga agccctgagc
     2701 attgagactg gcgcccccga cgagggcgcc gtcgatctcg ggctctgcgc cgagcgcttc
     2761 ggcgttctcg gccttgacgc tgccgccgta caggatgcgg atcgtctgcg cggtctcctt
     2821 cgaggtcagc tccgagagca gcccgcgcag gtagccgtgg acctcggtgg cctgggtggg
     2881 tgtggcgttg acgccggtgc cgatggccca cacgggctcg taggcgagca cgatcctggc
     2941 aacggcctcc gccgtcatgc cggacagccc cgcgcggagc tgaccctcga cggtcgtgaa
     3001 cgtgagccct tgacgccgtt cctcggcgct ctcgcccacg cacaggaccg gcgtgagccc
     3061 gtgggcgagc gccgcccgca ccttgcggtt gatctgctcg tcggtctctc ccatctcgcg
     3121 ccgccgctcg gagtggccga cgatgacgta gcggcacccg acgtcgacga gcatcggggg
     3181 ggcgatttcg cccgtgtgcg cgcccgacgc ctcccagtga cagttctgtg cgccgagacg
     3241 gatcggtccg ccgccgagga tctcggccac cgccgcgagc gccgtgaacg gcgggcacag
     3301 cacgacctct acgccccggg ggcgcttgag cccgtcgcgg accgcggtgg ccagcgcgcg
     3361 cgcctcggcg agcgtgccgt gcatcttcca gttccccatg accaggggcg tgcgcatcac
     3421 cgggctccgg ccatcgggca cgccggcatc aggcgtccgt cagcgcagcc acgccgggca
     3481 gggtccgccc ctcgagaaac tcgaggaaag ccccgcctgc ggtcgagatg tagccgatgc
     3541 ggtcggccac gccggcctga ttgacggcgg ccacggtgtc gccgccgccg atgacggaga
     3601 aggcctgtcc ggcagcgacg gcgcgcgcga tgtcgagcgt cccccgggcg aaggcgggct
     3661 tctcgaagac gcccatgggc ccgttccaca cgatggtgcg agcgctcttg agcgtggcgg
     3721 cgaacctctc gacggtcagc ggtccgatgt cgagccccat ctgcgccgcg gggatctcgc
     3781 gcacggacac cgcgcgcccg ctcggactgt cgagcccggc cgcgaccacc gcgtccaccg
     3841 gcagcacgac gggggtaccg cgccgccgcg ccgcctccag cacccggcgc gcggtctcga
     3901 tctgttcggc ctcgagcagc gaccgcccca cgtcgtggcc gagcgacctc aagaacgtga
     3961 aggccatgcc gcccccgatg acgaggctgt ccacgcggtc cagcaaatgc tcgaccagcg
     4021 cgagcttgtc ggacaccttg gcaccgccca gcacggcgac gagcggacgc tcggggcgct
     4081 cgaggatgcg cgtgagcgcg tcgagttcgc gctgcatcag gagcccggcc gccgcgggct
     4141 ggagaaagcg cgtgatggcc gcgatcgagg cgtgcgcgcg gtgtgcagcg gcgaacgcgt
     4201 cgttgacgta gcagtcggcg agggcggcca gcccgcgggc gaagctctcg tcgttggcct
     4261 cctcctcggc gtgaaaccgc agattctcca gcagcagcac ctcgccgggc ttcagcgcgt
     4321 gcgcccgttc catcacctcg gagccgatgc agtcaggagt cagggggacg ggctgggcga
     4381 gcaccgctgc gaggcgctcg gccaccggag ccagcgagta cttcggcgac ggcgagccct
     4441 tcggacgacc caggtgggag gccaggacga cggcggcgcg gtggccgagc gcgtactgga
     4501 tggtcggcac gacggcccgt aaccgcgtgt cgtcgccgac gacgccgccg tccagcggga
     4561 cgttgaggtc cgcgcggagg aagacgcgct taccggcgag gtcgagccgc tcgatcgtga
     4621 gcttcgccac gcgcggacgg ctcagagcga ggcgccgacg agccggacga gatcccgcag
     4681 gcgcgaggag tacccccact cgttgtcgta ccaggccagg accttgatca ggttgccgtc
     4741 gacgagcgcg gtggacggca ggtccacgat cgacgagtgc gggttgccgt tgaagtcgac
     4801 cgacacgagc tcctcgtcgg tggcgtcgag gatgccgcgc agcttgccgc cggccgcctc
     4861 gcggaacgcc tcgttgaccg cgccgggcga cgccggcgtg tccagctccg cgaccaggtc
     4921 gaccaccgag acgttcgccg tcggcacgcg gatggcgatg ccatcgagcc ggcccttgag
     4981 cttcggcagc acgaggccga ccgcggtggc ggcgccggtg gtcgtgggga tcatgctcac
     5041 cgcggccgcg cgggcccgac gcaggtcctt gtgggggaag tcgtggacgg gctggtcgtt
     5101 cgtgtaggag tgcacggtgg tggcgaaacc gcgcttgacg ccgaaggcgt cgtcgagcac
     5161 cttcacggtc gtggccaggc agttggtcgt gcacgaggcg ttggacagga tccggtgctt
     5221 cctcgcatcg taggcctgct cgttcacccc gagcacgatc gtgacgtcgg ggtccttggc
     5281 cggcgccgta atgatgacct tcttggcgcc cgcctgcaga tgcttcgatg ccgtcgccgt
     5341 gtcgcgaaag acgcccgtgg actcgacgac gacatccacc ccgagctcct tccacggcag
     5401 gctggccggg tccttcaccg cggtgacgcg gatctcgcgg ccattgacga agatcgcgcc
     5461 gtccttggcg gcgacctcgg cgcggagatt gccgtgcacc gagtcgtgct tgagcaggtg
     5521 ggccagcgtc ttggcatcgg ccaggtcgtt gacgccgacc acctcgatgt cctggtcgtt
     5581 cacggcagtc cggaagaaga cgcgtcctat ccggccgaag ccgttgacgc cgacccgagt
     5641 actcatggaa gggctccttg tgcgggctcc gagcgttgcc acctcgacac cgctgattat
     5701 agggtcagcg cgtccacgcg ggcaactacg acaaccccca ccccgcgggc gccggccgcg
     5761 cgcaacgccc gggcgcactc ggtgaccgtg gcgcccgtcg tcagcacgtc gtccacgacg
     5821 acgacgtgac agcccgccac cgccggcgcc gcgcggaacg caccgcgcac attggcgcgc
     5881 cgcgcctcgg cgtccaggtc gctctgaggc gccgtcgggc gtgcgcgggc gagccagccc
     5941 ggccggacgg gccggccgaa tcccggtgcg gcgcggtcgg cgagcagtcg cgcctggttg
     6001 aagccacggg cccgctcgcg ggacggcgcg agcgggacgg gcaccagcgc gtcgacgcaa
     6061 tccgggaacg cgggcccgca gcgctcgacg aggaggtcgc cgagcggcgc cgcgagcgcg
     6121 cgccggccgc ggaacttgag cgcctggagc gcctcgcgca ccggtcccgt gtagggggcc
     6181 gccgccagcc cccagtccca cgccggcggt tcgacgaggc acccaccgca gatggcagtg
     6241 cgcggtgcgc cgccctcggg cggcgagccg agcggtgaga tgaccgaggt ccctgtgcga
     6301 ggcgagccgc ccgacagcct cgaggactcc ggcggcgagg cgaacgaccc gaccgacgcc
     6361 ccacagcgcg gacacagcgg gggcgccagg cgcggcatcg tcgtccagca ggcgccgcac
     6421 agcgggtcgc ggcgccccgg gccgaggacg gcgtcgcaca gcggacacag cgctggatag
     6481 agaaggtcca gcgcggcgtt gacccacggg cgcatcggcg ggggcggctc agcccgcgga
     6541 ggtgtcgccg accggacgca gggtgttcca gcggccgcag cctgggcacc ggtcctgcca
     6601 ggccggtgcg gtgccaccgc acgccgcgca gcgctgcggc cagtcgaacg cgccggtcag
     6661 gtgcagcgcc ttgcggtact cggcgaacgc ctcgcgcgcc tcgccgcgcc gctcgaagac
     6721 cgcgccgagg aacgcgtgca ccaccggcag atccggagcg cgcacttcga ccttctcgaa
     6781 ctggtcggcc gcctcgtcca gcatctcgag ctcgaggtac acccgtccca gagccacccc
     6841 gagcgcccga tcgtccggga cgcgctcgag cgccgagcgg tagagcgcga tcatgcggct
     6901 cggccggccc tcgcggcggt acgcccgctc gaggcaggcg agcaggggca acgccggcgc
     6961 gtgctcgagc gcgcgctccc acgcccgcac ggcctcgcgc ggatcgccgg ccgcttccca
     7021 cgcctcgccg agcgcgagcg cggcgggcag gaatcccttg tcggcgcgca gcgcgctgcg
     7081 gaagtgagag atcgccgcct ggggctggcc gcgtcgagac tccgcgcgcc cgagctcgta
     7141 ctgcaccgcg gccagccagc cggactcggc ggcccgcgcc gcggcgggtg ccgccgcgac
     7201 cacgcgctcg gcgaacccga gcgcctccgg ccacgacccc gcgtcgatcg cgagcgtgcg
     7261 cagcccgcgg agcgcgtcga cgtcgtgctt gcgacggccg agcgcgtcga gatagcagcg
     7321 ccgtgcctcg tcgcggcgac ccgcgcggta cgcctcgtcg ccgcggtgga cgagcgccga
     7381 cgcctcggga tcctccgcgc ccgtgagggg ggcggcgccg acgatgcggg cgagaaaccg
     7441 cccggccacg gtcaggaaac tcagcgacgg catgcgagga cgcgaggggg ccggaggcgg
     7501 cgcgcggcgg aaccggcccg ggcggctagc ccgtggccat gcgctcttct tcctcgacca
     7561 gtcgctggca ggtgatgcag taacgcgcga acggcagcgc gtccaggcgc ttctcggcga
     7621 tcggctcgct gcagcgctcg caggtgccgt acgagccgtc gtcgatcttc tgcagcgcgg
     7681 tgacgacctc gcgaagcaac cggcggtcgc cgttgccaag ctcgaaaaag aactcgcgcg
     7741 tataggcagt gttcgcctga tcgccgaggt ccttgatgga gtcgtcctcc tggtcctttc
     7801 catacagggc cgtgcggcct acctcggtca tcagctggtg ctgcttctcg agcaggcgct
     7861 ttttgaagga cgccagccgg tcttttctca tcgggacctc cagtgtctcc cgcgtgccca
     7921 ggtacgctag catcgaccga caccccgtgc aagaccctct cccgccttca accctcgagc
     7981 gacaccgccg gcgcgtcgcc ccagagccgc tcgagcgcgt agtagtcgcg cgtctcctcg
     8041 aggaagacgt gcacgagcac gtcgccgtag tcgaggagga tccagccgct ctgcgcgagg
     8101 ccttcggcat gcagcggccg gacgtcgagt tccttgagcc cgtggcgaac ggcgtcggcg
     8161 atcgtcgccg cgtgcgtggc cgagcggccg gtgcagacga ggaaatagtc ggcgacggcg
     8221 ctcagtgccc ggacgtcgag cacgacgaga tcgacggcct tcttgtcctg cgcggcgcgc
     8281 gccgcgtggc ggaccttccg ctcggcggtc gggcgccccg tcacgcggcc gggcccccgt
     8341 agaggcgccg cgccgcgatg tgcgcgatca ctggatcggg caggcggtac gcgagcgagc
     8401 gcccttcgcg cgcccggcgc cgcaggtccg aggcggagat cggcagcgag gtcacgtgcg
     8461 cgatcacgac cgtgccgggg ggggccggcc ggctcccggc gtccgtgaat cgctcggcgc
     8521 cgatctcgcg cagcaccttc tgggccgcgg cgctctcggg atcgaacgcg ctgcccgcgc
     8581 gcggcaccac gaccagccgc gcgagggtcg cgatgcgccg aggcgcacgc cacgacagca
     8641 ggtcgaggaa cgtctcggag cccacgagca ggaacagctc gtccccggtg gtggccagcg
     8701 tctccagcgt atccacggtg taggacgggc ccgagcgccg gagctccacg tccgagacgt
     8761 cgaagcgtgg atgaccggcg atggccaggg cgaccatggc gtggcggtcg gccgccgggg
     8821 cgagcgaggc ggccgccttg tggggcggca cgccggccgg cacgaacagg acgcggtcca
     8881 ggccgagctg ctccaggatc tcgtcggcga gcagtaggtg gccgtagtga atcgggttga
     8941 acgacccgcc gaacaccgcg atgcgggcca cccggctact cgcgcacctg gccgtcgccg
     9001 tagacgacgt atttcgtggt tgtcagctct tgcgcgccga ccgggccgcg cgcgtgcacg
     9061 cgggacgtgg agatgcccat ttcggcgccc atcccgaact gttgaccgtc cacgaatcgc
     9121 gtcgaggcat tgaccaggac caccgcggcg tccacctcgc gcgtgaagcg gcgcgcgtgg
     9181 gcgagcgtgt tcgtcacgat tgcctcggcg agccccgagc cgtggcggcg gacgtggcgg
     9241 atcgcgtcgt cgaggtcgtc cacgacgcgg accgcgagga cgaggtcgag gtactcctcg
     9301 tcccagtcct gctcgctcgc cggccgcgcg cccggtacca gggcgcgcgt gcgcgggca
//
LOCUS       DATIAZ010000234         8420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_63498, whole genome shotgun sequence.
ACCESSION   DATIAZ010000234 DATIAZ010000000
VERSION     DATIAZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63498"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VLF19_08335"
     CDS             <1..823
                     /locus_tag="VLF19_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HSE93300.1"
                     /translation="RQFVRGLRAGALEEQGCYDGAPMPVQTLHALQNGFMGAPRSLLF
                     YGEYSGETVQIPVACYVIRTGDAVMLFDTGLSPRAIPGLCRNDPLARFTTEDLLVHRL
                     DGLGLEVGDVDLVVLSHLHYDHAGGAELFPDAELCVQRDEHAYAHNPAPFFAPFYYRK
                     NFDLPRARWRLLDGDAELAPGVTALRTDGHTPGHQSLLVELPASGPVILTGDACYWQE
                     HFDAERVPGVVWSPTLAFHSIKRLKTLARLTGARVFPGHDPKFWRTVRQAPDAYR"
     gene            857..1849
                     /locus_tag="VLF19_08340"
     CDS             857..1849
                     /locus_tag="VLF19_08340"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HSE93301.1"
                     /translation="MASVIVSALLVLVVALTAVPPAAGQAREMTMGNVNPPKHGTSQA
                     SQQFIDKLAELTGGKTKVVHHHSGALGGEREVAQQIQLGTVDFGPITTAPLSTLVPEM
                     SVFQLPYIFRDYQHVYQALDGSDVIRKYYEGVLDKKGFRLVGFIAAGYRGIYGHYPIN
                     SIADVKGKKIRVQEDRILVATFKALGMISTPIAFPEVATALQTRVIDFAEGGINTYYH
                     NKFYDIVKNVADVRHTHQCVALVMSKASWQKQDAAARKAIGEAWEHARQFNRKFILDE
                     DKSIQDQVREKGATITKPDATPFREATRSVYEEFFATPAGKDARKVVDHILGIK"
     gene            1852..2322
                     /locus_tag="VLF19_08345"
     CDS             1852..2322
                     /locus_tag="VLF19_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSE93302.1"
                     /translation="MRRLYEGLALLGGLVLAAMTGAVFLQVVLRYALNTGLEGLDEVP
                     RYLFVWLVMLGAAAAMYRGEHTALEFFRGRLPPRGQALVRVVTNGAAVALFLSLIHSS
                     LVLVPNAQLQSSPGLGLPLGYVYAAVPVGSVLIILPLVGNVVRAVRELLWPRPF"
     gene            2304..3590
                     /locus_tag="VLF19_08350"
     CDS             2304..3590
                     /locus_tag="VLF19_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HSE93303.1"
                     /translation="MAETLLIVFVVLVALGTPIGMALGAGALVAALLYPALNPIIIPT
                     RFVGLLSDSFLILAAPLFIRAGNIAARGGVARAIIDLATVLVGRFRGGLAYVNIVDSM
                     IFGGISGSAVADVSALGTFLVPQMVRKGYDKDFATVLTICTAILAPIVPPSIVAVIYA
                     WMADESVAAIFAGGYLPGLLIGLGMAVPTFVIARRRNYPREPRPTLATFTAALRQALP
                     GLMIPVIIMGGILAGLFTPTEAAAVAVVYALLVPPLFYRELAWRDLPVIFADSARLSG
                     VIGLIIGLVGAFGWVLTYSKFPFRVAEAIQTFAPTWWLFLSFVIVLYVFLGTFLTPSE
                     IILVTVPVLLPGAQALGIHPIHFGMVCVIASAIGHVTPPVGLCLFLGMAISGLPMEKL
                     VKPLVPFLAAIVIVLFLVAFVPETVLLLPRWLGLVK"
     gene            complement(3597..3800)
                     /locus_tag="VLF19_08355"
     CDS             complement(3597..3800)
                     /locus_tag="VLF19_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HSE93304.1"
                     /translation="MAWRYGRKAGKTVERAMHKRKRGRLKSGRSGKTVRSRKQALAIG
                     LSEARKKGAKVPRRRGRRRRKTS"
     gene            4023..4553
                     /locus_tag="VLF19_08360"
     CDS             4023..4553
                     /locus_tag="VLF19_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE93305.1"
                     /translation="MGQILTECCMEIRMLSAPDTDAWLRLRVRALRDHPDAFGRAAEE
                     ADTAEEWRRRFETNENGREGFLLGAFEVGLVGAVGCQRARGQRVRHAAMLWGMYVAPE
                     ARRRGIGAALLDAAIARARAWPDLDHLTLGVMRHQTPARRLYLSRGFGTTGCHPRALR
                     VGDAYYDEEQMLLRLG"
     gene            4550..5257
                     /locus_tag="VLF19_08365"
     CDS             4550..5257
                     /locus_tag="VLF19_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSE93306.1"
                     /translation="MTVTERVAALDWDALERSLWSWGYAKTPPLLTSDECATLVTLYA
                     DDSRFRSHIDMARYRFGVGDYKYFAEPLPPIVAELRLRFYPPLAAVANGWEQALGSRR
                     RYPPDLEGLKVQCRRAGQTKPTPLLLHYEAGGYNCLHQDLYGDVAFPLQLTCFLSRPG
                     SDYHGGEFLLVEQRPRAQSRGEAITTAQGEIVIFATRHRPAAGARGYHRVVMRHGVSR
                     VHAGSRYTLGIIFHDAR"
     gene            5391..6764
                     /locus_tag="VLF19_08370"
     CDS             5391..6764
                     /locus_tag="VLF19_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HSE93307.1"
                     /translation="MSSSARLAEFVVKSTLDDCPPEALLRVRRAALDSLGVMLAGARE
                     PVARIVREVARAEGGVPLCTVVGSSLRTAPGWAALANGAAGHAHDFDDTSFALLGHPS
                     VPLLAALLAAGEAEMADGRAVALAYVLGFEVSAAIGAAINPAHYSRGWHATISVGTFG
                     CAAAAARVLALDVVQTTHALGLAASLASGLKENFGSMTKPYHAGQAALNGVRAAQLAR
                     QGLTASGRALDGPQGYLAVMAEPAAAAALAAALEGLGRRWHIVDTGVAVKPYPSCALT
                     HSAIDALVELRHEHRIRPEQVASVEVAVNRVTPTVLVHARPATALERKFSMPYCAAAA
                     LAEGRVDFASFEDGEVRDAAVRGLMERVSMVVDEGLGERLEEQAWSRVTVRLADGRVL
                     ACPPRGAAGHPDRPLSDEALREKFLACALAPLGRGEAEGVAEQVRHLEDIPDIRLLTS
                     RLTGALE"
     gene            6772..7965
                     /locus_tag="VLF19_08375"
     CDS             6772..7965
                     /locus_tag="VLF19_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSE93308.1"
                     /translation="MSALDGIKIIELARVPPAEMPGMMLADMGADVLKIETPEPGRQR
                     DELSDRRAAFAFVNRNKRSMALNLKAPEGQAILRKLVAGADVVVEGFRPGVMKRLGAD
                     YDTVRTLNPRIVYCSLSGFGQDGPYRDFPAHDMNYLSLAGVLGLIGEPDRKPVIPLNL
                     VADYAGASLHGALGIVLALFARERTGRGQHVDVSYLDTTVSLLAATPNMRFFFSDGVA
                     PRRGEGFLGGSYPYYAIYETRDGKLLTIGCTEPWLWENFCRAIERPDLARFARQADQF
                     VRAANAEEEAARREIEAIIRTRDRDDWYERLVKADVCVGKVYDVDEMVRDPQINHRGM
                     IVEAEHPRYGKVRQFGVAIKLSETPGIVRSAAPLAGEHTEAVLKDLGLGPEDIGALRA
                     KGVIE"
     gene            7980..>8420
                     /locus_tag="VLF19_08380"
     CDS             7980..>8420
                     /locus_tag="VLF19_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSE93309.1"
                     /translation="MVIGSGPAGEKGAAQAAYFGKRVALVERAPYLGGAGVNTGTIPS
                     KTLRESALYFSGLTQRGLYGRDYTLRENLTVQDFLYRQQEVVRALREVVLRNIERHHI
                     ELVHGEACLLDGHTVHVALATGGERRLAGAVILVATGSVPNRARD"
BASE COUNT         1135 a         3145 c         2824 g         1316 t
ORIGIN      
        1 gcggcaattc gtccgcggac tccgggccgg ggcgctcgag gaacagggat gctatgatgg
       61 cgcgccgatg ccggtccaga cacttcacgc gctccagaac ggcttcatgg gcgccccacg
      121 ttcgctgctc ttctacggcg aatactccgg cgagaccgtg caaatcccgg tcgcctgcta
      181 cgtgatccgc acgggtgacg ccgtgatgct cttcgacacc ggcctctcac cgcgcgcgat
      241 ccccgggctc tgccgcaacg acccgctggc gcgcttcacc accgaggatc tgttggtcca
      301 ccgcctcgac gggctcggtc tcgaggtggg cgatgtggac ctcgtcgtgc tctcgcacct
      361 gcactacgac cacgccggcg gcgcagagct gtttcccgac gccgagctgt gcgtgcagcg
      421 cgacgagcac gcctacgccc acaacccggc gccgttcttc gcacccttct actaccgcaa
      481 gaacttcgac cttccccgcg cgcgctggcg cctgctcgac ggcgacgcgg agctcgcgcc
      541 cggcgtcacc gccctgcgca ccgacggcca cacgccgggc caccagtcgc tgctcgtcga
      601 gctgccggcg agcgggccgg tgatcctcac cggcgacgcc tgttactggc aggagcactt
      661 cgacgccgag cgcgtgcccg gcgtcgtgtg gagcccgacg ctcgcgttcc actcgatcaa
      721 gcgcctcaag accctggcac gcctcaccgg cgctcgcgtc ttccccgggc acgacccgaa
      781 gttctggcgc acagtgagac aggcccctga cgcgtaccgc tgaccgccgg caagcgtcgg
      841 cgcaggagga ctccgcatgg catccgtgat cgtgagcgcg ctgctcgttc tcgtcgtcgc
      901 gttgaccgcc gtccctcccg ccgccggcca ggcacgcgag atgacgatgg gcaatgtcaa
      961 cccgccgaag cacggcacct cgcaggcgtc ccagcagttc atcgacaagc tcgccgagct
     1021 gacgggcggc aagacgaagg tcgtccacca tcactccggc gccctcggcg gcgagcggga
     1081 ggtcgcccag cagatccagc tcggcacggt cgacttcggc ccgatcacga cggcgcccct
     1141 cagcacgctc gtccccgaga tgtcggtctt ccagctccct tacatcttcc gcgactacca
     1201 gcacgtctac caggcgctcg acggcagcga cgtcatccgc aagtactacg agggcgtgct
     1261 cgacaagaag ggcttccggc tcgtcggctt catcgccgcg gggtaccgcg gcatctacgg
     1321 gcactacccg atcaactcga tcgccgacgt caagggcaag aagatccggg tccaggagga
     1381 ccggatcctc gtggcgacgt tcaaggcgct cggcatgatc tccacgccga tcgccttccc
     1441 ggaggtggcc acggcgctgc agacgagggt catcgacttc gccgagggcg ggatcaacac
     1501 ctactatcac aacaaattct acgacatcgt gaagaacgtc gccgacgtgc gccacaccca
     1561 ccagtgcgtc gcgctcgtca tgtcgaaggc ctcctggcag aagcaggacg ccgccgcccg
     1621 gaaggccatc ggcgaggcgt gggagcacgc ccgccagttc aaccggaagt tcatcctcga
     1681 cgaggacaaa tcgatccagg accaggtccg ggagaagggc gcgaccatca ccaagcccga
     1741 cgccacgccg ttccgcgagg cgacccgcag cgtctacgag gagttcttcg cgacaccggc
     1801 cggcaaggac gcacggaagg tcgtcgacca catcctcggc atcaagtagc gttgcgccgc
     1861 ctgtacgaag gcctcgcgct cctcggcggt ctcgtgctgg ccgccatgac tggcgcggtc
     1921 ttcttgcagg tggtcttgcg ctacgccctc aacacgggcc tcgaggggct cgacgaggtc
     1981 ccccgctacc tcttcgtctg gctggtcatg ctgggggccg ccgccgcgat gtaccgcggc
     2041 gagcacacgg ccctcgagtt cttccggggc cgcctgccgc cccgcgggca ggcgctggtc
     2101 cgggtggtga ccaacggagc ggccgtcgcg ctgttcctgt cgctcatcca ctcgagcctg
     2161 gtgctcgtcc cgaacgccca gctccagtcc agccccgggc tcggcctgcc gctcggctat
     2221 gtctacgccg ccgtgcccgt cgggtcggtg ctcatcatcc tgcccctggt cggaaacgtc
     2281 gtccgcgccg ttcgcgagct actatggccg agacccttct gatcgtcttc gtcgtgctcg
     2341 tggccctggg gacgccgatc ggcatggcgc tcggcgccgg cgcgctcgtg gcggcgctcc
     2401 tctacccggc gctgaacccg atcatcatcc cgacgcgctt cgtcggcctc ctgtccgact
     2461 cgttcctgat cctcgcggcg ccgctgttca tccgggccgg caacatcgcc gcgcgcggcg
     2521 gcgtcgcccg ggccatcatc gacctggcca cggtgctggt cggccgcttc cggggcgggc
     2581 tcgcctacgt caacatcgtg gacagcatga tcttcggcgg gatctcgggc tcggccgtgg
     2641 ccgacgtgtc cgcgctcggc accttcctgg tcccccagat ggtgcggaag ggttacgaca
     2701 aggacttcgc gacggtgctg acgatctgca ccgcgatcct cgcccccatc gtcccgccgt
     2761 cgatcgtggc cgtgatctac gcatggatgg ccgacgagtc ggtggcggcc atcttcgctg
     2821 gcggctacct gccggggctg ctgatcggcc tcgggatggc cgtgcccacg ttcgtcatcg
     2881 cgcgccggcg caactacccc cgcgagcccc ggccgacgct ggcgaccttc acggcggcgc
     2941 tccggcaggc gctgccgggc ctcatgatcc ccgtcatcat catgggcggc atcctcgccg
     3001 gcctcttcac cccgaccgag gccgccgccg tcgccgtcgt gtacgcgctg ctcgtgccgc
     3061 ccctcttcta ccgggagctc gcatggcgcg acctgcccgt catcttcgcc gactccgcgc
     3121 gcctgtcggg ggtgatcggc ctcatcatcg ggctcgtggg cgccttcggc tgggtgctga
     3181 cgtactcgaa gttcccgttc cgcgtcgccg aggcgatcca gacgttcgcc ccgacgtggt
     3241 ggctcttcct ctcgttcgtc atcgtcctct acgtcttcct gggaacgttc ctcacgccct
     3301 cggagatcat cctcgtcacc gtcccggtcc tgctgcccgg cgcgcaggcg ctcggcatcc
     3361 acccgatcca cttcggcatg gtgtgcgtca tcgcctccgc catcggacac gtcacgccgc
     3421 cggtcgggct ctgcctcttc ctcggcatgg cgatcagcgg cctgccgatg gaaaagctcg
     3481 tcaagccgct ggtacccttc ctcgccgcga tcgtgatcgt gctcttcctc gtcgccttcg
     3541 tgcccgagac agtgctgctc ctgccgcgct ggctgggcct ggtgaagtag gagatcctac
     3601 gacgtcttcc tccggcgccg cccccgccgc cgcggcacct tcgcgccctt cttgcgcgcc
     3661 tccgagagcc cgatggcgag cgcctgcttg cggctcctga ccgtcttgcc gctgcggcca
     3721 ctcttgaggc ggccacgctt cctcttgtgc atcgcgcgct cgaccgtctt gccggctttc
     3781 cgtccatacc gccaggccat gtgaccctcc tatgtgagca tgctcacccc gccaatggcc
     3841 atcccccgac gctcggcacg ccccgccggg gcctgacgca cctcgatctc cccgtacagt
     3901 ctcggcggtg ggcaggggtg cgcaccttcg ccctgctgtg caagctccgt gctgtcgtct
     3961 cgccgagccg acgggttcga ccgcatggcg acggcgatcg cctcggcgcg gccggtgcta
     4021 cgatgggcca gatcctgacg gagtgctgca tggagatccg gatgctgagc gcccccgaca
     4081 ccgacgcctg gctgcgcctg cgggtgcgtg cgctgcgcga tcatcccgat gccttcggcc
     4141 gggctgcgga ggaggcggac accgccgaag agtggcggcg ccggttcgag acgaacgaga
     4201 acgggcgcga ggggttcctg ctcggcgcct tcgaggtcgg tctggtcggc gccgtcggct
     4261 gtcagcgggc gcgcgggcag agggtgcgcc acgcggcgat gctctggggc atgtacgtcg
     4321 cgcccgaggc gcgccgccgg ggcatcggcg cggcgctgct cgacgcggcg atcgcgcgcg
     4381 ctcgggcctg gccggacctc gaccacctca cgctcggcgt catgcgacac cagacgcccg
     4441 cccgccgcct ctatctctcc cgcggcttcg ggaccacggg ctgccatccg cgcgcgctcc
     4501 gcgtcggcga cgcctactac gacgaggagc agatgctgct ccggctcgga tgaccgtcac
     4561 cgagcgggtc gcggccctcg actgggacgc cctcgagcgg tcgctgtggt cgtggggcta
     4621 cgccaagacc ccgcccctcc tgacctcgga cgagtgcgcc accctggtca ccctctacgc
     4681 cgacgactcg cgcttccgga gccacatcga catggcccgc tatcggttcg gcgtcggcga
     4741 ctacaagtac ttcgcggagc ccctgccacc gatcgtggcc gaactgcggc tgcgcttcta
     4801 cccgccgctg gcggccgtgg ccaacggttg ggagcaggcc ctcggctcgc ggcgccgcta
     4861 cccgcccgat ctggaaggcc tgaaggtcca gtgccggcgc gccggccaga cgaagcccac
     4921 gccgctcctg ctccactacg aagctggggg ctacaactgc ttgcatcagg acctgtacgg
     4981 ggacgtggcc ttccccctgc agctcacctg cttcctcagc cgaccggggt cggactacca
     5041 cgggggcgag ttcctgctcg tggaacagcg gccgcgcgcc cagtcccggg gcgaggccat
     5101 caccacggcg caaggcgaga tcgtgatctt cgcgacgcgt catcgcccgg cggccggcgc
     5161 ccgcggctat catcgcgtgg tcatgcgtca cggggtgagc cgcgtgcacg ccggttcgcg
     5221 ctacacgctc ggcatcatct ttcacgacgc gcgctgagcg cgggcggcgg cgacccccac
     5281 gacatctccc gcgtgggcac cgggcgcacg acgtcgttca tgccgccgcc ggacggcacg
     5341 gcgcgacaca ggggattgca gttggccgac ggctccgata aggtgcggtc gtgagctcga
     5401 gcgcccggct cgccgagttc gtcgtcaagt ccacgctgga cgactgcccg cccgaggccc
     5461 tcctccgggt ccgccgcgcg gcgctcgact cgctcggggt gatgctggcc ggcgcgcggg
     5521 agcccgtcgc gcgcatcgtg cgcgaggtcg cgcgcgccga gggcggggtg ccgctgtgca
     5581 cggtggtggg gtcgtccctg cgcaccgcgc ccggctgggc ggcgctcgcc aacggcgcgg
     5641 ccgggcacgc gcacgacttc gacgacacgt ccttcgccct gctggggcac ccgagcgtcc
     5701 ccctgctggc cgccctgctg gccgcgggcg aagccgagat ggcggatggc cgggccgtcg
     5761 cgctcgccta tgtcctgggc ttcgaggtct ccgcggcgat cggcgccgcg atcaaccccg
     5821 cgcactactc gcgcggctgg cacgccacca tcagcgtggg aaccttcggc tgcgccgctg
     5881 ccgccgcacg cgtgctcgcg ctcgacgtgg tgcagacgac gcacgccctc ggtctggccg
     5941 cctcgctggc gtccggcctc aaggagaact tcggctcgat gacgaagccg taccacgcgg
     6001 gccaggcggc gctgaacggc gtccgggcgg cgcagctcgc gcgccagggg ctgaccgcct
     6061 cggggcgcgc cctcgacggg ccgcagggct acctggccgt gatggccgag cccgcggccg
     6121 ccgccgcgct cgcggcggcg ctggaggggc tcggccgccg ctggcacatc gtggacacgg
     6181 gcgtcgccgt caagccgtat ccctcgtgcg cgctcaccca ctccgccatc gacgcgctgg
     6241 tcgagctacg tcacgagcac cggatccgtc cggagcaggt cgcttcggtg gaggtcgccg
     6301 tgaaccgcgt gacgccgacc gtgctcgtcc acgcacggcc ggcgacggcg ctcgagcgca
     6361 agttttccat gccatactgc gcagccgccg cgctggccga gggacgcgtg gacttcgcct
     6421 ccttcgaaga cggcgaggtg cgcgacgcgg cggtgcgcgg gctcatggag cgcgtgagca
     6481 tggtggtcga cgaggggctc ggcgagcggc tcgaagagca ggcatggagc cgggtgacgg
     6541 tgcgtctggc cgacggccgc gtgctggcct gtcctccacg cggcgccgcc ggccaccccg
     6601 accggccgct ctcggacgag gcgctccgcg agaagttcct cgcctgcgcg ctggccccgc
     6661 tcggtcgcgg tgaggccgag ggggttgccg agcaggtccg gcacctcgag gacatccccg
     6721 acatccgcct gctcacctcg cgcctgaccg gcgccctcga gtgacgcgct gatgagcgcc
     6781 ctcgacggga tcaagatcat cgagctggcc cgcgtgccgc ccgcggaaat gcccgggatg
     6841 atgctcgccg acatgggcgc cgacgtgctg aagatcgaga cgcccgagcc cggccgccag
     6901 cgcgacgagc tgtcggaccg gcgcgcggcc tttgccttcg tcaaccgcaa caagcgctcg
     6961 atggcgctga acctcaaggc gccggagggc caggcgattc tccggaagct cgtcgccggc
     7021 gccgacgtcg tcgtcgaggg cttccggccc ggcgtcatga agcggctcgg ggcggactac
     7081 gacacggtgc ggacactcaa tccacgcatc gtctattgct ccctgtccgg attcggacag
     7141 gacggcccct accgcgattt cccggcacac gacatgaact atctgtccct cgccggcgtg
     7201 ctcgggctca tcggcgagcc ggatcgcaag cccgtcatcc cgctcaacct cgtcgccgac
     7261 tacgcggggg cgagcctcca cggcgcgctc ggcatcgtgc tcgcgctctt cgcacgcgag
     7321 cgcaccgggc ggggccagca cgtcgacgtc tcgtatctcg acaccaccgt ctccctgctg
     7381 gcggcgacgc cgaacatgcg cttcttcttc agcgacggcg tggcgccgcg gcgcggcgaa
     7441 ggcttcctcg gtggctctta cccgtactac gcgatctacg agacgcgtga cggcaagctg
     7501 ttgacgatcg gctgtacgga gccctggctc tgggagaact tctgccgcgc catcgagcgg
     7561 cccgacctcg cgcgcttcgc gcggcaggcc gaccagttcg tccgcgccgc caacgccgag
     7621 gaggaggccg cgcgtcggga gatcgaggcg atcatccgga cgcgcgaccg ggacgactgg
     7681 tacgagcggc tcgtgaaggc cgacgtgtgc gtcggcaagg tgtacgacgt ggacgagatg
     7741 gtgcgcgacc cgcagatcaa ccaccggggc atgatcgtcg aggccgagca cccgcggtac
     7801 ggcaaggtgc gtcagttcgg cgtcgccatc aagctgtcgg agactccggg gatcgtccgc
     7861 agcgcggctc cgctggcggg cgagcacacc gaggccgtcc tgaaggacct cgggctcggg
     7921 cccgaggaca tcggcgcgct ccgcgccaag ggggtcatcg aatagaccgc tacgacctcg
     7981 tggtgatcgg gtcggggccg gcgggcgaga agggcgccgc tcaggcggcg tacttcggca
     8041 agcgcgtggc cctcgtggag cgcgcgccgt acctcggcgg cgccggtgtc aataccggca
     8101 ccattccttc gaagaccctg cgggagagcg cgctgtactt ctccggtctc acccagcgcg
     8161 gcctctacgg ccgggactac accctgcgcg agaacctgac cgtccaggac ttcctctacc
     8221 ggcagcagga ggtcgtccgc gccctgcgcg aggtggtcct ccgcaacatc gagcggcacc
     8281 acatcgagct cgtccacggc gaggcctgcc tcctggacgg gcacaccgtg cacgtggcgc
     8341 tggccacggg tggcgagcgc cggctggccg gcgcggtgat cctcgtggcc accggctcgg
     8401 tgccgaaccg ggcgcgcgac
//
LOCUS       DATIAZ010000235         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1115296, whole genome shotgun sequence.
ACCESSION   DATIAZ010000235 DATIAZ010000000
VERSION     DATIAZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115296"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VLF19_08385"
     CDS             <1..661
                     /locus_tag="VLF19_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93310.1"
                     /translation="VTFDVAPGELVGIIGPNGAGKSTLFDLVTGFARPDAGRVAVDGR
                     DVTRLRPDEICRLGVTRTFQKLRPFAGMTALENVMVGAFQRTGDVGEARDIASDTLAR
                     VGLADRAEAHARGLSTGQRKRLELARALATGPRLLLLDEVTGGVDQGAIPGLVALVRE
                     LHARGLAVLVIEHNMRVIMALAQRVVALQLGEVIADAPPAAIVRNARVIDAYLGQAWR
                     P"
     gene            691..1395
                     /locus_tag="VLF19_08390"
     CDS             691..1395
                     /locus_tag="VLF19_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93311.1"
                     /translation="MLEVRALDVVLGDYQVLWGAELRVDEGEIIAVLGPNGAGKSTLM
                     NTVSGLLAPRAGEVVFRGHPITGRPAHLVVREGLGHVLERRRLFPYLTVRQNVLLGAY
                     HPAARVGRDAQYDRMGALFPIIPARADQLAHTLSGGEQQMVAIARGLMARPRLLMIDE
                     PFLGLAPRVVDQIAEVIHRINADGVTVVFIEQNVERALGLAHRGYVLESGRTVLDGRS
                     GDLLRSAEVRRIFLGG"
     gene            1413..1766
                     /locus_tag="VLF19_08395"
     CDS             1413..1766
                     /locus_tag="VLF19_08395"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSE93312.1"
                     /translation="MADDKVETIVSRSSGTIGRAVSEARGARLTLDSSTHPKPDALTN
                     SEAFLAGVSSCGVTLIEAHAQQTGVPLTRMEVTIEGVRAAADPSRFSRVTMRFALAGV
                     SQAQAEDLVRTYQGR"
     gene            complement(1884..>2075)
                     /locus_tag="VLF19_08400"
     CDS             complement(1884..>2075)
                     /locus_tag="VLF19_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSE93313.1"
                     /translation="GEEVAAFVALREGARVSPDDLVAWCRERLAAFKYPRRVVVLDRL
                     PRSATGKILKARLIADRAP"
BASE COUNT          274 a          750 c          770 g          281 t
ORIGIN      
        1 cgtcacgttc gacgtggcgc cgggcgagct ggtcgggatc atcggcccca acggcgccgg
       61 gaagtcgacc ctcttcgacc tcgtcacggg cttcgcccgg cccgacgcgg gccgcgtggc
      121 cgtggacggg cgcgacgtca cgcggctgcg accggacgag atctgtcggc tgggcgtcac
      181 acgcacgttc cagaagctcc ggcccttcgc gggcatgacg gcgctcgaga acgtgatggt
      241 cggcgccttc cagcgcaccg gtgacgtcgg cgaagctcgt gacatcgcca gcgacacgct
      301 cgcgcgcgtc ggcctcgccg accgcgccga ggcgcacgcc cgggggctgt cgaccggaca
      361 gcgcaagcgg ctcgagctcg cccgggcgct ggcgacgggc ccgcgcctgc tgctgctcga
      421 cgaggtcacg ggcggggtcg accagggcgc catccccggg ctcgtggccc tcgtgcgcga
      481 gctgcacgcg cgcgggctgg ccgtgctggt gatcgagcac aacatgcgcg tgatcatggc
      541 gctggcccag cgcgtcgtgg cgcttcagct cggcgaggtg atcgccgacg cgccgcccgc
      601 ggcgatcgtg cgcaacgcgc gcgtgatcga cgcctatctg gggcaggcgt ggcgtccgtg
      661 agcgtggcga gcggccccgg tggcgcgccc atgctcgaag tccgcgcgct cgacgtcgtg
      721 ctcggcgact atcaggtcct ctggggcgcc gagctccgcg tggacgaggg agagatcatc
      781 gccgtgctcg gccccaacgg cgccggcaag tcgacgctga tgaacacggt ctcgggactg
      841 ctggcgccgc gggccggcga ggtcgtgttc cggggtcacc cgatcacggg ccggcccgcc
      901 cacctcgtgg tccgcgaggg gctcggccac gtgctcgagc ggcgccggct ctttccgtac
      961 ctgacggtcc gccagaacgt gctactcggc gcctaccacc cggcggcgcg ggtcggccgc
     1021 gacgcccagt acgatcggat gggcgccctc tttccgatca tcccggcgcg cgccgaccag
     1081 ctcgcccaca cgctctccgg cggcgagcag cagatggtcg cgatcgcccg cgggctcatg
     1141 gcccgccctc ggctgctcat gatcgacgag ccgtttctcg ggctggcccc gcgcgtggtc
     1201 gaccagatcg ccgaggtgat ccaccggatc aacgccgacg gcgtgacggt ggtgttcatc
     1261 gagcagaacg tcgagcgcgc gctgggcctc gcccaccgcg gctacgtgct cgagtccggg
     1321 cgcacggtgc tcgacggccg ctcgggcgac ctcttgcgct cggccgaagt ccggcgcatc
     1381 ttcctgggcg gatagcttca cgaggaggcc tcatggcgga cgacaaggtc gagaccatcg
     1441 tgtcccggag cagcggcacc atcgggcggg cggtgagcga ggcgcggggc gcccggctca
     1501 cgctggactc gtccacccac cccaagcccg acgccctcac gaacagcgag gccttcctgg
     1561 ccggcgtgtc gtcgtgcggc gtgacgttga tcgaggcgca cgcgcagcag accggcgtgc
     1621 cgctcacgcg gatggaggtg acgatcgagg gcgtgcgggc ggccgcggat ccgtcgcggt
     1681 tctcgcgggt caccatgcgc ttcgcgctgg ccggcgtcag ccaggcccag gcggaagacc
     1741 tcgtcaggac ctaccagggg cgctgacccc tctaccgcac ggtcgcgggc gcgaccgcgg
     1801 tgaaggtcga agtcagcacg ctcccggctc gataggcgcg cacgtacggc gaagaacccg
     1861 ggacgtcggg cgaggggcgg gcgtcacggc gcgcggtcgg cgatgagacg ggccttcaag
     1921 atcttgccgg tcgcgctcct cggcaatcgg tccagcacga cgacgcggcg cggatacttg
     1981 aaggcggcca gccgctcgcg gcaccaggcg acgaggtcgt cggggctcac gcgggcgccc
     2041 tcgcgcagcg cgacgaacgc ggccacttcc tcgcc
//
LOCUS       DATIAZ010000236         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_64044, whole genome shotgun sequence.
ACCESSION   DATIAZ010000236 DATIAZ010000000
VERSION     DATIAZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64044"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VLF19_08405"
     CDS             complement(<1..1108)
                     /locus_tag="VLF19_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HSE93314.1"
                     /translation="MDLDRLLIASVEQGASDLHLKPNSPPIFRVHGRLVPRPDLGVIT
                     AAEMDDMARRVLPERLYSQLKDGREIDVAYAVRDHGRFRVNMFLATGLVRSVMRAIPS
                     RKPRFEELGLPVVLEKLAMERRGMVLVTGITGSGKSTTLAAMIDFINRSRNDHIITIE
                     DPIEFAHEDINCVVSQREIGHDSPNFATALRAALREDPDVILVGEMRDPETMSVALHA
                     AETGHLVLSTLHTLNAAETVNRIIGTFPPHQEQQIRDQLAAVIVGVISQRLVPKIGGD
                     GRVPAVEVMIGTALIRDCIREAKRTAEIPAFIAQGTSQYGMQTFDQCLLTLYREGIIS
                     YETAREAATNADDFDLKVRGIFSTGEMTFEGDQKR"
     gene            complement(1118..2137)
                     /gene="recA"
                     /locus_tag="VLF19_08410"
     CDS             complement(1118..2137)
                     /gene="recA"
                     /locus_tag="VLF19_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HSE93315.1"
                     /translation="MAEPKSDRRQALDLAIAQIERQFGKGAVMRLGSGAPLEEIAVVP
                     TGALSLDVALGIGGLPRGRIVEIYGPESSGKTTLALHAVAEAQRLGGIAAFIDAEHAL
                     DPGYARKLGVNIDELLISQPDTGEQALEIAETLVRSNAVDVIVVDSVAALVPRAELDG
                     DMGDSLPGLQARLMSQALRKLTAAIARSGAVMIFINQIREKIGVMFGSPETQPGGRAL
                     KFYASVRIDVRRTEVIKQGTESVGVRTKAKVVKNKLAPPFREGEFDMIYGEGISKSGT
                     VLDAGVEQGVIEKSGTWYTYKSERIGQGRENARKWLQDNPAVLADLEAKIRESLGLRA
                     PAPIK"
BASE COUNT          344 a          862 c          892 g          425 t
ORIGIN      
        1 gccgcttctg atccccttcg aacgtcatct cgcccgtcga gaagatgccg cgcaccttga
       61 ggtcgaagtc gtcggcgttc gtcgccgcct cgcgcgcggt ctcgtaggag atgatgccct
      121 cgcggtagag cgtgagcagg cactggtcga acgtctgcat gccgtactgc gacgtgccct
      181 gcgcgatgaa ggcggggatc tcggccgtcc gcttggcctc gcggatgcag tcccggatga
      241 gcgcggtgcc gatcatcacc tcgaccgcgg gcacccgtcc gtcgccgccg atcttcggca
      301 cgagccgctg ggagatgacg ccgacgatga cggcggcgag ctggtcgcgg atctgctgct
      361 cctgatgagg cgggaacgtg ccgatgatgc ggttcaccgt ctccgccgcg ttcagggtgt
      421 gcagggtcga gagcaccagg tgcccggtct ctgcggcgtg cagcgcgacc gacatggtct
      481 cggggtcgcg catctcgccg acgaggatca cgtccggatc ctcgcggagg gcggcgcgca
      541 gcgcggtggc gaagttcggc gagtcgtggc cgatctcgcg ctggctcacc acgcagttga
      601 tgtcctcgtg ggcaaactcg atggggtcct cgatggtgat gatgtggtcg ttgcgcgaac
      661 ggttgatgaa gtcgatcatc gccgccagcg tggtggactt gccggagccg gtgatgccgg
      721 tgacgagcac catcccacga cgctccatgg cgagcttctc cagcaccacc ggcagcccga
      781 gctcctcgaa cctcggcttg cgggagggaa tggctcgcat gaccgaccgc acgaggccgg
      841 tggcgaggaa catgttgacg cggaaccggc cgtggtcccg cacggcgtag gcgacgtcga
      901 tctcgcgccc gtccttgagc tggctgtaca ggcgctcggg caggacccgg cgcgccatgt
      961 cgtccatctc cgccgcggtg atgaccccca gatcggggcg cggcaccagc cggccgtgga
     1021 cgcggaagat cggcggggaa ttgggcttca gatggagatc cgacgccccc tgctcgacgc
     1081 tggcgatcag cagccggtcg aggtccatgg tcctccccta cttgatcggc gccggcgcgc
     1141 gcaggcccag gctctcgcgg atcttcgcct cgaggtcggc gaggaccgcg ggattgtcct
     1201 ggagccactt cctggcgttc tcgcgcccct gcccgatgcg ttcgctcttg taggtgtacc
     1261 aggtgccgga tttctcgatg acgccctgct cgacccccgc gtcgagcacg gtgccggact
     1321 tggagatgcc ctcgccgtag atcatgtcga actcgccctc gcggaacggg ggggcgagct
     1381 tgttcttgac gaccttggcc ttggtcctca cgccgaccga ctcggtgccc tgcttgatca
     1441 cttccgtgcg ccgcacgtcg atgcgcacgg aggcgtagaa cttcagcgcg cgcccgcccg
     1501 gttgcgtctc cggactcccg aacatgacgc cgatcttctc gcggatctgg ttgatgaaga
     1561 tcatgacggc gccggagcgc gcgatggcgg cggtgagctt gcgcagcgcc tgcgacatga
     1621 ggcgcgcctg caggccgggc agcgagtcgc ccatgtcgcc gtccagctcg gcgcggggca
     1681 ccagcgcggc gacggagtcc accacgatga cgtccacggc gttggaccgc accagcgtct
     1741 cggcgatctc gagcgcctgc tcgccggtgt cgggctgcga gatgagcagc tcgtcgatgt
     1801 tgacgccgag cttcctggcg tacccggggt cgagcgcgtg ctcggcgtcg atgaacgccg
     1861 cgatgccgcc caggcgctgc gcctcggcga cagcgtgcag ggccagcgtg gtcttgccgg
     1921 aggactccgg gccatagatc tcgacgatgc gcccgcgcgg caggccgccg atgccgagcg
     1981 cgacgtcgag gctcaacgcg cccgtcggca cgacggcgat ctcctccagc ggcgccccgg
     2041 agcccagccg catgacggcg cccttgccga actgccgctc gatctgggcg atcgccaggt
     2101 ccagggcctg ccgccggtcg ctcttcggct ccgccatcct tgacctcctg attcgccgtc
     2161 gggacgcctt cgcagggctc cggtaacccg acgtactgcc ctggaccgtg ctgcctcgtg
     2221 tcgcccgtgg gagcgctttc gtcgaactcc ggcgctcgag gttcagcccc tggaccaccc
     2281 cggcgatctc acgctcgcgc gcgtcatctg cgcttcgagc attctaggag tcgcctcggg
     2341 tggcgtcaat gttcgtggaa cgatacggcg cgccccgggg cggaccggcg cgagcggagg
     2401 cgggcagggc gagggtgctg tgggggctac cgggcctgcg tgatgcgacg gctggcggcg
     2461 cctccggcct cggaatgccc ctcgagcatg gcgcgcaggc ggcgcaccag ctcggcgtag
     2521 gag
//
LOCUS       DATIAZ010000237         3497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_327511, whole genome shotgun sequence.
ACCESSION   DATIAZ010000237 DATIAZ010000000
VERSION     DATIAZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327511"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VLF19_08415"
     CDS             complement(<1..698)
                     /locus_tag="VLF19_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HSE93316.1"
                     /translation="MASNGICDRVAIVGMGCTPFGERWDKGAGDLLVDAAKDALASAG
                     IELQHIDAFWLGTLFSGNSGLTLSRPLKIAYKPVTRVENFCATGSEAFRNACYAVASG
                     AYDVAMAIGVEKLKDSGYSGLPGSRAVGDGTQVPLTAPAMFSLLAPAYARKYGVSDEQ
                     LKDVMTRIAWKNHKNGALNPRAQFRTEVPKETIACSPLVAGALGIFDCSGVSDGSAAA
                     IVVRAEDAHRYCDRP"
     gene            complement(884..2926)
                     /locus_tag="VLF19_08420"
     CDS             complement(884..2926)
                     /locus_tag="VLF19_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE93317.1"
                     /translation="MSRRVVRTMCPMNCHPTLCGMLAEVEDGRLVGVRGDPDNPDSRG
                     FLCIRGHASVEIIGNDRRLLHPLVRTARGRDDWRRATWDEALDTIVARVRAVPPEAVA
                     AWSGHGLFANNYGTRIASHLLRRLSNLLGSQWWNATMICWGLGGFGVGLTGALRVSTK
                     EDMSAHSALIVMWGANIASQPNTGRHLAAARRRGARVITIDVRHTEAAARSDDIVIVR
                     PGSDAALALAMMHVIVAEGRHDRDFVARHTVGFEALTAHLVGHSPAWAAPITGVPAER
                     IVALARLYATTRPAMIVLGGSSMHKDASGWTAGRAVACLPALTGNLGVPGGGLGPRHG
                     ASTHGQALGDISADERRRPGPRVPNQMSRITEALLDRRVRVMLLFGTDMLSSYADAGR
                     VAEGLARQDLVVAYDLFMNDTARRHADVVLPATAWLEETGCKSTNTHLYLMPKALEPP
                     GETRPVAWVLRELARRLALEDFWPWVGEDGPSEEGPIDALLDHPSTRHATVAALRAEG
                     GMRALEVSHVAYPDLSFDTPSGKVELYSERAESLGLPPLPVPAPPPASRYPLAFRQGR
                     TLTAFHGFYDHGLALPTLAQADPEPVLWISPDDATARGVADGAPIRLCNERGEMTARA
                     HVTARIPPGVVWMRDGWSGLNRLTSGEPVLTDAAVDVFGFSAGQAAYDAMVEVTPA"
     gene            complement(3248..>3497)
                     /locus_tag="VLF19_08425"
     CDS             complement(3248..>3497)
                     /locus_tag="VLF19_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93318.1"
                     /translation="VKGGPRALTDRQKLFFLTDPLALSQLHFEVWTRPAAHPTWLAAC
                     LQACDGDRSAASDPTARSRPDTDEPPEADGTAPRPACA"
BASE COUNT          476 a         1224 c         1322 g          475 t
ORIGIN      
        1 ggcctgtcac agtagcggtg ggcgtcctcg gcgcgcacga cgatggccgc ggccgagccg
       61 tccgacacgc ccgagcagtc gaagatgccc agggcgccgg ccaccagcgg cgagcacgcg
      121 atcgtctcct tcggcacctc cgtccggaac tgggcgcgcg ggttgagggc cccgtttttg
      181 tggttcttcc aggcgatgcg tgtcatcacg tccttgagct gctcgtcgct gacgccgtat
      241 ttcctggcgt aggcgggcgc gagcaggctg aacatggcgg gcgcggtgag cggcacctgg
      301 gtgccgtcgc cgaccgcgcg ggatccgggc aggcccgagt acccggagtc cttgagcttc
      361 tcgacgccga tggccatggc cacgtcgtag gcgccggagg cgacggcgta gcacgcgttg
      421 cggaaagcct ccgagccggt ggcgcagaag ttctccacgc gcgtgacggg cttgtaggcg
      481 atcttcagag ggcgcgagag cgtgagccca ctgttgcccg agaaaagcgt gccgagccag
      541 aaggcgtcga tgtgctgcag ctcgatgccg gcggaggcca gcgcgtcctt ggcggcgtcg
      601 accaggaggt cgccggcgcc cttgtcccag cgctcgccga acggcgtgca gcccatgccc
      661 acgatcgcca cgcggtcgca gatgccgttg ctcgccatca cgtgcctcct tcgatcgact
      721 ccatcttacg ccggcggccg cccacgtcgg tgaccgtctc gggcccttgc gcgggcgggc
      781 acctcgggtc cgggcgaacg cctcgccccc cgcgccggcc acccgcctca cgcgggccga
      841 ctgccaccct cgcgcgacgc ccgcgcggcc tcgggcacgg cgcctacgcc ggggtgacct
      901 ccaccatcgc gtcgtacgcg gcctggcctg ccgagaagcc gaacacgtcc acagccgcgt
      961 cggtgagaac gggctcgccg gacgtcagcc ggttgaggcc gctccagccg tcgcgcatcc
     1021 agacgacgcc cggcgggatg cgggcggtga cgtgggcgcg cgcggtcatc tcgccgcgct
     1081 cgttacagag gcgaatcggc gcgccatcgg ccacgccgcg cgcggtggcg tcgtcgggcg
     1141 agatccacag cacgggctcg gggtcggcct gggctagggt cggcagcgcc aggccatggt
     1201 cgtagaagcc gtggaacgcc gtcagcgtgc gcccctgcct gaaggcgagc gggtagcgtg
     1261 acgcgggcgg cggtgcggga acgggtagcg ggggcagccc caggctctcg gcccgctcgg
     1321 agtagagctc caccttgccc gacggcgtgt cgaaggagag gtcgggatac gcgacgtgcg
     1381 acacctcgag cgcgcgcatg ccgccctcgg cgcggagcgc ggccacggtg gcgtggcggg
     1441 tcgaggggtg atcgagcagc gcgtcgatcg gaccctcctc gctcggccca tcctcgccga
     1501 cccagggcca gaagtcctcc agggcgagcc gccgggccag ctcgcgcagc acccacgcga
     1561 ccgggcgcgt ctcgccgggc ggctccaggg ccttcggcat caggtagagg tgcgtgttcg
     1621 tgctcttgca gcccgtctcc tcgagccagg ccgtggccgg cagcaccacg tcggcgtggc
     1681 gacgtgcggt gtcgttcatg aagagatcgt aggcgacgac gagatcctgg cgcgcgagcc
     1741 cctcggcgac ccggccggcg tcggcgtacg acgagagcat gtcggtgccg aagaggagca
     1801 tcacgcgcac ccggcggtcg agcagcgcct cggtgatgcg tgacatctgg ttcggcacgc
     1861 ggggacctgg ccgccggcgt tcgtcggcgc tgatgtcgcc gagcgcctgg ccgtgggtgg
     1921 aggcgccgtg gcgcgggccg aggccgccgc cgggcacgcc gagattgccc gtgagcgcgg
     1981 gcaggcaggc gacggcgcga cccgccgtcc atccgctcgc gtccttgtgc atcgaggagc
     2041 cgccgagcac gatcatggcc ggacgcgtcg tcgcgtagag gcgggccagc gcgacgatcc
     2101 gctcggcggg cacgccggtg atcggcgccg cccacgcagg cgagtggccc acgaggtgcg
     2161 cggtgagggc ctcgaagccc accgtgtggc gcgcgacgaa gtcgcggtcg tgccggccct
     2221 cggcgacgat cacgtgcatc atcgccagcg ccagcgcggc gtcgctcccc ggcctgacga
     2281 tcacgatgtc gtcggaccgc gccgccgcct ccgtgtgtcg cacgtcgatc gtgatcaccc
     2341 gggcgccgcg acgccgggcg gccgccaggt ggcgcccggt gttgggctgc gaggcgatgt
     2401 tcgcgcccca catcacgatc agcgccgagt gcgcgctcat gtcctccttg gtgctcacgc
     2461 gcagcgcgcc ggtgaggccg acgccgaagc cgccgaggcc ccagcagatc atggtggcgt
     2521 tccaccactg gctgccgagg aggttcgaca agcgccggag caggtgcgac gcgatccgcg
     2581 taccgtagtt gttggcgaag agcccgtgcc ccgaccacgc ggcgacggcc tccggcggca
     2641 ccgcgcggac gcgcgcgacg atcgtgtcga gggcctcgtc ccacgtggcg cgccgccagt
     2701 cgtcgcggcc acgggcggtg cgcacgagcg ggtgcagcag gcgccggtcg ttgccgatga
     2761 tctcgaccga ggcgtgaccg cggatgcaga ggaagccgcg gctgtcgggg ttatcggggt
     2821 cgccgcgcac gccgaccagc cgaccgtcct cgacctccgc gagcatgccg cagagggtcg
     2881 gatggcagtt catcgggcac atggtgcgga cgacacgtcg gctcatgagc gctcccccct
     2941 ccgcgcgcgc ctcacgtcgc cgcgatgccg ccctgcatgg cgcccgcggg ctgctgcgga
     3001 cgggtggtga acgtcaaccg ccagtcgtcc tcggtccggc tgtcgaggga gagccgccag
     3061 acgtaggcac ggtcgggcgc gaggggcagg tgcggaggtt ggccaccact ggaaagtagc
     3121 agatttccag gggctcgggg tttgcgccat cccgcggccg agtcccggcc acaggcccgc
     3181 gggttgcggg ggcggagagg tgaggaaatg cggcatcgcc cacgcccgtg gcgcaagtgc
     3241 gcggccctca ggcacacgct ggtctcggag ccgtcccatc agcttcagga ggctcatcgg
     3301 tgtctggtcg ggatcgagct gtgggatccg aggcggccga gcggtcgccg tcgcatgcct
     3361 gcaggcacgc ggcgagccag gtgggatgcg cggccggcct ggtccagacc tcgaaatgga
     3421 gctgggagag cgcgagagga tccgtgagga agaacagctt ttgccgatcg gtcagcgccc
     3481 gcgggccgcc cttcacc
//
LOCUS       DATIAZ010000238         3103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1774310, whole genome shotgun sequence.
ACCESSION   DATIAZ010000238 DATIAZ010000000
VERSION     DATIAZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774310"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VLF19_08430"
     CDS             <1..626
                     /locus_tag="VLF19_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSE93319.1"
                     /translation="LKIPDHVGDDHAALTEPLAIAVHDVRRARVQAGDRVLVFGGGPI
                     GTLIALVARQRGARVAVAEVNPFRLEMLGRYGLETVGPEQDVVRWANQWTDGTGVDVA
                     FEVTGNPAAARTVTDVVRVWGTVSVVAIHAEPVPVNLYQMFARELVMHGARLYARDDW
                     EEAIRLIASAAVDVAPLVSRRLPLEALQQGMEEALGGGPVMKVLIVP"
     gene            623..955
                     /locus_tag="VLF19_08435"
     CDS             623..955
                     /locus_tag="VLF19_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93320.1"
                     /translation="MSRPVALITGAGRGIGRATAEAFAAESWAVVAAELRPALGRRVE
                     QALRRAGAVAVFVQTDVSGSFFARPGTAVARLVDRQAPAIRTAARSWRQRSCPSADPR
                     STPGRLPI"
     gene            complement(1062..1634)
                     /locus_tag="VLF19_08440"
     CDS             complement(1062..1634)
                     /locus_tag="VLF19_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSE93321.1"
                     /translation="MTTTPALTIANPSWVADIADGGRVYTSDEDKMRLVVTLARENVI
                     REAGGPFAAAVFEIDTGRLVAAGVNSVVRLRSSALHAEIMALMMAQHGRGAFSLGVAG
                     LPAHGLIVSCEPCAMCLGAILWSGVRHVVHAADGVDARRLGFEEGPVFPETYRYLEDR
                     GIAFTRGVLADDARAVLRLYHERGGPIYNG"
     gene            1716..2867
                     /locus_tag="VLF19_08445"
     CDS             1716..2867
                     /locus_tag="VLF19_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSE93322.1"
                     /translation="MQVTLVTPLLVDPGSGKNWCFVKVETDAGITGWGECYTQADRDQ
                     SIVAHVRQLGRYLARRDPAQIRHFTHVAYHDFAAKRGAMDFWSALSGIEQALWDIAGK
                     RLGAPVYDLLGGPCRDRIRVYANGWYGGAKTPEAYAAKAREVVARGFTALKFDPFPGP
                     WRTHISRRVEDQAVETVGAVREAVGPDVDVLVEVHRRLAPMHAVRVAERLEPFRPFWF
                     EEPVSSRDVEGLAEVRSRIRLPVVTGEELYTKAEFVPVLARRAADILNPDVCNCGGIL
                     ELREIAAMAEPVHVVISPHNYNSTTVGLAATLQVAATIPNFLITEYFLNFEERGNEVA
                     TEPFRVEGGSIRLPTRPGLGIELREDALGHHAYREFPLRNLPVTHDEGP"
BASE COUNT          452 a         1097 c         1131 g          423 t
ORIGIN      
        1 tgctgaagat tcccgaccac gtgggcgacg accacgcggc gctgaccgag ccgctggcca
       61 tcgcggttca cgacgtgcgg cgtgcgcggg tgcaggccgg cgaccgcgtg ctggtcttcg
      121 gcggcgggcc catcggcacg ctgatcgcgc tggtcgcccg gcagcgaggc gcccgggtgg
      181 ccgtggccga ggtcaacccg ttccgcctgg agatgctggg caggtacggg ctggagaccg
      241 tcgggccgga gcaggacgtc gtccgctggg caaaccagtg gacggacggc acgggggtgg
      301 acgtcgcctt cgaggtgacg ggcaacccgg cggcggcccg gacagtgacc gacgtcgtgc
      361 gcgtgtgggg cacggtgagc gtggtggcca tccacgccga gccggtgccg gtgaatctct
      421 atcagatgtt cgcgcgcgag ctggtgatgc acggcgcccg cctctacgcg cgcgacgact
      481 gggaggaggc gatccgcctg atcgccagtg ccgccgtgga cgtggcgccg ctggtgagcc
      541 ggcggctgcc gctcgaggcg ctgcagcagg gtatggaaga ggcactcggc ggcggcccgg
      601 tgatgaaggt gctcatcgtg ccgtgagccg gccggtcgcg ctgatcaccg gcgccggccg
      661 cggcatcggc cgcgcgaccg ccgaggcgtt cgccgccgag agctgggccg tcgtcgccgc
      721 cgagctgcgg ccggcgctcg ggcggcgggt cgagcaggcg ctccggcggg caggcgccgt
      781 ggcggtgttc gtgcagaccg acgtgtcagg ctccttcttc gcacgccccg gcacagccgt
      841 cgcgcgcctg gttgatcgac aagctccggc catccgcacc gcggcccgct cgtggcggca
      901 gcgatcttgc ccaagcgccg accccaggag tacgccgggc aggctaccga tctgagatgg
      961 cccagaagct cggcgacgag cggctgaaag atcccgccgc acagatggag gagatggcgg
     1021 atcaggccag cgggggccgg agggccgcct gacgggcgcg gctacccgtt gtaaatcggg
     1081 cctccgcgct catggtaaag ccggagcacg gcgcgcgcat cgtcagcgag cacccctcgc
     1141 gtgaacgcga tgccgcggtc ttccaggtaa cggtaggtct ccgggaagac gggcccctcc
     1201 tcgaatccca ggcggcgggc gtccacgccg tcggcggcgt ggacgacgtg ccgcacgccg
     1261 ctccagagga tggcgccgag gcacatggca cagggctcgc acgagacgat cagcccgtgc
     1321 gcgggcagcc cggccacgcc gagcgagaac gctccgcgac cgtgctgcgc catcatgagc
     1381 gccatgatct cggcgtgcag ggcggagctc cggagacgga cgacgctgtt gacgccggcg
     1441 gcgacgagcc gaccggtgtc gatctcgaag acggcggccg cgaacggccc gcccgcctcg
     1501 cggatcacgt tctcgcgcgc gagcgtgacc acgaggcgca tcttgtcctc gtccgacgtg
     1561 tagactcgtc caccatccgc gatgtcggcg acccacgagg ggttggcgat cgtcagcgcg
     1621 ggcgtcgtcg tcaactcgcg tctcctctcc ggtgccacgc agagtcgtac gtcgaccgtc
     1681 gagtatagta ggcgggcgct caggaggacg actcgatgca ggtcaccctg gtcacgcctc
     1741 tcctcgtcga tcccggcagc ggcaagaact ggtgcttcgt gaaggtcgag acggacgccg
     1801 gcatcaccgg ctggggcgag tgctacacgc aggccgaccg cgaccagtcg atcgtcgccc
     1861 acgtgcggca gctcggccgc tacctcgcca ggcgcgaccc cgcccagatc cgccacttca
     1921 cccatgtcgc atatcacgac ttcgccgcca agcggggcgc catggacttc tggagcgcgc
     1981 tctccggtat cgagcaggcg ctctgggaca tcgccggcaa gcgcctcggc gcgcccgtct
     2041 acgacctgct cggcgggccg tgccgcgacc gcatccgcgt ctatgccaac ggatggtacg
     2101 gcggcgccaa gacgccggaa gcctacgcgg cgaaggcgcg cgaggtggtc gcgcgaggct
     2161 tcaccgcgct gaagttcgat ccgttcccag gcccctggcg cacgcacatc tcgcgccggg
     2221 tcgaagacca ggccgtcgag acggtgggcg cggtgcgcga ggcggtgggc cccgacgtgg
     2281 acgtcctggt cgaggtgcac cggcggctcg cgccgatgca tgcggtccgc gtcgccgagc
     2341 ggctcgagcc cttccggccg ttctggttcg aggagccggt gtcctcgcgc gacgtcgagg
     2401 gcctggccga ggtgcggagc aggatccggc tgccggtcgt gaccggcgag gagctctaca
     2461 ccaaggccga gttcgtgccg gtgctggcgc gccgggccgc ggacatcctg aaccccgacg
     2521 tctgcaactg cggcggcatc ctcgagctga gggagatcgc cgccatggcc gagcccgtgc
     2581 acgtggtcat ctcgccccac aactacaact cgaccaccgt cggcctggcc gccacgctgc
     2641 aggtcgccgc gacgatcccg aactttctca tcaccgagta cttcctgaac ttcgaggagc
     2701 ggggcaacga ggtcgcgacc gagcccttcc gggtcgaggg cggctccatc cgcctcccca
     2761 cccgccccgg cctcgggatc gagctgagag aagacgcgct cggccaccac gcctaccgtg
     2821 aattccccct gaggaacctc ccggtgaccc acgacgaggg cccgtagagt cggcgccatc
     2881 gggtgccccg gccaggcggc cggtcggggt gaaatgcgca gggttcggag ccggcgaccc
     2941 gcgccgtggc gcgtgacggc ccagcgctac gccaccccga tcgacgcgcg catctcgtgg
     3001 cgctgccccg gagccagggt gacggcatcg tccgcagcgt tcgccgtctc gatacagagc
     3061 atcgagcgcc actcctcggg gccgaagtcg gccatggccc tgg
//
LOCUS       DATIAZ010000239         4808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_983971, whole genome shotgun sequence.
ACCESSION   DATIAZ010000239 DATIAZ010000000
VERSION     DATIAZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983971"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VLF19_08450"
     CDS             complement(<1..1028)
                     /locus_tag="VLF19_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSE93323.1"
                     /translation="MEADRHTVAGPRDAADDREHIRAWHALDVTRVAALLGTDIARGL
                     TGTEARRRLARVGPNRVGEARPRPLWRLALEQFGSLVVLLLLGAAAVAVALGERAEGL
                     AILAALVLNAAIGFGTEWRARVSLSRLRVLVVPAARVRRDARVTTVAAADVVPGDVVV
                     LEAGAQVPADARLARSAGLRVDEAALTGESLPVEKDAAARLDPRAPLAEHPTMVYLGT
                     AVLAGSGIAVVTATGVATQLGRIGQLVALAGDRATPLERQIESLGRWLVGAALAVCAA
                     VAVAGIVRGQPVGLMLETAISLAVSAIPEGLPAVTAVALAAGLWRLARAGALVRRLPA
                     VETLGATTV"
     gene            1238..1447
                     /locus_tag="VLF19_08455"
     CDS             1238..1447
                     /locus_tag="VLF19_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93324.1"
                     /translation="MPLFAVGEEETHQVGDPACFECWEEYPESCSCGGLIHAAGGTEE
                     DEDGNVLLTTRCDRCGRSEEDLGEP"
     gene            complement(1632..2174)
                     /locus_tag="VLF19_08460"
     CDS             complement(1632..2174)
                     /locus_tag="VLF19_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSE93325.1"
                     /translation="MYQRLEEFLGAHQAAYEVVEHEAAITAQEQAAATHAPGRSVAKV
                     LIVKERDGYVMAILPASAELDLDRLKGLVGHGEIRLATVEEIRAVVTDCVPGAIPPFG
                     ALYRLRSFVDRSLLLVPQVTMPAGDPASAIRMRSAEFRRLVDARPGDFAVPEALVTAG
                     GVARPRRARRRGASTSPRRR"
     gene            2613..3782
                     /locus_tag="VLF19_08465"
     CDS             2613..3782
                     /locus_tag="VLF19_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HSE93326.1"
                     /translation="MKVTLSVLKADVGSIGGHTRPSARMLATVRGRVREAVAKGLLID
                     GTVTHTGDDIAITTSHVHGTGDRAIHAFAWDCFIAATGAARERGLYGAGQDLLVDAPS
                     GNIRGAGPAVAEIEFERDPASAERPAEAFLVFAGDKCSPGAYNFPLYTVFCDPMHNGG
                     LLLSPKLHKGFTLTIIDMDHKGDGGDSVIRLDVPERVWDVACLLQNPDRFAVEAIHSR
                     HRPAEQIVAVSATRLHNIAGKYTGKDDPVAIVRTQGIFPAPEEVIEPYMLGHFVTGDA
                     RGSHVMPLMPVAIDTAVAGPYCLPIVSCLAFSMDAGGRFTEEHVDVFGNLAWDATRAK
                     VQAKADEWRRQGFVGPTMASHAELAYTGIVDALGALEREFTIRREGEAALVTARR"
     gene            complement(3786..4229)
                     /locus_tag="VLF19_08470"
     CDS             complement(3786..4229)
                     /locus_tag="VLF19_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSE93327.1"
                     /translation="MSRPAVTIHQDAAARGAWALMRNRGIRHLPVVNAEGGLVGIVTD
                     RDLRQLVFDPAIRARLVASARSDLTVGAVMTRAVITTCPETPILEATRLMHERKIGAL
                     PVVEGDRVTGIFTESDAFAALVQTLGTLAIPRALRGAIATRRNTA"
BASE COUNT          697 a         1771 c         1701 g          639 t
ORIGIN      
        1 acggtcgtcg cgccgagcgt ctcgacggcg ggcaggcggc ggacgagcgc gccggcgcgg
       61 gccagccgcc agagaccggc ggccagcgcc acggccgtca cagcgggcag cccttcggga
      121 atcgccgaca cggccaggct gatcgcggtc tcgagcatga ggccgaccgg ctgcccgcgc
      181 acgatgccgg cgacggcgac ggcggcacag acggcgagcg cggcacccac cagccagcgg
      241 ccaagactct cgatctgccg ctcgagtggc gtcgcgcggt ctccggcgag cgccaccagc
      301 tggccgatcc ggccgagctg cgtcgccacc ccggtcgcgg tcaccacggc gatgccgctg
      361 cccgcgagga ccgccgtgcc gaggtagacc atcgtcgggt gctcggcgag cggcgctcgc
      421 gggtcgaggc gcgcggccgc gtctttctcg accggcaggc tctcccccgt gagcgccgcc
      481 tcgtcgacgc gcaggccggc gctgcgcgcg agccgcgcgt cggccgggac ctgggcgccg
      541 gcctcgagca cgacgacgtc accgggcacc acgtccgccg ccgccaccgt cgtcacgcgc
      601 gcgtcgcgac ggacgcgcgc ggcgggaacg acgagcacgc ggagccgcga cagcgacacc
      661 cgggcccgcc actcggtgcc gaagccgatc gcggcgttca ggaccagcgc ggccaggatc
      721 gccagcccct cggcacgctc cccgagcgcc acggcgacgg cggccgcgcc gaggagcagc
      781 agcacgacga gactgccgaa ctgctcgagc gccaggcgcc agagcggtcg cgggcgcgcc
      841 tcgccgacgc ggttgggtcc cacgcgcgcc aggcgccgtc gcgcctcggt gcccgtgagg
      901 ccgcgggcga tgtcggtccc gaggagtgcc gcgacgcgcg tgacgtcgag cgcgtgccag
      961 gcgcggatgt gctcgcgatc gtccgccgcg tcgcgcggcc ctgcgaccgt gtgccggtca
     1021 gcctccatcg gagcgtctga tgcgcaagcc cggtgccacc cctgcagggc tcgcaccgac
     1081 gcacgcttcg cgcgggcctg cctgaggccc cgacgcctcg ctgcggcgcc cgtgtcccac
     1141 tacaatgatg gccgactcgt cccgacgggg tgggctcgac gaaggtagtg gtcttgccgc
     1201 gcgatcgtga ccgccgtcga gaaagggagg gagccacatg ccactgttcg ctgtcgggga
     1261 agaggaaacc caccaggtcg gcgatcccgc atgcttcgag tgctgggaag agtatccgga
     1321 gtcctgctcc tgcggaggcc tgatccacgc ggccggcggc acggaggagg acgaggacgg
     1381 caacgtcctc ctcacgacgc gctgcgaccg ctgcggacgg tccgaagagg acctcggcga
     1441 accatagcct gcggcggccc ggtcagcgga tcaattcctt gaagcgcggc cacggcagcg
     1501 ccgtccacgt cgcatgacca cctttgccgc gaccgtctcg tccggcgcct cgaacaggtc
     1561 caggtagtca cgggggagtc ttcacggcct cgggtgtcag tcgcgtgagc atcacgtaga
     1621 tcgccatgct gtcatctcct cctgggcgat gtcgatgcgc cacgacggcg cgcgcggcgg
     1681 ggccgcgcca cgccgcccgc cgtgacgagg gcctcgggca ccgcgaaatc gcccggccgc
     1741 gcgtcgacga gacggcggaa ctccgcggag cgcatccgga tcgcggaggc cgggtcgccc
     1801 gcgggcatgg tcacctgcgg caccagcagc aggctccggt cgacgaagct gcggagccgg
     1861 tagagcgcgc cgaacggcgg gatcgcaccg ggcacgcagt ccgtcacgac cgcacggatc
     1921 tcctccaccg tcgccagccg gatctcgccg tgaccgacga gccccttgag ccggtccagg
     1981 tcgagctcgg ctgaagcggg caggatggcc atgacgtacc catcgcgctc cttgacgatg
     2041 agcaccttcg ccacgctccg cccgggcgca tgcgtggcgg ccgcctgttc ctgcgcggtg
     2101 atcgcggctt cgtgctcgac cacctcgtac gcggcctggt gcgcccccag gaactcttcg
     2161 agtcgctggt acatgcctga cccccctctt cgagtcgcgc tcagcgccgc atcgaggccg
     2221 cgacctcggg ctcgatcggc ccccggcgcg ccgcccgacc cagaccgacc tggcggagcg
     2281 ccgaccgcgt caacgaagtt ctcgtagact gctggatctc ccggacgatc tgctcgcgcc
     2341 cgaggcccca cactcgtgaa gcacgcacgg tgcaaagcct tcgccaggcc gccgacgccg
     2401 ggcccccccg gagatcccgt ctcgaaccgc gcggtgtgtg gggcgtggga cgcccacgct
     2461 gtggcgttgc ctggacacgt gacgtgccgc tgccccacgg ccgggccggc accggatgag
     2521 gagcgctggc caagccgccg cttttcctcg caaacgccgg cacatgtatg tggcatcgca
     2581 cttgaacctg cccgtgccga agggggaacg ccatgaaggt aaccctgtcg gtgctcaagg
     2641 cagacgtcgg ctcgatcggc ggccacacgc ggccgtcggc cagaatgctg gcgacggtcc
     2701 gcggccgcgt gagggaggcc gtcgccaagg ggctgctgat cgacgggacc gtcacccaca
     2761 ccggcgacga catcgcgatc acgacgagcc acgtgcatgg caccggcgat cgggcgatcc
     2821 acgccttcgc gtgggactgc ttcatcgccg cgaccggcgc cgctcgcgag cgcgggctct
     2881 acggcgcggg gcaggacctc ctcgtcgatg cgccgtccgg caacatccgg ggtgccggcc
     2941 ccgcggtggc ggagatcgag ttcgagcgcg accctgcgag cgccgagcgc ccggccgagg
     3001 cgttcctggt gttcgccggc gacaagtgca gcccgggcgc ctacaacttc cccctctaca
     3061 cggtgttctg cgacccgatg cacaacggcg ggctgctgct cagccccaag ctccacaagg
     3121 gcttcacgtt gacgatcatc gacatggacc acaagggcga cggcggcgac tcggtgatcc
     3181 gcctcgacgt gccggagcgc gtctgggacg tggcgtgcct gctacagaac cccgaccgct
     3241 tcgccgtcga ggcgatccac tctcgccacc gtccggccga gcagatcgtc gccgtctccg
     3301 cgacccgcct gcacaacatc gccggcaagt acaccggcaa ggacgacccg gtggcgatcg
     3361 tgcgcacgca gggcatcttc ccggcgccgg aggaggtgat cgagccctac atgctcgggc
     3421 acttcgtgac gggggacgct cgaggctccc acgtgatgcc actgatgccg gtcgccatcg
     3481 acaccgcggt ggcgggtccc tactgcctgc cgatcgtctc gtgcctggcg ttctcgatgg
     3541 atgccggggg acgcttcacc gaggagcacg tggacgtgtt cggcaacctc gcctgggacg
     3601 cgacgcgggc aaaggtgcag gccaaggccg acgagtggcg gcgccagggc ttcgtggggc
     3661 cgacgatggc ctcgcacgcg gagctggcgt acacgggcat cgtcgacgcc ttgggcgcgc
     3721 tggagcggga gttcaccatc cggcgggagg gtgaggccgc gctggtcacc gcccggcggt
     3781 gaacctcacg ccgtgttgcg ccgcgtggcg atcgccccgc gcagcgctcg cgggatggcg
     3841 agcgtgccca gcgtctggac cagcgcggcg aaggcgtcgc tctcggtaaa gatccccgtg
     3901 acccggtctc cctcgacaac cggtaacgcc ccgatcttgc gttcgtgcat gagccgggta
     3961 gcctcgagga tcggcgtctc cgggcacgtc gtgatcacgg ctcgcgtcat gacggccccg
     4021 acggtcaagt cgctcctggc ggaggcgacc agacgcgccc ggatggctgg atcgaacacc
     4081 agttgccgca ggtcccggtc ggtgacgatg ccgacgagcc cgccctcggc gttcacgacc
     4141 ggcagatgtc ggatcccgcg gttcctcatc agcgcccatg ccccccgtgc ggccgcatcc
     4201 tggtgaatcg tcacggccgg gcggctcatg cgatcgacca ctcggtctga cgacggcagg
     4261 gcgatgtcct tgtcgactcc gggatcgata cgaacgtgtc tcacttccaa cctcccaacc
     4321 agtgatctgc gcgctcgatc ggccgaacac gagggttgca cgagcagtgc cagaatgccg
     4381 cagaagcatc ccgcccgcga gcgcaggggg ccggtcgaag ggagagccca cgcgagtcat
     4441 cggagacggt gcgcggctgt cccatctggg gcgacgactc ccgggctgag ccgccccact
     4501 atcggaagcg cgtcgcgcgc tcgccgcatt gcacagtcgc ggctccctgg cagtcgcctt
     4561 gcacgcgagc gcgcgcgtga gggcacaccg accgggcgac aggggacggc cgcatcgggt
     4621 gctgtcgatc ctcgcgggcc tcggcgcctc gcccccgtcg gccgaggaaa cgcgcgcccg
     4681 gctcgcgtag ccgccatgat cctcttcagc ctgccccgaa ccgcgtcgct ggcggcggcg
     4741 ctggcgccgc tggcggacgt ctgcgtgggg cgggcggtcg tcggccgctt ctccaacggc
     4801 gagctgca
//
LOCUS       DATIAZ010000240         7437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_984260, whole genome shotgun sequence.
ACCESSION   DATIAZ010000240 DATIAZ010000000
VERSION     DATIAZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984260"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..309)
                     /locus_tag="VLF19_08475"
     CDS             complement(13..309)
                     /locus_tag="VLF19_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93328.1"
                     /translation="MAAASFVIVCPCCAGKLTIDPELRAVIAHEAPPPKRTMADLGTA
                     LGALDRKAAEREERFRQQMQAESQKSKLLDRKFQEGLKKAKDDPDAPRRPFDFE"
     gene            complement(403..1104)
                     /locus_tag="VLF19_08480"
     CDS             complement(403..1104)
                     /locus_tag="VLF19_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93329.1"
                     /translation="MTAVALDVRDIHTAYGLSRVLFGVSIQVAAGECVALLGRNGVGK
                     STTMRSIAGLTPPQAGQVVWKGRDITGWPPYRVARTGIGFVPEDRRIFAELTVWENLD
                     VASRGGNGWTVERVFDLFPTLRELQHRQGGFLSGGEQQMLTIARTLMGNPALLLLDEP
                     SEGLAPLVVDHLGEQIARLKREGLTILLAEQNVAFSLALADRVYVLEKGAIRFSGAAA
                     RLRDDERLRHELLAI"
     gene            complement(1101..1838)
                     /locus_tag="VLF19_08485"
     CDS             complement(1101..1838)
                     /locus_tag="VLF19_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93330.1"
                     /translation="MLEVRDVRKAFDGFQAVAGVSLTVARGEIAAIIGPNGAGKTTFF
                     NLVTGHLTPDAGAVRLNGRDITGLPPHEVCRLGMGRSFQRTNIFPRLTVFQNVQAAFI
                     SHRGRGLDLWTAVDRLYRDEANAVLASIGLLDRAGEVSAFLSHGGRKQLELAIALASE
                     PDLLLLDEPTAGMSATETRETIALVSRVARERGLTLLFTEHDMAVVFSIAQRITVLHQ
                     GRVIAEGAPAEVRADPEVRRVYLGERR"
     gene            complement(1838..2899)
                     /locus_tag="VLF19_08490"
     CDS             complement(1838..2899)
                     /locus_tag="VLF19_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93331.1"
                     /translation="MTVAARVPWGLAAALAALSVPAFGSRFYTFVATDVVILALFAVS
                     LNLLLGYTGLVSFGHAAYFAIGAYTCGILMKKAAAPFALAWPAAGVLAALFALLFGFF
                     CVRLTKIYFAMLTLAFAQIVWAICFKWNDVTGGEQGMPEIPYPDLAWMARLPWLTDLR
                     TSDHFHVLTVVLVGACLWTLRRIVGSPFGRILTTIRENPERAEFIGVDVRRFELYAFV
                     IAGFFAGLSGGLFGIFNRGVFPDFAYWPKSSEVLIMTLLGGMGHFWGPAVGALVLLVL
                     NQQITSYTEYWPFVLGTILVVLLFVFPGGIVGALAQAARVSTWRAPSGPPRGGATRPV
                     GAGAGLDNGGSFTDPGAGR"
     gene            complement(2896..3780)
                     /locus_tag="VLF19_08495"
     CDS             complement(2896..3780)
                     /locus_tag="VLF19_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93332.1"
                     /translation="MPDPSFLFAQALSGLTVAMFLFLIAAGLSLIFGVLRVLNFAHGS
                     FYMLGAYLAYQFVQWAGTGPGRFWWAVLGAAVGIAMLGGLIERLLLRHLYGKDELYQL
                     LFTYALVLILSDVAKVAWGTGQLSVSRPPGLTGALQIFGTYVPYYNLFILLLGPAIAV
                     LVWLVLYRTRAGRFIRAAALDREMLGALGVNVDRLYTGVFVLGSFLAGLGGALVTPVK
                     TIVPGMDVEIIVEAFIVVVIGGLGSFWGTLLGALVYGQVLSFGILIVPRFSIFSVFAL
                     MAVVLILRPWGLLGRPLK"
     gene            complement(3859..5061)
                     /locus_tag="VLF19_08500"
     CDS             complement(3859..5061)
                     /locus_tag="VLF19_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93333.1"
                     /translation="MHRRTVLAVLAVALAGALVVPPTGEAQKPIKVGFPMIMSGPGAL
                     FGEPAAKGAQMFVDEVNARGGVLGRKLELIVRDTKGNADEAVRVSRELILRENVDFLV
                     GTLTSAEGPAVSVVAKENKVVFIAPIPKTDQLTAPDKLHPYVFRVASTTTIEGRTAAE
                     LISKWNVKRIATISPDYAYGQDVTKAFVDYIKKIKPGVEIVDQQWPKLGEADYTPFVN
                     AQMAKKPEAVFSSLWGGHFVTFAKQAKPLGYFEAIKHNFFGAGEAASPETAKAMKDDY
                     PFGIWGNSYDAFYWMDGPPAHREYVRRVSAYLKDEHPSSWAIQGYIGMQALSEAIKKA
                     NSTESDKVARALLGITIDTPHGKLTIREKDHQANRAQIYGKTVKDAKLGFAVMRPATY
                     VDPAKFMD"
     gene            complement(5073..5915)
                     /locus_tag="VLF19_08505"
     CDS             complement(5073..5915)
                     /locus_tag="VLF19_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSE93334.1"
                     /translation="MRYTFEQLGTGFLFTEGPVWHPRGKFLLFSDMPGDHLRRWSATD
                     GVTTFRKPCHQSNGNTYDRQGRLLTCEHATSQVTRTEPDGRLVPIATHFQGKQLNSPN
                     DIVCRSDGGIYFSDPPYGRAKFYGVEREQELDFQGVYRVGPDPRSPELLAGDFDRPNG
                     LCLSLDERRLFVNDTARKHIRVFDVTPTGGLANGRVWAETKGDQPGAPDGMKVDSAGN
                     VYCCGPGGIHVFSPDGSLMEVIEVPEYTANFAWGDDDYRSLFITASKSLYRIRTKVPG
                     NPVF"
     gene            complement(5928..6119)
                     /locus_tag="VLF19_08510"
     CDS             complement(5928..6119)
                     /locus_tag="VLF19_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HSE93335.1"
                     /translation="MPFVNIRLYEGHGKERKDELAQRITSAITEVCKVPKEAVWVVFE
                     DVPPADWYAAGNPGQAVRK"
     gene            6205..7119
                     /locus_tag="VLF19_08515"
     CDS             6205..7119
                     /locus_tag="VLF19_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSE93336.1"
                     /translation="MADLANAFSWSRSRDNTFQDCKRKYFYHYYGAWGGWEATAPEEV
                     RRLYVLKQLASRQQWAGRAVHDAIELALHGMREGRGVPVEPFVTDVIERMRGEWRSSR
                     AGRYRDNPKTAALFEHEYNVELRPEAWQALRENVATCLRNFFRLPLLAEIRKTSPEHW
                     SIEHWSKVFEFEGTQVWVAPDFGYWTPGGRLGLVDWKTGGANGEGAGFQLGCYALYAG
                     EVLGVKPELVDLYEVNLREPQVTSHRWDGERLDQVKEQIRLSIRAMKAYLADPDANTA
                     AITDFERTEELRICRWCNFRAVCRPELS"
     gene            7130..>7437
                     /locus_tag="VLF19_08520"
     CDS             7130..>7437
                     /locus_tag="VLF19_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSE93337.1"
                     /translation="MKPFRDLVSTLDELRALVGEPSDVARKKQVDRLDEHCRDFIARS
                     PFVLMATADAAGRCDVSPKGDPAGFVLVLDDHHLVIPDRPGNRRFDGMRNILANPHIG
                     L"
BASE COUNT         1182 a         2537 c         2643 g         1075 t
ORIGIN      
        1 gggtcggcca tcctactcga agtcgaacgg cctccgcggc gcgtcggggt cgtccttggc
       61 cttcttgagc ccctcctgga acttgcggtc gaggagcttg ctcttctggg actccgcctg
      121 catctgctgc cggaagcgct cctcgcgctc cgcggccttt cgatccagcg cgccgagggc
      181 cgtgccgagg tcggccatcg tgcgcttcgg cggcggcgcc tcgtgcgcga tcacggcccg
      241 cagctccgga tcgatggtga gcttgcccgc gcagcagggg cagacgatga cgaacgacgc
      301 cgccgccatg gcgccctcac gctagcaggt cgccgtcggc cacgcaaccc gcgcggccgg
      361 gcgaaggggg gatcgggggc catgtcgggg cccccgatgc ggtcagatcg ccagcagctc
      421 gtgccgcagg cgctcgtcgt cgcgaagacg cgcggcggcc ccgctgaagc ggatggcgcc
      481 cttctcgagc acgtagacgc ggtcggcgag cgccagggag aacgcgacgt tctgctcggc
      541 gagcaggatg gtcaggccct cgcgcttgag ccgtgcgatc tgctcgccga ggtggtcgac
      601 gacgaggggc gccagcccct cggacggctc gtcgagcagc agcagcgcgg ggttgcccat
      661 gagcgtgcgt gcgatggtga gcatctgctg ctcgccgccc gacaggaagc cgccctggcg
      721 gtgctgcagc tcgcgcagcg tcgggaacag gtcgaacact cgctcgacgg tccagccgtt
      781 gcccccgcgg ctcgccacgt cgaggttctc ccagacggtc agctcggcga agatcctccg
      841 gtcctccggg acgaacccga tgccggtccg ggcgacgcgg tagggcggcc agccggtgat
      901 gtcccgcccc ttccagacga cctgcccggc ctggggcggc gtgagacccg cgatcgagcg
      961 catcgtcgtg gacttgccga cgccgttgcg accgagcagc gcgacgcact ccccggcggc
     1021 gacctggatc gacacgccga agagcacgcg cgacaggccg taggccgtgt ggatgtcccg
     1081 gacgtcgagc gcgacggccg tcaccggcgc tcgcccaggt agacgcggcg cacctcggga
     1141 tccgcccgca cctcggccgg cgccccctcg gcgatgaccc gcccctggtg cagaaccgtg
     1201 atccgctgcg cgatggagaa gacgacggcc atgtcgtgct cggtgaagag cagggtgagg
     1261 ccgcgctcgc gggcgacgcg gctcacgagg gcgatggtct cccgcgtttc cgtcgccgac
     1321 atcccggcgg tcggctcgtc gagcaggagc aggtcgggct cgctggccag cgcgatcgca
     1381 agctcgagct gcttccggcc gccgtgcgag aggaaggcgc tcacctcgcc ggcccggtcc
     1441 agcaggccga tggaggcgag caccgcgttc gcctcgtcgc ggtacagacg gtcgacggcc
     1501 gtccagaggt cgaggccccg gccgcggtgc gagatgaagg cggcctgcac gttctggaac
     1561 acggtgagcc gcgggaagat gttcgtgcgc tggaacgagc ggcccatccc caggcggcac
     1621 acctcgtgtg ggggcaggcc cgtgatgtcc cggccgttga gccgcacggc cccggcgtcc
     1681 ggcgtcaggt ggcccgtgac caggttgaag aaggtcgtct tgcccgcgcc gttcgggccg
     1741 atgatcgcgg cgatctcgcc gcgcgccacc gtgaggctga cgccggcgac ggcctggaac
     1801 ccgtcgaacg ccttgcggac gtcgcgcacc tcgagcatca gcgccccgcg ccggggtccg
     1861 tgaacgaacc gccgttgtcg aggccggccc cggctccgac ggggcgggtc gcgccacctc
     1921 tggggggccc gctcggggcc ctccacgtgc tcacgcgcgc cgcctgggcc agcgcgccca
     1981 cgatgccgcc cgggaacacg aacagcagca cgacgaggat cgtgcccagc acgaagggcc
     2041 agtactccgt gtaggaggtg atctgctggt tgaggaccag cagcacgagc gcgcccaccg
     2101 ccgggcccca gaagtgcccc atgcccccga gcagcgtcat gatgaggacc tccgacgact
     2161 tcggccagta cgcgaagtcg ggaaacacgc cgcggttgaa gatgccgaag aggccgccgc
     2221 tgagcccggc gaagaagccg gcgatgacga aggcgtagag ctcgaaccgg cgcacgtcca
     2281 caccgatgaa ctccgcgcgc tcggggttct cgcggatcgt ggtgaggata cgcccgaacg
     2341 gcgagccgac gatccgccgg agggtccaga ggcaggcgcc caccaggacg accgtgagca
     2401 cgtggaagtg gtcgctggtc cggaggtcgg tgagccacgg cagtcgcgcc atccaggcca
     2461 gatccggata ggggatctcc ggcatgccct gctcgccccc cgtcacgtcg ttccacttga
     2521 agcagatggc ccacacgatc tgggcgaacg ccagcgtgag catcgcgaag tagatcttcg
     2581 tgagccgcac gcagaagaag ccgaagagca gcgcgaagag ggccgccagc acgcccgcgg
     2641 ccggccaggc gagcgcgaac ggcgccgccg ccttcttcat gaggatgccg cacgtgtagg
     2701 cgccgatggc gaagtacgcc gcgtggccga aggacacgag cccggtgtag ccgaggagga
     2761 ggttcaggct caccgcgaag agcgcgagga tgaccacgtc cgtggccacg aacgtgtaga
     2821 accgggagcc gaacgcgggg accgagagcg cggccagggc ggccgcgagg ccccagggga
     2881 cgcgggcggc gacggtcact tgagcggccg ccccagcagg ccccagggcc tcaggatcag
     2941 cacgacggcc atgagggcga acaccgagaa gatcgagaag cgcgggacga tcaggatgcc
     3001 gaaggacagc acctggccgt acacgagcgc gcccaggagc gtgccccaga acgagccgag
     3061 cccgccgatg acgacgacga tgaaggcctc gacgatgatc tcgacgtcca tgcccggcac
     3121 gatcgtcttg accggcgtga ccagcgcgcc ccccagcccg gccaggaacg agccgaggac
     3181 gaacacaccg gtgtagaggc ggtccacgtt gacgccgagc gcgcccagca tctcgcggtc
     3241 gagcgccgcg gcgcggatga agcggccggc ccgcgtgcgg tagagcacga gccagacgag
     3301 gacggcgatg gcggggccca gcagcaggat gaagaggttg tagtagggga cgtacgtccc
     3361 gaagatctgg agggcgccgg tcagacccgg cggtctcgag acggagagct gaccggtgcc
     3421 ccacgcgacc ttcgcgacgt cggagaggat gagcacgagc gcgtaggtga agaggagctg
     3481 gtacagctcg tccttgccgt acaggtgccg gagcagcagg cgctcgatga gcccgcccag
     3541 catcgcgatg ccgaccgcgg cgcccagcac cgcccaccag aaccggccgg gccccgtgcc
     3601 cgcccactgc acgaactggt acgcgagata cgcgcccagc atgtagaagg agccgtgcgc
     3661 gaaattcagc acgcgcagca ccccgaagat cagcgacaac cccgccgcga tcaggaacag
     3721 gaacatcgcg acggtcagcc cgctgagcgc ctgcgcgaac agaaacgagg gatcaggcat
     3781 cgccgctcat gcgtcggtga gaccggtccc caccgctccc acctcgcggt gccgcccccg
     3841 tcccgcggga cacgcctcct agtccatgaa cttcgccggg tcgacgtacg tcgccgggcg
     3901 catgacggcg aagccgagct tcgcgtcctt cacggtcttc ccgtagatct gcgcccggtt
     3961 ggcctggtgg tccttctccc ggatggtcag cttgccgtgc ggcgtgtcga tcgtgatgcc
     4021 gagcaaggcc ctggcgacct tgtccgactc cgtgctgttg gccttcttga tcgcctcgct
     4081 cagtgcctgc atgccgatgt agccctggat ggcccacgac gaggggtgct cgtccttcag
     4141 ataggcgctg acgcgccgga cgtattcccg gtgcgcgggc gggccgtcca tccagtagaa
     4201 ggcgtcgtag gagttgcccc agatgccgaa ggggtagtcg tccttcatcg ccttggcggt
     4261 ctcgggcgag gccgcctcgc ccgcgccgaa gaagttgtgc ttgatggcct cgaagtagcc
     4321 gagcggtttg gcctgcttgg cgaaggtcac gaagtgaccg ccccacagcg aggagaagac
     4381 ggcctcgggc ttcttggcca tctgggcgtt gacgaacggc gtgtagtcgg cctcgccgag
     4441 cttcggccac tgctggtcca cgatctccac gccgggcttg atcttcttga tgtagtccac
     4501 gaacgccttg gtgacgtcct gtccgtacgc gtagtcgggg ctgatcgtgg cgatgcgctt
     4561 gacgttccac ttggagatca gctcggccgc ggtgcggccc tcgatggtcg tggtggacgc
     4621 cacccggaag acgtacgggt gcagcttgtc gggcgccgtg agctgatcgg tcttggggat
     4681 cggggcgatg aagacgacct tgttctcctt ggccacgacg gagacggcgg gtccctcggc
     4741 cgaggtcagc gtgccgacga ggaagtcgac gttctctcgc aggatcagct cgcgcgagac
     4801 gcgcaccgcc tcgtcggcgt tgcccttggt gtcacggacg atcagctcga gcttgcgccc
     4861 gagcacaccg cccctggcgt tgacctcgtc cacgaacatc tgggcgccct tcgccgccgg
     4921 ctcgccgaag agcgcgccgg gaccggacat gatcatcggg aagccgacct tgatgggctt
     4981 ctgcgcctcg ccggtcgggg ggacgaccag cgcgccggcc agggcgacgg cgagcacggc
     5041 caggacggtg cgtcgatgca tgggactctc cttcagaaga cggggttgcc gggaaccttc
     5101 gtccggatgc ggtagaggga cttcgaggcc gtgatgaaga gcgaccggta gtcgtcgtcg
     5161 ccccacgcga agttggcggt gtactcgggg acctcgatca cttccatgag gctgccgtcg
     5221 gggctgaaca cgtggatgcc gcccggcccg cagcagtaca cgttgcccgc ggagtccacc
     5281 ttcatgccgt cgggcgcgcc gggctggtcg cccttcgtct cggcccagac gcgcccgttc
     5341 gcgagtccac cggtgggcgt cacgtcgaag acccggatgt gcttccgcgc ggtgtcgttg
     5401 acgaagagcc ggcgctcgtc gagcgagagg cagaggccgt tgggccggtc gaagtcaccc
     5461 gcgagcagct ccggactcct ggggtcgggc cccacgcggt acacaccctg gaagtccagc
     5521 tcctgctcgc gctccacgcc gtagaacttc gcgcgtccgt acggcgggtc ggagaagtag
     5581 atcccgccgt cgctgcggca gacgatgtcg ttgggactgt tgagctgctt gccctggaaa
     5641 tgcgtggcga tcggcaccag gcgcccgtcg ggctcggtgc gcgtcacctg gctcgtggca
     5701 tgctcgcacg tcagcagccg gccttgacgg tcgtacgtgt tgccgttcga ctggtggcac
     5761 ggcttccgga acgtcgtcac gccgtccgtc gccgaccacc ggcgcaggtg gtctccgggc
     5821 atgtcgctga agagcaggaa cttgccgcgc gggtgccaca ccgggccctc ggtgaagagg
     5881 aacccggtgc ccagctgctc gaacgtgtag cgcaccggct cagcgcgtca cttcctgacg
     5941 gcctgccccg ggttgccggc ggcgtaccag tcggccggag gcacgtcctc gaagacgacc
     6001 cagacggcct ccttcggcac cttgcacacc tcggtgatcg cgctcgtgat gcgctgcgcg
     6061 agctcgtcct tgcgctcctt gccgtggccc tcgtagagac ggatgttcac gaatggcatg
     6121 gcgtgtcctc cgggttagag tgcggagaag gcgcgcgaaa gttaccacag cagcggggtc
     6181 gtctcaagac gtagaatgcg ctcgatggcc gacctcgcca acgccttctc ctggtcgcgg
     6241 agccgcgaca acacgttcca ggactgcaag cggaagtatt tctaccacta ctacggcgcc
     6301 tggggcggct gggaggccac ggcgcccgag gaggtccggc ggctctacgt cctcaagcag
     6361 ctcgcctcgc gccagcagtg ggcgggtcgg gccgtgcacg acgccatcga gctggcgctc
     6421 cacggcatgc gcgaggggcg cggcgtgccc gtcgagccct ttgtgaccga cgtgatcgag
     6481 cggatgcggg gggagtggcg gtcgtccagg gccgggcgct accgcgacaa cccgaagacg
     6541 gcggcgctgt tcgagcacga gtacaacgtc gagctccggc cggaggcctg gcaggccctc
     6601 cgggagaacg tcgccacgtg cctcaggaac ttcttccgcc tgccgctgct cgcggagatc
     6661 cgcaagacct cgcccgagca ctggtcgatc gagcactggt cgaaggtgtt cgagttcgag
     6721 gggacccagg tgtgggtggc gccggacttc ggctactgga caccgggcgg tcgtctcggc
     6781 ctcgtcgact ggaagaccgg cggcgccaac ggcgagggcg cgggcttcca gctcggctgc
     6841 tacgcgctct acgccggcga ggtcctcggc gtgaagcccg agctggtgga cctctacgag
     6901 gtcaacctgc gcgagcccca ggtcacctcg caccggtggg acggcgagcg gctcgatcag
     6961 gtgaaggagc agatccggct ctcgatccgt gcgatgaagg cgtacctcgc cgaccccgac
     7021 gccaacacgg cggcgatcac ggacttcgag cggacggagg agcttcggat ctgccgctgg
     7081 tgcaacttcc gcgcggtgtg ccgcccggag ctgagctgag gagccctcca tgaaaccgtt
     7141 tcgtgacctc gtgagcacac tggacgagct gcgggcgctc gtgggcgagc cgagcgacgt
     7201 ggcgcgcaag aagcaggtcg accggctcga cgagcactgc cgcgacttca tcgcgcggtc
     7261 gcccttcgtg ctgatggcca cggccgacgc cgccggccgc tgcgacgtgt cgcccaaggg
     7321 cgaccctgcc ggcttcgtcc tcgtcctgga cgaccaccac ctcgtcatcc ccgaccggcc
     7381 gggcaatcgc cggttcgacg gcatgcgcaa catcctcgcc aacccgcaca tcgggct
//
LOCUS       DATIAZ010000241         3098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1905488, whole genome shotgun sequence.
ACCESSION   DATIAZ010000241 DATIAZ010000000
VERSION     DATIAZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905488"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /gene="gcvPB"
                     /locus_tag="VLF19_08525"
     CDS             complement(<1..1344)
                     /gene="gcvPB"
                     /locus_tag="VLF19_08525"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HSE93338.1"
                     /translation="MNRSARQGVPQYDRLVFELSSPGRLAYSLPEPDVDVERARAALP
                     AAYLRRESAELPEVSELDVVRHYSRLSQMNYGVDTHFYPLGSCTMKYNPRINEDMARL
                     PGVARLHPLTPEAHAQGALGLVHELAAMLSEIAGMDAVSLQPAAGAQGELAGVLMIRA
                     YHASRGETRTKVLVPDSAHGTNPASTAIAGYEVIEVKSDANGEVDVNDLERHLRPDVA
                     AFMITVPNTLGNFEPRIAEITERCHAAGVQVYMDGANLNAILGVTRPGDLGFDVCHFN
                     LHKTFTTPHGGGGPGAGPLGVKAHLEPFLPVPVVTKQGDHRYRLDWKRPRSIGKLQAF
                     WGNFGMLVRAYTYIRTMGADGLRAVSDNAVLNANYVMKRLERDYDVAAPGPCMHECVL
                     SARRQKKLGVTAMDVAKRLLDLGFYAPSTYFPLIVEEALMIEPTETESKETLDAFC"
     gene            complement(1341..2687)
                     /gene="gcvPA"
                     /locus_tag="VLF19_08530"
     CDS             complement(1341..2687)
                     /gene="gcvPA"
                     /locus_tag="VLF19_08530"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HSE93339.1"
                     /translation="MRYLPNTAQNQRAMLDTIGARSIDDLLTKIPAKARLSRPLALPS
                     ALAETDLVRHLRALAAQNADADSHACFLGAGAYDHYVPSPINHLVLRGEFFTAYTPYQ
                     PEASQGTLRTIYEYQTMMAELTGMDVANASIYDGASSLAEAALMAHAVTGRNEIVLAG
                     SVNPLYRRVAATYCAGPGIALRDVPAPGGVLDLEAARRLVGGKTAALVVQSPNFYGCL
                     EDVAAAAEIAHGAEAMLVVAADPVNLGVLEAPGRLGADIVVGEGQGLGVPLSYGGPYL
                     GVFAAKRELVRRMPGRLVGATVDLDGERGFVLTLQAREQHIRRAKATSNICTNVALCA
                     LMATIYTATLGKQGLRKVGELSMAKAHYASERLAAIPGVALRFDAPFFKEFTLRLPKP
                     PDRVFARLAKQQILPGVPLKTWDRKLADCLLVAVTEKRTREEMDALADALGKAVAR"
     gene            complement(2687..3094)
                     /gene="gcvH"
                     /locus_tag="VLF19_08535"
     CDS             complement(2687..3094)
                     /gene="gcvH"
                     /locus_tag="VLF19_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HSE93340.1"
                     /translation="MTEGLMANIPGDLRYTREHEWAKKDGANVRVGITHFAQEQLGDV
                     VFVELPKAGARVTSHQSFGVVESVKAVSDLFAPLTGEVVEVNAELPKAPELVNRDPYG
                     KGWMIVLKPANPGEWEQLLSAEQYEEHIAQAGH"
BASE COUNT          430 a         1062 c         1122 g          484 t
ORIGIN      
        1 gcagaacgcg tcgagcgtct ccttggactc cgtctcggtg ggctcgatca tgagcgcctc
       61 ctcgacgatc agggggaagt acgtcgaggg ggcgtagaag ccgagatcga gaaggcgttt
      121 cgcgacgtcc atggcggtca caccgagctt cttctgccgg cgggccgaca gaacgcactc
      181 gtgcatgcag ggacccgggg cggcgacgtc gtagtcgcgc tcgagccgct tcatgacgta
      241 gttggcgttg agcacggcgt tgtcgctgac cgcgcggagc ccgtcggcgc ccatggtccg
      301 gatgtaggtg tacgcgcgga cgagcatgcc gaagttgccc cagaaggcct gcagcttgcc
      361 gatcgatctc ggccgcttcc agtccagcct gtagcggtgg tcaccctgct tcgtcaccac
      421 gggcacgggc aggaacggct ccaggtgggc cttgaccccc agcgggcccg cgccgggccc
      481 gccgccgccg tgcggggtcg tgaacgtctt gtggaggttg aagtggcaca cgtcgaagcc
      541 gaggtcgccc ggccgcgtga cgccgaggat cgcgttgagg ttggcgccgt ccatgtacac
      601 ctgcacgccg gcggcgtggc agcgctcggt gatctcggcg atgcgcggct cgaagttgcc
      661 cagcgtgttg ggcaccgtga tcatgaacgc ggccacgtcg ggccgcaggt ggcgctcgag
      721 gtcgttgacg tccacctcgc cgttggcgtc cgacttcacc tcgatcacct cgtagccggc
      781 gatcgcggtg gaggcggggt tggtcccgtg cgcggaatcg ggcacgagca ccttcgtgcg
      841 ggtctcgccg cgcgaggcgt ggtaggcgcg gatcatgagg acgccggcga gctcgccctg
      901 ggcgccggcg gccggctgca gcgacaccgc gtccatcccc gcgatctcgg agagcatcgc
      961 cgcgagctcg tgcaccagcc cgagcgcccc ctgcgcgtgc gcctccggcg tcagcgggtg
     1021 caggcgcgcg acccccggca ggcgcgccat gtcctcgttg atcctggggt tgtacttcat
     1081 cgtgcacgag ccgagagggt agaagtgcgt gtccaccccg tagttcatct ggctgaggcg
     1141 cgagtagtgg cggacgacgt cgagctcaga gacctccggc agctcggcag actctcgccg
     1201 caggtacgcc gccggcagcg ccgcccgcgc acgctcaacg tccacgtccg gctcgggcag
     1261 cgagtaggcc aggcggcccg gtgacgagag ctcgaagacc agccggtcgt actggggcac
     1321 tccctgccgc gccgatcgat tcacctggcc actgccttgc cgagcgcgtc ggccagcgcg
     1381 tccatctcct cgcgggtgcg cttctcggtg accgcgacga gcaggcagtc ggcgagcttg
     1441 cggtcccagg tcttgagcgg cacgcccggg aggatctgct gcttcgccag ccgcgcgaag
     1501 acgcggtcgg gcggcttcgg caggcgcagg gtgaactcct tgaagaaggg ggcgtcgaag
     1561 cgcagcgcca cgccggggat cgccgccagg cgctcggacg cgtagtgcgc cttggccatg
     1621 gacagctcgc cgaccttccg gagcccttgc ttgcccagcg tggccgtgta gatcgtggcc
     1681 atcagcgcgc agagcgcgac gttggtacag atgttcgacg tcgccttcgc gcggcggatg
     1741 tgctgctcgc gcgcctggag ggtcagcacg aacccgcgct cgccgtcgag gtcgaccgtg
     1801 gcgccgacga ggcggccggg catgcggcgg acgagctccc gcttggcggc gaacacgccg
     1861 aggtagggcc cgccgtagga gagcggcacg cccaggccct gcccctcgcc gacgacgatg
     1921 tccgccccga gacgaccggg cgcctccagc acgccgaggt tcacgggatc cgccgcgacg
     1981 acgagcatcg cctccgcgcc gtgggcgatc tcggcggcgg cggcgacgtc ctcgaggcag
     2041 ccgtagaagt tcggcgactg gaccaccagc gccgccgtct tcccgccgac gaggcgccgc
     2101 gcggcctcga ggtccagcac gcccccgggc gccggcacgt cgcgcagcgc gatgcccggt
     2161 cccgcgcagt acgtggccgc cacgcgccgg tagagggggt tcacgctccc ggcgagcacg
     2221 atctcgttgc ggcccgtgac ggcgtgggcc atgagcgccg cctcggccag cgacgaggcg
     2281 ccgtcgtaga tggacgcgtt ggcgacgtcc atcccggtca gctcggccat catcgtctgg
     2341 tactcgtaga tcgtgcggag cgtgccctgg ctcgcctcgg gctgataggg cgtgtaggcg
     2401 gtgaagaact ctccgcgcag gacgaggtga ttgatgggac tcggcacgta atggtcgtag
     2461 gccccggcgc cgaggaagca ggcgtggctg tccgcgtcgg cgttctgcgc ggccagcgcc
     2521 cgcaggtggc ggacgagatc ggtctcggcg agtgcggacg gcagcgccag cggccggctc
     2581 agcctcgcct tggccgggat cttggtcaag agatcgtcga tcgagcgcgc gccgatcgtg
     2641 tccagcatgg cccgctggtt ctgcgcggtg ttcggcaggt agcgcatcaa tggcccgcct
     2701 gggcgatgtg ctcctcgtac tgctccgcgc tgagcagctg ctcccactca ccggggttcg
     2761 ccggcttgag gacgatcatc cagcccttgc cgtagggatc gcgattcacg agctccggcg
     2821 ccttgggcag ctcggcgttg acctcgacca cctcgccggt cagcggcgcg aagaggtcgg
     2881 acacggcctt gaccgactcc acgacgccga acgactggtg ggaggtgacc ctcgcgccgg
     2941 ccttcggcag ctccacgaag acgacgtcgc cgagctgctc ctgcgcgaag tgcgtgatgc
     3001 cgacgcggac gttggcgccg tccttcttgg cccactcgtg ctcgcgcgtg tacctgagat
     3061 cgcccgggat gttcgccatg agtccctctg tcatctta
//
LOCUS       DATIAZ010000242        25415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_722832, whole genome shotgun sequence.
ACCESSION   DATIAZ010000242 DATIAZ010000000
VERSION     DATIAZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 25415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25415
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722832"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..510)
                     /gene="soxY"
                     /locus_tag="VLF19_08540"
     CDS             complement(4..510)
                     /gene="soxY"
                     /locus_tag="VLF19_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HSE93341.1"
                     /translation="MRHGEGMTRRRLIGTLGALGVAGATGAVSLARPAPAAAQPVQPL
                     GAQESVHDALERVFGSRRMKDGTGTVKLDVPLIAENGAVVPVTVEAASPMTPANHVKR
                     VYVVADKNRIPIIARATLFPESGQAFVAANVRLGETGDVRAIVEQSDGTLLQVKREVK
                     VTVGGCGG"
     gene            complement(667..3489)
                     /locus_tag="VLF19_08545"
     CDS             complement(667..3489)
                     /locus_tag="VLF19_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSE93342.1"
                     /translation="MTRLPEEPLHPAARDLPVPTGGLAAYPPAERWDDWEEYDAAAWP
                     RKVKRRYALVPTICFNCEAACGLLAYVDRDTKRIQKFEGNPVHPGSRGRNCAKGPATL
                     NQIADPERIRYPLRRKGPRGGGQWERVTWDEALDDIAGRIRRALVEQRPQEVMYHLGR
                     PGHELVYLQRVFHAWGIDGHNSHTNVCSASARAGYAFWMGMDRPSPDHANARFMLLLS
                     SHLETGHYFNPHAQRIVEGKMRGARICVIDTRLSNTASMADWWLSPWPGSEGALLLAM
                     AQVLIRERRYDRDFVRRWVNWEEFLRSEHPDRPVTFEDFERALDELYAPYTPEFAERE
                     SGVSAATIVEVARAVGQAGPALATHIWRNTAAGNLGGWQVARALQLLVVLMGAVATEG
                     GTAPSPWNKSVPAPPMMPPPGRVWSELLMPREYPLAFFEMSFLLPHFLREGRGKLAMY
                     FTRVYNPVWTNPDGISWMEMLTDEAKVERHACLTPVWSETAWFADYVLPMGHASERHD
                     LMSQETHAARWIGFRQPVLRVLLEREGRRFDTTWQAHEAAGLGQVWEEDELWIELSWR
                     IDPDGALGIRKYFESPYRSGEKLRIEELYRWIFEHSVPGLPETASKEGLTPLAYMRRY
                     GAFLVEEGVYRHHEQALAADDLAGAAVDPVTKLVTRNGQPVGVSIDGTARVGFPTPSR
                     RLEFFSPTLKAWKWPEHAVPAYVRSHVHWSNLDRAAGDMVLIPTFRLPTLIHTRSGNA
                     KWLYEISHTNPIWIHPEDAGRLGVETAGLVKVATAIGHFVDRVWVTEGLRPGIIACSH
                     HLGRWRLAEDAGGERWSTALVDLQRLGPGKWRMRQVHGVEPFASEDPDSGRVWWKEVG
                     VNQNLTFPVQPDPVSGQHCWHQKVRVTRPGPDDRYGDIVVDTDRAHEVYREWLRLARP
                     APGPGNLRRPLWLPRAFKPDAEAYRL"
     gene            complement(3499..5070)
                     /locus_tag="VLF19_08550"
     CDS             complement(3499..5070)
                     /locus_tag="VLF19_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSE93343.1"
                     /translation="MRFGFVIDQRKCIGCHACTVACKEENQVPLGAFRTWVKYVERGT
                     FPHTRRYFSVLRCNHCDEAPCVTICPTVALYRRPDGIVDLDRDRCIGCKSCMQACPYD
                     ALYIDPDTHTAAKCHYCAHRVEAGLAPACVIVCPEQAIVAGDLDDPASPVARLVAREQ
                     VQVRKPEQGTRPKVFYLGADSAALSPELQAPAEGYMWAQRPADEASLVRMVAALRADG
                     RRDGARARAVYDVPHAPRPWGFKVAAYLWAKSVSAGAVLAALPLAGPLAAVIAPALSL
                     AFLALTVALLVLDLKRPDRFFYILLRPNRRSWLVLGAWILVAFGAVVGLALLLGLRGR
                     AVPGLLGLAAAALALAAAGYSALLFGQAEGRDFWQSPLKLPHLVVGAVAAGSAALLVV
                     APGWTGAGASFLESAARITMLDDRGAPALTLAPLLMVALCAHAALIGAELFSRHASHD
                     AALVARLLCRGALAGRLWAGALGAGVAVPLLLLFLAAGTESVALTVAGALLALAGLWI
                     YEDVWIKAGQSIPLS"
     gene            complement(5109..6269)
                     /locus_tag="VLF19_08555"
     CDS             complement(5109..6269)
                     /locus_tag="VLF19_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HSE93344.1"
                     /translation="MAQAFQAQYDILFRRPWTVWGAAVLVAAVNVFLFAFDRPWTASD
                     GARNWGDWLLTGIGALARPDLLPPWLYSGSLLNLGVVAGALAAALLSSEFAVRVPPGA
                     ELIKGAVGGLMLGAGAMLALGCNIGGFFSATSALSLAGLAMMVGLAVGAFLGLRYLLW
                     ETARRPQWSSGGRVFGAARGERSRQPIAGLAVALLLAAAALVYAGVGYATQGIFLLFG
                     VAFGVVFQRSRFCLVRAFREPFLTGDAEHTRAAALAVVVSTVGFTILKFGDLKDRGEW
                     VFPSAGAGALLGGLVFGVGMTLTGGCGAGSLWRAGEGQVKLWAAVAAFALGASVTRLA
                     LVQTGLASKLGAAVFLPAWVGWGGALLLVVAVMAAWATLATWNEVSQKFTAD"
     gene            complement(6367..7152)
                     /locus_tag="VLF19_08560"
     CDS             complement(6367..7152)
                     /locus_tag="VLF19_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSE93345.1"
                     /translation="MTAAFAVTLAALGVAGAFVSGLLGVGGAIVIIPLLLYVPPLVGV
                     GTLDVKAVSAVTMVQVFVSAFAGMLAHRRHQAVNPELAWVGGAAMGTASFVGAMASKW
                     MHDRWLLVVLALMATGAALLMLAPVQALEPVPDAAPRRFSRPRIVVVTVGVGLAAGLV
                     GAGGAFLLVPLLLIVVGVPIRTTIGSSLAITAIASTAGFAGKVLTGQLPFWATLAVLL
                     GAVPGARLGAAVSRRLRPGQLKLVLFLVVLATALRVWWDVLTR"
     gene            complement(7149..7736)
                     /locus_tag="VLF19_08565"
     CDS             complement(7149..7736)
                     /locus_tag="VLF19_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSE93346.1"
                     /translation="MQIVLGVILVLSAAAVATAGPLDVPGAVKAMNCTACHGPAGQSP
                     SSTIPILAGMNAPYFKKAIEDYASGRRPSPEMEPYAKQVRELGMDDIAAYFTAQKQQP
                     TKIKVDAAAVERGRAASMPCTLCHGPRGEGDATKGVPDLRGQPPGYLRNQMILFKQDR
                     RSPGDEKLKALKALMRGIPEESFADLAAYFSSQGR"
     gene            complement(7748..9001)
                     /locus_tag="VLF19_08570"
     CDS             complement(7748..9001)
                     /locus_tag="VLF19_08570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HSE93347.1"
                     /translation="MTLTRRRFLHGSAATLALAAAGCATAMKGEIVPKTGRRVVVIGG
                     GWGGATAAKYVRLADPTVEVVMLEPNREFVSCPFSNLVLSGVRSIESMTFGYGPLRNH
                     GVRIVHETATAIEPDARRVRIGEGWLTYDRLIVSPGVEFQWEQVQGLAENQDKVFHAW
                     KAGPQTVALAKQLQAMPDGGVFVLTIPPAAYRCPPGPYERACQVAWYLKTNKPRAKLI
                     VLDANQNVISKTGLFLAAWKAYPNLEYRASNKVVKVDPSMREVTTDFDDRVKYDVVNV
                     IPPQRAGTIAVQADLVGTDKRWCEVDHVTYESAKHRGVHVIGDSTIGLPVPKSGNVAN
                     AMGKICAVAVTSLLAGKQPPALPPGNTCYSWVNDREAIAVVNTYRIDKGKVVQIEQKL
                     TPVQSAAVAQNAIGWSASIWHDILG"
     gene            9336..10847
                     /locus_tag="VLF19_08575"
     CDS             9336..10847
                     /locus_tag="VLF19_08575"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSE93348.1"
                     /translation="MIRPLHLRMNLAKLGEDNVATFGEYPALVFEGRELTNVDQQRAA
                     SRLAHALRRLGVAPGDRVVVMLPNCPEVLQAYAGILKAGAVVVPVVFLLGPHEVRHIL
                     AHSEARVVITSPDLADRAEGFTGTVILVGGAAPGALAWEELIAREPDRFEAVERDDGD
                     LAVILYTAGTTGQPKGVALSHRNLAANARAAAGLYELDRERWALAVLPLSHSYGLMLA
                     NASAILGTRAVLLRWFNPEAVLETIDRYRVQQMAGVPTMYVYLLNYPEADRFDTSSMR
                     VWGSGAAPLPVEIVEPFERKFGGRIQEGYGLTEASPVVSAHRTSGERRLGSVGQPIPG
                     VEVRIVDDEDRPLPAGQVGEVCVRGENVMLGYYKMPEETARTVRAGWLHTGDMGRLDA
                     DGFLYIVERKKDLVIRGGFNIYPREVEEVLYAHPRVAEAAVVGAPDPVMGEEVVAFVV
                     LKPGQAATAEEVTSFCEERLARFKCPKQVRFVDALPKSPVGKILRKELRGLTR"
     gene            11126..12757
                     /locus_tag="VLF19_08580"
     CDS             11126..12757
                     /locus_tag="VLF19_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93349.1"
                     /translation="MDQSRRDLLTIGGLIAAGATLAPRGVLAQTPKRGGTLTVRMWDP
                     PHFDLHAAGGLSYKLHTLMTYTHSRLVRHKTGPAVQPGTFALEPDLAESWTQPNETTY
                     VFKLRRGVRWHPKPPVNGRELTADDVTYTIHRFLTTKGHGNAHLLKAVDKVEAVDKHT
                     VKFTLKEPYAWFLDTLASPMVVAIVPKEASEKFGDLKKPESFIGTGPWMLDSYRPNQG
                     LTLVRNAQYFIGGLPYIDRVEIVIDEDNASRISSFLAGKYDLGWENPGTINRTDWVQI
                     KDTVKQKRPGIQTTEFPSNVMSHISMRTDQKPFSDVRVRHAISHAIERQGIIDATYEG
                     VGVFNAAVPAALKDWAIPVNQLGEGAKYFKYDPAEAKRLLAAAGYPSGFPGTLCFTTY
                     GSTVLVDQMQLVVKNLKDVGIEAKLDQKEYGAYITSCFYGKFDSMTYGPQTPFLEPDS
                     FLYGQYYPGEPRNQSHINDPVLADMLVRQRRTFDTGKRREVIHEIQRYLASQQYYVTA
                     PSGVYVAVWDRALKNYGPNVGYDYGGRVLTAWLDR"
     gene            12807..13751
                     /locus_tag="VLF19_08585"
     CDS             12807..13751
                     /locus_tag="VLF19_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93350.1"
                     /translation="MLRRYFAKRLLVAVPSLLIATVIVFTLPRLIPGDVVQLMLDEKA
                     YAKDIDELREKLGLNRPIYLQYFEWAGRVLRGDLGESLWTKRPVLEEIGRRLPITLEL
                     ALLASLVAVAISLPIGIVSATRQDTLQDYLARSAAILGLSMPGFWLATLVIVLPAIWW
                     GWRPVSGYVEFSQDPVGHVTQLFLPAVILGIASAAALMRLTRGMLLEVLRQDYVRTAW
                     AKGLHERVIVLKHSLRNAVIPVVTVLGIQIAQIIGGTVIIEQIFGIPGMSRFLFDAIN
                     QRDYPVIQGVNLIVVSFVVLMNLTVDALYAVLDPRIRY"
     gene            13852..14715
                     /locus_tag="VLF19_08590"
     CDS             13852..14715
                     /locus_tag="VLF19_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93351.1"
                     /translation="MAHRQTRRITRGLWWFARKKPLGALGGVIVVGMLLMTVFAERIA
                     PYDYDETIRGARMRPPGAAHWLGTDNLGRDIASRIVYGARVSVTVGFAAIGLGIVLAT
                     VIGVTSGYFGGAYDIVAQRIVDAWMSFPYLVVILSVMAVLGPGLLNVILSLAVIVGAI
                     NSRVIRGATIATAQHVYVEAARAMGASHGRMIVRHILPNVAATIIIVSTIGLGGVILA
                     ESALSFLGFGVPPPYPSWGAMLSGSGRTYMFRAPWMAIWPGVAISLAVFGFNMLGDAV
                     RDVLDPRLRGS"
     gene            complement(14832..15554)
                     /locus_tag="VLF19_08595"
     CDS             complement(14832..15554)
                     /locus_tag="VLF19_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93352.1"
                     /translation="MIVATGLRSESCASGTPALWLVVVARDQRPLHEALVRRFAGDPG
                     TRVVLDRRQGERRQRELLPDLERRRAERRRPAGYHHDLRFQWVAVCREQWRRTRDGDL
                     GTSDNRREGGQAGMSITDVGGETKQQVERWIEESQYLLGRVIPSVFDENQRLRDKAVS
                     AEQDCDRMREEIGALRREINDLHAELNQLRGQHDYLRGEQAAIAEALTRAVHHMTQMT
                     QPINEMVAKLHAGQTAMEGSLQ"
     gene            complement(15645..17720)
                     /gene="fusA"
                     /locus_tag="VLF19_08600"
     CDS             complement(15645..17720)
                     /gene="fusA"
                     /locus_tag="VLF19_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSE93353.1"
                     /translation="MIEVGKIRNVGIVGHGGVGKTSLVEALLYGAGAINRLGKVDDGS
                     TTTDFDPDEIKRKISVNTAVAYCDWKGCRINLVDTPGYGDFIADARAGLRVVEGALVV
                     VDGVAGVQVQTEKVWKFASEYNLPRAIVVNRLDRERADFYRTLESLERRLKGRIVPLQ
                     IPVGEESGFRGFVDLGKMKAVVHEGSKTSETEIPADVAERAKQYREKLVEAAAETDDD
                     LLNKYLEEGGLGDAEMLKALRLGITQGKIVPVLCASATRGIGTAPVLDLLVHEFPSPA
                     DRGEVPGIEVKTKQAGTRAPDPKAPVTALVFKTLSDPHVGKLTLFRVFSGTVRSESQL
                     LNVSRGTRERMSHVSWLMGKTGKTVEALGPGEIGVVAKLKETLTGDTLADEAQAFELP
                     RITFPEAAISFAIQPKTRGDEDKISNALARIAEEDPTVHYHFDPETKQLLVSGVGNLH
                     VEMVVERMKRKYNVDVNLLPPRIPYKETVKGRAEGQGKYKKQTGGRGQYGDTWLRVEP
                     LARGNGFEFVDDIFGGAIPRNFIPSVEKGVRDCMKRGILAGYPIVDLKVTLYDGSYHD
                     VDSSDMAFQIAASMGLQKVFMDAHPILLEPIMNVEVTCPSDNAGDVIGDLNSRRGRIV
                     GMEPAGETAAVRAQAPMAEMLTYEPALRSMTGGRGGYSMEFSHYEEVPAFMADKIIKE
                     AKAEKEKAH"
     gene            complement(17827..19062)
                     /locus_tag="VLF19_08605"
     CDS             complement(17827..19062)
                     /locus_tag="VLF19_08605"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSE93354.1"
                     /translation="MSFVTHVECTVCGRRHDAKRPLTVCEQCGQMLAARYDVDRVRGS
                     VTKEALRVRPPGMYRFRELTPLDDGEEPVTLGEGGTPLLPLPRLAAHLGLRHLWGKDE
                     GQNPTGTFKARGLGMAITRARTLGARGFLIPSAGNAGGAAALYAARAGLPCGVIVPRG
                     TPPAAIAEALIAGGRVFTIEGSIATAGRIVAKVAPRIGWFDLSTLKEPYRLEGKKTMG
                     LELAEQLGWTLPDVLLYPTGGGTGLVGIPKGFDELRAIGWISGRGPRFVSVQAEGCAP
                     VVKAWRDRAEVTTAWERPVTHAAGLRVPAPFAGRQMLRIVRESGGEAIAVSETAIRDA
                     QRLLARLDGVWTAPEAAATIAALIEMKERGAVAADTRIVTVLTGAGIKNDPPPLPPPS
                     DLSGPDDEVVARVTSILAS"
     gene            complement(19093..19848)
                     /locus_tag="VLF19_08610"
     CDS             complement(19093..19848)
                     /locus_tag="VLF19_08610"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93355.1"
                     /translation="MRFPGRVVIVTGAARGIGAVTAEAFAREGARLAALDLDGPGAEA
                     TATRLRESGAETLAFGADAAVPARIREVVEAIVDRWGRIDVLVNNAGGFAAIRATEDI
                     TDEEWEMILRSNLTSAFVCCRAVLPVMKRQRSGRIVNVASVVARGGAVRVTSHYAAAK
                     AGVVGFTRHLALEAGPDGITVNAVAPGTTATERVKALRTPEESRRLAEQTIPLRRLGE
                     PSEIADAILYLASDAASFVTGATLDVNGGQVMA"
     gene            complement(19845..20786)
                     /locus_tag="VLF19_08615"
     CDS             complement(19845..20786)
                     /locus_tag="VLF19_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSE93356.1"
                     /translation="MRLVTYRKGPSRPKIGAATPGAVLDLGALAVDLARERGAVRHGR
                     GGFPTTMLELIAGGAEALGRAREALAHGEGLLERHGVDGLVERRLGLAAAKARLEAPI
                     PRPARNVFCLGRNYKEHAAERGAEAPSHPVYFTKVPESVIAPGGKIVHHAVTKELDYE
                     VELAVVVGTAGRDVPRERALEHVFGYTVVNDVTARDLQKRHGQWFKGKTLDTFCPMGP
                     VLVTADEIPDPQALAITLRVNGETRQASHTSKMIFPVDECISVLSEGFTLLPGDVIAT
                     GTPAGVGAALGEFLRVGDTLEAEVERIGVLQNHVVAP"
     gene            complement(20805..21797)
                     /gene="ispH"
                     /locus_tag="VLF19_08620"
                     /gene_synonym="lytB"
     CDS             complement(20805..21797)
                     /gene="ispH"
                     /locus_tag="VLF19_08620"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSE93357.1"
                     /translation="MSTPIAEIVLASPRGFCAGVERAIDIVELALEVCQPPVYVRKEI
                     VHNGHVVEKLRAKGAIFVDELDDVPDRATAVFSAHGVSPAVREDAARRGLRVIDATCP
                     LVTKVHLEAIRYAREDFSIVLIGHEDHDEVIGTLGEAPDRIFVIDSVSEVEQLRVPNP
                     DKVAYLTQTTLSVDDTRDVIEALREKFPKIVGPSRDDICYATQNRQAAVKTVAGDVDV
                     LLVIGAKNSSNSIRLVEVSRSAGTRAYLINDVRDIEPAWLAGARRVGLTAGASAPEVL
                     VREAVDALRRIGSDVREVHVVDENVRFALPPELEQMARERGITLPSRTVMRQTI"
     gene            complement(21808..22428)
                     /locus_tag="VLF19_08625"
     CDS             complement(21808..22428)
                     /locus_tag="VLF19_08625"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HSE93358.1"
                     /translation="MSRRPGSPTVLAGVLLVVAVLAGPVPGVTYDSSEVWRKGTWVWS
                     LEGAYGNQVNLENFRTFTDLEFVSVGARWSLLPFNIIAPDSVLRSAFEIGLEPLYMHY
                     LNPDDAFYAGLAAVARYHFLRLGRFVPYLEGAAAVGGTNLDINEIDSDASILLIAGIG
                     ASVFITDHHALYAGYRFTHNSNGNTDSPNRGWEAHTGVVGVSFFFK"
     gene            complement(22425..23450)
                     /locus_tag="VLF19_08630"
     CDS             complement(22425..23450)
                     /locus_tag="VLF19_08630"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSE93359.1"
                     /translation="MRAVVFHGPGDLRFEELPWPEPARGEVVVRIDAALTCGTDVKTL
                     RRGHPVMIPRVPTVFGHELAGTITAVGGGVPGLREGDRVVAANSAPCGTCACCRAARP
                     NLCEDLLFVNGAYGEAIALPPRLVAKNVVRLAATLSAARAAFAEPLACALLGIERARL
                     ESGQTVVVLGHGPLGCLLAMVAAARKARVIVVGKRGWRLDRVRGLGIAECVDATASPD
                     LVAELRTMTGGRGPEVAIDATGRPEAWSQAMDAVGRGGAVVFFGGCAPGTTVALDTRR
                     AHYEELTLVGAFHHTPELIRRAVELLEAETVNPDGLLTHRMGLDRVGEALELMSRGEA
                     LKVLVQP"
     gene            complement(23447..24451)
                     /locus_tag="VLF19_08635"
     CDS             complement(23447..24451)
                     /locus_tag="VLF19_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSE93360.1"
                     /translation="MKAAVLIAPGRIVAEDWPAPRIGPGELLLRLRGCGLCGSDIAKI
                     GARGTRTPLVLGHELVGDVLEAGAETRFGAGDRVVAAHHVPCEGCHYCRRGSHSMCPA
                     FKTSHLDPGGFAELVRVPAPNVAHATFRVPPHVSDETASFVEPLACCLRAVRRAAVEP
                     GDSAVVVGLGSIGCLFVQLLERAGAAVVGVDQQRHRVESARALGLPVMDVDEAATAVP
                     RLSGGRGADLVLITGGAAGVLEWAVRTVRDGGAIHYFAGGPGDALPVALDTLYHRELT
                     ITTTYSSSPADLAEAFGLVTKGQVRLDGLYTHRRPLERLDEAVELMRTAQALKVYVTP
                     "
     gene            complement(24448..25335)
                     /gene="hpnD"
                     /locus_tag="VLF19_08640"
     CDS             complement(24448..25335)
                     /gene="hpnD"
                     /locus_tag="VLF19_08640"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HSE93361.1"
                     /translation="MSPADLVSRLTRRSRSNFYYAFLVLPRPRRDALYAVYAFCRTVD
                     DIADLGSERGATPAAMRAELDTWRREVARCYAAGEAPEQPIARQLRAAVQRYPIPRAA
                     LEAIIEGVAMDVDGVTFETAEDLYPYCYRVASAVGLASIEIFGYTDPRAREYAVSLGL
                     ALQLTNILRDVGVDARAGRVYLPQTDLRACGVSVDDLRAARYTPGFVAVMERQAARAR
                     EFYGRARAAFPRVDARSLVAAEIMGRIYHALLGEIEARRFQVFGERVTVPARRKAAIA
                     LGCWAAARLPGARLLRAAL"
BASE COUNT         3717 a         9205 c         8867 g         3626 t
ORIGIN      
        1 cccctaaccg ccgcagccgc cgacggtgac cttgacctcg cgcttcacct ggagcagggt
       61 gccgtcgctc tgctccacga tcgcgcgcac gtcgccggtc tcgcccagcc tgacgttcgc
      121 ggccacgaag gcctgaccgg actccggaaa gagcgtggcc cgcgcgatga tcgggatgcg
      181 gttcttgtcg gcgacgacgt agacgcgctt gacgtggttc gccggcgtca tcggcgaggc
      241 cgcctcgacc gtcaccggca cgaccgcgcc gttctcggcg atcagcggca cgtcgagctt
      301 gacggtcccg gtaccgtcct tcatccgccg gctgccgaag acgcgttcga gcgcatcgtg
      361 tacgctctcc tgggccccga gcggctgcac cggctgcgct gcagccggcg ccggccgggc
      421 cagcgacacg gccccggtcg cgcccgccac ccccagcgca ccgagggtac cgatcaagcg
      481 ccgtcgcgtc atcccctcgc cgtgcctcat gccaccctcg caagccgtcc cgagaacggc
      541 aagcatcagt atacccactc tcgaccttca agtacgggaa ccagtcgtgc tcgggtttgc
      601 atcggagcag cgcaaggcgc tccggaactc agtcgccgca cggcacgcga cccggggtcg
      661 gggaggtcag agccggtagg cctcggcgtc gggcttgaag gcccgcggca gccagagcgg
      721 ccggcggagg ttgccgggac cgggcgccgg ccgcgcgagg cgcagccact cgcggtacac
      781 ctcgtgcgcc cggtcggtgt ccacgacgat gtcgccatag cggtcgtcgg gacctggccg
      841 ggtgacgcgt accttctggt gccagcagtg ctggccactg acgggatcgg gctgcaccgg
      901 gaaggtcagg ttctggttga cgccgacctc cttccaccag acgcgcccgg agtccgggtc
      961 ctcgctcgcg aagggctcga cgccgtgcac ctgccgcatc cgccacttgc cgggccccag
     1021 acgctgcagg tccaccagcg ccgtagacca gcgctcgccg ccggcgtcct cggcgagccg
     1081 ccagcggccg aggtggtgcg aacacgcgat gatgcccggc cggagcccct cggtcaccca
     1141 cacgcggtcc acgaagtggc cgatcgccgt ggccaccttc accaggcccg ccgtctcgac
     1201 gccgaggcgt cccgcgtcct cggggtgaat ccagatcggg ttcgtgtggg agatctcgta
     1261 gagccacttc gcattgcccg agcgcgtgtg gatcagcgtc ggcagccgga acgtcggtat
     1321 cagcaccatg tcgccggcgg cgcggtcgag gttcgaccag tggacgtggc tccggacgta
     1381 cgcgggcacc gcgtgctccg gccacttcca cgccttgagc gtcggcgaga agaactccag
     1441 gcggcgcgag ggcgtgggga accccacgcg cgcggtcccg tcgatgctga cgccgaccgg
     1501 ctggccattc cgcgtgacga gcttcgtgac gggatcgacg gcggcgccgg cgaggtcgtc
     1561 ggcggccagc gcctgctcgt gatggcggta cacgccctcc tcgaccagga acgcgccgta
     1621 ccggcgcatg tacgcgagcg gcgtgagccc ctccttcgag gccgtctccg gcaggcccgg
     1681 gaccgagtgc tcgaagatcc accggtacag ctcctcgatc cgcagcttct cgccggaccg
     1741 gtacggcgac tcgaagtact tcctgatgcc gagcgcgccg tcggggtcga tgcgccacga
     1801 cagctcgatc cagagctcgt cctcctccca cacctgcccg aggcccgccg cctcgtgggc
     1861 ctgccacgtg gtgtcgaagc gacggccctc ccgctcgagc agcacgcgca gcaccggctg
     1921 gcgaaagccg atccaccgcg ccgcgtgcgt ctcctgcgac atcagatcgt ggcgctcgga
     1981 ggcgtggccc atcggcaaca cgtagtcggc gaaccatgcg gtctcgctcc acaccggcgt
     2041 gaggcaggcg tggcgctcga ccttggcctc gtcggtgagc atctccatcc aggagatccc
     2101 gtcggggttg gtccacacgg ggttgtagac gcgcgtgaag tacatggcga gcttgccccg
     2161 cccctctctc aagaagtgcg gcagcaggaa cgacatctcg aagaacgcca gcgggtactc
     2221 ccgcggcatc aggagctcgc tccacaccct gcccggcggg ggcatcatcg gcggcgcggg
     2281 gacggacttg ttccacgggc tcggcgccgt gccaccctcg gtggccacgg cgcccatcag
     2341 cacgaccagg agctggagcg cgcgcgccac ctgccagccc cccaggttgc cggccgcggt
     2401 gttgcgccag atgtgcgtgg ccagggccgg gcccgcctga ccgacggccc gggcgacctc
     2461 gacgatcgtc gccgcgctca cgccgctctc ccgctcggcg aactccggcg tgtacggagc
     2521 gtacagctcg tcgagcgcac gctcgaagtc ctcgaaggtc accggccggt ccgggtgctc
     2581 ggacctcagg aactcctccc agttgaccca gcgccgcacg aagtcgcggt cgtaacgccg
     2641 ctcacggatc agcacctgcg ccatcgccag cagcagcgcg ccctccgacc ccggccacgg
     2701 cgagagccac cagtcggcca tcgacgccgt gttgctgagc cgcgtgtcga tcacgcagat
     2761 cctggcgccc cgcatcttgc cctcgacgat ccgctgggcg tgcggattga agtagtgccc
     2821 ggtctcgagg tgcgaggaca gcagcagcat gaaccgcgcg ttggcgtgat cgggcgaggg
     2881 ccggtccatg cccatccaga atgcgtagcc ggcccgggcg ctcgccgagc agacattggt
     2941 gtggctgttg tggccgtcga tgccccaggc gtggaagacg cgctgcaggt agacgagctc
     3001 gtgccccgga cggccgaggt ggtacatcac ctcctgtggg cgctgctcga cgagcgcccg
     3061 gcggatgcgc ccggcgatgt cgtcgagcgc ctcgtcccag gtgacccgtt cccactgccc
     3121 gccgccccgc ggccccttgc gccgcagcgg atagcggatg cgctcggggt cggcgatctg
     3181 gttcagcgtg gccgggccct tcgcgcagtt gcgcccacgg ctgccggggt gaacggggtt
     3241 gccctcgaac ttctggatgc gcttcgtgtc gcggtccacg taggcgagca gcccacaggc
     3301 ggcctcgcag ttgaagcaga tggtcggcac gagcgcgtag cgccgcttca ccttccgggg
     3361 ccacgccgcc gcgtcgtact cttcccagtc gtcccagcgc tcggcgggcg gataggcggc
     3421 gagcccgccg gtgggcaccg gcaggtcgcg ggcggcagga tgcagcggct cctctggcag
     3481 cctcgtcatt cggtcccctc aggacagcgg aatcgactgc cctgccttga tccagacgtc
     3541 ctcgtagatc cacaggccgg cgagggccag cagcgccccg gcgacggtca gcgcgaccga
     3601 ctccgttccc gcggcgagga agagcagcag cagcggcacc gccacgccgg cgccgagcgc
     3661 gccggcccag agacgcccgg ccagcgcgcc ccggcacagc agacgggcga ccagcgccgc
     3721 atcgtggctg gcgtggcggc tgaacagctc ggcgccgatc agcgcggcgt gggcgcagag
     3781 ggccaccatg agcagcggcg ccagcgtcag tgccggcgcg ccccggtcgt cgagcatcgt
     3841 gatgcgcgct gcggactcca ggaacgaggc gccggcgccc gtccagccgg gcgccacgac
     3901 cagcagcgcc gcgctgcccg cggcgacggc gccgacgacc aggtgcggca gcttcagcgg
     3961 gctctgccag aagtcgcgcc cctcggcctg gccgaagagg agcgcgctgt acccggcggc
     4021 ggccagcgcg agcgccgcgg ccgcgagccc cagcagcccg ggcaccgccc ggcccctcag
     4081 cccgaggagc agcgccagac cgacgacggc gccgaaggcc acgaggatcc acgcgcccaa
     4141 gacgagccac gagcgccggt tcggcctgag caggatgtag aagaagcggt cgggccgctt
     4201 caggtcgagg accagcagcg cgaccgtgag cgcgaggaac gcgaggctca gcgccggggc
     4261 gatcaccgcg gccagcgggc ccgcgagcgg cagcgcggcg agcacggcgc ccgccgacac
     4321 cgacttcgcc cacagatatg cggcgacctt gaatccccac ggtcgaggcg cgtgcggcac
     4381 gtcgtacacg gcgcgcgcgc gcgcgccgtc gcgccggccg tcggctcgaa gggcggccac
     4441 catgcgcacc agcgacgcct cgtccgcggg gcgctgggcc cacatgtagc cctcggccgg
     4501 cgcctgcagc tccgggctca gcgcggccga gtcggcgccg aggtagaaca ccttgggtcg
     4561 cgtcccctgc tcgggcttgc gcacctgcac ctgctctcgc gcgaccagac gcgccacggg
     4621 gctcgccggg tcgtcgaggt cgccggcgac gatggcctgc tcgggacaga cgatcacaca
     4681 cgccggggcg aggccggcct ccacgcggtg cgcgcagtag tggcacttgg ccgcggtgtg
     4741 cgtgtcggga tcgatgtaga gggcgtcgta ggggcaggcc tgcatgcagg acttgcagcc
     4801 gatgcagcgg tcgcggtcga ggtccacgat gccgtcgggc cggcggtaga gggcgacggt
     4861 ggggcagatg gtcacgcacg gtgcctcgtc gcagtgattg catcgcagca ccgagaagta
     4921 ccgccgcgtg tggggaaacg tgccgcgctc gacgtacttc acccacgtcc ggaacgcgcc
     4981 gagcgggacc tggttctcct ccttgcaggc cacggtgcac gcatgacagc cgatgcactt
     5041 gcgctggtcg atcacgaagc cgaaacgcac ggagccggac tcctccctga cgaccaagcg
     5101 cccgtttact aatccgccgt gaacttctgg ctgacctcgt tccaggtggc cagcgtggcc
     5161 cacgccgcca tgaccgcgac gacgaggagg agagcgccgc cccagccgac ccacgccggc
     5221 aggaacacgg cagcgccgag cttgctcgcc agccccgtct gcacgagcgc gaggcgagtc
     5281 accgatgcgc cgagcgcgaa ggcggccacc gccgcccaga gcttcacctg cccctcgccg
     5341 gctcgccaga gggagccggc gccgcagccc cccgtcagcg tcatgccgac cccgaagacg
     5401 aggccgccga ggagcgcccc cgcgccggcg ctcggaaaga cccactcgcc ccgatccttg
     5461 aggtcgccga acttgaggat cgtgaagccg acggtgctga cgaccacggc gagcgcggcc
     5521 gcgcgggtgt gctccgcgtc gccggtgagg aacggctcgc ggaaggcgcg caccaggcag
     5581 aaccgcgacc gctggaagac cacgccgaag gcgacgccga agagcaggaa gattccctgc
     5641 gtggcgtagc cgacgcccgc gtagacgagg gccgcagccg ccaggagcag cgcgacggcc
     5701 aggccggcga tcggctggcg gctccgctcg ccgcgcgccg cgccgaagac gcggccgccc
     5761 gacgaccact gcggccgccg cgcggtctcc cagagcaagt agcggagccc gaggaacgcc
     5821 ccgaccgcga gcccgaccat catcgccagc ccggcgagcg acagtgcgct cgtcgccgag
     5881 aagaagccgc cgatgttgca gcccagcgcc agcatcgcgc cggccccgag catgagcccg
     5941 ccgacggcgc ccttgatcag ctcggcgccc ggcggcacgc ggacggcgaa ctcgctcgag
     6001 agcagcgccg cggcgagcgc gccggcgacg acgccgaggt tcagcagcga ccccgagtag
     6061 agccagggcg gcagcaggtc gggtcgcgcg agcgcgccga tgcccgtgag cagccagtca
     6121 ccccagttgc gcgcaccgtc ggacgccgtc cacggccggt cgaaggcgaa caggaacacg
     6181 ttcaccgcgg ccacgaggac cgccgcgccc cagacggtcc acgggcggcg aaagaggatg
     6241 tcgtactgcg cctggaatgc ttgcgccatc gcgatgccgg caccttaacg cgcagcccgc
     6301 ccggcggcaa gtcgtcccgc cgccccggtc ggtcggaccg gcacacgacc cgccgcgcgc
     6361 gcgccgtcac cgcgtgagca cgtcccacca cacgcgcagc gcggttgcga ggacgacgag
     6421 gaagaggacg agcttgagct gcccggggcg cagccggcgg ctcacggcag cgccgagccg
     6481 tgcgccgggc accgcgccga gcaggacggc gagggtcgcc cagaacggca actgtccggt
     6541 cagaaccttg ccggcgaagc ctgccgtcga ggcgatcgcc gtgatggcga gcgaggagcc
     6601 gatcgtcgtc cggatgggaa cgccaaccac gatgagcagc agcggcacga gcagaaacgc
     6661 gccgcccgcg cccacgagtc ctgccgccaa tcccactccg accgtgacga cgacgatccg
     6721 cgggcggctg aagcgccgag gcgccgcgtc cggcaccggc tcgagcgcct ggaccggcgc
     6781 gagcatgagc agcgccgcgc cggtggccat cagcgcgagc acgaccagca gccagcggtc
     6841 gtgcatccac ttcgaggcca tcgcgccgac gaaggaggcc gtgcccatcg cggcgccccc
     6901 cacccaggcg agctcggggt tcaccgcctg atggcgccgg tgggcgagca ttccggcgaa
     6961 ggcggacacg aacacctgca ccatggtcac tgccgacacg gccttgacat cgagcgtgcc
     7021 cacaccgacg agggggggca cgtagagcag gagcgggatt atgacgatcg cgccgccgac
     7081 tccgagcagg cccgagacga acgcaccggc gacgccgagc gccgccagcg tgacggcgaa
     7141 ggccgccgtc acctgccctg gctgctgaag tacgccgcca ggtcggcgaa gctctcctcg
     7201 gggatgccgc gcatgagcgc cttgagcgcc ttgagcttct cgtcgcctgg actccgtcgg
     7261 tcctgcttga acaggatcat ctggttgcgc aggtagcccg gcggctggcc ccgcaggtcg
     7321 ggtaccccct tggtggcgtc gccctcgcct cgcggcccgt ggcacagggt gcacggcatg
     7381 gaggccgcgc gcccgcgctc gacggcggcg gcatccacct tgatcttggt cggctgctgc
     7441 ttctgcgccg tgaagtacgc ggcgatgtcg tccatgccaa gctctcgcac ctgcttggcg
     7501 tagggctcca tctccggcga cgggcgccgg ccggacgcgt agtcctcgat cgccttcttg
     7561 aagtagggcg cgttcatgcc ggccaggatc ggtatggtcg acgacgggct ctggcccgcc
     7621 gggccgtggc aggcggtgca gttcatcgcc ttcacggcgc cggggacgtc gagcggcccc
     7681 gcggtggcga cggccgcggc cgagaggacg aggatcacgc cgagcacgat ctgcatgggt
     7741 cgccctccta gccgaggatg tcgtgccaga tgctcgccga ccagccgatg gcgttctggg
     7801 ccacggccgc gctctggacc ggcgtcagct tctgctcgat ctgcaccacc ttgcccttgt
     7861 cgatccggta ggtgttgacg acggcgatgg cctcgcggtc gttgacccac gagtagcagg
     7921 tgttgcccgg tggcagggcg ggcggctgct tgccggcgag caggctcgtc acggccaccg
     7981 cgcagatctt gcccatcgcg ttggcgacgt tgccggactt cggcacgggc aggccgatgg
     8041 tggagtcgcc gatcacatgg acgcccctgt gcttcgcgga ctcgtaggtg acgtggtcga
     8101 cctcgcacca ccgcttgtcc gtcccgacga ggtcggcctg cacggcgatc gtgcccgcgc
     8161 gttgcggcgg gatcacgttg acgacgtcgt acttcacgcg gtcgtcgaag tcggtcgtca
     8221 cctcgcgcat ggacggatcg accttcacga ccttgttgga ggcccggtac tcgaggttcg
     8281 ggtacgcctt ccacgcggcg aggaaaagcc ccgtcttcga gatcacgttc tggttggcgt
     8341 cgagcacgat cagcttggcg cggggcttgt tcgtcttgag gtaccacgcg acctggcacg
     8401 cccgctcgta cggaccgggc gggcagcgat aagccgcggg cgggatcgtg agcacgaaca
     8461 cgccaccgtc gggcatcgcc tgcagttgct tcgcgagcgc gaccgtctga gggccggcct
     8521 tccacgcgtg gaagaccttg tcctggttct ccgcgaggcc ctggacctgc tcccactgga
     8581 actcgacgcc gggggacacg atcagccggt cgtaggtgag ccacccctcg ccgatgcgca
     8641 cgcgcctggc gtcgggctcg atggccgtgg cggtctcgtg cacgatgcgc acgccgtggt
     8701 tgcgcagggg gccgtagccg aaggtcatgc tctcgatcga ccgcacgccg ctcaggacga
     8761 ggttactgaa ggggcaggag acgaactcgc ggttcggctc cagcatcacc acctcgaccg
     8821 tcgggtccgc cagccggacg tacttcgccg cggtcgcccc tccccagccg ccgccgatca
     8881 ccacgacccg ccggccggtc ttcggcacga tctcgccctt catggccgtc gcgcagccgg
     8941 cggcggccag cgcgagagtc gcggccgagc cgtgcaagaa gcgtcgtcgc gtgagcgtca
     9001 tggcgtcccc tcctggccga ttggcggggg gattctagca cttcgccccc ttgacggcgg
     9061 aagcctccgg atcatgatcg gccgcccgcc ctggtcatcg ctcgcccgag gtggccgccg
     9121 gggccgaagg tttgtcccac cgacgccagc gacggcgagc cacacgcgaa ccatccgacc
     9181 cggccgggaa cccagcggac cgggccgcac catgatggac ctctgagtgc gggggctcgt
     9241 cctgtccgcc acaggcacct ccctgcagcg gaccgggagg ccccgccagc gcgcaccgac
     9301 acctcgctcc ctttgacggc ggaagtctcc ggggtatgat ccggccactc cacctgcgca
     9361 tgaacctggc gaagctcggc gaggacaacg tcgcgacgtt cggcgagtac ccggcactcg
     9421 tcttcgaggg gcgcgagctc accaacgtcg accagcagcg tgcggccagc cggctcgccc
     9481 acgcgctgcg caggctgggc gtcgcccccg gcgaccgcgt cgtcgtcatg ctcccgaatt
     9541 gccccgaggt gctgcaggcg tacgcgggaa tcctcaaggc cggagccgtc gtcgttcccg
     9601 tcgtgttcct gctgggcccg cacgaggtgc gtcacatcct ggcgcattcg gaggcgcgcg
     9661 tcgtcatcac ctcgcccgat ctggccgacc gggccgaggg cttcacgggg acggtcatcc
     9721 tcgtaggggg cgccgcgccc ggcgccctcg cgtgggagga gctgatcgcg cgggagcccg
     9781 accggttcga ggccgtcgag cgggacgacg gtgacctcgc cgtcatcctg tacaccgcgg
     9841 gcacgacggg gcagcccaag ggggtcgcgc tctcgcaccg aaacctcgcc gccaacgccc
     9901 gggcggcggc gggcctctac gagctggacc gcgagcgctg ggcgctcgcc gtgctgccgc
     9961 tctcgcactc ctacgggctc atgctcgcca acgcgtcggc catcctcggc acccgcgcgg
    10021 tgctgctgcg gtggttcaac cccgaggcgg tcctcgagac catcgaccgc taccgcgtgc
    10081 agcagatggc gggcgtgccg acgatgtacg tctacctgct gaactacccg gaagccgacc
    10141 gcttcgatac ctcctcgatg cgcgtctggg gctccggcgc cgcgccgctg cccgtcgaga
    10201 tcgtggagcc gttcgagagg aagttcggcg ggcggatcca ggagggctac gggctcaccg
    10261 aggcgtcgcc cgtggtctcg gctcaccgca cgtccggcga gcggcgtctc ggctccgtgg
    10321 gccagcccat ccccggcgtg gaggtgcgga tcgtcgacga cgaggaccgc ccgctgcccg
    10381 cgggacaggt cggcgaggtc tgcgtgcgcg gcgagaacgt gatgctcggc tactacaaga
    10441 tgccggaaga gacggcgagg acggtccggg ccggctggct ccacaccggc gacatgggcc
    10501 ggctcgacgc cgacggcttc ctctacatcg tcgagcgcaa gaaggacctc gtcatccgcg
    10561 gtggcttcaa catctacccg cgcgaggtcg aggaggtcct gtacgcccat cccagggtgg
    10621 ccgaggccgc cgtggtcggc gccccggacc cggtcatggg cgaggaggtc gtggccttcg
    10681 tcgtgctcaa gcccggccag gcggcgacgg ccgaggaggt gacctcgttc tgcgaggagc
    10741 gcctggcccg gttcaagtgc cccaagcagg tgcggttcgt cgacgcgctg cccaagagcc
    10801 cggttggcaa gatcctccgc aaggagctgc gcggattgac gcggtgagac aacgccgtcg
    10861 cagttctccc ttgacggcgc gggtccctgc tcttactgtg cgcaccgctt ccggttcgcg
    10921 aacatcccga cggccagacg aaatgggccg ctcgtataac gctgcacacg ctgacgaagg
    10981 gcctcgcctg ctcgagacgc tgggcgccgc cgggagcgat ctcgctcacc gacgccgcgg
    11041 ctcgcggagt cacggaccgc cgtgttccgc ctgctcaaga cgctccagga gcgcgggtag
    11101 ctcggtggac ccaaggagga tctcgatgga ccagagccga cgtgacctgt tgacgatcgg
    11161 tggcctcatc gccgccggcg cgacgctcgc gccgcggggg gtgctggcgc agaccccgaa
    11221 gcgcggcggc acgctgacag tccgaatgtg ggatccgcct cacttcgatc tgcacgccgc
    11281 cggcggactc tcgtacaagc tgcacacgct catgacgtac acgcacagcc ggctcgtgcg
    11341 ccacaagacg gggcccgcgg tgcagcccgg cacgttcgct ctggagcccg acctcgccga
    11401 gtcgtggacc cagcccaacg agaccaccta cgtcttcaag ctccggcgcg gcgtgcgctg
    11461 gcacccgaag ccccccgtga acggccgcga gctgaccgcc gatgacgtga cctataccat
    11521 ccaccggttc ctgacgacca agggccacgg caacgcccac ctgctgaagg cggtggacaa
    11581 ggtcgaggcc gtcgacaagc acaccgtgaa gttcacgctg aaggagccgt acgcctggtt
    11641 tctcgacacg ctggcgagcc cgatggtcgt ggccatcgtg ccgaaggagg cctcggagaa
    11701 gttcggcgac ttgaagaagc cggagtcctt catcggcacc ggcccctgga tgctcgacag
    11761 ctatcgcccc aaccaggggc tgacgctggt ccgcaacgcc cagtacttca tcggcggcct
    11821 gccctacatc gaccgcgtcg agatcgtcat cgacgaggac aacgcctcgc gcatctcgtc
    11881 gttcctcgcc ggcaagtacg acctcggctg ggagaacccg ggcaccatca accggacgga
    11941 ctgggtgcag atcaaggaca cggtgaagca gaagcgtccg gggatccaga cgaccgagtt
    12001 tccctcgaac gtgatgtcgc acatctccat gcggacggat cagaagccct tcagcgacgt
    12061 ccgcgtgcgc cacgccatct cgcacgccat cgagcgccag gggatcatcg atgccacgta
    12121 cgagggcgtc ggcgtcttca acgcggccgt gcccgcggcg ctgaaggact gggcgatccc
    12181 cgtcaatcag ctcggagagg gggcaaagta cttcaagtac gacccggccg aggcaaagcg
    12241 tctgctggcc gccgccgggt accccagcgg ctttcccggg acgctatgct tcacgacgta
    12301 cggctcgacg gtgctcgtcg accagatgca gctggtcgtg aagaacctga aagacgtcgg
    12361 catcgaggcc aagctcgatc agaaggagta cggcgcctac attacgagct gcttctacgg
    12421 caagttcgac tcgatgacct acggtccgca gacgccgttc ctggagcccg acagcttcct
    12481 ctacggccag tactatccgg gcgagccccg caaccagagc cacatcaacg acccggtgct
    12541 ggccgacatg ctggtgcgcc agcgtcggac ctttgacacc ggcaagcgcc gcgaggtcat
    12601 ccacgagatc cagcgctacc tggccagcca gcagtactat gtgacggcgc cctccggagt
    12661 gtacgtggcg gtgtgggacc gcgcgctgaa gaactacggg cccaacgtcg gctacgacta
    12721 cggcgggcgg gtgctgaccg cctggctgga tcgatgagat cggggacccc gacatggccc
    12781 cccaatcggc gagccatgag gcataggtgc tgaggcgcta cttcgccaag cggctgctgg
    12841 tggcggtccc gtcgctgctg atcgcaacgg tgatcgtctt cacgctgccg cggctgatcc
    12901 cgggcgacgt cgtccagctc atgctcgacg agaaggcgta cgccaaggac atcgacgagc
    12961 tgcgcgagaa gctcgggctg aaccggccga tctacctgca gtacttcgag tgggcgggac
    13021 gggtgctgcg gggggacctc ggcgagtccc tgtggacgaa gcggccggtg ctcgaggaga
    13081 tcgggcgccg gctacccatc accctggagc tggccctgct cgcgtcgctg gtcgccgtgg
    13141 ccatctctct ccccatcggc atcgtgtcgg ccacgcgcca ggacacgctg caggactacc
    13201 tggcgcgcag cgccgccatc ctggggctgt ccatgcccgg cttctggctg gccacgctgg
    13261 tcatcgtgct gccggccatc tggtggggct ggcgccccgt ctccggctac gtggagttct
    13321 cccaggaccc cgtcggccac gtcacccagc tcttcctgcc cgccgtcatc ctcggcatcg
    13381 cgtcggccgc ggcgctcatg cgactgacgc gcgggatgct gctcgaagtg ctgcggcagg
    13441 actacgtgcg gacggcgtgg gccaaggggc tgcacgagcg cgtgatcgtc ctcaagcaca
    13501 gcctgcgcaa cgcggtcatc cccgtggtca cggtgctcgg catccagatc gcgcagatca
    13561 tcggtggcac cgtcatcatc gagcagatct tcggcatccc cggcatgagt cgcttcctct
    13621 tcgacgcgat caaccagcgg gattaccccg tcatccaggg cgtcaacctg atcgtcgtct
    13681 cgttcgtcgt gctgatgaac ctgacggtgg acgcgctcta tgccgtcctc gatccgcgca
    13741 tcaggtacta gcgtcggcgg ggggcttcgc cctccgtccg agacctcccc ccacgagctt
    13801 gcgccggcgg agccggcgca cgaacgtggc ggcacccgtc gcgcggtggg cgtggcgcac
    13861 cggcagacgc ggcgcatcac ccggggcctg tggtggttcg cgcgcaagaa gccgctgggg
    13921 gcgctgggcg gggtcatcgt ggtcggcatg ctgctgatga cggtcttcgc cgagcggatc
    13981 gccccctacg actacgacga gaccatccgc ggcgcgcgca tgaggccgcc cggcgccgcg
    14041 cactggctgg gcaccgacaa cctcggccgg gacatcgcaa gccgcatcgt ctacggggcg
    14101 cgggtgtcgg tcaccgtcgg cttcgccgcc atcgggctcg gcatcgtgct ggctaccgtc
    14161 atcggcgtca ccagcggcta cttcggaggc gcctacgaca tcgtcgccca gcgcatcgtg
    14221 gacgcctgga tgtcgttccc ctacctcgtg gtcatcctgt cggtcatggc cgtgctgggg
    14281 ccggggctgc tcaacgtgat cctctccctg gccgtcatcg tcggggccat caactcccgg
    14341 gtcatccggg gggcgaccat cgccaccgcc cagcacgtct acgtggaggc ggcccgggcc
    14401 atgggcgcca gccatggacg catgatcgtg cgccacatcc tgccgaacgt ggccgccacc
    14461 atcatcatcg tgtccaccat cgggctgggc ggcgtcatcc tcgccgaatc ggccctgtcg
    14521 ttcctgggct tcggcgtgcc gccgccctat ccctcgtggg gcgcgatgct ctcgggctcg
    14581 gggcggacct acatgttccg ggcgccgtgg atggcgatct ggccgggcgt cgcgatctcg
    14641 ctcgccgtct tcggtttcaa catgctcggc gacgccgtcc gcgacgtgct ggatccgcgc
    14701 ttgcggggca gttgagcgcg ggagggccac ggcacctcat cgagcgcgcg acagcgacca
    14761 gggcgcggga cgcgccccgg cccggtcggc gcggagcggc ggaaggtctc cgccgcccgt
    14821 ggccgcgtcc tctactgcag cgagccttcc atcgcggtct ggcccgcgtg gagcttcgcc
    14881 accatctcgt tgatgggttg cgtcatctgc gtcatgtggt gcacggcccg ggtgagggcc
    14941 tcggcgatgg ccgcctgctc gccgcggagg tagtcgtgct ggccacgcag ctggttgagc
    15001 tccgcgtgca agtcgttgat ctcccggcgc agcgccccga tctcttcgcg catccggtcg
    15061 cagtcctgct cggccgacac cgccttgtcg cgcagccgct ggttctcgtc gaagacactc
    15121 gggatcaccc gcccgagcag gtactggctc tcctcgatcc accgctcgac ctgctgcttc
    15181 gtctctccgc ccacgtccgt gatactcatg cccgcctggc ccccctctcg ccgattgtcg
    15241 gaggtcccga ggtcgccgtc tcgggtccgc ctccactgtt cacggcacac ggcgacccac
    15301 tggaagcgaa gatcatggtg atagccggcc gggcggcgcc gctccgcgcg ccgtcgctcg
    15361 aggtccggca acagctcgcg ctgacgccgc tcgccctgcc gccgatcgag gaccacgcgc
    15421 gttcccgggt cgccggcgaa gcggcggacc agcgcctcgt gcagcgggcg ctgatcgcgg
    15481 gcgacgacga cgagccagag ggcgggcgtc ccggacgcac acgactcgga acggagtccc
    15541 gtcgcgacga tcacgccgtc ctcgggcgca cgggccagga agaaagcccc aacatcttgc
    15601 gtgggcggca ctctaccacg cgagatttgg tgcgtgtcaa gggcttagtg cgccttctcc
    15661 ttctcggcct tcgcctcctt gatgatcttg tcggccatga aggccggcac ctcctcgtag
    15721 tgcgagaact ccatggagta gccgccgcgg ccgcccgtca tcgagcgcaa cgccggctcg
    15781 tacgtcagca tctccgccat cggcgcctgg gcgcgcaccg ccgccgtctc gccggcgggc
    15841 tccatgccga cgatccgacc gcgccgcgag ttcaggtcgc cgatgacgtc gccggcgttg
    15901 tcggacgggc acgtcacctc gacgttcatg atcggctcga gcaggatcgg gtgggcgtcc
    15961 atgaagacct tctggagtcc catcgacgcg gcgatctgga aggccatgtc cgacgagtcg
    16021 acatcgtggt acgagccgtc gtagagcgtg accttgaggt cgacgatggg atagccggcg
    16081 aggatcccgc gcttcatgca gtcgcgcacg cccttctcca cggacgggat gaagttgcgc
    16141 gggatggcgc cgccgaagat gtcgtcgacg aactcgaagc cattgccgcg cgccaggggc
    16201 tcgacgcgca gccaggtgtc gccgtactgc ccgcggccgc ccgtctgctt cttgtacttg
    16261 ccctgcccct cggcgcgtcc cttcaccgtc tccttgtagg gaatgcgcgg gggcagcagg
    16321 ttcacgtcga cgttgtactt ccgcttcatg cgctccacga ccatctcgac gtggaggttg
    16381 cccacgccgg agaccaggag ttgcttggtc tcggggtcga agtggtagtg gacggtgggg
    16441 tcctcctcgg cgatccgcgc cagcgcgttg gagatcttgt cctcgtcgcc acgcgtcttc
    16501 ggctggatgg cgaaggagat ggccgcctcg gggaacgtga tgcggggcag ctcgaaggcc
    16561 tgggcctcgt cggcgagcgt gtcgccggtg agggtctcct tgagcttggc gacgacgccg
    16621 atctcgcccg gtccgagcgc ctcgacggtc ttgcccgtct tgcccatgag ccacgacacg
    16681 tggctcatgc gctccctcgt cccgcgcgag acgttcagga gctgggactc cgacctcacc
    16741 gtgccgctga agacgcggaa gagcgtgagc ttgcccacgt gcgggtcgct cagggtcttg
    16801 aagacgagcg cggtgacggg ggccttgggg tcgggggctc gcgtgcccgc ctgcttggtc
    16861 ttgacctcga tgcccgggac ctccccgcga tcggccggcg acgggaactc gtggacgagc
    16921 aggtcgagca ccggcgccgt gccgatcccc ctcgtcgcgc tggcgcagag cacgggcacg
    16981 atcttgccct gggtgatgcc gagccggagg gccttgagca tctcggcgtc gccgagaccg
    17041 ccctcctcga ggtacttgtt cagcaggtcg tcgtccgtct cggccgccgc ctcgacgagc
    17101 ttctcgcgat actgcttcgc gcgctcggcg acgtccgccg ggatctcggt ctcgctcgtc
    17161 ttgctcccct cgtggaccac ggccttcatc ttgccgaggt ccacgaagcc gcggaagccc
    17221 gactcctcgc ccaccgggat ctgcagcggg acgatgcggc ccttcaggcg acgctcgagc
    17281 gattcgagcg tgcggtagaa gtcggcgcgc tcgcgatcga ggcggttgac gacgatggcg
    17341 cgtggcaggt tgtactcgct ggcgaacttc cagaccttct cggtctggac ctgcacgccg
    17401 gccacaccgt ccacgacgac cagggccccc tccaccacgc gcaggcccgc gcgagcgtcg
    17461 gcgatgaagt cgccgtatcc cggcgtgtcc acgaggttga tgcggcatcc cttccagtcg
    17521 cagtaggcaa cggcggtgtt gacggagatc ttgcgcttga tctcgtcggg gtcgaagtcc
    17581 gtcgtcgtgc tgccgtcgtc caccttgccg agccggttga tcgcgccggc gccgtagagc
    17641 aacgcctcca cgagcgacgt cttgccgacg ccgccgtgcc ccacgatccc gacgttccgg
    17701 atcttgccga cctcgatcat tcagggcctc ctgaggcaag cgatgcgggt gccagatgct
    17761 atcacttctc ggaggggggt ttcacccccc atcctccact ccgaggctca ctcgagcgcg
    17821 gtgaggtcag ctcgccagga tgctcgtcac gcgggccacg acctcgtcgt cggggccgga
    17881 gaggtcgctc ggagggggga ggggaggggg gtcgttcttg atgccggcgc cggtgaggac
    17941 ggtcacgatg cgggtatcgg cggcgacggc gcctcgctcc ttcatctcga tgagggcggc
    18001 gatggtggcg gccgcctcgg gggccgtcca gacgccgtcg agcctggcga gcagtcgctg
    18061 ggcgtcccgg atggccgtct ccgacacggc gatggcctcg ccaccgctct cgcggacgat
    18121 gcggagcatc tggcgcccgg cgaagggggc cggcacccgc agccccgccg catgcgtcac
    18181 cggccgctcc cacgcggtgg tgacctccgc gcggtcgcgc cacgccttca ccaccggggc
    18241 gcagccctcg gcctgcacgc tcacgaatcg cggcccgcgc cccgagatcc acccgatcgc
    18301 gcgcagctcg tcgaagccct tcgggatgcc gaccagtccc gtgccgccac cggtcgggta
    18361 gagcagcacg tccggcagcg tccagccgag ctgctcggcc agctcgaggc ccatcgtctt
    18421 cttgccctcg agccggtacg gctccttgag cgtcgagagg tcgaaccagc cgatccgcgg
    18481 cgcgaccttc gccacgatgc ggcctgcggt ggcgatggag ccctcgatcg tgaacacgcg
    18541 cccgcccgcg atgagggctt ccgcgatcgc cgccggcggt gtcccccgcg gcacgatgac
    18601 gccgcacggc agccccgcgc gcgcggcgta gagcgcggcg gcaccaccgg cgttgcccgc
    18661 cgacgggatc aggaagccac gcgcgccgag cgtgcgcgcc ctggtgatcg ccatgccgag
    18721 gccgcgcgcc ttgaaggtgc ccgtcggatt ctggccttcg tccttgcccc agaggtggcg
    18781 cagcccgagg tgcgccgcca ggcgcggcag cggcagcagg ggcgtgcctc cctcgccgag
    18841 cgtcaccggc tcctcgccgt cgtccagcgg ggtcagctcg cggaagcggt acatgcccgg
    18901 cggccgcacg cggagcgcct ccttcgtcac gctgcccctc acgcggtcga cgtcgtagcg
    18961 cgcggccagc atctgcccgc actgctcgca gacggtgagc ggccgtttcg cgtcgtgccg
    19021 gcgaccgcac acggtgcact cgacgtgcgt cacgaagctc atggggttct caggatccgc
    19081 gggccggagc ggctacgcca tcacctgccc gccgttgacg tcgagcgtcg cgccggtgac
    19141 gaagctggcg gcatcggacg ccaggtacag gatcgcgtcg gcgatctcgg acggctcgcc
    19201 gagccggcgg agggggatcg tctgctcggc caggcgccgg ctctcctcgg gcgtccgcag
    19261 cgctttcacg cgctcggtcg ccgtcgtgcc cggcgccacc gcattgacgg tgatgccgtc
    19321 gggaccggcc tcgagcgcca ggtggcgagt gaagcccacc acgccggcct tggccgcggc
    19381 gtaatgcgag gtgacgcgca cggccccgcc gcgggccacg accgaggcga cgttgacgat
    19441 gcgccccgag cgctggcgtt tcatcaccgg cagcacggca cggcagcaga cgaacgcgct
    19501 cgtgagattc gagcgcagga tcatctccca ctcctcgtcc gtgatgtcct cggtggcgcg
    19561 gatcgccgcg aagccgcccg cgttgttcac cagcacgtcg atgcgccccc agcggtcgac
    19621 gatggcctcg acgacctcgc ggatccgagc gggtacggcg gcgtccgctc cgaaggcgag
    19681 cgtctcggcg ccggactcgc gcaggcgcgt ggccgtggcc tcggcgccgg gcccgtccag
    19741 gtcgagcgcc gccagacggg cgccctcgcg cgcgaacgcc tcggcggtga cggcgccgat
    19801 gccgcgcgcc gcgcccgtca cgatcaccac ccgtcccgga aacctcatgg cgccaccacg
    19861 tggttctgca gcacgccgat gcgctcgacc tcggcctcga gcgtgtcccc gaccctgagg
    19921 aactcgccca gcgcggcacc cacccccgcc ggggtcccgg tggcgatgac gtcgccgggc
    19981 aggagcgtga acccctcgga gagcacgctg atgcactcgt ccacggggaa gatcatcttc
    20041 gacgtgtggc tcgcctggcg cgtctcgccg ttcacgcgca gcgtgatggc cagcgcctgg
    20101 gggtcgggga tctcgtcggc ggtcacgagc acggggccca tcgggcagaa ggtgtcgagc
    20161 gtcttgccct tgaaccactg gccgtggcgc ttctggaggt cgcgcgcggt cacgtcgttc
    20221 acgacggtgt agccgaacac gtgctcgagg gcgcgctccc gcgggacgtc gcggcccgcc
    20281 gtgccgacga ccaccgccag ctccacctcg tagtccagct ccttcgtgac cgcgtggtgg
    20341 acgatcttgc cgcccggcgc gatcaccgat tcgggcacct tggtgaagta cacgggatgc
    20401 gacggggcct cggcgccgcg ctcggcggcg tgctccttgt aattgcgccc gagacagaac
    20461 acgttgcggg cgggtcgcgg gatgggcgcc tcgaggcgag ccttggcggc ggcgaggccg
    20521 agcctgcgct cgacgaggcc gtccacgccg tggcgctcga gcaggccctc gccgtgcgcc
    20581 agcgcctcgc gcgcgcggcc gagcgcttcg gcgccgccgg cgatcagctc gagcatcgtc
    20641 gtggggaagc cccctcgccc gtgccggacg gcgccgcgct cacgggcgag atcgacggcc
    20701 agcgccccga ggtcgagcac ggcgccgggc gtcgccgcgc cgatcttcgg ccgggaaggg
    20761 cctttgcggt acgtcaccag acgcatggct cactgtcctc gtgctcaaat agtctgccgc
    20821 atcaccgtcc tcgacggcag cgtgatgcca cgctcgcggg ccatctgctc cagttcgggc
    20881 ggcagggcga accggacgtt ctcgtcgacg acgtggacct cgcgcacgtc cgagccgatg
    20941 cgccgcagcg cgtccaccgc ctcccgcacc agcacttccg gcgccgaggc gcccgcggtg
    21001 aggccgaccc gccgggcgcc ggccagccac gcgggctcga tgtcgcgcac gtcgttgatg
    21061 aggtacgcgc gagtcccggc ggagcgagag acctccacga gccggatcga gttcgagctg
    21121 ttcttggcgc cgatcacgag cagcacgtcc acgtcgccgg ccacggtctt gaccgccgcc
    21181 tggcgattct gcgtcgcgta gcagatgtcg tcccgtgacg gtccgacgat cttggggaat
    21241 ttctcccgca gggcctcgat cacgtcgcgg gtgtcgtcca cggacagcgt cgtctgcgtg
    21301 aggtaggcga ccttgtcggg gttcggtacc cgcagctgct cgacctcgga gacgctgtcg
    21361 atgacgaaga tgcggtcggg agcctccccc agcgtgccga tgacctcgtc gtggtcttcg
    21421 tggccgatga ggacgatcga gaagtcctcg cgcgcgtagc ggatggcctc gaggtgcacc
    21481 ttggtcacga gcgggcaggt ggcgtcgatc acgcgcagcc cgcgtcgggc cgcgtcctcg
    21541 cgcaccgccg gcgagacgcc gtgagcgctg aagaccgccg tggcccggtc cgggacatcg
    21601 tccagctcgt ccacgaagat ggcgcccttg gcgcgcagct tctcgacgac gtggccattg
    21661 tggacgatct ccttgcggac gtagaccggc ggctgacaca cctcgagcgc cagctccacg
    21721 atgtcgatcg cgcgctcgac gcccgcacag aagccgcggg ggctggccag gacgatctcg
    21781 gcgatgggcg tgctcatggc tcccagccta cttgaagaag aacgaaacgc ccaccacgcc
    21841 ggtgtgagcc tcccagccgc ggttgggcga gtcggtgttg ccgttggagt tgtgcgtgaa
    21901 gcggtagccg gcgtacaggg cgtggtggtc ggtgatgaag accgaggcgc cgatcccggc
    21961 gatcagcagg atcgaggcgt ccgagtcgat ctcgttgatg tcgaggttcg tgccgccgac
    22021 ggcggccgcc ccctcgaggt agggcacgaa gcggcccagc ctgaggaagt ggtagcgggc
    22081 caccgccgcg aggcccgcgt agaacgcgtc gtcgggattc aggtagtgca tgtacagggg
    22141 ttccaggccg atctcgaacg ccgagcgcag cacgctgtcg ggggcgatga tattgaaggg
    22201 cagcaacgac cagcgcgctc ccacggacac gaattcgaga tcggtgaagg tccggaagtt
    22261 ctccaggttg acctggttgc cgtaggctcc ctccagcgac cagacccagg tccccttccg
    22321 ccagacctcg gacgaatcgt aggtcacccc gggcacggga ccggcgagga cggcgacgac
    22381 gagcaggacg ccggcgagga cggtcggcga tccgggccgg cggctcatgg ctggacgagc
    22441 accttcagcg cctcgcctcg cgacatgagc tccagcgcct cgccgacccg gtcgagcccc
    22501 atccggtgcg tgagcagccc atccgggttc accgtctcgg cttccaggag ctccacggcc
    22561 cggcggatga gctcgggcgt gtgatggaat gctcccacca gcgtcagctc ttcgtagtgc
    22621 gctcgccgag tgtcgagagc cactgtcgtc cctggcgcac aacccccgaa gaataccacg
    22681 gcccctccgc ggcccacggc atccatcgcc tgcgaccacg cctcgggccg gccggtggcg
    22741 tcgatcgcga cctcgggccc ccggcctccg gtcatcgtcc gcagctcggc cacgaggtcc
    22801 ggcgacgccg tcgcgtccac gcactcggcg atgccgagcc cccgcacgcg gtcgagccgc
    22861 cagccgcgct tgcccacgac gatgacgcgg gccttccggg cggcggccac catggcgagc
    22921 aggcagccga gaggcccgtg acccagcacg accaccgtct gccccgactc cagccgcgcg
    22981 cgctcgatgc cgagtagcgc acaggcgagc ggttcggcga aggcggctcg cgccgcggac
    23041 agcgtggcgg cgagcctcac gacgttcttc gccaccaggc gtggcggcag ggcgatggcc
    23101 tccccgtacg cgccgttgac gaagagcagg tcctcgcaga ggttggggcg ggccgcccgg
    23161 cagcaggcgc acgtcccgca gggcgccgag ttcgccgcca ccacgcgatc gccctcacgc
    23221 agccccggga cgccgccgcc gacggcggtg atggtgccgg ccagctcgtg gccgaacacg
    23281 gtgggcacgc gcgggatcat gaccggatga cctcgccgca gcgtcttgac gtcggtgccg
    23341 cacgtgagcg cggcgtcgat gcggacgacc acctcgcccc gtgcgggctc cggccacggg
    23401 agctcctcga agcgcagatc gccgggaccg tggaagacga cggccctcac ggcgtcacgt
    23461 agaccttcag tgcctgcgcg gtgcgcatca gctcgacggc ctcgtcgagc cgctcgagcg
    23521 ggcggcggtg cgtgtagagg ccgtccagcc gcacctggcc cttcgtcacc aggccgaagg
    23581 cctccgcgag atcggccggg gacgacgagt acgtggtcgt gatcgtcagc tcgcggtggt
    23641 agagcgtgtc gagcgccacg ggcagcgcat cgccggggcc ccccgcgaag tagtggatgg
    23701 cgcccccgtc gcggacggtc cggaccgccc actcgagcac tccggcggcc ccgccggtga
    23761 tgagcacgag gtcggcgccg cgtcccccgc tgagtcgcgg caccgcggtg gcggcctcgt
    23821 cgacatccat caccgggagg ccgagcgccc gagcggactc cacacggtga cgctgctggt
    23881 ccacgccgac caccgcggcg cccgcgcgtt ccaggagctg cacgaacagg cagccgatcg
    23941 agccgagccc gacgaccacg gcgctgtccc ccggctcgac cgcggcacga cgcaccgccc
    24001 gcaggcagca cgccagcggc tccacgaacg acgccgtctc gtcgctcacg tgagggggca
    24061 cacggaacgt cgcgtgcgcc acgttcggag cgggcacgcg caccagctcg gcgaacccgc
    24121 cggggtcgag atggctcgtc ttgaaggccg ggcacatcga gtggctgccg cggcggcagt
    24181 agtggcagcc ctcgcacggc acgtgatggg cggccaccac gcggtcgccg gcgccgaagc
    24241 gggtttcggc gccggcctcc agcacgtcgc caaccagctc gtggccgagg acgagcggcg
    24301 tccgcgtgcc gcgcgccccg atcttcgcga tgtccgagcc gcagaggccg cagccgcgca
    24361 gccgcagcag cagctcgcca ggtccgatcc ggggcgcggg ccaatcctcg gcgacgatgc
    24421 ggccgggggc gatgaggacg gcggccttca cagcgcggcg cgcagcagac gcgcgccggg
    24481 caaccgcgcc gccgcccagc agccgagggc gatcgcggcc ttccggcggg ccggcaccgt
    24541 aacgcgctca ccgaagacct ggaaccggcg cgcctcgatc tcccccaaca gcgcgtggta
    24601 gatgcggccc atgatctccg cggccaccag cgagcgggcg tcgacgcgcg gaaaggccgc
    24661 gcgcgcgcgg ccgtagaact cacgagctcg cgcggcctgc cgctccatga cggcgacgaa
    24721 ccccggcgtg tagcgggcgg cgcgcagatc gtcgacgctc acgccgcagg cgcggagatc
    24781 ggtctgcggc agatagacgc gcccggcgcg ggcgtccacg ccgacgtcgc gcaggatgtt
    24841 cgtgagctgg agcgcgagcc cgaggctcac cgcgtactcc cgggcacgcg ggtccgtgta
    24901 gccgaagatc tcgatggacg ccagccccac ggccgacgcc acgcggtagc agtaggggta
    24961 gaggtcctcg gccgtctcga acgtgacgcc gtccacgtcc atcgccacgc cttcgatgat
    25021 ggcctcgagc gctgcgcggg gaatcggata gcgctgcacg gccgcgcgca gctgccgagc
    25081 gatgggctgt tcgggcgcct cgccggcggc gtagcagcgg gcgacctcgc gccgccaggt
    25141 gtcgagctcc gcccgcatcg ccgccggcgt ggccccgcgc tcggacccca ggtcggcgat
    25201 gtcgtccacc gtgcggcaga aggcgtagac ggcgtagagc gcgtcccgcc gcgggcgcgg
    25261 caggaccagg aacgcatagt agaagttcga ccggctgcgc cgcgtcagcc gcgagacgag
    25321 gtcggcgggg ctcactgcgc ggccgccccc ggccgcgtga cgacacgcgc atacccgtgc
    25381 tcggcgaacc aggtgacggc gtcggccagc gcctc
//
LOCUS       DATIAZ010000243         5395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_196060, whole genome shotgun sequence.
ACCESSION   DATIAZ010000243 DATIAZ010000000
VERSION     DATIAZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196060"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VLF19_08645"
     CDS             <1..265
                     /locus_tag="VLF19_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93362.1"
                     /translation="TLLVIEPDAIVYVKLRALDGPLPVEGAGALGLGFFLLAVEDVLE
                     DAAADWEGGMRITRAIEYAPAPTLEEPWARVTLAALAEPRARA"
     gene            317..643
                     /locus_tag="VLF19_08650"
     CDS             317..643
                     /locus_tag="VLF19_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93363.1"
                     /translation="MATMKAPGYRLAPALLLFALLALAHAQHGLALIGTVWRIAALSW
                     DNPATQLIEEVASQAVPHVDPVRSLRDRIVAALADVAGALPPDDGACSLAPALTACGT
                     RAPPLA"
     gene            730..894
                     /locus_tag="VLF19_08655"
     CDS             730..894
                     /locus_tag="VLF19_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93364.1"
                     /translation="MYEILGQIVGGLVAAAAFVGLVVYRTWQERKERRGLQQRGRGAE
                     PETAAPRHAG"
     gene            904..1125
                     /locus_tag="VLF19_08660"
     CDS             904..1125
                     /locus_tag="VLF19_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93365.1"
                     /translation="MDSTLSRVSLGVVVIALIGLVVLYHVWIERRYRRQGPPAAAGAP
                     GASKESDRPDQTAVRAAPAPDKPPVHRRD"
     gene            complement(1324..1740)
                     /locus_tag="VLF19_08665"
     CDS             complement(1324..1740)
                     /locus_tag="VLF19_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93366.1"
                     /translation="MSSRAQDRTASAEPRQALVTLTHVIYGLHAFSLVTGIIGAATVV
                     GAFLTGWPSIIAVILNYAKRSEGRGTWLESHFRWQIRTFWFGLLWVTLCLLFVVATFG
                     IGMLIAWLPLGIVGLWFIYRVAHGWLRLIDRRPMYE"
     gene            complement(1753..2832)
                     /locus_tag="VLF19_08670"
     CDS             complement(1753..2832)
                     /locus_tag="VLF19_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSE93367.1"
                     /translation="MRRSALHLPVSLFVALVLVSGCGAPLTKADTRTGPVAAPPELAR
                     FNELLADLSERLKPALVHVRVRREAGQEEGRDPQEPRRSSGSGFVIEQDGLIVTNAHV
                     VEGVQTAQVRLFDGRRFTARVIGRDNRVDLALLRLEGVSGLPTLSLGDSNRLRVGEFV
                     LALGHPFGLEHTVSFGIVSRKGSPLIVAAPGFDFIQTDAAINPGNSGGPLVSMAGEVV
                     GVNSMAARNGSIGFAIPANLVKMLVPQLAANGRVDWGWLGVSITDVGDDDLGRLRLRE
                     ARGVLIRSVVPGDPAAQGGVKADDVLLSLDGTRLETPRDLQRVVATTPVGKKVRVTLW
                     RDGREQDVEITIGKYRDPEEHSEKK"
     gene            complement(2916..3404)
                     /locus_tag="VLF19_08675"
     CDS             complement(2916..3404)
                     /locus_tag="VLF19_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93368.1"
                     /translation="MSNQRSRRTRYATRRALGIVTAAWLAVELGGGSPLAGGALGGSS
                     LGSGPLGGGPSGAGARGETSTYGRGTLGPRPIDQLDRAVRQPPPSVPPAPAPRTDMIW
                     VPDRYLDRPEGSLHVPGHWEQRLNPQEHYVPPLYVCNRSTGECAQIPAGVRSPPETRP
                     GP"
     gene            complement(3401..3853)
                     /locus_tag="VLF19_08680"
     CDS             complement(3401..3853)
                     /locus_tag="VLF19_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HSE93369.1"
                     /translation="MLTISDSKTPETDTSGALIRSLLEGGGHRVAGSSIVRDEPVDVQ
                     RVIRGAVTDPAVQVIVMTGGTGITSRDSTYEAVEELLDKRLPGFGELFRMLSYQEIGA
                     AAMLSRAQLGVHQGRILVSLPGSPNACRLALEKLLIPELGHLLREVNR"
     gene            complement(3916..5292)
                     /locus_tag="VLF19_08685"
     CDS             complement(3916..5292)
                     /locus_tag="VLF19_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSE93370.1"
                     /translation="MAHATEREPAKAAGPLRLHRLSVEGVNQCVHCGLCLPYCPTFSE
                     LGTEMDSPRGRIFLIKSLAEGRVALSDSVVDHLSLCLDCRACETACPAGVPYGRLIEA
                     AKAEIERQRPGSLLRRAFRWANFGMLLGRPGALRAAAAALRVYQASGLQGLVRATGLA
                     RLLPGTLPAWEALLPSLPAASERRPLPPLIAAEGERRARVALLAGCVQSVVFGAHNRA
                     TARVLAKNGCEVVVPRAQGCCGALNAHGGDHGRAVEMAKGTIEVFERAEADAIVVNTS
                     GCGAHMKGYGSLLSDDLAWAERARRFADRVQDVSEFLARTPLRGPLQPVPMTVTYHDP
                     CHVVHGQKIRSQPRALLAQVPGLRVVDLPESDWCCGSAGIYNLTQPEMADRLLRRKIR
                     HVLATGAEAVVTANPGCILQIQAGLEAQQSKTRVLHIVEILDRAYTSERASSSSLGIA
                     PAKPGREP"
BASE COUNT          770 a         1946 c         1934 g          745 t
ORIGIN      
        1 gacgctgctg gtgatcgagc ccgacgcgat cgtctacgtg aagctgcgcg cgctggacgg
       61 tcccctgccc gtcgaaggcg ccggcgcgct cggcctgggg ttcttcctgc tcgcggtgga
      121 ggacgtgctc gaagacgccg ctgccgactg ggagggcggc atgcgcatca cgcgcgcgat
      181 cgagtacgcg ccggcgccga cgctcgagga gccgtgggct cgggtgacgc tcgccgccct
      241 cgccgagccc cgcgctcgcg cctgagcagc gcggcccttg acgtcaccgg cgcccggcac
      301 gtacattcgc gccatcgtgg cgacgatgaa ggcgcctggc tacaggctgg ctcctgccct
      361 cctgctcttc gcgctcctgg cgcttgccca cgcccagcac gggctggcgc tgatcgggac
      421 ggtctggcgc atcgctgccc tctcgtggga caaccccgcg acgcagctca tcgaggaagt
      481 cgcttcccag gcggtgcccc acgtcgatcc ggtgcggtcg ctccgtgaca ggatcgtcgc
      541 cgccctcgcc gacgtcgccg gcgccctacc gcccgacgac ggcgcgtgct cgctcgcccc
      601 ggcgctcacc gcctgcggca cgcgagcgcc ccccctcgcc tagctcgcca gagttgacaa
      661 gcggttggat gccgtcccct gcggccggcg tccgtcctgg tgtgctcatc gcgatccggg
      721 gggtgggtca tgtacgagat cctcggtcag atcgtcggtg gcctcgtcgc cgcggcggcg
      781 ttcgtgggcc tggtcgtcta tcgcacgtgg caggagcgca aggaacgacg aggcctgcag
      841 caaagggggc ggggcgccga gccggagacg gctgcaccgc gccacgctgg ctgagccggc
      901 gtcatggaca gcacgctgtc ccgcgtgagc ctcggcgtcg tcgtgatcgc tctgatcggc
      961 ctggtggttc tctatcacgt ctggatcgag cgccggtatc gccgccaggg accgcccgcg
     1021 gccgccggtg ccccgggggc cagcaaggaa agtgaccgcc cggatcagac cgcggtccgg
     1081 gcggcccccg cgccagacaa gcccccggtc caccggaggg actgaccccg gcgatcgagt
     1141 actgggctgc cggcctccga cacggtcggc ccgcggccca cgagatcgtg gaccaggaca
     1201 gcacgggtcg gtgcatcgag gcccgcgccc gccgggcgcg actttgccgc gcctgcgtcc
     1261 gcgtgtcgcc ccgcgttgat gcagcctcgg cgcagcggca ctttcgagag ccaggaggcc
     1321 gtgctactcg tacatcggtc ggcgatcgat cagccggagc cagccgtgcg ccacccggta
     1381 gatgaaccac agcccgacga tgccgagcgg aagccaggcg atgagcatgc cgatgccgaa
     1441 cgtggccacc acgaagagca ggcagagcgt cacccacagc agcccgaacc agaacgtgcg
     1501 gatctgccat cgaaagtgcg actcgagcca ggtcccgcgc ccctcgctcc gcttcgcgta
     1561 attcaggatg acggcgatga tcgagggcca gcctgtgaga aatgcgccca ccacggtggc
     1621 cgcgccgatg attcccgtga ccaggctgaa ggcgtgcaga ccatagatga cgtgcgtcaa
     1681 ggtgacgagc gcctggcgcg gctcggcgga ggcggtcctg tcctgggccc gagacgacat
     1741 cagttgccgc ggctacttct tctcggagtg ctcctcgggg tcgcggtact tgccgatcgt
     1801 gatctcgacg tcctgctccc tgccgtcgcg ccagagcgtg acgcggacct tcttgcccac
     1861 cggggtcgtg gccaccacgc gctgcaggtc gcgcggggtc tcgagcctgg tgccgtcgag
     1921 cgagaggagg acgtcgtcgg ccttgacgcc cccctgcgcg gcgggatcgc cgggcacgac
     1981 ggagcggatc agcacgccac gggcctcgcg cagccggagc cggccgaggt cgtcgtcgcc
     2041 cacgtccgtg atgctgacac cgagccagcc ccagtccacc cgtccgttgg cggcgagctg
     2101 cgggaccagc atcttgacga ggttcgccgg gatggcgaag ccgatggagc cgttgcgcgc
     2161 cgccatgctg ttcacgccga cgacctcgcc ggccatgctc acgagcgggc cgcccgagtt
     2221 gccagggttg atcgcggcat cggtctggat gaagtcgaag ccgggcgcgg cgacgatgag
     2281 cggcgagccc ttgcggctca cgatgccgaa cgagaccgtg tgctcgagcc cgaaagggtg
     2341 gcccagggcc agcacgaact cacccacgcg gagccggttc gagtcaccga gggacagcgt
     2401 gggcaggccg gagacgcctt cgaggcgcag cagagctagg tccacacggt tgtcccggcc
     2461 gatgacacga gcggtgaagc ggcgtccgtc gaagagccgc acctgcgctg tctgcacacc
     2521 ctcgacgacg tgggcgttgg tgacgatgag gccgtcctgc tcgatcacga agcccgagcc
     2581 cgagctgcgg cggggctcct gtggatccct gccctcttcc tgacctgcct cgcggcgcac
     2641 gcggacgtgc accagggccg gcttcagccg ctcggagagg tcggccagca gctcgttgaa
     2701 gcgcgcgagc tccggcggtg ccgcgaccgg ccctgtccgc gtatcggcct tcgtcagcgg
     2761 agcgccgcag ccggacacga gcacgagagc cacgaagagg ctgacaggca ggtgaagcgc
     2821 cgaccgtcgc atataggtcc tcctttgccg caataacagc agtatagcgc ctgccgcaaa
     2881 cgggcgcggt gcgcgcccat ccatgccgca gcgcgttaag gacccggtcg cgtctccggc
     2941 ggggaacgga cgccggccgg aatctgggcg cactcgcccg tggagcggtt gcagacgtac
     3001 aggggcggga cgtagtgctc ctgggggttg agccgctgct cccagtgccc agggacgtgg
     3061 agcgacccct cggggcggtc gagatagcga tcaggcaccc agatcatgtc cgtgcgcggt
     3121 gccggggccg gcggtaccga cggcggcggc tggcgcaccg cgcggtcgag ctggtcgatg
     3181 ggccgtggac cgagcgtgcc acgtccatag gtggaggtct cgccgcgagc gccggcgcca
     3241 gacgggcccc cgccgagcgg gccgctcccg agggaactgc cgccgagggc gccccccgcg
     3301 agggggctgc cgccgccgag ctcgacggcg agccacgcgg cggtgacgat cccgagagcg
     3361 cgcctggtcg cgtaccgcgt ccgccggctg cgctggttgc tcaccgattc acctcgcgga
     3421 ggaggtgacc cagctcgggg atcagcagct tctccagcgc gagccggcag gcgttcggcg
     3481 agcccggcag cgacaccagg atccggccct ggtggacgcc gagctgggcg cgcgagagca
     3541 tcgccgccgc gccgatctcc tggtaggaca gcatgcggaa cagctctccg aagccgggca
     3601 gtcgcttgtc gagcagctcc tccaccgcct cgtaggtgga gtcgcgcgac gtgatgccgg
     3661 tgccgccagt catgacgatg acctgaacgg cgggatccgt cacggccccg cggatcacgc
     3721 gctggacgtc caccggctcg tcccgcacga tcgaggagcc cgccacgcga tgccccccgc
     3781 cctcgagcaa cgaccggatg agcgcgccgc tcgtgtccgt ctccggcgtc ttgctgtccg
     3841 agatggtcag cacgtagcac cctacggcct tcggcgcggt cgccttgtgc tcatccggag
     3901 tcgccgagcg ccgagtcacg gttcgcgccc cggctttgcc ggcgcaattc cgagggagga
     3961 cgaagacgcc cgctcggacg tgtacgcccg gtcgaggatc tcgacgatgt gcagcacgcg
     4021 cgtcttcgat tgctgggcct cgagcccggc ctggatctgc aggatgcagc cgggatttgc
     4081 ggtcaccacg gcctcggcgc ccgtggccag cacgtgacgg atcttgcgcc ggagcaggcg
     4141 gtcggccatc tcgggctgcg tgaggttgta gatgcccgcg gagccgcagc accaatccga
     4201 ctccggcagg tccacgaccc gcagccccgg cacctgggcg agcagcgcgc gcggctggct
     4261 ccggatcttc tgcccgtgca cgacgtggca ggggtcgtgg taggtcacgg tcatggggac
     4321 gggctggagc ggccctcgca gtggcgtcct ggccaggaac tcggagacat cctgcacccg
     4381 gtcggcgaag cgccgcgcgc gctcggccca ggcgaggtca tcggacagga gcgagccgta
     4441 gcccttcatg tgggcgccgc atcccgacgt gttgacgacg atcgcgtccg cctcggcgcg
     4501 ctcgaagacc tcgatcgtgc ccttggccat ctccacggcc cgcccgtggt cgccgccgtg
     4561 ggcgttgagc gcgccgcagc agccctgcgc ccgcggcacc accacctcgc agccgttctt
     4621 cgcgagcacg cgcgcggtcg cgcggttgtg ggcgccgaag acgacggact gcacgcatcc
     4681 cgcgagcagg gccacgcgcg cccgccgctc gccctcggcc gcgatcagcg ggggcagcgg
     4741 ccggcgctct gacgccgccg ggagcgacgg cagcaaggcc tcccacgccg gcagcgtccc
     4801 gggcagcagt cgcgccaggc ccgtggcgcg gacgagcccc tggagcccgc tggcctgata
     4861 gacgcgcagc gcggccgccg ccgcgcggag cgccccggga cgccccagca gcatcccgaa
     4921 gttcgcccag cggaacgcgc ggcggaggag gctgcccgga cgctggcgct cgatctccgc
     4981 cttggccgcc tcgatgagcc gcccataggg cacgccggcc gggcacgcgg tctcgcacgc
     5041 acggcaatcg aggcacagcg acaggtggtc gaccaccgag tcgctcagcg ccacgcggcc
     5101 ctcggccagc gacttgatga ggaagatgcg gccgcgtggc gagtccatct cggtgccgag
     5161 ctcggagaac gtcgggcagt acggcaggca cagcccgcag tgcacgcact ggttcacgcc
     5221 ttccacgctg aggcggtgga gcctcagtgg ccccgccgcc ttcgccggct ctcgctccgt
     5281 cgcgtgcgcc atcgcggtca gatgcccccg atgaaccggc cgggattgaa gatgccctgc
     5341 gggtcgagct gcgccttgat ccgctgcatg atgcgcccgg cggctccggc gtcgt
//
LOCUS       DATIAZ010000244         8585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1907174, whole genome shotgun sequence.
ACCESSION   DATIAZ010000244 DATIAZ010000000
VERSION     DATIAZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907174"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1373
                     /locus_tag="VLF19_08690"
     CDS             132..1373
                     /locus_tag="VLF19_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93371.1"
                     /translation="MGKKLIVALLAVLVAVAVVSVGPAPAQKGPIKIGFLAPMTGGAA
                     QIGKDMTNGLSMWLDENKWTIAGRKVEVIVEDTQGQPNIALTKLQKLVESDKVQVLAG
                     GLFAHVGYALAPKVDQFKIPMLYPVMAADDLTQRKPTKWVVRTGWTSSQPSHPFGEYA
                     AKTLGYKKVVTIGIDYAFGWEVVGGFQKTFEENGGQVVQKLWAPLNTADFAPYLSQIR
                     RDADAVFALMVSISSLRFPKQYENAGLKAKLPLIGGGTTFDEFVLPSLGDEAIGGISP
                     LIYSAALDTPVNKRFVAEYRKKYGKVPSYYSETCYTSGRWINEAAKAVNGDVENREKF
                     LEALRKVEIPDAPRGPVKLDAHGNPVQNIYVRKVEKKDGELWNTVVQVYPNVSQFYKY
                     KPEEFLKQPVYDRNFPPCKFC"
     gene            1394..2146
                     /locus_tag="VLF19_08695"
     CDS             1394..2146
                     /locus_tag="VLF19_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93372.1"
                     /translation="MQAATTALTLSRVSKAFGGLHAVDAVSLEVRAGERRAIIGPNGA
                     GKTTLFSLISGEQAITGGSIELFGRDITRLAPYRRAALGLSRTYQITNLFPRLSALDN
                     CLLSVQALTPVKLHLHRALDRYPDLFERAREMLAAVGLEARADQAVRDLSHGEQRQLE
                     VAMALGGSPKLLLLDEPTAGLAPAESQMMTALLKKLDPAITLLVIEHDMDVAFALTDR
                     ITVLHYGRVVADGLAEQVKSDPLVQEIYLGTA"
     gene            2157..3197
                     /locus_tag="VLF19_08700"
     CDS             2157..3197
                     /locus_tag="VLF19_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSE93373.1"
                     /translation="MVLHGLGQPLRLEDVPMPRIGPNDALLRVRATGVGLTVVIMTAV
                     PGRVTSFPRIPGHEVAGEVVEVGGEVRNVRPGERVTCHFYLTCHACAYCRSGRETLCT
                     AFRGNVGMACDGAYAEYMAVPAANLVRIPDGVTDVEAAVAADAICTPYHACREEARVG
                     PGDRVLVVGAGGGVGIHMVQMARLCGGWVIGADVDEAKLAAAREHGADAAVDARGGDL
                     AAHVLTATGGRGVDAVIDTVGSRETLEASVRSLAIAGRLVIIGHRPKPVFGVDATFGV
                     DPGLMLNRMLEIHGSRYVTLAEIEQTLELLRQRRLRAVVTRTFPLEGAEEAHGLLRTN
                     ALVGRAALLVAP"
     gene            3197..3892
                     /locus_tag="VLF19_08705"
     CDS             3197..3892
                     /locus_tag="VLF19_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93374.1"
                     /translation="MLDVRDIHTYYGDSYVLQGISLRMERGQAVAILGRNGMGKTTLI
                     RSIVGFTRPRRGQIVFKERDITGWPSNRSVALGLGLVPQGRRVFPSLTVLENLAVASA
                     QRGGGGWTVDKVMDLFPRLRERAQNRAGKLSGGEQQMLAIARALMTNPELLVMDEPTE
                     GLAPLLVREVGRVIESLKASGLSILLVEQNLPLALRVADHVHVLSRGRIVHSSTAAAL
                     AGNHEIKARYLGL"
     gene            3903..4784
                     /locus_tag="VLF19_08710"
     CDS             3903..4784
                     /locus_tag="VLF19_08710"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93375.1"
                     /translation="MKRIGIVGVGLLGSAVASRLLAARFEVAGYDPVPKQLEALRPRG
                     LCPAGSIGEAAAGADAVFVILPSLETVDAAVRGPGGLVETLPRGGVILQMSTISPALT
                     RTLAAASAAKGVGFLDTPMSGTSAMVERGDCTVYVGGDPTLAERVRPVFDAIARKTVH
                     VGEAGHAMLAKLATNLLVGLNTAAAAEALVLGAKGGLDPAHLLAILRESAAASRMLDV
                     RGPLMVERKFDAQMKCDLFLKDFKLMLDEGMRLGVALPLTSLTQQLTTATVAAGRGGE
                     DLAALIKTIEDLAGMGD"
     gene            4787..5848
                     /locus_tag="VLF19_08715"
     CDS             4787..5848
                     /locus_tag="VLF19_08715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE93376.1"
                     /translation="MRFGTFFFFQAPPGVRHDEIIHNELEQMEWTEELGFDEVWLTEH
                     HFIDYGLAVDPASLAAAAASRTRRVRIGLAAAILPFHHPIRLAEQMALIDIISKGRLD
                     VGVGRGNRPAEFRGYHVPQQESRDRFDETVELMVRAWTEEHVSHQGRFFTVPEARVIP
                     KPVQRPHPPLYQVCVTKDGIENTALRGWPMLNSVLFGPVDQLVGNRDIYLDTLRKAGR
                     SEDEVKALLRRWGVSRQIYVADSDARALEDARPYEMWYQDSFRRFVVPDRIEDAHPTL
                     QPGFRAMADRLSKVTWEGLVAETLAFGSPDTVARHIEHMRSIGVGQVLCWMNFGGLPQ
                     AKVRRSMELFAREIMPRFR"
     gene            complement(5939..7096)
                     /locus_tag="VLF19_08720"
     CDS             complement(5939..7096)
                     /locus_tag="VLF19_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HSE93377.1"
                     /translation="MTRLDPARADVVGSLLRPQYLKDARQAVREGRLDAGRLAAVEDR
                     AVREAIALQESAGIDVVTDGELRRNSWVVTIPLREEGAAHAPLAGYEFLPAGPGWWSL
                     WKEPDGRPAQVWTRDTRPFVTQPLRVVRDIVADEYTFLKANARARTKFTIPAPSWHRI
                     FWHPEHSRAAYPTADDFLRAVAAYLRDEVVKKIAALGGDYVQMDAPNYAQWHVDRDNR
                     AAFESWGHDMDRELVADAEIDDTVFEGVRGITRAIHICRGNAPGGRWLANGGYERIAG
                     QVFPRLTRYDRLLLEYDTPRAGDFGPLVHVRPGTEVVLGLLTTKRGALEDGAAVESRI
                     REATRHVPLERLAVSPQCGFASGEAGNPLTPAEQEAKLRRVGEVARRVWKE"
     gene            complement(7172..>8585)
                     /locus_tag="VLF19_08725"
     CDS             complement(7172..>8585)
                     /locus_tag="VLF19_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSE93378.1"
                     /translation="SPGFMDPLCPREDDDLYQCIEWAGTQPWSNGKVGMLGISYYAIN
                     QWRVATKHPPYLAAVIPWEGFTDFYRDPSYHGGILSEFMKRWAPVQALTVQHGLGTRA
                     RKNPNTGESVAGSETLPDADLAKNRTDPHLDLLAHPLCDDWHRERSADLSKLTVPFLS
                     CANWGGQGIHPRGNFNAFVQAPAPQKWLEVHGESHWALFSAGYGLDLQKRFFDHFLRG
                     LDNGWDRTPPVTLNVRHPGERFVLRHEQEWPLARTQWTKMHLDPAAMALSRRPVTQAG
                     TVAYDALGAGVTFSWVAEQETEITGPIAAKLFVSSSTKDADLFLIVRVFEPDGKELTF
                     FGSTDPNAPIANGWLRASHRRLDPARSRPWQPYHPHDRVEPLTPGEVYECDVEIVPTC
                     IVVPAGWRVALSVRGKDYEYEGEIGEFGKTFVYGTRGTGGMTHDDPIDRPRDVFGGTV
                     TLHAGGGRESYLLLPIIPAE"
BASE COUNT         1278 a         3067 c         2971 g         1269 t
ORIGIN      
        1 ctcgagaacc tcgtgtcggt ctacacgcac cgctggctcc tgatcctcgg caccgtgtac
       61 atcgggacca tcgtgtatgc ccccgagggc atcgtgggtg cgctcagaca atggacgaaa
      121 ggagacagag catgggcaag aagctgatcg tcgcgctcct ggccgttctg gtcgccgtgg
      181 ccgtcgtgag cgtcggtcct gcacccgcgc agaaggggcc gatcaagatc ggcttcctgg
      241 ccccgatgac cgggggcgcc gcccagatcg gcaaggacat gaccaacggg ctctccatgt
      301 ggctggacga gaacaaatgg acgatcgcgg ggcgcaaggt cgaggtcatc gtggaggaca
      361 cgcaggggca gcccaacatc gcgctgacca agctgcagaa gctggtcgag agcgacaagg
      421 tgcaggtact ggcgggcggc ctctttgccc acgtcggcta cgcgctggcg cccaaggtgg
      481 accagttcaa gatcccgatg ctgtaccccg tcatggccgc cgacgacctc acgcagcgca
      541 agcccacgaa gtgggtcgtc cgcaccggct ggacgtccag ccagccctcg caccccttcg
      601 gcgagtacgc ggcgaagacg ctgggctaca agaaggtcgt gaccatcggc atcgactacg
      661 cgttcggctg ggaggtggtc gggggcttcc agaagacctt cgaggagaac ggcggccagg
      721 tcgtccagaa gctgtgggcg ccgctcaaca ccgccgactt cgcgccctat ctcagccaga
      781 tccggcgcga cgccgacgcc gtcttcgcgc tgatggtctc gatctcctcg ctgcgcttcc
      841 ccaagcagta cgagaacgcc ggtctcaagg ccaagctccc cctcatcggc gggggcacca
      901 cgttcgacga gttcgtgctg ccgtccctcg gcgacgaggc catcggcggg atcagccccc
      961 tcatctacag cgccgcgctc gacacgccgg tcaacaagcg gttcgtggcc gagtaccgca
     1021 agaagtacgg caaggtgccc tcgtactatt cggagacctg ttacacgagc ggccgctgga
     1081 tcaacgaggc tgccaaggcc gtcaacggcg acgtcgagaa ccgcgagaag ttcctggagg
     1141 ccctgcgcaa ggtcgagatc cccgacgcgc cgcgcgggcc ggtcaagctc gacgctcacg
     1201 gcaacccggt ccagaacatc tacgtccgca aggtggagaa gaaggacggc gagctgtgga
     1261 acaccgtcgt ccaggtctat ccgaacgtgt cccagttcta caagtacaag ccggaagagt
     1321 tcctcaagca gcccgtgtac gatcgaaact tccccccctg caagttctgc tgaccgtctt
     1381 gggggaaggc ccggtgcaag cggcgacgac cgcgctgacg ctctcgcgcg tctcgaaggc
     1441 gttcgggggt ctccacgcgg tcgacgccgt cagcctggag gtgcgcgccg gcgagcgccg
     1501 ggcgatcatc ggccccaacg gagccggcaa gacgacgctc ttcagcctca tcagcggcga
     1561 gcaggcgatc accggcggct cgatcgagct gttcggccgc gacatcaccc gcctggcgcc
     1621 ctaccggcga gccgcgctgg ggctcagccg cacctaccag atcacgaatc tcttccccag
     1681 gctctcggcc ctcgacaact gcctgctctc cgtgcaggcg ctgaccccgg tcaagctgca
     1741 cctgcaccgg gcactcgacc gctatcccga cctcttcgag cgtgcccgcg agatgctcgc
     1801 cgccgtcggg ctcgaggcca gggccgacca ggccgtgcgc gacctctcac acggcgagca
     1861 gcggcagctc gaggtcgcga tggcgctcgg cggctccccg aagctcctgc tcctcgacga
     1921 gccgaccgcc gggctcgcgc ccgcggaatc gcagatgatg accgcgctgc tcaagaagct
     1981 ggaccccgcg atcacgctcc tggtcatcga gcacgacatg gacgtggcct tcgcccttac
     2041 cgaccgcatc acggtgctgc actacggacg cgtcgtggcc gacggtctgg ccgagcaggt
     2101 caagtccgac ccgctcgtcc aggagatcta cctggggacc gcctgacgtg aaggccatgg
     2161 tcctgcacgg gctcggccag ccgctgcgtc tggaggacgt gccgatgccc aggatcggcc
     2221 ccaacgacgc gctgcttcgc gtgcgcgcca ccggcgtggg cctcaccgtc gtgatcatga
     2281 ccgccgtccc gggccgggtg acctccttcc cccgcatccc cggccacgag gtcgccggcg
     2341 aggtcgtcga ggtcggtggc gaggtgcgga acgtgcggcc gggcgagcgg gtcacctgcc
     2401 acttctacct gacgtgccac gcgtgcgcgt actgccgcag cgggcgcgag acgctctgca
     2461 cggcattccg cggcaacgtc ggcatggcgt gcgacggcgc ctacgccgag tacatggccg
     2521 tcccggcggc gaacctcgtt cgcatccccg acggcgtgac cgacgtcgag gcggcggtcg
     2581 ccgccgacgc catctgcacg ccgtaccacg cgtgccgcga ggaggcgcgc gtagggcccg
     2641 gcgaccgggt gctggtggtc ggcgccggcg gcggcgtggg catccacatg gtgcagatgg
     2701 cgcggctctg cggcggctgg gtcatcggcg cggacgtgga cgaggcgaag ctcgccgcgg
     2761 cgcgcgagca cggcgccgat gccgccgtcg acgcacgggg gggcgacctc gccgcgcacg
     2821 tcctgacggc gaccggcggc cgcggcgtcg acgccgtcat cgacacggtg ggctcccgcg
     2881 agacgctgga ggcgtcggtc cggtcgctgg ccatcgccgg gcggctggtc atcatcggtc
     2941 accggccgaa gcccgtcttc ggcgtggacg ccaccttcgg cgtcgacccc gggctcatgc
     3001 tcaaccgcat gctcgagatc cacggctcgc gctacgtgac gctggccgag atcgagcaga
     3061 cgctcgagct gctgcgccag cgccggctcc gggcggtggt gacgcgcacc tttcccctgg
     3121 agggtgccga ggaggcgcac ggcctgctca ggacgaacgc gctcgtggga cgcgccgccc
     3181 tcctggtggc gccctgatgc tcgacgtccg cgacatccac acctactacg gcgacagcta
     3241 cgtgctgcag ggcatctcgc tgcgcatgga gcgcgggcag gcggtggcca tcctcggccg
     3301 caacggcatg ggcaagacga cgctcatccg atccatcgtc gggttcacgc ggccacggcg
     3361 cggacagatc gtgttcaagg agcgcgacat caccggctgg ccgtccaacc ggtcggtggc
     3421 gctcggcctc ggcctggtgc cccagggacg tcgcgtgttc ccgtcgctca ccgtgctgga
     3481 gaacctcgcg gtggcctcgg cgcaacgtgg tggcggcggc tggaccgtcg acaaggtcat
     3541 ggatctcttc cctcggctcc gcgagcgggc ccagaaccgc gcgggcaagc tgtcgggcgg
     3601 cgagcagcag atgctggcca tcgcgcgggc gctgatgacg aatccggaac tccttgtcat
     3661 ggacgagccg accgagggcc tggccccgtt gctcgtgcgc gaggtgggac gcgtcatcga
     3721 gagcctcaag gcctcgggcc tgtcgatcct gctcgtggag cagaacctgc cgctggccct
     3781 ccgcgtcgcc gaccacgtcc acgtgctctc ccgggggcgc atcgtccact cgtccacggc
     3841 cgccgcgctg gccggcaacc acgagatcaa ggcccgttac ctcgggctgt gaggagagga
     3901 tcatgaagcg catcgggatc gtcggcgtcg gactcctggg ctccgcggtc gcctcgcgtc
     3961 tgctcgcggc gcgcttcgag gtcgccggct acgaccccgt gccgaagcag ctcgaggcgc
     4021 tgcgcccgcg cggcctgtgc ccggccggat ccatcggcga ggccgcggcc ggcgccgacg
     4081 ccgtgttcgt gatcttgccg tcgctcgaga ccgtggacgc cgccgtgcgc ggccccggcg
     4141 gcctcgtgga gacgctgccg cggggtggcg tgatcctgca gatgagcacg atctcgcccg
     4201 cgctcacgcg cacgctggcg gcggcctcgg ccgccaaggg cgtgggattc ctcgacacgc
     4261 cgatgagcgg cacgagcgcg atggtcgagc gcggcgattg caccgtctac gtcggcggcg
     4321 accccaccct cgccgagcgc gtcaggcccg tcttcgacgc catcgcgcgc aagaccgtgc
     4381 acgtcgggga ggccggccac gccatgctgg ccaagctggc caccaatctg ctcgtcggcc
     4441 tcaacaccgc cgccgccgcc gaggccctcg tgctcggcgc caagggcggc ctcgaccccg
     4501 ctcacctgct cgccatcctg cgcgagagcg ccgccgcctc gcggatgctc gacgtccggg
     4561 gcccgctcat ggtcgagcgg aagttcgacg cgcagatgaa gtgcgatctg ttcctgaagg
     4621 acttcaagct catgctggac gagggcatgc gactcggggt ggccctgccg ctcaccagcc
     4681 tcacccagca gctcacgacc gccaccgtcg ccgccgggcg cggcggcgag gacctcgcgg
     4741 ccctcatcaa gacgatcgag gacctggccg ggatgggaga ctgagaatgc gcttcgggac
     4801 cttcttcttc ttccaggcgc cgcccggcgt ccggcacgac gagatcatcc acaacgagct
     4861 cgagcagatg gagtggacgg aagagctggg cttcgacgag gtgtggctca ccgagcacca
     4921 cttcatcgac tacgggctgg ccgtggaccc ggcctcgctc gcggccgccg cggcgtcccg
     4981 cacgcgccgc gtgcgcatcg gcctggccgc ggcgatcctg cccttccacc atccgatccg
     5041 cctcgccgag cagatggcgt tgatcgacat catcagcaag ggccgcctcg acgtcggcgt
     5101 gggccgtggc aaccgccccg cggagttccg cggctatcac gtgccccagc aggagagccg
     5161 cgaccggttc gacgagaccg tggagctgat ggtgcgcgcg tggacggagg agcacgtcag
     5221 tcaccagggc cggttcttca cggtgcccga ggcgcgcgtc atccccaaac ccgtgcagcg
     5281 cccgcacccg ccgctctacc aggtgtgcgt caccaaggac ggcatcgaga acacggcgct
     5341 gcgcggctgg cccatgctca actcggtgct cttcggcccc gtggaccagc tcgtcggcaa
     5401 tcgcgacatc tacctggaca cgctccgcaa ggccgggcgc agcgaggacg aagtgaaggc
     5461 cctcctccgc cgctggggcg tgagccgcca gatctacgtc gccgactccg acgccagggc
     5521 cctggaggac gccaggccgt acgagatgtg gtaccaggac tcgttccgcc gcttcgtcgt
     5581 tcccgaccgc atcgaggacg cgcacccgac gctgcagccg ggcttccgcg cgatggccga
     5641 tcggctctcg aaggtcacgt gggagggact cgtcgccgag acgctggcct tcggctcccc
     5701 cgacaccgtg gcccgtcaca tcgagcacat gcgctcgatc ggcgtggggc aggtcctctg
     5761 ctggatgaac ttcggcggcc tgcctcaggc caaggtgcgc cgctcgatgg agctgttcgc
     5821 ccgcgagatc atgccccgct tcaggtagga gggtgtcggg ttaccgaggc gcgtcacgac
     5881 tcggccgggt gtgccgaacg ctgggggttg gggggccatg tcgggggccc ccatgtcatt
     5941 actccttcca gacgcgccgg gcgacctcgc cgacccgccg cagcttggcc tcctgctccg
     6001 cgggcgtgag cggattaccg gcctccccgg aggcgaagcc gcactgcggg ctcaccgcca
     6061 gacgctccag cggcacgtgg cgggtcgcct cgcgaatgcg ggactccacg gccgcgccgt
     6121 cctcgagggc gccgcgcttg gtcgtgagca gacccagcac cacctccgtc cccggacgga
     6181 cgtgcacgag cggtccgaag tctccggccc gcggcgtgtc gtactccagc agcagccggt
     6241 cgtagcgcgt gagacgggga aacacctggc cggcgatgcg ctcgtagccg ccgttggcca
     6301 gccagcggcc gccgggcgcg ttgccccggc agatgtggat cgcgcgcgtg ataccgcgca
     6361 cgccctcgaa cacggtgtcg tcgatctcgg cgtcggcgac gagctctctg tccatgtcgt
     6421 gaccccacga ctcgaaggcg gcacggttgt cgcggtcgac gtgccactgc gcgtagttgg
     6481 gcgcgtccat ctggacgtag tcgccgccca gggcggcgat cttcttcacg acctcgtcgc
     6541 gcagatacgc ggccaccgcg cgcaggaagt cgtccgccgt cggataggcc gcccgcgagt
     6601 gctccgggtg ccagaagatg cggtgccagc tcggggcggg aatcgtgaac ttcgtccggg
     6661 ctcgggcgtt ggccttgagg aacgtgtact cgtcggcgac gatgtcgcgc acgacccgga
     6721 gcggctgcgt cacgaacggc ctcgtgtcac gggtccatac ctgggcgggc cgcccgtcgg
     6781 gctccttcca gagcgaccac cacccggggc cggccggcag gaactcgtag cccgccagcg
     6841 gcgcgtgggc agcgccctcc tcgcggagcg ggatcgtcac cacccacgag ttgcggcgga
     6901 gctcgccgtc ggtgacgacg tcgatgcccg ccgactcctg cagcgcgatg gcctcccgca
     6961 ccgcgcggtc ctccactgcc gcgagccggc cagcgtcgag ccgtccttcc cgcaccgcct
     7021 gccgggcgtc cttcaggtac tggggccgga gcaggcttcc gaccacgtcc gcgcgcgcgg
     7081 ggtcgagccg ggtcatgact tcgtcagcct cccttcctcg cagggcgccg cccacggatc
     7141 cggatcgatc gagcgcatca cggaaccggc cctattccgc cgggatgatc ggcaggagca
     7201 ggtacgactc gcgcccgccg cccgcgtgca gcgtcaccgt cccgccgaag acgtcacgcg
     7261 gtcggtcgat cggatcgtcg tgcgtcatgc cgcccgtacc ccgcgtgcca tagacgaacg
     7321 tcttgccgaa ctcgccgatc tcgccctcgt actcgtagtc cttgccgcgg accgagagcg
     7381 ccacccgcca gcccgccggc acgacgatgc acgtcgggac gatctcgacg tcgcactcgt
     7441 agacctcgcc gggcgtgagc ggctcgacgc ggtcgtgcgg atggtacggc tgccacggcc
     7501 ggcttcgcgc gggatcgagc cgccggtgcg aggcgcggag ccacccgttg gcgatcggcg
     7561 cgttggggtc ggtggagccg aagaacgtca gctccttacc gtccggctcg aagacgcgga
     7621 cgatcaggaa caaatcggcg tccttcgtgg acgacgagac gaagagcttc gccgcgatgg
     7681 ggccggtgat ctcggtctcc tgctcggcca cccacgagaa cgtcacgccg gcgccgagcg
     7741 cgtcgtacgc gaccgtcccc gcctgcgtca cgggccggcg gctcagcgcc atcgccgcgg
     7801 ggtccaggtg catcttcgtc cactgcgtgc gggccagtgg ccactcctgc tcgtggcgga
     7861 gcacgaatcg ctcgccgggg tggcggacgt tgagcgtcac cggcggcgtc cggtcccagc
     7921 cgttgtcgag ccccctgagg aagtggtcga agaagcgctt ctgcagatcc agaccgtagc
     7981 ccgcggagaa gagggcccag tgggactcgc cgtgcacctc gagccacttc tgcggggccg
     8041 gcgcctgcac gaaggcgttg aagttgcccc gcgggtggat gccctggcct ccccagttgg
     8101 cgcacgacag gaagggcacg gtgagcttcg agagatcggc cgagcgctcc cggtgccaat
     8161 cgtcgcagag cggatgggcg aggagatcca ggtgcggatc ggtgcggttc ttggcgaggt
     8221 cggcgtcggg cagcgtctcc gacccggcga ccgactcgcc ggtgttgggg ttcttccgcg
     8281 cgcgcgtgcc caggccgtgc tgcacggtga gcgcctgcac cggggcccag cgcttcatga
     8341 actcgctgag gatgccgccg tggtagctgg gatcgcggta gaagtcggtg aacccctccc
     8401 acgggatgac ggccgcgagg tacggcgggt gtttcgtggc cacgcgccac tggttgatcg
     8461 cgtagtagga gatgccgagc atgccgacct tgccgttgct ccacggctgg gtgcccgccc
     8521 actcgatgca ctgatagagg tcgtcgtcct cccgcgggca gagcgggtcc atgaagcccg
     8581 gcgac
//
LOCUS       DATIAZ010000245         4236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_856723, whole genome shotgun sequence.
ACCESSION   DATIAZ010000245 DATIAZ010000000
VERSION     DATIAZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856723"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="pdhA"
                     /locus_tag="VLF19_08730"
     CDS             complement(<1..904)
                     /gene="pdhA"
                     /locus_tag="VLF19_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HSE93379.1"
                     /translation="MPRKILEPRFSVEHLSILDSDGNLDAALEPDLPPDQLKAIYRAM
                     LLGRRLDERMVRLQRQGRIGTFAPIKGQEASQIGSVWTLRPTDWMVPSFRETAAMLRR
                     GWPIEKLLLFFAGYLEGGRPAPEQRDLPVTIPVATQLPHAVGLAYAAQYRGDDVVVMA
                     YFGDGATSEGDFHEALNFAGVWHVPVVFVCQNNQWAISVPLKKQTHSRTLAQKALAYG
                     LPGIQADGNDVLAVYAASREAVDRARAGDGPTLIECVTYRLGMHTTADDPTKYRTEEE
                     VRAWERKDPLTRFAAYLGKRNLLED"
     gene            complement(961..1758)
                     /locus_tag="VLF19_08735"
     CDS             complement(961..1758)
                     /locus_tag="VLF19_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSE93380.1"
                     /translation="MDYADYEHLAFERRPHGVLLITINRPEVLNAANDRLHWELTQIW
                     LTIDRDDDTRVAVITGAGRAFSAGGDLEMVEANATDPKRLANTVREASDIVYNMINLD
                     KPVVSAINGVAVGAGLVVALMADVSVIAEDVRFTDGHTRLGVVAGDHAAVIWPLLCGM
                     AKAKYYLLTSDFIDGREAERIGLVSLCVPRAELLPRAFDVAGKLAQGSQQAIRWTKRS
                     LNNWLRMAGPIFDQSIALEMLTFMGEDVREGLRAIREKRPPRFPSAR"
     gene            complement(1814..1921)
                     /locus_tag="VLF19_08740"
                     /pseudo
     CDS             complement(1814..1921)
                     /locus_tag="VLF19_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
     gene            complement(1980..2870)
                     /locus_tag="VLF19_08745"
     CDS             complement(1980..2870)
                     /locus_tag="VLF19_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSE93381.1"
                     /translation="MPPHAIGSGTISFGLVSIPVKLYTATSGGTVSFNLLHAKCGSRI
                     RQQTFCPTCNAVVDRNELVRGYEFAKDQYVRVGDDELKALEGEASKTIDITEFVPLEK
                     VDPIYFERTYYLGPDKGGEKAYRLLANAMGKSGRVALAKFVMRGKESLVLIRSAQDGL
                     MLHTMYFTDEVRSFGEIDKGKTAPVRPNEMELAQQLIDGLTNDEFHPEQYQDEYRGRV
                     LDLVNRKVEGREVTVAAPPAPKAQVIDLMEALRESLAKRVPAAEAREAAAARKPPSKA
                     VPKRAEPARAAPRKAQGGRK"
     gene            3000..>4236
                     /gene="ligD"
                     /locus_tag="VLF19_08750"
     CDS             3000..>4236
                     /gene="ligD"
                     /locus_tag="VLF19_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HSE93382.1"
                     /translation="MDGTLASAADPARAADPAALRARLARVRAPRGEIPASQSPMLAT
                     LVDRVPPGPGWLFEVKYDGVRVIARREGTRVTLAGRNGQDFTPRYPELIEALRALTVE
                     RFVLDGEVVAVDERGRPSFQRLQNRMHLTRPVDVARARETVPVSAVFFDALALDGHDL
                     RALPLAERKACLALVLPSRGVVRYGGHVAERGDAFLAAAAEQQLEGIVAKKVDSAYGA
                     GRSRDWLKIKCQLRQEFVIGGWTDPQGSRPYFGALHLGLYEDGRLVYVAKVGTGFDGK
                     TLTLVWRKLGALARATSPFATVSPRGRGHHWVEPRLVCEVRFTEWTEDGGIRHPAFLG
                     LRDDKRPEDCRRETPLHVIGAGASGTTATPEAPAPARARPSAGGGRARPKARVARAQA
                     RAARPPAGTPTKRARAAGPA"
BASE COUNT          562 a         1503 c         1547 g          624 t
ORIGIN      
        1 cgtcctcgag gaggttcctc ttgcccaggt acgcggcgaa gcgcgtgagc gggtccttgc
       61 gctcccacgc acgcacttcc tcctcggtgc gatacttggt tgggtcgtcg gcggtggtgt
      121 gcatgcccag ccggtacgtc acgcactcga tcagcgtggg gccgtcgccg gcgcgcgccc
      181 ggtcgaccgc ttcccggctc gccgcgtaca ccgcgagcac gtcgttgccg tccgcctgga
      241 tgccggggag gccgtaggcg agggccttct gcgcgagcgt ccgggagtgc gtctgcttct
      301 tgagcggcac ggaaatggcc cactggttgt tctggcagac gaacaccacg ggtacgtgcc
      361 agacgccggc gaagttgagc gcctcgtgga agtctccctc ggaggtcgcg ccgtcgccga
      421 agtacgccat caccaccaca tcgtcgccgc gatactgggc ggcgtaggcc agaccgacgg
      481 cgtgcggcag ctgggtcgcc accggaatcg tcaccggcag gtcccgctgc tcgggcgccg
      541 gccggccgcc ctcgaggtag cccgcgaaga agagcagcag cttctcgatg ggccagccgc
      601 gccggagcat ggccgccgtc tcgcggaacg aggggaccat ccagtcggtc gggcgcagcg
      661 tccagacgct cccgatctgc gaggcctcct ggcccttgat cggcgcgaag gtgccgatgc
      721 ggccctgccg ctgcagccgc accatgcgct cgtcgagccg gcggccgagc agcatcgcgc
      781 ggtagatggc cttcagctgg tcgggaggca ggtcgggctc gagcgcggcg tccagattgc
      841 cgtcgctgtc gaggatggac aggtgctcga cggagaaccg aggctcgagg atctttctgg
      901 gcatgggccc ggctcgtgac cagtgtaccg gaaaacccga ccgatgctct ccggcttccg
      961 tcagcgcgcc gacgggaagc gcggagggcg cttctcgcgg atggcgcgga ggccctcgcg
     1021 gacgtcctcg cccatgaagg tgagcatctc cagcgcgatc gactggtcga agatcggccc
     1081 ggccatgcgc agccagttgt tgagcgagcg cttggtccag cggatcgcct gctggctgcc
     1141 ctgcgcgagc ttcccggcca cgtcgaaggc ccgcggcaac agctccgcgc gcggcacgca
     1201 gaggctcacg aggccgatgc gctcggcctc ccggccgtcg atgaagtcgg aggtcaacag
     1261 gtagtacttc gccttggcca tgccgcacag gagcggccag atgacggccg cgtggtcgcc
     1321 ggcgacgacc cccaggcgcg tgtggccgtc ggtgaagcgt acgtcctcgg cgatcacgct
     1381 cacgtcggcc atcagcgcca ccacgagtcc ggcgccgacg gccacgccgt tgatggccga
     1441 gacgaccggc ttgtcgaggt tgatcatgtt gtagacgatg tcggaggcct cgcgcacggt
     1501 gttggccagg cgcttgggat ccgtggcgtt cgcctccacc atctcgaggt cgccgcccgc
     1561 cgagaaggcg cggcccgccc ccgtgatcac ggcgacgcgg gtgtcatcgt cgcggtcgat
     1621 cgtgagccag atctgggtca gctcccagtg cagccggtcg ttggcggcgt tgagcacctc
     1681 ggggcggttg atcgtgatca gcagcacgcc gtgcggacgg cgctcgaagg cgaggtgctc
     1741 gtagtccgcg tagtcgatca cgcccgggtg ctcaggtctg ggccgcgccg ttggcgccga
     1801 tgatcggcca cgtgttgacg acggcggcag caccgcgccc ggcgagctcg agcacgatcg
     1861 cccggccgat gttgcggccg gcgccggtgg cgagcgcgac gcggccctcg agagtggaca
     1921 tggcgtcgcc agttgaccat caaccgcaag cccccgcaag cgcggcggcg ccccgggcgc
     1981 tacttcctgc cgccctgggc cttcctcggc gcggcgcgcg ccggctccgc gcgcttgggg
     2041 acggccttgg agggcggctt gcgggcggcg gcagcctcgc gcgcctcggc ggcagggacg
     2101 cgcttggcca ggctctcgcg gagcgcctcc atgaggtcga tgacctgcgc cttcggcgcc
     2161 ggcggcgccg ccacggtgac ctcgcggccc tcgaccttcc ggttgacgag gtcgaggacg
     2221 cgaccccggt actcgtcctg gtactgctcg ggatggaact cgtcgttggt gagaccgtcg
     2281 atgagctgct gggccagctc catctcgttg ggtcggaccg gcgccgtctt tcccttgtcg
     2341 atctccccga agctgcggac ctcgtcggtg aagtacatcg tgtggagcat gagcccgtcc
     2401 tgcgccgagc ggatcagcac gaggctctcc ttgccgcgca tcacgaactt ggcgagcgcg
     2461 acgcgcccgg acttccccat cgcgttggcc agcagccggt aggccttctc cccgcccttg
     2521 tcggggccga gatagtaggt ccgctcgaag tagatcggat cgaccttctc gagcggcacg
     2581 aactcggtga tgtcgatcgt cttcgacgcc tcgccctcga gcgctttcag ctcgtcgtcg
     2641 cccacgcgca cgtactggtc cttggcgaac tcgtagccgc gcaccagctc gttgcggtcg
     2701 acgaccgcgt tgcacgtcgg gcagaacgtc tgctggcgga tccgcgagcc acacttggcg
     2761 tggagcaggt tgaacgacac cgtgcccccc gacgtcgccg tgtacagctt caccggaatc
     2821 gacacgagtc cgaaggagat ggtgcccgag ccgatcgcgt ggggcggcat aatgcggagg
     2881 gtagcacgaa gccgcgcggg ccaggaacgt ccgtgccgcg gcgcggcggg gtgttccacc
     2941 aggcgcggcg gcgtgctccg cccgcgcggc gaggcttcca ccatgagttg cggtcggcga
     3001 tggatggcac actagcgagc gcggccgacc ccgcccgggc cgcggatccg gcagcgctgc
     3061 gcgcccggct ggcccgggtg cgcgcgccgc gcggggagat ccccgcctcg cagtcgccga
     3121 tgctcgcgac gctggtcgac cgcgtgcccc ccggtcccgg ctggctgttc gaggtgaagt
     3181 acgacggcgt gcgcgtgatc gcccgccggg agggcacgcg cgtgacgctg gccggccgga
     3241 acggccagga cttcacgccc cgttacccgg agctgatcga ggcgctgcgc gcgctgacgg
     3301 tcgagcgctt cgtcctcgac ggcgaggtcg tggcggtcga cgagcgcggg cgcccctcct
     3361 tccagcgcct gcagaaccgc atgcatctca cccgccccgt cgacgtcgcg cgggcccgcg
     3421 agaccgtgcc cgtgagcgcg gtcttcttcg acgccctcgc cctcgacggt cacgacctgc
     3481 gcgcgctgcc gctcgccgag cgcaaggcat gcctggccct cgtgctcccg tcgcgcggcg
     3541 tcgtgcgcta cggcggccac gtggccgagc gcggcgacgc gttcctcgcg gcggcagccg
     3601 agcagcagct cgagggcatc gtcgccaaga aggtggacag cgcgtacggg gccggacgct
     3661 cgcgcgactg gctcaagatc aagtgccagc tccgccagga attcgtgatc ggcggctgga
     3721 cggacccgca gggctcgcgc ccgtacttcg gcgcgctgca cctcggcctc tacgaggacg
     3781 gccgcctcgt gtacgtcgcg aaggtcggca ccgggttcga cgggaagacg ctgacgctcg
     3841 tgtggcggaa gctgggggcg ctggcgcgcg cgacctcgcc gttcgcgacc gtctcgccgc
     3901 gcggccgcgg tcatcactgg gtcgagccgc gcctcgtgtg cgaggtgcgc ttcaccgagt
     3961 ggacggagga cggcggcatc cgtcaccccg cgttcctcgg gctccgcgac gacaagcggc
     4021 ccgaggactg ccggcgggaa acacccctgc acgtgatcgg cgccggcgcg tccgggacca
     4081 cggcgacgcc cgaagcgccg gcgccggccc gcgcgaggcc gtcggcgggc ggcgggaggg
     4141 cgcggccgaa ggcacgggtc gcgcgggccc aggcacgtgc ggcccgcccg cccgccggca
     4201 cgcccacgaa gcgcgcgcgc gccgcgggac cggcgg
//
LOCUS       DATIAZ010000246         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1250617, whole genome shotgun sequence.
ACCESSION   DATIAZ010000246 DATIAZ010000000
VERSION     DATIAZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250617"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1767)
                     /locus_tag="VLF19_08755"
     CDS             complement(82..1767)
                     /locus_tag="VLF19_08755"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HSE93383.1"
                     /translation="MTEGLARGLTNYGDRDFALYLRRSFARSMGYSTAALARPIVGIA
                     DSASGFNNCHRHLPELIEAVKRGVLAAGGLPIAFPTISLGEVFLDPTSMLYRNLMAMD
                     VEEMVRAQPMDAVVLVGGCDKTVPAQLMGAASAGVPAIQLVAGPMMTGRYETERLGAC
                     TDCRRFWAQHRAGKITKDQIEAVEGRLATTTGTCAVMGTASTMACLSEALGMALPGTA
                     AIPAVHADRLRAAEATGQTAVALAHSRLTPDRILTREAFENALRVLLAIGGSTNAIVH
                     LTAVAGRVGVTIPLDLVNRLSDDTPVLVDLKPSGQHYMEDFFAAGGVGAVLRELAPKL
                     HARCLTVTGETLGERLGAENGWVDRGVIRSTAEPFLPVGGLVALFGTLAPRGAILKRS
                     AADARLFESEGRAVVFTSLDDLARRIDDPGLDVTPADFLVLQNAGPIGAPGMPEAGYL
                     PIPGKLARAGVTDMVRISDARMSGTAYGTIVLHVAPESAAGGPLALVRTGDRIRLSVR
                     ARRIDLFVDESELARRRAQAPTSTDRAPRGYRRLYLDHVLQADEGCDFDFLRG"
     gene            complement(1764..2441)
                     /locus_tag="VLF19_08760"
     CDS             complement(1764..2441)
                     /locus_tag="VLF19_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93384.1"
                     /translation="MRWRTRGFLAAGAVATLATGIFTGVRRAERRRAQALAESTARLG
                     LVFARRDDVAVSPVVLRLPLFTRSWLKKGRVGVVRNVAHGVVGDRWVLTFDYDFYPGG
                     PRPSFTVVAIPWRGVMLPELQLQARHGMPATPRLRAPAYVLDGASPRRAYVLRVGDEA
                     AAGRLRDSLLVAFLREQPAPLCVEAAGEWVIVYRPRIRAAELDDLVGRALDVGRLAAG
                     TFVSHAP"
     gene            complement(2438..2620)
                     /locus_tag="VLF19_08765"
                     /pseudo
     CDS             complement(2438..2620)
                     /locus_tag="VLF19_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2886..>3104)
                     /locus_tag="VLF19_08770"
     CDS             complement(2886..>3104)
                     /locus_tag="VLF19_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HSE93385.1"
                     /translation="RERGLQQAFFVGLATDFCVAWSALDARRAGFAALVIEDATRGID
                     AGGSLGKAWTDMTGAGVKRIASSDIATP"
BASE COUNT          439 a         1117 c         1156 g          392 t
ORIGIN      
        1 taccagcgag ggtgtcgtct ccatcggcac gccgcgcctg gtccgcggag ggctccgcct
       61 gacgttctga gggagtccgg cctagccgcg caggaagtcg aagtcgcagc cctcgtccgc
      121 ctgcagcacg tggtcgaggt agagacgccg atagccacgg ggcgcgcggt cggtgctcgt
      181 aggtgcctga gcgcggcgac gcgcgagctc cgactcgtcg acgaacaggt cgatccgacg
      241 cgcgcgcacg ctgagccgga tgcgatcgcc ggtgcggacc agggcgagcg ggccgccggc
      301 ggcggactcc ggcgccacgt gcagcacgat ggtgccgtag gccgtgccgc tcatccgtgc
      361 gtcggagatg cgcaccatgt cggtgacgcc ggcgcgcgcg agcttgccgg ggatcgggag
      421 ataaccggcc tcgggcatcc ccggggcgcc gatcggcccc gcgttctgga gcacgagaaa
      481 gtccgccggc gtcacgtcga gcccggggtc gtcgattcgg cgtgcgaggt cgtcgagcga
      541 cgtgaagacg acggcgcggc cctcgctctc gaagagccgc gcgtcggccg ccgaacgctt
      601 gaggatcgcc ccgcgcgggg cgagcgtgcc gaacagggcg acgaggccgc cgacgggcag
      661 gaagggctcc gccgtgctcc ggatcacacc gcgatccacc cagccgttct cggcgccgag
      721 ccgctcgccg agcgtctcgc cggtcaccgt gaggcaacgc gcgtggagct tcggcgcgag
      781 ctcccgcagg acggctccca cgccgccggc ggcgaagaag tcctccatgt agtgctggcc
      841 cgagggcttc aggtccacga gcaccggtgt gtcgtcgctc agccggttga cgagatcgag
      901 cgggatcgtc acgccgacgc gtcctgcgac ggccgtcaga tgcacgatgg cattcgtgga
      961 gccgccgatg gcgagcagca cgcgcagcgc gttctcgaag gcctcgcgcg tgaggatgcg
     1021 gtcgggcgtc aggcgcgagt gcgccagcgc caccgcggtc tgccccgtcg cctcggccgc
     1081 gcgcaggcgg tcggcgtgga cggcgggaat cgcggcggtg ccgggcagcg ccatgccgag
     1141 cgcctcgctg agacaggcca tcgtgctggc ggtgcccatg acggcgcacg ttcccgtggt
     1201 cgtcgccagc cgcccctcga ccgcctcgat ctggtccttg gtgatcttac ccgcgcgatg
     1261 ctgggcccag aagcgccggc agtccgtgca ggcgcccagc cgctcggtct cgtaccgtcc
     1321 cgtcatcatc gggccggcga cgagctggat ggcgggcaca ccggccgagg cggcgcccat
     1381 gagctgcgcg ggcacggtct tgtcgcagcc gcccaccagc acgacggcgt ccatgggctg
     1441 ggcgcggacc atctcctcga cgtccatcgc catgaggttg cgatacagca tgctcgtcgg
     1501 atcgaggaag acctcgccca gcgagatggt cgggaacgcg atcggcagcc cgccggccgc
     1561 cagcacgccg cgcttcaccg cctcgatcag ctcggggaga tgccggtggc agttgttgaa
     1621 gccgctggcg gagtccgcga tgccgacgat cgggcgcgcc agcgcggcgg tcgagtagcc
     1681 catggagcgc gcgaacgagc gacgcaggta gagggcgaag tcgcggtcgc cgtaattcgt
     1741 gaggccacgc gccagccctt cggtcatgga gcgtggctca cgaacgtccc ggccgcgagc
     1801 cggccgacgt ccagggcgcg gccgacgaga tcgtcgagct cggcggcgcg aatcctgggc
     1861 cgatacacga tgacccactc gccggcggcc tcgacgcaga gcggcgctgg ctgctcgcgg
     1921 aggaaggcga cgagcaggga gtcccggagg cgccccgcgg ccgcctcgtc acctaccctc
     1981 agaacgtacg cacgccgggg cgacgcgccg tccagcacgt acgccggcgc tctcagccgc
     2041 ggcgtcgccg gcatgccgtg gcgggcctgc agctggagct cgggaagcat cacgccgcgc
     2101 cacgggatgg cgacgaccgt gaacgacggc cgaggtccgc ccgggtagaa atcgtagtcg
     2161 aaggtcagca cccatcgatc gcccacgacc ccgtgcgcca cgttgcgcac gacaccgact
     2221 ctccccttct tgagccatga ccgcgtgaac agcggaagcc gcaggacgac cgggctgacc
     2281 gccacgtcgt cccgcctggc gaagacgagg ccgagccggg cggtgctctc cgcgagcgcc
     2341 tgcgcgcgac gacgctcggc gcgccggacc ccggtgaaga tgccggtggc gagcgtcgcc
     2401 acggcgccgg cggcgaggaa acctcgggtg cgccacctca cgcgtgcccc cgcgcgaggc
     2461 gctcggtcgc gatcggcagc gcggtgatcg tcaccccgag cgggcggacg gcgtcggcga
     2521 cggcgttggc gatggcggcc ggcgcgccaa tggcgccgcc ctcacccttg cccttcacgc
     2581 cgccgggcac ctgtggcgcg ggcgtctcca ggtggccgat gctcggctcg ggcgcgtcgg
     2641 cggacccgtg ctcgtacacc ggcgtgcggt gggggccggg cgggatcgag gcgctgccga
     2701 gaggctccag ggccgccgtc agcggataga tgtagcggcc gcggagcagg cgcgggtcct
     2761 ccaggcgctt gatccgcccg ccggtgtagc ggatcgtcac ggcctcaggc gcgcacgcgc
     2821 ggcagcacct cgcccgcgat cagctcgagg ggctcgcgcc cccagtcgag gtcgatcgac
     2881 gctgctcacg gggtcgcgat gtccgacgag gcgatacgct tgacacccgc cccggtcatg
     2941 tccgtccacg ccttgcccag cgagccgccg gcgtcgattc cccgcgtggc gtcctcgatc
     3001 acgagcgcgg cgaatccggc gcgccgggcg tcgagcgccg accacgccac gcagaagtcg
     3061 gtggcgaggc cgacgaagaa ggcctgctgc aggccgcgct ccct
//
LOCUS       DATIAZ010000247         8955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1513169, whole genome shotgun sequence.
ACCESSION   DATIAZ010000247 DATIAZ010000000
VERSION     DATIAZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513169"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..890)
                     /locus_tag="VLF19_08775"
     CDS             complement(387..890)
                     /locus_tag="VLF19_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSE93386.1"
                     /translation="MELVGRMMDGTIPPPGGGPKRGFFGWRVSQDAKVQQLQRVPLLA
                     ECSRRQLRAVAQIAEIRELPAGGVVTRAGDPGDEFFVIIDGRARAEVSSRKRPRLGPG
                     DFFGEMSLLDGERRSATVVAETPVRLLVIPRREFSRVLAEVPDLTRNLLAVLSRRLRQ
                     AEQALNA"
     gene            complement(893..1906)
                     /locus_tag="VLF19_08780"
     CDS             complement(893..1906)
                     /locus_tag="VLF19_08780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE93387.1"
                     /translation="MSFTIVDADGHVTESQEQLLKYVDEPYRRRPTGFLWVPQDGWDR
                     RLLGTLGDWAGTADEWLQALDRGGMESVVLYPTLGLFMSFLKDREWAVALCRAYNTFL
                     HEEFVRVSPRLKGVALLPVQDPDAAAKELRRAVRECGLVGAMLAADGTHVLGDERFAP
                     IYEEAQRLNVMLGIHASGSHLGGGGVDLFPRFIQAHTCSHAFGQMRQLTSIVFEGIPE
                     RFPDLRLAFLEAGCGWAPYWMERMDDEYAKRASEAPALKKKPSEYIRSGRIYFSCEAD
                     EWLLPQAVKLVGENQIVYASDFPHWDHSYPQSVEEIRARGDLSDRQKQKIFADNARRL
                     YGL"
     gene            complement(1926..3638)
                     /locus_tag="VLF19_08785"
     CDS             complement(1926..3638)
                     /locus_tag="VLF19_08785"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HSE93388.1"
                     /translation="MRKRASVESAAEAYLELLAARHVEYFFGNAGTDFAPIIEAYARR
                     EAQGQVLPRPMTVPHEIPAVAMAHGYAMVTGRAQVVMVHVTVGTANALGGLINAARAR
                     APILMTAGRTPITEGGLPGSRNRHIHWAQESFDQGAMVREYVKWDYELRLGAQVETVV
                     DRALAIAQSTPAGPVYLTLPREVLSERMTEIEYFDPPRMRPAAAQVTDPAAVEDAARV
                     LAAARNPIAIVKSSGRDPGAVAPLVALAEALGMPVFEHASTHLCFPQTHALHAGGDPA
                     PYFGEADAILVVEADAPWYPALRSPRPETPVIQVGEDPLYSRYPIRGFAADHALAGAP
                     RLTLVALAEAVRRAGLDGRSVAARRGRWEGEHRRMTEAAAARAQAVRNDRPIDMAWLS
                     RAVGDVLDDRTIVVNEYDLDLAHTRLSVPGSYFSQSPASGLGWGLGAALGAKLAAPDR
                     TVVSCVGDGAYIFGVPTAAHWAARAHNLPVLFVVFNNRVWNAVRRSVQSHAPEGWSMR
                     TKTIPLSDLDPAPDYEKICEACGGWGERVEDPADLPNALARALRVVREDKRQALLNVI
                     CKKP"
     gene            3916..4842
                     /locus_tag="VLF19_08790"
     CDS             3916..4842
                     /locus_tag="VLF19_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93389.1"
                     /translation="MFTYAVRRVLWLVPTLLAMALVTFLVMHATPGSPLDPVAEGANP
                     LSPEAQKNLAAHYGLDRPLWKQFAIFVGNAVQGEFGNSFVYKTRPVREILAETLPVSL
                     LLGTMALVLAVAAGLTLGILAAIHQNRAWDYVSVSLATFGVAVPNFVLAVFLIILFSF
                     VVPLFPTGGWDSPRNWVLPTITLALGPMGIIARFTRASMLEVIRADFTLTARAKGLGE
                     APVIFKHVLKNAFIPIVTLLGPMFAAVGTGSFFVESIFRVPGMGRFFVLSMTGRDYPM
                     IMAVVLTYGAFLAVMNLVVDLLYGALDPRIRY"
     gene            4811..5728
                     /locus_tag="VLF19_08795"
     CDS             4811..5728
                     /locus_tag="VLF19_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93390.1"
                     /translation="MALSTPASATSDGVVAAAAPVVAPRGSSLGRDAFRRLRRNRLAM
                     AGAVVVVLLCLVAVFADVLAPYAYTKTNFGRLNEAPSRDHPFGTDQLGRDLLSRMVYG
                     ARISMLVGLGAQVIVVLIGVPIGALSGYLGGRVDLLLTRFVDVMYAFPRLLFVILLMS
                     MLGAGLTNIFIAIGLTGWVSIARQTRAQVLALKEKEFAEGARALGAGWARVLGRHVMP
                     NALTPIVVSITFGIPEAIFTEAALSFIGVGINPPTPSWGQMVGEGQQYLRSAWHLCVI
                     PSIAIATTMLSFTFFGDGVRDALDPKLKQ"
     gene            5807..7543
                     /locus_tag="VLF19_08800"
     CDS             5807..7543
                     /locus_tag="VLF19_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HSE93391.1"
                     /translation="MRIPAISDQQLDLLATRLAAIGVGRRDFLVVAAGLATMGAAGFT
                     ARPASAAPKPAPGEKLAKDQHLRFGGGSWYEQDPASHDFNKDLYCNGTPQLWAGLMKF
                     NVDFQAMPYVASKVTPNQDGSAWTFTIRKDAKWSDGSPCTARDFEWSFKRQLDPATAA
                     PYASFLYDIKNGEAFNKKQVTDPGQVGVRAKDDWTLEVTLEGPRGYFPVLSAYQAAYP
                     AHRRSVEKFGDKWTEAANIVCNGPFVLETWEHNKVIVLKKNKHFFDAKDVTLEKVTIP
                     IIPIASGVLPYENNEIDLSRLQTADLRRFQSSPATQKQVFRFPYPGTWYLLPQVTMPP
                     FDNVKVRRAVAHAIDRENVVKVSQGFAIPAHSMIPPGFPGALDDARTRSLQKFDPKLA
                     MAQLKGTPFEGGRNWPKITLTLRDEALGSKPLAEAVQAVLLEHLNMKTDLEVLEPRVF
                     RERLWKNDLQFVWIRWFMDYPDPHNEYFDTFYGKKTTGKRQAWTNDAFDKELEAGRDT
                     RDPKKRLEHYARAEQIMQTDVGYVPVAWVVRYFVAKPYVRGIEKNRQGEYVVDGNIYV
                     DMLRHIYMVEKS"
     gene            complement(7540..8565)
                     /locus_tag="VLF19_08805"
     CDS             complement(7540..8565)
                     /locus_tag="VLF19_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE93392.1"
                     /translation="MSTNVATKIDVFPHVLPRRYFDRMLAVAPPGMALQKRMSGIPVL
                     VDVDLRLRMMDRHEGYVQVLTLANPPIEVVADPARSPDLARLANDEMAALVARYPDRF
                     PGFVASLPMNAPDAAMKEIERAVGDLGATGVQVFTNVNGRPLDRPEHLAVLERMAALR
                     LPVWMHPARPATVADYAGEPRSRYDMWWAFGWPYETSVAMGRLVFSGIFDRHPDLAVI
                     THHLGAMIPFCAGRVGGGLDQLGSRSDDPDDLAALGRLARRPIDYFRMFYGDTALFGA
                     WHAMESGLEFFGADHVLFGTDFPFDPERGPGFIRDTIAAMEKMRASADDKTKIYEGNA
                     RRMLKLR"
BASE COUNT         1334 a         3177 c         3085 g         1359 t
ORIGIN      
        1 gagggcggtg atctcgaagg cggggtcgat ctccctggac acgacgacca gctcatcggg
       61 ccgcctgcgc ttgacgagat cgaggtagcc gcggaggtcc tggggcacgg ccgcgattat
      121 actaggctcc ccacggtgcc ggacgccgag ggaacgactt gttgccggcg acgcgggcag
      181 ggctgcgggc gccaccccgg gggcgacccg cgacggtgcg cccggggacg agaaggacgt
      241 agtcaccgat tccccgagcc agatggaagc cacgtcggca gcagcccgcg cctccggcag
      301 tgcgtgtcga tgcgcctggg cccaggggtg cctgcgccgt caagcgcgcg acgaccgtcg
      361 gcgggccgtc gggtccgccg acggcctcag gcgttgaggg cttgctcggc ctgacggagg
      421 cggcgcgaga ggacggcgag caggttccgc gtcaggtcgg gaacttccgc gaggacgcgc
      481 gagaactcac ggcgggggat gacgagcagg cgcaccggcg tctccgcgac gacggtggcc
      541 gagcgccgct caccgtcgag caggctcatc tcgccgaaga agtcgccggg ccccagccgc
      601 gggcgcttgc gcgaggacac ctccgcccgc gcgcgaccgt cgatgatcac gaagaactcg
      661 tcgccgggat cgccggcgcg cgtgaccacc cctcccgcgg gcagctcccg gatctccgcg
      721 atctgcgcga cggcgcggag ctggcgccgc gagcactcgg cgaggagcgg cacgcgctgg
      781 agctgctgga cctttgcgtc ctggctcacg cgccagccga agaagccgcg cttcggcccc
      841 ccgcccggcg gcgggatcgt gccgtccatc atccggccga ccagctccac ggctacagcc
      901 cgtacaggcg gcgcgcgttg tcggcgaaga tcttctgctt ctgccggtcg gagaggtcgc
      961 cacgcgcgcg gatctcctcg acggactgcg ggtagctgtg gtcccagtgg gggaagtcgc
     1021 tcgcgtagac gatctggttc tcgccgacga gcttcacggc ctggggcagg agccactcgt
     1081 cggcttcgca ggagaagtag atcctccccg agcggatgta ctcgctcggc ttcttcttga
     1141 gtgccggcgc ctcgctcgcg cgcttggcgt actcgtcgtc catgcgctcc atccagtacg
     1201 gcgcccagcc gcagccggcc tcgaggaagg ccaggcggag gtccgggaac cgctcgggga
     1261 tgccctcgaa gacgatcgag gtgagctggc gcatctgacc gaaggcgtgc gagcacgtgt
     1321 gggcctggat gaagcgcggg aagagatcga cgccaccgcc acccaggtgc gaacccgagg
     1381 cgtggatgcc gagcatgacg ttgagccgct gggcctcctc gtagatcggc gcgaaccgct
     1441 cgtcgccgag cacgtgggtg ccgtcggcgg cgagcatggc gcccacgaga ccgcactcgc
     1501 gcacggcgcg gcgcagctcc ttcgccgcgg cgtcggggtc ttgcacgggc aggagcgcga
     1561 cgcccttgag gcgcgggctc acccgcacga actcctcgtg gaggaacgtg ttgtaggcgc
     1621 ggcagagcgc caccgcccac tcgcggtcct tcaggaagct catgaagagg ccgagggtgg
     1681 ggtagagcac cacgctctcc atgccgccgc ggtcgagcgc ttgcagccac tcgtccgcgg
     1741 taccggccca gtcgcccagc gtgccgagca gccgccggtc ccagccgtcc tggggcaccc
     1801 acaggaaccc ggtcggccgc cggcgatagg gctcgtcgac gtacttgaga agctgctcct
     1861 ggctctccgt cacgtgtccg tcggcgtcga cgatcgtgaa gctcatcacc gcactcctcc
     1921 cgtcttcacg gcttcttgca gatgacgttc agcagggcct ggcgcttgtc ctcgcgcacc
     1981 acgcgcagcg cgcgggcgag cgcgttcgga agatccgcgg ggtcttcgac ccgctcgccc
     2041 cagccgccgc acgcctcgca gatcttctcg tagtcgggcg ccgggtcgag gtcgctcagc
     2101 ggtatggtct tggttcgcat cgaccagccc tccggcgcgt gcgactgcac cgagcgcctc
     2161 accgcgttcc acacgcggtt gttgaagacg acgaacagca cgggcaggtt gtgggcgcgc
     2221 gcggcccagt gggcggccgt cgggacgccg aagatgtagg cgccgtcgcc gacgcagctg
     2281 acgaccgtcc ggtcgggcgc ggccagcttg gcgccgagcg cggctccgag gccccagccg
     2341 agcccggagg cgggtgactg gctgaagtac gagcccggga ccgacagacg ggtgtgcgcg
     2401 aggtcgaggt cgtactcgtt gacgacgatc gtgcggtcgt cgagcacgtc gccgaccgcg
     2461 cgcgacagcc acgccatgtc gatgggccgg tcgttgcgca cggcctgggc ccgcgcggcc
     2521 gccgcctcgg tcatccggcg atgctcgccc tcccaccgcc cgcggcgcgc ggcgacgctt
     2581 cggccgtcca gccccgcccg ccgcacggcc tcggccagcg ccaccagcgt gagccggggg
     2641 gcgcccgcca gggcgtggtc ggccgcgaag ccgcggatgg gatagcgcga gtacagcgga
     2701 tcctcgccca cctggatcac cggcgtctcg ggccgcgggg acctgagcgc ggggtaccac
     2761 ggcgcgtcgg cctcgaccac gagaatggcg tcggcctcgc cgaagtacgg cgcaggatcg
     2821 ccgccggcgt ggagggcgtg ggtctgcggg aagcagaggt gcgtggaggc gtgctcgaag
     2881 accggcatac cgagggcctc ggccagcgcc accagcggcg ccacggcgcc cgggtcgcgc
     2941 cccgacgact tcacgatggc gatcggattg cgcgccgccg cgagcacgcg cgcggcgtcc
     3001 tcgaccgcgg ccggatcggt cacctgggcg gccgcaggcc gcatgcgggg agggtcgaag
     3061 tactcgatct cggtcatgcg ctcgctgagc acctcgcgcg gcagcgtgag gtacacgggc
     3121 cccgccggcg tgctctgggc gatcgcgagc gcccggtcca cgacggtctc gacctgagcc
     3181 ccgaggcgaa gctcgtagtc ccacttcacg tactcgcgga ccatcgcgcc ctggtcgaac
     3241 gactcctgcg cccagtggat gtggcggttg cgcgagcccg gcaggccccc ctccgtgatg
     3301 ggggtacggc cggcggtcat caggatgggc gcccgagcgc gcgcggcgtt gatgagcccg
     3361 cccagcgcgt tggccgtgcc gacggtgacg tgcaccatca cgacctgagc gcggcccgtg
     3421 accatcgcgt agccgtgagc catcgcgacg gccgggatct cgtggggcac cgtcatcggg
     3481 cggggcagca cctggccctg ggcctcgcgc cgcgcgtagg cctcgatgat cggcgcgaag
     3541 tcggtgccgg cgttgccgaa gaagtactcg acgtgccggg ccgccagcag ctccaggtag
     3601 gcctcggccg cgctctcgac ggacgctcgc tttcgcatct cggtcctccg acggccgcct
     3661 cggtccttcg gcgggtgaga gcccgtatcg tagagcccgt attgtataga gcgctcgcga
     3721 gcaaggcgag ggcgtcggca aggccctgac ctcgaccggc tgctcggcct cgcgcgcccg
     3781 gcctgaccgt cccgccctcg ctcctcctcc gggcggatca cgtgatcgag cgtgacgccg
     3841 cgcggccgca agtttgcgcc tgtttcggcc gacccccgct aatgtatagt tacgagacca
     3901 cacggcgagg ccctggtgtt cacgtacgcc gtccgacgcg tcctgtggct ggtgcccacg
     3961 ctcctggcga tggccctcgt cacgttcctc gtgatgcacg ccacgccggg cagtcccctc
     4021 gaccccgtgg ccgagggcgc caacccgctc tccccggagg cgcagaagaa cctcgccgcc
     4081 cactacggcc tcgaccggcc gctctggaag caattcgcca tcttcgtcgg caacgccgtg
     4141 cagggcgaat tcggcaactc gttcgtctac aagacgcgcc cggtgcgcga gatcctcgcc
     4201 gagacgctgc ccgtgtcgct cctgctcggc acgatggcgc tggtcctggc cgtcgccgcc
     4261 ggcctcacgc tcggcatcct ggccgcgatc caccagaacc gggcctggga ctacgtgtcg
     4321 gtgagcctcg ccaccttcgg tgtggccgtg ccgaacttcg tgctggccgt gttcctgatc
     4381 atcctcttct cgttcgtggt gccgctcttc ccgaccggcg gctgggactc gccgcggaac
     4441 tgggtgctgc cgaccatcac gctggcgctg gggccgatgg ggatcatcgc gcggttcacc
     4501 cgcgccagca tgctcgaggt gatccgggcc gacttcaccc tgaccgcgcg cgccaagggc
     4561 ctcggcgagg cgccggtgat cttcaagcac gtgctcaaga acgccttcat tcccatcgtc
     4621 acgctgctgg ggcccatgtt cgcggcggtg ggcaccggct cgttcttcgt cgagtcgatc
     4681 ttccgcgtgc cgggcatggg tcgcttcttc gtgctctcga tgacggggcg tgactatccg
     4741 atgatcatgg ccgtggtgct gacgtacgga gccttcctcg ccgtcatgaa cctcgtcgtc
     4801 gatctcctct atggcgctct cgacccccgc atccgctact agcgacgggg tcgtcgcggc
     4861 ggcggcgccg gtcgtggccc cgcgcgggtc gagtctcgga cgcgacgcct tccgccggct
     4921 caggcggaac cggctggcca tggccggcgc ggtggtcgtc gtgctgctgt gcctggtggc
     4981 cgtcttcgcc gacgtgctcg ccccttacgc gtacacgaag accaacttcg ggcggctcaa
     5041 cgaggcgccc tcgcgcgatc accccttcgg caccgaccag ctcggccgcg acctgctctc
     5101 gcgcatggtc tacggcgcgc gcatctcgat gctcgtcggt ctgggcgccc aggtgatcgt
     5161 ggtgctcatc ggcgtgccca tcggcgccct gtcgggatat ctcggcggcc gcgtcgacct
     5221 cctgctcacg cgcttcgtcg acgtcatgta tgcgttcccc cgcctgctct tcgtgatcct
     5281 gttgatgtcc atgctgggcg ccgggctcac caacatcttc atcgccatcg ggctcacggg
     5341 ctgggtgtcg atcgcgcggc agacccgcgc ccaggtgctg gcgctcaagg agaaggagtt
     5401 cgccgaaggt gcgcgcgcgc tcggcgccgg ctgggcccgg gtgctcggcc gccacgtcat
     5461 gcccaacgcg ctgacgccca tcgtcgtgtc gatcaccttc ggcataccgg aggcgatctt
     5521 caccgaggcc gcgctatcgt tcatcggcgt gggcatcaac ccgcccacgc cgtcctgggg
     5581 tcagatggtc ggggagggcc agcaatatct gcgctcggcc tggcacctgt gtgtgatccc
     5641 gtccatcgcc atcgcgacca cgatgctgtc gttcacgttc ttcggcgacg gcgtccgcga
     5701 cgccctcgat cccaagctca agcaatgatc gcgcgacggc agcgcgatcc gcaggccgtc
     5761 cgacggccga ggcgagcggt gaaatgctcc agatcaggag gcgaccatga ggatcccggc
     5821 catctccgac cagcagctcg acctgctcgc cacgcgcctg gccgccatcg gcgtgggccg
     5881 gcgcgacttc ctcgtcgtgg cggccggcct ggccaccatg ggcgcggccg gcttcaccgc
     5941 ccgcccggct tcggcggcgc cgaagcccgc ccccggggag aagctcgcca aggatcagca
     6001 cctgcggttc ggcggcggca gctggtacga gcaggacccc gccagccacg acttcaacaa
     6061 ggacctctat tgcaacggca ccccccagct ctgggcgggc ctcatgaagt tcaacgtcga
     6121 cttccaggcc atgccttacg tcgccagcaa ggtcacgccc aaccaggacg gctccgcgtg
     6181 gaccttcacg atccgcaagg acgcgaagtg gtcggacggt agcccctgca ccgcgcggga
     6241 tttcgagtgg tcgttcaagc gacagctcga cccggccacg gccgccccct acgcctcgtt
     6301 cctctacgac atcaagaacg gcgaggcgtt caacaagaag caagtgaccg accccgggca
     6361 agtcggggtc cgggcgaagg acgactggac cctcgaggtc acgctggagg gcccacgcgg
     6421 ctacttcccc gtgctgagcg cgtaccaggc cgcgtaccct gcgcaccggc gctcggtgga
     6481 gaaattcggc gacaagtgga cggaagccgc caacatcgtc tgcaacggcc ccttcgtgct
     6541 cgagacgtgg gagcacaaca aggtcatcgt gctgaagaag aacaagcact tcttcgacgc
     6601 caaagacgtc accctcgaga aggtcacgat cccgatcatc ccgatcgcct ccggggtgct
     6661 gccgtacgag aacaacgaga tcgatctcag ccggctccag accgctgacc tgcggcggtt
     6721 tcagtccagc ccggccaccc agaaacaggt cttccgcttt ccctatcccg gcacctggta
     6781 cctgctccca caggtcacga tgcctccctt cgacaacgtc aaggtccgcc gcgcggtggc
     6841 tcacgccatc gaccgagaga acgtggtgaa ggtgtcgcag ggcttcgcca tccccgccca
     6901 ctcgatgatc ccgcccggtt tccccggcgc tctcgacgac gccaggacgc gcagtctcca
     6961 gaagttcgac cccaagctgg cgatggccca gctcaagggc actccgttcg agggcggccg
     7021 aaactggccc aagatcaccc tcacgctgcg tgatgaggcg ctcggctcca agcccctggc
     7081 cgaggccgtg caagccgtgc tgctcgagca cctcaacatg aagaccgatc tcgaggtcct
     7141 ggagcctcgc gtgttccgtg agcggctctg gaaaaacgac ctgcagttcg tgtggatccg
     7201 ctggttcatg gactaccccg acccccacaa cgagtacttc gacaccttct acggcaagaa
     7261 gacgacgggc aagcgccagg cctggaccaa cgacgccttc gacaaggagc tggaggccgg
     7321 ccgcgacacc cgcgacccca agaagcggct cgagcactac gccagggccg agcagatcat
     7381 gcagaccgac gtcgggtacg tcccggtcgc gtgggtcgtc cgctacttcg tcgccaagcc
     7441 ctacgttcgc ggcatcgaga agaaccggca gggcgagtat gtcgtcgacg gcaacatcta
     7501 cgtggacatg ctgcgtcaca tctacatggt ggaaaaaagc tagcgcagct tgagcatgcg
     7561 gcgggcattg ccttcgtaga tcttggtctt gtcgtcggcg ctggcgcgca tcttctccat
     7621 cgccgcgatc gtgtcgcgga tgaagcccgg gccgcgctcg gggtcgaagg ggaaatcggt
     7681 gccgaacagc acgtggtcgg cgccgaagaa ctcgagcccg ctctccatgg cgtgccaggc
     7741 tccgaagagc gcggtgtcgc cgtagaacat ccggaagtag tcgatgggac gccgggcgag
     7801 ccgtcccagc gccgccagat cgtcggggtc gtcgctgcgc gagccgagct ggtcaaggcc
     7861 gccgccgacg cggccggcgc agaacgggat catcgcgccc aggtgatgcg tgatgacggc
     7921 gaggtccggg tgccggtcga agatcccgga gaacaccagc cggcccatgg ccacgctggt
     7981 ctcgtacggc cagccgaaag cccaccacat gtcgtacctc gagcgcggtt cgccggcgta
     8041 gtctgcgacc gtcgccgggc gcgccgggtg catccacacc ggcagccgca gggcggccat
     8101 ccgctcgagc accgccaggt gctcgggacg gtcgagcggc cggccgttga cgttggtgaa
     8161 gacctgtacg ccggtcgcgc ccagatcgcc cacggcacgc tcgatctcct tcatggcggc
     8221 gtcgggcgcg ttcatcggca gcgaggccac gaagccggga aagcggtcgg ggtatcgggc
     8281 cacgagcgcc gccatctcgt cgttggcgag ccgcgcgagg tccgggctcc gcgcggggtc
     8341 ggcgacgacc tcgatcggcg ggttggcgag cgtgagcacc tgcacgtagc cctcgtggcg
     8401 gtccatcatt ctcaggcgca ggtccacgtc caccagcacc gggatgcccg acatgcgctt
     8461 ctgcaacgcc atgccgggtg gcgccacggc cagcatgcgg tcgaagtagc gacgcggcag
     8521 cacgtggggg aagacgtcga tcttggtggc gacgttcgtg ctcatgagag cgggctccgc
     8581 gagatcgggt cgcggcgcgc gcgggcggcc tccgccgggc gtggcgccgc cccgcgcgcc
     8641 cggcgggcgg tgcctcgacg accgcgtccg gcacgccctt gccggcggcc agcgtggaga
     8701 agaccttccc gggcggattg gtgccgaggg tcaccgactt cggcagcgcg tcctgggcat
     8761 gcgccgacgc gcgccaggcc aggaggacgg cacgacagca tcgtcagcgg gtgtcgcacc
     8821 attacaggcg gtccccctcg tcctcgaagc gccgggcgcc ctgaatggtc cggctcccgt
     8881 gacgtcgaat ggttatcttg gagggcgtca ttctaggcca ggaggcagga gatgaaaacg
     8941 atcttcgcgg ccgtg
//
LOCUS       DATIAZ010000248         3265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_857003, whole genome shotgun sequence.
ACCESSION   DATIAZ010000248 DATIAZ010000000
VERSION     DATIAZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857003"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1890)
                     /locus_tag="VLF19_08810"
     CDS             complement(334..1890)
                     /locus_tag="VLF19_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93393.1"
                     /translation="MVALTRISRSRSLLFVVALTAAVLGATPVAAQQAPSGQATMAWH
                     VTIAPSWFDPSTAPPQITPFGMLYAIHDALVRPYPPHKMGPSLAESWKESPDGTTYEF
                     KLRSGLKFHNGDPVTTEDVKFSFERYKGAGATILHARVREVEIVDPLVVRFHLKEPWP
                     DFMTFFGTTATAAGIVVPKKYMTQVGDDGFKKHPIGAGPYKFVSHKPGVEVVLEAYPG
                     YWRRVPNIKTWVMKSVPDANTRAVLLKSGEADIGVALDGPDAEDLKRDSRMQIVSSKH
                     ASIFWIEFAEQWDPKSPWHDKRMRMAVNHALHRQRINEAACLGFCPPAGVIVPRVMDF
                     ALQVEPPPYDPQKAKQLLAEAGYPNGLDAGEFTAIPGFPTVAEATLNYLNAVGIRMRM
                     RPMERATFYAAWREKKVRGLFLTAVGNSGNAASRVESFMQSKGAYAYGGYPDIDDLFQ
                     QQARERDVKKREALLHKIQQLTIDRVMFAPIMDLRVLMGVGPRVAKHTITDVWMSPFP
                     SYEDATLKSP"
     gene            2076..2750
                     /locus_tag="VLF19_08815"
     CDS             2076..2750
                     /locus_tag="VLF19_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HSE93394.1"
                     /translation="MSDETRPLNTSIDRPAPELIKAFAALSPNELSDVLELSCVMRYE
                     IHPLWPGMPRVAGPAFTIRTGKHDNLMFHASIYLAQPGDVVVVEAGDDEMAVAGGNVC
                     AIAQRNGVAALVVDGVIRDVVESREHRFPVFARGASPIPAKRVGDGGMNVQIRCGGVV
                     VNPGDVVVADEEGIVVVPRARAEDVLAKAQTRVAADAKLSLDDWEKRHRAAVETALKA
                     RGYRLS"
     gene            2750..>3265
                     /locus_tag="VLF19_08820"
     CDS             2750..>3265
                     /locus_tag="VLF19_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HSE93395.1"
                     /translation="MRVPAKQVREQLLSVLGAWGMSDAHAGTTAEMMLETDLRGVDSH
                     GISMLPTYDREFRAGRLNVRPVFKTLREGPAMALIDADASLGHAVSVHAMNLAVDKCR
                     DSAVAVVSVVNSHHFGAAGCYARIAAERGVIGMVTSATRGVTMVPTFAAEPIMGTNPL
                     AFAAPARRNPPF"
BASE COUNT          483 a         1126 c         1142 g          514 t
ORIGIN      
        1 gagatctatc gcctcctgca ggtgccgccc gaggtcatga agcccgtgaa gacctggacg
       61 gccagtcgat agtgacgcaa tgcccacgca acctcccgaa attccagcat tccactcctc
      121 agaccgtgga agtcgggcta ggcgtcgccc tcggggagca ggccgtccgg cagtggctgg
      181 ccggcggctc cgacggcccg cagggcggtg tgcaggacgg tggccccacc tcggggcccc
      241 catgtccccg cgcgttaccg aggcgcgcca cggcgaggcc ggggcgtgcc gagccgcctg
      301 gggggcatgg gggccatgtc ggggccccca tgttcatgga ctcttgaggg tggcgtcctc
      361 gtaggacggg aacgggctca tccagacgtc cgtgatcgtg tgcttggcaa cgcgcgggcc
      421 gacgcccatc agcacgcgga ggtccatgat cggggcgaac attacccggt cgatggtgag
      481 ctgctggatc ttgtgcagca gcgcctcgcg cttcttgacg tcgcgttccc gcgcctgctg
      541 ctggaacagg tcgtcgatgt cgggatagcc accgtaggcg taggcgccct tggactgcat
      601 gaacgactcc acgcggctcg cggcgttgcc ggagttcccc accgcggtga ggaagagccc
      661 gcgtaccttc ttctccctcc aggcggcgta gaacgtcgcg cgctccatcg ggcgcatccg
      721 catgcggatg ccgaccgcgt tcaggtagtt cagcgtcgcc tcggccacgg tcgggaaccc
      781 gggaatcgcc gtgaactccc cggcgtcgag cccgttcgga tagccggcct cggcgagcag
      841 ctgcttcgcc ttctgggggt cgtacggcgg cggctcgacc tgcagcgcga agtccatgac
      901 ccggggaaca atcacgcctg ccggcgggca gaagcccagg cacgcggcct cgttgatccg
      961 ctggcggtgc aacgcgtggt taaccgccat gcgcatccgc ttgtcgtgcc agggcgactt
     1021 cgggtcccac tgctcggcga actcgatcca gaagatcgat gcgtgcttcg aggacacgat
     1081 ctgcatgcgg gaatcacgct tgaggtcctc ggcgtcgggc ccgtcgagcg cgaccccgat
     1141 gtcggcctcg ccgctcttca gcaggacggc ccgcgtgttc gcgtcgggga cgctcttcat
     1201 gacccacgtc ttgatgttgg gcacgcggcg ccagtacccg gggtaggcct cgagcacgac
     1261 ctccacgccg ggcttgtggc tcacgaactt gtaggggccg gcgccgatcg gatgcttctt
     1321 gaagccgtcg tctccgacct gggtcatgta cttcttgggg acgacgatgc cggcggcggt
     1381 ggccgtggtg ccgaagaacg tcatgaagtc cggccagggc tccttgagat gaaagcgcac
     1441 caccagggga tcgacgatct cgacctcgcg gacgcgagcg tggaggatcg tggctccggc
     1501 gcccttgtag cgctcgaagc tgaacttcac gtcctcggtg gtcacggggt caccgttgtg
     1561 gaatttcagc ccggaccgca gcttgaactc gtacgtcgtg ccgtccggac tctccttcca
     1621 cgactccgcc aggctcgggc ccatcttgtg aggggggtac ggcctgacca gcgcgtcgtg
     1681 gatcgcgtag agcatgccga acggcgtgat ctgcggcggc gcggtggagg ggtcgaacca
     1741 gctcggcgcg atcgtcacgt gccaggccat ggtggcctgc cccgacgggg cctgctgcgc
     1801 ggcgaccggc gtcgcgccga ggacggcggc ggtgagagcg acgacgaaga gaagcgagcg
     1861 gctccgggat atccgagtca gtgcaaccat attcgtcacc tgcctctttc tgaccaggta
     1921 gggggaaccg aacaaatcga cggccggccc gaggatcgcc gcaaaggccc gtccacgtca
     1981 agggggccgg tggcttccgg ggcggacggt gtatcatcgc ccttccagga cggaattcac
     2041 ggtccggccg tgccccctga cccgggagac gacgcgtgag cgacgagacc cgccccctca
     2101 acacgagcat cgaccggccc gcgccggagc tgatcaaggc cttcgcggcg ctgtcgccga
     2161 acgagctcag cgacgtgctc gagctgtcct gcgtgatgcg ctacgagatc catcccctct
     2221 ggcccggcat gccgcgcgtc gccgggccgg ccttcaccat ccgcaccggc aagcacgaca
     2281 acctcatgtt ccacgcgtcc atctacctgg cccagcccgg cgacgtcgtc gtggtcgagg
     2341 ccggcgacga cgagatggcg gtggccggcg gcaacgtgtg cgccatcgcc cagcgcaacg
     2401 gcgtggccgc cctcgtcgtg gacggcgtca tccgcgacgt cgtggagtcg cgggagcacc
     2461 gcttccccgt gttcgcgcgc ggcgcctcgc cgatccccgc caagcgcgtg ggcgacggcg
     2521 gcatgaacgt gcagatccgg tgcggcgggg tcgtggtcaa cccgggcgac gtggtcgtgg
     2581 ccgacgagga gggcatcgtc gtcgtcccgc gcgcccgcgc cgaggacgtg ctggccaagg
     2641 ctcagacccg cgtggccgcc gatgcgaagc tcagcctgga cgactgggag aagcgccacc
     2701 gcgccgccgt cgagaccgcg ctcaaggccc ggggctaccg gctgtcgtga tgcgcgttcc
     2761 cgccaagcag gtccgcgagc agctcctttc cgtcctgggc gcctggggta tgtcggacgc
     2821 gcacgccggc accaccgccg agatgatgct ggagaccgac ctgcgcggcg tggactcgca
     2881 cggcatctcg atgctgccga cctacgaccg cgagttccgc gcggggcggc tcaacgtgcg
     2941 tccggtgttc aagaccctcc gggaggggcc ggccatggcc ctgatcgacg ccgacgcgtc
     3001 gctcggccac gcggtctccg tgcacgcgat gaacctcgcc gtggacaaat gccgcgacag
     3061 cgccgtggcc gtcgtgtccg tggtcaactc gcatcacttc ggcgccgctg gctgctacgc
     3121 gcggatcgcc gcggagcgcg gcgtcatcgg catggtcacc tcggccacgc ggggcgtgac
     3181 gatggtgccg acgttcgccg ccgagccgat catggggacc aatccgctgg cgttcgccgc
     3241 gccggcgcgg cgcaaccccc cgttc
//
LOCUS       DATIAZ010000249        20465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_331546, whole genome shotgun sequence.
ACCESSION   DATIAZ010000249 DATIAZ010000000
VERSION     DATIAZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20465
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331546"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VLF19_08825"
     CDS             <1..321
                     /locus_tag="VLF19_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93396.1"
                     /translation="YEPDEEATYGIAAATVFYPLCWAAEAWLAWRLGSGLALAVFLVA
                     LLPSAFFALGWRDRLESFRTDALVLLRLVRDPRLGPRLGTQRRALLEEMDALARAVPE
                     DPLA"
     gene            complement(409..1626)
                     /locus_tag="VLF19_08830"
     CDS             complement(409..1626)
                     /locus_tag="VLF19_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93397.1"
                     /translation="MLTRRALASLLALALGLGLAGPAGAQQAPGVTATEIKIGNTNPY
                     SGPASAYGTIGKAITAYFKKVNEEGGINGRKVNFISHDDSYSPPKTVEMVRRLVEQDQ
                     VAFVFQTLGTPTNSAIHRYMNQQKVPHLFVATGATKWGDPKNFPWTMGWQPTYQTEGK
                     IYAQYVLKNVPDAKIGILYQNDDYGKDYVKGLKDGLGDAAKKLIVLEQSYEVTDPTID
                     SQIVNLKNSGANVFYNVTIPKFAVQAIKKSADIGWKPLHLLNSVSQSVGVVLKPAGLD
                     ASKGLITVFALKDPTDPQWKNDQGYKDWLAFMKKYYPEGNTDDTFNVYGYSVAQTMVH
                     VLKQCGNDLSRANIMKQAASIKDLSLPMLLPGIKINTGPNDFYPIEQEQLAKFDGERW
                     ALFGEIYDGAKMK"
     gene            complement(1662..2603)
                     /locus_tag="VLF19_08835"
     CDS             complement(1662..2603)
                     /locus_tag="VLF19_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93398.1"
                     /translation="MVLVALAAACVLPFTVSGYRIFQFTQVGIYAIALLGLNILTGYN
                     GQISLGHGAFYAVGAYTAAIMIDRWSVPYGWTVPAAGVLCFVAGFLFGIPALRLEGLY
                     LALATFALALAVPQILKYFEHWTGGSQGIVLSKPEAPFGLPLSADQWLYFITLAWLVV
                     LFVLAANLLKGRTGRAIVAIRDHPIAAEAMGVNSALYKSLTFGVSAAYTGVAGALSGL
                     AIAFVAPDAFNVFLSITFLVGVVVGGLASISGAIWGALFTQFVPNWAQDISKAAPWAI
                     YGVFLIVFMYVMPHGISGTVRLAWRRLRRPAGTRPAP"
     gene            complement(2683..3573)
                     /locus_tag="VLF19_08840"
     CDS             complement(2683..3573)
                     /locus_tag="VLF19_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93399.1"
                     /translation="MEIFFQQVVSGIATGGIYGALALALVMIYQATDVVNFAQGEMAM
                     FSTYLAWSLLDAGVPYWAAFFGTLAIALVGGIVIERVVIRPVENAPILAIVIVCIGLL
                     VILNSVAGWIYSYIQKPFPSPFPERPIYVGGTDGIVFGAHDLGAIGVTLVVLTFLYLF
                     FRYTPLGLAMRAAAQNPVSSRLCGVRVGWMLAMGWGLAAMVGAVAGMMIAPVVFLDPN
                     MMAGILIYAFASATLGGFTSPGGAVLGGLIVGVAENLVGTYVKFIGTELKLTVALAII
                     IAVLLVKPSGLLGRPVVRRV"
     gene            complement(3582..4295)
                     /locus_tag="VLF19_08845"
     CDS             complement(3582..4295)
                     /locus_tag="VLF19_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93400.1"
                     /translation="MPPLLEVEDLQAHYGFTKALHGISFAVEERGITTILGANGAGKT
                     TTLRAVCGMVRTSGAIRFAGQRIDGRATEAVVRLGIAHAPEGRGTFVQLTVEENLRLG
                     AWGRRDRAGLARDIERMYEHFPVLRERHRQLAGTLSGGEQQMLAVARALMRRPRLLLL
                     DEPSLGLAPLIVREIFGIVGAINREEGVSVLLVEQNAALALDLAGHAYLLETGRVVRS
                     GPAADLRRDDAVRRSYLGY"
     gene            complement(4297..5085)
                     /locus_tag="VLF19_08850"
     CDS             complement(4297..5085)
                     /locus_tag="VLF19_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93401.1"
                     /translation="MSGPGDPSTPLLTVRDVTVRFGGIVALDGVSFDMQEGQIVGLIG
                     PNGAGKTTLFNCLSRLYTPERGDIRFGGRSLLTLPAHRIATLGIGRTFQNLALFGSMT
                     VLQNVMIGVHGRTRSDFLSNALRLPSVAREEAATRAAAVELIRFLGLEAVALHPAAGL
                     PFGTLKRVELARALAGRPRLVLLDEPAGGLNHEEVAALAVLLRAIRDQRGVTVLLVEH
                     HMGLVMQVSDRVVVLDFGRKIAEGAPAEVQRDPEVIRAYLGSAA"
     gene            5214..5495
                     /locus_tag="VLF19_08855"
     CDS             5214..5495
                     /locus_tag="VLF19_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93402.1"
                     /translation="MSLDAAREQWWVGLREHERAAYTAEGRDFTRDETEFRRGFEAAL
                     SFGREVPGYGEVAAQLRARHPDPGDDDAFRRGYERGREWVLSHRRDRAA"
     gene            5516..5929
                     /locus_tag="VLF19_08860"
     CDS             5516..5929
                     /locus_tag="VLF19_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93403.1"
                     /translation="MRSILRDLTGLVPDVAGRLPDLRDLPFRRQRGLSATGVAGMGLI
                     AAGLGAALMFMFDPEAGRRRRARARDRVTSFVRRSSVAMDGASRDMANRARGLVIEMR
                     SRLVPPPAGTAGTARAGEPFGERGSGSLHQGRTQP"
     gene            complement(5970..6683)
                     /locus_tag="VLF19_08865"
     CDS             complement(5970..6683)
                     /locus_tag="VLF19_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSE93404.1"
                     /translation="MRLAAIADLHCSRLPADLLARYLTSIGDGADALLLCGDLTDRGQ
                     EDEARALAQTLRTAVRIPILAVLGNHDYEAGKPDTVKAILTDAGVHMLDGEAVEIGGV
                     GFAGAKGFGGGFGPRALQPWGEELMKRFVHEAVEEALRLESALAKLRTSRVVALLHYA
                     PVEDTVKGEPPEIYPFLGSSRLEDPLTRHPVTAVFHGHAHHGAPEGRTRNDAPVYNVA
                     IPLLQRAFPDRPPLRIVEV"
     gene            complement(6661..7473)
                     /locus_tag="VLF19_08870"
     CDS             complement(6661..7473)
                     /locus_tag="VLF19_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93405.1"
                     /translation="MAEGRPQAARFYVAALHALTDGDVQFLVGGAYSFERYTGIARDT
                     KDLDVFVRREDFDRALGALAAAGYRTEMAFPHWLGKAYDGDDVVDVIFGSGNGCAGVD
                     DEWFAHAVDEAVLGVPVRLCPPEETIWSKAFIMERERYDGADVAHLLRACAGGLDWRR
                     LLRRFGPHWRVLLAHLVLFGFVYPIENGRIPRQVMAELSRRLGRDAGRPVSAAPVCFG
                     TLLSRAQYLMDVETWGYRDGRLEHGTMTEDDVAVWTSAIEDGQPKNAAGGDR"
     gene            complement(7572..7715)
                     /locus_tag="VLF19_08875"
     CDS             complement(7572..7715)
                     /locus_tag="VLF19_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015301316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93406.1"
                     /translation="MDDGAIDRGGGVIEWVHFCERCGARMEEQKCKILCRNCGLARDC
                     SDP"
     gene            7857..8354
                     /locus_tag="VLF19_08880"
     CDS             7857..8354
                     /locus_tag="VLF19_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93407.1"
                     /translation="MSEAWEDGERLRHIFDETRVVRRPLRGIIAGYHVLPYILVGPER
                     DQPGRSVEVRGRIKVSPRLVITPGRDGPTYGELFGERELMDHTLLARVFSFRYAQRVS
                     LESDELTIRKQTRDPEGHLERVLEELAQREVIDTGVIVSPDPRFYPVSIDRFIREILD
                     EEFRE"
     gene            complement(8362..9549)
                     /locus_tag="VLF19_08885"
     CDS             complement(8362..9549)
                     /locus_tag="VLF19_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSE93408.1"
                     /translation="MHPGALDGVFVLDLTNQLSGPYCTMILADLGADVVKVERPDNGD
                     DMRASGPHVRGESAPFMTVNRNKRSLTLDLKAPDGLAVARRLAARADVVVENWRPGTA
                     ARLGLGWEDVHALNPRTVYCSISGFGQTGPYAARGGFDRIAQGMSGIMAINGDETGPP
                     LPVPVPISDIGTGMFAAIAILAALQARVRTGEGQAIDASLLETPIAWSVYESSAYFAT
                     GEPPRRLGPGHRTNAPYQAFRTADGWLNLGGGAPGFWPRICAVLGVEPLLEDPRFTTP
                     ALRVKHRAELEALLEARFRTATTAVWLARLEGAGVPAGPILGYDEVFADPHVRHRAMA
                     VEVEHPTAGKGRVLGVPVKLSATPGAVRRPAPALGQHTDEILRELGYGPAVIADLHAR
                     KVV"
     gene            9657..10835
                     /locus_tag="VLF19_08890"
     CDS             9657..10835
                     /locus_tag="VLF19_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSE93409.1"
                     /translation="MRLRGLGALVCAVAVLAGGLPLVEAQAPIRIGASLSLTGTYAKL
                     GNYQNEGYKLCLKQVNDKGGLLGRRVELVMYDDQSTPATGVRLYEKLITEDKVDAVIG
                     PYSSPITEAVANVTEKYKKVMVTPLAATTSIFKKGRKHIFMIISPAEVYLEGLVDMAA
                     KRGLKTVAVVNEDTLFSKAAAAGAADLARKRGMQVVFQEAYPKGNTDFSALLTKVRAV
                     NPDVIAAATYFDDAVALTRQMRELNVAPKMYGVTVGGDLPEFHDLLKQNADYIYGATQ
                     WEPTLPYPGNREFFEAYKKEFNREPVYHSAAGYGGCLIYAEAVKRAGSLDSDKVREQL
                     LKLETRTAFGGYKVDQDGFQIAHKMVTFQWQDGKKVTVWPDELAQAKARFPTPAWNQR
                     "
     gene            10954..11832
                     /locus_tag="VLF19_08895"
     CDS             10954..11832
                     /locus_tag="VLF19_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93410.1"
                     /translation="MNIVITPAMLGQVIVSGILAGSLYALVSLGLALIYGVMRVINIA
                     HGPLLMLGAYTTYFLVAGTGMNPFLTIPVAMAALFVVGVLLQRTLVFRVVDAPELSSL
                     LLTFGISIAIVNLAQLVFSSDLRAVEYITGSWVMAGLAFSKSRVIAFAFAAAVTALAF
                     LFLKSTRLGKAIRATSQSREVAMVCGIDVRRIHLLTFGFASALAAAGGALLAVIVAIQ
                     PEMGGVWTFKSFLVIVLGGAGNYPGALLGGMLLGLIEQLASLFLTTQLSEVVAYVLLV
                     LVLLVRPTGLLGGRQT"
     gene            11829..12749
                     /locus_tag="VLF19_08900"
     CDS             11829..12749
                     /locus_tag="VLF19_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93411.1"
                     /translation="MKRAFLAALVVALAALAVYPAFGTGYGVRSMLQIFMWIALAGSW
                     NLISGLTGYVSFGHVAFFGAGAYTGAILVTQHGWPWPVAALAGGLAALVLAVLIGWPC
                     LRLKGPYFAIAMLGLNEVLRALVSYFEGVTGGGNGLSLPTLDASVPIYYVMGLLAVAV
                     TGVTSLIITSRFGLRLMTIREDELAAEAMGIDTFRHKLYAFLLSAAGPGVAGALTARD
                     QGYIEPISVFPLATTVTMIVMVLFGGKGTVWGPVLGAVVLFVAQEIVWARYPYVHPLL
                     FGAIIVAVVLAMPRGVLGLLQLKYRLPRTI"
     gene            12749..13456
                     /locus_tag="VLF19_08905"
     CDS             12749..13456
                     /locus_tag="VLF19_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93412.1"
                     /translation="MSPPALEVQDVRKSFGGVTAVNGVSLRLDPARIYGLIGPNGSGK
                     TTLFNCITGVERRDAGHVRLHGERIDGLRPYQIAQKGVGRTFQVIRVFPELTALENLL
                     VVTRGDREAAVRRALELLGFVKLERLRDEYAGNLSYGQQKLVEFVRVLMRDPSLILLD
                     EPAAGVNRTLLNDLLGAVRQLRDQGKTVLLVEHDMKVVMGLCETVFVLDHGEKIAEGP
                     PGVIQSDERVIEAYFGR"
     gene            13434..14933
                     /locus_tag="VLF19_08910"
     CDS             13434..14933
                     /locus_tag="VLF19_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93413.1"
                     /translation="MKRTSAGSLADACRRAVAAGLLLGLAATVLRMAWRSRGWPLVHD
                     APIMHYVAWRIGEGAAPYRDLFDMNFPGTYLLHLAVVRLLGPGDAAWRGFDLAWLAAG
                     AVAVAAFAAPWGRAAAGGGALFFAAHHLAGGAGQAGQRDFLLCPFLLLGALGVARWLE
                     QRRASSLAWGGLVLGAGVTIKPHAALLAGTLAVVIALGAWRSGSGVPVAAFGGATALA
                     PLAVVAWIAAVGALPAWRSIVFEYLLPLYSQLARPEYWGFHRWHVWIPIALAVGASLT
                     SALVSRRFGARHGVAVLGLVYGVAHYVGQGKGWEYHIYPAAAFAAVLLFCELEALLRA
                     RRPVAAIALAACLLATVVMLDTKAAEAAGSAETGWIARKARRVVALATDLRARLAPGD
                     LVQVLDTTGGGIHALLRLHARQPTRFLYDFHFFHDADAPVIRALRDELVRGLDARPPR
                     FVVFFREGWPAGGDERIAAFPELATRLDRGFRLDTRGDGYVIYAKRDDP"
     gene            14911..15558
                     /locus_tag="VLF19_08915"
     CDS             14911..15558
                     /locus_tag="VLF19_08915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE93414.1"
                     /translation="MRSETIRSIIRAYDDPVVQAYCWVRFWILRQRFLDEIGQYLPEA
                     GPVLDVGCGFGLFSLYYAATAPGRVVRGLDLNGRRIALARRAAARLGLENVAYEEADA
                     RHYKGDTEVAAAYMLDIVHHVPRESVPPLLAQLRRSLPAGGLLLVKDVDTRPAPKRWF
                     TWALDRLMSPRTPVAYWSAEALTEALEAARFRVRRHLMVDVLPYPHVLYVCEARP"
     gene            15612..16277
                     /locus_tag="VLF19_08920"
     CDS             15612..16277
                     /locus_tag="VLF19_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93415.1"
                     /translation="MDILHGVALRVDTGEIVSVIGPNGAGKSTAFKTIVGLLHPREGR
                     IRFNGEDITGLRPDLVLRRGLAYVPQGRIVFPQMTVLENLEMGAYIERDPKRIVQALE
                     RVYALFPVLAERKRQRAGTMSGGEQQMVAIARALMTTPALILLDEPSLGLSPKFVTLI
                     FDKLVEMKRAGYTLMLVEQNAARALAIADRGYVLELGRNRFEGTGPRLLADAEVKRLY
                     LGG"
     gene            16274..17119
                     /locus_tag="VLF19_08925"
     CDS             16274..17119
                     /locus_tag="VLF19_08925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSE93416.1"
                     /translation="MSARVSVLMCVHNGLALTRACLDSLCAATEPFELVVVDNASTDQ
                     TPAFFARASYPFPIRYRREERNEAVIARLNTAWRLATTDHLCFLHNDTEMIEPAWLAR
                     LLAALAEPRAGLAGLYGAKRLRRDGRLMGRTIVHALAEGPTVRPPWEEVAFVDSVCMC
                     LRRDVLEAVGGFDEGYGFYHGHDRDLSLAVRETGRRCLVAYAPFHHRGGGTRAHEFGA
                     RPDRERADLDLRTAAMARFARKWAHRLPCDVRSLRERVRDWVRTTARGRVSRAGDPFV
                     RRWTG"
     gene            complement(17184..19283)
                     /locus_tag="VLF19_08930"
     CDS             complement(17184..19283)
                     /locus_tag="VLF19_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE93417.1"
                     /translation="MSYRLFHFAGGQAARLAERLQECRRTVLDERAEPGVVTAPAVIL
                     VDAQVAQLVRALALKGADPAIAIVVLGDAAHLPLTPSEHVHAYLLSTLPMSILVQTLH
                     NAAVHAQLHLEQAQMRVNLERLATEFSSLNAIGIALSSERDPTALLRLVVARARELTH
                     ADAGSLYLVEEDAAGRRRLRFTVVQNDSAEAPFEGGTLAIGPTSVAGHVAFTGQPQNL
                     PDAYTPPAGSAFLVDPVYDTETGYRTKSMLVVPLKTPAARVIGVMQLINCKADPAVRF
                     TSREHIEREARPFPARFEELATSVASQAAVAIENARLYAELQAALGQLAASQRGVVRA
                     ERLRALGDMAGGLSHDFNNTLAAILGRAQLLLAQVEDPDVRRQLEVVEEAALEAARTL
                     RRVQEFVRMRRERPFLTVELGALAQEVVDATRPRWRDEAAARGIAYDVRVDATPADLV
                     AGDPLELREVLMAVVLNALDAMPAGGRLTLTTVPESDRVRCTVTDSGVGMTDEVRERV
                     FEPFFTTKGARGSGLGLSVAYGILMRHGGEVDVDSRPGEGTAVSIRLPVATPDIAASG
                     VEETVPPSAEPLAGTHALVVDDEEAVRHVLEDALTALGCRVTACGDGTTAIARLDAEP
                     FDVVVTDLGMPGASGWDVARHVRRVRPGARIVLVTGWGAQLSSADLAAGGIDQLVLKP
                     FEIDQIESAVRKALAGR"
     gene            complement(19370..19445)
                     /locus_tag="VLF19_08935"
     tRNA            complement(19370..19445)
                     /locus_tag="VLF19_08935"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19410..19412),aa:Ala,seq:ggc)
     gene            complement(19512..19598)
                     /locus_tag="VLF19_08940"
     tRNA            complement(19512..19598)
                     /locus_tag="VLF19_08940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19562..19564),aa:Leu,seq:caa)
     gene            19890..>20465
                     /locus_tag="VLF19_08945"
     CDS             19890..>20465
                     /locus_tag="VLF19_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HSE93418.1"
                     /translation="MGERTFAEDVKQLGYGAGQVLRGEGVLAITKALLQSGVSYVGGY
                     PGAPVSHLLDVMADANEPLLKPMGIYFEQSGSEAAAAASLGASINYPMRGAVTWKSVV
                     GTNVASDALSHVASAGVIGGALIVIGEDYGEGASILQERTHSAALKSSVPLIDPKNSL
                     PSFARFVEEGFGLSEACHEPVFFSIRIRACHM"
BASE COUNT         2908 a         7175 c         7298 g         3084 t
ORIGIN      
        1 tacgagccgg acgaggaggc gacctacggc atcgccgccg cgacggtctt ctatccgctc
       61 tgctgggcgg ccgaggcgtg gctcgcgtgg cggctcggga gcgggctcgc gctggccgtc
      121 ttcctcgtcg cgctgctccc ctcggccttc ttcgcgctcg gctggcgcga ccgcctcgag
      181 agcttccgca ccgacgcgct cgtgctcctg cggctcgtgc gcgatccgcg gctggggccc
      241 cggctcggca cgcagcgccg ggccttgctc gaagagatgg acgcgctcgc tcgggccgtg
      301 cccgaagacc ccctcgcgtg agtcgtcgcc cgtcgcggca cgcgccagaa gggagggtgg
      361 gaaggcatcg gagagggcgt cgcgccccct ccgatgcggt cgaccgagct acttcatctt
      421 cgcgccgtcg tagatctcgc cgaagagcgc ccagcgctcg ccgtcgaact tcgcgagctg
      481 ctcctgctcg atcgggtaga agtcgttggg gcccgtgttg atcttgatgc ctggcagcag
      541 catcggcagc gagagatcct tgatgctcgc cgcctgcttc atgatgttgg cccggctcag
      601 gtcgttgccg cactgcttga gcacatgcac catggtctgc gccaccgagt atccgtagac
      661 attgaacgtg tcgtcggtgt tgccctcggg gtagtacttc ttcatgaagg ccagccagtc
      721 cttgtagccc tggtcgttct tccactgcgg gtccgtgggg tccttgagcg cgaacaccgt
      781 gatgaggccc ttggaggcgt cgagtccggc cggcttgagc accacgccga ccgactgcga
      841 gacgctgttg agcaggtgca gcggcttcca gccgatgtcc gcagacttct tgatggcctg
      901 cacggcgaac ttcgggatgg tcacgttgta gaagacgttg gcgccgctgt tcttgaggtt
      961 gacgatctgc gagtcgatgg tggggtcggt gacctcgtag gactgctcca ggacgatgag
     1021 cttcttggcc gcgtcgccca gcccgtcttt gaggcccttg acgtagtcct tgccgtagtc
     1081 gtcgttctgg tagagaatgc cgatcttcgc gtcgggcacg ttcttcagca cgtactgcgc
     1141 gtagatcttg ccctcggtct ggtacgtcgg ctgccagccc atcgtccacg ggaagttctt
     1201 gggatccccc cacttggtgg cgccggtggc cacgaagagg tggggcacct tctgctggtt
     1261 catgtacctg tggatggcgc tgttggtcgg cgtgcccagc gtctggaaga cgaaggccac
     1321 ctgatcctgc tcgaccaggc gccgcaccat ctcgacggtc ttgggcgggc tgtaggagtc
     1381 gtcgtgagag atgaagttga ccttccggcc gttgatgccg ccctcctcgt tgaccttctt
     1441 gaagtaggcg gtgatggcct tgccgatggt gccgtaggcg gaggccgggc cgctgtacgg
     1501 gttggtgttg ccgatcttga tctccgtggc ggtcacgccc ggagcctgct gggcgccggc
     1561 cgggccggcg aggccgagac ccagcgccag ggcgagcaac gatgccagcg cgcgacgggt
     1621 gagcatggga gcctcctgtc tggtcctgtt ctgtagaacg gtcatggcgc gggccgggtt
     1681 ccggcggggc gccggaggcg ccgccacgcg agccgcacgg tgcccgagat gccgtggggc
     1741 atgacgtaca tgaacacgat gagaaacacg ccgtagatgg cccagggggc ggccttcgag
     1801 atgtcctggg cccagttcgg cacgaactgg gtgaagagcg cgccccagat cgccccggag
     1861 atcgaggcga ggccgccgac gacgacgccg acgaggaagg tgatcgagag gaacacgttg
     1921 aacgcgtcgg gcgccacgaa ggcgatggcc aggccgctga gcgcgccggc cacgccggtg
     1981 tacgccgcgc tcacgccgaa cgtcagcgac ttgtagagcg cgctgttcac gcccatcgcc
     2041 tcggccgcga tcgggtggtc gcggatcgcc acgatggccc gtcccgtgcg gcccttcagg
     2101 agattcgcgg cgagcacgaa caggacgacg agccaggcca gcgtgatgaa gtacagccac
     2161 tggtcggccg acagcggcag cccgaagggc gcctcgggct tgctcagcac gatgccctgc
     2221 gacccgcccg tccagtgctc gaaatacttg aggatctgcg gcaccgccag cgcgagcgcg
     2281 aacgtggcca gcgccagata caggccttcg agccgcagcg ccgggatgcc gaagaggaag
     2341 cccgcgacga agcagagcac gccggcggcc gggaccgtcc agccgtaggg cacgctccag
     2401 cggtcgatca tgatggccgc ggtgtacgcg ccgaccgcgt agaaggcgcc gtggccgagc
     2461 gagatctggc cgttgtagcc ggtcaggatg ttcaggccga gcagcgcgat cgcgtagatc
     2521 ccgacctggg tgaactggaa gatccggtag ccgctgaccg tgaatggcag cacgcaggct
     2581 gcggcgagcg ccacgagcac cacggcgaac cgccaggggc gcacggcggc ggccgcgtgg
     2641 gcgtcgcggc gggccacggc gacgtccggc acgctcacgg cctcacaccc ggcgcaccac
     2701 cggccgcccg aggagcccgc tcggcttgac gaggagcacc gcgatgatga tcgccagcgc
     2761 gacggtgagc ttcagctcgg tgccgatgaa cttcacgtaa gtgccgacga ggttctcggc
     2821 cacgccgacg atcaggccgc cgagcacggc gccgccgggg ctcgtgaagc cgcccagcgt
     2881 cgccgaggcg aaggcgtaga tgaggatgcc cgccatcatg ttcgggtcga ggaacacgac
     2941 cggggcgatc atcatgccgg cgacggcgcc caccatcgcc gccagccccc agcccatggc
     3001 cagcatccag cccacccgga cgccgcagag ccggctggag acggggttct gcgcggccgc
     3061 gcgcatggcc aggccgagcg gcgtgtagcg gaagaacagg tagaggaagg tcagcacgac
     3121 cagcgtgacg ccgatggcgc cgaggtcgtg ggccccgaag acgatgccgt cggtgccgcc
     3181 gacgtagatc gggcgctcgg ggaacgggct ggggaatggc ttctggatgt aggagtagat
     3241 ccagccggcc acgctgttca ggatcacgag caggccgatg cacacgatga cgatggccag
     3301 gatcggcgcg ttctccacgg gacggatcac gacgcgctcg atcacgatgc cgccgacgag
     3361 cgcgatggcc agggtgccaa agaaggccgc ccagtagggc acgcccgcgt cgagcagcga
     3421 ccaggcgagg tacgtgctga acatcgccat ctcgccctgg gcgaagttca cgacgtcggt
     3481 cgcctggtag atcatcacga gcgccagggc gagcgcgccg tagatgcccc ccgtggcgat
     3541 gcccgacacg acctgctgga agaagatctc caccgcgcgg ctcagtatcc caggtaggag
     3601 cgccggaccg cgtcgtcgcg gcggaggtcc gcggcggggc ccgagcgcac cacgcggccg
     3661 gtctcgagca ggtaggcgtg gccggcgagg tccagggcga gcgcggcgtt ctgctccacc
     3721 agcagcacgc tcacgccctc ctcccggttg atggcgccga cgatgccgaa gatctcgcgg
     3781 acgatcagcg gcgccagccc cagcgagggc tcgtcgagca gcaggagccg gggccggcgc
     3841 atgagcgcgc gcgccaccgc gagcatctgc tgctcgccgc ccgacagcgt tcccgcgagc
     3901 tgccggtgcc gctcgcgcag cacggggaag tgctcgtaca tccgctcgat gtcgcgggcg
     3961 agcccggcgc ggtcgcggcg accccaggcc cccagccgga ggttctcctc gaccgtgagc
     4021 tgcacgaagg taccgcgtcc ctcgggggcg tgggcgatgc ccaggcgcac gaccgcctcg
     4081 gtggcccggc cgtcgatccg ctgccccgcg aagcgaatgg cgcccgacgt gcgcaccatg
     4141 ccgcagaccg cccgcagcgt ggtcgtcttg ccggcgccgt tggcgccgag aatcgtggtg
     4201 atgccgcgct cttccacggc gaaggagatg ccgtggaggg ccttcgtgaa gccgtagtgc
     4261 gcctggaggt cctcgacctc gagtagcggc ggcacgtcag gccgcgcttc cgaggtaggc
     4321 gcggatcacc tccggatcgc gctggacctc ggccggcgcc ccctccgcga tcttccggcc
     4381 gaagtcgagg accacgaccc ggtcggagac ctgcatgacg aggcccatgt ggtgctcgac
     4441 gagcagcacc gtgacaccgc gctggtcgcg gatggcgcgg agcagcaccg ccagagcggc
     4501 cacctcctcg tggttgaggc cccccgccgg ctcgtcgagc agcaccagcc gtgggcggcc
     4561 cgccagcgcg cgggccagct cgacgcgctt gagcgtaccg aagggcagcc ccgccgcggg
     4621 gtgcagcgcg acggcctcga gcccgaggaa gcggatcaac tccacggccg cggcgcgcgt
     4681 ggccgcctcc tcgcgcgcca ccgacggcag ccgcagcgcg ttgctgagga agtccgagcg
     4741 cgtgcgccca tgcacgccga tcatcacgtt ctggagcacg gtcatggagc cgaagagcgc
     4801 caggttctgg aacgtgcgac cgatgccgag cgtggcgatc cggtgggccg gcagcgtgag
     4861 gagcgagcgc ccgccgaagc ggatgtcccc gcgctcgggc gtgtagagcc ggctgagaca
     4921 gttgaagagc gtcgtcttgc cggcgccgtt cgggccgatg aggccgacga tctggccctc
     4981 ctgcatgtcg aacgacacgc cgtcgagggc gacgatgccg ccgaaccgca ccgtcacgtc
     5041 tcggacggtc aacagcggag tagaggggtc tccgggaccg gacatggcaa gaagtgggcc
     5101 tagtttatag agagggtgtg tccatcctgt caaccgcctt ctgccccggc cggtccggcc
     5161 accaggcgcg gtcgctggca cgccatccgc atccaccagg acccatgaat accgtgagcc
     5221 tcgatgcggc gcgcgagcag tggtgggttg gcctgcgcga gcacgagcgc gccgcctaca
     5281 ccgccgaggg acgggacttc acgcgtgacg agaccgagtt ccggcggggc ttcgaggccg
     5341 cgctgagctt cggccgggag gtgcccggct acggcgaggt cgctgcacag ctccgcgccc
     5401 gccatccgga ccccggtgac gacgatgcgt tcaggcgcgg ctacgagcgg ggacgcgagt
     5461 gggtcctttc gcaccgccgg gaccgcgccg cgtgagaacg atgagggagg acgccatgcg
     5521 aagcatacta cgcgatctca ccggcctggt gcctgacgtg gccggccgcc ttcccgacct
     5581 gcgtgacctg ccgttccggc gccagcgggg cctgtcggcc acgggcgtgg ccggcatggg
     5641 cctgatcgcc gccggtctcg gcgcggccct gatgttcatg ttcgaccctg aggcgggccg
     5701 ccgccgtcgg gctcgcgcgc gcgaccgggt gacgagcttc gtgcgccgct ccagcgtggc
     5761 catggacggc gcatcccgcg acatggccaa ccgtgcgcgc ggcctcgtca tcgagatgcg
     5821 ctcgcgcctc gtcccgccgc cggccggcac ggcaggcacc gcgcgcgccg gggagccctt
     5881 cggcgagcgc ggttcaggat cgctccacca gggccgcacc cagccgtagg gacacactcg
     5941 acgcgccggt cgccggccga ccgctgtcac tagacctcca cgatcctgag gggcggccgg
     6001 tcggggaagg cccgctgcaa gagcgggatg gccacgttgt agaccggcgc gtcgttccgc
     6061 gtccgtccct ccggggcgcc gtggtgagcg tggccgtgga agaccgccgt caccggatga
     6121 cgtgtgagcg gatcctcgag ccggctcgag ccgaggaagg gatagatctc ggggggctcc
     6181 cccttgacgg tgtcctccac cggcgcgtag tggagcaggg cgacgacccg cgaggtgcgc
     6241 agcttggcga gcgcagactc caggcgcagc gcctcctcca ccgcctcgtg aacgaaccgc
     6301 ttcatcagct cctcgcccca cggctggagc gcgcgcgggc cgaagccgcc gccgaagccc
     6361 ttcgccccgg cgaagcccac gccgccgatc tcgacggcct cgccatcgag catgtggacg
     6421 ccggcatcgg tcaggatcgc cttgaccgtg tccggcttgc cggcctcgta atcgtggttg
     6481 ccgagcacgg ccaggatcgg gatgcgtacg gccgtgcgca gggtctgtgc cagggcgcgg
     6541 gcctcgtcct cttggccgcg gtcggtgagg tcaccgcaga gcagcagggc gtcggcgccg
     6601 tcgccgatgc tcgtcaggta ccgcgcgagc aggtcggcag gcagccggga gcagtgcagg
     6661 tcagcgatcg ccgccagccg cattcttcgg ctgaccgtcc tcgatcgccg aggtccagac
     6721 ggcgacgtcg tcctcggtca tggtcccgtg ctcgagccgg ccatcgcgat agccccacgt
     6781 ctcgacgtcc atgaggtact gggcgcgcga cagcagcgtg ccgaagcaga cgggtgccgc
     6841 gctcaccggt cgcccggcgt cccggccgag ccgccggctc agctcggcca tgacctgccg
     6901 gggaatccgt ccgttctcga tggggtagac gaagccgaag agcaccaggt gggcgaggag
     6961 gactcgccag tgggggccga agcggcggag gaggcgccgc cagtcgagac cgccggcgca
     7021 ggcgcggagc aggtgggcga cgtccgcgcc gtcgtagcgc tcgcgctcca tgatgaaggc
     7081 cttggaccag atcgtctcct cgggcggaca gaggcgcacg ggcacgccga gcacggcctc
     7141 gtccaccgcg tgggcgaacc actcgtcgtc gacgcccgcg cagccgttgc ccgagccgaa
     7201 gatgacgtcg accacgtcgt cgccgtcgta ggccttgccg agccagtggg ggaacgccat
     7261 ctccgtccgg tacccggcgg ccgcgagcgc gccgagagcc cggtcgaagt cctccctccg
     7321 gacgaagacg tccaggtcct tggtgtcgcg cgcgatgccg gtgtagcgct cgaacgagta
     7381 ggcgccgccc acgaggaact ggacgtcccc gtcggtcagc gcgtggagcg cggcgacgta
     7441 gaagcgagcg gcttgcggac gaccttcggc cacaggcacg ttggtggacg atgcagatcc
     7501 cgtgccggtg ggtgtgaatc aggggggagc gacggacgtg tcggcgacgc ccaccgtgct
     7561 gctcaccgcg atcacggatc cgagcagtcc cgcgcgaggc cgcagttccg gcacaggatc
     7621 ttgcacttct gctcctccat cctcgcgccg cagcgctcgc agaagtggac ccactcgatg
     7681 accccgccgc cgcggtcgat cgccccgtcg tccacgaccg tcagcgtcgc accgccaccg
     7741 gagcggagtc aacgtctgat cgcggtgacg ccgcgcggag ccgaggcccc tcgcacggcg
     7801 ggcaccggtt ccgtacgttc cgcacccccg acgtctggtg ccgtacactg gagcggatga
     7861 gcgaggcctg ggaagacggg gagcggctac gccacatctt cgacgagacg cgggtggtgc
     7921 gccggccgct ccgggggatc atcgcgggct accacgtgct gccctacatc ctcgtcggcc
     7981 ccgagcgcga ccagcctggc cgttcggtgg aggtgcgcgg gcggatcaag gtctcgccgc
     8041 gcctggtgat cacgccgggc cgtgacggcc ccacctacgg ggagctcttc ggcgagcgcg
     8101 agctgatgga ccacacgctg ctggcgcgtg tcttctcgtt ccgctacgcc cagcgcgtct
     8161 ccctcgagag cgacgagctc acgatccgca agcagacccg cgaccccgag gggcatctcg
     8221 agcgggtgct ggaggaactg gcgcagcgcg aggtgatcga caccggcgtc atcgtgtcgc
     8281 cggatccgcg gttctacccg gtgtcgatcg accggttcat ccgcgagatc ctcgacgagg
     8341 agttccgcga gtagactccc gctacacgac cttccgcgca tggaggtcgg cgatgaccgc
     8401 cggcccgtag cccagctcgc gcaggatctc gtcggtgtgc tggcccaggg cgggcgccgg
     8461 ccgccggacg gcgcccggcg tcgccgagag cttcacgggt acgcccagca cgcggccctt
     8521 ccccgccgtc gggtgctcga cctcgacggc catcgcgcgg tgccgcacgt gggggtccgc
     8581 gaacacctcg tcgtacccga gaatcggccc cgcgggcacc cccgcgccct ccagccgcgc
     8641 gagccacacg gccgtcgtgg ctgtccggaa gcgcgcctcg agcagcgcct ccagctcggc
     8701 ccggtgtttc accctgagcg cgggcgtcgt gaaccgcggg tcctcgagca gcggctccac
     8761 gccgagcacg gcgcagatgc gcggccagaa gccgggggcg ccgccgccga gattgagcca
     8821 cccgtcggcg gtgcggaacg cctggtacgg cgcgttcgtg cggtgcccgg gtccgaggcg
     8881 gcgcggcggc tcgccggtcg cgaaatacgc cgacgactcg tacacggacc aggcgatcgg
     8941 tgtctcgagg agcgaggcgt cgatcgcctg cccctcgccg gtccggacgc gcgcctggag
     9001 cgcggcgagg atggcgatcg ccgcgaacat gcccgtgccg atgtcggaga tggggacggg
     9061 cacgggcagc ggcggccccg tctcgtcccc gttgatcgcc atgatgccgg acatgccctg
     9121 ggcgatccgg tcgaagccgc cgcgggcggc gtaggggccc gtctggccga aacccgagat
     9181 ggagcagtag acggtgcgcg ggttcagcgc gtggacgtct tcccatccga ggcccaggcg
     9241 cgccgccgtg cccggccgcc agttctcgac caccacgtcg gcgcgagcgg cgaggcggcg
     9301 cgcgacggcg aggccgtcgg gcgccttgag gtcgagggtg agcgagcgct tgttgcgatt
     9361 gaccgtcatg aacggcgccg actcgccgcg gacgtgcggg ccgctggcgc gcatgtcgtc
     9421 gccgttgtcg ggccgctcca ccttgacgac gtcggccccc agatcggcca ggatcatggt
     9481 gcagtacggt ccggagagct ggttcgtgag gtcgaggacg aacacgccgt cgagggcacc
     9541 cgggtgcacg ggaacggagt ctaccgcaga ggcgtttggt gagtgggcaa ccttgacact
     9601 gtggggcgcg aatgctatat atgctgcgca cttatcgagt cctggaggaa actctcatgc
     9661 gacttcgtgg actgggagcg ctcgtctgcg ccgtggccgt cctggccggt ggccttcccc
     9721 tggtggaggc gcaggcgccg atccggatcg gcgcctccct gtcgctgacc ggtacctacg
     9781 ccaagctcgg caactaccag aacgagggct acaagctctg cctcaagcag gtgaacgaca
     9841 agggcggcct gctcggccgc agggtggagc tggtgatgta cgacgaccag tcgacaccgg
     9901 cgaccggcgt acgcctctac gagaagctga tcaccgagga caaggtcgac gcggtgatcg
     9961 ggccctactc gtcgccgatc accgaggcgg tcgccaacgt caccgagaag tacaagaagg
    10021 tgatggtcac cccgctcgcg gccaccacgt cgatcttcaa gaagggccgc aagcacatct
    10081 tcatgatcat ctctcccgcc gaggtctacc tcgagggcct cgtcgacatg gcggccaagc
    10141 gggggctcaa gaccgtggcg gtcgtcaacg aggacacgct cttctccaag gccgccgcgg
    10201 ccggcgccgc agacctggcc aggaagcggg gcatgcaggt cgtcttccag gaggcctatc
    10261 cgaaaggcaa caccgacttc tcggcgctcc tcaccaaggt gcgcgccgtg aaccccgacg
    10321 tgatcgccgc ggcgacgtac ttcgacgacg cggtggcgct cacccggcaa atgcgcgagc
    10381 tgaacgtcgc gcccaagatg tacggcgtca ccgtgggcgg cgacctgccg gagttccacg
    10441 acctgctcaa gcagaacgcg gactacatct acggcgccac ccagtgggag cccacgctgc
    10501 cgtatcccgg caacagggag ttcttcgagg cctacaagaa ggagttcaat cgggagcccg
    10561 tctatcactc ggccgcgggg tacggtggct gcctgatcta cgcggaggcg gtgaagcgcg
    10621 cgggttcgct ggactccgac aaggtgcgcg agcagctcct caagctggag acgcgcacgg
    10681 cgttcggcgg ctacaaggtc gaccaggacg gcttccagat cgcgcacaag atggtgacgt
    10741 tccagtggca ggacggcaag aaggtcaccg tctggccgga cgagctcgcg caggccaagg
    10801 cccggttccc cacgccggcc tggaaccagc gataggcgcc ccggaggggg gcctcgctcc
    10861 cctccgctcc ccttccccca ggatcgaggg cgccggcgac gccggcgccc ggacagggcc
    10921 ggccatgcgc ctgacggcgg cgacgaccgc ggcatgaaca tcgtcatcac accggcgatg
    10981 ctggggcagg tcatcgtctc cggcatcctc gccggcagcc tctacgcgct ggtctctctg
    11041 gggctcgccc tcatctacgg cgtcatgcgc gtcatcaaca tcgcccacgg gccgctgctc
    11101 atgctcggcg catacacgac ctacttcctc gtcgccggca ccggaatgaa cccgttcctc
    11161 acgattcccg tggcgatggc ggcgctgttc gtcgtgggcg tgctcctcca gcgcaccctg
    11221 gtattccggg tcgtcgacgc gccggagctg tcgtcgctcc tgctgacgtt cggcatctcg
    11281 atcgcgatcg tgaacctcgc ccagctcgtc ttcagctccg acctgcgcgc cgtcgagtac
    11341 atcaccggct cgtgggtgat ggcggggctg gccttctcga agtcgcgcgt catcgccttc
    11401 gcgttcgcgg cggcggtgac ggccctggcc ttcctcttct tgaaatccac ccggctcggc
    11461 aaggccatcc gcgcgacgtc gcagagccgc gaggtggcga tggtctgcgg catcgacgtg
    11521 cggcgcatcc acctgctcac cttcggcttc gcctcggcgc tggccgcggc cggcggcgcg
    11581 ctgctggcgg tgatcgtcgc gatccagccc gagatgggcg gcgtgtggac gttcaagtcg
    11641 ttcctcgtca tcgtgctcgg gggcgccggc aactacccgg gcgcgctgct cggcggcatg
    11701 ctcctcgggc tcatcgagca gctcgcctcg ctgttcctca ccacgcagct ctcggaggtc
    11761 gtcgcctacg tgctgctggt cctcgtgctt ctcgtccggc ccacgggcct gctcggcggg
    11821 cggcagacgt gaagcgcgcg ttcctcgccg cgctcgtcgt cgcgctggcg gcgctggccg
    11881 tgtaccccgc gttcggcacc gggtacggcg tgcgttcgat gctgcagatc ttcatgtgga
    11941 tcgcgctggc cgggtcctgg aacctgatct cggggctgac cgggtacgtg tccttcggcc
    12001 acgtcgcgtt cttcggcgcc ggcgcctaca ccggcgcgat cctcgtcacg cagcacggct
    12061 ggccgtggcc ggtggccgcg ctggccggcg gcctcgccgc cctcgtgctc gccgtgctga
    12121 tcggctggcc gtgtctgagg ctcaaggggc cgtacttcgc catcgcgatg ctgggcctga
    12181 acgaggtgct gcgcgcgctc gtgtcgtact tcgagggcgt caccggcggc ggcaacgggt
    12241 tgtcgctgcc gacgctcgac gcgagcgtgc ccatctacta cgtcatgggc ctcctcgccg
    12301 tcgcggtgac cggtgtcacc tccctcatca tcacgtcgcg cttcggcctg cggctcatga
    12361 cgatccgcga ggacgagctg gccgccgagg ccatgggcat cgacacgttc cgccacaagc
    12421 tctacgcgtt cctcctgtcg gcggcgggtc cgggcgtggc cggcgccctc accgcgcgcg
    12481 accagggcta catcgagccc atcagcgtct ttcccctggc gaccacggtg acgatgatcg
    12541 tcatggtgct cttcggcggg aaaggcacgg tgtggggacc ggtgctgggc gcggtcgtgc
    12601 tcttcgtggc ccaggagatc gtgtgggcgc gatacccgta cgtccacccg ctgctcttcg
    12661 gggccatcat cgtcgcggtg gtgctggcga tgcctcgcgg cgtgctcggg ctcctgcagc
    12721 tgaagtaccg tctaccccgg acgatctgat gtccccgccc gcgctcgagg tccaggacgt
    12781 gcgcaagagc ttcggcggcg tcaccgcggt caacggcgtg tcgctccgtc tcgatcccgc
    12841 gcgcatctac gggctcatcg gccccaacgg ctcgggcaag accacgctct tcaactgcat
    12901 caccggcgtc gaacgccggg acgccggcca cgtgcggctc cacggtgagc ggatcgacgg
    12961 gctccggccc taccagatcg cgcagaaggg cgtcgggcgg acgttccagg tgatccgcgt
    13021 gtttcccgag ctcaccgccc tcgagaacct gctcgtggtc acccggggcg accgggaggc
    13081 cgcggtgagg cgggcgctgg agctgctggg cttcgtcaag ctcgagcggc tccgggacga
    13141 gtacgcaggc aacctgtcct atggccagca gaagctcgtg gagttcgtgc gcgtgctcat
    13201 gcgcgatccc tccttgatcc tgctggacga gccagcggcc ggtgtcaacc gcacgctgct
    13261 caacgacctc ctcggggccg tgcgccagct ccgggaccag ggcaagaccg tgctcctcgt
    13321 cgagcacgac atgaaggtcg tcatggggct ctgcgagacg gtgttcgtcc tcgatcacgg
    13381 cgagaagatc gccgaggggc cgccgggcgt catccagtcg gacgagcgcg tcattgaagc
    13441 gtacttcggc cggtagcctc gcggacgcat gccgccgcgc ggtcgcggcc ggcttgctgc
    13501 tggggctggc cgcgacggtg ctgcggatgg cgtggcggtc gcgcggctgg ccgctcgtgc
    13561 acgatgcgcc gatcatgcac tacgtcgcct ggcggatcgg tgagggggcc gcgccgtacc
    13621 gggatctgtt cgacatgaac tttccgggca cgtacctgct tcatctcgcc gtcgtgcggc
    13681 tgctggggcc aggagacgcg gcgtggcgcg ggttcgacct ggcgtggctc gcggccggcg
    13741 cggtggccgt cgccgcgttc gcggcgccct ggggccgggc cgcggccggg ggcggcgcgc
    13801 tcttcttcgc ggcgcaccac ctcgcgggcg gcgccgggca ggcgggtcag cgggacttcc
    13861 tgctgtgccc gttcctcctg ctcggcgccc tcggcgtggc gcgctggctc gagcagcggc
    13921 gtgccagcag cctcgcctgg ggtggcctcg tgctcggcgc cggcgtgacg atcaagccgc
    13981 acgcggcgct gctggccggt acgctggccg tcgtgatcgc gctcggcgcg tggcggagcg
    14041 gcagcggagt ccccgtggcc gcgttcggcg gcgcgaccgc gctggcgcct ctggccgtcg
    14101 tggcctggat cgccgctgtc ggcgcgctgc ccgcgtggcg ctccatcgtg ttcgaatacc
    14161 tcctgccgct ctattcgcag ctggcccgtc cagaatactg gggcttccac cgctggcacg
    14221 tctggatccc gatcgccctt gccgtcggcg cgagcctgac gagcgcgctc gtctcccggc
    14281 ggttcggtgc gcgccacggc gtcgccgtgc tggggctcgt ctacggcgtg gcgcactatg
    14341 tcggccaggg caagggctgg gagtaccaca tctaccccgc ggcggcgttc gcggccgtgc
    14401 tgctcttctg cgagctggaa gctctcctgc gcgcgcgccg gcccgtcgct gccatcgcgc
    14461 tcgccgcgtg ccttctcgcg acagtcgtca tgctggacac caaggccgcc gaggctgccg
    14521 ggtcggccga gacgggctgg atcgcccgca aggcccggcg ggtcgtggcg ctggcaacgg
    14581 acctccgcgc gcggctggca ccgggagacc tcgtccaggt gctcgacacg acgggcggtg
    14641 ggatccacgc gctgctgcgc ctccacgccc ggcagcccac gcggttcctc tacgacttcc
    14701 atttcttcca cgatgccgac gcgccggtga tccgcgcgct gcgagacgag ctggtgcgtg
    14761 gcctcgacgc ccggccgccc cgcttcgtcg tattcttccg cgagggctgg ccggccggcg
    14821 gcgacgagcg aatcgcggcg ttccccgagc tcgccacacg gctggaccgc ggcttcaggc
    14881 tcgacacgcg gggtgacggc tacgtgatct atgcgaagcg agacgatccg tagcatcatc
    14941 cgcgcctacg acgatcccgt cgtccaggcc tactgctggg tgcggttctg gatcctgcgc
    15001 cagcggttcc tcgacgagat cgggcagtat ctcccggagg ccgggcccgt gctcgacgtc
    15061 gggtgcggct tcgggctgtt ctccctctac tacgcggcga cggcgccggg ccgcgtcgtg
    15121 cgtgggctcg atctgaacgg gcggcgaatc gcgctcgccc gccgcgccgc ggcacggctc
    15181 ggcctcgaga acgtggcgta cgaggaggcc gacgcccggc actacaaggg cgacacggag
    15241 gtcgcggcgg cgtacatgct cgacatcgtc caccatgtcc cgcgcgaatc ggtgccgccg
    15301 ctgctcgccc agctccggcg gagcctgccc gccggcgggc tgctgctcgt caaggacgtg
    15361 gacacccggc cggcgccgaa gcgctggttc acgtgggcgc tcgaccggct gatgagccca
    15421 cgcacgcccg tggcctactg gagcgccgag gccctcaccg aggcgctcga ggccgcgcgc
    15481 ttccgcgtgc gtcgccacct gatggtggac gtgctcccgt acccccacgt cctctacgtc
    15541 tgcgaggcgc ggccgtgagc gccggcacca cgctactgac cgcggagggc atcaccgccg
    15601 ggtacggcaa gatggacatc ctgcacggag tcgcgctgcg cgtggacacc ggcgagatcg
    15661 tcagcgtgat cggtcccaac ggcgccggca agtccacggc gttcaagacg atcgtcgggc
    15721 tgctccaccc ccgcgagggc cggatccgct tcaacggcga ggacatcacc ggccttcgcc
    15781 ccgatctcgt cctgcgccgt gggctcgcct acgtgccgca ggggcgcatc gtgttccccc
    15841 agatgaccgt gctcgagaac ctcgagatgg gggcctacat cgagcgcgat ccgaagcgga
    15901 tcgtccaggc gctcgagcgc gtctacgccc tcttcccggt gctggcagag cgcaagcgcc
    15961 agcgggcggg gacgatgtcc ggcggcgagc agcagatggt tgccatcgcg cgcgcgctca
    16021 tgaccacgcc cgcgctcatc ctgctcgacg agccgtcgct cgggctgtcg ccgaagttcg
    16081 tcacgctgat cttcgacaag ctcgtggaga tgaagcgcgc cggctacacc ctgatgctcg
    16141 tcgagcagaa cgccgcgcgc gcgctcgcca tcgccgaccg cggctacgtc ctggagctgg
    16201 ggcgcaaccg gttcgagggg acggggccgc ggctgctcgc cgacgcggag gtcaagcgcc
    16261 tgtacctcgg aggatgagcg cgcgcgtctc cgtcctcatg tgcgtgcaca acgggctcgc
    16321 gctgacccgc gcctgcctcg acagcctgtg cgcggcgacg gagcccttcg agctggtcgt
    16381 ggtggacaac gcgtccaccg accagacgcc ggcgttcttc gcgcgcgcgt cgtatccgtt
    16441 cccgatccgc taccggcggg aggagcgcaa cgaggccgtg atcgcgcgcc tcaacaccgc
    16501 gtggcggctg gcgaccaccg atcacctctg cttcctgcac aacgacaccg agatgatcga
    16561 gcccgcgtgg ctggcgcggc tgctggcggc gctggccgag ccgcgggccg gtctcgcggg
    16621 tctctacggc gccaagcgcc tgcgtcgcga cggccgtctg atggggcgca ccatcgtcca
    16681 cgccctcgcc gagggaccga ccgtccggcc gccctgggaa gaggtcgcct tcgtggactc
    16741 ggtctgcatg tgtctccgcc gcgacgtgct cgaggcggtc ggcggattcg acgagggtta
    16801 cggcttctac cacggccacg accgggacct gtcgctcgcg gtgcgcgaga ccggacgccg
    16861 ctgcctcgtc gcctacgcgc ccttccacca ccggggcggc ggcacccgcg cacacgagtt
    16921 cggcgcgcgc cccgaccgcg agcgggccga cctggacctc cggaccgccg caatggcgcg
    16981 cttcgcacgg aagtgggcgc accggctccc ctgcgacgtg cggtcgctcc gcgagcgcgt
    17041 tcgcgactgg gtgcggacga ccgcgcgagg ccgggtctcc cgcgccgggg atccgttcgt
    17101 gcggcgttgg acggggtgag tgtgagatgg gtgggcacgg tttccgacga cgtgcagaac
    17161 gtctgactcg cgaggccagg cggtcaccgt ccggcgagcg cctttcgtac cgcggactcg
    17221 atctggtcga tctcgaacgg cttgaggacg agctgatcga tgccgccggc ggccagatcc
    17281 gcgctggaga gctgcgcccc ccagccggtc accagcacga tacgcgctcc ggggcgcacg
    17341 cggcggacgt ggcgcgcgac gtcccagccg ctggcacccg gcatgccgag gtcggtcacg
    17401 acgacgtcga agggctccgc gtccagccgg gcgatcgccg tggtgccgtc gccgcacgcg
    17461 gtcacccggc agccgagggc cgtgagcgcg tcctccagga cgtggcgcac cgcctcctcg
    17521 tcgtccacga cgagcgcgtg cgtgccggcg agcggctcgg ccgagggcgg gacggtctcc
    17581 tccacgccgg acgccgcgat gtccggcgtc gccacgggaa ggcggatgct gaccgccgtg
    17641 ccctcacccg ggcggctgtc cacatcgacc tcgccgccgt ggcgcatgag gattccgtag
    17701 gcgacgctga gcccgaggcc gctgccacgc gcccctttcg tggtgaagaa cggctcgaac
    17761 acgcgctcgc ggacttcgtc ggtcatgccg acgccggaat cggtcacggt gcaccgtacg
    17821 cgatcgctct ccggcacggt cgtgagcgtg aggcgcccgc cggcgggcat cgcgtcgagc
    17881 gcgttgagca ccacggccat cagcacctcg cgcagctcca gcgggtcgcc ggccaccaga
    17941 tccgcgggtg tggcgtccac gcgcacgtcg taggcgatcc cgcgcgccgc ggcctcgtcc
    18001 ctccagcgtg gccgggtcgc gtccacgacc tcctgcgcca gggcgcccag ctccaccgtg
    18061 agaaacggcc gctcgcgccg catccgcacg aactcctgaa cccgccggag cgtgcgcgct
    18121 gcctccagcg cggcctcctc gacgacctcg agctgccgcc gcacgtccgg gtcttcgacc
    18181 tgggcgagca ggagctgggc acggccgagg atcgcggcga gcgtgttgtt gaagtcgtgc
    18241 gagaggccgc cggccatgtc cccgagcgcg cgcagacgct cggcgcgcac gacgccgcgc
    18301 tgggaggcgg caagctgccc cagcgccgct tgcagctcgg cgtagagccg cgcattctcg
    18361 atggccacgg ccgcctggga cgcgaccgag gtcgccagct cctcgaagcg cgccggaaac
    18421 ggccgcgcct cgcgttcgat gtgctcgcgc gaggtgaagc ggacggcagg gtccgctttg
    18481 cagttgatga gctgcatgac cccgatcacg cgggcggccg gcgtcttcag cgggaccacc
    18541 agcatcgact tcgtgcggta cccggtctcc gtgtcgtaga cagggtccac gaggaacgcg
    18601 gagccggcgg gcggcgtgta ggcgtcgggc aggttctgcg gctgcccggt gaaggcgacg
    18661 tggccggcga cgctcgtggg gccgatggcc agcgtgcccc cctcgaacgg tgcctcggcg
    18721 ctgtcgttct gcacgaccgt gaaacggagc cgtcggcgtc cggcggcgtc ctcctcgacc
    18781 aggtagagcg agcccgcgtc ggcgtgcgtc agctcacggg cgcgggcgac gacgagccgg
    18841 agcagggccg tcgggtcgcg ctcggacgag agcgcgatgc cgatggcgtt gaggctcgag
    18901 aactccgtgg ccagccgctc gaggttcacc ctcatctggg cctgctccag gtgtagctgg
    18961 gcgtgcacgg cggcgttgtg cagcgtctgg accaggatgg acatgggcag cgtggacagg
    19021 aggtacgcgt ggacgtgctc ggacggggtg aggggcaggt gggcggcatc gcccagcacg
    19081 acgatggcaa tggcagggtc ggcgcccttg agggccaggg cgcgcacgag ctgggcgacc
    19141 tgggcatcca ccagaatcac ggcgggggcg gtcacgacgc cgggctcggc cctctcgtcg
    19201 agcacggtac ggcgacactc ctgcagccgt tcggcgagac gggcagcctg cccgccggcg
    19261 aagtggaaga gacggtagct cacgggccct cgaccgcccg caaactgtct gccgggcgtg
    19321 gctgcttgtc aagcgggggt cgcggcgggc cgcgaccccc gtcaaggagt ggtggagctg
    19381 aggggattcg aacccctgac cccaagactg ccagccttgt gctctcccaa ctgagctaca
    19441 gccccacgcg agggaccaac ttacaggccg ggtccggggg tgtcaagaaa ggtggtcgtg
    19501 agcggtgaag gtggtgccgg tgaggggatt cgaaccccta cgcccttgcg ggcttcggat
    19561 tttgagtccg aattgtcttc cagttccagc acaccggcac gttcgtcctc gatgcgcgtc
    19621 ctcgaccgag gcctcattgt acagccatcc gccccgtggc cggccgatcg gccgtttcgg
    19681 cgcttgtccg agtggccgaa gaaaccgtac cgcaatcact cgatttgcgg cgatttctgc
    19741 cttcggatcg ttccactccg gtggccccac gacgaccgat cagccgctga ggacgtcggc
    19801 ccgaccctcc cagcgcaacc acccagcccc tgtgccacaa tagacccgtc gcgggccggc
    19861 cggacgcgcg gccgacgcga cacgaccaga tgggtgagcg caccttcgcc gaagacgtca
    19921 agcagctcgg ctacggcgcg ggccaggtcc tccgcggcga aggcgtcctt gcgatcacca
    19981 aggccctgct ccagtccggc gtcagctacg tcgggggcta cccgggcgcc ccggtgtcgc
    20041 atctgctcga cgtcatggcc gacgccaacg agccgctgct caagccgatg ggcatctact
    20101 tcgagcagtc gggcagcgag gccgcggcgg cggcgtcgct cggcgcctcc atcaactatc
    20161 cgatgcgcgg cgccgtcacg tggaagtcgg tggtcggcac caacgtcgca tccgacgcgc
    20221 tctcgcacgt cgcctcggcg ggagtcatcg gcggcgcgct gatcgtgatc ggcgaggact
    20281 atggtgaggg cgccagcatc ctccaggagc gtacgcattc cgctgcgctc aagtcgagcg
    20341 tgccgctgat cgatccgaag aacagcctgc cctccttcgc gcgcttcgtg gaggagggct
    20401 tcggcctcag cgaggcctgc cacgagccgg tgttcttcag catccgcatc cgcgcctgtc
    20461 acatg
//
LOCUS       DATIAZ010000250        18097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_331860, whole genome shotgun sequence.
ACCESSION   DATIAZ010000250 DATIAZ010000000
VERSION     DATIAZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 18097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18097
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331860"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..2518)
                     /gene="leuS"
                     /locus_tag="VLF19_08950"
     CDS             complement(32..2518)
                     /gene="leuS"
                     /locus_tag="VLF19_08950"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSE93419.1"
                     /translation="MSSDRYPVHEIEAKWQRAWQERRAFRAVEDPGRPKFYCLEMFPY
                     PSGRIHMGHVRVYAIGDLLARYKRMRGFNVIHPMGWDAFGLPAENAAIEHGVHPAIWT
                     YENIDNMRAQLARMGISYDWEREVTTCDPSYYRWEQLIFIRMFERGLAYRRRSTVNWC
                     PSCQTVLANEQVEAGRCWRCDTEVTPRDIDGWFFKITAYADELLAWCDRLPGWPERVL
                     TMQRNWIGRSEGAEFDLAVAGRPGLSLRVFTTRPDTGFGMTYAVLAPEHPLVERLLAD
                     DAERARVAAFRAEVARQTEIERLAADRPKRGLRLGARVVNPFNAAEIPLYIADYVVMG
                     NGTGAIMAVPGEDQRDWDFARQHRLPIVETVKRPEGWVGEAYAGDGIKINSGFLDGLT
                     VADARRKAIDWLVQRGLGEGRVNYRLRDWGISRQRYWGAPIPILYCDRCGMVPEAEEN
                     LPVVLPRDVQISGKGGSPLADVATFVNARCPRCGGRARRETDTMDTFVESSWYFLRYC
                     SPHYEGGMFDPAAADYWMPVDQYIGGIEHAVLHLLYARFYTKVLRDLGLTKVDEPFMA
                     LLSQGMVIKDGAKMSKSKGNIVDPDEQIRKYGADTARLFSLFAAPPEKDLDWNDHGVE
                     GAFRFLNRVWRFVIAHLDELASGPAVAAATLSPDGRAFRRTIHETIRRVTGDIERDFH
                     FNTAVSAIMELVNALQAFEASTLDRLPAGERRALMCEAVDTLVLLLAPFCPHVTEELW
                     ERLGRAGGVVAQRWPEADPAALVKDEVTVVVQVDGKVRSRLTVDMGASEAEVRRQALE
                     DDKVRPWLGARTIDRVVVVPNRLVNIVTRS"
     gene            complement(2605..2871)
                     /locus_tag="VLF19_08955"
     CDS             complement(2605..2871)
                     /locus_tag="VLF19_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93420.1"
                     /translation="MRKLMAVIAVAVALAFGASLVEAGQCPLLIKQLKDGVAKVSDTK
                     KKASVEKMIAEAEKLHADGKHAESVKKCEEAAKAAGIKLEMKKS"
     gene            complement(2868..4781)
                     /gene="uvrC"
                     /locus_tag="VLF19_08960"
     CDS             complement(2868..4781)
                     /gene="uvrC"
                     /locus_tag="VLF19_08960"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSE93421.1"
                     /translation="MTLEEKLQTLPERPGVYLYKDARGQVLYVGKAASLRGRVRSYFQ
                     ESRPRDAKTDALVRQVADLEYVVTDNELEALMLEVNLVRKHRPRYNIILRDDKHYPFL
                     RLTTNEEYPRLLVARKVQRDGAQYYGPFYPATAMRDTLRLVRQLFPLRTCAIKIDGRL
                     ERPCIQYFIHRCNAPCTGWETHEGYARTVREVQDFLDGRDDDLAVRLTREMERAAGEE
                     KFERAGVLRDQIQSLNKVRERQKIISTDLVDQDVLGVVRQGADACVELFFVRKGRLVG
                     QEAFFFDRVSGWMDGEVLSAFVRQFYGKQVTPASEILLSEEVPEADLTTEWLSGLAGR
                     KVALVVPQRGSKREFVAMAEANAAIALRHHLLSRENRQQAILEELQRALELPGLPNRI
                     EGYDISSIHGSEQVGAMVVWENGDMKKDDYKRFRIKTVAGADDYASLAEMLRRRFARS
                     LEQGEALPDLVLIDGGRGQLNAGLTVLAELGLDWMPVAALAKQREEVYRGESMEPLLL
                     DPTSPALHTLQKIRDEAHRFAVTYHKKLRTRRTIQSALDGIPGVGPTIRTSLLKMLGS
                     ARRVRESSVAELAAVPKVTPKLAQRIYDHFHAGSGAVGGGERAPAAAGNRAGAARVVA
                     DERQPGAEGGDSP"
     gene            complement(5016..6998)
                     /gene="uvrB"
                     /locus_tag="VLF19_08965"
     CDS             complement(5016..6998)
                     /gene="uvrB"
                     /locus_tag="VLF19_08965"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HSE93422.1"
                     /translation="MFRLTSEFPPRGDQPQAIARLTELIRGGRRHCVLRGVTGSGKTY
                     TMANVIANAGRPTLVLSPNKTLAAQLFSEFKAFFPENAVEYFVSYYDYYQPEAYIPQS
                     DTYIEKDALVNDEIDRMRHSATRSLMERRDVVVVASVSCIYGIGAPETYQEMHLALRE
                     GEQIDRDEIIRRLIAVQYERNDYDFHRGTFRVRGDIVEVFPANEETHALRIELFGDVV
                     DAIHRIDPLRGQVQERLPDVHVYPASHYVTPAAQLERALGTIHEELGERLAFLRQRNR
                     LLEAQRLEQRTLFDMEMLRELGFCHGIENYSRHLTGRRPGEPPPVLIDYLPKDALVII
                     DESHVGVPQIRGMYYGDRSRKEALVEYGFRLPSAFDNRPLTFEEFSRLTGQTVYVSAT
                     PGPYELELAGDALAEQVIRPTGLMDPEIMVRPATEQVDDLLHEVRVRAERAERVLVTT
                     LTKRMAEDLTEYYQQAGLRVRYLHSDIDTLDRVAVIRDLRLGKFDCLIGINLLREGLD
                     IPEVALVAILDADKEGYLRSETSLIQTAGRAARNVNGKVIMYADHVTQSMAATIRETE
                     RRRTLQAGYNAEHGITPESIRTSIRELLQTIYERDYYTVEVAEPGPETFESPADLERR
                     IAELDGKMKEAARRLDFEQAAELRDRVRALRKLQTV"
     gene            complement(7135..7665)
                     /locus_tag="VLF19_08970"
     CDS             complement(7135..7665)
                     /locus_tag="VLF19_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSE93423.1"
                     /translation="MDEIAVLVLNYTYEPLHFTNARRAITLLIAGKAEAVEGSPRVVR
                     SPSRAFALPAVIRLAVYIRKPFLDRVAFNKKNILRRDGYTCQYCNRRGERLTVDHVMP
                     RSRGGETSWTNVVAACLRCNLKKSNRLLDEVGMRLLREPVHPKFVFSTHMLKHPHAHS
                     LLDSWRKYLLAVPTST"
     gene            complement(7717..8652)
                     /locus_tag="VLF19_08975"
     CDS             complement(7717..8652)
                     /locus_tag="VLF19_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HSE93424.1"
                     /translation="MAMISRALLLALSTRPRLGQWMDRLPLTRRFVRRFVAGTTADEA
                     LAVVADLNARGLLAAVTYLGENVRTAAEAVHATDTYLALLDEIKRRNLTALPSLKLTH
                     LGLDLGEPVCVANLTRVLERGRAVGTRVWIDMESAAYTDRTLDLYARLRPAHPHAACV
                     VQAYLRRTPVDVERLIDLGAAVRLCKGAYREPPRIAHPDKADVDRAYDALVDRLLAPD
                     ALARSVYPGFATHDERRIAHVRERAGALRVRRERFEIQMLYGIRSELHATLSAQGLAV
                     RVLVPFGEDWYGYFMRRLAERPANVAFVLRNLVRR"
     gene            complement(8643..9581)
                     /locus_tag="VLF19_08980"
     CDS             complement(8643..9581)
                     /locus_tag="VLF19_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HSE93425.1"
                     /translation="MTSPRLAGIDLGSNTVRLLVVEVGDAGGWRVVDQDQRVTRLGEG
                     LAASGRLGETPMARTAATIVEYAGRAVRAGAREVRVVATSAVREAVNGGDFVTALARA
                     AGVRVDVVSGEDEARLTVRGIAAGLGRGAGSMLAFDIGGGSTEFTLARDGQVTATVSL
                     GLGVVPLAERYPFPGAVDAGRYGALEREIRQRLATELPVTIRCSGLADLVGTAGTVTT
                     LAALDLGLAAYDAARVQGHRLARPAVERLRDRLSALTVRERGDLPCLEPGRADLIVPG
                     VAIVLAALDATGTAGVVVSDWGLREGIMAEAVERWR"
     gene            complement(9613..10011)
                     /locus_tag="VLF19_08985"
     CDS             complement(9613..10011)
                     /locus_tag="VLF19_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSE93426.1"
                     /translation="MSRLGRCGAIALAAVVLVAGAGVAAVSAQGEWKAPADAKNMKNP
                     VKGVGNAKKNIETNCVTCHGASGKGDGVAAAALPPPKPADWTSARVQKETDGELFWKI
                     SNGRGAMPPWKHLPEKDRWEIVNYIRTLKK"
     gene            complement(10094..11500)
                     /locus_tag="VLF19_08990"
     CDS             complement(10094..11500)
                     /locus_tag="VLF19_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSE93427.1"
                     /translation="MTERPGTTERPLRVAIVGAGPAGYYAADHLLRQDGAVVEVDVFD
                     RLPTPYGLVRAGVAPDHQKIKAVTAVFDKVAAHPRFRFYGGVELGKDVSVEDLRRHYH
                     QILYSTGAQTDRRMGIPGEDLRRSHPATEFVAWYNGHPDYRDLTFDLSQERAAIVGVG
                     NVAIDVARILSRTAEELERTDIADHALEALRASRIREVVLLGRRGPAQAAFTNPELRE
                     LGELAAADVVVRPDEVELDELSRAALEKSGDRAERKKVEILQEYARRPPTGKPRRLAL
                     RFLVSPIEVLGNDAGQVTGLTLVRNQLVATETGSLQAKPTGQVETLPAGLVFRSVGYR
                     GVPLPGVPFNESWGVILNEKGRVLDPDTRRPRLGEYTAGWIKRGPTGVIGTNKPDAAE
                     TGAAMLEDLAAGHVLDPAEPGAAAADRLVRSRQARVVSYVDWRRLDALETARGRVMGR
                     PRVKFTRLAEIHAALGRE"
     gene            complement(11497..11931)
                     /locus_tag="VLF19_08995"
     CDS             complement(11497..11931)
                     /locus_tag="VLF19_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSE93428.1"
                     /translation="MNIASVLASKGVEVVTVRPEQTIREALALLAERNIGAVVVATGE
                     GRPAGIVSERDVVRTLARDERVFDWPVSRIMTRDVVVGAPADDLATVGSTMTQRRIRH
                     LPVMDGGKLVGIVSIGDIVKAQRDRYQGEVDTLQVQLLKDRA"
     gene            complement(11995..13374)
                     /locus_tag="VLF19_09000"
     CDS             complement(11995..13374)
                     /locus_tag="VLF19_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE93429.1"
                     /translation="MVPGTALGVLVGDLAYTWLAVRLMRHTGRADVTAMPFGIDTPTL
                     FAMVFGVLGPVKMATGDPVLAWKVAMGVTIAIGVAKTVLAFVGDWARRVVPRAALLGS
                     IAGVAVLLIAFLPALKLLRDPLVGLVALTVLLLALMAGVRMPFNIPGALAAVIAGAMI
                     FWIRAWAGGPWPEHAVATRLGLALPWPTLGWLDAVDDMLPYLSVALPFAIVTVVGGID
                     NTESAAAAGDEYRARDILMTEALATVTAGLCGGVIQNTPYIGHPAYKAMGARAGYTLA
                     TGLVIGVGGAVGALSFVVAVLPEAAVAPILVFIGLEITAQAFLASPPRHGAAVALAFV
                     PTAAAVVLIQGGSLVAALGRTPADFTGEAAATWRALTVLGNGFVLTALLWAWVLVTIL
                     DRRLALTAALLAVASVATLAGVIHSPLPSGALFWPWASPSPLTAQVAGAYGVAAALCW
                     VAARRAAGR"
     gene            complement(13531..13839)
                     /locus_tag="VLF19_09005"
     CDS             complement(13531..13839)
                     /locus_tag="VLF19_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93430.1"
                     /translation="MGFSGGSYEEVARWLHNFLTSHAKREHPRLEAVLEAGGEREGHS
                     YSAWLRFGERRSGPMEFDFEDVAGHRGSLAWCKALAEQTRALAREVMAQAPRFDARAR
                     "
     gene            complement(13949..16312)
                     /locus_tag="VLF19_09010"
     CDS             complement(13949..16312)
                     /locus_tag="VLF19_09010"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HSE93431.1"
                     /translation="MHADASAGKRDHGMARPAENPAQTGRDDHARPARVGQWTDPALR
                     VLRERYLTRKDGQVTETPEEMCWRVAQSIAAGEARYGRSPAAVREIAEAFYEIMVDSY
                     FLPNSPTLMNAGKGNGLQYSACYVLPVGDSMEEIFDSVKAAAIIHKSGGGTGFAFSRL
                     RPKDDLVASTGGRASGPVSFLRVFNSATEAVKQGGTRRGANMGILKVDHPDILEFIEC
                     KLDGGITNFNISVAATNAFMDALEKGEDYELINPHTKRVVTRLSAREVFSRIVSAAWR
                     TGDPGMVFIDRINASPANPTPKIGQIEATNPCGEQPLLPNEACNLGSLNVSKFARRTD
                     GGEMTIDWDEMERVVRLAVRFLDDVIEMNPYPLPEIDRTVKDNRRIGLGIMGWADLLF
                     LLGVPYDSQEAIELADRLMAFVRETSHDQSAKLAEERGAFPNWASSIYRDVRPMRNST
                     VTTIAPTGTISMIAGCSSGIEPIFALAFEHRVKQPDGERVLTFMNETFERIARDNGWY
                     SDALKQEIAKRGAILGISGVPERAQQVFKTSHEISYDWHVRHQAAFQRYTDNGVSKTI
                     NLPNAASEDDVASAYRLAWELGCLGITVFRDGRKGEQVLNIGVGAKKDKTPVPAVGQV
                     AIKPRPHSLKGSTYRTETPIGTAYITINETYDNEAFEVFVQVGKGGSDTMAVAEAMGR
                     LVSLILRLPSPLSAQRRLEEVISQLSRIGGGQPTGFGPAKILSLPDAVARTLAEHIGQ
                     VKPSMQVPGAPTNGKKTVGDLCKECGQATFIYEEGCKKCLSCGFNEC"
     gene            16592..17920
                     /locus_tag="VLF19_09015"
     CDS             16592..17920
                     /locus_tag="VLF19_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSE93432.1"
                     /translation="MRIASAVAAGAVLVVLTLAPPAAAQQRYEPPAQRAAPPPVTGRV
                     ITMDEAVAISLDYQPQIAARLGDYAAARYRVDQALSPLLPQLTGSWAASRSQGLTRTG
                     LDTTPGIRSFADTMVARVSLSQLLFDFGKTLAATEAQRKLADVAAEDVELQRQLLALA
                     VKEAYTNILFAQRLIRVQQQALERAALNLKSARGFFDVGTRPKSDVARAEVDVANARV
                     DLIRARNAERLARVALNTAMGIDVDTPLQVQDNLVREPITIDRMAARADALDQRPEHR
                     QSRLRVAASEAAERQAFRDFFPDVTAGGSYGAARSDFSEIWEVNLALTWTFYDGGGRI
                     ARWRESKANVEASAARVKASELDIIREVEQAVYLVEEGSERIDAAQVAVNSAQENFRL
                     AQGRFDAGVGTILELTDAQLALTVAQNQETQALVDYRIALYRLDRARGRR"
BASE COUNT         2674 a         6410 c         6252 g         2761 t
ORIGIN      
        1 agcacgacgg cggggaacgc cccgtggcgg gtcacgagcg cgtcacgatg ttcacgagcc
       61 ggttcggcac cacgacgacg cggtcgatcg tgcgggcgcc gagccagggc cgcaccttgt
      121 cgtcctccag cgcctggcgc cgcacctcgg cctccgacgc gcccatgtcc acggtgagac
      181 ggctccgcac cttgccgtcg acctgcacga cgaccgtgac ctcgtccttc accagcgccg
      241 ctggatcggc ctccggccag cgctgcgcga ccacccctcc ggcccgaccg agccgctccc
      301 agagctcctc ggtgacgtgc gggcagaagg gcgccagcag gagtaccagc gtatccacgg
      361 cctcgcacat cagcgcccgg cgctcgccgg cgggtagccg gtcgagcgtc gacgcctcga
      421 aggcctggag cgcgttgacc agctccatga tggcgctcac cgccgtgttg aagtgaaagt
      481 cgcgctcgat gtcgccggtg acccgccgga tcgtctcgtg gatcgtccgc cggaaggccc
      541 tgccgtcggg cgagagcgtg gccgccgcga ccgcgggtcc ggacgcgagc tcgtccagat
      601 gggcgatcac gaagcgccac acgcggttca ggaaccggaa cgccccctcg acaccgtggt
      661 cgttccagtc gaggtccttc tccggcggcg ccgcgaagag cgagaagagc cgcgcggtgt
      721 cggcgccgta cttccggatc tgctcgtcgg ggtcgacgat gttgcccttg gacttcgaca
      781 tcttggcgcc gtccttgatg accatgccct gactcaggag cgccatgaac ggctcgtcca
      841 ctttggtgag cccgaggtcg cgcagcacct tcgtgtagaa ccgcgcgtag agaagatgga
      901 gcaccgcgtg ctcgatgccg ccgatgtact gatccacggg catccagtag tcggccgcgg
      961 ccgggtcgaa catcccgccc tcgtagtggg gcgagcagta ccgaaggaaa taccacgacg
     1021 actccacgaa cgtgtccatc gtgtcggtct cgcggcgggc gcgcccgccg cacctcggac
     1081 accgcgcgtt cacgaacgtc gcgacgtctg cgagcggcga cccgcccttg ccgctgatct
     1141 gcacgtcgcg cggcagcacg accggcaggt tttcctcggc ctccggcacc atcccgcacc
     1201 ggtcgcagta gaggatgggg atcggggcac cccagtaccg ctggcggctg atgccccagt
     1261 cgcggagacg gtagttgacg cggccctcgc cgagcccgcg ctggacgagc cagtcgatgg
     1321 ccttgcgccg agcgtcggcg acggtgaggc cgtccaggaa gcccgagttg atcttgatgc
     1381 cgtcgcccgc gtacgcctcg ccgacccagc cctcgggccg cttcacggtc tcgacgatcg
     1441 gcagacggtg ctgccgggca aagtcccagt cgcgctggtc ctcgcccgga acggccatga
     1501 tggccccggt gccgttgccc atgaccacgt agtcggcgat gtagagcggg atctcggccg
     1561 cgttgaacgg gttgacgacc ctcgcgccga ggcgcaggcc gcgcttggga cggtcggcgg
     1621 ccagccgctc gatctccgtc tggcgtgcga cttcggcgcg gaacgcggcc acccgcgcgc
     1681 gctcggcgtc gtcggccagg agccgctcga ccagcgggtg ctccggcgcc agcaccgcgt
     1741 aggtcatgcc gaacccggtg tccgggcgcg tggtgaacac gcggaggctg agcccggggc
     1801 ggccggcgac cgccaggtcg aactcggcgc cctcgctacg gccgatccag ttgcgctgca
     1861 tggtgagcac gcgctcgggc cagcctggca ggcgatcgca ccacgcgagc aactcgtcgg
     1921 cgtacgcggt gatcttgaag aaccagccgt cgatgtcgcg cggcgtcacc tcggtgtcac
     1981 agcgccagca gcggccggct tccacctgtt cgttggcgag caccgtctga cacgacgggc
     2041 accagttgac ggtcgagcga cggcggtacg cgaggccgcg ctcgaacatc cggatgaaga
     2101 tgagctgctc ccaccggtag tacgacgggt cgcacgtcgt gacctcgcgc tcccagtcgt
     2161 acgagatccc catccgcgcg agctgcgcgc gcatgttgtc gatgttctcg tacgtccaga
     2221 tcgcgggatg gacgccgtgc tcgatggcgg cgttctcggc gggcaggccg aaggcgtccc
     2281 agcccatcgg gtggatcacg ttgaagccgc gcatgcgctt gtagcgtgcg agcaggtcgc
     2341 cgatggcgta gacccgcacg tggcccatgt ggatgcggcc cgaggggtac ggaaacatct
     2401 cgaggcaata gaacttcggc cggccagggt cctcgacggc gcggaacgct cggcgctcct
     2461 gccaggcgcg ctgccacttg gcttcgatct cgtggaccgg gtaacggtcg gacgacatga
     2521 ttcggccagg atcttacggg acttagcgtg cagaggcaag aaactggccc ctcccgcagc
     2581 ccacgtgcga gaggggccgg cgcgtcagct cttcttcatc tcgagcttga tgccggcggc
     2641 cttcgccgcc tcctcgcact tcttcacgga ctcggcgtgc ttgccgtcgg cgtgcagctt
     2701 ctcggcctcg gcgatcatct tctcgacgct ggccttcttc ttcgtgtcgg acaccttggc
     2761 cacgccgtcc ttcagttgct tgatcaggag cgggcactgc cccgcctcga ccaggctggc
     2821 accgaatgcc agcgccaccg ccaccgcgat cacggccatc agcttcctca tggactgtca
     2881 cctccctccg cgccgggctg gcgctcatcg gctacaaccc gtgccgctcc cgcacggttc
     2941 ccggccgcgg ccggcgccct ctccccgcca ccgaccgccc cggagccggc gtggaagtga
     3001 tcatagatcc gttgggctag cttcggcgtg actttcggaa ccgccgcgag ctcggcgacg
     3061 gaggactccc gcacgcggcg ggcggatccc agcatcttca ggaggctcgt ccggatcgtc
     3121 ggcccgacgc ccgggattcc gtcgagggca gactggatcg tacggcgcgt ccgcagcttc
     3181 ttgtgatacg tcaccgcgaa acgatgcgcc tcgtcgcgga tcttctgcag cgtgtggagc
     3241 gccggcgacg tgggatcgag gagcagcggc tccatgctct cgccgcggta gacttcctcg
     3301 cgctgcttgg ccagcgccgc cacgggcatc cagtcgagtc cgagctcggc gagcacggtg
     3361 agccccgcgt tgagctgacc gcggccgccg tcgatgagca cgaggtcggg cagcgcctcg
     3421 ccctgctcga gcgaccgcgc gaagcgccgc cgcagcatct cggcgaggga ggcgtagtcg
     3481 tcggcgccgg cgacggtctt gatgcgaaag cgcttgtagt cgtccttctt catgtcgccg
     3541 ttctcccaca cgaccatcgc gcccacctgc tcgctgccgt ggatcgagga gatgtcgtat
     3601 ccctcgatgc ggttcggcag cccgggcagc tcgagcgcgc gctgcagctc ctcgagaatc
     3661 gcctgctggc ggttctcgcg cgacagcagg tggtgccgca gggcgatggc cgcgttggcc
     3721 tcggccatgg ccacgaactc gcgcttgctc ccccgctgcg gcacgacgag cgccaccttc
     3781 cggccggcga gcccggagag ccattccgtc gtcaggtcgg cctccggcac ctcctcggag
     3841 agcaagatct ccgacgccgg agtcacctgc ttgccgtaga actgacgcac gaacgccgac
     3901 aacacctcgc cgtccatcca cccggagacg cgatcgaaga agaacgcttc ctgccccacc
     3961 aggcgcccct tgcggacgaa gaacagctcg acgcacgcgt cggccccctg gcgcaccacg
     4021 ccgaggacgt cctggtccac gagatcggtc gagatgatct tctggcgctc ccgcaccttg
     4081 ttgagcgact ggatctggtc gcgcagcacc cccgcccgct cgaacttctc ctcgccggcc
     4141 gcccgctcca tctcacgggt gagccggacg gcgagatcgt cgtctcgccc gtccaggaaa
     4201 tcctgcacct cgcgcaccgt gcgcgcgtag ccctcgtgcg tctcccaccc cgtgcagggc
     4261 gcgttgcagc ggtggatgaa gtactggatg cacggtcgct cgagtcggcc gtcgatcttg
     4321 atcgcgcagg tccgcagcgg gaagagctga cgcacgagcc ggagggtgtc acgcatggcc
     4381 gtggccgggt agaacggccc gtagtactgc gcgccgtcgc gctggacctt ccgggcgacc
     4441 agcagccgcg gatactcctc gttcgtcgtg agccggagga acggatagtg cttgtcgtcg
     4501 cggaggatga tgttgtagcg cgggcggtgc ttgcgcacga ggttgacctc gagcatcagg
     4561 gcctcgagct cgttgtcggt caccacgtac tcgaggtcgg ctacctggcg caccaacgcg
     4621 tcggtcttgg cgtcgcgggg ccgagattcc tggaagtacg agcgcacgcg gcctcgtagc
     4681 gacgccgcct tgccgacgta cagcacctgt cctctggcgt ccttgtagag gtagacgccc
     4741 ggccgctcgg gcagcgtctg gagcttctcc tccagcgtca tgcgcccatt gtagcggtga
     4801 cccgccgtcc gaggctcgcg cgccactccg gcgggctcgg gcgacgcgag ccggccccac
     4861 cgcgtgcgga cgacgtgggc cccgccgaac gcgtatgggg accgatgctc gacaacacgc
     4921 agcgtcgccc cggagagccg gacgcgctgg acgacccggc gacggcagcg aggcgcgggt
     4981 cgggggaagg tcctccgaaa gcctcctccg aggatctaca cggtctgtag cttccggagc
     5041 gcgcgcacgc ggtcgcgcag ctccgcggcc tgctcgaagt cgagccgccg cgccgcctcc
     5101 ttcatcttcc cgtcgagctc cgcgatgcgc cgctcgaggt cggccggcga ctcgaaggtc
     5161 tcgggcccgg gctccgccac ctcgaccgtg tagtagtcgc gctcgtagat cgtctgcagc
     5221 agctcccgga tcgacgtccg gatcgactcc ggggtgatgc cgtgctcggc gttgtagccg
     5281 gcctggagcg tgcgccggcg ctcggtctcc cggatcgtgg cggccatcga ctgcgtcacg
     5341 tggtcggcgt acatgatgac cttgccgttg acgttgcgcg cggcgcgccc ggccgtctgg
     5401 atcagggagg tctccgagcg gaggtagccc tccttgtccg cgtcgaggat cgccaccagc
     5461 gccacctccg ggatgtcgag gccctcgcgc agcaggttga tgccgatgag gcagtcgaac
     5521 ttgcccagcc gcaggtcgcg gatcactgcc acgcgatcga gggtgtcgat gtcggagtgc
     5581 agatagcgca cgcgcagccc cgcctgctgg tagtactccg tgaggtcctc ggccatgcgc
     5641 ttggtgagcg tcgtcaccag cacgcgctcg gcgcgctcgg cgcgcacgcg gacctcgtgc
     5701 agcaggtcgt ccacctgctc ggtcgccggc cggaccatga tctccgggtc catcaggccc
     5761 gtcgggcgga tcacctgctc ggcgagcgcg tccccggcga gctccagctc gtaggggccc
     5821 ggcgtcgccg acacgtacac ggtctggccg gtgagacgcg agaactcctc gaaggtgagc
     5881 gggcggttgt cgaacgccga gggcaagcgg aagccgtact cgaccagggc ctccttgcgc
     5941 gagcgatcgc cgtagtacat gccccggatc tggggcaccc ccacgtggct ctcgtcgatg
     6001 atcaccaggg catccttcgg caggtagtcg atgaggaccg ggggcggctc gcccgggcgc
     6061 cggccggtga ggtgccgcga gtagttctcg atcccgtggc agaacccgag ctcgcgcagc
     6121 atctccatgt cgaagagcgt gcgctgctcg aggcgctgcg cctccagcaa gcggttccgc
     6181 tgccggagga aggccaggcg ctcgcccagc tcctcgtgga tggtccccag cgcgcgctcg
     6241 agctgcgcgg ccggcgtgac atagtggctg gccgggtaga cgtgcacgtc ggggaggcgc
     6301 tcctgcactt ggcccctgag cggatcgatg cggtggatcg cgtccaccac gtcgccgaag
     6361 agctcgatgc gcagcgcgtg cgtctcctcg ttggcgggaa agacctccac gatgtcgccc
     6421 cgcacgcgga aggtgccccg gtggaagtcg taatcgttgc gctcgtactg cacggcgatg
     6481 agccgccgga tgatctcgtc gcggtcgatc tgctcgccct cccggagcgc caggtgcatc
     6541 tcctggtacg tctcgggcgc gccgatgccg tagatgcagg acaccgaggc cacgaccacg
     6601 acgtcgcgcc gctccatcaa cgagcgcgtg gccgagtggc gcatgcggtc gatctcgtcg
     6661 ttgacgagcg cgtccttctc gatgtacgtg tccgactggg gaatgtaagc ctcgggctgg
     6721 tagtagtcgt aatacgagac gaagtactcg acggcgttct ccgggaagaa ggccttgaac
     6781 tcgctgaaga gctgggccgc gagggtcttg ttcggcgaca gcaccagcgt gggccggccg
     6841 gcgtttgcga tgacgttggc catcgtgtag gtcttgccgg atccggtcac gccgcgcagg
     6901 acgcagtggc ggcgcccgcc gcggatcagc tcggtgagcc tggcgatggc ctggggctgg
     6961 tcgccccgtg gggggaactc cgacgtcagc ctgaacacga ctctgatcgg gcggccgcgt
     7021 cgtcgcgacg ggtcaatgcg gcggagcgcg ggccgtggtg ggaagcgagc agcgccgccc
     7081 tgcgcggcca gcggtgaggt ggccggctgt cccgtccggg tgcccgtacc cggtctaggt
     7141 cgacgtgggg accgcgagca ggtacttgcg ccacgagtcg agcagcgagt gcgcgtgcgg
     7201 gtgcttgagc atgtgcgtgg agaacacgaa cttggggtgc acgggctcgc gcagcagacg
     7261 catcccgacc tcgtccagca ggcggttgct cttcttgagg ttgcagcgca ggcacgcggc
     7321 cacgacgttg gtccagctcg tctccccgcc acgggagcgt ggcatgacgt ggtcgacggt
     7381 gaggcgctcg ccgcggcggt tgcagtactg gcaggtgtag ccgtcccgcc gcaggatgtt
     7441 cttcttgttg aaggccaccc tgtcgaggaa cggcttgcgt atgtacaccg ccaggcggat
     7501 caccgccggc agcgcgaagg cgcgtgacgg cgagcggacc acccggggcg agccctccac
     7561 ggcttcggcc ttgccggcga tgagcagcgt gatggcgcgg cgggcgttgg tgaaatgcag
     7621 cggctcgtag gtgtagttca gcaccaggac cgcgatttca tccatcttgt cagaaaagga
     7681 tagtcggcgg cccatgacgt gtcaaatctg ccggggctac cgccgcacga ggttcctcag
     7741 cacgaatgcc acgtttgccg gccgctcggc gagccggcgc atgaagtagc cgtaccagtc
     7801 ctccccgaac ggcaccagca ctcgcacggc gagcccctgg gcggagaggg ttgcgtgcag
     7861 ctcactccgg atcccgtaga gcatctggat ctcgaagcgc tcccgccgca cccggagcgc
     7921 cccggcgcgc tcgcgcacgt gcgcgatgcg ccgctcgtcg tgcgtggcga agcccgggta
     7981 gacgctccgg gcgagggcgt ctggggcgag gagccgatcg accagcgcgt cgtaggcgcg
     8041 gtcgacgtcg gccttgtccg gatgggcgat cctgggcggc tcgcggtagg cccccttgca
     8101 gaggcgcacc gccgcgccga ggtcgatgag ccgctcgacg tccacaggtg tccggcgcag
     8161 gtaggcctgc accacgcagg cggcgtgcgg gtgggcgggc cggagacgtg cgtagaggtc
     8221 cagcgtgcga tcggtgtacg cggccgactc catgtcgatc cacacgcgcg tgcctaccgc
     8281 gcgcccgcgc tcgagcacgc gcgtgaggtt ggccacgcac accggctcgc cgaggtccag
     8341 gccgaggtgc gtgagcttga gcgagggcaa cgccgtcagg ttccggcgct tgatctcgtc
     8401 cagcagcgcg aggtacgtgt ccgtcgcgtg caccgcctcg gccgccgtgc ggacgttctc
     8461 cccgaggtag gtgacggcgg ccaggagacc gcgcgcgttc aggtcggcga cgaccgccag
     8521 cgcctcgtcg gcggtcgtgc cggccacgaa gcggcggacg aaccggcgag tgaggggcag
     8581 ccggtccatc cactgcccga ggcggggccg cgtggacagc gcgaggagca gcgcgcgcga
     8641 gatcatcgcc atcgctcgac cgcctcggcc atgatacctt cgcgcagccc ccagtcgctc
     8701 accaccacac cggccgtgcc cgtcgcgtcc agcgccgcca gcacgatcgc cacgccgggc
     8761 acgatgaggt cggcgcgccc gggctcgaga cacggcaggt ctccacgctc ccgcaccgtc
     8821 agggcgctca aacggtcgcg cagccgctcc accgcggggc gcgccagccg gtggccctgc
     8881 acgcgagcag cgtcgtaggc cgcgagcccg aggtccagcg cggccagcgt ggtcacggtg
     8941 cccgcggtcc ccacgagatc ggccaggccg gaacaacgga tcgtgacggg gagctcggtg
     9001 gcgagccgct gccggatctc gcgctcgagc gcgccatagc ggcccgcgtc gacggctccg
     9061 gggaacggat accgctccgc gagcggaacg accccgagcc cgaggctgac ggtggccgtg
     9121 acctggccgt cgcgagcgag ggtgaactcg gtgctgccgc cgccgatgtc gaaggccagc
     9181 atcgagccgg caccacgacc gagcccggcc gcgatgccgc gcaccgtgag gcgcgcctcg
     9241 tcctcgccgc tcaccacgtc gacgcggacg cccgccgcgc gcgcgagcgc cgtgacgaag
     9301 tcgccaccgt tgacggcctc gcgcacggcg ctggtggcga cgacccggac ctcccgggcg
     9361 ccggcccgca ccgcgcgtcc ggcgtactcg acgatcgtgg ccgccgtgcg tgccatcggc
     9421 gtctcgccca gccgcccgct cgccgcgagc ccctcgccga gacgtgtcac gcgctggtcc
     9481 tggtccacga cccgccagcc gccggcgtca ccgacttcga cgacgagcag gcggacggtg
     9541 ttgctgccga ggtcgatgcc ggccagccgc ggtgacgtca ccgcggccgg ccggcgcgtg
     9601 gacggattgc ggctacttct tgagcgtgcg gatgtagttc acgatctccc agcggtcctt
     9661 ctccggcagg tgcttccacg gcggcatcgc gccgcggccg ttggagatct tccagaacag
     9721 ctcgccgtcg gtctctttct ggaccctggc cgaggtccag tccgcaggct tgggaggcgg
     9781 cagcgcggcg gcggccacgc cgtcgccctt gcccgaggcg ccgtggcagg tgacgcagtt
     9841 ggtctcgatg ttcttcttgg cgttccccac gcccttcacg gggttcttca tgttcttggc
     9901 gtcggcgggg gccttccatt ccccctgtgc cgagaccgcc gcgaccccgg cccccgcgac
     9961 gagcaccacc gccgccaggg cgatggcccc acacctcccg agcctgctca tggtcttcct
    10021 ccatggacga ccgtcatgaa gttcgcacac gacgccgcac attgtcacca gtgggacggc
    10081 gagaatcaag gcgctattca cgtcccagcg ccgcgtggat ttcggccagc cgcgtgaact
    10141 tcacgcgcgg ccggcccatg acgcggccgc gcgcggtctc gagcgcgtcc agccgcctcc
    10201 agtcgacata cgatacgacc cgcgcctggc gcgagcgcac cagccggtcg gccgccgccg
    10261 cgccgggctc ggcgggatcg agcacgtggc cggcggcgag atcctcgagc atggcggccc
    10321 cggtctccgc cgcatcgggc ttgttcgtcc cgatcacacc ggtcggcccg cgcttgatcc
    10381 acccggccgt gtactcgccg aggcgcggcc gccgcgtgtc ggggtcgagc acgcgcccct
    10441 tctcgttgag gatcacgccc cacgactcgt tgaagggcac gccgggcagc ggcacgccac
    10501 gatatccgac ggagcggaac acgaggcccg ccggcagcgt ctcgacctgt ccggtcggct
    10561 tggcctgcag cgagccggtc tccgtggcca cgagttgatt ccggacgagc gtgaggccgg
    10621 tcacctgccc cgcgtcgttg ccgagcacct cgatgggcga cacgaggaag cgcagcgcca
    10681 ggcgcctggg cttccccgtc ggcggtcgcc gtgcgtactc ctggaggatc tcgaccttct
    10741 tgcgctcggc gcggtcgccg ctcttctcga gcgccgcgcg gctcagctcg tccagctcga
    10801 cctcgtcggg ccggacgacg acgtcggccg ccgcgagctc gcccagctcc ctgagctcgg
    10861 ggttggtgaa ggccgcctgc gcggggcccc gccgccccag caagacgacc tcgcggatcc
    10921 ggctcgcgcg gagcgcctcc agcgcgtggt cggcgatatc ggtcctctcc agctcctccg
    10981 ccgtgcgcga caggatgcgc gcgacgtcga tggcgacgtt gccgacgccc acgatcgccg
    11041 cccgctcctg cgaaaggtcg aacgtgagat cgcggtagtc gggatggccg ttgtaccagg
    11101 cgacgaactc ggtcgccgga tggctccgcc gcaggtcctc cccggggatg cccatccggc
    11161 ggtccgtctg cgcgcccgtg gaatagagga tctggtgata gtgacggcgg aggtcctcga
    11221 cggagacgtc cttgccgagc tcgacgccgc cgtaaaagcg gaagcgcggg tgcgcggcga
    11281 ccttgtcgaa gaccgcggtg accgccttga tcttctggtg atccggcgca acgccggctc
    11341 gcacgaggcc gtagggcgtc ggcagccggt cgaacacgtc gacctcgacg acggcgccgt
    11401 cctggcgtag caggtggtcc gccgcgtagt acccggcggg gccggccccg acgatggcca
    11461 cgcggagcgg acgctccgtc gtccccggac gctcggtcac gcgcggtctt tcaagagctg
    11521 gacctgcagc gtgtccacct cgccctggta gcggtcgcgc tgcgccttga cgatgtcccc
    11581 gatggacacg atgccgacga gcttgccgcc gtccatcacc ggcaggtggc ggatccgccg
    11641 ctgggtcatc gtggagccca cggtggcgag gtcgtcggcc ggagcgccga cgacgacgtc
    11701 gcgggtcatg atccggctca ccggccagtc gaagacccgc tcgtcgcgcg ccagcgtcct
    11761 cacgacatcg cgctccgaga cgatgccggc gggccgcccc tcgccggtcg cgacgaccac
    11821 cgcgccgatg ttgcgctccg cgaggagtgc gagcgcctca cggatcgtct gctcggggcg
    11881 gaccgtcacg acctccacgc ctttgctggc gagcacactc gcgatgttca tggcacacct
    11941 ccgggagggg atctgggccc ccatcttagc cgccgcgggg cggcgccgaa aggctcaccg
    12001 cccggcagcg cgtcttgccg ccacccagca cagcgcggcg gcgacaccgt aggcgccggc
    12061 gacctgcgcg gtcaacggcg acggcgaggc ccaaggccag aagagcgcgc cactggggag
    12121 cggcgagtga atgacgccgg cgagcgtcgc cacgctcgcg acggcgagca gcgccgccgt
    12181 gagggcgagc cgccggtcga ggatggtcac gagcacccag gcccacaaga gcgcggtcag
    12241 cacgaagccg tttccgagga ccgtgagcgc ccgccacgtg gcagcggctt cgcccgtgaa
    12301 gtcggccggc gtccgcccga gcgcggccac gaggctgccg ccctggatga gcacgacggc
    12361 ggcggcggtg gggacgaagg ccagcgccac cgcggcgccg tgacgcggcg gcgaggccag
    12421 gaacgcctgg gcggtgatct cgaggccgat gaagacgagg atcggcgcga cggcggcctc
    12481 cggcagcacg gcgaccacga atgacagcgc gcccaccgcc ccgcccacgc cgatgacgag
    12541 cccggtggcc agcgtgtagc ccgcgcgcgc gcccatcgcc ttgtaggccg gatggccgat
    12601 gtacggagtg ttctggatga ccccgccgca gaggcccgcg gtgaccgtcg ccagggcctc
    12661 ggtcatcagg atgtcccgcg cgcggtactc gtccccggcg gccgccgccg actcggtgtt
    12721 gtcgatgccg ccgacgacgg tgacgatcgc gaaggggagc gcgacggaga ggtacggcag
    12781 catgtcgtcg accgcgtcga gccagccgag cgtcggccac ggcagggcga gccccagacg
    12841 cgtggcgacc gcgtgctccg gccacggccc acccgcccag gctcgaatcc agaagatcat
    12901 ggcgcccgcg atgactgctg ccagcgcacc cgggatgttg aatggcatcc ggacgccggc
    12961 catgagcgcg agcagcagca cggtcagggc gaccaggccg acgagcggat cgcggaggag
    13021 cttgagggcg ggcaggaagg cgatcagcag caccgcgacc ccggcgatcg atcccaggag
    13081 cgcggcgcgg ggcaccacgc ggcgcgccca gtcgccgacg aatgccagca cggtcttggc
    13141 cacgccgatc gcgatcgtca cgcccattgc caccttccat gccagcacgg gatccccggt
    13201 cgccatcttc acggggccca gcacgccgaa gaccatggcg aagagcgtgg gcgtgtcgat
    13261 gccgaacggc atcgcggtga cgtccgcgcg gccagtgtgg cgcatgaggc gcaccgccaa
    13321 ccacgtgtag gcgaggtcgc cgaccagcac gccgagcgcc gttcccggga ccatccggta
    13381 caggacgagg tctggcggga aggcgaagac gccgatcagg aggctcgaga gaatgacgag
    13441 gttggtggtg ttgtcgaggg cgagaccgag aaacccgttg aggtcgccgg agcggatcca
    13501 gcgcgggcgg actcccgcac gcgccgccgt ttagcgggcc ctcgcgtcga accgcggggc
    13561 ctgcgccatc acctcacgcg ccagcgcgcg tgtctgctcg gccagcgcct tgcaccacgc
    13621 gaggctgccc cgatggccag ccacgtcctc gaagtcgaac tccatcggcc ccgaccggcg
    13681 ctcgccgaac ctgagccacg cactgtacga gtgcccttcc cgctcgccgc ccgcctcgag
    13741 aacggcctcg aggcgcgggt gctccctctt ggcgtgagag gtgaggaagt tgtgaagcca
    13801 ccgcgcgacc tcttcgtagg acccgccgga gaaccccatg gcgagaggct ataccccgtg
    13861 gggtcttatc ggtgggattc cgggaggctc gtcgccagcc ctccggcaag atcggtcgtg
    13921 tcgagccggc catcgatcga tcgaccagct agcattcgtt gaatccacaa ctcagacact
    13981 tcttgcagcc ctcctcgtag atgaaggtcg cctggccgca ctccttgcaa aggtcgccca
    14041 cggtcttctt gccattcgtc ggcgcgccgg gcacctgcat cgacggcttc acctgcccga
    14101 tgtgctcggc cagcgttcgg gcgaccgcgt cgggcagcga caggatcttg gccggcccga
    14161 agcccgtggg ctggccaccg ccgatccgcg agagctggct gatgacttcc tccagccgcc
    14221 gctgagccga cagtggcgaa ggcagcctca gaatgagcga aacgagacgc cccatggcct
    14281 cggccacggc catggtgtcc gagccgccct tgccaacctg gacgaacacc tcgaacgcct
    14341 cgttgtcgta ggtctcgttg atggtgatgt atgcggtgcc gatcggcgtt tccgtccggt
    14401 aggtcgagcc cttgaggctg tgcgggcgcg gcttgatcgc cacctgcccg accgccggga
    14461 ccggcgtctt gtccttcttg gcgccgacgc cgatgttgag cacctgctcg cctttgcggc
    14521 cgtcgcggaa cacggtgatg cccaggcagc ccagctccca cgccaggcgg taggcgctgg
    14581 cgacgtcgtc ctcgctcgcc gcgttcggca ggttgatcgt cttgctgacg ccattgtcgg
    14641 tgtagcgctg gaacgcggcc tggtgccgca cgtgccagtc gtagctgatc tcgtgggagg
    14701 tcttgaacac ctgctgcgcc cgctccggca ctcccgagat gccgaggatg gcgccccgct
    14761 tggcgatctc ctgcttgagg gcgtcggaat accacccgtt gtcacgcgcg atgcgctcaa
    14821 acgtctcgtt catgaacgtg agcacgcgct cgccgtccgg ctgcttgacc cggtgctcga
    14881 aggccagcgc gaagatcggc tcgatgccgg aggagcagcc ggcgatcatc gagatcgtcc
    14941 ccgtcggggc gatcgtggtc accgtggagt ttcgcatcgg ccggacgtca cggtagatcg
    15001 agctggccca gttcgggaac gccccgcgct cctcggcgag cttggccgac tggtcgtgcg
    15061 acgtctctct cacgaaggcc atgagccggt cggccagctc gatggcctcc tggctgtcgt
    15121 acggcacgcc gagcaggaac aagaggtccg cccagcccat gatgccgagt ccgatgcgcc
    15181 ggttgtcctt caccgtcctg tcgatctcgg gcagcgggta ggggttcatc tcgatgacgt
    15241 cgtcgaggaa gcggacggcc agccgcacca cccgctccat ctcgtcccag tcgatcgtca
    15301 tctcgccgcc gtcggtccgc cgcgcgaact tcgagacgtt gagcgacccg aggttgcacg
    15361 cctcgttggg cagcagcggc tgctctccgc aggggttggt cgcttcgatc tggccgatct
    15421 tcggcgtcgg gttggcgggg ctggcgttga tacggtcgat gaagaccatg cccgggtcgc
    15481 ccgtgcgcca cgcggcggag acgattcgac tgaacacctc gcgggcggac agccgggtga
    15541 ccacgcgctt ggtgtgtggg ttgatcagct cgtagtcttc gcccttctcg agcgcgtcca
    15601 tgaacgcatt cgtggcggcg accgaaatgt tgaagttcgt gatgccgccg tcgagcttgc
    15661 actcgatgaa ttccaggatg tcgggatgat cgaccttcag aatgcccatg ttcgcaccgc
    15721 gacgggttcc gccctgcttc acggcctccg tcgcactgtt gaacacacgc aggaacgaga
    15781 cgggaccaga tgcgcgacca ccagtcgagg cgacgagatc gtccttggga cgcaggcggg
    15841 agaacgcaaa acccgttccc ccgccactct tgtggatgat cgccgcagcc ttcaccgagt
    15901 cgaagatctc ctccatcgag tcgccgaccg gcaggacgta gcaggcggag tactgaagac
    15961 cattgccctt tccggcattc atgagcgtgg gggagttcgg cagaaagtag ctgtcgacca
    16021 tgatctcgta gaacgcctcg gcgatttccc gcaccgccgc cgggctccgt ccgtaccggg
    16081 cctcgccagc cgcgatggac tgtgccaccc gccagcacat ctcctccggg gtctcggtga
    16141 cctgaccatc cttcctcgtc aggtagcgct ctcggagcac acgaagggcg gggtcggtcc
    16201 actggccgac tcttgccggg cgagcgtgat catccctgcc tgtctgagcg ggattctcgg
    16261 ccgggcgggc catgccgtgg tcacgcttcc cagcgctcgc gtcagcgtgc atcgtctgtc
    16321 ctccttcgag cgccgcctga acccgaccaa gtccctgctt cagggctgtg ggggccgtgg
    16381 ctccaagctc atgttacccc aatatcttgt ggtgaaccgc aatctatccc caggatttcg
    16441 cgcccgtcaa gaaaaaaggt gtccaccgtg gatccaccgt gcatccaccg cgcctggccc
    16501 agaaaaagcg ccggccacgc cgcctaccga acccgcgtga tattcttcac accgtaaagt
    16561 agtcgtgaca gcgctggcaa ggagcccttc gatgaggatc gcgtcggcgg tggcggcggg
    16621 cgccgtgctg gtcgtcctga cgctcgcgcc gccggcggcg gcccagcagc gttacgagcc
    16681 ccccgcgcag cgcgccgcac cgccgccggt caccggccgc gtgatcacga tggacgaggc
    16741 cgtggcgatc tcgctcgact accagccgca gatcgccgcg cgcctgggtg actacgccgc
    16801 cgcgcgctac cgcgtggacc aggcgctgtc gccgctgctg ccgcagctca cgggctcatg
    16861 ggccgcctcg cgctcgcagg gcctcacgcg cacggggctc gacacgaccc cgggcatccg
    16921 gtcgttcgct gacaccatgg tggcccgcgt ctccctgtcc cagctcctct tcgacttcgg
    16981 caagaccctc gccgccacgg aggcccagcg caagctcgcc gacgtcgccg ccgaggacgt
    17041 agagctacag cgccagctcc tagccctcgc ggtgaaggag gcctacacca acatcctgtt
    17101 cgcccagcgg ctcatccggg tgcagcagca ggcgctcgag cgcgccgcgc tgaacctcaa
    17161 gtcggcgcgc gggttcttcg acgtgggcac gcgccccaag tccgacgtcg cgcgtgccga
    17221 ggtcgacgtg gccaacgcgc gggtcgacct catccgcgcc cgcaacgccg agcggctggc
    17281 gcgcgtggcg ctgaacacgg cgatgggaat cgacgtggac accccgctcc aggtgcagga
    17341 caacctggtg cgggagccga tcacgatcga ccgcatggcc gcccgcgccg atgccctcga
    17401 ccagcggccc gaacaccggc agtcccgcct gcgcgtggcc gcctccgagg ccgccgagcg
    17461 acaggcgttc cgcgacttct tccccgacgt caccgccggc ggcagctacg gcgccgcccg
    17521 ctcggacttc agcgagatct gggaggtgaa cctcgcgctg acgtggacgt tctacgacgg
    17581 cgggggtcgc atcgcgcgct ggcgcgagag caaggcgaac gtcgaggcct ccgcggcccg
    17641 cgtgaaggcc agcgagctcg acatcatccg ggaggtcgag caggccgtct acctagtgga
    17701 agaagggagc gagcgcatcg acgccgcgca ggtcgccgtg aactcggcac aggagaactt
    17761 ccgactggcg cagggccggt tcgacgcggg agtgggcacg atcctcgagc tgacggacgc
    17821 tcagctggcc ctcaccgtcg cccagaacca ggagacgcag gcgctggtgg actatcgaat
    17881 cgcgctctat cgtctcgacc gggcgcgcgg ccggcggtag tctggagcag gttcaggccc
    17941 gcccgggctg aacggaaaat acgcacggcc cgcgggccgt ccaagatgtg atgagacgga
    18001 cgcttttcct cggtgcggtc atcctcggcg tcgccgccgc cgtgtggggc tacctctacg
    18061 tccagagccg gggcaacgcg ccgcagtacc gggtcgg
//
LOCUS       DATIAZ010000251        11073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1251551, whole genome shotgun sequence.
ACCESSION   DATIAZ010000251 DATIAZ010000000
VERSION     DATIAZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251551"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VLF19_09020"
     CDS             <1..131
                     /locus_tag="VLF19_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSE93433.1"
                     /translation="FVLYPGAPHAFFSDDRPQVYKKEAAEDGWKRCLAFFDKHLKA"
     gene            complement(133..993)
                     /locus_tag="VLF19_09025"
     CDS             complement(133..993)
                     /locus_tag="VLF19_09025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSE93434.1"
                     /translation="MATSDYRMSDADVLLRRALEGRGDERILTLDPAFSGLPDTAHGG
                     SVLAVFDALAARVGGREVRGVYRRRVPLGVPLRLTVVGDGDALECRLAETSETVLVEG
                     RIAPVAAPVGTGRPPAALPADPLPVSTTCFACGVDNPIGLRLRLSFDDRAVAGRWQPG
                     PAFRAANGALAPVALTTMLDEAAFWLGALASGESGMTTDLSVTLHDPVPFGDVVTVGG
                     CRDAVRPLADDPRYWATEVTAWDAAGRLVADARITFVAVRGAARRLAGWLSRVNPPDV
                     VRRVFPAYAR"
     gene            986..2182
                     /locus_tag="VLF19_09030"
     CDS             986..2182
                     /locus_tag="VLF19_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE93435.1"
                     /translation="MAILAHRAFVRATVTNFFFFASLNGFVLLPLYILRLGGTEVEIG
                     LVMALHSGVGIVCQPLLGPWVDAAGRRPFMLLGVGLVLLSALLATAATSIAWLAVVRA
                     LQGIGFSAFFVANYTYVIDLVPAERRGWALGIYGVSGMISTALTPLVGEAIIRTLGFR
                     PFFGLCALLAAVSGVLVWQLAEPARAEAPTTRAWEIARAGVGDMLQLHMAVTVFFGLG
                     TGTVFVFLPTFAEDLGVTTLALFYTAYAMAAMGVRVVAGGLIDTRGRRAVIVPSMFLQ
                     AAAAALLAGLGAAVTRTSPAPALPVLIVAGLMSGAAHGFLYPGLAALVADRAPPERRG
                     AVVGVFSAVFLVGNAGGAFAFGYVAHAFGYAAMWGLLAALLLAGASLSLRLAEDTRLR
                     PLRAAE"
     gene            2483..3025
                     /locus_tag="VLF19_09035"
     CDS             2483..3025
                     /locus_tag="VLF19_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSE93436.1"
                     /translation="MSTTCRFGGVALLALALTCVATPGDGARDAASPPAPRPGGPQEA
                     EVVGVAVDPRTQVPALVLQGKRDKRNVVMSIDPAQMLAISLPLQGVTPPRPLTHDLFL
                     SLFARLKVTLTRVVITDFRDDVYYALLHLSSDGTDLTLDARPSDAVALAIRAKVPVLV
                     EDRVFDRGGIDKPAPGRPSI"
     gene            3029..3967
                     /locus_tag="VLF19_09040"
     CDS             3029..3967
                     /locus_tag="VLF19_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSE93437.1"
                     /translation="MAPDTDGLEWALQRRLEDVFSKLGEKFGDLTGRIETTIRPDVSF
                     DDVGGLGAAKEVVRGFTHALVEPDRYRNWGIAPPKGVLLYGPPGTGKAMLARALAGAS
                     EAIFFRLLLHNLTAKFGANTAELLQEALRIAVGEGRAVFLLDQVDALSLDHLLPPPQA
                     REASARLVAALCEKLDALDPASRVLVVAATSRTDAIDPALVAPGRLDHLVEIPLPDAA
                     AQQEILELIQARMEKSAGRPLFAPLDYRTVLPLMGGMSGADISDILGRALEAKVHREA
                     AEGTASPVGTADLLEAIDAYKRVRGVVEKIRYGQYL"
     gene            3969..4400
                     /locus_tag="VLF19_09045"
     CDS             3969..4400
                     /locus_tag="VLF19_09045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSE93438.1"
                     /translation="MERPQTVTTTLRVRYKDTDCMKVVYYGNYLTYFEVGRVEYLRQR
                     GMPISEVDRRVHMPVVEAHVRYVRPARLDDLLEIRCWVSERRRASFRFAYDVRSEAGD
                     TIASGWTLHACWDPATSRMIALPPWLSALMPVAPDTPAERV"
     gene            4426..5397
                     /locus_tag="VLF19_09050"
     CDS             4426..5397
                     /locus_tag="VLF19_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93439.1"
                     /translation="MIEVQNLTKHYGPVTAIRDVSFAVAPGAIVGFLGPNGAGKSTTM
                     RILSCFMPASSGTARVAGYDVFKESMEVRRRIGYLPESVPLYADMRVAPYLDFVAEVK
                     GLGRADRRRRVGEVMERCLIADVQNRLVGKLSKGYRQRVGLAQAIISDPHVLILDEPT
                     IGLDPRQITEIRSLIKSLAGEHTVILSTHILPEVSMVCSGVIIINKGSIVAQGPIDTL
                     VEQFFPTARVQVEIRGPAAAVREGMQRLPGVLEVREQEVMDGHGVYIVESSRDRDVRA
                     EIFQLAAQQKWELRELRRVGMTLEEVFIRIVAGEETDERAAPAEAAS"
     gene            5394..6161
                     /locus_tag="VLF19_09055"
     CDS             5394..6161
                     /locus_tag="VLF19_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSE93440.1"
                     /translation="MKVWPIFKKEMRLYFTSPIAWVILTIFLLISGYFFYNIFAFFTM
                     ASMQSAMNPAMARELNVTDSVMRPLFSNISVILLLLMPLVTMRLFAEERRAGTIELLL
                     TYPVRDGAVLIGKYLAALALYAVMLVLTLVYPAMVVYFARLEWGVLLTGYLGLLLMGA
                     TFLAVGIFASSLTENQIVAAIVTFGVLLIFWVIGWSADYAGGPWSRVLSHVSLLEHFD
                     SFAKGVLDTKDIIYYLNFTLLALFLTLRSLEARRWKG"
     gene            6169..7719
                     /locus_tag="VLF19_09060"
     CDS             6169..7719
                     /locus_tag="VLF19_09060"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HSE93441.1"
                     /translation="MRRLFDWAGYVGLAALAALAILNWTRPPFMTGTRATWIWWSLLV
                     GGVLLVLISLLAYARGSGVFGGRTARYGLNTALAVVLLLAIVGLVEAVSYRHNARLDL
                     TENKRHSLSPQTIQLLRDLKTDVNAVAFFRSDQPNKRAAEDLLKQYQRYAGSKLTYRV
                     VDPDREPGLARRYAVESYGTVVLETKDRSEKVLDAEEEKLTNGLVKLTREGKRVVYVV
                     QGHGERDLASTDRPGFGEAKTAMERANYEVKPLALARQDKIPEDASVVIVAGSRTELL
                     PPELGALEAYVGRNGKLFVMVDPGIIGGVQAEGLLRFLGKYGFELGNNLVIELNPIGR
                     LFGIGPEVPIIQQYETHAITRDMAGISTLFPLTRTVEVAKAPPAGIVLTPLARTSTES
                     WAETDRASLQAGQVKADPQDLKGPVTVAAVATKDKARIVVYGTSSLAANQFLNLQGNR
                     DFFLNTVSWLAEQEDLIAIRPKDARQTPVFMTSRQAQLAFLVPVVVLPGVVLAGGIFT
                     LVRRRAAR"
     gene            7849..9615
                     /locus_tag="VLF19_09065"
     CDS             7849..9615
                     /locus_tag="VLF19_09065"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSE93442.1"
                     /translation="MRWQTTAFLAILLIAVGTFYYMYEVRWAPDREQAQSRKGRVFDV
                     DAADVTELTLKRGDEVVRLVRESDGWQMTAPLAWRGERGPIEETLTSVATARMDREIA
                     AVPADVAEFGLDKPAAEVTLATKGGKQLGLRLGAKSPTGVWVYAQERGKPAVFVVGDG
                     VLRDATRPLADFRSKAVLSFDRKDVTGVEIVGRDETLAVEPAGERGWRMTRPRALEAD
                     PDAVVELLEKLAGARIREFVAETPRSLAPYGLERPVRVAIHVGKDKDRATRTILLGNT
                     DDKKKGVYALRPGETTVLLLPEDVSKAVPRTTAALRNKTVIPIERDKVGGLEVESPRG
                     AVTLVREQDKWRITQPEALPADQVEAGAVLMRLVNLKALAFLSEDASAIPRYLAKPEV
                     RVTIGQQGEASSQTLLLAPSPEKRGGQATAYAAVAGRGPVVLVDASALTEIGRPLRQL
                     RDRTLVAGLEPRDVKRVHVKRDGKAVLVERTGDTEWRVLEGGKGTANTAKVEDLLYGL
                     RNLKWQDIAAPDGGEAAKFGLAAPAAEVTLYRADGTAIATLLVGGQEGDRRYVQMKAA
                     PTVYAIDAKQLTLPKVPEDFQS"
     gene            complement(9796..10758)
                     /locus_tag="VLF19_09070"
     CDS             complement(9796..10758)
                     /locus_tag="VLF19_09070"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSE93443.1"
                     /translation="MTTAQQAARRVTYQLETLWDYDYVPTHEELATLYETAKKNQWNG
                     STAIDWSRPVGTEGPVLNVQLAFGGTNFFSRLTPEEQKEVEIRVSAWRLSQFLHGEQG
                     ALVVCGQLVNSIPELDAKLYAATQVVDEGRHVEVFEKYVKKLHKIYPVDPLLKALIDE
                     ILATNLWELKLVGMQMMIEGLAIAAFNVMRKQTADPTLSQLLDYVLQDEGRHVNFGYF
                     ALRRSIPDLDPVKRAYLEDFCLKACDLMYARDERTGFKSIQNVWNEMGWDGEKIWRET
                     VATSPTTKVFNSFLFHDNLMPRLQRLGLISERVAPKYREIGLLN"
     gene            10853..>11073
                     /locus_tag="VLF19_09075"
     CDS             10853..>11073
                     /locus_tag="VLF19_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE93444.1"
                     /translation="MRYHQGVTGEPIYLKQMELGPMQNFVYLVGDPTTRECVVVDPAW
                     EIDTIVDTAAADGYTIKGALVTHTHQDHVG"
BASE COUNT         1585 a         3916 c         3887 g         1685 t
ORIGIN      
        1 agttcgtcct ctatccgggc gcgccccacg ccttcttctc ggacgaccgg ccgcaggtct
       61 acaagaagga agcggccgag gacggctgga agcgctgtct cgccttcttc gacaagcacc
      121 tgaaggcctg agtcaccgcg cgtaggcggg gaagacgcgg cggacgacgt cgggcgggtt
      181 cacgcgcgag agccacccgg cgagccgccg cgccgctccg cgcaccgcga cgaaggtgat
      241 gcgcgcgtcg gcgacgaggc ggccggcggc gtcccaggcc gtcacctcgg tcgcccagta
      301 gcgcggatcg tccgcgagcg ggcggacggc gtcgcggcag ccgccgacgg tcacgacgtc
      361 gccaaacggc accgggtcgt gcagggtcac cgacaggtcc gtggtcatcc ccgactcccc
      421 gctcgccagc gcccccagcc agaacgccgc ctcgtcgagc atcgtggtga gcgccaccgg
      481 cgcgagcgcc ccgttggccg cgcggaaggc cgggcccggc tgccagcgcc cggcgacggc
      541 ccggtcgtcg aacgagagcc ggagcctgag cccgatcggg ttgtccacgc cgcacgcgaa
      601 gcacgtcgtg gacacgggca ggggatctgc gggcagcgcg gccggcggcc ggccggtgcc
      661 cacgggagcg gcgacgggag cgatccgccc ctcgacgagc acggtctcgg aggtctccgc
      721 gagccgacac tcgagcgcat cgccgtcgcc cacgaccgtg agccggaggg gcacgccgag
      781 cggcacgcga cggcgataga cgccgcgcac ctcgcgcccg ccgacgcgcg cggcgagggc
      841 atcgaagacg gcgagcacgc tgccgccgtg cgcggtgtcc gggaggcccg agaacgcggg
      901 atccagcgtg aggatgcgct cgtcgccgcg cccctcgagc gcgcggcgca gcaggacgtc
      961 ggcgtccgac atgcgataat cgctcgtggc cattctagcg caccgcgcat tcgtgcgggc
     1021 cacggtcacc aacttcttct ttttcgcaag cctcaacggc ttcgtcctcc tgccgctcta
     1081 catcctgcgc ctcggcggca ccgaggtcga gatcggcctg gtgatggcgc tccacagcgg
     1141 cgtcggcatc gtctgccagc ccctgctcgg gccgtgggtc gacgcggcgg gccgccgacc
     1201 gttcatgctg ctcggtgtcg gcctggtcct gctgtccgcc ctcctcgcca ccgcggcgac
     1261 gtcgatcgcc tggctggcgg tcgtccgcgc gctgcaaggc atcggcttct ccgcgttctt
     1321 cgtcgcgaac tacacctacg tgatcgacct cgtccctgcc gagcgccgcg ggtgggcgct
     1381 cggcatctac ggggtgtcgg ggatgatctc gaccgcgctg acgccgctcg tgggcgaggc
     1441 gatcatccgc acgctcggct tccgcccgtt cttcgggttg tgcgcgctgc tggcggcggt
     1501 ctccggcgtg ctcgtgtggc agctcgccga gccggcgcgg gccgaggcgc cgacgacgcg
     1561 cgcgtgggag atcgcgcgcg ccggggtcgg cgacatgctg cagctccaca tggccgtgac
     1621 ggtcttcttc gggctgggca cgggcacggt gttcgtgttc ctgccgacgt tcgccgagga
     1681 cctcggcgtg accacgctcg cgctcttcta cacggcctac gcgatggcgg ccatgggcgt
     1741 ccgcgtcgtc gcgggcggcc tcatcgacac gcgcggccgt cgcgcggtga tcgtgccctc
     1801 gatgttcctg caggctgccg ccgccgccct gcttgccggg ctcggcgccg cggtcactcg
     1861 cacgagcccg gcaccggcgc tgcccgtgct catcgtcgcc gggctgatgt ccggcgctgc
     1921 ccacggcttc ctctatccgg gcctggccgc gctcgtcgcc gaccgggcgc cgcccgagcg
     1981 gcggggcgcc gtcgtcggtg tcttcagcgc cgtgttcctc gtgggcaacg ccggcggcgc
     2041 cttcgccttc ggctacgtcg cccacgcgtt cggctacgcc gcgatgtggg gactgctcgc
     2101 cgcgctgctg ctcgcgggcg cctcgctgag cctgcgcctc gcggaggaca cgcggctgcg
     2161 gccgctgcgc gccgccgagt gagccgaccg ccccgaccgg gcggtatact tgcgccgacg
     2221 aacgaggcgc ggccggccgg gccgggacgt gccgagcata ggggcgctgg gggccatgcc
     2281 ggggccgccc tcgccgacgg tgatcgaggg ccacggcgtt ttcggacgtg ccgaacgccg
     2341 gggggcgttg gccgtcctgt cgaggcccca tgaatcgccg gacggtgatc gaggcgcgcc
     2401 acggcgtggc cgggggtgcc gagcgttggg aggcttgggg ccctgtcggg gcccccatgt
     2461 aatcgaattc gcggaggttc ccatgagcac gacatgccgc ttcggaggcg tcgcgctgct
     2521 cgcactcgcg ctgacctgcg tggcgactcc aggcgacggc gcgcgcgacg ccgcgtcacc
     2581 gcccgcgccg cgaccgggcg ggccgcagga agccgaggtc gtcggtgtcg cggtggatcc
     2641 ccgcacccag gtccccgcgc tggtgctgca gggcaagcgt gacaagcgga acgtcgtcat
     2701 gtcgatcgat ccggcgcaga tgctggccat ctcgctgccg ctgcagggcg tcaccccccc
     2761 gcggccgctc acgcacgatc tcttcctctc gctcttcgcg cggctcaagg tgacgctgac
     2821 ccgcgtggtc atcaccgact tccgcgacga cgtctactac gcgctgctgc acctcagcag
     2881 cgacggcacc gacctgacgc tcgacgcacg gccctccgac gcggtcgcgc tcgccatccg
     2941 cgccaaggtg ccggtgctcg tggaggaccg cgtgttcgac cggggcggta tcgacaagcc
     3001 ggcgccgggt cgcccgtcga tctgagcgat ggccccggac accgacggcc tcgagtgggc
     3061 cctccagcgc cggctggagg acgtcttcag caagctcggc gagaagtttg gcgacctcac
     3121 cggccgcatc gagaccacga tccgcccgga cgtgtccttc gacgacgtcg gcgggctcgg
     3181 ggcggcgaag gaggtggtcc gcgggttcac ccacgcgctc gtcgaacccg accgttatcg
     3241 caactggggc atcgcgccgc ccaagggggt gctcctctac ggtccacccg gcaccggcaa
     3301 ggccatgctg gcccgcgcgc tcgccggcgc ctcggaggcc atcttcttcc gcctgctgct
     3361 ccacaatctg accgccaagt tcggcgcgaa cacggccgag ctgctgcagg aggcgctccg
     3421 catcgccgtc ggcgagggcc gcgccgtctt tttgctcgac caggtggacg cgctgtcgct
     3481 cgaccacctg ctgccgccgc cgcaggcgcg ggaggcgagc gcgcgcctgg tcgccgcgct
     3541 gtgcgagaag ctcgacgccc tcgatccggc ctcgcgcgtg ctcgtcgtgg ccgcgaccag
     3601 ccggaccgac gcgatcgacc ctgccctggt ggcgccgggc cggctcgacc acctcgtcga
     3661 gatccccctg ccagacgccg ccgcgcagca ggagatcctc gagctgatcc aggcgcgcat
     3721 ggagaagagc gccggccgcc cgctcttcgc gccgctcgac taccgcacgg tcctgccgct
     3781 gatgggcggc atgagcggcg ccgacatctc cgacatcctg ggccgcgcgc tggaagccaa
     3841 ggtgcaccgc gaggccgccg agggcaccgc gtcgcccgtc ggcaccgccg atctgctcga
     3901 ggccatcgac gcctacaagc gcgtgcgagg cgtcgtcgag aagatccgct acgggcagta
     3961 cctctagcgt ggagcgcccc cagaccgtca ccacgaccct ccgggtccgg tacaaggaca
     4021 cggactgcat gaaggtcgtc tactacggca actacctgac ctatttcgag gtcggccgcg
     4081 tggagtacct ccggcagcgg ggcatgccca tctcggaggt cgatcggcgc gtccacatgc
     4141 cggtggtcga ggcccacgtg cggtacgtcc ggcccgcgcg gctcgacgac ctgctcgaga
     4201 tccgctgctg ggtcagcgag cgccggcgcg cttcgttccg gttcgcctat gacgtgcgca
     4261 gcgaggccgg cgacaccatc gccagcggct ggacgctcca cgcctgctgg gacccggcca
     4321 cgtcccggat gatcgcgctg cccccctggc tctcggcgct gatgccggtg gctcccgaca
     4381 ccccggcgga gcgtgtataa tgtcgcctcg attgaaccgt ctcgtatgat cgaagtccag
     4441 aacctcacga aacattacgg tccggtcacg gcgattcgcg acgtgtcgtt cgcggtcgcg
     4501 ccgggcgcga tcgtcgggtt cctgggcccc aacggcgccg gcaagtcgac gacgatgcgc
     4561 atcctgtcct gcttcatgcc cgcctcgagc ggcaccgcgc gcgtcgccgg ctacgacgtc
     4621 ttcaaggagt cgatggaggt ccgcaggcgg atcggctacc tgccggagag cgtgccactc
     4681 tacgcagaca tgcgcgtggc cccctacctc gatttcgtcg ccgaggtgaa gggtctcggg
     4741 cgcgccgacc gccggcgtcg ggtcggcgag gtgatggagc gctgcctcat cgccgacgtg
     4801 cagaaccgcc tcgtcggcaa gctctccaag ggctaccggc agcgcgtcgg cctggcccag
     4861 gcgatcatca gcgatccgca cgtgctgatc ctggacgagc ccaccatcgg gctcgacccc
     4921 cgccagatca ccgagatccg ctcgctgatc aagtcgctcg cgggcgagca taccgtgatc
     4981 ctgtccaccc acatcctgcc cgaggtgtcg atggtgtgca gcggcgtcat catcatcaac
     5041 aagggctcga tcgtcgccca gggcccgatc gacaccctcg tcgagcagtt cttccccacc
     5101 gcccgggtgc aggtggagat ccgagggccg gcggcggccg tgcgggaggg catgcagcgc
     5161 ctgcccgggg tgctcgaggt gcgcgagcag gaggtcatgg acggccacgg ggtctacatc
     5221 gtggagtcct cccgcgaccg tgacgtccgc gcggagatct tccagctggc cgcccagcag
     5281 aagtgggagc tgcgcgagtt gcgacgcgtg ggcatgacgc tcgaggaggt cttcatccgc
     5341 atcgtggccg gcgaggaaac cgacgagcgc gcggcgccgg cggaggccgc atcgtgaagg
     5401 tctggccgat cttcaagaag gagatgcgcc tctacttcac gtctcccatc gcctgggtga
     5461 tcctgacgat cttcctcctc atctccggct acttctttta caacatcttc gccttcttca
     5521 cgatggcctc gatgcagtcg gcgatgaacc cggccatggc ccgcgagctc aacgtgacgg
     5581 acagcgtgat gcgtccgctg ttctccaaca tcagcgtcat cctcctgctg ctcatgccgc
     5641 tggtcaccat gcggctcttc gcggaggagc gccgcgccgg cacgatcgag ctgctgctca
     5701 cgtacccggt ccgggacggt gccgtgctca tcggcaagta cctggccgcc ctggcgctct
     5761 acgccgtcat gctggtgctg acgctcgtct accccgcgat ggtggtgtac ttcgcgcggc
     5821 tcgagtgggg cgtcctgctc acgggctacc tggggctgct cctcatgggc gccaccttcc
     5881 tggccgtggg catcttcgcc tcgtcgctga ccgagaacca gatcgtggcg gccatcgtca
     5941 cgttcggggt cctgctgatc ttctgggtga tcggctggtc ggccgactac gcgggcgggc
     6001 cgtggagccg cgtgctgtcg cacgtctcgc tgctcgagca cttcgactcg ttcgccaagg
     6061 gcgtgctcga cacgaaggac atcatctact acctgaactt cacgctgctg gcgctgttcc
     6121 tgacgctccg gtcactggag gcgcggcggt ggaaagggta gcacggacgt gaggcggctc
     6181 ttcgactggg cgggttacgt cgggctcgcc gccctggcgg cgctggcgat cctcaactgg
     6241 acccggccgc ccttcatgac gggcacgcgc gccacgtgga tctggtggtc gctgctcgtg
     6301 ggcggcgtgc tgctggtgct gatctcgctg ctcgcctacg cgcggggcag cggcgtcttc
     6361 ggcggccgga cggcgcgcta cgggctcaac acggccctcg cggtggtcct gctgctggcg
     6421 atcgtcggcc tcgtcgaggc cgtctcgtac cgccacaacg cgcggctgga cctcaccgag
     6481 aacaagcgcc acagcctctc gccacagacc atccagctgc tgcgcgatct caagaccgac
     6541 gtgaacgcgg tggcgttctt ccggagcgac cagcccaaca agcgcgccgc cgaggacctc
     6601 ttgaagcagt accagcgcta cgccggtagc aagctcacgt accgcgtggt ggacccggac
     6661 cgcgaaccgg ggctggcacg ccgttacgcc gtcgagtcct acggcaccgt cgtgctggag
     6721 acgaaggacc gctccgagaa ggtgctcgac gccgaggagg agaagctcac caacggcctc
     6781 gtgaagctca cccgcgaggg caagcgagtc gtctacgtgg tgcagggcca cggcgagcgc
     6841 gacctcgcca gcaccgaccg gcccggcttc ggcgaggcca agacggcgat ggagcgcgcg
     6901 aactacgagg tgaagccgct cgccctcgcg cgccaggaca agatcccgga ggacgcctcg
     6961 gtcgtgatcg tggccggatc gcggacggag cttctgccgc ccgagctggg cgcgctcgag
     7021 gcctacgtcg gtcgcaacgg caagctcttc gtcatggtcg atccgggcat catcggcggc
     7081 gttcaggccg agggcctcct gcgcttcctc ggcaagtatg gcttcgagct tggcaacaac
     7141 ctcgtcatcg agctgaaccc catcgggcgc ctgttcggca tcgggcccga ggtgccgatc
     7201 atccagcagt acgagaccca tgccatcacg cgcgacatgg cgggcatcag caccctgttt
     7261 ccgctgacgc ggacggtcga ggtggccaag gcgcccccgg cagggatcgt gctgacgccg
     7321 ctcgcccgga cgagcaccga gagctgggcc gagacggacc gggcgtcgct ccaggcaggc
     7381 caggtgaagg ccgatcccca ggacctgaag ggcccggtca ccgtggctgc cgtcgccacc
     7441 aaggacaagg cccgcatcgt cgtctacggc acctcgagcc tggccgccaa ccagttcctg
     7501 aacctgcagg gcaaccgcga tttctttctg aacacggtct cgtggctggc cgagcaggag
     7561 gatctcatcg ccatccgccc gaaggatgcc cgccagaccc cggtgttcat gacctcccgg
     7621 caggcgcagc tcgcgttcct ggtgcccgtc gtcgtgctcc cgggagtcgt gctcgccggc
     7681 ggcatcttca ccctcgtcag gcggcgggcc gcccggtagc tcccgagccg gcgaagtgat
     7741 cgctcacgtc tctccgaagg ctgatggctc gagcgccggc ctcgccgggg ccctcgatcc
     7801 tgctggaggt tccgaagacg gccgtcgagc cgccgtccga ctgttgccat gagatggcag
     7861 acgacggcgt tcctggcgat cctcctgatc gcggtcggta ccttctacta tatgtacgag
     7921 gttcgctggg cccccgaccg ggagcaggcg cagagccgca agggccgggt gttcgacgtg
     7981 gacgccgccg acgtgaccga gctgacgctc aagcgaggcg acgaggtcgt gcggctggtg
     8041 cgcgagagcg acggctggca gatgacggcg cccctcgcct ggcgcggcga gcgtgggccc
     8101 atcgaggaga cgctgacgtc ggtcgcgacc gcccggatgg accgcgagat cgccgcggtg
     8161 ccggccgacg tcgccgagtt cgggctcgac aagcccgccg ccgaggtgac gctcgcgacg
     8221 aagggcggca agcagctcgg actccggctc ggcgccaagt cgccgaccgg cgtctgggtc
     8281 tacgcgcagg agcgcggaaa gccggcggtc ttcgtggtcg gggacggcgt gctgcgggac
     8341 gccacccggc cgctggccga cttccggagc aaggccgtgc tcagcttcga ccgcaaggac
     8401 gtcaccggcg tcgagatcgt cggccgcgac gagacgctgg cggtcgagcc ggcgggcgag
     8461 cgtggctggc gcatgacgcg gccgcgcgcg ctcgaggccg accccgacgc cgtcgtcgag
     8521 ctgctggaga agctggcggg tgcgaggatc agggaatttg tcgccgagac gccgcgctcg
     8581 cttgcgccct acggcctcga gcggccggtc cgggtggcga tccacgtcgg caaggacaag
     8641 gaccgcgcga cccggacgat cctgctcggc aacacggacg acaagaagaa gggcgtgtac
     8701 gcgctgcggc cgggcgagac gacggtgctg ctgctcccgg aggacgtctc gaaggccgtg
     8761 ccccggacga ccgccgctct tcgcaacaag acggtgatcc cgatcgagcg cgacaaggtc
     8821 ggcgggctcg aggtcgagag cccgcgcggc gccgtgacgc tcgtccgcga gcaggacaag
     8881 tggcgcatca cgcagccgga ggcgctgccc gccgatcagg tcgaggccgg cgccgtcctg
     8941 atgcggctcg tgaacctcaa ggcgctcgcc ttcctgagcg aggacgcgtc ggcgatcccg
     9001 cggtatctcg ccaagcccga ggtgcgggtg acgatcggcc agcagggcga ggcttccagc
     9061 cagaccctcc tgctggcgcc gtcaccggag aaacgcggcg gccaggccac ggcgtacgcc
     9121 gccgtcgccg gccgaggccc cgtggtcctc gtcgacgcct ccgcgctgac cgagatcggc
     9181 cggccgctgc ggcagctccg cgaccggacg ctggtcgccg ggctcgagcc acgcgacgtc
     9241 aagcgggtcc acgtcaagcg cgacggcaag gcggtgctcg tcgagcgcac gggcgacacc
     9301 gagtggcggg tcctcgaggg cggcaaaggc acggcgaaca ccgcgaaggt ggaggatctc
     9361 ctctacgggc tgcgtaacct caagtggcag gacatcgccg cgcccgacgg cggcgaagcc
     9421 gcgaagttcg ggctcgccgc tccggcggcc gaggtcacgc tctaccgcgc ggacggcacc
     9481 gccatcgcca ccttgctcgt cggggggcag gagggcgacc ggcgctacgt ccagatgaag
     9541 gccgcgccca ccgtctacgc gatcgacgcc aaacagctga cgctgccgaa ggttcccgaa
     9601 gacttccaga gctgacgtct cgaatctgga acccgcggtc gaggcgccgg cccggcctcc
     9661 ggtcggtggg ctttcgacac gctcggagcc tgccgagcgt cggaggtttg gggcccgttc
     9721 ggggccgcca cggctgtgcc ggggcaggcc gagccgcccg gggggcctgg ggagcctgtg
     9781 ccgggcccta gacggtcaat tcaggaggcc gatctcgcgg tacttcgggg cgacgcgctc
     9841 ggagatgagc ccgagacgct gcaggcgcgg catcaggttg tcgtggaaga ggaacgagtt
     9901 gaagaccttg gtcgtgggcg acgtcgccac ggtctcccgc cagatcttct cgccgtccca
     9961 gcccatctcg ttccacacgt tctggatgga cttgaagcca gtgcgctcgt cgcgagcgta
    10021 catgaggtcg cacgccttca ggcagaagtc ctcgaggtag gcgcgcttga ccgggtccag
    10081 atctgggatc gagcggcgca acgcgaagta gccgaagttg acgtggcgcc cctcgtcttg
    10141 gagcacgtag tcgaggagct gggagagggt ggggtccgcc gtctgcttgc gcatgacgtt
    10201 gaaggccgcg atggcgaggc cctcgatcat catttgcatg cccacgagct tcagctccca
    10261 gaggttcgtg gcgaggatct cgtcgatgag ggccttgagc agcgggtcca cggggtagat
    10321 cttgtggagc ttcttgacgt acttctcgaa gacctccacg tgccggccct cgtcgaccac
    10381 ctgggtggcg gcgtagagct tcgcgtccag ctcggggatc gagttcacga gctggccgca
    10441 gacgacgagc gcgccctgct cgccgtgcag aaactggctg agccgccacg cggagacgcg
    10501 gatctcgacc tccttctgct cctccggcgt cagccggctg aagaagttcg tgccaccgaa
    10561 ggcgagctgc acgttgagga cgggaccctc ggtgcccacg ggccggctcc agtcgatagc
    10621 ggtgctcccg ttccactggt tcttcttggc ggtctcgtag agggttgcca gctcctcgtg
    10681 cgtgggcacg taatcgtagt cccagagcgt ttcgagctgg taagtcacgc gccgggccgc
    10741 ctgctgcgcc gtcgtcatcg ctgtcacctc gcgccgtgca ccggagggtg gtgtatcgat
    10801 gagcatactg aaccaccgtt catcggggcc cagtctagcg cacccccgac cggtgcgcta
    10861 ccatcagggc gtgaccgggg agccgattta cctgaagcag atggagctgg ggccgatgca
    10921 gaacttcgtc tacctggtcg gcgacccgac cacccgcgag tgcgtggtgg tggacccggc
    10981 gtgggagatc gacaccatcg tcgacaccgc cgcggcagac ggctacacca tcaagggcgc
    11041 gctcgtgacg cacacccatc aggaccacgt ggg
//
LOCUS       DATIAZ010000252         4999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1120870, whole genome shotgun sequence.
ACCESSION   DATIAZ010000252 DATIAZ010000000
VERSION     DATIAZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120870"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VLF19_09080"
     CDS             <1..870
                     /locus_tag="VLF19_09080"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSE93445.1"
                     /translation="PSVTTTVTGAPTAAVQRVLTAVVFLPPFLWMVSRGPGWVFVLFV
                     MAVSGLALWELLRLFQGAGWPVYTRTAIALGVVVTGSFALPAPAVVPSAFLCLAAAVL
                     LSAPLWSGTPPQAEPAALTLVSLVYVSWFLGHVLALHRRPEGDFLVLFLVTVTWLGET
                     AAYVVGSTVGRHRLAPAVSPRKTIEGAVAQLVASVAGAGLFAAWLLPAWSLAGALVAG
                     SILGVVGQVGDLVESVMKRSVGVKDAGGLIPGHGGVLDRIDGLLFNTPALYYYVTLGG
                     ATLGGAPALAASS"
     gene            867..2033
                     /locus_tag="VLF19_09085"
     CDS             867..2033
                     /locus_tag="VLF19_09085"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSE93446.1"
                     /translation="MKRLTLLGATGSIGLRTLDLVSSFADEFCLAGLAARGSNVELIA
                     DLCRKHTPRAVALLDETAVDRLAGLLPSPRPELLAGAPGLVALTRDVEADMVVSALVG
                     GAGLLPTMAAIEAGRPVALANKETLVMAGALMTAAARRRGVPLLPVDSEHSAIFQCLV
                     GHDRGAVHRILLTASGGPFRELPKERFAHVTVEDALRHPTWKMGAKITIDSATLMNKG
                     FEVIEARWLFDLEPHRVQVLVHPQSIVHSMVEYIDGSVIAQLGVPDMGVPILYALTYP
                     ERRPAPAPRLDLTRTGPLTFFPPDEARFPCLGLARAALERGGAAPVVLNAANEVAVAA
                     FLERKIGFARIPELIGRALDAMASDACSSIDDCVAIDADARRRAQALVTAHGGE"
     gene            2038..3402
                     /gene="rseP"
                     /locus_tag="VLF19_09090"
     CDS             2038..3402
                     /gene="rseP"
                     /locus_tag="VLF19_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HSE93447.1"
                     /translation="MTTILSFVVVIGILILIHELGHFLVARWTGVGVERFSMGFGPVL
                     LRWRGKETEYCLSAIPMGGYVKMMGEENPLEGGGNLAYDPTKAFSLKPLWARFLIVFA
                     GPGMNFVLAAVIFMIVLATIGRPVWPAVIGRVAEASPAAAAGLHTGDVVVAAQGRPVE
                     HWEDLERVVAASEGRPINLRVRRGDAESTITVSPRQTTLRDPIFKDAREVWESGAGPK
                     LTPQIGAVTPDSPAERAGVKPSDQVVAVAGQPVFTPEELMQAIQKRGGQTFDITVERE
                     GRRLALSVTANVVKEKSPTGQEVEVGRIGVSIVTRTVNYAAYSPPQAVWYGLVRTWDM
                     TVLTIRGFWKIVTGQIALSNLGGPVQIASETGRQAQEGVAPLALFTAVISVNLAVLNL
                     LPVPMLDGGHLLFFLIEGVLGRPLSVRKRELAQQVGFVLLMLIMVLALYNDLVRIDAF
                     RFFK"
     gene            3413..4510
                     /gene="ispG"
                     /locus_tag="VLF19_09095"
                     /gene_synonym="gcpE"
     CDS             3413..4510
                     /gene="ispG"
                     /locus_tag="VLF19_09095"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSE93448.1"
                     /translation="MITRRKTRQIRLGPVTVGGGAPITVQSMTKTDTRDVQATLLEIW
                     SLEAAGCDIVRCAVPVREAAEKLGEIKRQIRIPLVADIHFNYKLALIALEQGVDGLRL
                     NPGNIGGKKFVLEVVALAKERKIPIRIGVNAGSLEKDLLEKHGGPTAEGMVESALRHI
                     RILEDCNYPEMKVSLKASDPLMMIEAYRMLAGQVDYPFHLGVTEAGTPGVGTIKSAVG
                     LGALLSEGIGDTIRVSLSADPTEEVRVGVDVLKALGLRKGGLTFVACPSCGRADVDLI
                     KLAREVEDEFRGLNEEIHIAVMGCEVNGPGEARAADIGVAGGRGIGLIFKNGEVIRKV
                     PESEIVRAMREEVDRFLADRRTERAAAGSAD"
     gene            4510..4842
                     /locus_tag="VLF19_09100"
     CDS             4510..4842
                     /locus_tag="VLF19_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HSE93449.1"
                     /translation="MGQYRTHVFVCTSGETCPTQGDVESYVKWLRGECAKAGLKTEVR
                     VNKAGCFSQCGHGPMLVVYPDDVWYAGVQQSDLREILESHILGGMPVERLRYAPGVKG
                     ANKTPDAH"
BASE COUNT          684 a         1749 c         1788 g          778 t
ORIGIN      
        1 ccgtcggtca ccacgaccgt cacgggcgcg ccgaccgcgg cggtccagcg ggtgctcacg
       61 gccgtggtgt tcctgccgcc cttcctgtgg atggtctcgc gcgggcccgg atgggtgttc
      121 gtgctcttcg tgatggccgt gagcgggctc gcgctctggg aactgctgcg gctcttccag
      181 ggagccggct ggcccgtgta cacgcggacg gccatcgcgc tgggcgtcgt ggtgacgggc
      241 agcttcgcgc tgccggcccc cgccgtggtt ccgagcgcgt tcctctgcct ggccgcggcc
      301 gtcctgctga gcgcgccgct ctggagcggc acgccgcctc aggccgagcc ggcggccctc
      361 acactggtgt cgctcgtcta cgtgagctgg ttcctcggcc acgtgctcgc gctgcaccgg
      421 cggccggaag gcgacttcct cgtgctcttc ctcgtcaccg tcacgtggct gggcgagacc
      481 gcggcgtatg tcgtgggctc caccgtcggg cgccacaggc tcgcgcccgc ggtcagcccg
      541 cggaagacca tcgagggcgc cgtcgcccag ctcgtggcct ccgtcgccgg cgccgggctc
      601 ttcgccgcct ggctactgcc cgcgtggtct ctcgccggcg ccctcgtcgc gggctcgatc
      661 ctcggcgtcg tcgggcaggt gggcgacctc gtcgagtcgg tgatgaagcg gtcggtcggc
      721 gtcaaggacg ccggcgggct cattcccggt cacggcggcg tcctcgaccg catcgacggg
      781 ctcctcttca acacgccggc cctttactat tacgtcacgc tcggcggcgc gacgctcggc
      841 ggcgcgccgg ccctggcggc gtcctcgtga agcgactcac gctgctcggc gccaccggct
      901 ccatcggcct gcgcacgctc gacctcgtct cgagcttcgc cgacgagttc tgtctggccg
      961 gtctggccgc ccgcggctcg aacgtcgagc tgatcgccga cctctgccgc aagcacaccc
     1021 cgcgcgcggt ggcgctgctg gacgagacgg ccgtggaccg cctggccggc ttgctgccat
     1081 cgcccagacc cgagctactc gcgggcgcgc ctggcctggt ggcgctgacg cgggacgtcg
     1141 aggccgacat ggtggtctcc gcgctcgtcg gcggggccgg tctgctgccc acgatggcgg
     1201 ccatcgaggc ggggcggccg gtggcgctgg ccaacaagga gacgctcgtg atggccggcg
     1261 cgctcatgac cgcggcggcg cgccgacggg gcgtgccgct tctgcccgtg gactccgagc
     1321 acagcgcgat ctttcagtgc ctcgttggcc acgaccgcgg cgcggtccac cgcatattgc
     1381 tgacggcgtc gggcggcccc ttccgcgagt tgccgaagga gcgcttcgcg cacgtcaccg
     1441 tggaggacgc gctccgccac ccgacctgga agatgggcgc gaagatcacg atcgactcgg
     1501 cgacgctcat gaacaagggc ttcgaggtga tcgaggcgcg ctggctcttc gacctcgagc
     1561 cccaccgggt gcaggtgctc gtccacccgc agtcgatcgt gcactcgatg gtcgagtaca
     1621 tcgacggttc cgtcatcgcc cagctcggcg tccccgacat gggcgtgccc atcctctacg
     1681 cgctcacgta tcccgagcgg cggccggcgc cggcgccgcg gctggacctc acgcgcacgg
     1741 gaccgctgac cttctttccg cccgacgagg cgcgcttccc gtgtctcggc ctggcgcgcg
     1801 ccgcgctcga gcgaggcggg gcggccccgg tggtgctcaa cgccgccaac gaggtggcgg
     1861 tggcggcctt cctcgagcgg aagatcggat tcgcacggat tcccgagctg atcgggcgcg
     1921 cgctcgacgc catggcctcg gacgcgtgct cgtcgatcga tgactgcgtg gcgatcgacg
     1981 ccgacgcgcg ccggcgcgcc caggccctgg tcaccgcgca cggaggcgag tagtcacgtg
     2041 accacgattc tctcgttcgt cgtcgtcatc ggcattctga tcctcatcca cgagttgggc
     2101 cacttcctcg tggcgcggtg gacgggggtg ggcgtcgagc gcttctccat gggcttcggc
     2161 cccgtgctgc tgcgctggcg gggcaaggag accgagtact gtctctccgc catcccgatg
     2221 ggcggatacg tgaagatgat gggggaggag aacccgctgg agggcggcgg caatctcgcc
     2281 tacgatccga ccaaggcctt ctcgctcaag ccgctctggg cgcggttcct gatcgtcttc
     2341 gcgggcccag gcatgaactt cgtgctcgcc gccgtgatct tcatgatcgt cctggccacc
     2401 atcggccgtc ccgtctggcc cgccgtcatc ggccgggtcg ccgaggcctc gccggcggcg
     2461 gccgccggcc tgcacacggg cgacgtggtc gtcgccgcgc agggccggcc cgtcgagcat
     2521 tgggaggacc tcgagcgcgt ggtcgccgcc tccgagggcc ggcccatcaa cctgcgcgtg
     2581 cgccgcggcg acgccgagtc gacgatcacg gtgtcgccac gccagaccac gctccgcgac
     2641 ccgatcttca aggacgcccg ggaggtgtgg gagagcgggg cgggcccgaa gctgacgccg
     2701 cagatcgggg cggtgacgcc ggactcccct gccgagcgcg cgggagtgaa gcccagcgac
     2761 caggtcgtcg ccgtcgccgg gcagcccgtg ttcacgcccg aggagctgat gcaggccatc
     2821 cagaagcgcg gcgggcagac gttcgacatc accgtcgagc gagagggccg ccgcctcgcg
     2881 ctgtccgtca ccgccaacgt ggtgaaggag aagagcccga cggggcagga ggtcgaggtc
     2941 gggcgcatcg gggtgtcgat cgtcacgcgg acggtgaact acgcggcgta ctcgccgccg
     3001 caggccgtgt ggtacgggct cgtgaggacg tgggacatga ccgtcctgac gatccggggc
     3061 ttctggaaga tcgtcaccgg gcagatcgcg ttgtcgaatc tcggcgggcc cgtgcagatc
     3121 gcgtccgaga cgggccggca ggcccaggag ggcgtggcgc cgctggcgct cttcacggcg
     3181 gtcatcagcg tcaacctcgc cgtgctcaac ctgctgccgg tgccgatgct cgacggaggg
     3241 cacctgctct tcttcctcat cgagggcgtc ctcggccgcc cgctgtccgt gcgcaagcgc
     3301 gagctcgcgc agcaggtggg cttcgtcctg ctgatgctga tcatggtgct ggcgctctac
     3361 aatgacctgg tgcggatcga cgcgttccgg ttcttcaagt gaggcgcggg gcatgatcac
     3421 acggcgcaag acccggcaga tccggctcgg cccggtcacg gtgggcggcg gcgcgccgat
     3481 caccgtgcag tcgatgacca agaccgacac ccgagacgtg caggccacgc tcctcgagat
     3541 ctggtcactg gaagcggcgg gctgcgacat cgtccgctgc gcggtgcccg tgcgcgaggc
     3601 cgccgagaag ctcggcgaga tcaagcggca gatccgcatc ccgctcgtcg ccgacatcca
     3661 cttcaactac aagctggccc tgatcgcgct ggagcagggc gtcgacggcc tgcgcctgaa
     3721 ccccggcaac atcggcggca agaagttcgt cctcgaggtg gtagccctcg ccaaggagcg
     3781 gaagatcccc atccgcatcg gcgtcaacgc cggatcgctg gagaaggacc tgctggagaa
     3841 gcacggcggg ccgaccgccg agggcatggt cgagtcggcg ttgcgacaca tccggatcct
     3901 cgaggactgc aactacccgg agatgaaggt gtcgctcaag gcctcggacc cgctgatgat
     3961 gatcgaggcc taccgcatgc tcgccggcca ggtggattat ccgttccacc tcggcgtgac
     4021 cgaggccggg acgcccggcg tagggacgat caagtccgcg gtcggcctgg gcgcgctgct
     4081 ctccgagggc atcggtgaca cgatccgggt gtcgctgtcg gcggacccga ccgaggaggt
     4141 gcgcgtcggc gtcgacgtcc tcaaggcgct ggggctgcgc aagggcggcc tcacgttcgt
     4201 tgcgtgcccc tcgtgcggtc gcgccgacgt cgacctcatc aagctcgccc gcgaggtcga
     4261 ggacgagttc aggggtctca acgaggagat ccacatcgcc gtgatgggat gtgaggtgaa
     4321 cgggcccggc gaggcgcgtg ccgcggacat cggcgtcgcc ggggggcgtg ggatcgggct
     4381 gatcttcaag aacggcgagg tcatccgcaa ggtcccggag agcgagatcg tgcgagcgat
     4441 gcgcgaggaa gtcgaccggt tcctggccga ccgccgcacc gagcgtgcgg ccgccgggag
     4501 cgcagactga tggggcaata ccgcacgcac gtgttcgtct gtaccagcgg cgagacctgc
     4561 cccacgcagg gcgacgtcga gtcgtacgtg aagtggctgc gcggcgagtg cgccaaggcc
     4621 gggctcaaga ccgaggtccg ggtcaacaag gccggctgct tctcgcagtg cggccacggc
     4681 ccgatgctgg tcgtctatcc cgacgacgtc tggtacgccg gcgtgcagca gtcggacctt
     4741 cgagagattc tcgagtcgca catcctcggt ggcatgccgg tggagcgcct ccgctacgcg
     4801 cctggcgtca agggcgccaa caagacgccg gatgcccact gatgacatga gggcgcgaag
     4861 atggcccccg agcccccaac gccgggcacg tccatgccgc gccgtggcgc gcctcttcaa
     4921 ccccacaggc gcctagacta gccggatgcg gctgcgccag tcgctcgtcc ccacgctccg
     4981 ggaagatcct gccgaggcc
//
LOCUS       DATIAZ010000253         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1384950, whole genome shotgun sequence.
ACCESSION   DATIAZ010000253 DATIAZ010000000
VERSION     DATIAZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384950"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VLF19_09105"
     CDS             complement(<1..294)
                     /locus_tag="VLF19_09105"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HSE93450.1"
                     /translation="MAGRDTAPHAGDGALAPGSRILDRVALAVATAGGAGYSPVVPGT
                     VGSAVGAAILWLVPFSRAGLALFFVAVTLAGIWAAHRAERLLRAKDPGAIVIDE"
     gene            complement(299..1612)
                     /gene="rimO"
                     /locus_tag="VLF19_09110"
     CDS             complement(299..1612)
                     /gene="rimO"
                     /locus_tag="VLF19_09110"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSE93451.1"
                     /translation="MKVHLTTLGCPKNQVDSELMLGMLAEAGFPLVEHAEEADCLIVN
                     TCAFIDRAREESVQTILELAQLKERGRATSLIVTGCLTQRYGGHLRREMPEIDGMLGT
                     SDLHRIVDLVGQAHNRRDWATAAPPGYLYDATTPRLLAARVPYAYVKIAEGCDMGCTF
                     CAIPQFRGRHRSRPLGDVVKEVEALAARGIQEAILVSQDTLAYGRDLPGNGEIADLLD
                     ALSDTPMPWIRPMYLHPAHVNDRLIERWSRARVVPYLDMPVQHGDDGVLRAMRRAVTA
                     RRMKEIVAAFRRAIPGVTVRTTVLVGFPGESEAAVASLLGFVEEVGFDRLGVFTYSPE
                     EGTPSVDYPDRVPSDVAAERAAAVQETQDRVAWERQRALVGSVQEVLVDGPSEDPAFA
                     WEARTAGQAPEIDGVVYLRGRFEPGRFARVRIVEVEGYELVGERV"
     gene            complement(1612..2109)
                     /locus_tag="VLF19_09115"
     CDS             complement(1612..2109)
                     /locus_tag="VLF19_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HSE93452.1"
                     /translation="MAAENSFDVACKIDMQEVTNALDQARREIETRYDLKGSRNEVAL
                     EKTDIVLTAADDMKLKAVTDIVQGRLHRRGVPLKALTYGKVEEASGGLKRQRITLQQG
                     IPIEKAREIVRLVKDTKLKVQAAIQEDQVRVSGKNRDDLQKIIALLKDKDLGIALQFT
                     NYRSV"
     gene            complement(2139..>2585)
                     /locus_tag="VLF19_09120"
     CDS             complement(2139..>2585)
                     /locus_tag="VLF19_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSE93453.1"
                     /translation="GKLRWEFAEPAKQEIVSDGATLWVYTPSLNQVNTGPAPEALAGP
                     AGSFLAGLGRLREHFNVRFLNPAQPVDGDGHVVLDLTPKRPLPTMARLILAVDPKSWE
                     VRRAVVHDQFENTVTMRFTQMALNTGLADRLFTFVPPPGVATVPLR"
BASE COUNT          360 a          904 c          936 g          385 t
ORIGIN      
        1 ctcgtcgatg acgatcgcgc cgggatcctt cgcccggagc aggcgctccg cgcggtgcgc
       61 cgcccagatg ccggcgagcg tgaccgcgac gaagaagagc gcgaggccgg cgcgcgagaa
      121 cggcaccagc cacaggatcg ccgcgccgac cgcgctgccg acggtgcccg gcaccaccgg
      181 cgagtagcct gccccgccgg cggtggcgac ggcgagtgcg acgcggtcga ggatacgcga
      241 gcccggcgca agggcgccgt caccggcgtg tggggccgtg tcgcggcccg ccacgtggtc
      301 agactcgctc gccgacgagc tcgtagccct cgacctcgac gatgcggacg cgcgcgaagc
      361 ggccgggctc gaagcgcccg cggaggtaga cgacgccgtc gatctccggt gcctgtcccg
      421 cggtccgcgc ctcccaggcg aaggccggat cctcgctcgg accgtccacc agcacctcct
      481 gcaccgagcc cacgagcgcc cgctggcgct cccaggcgac gcgatcctgg gtttcctgga
      541 cggcagcggc acgctcggcc gcgacgtcgc tgggcacccg atcgggatag tcgacggacg
      601 gcgtgccctc ctcgggcgag taggtgaaga cgccgagacg gtcgaagccg acctcctcga
      661 cgaagccgag caggctggcg acggccgcct cgctctcgcc cgggaagccc acgagcacgg
      721 tcgtccgcac ggtcacgccg ggaatcgccc gccggaacgc cgcgacgatc tccttcatgc
      781 gccgcgcggt gaccgcgcgc cgcatggcgc gcaggacccc gtcgtcgccg tgctggaccg
      841 gcatgtcgag gtacggcacc acgcgcgcac ggctccaccg ctcgatcagc cggtcgttga
      901 cgtgcgcggg atgcaagtac atcggccgga tccacggcat gggggtgtcg gacagcgcgt
      961 cgagcaggtc ggcgatctcg ccgttgccgg gcaggtcgcg cccgtaggcc agcgtgtcct
     1021 gggagaccag gatcgcctcc tggatcccgc gcgcggccag cgcctcgacc tccttgacga
     1081 cgtcgcccag cggccggctc cggtggcggc cgcggaactg ggggatcgcg cagaacgtgc
     1141 accccatgtc gcagccctcg gcgatcttca cgtacgcgta gggcacgcgc gcggccagca
     1201 ggcgcggcgt cgtcgcgtcg tagaggtagc ccggcggcgc cgccgtcgcc cagtcgcggc
     1261 ggttgtgcgc ctgaccgacg aggtccacga tgcggtgcag gtcggaggtc ccgagcatgc
     1321 cgtcgatctc gggcatctcg cgccggaggt ggccgccgta gcgctgggtg aggcagccgg
     1381 tgacgatgag cgaggtggcc cgcccgcgct ccttgagctg ggccagctcg aggatggtct
     1441 gcaccgactc ctcgcgcgcg cggtcgatga aggcgcaggt gttgacgatc aggcagtcgg
     1501 cctcctcggc gtgctcgacg aggggaaacc cggcctcggc cagcatgccg agcatcagct
     1561 cggagtccac ctggttcttg gggcagccga gggtggtgag gtggaccttc atcagacgct
     1621 gcggtagttg gtgaactgca gcgcgatgcc caggtccttg tccttcagca gggcgatgat
     1681 cttctggagg tcgtcgcgat tcttgccgct gacgcgcacc tggtcctcct ggatcgcggc
     1741 ctgcaccttc agcttggtgt ccttgaccag ccggacgatc tccctggcct tttcgatcgg
     1801 gatgccctgc tgcagggtga tgcgctggcg cttgaggccg ccggaggcct cctcgacctt
     1861 gccgtacgtc agcgccttga gcggcacgcc gcggcggtgg agccgcccct ggacgatgtc
     1921 cgtcacggcc ttgagcttca tgtcgtcggc cgcggtgagg acgatgtccg tcttctccag
     1981 cgccacctcg ttccggctgc ccttcaggtc gtagcgggtc tcgatctcgc gccgcgcctg
     2041 gtcgagcgcg ttggtgacct cctgcatgtc gatcttgcaa gcgacgtcga acgagttctc
     2101 ggcggccatg actgacagtc taccgcagga ggacggttct accgcagtgg gacggtcgcc
     2161 acgccggggg gcggcacgaa ggtgaacagc cggtcggcga gccccgtgtt cagcgccatt
     2221 tgcgtgaagc gcatggtcac cgtgttctcg aactggtcgt ggacgacggc gcggcgcacc
     2281 tcccacgact tcgggtcgac ggcgaggatc aaccgcgcca tcgtgggcag cggccgcttc
     2341 ggcgtgaggt cgagcacgac gtggccgtcg ccgtccacgg gctgggcggg gttcaagaag
     2401 cggacgttga agtgctcgcg cagccggccg agacccgcga ggaacgagcc agccggaccg
     2461 gccagcgcct ccggcgccgg gccggtgttc acctggttga gcgacggcgt gtagacccag
     2521 agggtcgcac cgtcggagac gatctcctgc ttcgccggct cggcgaactc ccagcggagc
     2581 ttgcc
//
LOCUS       DATIAZ010000254         6348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_69433, whole genome shotgun sequence.
ACCESSION   DATIAZ010000254 DATIAZ010000000
VERSION     DATIAZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69433"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VLF19_09125"
     CDS             complement(<1..1012)
                     /locus_tag="VLF19_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE93454.1"
                     /translation="MDYGLFTMPSHPPERSLYDGHQWDLQTLRWADELGFSEAWIGEH
                     HTAPWEPHPSPDLLVAQALLQTERMRIGPGGFLMPYHHPAELANRVAMLDHLAQGRLN
                     FGVAASGLPSDWAMFNVDGNAGQHREMTREALDIILRLWSDEPEFDHKGKYWHVTKTG
                     TMLDTLKPHIKPFQKPHPPIGVAGVSKGSDTLKLAGERGFWPMSLNLNPTYVGSHWES
                     VEIGAARSGRTPRRAEWRLVREIFVADTDAEAMRLSAGGMMGRMMKEYFLPLLASFQF
                     TEFLKHSPDVPDSDVTPEYCARHNWLVGSPATVAEKLHAIYEEVGGFGTLLLFCFDYV
                     ENP"
     gene            1135..2409
                     /locus_tag="VLF19_09130"
     CDS             1135..2409
                     /locus_tag="VLF19_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005560157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSE93455.1"
                     /translation="MLTAEENDQLTKVGPGTLIGDLMRQYWIPVVLSSELEHGGRVKR
                     VRILGEDLVVFRGPSGRVGLLGELCSHRRASLYFARNEEQGLRCVYHGWQYEPGGRCI
                     DMPNERPESCFQDKVVHPAYPCAERGGVVWTYMGPASPPPGLPDLEWAMVPEAQRFVS
                     KFWQDCNYLQALEGGVDPAHIAFLHGILDARDDGLRRALDQAAAGFGYVSQLERTPHI
                     EVADTEAGLLIGARREAPAGQYYWRITQYLLPFHTMPPPEVGSDPVFHSHVWIPIDDG
                     QLVNWCISWHPTRALTENEVAEFRAGSSIHVMDYAPATNEAYGDIRPLANRPNDYLAD
                     WEAQRTRKFFGVPGVGAQDKAITESQKTYDRSRERLGRADLGIIRVRKCLLDAAIALR
                     ARRLPPPGLDPASYRVRPASLLLPKDAPWVTR"
     gene            complement(2526..3734)
                     /locus_tag="VLF19_09135"
     CDS             complement(2526..3734)
                     /locus_tag="VLF19_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE93456.1"
                     /translation="MHDVLAALVPHRAIRSRALLACFGITGALHVATVLLQTLLPLHV
                     TALGATKTQVGLLFSVSTLVGMVLRPAVGGWVDRYGARRLIGPGALLLFATSLAFHAA
                     RTPAAVVALMAGIGVGASLVSTPASVHVAVISPPAHRGEALGIYYLFSSLPIAVAPPL
                     ALALFGAGGTGLGFAVVSAMAVVCVALALALGPRAVSAGPGEAARPRLLSRHALGLAG
                     VLVLTTCGHSAVYGFVPLSAIERGQGHLLSWFFGVYAAWIIVCRALLRGLSDRVGRVQ
                     VLVPAIGIICVGYALLALPPTGPSLIAAAFCLASGGSLQYPTLIALVADRTPPDERGL
                     AIGTISGAWDVGIVIGSAVLGLVVERAGYGPGFMVGAATAAAGLLVFVVTERRRATPS
                     SVPPSTWTRS"
     gene            complement(3761..5344)
                     /locus_tag="VLF19_09140"
     CDS             complement(3761..5344)
                     /locus_tag="VLF19_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSE93457.1"
                     /translation="MKTPVAIAGAGPVGLALACALGHHGVGCVVLEEDETLSRHSKAP
                     GVLPRTLEIFRAWGVLDRFLDTGTLLTRPGVWSPGAREPIVTIDLSQLDEMTAVPGVL
                     IIPQNRTEALLRDHALASGLADVRFGHRVAGFEQGDAGVSVAVEPATGAPYRVECEML
                     VGCDGPHSLVREALGWRLEGKTYPTRLVLADVRLGDDRNGVPWPRVAADRDGFHAALR
                     LEPDLWRIIGNVAAGVTDDAATTQDHVARRAELLFGPGPFELLWADVFRIHCRTSPGF
                     RRGRVLLAGDAAHINSPAGGQGMNSGIQDAHNLGWKLARALGGGAREPLLASYEEERR
                     PVIVANVERYTDLLTRGFLLAPPLLRRGTLAAARLAISQPPLLRRLLRRAAMLDARYP
                     RSSLISGRGALLGARAEDGPVTLPDGRHTRLLDLASREAALLLFQDLGLPAWNAAEIE
                     GIVSGVPGLRVWRLVSSARAPAGPADVVDSTGSLWRRWRPTPGSAALIRPDGHVGWMA
                     ERPNAEALSAGVRRALGQP"
     gene            5690..>6348
                     /locus_tag="VLF19_09145"
     CDS             5690..>6348
                     /locus_tag="VLF19_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSE93458.1"
                     /translation="MKAAVFHGPHKELTIEDVDIAKPIGREALVRTVASGVCHSDLHF
                     IEGLYPFAAPAILGHEAAGIVEAVGPHVTEFKPGDHVIACLSVFCGHCEYCLTGRTHL
                     CQSRPVRAANEPPKLSWKGAPVNQFANLSAYAEQMLVHESGLVKVRDDMPLDRGALIG
                     CGVTTGVGAVLNTARVEAGTTVAVYGAGGVGLAAIQGAVIAGARMIIAVDVVAHKLAT
                     AR"
BASE COUNT          951 a         2226 c         2331 g          840 t
ORIGIN      
        1 tggggttctc cacgtagtcg aagcagaaca gcagcagcgt gccgaagccg cccacctcct
       61 cgtagatcgc gtggagcttc tcggccaccg tggccggaga gcccaccagc cagttgtgcc
      121 gcgcgcagta ctcgggcgtg acgtcggaat cgggcacgtc cggcgagtgc ttcaggaact
      181 cggtgaactg gaacgacgcg aggagcggca ggaagtactc cttcatcatg cggcccatca
      241 tgccgccagc ggagaggcgc atggcctcgg cgtcggtgtc ggcgacgaag atctcgcgca
      301 cgaggcgcca ctccgcgcgt ctgggcgtcc ggccgctgcg cgccgcgccg atctcgacgg
      361 attcccagtg gctgccgacg taggtggggt tcaggttcag gctcatcggc cagaagccgc
      421 gctcgccggc gagcttcagc gtgtcggagc ccttgctcac gccggccaca ccgatcggcg
      481 ggtggggctt ctggaagggc ttgatgtgcg gcttgagcgt gtcgagcatg gtgccggtct
      541 tggtgacgtg ccagtacttg cccttgtgat cgaactcggg ctcgtcgctc cagaggcgca
      601 ggatgatgtc gagggcctcg cgcgtcatct cgcggtgctg gcccgcgttg ccgtccacgt
      661 tgaacatggc ccagtcgctg ggcagcccgc tcgccgccac accgaagttg agccggccct
      721 gcgcgaggtg gtcgagcatc gccacgcgat tggccagctc ggcggggtgg tggtagggca
      781 tgaggaatcc gccggggccg atgcgcatgc gctcggtctg caagagcgcc tgggccacga
      841 gcaggtcggg cgacggatgc ggctcccagg gcgccgtgtg gtgctcgccg atccacgcct
      901 cgctgaagcc cagctcgtcg gcccagcgga gcgtctggag gtcccactgg tgaccgtcgt
      961 acaggctgcg ctcgggcggg tgtgagggca tcgtgaagag tccgtagtcc atgccggtct
     1021 ccttgatggg agtgcggctc ccggtgcgcg gattctacga cggtttcgca ggcgcggctc
     1081 ccccgttacc atagcgccga gggagacgga caagcgccga cggaggtgcc ggctatgctg
     1141 accgcagaag agaacgacca gctgacgaag gtggggccgg ggacgctcat cggcgatctg
     1201 atgcggcagt actggatccc cgtggtgctg tcctccgagc tggagcatgg cggccgggtc
     1261 aagcgcgtca ggatcctggg cgaggacctc gtcgtcttcc gcggcccgag cgggcgcgtg
     1321 ggcctgctcg gggagctctg ctcgcaccgg cgcgcctcgc tctacttcgc gcgcaacgag
     1381 gagcaggggc tgcgctgcgt ctatcacggc tggcagtacg agccgggtgg gcgctgcatc
     1441 gacatgccca acgagcggcc ggagagctgc ttccaggaca aggtcgtcca tcccgcctat
     1501 ccgtgcgccg agcggggcgg cgtcgtgtgg acctacatgg ggccggccag cccgccgccc
     1561 gggctgccgg atctcgagtg ggcgatggtg ccggaggcgc agcggttcgt ctccaagttc
     1621 tggcaggact gcaactacct gcaggcgctc gagggcggcg tcgaccccgc gcacatcgcg
     1681 ttcctgcacg gcatcctcga cgcgcgcgac gacgggctgc ggcgcgcgct cgatcaggcc
     1741 gccgccggct tcggctacgt gtcgcagctg gagcggacgc cgcacatcga ggtcgccgac
     1801 acggaggccg ggctgctcat cggcgcgcgg cgcgaggcgc cggcgggcca gtactactgg
     1861 cgcatcacgc agtacctgct gccgttccac accatgccgc cgccggaggt gggcagcgat
     1921 cccgtcttcc actcgcacgt gtggatcccg atcgacgacg gccagctcgt gaactggtgc
     1981 atctcgtggc atcccacgcg cgcgctgacc gagaacgagg tggccgagtt ccgggccggc
     2041 tcgagcatcc acgtcatgga ctacgcgccg gcgaccaacg aggcctacgg cgacatccgg
     2101 cccctggcca accgccccaa cgactacctc gcggactggg aggcccagcg cacgcgaaaa
     2161 ttcttcggcg tgccgggcgt gggcgcgcag gacaaggcca tcaccgagag ccagaagacc
     2221 tacgatcgtt cgcgcgagcg tctgggccgg gcggatctcg gcatcatccg cgtgcgcaag
     2281 tgtctgctgg acgccgccat cgcgctgcgg gcccggcggc tgccgccgcc cgggctcgat
     2341 cccgcgagct atcgcgtccg cccggcctcg ctccttctgc cgaaggacgc gccgtgggtt
     2401 actcgatgac ctcgtccgct cgtcgcagca gccgcgaggg gtcgtgagac cgatcgcctt
     2461 gacggtgatg cccatcacga acttcgtcgg ccgcgcgatg gggaaatcgc cgggtttggc
     2521 gcccttcaag atcttgtcca cgtggacggc ggcacgctgg aaggagtcgc gcgacggcgc
     2581 tcggtgacga cgaagacgag caggcccgcg gcggccgtcg ccgcgcccac catgaagccg
     2641 gggccgtagc cggcgcgctc gacgacgagc ccgaggacgg ccgagccgat gacgatgccg
     2701 acgtcccagg ctccggagat cgtcccgatg gccagcccgc gctcgtccgg aggggtccgg
     2761 tcggccacga gcgcgatcag cgtcggatac tgcagcgagc cgccacttgc caggcagaag
     2821 gcggccgcga tgagcgatgg tcccgtcggg ggcagcgcga gcaacgcgta gcccacgcag
     2881 atgattccga tggccggcac gagcacctga acccggccga cgcggtcgga gaggccccgg
     2941 agcagcgcgc ggcagacgat gatccaggcc gcgtagacac cgaagaacca cgacagcaga
     3001 tggccctggc cgcgctcgat ggcggagagc ggcacgaatc catagacggc gctgtggccg
     3061 catgtcgtga ggacgagcac gccggccagg ccgagcgcgt gacggctgag cagccggggg
     3121 cgggcggctt cgccgggccc cgcgctcacc gcccgcggcc cgagagccag ggcgagcgcc
     3181 acgcagacga cggccatggc gctcaccacg gcgaagccga ggccggtgcc tcccgcaccg
     3241 aagagggcca gcgcgagcgg tggcgcgacc gcgatcggca gcgacgagaa gagatagtag
     3301 atgcccagcg cctcgccccg gtgcgccggc gggctgatca cggccacgtg cacggaggcg
     3361 ggggtcgaga cgaggctggc gcccacgccg atgcccgcca tgagcgcgac gacggcggcg
     3421 ggcgtgcggg cggcatggaa cgcgagcgac gtcgcgaaaa gcagcagggc gcccggaccg
     3481 atcagccgcc tcgccccgta acgatcgacc cagccgccga cggcaggccg caggaccatc
     3541 ccgacgagcg tggacacgga gaacaggagc ccgacctggg tcttcgtcgc gcccagcgcg
     3601 gtcacgtgca gcgggagcag tgtttgcagc agcacggtcg cgacgtgcag cgcgccggtg
     3661 atgccgaagc aggcgaggag cgcgcggctc cggatcgcgc ggtgcgggac cagcgccgcc
     3721 aggacgtcgt gcatgctcgc gggattctat cgctgcggca ctacggctgc ccgagcgccc
     3781 gtcgcacgcc cgcgctcagc gcctcggcgt tgggccgctc ggccatccat ccgacgtggc
     3841 cgtcggggcg gatcaacgcc gcggatcccg gcgtcggccg ccaccggcgc cacaggctgc
     3901 cggtgctgtc caccacgtcg gccggccccg ccggcgcccg ggcgctcgac accagccgcc
     3961 acacgcgcag gccgggaacc ccgctcacga tcccctcgat ctccgcggcg ttccacgccg
     4021 gcaggccgag gtcctggaac agcagcaggg ccgcctcccg gctcgcgagg tcgagcagcc
     4081 gcgtgtggcg tccatccggc agcgtcaccg gcccgtcctc ggcgcgggca ccgagcaagg
     4141 ccccgcggcc ggagatgagc gacgatcgcg gatagcgggc gtcgagcatg gccgcgcggc
     4201 gaagcagccg gcgcaggagc ggcggctggc tgatcgccag gcgcgccgcc gccagcgtgc
     4261 cgcgccggag caggggcggc gccagcagga acccgcgcgt cagcaagtcc gtgtaccgct
     4321 cgacgttggc gacgatgacg gggcgccgct cctcctcgta cgaggccagg agcggctcgc
     4381 gcgcgccgcc gccgagcgcc cgcgcgagct tccacccgag gttgtgggcg tcctggatgc
     4441 cgctgttcat gccctgaccg ccggccgggc tgttgatgtg cgcggcgtcg ccggcgagca
     4501 gcacgcggcc gcggcggaac cccgggctgg tccggcagtg gatccggaac acgtcggccc
     4561 agagcagctc gaacggtccc ggcccgaaca gcagctcggc ccggcgcgcc acgtggtcct
     4621 gggtggtcgc ggcgtcgtcg gtgactcccg cggcgacatt gccgatgatc cgccagagat
     4681 ccggctccag gcggagcgcg gcgtggaacc cgtcccgatc cgcagcgacg cgcggccacg
     4741 gcacgccgtt gcggtcgtcg ccgagtcgca cgtcggccag cacgaggcgc gtcgggtagg
     4801 tcttgccttc gaggcgccac ccgagcgctt cccgcaccag ggagtgcggg ccatcgcagc
     4861 cgacgagcat ctcgcactcg acgcgatagg gcgcccccgt cgccggctcg acggccacgg
     4921 agacgccggc gtcgccctgc tcgaatcccg cgacccgatg gccgaagcgc acgtcggcca
     4981 gaccgctcgc gagggcgtgg tcgcgcagca gtgcctcggt ccggttctgg ggaatgatga
     5041 gcaccccggg cacggcggtc atctcgtcga gctggctgag gtcgatcgtc acgatcggct
     5101 ctcgcgcgcc ggggctccag acgccggggc gagtcagcag cgtgccggtg tcgaggaacc
     5161 ggtcgagcac gccccaggcg cggaagatct ccagcgtccg cggcaggacg ccgggcgcct
     5221 tggagtggcg gctgagcgtt tcgtcctcct cgagaacgac gcagcccact ccgtgatggc
     5281 cgagagcgca ggccagcgcc agcccgacgg gcccggcccc ggcgatggcg acgggagtct
     5341 tcaccacctc cgattacacc atcctcgtca cctccctctg aaggcgtgag gagcgcgtct
     5401 gacccagtgc gcggcacact cgcgcgaggg tggctcgtgc cagcaagtgt cgcgcgctgt
     5461 catcaggtga ccacgctcga ccgcgccaag ctcacggaac gcgtcggtgt tctctcgggc
     5521 accgttctgg cgacagtcga cgggggaccg agggcggccc tcgacctcga ctgacccgac
     5581 gggcgcccga ccgacgcgca tgagcgtgac agtgcgtcga tgcgatacac ttcccgggcc
     5641 tggtccaaac gacggcgttc tcgcgaccga ctcaccaaag gagtgcgaca tgaaggctgc
     5701 ggtgttccac ggtccgcaca aggagctcac gatcgaggat gtggacatcg cgaagcccat
     5761 cgggcgcgag gcgctcgtgc gcacggtggc cagcggggtc tgccacagcg acctgcactt
     5821 catcgagggg ctctacccgt tcgcggcgcc ggccatcctc gggcacgagg cggcgggcat
     5881 cgtcgaggcg gtcggcccgc acgtgacgga gttcaagccg ggcgaccacg tcatcgcgtg
     5941 cctttcggtc ttctgcggcc actgcgagta ctgcctgacc ggccgcacgc acctctgcca
     6001 gagccggccc gtgcgggcgg ccaacgagcc gccgaagctg tcgtggaagg gcgcgccggt
     6061 caaccagttc gccaacctct cggcctacgc ggagcagatg ctggtgcacg agagcgggct
     6121 cgtgaaggtg cgcgacgaca tgccgctcga ccgcggcgcg ctcatcggct gcggcgtgac
     6181 gaccggcgtc ggcgccgtcc tcaacacggc gcgggtcgag gcaggcacga cggtggcggt
     6241 gtacggcgcg ggcggggtgg ggctggcggc catccagggc gcggtcatcg cgggcgcccg
     6301 catgatcatc gcggtagacg tcgtcgcgca caagctggcc accgcgcg
//
LOCUS       DATIAZ010000255         5565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_199507, whole genome shotgun sequence.
ACCESSION   DATIAZ010000255 DATIAZ010000000
VERSION     DATIAZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199507"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VLF19_09150"
     CDS             complement(<1..878)
                     /locus_tag="VLF19_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93459.1"
                     /translation="MKAPLPRAAVFGLAAIVVPAAIVAVLGYLSLRQWESSAELLFRE
                     QARDMATMAVDKIHMILRRAEDDALARLEAALAAEQAREAALQRFLADTPIVHSLYLF
                     DRAGRLLYPASPGGDAAVFAALLAEVSPGLWERGGRRHVAAGGRPAVAAVLRSASGPL
                     LLAALTASVETLRREVFETTIRPLEASTICAVIDEHGRPIYAQRPVDQAQLIVAVPFR
                     EGLPDWRLALYEPPGTSPRESVRRQVVVFTAAFGVMLAVIVAGVVATWRLVRRETEMA
                     RLKSDFVANVSHDLKTP"
     gene            complement(885..1196)
                     /locus_tag="VLF19_09155"
     CDS             complement(885..1196)
                     /locus_tag="VLF19_09155"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSE93460.1"
                     /translation="MIPQLRAWHEKYQTAGLTIVGVHSPEFPWERPTESVVAATKKLG
                     ILYPVVQDNDFAIWKRFGVRAWPTQVLVDRRGVVRFRHIGEGAYEETEAMIQRLLAES
                     G"
     gene            complement(1212..1409)
                     /locus_tag="VLF19_09160"
     CDS             complement(1212..1409)
                     /locus_tag="VLF19_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93461.1"
                     /translation="MRRAAMAVLAALALVAGAAGLAPGQALRVGAPAPDVAGAPWINS
                     ASLTIQELRGRVVLVEFWTYG"
     gene            1732..3363
                     /gene="pgi"
                     /locus_tag="VLF19_09165"
     CDS             1732..3363
                     /gene="pgi"
                     /locus_tag="VLF19_09165"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSE93462.1"
                     /translation="MARAWLTELPEWKDLTAHYERVRDIHLRRLFADDPRRGERLTAE
                     GAGLFLDYSKNRVTDETLSLLGRLAQAARLTERREAMFRGEKINATERRSVLHVALRM
                     PRDQRLVVDGADVVAPVHAVLDKMADFAERVRSGRWRGHTDRPIRTVVNIGIGGSDLG
                     PAMATDALRVFTAPNLAYRFVSNVDGADLTAATRGCDPAETLFIVASKTFTTLETMTN
                     ARAARAWCLAGLGDERAVARHFVAVSTNIPEVTKFGIDAANAFEFWDWVGGRYSMDSA
                     IGLSLMIAIGPQNFRDMLGGFHAMDEHFRTAPLERNLPVILGLLGVWYIDFFGAQSHA
                     VLPYSHDLGRLSAYLQQLDMESNGKHVSLEGRTVDYDTGPIVWGTPGTNGQHAYYQLL
                     HQGTRLVPADLIGFCEKLSPIADQHDLLMANLFAQAEAFAFGKTEAEVKAEGVPEHQV
                     THRTFEGNRPTNMILADRLSPATLGALIALYEHKIFVQGAIWDINSFDQWGVELGKVL
                     AARIVPELAADTALAHDSSTNALIRRYRARRRAGG"
     gene            complement(3445..4137)
                     /locus_tag="VLF19_09170"
     CDS             complement(3445..4137)
                     /locus_tag="VLF19_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HSE93463.1"
                     /translation="MSPAIERLDDIAMDEVRLHTPVAPEDIARLKIGDIVFLDGFVYT
                     GREGVYRKVVDEGRGLPESVRRLTNVNFHCSPAASVRPDGSYAVEAVTATASFRFGKS
                     MARWFERSGSKVIVGKAGLTELAYREWFVPHGAIYLTTVGYGLGAAYGKCIRGVREVH
                     WLEALGLAQALWVLEVERLGPFLVEGDAQGRSLFALANREIDRRLDETYAGLPPYSMS
                     RFGESTPKQEIV"
     gene            complement(4134..5072)
                     /locus_tag="VLF19_09175"
     CDS             complement(4134..5072)
                     /locus_tag="VLF19_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HSE93464.1"
                     /translation="MAGAIPAKLIEDTARALTARAAIDIPRDFREGVRRARDAETGRL
                     PRFVLDEMLANWELATADRRPMCADTGLPRYYVRVGNEAALEGGFVALERALRRATAE
                     ATAAIPLRPNRVHPLTRADHNNNVGIHAPEVTYAFEPGRDFIDLTTVHKGGLFGTDYR
                     MLFPADGVGGLKRFFVDTLVQFGRRGLACQPAIIGVGIGGAKDTCVRLGKEAACLRLV
                     GSRNPDLTIAALEDELTRLGNHIGIGAMGFAGTSLVVATHVEVAYSHTGGMPVGIHAF
                     CLSSRRATARIHPDGRVELRDDPGWFTDYYRREGTE"
     gene            complement(5085..5288)
                     /locus_tag="VLF19_09180"
     CDS             complement(5085..5288)
                     /locus_tag="VLF19_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93465.1"
                     /translation="MTDELAGLDATAQAQLVRRNPLGPAAGDIISARSAVNSDRAAGI
                     DRAHRSLGLSRSDASFDGVAAVG"
     gene            complement(5300..>5565)
                     /locus_tag="VLF19_09185"
     CDS             complement(5300..>5565)
                     /locus_tag="VLF19_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE93466.1"
                     /translation="GDSPVLSTAFTEAVSPAYLGAALALRSLLGFGAGAIAPLVFGAV
                     LDWTNPPGVAPTAWGWAFVVLGLGGLGATMCAWGVTLRRPAER"
BASE COUNT          827 a         2048 c         1934 g          756 t
ORIGIN      
        1 ggcgtcttca ggtcgtggga gacgttggcg acgaaatccg acttgagccg cgccatctcc
       61 gtctcccggc gcaccaggcg ccaggtggcg acgacgccgg ccacgatgac ggcgagcatc
      121 acgccgaagg ccgcggtgaa gaccacgacc tggcggcgca ccgactcgcg cggcgaggtc
      181 cccggcggct cgtagagggc caggcgccag tccgggagcc cctcgcggaa gggcacggcg
      241 acgatgagct gggcctggtc gacgggccgc tgcgcgtaga tgggccggcc gtgctcgtcg
      301 atcaccgcgc agatcgtcga cgcctcgagc ggcctgatgg tggtctcgaa gacctcgcgg
      361 cgcagggtct ccacgctcgc cgtgagcgcg gcgagcaaga gcgggcccga ggcggaccgc
      421 agcactgccg cgaccgcggg gcgccccccg gccgcgacgt ggcggcgccc accccgctcc
      481 cacagccccg gcgagacctc cgcgagcaag gccgcgaaca ccgcggcgtc gccgcccggg
      541 gacgccggat acagcagccg gcccgcccgg tcgaagaggt acaggctgtg gacgatcggg
      601 gtgtcggcca ggaagcgctg cagcgccgcc tcgcgggcct gctccgcggc cagggcagcc
      661 tcgagccggg cgagggcgtc gtcctccgcc cggcggagaa tcatgtggat cttgtccacg
      721 gccatcgtcg ccatgtcgcg cgcctgttcg cggaacagca gctccgcgga cgactcccac
      781 tggcgcagcg acaggtagcc gagcacggcc acgatggccg ccggcacgac gatggcggcc
      841 agcccgaaga ccgccgcgcg cgggagcggc gccttcacag cggctcagcc actctccgcg
      901 aggagccgct ggatcatcgc ctcggtctcc tcgtaggcgc cctcgccgat gtgccggaac
      961 cggacgacgc cgcggcgatc gacgagcacc tgtgtcggcc aggcgcggac gccgaagcgc
     1021 ttccagatgg cgaagtcgtt gtcctgcacg acggggtaga gaatacccaa cttcttcgtt
     1081 gcggccacga cgctctccgt cggcctttcc caggggaact ccggcgagtg caccccgacg
     1141 atggtgagcc ccgctgtctg gtacttctcg tgccaggccc gcaactgcgg gatcacgttc
     1201 cggcagttga ttcagccgta cgtccagaac tccacgagca cgacgcgacc gcgcagctcc
     1261 tggatggtca gcgacgcgct gttgatccac ggcgcgcccg cgacgtccgg cgccggcgcg
     1321 ccgacgcgca gcgcctggcc cggggccaga cccgccgccc cggccacgag cgccagcgcg
     1381 gcgagcacgg ccatcgccgc gcgtctcacg tcatctccat cggcacgtca tcgagtccca
     1441 ccatctcgcg cctcctcgtg cccgattctc gccatcgtgg caggacggcg atcggaggtt
     1501 gtcatcgtgg tgtgaaatcc accgatgttc ccgttcatcg ccttgcgccc tgcctctgat
     1561 cggcctaccg ggtggagtgt tcgaccgccc ggcctcccct gcccgggcca ccccgaccgc
     1621 gggaggacgc cggccacccc acggcggcct tgccgcccac agccggtgtg gtgcattatg
     1681 gcgctcagtc cggggagaac ggcgcgggat cccgaaacgg agggcaccaa cgtggcgaga
     1741 gcatggctga ccgaactgcc cgagtggaaa gacctcaccg ctcactacga gcgggtccgg
     1801 gatatccatc tccgcaggct cttcgctgac gatccgcgtc gcggcgagcg gctcactgcg
     1861 gagggtgccg gcctcttcct ggactattcc aagaaccgcg tcaccgacga gacactgtcg
     1921 ctcctgggcc gtctcgccca ggcggctcgg ctgaccgagc gccgcgaggc gatgttccgc
     1981 ggcgagaaga tcaacgcgac cgagcgccgg tcggtcctgc acgttgccct ccgcatgccg
     2041 cgcgatcagc ggctggtcgt ggacggcgcc gacgtggtcg ccccggtgca cgcggtgctc
     2101 gacaagatgg cggatttcgc cgagcgcgtg cgcagcgggc ggtggcgcgg ccacaccgac
     2161 cgtccgatcc gcaccgtggt caacatcggc atcgggggct cggatctcgg ccccgccatg
     2221 gccaccgacg cgctcagggt cttcaccgcc ccgaacctcg cctaccgctt cgtctcgaat
     2281 gtggacggcg ccgatctcac ggccgccacc cgcggctgcg atcccgccga gacgctcttc
     2341 atcgtggcct ccaagacctt cacgacgctg gagacgatga ccaacgcccg cgccgcccgg
     2401 gcctggtgcc tcgcagggct cggcgacgag cgcgctgtcg cccgccactt cgtggccgtc
     2461 tccaccaaca tccccgaggt cacgaagttc ggcatcgacg ccgccaacgc cttcgagttc
     2521 tgggactggg tgggcgggcg ctactcgatg gactcggcca tcgggctctc gctcatgatc
     2581 gccatcggcc cccagaactt ccgggacatg ctcggcggct tccacgccat ggacgagcac
     2641 ttccgcaccg cgccgctcga gcgcaacctg cccgtcatcc tcggcctgct cggcgtctgg
     2701 tacatcgatt tcttcggcgc ccagagccac gccgtgctgc cgtacagcca cgacctcggc
     2761 cgcctgtcgg cctacttgca gcagctcgac atggagtcga acggcaagca cgtgagcctg
     2821 gagggccgga ccgtcgacta cgacaccggg cccatcgtgt ggggcacgcc cgggaccaac
     2881 gggcagcatg cctactacca gctcctgcac cagggcaccc ggctggtgcc ggccgatctc
     2941 atcggcttct gcgagaagct atcgccgatc gccgaccagc acgacctgct gatggcgaac
     3001 ctcttcgccc aggccgaggc cttcgccttc ggcaagacgg aagccgaggt gaaggcggag
     3061 ggagtgccgg agcaccaggt cacgcaccgg accttcgagg gcaaccggcc gacgaacatg
     3121 atcctggccg accggctgag cccggccacg ctgggcgccc tgatcgccct ctacgagcac
     3181 aagatcttcg tgcagggagc catctgggac atcaactcgt tcgaccagtg gggcgtggag
     3241 ctgggcaagg tgctcgccgc gcggatcgta ccggagctgg ccgcggacac ggctctcgcg
     3301 cacgacagct cgaccaacgc gctgatcagg cgctaccgcg ctcgccgccg cgccgggggg
     3361 tagcgccgag tctccttcct cggacatctc gctggcatcc gcggacaact cgacgggccg
     3421 tcgtcgtaac gagacgcggt ccggctacac gatctcctgc ttgggcgtgc tctcgccgaa
     3481 gcggctcatc gagtagggcg gcaggcccgc gtaggtctcg tcgaggcgcc ggtcgatctc
     3541 ccggttggcg agcgcgaaga ggctccggcc ctgggcgtcg ccctcgacga ggaacggccc
     3601 gagccgctcg acctccagca cccagagcgc ctgggcaagc cccagcgcct cgagccagtg
     3661 gacctcgcgc acgcccctga tgcacttgcc gtaggcggcg ccgagcccgt agcccaccgt
     3721 ggtgaggtag atggcgccgt ggggcacgaa ccactcacgg taggccagct cggtgagccc
     3781 cgccttgccc acgattacct tcgagcccga gcgctcgaac cagcgcgcca tcgacttgcc
     3841 gaagcggaac gacgcggtgg ccgtcacggc ctccacggcg tacgagccgt ccggccgcac
     3901 gctcgccgcc ggcgagcagt ggaagttcac gttggtcagg cgccgcacgc tctcgggcag
     3961 cccgcgtccc tcgtccacga ccttgcggta gacgccctcg cggcccgtgt agacgaagcc
     4021 gtcgaggaag acgatgtcgc cgatcttcag gcgcgcgatg tcttccggcg ccaccggcgt
     4081 gtggagccgc acctcgtcca tcgcgatgtc gtcgaggcgc tcgatggccg ggctcactcg
     4141 gtcccctcgc ggcgatagta gtcggtgaac cacccggggt cgtcgcggag ctcgacgcgg
     4201 ccatcgggat ggatgcgggc cgtcgcccgc cgcgacgaca gacagaacgc gtggatgccg
     4261 accggcatgc cccccgtgtg cgagtaggca acctcgacat gcgtggccac gaccagcgag
     4321 gtccccgcga agcccatcgc gccgatgccg atgtgattgc cgagacgcgt cagctcgtcc
     4381 tcgagggccg cgatcgtgag gtcggggttg cggctgccca ccaggcgcag gcacgcggcc
     4441 tccttgccga gccgcacgca ggtgtccttg gcgccgccga tgccgacgcc gatgatggcg
     4501 ggctggcagg cgagcccacg ccgcccgaac tgcacgagcg tgtccacgaa gaagcgcttg
     4561 agcccgccga cgccgtcggc gggaaacagc atgcggtagt cggtgccgaa gaggcccccc
     4621 ttgtgcacgg tggtgaggtc gatgaagtcg cggccgggct cgaaggcgta ggtgacctcg
     4681 ggggcgtgga tgccgacgtt gttgttgtgg tcggcgcgcg tgagcgggtg cacgcggttc
     4741 ggccgcagcg ggatggcggc cgtggcctcg gcggtggccc ggcggagcgc gcgctcgagg
     4801 gcgacgaagc cgccctccag ggccgcctcg ttgccgacgc gcacgtagta gcgcggcagc
     4861 ccggtatctg cgcacatggg ccggcggtcg gccgtggcca gctcccagtt cgccagcatc
     4921 tcgtcgagga cgaagcgcgg cagccgtccg gtctccgcgt cgcgggcgcg ccgcacaccc
     4981 tcgcggaagt cgcgcgggat gtcgatggcc gcgcgcgccg tcagcgcgcg cgcggtgtcc
     5041 tcgatcagct tcgcggggat cgcgccggcc atcgtgggcc caccctagcc gacggccgcg
     5101 accccgtcaa acgacgcatc cgagcgcgac aggccgagcg atcggtgcgc gcgatcgatc
     5161 cccgccgcgc ggtcggagtt caccgcgctt cgcgcgctga tgatgtcacc ggccgcggga
     5221 ccgagggggt tgcggcgcac cagctgcgcc tgcgcggtgg cgtcgaggcc cgcgagctcg
     5281 tcggtcacgc tcatcgcggt cacctctccg cgggacgccg cagggtgacg ccccacgcgc
     5341 acatcgtcgc gccgagcccg cccagcccga gcacgacgaa ggcccagccc caggcggtgg
     5401 gcgccacgcc gggcgggttc gtccagtcga gcaccgcgcc gaacaccagc ggggcgatgg
     5461 cgccggcgcc gaagccgagc agcgagcgca gcgcgagcgc cgcgccgagg tacgccgggc
     5521 tcacggcctc ggtgaacgcg gtcgagagca cgggcgagtc gccca
//
LOCUS       DATIAZ010000256         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_463921, whole genome shotgun sequence.
ACCESSION   DATIAZ010000256 DATIAZ010000000
VERSION     DATIAZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463921"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..555)
                     /locus_tag="VLF19_09190"
     CDS             complement(115..555)
                     /locus_tag="VLF19_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSE93467.1"
                     /translation="MKVLVAKPGLDGHDRGAKVVAQALRDAGVEVIYTGLKRTPEEIV
                     QEAIQEDVDAVGLSILSGAHLTLSRRVVEGLRGLGASDVHVVVGGTIPPRDVEALLRL
                     GVRRVFPMGTPLPEIVKAFAALGAERPERGEPSRSPCDHARDQT"
     gene            complement(552..1058)
                     /locus_tag="VLF19_09195"
     CDS             complement(552..1058)
                     /locus_tag="VLF19_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSE93468.1"
                     /translation="MASGVRSGVTTVGVISDTHGVLRPEAVEALRGVDRIVHAGDIGA
                     LEVLERLAGVAPVTAVRGNNDGDPWARGIPETEALEVGGLWLYVLHDRATLDLDPRAS
                     GFGAVISGHSHRPGQEERDGVLYFNPGSAGPRRFRLPVAVGRLTIAAGHVTGRIVPLA
                     VGRPLGAS"
     gene            complement(1031..1459)
                     /locus_tag="VLF19_09200"
     CDS             complement(1031..1459)
                     /locus_tag="VLF19_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSE93469.1"
                     /translation="MTYFRAVDPFPLESADHTKLHEFYSRLAGGRLCTTRCAGCGVTA
                     WPPRGFCAECTSDRFEWVELAREGVIHGFSVQETGLPAGFDAPRVFAVVKLGSHRIFT
                     VVRDADPATLAIGQRVRLAPLRVSDDSRGIPRWLPAFVPA"
     gene            complement(1456..2421)
                     /locus_tag="VLF19_09205"
     CDS             complement(1456..2421)
                     /locus_tag="VLF19_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSE93470.1"
                     /translation="MSAAIERVAVLGAGRMGRQIALACALGGCQVLLADLKSRGAAAT
                     AVVLADARREIARDLGLMAEEGVIAPAAVAPALARIQDRAGLGGLDACGFIQEALPES
                     RDLKREVFASLTGRLAPDAIVASTSSTISPAHLADAVPGSERFLVAHWLNPAHIIPLV
                     EVVPGEKTATDVVARAVAFLEALGKVPVECGDAAGFIVPRLQILLMNEAVRLVEEGVA
                     TPEQVDRAIRAGLGFRYATVGVFEFIDWGGVDILARASRFMVQATGDERYRAPRLVEE
                     KMARNELGPKTGRGFFEYSGERRDAFEAGKLRALLRQLKRAREAR"
     gene            complement(2418..3587)
                     /locus_tag="VLF19_09210"
     CDS             complement(2418..3587)
                     /locus_tag="VLF19_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007260856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HSE93471.1"
                     /translation="MRKVALVGAGVSRFGVRKASYRDLIWEAGKACFDSVPGLRPRDV
                     EGLVVGSVMPERTAFQSHVSSLAAEALGIKPSALSARTEHMCASGTVGIRYAYAFIAA
                     GLADVVVVLGVEKLNQPDGAEAILNMGTGVDREWEAAFGLTAPPCFALAAQRHMAEYG
                     TTEEQLALVGVKNHTHAARNPNAHFTKGATLDQVLCSRMIANPLRLFMCSPITDGAAA
                     VVVAGEERARELTDRPVWIRGTGQALDGFQLTSLHEDYARWPALERAAESAYRMAGIG
                     ARDVDVAEVHDCFAIAELIAYEELGFCEKGEAGPFAAAGRSDYGGDVVVNPRGGLIGC
                     GHPLGATGVAQAAEIFAQLRGEAGARQVDGAAIGLTHNNSGMGEHVVMIYGRETS"
BASE COUNT          526 a         1363 c         1266 g          472 t
ORIGIN      
        1 gcaccatgag cacggggtcg ccatggcctt cgtccacgta gtgcatgcgc gcgccgtcca
       61 ccacgagcca gcgtgactcg aacggatact ccgcgcggtc aatccatgcg ggcgtcacgt
      121 ctgatcccgc gcatggtcgc acggcgaccg ggaaggctcg ccccgttccg gccgctcggc
      181 tcccaaggcc gcaaaggcct tcacgatctc cgggagcggc gtgcccatcg ggaacacccg
      241 ccgcaccccc aggcgcagca acgcctcgac gtccctggga ggaatcgtgc cgcccacgac
      301 gacgtggacg tcggatgcac ccagcccccg cagcccctcc acgacgcggc gggagagcgt
      361 caggtgcgcc cccgagagga tggacagccc cacggcgtcc acgtcctcct ggatcgcctc
      421 ctgcacgatc tcctcgggcg tccgcttgag ccccgtgtag atcacctcga cgccggcgtc
      481 ccgcagcgcc tgtgcgacca ccttcgcgcc gcggtcgtgg ccgtcgagcc cgggcttggc
      541 gacgaggacc ttcatgacgc gccgagcggg cgcccgacgg ccagcggcac gatccgtccg
      601 gtcacgtggc cggcggcgat ggtgaggcgg ccgacggcga cgggcaggcg gaagcgccgc
      661 ggaccggcgc tacccggatt gaagtagagg acgccgtccc gctcctcctg gccgggccga
      721 tgcgagtgac cgctgatgac cgcgccgaag ccggacgccc ggggatccag gtcgagcgtg
      781 gcacggtcgt ggagcacgta gagccacagg ccgccgacct cgagcgcctc ggtctcgggg
      841 atgccgcgcg cccatgggtc accgtcgttg ttgccccgca cggccgtgac cggcgccacg
      901 ccggccaggc gctcgagcac ctcgagcgcg ccgatgtcgc ccgcgtgaac gatgcggtcc
      961 accccgcgca gcgcttccac ggcctcgggg cgcagcacgc cgtgcgtgtc cgagatgact
     1021 ccgacagtgg tcacgccgga acgaacgccg gaagccatcg ggggataccc ctggagtcgt
     1081 cgctcacgcg cagcggtgcg agccgcaccc gctggccgat cgccagcgtc gccggatcgg
     1141 cgtcgcgcac gacggtgaag atccggtggc tgccgagctt gacgacggcg aacacgcggg
     1201 gagcgtcgaa gcccgccggc aggcccgttt cctggacgct gaagccgtgg atgacgccct
     1261 cccgggccag ctcgacccac tcgaagcggt cggacgtgca ctcggcgcag aagccgcgcg
     1321 gcggccacgc agtgacaccg cagccggcgc agcgcgtggt gcagagccgt cccccggcga
     1381 gccgcgaata gaactcgtgc agcttcgtgt ggtccgccga ttccagcggg aacgggtcga
     1441 cggctcggaa gtaggtcatc gcgcctcgcg cgcccgcttg agctgccgca gcaacgcgcg
     1501 gagcttgccc gcctcgaacg cgtcacgccg ctcgccgctg tactcgaaga agccgcggcc
     1561 ggtcttgggc cccagctcgt tgcgcgccat cttctcctcg acgagacgcg gcgcgcgata
     1621 gcgctcgtcg ccggtcgcct gcaccatgaa gcggctggca cgcgccagga tgtcgacacc
     1681 gccccagtcg atgaactcga agacgccgac ggtcgcgtag cggaagccga gccccgcgcg
     1741 gatcgcacgg tcgacctgct ccggcgtcgc gaccccctcc tccaccagcc gcacggcctc
     1801 gttcatgagg aggatctgga gccgaggcac gatgaacccc gccgcgtcgc cgcactcgac
     1861 cgggaccttg ccgagcgcct ccaggaacgc cacggcacgg gcgaccacgt ccgtggcggt
     1921 cttctcgccc ggaaccactt ccacgagcgg gatgatgtgt gcggggttca gccagtgggc
     1981 gacgaggaag cgctccgacc ccggaacggc gtcggccagg tgcgccggcg agatcgtcga
     2041 cgatgtcgag gcgacgatgg catcgggggc gagccggccg gtgagcgacg cgaagacctc
     2101 gcgcttgagg tcgcgcgact cgggcagcgc ctcctggatg aagccgcacg cgtcgaggcc
     2161 gccgaggccc gcacgatcct ggatgcgcgc cagggccggc gccaccgcgg ccggggcgat
     2221 cacgccctcc tcggccatca ggccgaggtc gcgcgcgatc tcgcgccgcg cgtccgccag
     2281 gactaccgcc gtcgccgcgg cgccgcggga cttgaggtcg gcgagcagca cctgacagcc
     2341 gcccagggcg caggcgagcg cgatctggcg ccccatgcgc ccggcgccga ggacggcgac
     2401 gcgctcgatc gcggcgctca cgacgtctca cgtccgtaga tcatcaccac gtgctcgccc
     2461 atgccgctgt tgttgtgggt gaggcctatc gccgcgccgt ccacctggcg cgctccggct
     2521 tctccgcgaa gctgggcgaa tatctccgcg gcctgggcga cgcccgtcgc gcccagcgga
     2581 tggccgcagc cgatgagacc accgcgcggg ttgacgacca cgtccccgcc atagtcgctg
     2641 cgccccgcgg ccgcgaacgg ccccgcctcg cctttctcgc agaagcccag ctcctcgtag
     2701 gcgatcagct ccgcgatggc gaagcagtcg tggacctccg cgacgtcaac gtcccgcgcc
     2761 ccgatgcccg ccatgcggta ggcgctctcg gccgcgcgct cgagggccgg ccaccgggcg
     2821 tagtcctcgt ggagcgaggt gagctggaag ccgtcgagcg cctggccggt gccgcggatc
     2881 cacaccgggc ggtcggtcag ctcgcgcgcg cgctcctcgc cggcgacgac gaccgcggcg
     2941 gccccatcgg tgatcggcga gcacatgaag aggcgcagcg ggttcgcgat catccgggag
     3001 cagagcacct ggtcgagcgt cgcgcccttg gtgaagtgcg cgttcgggtt ccgggcggcg
     3061 tgggtgtggt tcttcacgcc gaccagggcg agctgctcct cggtggtccc gtactccgcc
     3121 atgtgccgct gggcggcgag ggcgaagcac ggcggcgccg tcagcccgaa cgccgcctcc
     3181 cactcgcggt ccacgccggt gcccatgttc agaatggcct cggcgccgtc cggctggttc
     3241 aatttctcga cgccgaggac cacgacgacg tcggcgagtc ccgcggcgat gaatgcgtac
     3301 gcgtagcgga tgccgacggt ccccgaagcg cacatgtgct cggtccgcgc gctgagcgcg
     3361 ctcggcttga tgccgagagc ctcggcggcc agcgacgaga cgtggctctg gaacgccgtg
     3421 cgctccggca tcacggagcc gacgacgagc ccctcgacgt ccctggggcg cagccccggc
     3481 acggagtcga agcacgcctt gcccgcttcc cagatgaggt cgcggtacga ggccttccgc
     3541 accccgaacc tcgacacgcc tgcgccgacc agcgcaacct tcctcatggc gcttctccca
     3601 ggtcccggag gaccgcgccg acgcgtt
//
LOCUS       DATIAZ010000257         5233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1515053, whole genome shotgun sequence.
ACCESSION   DATIAZ010000257 DATIAZ010000000
VERSION     DATIAZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515053"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="lpxC"
                     /locus_tag="VLF19_09215"
     CDS             <1..705
                     /gene="lpxC"
                     /locus_tag="VLF19_09215"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HSE93472.1"
                     /translation="TRIHTVEHLMAAAAGLGIDNLFIDVEGPEIPAADGSAKPFVALL
                     ATAGRSEQSAPRRPIVVTKPVRVGGGGRWIQIVPSDTLRISYTLDNDHPAVGTQALTC
                     TPTERMFVEDFAPARTYGFLKDLGVMRKNGLARGASLENAIGVGKRGVLNGLRYRDEF
                     VRHKILDLIGDLALLGRPLVGHVIARNAGHALNFELVVAVQRALGLDRRPAGAAVVPL
                     EVARRPLVSVPGLATI"
     gene            899..1465
                     /locus_tag="VLF19_09220"
     CDS             899..1465
                     /locus_tag="VLF19_09220"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HSE93473.1"
                     /translation="MRGVRTILVTLMAAAALAAAAPAGAELRLSDLAVYLNDHEVTIH
                     VVALGAVPAAFHEPIQTGIPAHMRFTIELWQYNRMRRDQLLTRYVVERLLSYNVVAQE
                     YRVTFQKGETRATYATRDVRDAQRVISEVRAIKLSPPSPLDPDGVIYVRVYAETALSG
                     ENSFVARMAGTAEQTELRSEYRTLRRVQ"
     gene            1482..3704
                     /locus_tag="VLF19_09225"
     CDS             1482..3704
                     /locus_tag="VLF19_09225"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE93474.1"
                     /translation="MPLFVEHGRRRRNFLLASVFLLLLLVATALNFGMRVPDVPLASN
                     IVVFALFNLNLIVFLLLLVLLIRNLVKLWFERRQKLIGSRFKTKLVLTFLLLALTPAA
                     LIFLIASNFINKSIEGWFKPQVERPLDQAMTVAQTYYQNLQSTALRHAQQIARVIDRD
                     HLLEEDRRQALAAYLVEQREQLGLSALTVFAASGQELVHVKDPTLGVLPTRDVNEGQL
                     RAGLAGQGVTTVREMFSGDLVEAIVPIFTVTPERRVVGAVVVGTQITERLEQKIRGIE
                     QAFKEYKQLKLVKNPVKGIYILLFLFLTLVVIFSFAWFGLYLARGITGPIAELAEGTR
                     EVAAGNLGYKVQARADDEIGVLVDSFNRMTDDLAQSKRQLEEAYLDLQDKHTELEDRR
                     RYIEIVLEAVTTGVLSLDPLGRLTTLNRAAAAMFGLDAATSVGRPVEEVFGNADVRDV
                     VALVHRVRRARTGAVEQDLRLRRNGTAVSLLASATALRGPEGEYAGAVVVFDDLTELL
                     KAQRLAAWREVAQRIAHEIKNPLTPIQLSAQRLRRRLARTAGDEQLVSECTETIIQEV
                     DGLKRLVDEFSRFARMPVLTPRPTDLRGIVDTVAALYRESYPGLTLTTRYGDDLPLLD
                     VDPDHVKRAVLNLVENAVDAVAGQGLVEVEIVHRAATGRIELVVSDSGPGISVEDKER
                     VFLPYFSTKTTGMGLGLPIVYEVVAEHGGAVRVEDNRPHGSRFVVDLPVARTPAPLGA
                     "
     gene            3718..5094
                     /locus_tag="VLF19_09230"
     CDS             3718..5094
                     /locus_tag="VLF19_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE93475.1"
                     /translation="MADEHILIVDDEPAIQSTLRGVLEDEGYRVSTVGSGRQGIDLLA
                     DEAPDLVFLDVWMPGMDGLDTLVQIKTLRPETAVVMISGHATIETAVKATRLGAYDFI
                     EKPLSLDKTLLTVSRALEHARLARENAALRQRLEERLEIIGDSPVMRRLRDDITTAAP
                     SNGRVLIHGENGSGKELVARAIHALSSRRDRPFVEVNCAAIPEELIESEMFGHEKGAF
                     TGAVARRRGRFEVADGGTLFLDEIGDMSLKTQAKVLRALEEQAFERVGGRDKIKVDVR
                     VIAATNRDLAAMFGRGFREDLYYRLNVIPIEVPPLRARRDDLPLLVDHFIRVFCAENG
                     KRVKTVSGEALAYFLAYDWPGNVRELRNLVERLVIMAPGDVIGVDDVPAPVRPKEAAL
                     REAAAGGSADAGERSLREARDNFERAYILTELRANEWNMTRTAERLGIERSHLYRKIK
                     AYGITPPK"
BASE COUNT          802 a         1879 c         1774 g          778 t
ORIGIN      
        1 acccgcatcc acacggtcga gcacctcatg gctgcggcgg ccggactcgg catcgacaac
       61 ctgttcatcg acgtcgaggg gcccgagatc ccggctgcgg acggcagcgc caagccgttc
      121 gttgcgctcc tggcgacggc cggccggagc gagcagtcgg cgccgcggcg gcccatcgtc
      181 gtcaccaagc cggtccgcgt cggtggcggc ggtcgctgga tccagatcgt gccctccgac
      241 actctgcgca tcagctacac gctcgacaac gaccacccgg ccgtcggcac gcaagcgctc
      301 acgtgcacgc cgaccgagcg catgttcgtg gaggacttcg cgccggcgcg cacgtacggg
      361 ttcctcaagg acctcggcgt catgcgcaag aacggcctgg cgcgcggggc gtcgctggag
      421 aacgccatcg gcgtgggcaa gcgcggcgtg ctgaacggct tgcgctaccg cgacgagttc
      481 gtgcgccaca agatcctcga cctcatcggc gacctggcgt tgctcggccg cccactggtg
      541 ggtcacgtga tcgcccgcaa cgcggggcat gcgctgaact tcgagctggt cgtggccgtc
      601 cagcgcgcgc tcggcctgga tcggcgaccg gccggcgccg cggtggtgcc gctcgaggtc
      661 gcgcgacggc cgctggtgtc ggtgccaggt ctcgccacga tttgacgatt gaccgcatcg
      721 gagggccgag acaggccctc cgggatctcc tccgaccccc tctcacatcg gaggggacga
      781 gacctccctc cgagacctcc ccggttcacg cgagcgacgg cgccgcgcac gcgcgccagg
      841 ctgcgcccga agcgccgttc tccgatggtg cggtgtgaca taatgacggg gttggcgtat
      901 gcggggagta cgcacgatcc tggtcaccct gatggcggca gccgcgctcg ccgccgcggc
      961 gcccgcgggg gccgagctcc gcctcagcga cctcgccgtc tacctcaacg accacgaggt
     1021 gaccattcac gtcgtcgccc tcggcgccgt gccggccgcc ttccacgagc cgatccagac
     1081 cggcatcccc gcgcacatgc gcttcacgat cgagctgtgg cagtacaacc ggatgcgccg
     1141 cgaccagctc ctgacccggt acgtcgtcga gcggctgctc tcctacaacg tcgtcgccca
     1201 ggagtaccgg gtgaccttcc agaagggcga gacgcgcgcg acctacgcca cccgcgacgt
     1261 gcgcgacgcg caacgggtga tctcggaggt gcgcgcgatc aagctctcgc cgccgtcccc
     1321 gctggatccc gacggcgtca tctacgtccg cgtgtacgcc gagaccgcgc tgagcggcga
     1381 aaactccttc gtcgcgcgga tggcgggcac ggccgagcag acggagctgc ggtccgagta
     1441 ccgcacgctc cgccgggtgc agtgacggtg gggatggcct gatgccgctg ttcgtcgagc
     1501 acgggcgccg gcgccggaac ttcctgctgg ccagcgtctt cctgctgctc ctcctcgtcg
     1561 ccacggcgct gaacttcggg atgcgggtgc cggacgtccc gctcgcgtcc aacatcgtcg
     1621 tcttcgccct cttcaacctg aacctcatcg tcttcctgct gctgctcgtg ctcctcatcc
     1681 gcaacctggt caagctgtgg ttcgagcgac ggcagaagct catcggctcg cggttcaaga
     1741 ccaagctggt gctgaccttc ctcctgctcg cgctcacgcc ggccgcgctg atcttcctga
     1801 tcgcgtcgaa cttcatcaac aagtcgatcg agggctggtt caagccccag gtggagcggc
     1861 cgctcgacca ggccatgacg gtggcccaga cgtactacca gaacctccag tccaccgccc
     1921 tgcgccacgc gcagcagatc gctcgcgtga tcgaccgcga ccaccttctc gaggaggacc
     1981 ggcgtcaggc gctggccgcc tatctggtgg agcagcggga gcagctcggc ctgagcgcgc
     2041 tgacggtgtt cgcggccagc ggccaggagc tcgtgcacgt caaggacccg acactcggcg
     2101 tgctgcccac gcgcgacgtc aacgaggggc agctccgggc cggcctggcc ggtcagggcg
     2161 tgaccacggt gcgcgagatg ttctccggcg atctcgtgga ggccatcgtg cccatcttca
     2221 ccgtgacgcc ggagcgccgc gtggtcggcg ccgtggtggt cggcacccag atcaccgagc
     2281 gcctcgagca gaagattcgg ggcatcgagc aggccttcaa ggagtacaag cagctcaagc
     2341 tcgtgaaaaa ccccgtgaag ggcatctaca tcctgctctt cctctttctg accctggtcg
     2401 tcatcttctc gttcgcctgg ttcggcctgt acctggcccg cggcatcacc ggccccatcg
     2461 ccgagctggc cgaggggacg cgggaggtgg ccgccggcaa cctcggctac aaggtccagg
     2521 cgcgcgccga cgacgagatc ggcgtgctgg tggactcgtt caaccgcatg accgacgacc
     2581 tcgcgcagtc caagcggcag ctcgaggagg cctatctcga cctccaggac aagcacaccg
     2641 agctggagga ccgccgacgc tacatcgaga tcgtgctgga ggccgtcacc accggcgtcc
     2701 tgtcgctcga cccgctcggt cggctcacca cgctgaaccg cgcggcggcg gcgatgttcg
     2761 ggctggacgc ggccacgagc gtgggccggc ccgtggagga ggtcttcggc aacgctgacg
     2821 tccgcgacgt ggtggcgctg gtgcaccgtg tccggcgcgc ccggaccggt gcggtggagc
     2881 aggacctgcg cttgcgccgc aacggtaccg cggtctccct gctcgcctcg gccacggcgc
     2941 tccggggacc ggagggcgag tacgcgggcg ccgtggtggt cttcgacgac ctcaccgagc
     3001 tgctcaaggc ccagcggctt gccgcgtggc gcgaggtcgc gcagcggatc gcccacgaga
     3061 tcaagaaccc gctgacgcca atccagctct ccgcgcagcg gctgcgccgg cgcctcgccc
     3121 gcacggccgg cgacgagcag ctcgtgagcg agtgcaccga gacgatcatt caagaggtcg
     3181 acggcctcaa gcgcctcgtc gacgagttct cccgcttcgc gcggatgccc gtgctgacgc
     3241 cgcgtccgac ggacctgcgc ggcatcgtcg acactgtggc ggccctctac cgcgaatcgt
     3301 acccgggcct cacgctcacc acccggtacg gcgacgacct gccgctgctc gacgtcgatc
     3361 ccgaccacgt gaagcgcgcc gtgctgaacc tcgtggagaa cgccgtggac gccgtggccg
     3421 ggcagggcct ggtcgaggtc gagatcgtcc accgggccgc caccgggcgc atcgagctcg
     3481 tcgtgagcga ctccggcccg ggcatcagcg tcgaggacaa ggagagggtg ttcctgccct
     3541 acttctcgac gaagacgacg ggcatggggc tcgggctgcc catcgtctac gaggtcgtgg
     3601 ccgagcacgg cggcgccgtc cgcgtcgagg acaaccgccc gcacggcagc cgcttcgtcg
     3661 tggacttgcc cgtggcacgc accccggcgc ccctcggggc gtagcgacac ggcgccgatg
     3721 gccgacgagc acatcctcat cgtcgacgac gagcccgcca tccagtcgac cctgcgaggg
     3781 gtgctggaag acgaaggcta ccgggtgagc accgtgggca gcggccgcca ggggatcgat
     3841 ctcctcgccg acgaggcgcc ggatctcgtc ttcctcgatg tgtggatgcc ggggatggac
     3901 gggctggaca cgctggtgca gatcaagaca ctccgtccgg agaccgcggt cgtcatgatc
     3961 tcggggcacg cgacgatcga gaccgcggtc aaggccacgc ggctcggcgc ctacgatttc
     4021 atcgagaagc cgctgtcgct ggacaagacg ctgctgaccg tgtcccgggc gctcgagcac
     4081 gcgcgtctcg cgcgcgagaa tgcggccctc cgccagcgcc tcgaggaacg tctggagatc
     4141 atcggcgaca gccccgtgat gcgccgcctg cgcgacgaca tcaccacggc cgcgcccagc
     4201 aacggccgtg tcctgatcca cggcgagaat ggcagcggca aggagctcgt cgcccgcgcc
     4261 atccacgcgc tctcgtcgcg ccgggaccgt ccattcgtcg aggtgaactg cgcggcgatc
     4321 cccgaggagc tcatcgaatc ggagatgttc ggccacgaga agggcgcgtt caccggcgcc
     4381 gtggcccgcc gccgaggccg cttcgaggtg gccgacggcg gcacgctctt tctcgacgag
     4441 atcggcgaca tgagcctcaa gacccaggcg aaggtgctcc gggccctcga ggagcaggcg
     4501 ttcgagcgcg tcggggggcg cgacaagatc aaggtcgacg tgagggtcat cgcggccacc
     4561 aaccgcgatc tcgcggcgat gttcggtcgc gggttccgcg aggatctcta ctatcggctc
     4621 aatgtgatcc cgatcgaggt gccgccgctg cgcgcgcgcc gcgacgacct cccgctgctc
     4681 gtcgaccact tcatccgcgt cttctgcgcg gagaacggca agcgggtgaa gacggtgtcc
     4741 ggcgaggccc tcgcgtattt cctcgcctac gactggcccg gcaacgtacg cgagctgcgc
     4801 aacctcgtag agcggctcgt gatcatggcg ccgggcgacg tcatcggcgt cgacgacgtg
     4861 cccgcccccg tgcggcccaa ggaggcggcg ctccgggagg cagcggccgg cggcagcgcc
     4921 gacgccggcg aacgctctct gcgcgaggcg cgggacaact tcgagcgcgc ctacatcctc
     4981 accgagctgc gcgcgaacga gtggaacatg acacgcaccg ccgagcggct cgggatcgaa
     5041 cgcagccacc tgtaccgcaa gatcaaggcc tacggcatca ccccgcccaa atgagaatcc
     5101 tcgtcgtcga cgacaccgaa cccgtgcgct ttctcctcgt gcggtggctc ggcacgctgg
     5161 gtcacgaggc ggtggccgcg ggctccgggg ccgaggcgct cgcgcagctc gacgcctcct
     5221 cgttcgacgc cgt
//
LOCUS       DATIAZ010000258         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1252919, whole genome shotgun sequence.
ACCESSION   DATIAZ010000258 DATIAZ010000000
VERSION     DATIAZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252919"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VLF19_09235"
     CDS             complement(<1..473)
                     /locus_tag="VLF19_09235"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HSE93476.1"
                     /translation="MTSGRDARRDRWSAAGLAVLALGYLYAARQYPLDSLAAPGPGVF
                     PLAAGLALLVLASCQAIATLRRRWEQEHGSGAGPRSAPGHTSGAGQGSGTTDGLGTMS
                     GAESRRPRGVSRRGAPLMMALLLVVYAASVSVLGFLPASCALIVLAARLMGVSGW"
     gene            complement(483..1433)
                     /locus_tag="VLF19_09240"
     CDS             complement(483..1433)
                     /locus_tag="VLF19_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HSE93477.1"
                     /translation="MTRRIALVAVLVLAVASTAWAQAYPAKPVELTVLFGAGSAADLL
                     ARKLAEGASKDLGQPVTVVNRTGAGGAIGYNYVKGQAADGHSIVWNSNSVSTAFHAGN
                     MKIDHTAFSGVAQLTSEPVTVAVKADAPWKDIKELIAHAKAKPGAVRIGNSGRGSFTH
                     LMAVALENRAGVKFTHVPFGRELAVATVMGDKIEASAQLPAEIMAQVTGKQVRVLGVI
                     GEQRVPSLPDVPTLKENGIDLTMSLWRGVAVPKGTPEAVIARLERAFVAAARSPEFRE
                     FATKMGASVDVRSARDFDQFIARDDNDIATLMEQIGLKKQ"
     gene            complement(1444..2373)
                     /locus_tag="VLF19_09245"
     CDS             complement(1444..2373)
                     /locus_tag="VLF19_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSE93478.1"
                     /translation="MRIIHQGLPHMPPYGEFIFGHEYAGDVVAVGGTVDEFRVGDRVV
                     VEAHMGCRRCENCIRGHYTACLNFGNMKRGHRANGFTTNGGLAEYALNHVNTLFRIPD
                     SVSYEEAVVVMTAGSPLYGLERAGGYFAGETVAVLGPGPIGLMALQLVKAVGAVRVIL
                     TGTRASRLLMGKELGADVIVNAREQDPVAAVREATGGRGADVTIDCAGGDDTFDQAIK
                     MTKPGGRVILVAFYHGPVTADVADAVRRNLTIYTERGEGGTSVGRALALLAAGRVRAR
                     PLVSQTFPLTEVHRGFEVVEKRIGDPIKVIFHP"
     gene            complement(2383..2511)
                     /locus_tag="VLF19_09250"
     CDS             complement(2383..2511)
                     /locus_tag="VLF19_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93479.1"
                     /translation="MSLPRTMRATVLTAPHRIELQERPVPQLGPEDVLVRVRACGV"
     gene            2675..3367
                     /locus_tag="VLF19_09255"
     CDS             2675..3367
                     /locus_tag="VLF19_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSE93480.1"
                     /translation="MSGWRGWWLATLVVTFVLAATRDSSVAGGREPTSRDLDLITTAA
                     SGNTARVKRLLVAGASVRAADARGVTALIAAAYRNHVHVARVLIQAGADVNAQDRTRQ
                     SAFLIATSEGYVELLRLTLEGGADVRRTDSYDGTGLIRAAHHGHVEVVRELLRAKTPI
                     DHVNRLGWTALLEAIILGDGGPRHTETVRLLVEAGADVNLADGDGVTPLAHARRRGQT
                     RIVEILERAGAK"
     gene            3393..>4186
                     /locus_tag="VLF19_09260"
     CDS             3393..>4186
                     /locus_tag="VLF19_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HSE93481.1"
                     /translation="MKTTRARRESSSPWPKGAKCAVMLTFDFDAETLWLARDPKSIER
                     PGVMSQGRYGARVGVPRLLALLRAEGVPATFFIPGWVVEHHGDQARAIRDAGHEIGHH
                     GYRHDPADPEKPDVERAAFEQGMKALESVLSIRPRGYRAPSWELTPITLALVRQHGMF
                     YSSNMMDDVHPYLHSGEPPVVELPVQWVLDDAPYLLMHPRLAPRPMQSPQAVFGIWRD
                     EFLGFHEWGGLFNLTCHPQVIGHPSRLLMLRRLIRFVKGHRGVWWTT"
BASE COUNT          590 a         1514 c         1494 g          588 t
ORIGIN      
        1 caccagccgg aaacgcccat gagccgcgcg gcgagcacga tgagagcgca cgaggcgggg
       61 aggaagccga gcacgctcac gctcgcggcg tagacgacga gaagaagggc catcatcagc
      121 ggcgcccccc gccgggagac gccacggggc cggcgggact ccgctccgga catcgtgccc
      181 aggccgtccg tggtgcccga gccttgtccc gcgcccgagg tgtgtcctgg ggccgagcga
      241 ggccccgcgc ccgagccatg ctcctgttcc catcggcggc gcagcgtggc gatcgcctgg
      301 cagctcgcga ggacgaggag ggcgagcccg gcggcgaggg gaaacacgcc gggacccggg
      361 gcggccagcg agtcgagagg gtactggcgg gcggcataga ggtagccgag ggcgaggacg
      421 gcgaggccgg ccgcgctcca tcggtcacgg cgcgcgtcgc gacccgacgt catgccggcg
      481 cgctactgct tcttgagccc gatctgctcc atcagggtcg cgatgtcgtt gtcgtcgcga
      541 gcgatgaact gatcgaagtc tcgcgcgctg cggacgtcga ccgacgcgcc catcttcgtg
      601 gcgaactcgc ggaactccgg gctccgcgcg gccgcgacga acgcccgctc cagacgcgcg
      661 atgaccgcct ccggcgtgcc cttcgggacg gcgacgcctc gccagagcga catggtcaga
      721 tcgatcccgt tctccttgag cgtcggcaca tcgggcaggc tcggcacgcg ctgctcgccg
      781 atgacgccga gcacgcggac ctgcttgccc gtcacctggg ccatgatctc cgccgggagc
      841 tgcgcgctcg cctcgatctt gtcacccatc acggtcgcga ccgccagctc gcgcccgaac
      901 ggcacgtggg tgaacttgac gccagctcgg ttctcgagcg ccacggccat gaggtgcgtg
      961 aagctgccgc ggcccgagtt gccgatgcgc accgctccgg gcttggcctt cgcgtgcgcg
     1021 atcagctcct tgatgtcctt ccagggcgcg tcggccttga cggcgacagt gacaggctcg
     1081 ctggtgagct gggccacgcc cgagaacgcg gtgtggtcga tcttcatgtt gcccgcgtga
     1141 aaggcggtgc tgacgctgtt ggagttccag acgatcgagt gaccgtcggc ggcctggccc
     1201 ttcacgtagt tgtagccgat ggcgccgccg gcgccggtgc ggttgacgac ggtgaccggc
     1261 tggccgaggt ccttcgaggc gccctcggcg agcttgcgcg cgagcagatc ggcggcgctc
     1321 ccggcgccga acagcacggt cagctcgacc ggcttcgcgg ggtacgcctg cgcccacgcc
     1381 gtcgacgcga cggccaggac caggacggcg accagggcga tccgtcgtgt catcggtgcc
     1441 ccctcacgga tggaagatga ccttgatggg gtcgccgatc cgcttctcga cgacctcgaa
     1501 accacggtga acttcggtca gcgggaaggt ctggctcacc agcggtctgg cccgcacgcg
     1561 cccggccgcc agcagcgcca gcgcgcggcc cacgctcgtg ccgccctcgc cgcgctcggt
     1621 gtagatggtc aggttgcggc gcacggcgtc ggccacgtcg gcggtgacgg gcccgtggta
     1681 gaacgccacc aggatgacgc ggccgccggg cttcgtcatc ttgatcgcct ggtcgaaggt
     1741 gtcgtcgccg ccggcgcagt cgatggtcac gtcggcgcca cggccgccgg tggcctcgcg
     1801 cacggccgcc accgggtcct gctcgcgcgc gttgacgatg acgtccgcgc ccagctcctt
     1861 gcccatcagg agccgcgacg cccgcgtgcc ggtgaggatc acgcgcacgg cgccgaccgc
     1921 cttgaccagc tgcagggcca tgagcccgat cggcccggga cccagcacgg cgacggtctc
     1981 gccggcgaag tagccgcccg cacgctcgag gccgtagagc gggctgcccg ccgtcatcac
     2041 caccaccgcc tcctcgtagg agacgctgtc ggggatgcgg aagagcgtgt tgacgtggtt
     2101 cagcgcgtac tcggcgagcc cgccgttcgt ggtgaacccg ttggcgcggt gcccgcgctt
     2161 catgttgccg aagttgagac acgccgtgta atggccgcgg atgcagttct cgcaccggcg
     2221 gcaacccatg tgcgcctcga cgaccacccg gtcgcccacc cgaaactcgt ccaccgtgcc
     2281 gcccaccgct accacgtctc ccgcgtactc gtgaccgaag atgaactccc cgtagggggg
     2341 catgtggggc agcccctggt ggatgattct caggtccgtg cctcagacgc cgcaggcgcg
     2401 cacgcggacc agcacgtctt ccggcccgag ctggggcacg gggcgctcct gcagctcgat
     2461 ccggtggggc gccgtcagca ccgtcgcccg catggtcctc ggaaggctca ttcgcgattc
     2521 ctttccgccg ggcgggggac cccggcgaca gcagggtagc acagcgctgc gcggagcccg
     2581 accgccgcgc tacactcgga gaccctggag gaagcgaatg tgcggctggg cccgcgcagt
     2641 ggccgcgcga ggcgagcctc gtggcggtga gccggtgagc gggtggcggg gctggtggct
     2701 cgccacgctc gtcgtcacct tcgtcctcgc ggccacacgt gactcgtccg tggccggcgg
     2761 acgcgagccg acctcacgcg atctcgacct gatcacgacg gcggcgagcg gcaacacggc
     2821 cagggtgaag cggctcctcg tcgccggcgc ctccgtccgc gctgccgacg cgcggggcgt
     2881 caccgccctg atcgccgcgg cttatcgcaa ccacgtgcac gtcgcgcgag tcctgatcca
     2941 ggccggcgcc gacgtcaacg cgcaggaccg gacgcgccag agcgcgttcc tgatcgcgac
     3001 gtcggagggc tacgtggagc tgttgcgatt gacgctggag ggcggcgccg acgtgcggcg
     3061 caccgactcg tacgacggca ccggcctgat ccgcgcggcc caccacggtc acgtcgaggt
     3121 cgttcgcgag ctgctccgcg cgaagacccc catcgaccac gtcaaccggc tgggctggac
     3181 ggcgctgctc gaggccatca tcctgggcga cggcgggccg cggcacaccg agacggtgcg
     3241 gctcctggtc gaggccggcg ccgacgtgaa cctcgccgac ggcgacggcg tgaccccgct
     3301 ggcccacgcc cggcggcgcg gccagacgcg catcgtggag atcctcgagc gcgccggggc
     3361 gaagtagccg gcgccatgtg gaggggagcc ccatgaagac cacgcgagcc aggcgcgagt
     3421 cgtcgtcgcc gtggcccaag ggcgcgaagt gtgccgtcat gctgaccttc gacttcgacg
     3481 ccgagacgct ctggctcgcg cgcgatccga agtcgatcga gcggccgggc gtgatgtccc
     3541 agggccgcta cggcgcccgg gtcggcgtgc cgcgcctgct ggcgctcctg cgcgcggagg
     3601 gcgtgccggc gacgttcttc atccccggct gggtggtgga gcaccacggc gaccaggcca
     3661 gggccatccg cgacgccggc cacgagatcg gccaccacgg ctaccgccac gatccggccg
     3721 atcccgagaa gcccgatgtc gagcgcgcgg cgttcgagca agggatgaag gcgctcgaga
     3781 gcgtgctgag catccgcccg cgcggctacc gcgcgccgtc gtgggagctg acacccatca
     3841 cgctggcgct cgtgcgccag cacggcatgt tctactcgtc gaacatgatg gacgacgtcc
     3901 acccgtacct gcactccggc gagccgccgg tggtggagct gccggtgcag tgggtgctcg
     3961 acgacgcgcc ctacctcctc atgcacccgc gcctggcccc gcggccgatg cagagcccgc
     4021 aggccgtgtt cggcatctgg cgcgacgagt tcctcggctt ccacgagtgg ggcggcctct
     4081 tcaacctcac gtgccacccg caggtgatcg gccacccctc gcgcctgctg atgctgcgcc
     4141 ggctcatccg cttcgtgaag ggccaccgcg gcgtctggtg gaccac
//
LOCUS       DATIAZ010000259         4471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1515553, whole genome shotgun sequence.
ACCESSION   DATIAZ010000259 DATIAZ010000000
VERSION     DATIAZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515553"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(403..1062)
                     /locus_tag="VLF19_09265"
     CDS             complement(403..1062)
                     /locus_tag="VLF19_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93482.1"
                     /translation="MSRRERIGWAIATALALAIPIAVLVAVAASPATEQLRASIDQIL
                     RVVSDSDLKKPGRAAERRAAIRKVALEIFDVQEITKRVCGLHWSARTPAEREELVGLF
                     RELLERAYLSRIELYNGERIAFTGETVDGDVATVRTRLTLREGVEVPVEYRMMRRGER
                     WVSYDVVIEGVSLVANYRGQFNRILQGGSYAELVRRLRAMLEGHSEAGGAASRRITQA
                     R"
     gene            complement(1059..1217)
                     /locus_tag="VLF19_09270"
     CDS             complement(1059..1217)
                     /locus_tag="VLF19_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93483.1"
                     /translation="MNRLLQAIQAMLQHEATIGWIDSFLLCLGFTCTVASTAPALARI
                     CRRPGDRS"
     gene            complement(1451..2029)
                     /gene="thpR"
                     /locus_tag="VLF19_09275"
     CDS             complement(1451..2029)
                     /gene="thpR"
                     /locus_tag="VLF19_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HSE93484.1"
                     /translation="MAAIGSIRSFVAVLLPDAVRAALAAEIERLRAAARNVDWVAADN
                     LHVTLKFLGSVAPGRLADTEAALGRVAAAAPPFDLTLGGIGAFPTSSRPRVIWAGVLG
                     GRDALGTLSGRVHDALADLGFEREARAYSPHVTLGRVRAPRRDAALAAVIEAAAGRGF
                     GTLRVNRLVLMRSDPSPRGSRYTALVSWPLGP"
     gene            complement(2017..2865)
                     /locus_tag="VLF19_09280"
     CDS             complement(2017..2865)
                     /locus_tag="VLF19_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93485.1"
                     /translation="MRRRWLAVLALASAASGCAPTATPMERGLSLYDQGYYLSAVMAF
                     DEAVRQSPESSMAHTNRGVARMRAGDTRGALEDFTRASELSPRDAEVWFNRGNVNVAQ
                     NDLAAAIADFTRAVELKPDFSRAFFNRGIARLRGGDEDGARADWRRAVAAERDPKTRE
                     AMSRAAGLADDSAPTAGPPSAAPAPRVADPGPAGTPPADAPGPAIPTLRPVTATGLVA
                     RDQGSPAAPPALDPRALANRALDRELSGDREGAIRDLLAALDIERDPQRRAGIENLLR
                     LLQSPR"
     gene            complement(2862..4112)
                     /locus_tag="VLF19_09285"
     CDS             complement(2862..4112)
                     /locus_tag="VLF19_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HSE93486.1"
                     /translation="MTPGAIVVGLGAAGPTIDEAAATAAAVLAGHGLPAAARVLVEDD
                     EAALDCALSQKTALTVLVAGPGGSSGDVLRRALARLAGVRLVLSERMLAAQEEASRRR
                     DRPLARRADRQALLPQGATVLPVADGEPAWSLAVGPRLFLVVPFGAALATTLEQHLAP
                     LAQARLGGRQAGVVRTLRLAGVTLADVEERLAEWLGSGAAAGEVEVSTLPGEGEVWVR
                     LRARGATPTAASEALTAVEAKLAPVLAHDCYGRDGDTLERVVGRLLVERRLTLALAES
                     CTGGLVGHRITSVPGSSAYFERGVMVYSNRAKEEMLGVSPEILRTHGAVSAPCAEAMA
                     RGICTASGAACGVSVTGIAGPDGGSPAKPVGTVFVGVAVAGEVSARRFGFSGDRASIK
                     WQSAQAALDLLRRALLARPVEAPR"
     gene            complement(4109..>4471)
                     /locus_tag="VLF19_09290"
     CDS             complement(4109..>4471)
                     /locus_tag="VLF19_09290"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HSE93487.1"
                     /translation="AILWLVPFSRAGLALFFVAVTLAGIWAAHRAERLLRAKDPGAIV
                     IDEIAGMTLSVLVFPITPGVLVAAFVLFRLFDVVKPPPARASQQLGGGIGVMIDDLVA
                     GLYALGGLAALRAVAGWP"
BASE COUNT          632 a         1681 c         1606 g          552 t
ORIGIN      
        1 tccagcggcg ccccggagcc cagccgcatg acggcgccct tgccgaactg ccgctcgatc
       61 tgggtgatcg ccaggtccag ggcctgccgc cggtcgctct tcggctccgc catccttggc
      121 ctcctgattc gccgtcgggg caccttcgca gggctccggt aacccaacgt accgccctgg
      181 accgtgctgc ctcgtgtcgc ccgtcggagc gctttcggcg ggctccggcg ctcgaggttc
      241 agcccctgga ccaccccgcc gatctcgcgc tcgcgcgcgt catctgcgct tcgagcattc
      301 taggagtcgc gtcgggtggc gtcaatgttc gtggaacgat acggcgcgcc ccggggcgaa
      361 ccggcgcgcg cggaggcagg cgggcgaggg cgctgtgggg ggctaccggg cctgcgtgat
      421 gcgacggctg gcggcgcctc cggcctcgga atgcccctcg agcatggcgc gcaggcggcg
      481 caccagctcg gcgtaggagc cgccctggag gatgcggttg aactggcctc ggtagttggc
      541 gacgagactc accccttcga tgaccacgtc gtaggacacc cagcgctcgc cccgccgcat
      601 catgcggtac tccaccggga cctcgacgcc ctcccggagc gtaaggcggg tgcgcacggt
      661 ggcgacgtca ccgtccaccg tctcgcccgt gaaggcgatg cgctcaccgt tgtagagctc
      721 gatcctggag aggtaggcac gctcgagcag ctcgcggaag aggcccacga gctcttcgcg
      781 ctcggcgggc gtccgggcgc tccagtgcag cccgcagacc cgcttcgtga tctcctggac
      841 gtcgaagatc tccagggcaa ccttccggat ggcggcccgc cgctcggccg cgcgcccggg
      901 cttcttgagg tcggaatcgc tcacaacgcg caggatctgg tcgatcgacg cgcgcagctg
      961 ctccgtcgct ggcgaggccg ccacggccac caggacggcg atcgggatcg ccagcgcgag
     1021 ggcggtcgcg atcgcccagc cgattcgctc gcggcggctc atgaccgatc tccgggccgg
     1081 cggcagatcc tcgcgagcgc cggcgccgtg ctggcgaccg tgcacgtgaa gccgaggcac
     1141 agcaggaagc tatcgatcca tccgatcgtc gcctcgtgct gcagcatcgc ctggatggcc
     1201 tggagcagtc ggttcattgg ctggcccccc tgactgcaca ccgtctgggc aagtcctctg
     1261 ccaagcctgg ggggggctcc gacagccgcg tggcacacca agatccacac atgttggacg
     1321 ctagttgctg atcagactcc cgtcttgcgt atgagccgat ggggagaaat agccaggctc
     1381 cctggcttcc ggtgtatgag gatctcccgg gagattgcgt caggcaggga ggctggcctg
     1441 gtcgagggga ctacggcccc agcggccagg acacgagagc ggtgtagcgg gacccgcggg
     1501 gggaaggatc gctgcgcatc aggacgaggc gatttacccg cagcgtgccg aagccgcgac
     1561 cggcggcagc ctcgatgact gcggccaggg cagcatcgcg gcggggcgcc cgcacccgcc
     1621 cgagcgtcac gtgcggcgag tatgcccgcg cctcccgctc gaagcccagg tcggccaggg
     1681 cgtcgtgcac gcgcccggag agcgtgccga gcgcgtcgcg gccacccagc accccagccc
     1741 agatcacccg cggacgggag gacgtgggaa acgcgccgat tccgccgagg gtcagatcga
     1801 acggcggggc ggcggcggcg actcgcccca gggccgcctc cgtatcagcc agtcgcccgg
     1861 gcgccacgct gccgaggaac ttgagcgtca cgtgcagatt gtcggcggcg acccagtcga
     1921 cgttccgggc ggcagcccgc aggcgctcga tctcggcggc gagtgccgcg cgcaccgcat
     1981 cgggcagcag gacggcgacg aacgagcgga tcgaccctat cgcggcgatt gcagcaggcg
     2041 cagcaggttc tcgatgccgg cacggcgctg ggggtcgcgc tcgatgtcca gcgcggccag
     2101 caggtctcga atcgctccct cgcggtcgcc gctcagctcg cggtcgagcg cccggttggc
     2161 gagcgcgcgc gggtccagcg ccggcggcgc ggcaggcgaa ccctgatccc tcgcgaccag
     2221 tccggtcgcg gtcacgggcc gcagagtcgg aatcgcgggc ccgggcgcgt ccgccggcgg
     2281 cgtcccggcc ggccccggat ccgcgacccg tggcgccggc gccgcggaag gcggcccggc
     2341 ggtcggcgcc gaatcgtcgg ccaaacccgc cgcgcgcgac atcgcctcgc gcgtcttcgg
     2401 gtcgcgctcg gccgcgaccg cccgccgcca atccgcacgc gcaccgtcct cgtcgcctcc
     2461 gcggagccgc gcgataccgc ggttgaagaa ggcccgcgag aaatctggct tcagctcgac
     2521 ggcgcgggtg aagtccgcga tggcggcggc gagatcgttc tgcgccacgt tgacgttccc
     2581 gcggttgaac catacctcgg cgtcgcgggg gctcagctcg ctcgcccggg tgaagtcctc
     2641 gagcgcgccc cgcgtgtcgc ccgcgcgcat ccgcgccact ccgcggttcg tgtgggccat
     2701 cgaggattcc ggcgactggc gcacggcctc gtcgaaggcc atgaccgccg agaggtagta
     2761 accctggtcg tagagcgaca ggccgcgctc catcggggtc gccgtcggcg cgcatccgga
     2821 cgccgcggac gccagcgcca ggacggccag ccagcggcgc ctcatcgcgg agcctccacc
     2881 ggccgggcga gcagcgcgcg gcgaagcagg tcgagcgcag cctgggcgga ctgccacttg
     2941 atcgaggcac gatcgccgga gaacccgaag cgtcgcgcgc tgacctcgcc ggcgacggcc
     3001 acaccgacga acaccgtgcc gacgggcttg gccggggagc cgccgtcggg accggcgatg
     3061 ccggtcaccg agacgccgca cgcggccccg ctcgccgtgc agatcccgcg cgccatggcc
     3121 tcggcgcagg gggcactgac cgctccgtgc gttctcagga tctcgggtga cacgccgagc
     3181 atctcctcct tggcacgatt cgagtagacc atcacgcccc gctcgaagta cgccgacgag
     3241 cccggcacgc tcgtgatccg gtggccgacg agcccgccgg tgcacgactc ggccagcgcg
     3301 agcgtgaggc ggcgctcgac gagcagacgg ccgaccacgc gctcgagcgt gtcgccgtca
     3361 cggccgtaac agtcgtgtgc gagcacgggt gcgagcttcg cctccaccgc ggtcagcgcc
     3421 tcgctcgccg ccgtcggcgt cgcgcctcga gcacgaagcc ggacccacac ctcgccctcc
     3481 ccgggcagcg tgctcacctc cacctcgccc gccgcggcac cggagccgag ccactcggcg
     3541 aggcgttcct cgacgtcggc gagcgtgacg cccgccagcc gcagcgtacg gacgacaccg
     3601 gcctgccggc cgcccagccg cgcctgcgcg agaggcgcca ggtgctgctc cagggtcgtt
     3661 gccagcgccg ccccgaacgg cacgacgagg aagaggcgcg gaccgaccgc cagcgaccag
     3721 gcgggctcgc catccgcaac cggcaggacc gtcgccccct gcggcagcag cgcttgccgg
     3781 tcggcccgtc gcgcgagcgg gcggtcgcga cgccgcgagg cttcctcctg cgccgccagc
     3841 atccgctcgc tcagcaccag ccgcacgccc gccagccgcg cgagcgcccg gcgcaggacg
     3901 tcgcccgacg agccgccggg gccggcgacg agcacggtga gcgcggtctt ctggctcagc
     3961 gcgcagtcga gcgccgcctc gtcgtcctcc acgagcaccc gcgccgcggc cgggagcccg
     4021 tggccggcga gcaccgcggc cgccgtggcc gcggcctcgt cgatcgtcgg acccgccgcc
     4081 ccgaggccga ccacgatcgc gccgggggtc acggccagcc cgccaccgcg cgcagggccg
     4141 cgaggccgcc gagcgcgtag agtccggcga cgaggtcgtc gatcatcacg ccgatgcccc
     4201 cgcccagttg ctggctcgcg cgcgccggcg gcggcttgac gacgtcgaac agccggaaca
     4261 ggacgaatgc ggccacgagc acgccggggg tgatggggaa caccaggacc gagagcgtca
     4321 tccccgcgat ctcgtcgatg acgatcgcgc cgggatcctt cgcccggagc aggcgctccg
     4381 cgcggtgcgc cgcccagatg ccggcgagcg tgaccgcgac gaagaagagc gcgaggccgg
     4441 cgcgcgagaa cggcaccagc cacaggatcg c
//
LOCUS       DATIAZ010000260         5011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_727153, whole genome shotgun sequence.
ACCESSION   DATIAZ010000260 DATIAZ010000000
VERSION     DATIAZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727153"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VLF19_09295"
     CDS             complement(<1..1277)
                     /locus_tag="VLF19_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93488.1"
                     /translation="MSAPPAAIQVHALTKRYGAVHAVRGIDLEVRTGEIFGLIGPDGA
                     GKTSTFQILGGVMEATSGTAELLGQPARDARAAVGYLTQAFSLYQDLSVAENLRYVGE
                     LRRVPGAEIERRGQRYLTIFGLDRFTGRLAGRLSGGMKQKLALACALVTEPRILLLDE
                     PTTGVDPVSRREFWEALVQLSSQGITILLATPYLDEAERCHRVALMHEGRIHQTGAPG
                     ELRHRLGLQRLEVRTDSLRQAAGALTGGDGIVDVQRFGDRLDVMVRDVHEGERSVRAA
                     LAAARLAVHGVRAATPTLENTFVSILRGLEGEAVVPEFPGRRHDGAVEAAVAIGARGL
                     RRTFGTFEAVKGIDLEVRYGEVYGLLGANGAGKTTTIKMLCGLLEPSAGQVQLAGERG
                     QLRSEGVRQQVGYMSQKFSLYDDLTVEENLDFFA"
     gene            complement(1274..2467)
                     /locus_tag="VLF19_09300"
     CDS             complement(1274..2467)
                     /locus_tag="VLF19_09300"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSE93489.1"
                     /translation="MISRRRPLVIAVLLVVLGLAGFAAWRLFLAPSDLPPGVIAVSGR
                     IEGDDAAVAAKATGRVREITVREGDRIEAGHVIAVLDDAQIRAREQQAAAAVRQAEAR
                     VRLAQHQITVLNEQLRQGEFGVDQARADAQGRVSEAEARLAAAEAQLAQAEASHAQAK
                     WDREAFARLFQKELIAEQEARRAQSNEEAQAAVVAAARRQVEAARGALTAARANLVNP
                     AIRSSQVAAVQGQILQAQADIAAAQADAERARAQLDEARANRQDLQVVAPFAGTVATR
                     TAEPGEVVMAGTPVVTLVNLAEVYLRAFVPEGQIGRVRVGQPARVYIDSAPAQPIEAI
                     VSRVDPEASFTPENTYFREDRVKQVVGVKLQLRGAVGFAKPGMPADGEILVDGGEWPT
                     RQARR"
     gene            complement(2464..3141)
                     /locus_tag="VLF19_09305"
     CDS             complement(2464..3141)
                     /locus_tag="VLF19_09305"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSE93490.1"
                     /translation="MTPASQTSSRRGRRRDAAGTREALLAAGTELFAERGYEGVPVWT
                     IAQKAGVNKAMINYHFGGKRKLYLTIVSATFAEIVSSVERLADSPRPAPDLLRDLIAV
                     VGEVVTRRHPHVCTMMLREVLAGGRHLEPELIDKPVRILTAVQRIVARGVREGAFRPV
                     DPLLTHLSLVGSLVFFFATRRFRERVLADRRLEMTPPDAGAYVKHIQDLLAHGLAAHG
                     PSPKRGT"
     gene            3248..4051
                     /locus_tag="VLF19_09310"
     CDS             3248..4051
                     /locus_tag="VLF19_09310"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSE93491.1"
                     /translation="MDEAFALLARWGYVILAAVVVAEQVALPIPAVPVLLGIGALAAD
                     GRMSIVAAVAIACGVAVPIDLLWRRVGASRGIRVLRFMCRLSLEPSSCVRRTETLFEQ
                     YGSRALLVAKFVPGLTALAPALAGMVRVPLGRFLILDLAGVVLWAGAWASLGFLFSRA
                     LEFILAQVSRAGSLLGILAMGLMTYVLLKMIRRQRFLRRLRMARITASSLKEMLDRGE
                     EVWVADLRSALDVEMSPYTIPGARWIAADAISRSPADIPRDREIVLVCT"
     gene            complement(4288..4530)
                     /locus_tag="VLF19_09315"
     CDS             complement(4288..4530)
                     /locus_tag="VLF19_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93492.1"
                     /translation="MNLFRSEGHARRWSAFQARAEEGFIPLVALAGFFGTESRRHMLD
                     ADYLSTWFPRRAAERRAYLEQIGKTTPFWLGTPDVV"
     gene            complement(4537..>5011)
                     /gene="merB"
                     /locus_tag="VLF19_09320"
     CDS             complement(4537..>5011)
                     /gene="merB"
                     /locus_tag="VLF19_09320"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HSE93493.1"
                     /translation="GTMADPRLMDGMFQRIMRGLVDTGRAPHYAELARALGIPVDDGR
                     VVLHEVMQAYPIGWLHPETDYIASFPPLNNLPTQYRVTVRGEQRWFAQCGFEATSVTW
                     LFPGETVRIDAPCLDCGDPVTVEMRDGRITWVDPPAVVGHLNYGFGPSRGKPQFL"
BASE COUNT          690 a         1778 c         1785 g          758 t
ORIGIN      
        1 gcgaagaagt ccaggttctc ttccaccgtg aggtcgtcgt acagcgagaa cttctgcgac
       61 atgtagccca cctgttgccg gacgccctcc gagcgcagct gtccgcgctc gcccgcgagc
      121 tgcacctgcc cggcgctcgg ctcgaggagg ccgcagagca tcttgatcgt ggtcgtcttg
      181 cccgcgccgt tggcgccgag gaggccgtac acctcgccgt agcgcacctc gaggtcgatg
      241 cccttgacgg cctcgaaggt gccgaacgtc cggcgaaggc cgcgggcgcc gatggcgacg
      301 gcggcctcga ccgcaccgtc gtggcgtcgg ccggggaact cggggaccac cgcctcgccc
      361 tcgagaccgc gcaggatgga gacgaacgtg ttctcgagcg tgggcgtcgc ggcccgcacg
      421 ccgtgcaccg cgagccgggc ggcggccagt gccgcgcgga ccgagcgctc cccctcgtgg
      481 acgtcgcgga ccatcacgtc gaggcgatcg ccgaagcgct ggacatcgac gattccgtcg
      541 cctcccgtca gggcgcccgc ggcctggcgc aggctgtccg tgcgcacctc caggcgctgg
      601 aggccgaggc ggtggcgaag ctcgcccggc gccccggtct ggtggatgcg cccctcgtgc
      661 atgagcgcga cccggtggca tcgctcggct tcgtcgaggt acggggtcgc caggaggatc
      721 gtgatgccct ggctggagag ctggacgagc gcctcccaga actcgcgacg ggagaccgga
      781 tcgacgcccg tcgtgggctc gtcgaggagg aggatgcgcg gctcggtcac gagcgcgcac
      841 gcgagggcca gcttctgctt catgccgccg ctgaggcggc cggccagccg gccggtgaag
      901 cgatcgaggc cgaagatcgt gaggtagcgc tgcccgcgcc gctcgatctc ggcgcccggc
      961 actcgccgga gctcacccac gtagcggagg ttctcggcca cgctcaagtc ctggtagaga
     1021 ctgaacgcct gggtcagata gccgacggcg gcgcgcgcgt cccgcgccgg ctgcccgagc
     1081 agctcggccg tgccggaggt cgcctccatc acgccgccca ggatctggaa ggtcgacgtc
     1141 ttccctgccc cgtccggccc gatgagcccg aagatctcgc cggtccgcac ctcgaggtcg
     1201 atgccgcgga ccgcgtgcac ggcgccgtag cgcttcgtca gcgcgtggac ctggatggcg
     1261 gccggcggcg cgctcatcgg cgagcctgac gggtgggcca ttcgccgcca tcgacgagga
     1321 tctcgccgtc ggccggcatg ccgggcttcg cgaagccgac ggcgccgcgg agctggagct
     1381 tcacgccgac cacctgcttc acccgatcct cccggaaata cgtgttctcc ggcgtgaacg
     1441 acgcctcggg gtcgacgcgc gacacgatag cctcgatggg ctgagccggc gccgagtcga
     1501 tgtagacccg tgccggctgt cccacgcgga cgcgcccgat ctgaccctcg gggacgaatg
     1561 ccctcaggta cacctcggcc aggttcacga gcgtgacgac gggggtgccg gccatgacca
     1621 cctcgccggg ctcggccgta cgtgtggcga ccgtgcccgc gaagggcgcc acgacctgga
     1681 gatcctggcg gttggcccgc gcctcgtcga gctgagcgcg tgcacgctcg gcatccgcct
     1741 gggcggcggc aatgtccgcc tgagcctgga ggatctgccc ctggacggcg gcgacctgcg
     1801 aggagcggat ggctgggttg acgagattgg cccgcgccgc cgtgagcgcg ccgcgtgcag
     1861 cctcgacctg gcgccgcgcg gccgcgacca ccgccgcctg ggcttcctcg ttgctctggg
     1921 cccgtcgcgc ctcctgttcg gcgatcagct ctttctggaa gagacgcgcg aacgcctccc
     1981 ggtcccactt cgcctgcgcg tgggaggcct cggcctgggc cagctgggcc tcggccgccg
     2041 ccagccgcgc ctcggcctcg ctgacacggc cctgcgcatc cgcgcgcgcc tggtccacgc
     2101 cgaactcgcc ctgccggagc tgctcgttga gcaccgtgat ctggtgctgg gccaaccgga
     2161 cgcgggcctc cgcctgccgg acggcagccg ccgcctgctg ctcgcgggcg cggatctgcg
     2221 cgtcgtcgag cacggcgatg acgtggccgg cctcgatccg atctccttcc cgcacggtga
     2281 tctcgcggac ccgcccggtg gccttcgcgg cgacggcggc gtcgtcgccc tcgatccgtc
     2341 cgctcaccgc gatcacgccc ggtgggaggt cggagggcgc gaggaacagg cgccaggcgg
     2401 cgaacccggc gaggcccagc acgaccagca gtacggcgat cacgagcggg cgccggcgcg
     2461 agatcatgtg cctcgtttgg ggctcggccc gtgagccgcg agcccgtggg caagcaggtc
     2521 ctggatgtgc ttcacgtagg cgccggcgtc cgggggcgtc atctcgaggc gccgatccgc
     2581 gagcacgcgc tcgcggaagc ggcgcgtggc gaagaagaac acgaggctgc ccacgaggct
     2641 caggtgggtc agcaggggat cgacgggccg gaaggcgccc tcacgcaccc cgcgggccac
     2701 gatgcgctgc accgcggtga ggattcgcac gggcttgtcg atcagctcgg gctcgaggtg
     2761 cctgccgccg gccaggacct cgcgcaacat catcgtgcag acgtgggggt ggcgtcgtgt
     2821 caccacctcg cccacgacgg cgatcagatc ccggagcaga tccggcgccg gccgcggcga
     2881 gtcggcgagg cgctccacgc tcgacacgat ctccgcgaac gtggcgctca cgatcgtcag
     2941 gtagagcttc cgcttgccgc cgaagtgata gttgatcatc gccttgttga ccccggcctt
     3001 ctgggcgatc gtccagaccg gcacgccctc gtagccgcgc tcggcgaaga gctcggtgcc
     3061 ggcggcaagc agggcctcgc gggtcccggc ggcgtctcga cgccggccgc ggcggctact
     3121 ggtctgagat gcgggcgtca tccaaagtct cctcgcaggc tcatgtcaac taaacgtatg
     3181 gttaattatc ccacgccgtg tcaagaggtg gccagtccgg gacgcacttg atggagaatg
     3241 cacggccatg gacgaggcgt tcgccctcct cgctcgctgg ggatacgtca tcctcgcggc
     3301 ggtcgtggtg gccgagcaag tcgcgttacc gatcccggcc gtgccggtgc tgctcggcat
     3361 cggcgccctg gccgccgacg gacgcatgag catcgtcgcc gcggtggcca tcgcgtgtgg
     3421 ggtggcggtg ccgatcgact tgctctggcg ccgagtcggc gccagtcggg gaatccgggt
     3481 gttgcgcttc atgtgtcgcc tgtccctgga gccctcctcc tgcgtccggc gcaccgagac
     3541 cctgttcgag cagtacggca gtcgggcgct cctcgtggcg aagttcgtcc ccggactcac
     3601 cgctctcgcg cccgcgctcg ccgggatggt ccgggtcccc ctggggcggt tcttgatcct
     3661 cgacctcgcg ggcgtggtgc tgtgggccgg cgcgtgggcg tcgctcggat ttctcttcag
     3721 ccgggcgctg gagttcatcc tcgcgcaggt ctctcgggca gggagtctgc tcgggatcct
     3781 ggcgatgggc ttgatgacct acgtgctgct caagatgatc cggcgccaac gcttcctgcg
     3841 tcggcttcga atggcccgga tcacggcctc gagcctgaaa gagatgctcg accgcggaga
     3901 ggaggtgtgg gtcgcggatc tacgttcggc cctggacgtg gagatgtcgc cctacaccat
     3961 tcccggagcg cgatggatcg cggccgacgc gatcagccga tcgccggcag acatccctcg
     4021 cgatcgcgag atcgtcctcg tctgcacgtg accggacgag gcgacgagcg cccgggtggc
     4081 gctggcgctc aagcgacagg gcgtcacacg cgttcggccc ctgctcggtg ggctggatgc
     4141 ctggctggag cgccagtatc ccgtcgagcg gctcgagccc gccccccgga cgacgccgac
     4201 cgcctgacag tccccatcgc ctcagcgcgt ggcgtggctc ggctcccccg gtgccctcga
     4261 tccacagccg gacggtttgc gccgccttca tacgacatcc ggcgtcccga gccagaaggg
     4321 agtcgtcttg ccgatctgct cgagataggc gcgacgctcc gccgcccgcc ggggaaacca
     4381 cgtcgacagg taatcggcgt ccagcatgtg gcggcgcgac tcggttccga agaaccccgc
     4441 gagggcgacg agcgggatga agccctcctc ggcgcgggcc tggaacgcgg accaccggcg
     4501 ggcgtgccct tccgaccgga agaggttcat gcccgttcag aggaattgcg gcttccctcg
     4561 cgacgggccg aagccgtagt tcagatggcc gacgaccgcg ggcggatcga cccacgtgat
     4621 ccggccgtcg cgcatctcga cggtcacggg gtcaccgcag tcgaggcagg gggcgtcgat
     4681 gcgaaccgtc tcgccgggaa acagccacgt cacggaggtc gcctcgaagc cgcactgggc
     4741 gaaccaccgc tgctccccgc gcaccgtgac gcgatactgc gtgggcagat tgttgagcgg
     4801 cgggaacgac gcgatgtaat ccgtctcggg gtggagccac ccgatcggat aagcctgcat
     4861 cacctcgtgg agcacgacgc gcccgtcgtc gaccggaatc cccagcgcgc gcgcgagctc
     4921 ggcgtagtgg ggcgcgcgcc ccgtgtccac gagcccgcgc atgatgcgct ggaacatccc
     4981 gtccatgagg cgtggatcgg ccatcgtccc c
//
LOCUS       DATIAZ010000261        14333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_333567, whole genome shotgun sequence.
ACCESSION   DATIAZ010000261 DATIAZ010000000
VERSION     DATIAZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14333
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333567"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..241
                     /locus_tag="VLF19_09325"
                     /pseudo
     CDS             9..241
                     /locus_tag="VLF19_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006623181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(473..1510)
                     /locus_tag="VLF19_09330"
     CDS             complement(473..1510)
                     /locus_tag="VLF19_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HSE93494.1"
                     /translation="MNIFKAAAVRNPEEVTRSIPVIDFAPAFRGGADGLEAVAVQVRR
                     ASEQVGFFYLAGHGVPQPVIDDAFAASREFHAMPLEDKLALKINENNIGYLPVNASMQ
                     RASTVHRATRPNFNESFFISHDRPADHPDVVAGLPLRGRNQWPEGHASMRAAMVRYFK
                     TLEALGERMLPVLARALDLSAGFFASFFANEAHINLRFLHYPPQDTDDDEQFGQGPHT
                     DNSFFTMLAREDVPGLAVRLPSGEWVAPPVIEGTYLVNLGNVMKRWSNDRFLSTPHGV
                     LNDSGVDRYSIAFFYSPTPSATIECLPSCRSADNPARYEPAVYRDMVLDFYNANYFHR
                     QGYTGNRAAAG"
     gene            complement(1522..2010)
                     /locus_tag="VLF19_09335"
     CDS             complement(1522..2010)
                     /locus_tag="VLF19_09335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE93495.1"
                     /translation="MRLGYFAMPLHPPGSDPAQTMGDDLEQLAAMDRLGYEEAWIGGC
                     RRASSRPTGRPSPGAPGAPAVPPTGRDYFLPLLGKIRGREIIKTDPAMADADVTLAYL
                     VDHIWIVGSPATVAAKLRRLGEEVGGFGTLPVIAHEWRPREAWERSMRRLEERVLPRL
                     AA"
     gene            2146..2979
                     /locus_tag="VLF19_09340"
     CDS             2146..2979
                     /locus_tag="VLF19_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HSE93496.1"
                     /translation="MAEIVAAALTSHAPLITGKPEVSRPDRRDRLYAGFREVQRRLTA
                     ARPDLIVMFVNDHLQNFPYSNMPAFCVALADSYDAPSPGSSRLMRIPPRKVPGAAALS
                     AALLEAGLEAGIDFAYSYEIDTWDEIAVPLHFLLPEGNVPVAPIYTNCGAPPLPTPRR
                     CHEMGGFVGSFLRSRQGNERVALLATGGISHWVGTPQTGRINPEWDHWILDHIARADV
                     EPLLRLTWGEIERDGGNGGQEIRNWLALLGAVPGWKGEALAYEPVDEWITGCATVWVH
                     P"
     gene            2976..3191
                     /locus_tag="VLF19_09345"
     CDS             2976..3191
                     /locus_tag="VLF19_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93497.1"
                     /translation="MIKTSYALNKVLTAIAREHRMKDALGDEDLAGHTVTDAERAALK
                     TGDIGALYELGANPYLIRRVFRSRFRT"
     gene            3212..4141
                     /locus_tag="VLF19_09350"
     CDS             3212..4141
                     /locus_tag="VLF19_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSE93498.1"
                     /translation="MERRLPTDAEVKSWLTTRNNWGRWGKDDQRGAINLITPARRVAA
                     TRLVRSGRSVSLSRPFPKDPGMNNYYPAQHYMRILPRGTGGFSADYYGIFYHGVASTH
                     IDALCHTWDEGGMWNGRDPKKEITFDGATFGSVEHWSEGIITRGVMLDVPRHRGASHV
                     THDAPVHGWELEDIVQKRGLTLEPGDAVAVYCGRDQWQAANPDTPYGRPFGGGPNVRP
                     GLHASCLPFLRDHDVALLVWDMLDHLPIGYDIPWAVHAALFAYGVALLDNALLEPLAR
                     ACVEEGRDEFMLVIAPLRVVGGTGSPANPLAVF"
     gene            4361..5968
                     /locus_tag="VLF19_09355"
     CDS             4361..5968
                     /locus_tag="VLF19_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93499.1"
                     /translation="MAHRFAIAVLVTLSSLVLAPVPAEAQQPKRGGILRIADREAPNL
                     DPHLAVSFLTQSWANMVYSQLVRYAHGPEQKHSADFTIVPDLAEKWVYKSPTVIEFTL
                     RKGVKFHPKPPVNGREVTAEDAKYSIERFMKRSAFRARFEVVQSIDVVDRHTLRVTLK
                     EPFAPFLNHLAASTYVAILPREVEEKLTDFNRPEAMIGSGPFMLKSYQKGVQIAFERN
                     PDYYAKGLPYLDGVVIEVTPDAAARTALLRAGKVDMGHMWGYMVPEEAKSLKKTNPEM
                     VMTPTMVLGSGILYFRTDQPPFNDVRVRRAISLAIDRKAWNESLLYGEGCADAGPVPC
                     ALTDWKLPASKMDPGRAKYLVGYDPEEAKRLLAEAGFPKGFTTPMFHWPGYVPPWRTY
                     YDLAADNLAKIGVTVEFKPEEYGKYISTTYLGKYEKMAMGPQAPGNEIDDWLFSGFYP
                     EQPHNRSHVADAELNKLLVAQRRELDPAKRLAIVHDIQRYLADKAYYVYVPTAPQYIT
                     HAPPVKGFKHHDGFSLGHRLMFTWLER"
     gene            6009..7616
                     /locus_tag="VLF19_09360"
     CDS             6009..7616
                     /locus_tag="VLF19_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93500.1"
                     /translation="MRRRFVLGLLVPAVAMLVVGVPAFAQPPKQGGVLRIADREAPNL
                     DPHLNISFLTHSWANMVYSQLVRYPHGPEQKHSADFSIVGDLAEKWEYKAPTVIDFTL
                     RKGVKFHNKPPVNGREVTAEDAKYSIERFMKKSAFRARFDVVQSVDVVDRHTLRVTLK
                     EPSAPFLNHLAASTYVAILPREVEEKFKDFNRPEAMIGSGPFMLKSYQKGSQIAFERN
                     PDYYMKGMPYLDGVVIEITPDAAARLSLLRAGRVHMGHMWGYAAPEDARSLKKTNPEM
                     VMNLTGILPNGIIYFRTDQPPFNDVRVRRAISLAIDRKAWNDSILYGEACIEEGPVPC
                     AMKDWKLPVSQMDPAKAKYLVGYDPAEAKRLLADAGYPNGFTTPMYHWPGYTPPWRTY
                     YDLAADNLAKIGITVEFKPEEYGKYITTTYLGKFEKMAIGPQLPGNDVDDWLFAGFYP
                     EQPHNRSHVADAELNKMLLAQRRELDPTRRLAIVHDIQRYLADKAYYAYLPGLPQYIT
                     HAPQVKGFTYHDGFSLGYRLIFTWLDK"
     gene            7623..8570
                     /locus_tag="VLF19_09365"
     CDS             7623..8570
                     /locus_tag="VLF19_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93501.1"
                     /translation="MKQYVLRRVALAVPTLVLVSLIVFLMMRLMPGDVVVRMVEGQAY
                     APTIEALRKELGLDRPAHVQYLEWIGGILARGDFGNSYWTRQPIWDEFVQRFPVTLEL
                     ALLTILVSVVIGILVGIVSAARQDTASDYVGRVFAILALSVPYFGLAVLVVVLPSIYF
                     KWTPVWTYVPFTTNPLENLKIVIVPALVFGVTRAGPIMRIMRSSLLDILRQDYIRTAW
                     SKGLGERTIILRHALKNAMIPVVSLVGLQMPLYIGGSVIVESIFRLPGVGLFFFEALT
                     RLDYPVVQSVNLIIAGLVVGLNLVIDLSYAYLDPRIRYR"
     gene            8536..9498
                     /locus_tag="VLF19_09370"
     CDS             8536..9498
                     /locus_tag="VLF19_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93502.1"
                     /translation="MRIWTRASGTADLARAEPAPALDGARARARLAAAPPRTRPWYVD
                     VWIQMLRRKPLGTVGGVIVLVMLAAAVLADVLTPYGFAETNLRARFIASSASHWLGTD
                     QLGRDMLTRLLYGARISLYVGFGAVALGSLLATALGIFSAYFGGRVDLYLQRGVDAWM
                     AFPGLLLLMTIMSLLGPSVLNITVVLGVAFGIQNSRVVRGVALSIKEHTYIESTRALG
                     SGHARITAVHILPNVLPTIIVVATTGLSTVILTEASLSFLGLGVPPPYPTWGGMLSLA
                     GLDHMYQAPWLAIWPAVALSLAVFGFNMLGDALRDLLDPRLRGG"
     gene            complement(9440..10705)
                     /locus_tag="VLF19_09375"
     CDS             complement(9440..10705)
                     /locus_tag="VLF19_09375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE93503.1"
                     /translation="MGHSCIVRERAIVGRHELRGAVRGASRRRPESGHAGPYCAMLLA
                     QHGADVVKVEPPNGDWAREIGTRHGDQSAFSLAANLGKRSIVADLREERGRLVVHRLV
                     ERADVFLESFRPGVAARLGLGYDIVSAANPRILYVSVSGFGQTGPGRERPAVDTILQA
                     FTGLMSVNKGGDGVPHRVASVVMDMATALFTFQAVSAALYARQREPRGRYLETSLMQA
                     GAALQVVNMIASHLESGVVRPGLTPSGTFPAQDGWLNVTVLRNEDFGVLCDVLERADL
                     KADPRFADNDARFRHVGPLTEALSAAFRQRPAAEWCARLTAVGIMHTRVNAHLDYLRD
                     PHVEASGTVLWMEHPGLGKVPVPWPPRCDAAGARHPPRDGAGAGRARQGDPRRAGAGS
                     LRLRGRRTLCVNPRAAADRAGRAARRPAC"
     gene            10697..11725
                     /locus_tag="VLF19_09380"
     CDS             10697..11725
                     /locus_tag="VLF19_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HSE93504.1"
                     /translation="MPDPLNVLVTMPFDEAKLDRMRAVSPRLSVSRGDPDTADYSRAD
                     ILYAGRMPRDLVGGGARARASRLRWIQVHMAGVDSLLDHPIYTQSAVAITTSSGVHAT
                     LIAEYAITMLLALAHRVPRMIEWQTRRGWPPDEQRWPLFLPSPVRDATLGIIGYGSIG
                     RELARMARTAFAMRVLACKRDPSRRADPGYHLSGTGDPEGVLPDEWFTMARLPELLAR
                     SDVVVMSAPLTRETHHMIGARQLAAMKPSAYFINVGRGATVDEKALAEALAQRRIAGA
                     AVDVLAEEPPPAGHPLYAVANVILSPHVSGFMPTYDDECSTLFAENLRRYLAGAPLLN
                     LVDRAHGY"
     gene            11753..12595
                     /locus_tag="VLF19_09385"
     CDS             11753..12595
                     /locus_tag="VLF19_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSE93505.1"
                     /translation="MASALGSAAATIFIRQGLRSRGPYTGFWINLAVGTLGLWACVVL
                     TGGPGPVSATGLAFFVLAGLIGTVAGRLLRFVAIDQVGASISAALIALSPLISSALAI
                     LLLGEQVTVPIAAGTVVIVVGTILLSAGGRRIGVRPAQLVLPLLSATCFGVVAVLRKL
                     GLSGAGPVVGSAVNATTALIAFTAFLVASGHRDTIVWRGRGIGHFVVAGVMENAAVFL
                     TIVALSVGTVSVVLPLTGTAPIFVLLLSFFFLKGVEFLNSRVVLGTVLIVAGVYLITA
                     FPGY"
     gene            12630..13442
                     /locus_tag="VLF19_09390"
     CDS             12630..13442
                     /locus_tag="VLF19_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSE93506.1"
                     /translation="MNLLRPIDRDVVVNGLRLRLLDWGGDGRVPLLLLHGFTGHAHAW
                     DTLAIALQPRFHVYALDQRGHGDSDPADVYNAVVAFDDLSGVIDQLGLAPLVLVGLSM
                     GGRNAMYFTSRRPRDVRKLVIVDIGPEISARAAAVPAGPPEPDTWESIEHAARHLLRG
                     NPRPGIHYYRWVASTSLRQRPDGALVWKWHPSVKERRTTGDVDWWAVVRAITPPALVL
                     RGEHSPILDREVAERMAKELPQGRFVEIPDAVHTLHEDNPEAVLAALRDFLD"
     gene            13650..13946
                     /locus_tag="VLF19_09395"
     CDS             13650..13946
                     /locus_tag="VLF19_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93507.1"
                     /translation="MNAWWIIAAVPVLALLYVLIPVGLAARGRFRSHKLVRCPILGLG
                     AGVLIRRAGLAEALGWRSLRYVSDCTYWPRRRACAQRCRLAPDEEIRDFRQPVV"
BASE COUNT         2117 a         5096 c         4855 g         2265 t
ORIGIN      
        1 gcaccggaat gcacttcgag atcgtcgacg acgtcgcaca ggtcgagaca atcgcggtcg
       61 gcaggcgcat tcgggagttg tcgcggcttc agaaaatgta cggcagaggg cgatggcgca
      121 agctcaaggg tatggcacga atccggtccg cggtggagcg attcgaaagg cggagttgca
      181 ctggtatgag gcgcatgaca ttggacgaaa agaatcaagc gtaagcgtta cctcgaacta
      241 gagctgcgag aaggcaccgg cgcctgacgc cgaagtttgc ggtgtgcatc aacaacggtg
      301 agtatccggc ctcgctcgag ctgcacaaga tctatcgcgt cgtgtctgat gaagaagcta
      361 tgcgtcaggg cgacgcggct cagcgcactt tgcagcgttt cgcacgatgg catctcgcaa
      421 atcctcaaga aatttgggtc tcggtctacg aagcccgtct ccgggcgacg gctcagcccg
      481 cggccgcccg gttgccggtg tagccctgcc ggtggaagta gttggcgttg tagaagtcga
      541 gcaccatgtc gcggtacacc gccggctcgt agcgcgccgg attgtcggcc gaccggcagc
      601 tcggcaggca ctcgattgtc gccgacgggg tcgggctgta gaagaaggcg atcgaatagc
      661 ggtccacgcc ggagtcgttg agcacgccgt gcggcgtcga gaggaaccgg tcgttcgacc
      721 accgcttcat cacgttgccg aggttgacga ggtacgtccc ctcgatcacc ggcggcgcga
      781 cccactcgcc gctcggcagg cggacggcca gccccggcac gtcctcccgg gccagcatcg
      841 tgaagaacga gttgtcggta tgcggtccct ggccgaactg ctcgtcgtcg tcggtgtcct
      901 ggggcgggta gtgcaggaag cgcaggttga tgtgcgcctc gttcgcgaag aacgacgcga
      961 agaagcccgc cgacaggtcg agcgcgcggg ccagaaccgg cagcatccgc tcgccgagcg
     1021 cctcgagcgt cttgaagtag cggaccatgg ccgcccgcat cgaggcgtgg ccctcgggcc
     1081 actggttgcg cccgcgcagg ggaaggccgg cgacgacgtc ggggtggtcg gcgggccggt
     1141 cgtggctgat gaagaagctc tcgttgaagt tcggccgggt cgcccggtgc acggtcgagg
     1201 cgcgctgcat gctcgcgttc accggcagat aaccgatgtt gttctcgttg atcttgaggg
     1261 cgagcttgtc ctcgagcggc atcgcgtgga actcgcgcga ggcggcgaag gcgtcgtcga
     1321 tcaccggctg tggcacgccg tggccggcga ggtagaagaa gccgacctgc tcgctggccc
     1381 gccgcacctg gacggcgacg gcctcgagcc cgtcggcgcc gccgcgaaac gcgggagcga
     1441 agtcgatgac ggggatcgag cgggtgacct cttccgggtt ccgtaccgcc gcggccttga
     1501 agatgttcat ccgtccctcc gctacgccgc caggcgcggc agcacccgct cctcgagccg
     1561 cctcatcgag cgctcccacg cctcccgagg ccgccactcg tgggcgatca ccggcagcgt
     1621 gccgaacccg ccgacctcct cgccgagccg ccggagcttc gcggcgaccg tggcggggct
     1681 gccgacgatc cagatgtggt cgaccaggta cgcgagcgtg acgtccgcgt cggccatcgc
     1741 cgggtcggtc ttgatgatct cgcggccgcg gatcttgccg agcagcggca ggaagtagtc
     1801 ccgccctgtc ggcggcacgg ccggcgcgcc cggcgccccg ggcgacggcc tcccggtggg
     1861 tcttgaggat gcgcggcggc acccgccgat ccacgcctcc tcgtagccga ggcggtccat
     1921 ggcggcgagc tgctcgaggt cgtcgcccat cgtctgggcg gggtccgatc cgggcgggtg
     1981 caggggcatc gcgaagtagc cgaggcgcat ggcgcggatt gtactccacg agctgcctgc
     2041 gctggcttcg tcatggacgg cgggcgcccg cggtcgatct cgaggggctc gtgcgctgcg
     2101 gggcgcgccc gcctgggcta gaatcggcat tcaggagggc cgtccatggc ggagatcgtc
     2161 gcagccgcgc tcacgtccca cgcgcccctg atcaccggca agcccgaggt gtcgcggccg
     2221 gaccggcgcg accgcctgta cgccggtttc cgcgaggtgc agcgacggct gacggcggcg
     2281 cggccggacc tgatcgtgat gttcgtcaac gaccatctcc agaacttccc gtacagcaac
     2341 atgccggcgt tctgcgtggc cctggccgac tcctacgacg cgccgagccc gggcagctcg
     2401 cgcctcatga ggatcccgcc gcgcaaggtg ccgggcgccg ccgcgctctc ggcggcgcta
     2461 ctggaggctg gtctcgaggc agggatcgac ttcgcgtact cgtacgagat cgacacctgg
     2521 gacgagatcg ccgtgcccct gcacttcctc ctgcccgagg gtaacgtgcc cgtggcgccg
     2581 atctacacga actgcggggc gccgccgctg ccgacgccgc ggcgctgcca cgagatgggc
     2641 ggctttgtgg ggtcgttcct gcgctcgcgg caaggaaacg agcgcgtggc cctgctcgcc
     2701 accggcggca tctcgcactg ggtgggcacg ccccagaccg ggcggatcaa ccccgagtgg
     2761 gatcactgga tcctcgacca catcgcccgc gccgacgtcg aaccgctcct gcgtctgacg
     2821 tggggcgaga tcgagcgcga cggcggcaac ggtggccagg agatccggaa ctggctcgcg
     2881 ctgctcggcg ccgtccccgg ctggaagggc gaggcgctgg cctacgagcc ggtggacgag
     2941 tggatcaccg gctgcgcgac cgtgtgggtg catccatgat caagacctcg tacgcgctca
     3001 acaaggtgct gacagcgatc gcgcgggaac accggatgaa ggacgcgctc ggcgacgagg
     3061 acctcgcggg tcacacggtg accgacgccg agcgcgccgc gctgaagacg ggcgacatcg
     3121 gcgccctcta cgagctgggc gcgaacccct acctgatccg ccgcgtcttc cggagccggt
     3181 tccggacctg accgagggac gatccgcgac catggaacga cgcctgccga ccgacgccga
     3241 ggtgaagtcg tggctgacca cgcgcaacaa ctggggccgc tggggcaagg acgatcagcg
     3301 cggcgccatc aacctgatca cgcccgccag gcgcgtggcc gccacgcgcc tcgtgcggtc
     3361 cgggcgcagc gtctcgctga gccggccgtt ccccaaggac ccggggatga acaactacta
     3421 cccggcgcag cactacatgc gcatcctgcc gcgcggcacc ggcggcttct cggccgacta
     3481 ctacggcatc ttctatcacg gcgtcgcctc gacccacatc gatgcgctct gccacacgtg
     3541 ggacgagggc ggcatgtgga acgggcgtga cccgaagaag gagatcacct tcgacggcgc
     3601 caccttcggc tcggtcgagc actggagcga gggcatcatc acgcgcgggg tgatgctcga
     3661 cgtgccccgc caccgcggtg cctcgcacgt cacacacgac gcgcccgtcc acggctggga
     3721 gctcgaggac atcgtgcaga agcgcgggct cacgctcgag cccggcgacg ccgtggccgt
     3781 ctactgcggg cgtgaccagt ggcaggccgc caaccccgac acgccctacg ggcgcccgtt
     3841 cggtggcggg ccgaacgtgc gcccggggct gcacgcctcc tgcctgccgt tcctgcgcga
     3901 ccacgacgtc gccctgctcg tctgggacat gctcgaccac ctgcccatcg gctacgatat
     3961 cccctgggcg gtgcacgccg cgctgttcgc ctacggcgtg gcgctgctcg acaacgccct
     4021 gctcgagccg ctggcgcggg cgtgcgtgga ggaaggacgc gacgagttca tgctggtgat
     4081 cgcaccgctg cgcgtcgtcg ggggcaccgg ctcgcccgca aacccgctcg cggtgttcta
     4141 gctcggacgg gacgtcggcc attcgcccgc ggtcgggcgc ggtggctgcg catgatcccg
     4201 cgatgcgaca ccgctttcgt tgccggtagc gccctcccgg ggctcggggt cttgacagga
     4261 ttcaccacct gtacgtagac tcgcgccact ctcgcgtctc atcggatctc gcggaactcc
     4321 atcgacccgt cgttcgctcc gccaggagag ggaggcaggg atggctcatc ggttcgcgat
     4381 cgcggtgctc gtcacgctct cctcgctcgt cctggccccg gtccccgccg aggctcagca
     4441 gcccaagcgg ggcggcattc tccgcatcgc cgaccgggag gcgccgaacc tcgatcccca
     4501 cctcgcggtg agcttcctga cgcagtcctg ggccaacatg gtctacagcc agctcgtccg
     4561 ctacgcgcac ggccccgagc agaagcactc ggccgacttc acgatcgtgc ccgacctcgc
     4621 cgagaagtgg gtgtacaaga gcccgaccgt catcgagttc acgctgcgca agggggtcaa
     4681 gttccacccg aagcctcccg tcaacggccg cgaggtgacg gccgaggatg ccaagtactc
     4741 gatcgagcgc ttcatgaagc gatcggcctt ccgcgcgcgg ttcgaggtcg tccagtccat
     4801 cgacgtcgtc gatcgccaca ccctgcgcgt cacgctgaag gagcccttcg cgccgttcct
     4861 gaaccacctg gccgcgtcga cctacgtggc gatcctgcct cgcgaggtcg aggagaagct
     4921 cacggacttc aatcggccgg aggcgatgat cggcagcggc cccttcatgc tcaagtccta
     4981 ccagaagggt gtccagatcg ctttcgagcg caaccccgac tactacgcga agggactgcc
     5041 atacctggac ggcgtcgtga tcgaggtcac ccccgacgcc gccgcgcgga ccgcgctcct
     5101 gcgcgccggc aaggtggaca tggggcacat gtgggggtac atggtcccgg aggaggcgaa
     5161 gtccctcaag aagacgaacc ccgagatggt catgacgccc acgatggtcc tcggcagcgg
     5221 catcctctac ttccgcaccg accagccccc gttcaacgac gtgcgcgtcc gccgggccat
     5281 ctcgctcgcc atcgaccgga aggcgtggaa cgaatcgctg ctgtacggcg aggggtgcgc
     5341 cgacgccggg ccggtgccat gcgcgctgac ggactggaag ctgccggcct cgaagatgga
     5401 cccgggcagg gccaagtacc tggtggggta cgaccccgag gaggccaagc gcctgctggc
     5461 cgaggcgggc ttcccgaagg gcttcaccac gcccatgttc cactggccgg gctacgtgcc
     5521 gccgtggcgg acctactacg acctcgccgc cgacaacctg gccaagatcg gcgtgaccgt
     5581 ggagttcaag ccggaggagt acggcaagta catctccacg acctacctcg gcaagtacga
     5641 gaagatggcc atggggccgc aggccccggg caacgagatc gacgactggc tcttcagcgg
     5701 cttctatccg gagcagcccc acaaccgcag ccacgtcgcc gacgccgagc tgaacaagct
     5761 gctggtggcg cagcggcgtg agctcgaccc cgcgaagcgg ctggccatcg ttcacgacat
     5821 ccagcgctat ctcgccgaca aggcgtacta cgtctacgtg ccgacggcgc cccagtacat
     5881 cacgcacgcg ccgccggtga agggcttcaa gcaccacgac ggcttcagcc tgggccatcg
     5941 cctcatgttc acctggctcg agcgttagcc ggggcgcgac accacccgct cgacgaggag
     6001 gatccagcat gcgtcgacgt ttcgtgctcg ggctgttggt tcccgcagtg gcgatgctgg
     6061 tcgtcggagt tcccgccttc gcccagccgc ccaagcaggg cggcgtgctc cgcatcgccg
     6121 atcgcgaggc gccgaatctc gacccgcacc tcaacatcag cttcctcact cactcctggg
     6181 ccaacatggt ctacagccag ctcgtgcgct acccgcacgg cccggagcag aagcactcgg
     6241 ccgacttctc gatcgtggga gacctcgccg agaagtggga gtacaaggcc cccaccgtga
     6301 tcgacttcac gctccggaaa ggcgtcaagt tccacaacaa gcctcccgtc aacggccgcg
     6361 aggtgacggc ggaggacgcc aagtactcga tcgagcggtt catgaagaaa tcggcgttcc
     6421 gggcgcgctt cgacgtcgtc cagtcggtcg acgtcgtgga ccgccacacc ctgcgcgtca
     6481 cgctcaagga gccgtctgcg ccgttcctga accacctggc cgcctcgacc tacgtggcga
     6541 tcctgccccg tgaggtcgag gagaagttca aggacttcaa ccggccggaa gcgatgatcg
     6601 gctccggccc gttcatgctg aagagctacc agaagggctc gcagatcgcc ttcgagcgca
     6661 accccgacta ctacatgaag gggatgccgt acctcgacgg cgtcgtgatc gagatcaccc
     6721 ccgacgcggc cgcgcggctt tccctcctcc gcgcgggcag ggtccacatg gggcacatgt
     6781 gggggtacgc ggccccggag gacgccaggt ccctcaagaa gaccaacccc gagatggtca
     6841 tgaacctcac ggggatcctc cccaacggga tcatctactt ccggacggac cagccaccgt
     6901 tcaacgacgt gcgcgtgcgg cgagctatct cgctggccat cgatcggaag gcgtggaacg
     6961 actcgatcct ctacggggag gcgtgcatcg aggagggccc ggtgccctgt gccatgaagg
     7021 actggaagct cccggtctcc cagatggatc ccgcgaaggc caagtacctc gtcggatacg
     7081 acccggccga ggcgaagcgg ctcctggccg acgcgggcta cccgaacggc ttcaccacgc
     7141 ccatgtacca ctggccgggg tacacgccgc cctggcggac gtactacgac ctcgccgccg
     7201 acaacctcgc gaagatcggg atcaccgtcg agttcaagcc cgaggagtac ggcaagtaca
     7261 tcaccacgac ctacctcggc aagttcgaga agatggcgat cggccctcag ctccccggca
     7321 acgacgtcga cgactggctc ttcgccggct tctatcccga gcagccgcac aaccgcagcc
     7381 acgtcgccga cgccgagctg aacaagatgc tgctcgccca gcggcgcgag ctcgacccca
     7441 cgaggcgcct agccatcgtc cacgacatcc agcgctacct ggcagacaag gcctactacg
     7501 cgtacctgcc gggcttgccc cagtacatca cacacgcgcc ccaggtgaag ggcttcacgt
     7561 accacgacgg gttctcgctg ggctaccggc tcatcttcac ctggctcgac aagtagggcg
     7621 ccgtgaaaca gtacgtcctg cgccgggtgg ccctcgccgt tccgacgctc gtgctggtga
     7681 gcctcatcgt gtttctcatg atgcggctca tgcccggcga cgtggtggtg cgaatggtgg
     7741 agggccaggc ctacgcgccg accatcgagg cgcttcgcaa ggagctcggg ctcgaccgcc
     7801 ccgcccatgt gcagtacctc gagtggatcg gcggcatctt ggcccggggc gacttcggga
     7861 attcctactg gacacgccag ccgatctggg acgagttcgt gcagcgcttc ccggtgacgc
     7921 tcgagctggc cctgctgacc atcctcgtct cggtggtcat cggcatcctc gtgggcatcg
     7981 tctccgcggc gcggcaggac acggcctcgg actacgtcgg ccgggtcttc gccatcttgg
     8041 ccctctccgt cccgtacttc ggcctcgccg tgctcgtggt ggtgctgccg tcgatctact
     8101 tcaagtggac gccggtgtgg acctacgtgc ccttcacgac gaacccgctc gaaaacctca
     8161 agatcgtcat cgtccccgcc ctggtcttcg gcgtcacccg cgccgggccc atcatgcgga
     8221 tcatgcgctc ctcgctgctg gacatcctgc ggcaggacta catccgcacc gcgtggtcca
     8281 aggggctcgg cgagcggacc atcatcctcc gccacgcgct caagaacgcc atgatccccg
     8341 tcgtcagcct ggtgggcctg cagatgccgc tctacatcgg cgggtcggtc atcgtcgagt
     8401 cgatcttccg gctgcccggc gtcggcctct tcttcttcga ggccctcacg cgcctggact
     8461 acccggtggt gcagtccgtg aacctcatca tcgcgggcct ggtggtgggc ctcaacctcg
     8521 tcatcgacct ctcctatgcg tatctggacc cgcgcatccg gtaccgctga tctcgcgcgg
     8581 gccgagcccg cgcccgcgct cgacggggcc cgcgcgcggg ccaggctcgc ggcggcgccg
     8641 ccgcggacgc gtccctggta cgtggacgtc tggatccaga tgctccgccg caagccgctc
     8701 ggcaccgtcg gcggcgtcat cgtgctcgtc atgctcgccg ccgcggtgct ggccgacgtc
     8761 ctcaccccct acggcttcgc cgagacgaac ctgcgcgcgc gcttcatcgc ctccagcgcc
     8821 tcccactggc tcggcaccga ccagctcggg cgcgacatgc tgacgcggct cctctacggc
     8881 gcgcggatct ccctctacgt gggcttcggc gccgtcgcgc tgggcagctt gctggccacg
     8941 gcgctgggga tcttctcggc ctacttcggc ggccgcgtgg acctgtacct gcagcgtggg
     9001 gtggacgcct ggatggcctt ccccgggctg ctcctgctga tgacgatcat gtccctgctc
     9061 gggccgagcg tgctcaacat caccgtggtg ctcggcgtcg ccttcggcat ccagaactcc
     9121 cgggtcgtcc gcggcgtcgc cctgtcgatc aaggagcaca cctacatcga gagcacccgc
     9181 gcgctgggct cgggccacgc gcgcatcacc gccgtccaca tcctgcccaa cgtgctgccg
     9241 accatcatcg tggtcgccac caccggcctc tccaccgtga tcctcaccga ggccagcctc
     9301 tcgttcctcg gcctgggcgt gccgccgccc tacccgacgt ggggcggcat gctgtcgctg
     9361 gcggggctcg accacatgta ccaggcaccg tggctcgcca tctggccggc cgtcgcgctg
     9421 tcgctcgccg tgttcggatt caacatgctg ggcgacgcgc tgcgcgacct gctcgatccg
     9481 cggctgcgcg ggggttgacg cagagcgttc ttctgccccg gagcctcaac gatccagccc
     9541 cagctcggcg cggatctcct tgccgtgctc gcccagcgcc ggcgccatcg cgcggcgggt
     9601 gccgggcgcc ggcggcatca caccgtgggg gccatggcac cggcaccttg cccagccccg
     9661 gatgctccat ccacagcacg gtaccgctgg cttccacgtg cgggtcgcga aggtagtcga
     9721 ggtgcgcgtt gacccgggtg tgcatgatgc ccacggcggt caggcgtgcg caccattcgg
     9781 cggccgggcg ctggcggaat gccgcgctga gggcctcggt cagcggcccg acgtggcgga
     9841 agcgcgcgtc gttgtcggcg aagcgcggat cggccttgag gtcggcgcgc tccaggacgt
     9901 cgcacagcac gccgaaatcc tcgttgcgca gcacggtgac gttgagccag ccgtcctgcg
     9961 cggggaacgt gcctgacggc gtgaggccgg gccggaccac gccgctctcg aggtgactgg
    10021 cgatcatgtt gacgacctgc agcgcggcgc cggcctgcat gaggctcgtc tcgaggtagc
    10081 gcccgcgcgg ctcgcgctgg cgcgcgtaga gcgccgccga cacggcctgg aaggtgaaga
    10141 gggccgtggc catgtccatc acgacggagg ccacgcggtg gggcacgccg tcgccgccct
    10201 tgttgaccga catgagcccg gtgaaggcct gcaggatggt gtccacggcc gggcgctcgc
    10261 gccccggccc ggtctggccg aagccggaca cggagacgta gaggattctc ggattggcgg
    10321 cggagacgat gtcgtagccg aggccgagcc gggcggccac gccggggcga aaactctcga
    10381 ggaagacgtc ggcgcgctcg accaggcggt gcacgacgag ccggccgcgc tcttcgcgga
    10441 ggtcggcgac gatgctgcgc ttgccgaggt tggcggccag cgagaaggcc gactggtcgc
    10501 cgtggcgcgt gccgatctcg cgcgcccagt cgccgttcgg cggctcgacc ttcacgacgt
    10561 cggcgccgtg ctgggccagg agcatcgcgc agtaggggcc ggcgtgccct gactcaggtc
    10621 ggcgacgcga agcccctcga acggcgcctc gtagctcatg gcggccgacg atagcacgct
    10681 ctcgtacaat gcaggaatgc ccgatcccct gaacgtgctg gtgacgatgc ccttcgacga
    10741 ggcgaagctc gaccggatgc gcgcggtgtc gccgcggctg tccgtgagcc gtggcgaccc
    10801 ggacaccgcc gactactccc gcgcggacat cctctacgcg ggcaggatgc cgcgcgatct
    10861 cgtgggcggc ggggcgcggg cccgggcgtc ccggctccgg tggatccagg tgcacatggc
    10921 cggggtggac tcgctgctcg accaccccat ctacacgcag agcgccgtcg ccatcaccac
    10981 gtcgagcggc gtgcacgcca cgctgatcgc cgagtacgcg atcacgatgc tgctggcgct
    11041 cgcgcaccgc gtgccgcgca tgatcgagtg gcagacccgg cgcggctggc cgcccgacga
    11101 gcagcgctgg ccgctgttcc tgccgagccc ggtgcgtgac gctacgctcg ggatcatcgg
    11161 ctacggcagc atcgggcgcg agctggcccg catggccagg accgcgttcg cgatgcgggt
    11221 gctcgcgtgc aagcgcgacc cgtcgcgccg ggccgacccc ggctaccacc tgtccggcac
    11281 gggggacccc gagggcgtcc tgcccgacga gtggttcacg atggcgcggc tgccggagct
    11341 gctcgcgcgg tcggacgtgg tggtgatgag cgcgccgctc acgcgcgaga cgcatcacat
    11401 gatcggcgcc cggcagctcg ccgcgatgaa gccctcggcc tacttcatca acgtcggccg
    11461 gggcgccacc gtggacgaga aggcgctcgc cgaggcgctg gcgcagcggc gtatcgccgg
    11521 ggccgcggtg gacgtgctcg ccgaggagcc gccgcccgcg gggcatccgc tctacgcggt
    11581 ggccaacgtg atcctctcgc cgcacgtgtc gggcttcatg cccacctacg acgacgagtg
    11641 ctcgaccctg ttcgccgaga acctgcgccg gtacctcgcc ggcgcgccgc tcctgaacct
    11701 ggtcgatcgc gcgcacggat actaggccat ccgcacggtc cacgtcctgg cgctggcgag
    11761 cgcgctcggc tcggcggcgg cgacgatctt catccggcag ggcctgcgga gccgcggccc
    11821 ctacaccggc ttctggatca acctcgccgt cggcacgctc ggcctctggg cgtgcgtggt
    11881 cctcaccggc ggacccgggc ccgtgtcggc gaccgggctc gccttcttcg tcctggccgg
    11941 cctcatcggc accgtggccg gtcggctcct ccggttcgtc gccatcgacc aggtcggcgc
    12001 ctcgatctcg gcggcgctca tcgccctcag cccgctgatc tcctcggcgc tggccatcct
    12061 cctgctcggc gagcaggtca ccgtcccgat cgccgccggc accgtcgtca tcgtggtggg
    12121 caccatcctg ctgtccgccg gaggccgccg catcggcgtg cggcccgcgc agctcgttct
    12181 tccgttgctg tcggccacct gcttcggcgt cgtcgccgtc ctcaggaagc tcggcctgag
    12241 cggcgcagga ccggtggtgg gctcggcggt gaatgccacg accgccctca ttgccttcac
    12301 ggcgttcctc gtggcctcgg gccaccgcga cacgatcgtc tggcggggtc gcgggatcgg
    12361 gcacttcgtg gtcgcgggcg tcatggagaa cgcggcggtc ttcctgacca tcgtggccct
    12421 cagcgtgggc acggtgagcg tggtactgcc tctcacgggg acggcgccca tcttcgtgct
    12481 cctgctctcc ttcttcttcc tcaagggcgt ggaattcctc aacagccggg tggtcctggg
    12541 caccgtcctc atcgtcgccg gggtctacct catcaccgcg tttccgggct actgaccggc
    12601 ggccgccccg cctggtatgc tctggcgcca tgaacctcct gcgcccgatc gaccgcgacg
    12661 tcgtcgtcaa cggcctgcgc ctgcgcctgc tcgactgggg cggcgacggc cgggtgccgc
    12721 tgctgctcct gcacgggttc acgggccacg cccacgcctg ggatacgctg gccatcgcgc
    12781 tccagcctcg cttccacgtg tacgcgctcg accagcgggg acacggcgac agcgatcccg
    12841 ccgacgtcta caacgcggtg gtcgccttcg acgacctctc gggcgtgatc gaccagctcg
    12901 gtctggcccc gctggtgctc gtcggcctct cgatgggcgg ccgcaacgcg atgtacttca
    12961 cgtcgcggcg cccccgcgac gtccggaagc tggtgatcgt agacatcggc cccgagatca
    13021 gcgcgcgcgc ggccgcggtg ccagccgggc cgcccgagcc cgacacctgg gagagcatcg
    13081 agcacgccgc ccggcacctg cttcgcggga acccgcgccc gggcattcac tactatcgct
    13141 gggtcgcctc gacgagcctg cggcagcgcc cggacggggc gctcgtgtgg aagtggcatc
    13201 cgagcgtcaa ggagcgccgc accacggggg atgtcgactg gtgggccgtg gtgcgtgcga
    13261 tcacgccgcc ggcgctggtc ctgcgcgggg agcacagtcc gatcctggac cgcgaggtcg
    13321 ccgagcggat ggcgaaggaa ctgccgcagg gccggttcgt cgagatcccc gacgcggtgc
    13381 acacgctcca cgaggacaac cccgaagccg tcctggccgc gctgagagat ttcctcgatt
    13441 gatgacttgg gggccccggg catggccccc aagcccccag gaacccggca gagggcccag
    13501 tgtgtgctct cctctcatcg caccacgtaa ttgacccaga tgcctcattg aatcccgccc
    13561 tgccgaggcg tactcggtgg gccatcaggc gacggcgcac cgcccggccg ggggcccggg
    13621 aggcggcgac gtcggggaag aggtgcgtga tgaatgcctg gtggatcatc gccgctgtcc
    13681 ctgtcctggc cctcctgtac gtcctgatcc cggtcggtct cgccgcgcgt ggacgcttcc
    13741 gcagtcacaa gctcgtccgg tgcccgatcc tcggtctggg cgccggcgtg ctcatccgcc
    13801 gcgccgggct ggccgaggcc ctcggctggc ggtcattgcg ttatgtgtcc gactgcacct
    13861 attggcctcg gcgcagggcg tgtgcgcagc gctgccggct cgctcctgac gaggagattc
    13921 gcgactttcg ccagccggtg gtgtgaggct gacgtcgagc ggacggggcg gcgcggaggc
    13981 ggattgcctc gcgcgttgct gaacgccccg tcgtgctcga gcctgggagc tcgagctggt
    14041 gcccctccgc gccggcgggt tgatcccgat cgcgcgtcag tgccacgggc gcgcgcgagt
    14101 tcgtcgaccg atgaggtcct tcattcgcgc gccctcgggg ctgttctgtc catcgcggtc
    14161 gggttgcgca cgttggccgg cgccgaggcg cggcaaccgc tcgggatcga agcctcgttc
    14221 tccaacgttc agccgcccag gttgtagagc cgcgcgcagt tgtcccagag gatcttccgc
    14281 ttgctctcct cgctgacgcc gggcagcccg aggaacgtgt cgatggcgtt cgg
//
LOCUS       DATIAZ010000262         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1122373, whole genome shotgun sequence.
ACCESSION   DATIAZ010000262 DATIAZ010000000
VERSION     DATIAZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122373"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VLF19_09400"
     CDS             complement(<1..629)
                     /locus_tag="VLF19_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE93508.1"
                     /translation="MLAEGMATTVFTNAFLIDCTGRDPMAGAAVVVEDDRIKDVLPSG
                     KVGPLPGRVTTLDLEGATLMPGLTDAHVHICAVTENITDQHRHYPPSYIAARAMRRAE
                     ECLLQGFTTVRDAGGADYGFRQVLEEGHFPGPRLLVSGRYISQTGGHGDKRRRAEWTD
                     PIACCVGMIGAIADGPDEVRRAARENIRRDVDQIKIMASGGAMSPSDEL"
     gene            742..2700
                     /locus_tag="VLF19_09405"
     CDS             742..2700
                     /locus_tag="VLF19_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSE93509.1"
                     /translation="MATDHVFFGDLALVFVAAVLGGALARLARQPLILGYVLAGIAVS
                     PFTPGPIVASVRTFELFAEIGVVLLMFSIGIEFSLRDLAGVKWAALIGAPVGIVLHVA
                     LGLAVGWALGWPPAQGAVIGVVTSVASTMVLVRLLMDRGELHARHGRILLGTVIVEDL
                     AVVVLIMLISAMGPLEGSRVVAVAVAAGKALVVLVPFLWLAWRVVPHLMARAARTQSA
                     DLFLLVALAVGVGTAALTQAVGLSLALGAFLAGLLVNASDYAHETLDRLLPLRDVFVA
                     FFFVTIGAVIDPRVLADNVPLLTAVVALVVAGKFLVRTALTRAFGFALPTAALVGVGL
                     AQIGEFSFVMVQGARSAGLVGADVYNAVLAASLLSILLNALLMRATSAVAALEPVRVP
                     HPVRGGLYGWDHLHGHVVLCGFGRVGSAVSEALETFRLTYVVIEIDPQVAQALRARGI
                     ACLLGDAARTRLLEAAGVARASLIVVAVPEDARALLAVRRIRALNPSATILARAHQRE
                     SSDALRRAGATEVIQPELEAGLTLIRHGLGALALPKESLIAYLECLRDAIGGGPERAL
                     AARQGLPRVREITLAADGFVDQPLREARLLERFGLTVVRIERADGDVVMTPDGETLLR
                     AGDRIRVFGLPEQIDAFLAASHVEHDSA"
     gene            complement(2778..3509)
                     /locus_tag="VLF19_09410"
     CDS             complement(2778..3509)
                     /locus_tag="VLF19_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HSE93510.1"
                     /translation="MEALLTTDGLIALVTLAAMEIVLGIDNLVFIAILTGKLPAKQQP
                     LARRLGLALALLTRIALLFAITWMMSLTAPLVTVLGHSVSGRDLILIAGGLFLMFKAT
                     WEIYDKLELRDGHAPTAAGGRRSMALVVAQIMVIDIVFSLDSVITAVGMVDELAIMVT
                     AVVIAIGVMLVSMEPVAGFVERHPSIKILALAFLLLIGVMLVAEGMGRHVEKGYIYFA
                     MAFSLFVELLNMRFRRRQRPARSRG"
BASE COUNT          455 a         1275 c         1303 g          569 t
ORIGIN      
        1 tccagctcgt ccgagggcga catggcgccg cccgaggcca tgatcttgat ctggtccacg
       61 tcgcgccgga tgttctcgcg cgccgcccgg cgcacctcgt cgggcccgtc ggcgatggcg
      121 ccgatcatgc ccacacagca ggcgatcggg tcggtccact ccgcccggcg tcgcttgtcg
      181 ccgtggccac cggtctggga gatgtagcgg ccgctcacga gcagccgcgg ccccgggaag
      241 tggccctcct cgagcacctg gcggaagccg tagtcggcgc ctccggcgtc gcgcacggtc
      301 gtgaagccct gcagcaggca ctcctcggcg cggcgcatcg cgcgggcggc gatgtacgac
      361 ggagggtagt ggcggtgctg gtccgtgatg ttctcggtca ccgcgcagat gtgcacgtga
      421 gcgtcggtca gccccggcat cagcgtcgcg ccctcgaggt cgagcgtggt cacgcggccg
      481 ggcagcgggc cgaccttgcc gctcgggagc acgtccttga tgcggtcgtc ctcgacgacg
      541 acggccgcgc ccgccatggg atcgcggccg gtgcaatcga tcaggaacgc gttggtgaag
      601 acggtggtgg ccatgccttc ggcgagcata cacgacccat ccggccgccg aaaggctgag
      661 gatggtctcg cccaggccca gcccgacgtt gcgttgcgcc gcgaggtcca ggcgttgggg
      721 taaagttcta ccacctacgc catggccacc gaccacgtct tcttcggcga tctcgcgctg
      781 gtgttcgtgg ccgccgtgct gggcggcgcc ctggcgcgcc tggcgcggca gccgctgatc
      841 ctcggctacg tgctggccgg catcgcggtg agcccgttca cgccgggtcc catcgtggcc
      901 agcgtgcgga cgttcgagct gttcgcggag atcggcgtgg tcctcctgat gttctccatc
      961 ggcatcgagt tctcgctgcg cgacctcgcc ggcgtgaagt gggccgcgct gatcggcgcg
     1021 ccggtcggga tcgtgctcca cgtggcgctg gggctcgccg tcggctgggc gctcgggtgg
     1081 ccgcccgccc agggcgccgt catcggcgtc gtcacgtcgg tggcgagcac gatggtgctc
     1141 gtacgcctgt tgatggaccg cggcgagctg cacgcgcgtc acggccgcat cctgctcggg
     1201 accgtcatcg tcgaggacct ggccgtcgtg gtgctgatca tgctcatctc ggcgatgggg
     1261 ccgctggagg ggagccgggt cgtggccgtc gcggtggcgg ccggcaaggc gctggtcgtc
     1321 ctggtgccct tcctgtggct ggcctggcgc gtggtccctc acctcatggc gcgggcggcg
     1381 cgcacgcaga gcgccgacct gttcctgctg gtcgcgctgg ccgtcggcgt cggcaccgca
     1441 gcgctgaccc aggcggtggg gctctcgctg gcgctcggag cgttcctggc cggcctgctc
     1501 gtcaacgcgt cggactatgc gcacgagacg ctcgaccggc tgctgccgct ccgggacgtc
     1561 ttcgtggcct tcttcttcgt gaccatcggc gccgtcatcg atccgcgcgt gctcgccgac
     1621 aacgtgccgc tgctgaccgc cgtcgtcgcg ctggtggtcg cggggaagtt cctggtccgg
     1681 acggccctca cccgggcctt cggcttcgcg ctgccgacgg ctgcgctcgt cggcgtgggc
     1741 ctggcgcaga tcggcgagtt ctcgttcgtg atggtgcagg gggcgcgcag cgcaggcctc
     1801 gtcggcgccg acgtctacaa cgccgtgctg gccgcctcgc tgctgtcgat cctgctcaac
     1861 gcgctgctga tgcgggcgac ctcggcggtc gccgcgctcg agcccgtgcg cgtgccccac
     1921 ccggtgcgag gcgggctcta cggctgggac cacctgcacg gccacgtggt gctgtgtggg
     1981 ttcggccgcg tcggcagcgc cgtcagcgag gcgctggaga cgtttcgcct gacctacgtg
     2041 gtcatcgaga tcgatccgca ggtggcgcag gcactgcgcg cgcgggggat cgcgtgcctg
     2101 ctcggcgacg ccgcacgcac gcgcctgctg gaggccgcgg gtgtggcgcg cgcctccctg
     2161 atcgtggtcg cggtgcccga ggatgcgcgg gcgctgctcg ccgtgcggcg tatccgcgcg
     2221 ctgaacccct cggcgacgat cctcgcgcgc gcgcaccagc gggagtcgag cgacgcgctc
     2281 cgtcgcgccg gcgccaccga ggtgatccag cccgagctgg aagcggggct cacgctgatc
     2341 cgtcacggcc tcggcgcgct ggcgctgccc aaggaaagcc tcatcgcgta tctggagtgt
     2401 cttcgcgacg ccatcggcgg cgggccggag cgcgcgctcg cggcccgcca gggcctgccg
     2461 cgggtgcgcg agatcacgct cgccgccgac ggcttcgtcg atcagcccct gcgcgaggcg
     2521 cggctgctgg agcgtttcgg tctgaccgtg gtacggatcg agcgggccga tggcgacgtc
     2581 gtcatgactc cggacggcga gacgctgctg cgcgcaggcg accgcatacg cgtcttcggg
     2641 ctgcccgagc agatcgacgc ctttctcgcc gcgtcgcacg tggagcacga ctccgcctag
     2701 cccacccgag ggccggggga gccggcccac tccgcccgac gctgcgcgag gcgacgacgg
     2761 acgtccgacg cgggaagcta accgcggctc cgcgcgggcc tctgccgccg gcggaaccgc
     2821 atgttcagca gctcgacgaa gagcgagaat gccatcgcga agtagatgta ccccttctcg
     2881 acgtgccggc ccatgccctc ggcgacgagc atcaccccga tcaggaggag gaaggcgagc
     2941 gccaggatct tgatgctcgg gtggcgctcc acgaagccgg cgacgggctc catcgatacc
     3001 agcatcacgc cgatggcgat caccacggcc gtcaccatga tggcgagctc gtcgaccatg
     3061 cccacggccg tgatgacgga gtcgagcgag aacacgatgt cgatcaccat gatctgcgcg
     3121 accaccaggg ccatggagcg ccggccgccg gcggcggtgg gcgcgtggcc gtccctcagc
     3181 tcgagcttgt cgtagatctc ccaggtggcc ttgaacatga ggaacaggcc gccggcgatc
     3241 aggatcaggt cccggccgga cacgctgtgc ccgagcaccg tcaccagggg cgcggtgagg
     3301 ctcatcatcc acgtgatcgc gaagagcagc gcgatacgag tgagcagggc cagcgccagc
     3361 cccaggcgcc gcgccagcgg ctgctgcttc gccggcagct tgccggtgag gatggcgatg
     3421 aagacgaggt tgtcgatgcc gaggacgatc tccatcgccg ccagcgtcac cagcgcgatg
     3481 agtccgtccg tcgtcagcag cgcttccatg ctccactcct ctcagcgtgc cagccgcgcc
     3541 tccacgccct gccacatcag ctccgcccag cgcgcgtacc ccgcgtcgga ggggtggtaa
     3601 cc
//
LOCUS       DATIAZ010000263         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1649879, whole genome shotgun sequence.
ACCESSION   DATIAZ010000263 DATIAZ010000000
VERSION     DATIAZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649879"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1442
                     /locus_tag="VLF19_09415"
     CDS             126..1442
                     /locus_tag="VLF19_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSE93511.1"
                     /translation="MTATTQRPVPTIETFAADVGGCLGYLVVDEASRTAMAIDPRLDH
                     VERFQDALAARDLRLAWVLDTHTHADHLSGVRTLAQRTGAVVLAHTSSKFRNPARRVA
                     GGSTFALGTTTVTVLNAPGHTPDSLAVLVAGHLFTGDALFAGGAGRTDFMGGSASDLF
                     DTLRTFEALPDDTVVHPGHDYVGRPLTTIGEEKAGNPLLRERDRAAFVSRLSSRAAPP
                     ANMAAILRHNLGEVDAATVAPAELEALRRQGLAPFLLDVRSPLEFEGERIESARLIPL
                     EQLEARLDEVPDGTDVVVVCRTGIRATIAAEMLAHGGRRVRVLEGGMRAWRRAGLPIR
                     QGRRRLPVDRQVQLIAGGMVLTGVTLGSVFSPWFLGIAAFFGAGLVFAGATGTCGLAQ
                     VLMRMPWNRPLDVPAPGAAATCAATDPGPASCAAPATCAAPGEGRR"
     gene            1460..1789
                     /locus_tag="VLF19_09420"
     CDS             1460..1789
                     /locus_tag="VLF19_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSE93512.1"
                     /translation="MRARLPREALEIVAQRFRVLGDATRLALLQALFDGERSVQELCA
                     HVGVSQANASKHLALLLEQGLVARRRDGYFTRYRIADAGLEDLCERVCTALARRHAAL
                     GAHLVPG"
     gene            1864..2175
                     /locus_tag="VLF19_09425"
     CDS             1864..2175
                     /locus_tag="VLF19_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93513.1"
                     /translation="MKLPRLTLIVAVYVALDVSNPLMPGALTFGVEDSVEARQADRFR
                     GHDDAASPALTPAVEGLAPVEPSVTASRLVVVTGLRFRRLHAARSDPSQRAPAPSPED
                     H"
     gene            2317..3378
                     /gene="hflK"
                     /locus_tag="VLF19_09430"
     CDS             2317..3378
                     /gene="hflK"
                     /locus_tag="VLF19_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HSE93514.1"
                     /translation="MSSWPSREGMPDLSEQFEPLRRAVQRFGRQRLVTVVVAGAVLLW
                     LASGFYVVGPGERGVVLLFGRAVDQTESGLRYRLPSPIQSHQIVDVATVRRAEVGFRS
                     DRGGARALPSESLMLTGDENIVDVQLFVQYLVRDPAKFLFRARHPEMALHSSAEVALR
                     AAVGETNIDYTMTEGRVEVQDRVKKQLQELLDLYGTGLLVTEARLLVVDPPREVKEAF
                     HDVVRAWEDRERLIKEAEGYAEGVVPKARGEAAQMLRTAEAYREQRLIRARGDADRFV
                     RVHNEYRKAEAVTRDRLYLEMVDRVLPRTKTVVVDSSGDRSSVVPLLPLGTFLPGPPT
                     AKTNSREPASAPAATTGGR"
     gene            3381..>4186
                     /locus_tag="VLF19_09435"
     CDS             3381..>4186
                     /locus_tag="VLF19_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HSE93515.1"
                     /translation="MAWRIAVIVLITAVLGGATYIVPEWQQAIVTQFGQPIRTVREPG
                     LYWKVPFLHTVTYFDKRVLAADTGGAEYITLDKKRLLIDHISRWEIVDPLQFYRTVRD
                     ERGAMARLDDVITARLRQEVAKETFKEIIREKREGIMETVTAEARALATPFGIRVLDV
                     RVKRADLPAEVQASVFARMQAERQRIALRYRAEGEEKGREIRANADKEREIILAKAYQ
                     ESQGLRGAGDAEATAVTGQAFGRDPAFYGFMRRLETYERVFTPETTIVLRP"
BASE COUNT          595 a         1485 c         1513 g          593 t
ORIGIN      
        1 ctcggcggtg gccgccgtcg gccccgcacg ccatgcccgc cattcggccc gcgccggcac
       61 cgccgcttga ctagatgact acttagttat atattctgcc cggggggaaa aggaacatga
      121 cgacgatgac cgcgaccacc cagaggccgg tgccgacgat cgagaccttc gccgcggacg
      181 tcggcgggtg cctgggctac ctcgtggtgg acgaggccag ccgcaccgcc atggcaatcg
      241 acccgcgtct ggaccacgtc gagcgcttcc aggacgcgct ggcggcccgt gacctccggc
      301 tggcgtgggt gctcgacacc cacacacacg ccgaccatct ttccggggtg cgcacgctcg
      361 cacagcggac gggcgccgtt gtgctggccc atacgtcgtc gaagttcagg aatccggcgc
      421 ggcgtgtcgc gggtggatcg acgttcgcac tgggcacgac gaccgtgacc gttctgaacg
      481 ctccggggca cacccccgac tcgctggccg tgctcgtcgc cggccacctg ttcacgggcg
      541 acgccctgtt cgccggcgga gccggccgga ccgacttcat gggcgggagc gcgtcggacc
      601 tcttcgacac gctgcggacc ttcgaggcgc ttcccgacga cacggtcgtg caccccgggc
      661 acgactacgt cggccgcccg ctgaccacga tcggcgagga gaaggccggc aacccgctcc
      721 ttcgggagcg cgaccgggcc gccttcgtct cccggctgtc ttccagggcc gcgccgccgg
      781 cgaacatggc cgcgatcctg cgccacaacc tcggtgaggt cgacgcggcc acggtcgcgc
      841 cggccgagct cgaggcgctg cgccggcagg gcctggcgcc gttcctgctc gacgtgcgca
      901 gcccgctcga gttcgaggga gagcgcatcg agagcgcccg gctgatcccg ctggaacagc
      961 tggaggcacg gctggacgag gtacccgacg ggaccgacgt cgtcgtcgtg tgccgtacgg
     1021 gcatacgcgc caccatcgcc gccgagatgc tggcccatgg cgggcgacgt gtgcgggttc
     1081 tcgaaggcgg catgcgagcc tggcgccggg cgggactgcc gatccggcag gggcgacgtc
     1141 gtctgcccgt cgatcgccag gtgcagctga tcgccggggg catggtactc accggcgtga
     1201 cgctgggcag tgtgttcagt ccctggttcc tcggcatcgc cgccttcttc ggggcaggtc
     1261 tcgtcttcgc gggcgccacg ggcacctgtg gcctggcgca ggtcctcatg cggatgccgt
     1321 ggaaccgacc gctcgatgtg ccggcgcccg gtgccgccgc cacgtgcgcc gcgaccgatc
     1381 ccggcccggc gtcctgcgcg gcgcccgcga cgtgcgcggc gccgggggag ggccggcgct
     1441 gagcgcggca ggcaagcgaa tgagggcgag actgcctcgt gaagcgctgg agatcgtcgc
     1501 ccagcggttc cgcgtcctcg gcgacgccac gcggctggcg ctgctgcagg ccctcttcga
     1561 cggcgagcgc agtgtccagg agctctgcgc gcacgtaggt gtctcgcagg cgaatgccag
     1621 caagcacctc gcgctcctgc tcgagcaggg gctggtcgcg cgccgccgtg acggctactt
     1681 cacgcgctac cgcatcgccg acgcagggct cgaggatctc tgcgagcgcg tctgcaccgc
     1741 gctcgcccgc cgccacgccg ccctgggcgc tcacctcgtt cccgggtgac gtcgattcct
     1801 gctcgggttg cccgactccc ggcccgatgg gatcagacgc ccgccgggcg tagaatggcg
     1861 tgaatgaagc tgccgaggct gacgctcatc gtggcggtct acgtggcgct cgacgtctcc
     1921 aacccgttga tgccgggcgc gctgacgttc ggcgtcgagg actccgtcga ggcccggcag
     1981 gcggaccgct ttcggggcca cgacgatgcg gcctcgcccg cgctcacgcc ggcggtcgag
     2041 ggcctcgctc cggtcgagcc cagcgtcacg gcgagccgcc tggtcgtcgt gacagggttg
     2101 cgcttccggc ggcttcacgc ggcccgctcc gatccatcgc agcgggcccc ggccccctcc
     2161 cccgaggacc actgacggtc tccgttcgcc tcgccgcctg agccgccggc cgccccggcg
     2221 cccaggtcgg tgccggccac ttgacaccga agccgttgcc gccgccggcg gccaccagga
     2281 atcgcaccac acgcatccga ggaggggagg tcagacatga gcagctggcc atcacgcgag
     2341 ggcatgcccg acctgagcga gcagttcgag ccgctgcgac gcgccgtcca gcgcttcggc
     2401 cggcagcggc tggtcacggt ggtcgtggcc ggcgccgtac tgctgtggct cgcttctggc
     2461 ttctacgtcg tcggacccgg cgagcggggg gtcgtgctcc tgttcggtcg agctgtcgac
     2521 cagaccgagt ccggcctgcg ctaccgcctg ccgtcgccga tccagtcaca ccagatcgtg
     2581 gacgtcgcga cggtacgccg ggcggaggtc ggcttccgct cggaccgcgg cggggcccgg
     2641 gccctgccga gcgagtcgct catgctcacg ggcgacgaga acatcgtcga cgtgcagctc
     2701 ttcgtccagt acctcgtccg ggaccccgcg aagttcctgt tccgggcgcg ccatccggag
     2761 atggcgctgc actcctcggc cgaggtggcg ctccgggcgg cggtgggcga gaccaacatc
     2821 gactacacaa tgaccgaggg gcgggtcgag gtgcaggacc gcgtcaagaa gcaactccag
     2881 gagctgctgg atctctacgg caccggtctg ctcgtcaccg aggcgcgcct gctcgtggtc
     2941 gatccgccgc gggaggtcaa ggaggccttc cacgacgtcg tccgggcctg ggaagatcgc
     3001 gagcgcctca tcaaggaggc cgagggctac gccgagggcg tcgtgcccaa ggcccgcggg
     3061 gaggcggcgc agatgctgcg gactgccgag gcgtatcgcg agcagcgcct gatccgcgcc
     3121 cgaggcgacg ccgaccggtt cgtgcgcgtt cacaacgagt accggaaggc cgaggccgtg
     3181 acccgcgacc ggctctacct cgagatggtc gaccgtgtgc tgccgcgcac gaagaccgtc
     3241 gtggtggact cgagcggcga ccgcagcagc gtggtgccgc tgctgcccct ggggacgttc
     3301 ctgccggggc cgccgacggc gaagaccaac agccgcgagc cggcatccgc gccggccgcg
     3361 acgacgggag ggcgatgacc atggcctggc ggatcgcagt catcgtgctg atcaccgccg
     3421 tcctcggcgg cgcgacctac atcgtcccgg agtggcagca ggcgatcgtg acccagttcg
     3481 gccagcccat ccgcaccgtg cgggagccgg ggctgtactg gaaggtgccc ttcctgcaca
     3541 cggtgacata cttcgacaag cgagtgctgg ccgccgacac ggggggcgcg gagtacatca
     3601 cactcgacaa gaagcgcctg ctgatcgacc acatctcccg gtgggagatc gtcgacccgc
     3661 tgcagttcta tcgcaccgtc cgcgacgagc gtggcgccat ggcgcgtctg gacgacgtga
     3721 tcacggcccg gctgcgccag gaggtggcca aggagacttt caaggagatc atccgggaga
     3781 agcgcgaggg catcatggag acggtgaccg cggaggcgcg ggcgctggcg acgccgttcg
     3841 gcatccgggt gctcgacgtg cgcgtgaagc gggccgacct gccggccgag gtccaggcga
     3901 gcgtcttcgc ccgcatgcag gcggaacggc agcggatcgc gctgcgctac cgggcggagg
     3961 gggaggagaa gggccgggag atccgcgcca acgcggacaa ggagcgggag atcatcctcg
     4021 cgaaggccta ccaggagagc cagggcctgc ggggcgccgg cgacgcggaa gccaccgccg
     4081 tgaccggcca ggccttcggg cgcgatccgg ccttctacgg cttcatgcgg cgcctggaga
     4141 cgtacgagcg cgtcttcacg ccggagacga cgatcgtgct ccggcc
//
LOCUS       DATIAZ010000264         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1255674, whole genome shotgun sequence.
ACCESSION   DATIAZ010000264 DATIAZ010000000
VERSION     DATIAZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255674"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VLF19_09440"
     CDS             complement(<1..558)
                     /locus_tag="VLF19_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSE93516.1"
                     /translation="MITHDEACRLIETPRAGLDDLLARVAAVRDGDRGRRVTFSKKVF
                     VPLTTLCRDYCGYCTFRRDPGEPGAHTMTPDEVVALVEAGGRIGAKEALFSLGDKPEA
                     RFAEQRAFLRRHGHRTTLSYLRAMCALTLAESPLLPHANPGVMGERDLAALREVTVSM
                     GIMLETVSERLLGAGMAHEVAPDKVP"
     gene            complement(555..1613)
                     /locus_tag="VLF19_09445"
     CDS             complement(555..1613)
                     /locus_tag="VLF19_09445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSE93517.1"
                     /translation="MGPAMEFGLALPGRGPLATPDVVLRVAAKAEALRFSSLFVTDHV
                     VLPVSTARSVYPYSPSGQFPGGGRQDYLEPLTMLGYLAHATRRVRLGTSVLVVPYRHP
                     LTTAKMLATIDQLAGGRVILGAGAGWLREEFEALGAPPFEERGRVTDEYLTLMRLAWT
                     TDPLSFEGRYCRVRDVHVLPKPAQRGGIPVWIGGHTDAALRRAGTVGDGWHPIGLRPP
                     ALLPPDEYAAKVARLREHAKAAGRDPEAITLSLRVPMEVRGPRDKAPGGDRPLFQGTA
                     AEVAADVRRYHAAGVTHFVFDPARPDLAALLASLERFAADVRPITRATTARPARAVAG
                     PDRREARDTPERAARRRA"
     gene            1670..>2045
                     /locus_tag="VLF19_09450"
     CDS             1670..>2045
                     /locus_tag="VLF19_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE93518.1"
                     /translation="MPGSLRAWGVWSIPTLLFLIAFFHRPAPGVIAKELMQAFDATGT
                     FVGLVSATYFYAYAGFMLPAGLLIDAYGARSVVTAGGAVMGAGALLMGAATTQAVLFA
                     GRFVVGLGATVTFVGALKIAATW"
BASE COUNT          267 a          742 c          758 g          278 t
ORIGIN      
        1 gggcaccttg tccggcgcca cctcgtgcgc catgccggcc cccaggagac gctcggagac
       61 ggtctcgagc atgatgccca tgctcactgt cacctcgcgt agcgccgcca ggtcacgctc
      121 gcccatcacg ccggggttgg cgtgcggcag caggggggat tcggcgagcg tcagcgcgca
      181 catggcccgg agataagaga gcgtggtgcg gtgaccgtgg cggcgcagga acgcacgctg
      241 ctccgcgaag cgcgcctcgg gcttgtcacc gagcgagaac agggcctcct tcgcgccgat
      301 gcggccgccg gcctcgacca gcgccacgac ctcgtcgggc gtcatcgtgt gagcgccggg
      361 ctcgcctggg tcgcgacgga aggtgcagta tccgcagtag tcgcgacaga gggtcgtcag
      421 cggcacgaag accttcttcg agaaggtcac gcggcggcca cggtccccat cgcggacggc
      481 ggccacgcgc gcgagcaggt cgtccagacc ggcgcgcggg gtctcgatga ggcggcacgc
      541 ctcgtcgtgc gtgatcatgc gcggcgccgc gcggctcgct cgggcgtgtc tcgcgcctcg
      601 cggcggtccg gcccggccac ggcccgcgcc ggccgcgcgg tcgtcgccct cgtgatcggc
      661 cggacgtccg cggcgaagcg ctcgaggctc gccagcagcg ccgcgaggtc gggccgcgcg
      721 gggtcgaaga cgaagtgcgt gacgccggcc gcgtgatagc ggcgcacgtc ggcggcgacc
      781 tccgcggccg tgccctgaaa gagcgggcgg tcgccgcccg gcgccttgtc gcgcggcccc
      841 cgcacttcca tcgggacccg caagctcagc gtgatcgctt cgggatcccg gccggcagcc
      901 ttcgcgtgct cgcgcaggcg cgcgaccttc gccgcgtact cgtcgggcgg aagcagagcc
      961 ggcgggcgca gaccgatcgg atgccagccg tcgccgacgg tgccggcgcg ccggagcgcc
     1021 gcgtcggtgt ggccgccgat ccagacgggg atgccgccgc gctgggcagg cttcggcagc
     1081 acgtgcacgt cgcggacgcg acagtagcgg ccctcgaagc tcaggggatc cgtcgtccac
     1141 gccaggcgca tcagtgtcag gtactcgtcg gtcacgcgcc cgcgctcctc gaacggcggc
     1201 gcgccgagcg cctcgaactc ctcgcggagc cagcccgcgc ccgcgccgag gatcacccgg
     1261 cctccggcga gctggtcgat cgtcgccagc atcttcgccg tggtgagcgg gtggcggtag
     1321 ggcacgacga gcacgctggt gccgaggcgc acgcggcgcg tggcatgcgc caggtagccg
     1381 agcatcgtga gcggttcgag gtagtcctgc cggcccccgc ccgggaactg accgctcggc
     1441 gagtacgggt acaccgaacg agccgtcgag accggcagca cgacgtggtc cgtgacgaag
     1501 agcgacgaga agcgcagcgc ctcggccttg gcggcgaccc tgaggacgac gtcgggggtg
     1561 gcgagcggac cgcgaccggg aagcgcgaga ccgaactcca tggcggggcc catggtaggc
     1621 cacgctccag gcctttgcaa tcgcgcgcgc ccgctgctat gagtgagcga tgcccggctc
     1681 gctccgcgcc tggggcgtgt ggtcgatccc cacgctcctc ttcctcatcg ccttcttcca
     1741 tcggccggcg cccggcgtca tcgccaagga gctcatgcag gcgttcgatg cgacgggcac
     1801 attcgtcggc ctcgtctcgg ccacctactt ctacgcgtac gccggcttca tgctgccggc
     1861 cggcctgctc atcgacgcgt atggcgcccg cagcgtcgtc accgccggcg gcgcggtgat
     1921 gggcgccggc gcgctcctga tgggcgccgc caccacccag gccgtgctct tcgccggacg
     1981 cttcgtggtc gggctcggcg cgacggtgac gttcgtcggc gcgctgaaga tcgccgcgac
     2041 gtggt
//
LOCUS       DATIAZ010000265        12944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_202709, whole genome shotgun sequence.
ACCESSION   DATIAZ010000265 DATIAZ010000000
VERSION     DATIAZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202709"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VLF19_09455"
     CDS             <1..905
                     /locus_tag="VLF19_09455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSE93519.1"
                     /translation="PERRALVLGTALGGVEELERALAGDGGARRARAALYDGPAHALA
                     RRLDVRGPVLTVSTACASGATAIGLGAELLRDGRADLVVAGGYDVLCRFVMRGFDALR
                     SLTRERVRPFDRRRSGLLLGEAAALVLLAREADAPRAGLGRVLGHASMADGSHIAAPD
                     PDGRGLEASARAAMRQAGVGAGAIDFVSAHGTGTPLNDRIETDVIKRVLGPRASAIPV
                     NSIKGGLGHTMGAAATLEAIMCLLASRQGLVPPTLGFEERDPQCDLDYVPLAARPARP
                     RISLSTSAGFGGCNAALVLEGRLG"
     gene            902..1756
                     /locus_tag="VLF19_09460"
     CDS             902..1756
                     /locus_tag="VLF19_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HSE93520.1"
                     /translation="MKVAAVGVLTGWGEGISALPDDARRAAAGRTIVTLPPPAAASER
                     FRRATRECRLGVAAVDALLRAAALDRETLHGAGTALVYVTAGAYGASNRAFIDGEAAR
                     GGALHFPYTAPSAVPAEVTIEFGITGPYVTFIGGAAAAIDALWHADRLLAGGACQRAL
                     VLAVETVEECASLWARGRWLVGRPLVEAAACALLLPGAAGARAEAAGRASALETTVRR
                     RSGETLACAPLIGLALALESGEDAWNVTEHWRGHRLGFDRSAGPNPRAAAGGRTGRPW
                     SPRKCSTS"
     gene            1726..2001
                     /locus_tag="VLF19_09465"
     CDS             1726..2001
                     /locus_tag="VLF19_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HSE93521.1"
                     /translation="MEPKEVLDELKTLFVTRLRFEPRRVADVDPETPLPKGLEGSIGL
                     DSLDFIELSLAIEERFGVVVDETQDLTEHFASLGSLARFIAERAQPA"
     gene            1998..3173
                     /locus_tag="VLF19_09470"
     CDS             1998..3173
                     /locus_tag="VLF19_09470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSE93522.1"
                     /translation="MRRVVVTGLGALSPFGVGVKAYWDGIRSGSCAIRPVTLIETDGF
                     RCRLAAEVPGAVPGSARRSRADRLALAAAREAVEDAGLDARDRSETALVVGAVGGGML
                     EAEAWFWAFRRENRARPTAVLRSTLPYSHAAVVGSRLGLGGPRQTVVVACSSGAAALA
                     AGAAMIEDGAATIALAGGADALTRICFMGFNALKLLDPAPCRPFDRDRRGMSVGEGAA
                     FVVLEEADRARARGARVYAELAGAGMTSDAFHVTSPQPAGEGMARAMCAALAAAGVAP
                     AEVGYANAHGTATPQNDRIEARALRDVFGEGRLLVSSTKSMIGHTMAAAGSLEAIATV
                     LALAHETVPPTANLTTPDPAVAFDCVPGIAREAALDHAISNSFGFGGQNVTLLFRRV"
     gene            3170..4285
                     /locus_tag="VLF19_09475"
     CDS             3170..4285
                     /locus_tag="VLF19_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HSE93523.1"
                     /translation="MSTGGRGPTGAPVVITGVGTVNAAFAGGATALAAWLARPRPAVR
                     PADGFTVPVGLLPADALGGLVDETEARRLSRVSQLAVVAARLALEDADLAPCPELGLV
                     VGTEFGDLRSTMEFADGYLTVGPTGLSALLFPNTVMNTMAAATSIAVAARGLSLTLNE
                     PTVSGELAVAQAAAAVAAGRVPAALAGGVDQVDPFLSGALDDLGVPPIMRGEGATFVT
                     LETLPAARARGARVLGRVVGAAWRALPARPHGVGRRAAPRAVETALAAAGLAPDAVRG
                     VYAATNGDAQRDAWEDRLLDAALAHRPPRAALRPLLGDHAGVGPFGVAAAAWTARTGR
                     VPAPDARPAATPGPALVHGVARGGAHVALVVAGTEDA"
     gene            4282..5388
                     /locus_tag="VLF19_09480"
     CDS             4282..5388
                     /locus_tag="VLF19_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSE93524.1"
                     /translation="MRPWIVVPVFNEAATIGSVVAEAACHAPVIVVDDGSTDAGAAVA
                     RAAGADVLRHPRRLGKGQALRTGFAAARRRGASHVVTLDGDGQHAPADVPRLLAAARE
                     TPASIVVGGRITGHPGGTDVTLPGTHLNAIRVAGFFVNWASGVRVNDTQSGFRVYPVA
                     VLDELELRRGGFVFETEVLVVAARCGVALQEVPITVVPRAARRSRFRPVADGAAITAY
                     LSGPVLGRWLTEAGAGVAEIATVFDGTRRRERHGMLQVAAAFTDSPLAMGAALGAGVF
                     GRAWARVRGWWRHERRRRAGTAAVATLAAPVLLVLAAARALAGRVVPDVVTPLVRRLY
                     DQARLEPRAAAANSAGPVAGGADQVSAVAAGEAR"
     gene            5385..6653
                     /locus_tag="VLF19_09485"
     CDS             5385..6653
                     /locus_tag="VLF19_09485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSE93525.1"
                     /translation="MTHTFDVVVVGGGPGGSSVATFLARGGLRVGLFERERFPRFHVG
                     ESLMPAAMLMLERLGARAAVEARGFQVKYGASFFDQESSQAATFYFLTGQPWPNYTYQ
                     VPRADFDMVLLDNARRHGVTVFQPATVESAAFDADGVTVTVSGEDGPLGARAAVMVDA
                     SGRASFLATRLGRRRRIPNLGKVALFAHFRGAERFAGKEEGNIRIYVFDEGWCWWIPL
                     AGDLTSVGAVMHKRTVGAWDGPLEELYAEMIRRCRPVAAQLAGAERVTPVHREANFAY
                     DNAPAVGDRFVAVGDAITFVDPIFSGGVYIAMRTGQLAAEAILEAFRAGRFEARRFAA
                     YERRVRRGVAPLFRFIHNYYEPAFLDLFMRPRDYFGMYRAVLAVLSGGSFIRMTWRTR
                     ASLALLFAITRVNVWIRRRAGLPVESRLEW"
     gene            6647..7843
                     /locus_tag="VLF19_09490"
     CDS             6647..7843
                     /locus_tag="VLF19_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93526.1"
                     /translation="MVGRRGVVRALRGYAFTVAGIAIGVAGLVSLGAMSERIVRFIEG
                     GDRFVLGQISVAGGGLGPGAGFTGGGLLRATTVSAIGAVPGVQGVQPQVMLPLHAGES
                     QFMVTQDMLLGLDLAVPMPNRHYRTLPVQAGRFLREGDRGVAVVGAALAASRGLAVGG
                     RLELGGRAFEVVGVLERMLTAPDRFVVLPIADAREQWIAKDSLLRTVLASGAVALTAG
                     DLNTGVAVGWRDGEDPDAVALRIRRQVPGVNVQIPSELSRTLKTSTAFFSALLAGIAV
                     LALVIGGLSVANTIAASVFERVRDFGVKRALGATDAQLGREVLGEALAVTLSGGVCGV
                     AVAVALGLGIDRWVGQGTQQLFLFSPRLMVGAAVFAMLLGAGAAAYATLRVVRLSPAE
                     AIRRGA"
     gene            7840..8508
                     /locus_tag="VLF19_09495"
     CDS             7840..8508
                     /locus_tag="VLF19_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93527.1"
                     /translation="MILRAEGLVKAYAAPDGTAIRVLDGVDLKVGAGELVVVAGASGS
                     GKSTLLNVLGLLEDADGGQVWYGDEHVSALGRSAKSRARGRFVGFVFQSFLLLPSLTA
                     LDNVLLAARYVVGADRAAARRRALALLEELGVLARRDHYPPQLSGGEQQRVAFCRAVL
                     NDPAILLADEPTGNLDDANSRVILDSLHARASGGSAVVVVSHRSDAIEGAAAVHRLRD
                     GRLA"
     gene            8542..8970
                     /locus_tag="VLF19_09500"
     CDS             8542..8970
                     /locus_tag="VLF19_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93528.1"
                     /translation="MKAVAILVSLTLVSLTLVAAAPAAAAEPLSRTFGAPVDRVWAVA
                     EAVLKHLGWDIDKADPTVGLISTDSRRVEGEDYGVYAKGTRHRLRLQIKAVGDKRASV
                     TVERDLFKRERILFVDKDEPLKATDQTVERGLLDAIGRAL"
     gene            9023..9394
                     /locus_tag="VLF19_09505"
     CDS             9023..9394
                     /locus_tag="VLF19_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSE93529.1"
                     /translation="MAKTVVLVLIAAMIGGTGHVLLAKGMKTVGDLTEAPSHRLGAMV
                     WRAVSNPWLLLGVALQATFFFTYLTVLSRANVSLVLPMTAVDYIVVALLAQYLLGEAV
                     TPARWAGIGLIVVGVFLVSRS"
     gene            9396..11093
                     /locus_tag="VLF19_09510"
     CDS             9396..11093
                     /locus_tag="VLF19_09510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSE93530.1"
                     /translation="MSGAARIAIIAGLAAAPFLFLGLGSAPFDDPGEGMHAQIARELR
                     AGGDPLALSLAGVRYVDKPPLLYALVAGAFVIGGEREAAARAVSALAAVSAVAATAWL
                     GARLLGGPWGLLAGGALLTSSGFFAYGRYVRPDALFVAALAAGFALALAGVMDDRRAL
                     VVAGFGAFGLAGLAKDPLGAIAPPLAIGAALTLGGRARPVARWLPPAGVALATALAFG
                     WWALAEARTPGYAWYTVVDNHVLNVARARQFPDEDVPLGALEFLLIAALGAAPWALAA
                     AAAVTRLVRRRAWRDPREAPWVVLALWAVGVVGLTALSSFRLPHYGLPAYPAIALLAA
                     RGWRDASHRALAVGHALAFAVLAAACGLAASGGGAAFTRHVMSATDVATRKAAEVGQV
                     SALPWDTLWILLVATAVITGAGALALAAAPRAPVAPAAVLAAMLGLLPCVGIGLTVVS
                     SHRAAQGIAREVGRRAVADDIVLHEGPLENSGALEWYSGRRPEIVDGRRSVLAFGATL
                     PGAGERFWEPSRLRAAWAGERRVWVVTTRSPERSVAAGLPGARLAAAEGGRWLWVNH"
     gene            11093..12511
                     /gene="hpnJ"
                     /locus_tag="VLF19_09515"
     CDS             11093..12511
                     /gene="hpnJ"
                     /locus_tag="VLF19_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HSE93531.1"
                     /translation="MGDYLKTLFLHPPSFQGFDGGAGARYQARREVRSFWYPTWLAQP
                     AALVPGCRLVDAPPARLTREDVVPLARDYELAVLHTSTPSFAHDVQTAEAMKAANPAL
                     RIGFVGAHVAVQAEQALGASRAIDFVAGSEFDFTIEEVARGRPLAAVRGLAYRVNGHL
                     ARTPARAPLENMDALPFVVDVYHRDLVVEDYFIGYLLHPYVSLYTGRGCRSKCTFCLW
                     PQTVGGQRYRTRSVDHVVAEIARATRLFPGVREYFFDDDTFTDDLPRAEAIARRLGRL
                     GVTWSCNAKANVPYATLKILRDNGLRLLLVGYESGSQVVLNNVKKGVRVDGARRFTRD
                     AKTLGIKIHGTFIMGLPGETRETIEDTIRFAQAIDPDTLQVSLAAPYPGTALYREARE
                     RGWLGEDALVDGGGVQAAVIGYPHLGRTEIFASVETFYRRFYFRPRKIFAIAGEMVRD
                     PAVGLRRLKEGAEFLRFLAARR"
     gene            complement(12486..>12944)
                     /locus_tag="VLF19_09520"
     CDS             complement(12486..>12944)
                     /locus_tag="VLF19_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSE93532.1"
                     /translation="VVRAIADAGHEVGNHGWSHTSLWRCGPRRTAEEIGRAHDTLGEL
                     AGRAPRHFRPPWGMVNAAMFPLLRRFGERCVFWTVQSEGLVPRPGPAQAGYVLHRARP
                     GVIVDLHDADGVAGAGARLADALPPMIDGLRAAGYDLVTVAELIAEPREI"
BASE COUNT         1434 a         4692 c         4936 g         1882 t
ORIGIN      
        1 ctccggagcg ccgggcgctg gtgctcggca ccgcgctggg gggcgtggag gaactggagc
       61 gtgcgctcgc cggcgacggc ggtgcgcgcc gggcgcgggc ggcgctctac gacggacccg
      121 ctcacgcgct ggcccgccgc ctcgacgtcc gcggtccggt gctcaccgtc tcgacggcgt
      181 gcgcgtccgg cgccacggcg atcgggctcg gcgccgagct cctccgcgac gggcgcgccg
      241 atctcgtcgt cgccggaggt tacgacgtcc tctgccggtt cgtcatgcgg gggttcgacg
      301 ccctgcgatc cctcacgcgc gagcgcgtgc ggccgttcga ccggcggcgg agcggactgc
      361 tcctgggaga agccgcggcg ctcgtgctgc tcgcgcgcga ggcggacgcg ccgcgcgccg
      421 gcctcggccg ggtgctcggg cacgccagca tggccgacgg ctcgcacatc gccgcgccgg
      481 atcccgacgg gcgcggcctg gaggcgtcgg cccgggcggc gatgcgccag gccggcgtgg
      541 gcgccggcgc gatcgacttc gtcagtgccc acggcacggg cacgccgctg aacgatcgca
      601 tcgagaccga cgtgatcaag cgcgtgctcg ggccgcgcgc ctcggcgatc ccggtgaact
      661 ccatcaaggg cggcctcggg cacacgatgg gggcggcggc gacgctcgag gccatcatgt
      721 gcctgctggc ctcgcgtcag ggcctggtgc ccccgacgct ggggttcgaa gagcgtgatc
      781 cccagtgcga cctcgactac gtgccgctgg ccgcgcgtcc ggcgcgaccg cggatcagcc
      841 tgagcacctc ggcgggcttc ggcggctgca acgccgcgct ggtcctcgag ggccggctcg
      901 gatgaaggtc gccgcggtcg gcgtcctcac gggctggggc gaggggatct ccgccctgcc
      961 tgacgacgca cgccgggcgg cggccggccg cacgatcgtc acgcttcccc caccggccgc
     1021 cgcgagcgag cgcttccggc gcgccacgcg cgagtgccgg ctcggcgtgg ccgcggtgga
     1081 cgcgctgctg cgggcggccg cgctcgatcg cgagacgctg cacggcgccg gcaccgcgct
     1141 cgtctacgta accgcgggcg cctacggcgc ctcgaaccgc gcgttcatcg acggcgaggc
     1201 agcgcgaggc ggggcgctgc acttccccta cacggcgccg agcgccgtgc ccgcagaggt
     1261 cacgatcgag ttcgggatca ccggcccgta cgtgacgttc atcggcggcg ccgcggccgc
     1321 gatcgacgcg ctctggcacg cggatcgatt gctcgccggc ggcgcatgcc aacgggcgct
     1381 cgtgctggcg gtggagacgg tcgaggagtg cgcgagcctg tgggcccggg gccgctggct
     1441 cgtcggtcgg ccgctggtgg aggccgccgc gtgcgcgctg ctgctgccgg gggctgcggg
     1501 cgcccgcgcg gaggccgctg gccgcgcgtc ggcgctcgag acgaccgttc ggcggcggtc
     1561 cggtgagact ctcgcctgtg ccccgctgat cgggctggcg ctggcgctcg agtcaggcga
     1621 ggatgcctgg aacgtgacgg aacactggcg gggccatcgc ctcgggttcg accggagcgc
     1681 ggggcccaac cccagagccg ccgcgggcgg cagaacaggg aggccatgga gcccaaggaa
     1741 gtgctcgacg agctgaagac gctgttcgtg acccgcctca ggttcgaacc tcggcgggtg
     1801 gccgacgtgg acccggagac gccactgccc aagggccttg agggctcgat cgggctcgac
     1861 tcgctcgatt tcatcgagct gagtctcgcg atcgaggagc gcttcggcgt ggtggtggac
     1921 gagacgcagg acctgacgga gcacttcgcc tcgctcggct cgcttgcccg cttcatcgcg
     1981 gagcgcgccc agccggcatg agacgggtcg tcgtcaccgg cttgggggca ctgagcccct
     2041 tcggcgtcgg cgtgaaggcc tactgggacg gtatccgctc gggcagctgt gcgattcgtc
     2101 ccgtcacgct gatcgagacc gacggctttc gctgtcgcct cgcggccgag gtgccggggg
     2161 cggtgccagg ctcggcccgc cgatcgcgcg ccgaccggct ggcccttgcg gccgcgcgcg
     2221 aggcggtgga ggacgcgggg ctggacgcgc gtgatcgctc ggagaccgcg ctcgtggtgg
     2281 gcgcggtggg cggcgggatg ctggaggccg aggcgtggtt ctgggccttc cggcgcgaga
     2341 accgcgcacg tccgacggcc gtgctccgat cgacattgcc gtactcccat gcggcggtgg
     2401 tgggcagccg gctgggcctc ggcggcccac ggcagaccgt cgtcgtcgcc tgcagctcgg
     2461 gcgcggcggc gctggccgcg ggtgccgcga tgatcgagga cggcgccgcc acgatcgccc
     2521 tggcgggtgg cgcggacgcg ctcacgcgca tctgtttcat ggggttcaac gcgttgaagc
     2581 tcctcgaccc ggcgccctgc cgcccgttcg accgcgatcg ccggggcatg tccgtcggcg
     2641 agggcgcggc gttcgtcgtg ctcgaggagg cggaccgcgc ccgcgcgcgt ggcgcccgtg
     2701 tctacgccga gctggccggc gcggggatga cgagcgacgc ttttcacgtg acctcgcccc
     2761 agcccgcggg cgagggcatg gcgcgagcga tgtgcgctgc gctggcggct gcgggcgtgg
     2821 cgcccgcgga ggtcggctac gccaacgcgc acggcaccgc gacgcctcag aacgaccgca
     2881 tcgaggcgcg tgcgctgcgc gacgtgttcg gggagggccg gctgctcgtc agctccacca
     2941 agtcgatgat cggccacacc atggccgccg ccggcagcct cgaggcgatc gcgacggtgc
     3001 tcgccctggc ccacgagacg gtgccaccga cggcgaacct gacgacgccg gaccccgcgg
     3061 tggcgttcga ctgcgtgccg ggcatcgcgc gcgaggcggc gctcgatcac gcgatctcca
     3121 actcgttcgg cttcggcggc cagaacgtga cgctgctctt ccgccgcgta tgagcacggg
     3181 cgggcggggc cccacgggcg cgcccgtcgt gatcaccggc gtcggcacgg tgaacgcggc
     3241 gttcgcgggc ggcgcgaccg cgctcgccgc ctggctggcg cggcctcgtc cggcggtacg
     3301 gccggccgat gggttcaccg tgccagtggg tctgctgccc gcggacgcgc tcggcggcct
     3361 cgtcgacgag acggaggcgc ggcgactctc gcgggtctcc cagctcgccg tcgtggcggc
     3421 gcgcctggcc ctggaggatg ccgacctcgc gccgtgcccc gagctcggac tggtcgtcgg
     3481 caccgagttc ggtgatctcc ggtccacgat ggagttcgcg gacggctatc tcacggtcgg
     3541 gccgaccgga ctgtcggcgc tgctgttccc gaataccgtg atgaacacga tggccgccgc
     3601 gacgtcgatt gccgtcgccg cccgcggcct ctcgctgacg ctcaacgagc ccacggtctc
     3661 gggcgagctg gcggtggccc aggccgcggc cgccgtcgcc gcggggcgcg tgcccgcggc
     3721 gctggccggg ggtgtcgacc aggtggaccc cttcctctcc ggcgcgctcg acgacctcgg
     3781 cgtgccgccc atcatgcggg gcgagggcgc gacgttcgtc acgctcgaga cgctcccggc
     3841 agcccgcgcc cggggcgcgc gcgttctcgg acgcgtcgtg ggcgccgcgt ggcgcgcgct
     3901 gcccgcgcgt ccccacggtg tcgggcggcg ggctgcaccg cgtgcggtgg agacggcgct
     3961 cgccgccgcc ggcctggcgc cggatgccgt gcgcggcgtg tacgcggcga cgaacggcga
     4021 cgcccagcgc gacgcgtggg aagatcgcct gctcgacgcc gcgctcgctc accgcccgcc
     4081 gcgggcggcg ctgcgcccgc tgctcgggga tcacgcgggt gtggggccgt tcggcgtggc
     4141 cgccgcggcg tggacggcgc gcaccggacg ggtgcccgcc cccgacgcgc gccccgcggc
     4201 caccccgggg ccggcgctcg tccacggcgt ggcgcggggg ggcgcgcacg tggccctcgt
     4261 cgtcgccggc acggaggacg cgtgagaccg tggatcgtcg tgccagtgtt caacgaggcg
     4321 gcgacgatcg gcagtgtcgt cgcggaggcc gcctgccacg cgccggtgat cgtcgtggac
     4381 gacggatcga cggatgccgg agcggcggtc gcgcgcgcgg ccggggccga cgtgctccgg
     4441 cacccgcgcc gtctgggcaa gggccaggcg ctcaggacgg gattcgccgc ggcgcggcgg
     4501 cgcggagcct cccacgtcgt gacgctcgac ggtgacggtc agcacgcgcc cgcggacgtc
     4561 ccgcggcttc tcgcggccgc gcgcgagacc ccggcgagca tcgtcgtcgg cggccggatc
     4621 accggccacc ccggcggcac cgacgtgacg ctgccgggga cgcacctcaa cgccatccgt
     4681 gtcgccgggt tcttcgtgaa ctgggcgagc ggtgtccgcg tgaacgacac gcagtcgggg
     4741 ttccgcgtgt atcccgtggc cgtgctcgac gagctcgagc tgcggcgtgg cggcttcgtc
     4801 ttcgagaccg aggtgctcgt cgtcgctgcc cgatgcgggg tggccctgca agaggtcccg
     4861 atcacggtgg tgccgcgcgc ggcgcgccgg agtcgcttcc gtccggtggc cgacggcgcg
     4921 gcgatcaccg cctacctgag cggtccggtg ctggggcgat ggctgacgga ggcgggcgcg
     4981 ggcgtggccg agatcgccac ggtcttcgat gggacgcggc gtcgcgagcg gcacgggatg
     5041 ctgcaggtcg cagctgcgtt caccgactcg ccgctcgcca tgggcgcggc gctcggcgcc
     5101 ggcgtcttcg gccgcgcgtg ggctcgcgtg cgcggctggt ggcgccacga gcgccggcgc
     5161 cgggccggca cggcggccgt cgcgacgctg gcggccccgg tgctgctggt cctggcggcg
     5221 gctcgggcgc tcgcgggccg cgtggtcccg gacgtggtga cgccgctcgt gagacggctc
     5281 tacgaccagg cgcgactcga gccgcgagcg gccgcggcga actccgccgg gcctgtggcc
     5341 ggcggcgcgg accaggtgtc ggccgtggcc gccggggagg cccgatgacg cacaccttcg
     5401 acgtcgtggt ggtgggtggc gggcccggcg gctcgagcgt cgccaccttc ctcgcccggg
     5461 gcggtctccg cgtgggtctc ttcgagcgcg agcgttttcc tcgcttccat gtcggtgagt
     5521 cgctgatgcc ggcggcgatg ctgatgctgg agcggctcgg ggcccgcgcc gccgtcgagg
     5581 ctcgaggctt tcaggtcaag tacggggcca gcttcttcga ccaggagtcc tcgcaggcgg
     5641 cgacgttcta tttcctcacc ggccagccgt ggccgaacta cacctaccag gttccgcgcg
     5701 ccgacttcga catggtcctg ctcgacaacg cgcggcgcca cggcgtgacg gtcttccagc
     5761 cggccacggt cgagtcggcg gccttcgacg ccgacggcgt gacggtgacg gtctccggcg
     5821 aggacggccc gctcggcgcg cgggcggcgg tcatggtcga cgccagcggg cgcgcgagct
     5881 tcctcgccac gcggctcgga cgacgccggc gcatccccaa cctcggcaag gtggctctct
     5941 tcgcccactt ccgcggggcc gagcgattcg ccggcaagga agagggcaac atccggatct
     6001 acgtcttcga cgagggctgg tgctggtgga tcccgctcgc cggagacctg acgagcgtcg
     6061 gcgccgtcat gcacaagcgg acggtgggcg cctgggacgg accgctcgag gagctgtacg
     6121 ccgagatgat ccgacgctgc cggccggtcg ccgcgcagct ggccggcgcg gagcgggtca
     6181 cgcccgtgca ccgcgaggcg aacttcgcct acgacaacgc gcccgcggtg ggcgaccggt
     6241 tcgtggccgt cggcgacgcg atcacgttcg tggacccgat cttctcgggc ggtgtctaca
     6301 tcgccatgcg gaccgggcag ctcgcggcgg aggcgatcct cgaggcgttc cgcgccggac
     6361 gcttcgaggc gcggcggttc gcggcctacg agcgccgcgt gcgtcgcggc gtcgcaccgc
     6421 tcttccgctt catccacaac tactacgagc cggcgttcct cgacctcttc atgcggcccc
     6481 gcgactactt cggcatgtac cgcgcggtgc tcgccgtgct ctcgggcggc tccttcatcc
     6541 ggatgacgtg gcgcacgcgg gcgtcgctgg cgctgttgtt cgcgatcacg cgcgtgaacg
     6601 tctggattcg ccggcgcgcc ggcctccccg tcgagtcaag gctggagtgg tagggcggcg
     6661 cggcgtcgtc cgggcgctgc gcggctacgc cttcaccgtc gccggcatcg ccatcggcgt
     6721 ggccgggctc gtgtccctcg gcgccatgag cgagcgcatc gtgcgcttca tcgagggcgg
     6781 cgaccgcttc gtcctcggcc agatctccgt cgccggcggg gggctcggcc ccggcgccgg
     6841 cttcaccgga ggcggcctgc tccgcgccac caccgtcagc gcgatcggcg cagtccccgg
     6901 tgtccagggc gtgcagcccc aggtcatgct gccgctccat gccggggagt cgcagttcat
     6961 ggtcacgcag gacatgctcc tgggcctcga tctcgccgtg ccgatgccga accgtcacta
     7021 ccggacgctt cccgtgcagg ccggccggtt cctccgcgag ggcgaccggg gcgttgcggt
     7081 ggtcggggcc gcgctcgcgg cgtcccgggg actcgccgtc ggcgggcggc tcgagctcgg
     7141 cggccgcgcc ttcgaggtcg tcggcgtgct cgagcggatg ctcacggcgc ccgaccgatt
     7201 cgtcgtcctg ccgatcgccg acgcgcgcga gcagtggatc gccaaggaca gcctcctccg
     7261 gacggtcctc gcctccggcg ccgtggcgct gaccgccggc gatctcaaca ccggcgtggc
     7321 cgtgggctgg cgcgacggtg aggatccgga cgccgtggcc ctgaggatcc gacggcaggt
     7381 gcccggcgtg aacgtgcaga tccccagcga gctcagccgc acgctgaaga cctccacggc
     7441 attcttttcc gcgctgctcg cgggtatcgc cgtcctcgcc ctcgtcatcg gcgggctcag
     7501 tgtcgccaac accatcgccg cgtcggtgtt cgagcgggtc cgtgacttcg gcgtcaagcg
     7561 tgcgctggga gcgaccgacg cacagctcgg ccgcgaggtg ctcggcgagg ccctggcggt
     7621 gacactctcg ggcggcgtct gcggcgtcgc cgtcgccgtc gcgctgggcc tcgggatcga
     7681 ccgctgggtg ggacagggca cgcagcagct cttcctcttc tccccccggc tgatggtcgg
     7741 cgcggccgtc ttcgccatgc tcctcggggc cggcgccgcc gcgtacgcca ccctccgcgt
     7801 cgtgcggctg tcgccggccg aggcgatccg gcgcggcgcg tgatactccg ggccgaggga
     7861 ctggtcaagg cgtacgcggc gccggacggg acggcgatcc gggtgctcga cggcgtggac
     7921 ctgaaggtcg gcgccggaga gctggtggtc gttgccggcg cgtcgggctc cggcaagagc
     7981 acgctcctca acgtcctggg cctgctggag gacgccgatg gcgggcaggt gtggtacggc
     8041 gacgagcacg tgagcgcgct cgggcggtcg gcgaagagcc gcgcgcgggg ccggttcgtc
     8101 ggcttcgtgt tccagtcgtt cctgctcctg ccgtcgctca ccgcgctcga caacgtgctg
     8161 ctcgccgcac gctacgtcgt cggtgccgac cgcgccgcgg cccgccgccg cgcgctggcg
     8221 ctgctggagg agctgggcgt cctcgcgcgg cgtgatcact atccgccgca gctctcgggc
     8281 ggcgagcagc agcgcgtcgc cttctgccgg gccgtcctca acgatccggc gatcctgctc
     8341 gccgatgagc cgacgggcaa tctcgacgac gccaacagcc gcgtcatcct ggactcgctc
     8401 cacgcccgcg cgagcggcgg cagcgccgtc gtcgtggtga gccaccggtc cgacgcgatc
     8461 gagggagctg ctgccgtcca ccggctgcgt gatggtaggc tcgcctaggt tgcctagggc
     8521 cggagcacgg ggagggtggg aatgaaggcc gtcgccattc tggtgtctct cacgctggtg
     8581 tcgctcacgc tggtcgccgc cgcgccagcg gccgccgccg agccgctgtc gcgcacgttc
     8641 ggcgcgccgg tggaccgcgt ctgggccgtg gccgaagcgg tgctgaagca cctcggctgg
     8701 gacatcgaca aggctgaccc cacggtcggg ctcatcagca ccgactcgcg acgcgtcgag
     8761 ggcgaggact atggcgtgta cgcgaagggg acccgccacc gactgcgtct gcagatcaag
     8821 gcggtcggcg acaagcgcgc gagcgtcacc gtggagcgcg acctcttcaa gcgcgagcgg
     8881 atcctgttcg tggacaagga cgagccgctc aaggccaccg accagaccgt cgagcgcggg
     8941 ttgctcgatg cgatcgggag ggccctgtga ggccacccgt ccctgcgcgg gacgcgccgt
     9001 ccgcgatgcg gaaggcgccc tgatggcgaa gaccgtggtc ctcgtcctga tcgcggccat
     9061 gatcggtggc acgggccacg tgctgctcgc caagggcatg aagaccgtcg gcgacctcac
     9121 cgaggccccg agccatcggc tcggggccat ggtctggcgc gcggtgtcga acccctggct
     9181 cctcctgggc gtcgcgctcc aggcgacctt cttcttcacg tacttgacgg tgctgtcgcg
     9241 cgccaacgtg agcctcgtgc tcccgatgac ggccgtcgac tacatcgtgg tcgcgctgct
     9301 ggcccagtac ctgctcggcg aggccgtcac gcctgcccgg tgggcgggca tcgggctgat
     9361 cgtggtcggt gtcttcctgg tctcgcggtc ctgaggtgag cggcgccgcg cgcatcgcga
     9421 tcatcgccgg gctggccgcg gctccgttcc tgttcctggg gctcgggtcc gctcccttcg
     9481 acgatcccgg cgagggcatg cacgcccaga tcgcccgcga gctgcgcgcg ggtggcgacc
     9541 cgctggcgct gtcgctggcc ggcgtccgat acgtggacaa gccgccgctg ctctacgcgc
     9601 tcgtcgccgg cgccttcgtg atcggtggcg agcgcgaggc ggctgcgcgc gcggtgtccg
     9661 cgctggcggc ggtctccgcg gtcgcggcga cggcgtggct gggcgcgcgc ctgctcggcg
     9721 gcccctgggg gctcctggcc ggtggtgctc tgctcacgag ctccggcttt ttcgcctacg
     9781 gccgttacgt gcgccccgac gcgctcttcg tcgccgcgct cgccgcgggc ttcgctctgg
     9841 cgctcgcggg tgtgatggac gatcgtcggg cgctcgtcgt cgcgggcttt ggcgctttcg
     9901 ggctcgccgg gctcgccaag gacccactcg gcgccatcgc gccgccgctg gcgatcgggg
     9961 cggcgctgac gctcggcggc cgcgcccgcc ccgtcgcgcg ctggctgccg ccggcgggcg
    10021 tggcactcgc caccgcgctc gccttcgggt ggtgggcgct cgccgaggcg cgcacgcctg
    10081 gttacgcctg gtacacggta gtggacaacc acgtgctcaa cgtcgcgcgc gcgcggcagt
    10141 tccctgacga ggacgtgcca ctcggcgcgc tcgagttcct cctgatcgcc gcgctcgggg
    10201 ccgcgccctg ggcgctggcg gcggcggccg cggtgacgcg cctggtccgg cgtcgcgcgt
    10261 ggcgcgaccc gcgcgaggcg ccctgggtgg tgctcgcgct gtgggcggtg ggggtcgtcg
    10321 ggctcacggc gctctcgtcg ttccgcctgc cgcactacgg gctgccggcg tatccggcca
    10381 tcgcgctgct cgccgcgcgc ggctggcggg acgcgagcca tcgcgcactg gccgtcgggc
    10441 acgcgctcgc gttcgccgtg ctcgcggcgg cgtgcgggct cgctgccagc gggggcggcg
    10501 ccgccttcac gcgtcacgtc atgtcggcga ccgacgtggc cacgcgaaag gcggcggagg
    10561 tcggccaggt ctcggccctg ccgtgggaca ctctctggat cctgctcgtg gccacggcgg
    10621 tgatcaccgg cgccggcgcg ctcgcgctcg ccgccgcgcc gcgcgcgccg gtggcgccgg
    10681 ccgcggtgct ggccgcgatg ctcggactgc tgccatgcgt gggcatcggc ctcaccgtgg
    10741 tctccagcca ccgcgccgcg caggggatcg cgcgggaggt aggccggcgc gcggtcgcgg
    10801 acgacatcgt cctgcacgag ggcccgctcg agaactccgg cgcgctcgag tggtactcgg
    10861 gccggcgtcc ggagatcgtg gacggccggc gcagcgtgct cgccttcggc gccaccctcc
    10921 cgggcgccgg cgagcggttc tgggagccgt cgcgcctgcg ggcggcctgg gcgggagagc
    10981 gccgcgtgtg ggtggtcacc acgcgctcgc ccgagcgcag cgtcgccgcg ggcctgcccg
    11041 gcgcgcggct ggccgcggcc gagggcggcc gctggctctg ggtcaaccac tgatgggcga
    11101 ttacctcaag accctgttcc tccacccgcc gtcgtttcag ggcttcgacg gcggcgccgg
    11161 cgctcgctac caggcgcgcc gcgaggttcg ctcgttctgg tatcccacgt ggctcgcgca
    11221 gccggcggcg ctggtgcccg gctgccgtct ggtggacgca ccgccggcgc gactcacccg
    11281 cgaggacgtg gtgccgctcg cccgcgacta cgaactggcg gtgctgcaca cctcgacgcc
    11341 gtcgttcgct cacgacgtgc agaccgcgga ggcgatgaag gcggcgaacc cggcgctgcg
    11401 gatcggattc gtgggcgcgc acgtcgccgt gcaagccgag caggcgctcg gagcctcgcg
    11461 ggccatcgac ttcgtcgccg gcagcgagtt cgacttcact atcgaggagg tcgcgcgcgg
    11521 ccggcccctg gcggcggtcc gcgggctcgc ctaccgggtg aacggccacc tcgcccggac
    11581 acccgctcgc gccccgctcg agaacatgga cgcgctgccc ttcgtcgtgg acgtctacca
    11641 ccgcgatctc gtggtcgaag actacttcat cggctacctg ttgcaccctt acgtctcgct
    11701 ctacaccggg cgcggctgcc gatcgaagtg caccttctgc ctctggccgc agaccgtggg
    11761 ggggcagcgc taccgcaccc ggagcgtgga ccacgtcgtc gccgagatcg cgcgggccac
    11821 gcgcctgttc cccggcgtca gggagtactt cttcgacgac gacacgttca cggacgacct
    11881 gccccgcgcg gaggcgatcg cacgcaggct cggtcgtctc ggggtgacgt ggtcgtgcaa
    11941 cgccaaggcc aacgtcccct acgcgacgct gaagatcctg cgcgacaatg gtctgcgtct
    12001 gctactggtc ggctacgagt cgggcagcca ggtcgtcctc aacaacgtga agaagggcgt
    12061 gcgcgtggac ggcgcgcgac ggttcacgcg cgacgccaag accctcggca tcaagatcca
    12121 cggcacgttc atcatggggt tgcccggcga gacgcgggag acgatcgagg acaccatccg
    12181 cttcgcgcag gcaatcgatc ccgacacgct ccaggtctcg ctcgccgccc cgtacccggg
    12241 tacggcgctg taccgcgaag cccgcgagcg gggatggctc ggggaggacg ccctcgtgga
    12301 cggcggcggc gtgcaggcgg ccgtcatcgg ctatccgcac ctgggccgca cggagatctt
    12361 cgcctccgtc gagacattct accggcgctt ctacttccgg ccgcggaaga tcttcgcgat
    12421 cgccggcgaa atggtccgcg atccggccgt ggggctgagg cggctgaagg aaggcgccga
    12481 attcctcaga tttctcgcgg ctcggcgatg agctcggcga cggtgacgag gtcgtagccg
    12541 gcggcccgca ggccgtcgat catcggcggc agggcgtcgg ccagccgggc gccggcgccc
    12601 gccacgccgt cggcgtcgtg caggtcgacg atgactcccg gccgcgcgcg gtggagcacg
    12661 tacccggcct gcgcaggacc gggtcgcggc accagcccct cggactggac ggtccagaac
    12721 acgcagcgct cgccgaaccg ccggagcagg ggaaacatcg cggcgttcac catgccccac
    12781 ggcgggcgga agtggcgtgg tgcgcggccc gcgagctctc cgagcgtgtc gtgggcgcgc
    12841 ccgatctcct ccgccgtgcg ccgcggcccg catcgccaga ggctcgtgtg ggaccagccg
    12901 tggttgccga cctcgtggcc cgcgtcggcg atggcgcgga cgac
//
LOCUS       DATIAZ010000266        12741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_731536, whole genome shotgun sequence.
ACCESSION   DATIAZ010000266 DATIAZ010000000
VERSION     DATIAZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12741
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731536"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VLF19_09525"
     CDS             <1..1382
                     /locus_tag="VLF19_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSE93533.1"
                     /translation="SAGGVALPDDARRFPIRLVESGPAAGALAAAQAARERGEAHLLS
                     FDMGGTTAKACVIDGGTPLVAREFEVARADRFKKGSGLPIRVPVIELIEIGAGGGSVA
                     RVDRMGLLKVGPDSAGADPGPACYSLGGRMPTVTDADLLLGYLDADFFLGGRMRLDRD
                     AAHRAIDEHVARPLGLDPVDAAWGIHRVVNENMAAAARIHGIERGRDLRQYPLFAFGG
                     AGPVHCWQVAQILRMPRILLPFGAGAMSAYGLLAAPLAFDFVRTRRQHLDAADWTAVN
                     ALFGEMEAEGRAVLGRAGVAEADVRVSRVAEMRYLGQGHEVEATLPRGDLGPASLPAI
                     REAFEVAYRALYHRLPQGVPIEALNWRVTVAGPRPEVVFRPSGALSAPAPAGGATWHS
                     AVKTHRRAWFAEAAGFIETPVYDRYALGAGATFEGPAIVEERESTAVIGPGGRCRVDD
                     GLVIIAELP"
     gene            1426..1674
                     /locus_tag="VLF19_09530"
     CDS             1426..1674
                     /locus_tag="VLF19_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93534.1"
                     /translation="MIGVVGDFNPANPTHGFTNAALEHLGLSFEWRATDAVGVHPEQS
                     LEPYDGLWIAPASPYRSMDGALQAIRYARERGVPLVGT"
     gene            1897..2121
                     /locus_tag="VLF19_09535"
     CDS             1897..2121
                     /locus_tag="VLF19_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93535.1"
                     /translation="MNRAYEQLLEDGGLRISGVGVEGEVRIVEIPEHPFFVATLFLPQ
                     ARSAPAAPHPLLAGFAAATASRHAERVQRP"
     gene            2132..3721
                     /locus_tag="VLF19_09540"
     CDS             2132..3721
                     /locus_tag="VLF19_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSE93536.1"
                     /translation="MDPVTLEILWSRLIGVANEQATALQRTSFTSIVREAGDLSAGVF
                     DRRGFMIAQAVTGTPGHINSMALAMKHVLAEHPADTLRPGDVVITNDPWKTSGHLNDV
                     TIASPVFRSDDCVAFFASTCHTADIGGHVFSAEAREVYEEGLFIPIMKLYEAGRANAS
                     LIALIRANVRLPEMVLGDFHAQIAGGAVGGERLLEFMDEFGLERLEPLGDEIIARTER
                     AMREAIRALRPGVYEHAITSDGFDDPITIKARCEVRGDELTIDYAGSSPASPRGINVV
                     MNYTEAYTTYGVKVIVSPDVPNNEGAFRPLRIAAPEGSILNVCHPAAVAARHVIGHFL
                     PHVVAGALGQALPDRVMAEGSANIWGVQIAGRDLEGRPFTHVFFSSGGTGARPGKDGL
                     DATAFPSGVLGTPAEVLESIAPVLVERKALRQGSGGEGRYRGGLGQTIAFRVRTREPF
                     TCSVLCDRTRHPARGFLGGGPGAVGEVLIDGVPPDNPKAEQLVTPGALVEVRLPGGGG
                     YGPPAERDPDLLERDRLEGYV"
     gene            complement(3970..5328)
                     /locus_tag="VLF19_09545"
     CDS             complement(3970..5328)
                     /locus_tag="VLF19_09545"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE93537.1"
                     /translation="MQPAQILIVDDERSILMLLQEALTQWGYHVTTAGTAQQAVEALK
                     TQVFDAALTDVRMPDMSGLDLLKEIKKRDESIEVVIMTGYPTISSAIEALKEGAYDYL
                     SKPLILDELRMLMQRLMERRFLRGEVHSLRARLGEELTVNELIGSSPVMQRTKEIIGK
                     VAVTDSPVLVEGESGTGKELVAAAIHRLSSRAKGPFIPVNCSAIPRDLLESEFFGHVR
                     GAFSGAVADALGLFRGANEGTIFLDEIAELPPELQVKLLRVLQEMQVRPVGSTKAFPV
                     DVRVIAATNRNLEQAMHAGTFRQDLFYRLNVIRIVLPPLRERRDDIPPLVNHFIRRFN
                     KRFHRDVRGITPDALSALTAYDFPGNVRELENLIERAFALGSREQLTLADLPSLAART
                     AAVAVPSALESKSVPTLAEVEKDLILRALAVHNNDKEEAARALGISRRTIYRRLKEYG
                     RL"
     gene            5452..5781
                     /gene="trxA"
                     /locus_tag="VLF19_09550"
     CDS             5452..5781
                     /gene="trxA"
                     /locus_tag="VLF19_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSE93538.1"
                     /translation="MADSATLHLTEQNFDEALVANQGMLMVDFWAEWCGPCRAIAPVL
                     DEMVRASAGRVALAKVNVDENPGLAARYGIRSIPTILFIKDGKVVDQVIGAVPKAQLQ
                     KKLDTLA"
     gene            5806..7347
                     /gene="trpE"
                     /locus_tag="VLF19_09555"
     CDS             5806..7347
                     /gene="trpE"
                     /locus_tag="VLF19_09555"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HSE93539.1"
                     /translation="MSTGGTAVPSLSPTRDEFRALAGRGNLVPVYAELPADLDTPLSA
                     YLRLRPGPYAFLLESVEGGEKWGRYSFLGTDPLMVLSAKNGRMTLRRAGGRVVERLPD
                     GNPLDALRGLLREFEPVAVPGLPRFQGGAVGYFSYDMVRHMERLPATVQDDLRLADAI
                     FMFTDTLLVFDNLRHRLMVIANARIEGRDDASLDQAYDRAALRIGMTLAKLGRPARPP
                     APLALAAPGPLVDLGEEGFTSTMDEATFVDAVRRAKEYIAAGDAYQVVLSRRLDCPLP
                     ADPFTIYRALRTINPSPYLFFLRLGRATVVGSSPEVLVRLEGDRVEERPIAGTFPRGA
                     TEAQDKAIEEALLADPKERAEHVMLVDLGRNDVGRVTTLGSVKVTEFMGVERYSHVMH
                     LVSHVVGTLRPGADAIDVLQACFPAGTLTGAPKIRAMEIIDELEPTQRGPYGGAVGYI
                     SYSGNLDSCITIRTVVCHGNRASIQVGAGIVADSDPKTEWLETSSKARGLVLALRMAA
                     ATARL"
     gene            7344..7910
                     /locus_tag="VLF19_09560"
     CDS             7344..7910
                     /locus_tag="VLF19_09560"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSE93540.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELGAELKVARNDELDVAAVAALGP
                     ERIVISPGPGTPDDAGVSIEVIRQLGGTIPILGVCLGHQAIGQAFGATVARAHRQMHG
                     KVSSIAHDDAGVFRGLDNPFVATRYHSLAIVRSTVPADLEVSAWSEDDGEIMGVRHRR
                     HPIEGVQFHPESILTLEGKKLLRNFLKR"
     gene            7907..8956
                     /gene="thiL"
                     /locus_tag="VLF19_09565"
     CDS             7907..8956
                     /gene="thiL"
                     /locus_tag="VLF19_09565"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSE93541.1"
                     /translation="MTRGEPPERLTEASLIRAVRRRVEGGSGVQVGIGDDCAVLEPTP
                     GTRLIATTDLLLEDVHFRRRYAEPADVGWKALAVNLSDVASMGARPRWALVALACPAS
                     TTLDEVEAFYAGCLALAAEHGVAVVGGDTSASPAGWLVNITVLGEATRAPLLRSGARA
                     GDIVAVTGGLGRSAAGLAVLERATAPPGVGAEALAEVTAAHLRPRPRVAEGQWLAGVG
                     GVTAMMDLSDGLATDLAHIAEESGAGARVELERVPVAPAARLVAERIGADALAWATGG
                     GEDYELLVVCDPEAFPRLHEGSPRATGTALTRVGEMTAGTGITFMDAAGRERAVAAGF
                     EHFVTGHRGARDHRR"
     gene            8953..10227
                     /locus_tag="VLF19_09570"
     CDS             8953..10227
                     /locus_tag="VLF19_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSE93542.1"
                     /translation="MNDSVVLALLILLLLVVLSALLNGAEAAYFSLGRARLRRLRGQQ
                     PPEPGRVPLIERPHDLLVTLLVGITLTNIAASALAAAVTERIVGPGYAVLIETVVMVL
                     VLTTFTEVLPMTVAVKRPELVLAVGGRLVAWLALLLAPVRVVLGALSAVTVRLIGGER
                     RPSKELSEEELRTLVDVGASEGVVEREEREMIHKVFELEDTLVRSIMVPRTDMFCLDV
                     ATPVEAILPALRENLHSRVPVYEGSIDVIVGVLYTKDLLPYVDGLPPGFDLRAHIHPP
                     YFVPESKRADVLLQELQAKHLHMAIVVDEYGGTAGLVSLEDLIEELVGEIADEYDEPE
                     RLIQRLDATTYRVAGKLPIDELNAVTGLAISNEGYDTVGGWVLDLFGRVPRKAERLEM
                     PGLAVTVEKVERTRVVEVLVSLRKPVGGEAAA"
     gene            10224..11474
                     /locus_tag="VLF19_09575"
     CDS             10224..11474
                     /locus_tag="VLF19_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSE93543.1"
                     /translation="MTLLWIAVFCVVATMFFSMAEMAFIAANRLKLRHLAAEGDRTAA
                     LYLEAFQRPERTLATAKMGVPIAHVVASSAATFALLPVLGGIAPLVVTAVLTPIMLIV
                     GEIIPKAVAREWATTLILWLYRPLTAAAVVLAPFVVFSNFAVTALLRLAGVRQAEARQ
                     FVSREELKALLALEPGEADVTTQEAELIGKIFDLGDTTVREIMVPLVDVVLLPETATP
                     RDAIALIRERGFSRIPVYRLRESNVVGVVSAMDLLRRGAGATRLDELMREPYYVPETK
                     RIDDLLREMQRTRVQLAVVVDEYGGATGLVTLEDIIEQIVGEIRDEHDREPTSVERLR
                     DGSYWVAARVNIDEINETLDWTLPKHDYETVAGLVLAQLGRIPRRGEQFQVAGYTITV
                     LEADARRVGAVKVEPPRPARVEPR"
     gene            11516..12190
                     /locus_tag="VLF19_09580"
     CDS             11516..12190
                     /locus_tag="VLF19_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSE93544.1"
                     /translation="MAGKMVDFPSNGGKTAAYLATPPSGKGPGVLVIQEWWGLVGHIK
                     QVCDRFATEGFTALAPDMYHGQTASEPDGAGKLFMALNIAQAEKDLRGAVDYLAQQAS
                     TKKVGAVGFCMGGQLALFAGTVNPKVGAVVDFYGIHPNVKPDYTKLQGPVLGLFAEKD
                     TFVTPQTARDVDAAIKKAGKQCEIHIYPNADHAFFNDERPDAYDKAAAEDAWRRTLGF
                     FRKHLA"
     gene            complement(12278..>12741)
                     /locus_tag="VLF19_09585"
     CDS             complement(12278..>12741)
                     /locus_tag="VLF19_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93545.1"
                     /translation="WFFGDAPFLAALGAEPREAYLSRRLDYYPYYRLINDGLPAGSTV
                     WLINVRRDAYHLERPFVGDYLFEDHTIRRWVLESRDAREVRARAAAAGITHVFVRHDA
                     LLDYARSSVVDDRLPEAENRARLDRLRAFLLDGTRVLRGDGKFLLVELPRG"
BASE COUNT         1833 a         4527 c         4504 g         1877 t
ORIGIN      
        1 agtccgcggg cggcgtcgcg ctacccgacg acgcgcgccg ctttcccatt cgcctggtcg
       61 agtccgggcc cgccgccggc gcgctggcgg cggcgcaggc ggcgcgcgag cggggagaag
      121 cgcacctgct gtcgttcgac atgggcggca ccaccgccaa ggcgtgcgtc atcgacggcg
      181 gcacgccgct ggtcgcgcgc gagttcgagg tggcgcgcgc ggaccgcttc aagaagggct
      241 ccgggctgcc catccgcgtg cccgtcatcg agctgatcga gatcggcgcc gggggcggct
      301 cggtcgcacg cgtggaccgg atggggctcc tcaaggtcgg gcccgacagc gccggcgccg
      361 accccgggcc cgcatgctac agcctcggcg gccggatgcc gacggtgacc gacgccgacc
      421 tcctcctcgg ctacctcgac gccgacttct tcctcggcgg ccgcatgcgc ctcgaccgcg
      481 acgccgcgca ccgcgcgatc gacgagcacg tcgcgcggcc gctcgggctc gatccagtcg
      541 atgccgcctg gggcatccac cgggtcgtca acgagaacat ggcggcggcc gcgcgcatcc
      601 acgggatcga gcgcgggcgc gacctgcgcc agtacccgct cttcgccttc ggcggagccg
      661 gccccgtgca ctgctggcag gtggcgcaga tcctgagaat gccgaggatc ctgctgccgt
      721 tcggcgcggg cgcgatgtcg gcgtatggcc tgctcgccgc gccgctcgcc ttcgacttcg
      781 tgcgcacgcg gcgccagcac ctcgacgctg ccgactggac ggcagtcaac gcgctcttcg
      841 gcgagatgga ggccgaaggg cgcgccgtgc tcggacgcgc gggcgtggcg gaggccgacg
      901 tccgcgtgag ccgcgtcgcg gagatgcgct atctcgggca ggggcacgag gtcgaggcga
      961 cgctgccgcg gggcgacctg ggtccggcga gcctgccggc gatccgcgag gccttcgagg
     1021 tcgcctaccg ggcgctctac caccgcctgc cgcagggcgt accgatcgag gccctcaact
     1081 ggcgggtcac cgtcgctggg ccccgtcccg aggtcgtctt ccgcccgtcc ggagcgctgt
     1141 cggccccggc gccagccggc ggggcgacgt ggcacagcgc cgtgaagacc caccgtcggg
     1201 cctggttcgc cgaggcggca ggcttcatcg agacgcccgt gtacgatcgc tacgcgctcg
     1261 gagccggagc gacgttcgag ggcccggcca tcgtcgagga gcgcgagtcg accgccgtca
     1321 tcggccccgg tgggcgctgc cgcgtggacg acggtcttgt catcatcgcg gagctgccgt
     1381 gacgggcgga cgtccggcgc cgaggctcga gaggagacga gccgcgtgat cggcgtcgtc
     1441 ggcgacttca atccggccaa ccctacccac ggcttcacca acgccgccct ggagcatctc
     1501 ggactctcgt tcgaatggcg ggccactgac gccgtggggg tccatccgga gcagtcgctc
     1561 gagccctacg acggcctctg gatcgcgccc gcgagcccct accggagcat ggacggggcg
     1621 ctccaggcca tccgctacgc gcgggagcgg ggtgtgccgc tggtcggtac ctgagggggc
     1681 ttccagcacg cgatcgtcga gttcgcgcgc aacgttctcg gggtccgaga cgccgatcac
     1741 gcagagacga gcccggaggc tccgcggctg gtcgtcacgg cactggcgtg ctccctcgtc
     1801 ggccagcaac acccggtcgc catccgcccc ggcaccgtcg cggccaggct gtacggcgtc
     1861 gccgagtccg tcgaggacta ctactgcaac tacggtgtga accgcgcata cgagcagctc
     1921 ctcgaggacg gcgggctccg gatcagtggc gtgggcgtcg agggcgaggt ccggatcgtc
     1981 gagatccccg agcacccctt cttcgtggcg accctgttcc tgccccaggc gcgctccgcc
     2041 ccggcggcac cgcacccgct cctggccggc ttcgcggccg cgaccgcctc ccgccacgcc
     2101 gagcgagtgc agagaccgtg acgtggatat gatggatccg gtcacgctcg agatcctctg
     2161 gagccgtctc atcggcgtcg ccaacgagca ggcgacggcg ctgcaacgca cttcgttcac
     2221 ctccatcgtg cgcgaggccg gcgacctctc ggccggcgtc ttcgaccgtc gcggcttcat
     2281 gatcgcccag gcggtgaccg gcacgccggg ccacatcaac tccatggcgc tggcgatgaa
     2341 gcacgtgctc gccgagcacc ccgccgacac gctgcggccc ggggacgtgg tgatcaccaa
     2401 cgatccgtgg aagacgtcgg gccacctgaa cgacgtgacc atcgcctcgc cggtgttccg
     2461 tagcgacgac tgcgtggcct tcttcgcctc gacctgccac accgcggaca tcggcggcca
     2521 tgtgttctcg gccgaggcgc gcgaggtcta cgaggagggc ctgttcatcc cgatcatgaa
     2581 gctctacgag gccgggcggg ccaacgcgtc gctgatcgcg ctgatccggg ccaacgtccg
     2641 gctccccgag atggtgctcg gcgacttcca cgcccagatc gccggcggcg ccgtgggcgg
     2701 cgagcggctg ctcgagttca tggacgagtt cgggctcgag cgcctggagc cgctgggcga
     2761 cgagatcatc gcacgcaccg agcgggccat gcgcgaggcc atccgcgcgc tccggcccgg
     2821 tgtgtacgag cacgccatca cgtccgacgg cttcgacgac ccgatcacga tcaaggcgcg
     2881 ctgcgaggtg cggggcgacg agctcacgat cgactacgcc ggatcgtcgc cggccagccc
     2941 tcgcggcatc aacgtggtca tgaactacac ggaggcctac accacctacg gcgtcaaggt
     3001 gatcgtgagc cccgacgtgc cgaacaacga gggcgccttc cgcccgctgc gcatcgccgc
     3061 gcccgagggc tcgatcctca acgtgtgcca ccccgccgcc gtggccgccc gccacgtcat
     3121 cggccacttc ctgccccacg tggtggccgg cgcgctcggt caggcgttgc ccgaccgcgt
     3181 catggccgag gggtccgcca acatctgggg cgtccagatc gccggccgtg acctcgaggg
     3241 ccggccgttc acccacgtct tcttctcgtc cggcggcacc ggcgcccgcc cagggaagga
     3301 cgggctcgac gccaccgcct tcccctcggg cgtgctgggc acgccggccg aggtgctcga
     3361 gagcatcgcc ccggtcctgg tcgagcggaa ggcgctgcgt caggggtcgg gtggagaggg
     3421 ccggtaccgc ggcgggctcg gtcagacgat cgcgttccgg gtgcgaacgc gcgaaccctt
     3481 cacgtgctcc gtcctctgcg accggacacg gcatcccgcg cgcggcttcc tcggcggcgg
     3541 cccgggcgcg gtcggcgagg tgctgatcga cggcgtgcca ccggacaacc cgaaggccga
     3601 gcagctcgtg acgcccggag cgctcgtgga ggtgcggctg ccagggggcg gcggctacgg
     3661 cccgccggcc gagcgcgacc ccgatttgct ggagcgcgac cgtctcgagg gctacgtgtg
     3721 atcggttaac ggcgtctggt cgctagtctc ggcgccaaac ttcgccgacc gtaagccggt
     3781 ccggggtcgg gggtgcgtgg gggcccgggt ccggtgacct gttccccgca gacggaatcc
     3841 gctccacacc cggcgatgga aacagcgcca gaacggcggc gcgcaacgca tcaggggtcg
     3901 ccggacacgc gcccccacgc acccccgacc ccggaccggc cagcatcgtc gtcagcgagg
     3961 ttctgaccgc tagagccggc cgtactcctt gagccgacga tagatcgtcc gccgggagat
     4021 gccgagcgca cgcgccgcct cttccttgtc gttgttgtgg acggccaggg ccctcaggat
     4081 caggtccttc tcgacctcgg ccagcgtggg gacgctcttg ctctccagcg cgctcggcac
     4141 cgccaccgcg gccgtgcggg cggccaggct cggcaggtcg gcgagcgtca gctgctcgcg
     4201 cgagccgagg gcgaacgcgc gctcgatgag gttctccagc tcgcggacgt tgcccgggaa
     4261 gtcgtaggcg gtcagcgccg acagcgcgtc gggggtgatg ccgcggacgt cgcggtggaa
     4321 gcgcttgttg aagcgccgga tgaagtggtt cacgagcggc gggatgtcgt cgcggcgctc
     4381 gcgcagcggc ggcagcacga tacggatgac gttcagccgg tagaagaggt cctggcggaa
     4441 cgtgccggcg tgcatcgcct gctcgaggtt ccggttggtg gcggcgatga ctcgcacgtc
     4501 caccgggaag gccttggtgg accccaccgg gcgaacctgc atctcctgca gcacgcggag
     4561 gagcttgacc tgtagctccg gcggcagctc ggcgatctcg tccaggaaga tcgtgccctc
     4621 gttcgcgccg cggaagagac cgagcgcgtc ggccacggcg cccgagaagg cgccgcggac
     4681 gtggccgaag aactcggact cgagcaggtc gcgcgggatc gcgctgcagt tgacggggat
     4741 gaacggtccc ttggcgcgcg acgacaggcg gtggatggcg gccgcgacca gctccttgcc
     4801 ggtgccgctc tcgccctcga cgagcaccgg cgagtccgtg acggcgacct tgccgatgat
     4861 ctccttcgtc cgctgcatca ccggcgagga gccgatcagc tcgttgacgg tcagctcctc
     4921 gcccaggcgt gcccgcagcg aatgcacctc accccgcagg aagcgccgct ccatgagccg
     4981 ctgcatgagc atccggagct catcgaggat caggggcttg gagagatagt cgtatgcgcc
     5041 ttccttgagg gcctcgatgg cggacgagat ggtcgggtac cccgtcatga tgaccacctc
     5101 gatcgactcg tcgcgcttct tgatctcttt gaggaggtcg agaccgctca tgtccggcat
     5161 gcgcacgtcg gtgagggcgg cgtcgaagac ctgagtcttg agggcctcga cggcctgctg
     5221 tgcggtgccc gcggtggtga cgtgatagcc ccactgcgtg agagcttcct gcagcagcat
     5281 caagattgac cgctcgtcgt cgacgatcag gatctgcgcg ggttgcactg tgaaatcaat
     5341 ttatcatggt gggggtgcca acttgacaag cttttttcga cgggtccttg cgctcaaccg
     5401 atcggcatgt tagcgttagg cttgactttt ttgggaggga gagactcgcc gatggctgac
     5461 tccgcgaccc ttcacctcac cgagcagaac ttcgacgagg ccctggtggc caaccaggga
     5521 atgctgatgg tcgacttctg ggcggaatgg tgtggcccgt gccgcgcgat cgcgccagtg
     5581 ctggacgaga tggtgcgggc ctcggccgga agggtcgctc tcgcgaaggt gaacgtggac
     5641 gagaacccgg gtctcgcggc gcggtacggc attcgctcga tcccgacgat cctgttcatc
     5701 aaggacggca aggtcgtgga ccaggtgatc ggcgccgtgc cgaaggcgca gcttcagaag
     5761 aagctcgaca ccctggcctg acccgggcgc cgaggcgccg agcgtatcag tactggtggc
     5821 accgctgtcc cgtcgctttc tcctacccgt gacgagtttc gagcgctcgc ggggcgggga
     5881 aacctcgtcc ccgtctacgc cgagcttccc gccgacctcg acactccgct ctcggcctat
     5941 ctgaggctgc gcccggggcc ctacgccttc ctcctggaat cggttgaagg cggcgagaag
     6001 tgggggcgct actcgttcct cggcaccgac ccgctcatgg tgctcagcgc gaagaacggc
     6061 cggatgacgc tccgccgagc gggcggacgg gtcgtcgagc ggctgcccga cggcaacccg
     6121 ctcgacgcgc tgcgcgggct gctgcgcgag ttcgagccgg tggccgtgcc cgggctccca
     6181 cgcttccagg gcggcgcggt ggggtacttc tcctacgaca tggtccgcca catggagcgg
     6241 ctgcccgcca ccgtccagga cgacctccgt ctggcggacg cgatcttcat gttcaccgac
     6301 accctgctgg tgttcgacaa cctccgccac cgactgatgg tcatcgccaa cgcgcgcatc
     6361 gaggggcgcg acgacgcctc cctggaccag gcctacgacc gcgccgcgct ccggatcggg
     6421 atgacgctcg ccaagctcgg ccgccccgcg cgtccgccgg cgcccctcgc cctggccgcg
     6481 ccggggccgc tggtggatct cggcgaggag ggcttcacgt ccaccatgga cgaggcgacg
     6541 ttcgtggacg ccgtgcgccg cgccaaggaa tacatcgcgg ccggcgacgc ctaccaggtc
     6601 gtcctctcgc gacgcctgga ctgtccgctg ccggccgacc cgttcacgat ctaccgcgcg
     6661 ctgcgcacca tcaatccctc gccctatctg ttcttcctgc gcctgggacg ggcgacggtc
     6721 gtgggctctt caccggaggt gctcgtgcgc ctcgaaggcg accgggtgga ggagcggccg
     6781 atcgccggca cgtttccgcg cggcgccacc gaagcgcagg acaaggccat cgaggaggcg
     6841 ctgctcgccg accccaagga gcgcgccgag cacgtgatgc tggtggacct tggccgcaac
     6901 gacgtcgggc gggtgacgac gctcggctcc gtgaaggtca cggagttcat gggcgtcgag
     6961 cgctactcgc acgtcatgca cctggtgagc cacgtggtcg gcacgctcag gccgggcgcc
     7021 gacgcgatcg acgtgctgca ggcctgcttc cccgcgggca cgctcaccgg cgcgccgaag
     7081 atccgggcga tggagatcat cgacgagctg gagccgacgc agcgcgggcc gtacggcggc
     7141 gccgtcggct acatctcgta ctcggggaac ctcgactcct gcatcacgat ccgcaccgtc
     7201 gtctgccacg gcaaccgcgc gtcgatccag gtgggcgcgg ggatcgtggc cgactccgac
     7261 ccgaagaccg agtggctgga gacctcgtcc aaggctcggg ggctcgtgct ggccctgcgc
     7321 atggccgcgg cgacggcacg cctgtgatac tcgtcatcga caattacgac tcgttcacct
     7381 acaacctcgt ccagtacctc ggcgagctgg gggccgagct caaggtcgcg cgcaacgacg
     7441 agctcgacgt ggccgccgtc gccgcgctgg ggcccgagcg catcgtcatc tcaccggggc
     7501 cgggcacacc cgacgacgcc ggcgtctcga tcgaggtcat ccggcagctg ggggggacca
     7561 tccccatcct cggcgtgtgc ctgggtcacc aggcgatcgg gcaggccttc ggcgcgaccg
     7621 tggcccgcgc gcaccggcag atgcacggca aggtctcctc gatcgcccac gacgacgcgg
     7681 gcgtcttccg cggcctcgac aaccccttcg tggcgacgcg ctaccattcg ctggcgatcg
     7741 tgcggtcgac ggtccccgcc gacctcgagg tgagcgcgtg gtccgaggac gacggcgaga
     7801 tcatgggcgt ccgccaccgc cgccatccga tcgagggcgt ccagttccac ccggaatcga
     7861 tcctcaccct cgagggcaag aagctcctgc ggaacttcct caagagatga cgcgggggga
     7921 accgcccgag cggctgacgg aagcctcgct gatccgcgcc gtccgccgcc gggtagaggg
     7981 cggatccggc gtgcaggtcg gcatcggcga cgactgcgcc gtgctcgagc ccacgcccgg
     8041 gacccggctg atcgccacca ccgacctgct gctggaggac gtccacttcc gccgccgcta
     8101 cgcggagccc gccgacgtcg ggtggaaggc gctcgcggtg aacctctcgg acgtcgcctc
     8161 gatgggagcc cgtccccgct gggcgctcgt cgcgctcgcc tgtccggcct ccaccacgct
     8221 cgacgaggtc gaggccttct acgcgggctg cctcgccctc gccgccgagc acggcgtggc
     8281 cgtggtgggg ggggacacgt ccgcctcgcc cgctggctgg ctcgtcaaca tcaccgtgct
     8341 cggcgaggcg acccgcgcgc cgctgttgcg ctcgggcgcc cgcgccggcg acatcgtggc
     8401 ggtgacgggc gggctcgggc gctctgcggc cggcctcgca gtcctcgagc gcgccacggc
     8461 cccgccgggc gtcggcgccg aggccttggc cgaggtgacc gccgcgcacc tccgaccccg
     8521 accgcgcgtc gccgagggcc agtggctggc cggggtgggc ggcgtcaccg cgatgatgga
     8581 cctctccgac ggcctcgcca ccgacctcgc gcacatcgcg gaagagagcg gcgccggcgc
     8641 acgcgtggag ctggagcgcg tgcccgtcgc gccggccgcc cgtctggtgg ccgagcggat
     8701 cggcgccgac gcgctcgcct gggccacggg cggcggggag gactacgagc tgctggtcgt
     8761 ctgcgacccg gaggccttcc cacgccttca cgagggatcc ccgcgggcta cgggcacggc
     8821 gctcaccagg gtgggagaga tgaccgccgg caccggcatc acgttcatgg acgccgccgg
     8881 tcgcgagcgt gccgtcgccg cgggcttcga gcacttcgtc accggacacc gcggcgcccg
     8941 ggaccaccgc cggtgaacga ctccgtcgtc ctcgcgctcc tgatcctgct cctgctggtc
     9001 gtgctctcgg cgctgctcaa cggggccgag gcggcctact tctcgctcgg ccgcgcccgg
     9061 ctgcggcggc tccgcggcca gcagccgccc gagcccggcc gcgtcccgct gatcgagcgg
     9121 cctcacgacc tgctcgtgac gctcctggtg ggcatcacgc tgaccaacat cgcggcctcg
     9181 gcgctcgcgg cagcggtgac cgagcggatc gtgggtcccg gctatgccgt gctgatcgag
     9241 accgtcgtga tggtcctggt cctcacgacg ttcacggaag tgctccccat gacggtggcg
     9301 gtgaagcgtc ccgagctcgt cctcgccgtc ggtggccgtc tcgtggcctg gctggcgctg
     9361 ctgctggcgc cggtgcgcgt cgtcctcggc gcgctcagcg ccgtcaccgt acgcctcatc
     9421 ggcggcgagc gacggcccag caaggagctg agcgaggagg agctgcggac gctcgtcgac
     9481 gtcggcgcga gcgagggcgt cgtggagcgc gaggagcgcg agatgatcca caaggtcttc
     9541 gagctggagg acacgctcgt acgctccatc atggtgccgc ggacggacat gttctgcctc
     9601 gacgtggcca cccccgtcga ggccatcctg cccgcgctcc gcgagaacct ccactctcgt
     9661 gtgcccgtgt acgagggatc catcgacgtc atcgtggggg tcctctacac gaaggacctc
     9721 ctaccttacg tcgacggctt gccgcccggg ttcgacctgc gcgcgcacat ccacccgccc
     9781 tacttcgtgc cggaatccaa gcgcgccgac gtgctgctcc aggagctgca ggccaagcac
     9841 ctgcacatgg ccatcgtcgt ggacgagtac gggggcaccg ccggcctcgt atcgctggaa
     9901 gacctcatcg aggagctggt gggggagatc gccgacgagt acgatgagcc cgagcggctc
     9961 atccagcggc tcgacgccac cacctaccgg gtcgccggca agctcccgat cgacgagctg
    10021 aacgccgtga ccggcctcgc catctcgaac gagggctacg ataccgtcgg cgggtgggtg
    10081 ctcgacctgt tcgggcgggt gccacgcaag gcagagcgcc tggagatgcc cgggctggcg
    10141 gtgacggtgg agaaggtgga gcggacccgc gtggtggagg tcctcgtctc cctgcgcaag
    10201 cccgtgggcg gcgaggcggc ggcatgaccc tcctctggat tgccgtcttc tgcgtggtcg
    10261 ccacgatgtt cttctcgatg gcggagatgg ccttcatcgc ggccaaccgt ctcaagctgc
    10321 gtcatctggc cgcggaaggc gaccgcacgg cagcgctcta cctcgaggcc ttccagcgcc
    10381 ccgagcggac cctggccacg gcgaagatgg gggtcccgat cgcccacgtc gtcgcctcgt
    10441 cggcggcaac cttcgcgctg ctacccgttc tcggtggcat cgcgccgctc gtggtgacgg
    10501 cggtcctgac gccgatcatg ctgatcgtcg gcgagatcat ccccaaggcg gtggcccggg
    10561 agtgggccac gaccctcatc ctgtggctct accgcccgct caccgccgcc gcggtcgtcc
    10621 tggccccctt cgtcgtcttc tcgaacttcg cggtcaccgc cctcctccgg ctggccggcg
    10681 tgcgccaggc ggaagcgcgg cagttcgtgt cgcgagagga gctgaaggcc ctgctggccc
    10741 tcgagcccgg cgaggcggac gtgaccacgc aggaggccga gctgatcggg aagatcttcg
    10801 acctgggcga caccaccgtc cgcgagatca tggtgccgct cgtcgacgtc gtgctgctgc
    10861 cggagacggc gacgccacgc gacgccatcg ccctcatccg cgagcgcggc ttctcccgga
    10921 tcccggtcta tcggctgcgc gagagcaacg tggtgggcgt cgtcagcgcc atggacctgc
    10981 tgcggcgggg ggcaggggcc acgcggctgg acgagctgat gcgcgagccg tactatgtgc
    11041 ccgagaccaa gcggatcgac gacctgctgc gcgagatgca gcgcacgcgc gtccagctgg
    11101 ccgtcgtggt ggacgagtac ggcggagcca cgggcctcgt gacgctcgag gacatcatcg
    11161 agcagatcgt cggggagatc cgtgacgagc acgaccggga gccgacgtct gtggagcggc
    11221 tgcgcgacgg ctcgtactgg gtggccgcgc gcgtgaacat cgacgagatc aacgagacgc
    11281 tcgactggac gctgcccaag cacgactacg agaccgtggc cggcctggtg ctcgcgcagc
    11341 tcgggcggat cccgaggcgg ggcgagcagt tccaggtggc cggctacacc atcaccgtgc
    11401 tcgaggcgga cgcccggcgt gtgggggcgg tgaaggtcga gccgccaagg ccggcgcgcg
    11461 tcgagccccg gtagcgttca cctgcaccgc tgaccctcat tccaaggagg tcttcatggc
    11521 tggcaagatg gtcgattttc cctcgaacgg cggcaagacg gcggcctacc tggccacacc
    11581 cccgagcggc aagggacccg gcgtgctcgt gatccaggag tggtggggac tcgtcggcca
    11641 catcaagcag gtgtgcgacc gcttcgccac cgaggggttc acggcgctgg cgccagacat
    11701 gtaccacggc cagaccgcca gcgagcccga cggggcaggc aagctcttca tggccctcaa
    11761 catcgcgcag gccgagaagg atctgcgcgg cgccgtcgac tacctggcgc agcaggcctc
    11821 gaccaagaag gtcggcgccg tcggcttctg catgggaggc cagctcgcgc tgttcgccgg
    11881 caccgtcaac ccgaaggtcg gcgccgtcgt cgacttctac ggcatccacc cgaacgtgaa
    11941 gccggactac accaagctgc aggggcccgt gctcggtctc ttcgccgaga aggacacgtt
    12001 cgtgacgccg cagaccgcgc gcgacgtcga cgcggccatc aagaaggcgg gcaagcagtg
    12061 cgagatccac atctacccga acgcagacca cgcgttcttc aacgacgagc ggccggacgc
    12121 ctacgacaag gctgccgcgg aggatgcctg gcggcgcacg ctcggcttct tccgcaaaca
    12181 cctggcctga ggccgttcct cgaagagacg gccgccagca cccgtgatcc tgtgctcgga
    12241 accggaggcc gtggaggacc cgcgcgggcg ccggaagtca gccgcggggc agctccacca
    12301 gcaggaactt gccgtcgccg cgcagcaccc gcgtgccgtc gagcaggaag gcgcggagcc
    12361 ggtccaggcg ggcgcggttc tcggcctccg gcaggcggtc gtccaccacg gaggagcgcg
    12421 cgtagtcgag gagcgcgtcg tgacggacga agacgtgggt gatgccggct gcggccgcgc
    12481 gagcccgcac ctcgcgggcg tcgcgcgact cgagcaccca gcgccggatc gtgtggtcct
    12541 cgaagaggta gtcgccgaca aacggtcgct cgaggtgata ggcgtcgcgc cgcacgttga
    12601 tcaaccagac ggtcgagccc gccggcagcc cgtcgttgat cagccggtag tacgggtaat
    12661 agtccagtcg ccgcgagagg tacgcctcgc gcggctcggc gccgagggcc gccaggaagg
    12721 gcgcgtcgcc gaagaaccac g
//
LOCUS       DATIAZ010000267         4966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_469136, whole genome shotgun sequence.
ACCESSION   DATIAZ010000267 DATIAZ010000000
VERSION     DATIAZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469136"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VLF19_09590"
     CDS             complement(<1..1680)
                     /locus_tag="VLF19_09590"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSE93546.1"
                     /translation="MSGGAQPTPGVSLPFGATVTILFSDIRGFTEYTEQYGDEAAYRV
                     LRQHSALVRKEIEAFGGRVVKTQGDSFMVYFTTARAAILCAIGIQRSIGDETVAQQDT
                     RIAIGIGINTGEPIQEGGDFFGSTVNLAARICAVAGPGHILISETTRYVAGRIEAVDY
                     VDHGLRELKGFQEPQRVFEVNWGASAARVAVGGQASRELRAELGALAERFSRLGAEML
                     DTSHAMRQAGPPPGDGLAHQLVALSAAFGDFRGRLLQRAAALGVETGAPEGIATLVDA
                     EPILAAIQEAESRSLEAAPAGAAMGDKHTLEVAVQRALSVLTRVLLIGHRDDPVFHPL
                     LECQGRASDLRLKLSRALAGESEYNAQRVDEAIQPFSDLLALVVGRENLDDEKFSRIE
                     DSATRAFGRPLALAGTRGRLFVQGVEAPARPAPTAAAPPAPAASAIAATPTAPAPPAR
                     AEERTEPARAAHAPESRAPAPEPAEMVPPADPRGAAVPWWAAASAAWRAWKTSGVATA
                     HALRAAVAKHPYLLAVPVTRSAEYDEGRLAADYFLLLEHVENVSPAFVRTAL"
     gene            complement(1825..4044)
                     /locus_tag="VLF19_09595"
     CDS             complement(1825..4044)
                     /locus_tag="VLF19_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008018001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSE93547.1"
                     /translation="MATLGTTRREFLIGTGTLVVSFSLAPDLAGDAVASMHGKPKPVA
                     LDQVDSFLAVHKDGTVTMYTGKVDIGTGIRVALPQMVAEELDVALDKVKLVEGDTALT
                     PDQGPTWGSLSVQIAGVQLRQAAATARQALVQMAVQRLGMPSADLAVKDGVIFLRADP
                     SRRVSYAELIGDKEFNLKVDKDAPLKNPRDHTLVGQPVPRPDVPGKVTGAHTYMHDFR
                     VPGMLHARVVRPPAVGAQLLHVDESSIKNVKGARVVRQGSFLAVVADGEWAAVKGARA
                     LKATWSTWEGLPEMSRLWEHVRATPVARDDVAVDRGDPKAAIAGAARKLKATYDFAVH
                     THGSMGPACAVADVKGGKAEIWSPSQAPHWLRREVAHVLQMDPKDIHIQYLDGAGCYG
                     RNGHEDATCDAALISKLVGKPVRVQWMREDEHGWDPKGPPTLVEISAGLDAQGNIVGW
                     DSELWVPKADITEWPRTLAATAAGIPQKEAINPGNIHRNLDPSYPFAHQRAVAHRLDS
                     TPFRPSWIRTPGRMQNTYANEVFMDECAAAAGVDPVEYRLRYLTDLRGIATLRAAANL
                     AKWDTRPSPRRDQSGNIARGRGIAYVKYENVRTYCAGVAEVEVNKQTGAIRCTRFYVA
                     HDCGQIINPDGVRAQIEGNIIQTVSRTLKEELKWNRARVTSVDWATYPILRFPEVPEV
                     VMELINRPNDAPWGVGEPAACLPPPAISNAVFDAVGVRLRSVPYTPDRVKAASRASV"
     gene            complement(4046..4516)
                     /locus_tag="VLF19_09600"
     CDS             complement(4046..4516)
                     /locus_tag="VLF19_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSE93548.1"
                     /translation="MAILNVNGKSHQVDVEADTPLLYVLRDNLGLHGPKFGCGLAQCG
                     SCTVLIDGKAVRSCTFAVSKVGVGKVVTLEGLGTPQRPSPVQQAFIDEQAAQCGYCIN
                     GMVMQATAFLNARSTNPSERDIRQALQDNLCRCGTHQRIIAAVKRAAALMGKGA"
     gene            complement(4643..>4966)
                     /locus_tag="VLF19_09605"
     CDS             complement(4643..>4966)
                     /locus_tag="VLF19_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HSE93549.1"
                     /translation="VGHPAVAESGVIGVPHPVKGEAVVCFVVLRPGQTPSEPLRVELS
                     DRVAQAMGKALKPERVLFARDLPKTRSAKIMRRVIRATYLGREPGDVSSLENPDAVKA
                     VAEAT"
BASE COUNT          647 a         1756 c         1783 g          780 t
ORIGIN      
        1 gagggctgtc cggacgaagg ccggcgacac gttctcgacg tgctcgagca acaggaagta
       61 gtcggcggcg agccgcccct cgtcgtactc ggcgctgcgc gtgacgggca cggcgagcag
      121 atacgggtgc ttggcgacgg ccgcgcgcag cgcgtgtgcg gtggcgacac ccgaggtctt
      181 ccacgcccgc cacgctgcgc tcgccgccgc ccaccacggc accgcggcgc cgcgcgggtc
      241 ggcgggcggt accatctccg ccggctcggg tgccggcgcc cgtgactcgg gagcgtgcgc
      301 ggcgcgggcc ggctccgtgc gctcctcggc gcgcgcgggc ggcgccggag ccgtcggcgt
      361 agcagcgatg gcactcgccg ccggcgccgg cggtgcggca gccgtcggcg ccgggcgcgc
      421 cggggcttcg acgccctgga cgaagagccg gccgcgggtg ccggccagcg ccaggggccg
      481 gccgaacgcg cgtgtcgcgc tgtcctcgat cctgctgaac ttctcgtcgt cgaggttctc
      541 gcgcccgacg acgagggcca ggaggtcgga gaagggctgg atggcctcgt ccacccgctg
      601 ggcgttgtac tcggactcac cggccagggc gcgcgagagc ttgagcctga gatcgctcgc
      661 cctgccctgg cactcgagta acggatggaa caccggatcg tcgcgatgcc cgatgagaag
      721 cacgcgggtc agcaccgaca gcgcccgctg cacggcgacc tcgagcgtgt gcttgtcgcc
      781 catggccgcg ccagctggcg ccgcctccag gctccggctt tccgcctcct ggatggcagc
      841 gaggatgggc tccgcgtcca ccagcgtggc gatgccctcg ggcgcgccgg tctcgacgcc
      901 aagcgcggcc gcgcgctgga gcaaccttcc ccggaagtcg ccgaacgccg cgctcagcgc
      961 cacgagctgg tgcgcgagcc catcgcccgg cggcggcccg gcctggcgca tggcgtggct
     1021 cgtgtcgagc atctccgcgc cgagccggct gaagcgctcg gccagcgcgc ccagctccgc
     1081 gcgcagctcg cggctggcct gcccgcccac ggccacccgc gcggcggacg ccccccagtt
     1141 gacctcgaag acccgctggg gctcctggaa gcccttgagc tcgcgcagcc cgtgatccac
     1201 gtaatcgacg gcctcgatgc ggccggcgac gtagcgcgtc gtctccgaga tcagaatgtg
     1261 cccggggccc gccacggcgc agatgcgcgc ggccaggttc acggtgctgc cgaagaagtc
     1321 tccgccttcc tggatgggct cgccggtgtt gatgccgata ccgatggcga tgcgggtgtc
     1381 ctgctgggcg accgtctcgt cgccgatcga gcgctggatg ccgatggcgc agaggatggc
     1441 cgcgcgggcg gtcgtgaagt agaccatgaa gctgtcgccc tgggtcttga ccacccggcc
     1501 gccgaaggcc tcgatttcct tgcggacgag ggcgctgtgc tggcggagga cccggtaggc
     1561 cgcctcgtca ccgtactgct cggtgtactc ggtgaaccct cggatatcac tgaagagaat
     1621 ggtgactgtg gcgccgaacg gcagcgacac cccgggcgtt ggctgggccc ccccagacac
     1681 cttttatcct ccacacgcgc gggctggcga tgtcacggcg actcgtagag gatagcatcc
     1741 ggagcctcga atccccgtgt cccgacgcgg gcttgtgcgg tcgcccgggg ggagggcgat
     1801 cctgcccccc gggcgggtct gtcatcacac cgaggctcgg ctggcggcct tgacgcggtc
     1861 cggtgtgtag ggcaccgagc gcagcctgac cccgacggcg tcgaagacgg cgttggagat
     1921 ggcggggggc ggcaggcacg cggccggctc gcctacgccc cacggcgcgt cgttgggtcg
     1981 gttgatcagc tccatcacga cctcgggcac ctccgggaac cgcaagatcg ggtaggtcgc
     2041 ccagtccacg ctggtcacgc gcgcgcggtt ccacttcagc tcttccttga gcgtccgcga
     2101 aaccgtctgg atgatgttgc cctcgatctg ggcgcgcacc ccgtccgggt tgatgatctg
     2161 gccgcagtcg tgggcgacgt agaagcgcgt gcagcggatc gcccccgtct gcttgttgac
     2221 ttcgacctcc gccaccccgg cgcagtaggt gcggacgttc tcgtacttta cgtaggcgat
     2281 gccgcgaccc cgggcgatgt tgccggactg atcgcgccgg ggcgagggcc gcgtgtccca
     2341 cttggcgagg ttggccgccg cgcgcagcgt ggcgatgccg cgcaggtcgg tcaggtagcg
     2401 cagccggtac tccaccgggt cgaccccggc cgccgccgca cactcgtcca tgaacacctc
     2461 gttcgcgtac gtgttctgca tgcgacccgg ggtccggatc cacgacggcc ggaagggtgt
     2521 ggagtcgagc cggtgcgcga ccgcccgctg gtgcgcgaac gggtacgacg ggtccaggtt
     2581 gcggtggatg ttgcccgggt tgatggcctc cttctgtgga atcccggccg ccgtcgcggc
     2641 cagggtgcgc ggccactcgg tgatgtcggc cttcggtacc cacagttcgg agtcccaccc
     2701 gacgatgttg ccctgagcgt ccagccccgc cgagatctcg acgagcgtgg gcgggccctt
     2761 cgggtcccag ccgtgctcgt cctcgcgcat ccactgcacg cgcacgggct tgccgacgag
     2821 cttggagatg agcgcggcgt cgcacgtggc gtcctcgtgg ccgttccggc cgtagcagcc
     2881 cgcgccgtcg aggtactgga tgtggatgtc cttcgggtcc atttgcagca cgtgcgccac
     2941 ttctcggcgt agccagtgcg gcgcctgcga gggcgaccag atctccgcct tgccgccctt
     3001 cacgtcggcc acggcgcagg ccgggcccat cgagccgtgg gtgtgcacgg cgaagtcgta
     3061 cgtggccttg agcttcctgg ccgcgccagc gatggctgcc ttcggatcgc cgcggtccac
     3121 cgcgacgtcg tccctggcga ccggcgtggc cctcacgtgc tcccagagcc tggacatctc
     3181 cgggagcccc tcccaggtgg accacgtggc cttcagggcc ctcgccccct ttaccgccgc
     3241 ccactccccg tcggcgacga cggcgaggaa gctgccctgt cggacgaccc gggcgccctt
     3301 gacgttcttg atggacgact cgtcgacgtg gaggagctgc gcgccgacgg ccggtggccg
     3361 caccacgcgc gcgtggagca tgcccggcac ccggaagtcg tgcatgtagg tgtgcgcccc
     3421 ggtcaccttg cccggtacgt ctggccgcgg caccggctgt ccgacgagcg tgtgatctct
     3481 ggggttcttg agcggcgcgt ccttgtcgac cttgaggttg aactccttgt cgccgatcag
     3541 ctcggcgtag gagacgcgcc ggctcggatc ggcccggagg aagatgacgc cgtccttgac
     3601 ggcgagatcc gccgacggca tgccgagcct ctgcaccgcc atctgcacga gcgcctggcg
     3661 cgccgtggcc gcggcctggc ggagctgcac gccggcgatc tgcacggaga ggctgcccca
     3721 cgtgggcccc tggtcgggcg tgagcgcggt gtcgccctcg accagcttca ccttgtcgag
     3781 cgcgacgtcc agctcctcgg cgaccatctg tggcagcgcc acgcggatgc ccgtgccgat
     3841 gtccaccttg ccggtgtaca tcgtcaccgt gccgtccttg tgcacggcga ggaaggaatc
     3901 gacctggtcg agggcgaccg gcttcggttt cccgtgcatg ctggccacgg catcgccggc
     3961 caggtccgga gccaggctga aggagacgac gagggtgccg gtgccgatca ggaactcgcg
     4021 gcgagtcgtc ccgagcgtcg ccatgtcagg ccccctttcc catcagcgcc gcggcgcgct
     4081 tgacggcggc gatgatgcgc tggtgggtcc cgcagcggca gaggttgtcc tgcagcgcct
     4141 gccggatgtc gcgctcggag gggttggtcg accgcgcgtt gaggaaggcc gtggcctgca
     4201 tcaccatgcc gttgatgcaa tagccgcact gcgccgcctg ctcgtcgatg aaggcctgct
     4261 ggaccggcga cggcctctgg ggcgtgccca gaccttcgag cgtgaccacc ttgcccacgc
     4321 cgaccttcga cacggcgaac gtgcacgagc gcaccgcctt gccgtcgatc agcacggtgc
     4381 aggagccgca ctgcgccagc ccgcaaccga acttcgggcc gtgcagcccg aggttgtcgc
     4441 ggaggacgta gagcagcggc gtgtccgcct cgacgtccac ctggtgggac ttgccgttga
     4501 cgttgagtat tgccatacgc cctccctgcg gcggccagcg gattcagccc gcgtcggtcg
     4561 agtgacgatg cgtccggcgg aatggcggga ctgtagtcgg gcagggcgcg gctgtcaatg
     4621 cgcggcgctc cgtgtccggg tgttacgtcg cctcggcgac cgccttcacc gcatcggggt
     4681 tctcgagcga cgacacgtcg cccggctccc ggcccagata ggtcgcgcgg atcacgcggc
     4741 gcatgatctt cgccgagcgc gtctttggaa gatcgcgtgc gaagagcacg cgctcgggct
     4801 tgagcgcctt gcccatcgcc tgggccacgc ggtcgctgag ctcgacgcgc agcggctcgg
     4861 acggtgtctg gcccgggcgc agcacgacga agcagacgac ggcctcgccc ttcaccgggt
     4921 ggggcacgcc gatgacgccg gactccgcga cggccggatg gccgac
//
LOCUS       DATIAZ010000268         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_469715, whole genome shotgun sequence.
ACCESSION   DATIAZ010000268 DATIAZ010000000
VERSION     DATIAZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469715"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VLF19_09610"
     CDS             <1..155
                     /locus_tag="VLF19_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE93550.1"
                     /translation="EPANQFYGDRHGGVKDASGNIWWIATHVEDVPPDEMTRRAEAFM
                     KKPTTG"
     gene            complement(130..861)
                     /locus_tag="VLF19_09615"
     CDS             complement(130..861)
                     /locus_tag="VLF19_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSE93551.1"
                     /translation="MPGIRFDHIAIALPRMADAMGFVVGTLGGAPAFGMDAGVYRFGQ
                     WKFANRARLELLEPLGADGFLHRFLAGRGSGIHHVTFKVPSLAQACARAEARGYEIVG
                     RNERNTYWKEAFLHPRQALGIVIQLAETAPFEGPHRPWSDAPPMPASPPRPVSILGLR
                     MRAGSRERALGQWRGILEADVADLAGALVFTWAGSPMRIAVAIDPCAKDGPLDIELGS
                     DWPVSLPAGPHPVLGVRFSLSSASS"
     gene            1132..2244
                     /locus_tag="VLF19_09620"
     CDS             1132..2244
                     /locus_tag="VLF19_09620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSE93552.1"
                     /translation="MSFASFTLHTDLLRGLDALGFAVPTPIQKDAIPPALEGRDVLAC
                     AMTGSGKTAAFLLPILQRLIDQPRGVTRVLVLAPTRELAAQIDEHRRQLARFTRVGGA
                     AVFGGVAMQPQEQAFRRGADILVATPGRLLDHMRHPYARLAGLEVLVLDEADRMLDMG
                     FLPDIRRIMKALPATRRQTLLFSATLPPAIVTLSREVLLDPVMIDVERPAAPAVGVSH
                     AAYPVAQEAKLPLLLDLLRRPDVRNMLVFTRTKHRANRLADGLVRRGVAADRIHGNRS
                     QAQRTQALAAFKTGACRVLVATDIAARGIDVTGLSHVVNFDVPNLPDDYIHRVGRTAR
                     MEAVGDAVTFVSPAEEADFRAIERAVGARIRRVTTR"
BASE COUNT          359 a          932 c          879 g          360 t
ORIGIN      
        1 tggagccggc gaaccagttc tacggcgacc gtcacggagg cgtgaaggac gcctccggca
       61 acatctggtg gatcgcgacg cacgtcgagg acgtcccgcc cgacgagatg actcgtcggg
      121 cggaggcgtt catgaagaag ccgacgacag gctgaaacgg acgccgagca cggggtgggg
      181 gccggccggc agcgacaccg gccagtcgct ccccagctcg atgtcgagcg gaccgtcctt
      241 cgcgcacggg tcgatcgcga ccgcgatgcg catcggcgag cccgcccacg tgaacacgag
      301 cgcgccggcg aggtcggcga cgtcggcttc caggatgcct cgccactgcc cgagcgcgcg
      361 ttcgcgggag cccgcgcgca tgcggagtcc gaggatcgac acgggccgcg gcgggctcgc
      421 gggcatcggc ggcgcgtcgc tccacggccg gtgcggcccc tcgaagggcg cagtctcggc
      481 gagctggatc acgatgccga gtgcctgccg ggggtgcagg aacgcttcct tccagtaggt
      541 gttccgctcg ttgcggccga cgatctcgta accgcgcgcc tccgcgcgcg cacacgcctg
      601 ggcgaggctc ggcaccttga acgtcacgtg gtggatgccg ctgccgcggc ccgcgagaaa
      661 ccggtgcagg aagccgtcgg ctcccagcgg ctcgagcagc tcgagtcgtg cacggttggc
      721 gaacttccac tggccgaagc ggtagacgcc ggcgtccatg ccgaacgccg gcgcaccgcc
      781 gagggtcccg acgacgaagc ccatcgcgtc cgccatgcgc ggcagcgcga tcgcgatgtg
      841 gtcgaagcgg attccgggca ccggcggtgt agtgtacgcc atcccgggtg gaatcgagct
      901 ccgtgcgccg gcccttcttc tatggctggg tcgtcgtcgc ccgcaaaacc acgaattcct
      961 cgcctttcac cgggcgcccg cgtatactga cgtcgtctgg ttgatcaggg ctcatcggag
     1021 ctcccgcctc tcagaccgcg tttcccggcc acttccgctt cgtggggcgt gttcaagaac
     1081 tggcggaaga ctgaccgggc ccagattgcg ccctcacgag gaactcgttc catgtcgttc
     1141 gcgtcgttca cacttcacac cgatctgctg cgcggcctcg atgccctcgg gtttgccgtg
     1201 cccacgccca tccagaagga cgccatcccc ccggccctcg aggggcgcga cgtgctggcc
     1261 tgcgccatga cgggcagcgg caagaccgcg gcgttcctgc tgccgattct ccagaggctc
     1321 atcgaccagc cgcgtggtgt cacccgcgtg ctcgtgctcg cccccacgcg ggagctcgcc
     1381 gcccagatcg acgagcatcg ccgccagctc gcgcgcttca cccgcgttgg cggcgccgcc
     1441 gtcttcgggg gcgtggccat gcagcctcag gagcaggcgt tccgtcgcgg cgccgacatc
     1501 ctggtggcga cgcccggccg actgctcgat cacatgcggc acccgtacgc ccggctcgcc
     1561 gggctcgagg tgctcgtgct cgacgaggcc gaccggatgc tcgacatggg cttcctgccg
     1621 gacatccggc ggatcatgaa ggcgctgccc gccacgcgcc gccagacgct gctcttctcg
     1681 gccaccctgc cgccggcgat cgtcacgctg tcgcgcgagg tgctgctcga tccggtgatg
     1741 atcgacgtcg agcgccccgc ggcgcccgcc gtgggcgtca gccacgcggc ctatccggtg
     1801 gcgcaggagg ccaagctgcc gctgctgctg gacctgctcc gccggccgga cgtgcggaac
     1861 atgctcgtgt tcacccgcac gaagcaccgg gccaaccgtc tcgccgacgg gctcgtgcgc
     1921 cggggcgtgg cggcggaccg catccacggc aaccgcagcc aggcccagcg gacgcaggcg
     1981 ctggccgcgt tcaagacggg cgcgtgccgg gtcctcgtcg ccaccgacat cgctgcgcgc
     2041 ggcatcgacg tcaccggcct cagccacgtg gtgaacttcg acgtgccgaa cctgccggac
     2101 gactacatcc accgcgtcgg acggacggcg cgcatggagg cggtgggcga cgccgtcacg
     2161 ttcgtgtcgc ccgcggagga ggccgacttc cgcgcgatcg agcgcgcggt cggcgcgcgg
     2221 atccgacgcg tcacgacgcg ctgacctcac agaggccgcc gggctcggct gcaagggcgc
     2281 gctgcgcagc gcctcgaggc gaaagccggt cgcggtggcg tgaacggggc cgcctttgat
     2341 agggtgtgca ggccacgcca cacgtgcctg gaggctcgag catgcgcatc ggcgtctctc
     2401 tcaccagcgc ccaccggacg agcgacccgc gcgccaccgc gcgcttcatg atcgagcgcg
     2461 cggcagcggc ccggcgcgcc gacctcgact cgctcttcct cggcgaccac cacgcgacgg
     2521 accagcccta
//
LOCUS       DATIAZ010000269         4233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1785166, whole genome shotgun sequence.
ACCESSION   DATIAZ010000269 DATIAZ010000000
VERSION     DATIAZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785166"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1601)
                     /locus_tag="VLF19_09625"
     CDS             complement(189..1601)
                     /locus_tag="VLF19_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSE93553.1"
                     /translation="MRGIVGYGAYLPYWKIERKAIGEALGTPPGPGTRAVASYDEDTT
                     SMGVEAARAALRGLAGPAPEAVYFATTDPAYLDKTNATAIHAALDLVSGAAAYDMLGS
                     VRSAAGALRAALDARGPTLAVSSDVRTGLAGGGDERDGGDGAAALVLGDESVGHHVLA
                     EPLAIASTTAEFLERWRLPGEPASRQWEERFGEAAYVPLGESALAEALKQAGVTAPMV
                     TRLIVAGPHGRAVKRLAAAAGFRREAVADDLTSAVGNCGAAHAGLVLADALDRAQPDD
                     VIAVVSLADGADATIWRTTAAVTARRQRPSVADQIASGTGRVAYATWLAWRGFLRREP
                     PRRPDPQPPEAPAAFRHEPWKFAFTGSRCQACATVHLPPQRVCVKCQAIDRMAPERLA
                     ESKARIATFTVDRLAFSPSPPMVVAVIDFDGGGRYPCELTDVDPKALRIGDRLEMTFR
                     RLLTAGGVHNYFWKARPIRN"
     gene            1693..2685
                     /locus_tag="VLF19_09630"
     CDS             1693..2685
                     /locus_tag="VLF19_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE93554.1"
                     /translation="MPARGEAIDIAPGVAWIRMPLPFQLDHINLWLLEDGDGFTLVDT
                     GVGLDDTRAAWERLLAGRMGGRPVTRLIVTHFHPDHMGNAAWLAERFGADVWCTQGEW
                     LSAQYARRTRDQRDLEGRLAHYRRNGAPAESIERLRRRGNHYPGLVPAVPPDYRPVVE
                     GDALTIGGRRWQAIVGRGHSPEHLTLWSAEAGVLISGDQVLPKITTNVSVWPEQKGAN
                     PLRLYLDSLDRFRSVGDRALVLPSHGLPFRGLHERIEQLRVHHAARLGEALDALAEPR
                     SAAEIIPALFRRPLDTHQLGFAMGEALAHLHFLEADGRAVRVVGPDGVHRFRKA"
     gene            complement(2697..3311)
                     /locus_tag="VLF19_09635"
     CDS             complement(2697..3311)
                     /locus_tag="VLF19_09635"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93555.1"
                     /translation="MIRVKQLLGALATVALASAAAQAGPPTDIVKTHVEQALEVLAKQ
                     GEADSAKRRESIRQIAQAAFDFPDMARRTLGQHWRQRSPEERERFTRLFTHLLERAYF
                     SRIDSFHQGSAVKYIGEDVDGPTASVKTVVVTDKGSTIPVEYLTHKVDGRWRIYDVKI
                     ESVSLVSNYRAQFNKVIVSSSFEELLRRLEQRSGALPAVAPTGG"
     gene            3698..4210
                     /locus_tag="VLF19_09640"
     CDS             3698..4210
                     /locus_tag="VLF19_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE93556.1"
                     /translation="MTMKDIVRDDAARGHAIVVQPGEGPSFWQPVPACGHADPTLTPA
                     NTRFAGLSMGYQTIAPGGRVREHSHGDQVELQICFRGRGRVVVDGENHPLVAGTACFL
                     GHEVKHEIVNEGLDDLVMLWVISPPGLEHFFATIGRARRAGEPAPEPFARPADVVAVE
                     RAMGMNDTRA"
BASE COUNT          580 a         1493 c         1551 g          609 t
ORIGIN      
        1 ggagcggagc tggtgccgaa gccggctctg gcgccgcgcg cgtatgctac cggggcagct
       61 tcgccacacg ttcgctgggt ggtgatgccc agccgccccc gttcctatac ctggcggaag
      121 ccggcggatg agcgagcgcc aacgtgctgc tgaaggtgca cacgctgtgg cttcgcgaat
      181 ggttcccgct aattacggat cggtctggcc ttccagaagt agttgtggac gccaccggcg
      241 gtgagcaggc ggcggaacgt catctcgagc cggtcaccga tccgcagggc cttcgggtcg
      301 acgtcggtca gctcgcacgg gtaacgcccg ccgccgtcga agtcgatgac ggcgacgacc
      361 atcggcggcg acggcgagaa ggccaggcgg tccaccgtga aggtcgcgat ccgcgctttc
      421 gactccgcca gtcgctccgg ggccatccgg tcgatggcct ggcatttcac gcagacgcgc
      481 tgcggcggca ggtgcaccgt ggcgcaggcc tggcagcgac tgcccgtgaa cgcgaacttc
      541 catggctcgt gacggaaggc ggcgggcgcc tcggggggct gcgggtcggg gcggcgcgga
      601 ggctcgcggc ggaggaagcc gcgccacgcg agccaggtgg cgtaggccac ccggccggtg
      661 ccgctcgcga tctggtcggc cacgctcggc cgctggcggc gggccgtcac cgccgccgtc
      721 gtccgccaga tcgtcgcgtc cgctccatcg gcgagcgaga cgacggcgat gacgtcgtcg
      781 ggctgcgccc gatcgagcgc gtcggcgagc acgaggcccg cgtgcgcggc gccgcagttg
      841 ccgacggccg acgtgaggtc gtcggcaacc gcctccctcc ggaaacctgc ggcggccgcc
      901 aggcgcttga cggcgcgccc gtgcggaccg gccacgatga gccgcgtcac catgggcgcg
      961 gtgacccccg cctgcttgag ggcctcggcc agcgcgctct cgccgagcgg cacgtaggcg
     1021 gcctcgccga agcgctcctc ccactgtcgc gaggccggct cgcctggcag ccgccaccgc
     1081 tcgaggaact ccgcggtcgt ggacgcgatg gcgagcggct cggccagcac atgatggccg
     1141 acggactcgt cgccgaggac gagggccgcg gcgccatcgc cgccgtcgcg ctcgtcgccg
     1201 ccgccggcca gaccggtgcg cacgtcgctc gagaccgcga gtgtgggccc ccgcgcgtcg
     1261 agcgccgcgc gcagcgcgcc ggcagcggag cggacggacc cgagcatgtc gtaggcggcg
     1321 gccccggaga ccaggtcaag cgccgcgtgg atcgccgtcg cgttggtctt gtcgaggtac
     1381 gccgggtcgg tcgtcgcgaa gtagaccgcc tcgggcgcgg ggcccgccag gccgcgcagc
     1441 gccgcgcgcg cggcctcgac gcccatcgac gtcgtgtcct cgtcgtacga ggcgacggcg
     1501 cgcgtgccgg ggccgggagg ggtgccgagc gcctcgccga tagccttccg ctcgatcttc
     1561 cagtagggca ggtaggctcc gtacccgacg attcctcgca tggcgcgcct atcttacacg
     1621 gccgacgccc cggcggcggt gcggtcatga gttacactgc gcctcatggc tctcgagcat
     1681 ccgtggacgg acgtgcctgc gcgcggcgag gcgatcgaca tcgccccggg cgtggcgtgg
     1741 attcgcatgc cactgccctt tcagctcgac cacatcaact tgtggctcct cgaggacggc
     1801 gacggcttca ccctcgtgga cacgggcgtt gggctcgacg acacgcgcgc ggcgtgggag
     1861 cggctgctcg cgggacggat gggcggccgg cccgtcacac gcctgatcgt cacccacttc
     1921 catcccgacc acatgggcaa cgccgcctgg ctcgccgagc gcttcggcgc cgacgtctgg
     1981 tgcacgcagg gcgagtggct ctccgcgcag tacgcgcggc gcacccgcga ccagcgcgac
     2041 ctcgagggcc ggctcgccca ctaccgccgc aacggcgcgc ccgcagagtc gatcgagcgc
     2101 ctgcgacggc ggggcaacca ctatcccggg ctggtgccgg cggtgcctcc cgactaccgg
     2161 ccggtcgtcg agggcgacgc gctgaccatc ggcggccggc gctggcaggc catcgtcggc
     2221 cgcgggcact cgccggagca cctgacgctc tggagcgcgg aggcgggcgt gctgatctcc
     2281 ggcgatcagg tgctgccgaa gatcacgacg aacgtgagcg tctggccgga gcagaagggt
     2341 gcgaacccgc tgcggctcta cctcgattcg ctggatcgct tccgatcggt gggggaccgg
     2401 gcgctggtcc tgccgtcgca cggcctgccg ttccgcgggc tccacgagcg catcgaacag
     2461 cttcgcgtcc accatgccgc gcgcctgggc gaggcgctcg atgccctcgc cgagccgcgg
     2521 agcgctgcgg agatcatccc tgccctcttc cgccggccgc tcgacacgca ccagctcggc
     2581 ttcgcgatgg gcgaggcgct ggcgcacctg cacttcctcg aggccgacgg ccgcgcggtg
     2641 cgcgtcgtcg ggcccgacgg cgtgcaccgc ttcaggaagg cctgacgccg ggaggactag
     2701 ccgccggtgg gcgccacggc cggcaaggcg cccgaccgct gctcgagccg gcgcagcagc
     2761 tcctcgaacg aggacgagac gatcaccttg ttgaactgcg cccggtagtt cgacaccagg
     2821 ctgacgctct cgatcttcac gtcgtagatg cgccagcggc cgtccacctt gtgcgtgagg
     2881 tactcgacgg gaatcgtcga gcccttgtcg gtgaccacca ccgtcttgac actggccgtg
     2941 ggcccgtcga cgtcctcgcc gatgtacttc accgcgctgc cctggtggaa cgagtcgatg
     3001 cgcgagaagt aggcgcgctc cagcaggtgg gtgaacaggc gcgtgaagcg ctcgcgctcc
     3061 tcgggcgagc gctgccgcca atgctgaccg agcgtgcgtc gggccatgtc gggaaagtcg
     3121 aaggcggcct gggcgatctg gcggatggac tcgcgccgct tggcactgtc ggcctcgccc
     3181 tgcttcgcga gcacctcgag cgcttgctcg acgtgcgtct tgacgatgtc ggtgggcggt
     3241 cccgcctgcg ccgcggcgga ggccagcgcc accgtggcga gcgccccgag gagctgcttc
     3301 acgcggatca tgcttccctc caatctccag tatccgtgcg tcggcgttga cgtgcaaggc
     3361 gtgggccggc gctccccact cgatgcattg gacgggtcca tgaccgccac gcttcacccg
     3421 atccggaaat acagggggtt gtgggtgcgg agatgggtcc ggttcgccgc ggttgcgagc
     3481 gcaacagggg ctgttgacag cgcgtgtggc ctgcttgtca cgcgactggg aggaatacct
     3541 ggtaggtgag aggactgccg gcttgacgag gtgcgccacg gcgcagcggg cgagccgaac
     3601 gttgggggct gggaggccat gtcggggccc gctgttctta gtacactgag cggcacggga
     3661 cgccacgggg ccagtgctcg aagaggggga cgaggccatg accatgaaag acatcgtgcg
     3721 agacgacgcg gcacgaggcc acgcgatcgt ggtgcagccg ggcgagggac cgtcgttctg
     3781 gcaaccggtc cccgcatgcg ggcacgccga tcccacgctc acgccggcca acacgcgctt
     3841 cgccggcctg tcgatgggct accagacgat cgcgccgggc ggtcgcgtgc gggagcactc
     3901 gcacggtgac caggtcgagc tgcagatctg cttccggggc cgcggccgcg tcgtcgtgga
     3961 cggggagaac catccgctcg tggccggcac cgcgtgcttc ctcggccacg aggtcaagca
     4021 tgagatcgtg aacgagggtc tcgacgacct ggtcatgctg tgggtcatct ccccgcccgg
     4081 cctcgagcac ttcttcgcga cgatcggccg cgcccgccga gccggcgagc ccgcgcccga
     4141 gcccttcgcc cgtccggcgg acgtggtcgc cgtcgagcgc gccatgggca tgaacgacac
     4201 gcgggcctga cgcgatgccg accgtctcga tcg
//
LOCUS       DATIAZ010000270         2358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_734698, whole genome shotgun sequence.
ACCESSION   DATIAZ010000270 DATIAZ010000000
VERSION     DATIAZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734698"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VLF19_09645"
     CDS             <1..605
                     /locus_tag="VLF19_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93557.1"
                     /translation="ATWLLVSAAAASSAVAPRDVVQSAVVRVVQAIEESHLARPEPPA
                     KPVGGKLRLEIRRVATELFDFEEIARRALSRHWAGRSSAEQAEFVTLFTDLLERAYFG
                     RIEAYSGEKIVYTGEAVDGDYATVRSRILTRRRTETALDYRMHKIAGRWKVYDVVIDG
                     VSFVSTYRSEFNRIIQSSSWDDLIERLRKKRFTVVERSQT"
     gene            654..2174
                     /locus_tag="VLF19_09650"
     CDS             654..2174
                     /locus_tag="VLF19_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HSE93558.1"
                     /translation="MPAAPGLSWPDYAVLAAALALLVVIGLRLTRGQRDTVDFFLARR
                     RVPWWAVCLSFLATEVSAVTIISVPATAYGENWQYVQFFVGSSAAKLAVAFLFIPAFY
                     RHDVTTIYEFLAHRFGRATQVTGSLFFFLTRLTGSGARLTATAIAVSILVGWRIETTI
                     VVTVAVSIAYIATGGVSAIVWTNVVQALMFLGAGALTLGYLVMQIDGGVDAILRVAGD
                     AGRLQIVNWGPSPGAPDFWTRLASDPNIVWVAVLNGFVGSMAAFGTDHDLMQRLLTVE
                     TRRQSQRTLALTPLGTLLTLAIYLGLGAALYTFYAQHGAPALGRNDEVLPHFVREAMP
                     AVLRGLMLAAIVLASIDSPLGSLSASFVTDIYRPLLVRGRPEAHYLRVSRVAVLGFGA
                     VLGLLAWVFLGWRDQILWLVFKIAGVPFGSLLGVFLLGLLSRRRVADPANVVAMVVMT
                     LVNLTLLLLSETGRIRFPWSWLVIVGTVGTMLIALALTPLVTRTRPERPPSSPPPE"
BASE COUNT          280 a          861 c          796 g          421 t
ORIGIN      
        1 tggcgacgtg gctcctggtc tcggcggccg cggcgtcgtc cgcggtggcg cctcgcgacg
       61 tcgtgcagtc cgcggtcgtc cgggtcgtcc aggccatcga ggagtctcac ctcgcgcggc
      121 ccgagccccc ggccaagccg gtcggcggca agcttcgact cgagatccgc cgcgtcgcga
      181 ccgagctgtt cgacttcgag gagatcgccc gccgggccct ctcgcgccac tgggcgggcc
      241 gctcgtccgc cgagcaggcg gagttcgtca cgctcttcac cgacctcctc gagcgcgcat
      301 acttcgggcg catcgaagcg tattcgggcg agaagatcgt gtacaccggc gaggctgtgg
      361 acggcgacta cgcgaccgtg cgctcgcgca tcctcacgcg gcggcggacg gagaccgccc
      421 tcgactaccg catgcacaag atcgccggcc gctggaaggt gtacgacgtc gtcatcgacg
      481 gggtcagctt cgtctccacg taccggagcg agttcaaccg catcatccag tcgtcctcct
      541 gggacgacct gatcgagcgc ctgcgcaaga agcggttcac cgtcgtcgag cgcagccaga
      601 cctgatccgc cggcgcgcgc gggcggccgt gcgctccggg agtatgatgg gacgtgcccg
      661 ccgcgcccgg cctcagctgg cccgactatg cggtcctcgc cgctgcgctg gcgctgctgg
      721 tcgtcatcgg cctccggctc acccgcgggc agcgggatac cgtcgacttc ttcctcgcgc
      781 gtcgccgggt gccgtggtgg gcagtgtgcc tcagcttcct cgccaccgag gtcagcgcgg
      841 tcacgatcat ctcggtgccg gccacggcct acggcgagaa ctggcagtac gtgcagttct
      901 tcgtcggctc gagcgccgcg aagctcgcgg tggcgttcct cttcatcccc gccttctacc
      961 gccacgacgt cacgacgatc tacgagttcc tcgcccaccg cttcggccgc gccacgcagg
     1021 tgacgggctc gctcttcttc ttcctcacgc gcctgacggg ctccggcgcg cggctgacgg
     1081 ccacggccat cgcggtgtcg atcctggtcg gctggcgcat cgagacgacc atcgtcgtga
     1141 ccgtcgccgt ctcgatcgcc tacatcgcga ccggcggcgt gtccgcgatc gtgtggacga
     1201 acgtcgtaca agcgctgatg ttcctcgggg cgggcgccct cacgctcggg tatctggtga
     1261 tgcagatcga cggcggcgtg gacgcgattc tccgcgtcgc gggcgatgcc ggccggctgc
     1321 agatcgtcaa ctgggggccg tcgccgggcg cgccggactt ctggacacgc ctggcgagcg
     1381 atcccaacat cgtgtgggtc gcggtcctca acgggttcgt cggctccatg gccgccttcg
     1441 gcaccgacca cgacctgatg cagcgcctgc tgacggtcga gacacgacgc cagagccagc
     1501 ggacgctcgc cctcacgccg ctcggcaccc tgctcacact ggccatctac ctgggtctcg
     1561 gcgccgctct ttacacgttc tacgcgcagc acggggcgcc ggcgctcggc cgcaacgacg
     1621 aggtgctgcc ccacttcgtg cgggaggcga tgccggccgt gctgcgcggg ctcatgctcg
     1681 cggcgatcgt gctggcctcg atcgactcgc cgctcggctc gctctccgcc tcgttcgtca
     1741 ccgacatcta ccggccgctg ctcgtccgtg gccggccgga ggctcactac ctgcgcgtct
     1801 cgcgcgtggc ggtgctcggc ttcggcgccg tgctcggcct gctcgcctgg gtgttcctcg
     1861 gctggcgtga tcagatcctg tggctagtct tcaagatcgc cggggtgccg ttcggctcgc
     1921 tcctcggtgt gttcctgctg ggtttgctct cccgccgccg ggtggccgac ccggcgaacg
     1981 tggtcgcgat ggtcgtgatg acgctcgtga acctgacgct gctgctgctc tcggagacgg
     2041 ggcgcatcag gttcccgtgg tcgtggctgg tgatcgtcgg taccgtgggt acgatgctga
     2101 tcgcgctggc gctcacaccg ctcgtgaccc ggacgaggcc cgagcggccc ccttcgagtc
     2161 ctcccccaga ataggattgc gccggcggag ccaacgctcg agccgccgtt tctcctgcag
     2221 cctctcgtga ggccgccttc gcctgctcgg ggacatggcc cggcccgcag gcgatcatca
     2281 ctcggagggg ccgcgccgtt cctgcgcgtc cggcgccacg cttcttgtcg gcgcgatcga
     2341 ggccgcgccg cagcttcg
//
LOCUS       DATIAZ010000271         5627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_207647, whole genome shotgun sequence.
ACCESSION   DATIAZ010000271 DATIAZ010000000
VERSION     DATIAZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207647"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VLF19_09655"
     CDS             <1..409
                     /locus_tag="VLF19_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93559.1"
                     /translation="TVGLAGSERKHPRELSGGMRQRVSLCRALIHEPELLLLDEPFAA
                     LDAFTREEMWELHQRLRQGREFTGVLVTHDLREAVFLARTIHVMSADPGRVAHVHEVD
                     LAFPRALADVYSPVTTELIRLMREQIRPAASAT"
     gene            458..1204
                     /locus_tag="VLF19_09660"
     CDS             458..1204
                     /locus_tag="VLF19_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93560.1"
                     /translation="MMRRVALPAIVFTVLLLVWEVAVPLLDIQQIVLPRPTAIAATVA
                     DKAGPIAFHARHTLVTTMVGFGAAVGLGLLLGFAIGASATVHDALYPLLVGFNSIPKA
                     AVVPLLVMWFGIGAVPAVLTAFLLAFFPVAVNVAVGLATLEAELRDVLRALGASRWQI
                     FVKVGVPRTMPLFFASLKVAVSSAFVGSVISETVASNAGIGYLMAVAASDFNTSLAFG
                     GLFVLAALGILLYGVFAAVERRMTAWAYTE"
     gene            1186..2403
                     /locus_tag="VLF19_09665"
     CDS             1186..2403
                     /locus_tag="VLF19_09665"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HSE93561.1"
                     /translation="MGVHGVSVDLNLVEWVGLAVRWFHLVVAIGWIGASLYFMWLDAS
                     LEPPTAPQERVDGEVWMVHSGGFYHVVKRRLRPGEMPAHLHWFKWEAALTWASGFALL
                     ALVYYANAGAYLVDRTVLTMPPAAATLLGIALLAGSWVVYDLLWRSPLGEGRARAATW
                     LSWGLLFALAWAACRLFGGRGAYIHVGAALGTIMVANVWMIIVPAQRELIAATREGRE
                     PDWTLGARAKRRSTHNSYVTLPVIFLMFSNHYPSTYGHPLNWLVLCLLIVAGAGVRHA
                     MIMAEKRHPARWVWVPVAAAVIAAVALTAPAWRATARPADGPRVPFAVARGIVELRCV
                     SCHSKAPTDDVFRAAPQGLVLEMPETIRERAAQIKERTVTTRTMPFGNKTGMTDEERR
                     LLGRWVDQGARLQ"
     gene            2411..4717
                     /locus_tag="VLF19_09670"
     CDS             2411..4717
                     /locus_tag="VLF19_09670"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE93562.1"
                     /translation="MIGASPRRKEDGRLLVGAGRFVDDVTRPGLLHLGVVRSAEAHAR
                     LVKVSVERARALAGVLAVWTADDLPEVAGGLPAGYGGGYKGRPFTEPVIARDAVRHVG
                     EPLAVVVAEGPARLADAMEAVTVEYEPLPPLADVEGALVSPTRLHEGWPDNSTLPVSG
                     AIGDAGAAMASAEVVVEARIRHPRLSGMAIETRGTLAYVDETDTLVVVASMQNPYLLR
                     DKIATALGLASERVRVVTPDVGGAFGPKGSVYQDHLLVAAAALRLRRPVKHVESRREH
                     LVATGHDREQVHDARLGLRRDGTIVALEDRFLADVGAYPVEGEGLTLNTVNHMPGPYR
                     VPHYRSAGRSVVTNKTQNAAYRGAGRPEAVLVTERLMDLAARRLGLDPAEIRRRNLVR
                     PEQMPYRPGLTYKDGVPVTYDAGDFPAAFERALALIGYDAWRARQRERGRATQRFGIG
                     LACYAQGTGLGPFEGATVRVDPSGKVYVVIGVTGQGQGHATTLAQVCAESLGAPVDDV
                     EIVAGDTSVFPFGMGTGGSRVMANAGPAVARAARSVRARAARVAADVLECAPEDVRIT
                     DGRAHVAGVPDRGVGLGRLAHVAIRSKTLRGAVGEPGLNACVYFQPETVTWAFGTHAA
                     VVEVDAETCATKLVAYCVVHDPGRAINPMIVEGQLQGGAVQGLAAGLMEELVYDASGQ
                     LVTGSLMDYAIPRADDLPAIDVALTEHRSAINELGVKGVGESGAIPGAAAIANAIEDA
                     VADLGVVIGDVPVTPARLFALLAAATHR"
     gene            4825..>5627
                     /locus_tag="VLF19_09675"
     CDS             4825..>5627
                     /locus_tag="VLF19_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE93563.1"
                     /translation="MWRNSAATELAEARELAALLELRARDPAQVSARACYLELVGVTP
                     GSRVLDVGCGSGAVTRDVARRVRPAGRVVGLDPGPGFLAIAAELVEREGLADVVELRL
                     GDARALPFADAEFDVALAVTTLAHVPDGERALPELVRVVRPGGRVGIFDLDGDGAILA
                     HPDRTLTRRIVAASSDHIMVDGWLARRLPALLAASGLEDIGVRAFTPVERDPAGFYAR
                     LAERRAAVAVQAGAITVDEQREWLAALRAEQAAGRFLGGQTHLFVWGTRR"
BASE COUNT          693 a         2057 c         2041 g          836 t
ORIGIN      
        1 cacggtgggg ctcgccgggt ccgagcgcaa gcatccgcgc gagctgtcgg gcggcatgcg
       61 ccagcgcgtg agcctctgcc gcgcgctcat ccacgagccg gagctgctgc tgctcgacga
      121 gcccttcgcc gcgctcgacg ccttcacgcg cgaggagatg tgggagctac accagcggct
      181 ccgccagggc cgcgagttca ccggcgtcct ggtgacccac gacctccgcg aggcggtgtt
      241 cctcgcccgc accatccacg tcatgtccgc cgaccccggg cgcgtggcgc acgtgcacga
      301 ggtggatttg gcgtttccgc gggcgctggc cgacgtgtac agtcccgtca cgaccgagct
      361 gatccggctg atgcgcgagc agatccgccc ggcggcgagc gccacatgat cggtcacgtg
      421 aggagccccg aatgatccgc cgggtgacga gcgcgccatg atgcgccgtg tggcgctgcc
      481 agccatcgtg ttcaccgtgt tgctgctggt ctgggaggtg gcggtgccgc tcctcgacat
      541 ccagcagatc gtgctgccgc ggccgaccgc catcgccgcc accgtggccg acaaggccgg
      601 cccgatcgcc ttccacgcgc gtcacacgct ggtgacgacc atggtcggct tcggcgccgc
      661 tgtcgggctc gggctgctgc tgggcttcgc catcggggcc tcggcgacag tccacgacgc
      721 gctgtacccg ctgctcgtcg gcttcaactc gataccgaag gccgcggtgg tgccgctgct
      781 cgtcatgtgg ttcggcatcg gcgcggtgcc agcggtgctg acggcgttcc tgctcgcctt
      841 cttcccggtc gccgtgaacg tcgccgtggg gctggccacg ctggaagccg agctgcgcga
      901 cgtgctgcgc gccctcgggg cctcccgctg gcagatcttc gtcaaggtcg gcgtgccgcg
      961 cacgatgccg ctcttcttcg cctcgctgaa ggtcgcggtg tcgtcggcct tcgtcggctc
     1021 ggtgatctcg gagaccgtcg cctccaacgc gggcatcggc tacctcatgg ccgtcgccgc
     1081 ctcggacttc aacacgtcgc tggccttcgg cgggctcttc gtcctggccg cgctgggcat
     1141 cctgctctac ggcgtcttcg ccgccgtcga gcgacggatg acggcgtggg cgtacacgga
     1201 gtgagcgtgg acctgaacct cgtggagtgg gtcgggctgg ccgtgcggtg gttccacctc
     1261 gtcgtcgcca tcgggtggat cggtgcgtca ctctacttca tgtggctcga cgcgagcctc
     1321 gagccgccga cggcaccgca ggagcgcgtg gacggcgagg tctggatggt gcactcgggc
     1381 ggcttctacc acgtcgtcaa gcgccggctg cgcccgggcg agatgcccgc ccacctgcac
     1441 tggttcaagt gggaggccgc gctcacgtgg gcgagcgggt tcgccctgct cgcgctcgtc
     1501 tattacgcga acgccggtgc ctacctcgtg gatcggacgg tgctcacgat gccgccggcc
     1561 gccgcgacgc tcctggggat cgcgctcctg gccggctcgt gggtggtcta cgacctgctc
     1621 tggcgctcgc cgctcggcga gggccgggcg cgggcggcga cgtggctctc gtgggggctg
     1681 ctcttcgcgc tggcgtgggc ggcctgccgg ctcttcggcg ggcgcggcgc ctacatccac
     1741 gtgggcgccg cgctgggaac gatcatggtc gccaacgtgt ggatgatcat cgtgcccgcg
     1801 cagcgcgagc tgatcgcggc cacccgcgag gggcgcgagc ccgactggac gctcggcgcg
     1861 cgcgccaagc gccggtccac gcacaacagc tacgtgaccc ttcccgtgat cttcctgatg
     1921 ttcagcaacc actatccgtc cacctatggc catccgctca actggctcgt gctgtgcctg
     1981 ctcatcgtcg ccggagcggg agtgcgccac gcgatgatca tggccgagaa gcgccacccc
     2041 gcgcggtggg tgtgggtgcc cgtcgccgcg gcggtgatcg ccgccgtggc cctcaccgcc
     2101 cccgcgtggc gggccaccgc ccggcccgcc gacggcccgc gcgtgccgtt cgccgtcgcg
     2161 cgaggcatcg tcgagctccg ctgcgtctcg tgccactcga aggcgccgac ggacgacgtt
     2221 ttccgcgcgg cgccgcaggg gctcgtgctc gagatgcccg agacgatccg cgagcgggcg
     2281 gcccagatca aggagcgcac ggtgacgaca cgcacgatgc cgttcgggaa caagacgggc
     2341 atgaccgacg aggagcgccg cctgctcggc cgctgggtcg atcagggcgc gcgactgcag
     2401 tgagcgggcg atgatcggcg cctcgccgcg acgcaaggag gacggccgcc tgctcgtggg
     2461 cgccggccgg ttcgtggacg acgtcacgcg ccccgggctc ctgcacctcg gggtcgtcag
     2521 gagtgccgag gcccacgccc ggctcgtcaa ggtcagcgtg gagcgcgccc gcgcgctcgc
     2581 cggcgtgctc gccgtgtgga ccgcggacga cctgcccgag gtcgccggag ggctgccggc
     2641 cggctacggc ggcgggtaca agggccggcc gttcaccgag cccgtgatcg cgcgcgacgc
     2701 cgtgcggcac gttggcgagc cgctcgccgt ggtggtcgcc gaaggtccgg cgcggctcgc
     2761 cgacgcgatg gaggcggtga cggtcgagta cgagccgctg ccgccactcg ccgacgtgga
     2821 gggcgcgctc gtctcgccca cgcgcctgca cgaaggctgg ccggacaact ccacactgcc
     2881 ggtgagcggg gccatcggcg acgccggtgc ggcgatggcc tcggcggagg tggtggtcga
     2941 ggcccgcatc cgccacccgc gcctctccgg catggcgatc gagacgcgcg gcacgctcgc
     3001 ctacgtggac gagacggaca ccctggtcgt cgtcgcctcg atgcagaacc cttatctcct
     3061 gcgcgacaag atcgccacgg ccctcgggct ggcgtcggag cgcgtacggg tcgtcacacc
     3121 cgacgtcggc ggcgccttcg ggcccaaggg ctccgtgtac caggaccacc ttctcgtcgc
     3181 ggccgccgcg ctgaggctcc gccggccggt caagcatgtc gagagccgcc gcgagcatct
     3241 cgtcgccacc gggcacgacc gcgagcaggt ccacgacgcg cgcctcggcc tccgccgcga
     3301 cggcacgatc gtggcgctcg aggaccgctt cctggccgac gtcggcgcct atccggtcga
     3361 gggcgagggc ctcacgctca acaccgtcaa tcacatgccc ggtccgtatc gcgtgccaca
     3421 ctaccggagc gccggccgga gcgtggtgac gaacaagacg cagaacgcgg cctatcgcgg
     3481 tgccggccgg cccgaggccg tcctcgtcac ggagcgcctg atggacctcg ccgcgcgccg
     3541 gctcggcctc gacccggccg agatccgccg ccgcaacctc gtgcgcccgg agcagatgcc
     3601 gtaccgcccg gggctcactt acaaggacgg cgtgccggtg acgtacgacg ccggcgactt
     3661 tcccgcggcg ttcgagcgcg cgctggcgct gatagggtac gacgcctggc gcgcgcgcca
     3721 gcgtgagcgc ggccgggcca cgcagcgctt tggcatcggg ctcgcgtgct acgcccaggg
     3781 taccgggctc ggccccttcg aaggcgccac cgtccgcgtg gatccgagcg gcaaggtcta
     3841 cgtcgtaatc ggcgtcaccg gccagggcca gggccacgcg accacgctcg cccaggtctg
     3901 cgccgagtcg ctcggcgcac cggtcgacga cgtggagatc gtcgccggcg acacgtcggt
     3961 cttcccgttc ggcatgggca ccggtggcag ccgtgtgatg gccaacgccg gccccgcggt
     4021 ggcgcgcgcg gcccgctcgg tgcgcgcgcg ggccgcgcgc gtcgccgccg acgtgctcga
     4081 gtgcgcgccg gaagacgtgc gcatcaccga cgggcgcgcc cacgtcgccg gcgtgccgga
     4141 ccgcggcgtc ggcctcggcc ggctcgctca cgtggcgatc aggtcgaaga cgctccgcgg
     4201 cgccgtcggc gagcccgggc tcaacgcctg cgtctacttc cagccggaaa cggtcacgtg
     4261 ggcgttcggc acccacgccg ccgtggtcga ggtggacgcg gagacgtgcg cgacgaagct
     4321 ggtcgcctac tgcgtcgtcc acgacccggg tcgtgccatc aaccccatga tcgtggaggg
     4381 tcagctccag ggcggcgccg tgcaggggct cgccgccggt ctgatggagg agctcgtcta
     4441 cgacgcctcg ggtcagctcg tgaccggcag cctgatggac tacgcgatcc cgcgcgccga
     4501 cgacctgccg gcgatcgacg tcgcgctgac cgagcaccgc tcggcgatca acgagctggg
     4561 cgtcaagggc gtgggcgaga gcggcgccat ccccggcgcg gcggccatcg ccaacgccat
     4621 cgaggacgcc gtcgccgacc tcggcgtcgt catcggcgac gtgcccgtca cgcccgcgag
     4681 gctgttcgcg ttgctcgccg ccgcgacgca ccgctgatga ctggcaagag ctccagccac
     4741 gtggtggacc cgcggatgcc ctggtggctc tcgccggtcc ggggcgcgta gacgatggcc
     4801 caccatcgtt atggacaatt cgacatgtgg cggaacagcg ctgccacgga gctggcggag
     4861 gcgcgagagc tggccgcgct gctggagctg cgtgcccgcg atccggccca ggtgtccgcg
     4921 cgcgcctgct acctggagct cgtcggcgtg acgccaggca gtcgggtgct cgacgtgggc
     4981 tgtggcagcg gcgcggtcac tcgcgacgtg gcgcggcgcg tccgccctgc gggtcgcgtc
     5041 gtggggctcg atcctggccc gggcttcctg gccatcgcgg ccgagctcgt cgagcgcgaa
     5101 gggctggccg acgtcgtcga gcttcgcctc ggagacgcgc gagcactgcc cttcgcagac
     5161 gcggagttcg acgttgcgct cgcagtcacc accctggccc acgtacccga cggcgagcgg
     5221 gcgcttcccg agctcgtccg ggtggtccgg cccggcggcc gcgtcggcat cttcgacctc
     5281 gacggcgacg gcgccatcct ggcgcatccg gatcgaacgt tgacgcgacg gatcgttgcc
     5341 gcttcgtccg accacatcat ggtcgatggc tggctcgccc gccgacttcc ggcgttgctc
     5401 gcagcgagcg ggctggaaga catcggagtg cgtgccttca ctcccgtgga gcgcgacccc
     5461 gccggcttct acgcgaggct ggcggagcgc cgcgctgccg tcgccgtgca ggccggcgcc
     5521 atcaccgtgg acgagcagcg cgagtggctg gcggccctcc gcgccgagca ggccgccgga
     5581 cgcttcctcg gcggccagac ccatctcttc gtctggggta cccgccg
//
LOCUS       DATIAZ010000272         3559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1656555, whole genome shotgun sequence.
ACCESSION   DATIAZ010000272 DATIAZ010000000
VERSION     DATIAZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656555"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VLF19_09680"
     CDS             <1..733
                     /locus_tag="VLF19_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93564.1"
                     /translation="IAGLTAVGDRGAVAVSDLSLAVRAGEIVGVAGVSGNGQRELVEV
                     VAGQREPTSGSLRIAGGPYGRTRAEMRRHRVSVLIEEPLRNACVRAMSVAENLAFRSF
                     DEPPIAVGRLLVRRRAVRARGRELIARYRIRAAGPDARLDTLSGGNVQRAVLARELSR
                     EPGVLVAQNPCAGLDVAATAEIRARIMSARNAGAAVLLISEDLDELLELADRIVVMFE
                     GRLVHETPRETADVHVIGRHMASPA"
     gene            848..2344
                     /locus_tag="VLF19_09685"
     CDS             848..2344
                     /locus_tag="VLF19_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSE93565.1"
                     /translation="MTTTIVRGGRVLDIAGHRAEPADVLIEGDTIREIDRPGLAAPPG
                     AVTIDARDRLLMPGLVNAHTHGHGGLARGLLGDRVPLELLLTLGPAVNGNRSLEDKHL
                     SATLSAVELIRRGCTAFFDLYAEFPAPTVDGVHAAARAYADVGIRAVVAPMMADRTLY
                     QALPGLMEALSESARRQVLALSAAPWATSIATCRELLERWPFDRDQVRPALGPTIPLH
                     CTDEFLVACRDLAREHDVALQTHLAESKTQAVLGPVRYGGKTLTAHLGDLGLLGPRFS
                     AAHGVWLTRDDVRRLADAGASVAHNPLSNLRLGSGIAPVRMMLEAGLRVGVGTDSASS
                     SDAQNMFECTRLAAYLSRVRTPDYTRWVTVEEALTMATEGSAGVLGMDRIGRLAPGYK
                     ADVLFLDLGHPGYLPLNDVAVQMVMGESGAAIDSVMVGGRMVLERGRLLTVDEAKLRA
                     QAEAARARLAAANAEAIAAARALHPHVGAFCVAMASRPYDLDTREVGL"
     gene            complement(2351..2794)
                     /locus_tag="VLF19_09690"
     CDS             complement(2351..2794)
                     /locus_tag="VLF19_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93566.1"
                     /translation="MPDLAARLTNPPRSQRDAPPRLGTVEALLLGGLAAEALTWAEHA
                     LAVTRQRGERGHEALAVAALAGAHAALDDPARAERCYRDGPAESLEMRPVMARCHFGL
                     GRLQRALGPTVAGGDSLARAAQLFTDMGAPGVGERDPSRRMSCCR"
     gene            complement(2858..3355)
                     /locus_tag="VLF19_09695"
     CDS             complement(2858..3355)
                     /locus_tag="VLF19_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSE93567.1"
                     /translation="MSDLAAPFDEFQAVVRPEWIDHNGHMNMGYYLVVFDLATDAFFA
                     WVGLDAAYRRDHGATTFCLEAHVTYHREVRAGDPLRFTTLLLAHDAKRLHYIHAMYHA
                     AEEYLAATNELMSLHVSEATRRGAPMAPAVLARLALVQRAHNAVPRPPQVGRTIGLAS
                     RPATS"
BASE COUNT          474 a         1308 c         1309 g          468 t
ORIGIN      
        1 gatcgcgggg ctcacggccg tcggcgaccg tggcgccgtc gccgtcagcg acctgtcgct
       61 ggccgtgcgc gccggggaga tcgtgggcgt ggccggcgtg tcgggcaacg gccagcgcga
      121 gctggtcgag gtggtcgcgg gccagcgcga gccgacatcg ggcagtctcc ggatcgccgg
      181 cgggccctac gggcgcacgc gagccgagat gcggcgccac cgcgtctcgg tgctcatcga
      241 ggagccgctg cgcaacgcct gcgtgcgcgc gatgtccgtc gccgagaacc tcgccttccg
      301 cagcttcgac gagcctccga tcgcggtcgg gcgactcctg gtgcgccgcc gcgccgtgcg
      361 ggcgcggggc cgggagctga tcgcccgcta ccgcatccgc gccgccgggc ccgacgcccg
      421 cctcgacacg ctctcgggcg gcaacgtcca gcgcgccgtc ctcgcccgcg agctgtcgcg
      481 cgaacccggg gtcctcgtcg cccagaaccc atgcgccggg ctcgacgtgg cggcgacggc
      541 ggagatccgc gcgcgtatca tgagcgcgcg gaacgcgggc gcggccgtgc tcctgatcag
      601 cgaggacctg gacgagctgc tggagctggc cgaccgcatc gtcgtgatgt tcgaggggcg
      661 gctcgtccac gagactcccc gggagacggc ggacgtgcac gtcatcgggc gccacatggc
      721 cagtccggct tgatcgtacg ggggcggagg gccccacgag ccccggcgct ggggcacccc
      781 ggccggtgcg cctctcgacg gcgcggttcg ttcacagact gaacgaagag gggcgcggga
      841 ggaacacatg accaccacga tcgtccgcgg cggcagagtg ctggacatcg ccggccatcg
      901 cgcggagccg gccgacgtcc tgatcgaagg cgacacgatc cgcgagatcg accgcccggg
      961 cctcgcggcg ccgccgggcg ccgtgacgat cgacgcgcgc gaccggctgc tgatgccggg
     1021 cctcgtcaac gcgcacacgc acggccacgg cggtctggcg cgcgggctcc tcggcgaccg
     1081 ggtgccgctc gagctgctgc tgacgctcgg ccccgccgtc aatggcaacc ggtcgctgga
     1141 ggacaagcac ctctcggcga cgctctccgc ggtcgagctg atccgccgcg gctgcacggc
     1201 cttcttcgac ctgtacgcgg agttccccgc gccgaccgtg gacggcgtgc acgcggccgc
     1261 gcgcgcctat gccgacgtcg gcatccgcgc ggtcgtcgcc cccatgatgg ccgaccgtac
     1321 gctctaccag gcgctgccgg ggctcatgga ggcgctgtcc gagtccgcgc gccggcaggt
     1381 gctggcgctg agcgcggcgc cgtgggccac cagcatcgcc acctgccgcg agctgctcga
     1441 gcgctggcca ttcgaccgcg accaggtgcg cccggcgctc ggcccgacca tcccgctgca
     1501 ctgcaccgac gagttcctcg tcgcctgccg cgacctggcg cgcgagcacg acgtcgccct
     1561 gcaaacgcac ctggcggagt ccaagacgca ggcggtgctg ggtccggtgc ggtacggcgg
     1621 caagacgctc acggcgcacc tcggcgacct cggtctgctc ggcccgcgct tctcggccgc
     1681 gcacggcgtc tggctcaccc gtgacgacgt gcggcgcctg gccgacgccg gggcgagcgt
     1741 ggcccacaac ccgctgagca acctgcgcct gggctccggc atcgcgccgg tgcgcatgat
     1801 gctcgaggcc ggcctgcgcg tgggcgtcgg caccgactcg gcgtcgtcct ccgacgcgca
     1861 gaacatgttc gagtgcacgc gcctggccgc gtacctgtca cgcgtgcgta cgccggacta
     1921 cacgcgctgg gtgacggtgg aggaggcgtt gaccatggcc accgagggca gcgccggcgt
     1981 gctgggcatg gaccgcatcg gccggctggc accgggctac aaggccgacg tcctcttcct
     2041 cgatctcggc caccccggct atctgcccct caacgacgtg gccgtgcaga tggtcatggg
     2101 cgagagcggc gccgccatcg acagcgtcat ggtcggcggc cgcatggtgc tcgagcgggg
     2161 acgcctgctg acggtggacg aggccaagct gcgcgcgcag gccgaggccg cccgcgcccg
     2221 cctggccgcc gccaacgccg aggcgatcgc cgccgcgcgc gcgctccatc cccacgtggg
     2281 cgcgttctgc gtggcgatgg ccagccggcc ctacgacctc gacacgcgcg aggtcggact
     2341 ctgacggcta tcaccggcag caactcatcc ggcgcgacgg atctcgttca cccacgccgg
     2401 gggcccccat gtcagtgaac agttgcgccg cccgggcgag cgagtcgccg ccggcaacgg
     2461 tcggaccgag cgctcgctgc agtcgaccga ggccgaaatg gcagcgagcc atcacgggcc
     2521 gcatctcgag ggattccgcc ggcccgtcgc gataacaccg ctccgcccgc gccggatcgt
     2581 cgagggcggc gtgcgcgccg gcgagggccg cgaccgcgag ggcctcgtgc ccgcgctcgc
     2641 ctcgctggcg cgtcacggcg agtgcgtgct ccgcccaggt cagcgcttcg gcggcgaggc
     2701 cgccgagaag gagggcttct acggttccga gacgcggcgg cgcgtcacgc tgagaccttg
     2761 gaggattggt gaggcgcgcg gccagatcgg gcacaatgtc tcatcgggcg tggtgccggg
     2821 acggtcgcga cgcctggctc actaccgcgg caacggatca gctcgtcgcc gggcgcgagg
     2881 cgaggccgat ggtccgccct acctggggcg gacgcgggac cgcgttgtgc gctcgctgga
     2941 cgagcgccag acgcgcgagc accgccggag ccatcggcgc gccgcgccgc gtggcctcgc
     3001 tgacgtggag cgacatcagc tcgttggtcg ccgccagata ctcctcggcg gcgtggtaca
     3061 tcgcgtggat gtagtgcagc cgcttggcgt cgtgcgccag cagcagcgtc gtgaagcgca
     3121 gcggatcgcc ggcgcgcacc tcccggtggt aggtgacgtg cgcctccagg cagaaggttg
     3181 tcgcgccgtg gtcccgccgg tacgccgcgt cgaggcccac ccaggcgaag aacgcgtcgg
     3241 tggcgaggtc gaacacgacc aggtagtagc ccatgttcat gtgaccgttg tggtcgatcc
     3301 actcggggcg gacgacagcc tggaactcgt cgaacggcgc ggcgagatcg ctcatgtgcg
     3361 cattctagcg acatggaccc cgaacatggc ccgaagcccc tttagcacgc cccggcgaca
     3421 ccgtggcgcg cctcgtcacc cgacgtgccc ctggcctcct gaggcggaag ttcgccgacc
     3481 gtaggccgag gtggggtcgg gggcgcgggt ccgccgacct gttccccgcg cacggaaacc
     3541 ccgcgccacc tgaagatgg
//
LOCUS       DATIAZ010000273         7727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1524197, whole genome shotgun sequence.
ACCESSION   DATIAZ010000273 DATIAZ010000000
VERSION     DATIAZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524197"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="VLF19_09700"
     CDS             complement(<1..135)
                     /locus_tag="VLF19_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSE93568.1"
                     /translation="MRRAGAAKREVLPDPKFGSRLVTKFVNIMMIRGKKSTAERIMYD
                     A"
     gene            complement(139..522)
                     /gene="rpsL"
                     /locus_tag="VLF19_09705"
     CDS             complement(139..522)
                     /gene="rpsL"
                     /locus_tag="VLF19_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSE93569.1"
                     /translation="MPTINQLVRYGREAVRKKSKTPAMQACPQKRGVCIRVYTTTPKK
                     PNSALRKVARVRLTNGLEVTSYIPGVGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTL
                     DTAGVANRKQSRSKYGMKTPKGGGA"
     gene            complement(698..4888)
                     /gene="rpoC"
                     /locus_tag="VLF19_09710"
     CDS             complement(698..4888)
                     /gene="rpoC"
                     /locus_tag="VLF19_09710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSE93570.1"
                     /translation="MLTDRPFGSLIREEKPTKDLWGILDRHAKPITFDAIRIKLAAPQ
                     KIRDWSHGEVKKPETINYRTFKPERDGLFCARIFGPTKDYECACGKYKRMKFAGVVCD
                     KCGVEVTRARVRRERMGHIELASPVSHVWFFKGLPSRIGQLLDMSLRELEKILYFEEY
                     VVLDSRIAAVKKKDLVSVDKARKLGDEHGPDAIKFGMGAEAIRELLRDLDLDSLARDL
                     RAQMLGETSVQKRKKIVKRLKVIEAFLKSGNQPDWMILEVIPVIPPELRPLVPLDGGR
                     FATSDLNDLYRRVINRNNRLKRLMDLKAPEIIIRNEKRMLQEAVDALFDNGRRGRVIT
                     GPNNRPLKSLSDTLKGKQGRFRQNLLGKRVDYSGRSVIVVGPYLKLHQCGLPKKMALE
                     LFKPFILRKLEERGIASSIKAAKKYVEKERPEVWDILDDVIKEHPVLLNRAPTLHRPG
                     IQAFEPILVEGKAIEIHPLVCTAFNADFDGDQMAVHVPLSMEAQLEAQVLMLSANNIL
                     SPSNGAPIAVPTQDMVLGIYYLTKERPGTPEHPVRGEGRLFADSDEVRVAYDNEDIDL
                     QARIRLRWQGEVIETTVGRTVFNEVVPEPLRFLNQELKKKQVIDLVARCYNTLGNEET
                     VKFLDDLKDLGFRYATLSGLSIGIDDMHIPSSKGSLIARARQDVNEVEQQYQDGVITN
                     GERYNKVVDIWAHTTELIAEAMFHELEGGVAGGEFNPIYMMADSGARGSRQQIRQLAG
                     MRGLMAKPSGEIIETPITSNFREGLTVLEYFTSTHGARKGLADTALKTADSGYLTRRL
                     VDVAQDVIVSEYDCGTVKYIEATPLVEGGDIIQSLRDRVIGRAAAEDVRDPLSQEIIV
                     QANTEITEELAQKIEDAGIERVRIRSALICETKRGVCVMCYGRNLGSGRLAELGEAVG
                     IIAAQSIGEPGTQLTMRTFHIGGTASRVVEASKHEAKGVGVVKYHNLRTVQNRDGDLV
                     ATNRNGEIGIVDDRGRERERYPVVYGAKIKVKAEQKVKPRTVLVEWDPFTNPILTEFT
                     GRVEFQDISENMTVREEFDEVTGLARKVIIEDPEGRLQPGIIIRSVETEQAAGERHRY
                     PLPVGAHLTVADGAEVYAADILAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVIT
                     EIDGRVEMGGFVKGMRKIVIRADNGETKEYLIPRGKHISVHEGDRVKAGEALMDGSPN
                     PHDYLAVLGDRELQRYLVNEVQEVYRLQGVTINDKHIEIIVRQMLRRVRIEEVGDTDF
                     VVGELVDKFAFQEENERVLAQGKRPATAKPVLLGITKASLSTDSFISAASFQETTRVL
                     TEAAISGKTDDLRGLKENVIVGRLIPAGTGLGNYTRVEVLTPGGETVKPLDQVLTLEP
                     PPSPDGGEDAEGSVAAAG"
     gene            complement(4902..>7727)
                     /gene="rpoB"
                     /locus_tag="VLF19_09715"
     CDS             complement(4902..>7727)
                     /gene="rpoB"
                     /locus_tag="VLF19_09715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSE93571.1"
                     /translation="DSARALFRGMFMDARRYDLARVGRFMINKKLGIDAPLHIKTLRS
                     EDVVSVIRHLLMVKLGSKPTDDIDHLGNRRVRSVGELLENQFRVGLTRMERAVKERMS
                     ISDIAALMPHDLINAKPVSAVVKEFFGSSQLSQFMDQTNPLAELTHKRRLSALGPRGL
                     SRERAGFEVRDVHPTHYGRICPIETPEGPNIGLISSLSTYARTNEFGFIETPYRRVHA
                     GRVTDEIVFLTALEEEQFVIAQANAEIDRTGRFVQERVSARKGGEFRMVPPEELHFMD
                     VSPKQLVSVAASLIPFLENDDANRALMGSNMQRQAVPLLQPEAPLVGTGMEHVVARDS
                     GAVIVAKRPGVVEYVSADRIVVRAESRSKKTDPVQDLPLDIYNLTKYRRSNQNTCINQ
                     KPVVRKAQRVAAGDVVADGPGTDQGELALGRNVLVAFMPWGGYNFEDAILVSERLVKD
                     DRFTSIHIEEFEIQARDTKLGKEEITRDIPNVSEEALKDLDDSGIVRIGAKVKAGDIL
                     VGKITPKGETQLTPEEKLLRAIFGEKAGDVRDTSLTVPPGIEGTVVDVKVFSRRGVDK
                     DERAKSIEEEEIARLEKDYQDEIAMVEMERDQKLKNLLVGKTAVVELFDPTSKTQRVT
                     RKGDRIERPDLDNFSWNELKKVKVKEDEGLAQMIRRIEELAEEQIAIYDSMLEERIGR
                     LRRGDDLPPGVIKMVKVYVAVKRKLSVGDKMAGRHGNKGVVSKVLPEEDMPYLPDGTP
                     VEIVLNPLGVPSRMNVGQILETHLGWAARALGLWIASPVFDGATEEEIKDHLRQAGMP
                     QSGKTALHDGRTGKRFHQEVTVGQIYIMKLAHLVDDKMHARSIGPYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAHTLQEMLTVKSDDVEGRNRIYEAIVKGENFLEPGTPE
                     SFNVLVKELQSLALDVELVTKDGKKAS"
BASE COUNT         1092 a         2678 c         2485 g         1472 t
ORIGIN      
        1 cgcgtcgtac atgatccgct ccgcggtcga cttcttgccg cggatcatca tgatgttgac
       61 gaacttggtg acgagccggc tcccgaactt cgggtccggc aggacctcgc gcttcgccgc
      121 gcccgcccgt cgcatgcctt acgctccgcc gcccttgggc gtcttcatgc cgtacttcga
      181 ccggctctgc ttgcggttgg ccacgccggc ggtgtcgagc gtgccgcgga tgatgtgata
      241 gcgcacgccc ggcaggtcct tcacgcggcc accgcggatc atgacgatcg agtgctcctg
      301 aaggttgtga cccacgccgg ggatgtagga ggtgacctcc aggccgttcg tcagccgcac
      361 gcgcgcgacc ttccgcaggg cggagttcgg cttcttgggc gtcgtcgtgt agacgcggat
      421 gcagacgccc cgcttttggg gacatgcctg catcgcgggc gtcttcgact tcttccgcac
      481 cgcttcccgg ccgtagcgca cgagctgatt aatggtcggc atggctctcc atcgccgtca
      541 ccggcgacaa aaccctaact ttataaccga caacccctgt cctgtcaaca ctttacgccc
      601 atggtgcgct cgcctcacgg cccccgcccc ccgtcgggtc gccccggctc gagcgacgaa
      661 cttcattccc gcaccctcta cccggccccc tccggactta tcccgccgcc gccaccgaac
      721 cctcggcgtc ctctccgccg tccggcgagg gcggcggctc gagcgtgagc acctggtcga
      781 gcggcttcac cgtctcgccg cccggcgtca gcacttccac ccgggtgtag ttgccgagcc
      841 cggtgccggc cgggatgagc cggccgacga tgacgttctc cttcagaccc cgcaggtcgt
      901 ccgtcttgcc ggagatcgcg gcctccgtca gcacccgcgt ggtctcctgg aacgaggccg
      961 ccgagatgaa gctgtcggtc gacagcgagg ccttggtgat accgagcagc acgggcttgg
     1021 ccgtcgccgg ccgcttgccc tgcgcgagta ctcgctcgtt ctcctcctgg aaggcgaact
     1081 tgtcgaccag ctcgccgacc acgaagtccg tgtcgccgac ctcctcgatc cgcacccggc
     1141 gcagcatctg ccggacgatg atctcgatgt gcttgtcgtt gatcgtcacg ccctgcaggc
     1201 ggtagacctc ctgcacctcg ttcacgaggt agcgctgcaa ctcgcggtcg ccgaggacgg
     1261 ccagataatc gtgggggttc ggcgagccgt ccatcagggc ctctccggcc ttcacacgat
     1321 ctccctcgtg gacgctgatg tgcttccctc gggggatcag atactccttc gtctctccgt
     1381 tatccgctcg aatcacgatc ttccgcatgc ccttcacgaa gccgcccatc tcgacccggc
     1441 cgtcgatctc ggtgatgacc gcctgctcct tcggcttgcg cgcctcgaag agctcggcca
     1501 cccgcggtag accgccagtg atgtccttgg tcttggtcgt ctcacgcggg atcttggcca
     1561 ggatgtcggc ggcgtagacc tcggcgccgt cggccaccgt gaggtgggcc cccacgggca
     1621 gcgggtaacg gtgccgctcg cccgccgctt gctccgtctc cacgctccgg atgatgatgc
     1681 ccggctgcag gcgcccctcc ggatcctcga tgatgacctt ccgggcgaga cccgtgacct
     1741 cgtcgaactc ctcgcgcacc gtcatgttct cgctgatgtc ctggaactcg acccggcccg
     1801 tgaactcggt gaggatcggg ttggtgaacg gatcccactc gaccagcacc gtccggggct
     1861 tcaccttctg ctcggccttg accttgatct tcgcgccgta caccaccgga tagcgctcgc
     1921 gctcccggcc gcggtcgtcg acgatgccga tctcgccgtt ccggttggtg gcgacgaggt
     1981 cgccgtcgcg gttctgcacc gtgcggaggt tgtggtattt caccacgccg acgcccttgg
     2041 cctcgtgctt gctggcctcg acgacgcggc tggccgtgcc gccgatgtgg aacgtccgca
     2101 tcgtgagctg cgtgcccggc tcgccgatgg actgggcggc gatgatgccc accgcctcgc
     2161 ccagctcggc caggcggccg gagccgaggt tgcgtccata gcacatcacg cacacgcccc
     2221 gcttcgtctc gcagatgagg gccgagcgga tccggacccg ctcgataccc gcgtcctcga
     2281 tcttctgggc gagctcctcg gtgatctccg tgttcgcctg gacgatgatt tcctgcgaca
     2341 gcggatcgcg gacgtcctcc gcggccgccc ggccgatcac gcggtcacgc agggactgga
     2401 tgatgtcacc accttcgacc agcggcgtgg cctcgatgta cttgacggtg ccacagtcgt
     2461 actcgctgac gatgacgtcc tgggccacgt cgacgaggcg gcgcgtcagg tagccggagt
     2521 cggcggtctt gagcgcggtg tcggcgagac ccttgcgggc gccgtgcgtc gaggtgaaat
     2581 actccagcac cgtgaggccc tcacggaagt tcgacgtgat cggcgtctcg atgatctcgc
     2641 ccgacggctt ggccatgagg ccgcgcatgc cggcgagctg ccggatctgc tgccgcgaac
     2701 cccgggcacc ggagtccgcc atcatgtaga tggggttgaa ctcgccgccg gcgacgccgc
     2761 cctccagctc gtggaacatc gcctccgcga tcagctcggt ggtgtgcgcc cagatgtcga
     2821 ccaccttgtt gtaccgctcg ccgttggtga tgacgccgtc ctggtactgc tgctcgacct
     2881 cgttgacgtc ctgccgggcc cgcgcgatca gcgacccctt cgacgaaggg atgtgcatgt
     2941 cgtcgatgcc gatcgacagg cccgagagcg tcgcgtaacg gaagccgagg tccttcaggt
     3001 cgtcgaggaa cttcacggtc tcctcgttgc cgagcgtgtt gtagcagcgg gcgacgagat
     3061 cgatgacctg cttcttcttc agctcctggt tcaggaatcg cagcggctcc ggcaccacct
     3121 cgttgaagac ggtccgaccg accgtcgtct cgatgacctc cccctgccag cgcagcctga
     3181 tgcgggcctg gaggtcgatg tcctcgttgt cgtaggccac ccgcacctcg tcggagtcgg
     3241 cgaacagccg gccctcgccc cggacggggt gctcgggcgt gccggggcgc tccttggtca
     3301 ggtagtagat gccgagcacc atgtcctgcg tgggcacggc gatcggcgcc ccgttcgagg
     3361 gtgacaggat gttgttggcc gacagcatca gcacctgggc ctcgagctgg gcttccatcg
     3421 acagcggcac gtggaccgcc atctggtcgc cgtcgaagtc cgcgttgaac gccgtgcaca
     3481 ccagcgggtg gatctcgatg gccttgccct cgacgaggat gggctcgaag gcctggatgc
     3541 ccgggcgatg gagcgtcggc gcccggttga gcagcaccgg gtgctccttg atgacgtcgt
     3601 cgaggatgtc ccacacctcg ggccgctcct tctccacgta cttcttggcg gccttgatcg
     3661 acgacgcgat cccgcgctcc tcgagcttgc ggaggatgaa cggcttgaac agctcgagcg
     3721 ccatcttctt gggcagtccg cactggtgca gcttcagata cggtcccacg acgatgaccg
     3781 atcggcccga gtaatccacg cgcttgccga gcaggttctg gcggaagcga ccctgcttgc
     3841 ccttgagcgt gtcggagagg gacttcagcg gccggttgtt ggggccggtg atgacccggc
     3901 cgcgccgccc gttgtcgaac agcgcgtcca cggcctcctg cagcatccgc ttctcgttgc
     3961 ggatgatgat ctcgggagcc ttcaggtcca tgaggcgctt caaccggttg ttccggttga
     4021 tcacgcgccg gtagaggtcg ttcaggtccg aagtcgcgaa ccggccgccg tcgagcggca
     4081 ccagcgggcg tagctcgggc gggatgacgg ggatgacctc caggatcatc cagtccggct
     4141 ggttgccgga cttgaggaag gcctcgatca ccttcagccg cttgacgatc ttcttgcgct
     4201 tctgcaccga cgtctcgccc agcatctgcg cgcgcaggtc gcgggccagc gagtcgaggt
     4261 cgaggtcgcg cagcagctcg cggatggcct ccgcgcccat gccgaacttg atggcgtcgg
     4321 gaccgtgctc gtcgccgagc ttgcgggcct tgtccaccga cacgaggtcc ttcttcttga
     4381 ccgcggcgat ccgcgagtcg aggacgacgt actcctcgaa gtacaggatc ttctccagct
     4441 cacgcagtga catgtcgagg agctggccga tgcggctcgg cagccccttg aagaaccaca
     4501 cgtggctgac cggcgaggcc agctcgatgt ggcccatgcg ctcgcggcgc acgcgggcgc
     4561 gggtcacctc gacgccgcac ttgtcgcaca cgacgccggc gaacttcatg cgcttgtact
     4621 tgccgcacgc gcactcgtag tccttcgtcg gcccgaagat gcgggcgcag aacaacccgt
     4681 cccgctcggg cttgaacgtc cggtagttga tggtctcggg cttcttcacc tcgccgtgcg
     4741 accagtcgcg gatcttctgc ggcgcggcga gcttgatccg gatcgcgtcg aacgtgatgg
     4801 gcttcgcatg ccgatcgagg atgccccaga gatccttggt gggcttctcc tccctgatca
     4861 ggctgccgaa aggccgatcc gtcagcattc gcactctcct ctcaggacgc tttcttcccg
     4921 tctttggtca ccagttcgac gtcgagggcg aggctctgga gctccttgac cagcacgttg
     4981 aaggactccg gcgtgccggg ctcgaggaag ttctcaccct tgacgatggc ctcgtagatg
     5041 cggttccgtc cctccacgtc gtcggacttg acggtgagca tctcctgaag cgtgtgcgcg
     5101 gcgccgtagg cctcgagcgc ccacacctcc atctcgccga accgttgccc tccgaactgc
     5161 gctttgccgc cgagcggctg ctgggtgacg agcgagtacg gaccgatcga gcgcgcgtgc
     5221 atcttgtcgt cgaccaggtg cgcgagcttc atgatgtaga tctggccgac cgtgacttcc
     5281 tgatggaacc gcttgccggt gcggccgtcg tggagcgcgg tcttgccgga ctgcggcatg
     5341 ccggcctgcc ggaggtggtc cttgatctcc tcctcggtgg cgccgtcgaa gaccgggctg
     5401 gcgatccaca ggccgagcgc ccgcgcggcc caccccaggt gcgtctcgag aatctgcccc
     5461 acgttcatgc gcgagggcac gccgaggggg ttcagcacga tctccacggg cgtaccgtcc
     5521 ggcaggtacg gcatgtcctc ctcgggcagc accttggaga ccacgccctt gttgccgtgg
     5581 cgccccgcca tcttgtcgcc gaccgagagc ttccgcttga cggcgacgta gaccttcacc
     5641 atcttaatca cacccggcgg caggtcgtcg ccccggcgca ggcggccgat gcgctcctcc
     5701 agcatcgagt cgtagatggc gatctgctcc tccgccagct cctcgatgcg gcggatcatc
     5761 tgcgcgaggc cctcgtcctc cttcaccttg accttcttca gctcgttcca ggagaagttg
     5821 tcgaggtcgg gccgctcgat gcggtcgccc ttcctggtga cccgctgcgt cttgctggtg
     5881 ggatcgaaca gctcgaccac ggccgtcttc ccgaccagga ggttcttgag cttttggtcg
     5941 cgctccatct cgaccatggc gatctcgtcc tggtaatcct tctccaggcg cgcgatctcc
     6001 tcctcctcga tcgacttcgc ccgctcgtcc ttgtcgaccc cgcggcgaga gaagaccttc
     6061 acgtccacca ccgtgccctc gatgccgggg ggcaccgtga gcgaggtgtc gcgcacgtcg
     6121 ccggccttct cgccgaagat cgcgcgcagc agcttctctt ccggcgtcag ctgcgtctcg
     6181 cccttgggcg tgatcttgcc gaccaggatg tcgccggcct tgaccttggc gccgatgcgg
     6241 acgattcccg agtcgtccag gtccttgagg gcttcctccg acacgttcgg gatgtcgcgg
     6301 gtgatctcct ccttgccgag cttcgtgtcg cgcgcctgga tctcgaactc ctcgatgtgg
     6361 atcgaggtga agcggtcgtc cttcacgagc cgctcggaga ccaggatcgc gtcctcgaag
     6421 ttgtagccgc cccacggcat gaaggcgacc agcacgttgc ggcccagggc cagctcgccc
     6481 tggtcggtgc cggggccgtc ggccacgacg tcgcccgccg ccacccgctg ggccttgcgc
     6541 accaccggct tctggttgat gcaggtgttc tggttggagc gccgatactt ggtcaggttg
     6601 tagatgtcga ggggcaggtc ctgcacaggg tcggtcttct tcgaccggct ctccgcccgc
     6661 acgacgatgc ggtccgccga cacgtactcc accacgccgg gccgcttggc cacgatcacg
     6721 gcgcccgagt cgcgggcgac gacgtgctcc atgcccgtgc cgaccagcgg cgcctccggc
     6781 tggagcagcg gcaccgcctg ccgctgcatg ttcgagccca tcagcgcgcg gttggcgtcg
     6841 tcgttctcga ggaaggggat cagcgacgcc gctaccgaga cgagctgctt ggggctgacg
     6901 tccatgaagt gcagctcctc cgggggcacc atgcggaact ccccaccctt gcgcgcggag
     6961 acccgctcct ggacgaaccg gccggtgcgg tcgatctcgg cgttggcctg ggcgatgacg
     7021 aactgctcct cctcgagcgc ggtcaggaac acgatctcgt cggtgacccg gcccgcgtgc
     7081 actctccggt agggcgtctc gatgaagccg aactcgttgg tgcgagcgta ggtggacagc
     7141 gacgagatca ggccgatgtt cggtccttcg ggcgtctcga tcgggcagat gcggccgtag
     7201 tgggtcggat ggacgtcgcg cacctcgaac ccggcacgct cgcggctgag gccgcgagga
     7261 ccgagggccg acagccgccg cttgtgggtc agctccgcca gcgggttcgt ctggtccatg
     7321 aactgggaca gctgcgagga gccgaagaac tccttcacca ccgccgagac cggcttggcg
     7381 ttgatgaggt cgtggggcat gagcgccgcg atgtccgaga tggacatgcg ctccttcacc
     7441 gcgcgctcca tgcgggtgag gcccacgcgg aactggttct cgagcagctc gccgaccgac
     7501 cgcacccgcc ggttgccgag gtggtcgatg tcgtcggtcg gcttgctgcc gagcttcacc
     7561 atcagcaggt gccggatcac ggacaccacg tcctcgctgc gcagcgtctt gatgtgcagt
     7621 ggagcgtcga tgccgagctt cttgttgatc atgaaccggc cgacccgcgc cagatcgtag
     7681 cgccgcgcgt ccatgaacat gccgcggaac agcgcgcggg cagagtc
//
LOCUS       DATIAZ010000274         8239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_341816, whole genome shotgun sequence.
ACCESSION   DATIAZ010000274 DATIAZ010000000
VERSION     DATIAZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341816"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /gene="hisB"
                     /locus_tag="VLF19_09720"
     CDS             <1..497
                     /gene="hisB"
                     /locus_tag="VLF19_09720"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSE93572.1"
                     /translation="PIPFFSHMLEAWAKHGLMDLGVEAAGDVEVDIHHTVEDVGIVLG
                     QALRQALGDRRGIVRFGTAFVPMDEALIASSVDISGRPFLVFNVPVARTRVSNFDLDM
                     LQEFFRALAFNAEITLHVTMHYGHNLHHISEAVFKAVGRALAAATRIDPRIAGMLPST
                     KGTL"
     gene            580..1197
                     /gene="hisH"
                     /locus_tag="VLF19_09725"
     CDS             580..1197
                     /gene="hisH"
                     /locus_tag="VLF19_09725"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSE93573.1"
                     /translation="MIAVVDYGRGNLGSVEKAFERVGTPAVVTQDPRAVDEADAVVLP
                     GDGAFHDAMSNLAGLGLMDAMRRALDGRRPFLGICLGYQLLFTESEEFGSGRGLDVVS
                     GVVRRFPAGRKVPHMGWNRVSHEGRLRLLEGVPDGAHFYFVHSYYPVVADGAGARVAW
                     CEYGVRFAAALERGRLFATQFHPEKSQRWGIRLLENFAAIVKEAA"
     gene            1194..2012
                     /gene="hisA"
                     /locus_tag="VLF19_09730"
     CDS             1194..2012
                     /gene="hisA"
                     /locus_tag="VLF19_09730"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HSE93574.1"
                     /translation="MIVVPAVDLKAGRCVRLRQGRADQETVFSDDPVAMARRWAEAGA
                     ARLHVVDLDGAFMGAPRQTAVIAKIAAAVAPVPVEAGGGLRDLGAVEAMLETGVRWAV
                     IGTRAALDPGFLGDVGRRFPDRIVVAVDGKGSRVAVKGWTETAEVGVVEVAQRALDAG
                     AAAVLYTDVSRDGTGRGPNVEATAALARVVDVPVLASGGVGTVDHLVALAAVPGVAGT
                     IVGRALYTGAIDLQQAIDVLRSSGGSASIDGEGLGGGRRGPFRGGMGGDPPLRA"
     gene            2015..2779
                     /gene="hisF"
                     /locus_tag="VLF19_09735"
     CDS             2015..2779
                     /gene="hisF"
                     /locus_tag="VLF19_09735"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSE93575.1"
                     /translation="MLAKRVIPCLDVKDGRVVKGVRFVDLRDAGDPVEAALAYDAQGA
                     DELVFLDITASHEDRKIMLDVVARTAEGIYMPLTVGGGIREIDDVRALLRAGADKVSL
                     NTAALERPALIREAAERFGSQCIVVAIDARRDSAGRWGVYTHGGRRRAGRDAVVWARE
                     ATALGAGEILLTSMDRDGTKDGYDLELTRAVSQSVEVPVIASGGAGSLEHLFEGLVEG
                     QADAALAASIFHFGIHTIGEAKRYLRQRGVEVRLEP"
     gene            2776..3408
                     /gene="hisIE"
                     /locus_tag="VLF19_09740"
     CDS             2776..3408
                     /gene="hisIE"
                     /locus_tag="VLF19_09740"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HSE93576.1"
                     /translation="MTLDELKLDAEGLIPAVVQEAETGEVLMVAWMDRPAVDRTLATG
                     LTHFWSRSRRQPWQKGETSGHVQHVQGVYADCDGDVLLVQVHQEGVACHTGHRTCFFT
                     ALQGDGAAGGMLLRLERIVAARRTGAPAGSYVGTLLAKGEAAISRKIGEEATEVITAA
                     LGGEGDRRVVEEVADLWFHTIVLLGARGIPLSDVLEELARRHRARSTPGA"
     gene            3405..3920
                     /locus_tag="VLF19_09745"
     CDS             3405..3920
                     /locus_tag="VLF19_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93577.1"
                     /translation="MSAREARVAVLGLVAALALATGCAGRRVEHGVFHSPKGYRAALP
                     GDAWSVVNGSRADLELRHASGRAGMTVNATCDPAVARRPAAQLTRALFAGLRDRSDVE
                     HGDAVVGGRTAARTVVEGRAGMDGDRLRVEALTVVDGGCVYDLLYAAPTESFDAWHDD
                     FMRFAASFAKE"
     gene            3923..4684
                     /locus_tag="VLF19_09750"
     CDS             3923..4684
                     /locus_tag="VLF19_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93578.1"
                     /translation="METYLRRTATWYGGVGLLGGSVLRNLALPPAYLAHVIREIEIMG
                     VRSLGVALTAAVFTGMVFALQSAVNMARFGAENYVGPLTALAILRELGPVLTAILVGG
                     KVASGITAELGSMKVTEQIDALRTLGVNYVKRLIVPRILAALIVFPLLTVLADAVGVL
                     GGMVVMFFERGADMYAYWNTTTYWVVPRDFLMGVGKSVFFGIIVTLIGCYNGLTTQGG
                     TEGLGRATTATVVHVTMGVIVSDFFLTKLFLILFW"
     gene            4681..5475
                     /locus_tag="VLF19_09755"
     CDS             4681..5475
                     /locus_tag="VLF19_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93579.1"
                     /translation="MSDHVASGRGAARTAGDLLIDVVDVWKAFDTTPVLRGVSLTLAR
                     GETLVVMGGSGSGKTVLLRLIAGLLRPDRGHIRLFGTNIERLSEEELLPVRRRMGYVF
                     QGSALFDSLTVLENVAYPLREHTRLDDAEIRDRVAKFLGLVGLGEEVLPLVPSELSGG
                     MRKRVGIARALVIEPEVLLFDEPTAGLDPTNSRLVGDLISQVHTGVCDTAIVVTHDLE
                     LTKTVADRVAILIDGRFAAVGPLAEVLGSDVPEVHAFLEGRSGVRT"
     gene            5472..6671
                     /locus_tag="VLF19_09760"
     CDS             5472..6671
                     /locus_tag="VLF19_09760"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSE93580.1"
                     /translation="MNEERSERGLKLRVGVFVLVALAAFLGMIYALGARARLFEARYT
                     IHADFTEVGGLAEGATVRLAGVQIGRVSGVHLPGEPGGKVRVDLTIARRYADRIRKDS
                     AARIETQGLLGDKIVEITVGTPRTTVVQPGETIAARDPTDITQVINESAVTVKNVAAL
                     ADRLRELAEGVAKAKIIDDVSAATASARRAADQLARLTTEVEKGRGWAHALIYEEPVA
                     LKRLGELVASTGRILERAERGEGALGVLTSRQSTESARKFLAAMDRVARLTEGPRAED
                     GLLPALLFDPKYRAVLDDLQAVTRNLREVSDRVVGGRGTLGSLVKDESEDGKLRSTME
                     DLQVAVANLKEITEKINEGEGTLGALIADPTIYERLAAILDGTQRSVILRSLIRNLGK
                     DQRDGTE"
     gene            6676..8043
                     /gene="radA"
                     /locus_tag="VLF19_09765"
     CDS             6676..8043
                     /gene="radA"
                     /locus_tag="VLF19_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSE93581.1"
                     /translation="MSARERSVWRCQQCGFASPKPGTCPDCRRATGELVALIEERPVA
                     VAGARRSAMPPSRPRPLKDVSLDRTERLRTGIGELDRVLGGGVVPGSLALIGGDPGVG
                     KSTVLLQAARALAEAAPPVLYVSGEESAAQVKLRADRLGVTTDGLLLWSENDLSAVQA
                     ELDTVKPRALVVDSIQTVFLPELESAPGSVAQVRECGARLMTLAKGRGLATFIVGHVT
                     KDGSLAGPRVLEHLVDTVLYFEGEQHHAYRVLRAVKNRFGSTNEIGVFQMGERGLMEV
                     PNPSGFFLAERPKDAPGSVIVAGLEGTRPLLLELQALVTPASFGTPRRTVIGADYNRT
                     CLLLAVLEKRAGVPLGTQDVFVNVAGGARIVEPAADLGVVVAAASSYLDRPVRGDVVV
                     VGEVGLTGEVRAITGLEARLREAAALGFREAVVPQSNLAEQPRLPLEARGVTTVHAAL
                     DLLVG"
     gene            8090..>8239
                     /locus_tag="VLF19_09770"
     CDS             8090..>8239
                     /locus_tag="VLF19_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSE93582.1"
                     /translation="MPGPPRAARAVRDVAVLVPAAGAGARMGGRTPKQFLALGGAPIL
                     AVTVRR"
BASE COUNT         1104 a         2804 c         3146 g         1185 t
ORIGIN      
        1 ctccgatccc gttcttcagc cacatgctgg aggcgtgggc gaagcacggc ctcatggacc
       61 tcggcgtcga ggccgccggg gacgtcgagg tggacatcca ccacaccgtc gaggacgtcg
      121 gcatcgtgct cgggcaggcc ctgcgccagg cgctcggcga ccggcggggc atcgtgcgct
      181 tcggaaccgc cttcgtgccg atggacgagg cgctcatcgc ctcgtcggtg gacatctcgg
      241 gccggccgtt cctggtgttc aacgtgcccg tggcccgcac gcgcgtgtcg aacttcgacc
      301 tcgacatgct ccaggagttc ttccgggccc tcgccttcaa cgccgagatc acgctgcacg
      361 tgaccatgca ctacggtcac aacctgcacc acatcagcga ggcggtgttc aaggcggtgg
      421 gtcgcgcgct ggctgcggcc acgcgcatcg atccgcgtat cgcgggaatg ctgccgtcca
      481 cgaaggggac gctgtgaacg acgtggccgt gcgcgccgcc ggccggagca agagccgcag
      541 ccggaggcga ggcgaccctg tcaggatcga ggccagccgg tgatcgcggt cgtcgactac
      601 ggccgcggga acctcggctc ggtggagaag gccttcgagc gcgtcggcac gccggccgtg
      661 gtcacgcagg acccccgcgc ggtggacgag gccgacgccg tcgtgctgcc cggcgacggc
      721 gccttccacg acgcgatgtc gaatctggcc ggcctgggcc tgatggacgc catgcgccgc
      781 gcgctcgatg gccggcgccc cttcctgggc atctgcctgg gctatcagct gctcttcacg
      841 gagagcgagg agttcgggtc gggccggggg ctcgacgtcg tctccggcgt ggtccggcgc
      901 ttccccgcgg gccgcaaggt gccgcacatg ggctggaacc gcgtgagcca cgaagggagg
      961 ctgcggctcc tcgagggcgt accggacggc gcgcacttct acttcgtgca ctcgtactat
     1021 ccggtcgtcg cggacggcgc cggcgcgcgg gtggcctggt gcgagtacgg cgtcagattc
     1081 gcggcggcgc tcgagcgcgg gcgcctcttc gccacgcagt tccatccgga gaagagccag
     1141 cgctggggga tccggctgct cgagaacttc gcggcgatcg tcaaggaggc ggcgtgatcg
     1201 tcgtccccgc cgtcgacctg aaggccgggc gctgcgtgcg tctgcggcag ggccgcgccg
     1261 accaggagac ggtcttctcc gacgacccgg tcgccatggc gcgccgctgg gccgaggccg
     1321 gagcagcccg cctgcacgtg gtggacctcg acggcgcctt catgggcgcc ccgcggcaga
     1381 ccgcggtcat cgcgaagatc gcggcggcgg tggcgccggt gccggtggag gcgggcggcg
     1441 ggctgcgcga cctcggggcc gtcgaggcga tgctcgagac cggcgttcgc tgggccgtga
     1501 tcggcacccg ggcggcgctc gatcccgggt tcctcggcga tgtcggccgc cgcttcccgg
     1561 atcgtatcgt cgtcgccgtg gacggcaagg ggtcgcgggt cgccgtcaag ggatggacgg
     1621 agaccgccga ggtgggtgtc gtagaggtgg cgcagcgggc gctcgacgcg ggcgcggcgg
     1681 cggtcctcta caccgacgtg agccgcgacg gcaccgggcg cggcccgaac gtcgaggcca
     1741 ccgcggccct cgcccgcgtc gtggacgttc cggtgctcgc ttccggcggc gtcggcacgg
     1801 tcgatcacct ggtcgccctc gccgccgtcc cgggcgtggc cggaacgatc gtgggccgcg
     1861 ccctctatac cggcgccatc gacctgcagc aggcgatcga cgtgcttcgt tcgagcggcg
     1921 gctccgcctc catcgatggg gaaggtctcg gagggggccg gcgaggcccc ttccgaggag
     1981 ggatgggggg ggaccccccc ctgcgagctt aggcatgctg gcgaagcgcg tcatcccctg
     2041 cctcgacgtg aaggacggcc gcgtcgtgaa gggcgtgcgg ttcgtcgatc tgcgcgacgc
     2101 cggggacccc gtggaggccg cgctcgccta cgacgcccag ggcgccgacg agctggtctt
     2161 cctcgatatc acggcgtcgc acgaggaccg gaagatcatg ctcgacgtcg tcgcgcggac
     2221 cgcggagggc atctacatgc cgctcaccgt cggcggcggc atcagggaga tcgacgacgt
     2281 ccgggcgctc ctccgcgcgg gcgccgacaa ggtgtcactc aacacagcgg cgctcgagcg
     2341 gccggcactg atccgcgagg ccgccgagcg cttcggcagc caatgcatcg tggtggctat
     2401 cgatgcgcga cgcgacagcg ctggccgctg gggcgtgtac acgcatggcg gtcggcgccg
     2461 cgccggacgc gacgcggtcg tgtgggcgcg ggaggcgacg gccctcggcg cgggcgagat
     2521 cctcctgacg agcatggacc gagacggcac caaggacggc tacgacctcg agctgacgcg
     2581 tgcggtctcg cagtccgtgg aggttccagt gatcgcgtcc ggcggcgcgg gctcgctcga
     2641 acacctcttc gaagggctcg tggaggggca ggccgacgcc gcgctggcgg cgtcgatctt
     2701 tcacttcggc atccacacga tcggggaggc caagcgctac ctgcgacagc gcggcgtcga
     2761 ggtgaggctc gagccgtgac gctcgacgag ctgaagctcg acgccgaggg cctgatcccc
     2821 gccgtcgtcc aggaggcgga gaccggcgag gtgctgatgg tcgcgtggat ggaccgcccg
     2881 gcggtggaca ggacgctcgc caccggactc acgcacttct ggtcgcgctc gcggcggcag
     2941 ccgtggcaga agggcgagac ctccggtcac gtccagcacg tgcagggcgt ctacgccgac
     3001 tgcgacggcg acgtgctgct ggtccaggtg caccaggagg gcgtggcctg ccacaccggc
     3061 caccgcacgt gcttcttcac ggccctgcag ggcgacggcg cggcgggggg catgctcctg
     3121 cgcctcgagc ggatcgtcgc cgcgcggcgg accggcgcgc ccgcgggatc gtacgtgggg
     3181 accctcctgg ccaaaggcga ggcggcgatc tcgcgcaaga tcggcgagga ggcgaccgag
     3241 gtgatcaccg ccgccctcgg tggcgagggc gaccgccgcg tcgtcgaaga agtggccgac
     3301 ttgtggttcc acacgatcgt cttgctggga gcgcgcggca tccctctgag cgacgtgctc
     3361 gaggagctcg cgcgacgaca ccgggcccga tccaccccgg gcgcgtgagc gcacgggagg
     3421 cacgcgtcgc ggtgctgggc ctcgtggcgg ctctggcgct ggccaccggc tgcgccgggc
     3481 ggcgcgtcga gcacggcgtc ttccactcgc ccaaggggta tcgcgcggca ctcccgggcg
     3541 acgcctggtc ggtggtgaac ggaagccgcg cggacctcga gctccgccac gcgagcgggc
     3601 gcgcgggcat gaccgtgaac gcgacctgcg atcccgcggt cgcgcgccgt cccgccgccc
     3661 agctgacgcg cgcgctcttc gccgggctgc gcgaccggtc cgacgtggag cacggcgacg
     3721 ccgtggtcgg cgggcggacg gccgcgcgca ccgtcgtcga ggggcgcgcc gggatggacg
     3781 gcgatcgcct ccgcgtcgag gcgctgaccg tcgtggacgg cggctgtgtc tacgacctgc
     3841 tctacgcggc gcccacggag tcgttcgacg cctggcacga cgacttcatg cggttcgccg
     3901 cgagcttcgc gaaggagtga cgatggagac atatctgaga cgtacggcga cgtggtacgg
     3961 cggcgtgggt ctgctgggcg gcagcgtgct gcgcaacctc gcgctcccgc cggcctatct
     4021 cgcccacgtg atccgcgaga tcgagatcat gggcgtgcgg tcgctgggcg tcgcgctgac
     4081 agccgccgtc ttcaccggca tggtgttcgc actccagagc gccgtgaaca tggcccggtt
     4141 cggcgccgag aactacgtgg ggccgctgac ggcgctggca atcctgaggg agctggggcc
     4201 cgtgctcacg gcgatcctgg tcggcggcaa ggtcgcctcg ggcatcacgg cggagctggg
     4261 ctcgatgaag gtcaccgagc agatcgatgc tctccgcacg ctcggggtca actacgtcaa
     4321 gcgcctcatc gtgccgcgga tccttgccgc gctcatcgtg ttcccgctgc tgaccgtcct
     4381 cgccgacgcc gtcggcgtgc tggggggcat ggtcgtgatg ttcttcgagc gtggggcgga
     4441 catgtacgcg tactggaaca cgacgacgta ctgggtggtg ccgcgggact tcctgatggg
     4501 ggtcggcaag agcgtcttct tcggcatcat cgtcacgctc atcggctgct acaacggcct
     4561 caccacccag ggcggcaccg aggggctcgg tcgggcgacg acggcgacgg tcgtgcacgt
     4621 caccatgggc gtcatcgtct cggacttctt cctgacgaag ctcttcctca tcctcttctg
     4681 gtgagcgacc acgtggcgag cgggcggggc gcggcgcgga cggcgggcga tctcctgatc
     4741 gacgtcgtcg acgtgtggaa ggcgttcgac acgacaccgg tgctgcgcgg cgtgtcgctg
     4801 acgctggcca ggggcgagac gctggtcgtc atgggcggca gcggatccgg caagaccgtc
     4861 ttgctgcggc tgatcgcggg gctgctgcgg cctgaccgcg gccacatccg gctcttcggg
     4921 acgaacatcg agcggctgtc ggaagaggag ctgctgcccg tccgccggcg catgggctac
     4981 gtcttccagg ggtccgcgct cttcgactcg ctcacggtgc tcgagaacgt ggcctacccg
     5041 ctccgtgagc acacgcggct cgacgacgcg gagatccggg accgcgtggc caagttcctc
     5101 gggctcgtgg ggctgggcga ggaggtcctg ccgctggtgc cctcggagct gtccggcggc
     5161 atgcgcaagc gcgtgggcat cgcgcgggcg ctggtgatcg agccggaggt gctgctgttc
     5221 gacgagccga cggcagggct cgaccccacg aactcccggc tcgtcggcga cctgatctcg
     5281 caggtccaca cgggcgtgtg cgacactgcg atcgtcgtga ctcacgacct cgagctgacc
     5341 aagacggtgg cggaccgcgt ggccatcctc atcgacggcc ggttcgccgc ggtaggcccg
     5401 ctggcggaag tgctgggctc ggatgtcccg gaagtccacg cgttcctgga gggccggagc
     5461 ggggtgcgga cgtgaacgag gagcggagcg agcggggcct gaagctgcgg gtcggcgtgt
     5521 tcgtgctggt ggcgctggcg gccttcctcg gcatgatcta cgcgctcggc gcgcgggcga
     5581 ggctcttcga ggcccgctac acgatccacg ccgacttcac cgaggtcggc ggcctcgccg
     5641 agggcgccac ggtccgcctg gccggcgtgc agatcggacg cgtgagcggc gtgcacctgc
     5701 cgggggagcc gggcggcaag gtgcgcgtgg acctcaccat cgcccggcgg tacgccgacc
     5761 gcatccggaa ggactccgcg gctcgcatcg agacccaggg cctgctcggc gacaagatcg
     5821 tcgagatcac cgtggggacg ccccgcacga ccgtggtgca gccgggggag accatcgcgg
     5881 cccgcgaccc caccgacatc acgcaggtca tcaacgagag cgcggtgacg gtgaagaacg
     5941 tggccgccct cgcggacagg ctgcgcgagc tggccgaggg cgtggccaag gcgaagatca
     6001 tcgacgacgt gtccgcggcc accgcctccg cccggcgggc cgccgaccag ctcgcccggc
     6061 tgacgacgga ggtggagaag gggcgcggct gggcccacgc gctgatctac gaggagcccg
     6121 tcgcgctgaa gcggctcggc gagctggtcg cctcgacggg gcggatcctc gagcgggcgg
     6181 agcgcggcga gggcgcgctc ggcgtgctga cgtcccggca gtcgacggaa tccgccagaa
     6241 agttcctggc cgcgatggac cgggtggccc gcctgactga ggggccgcgc gcggaggacg
     6301 ggctcctgcc ggcgctgctg ttcgatccga agtaccgggc ggtcctggac gacttgcagg
     6361 ccgtgacccg taaccttcgt gaggtgtcgg accgggtcgt cggcgggcgg ggcacgctgg
     6421 gcagcctcgt caaggacgag agcgaggacg gcaagctccg ctccacgatg gaggatctcc
     6481 aggtcgccgt ggcgaatctc aaggagatca ccgagaagat caacgagggg gaggggacgc
     6541 tgggcgccct gatcgcggat cccacgatct acgagcggct ggcggcgatc ctggacggca
     6601 cgcagcgcag cgtgatcctg cgctccctca tccgcaacct cggcaaggac cagcgcgacg
     6661 ggacggaatg accgcgtgag cgcccgcgag cggagcgtgt ggcggtgcca gcagtgcggc
     6721 ttcgcgagcc cgaagcccgg cacctgcccc gactgcaggc gggcgaccgg cgagctggtc
     6781 gcgctgatcg aggagcgccc ggtggcggtc gccggcgcac gccggagcgc catgcccccg
     6841 agccgcccgc gcccgctgaa ggacgtctcg ctcgaccgca cggagcgcct gcgtaccggc
     6901 atcggcgagc tcgaccgggt cctgggtggc ggcgtcgtgc ccggctcgct ggcgctgatc
     6961 ggcggggatc cgggcgtcgg gaagagcacg gtgctgttgc aggcggcgcg cgcgctggcg
     7021 gaagcggcgc cgcccgtgct ctacgtctcg ggcgaggaat cggctgcgca ggtgaagctc
     7081 agggcggacc gcctcggcgt caccaccgac ggtctcttgc tctggtcgga gaacgatctc
     7141 tcggcggtcc aggccgagct ggacacggta aagccgcggg cgctcgtggt ggactcgatc
     7201 cagacggtgt tcctgcccga gctggagtcg gcccccggca gcgtcgcaca ggtgcgcgag
     7261 tgcggcgcgc gcctcatgac gcttgccaag ggccgcgggc tggcgacctt catcgtcggt
     7321 cacgtaacca aggacggctc gctggccggt ccccgcgtgc tcgagcacct ggtggacacg
     7381 gtgctctact tcgagggcga gcagcaccac gcctaccgcg tgctccgtgc ggtgaagaac
     7441 cggttcggct cgaccaacga gatcggcgta ttccagatgg gcgagcgcgg cctgatggag
     7501 gttcccaacc cctcgggctt cttcctggcc gagcgcccca aggacgcgcc ggggtcggtg
     7561 atcgtggccg gcctggaggg cacgcgcccg ctgctgctcg agctgcaggc gctggtcacg
     7621 ccggcctcgt tcggcacgcc gcggcgcacg gtgatcggcg ccgactacaa ccgcacgtgc
     7681 ctgctgctgg cggtgctcga gaagcgtgcc ggcgttccgc tgggcaccca ggacgtcttc
     7741 gtcaacgtcg cgggcggcgc ccgcatcgtc gagcccgcgg ccgacctggg tgtcgtcgtc
     7801 gccgcagctt cgagctatct ggaccggccg gtgcggggtg acgtcgtggt ggtcggcgag
     7861 gtcgggctca ccggagaggt ccgtgcgatc accgggctcg aggcgcggct gcgcgaggcc
     7921 gccgcgctcg gctttcgcga ggccgtcgtg ccccagagca acctcgctga gcagccgcgg
     7981 ctgcccctgg aggcgcgcgg ggtgacgacc gtgcacgcgg ccctcgacct gctggtgggc
     8041 tgaggcgatg cgatccggcc tgggcgctac gaggacaggt gctcggagaa tgccggggcc
     8101 tccgcgggcg gctcgagccg tgcgcgacgt cgccgtcctc gtgccggcgg cgggcgcggg
     8161 cgcgcgaatg ggcggccgca cgccgaagca gttcctcgcg ctcggtggcg cgccgatcct
     8221 cgcggtgacg gttcgccgt
//
LOCUS       DATIAZ010000275         4529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1261455, whole genome shotgun sequence.
ACCESSION   DATIAZ010000275 DATIAZ010000000
VERSION     DATIAZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261455"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VLF19_09775"
     CDS             complement(<1..234)
                     /locus_tag="VLF19_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HSE93583.1"
                     /translation="MGRRALGRPRFRFFAGKGGVGKTTCAAAAAVGAAERGRRVLLVS
                     TDPAHSLRDVLTVAPDSGEAAAGRRSGPRARLGP"
     gene            complement(216..491)
                     /locus_tag="VLF19_09780"
     CDS             complement(216..491)
                     /locus_tag="VLF19_09780"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HSE93584.1"
                     /translation="MIILDSLLFGGLRFVLGKIAQAVDAELDDAERLREELLAAQMRV
                     ELGEMSAEDFAALEREILTRLRELRGAESPGRMRVTGVEARAWDDEP"
     gene            complement(495..1370)
                     /locus_tag="VLF19_09785"
     CDS             complement(495..1370)
                     /locus_tag="VLF19_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HSE93585.1"
                     /translation="MKPKIERPARGRSGSRRTDAGRRRRATARTAARVRPDADDLGKY
                     VYCVIGALQPMRFGPIGIGNRPAEVHTINYKDIAAVVSDTPIEVYDPTRENVLAHERV
                     NESVMRTNTVIPMSFGTVFKTRDDIVELLRGAYEAFHDVLVKMADKLEFGLKVLWDRD
                     VMVRETERQHEDIRRLKNEIAAHKGSTYFARVQYGRLVDAALQELSERYVGDIFTQLR
                     DVCVASRANKPIGDKMIVNAAFLVARDQEAAFDTRVKRIGAQYDKLTFKYTGPWPPYN
                     FVNIRLKLERAAEPK"
     gene            complement(1367..1786)
                     /locus_tag="VLF19_09790"
     CDS             complement(1367..1786)
                     /locus_tag="VLF19_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93586.1"
                     /translation="MATGPLVLIVDDFQDNREMYAMFLEHSGYRVAEAATGFEALERA
                     VELLPDVVVMDLSLPGLDGWEATRRLKADPRTKHIPVLALTSHALEGFSEGAKAAGCD
                     TFVTKPCLPDQLVAEIRALLRDRHPKPRTSTPRARET"
     gene            complement(1786..3930)
                     /locus_tag="VLF19_09795"
     CDS             complement(1786..3930)
                     /locus_tag="VLF19_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE93587.1"
                     /translation="MSRAVARPRSRVVRPDAGDRLALVEFLLGCDDAADCAQRAVDWL
                     GTHAGARQVLCLAADEASTRLTALAARGIAPSRAARVASLLDRNDHPFAATIAAGGGP
                     AIVSPNGRPAPMPLGSGPLLTVPLPPPAHLRSDGRPGLLVVKPVTADLERETRWLAEL
                     LGPKLARLRGATLTAAPETLERERTLLDTIINAVPDPVLLTDPEGRMILANARAEMLF
                     ATTERESEGRRRAVALNNMLFSAALAGRTIDATEPSRRELLLVDPTEGSDLLFELLSA
                     VVGDSRQAPRVLSVLRNVTDLRTAVDELEENLRKLRLAEADARAERDRLDLIIDAVAD
                     PIVVSDPAGAIALMNAPAERLFTAAPEASNEATLRVQSNDAHFSSFVSNLMFAGDTLR
                     YSGGVSLTEPTTGRPLPVEAVAGKVLSGHGELMGVVTILHDQTEALEKQRLYEQVKRA
                     SEQLEERVREATGELVHQNELLRRQAIQLEHASALKSQFLANMSHEFRTPLNAILGYT
                     SILLQGVSGELTPLQRRSLTRVDSSGRHLLALIDDILDISRIEAGKMPLHVTEFTLAG
                     LVAEVMAEVEPIIARTTLTVTKALAPDLPAVRSDRQKVKQIVLNFLTNALKFTLEGWV
                     KVTAGVDARADRFLIAVADTGIGIAEHDQAKVFEDFSQADNSSTRAYGGAGLGLAICR
                     RLASMLGGQITLQSKLGRGSTFTLSLPGRLAT"
     gene            complement(4055..4270)
                     /gene="gvpA"
                     /locus_tag="VLF19_09800"
                     /pseudo
     CDS             complement(4055..4270)
                     /gene="gvpA"
                     /locus_tag="VLF19_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_component: GO:0033172 - gas vesicle shell [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle structural protein GvpA"
     gene            complement(4260..>4529)
                     /locus_tag="VLF19_09805"
     CDS             complement(4260..>4529)
                     /locus_tag="VLF19_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93588.1"
                     /translation="ELLTIRIRLIICSIDKAQEIGLDWWKHDRHLAPGRLGAANTDDL
                     SQQLRALERKVALLSGERPLTAAAVGHAAGPRAPKSRRRRAPDGR"
BASE COUNT          618 a         1567 c         1662 g          682 t
ORIGIN      
        1 ggggccgagc ctggcccggg ggcccgagcg ccggccggca gcggcttcgc ccgagtccgg
       61 ggcgacggtc agcacgtctc gcagcgagtg cgcgggatcg gtggacacga gcagcacgcg
      121 acggccgcgc tcggcggcgc ccaccgccgc cgcggccgcg caggtggtct tgccgacgcc
      181 gcccttgccc gcgaagaagc ggaaccgagg gcggcctagg gctcgtcgtc ccaggcgcgg
      241 gcctccacgc cggtcacgcg catgcgcccg ggcgactcgg ccccccgcag ctcgcgcagg
      301 cgggtcagga tctcgcgctc gagggccgcg aagtcctcgg ccgacatctc gccgagctcc
      361 acgcgcatct gcgcggccag cagctcctcg cgcagccgct ccgcgtcgtc cagctccgcg
      421 tccacggcct gggcgatctt gcccagcacg aagcgcaggc caccgaagag cagcgaatcg
      481 aggatgatca cgcgctactt gggttccgcc gcgcgctcga gcttcaggcg gatgttcacg
      541 aagttgtagg gaggccaggg gccggtgtac ttgaacgtca gcttgtcgta ctgcgccccg
      601 atcctcttga cacgcgtgtc gaaggcggct tcctggtcgc gcgcgacgag gaaggccgcg
      661 ttcacgatca tcttgtcgcc gatgggcttg ttggctcgcg acgccacgca cacgtcgcga
      721 agctgggtga agatgtcgcc cacgtaccgc tcggacagct cctgcagcgc ggcgtccacc
      781 aggcggccgt actgcacgcg cgcgaagtag gtcgagccct tgtgcgcggc gatctcgttc
      841 ttcagccgcc ggatgtcctc gtgttgccgc tcggtctcgc ggaccatcac gtcgcggtcc
      901 cagaggacct tgagcccgaa ctcgagcttg tcggccatct tcaccaggac gtcgtggaag
      961 gcctcgtacg cgccgcggag cagctcgacg atgtcgtcgc gcgtcttgaa cacggtgccg
     1021 aacgacatgg ggatcacggt gttggtgcgc atgacgctct cgttgacgcg ctcgtgggcc
     1081 agcacgttct cgcgcgtggg gtcgtacacc tcgatcggcg tgtcggacac gacggcggcg
     1141 atgtccttgt agttgatcgt gtggacctcg gccggcctgt tgccgatgcc gatcggcccg
     1201 aagcgcatgg gctgcagcgc gccgatgacg cagtagacgt acttgccgag gtcgtcggcg
     1261 tccggcctca cacgcgcggc cgtcctggcg gtcgcccgcc gccggcgccc ggcgtccgtt
     1321 cgccgggacc cggaccggcc ccgcgccggc cgctcgatct tcggcttcat gtctccctcg
     1381 cgcgcggggt ggaggtgcgt ggcttcgggt gccggtcacg cagcaacgcc cggatctcgg
     1441 ccacgagctg gtccgggaga cagggcttgg tcacgaacgt gtcgcagccc gcggccttgg
     1501 cgccctccga aaagccctcc agcgcgtggc tggtgagcgc cagcaccgga atgtgcttcg
     1561 tgcgcgggtc ggccttcagg cggcgtgtcg cctcccagcc gtcgagtccg ggcagggaga
     1621 gatccatgac gacgacgtcg ggcaggagct cgacggcccg ttccagcgcc tcgaagccgg
     1681 tcgcggcctc ggccacccgg tagccggagt gctccaggaa catcgcgtac atctcgcgat
     1741 tgtcctggaa gtcgtcgacg atgaggacga gtggtccggt cgccatcagg ttgccagtct
     1801 ccccggcagg ctcagcgtga acgtcgagcc ccgtccgagc ttgctctgca gggtgatctg
     1861 gccgccgagc atcgaggcca ggcggcggca gatcgccagc cccaggccgg cgccgccgta
     1921 agcacgggtg gacgagttgt cggcctggct gaagtcctcg aagaccttcg cctgatcgtg
     1981 ctcggcgatg ccgatgccgg tgtcggccac ggcgatcagg aagcggtcgg cccgcgcgtc
     2041 cactcccgcc gtcaccttca cccacccttc cagcgtgaac ttcagggcgt tggtgaggaa
     2101 gttgagcacg atctgcttca ccttctgccg gtcgctccgg acggcgggca ggtcgggcgc
     2161 cagcgccttg gtcacggtca gggtggtgcg ggcgatgatg ggctcgacct cggccatgac
     2221 ctccgcgacc agcccggcga gcgtgaactc ggtgacgtgg aggggcatct tgccggcctc
     2281 gatccgggaa atgtcgagga tgtcgtcgat cagcgcgagc aggtgccggc cggacgagtc
     2341 cacccgcgtc aggctccggc gctgcagggg cgtcagctcc ccggagacgc cctgaagcag
     2401 gatggaggtg tagccgagga tcgcgttcag cggcgtgcgg aactcgtgcg acatgttggc
     2461 gaggaactgt gacttgaggg ccgaggcgtg ctcgagctgg atcgcctgcc ggcgcagcag
     2521 ctcgttctgg tgcaccagct ctcccgtcgc ctcacgcacc ctctcctcga gctgctcgga
     2581 ggcgcgcttg acctgctcgt acagccgctg cttctcgagc gcctcggtct ggtcgtggag
     2641 gatcgtcacc acccccatca gctcgccgtg acccgaaagc accttgccgg ccacggcctc
     2701 caccggcagc ggccgccccg tcgtcggctc ggtcaggctg acgccgccgc tgtagcggag
     2761 cgtgtccccg gcgaacatga ggttcgagac gaacgacgag aagtgggcgt cgttggactg
     2821 cacccgcagg gtcgcctcgt tggacgcctc cggcgccgcg gtgaagagcc gttcggccgg
     2881 ggcgttcatc agcgcgatgg cgccggcagg atccgacacc acgatgggat cggcgacggc
     2941 gtcgatgatg aggtcgaggc ggtcgcgctc ggcccgggcg tcggcctcgg cgagccggag
     3001 ctttctcagg ttctcctcca gctcgtcgac cgccgtgcgg agatcggtca cgttgcgcag
     3061 caccgacagg acgcgggggg cctggcgcga gtcgccgacg accgcgctga gcagctcgaa
     3121 gaggaggtcc gagccctcgg tcgggtccac gagcaggagc tcgcggcgcg acggctccgt
     3181 ggcgtcgatc gtgcgcccgg cgagcgcggc ggagaacagc atgttgttga gcgcgacggc
     3241 ccgccgccgt ccctcgctct cgcgctccgt cgtcgcgaag agcatctcgg cccgcgcgtt
     3301 ggccaggatc atgcggccct cggggtccgt cagcagcacg gggtcgggca ccgcgttgat
     3361 gatcgtgtcg agcagcgtcc gctcgcgctc gagcgtctcg ggcgcggcgg tcagcgtcgc
     3421 gccgcgaaga cgggcgagct tggggccgag gagctcggcg agccagcgcg tctcgcgctc
     3481 gaggtcggcc gtgaccggct tgaccacgag cagaccgggc ctcccgtcgg aacgcaggtg
     3541 cgccgggggg ggcagcggga cggtgaggag cggaccgctg ccgagcggca tgggcgccgg
     3601 gcggccgttc ggcgacacga tcgccggccc gccgccggcg gcgatcgtgg ccgcgaaggg
     3661 gtggtcgttc cggtcgagga gggacgctac ccgcgccgca cgcgacgggg cgatgccgcg
     3721 cgccgcgagg gcggtgagcc gcgtgctcgc ttcgtcggcc gccaggcaaa gcacctgccg
     3781 ggcgccggcg tgcgtgccga gccagtccac cgcccgctgc gcgcagtcgg cggcgtcgtc
     3841 acagccgagc aggaactcca cgagggccag gcgatccccg gcgtccggcc ggaccaccct
     3901 ggatcgcggg cgagcgacgg cgcgcgacac ggcgtcagac gcgggcgacg cggcgccgat
     3961 gcctcacccc gactccatcg gttccggagg ccgcgagctg cgtgcgcagt gccgcgttct
     4021 ccgcggcgag ggactcctgg ctgcggctgt cgacggcggc ggggcgcgag gcgggcgtga
     4081 tcgagccgac ggcctcggag tacttcaggt acgtctcgat cgatgcgacg acgacgcggg
     4141 cctcgaccgt gatcaggtcg atgccgacga gcgagacgcg gacccacgca tcgatgacga
     4201 tgcccttgtc caggacccga tcgaggacgt cgatcaggct ggtgccgccg gatgttcgtt
     4261 caacggccat cgggtgccct cctacgacgg ctcttcggtg cgcgcggtcc ggccgcgtgg
     4321 ccgacggcgg cggcggtcag cgggcgctcg ccggacaaga gcgcgacttt gcgctcgagc
     4381 gcgcgcagct gctgggagag gtcatcggtg ttcgccgccc cgagccgacc cggcgcgagg
     4441 tggcggtcgt gtttccacca gtccagcccg atctcctgcg ccttgtcgat cgagcagatg
     4501 atcaaccgga tgcggatcgt gagcagctc
//
LOCUS       DATIAZ010000276         3625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1130693, whole genome shotgun sequence.
ACCESSION   DATIAZ010000276 DATIAZ010000000
VERSION     DATIAZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130693"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /locus_tag="VLF19_09810"
     CDS             <1..2256
                     /locus_tag="VLF19_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSE93589.1"
                     /translation="ASARAALTTARDRAVADLAALSSAREQLAAALAGHQDELAEVEA
                     RLAEGRVALAARRSSLEALVALERAREGYGSGVRAMFAEHGQPRVAGIVGTVADLLDV
                     PPDLERAVEAVLGERLEWIVVGRFEQARTAVAFLAEHSAGAATFLPLETLGDQSGEPR
                     GTPPLSSSARWVVQQVAARAPRLLEYLLGDVAIVDHLDQAEALWRRNGVVATYVTPQG
                     EVLTPMGRLRGGAGNGAAERSLLARKRQIRELEDEVRGIDAGVAAAQATAARLGDEVA
                     ALRARIAESEQALSIRRAERLAGEKDLEQAGREHERVVRHAETVRLEAGQVGRESDET
                     AAMLTRLEQHIAAAREAEAAHETSMAALREAIDGAQQRETALVTELTACRVEVASVAE
                     RVEALGRETARIDQMEADLDDRLAQARTRRTQLADRRVWLAEERDRTDEAARQVAVAR
                     DRIDGDVRVAAERNEALVAELRAVEQDARELDGAVRTLVAGIHELELAATERRVRREE
                     LALEAARSFGVDETALRGRYDPARDLAAFRVRLLELEQRLTAIGPVNLVADEEYRELD
                     ERLTFLKTQHDDLTASIKDLDKALRGMTRTAQERFTHAFHEINREFSRIFERLFEGGR
                     AELHLVVAEEDGDPLDTGVELMAQPRGKRLQSVTLLSGGERALTGLALLFAIFYYRPS
                     PFCVLDEVDAPLDDANIHRFLRVLRELTSQTQFLVITHNRRTMEAADILYGVTMEEPG
                     LSKLVSVKLGA"
     gene            2296..3195
                     /locus_tag="VLF19_09815"
     CDS             2296..3195
                     /locus_tag="VLF19_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HSE93590.1"
                     /translation="MASLGPYLRELRERRGLSLEEMVRATRVASRYLEALEAEDLRAL
                     PAPTFVRGFIRAWCQTVGVDATEALARYQELSGAPMPGATRPAAEVAGEHELPAGSRN
                     RGAVLVSFVLLVILGLALFAVTLVLQSGRDRAERRAGSPPGIPAAAAPPASTSGTHGG
                     GSPSDEGAAATKESPSASGRRPEPAAAPQAAGRAPVAVPGSAQASPSPGGDSAGTLTP
                     RYRLVARVSEPTWIRVRMEEGRATEETIPAGQTREWVSNAPFVLTVGNAGGVTLELNG
                     RALPSLGARGAVISRLVIPPAAR"
     gene            3192..>3625
                     /locus_tag="VLF19_09820"
     CDS             3192..>3625
                     /locus_tag="VLF19_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HSE93591.1"
                     /translation="MKFLSGLGDRLSAGLRRSRDLLASELKSVFEPDRPIDETLWVEL
                     EEVLIGADLGATLAADFVNRAREEVMFGTVTRADQLRPLLRRFLSDVLAAAAQPLDLE
                     HRPTVILVLGVNGSGKTTTCAKLAASLKDGGRQVLLAAADTFR"
BASE COUNT          491 a         1293 c         1374 g          467 t
ORIGIN      
        1 gcgtcggcgc gcgcggcgct cacgacggcc cgtgaccgcg ccgtcgccga tctcgccgcg
       61 ctctcgagcg cccgcgagca gctcgcggcc gccctggccg ggcaccagga cgagctcgcg
      121 gaggtcgagg ccaggctggc cgaggggcgg gtggcgctgg cagcgcggcg ctccagcctc
      181 gaggcgctcg tggcgctcga gcgcgcccgg gagggctacg gctccggcgt gcgcgccatg
      241 ttcgcggagc acggtcagcc gcgcgtcgca ggaatcgtgg gaaccgtcgc cgatctgctc
      301 gacgtcccgc cggacctcga gcgcgcggtg gaggcggtac tgggtgagcg gctcgagtgg
      361 atcgtcgtcg gtcgcttcga gcaggcccgg accgccgtcg ccttcctggc cgagcacagc
      421 gccggcgccg ccacgttcct gccgctggaa acgctcggtg atcagagcgg cgaaccccgt
      481 gggacacctc cgctgtcctc ctcggcccgc tgggtggtgc agcaggtggc cgcccgcgcg
      541 ccccgcttgc tcgagtacct cctgggcgac gtcgcgatcg tggaccacct cgaccaggcc
      601 gaggcgttgt ggcgccgcaa cggcgtcgtg gcgacctacg tcacgcctca gggcgaggtg
      661 ctgaccccga tggggcggct gcgaggaggc gccggcaacg gggccgccga gcgctcgctg
      721 ctcgcacgca agcgccagat ccgcgaactc gaggacgagg tgcgcggcat cgacgcggga
      781 gtggctgccg cacaggccac cgcggcgcgg ctcggtgacg aggtggcggc gctgcgcgcg
      841 cggatcgccg agagcgagca ggccctgagc atccgccggg ccgagcgatt ggccggcgag
      901 aaggatctcg aacaggccgg acgcgagcac gagcgcgtgg tccgccacgc cgagaccgtg
      961 cgcctggagg ccggccaggt ggggcgcgag tccgacgaga cggccgcgat gctgacgcgg
     1021 ctggagcagc acatcgccgc cgcccgcgag gccgaggccg cccacgagac gagcatggcc
     1081 gccctgcgcg aagccatcga cggcgcgcag cagcgcgaga cggcgctcgt caccgaactg
     1141 acggcgtgcc gggtggaggt cgcgtccgtg gccgagcgcg tggaggcgct gggtcgggag
     1201 accgcgcgca tcgaccagat ggaggccgac ctcgacgacc ggctggccca ggcgcgcacg
     1261 cgccggacgc agctcgccga tcggagggtg tggctcgccg aggagcgcga ccggaccgac
     1321 gaggcggccc gacaggtggc cgtggcgcgc gaccgcatcg atggggacgt gcgggtcgcc
     1381 gccgagcgca acgaggcgct cgtcgcagag ttgcgcgccg tggagcagga cgctcgcgag
     1441 ctcgacgggg ccgtccggac gctggtcgcg ggcatccacg agctcgagct ggcggcgacg
     1501 gagcgacgcg tgcggcgcga ggagctcgcg ctggaggcgg cgcgaagctt cggcgtggac
     1561 gaaaccgccc tgcgcgggcg ctacgacccg gcgcgcgacc tggcggcctt ccgggtgcgt
     1621 ttgctcgagc tggagcagcg gctcaccgcc atcggcccgg tgaacctcgt ggccgacgag
     1681 gagtaccgcg agctcgacga gcggctgacc ttcctcaaga cccagcacga cgacctgacg
     1741 gcgtcgatca aggacctgga caaagcgctg cgcggcatga cgcgcacggc gcaggagcgc
     1801 ttcacccacg ccttccacga gatcaaccgg gagttctcgc gcatcttcga gcgcctcttc
     1861 gagggcggcc gggccgagct gcacctcgtc gtggccgagg aggacggcga cccgctcgac
     1921 accggcgtgg agctgatggc gcagccgcgg ggcaagcggc ttcagtccgt gacgctgctc
     1981 tcgggcggcg agcgggccct gaccggcctc gcgctgctgt tcgccatctt ctactaccgg
     2041 ccgagcccgt tctgcgtgct cgacgaggtc gacgcgccgc tcgatgacgc caacatccat
     2101 cgtttcctgc gcgtgctccg cgagctcacg agccagacgc agttcctggt gatcacccac
     2161 aaccgccgaa cgatggaagc cgccgacatc ctctacggcg tcacgatgga ggagcccggg
     2221 ctctcgaagc tggtctccgt caagctcgga gcctgacaga ctccgggaca tggattagac
     2281 tcgaagagcg tgcctatggc ttcgctcggc ccgtatctcc gcgagctccg tgagcgccgg
     2341 ggcttgagcc tcgaggagat ggtgcgcgcg acccgtgtgg ccagccgcta cctggaggcc
     2401 ctcgaggccg aggacctgcg agctctgccg gcgccgacct tcgtgagggg attcattcgc
     2461 gcctggtgtc agaccgtcgg cgtcgatgcg accgaggcgc tcgcgcgcta ccaggagctg
     2521 agcggggcgc cgatgccggg ggccacccgg cccgcggcgg aggtcgccgg cgagcacgag
     2581 ctcccggccg ggtcgcggaa ccggggcgcc gtgctggtga gcttcgttct gctggtcatc
     2641 ctcggcctcg ccctgttcgc ggtgaccctc gtgctccagt cgggtcggga ccgcgcagag
     2701 cggcgcgccg ggtcaccgcc cgggatcccc gcggccgccg cgcccccggc atccacgtcc
     2761 ggaacccatg gaggtgggtc gccgtcggac gagggcgccg ctgccaccaa ggagagccca
     2821 tcggcgtccg gccgccgtcc cgagcctgcc gccgcgcccc aggctgcggg acgcgcaccg
     2881 gtggcggtac ccggctcggc gcaggcctcg ccctctcccg ggggggatag cgcggggacc
     2941 ctgaccccgc gctatcggct ggtggcgcgg gtgagcgagc cgacgtggat ccgggtgcgc
     3001 atggaggagg gccgcgcgac cgaggagacg atccccgccg gccagacgcg ggagtgggtg
     3061 tcgaacgccc cattcgtgct gaccgtcggc aacgcgggcg gcgtcacgct ggagctgaac
     3121 ggccgcgcgt taccttcgct gggggcccgg ggcgccgtca tctcgcggct ggtcatcccg
     3181 ccggccgcac ggtgaagttc ctctccggac tgggcgaccg cctgagcgcc ggcctcaggc
     3241 ggtcacgtga tctcctcgcc tccgagctca agagcgtctt cgagccggat cggccgatcg
     3301 acgagaccct ctgggtcgag ctcgaggaag tgctgatcgg cgcagacctc ggcgccacgc
     3361 tggcggccga tttcgtgaat cgcgcgcgcg aggaggtcat gttcggcacg gtcacccgtg
     3421 ccgaccagct ccggccgctc ctccgtcgct tcctgagcga cgtgctggcg gcggccgcgc
     3481 agccgctgga tctggagcac cggccgacgg tgatcctcgt gctcggggtc aacggctccg
     3541 gaaagaccac gacgtgtgcg aagctcgccg cctcgctcaa ggacggcggg cggcaggtgc
     3601 tcctggccgc cgccgacacg ttccg
//
LOCUS       DATIAZ010000277         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1658611, whole genome shotgun sequence.
ACCESSION   DATIAZ010000277 DATIAZ010000000
VERSION     DATIAZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658611"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VLF19_09825"
     CDS             <1..1414
                     /locus_tag="VLF19_09825"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93592.1"
                     /translation="GAHVMATHWRNRVPMRQRAALALALLLALDLSGCATGPRTAARP
                     PDAAVTRVPDLPRPADPPAPPRSVDSTPAPVVPTPAPAPAVPGGPGPDGAARARAEFE
                     EGQALIIRGELARAIASLRSALRLQPDFAEARVSLGLALYGAGDLDGAIDELRALLRT
                     RPDLVPARLVLASALMARRDWSAARAELEEALARRPDSLQAYYSLGFVRYALGDLAGA
                     IAAYRSVLARQPEHHDARYNLALVLRLAGRDAEATAEFVAAAEGGVPRAQYFAGAAYA
                     TGVGVERDLGAAIAWWFRASEHGVAQADEALAQMRQTAQGRGRSAPGERRAAEQAFLD
                     FRASLWGQFPDLARDGSDSAGTALLRSGRAGEAVPVLIREALALSEPAERLLERAYEQ
                     GADGVAPHDPRILGYFGTAAAEGRVRARLALARIHALGLGVPADVDRAIGLLEATPHE
                     DAQRLLRELSAASGAIPARP"
     gene            1461..>2245
                     /locus_tag="VLF19_09830"
     CDS             1461..>2245
                     /locus_tag="VLF19_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HSE93593.1"
                     /translation="MLPATVIALAATLVAGAISALGGPRVTALEWSAYDRWLRGREGA
                     PSRPPLVVVVRDPASEARFGRDGWDRALLARVITNLARAGAGVIGVDVGLATPGVGGR
                     GGPAADALLSEATAMAGNVVYPVPPAPPVPPLAQRARGGGHILTAPDADGVVRKVPLF
                     VSRDGAAVPAFGLALAAALRGTDADRPPVDGVGTREPGAARSGPVQIPVDRHGPSLVG
                     FSGSGFPRGTTVVPFSEVWTAIEERRLETLQRLVEDNLVLVAA"
BASE COUNT          233 a          851 c          873 g          288 t
ORIGIN      
        1 gggagcgcac gtcatggcca cccactggag aaacagagtt ccgatgcgcc agcgtgcggc
       61 gctcgccctc gcgctactgc tggcgctcga tctctccggc tgcgcgacgg gaccgaggac
      121 ggccgcgcgc ccgccggacg ctgccgtcac gcgcgtgccg gaccttccgc ggcccgccga
      181 cccaccggcg cccccccggt cggtggactc gacgccggcg cccgtcgtgc cgacgcccgc
      241 tccggccccg gcggtcccgg gggggccggg ccccgatggc gcggcgaggg cgcgagcgga
      301 gttcgaggag gggcaggcgc tcatcattcg cggcgagctg gcgcgagcga tcgcgtcgct
      361 ccgttcggcg ctgcgcctgc agcccgactt cgccgaggcg cgggtgagcc tgggtcttgc
      421 gctctacggt gcgggcgacc tcgacggcgc catcgacgag ctgcgcgcgc tcttgcggac
      481 gcggcccgat ctcgtgccgg cgcggctcgt gctcgcctcg gcgctcatgg cccggcgcga
      541 ctggagcgcc gcgcgcgccg agctggagga agcgctcgca cgccgcccgg actcgctcca
      601 ggcgtactac agcctcggct tcgtccgcta tgcgctgggg gacctcgccg gcgccatcgc
      661 ggcataccgg agcgtgctcg cgcggcagcc cgagcaccac gacgcgcgct acaacctcgc
      721 gctcgtgctc aggctggccg gccgcgacgc cgaggcgacg gctgagttcg tcgcggccgc
      781 cgagggcggg gtacccaggg cccagtactt cgcgggcgcc gcgtacgcga ccggtgtcgg
      841 cgtcgagcgc gacctcggcg ccgccatcgc gtggtggttc cgggcgtcgg agcacggcgt
      901 ggcccaggcg gacgaagcgc tggcccagat gcgccagacg gcgcagggca ggggtcgctc
      961 cgcccccggt gaacgccggg ccgccgagca ggcgttcctc gacttccgcg cctcgctctg
     1021 gggccagttc ccggacctcg cgcgcgacgg gagcgactcc gcgggcacgg cgctgctgcg
     1081 gtccggccgc gcgggtgagg ccgtgcccgt gctgatccgc gaggcgctgg cgctgagcga
     1141 gccggcggag cggctgctgg aaagggccta cgagcagggc gcggacggcg tcgccccgca
     1201 cgacccgcga atcctcggct acttcggcac cgccgccgcg gagggccggg tccgcgcccg
     1261 gctggcgctc gcgcgcatcc atgcgctcgg gctcggcgtg cctgccgatg tcgaccgcgc
     1321 catcggcctg ctcgaggcca cgccgcacga ggacgcgcag cgcctgctgc gcgagctctc
     1381 ggccgcgagc ggcgcgatcc cggcgcgtcc gtagctcggg gagcgccatc acgcttcgca
     1441 cgtcgcctcc gttgcgcgcg gtgttgccgg ccaccgtcat cgcgctcgcg gcgaccctcg
     1501 tggctggcgc catctccgct ctcggcgggc cgcgcgtgac cgcgctggaa tggtcggcct
     1561 acgaccgctg gctgcgcggg cgcgagggcg cgccgtcccg cccgccgctc gtggtcgtcg
     1621 tacgagaccc cgcgagcgag gcgcgcttcg ggcgcgacgg atgggatcgc gccctcctcg
     1681 cgcgggtcat cacgaatctg gctcgcgccg gcgccggcgt catcggggtg gacgtcgggc
     1741 tcgcgacgcc gggcgtcggg ggacgcggcg gcccggccgc cgacgcgctc ctgagcgagg
     1801 cgacggcgat ggccggcaac gtcgtctatc cggtcccgcc ggcgccgccg gtgccgccac
     1861 tcgcgcagcg cgccaggggc ggcgggcaca tcctgacggc gccggatgcc gacggcgtgg
     1921 tccggaaggt gccgctcttc gtgagtcgcg acggcgccgc cgtgcccgcg ttcggcctcg
     1981 cgctggctgc cgcgctgcgc gggacagatg ccgatcggcc ccctgtcgac ggcgtgggga
     2041 cgcgtgagcc gggggctgcg cgcagcgggc cggtgcagat ccccgtcgat cgacacggac
     2101 cgtcactcgt cgggttcagc ggcagcggct ttccccgtgg caccacggtc gtgccgttct
     2161 ccgaggtctg gacggcgatc gaggagcgcc ggctggagac gttgcagcgt ctggtcgagg
     2221 acaacctggt gctggtcgcc gcgga
//
LOCUS       DATIAZ010000278         4121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_869238, whole genome shotgun sequence.
ACCESSION   DATIAZ010000278 DATIAZ010000000
VERSION     DATIAZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869238"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..375
                     /locus_tag="VLF19_09835"
     CDS             187..375
                     /locus_tag="VLF19_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93594.1"
                     /translation="MVERRRPKASKDDPICPVCLQPIKASDKVRGRSDELIHESCDCT
                     GRPVPPRRPGSGRGDSPA"
     gene            complement(388..2391)
                     /locus_tag="VLF19_09840"
     CDS             complement(388..2391)
                     /locus_tag="VLF19_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSE93595.1"
                     /translation="MFYRYSRWDGSQRLDLDADALMSAMADDLLHDGDPWRALRRLMQ
                     QGMRGPEGQRMPGLRDMLERLRQRRQQRLDRYDLGSSLDDIKRKLDDVLATERAGIER
                     RLGESREGARRGDVPQELAKKFERLAARNTQALDKLPPDPAGRIRGLQNYDFVDPEAK
                     RKFDELLDSLRQQMLQPFMQGMQAALKNMTPGDLARMREMLQDLNRMLRERAEGGEPD
                     FEAFKQKWGQHFPGVESLDQLLEQMGRQMAMMQSLMQSLTPEQRGQLDEMMRSLLLQD
                     ERLEAAMRQLAANLNDLLPLDELAQRYPFQGDEEVTLAEAMRLMDELQQMDALERELR
                     GVRALEDLEGLDADRVETLLGEDAREDLERLRELAKRLEEAGYLERDGEELKLTARGI
                     RKIADKALRDIFARLARDRFGGHAIEQRGAGGDQTDETKAYEFGDAFLLDMKGTVLNA
                     VTRRGPGTPVRLAPGDFEVHRTELRTQAATVVMLDMSRSMLNNGYFLPAKKVALALSS
                     LIRGQFPRDALYIVGFSLYAREFKGEELPTLSWSEWNVGTNMHHGFMLARRLLARHKG
                     GNRQILMVTDGEPTAHMEGDVADFSYPPMRRTIEETLREVRRCTRESITINTFMLERS
                     PWLVSFVQQMTRINRGRAFFAAPDRLGEYLVVDYVTSRRRALR"
     gene            complement(2396..3787)
                     /locus_tag="VLF19_09845"
     CDS             complement(2396..3787)
                     /locus_tag="VLF19_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HSE93596.1"
                     /translation="MSQAKTVGDLKAGGWKRKTVKQELRDNLIARLRRGGSFLPGIVG
                     YGDTVVPQIENAILSGQDVIFLGERGQAKTRIARLLVGLLDEAIPVLAGCEINDDPLA
                     PICQACRERVAAEGDGAAVAWLPRDRRYGEKLATPDITIADLIGEVDPIKVAEGRYLA
                     DELTIHYGMLPRTHRGIFALNELPDLAERIQVGLLNIMEERDVQIRGYKVRLPLDLYV
                     VASANPEDYTNRGRIITPLKDRFGSQIRTHYPKRLDDEIAIMEAEQTAFAGDGLRSQC
                     PQYMKQIVAELTHLARQSQEISQRSGVSVRVTICNYENLLSSALKRAIRLGEPLAVPR
                     VSDLGALAASTAGKIELETTGEGGEDKLLAKLVQRAVLNVFGRSFNAAELEPVVGAFK
                     DGFAVQVGDAMSSADYLKQCEKVPPLRAAAAKLGASEPAAVAAAMEFVLEGLHLGKKL
                     NKDAQPGRARYKA"
     gene            3850..>4121
                     /locus_tag="VLF19_09850"
     CDS             3850..>4121
                     /locus_tag="VLF19_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93597.1"
                     /translation="MTPSASRLHETRVLRGICYVVVATVLFVVMSSIVKHLSRSLPVV
                     ELIWARTLGHLIFVVALFAPARGGWRLLGTQRPAIQVARSLLLLVST"
BASE COUNT          531 a         1440 c         1485 g          665 t
ORIGIN      
        1 taccggaccc gcccgtcatg gtacgccacg cgcgcgcggg gccgccagcc ccgcccggcg
       61 gcctgcggga tggccccgag cgccgccgag ccgccggacc ggcgactacg tagaaggacg
      121 gattgcgcat cgaggcccgg acctgtacag tggcgcgccg ccgatgtgcg cggcggcgga
      181 gggacgatgg tcgaacggag acggcccaag gcctcgaagg acgatccgat ctgtcccgtc
      241 tgcctgcagc cgatcaaggc gtccgacaag gtgcgcggcc gcagcgacga gctgatccac
      301 gagtcgtgcg actgcacggg gcggccggtc ccgccccgcc gccccggctc ggggcggggc
      361 gacagccccg cctgaggcgc ggttcgccta ccggagcgcg cggcggcgcg aggtcacgta
      421 gtcgacgacg aggtactcgc ccagccgatc gggcgcggcg aagaaggcgc ggccccggtt
      481 gatgcgggtc atctgctgca cgaacgacac cagccacggg ctccgctcga gcatgaaggt
      541 gttgatcgtg atcgattcgc gcgtgcagcg ccgcacctcg cggagcgtct cctcgatggt
      601 gcgtcgcatc ggcggatacg agaagtcggc gacgtcgcct tccatgtgcg cggtcggctc
      661 gccgtcggtg accatgagga tctggcggtt gccgcccttg tgccgggcga ggagccggcg
      721 cgcgagcatg aagccgtggt gcatgttggt gcccacgttc cactcgctcc acgacagcgt
      781 cggcagctcc tcccccttga actcgcgcgc gtagagcgag aagcccacga tgtagagggc
      841 gtcgcgcggg aactgcccgc ggatgagcga ggagagcgcg agggccacct tcttggcggg
      901 caggaagtaa ccgttgttga gcatcgagcg gctcatgtcg agcatcacga ccgtggccgc
      961 ctgcgtccgc agctccgtgc ggtggacctc gaagtcgccc ggcgccagac gtaccggcgt
     1021 gccggggccc cggcgggtca ccgcgttcag caccgtgccc ttcatgtcga gcaggaaggc
     1081 gtcgccgaac tcgtaggcct tggtctcgtc cgtctggtcg ccgccggcgc cgcgctgctc
     1141 gatggcgtgg ccgccgaagc ggtcgcgggc cagccgcgcg aagatgtcgc gcagcgcctt
     1201 gtcggcgatc ttccggatgc cgcgcgcggt gagcttgagc tcctcgccgt cgcgctcgag
     1261 gtagccggcc tcctccaggc gcttcgccag ctcgcgcagg cgctcgaggt cctcgcgggc
     1321 gtcctcgccg agcagtgtct ccacgcgatc ggcgtccagg ccctcgaggt cctccagcgc
     1381 ccgcacgccg cgcagctcgc gctcgagcgc gtccatctgc tgcagctcgt ccatgaggcg
     1441 catggcttcg gcgagggtga cctcctcgtc gccctggaac gggtagcgct gggccagctc
     1501 gtcgagcggc agcaggtcgt tgaggttggc ggcgagctgg cgcatcgccg cctcgaggcg
     1561 ctcgtcctgc aggagcagcg agcgcatcat ctcgtcgagc tggccgcgct gctcgggcgt
     1621 gaggctctgc atgagcgact gcatcatggc catctggcgg cccatctgct cgaggagctg
     1681 gtcgaggctc tcgacgccgg ggaagtgctg gccccacttc tgcttgaagg cctcgaagtc
     1741 cggctcgcca ccctcggcgc gctcgcgcag catgcggttg aggtcctgga gcatctcgcg
     1801 catgcgcgcc aggtcgcccg gcgtcatgtt cttcagcgcc gcctgcatgc cctgcatgaa
     1861 cggctggagc atctgctgcc gcagcgagtc gagcagctcg tcgaacttcc gcttcgcctc
     1921 cgggtcgacg aagtcgtagt tctgcaggcc gcggatgcgg cccgccgggt cgggcggcag
     1981 cttgtcgagt gcctgcgtgt tacgggcggc gagccgctcg aacttcttcg ccagctcctg
     2041 gggcacgtcg ccgcgcctgg cgccctcgcg ggactcgccg agccgccgct cgatgccggc
     2101 gcgctccgtc gccagcacgt cgtcgagctt gcgcttgatg tcgtcgaggc tcgagccgag
     2161 gtcgtaccgg tcgagccgct gctgccgccg ctggcggagg cgctcgagca tgtcgcggag
     2221 gccgggcatc cgctggccct cggggccacg catgccctgc tgcatgaggc ggcgcagggc
     2281 gcgccacggg tcgccgtcgt gcaggaggtc gtcggccatc gccgacatca gcgcgtcggc
     2341 gtcgaggtcg agccgctgcg agccgtccca gcgcgagtag cggtagaaca cgccgtcagg
     2401 ccttgtagcg cgcgcggccg ggctgggcgt ccttgttgag cttcttgccg aggtgcaggc
     2461 cctcgagcac gaactccatg gccgcggcga cggcggcggg ctcggaggcg ccgagcttgg
     2521 cggcggccgc gcgcagcggc ggcaccttct cgcactgctt caggtagtcg gccgacgaca
     2581 tcgcgtcgcc gacctgcacc gcgaagccgt ccttgaaggc gccgacgacc ggctcgagct
     2641 cggcggcgtt gaaggaccgg ccgaagacgt tgagcaccgc ccgctgcacg agcttggcca
     2701 gcagcttgtc ctcgcccccc tcgcccgtcg tctccagctc gatcttgccg gcggtggagg
     2761 cggcgagcgc gcccaggtca ctcacgcgtg gcacggcgag cggctccccc aggcggatgg
     2821 cgcgcttgag cgcgctcgag agcagattct cgtagttgca gatggtcacg cgcaccgaga
     2881 cgcccgagcg ctggctgatc tcctgcgact gccgcgccag gtgtgtcagc tcggcgacga
     2941 tctgcttcat gtactgcggg cactggctgc gcaggccgtc gccggcgaag gcggtctgct
     3001 cggcctccat gatcgcgatc tcgtcgtcca ggcgcttggg gtagtgcgtg cggatctgcg
     3061 agccgaagcg gtccttgagc ggggtgatga tgcgtccgcg gttggtgtag tcctcggggt
     3121 tggcgctggc caccacgtag aggtcgagcg gcagccgcac cttgtagccg cggatctgca
     3181 cgtcgcgctc ctccatgatg ttgagcaggc ccacctggat gcgctcggcc aggtccggca
     3241 gctcgttgag cgcgaagatg ccgcggtggg tgcgcggcag catgccgtag tggatcgtca
     3301 gctcgtcggc gaggtaccgg ccctcggcga ccttgatggg gtcgacctcg ccgatcaggt
     3361 cggcgatggt gatgtccggt gtggccagct tctcgccgta ccgccggtcg cgcggcagcc
     3421 acgccaccgc ggcgccgtcg ccctcggcgg cgacgcgctc gcgacacgcc tggcagatcg
     3481 gcgccagcgg atcgtcgttg atctcgcagc cggcgagcac ggggatcgcc tcgtcgagca
     3541 atccgacgag cagccgcgcg atgcgcgtct tggcctggcc gcgctcgccg aggaagatga
     3601 cgtcctggcc gctgaggatg gcgttctcga tctgcggaac cacggtgtcg ccgtagccca
     3661 cgatgccggg caggaacgac ccgccacgcc gcaatcgcgc gatgaggttg tcgcgcagct
     3721 cctgcttgac cgtcttccgc ttccaaccgc ccgccttcag gtcgccgacc gtcttcgcct
     3781 ggctcatgtg tcggccacta tgcgaccccg gccacggggt gtcaagccga gtgcgataag
     3841 ctgggcgcga tgacgccttc cgcctcgcgg ctgcacgaga cccgcgtgct gcgcgggatc
     3901 tgctacgtgg tcgtggccac ggtgctcttc gtcgtcatga gcagcatcgt gaagcacctg
     3961 agccggtcgc tgcccgtcgt cgagctgatc tgggcgcgca cgctgggcca cctgatcttc
     4021 gtggtcgcgc tcttcgctcc ggcgcgcggc ggctggcgcc tgctcggcac gcagcggccg
     4081 gcgatccagg tcgcgcgatc cttgctgctg ctcgtctcca c
//
LOCUS       DATIAZ010000279         7545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_343452, whole genome shotgun sequence.
ACCESSION   DATIAZ010000279 DATIAZ010000000
VERSION     DATIAZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343452"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VLF19_09855"
     CDS             <1..325
                     /locus_tag="VLF19_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93598.1"
                     /translation="RTAPGLPEEVRRLFVTATEVSVHGHLRVQAAFQRHVDNAVSKTI
                     NLPENTDQESVAEAFREGWRLGLKGVTVYRSGSKGDQVVTLGVGEDATAREFFAKCDP
                     GACRV"
     gene            complement(432..791)
                     /locus_tag="VLF19_09860"
     CDS             complement(432..791)
                     /locus_tag="VLF19_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSE93599.1"
                     /translation="MAPVLLGMEVEGYRMFVVKIVLVVLILAAAPACAQDSKALVAEG
                     QRVFRVQGCYGCHMVGAFGTPIGPDLSHVGAKYGQPYLLRWLRDPEVQRPSAHMPKIE
                     LTGAEVEALAAYLAALQ"
     gene            926..1501
                     /locus_tag="VLF19_09865"
     CDS             926..1501
                     /locus_tag="VLF19_09865"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HSE93600.1"
                     /translation="MTGSQTETAGPYPKELERDVLLKDGERLRIRPIRSDDAQRLIEL
                     YDRFSRHTAYQRFFTVMRRLPPDWARMLATVDYRRRLALVAERETPGGPELVGVARYE
                     PTDRDDTAEVAFAIQDGWQNRGLGTALFADLLDAAEARGIRRFVAYVLADNIRMLDLI
                     TRFGAVERRTLEQGVVELVFTPRGAPTARVH"
     gene            complement(1535..2179)
                     /locus_tag="VLF19_09870"
     CDS             complement(1535..2179)
                     /locus_tag="VLF19_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HSE93601.1"
                     /translation="MLVADVMREQLVTVTPRTTLPEAIRLMIDHRVRHLPVMDAGRLE
                     GIVSDRDVKRAMASPATSLAAHELNYLLDALAVEHFMTRAVVTVAPGLPVEDAARLML
                     SEQISALPVTEEGRLIGIVTETDVLRLFVRALGAGEPSSRLDVILDDRPSALAEVIRA
                     VEDAGAVVSSVMTLFKERVKEAVVRVATIDPGPAVAALQARGFQVRDGYRQAVR"
     gene            complement(2230..2775)
                     /locus_tag="VLF19_09875"
     CDS             complement(2230..2775)
                     /locus_tag="VLF19_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE93602.1"
                     /translation="MSTEILFATDLSDCSAPAGRVATEYARRLGARLHVLHVRPTAAA
                     RPEPALERLAAELGAASSVTAVETGPPVAERIVGYAARHAVELIVVGTHGRTGVSRVL
                     LGSVAEHVVRASTCPVLTVPCAWSGAPVAGPAPAAATPELRRCLVCREVTDDLTCAPC
                     RARVRGETLERKQREERAARL"
     gene            complement(2772..4088)
                     /locus_tag="VLF19_09880"
     CDS             complement(2772..4088)
                     /locus_tag="VLF19_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSE93603.1"
                     /translation="MNPTRVLVLGAAGRDFHDFNVAFRDDPAVEVVGFTAAQIPGISG
                     RRYPPALAGARYPDGIPIDAEEDLERLVRDRHVDQVVFAYSDVSHEHVMHLASRTLAA
                     GADFALLGPRRTMLRSRRPVVAVCAVRTGCGKGALCRRIVRIVRSWGRRVAVVRHPMP
                     YGDLEAARVQRFGALEDLDRAGVTLEEREEYEPHVEAGTVVFAGVDYAAVLARAEAEA
                     DVIVWDGGNNDLPFFAPTVHFCLVDPHRAGHESRYHPGEANVRAADVLVIVKEDTADP
                     EAVRAVRRAIRGLNPRAPVLDTRLPLHVDRPDALCGRRVLAVEDGPTTTHGEMAIGAA
                     AYAARRHGAVLVDPRPHAKGNLREVLAAHPSLGPVLPALGYGRAQLEELEATIDAVPC
                     DLVLLGTPIDLCRVITIRRPVMRVRYELDAEPGALEAALEPVGGAR"
     gene            complement(4107..4451)
                     /locus_tag="VLF19_09885"
     CDS             complement(4107..4451)
                     /locus_tag="VLF19_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSE93604.1"
                     /translation="MTLDIDGLTGDRALAARIRVRLGRELRRLTVTPVSAVAAFVDDD
                     GPKGGPARRCALTVRLPYRPALRAEESASTDRLAFDGAMANLERQLERYRERQRERGR
                     RPKKYYAAKRLL"
     gene            complement(4448..4903)
                     /locus_tag="VLF19_09890"
     CDS             complement(4448..4903)
                     /locus_tag="VLF19_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSE93605.1"
                     /translation="MKTSECMSRNVRTIEASASCREAVALMYRGKIRHLPVVDGTGRV
                     VGVVTDRDLRHRLFEPAVFREIGNVPVDSLLEATPVSAVMSTPAVTAGPDDELQDAAA
                     RMRMDGVGSLPVVEAGRVLGIITETDLLRRIVQADACCEEVGTIVVSYP"
     gene            complement(5034..5432)
                     /locus_tag="VLF19_09895"
     CDS             complement(5034..5432)
                     /locus_tag="VLF19_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93606.1"
                     /translation="MRNIGVPVLIVVLLGAPSTTTGTAGSAIRHSGIVLALEPELLVL
                     EEVGPWRVENGRTVVTRRTIKLTPATKFNTFIRVDVVGAFAGDFIEVALDAGDVTPGD
                     FVTAECRSVGGRLVAITVTVAESVTELSPK"
     gene            complement(5440..7053)
                     /gene="groL"
                     /locus_tag="VLF19_09900"
     CDS             complement(5440..7053)
                     /gene="groL"
                     /locus_tag="VLF19_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739228.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSE93607.1"
                     /translation="MPAKRVLFGSEARERILRGATALADAIRVTLGPRSKCVLIQRRF
                     GPPIVCNDGVTIAKEMELEDPEENLGAQMIRQAAERTGDAVGDGTSTATILAHAIYAD
                     GLRNVVAGASAVDLKRGLDRGARVAVEALRTQSRPVKSRLEKAQVATISAHNDTSIGD
                     LVADAMEKVGDEGVITVEEAKTTETQLEVVEGMQFDRGYLSPYFITDAEKMEAVLEDA
                     HLLLSDRKIGVMKDLLPLLEQIARAGAPLLVVAEDVEGEALATLVVNKLRGTLRCIAV
                     KAPGFGDRRKEMLEDLAVLTGGRVITEELGIKLENLDLSHLGRTQRVVVDRERTTIIG
                     GRGDKAAIEGRKHQLRQQIEKTTSDYDREKLQERLAKLSGGVAVVRVGAPSEAEMKSR
                     KEALEDAISATKAAVAEGIVPGGGLALLRAIDAVEREEAAAAGDERTGLQILRRALES
                     PTRRIAENSAVDGGVVVAEMRRGAGARGFDAARREYVDLLEAGIVDPTKVVRVALENA
                     VSAASLLLLTEATMTEVPEKKEAHAPPLE"
BASE COUNT         1052 a         2829 c         2615 g         1049 t
ORIGIN      
        1 gcgcacggcc cccggcctgc ccgaggaggt gcggcgcctc ttcgtgacgg cgaccgaggt
       61 gtcggtgcac ggccacctgc gcgtccaggc ggcgttccag cggcacgtgg acaacgccgt
      121 ctccaagacg atcaacctgc cagagaacac cgaccaggaa tccgtcgcgg aagcattccg
      181 cgagggctgg cggctgggcc tgaaaggtgt gacggtgtac cgctcggggt cgaagggcga
      241 ccaggtggtc acgctcggcg tcggcgagga cgcgacggcg cgcgagttct tcgccaagtg
      301 cgacccgggc gcctgccgcg tctgattaca tgggggcccc gacatgggcg cccagccccc
      361 cacctcggca cgccccggcg cagcgggggc gcgccttcta cccccgacgg cctcccgagc
      421 gtcagatcac gttactgcaa tgccgcgagg taggccgcga gagcttccac ctccgccccg
      481 gtcaattcga tcttcggcat gtgagcgctg gggcgctgga cctccgggtc gcggagccac
      541 cggagcaggt acggctgccc gtacttcgcg ccgacgtggg agaggtcggg gccgatcggc
      601 gtgccgaagg ctcccaccat gtggcagcca tagcagcctt gaacccggaa gaccctctgc
      661 ccctcggcga cgagcgcttt gctgtcctgc gcacacgcgg gcgccgccgc caggatcagc
      721 acgacgagca cgatcttcac gacgaacatg cgatatccct ccacttccat gcccagcaac
      781 acgggtgcca ctcgccggtg tcgaagaatc aacaccttgt gtcgggccgg gaggagcccc
      841 gtgaggcatc cgcgccccaa gtgcgggggg gccatcatga agggcgatga caagggtgcc
      901 gcgcgcctga cagaatggtg ccgccatgac ggggtcgcag accgagacgg cggggccgta
      961 tccgaaggag ctggagcgcg acgtgttgtt gaaggacggg gagcggctcc gcatccggcc
     1021 gatccgcagc gacgacgccc agcggctgat cgagctctac gaccgcttca gccgccacac
     1081 cgcctaccag cgcttcttca ccgtgatgcg gcggctgccg ccggactggg cgcgcatgct
     1141 cgcgaccgtc gactaccggc ggcggctcgc gctcgtcgcc gagcgcgaga cgccgggcgg
     1201 ccccgagctc gtcggcgtcg cgcgctacga gccgacggac cgcgacgaca cggccgaggt
     1261 cgcgttcgcg atccaggacg gatggcagaa tcgcggcctc ggcaccgccc tgttcgcgga
     1321 cctgctcgac gccgccgagg cgcgcggcat ccggcgcttc gtcgcctacg tcctggccga
     1381 caacatccgg atgctcgacc tgatcacgcg cttcggcgcc gtcgagcggc ggacgctcga
     1441 gcagggcgtc gtggagctgg tcttcacgcc gcgcggcgcc cccacggcgc gtgtccactg
     1501 atcctgccgg ccgtcggcac gcacgttcgc tcgctcagcg gaccgcctgc cggtagccgt
     1561 cgcgtacctg gaagccccgg gcctgcagcg cggccaccgc cggtccgggg tcgatggtcg
     1621 ccacccgcac cacggcctcc ttcacccgct ccttgaacag cgtcatcacg ctcgacacca
     1681 cggcccccgc gtcctccacc gcgcggatca cctcggcgag cgccgacggc cggtcgtcga
     1741 ggatcacgtc gaggcgactc gagggctcac ccgcgccgag cgcgcggacg aagagacgga
     1801 gcacgtccgt ctccgtgacg atgccgatca ggcggccctc ctcggtgacc ggcagcgcgc
     1861 tgatctgctc gctcagcatg agccgcgccg cgtcctccac gggcagaccc ggcgcgacgg
     1921 tgacgacggc ccgtgtcatg aaatgctcga cggccagcgc gtcgagcagg taattgagct
     1981 cgtgagcggc gaggctggtc gccggcgacg ccatcgcccg cttgacgtcg cgatccgaga
     2041 cgataccctc cagccggccg gcgtccatca ccggcaggtg acggacacga tggtcgatca
     2101 tgagccggat cgcctcgggc agggtcgtcc gtggcgtcac ggtgacgagt tgctcgcgca
     2161 tcacgtcggc gaccagcatc gccggtcctc cctctcgtca aggacttcgg tctcgctgca
     2221 acctccgcct cagaggcggg cggcgcgctc ctcgcgctgc ttgcgctcga gcgtttcgcc
     2281 gcggacacgg gcgcggcacg gcgcgcaggt caggtcgtcg gtgacctcgc ggcacaccag
     2341 gcaacggcgc agctcgggcg tggcggccgc cggcgccggc cccgccaccg gcgcgcccga
     2401 ccaagcgcac ggcacggtga gcaccggaca ggtcgaggcc cggacgacgt gctcggccac
     2461 gctgcccagg agcacgcggc tcacgccggt gcgtccgtgc gtgcccacga cgatgagctc
     2521 gacggcgtgg cgcgcggcgt agccgacgat cctctccgcg acggggggac ctgtctccac
     2581 cgccgtcacc gaggatgcgg ccccgagctc cgcggcgagc cgttccagcg ccggctccgg
     2641 cctggcggcg gccgtgggcc gcacgtgcag gacgtgcagc cgcgccccca gccgccgcgc
     2701 gtactcggtg gcgacgcgcc cggccggggc ggagcagtcg gacaggtccg tggcgaagag
     2761 aatctcggtg ctcatctcgc gcctcccacc ggctccagcg ccgcctcgag ggcgccgggc
     2821 tcggcgtcga gctcgtacct gacgcgcatg acggggcggc ggatcgtgat gacgcggcac
     2881 aggtcgatcg gcgtgccgag gagcacgagg tcgcacggca ccgcatcgat cgtcgcctcc
     2941 agctcctcga gctgcgcgcg cccgtagccg agcgccggca gcaccggacc gaggctcggg
     3001 tgcgcggcga gcacctcccg gaggttgccc ttggcgtgcg gccgcgggtc gacgaggacg
     3061 gcgccgtggc gccgggccgc gtacgccgcc gcgccgatcg ccatttcccc gtgggtggtc
     3121 gtcggcccgt cctcgacggc cagcacgcgg cgcccgcaga gggcgtccgg ccggtcgacg
     3181 tgcagcggca ggcgcgtgtc gaggaccggc gcgcgggggt tcagcccgcg gatcgcgcgc
     3241 cgcacggccc ggacggcctc gggatcggcc gtgtcctcct tgacgatcac gaggacgtcg
     3301 gcggccctca cgttcgcctc gcccgggtgg tagcgcgact cgtggccggc ccgatgggga
     3361 tcgaccagac agaagtggac ggtcggcgca aagaacggca gatcgttgtt gccgccgtcc
     3421 cagacgatga cgtcggcctc cgcctcggcg cgcgccagca ccgccgcgta atcgacgccc
     3481 gcgaacacga ccgtgccggc ttccacgtgg ggctcgtact cctcacgctc ctcgagcgtc
     3541 accccggccc ggtccaggtc ttcgagcgcg ccgaaccgct gcacccgcgc ggcttccagg
     3601 tccccgtagg gcatcgggtg ccggaccacg gccacccttc ggccccacga ccggacgatg
     3661 cgcacgatcc ggcggcagag cgcccccttc ccgcagccgg tcctcacggc acacacggcg
     3721 acgacgggcc ggcgcgagcg cagcatcgtg cggcgcgggc ccagcagcgc gaagtccgcg
     3781 cccgcggcca gcgtccggct ggcgaggtgc atgacgtgct cgtggctcac gtcgctgtag
     3841 gcgaagacca cctggtccac gtggcggtcg cggaccagac gctcgagatc ctcctcggcg
     3901 tcgatgggga tgccgtcggg gtaccgcgct ccggcgagcg ccggcggata gcggcgtccg
     3961 ctgatccccg gaatctgcgc ggccgtgaat ccgaccacct ccaccgccgg atcgtcgcgg
     4021 aaggccacgt tgaagtcgtg aaagtcgcgc cccgcggcgc cgaggacgag gacgcgtgtc
     4081 ggattcacgg cgcccgggac acctggctac agcagccgct tcgcggcgta gtacttcttc
     4141 ggtcggcggc cgcgctcccg ctgccgctcg cgataccgct cgagctgccg ctcaaggttc
     4201 gccatggcgc cgtcgaaggc caggcggtcg gtcgacgcgc tctcctcggc gcggagcgcc
     4261 ggccgatagg gaagccggac ggtcagcgcg caccgacgcg cgggcccccc cttcggtccg
     4321 tcgtcatcga cgaatgccgc gacggcggac acgggcgtca ccgtcagacg gcgaagctcg
     4381 cgccccagac gcacccggat ccgcgcggcc agggcacggt caccggtcag gccgtcgatg
     4441 tcgagggtca tgggtaggac accacgatcg tgccgacttc ctcgcagcac gcgtcggcct
     4501 gcacgatccg gcgaaggaga tcggtctcgg tgatgatgcc gagcacgcgc ccggcctcga
     4561 cgacgggcag cgagcccacc ccgtccattc gcatccgcgc cgccgcgtcc tgcagctcgt
     4621 cgtccggacc ggccgtcacg gcgggcgtcg acatcacggc gctcaccggc gtcgcctcga
     4681 ggagcgagtc caccggcacg ttgccgatct cgcggaagac ggccggttcg aagagccggt
     4741 ggcgcaggtc gcggtcggtg acgactccga cgacgcggcc cgtcccatcc accactggca
     4801 ggtggcggat ctttccccgg tacatcagcg cgaccgcctc ccggcaggac gcgctcgcct
     4861 cgatcgtccg cacgttccgg ctcatgcact cgctggtctt catgccgtcg tacggagcaa
     4921 cgccggtgcc acgggtggca cgcgacaatc tgcggtgtta cccgacacgc gtggcggcgc
     4981 cagtggggcg cgcgcgccgc gttgccccat cggcgcctca ggcgaggaca ctctcatttc
     5041 gggctcagct ccgtcacgct ctcggccacg gtcaccgtga tcgccacgag ccggccgccg
     5101 acggagcggc actcggccgt cacgaagtct ccgggggtca cgtcgccggc gtcgagggcg
     5161 acctcgatga agtcaccggc gaaggccccg acgacgtcga cgcggatgaa ggtgttgaac
     5221 ttcgtcgcgg gtgtcagctt gatcgtccgg cgcgtcacga cggtccggcc attctcgacg
     5281 cgccaggggc cgacctcctc gagcacgagg agctcgggct ccagcgcgag gacgatgccg
     5341 gagtgccgga tggccgaccc ggcggtgccg gtggtcgtcg agggtgcacc cagcaggacc
     5401 acgatgagga cgggcacacc gatgttcctc atgggagcct cactcgagcg gaggcgcatg
     5461 cgcttccttc ttctcgggca cctccgtcat ggtggcctcc gtgagcagca gcaggctcgc
     5521 cgccgacacg gcgttctcga gggccacccg caccaccttc gtcgggtcca cgatgcccgc
     5581 ctccaggaga tcgacgtact ccctccgcgc ggcgtcgaag ccccgcgcgc cggcaccgcg
     5641 gcgcatctcg gccaccacca cgccgccgtc cacggcggag ttctcggcga tccggcgcgt
     5701 gggcgactcg agcgcgcggc ggaggatctg caggcccgta cgctcgtcgc ccgcggccgc
     5761 cgcctcctcg cgctcgaccg cgtcgatggc ccgcaggagc gcgaggccgc cgcccggcac
     5821 gatgccctcg gccaccgccg ccttggtggc gctgatcgcg tcctcgagcg cctccttgcg
     5881 gctcttcatc tccgcctcgg acggtgcccc gacccggacc acggcgacac cgccggagag
     5941 cttggccagg cgctcctgga gcttctcgcg gtcgtagtcg ctcgtcgtct tctcgatctg
     6001 ctgccggagc tggtgcttgc gtccctcgat ggccgccttg tcgccccggc cgccgatgat
     6061 cgtcgtgcgc tcgcggtcga cgaccacccg ctgcgtccgg cccagatggc tcaggtcgag
     6121 gttctccagc ttgatgccga gctcctcggt gatgacgcgc ccgccggtga ggaccgcgag
     6181 gtcctcgagc atttccttgc gccggtcccc gaaccccggc gccttgacgg cgatgcaccg
     6241 cagcgtgccg cgcagcttgt tgaccaccag cgtggcgagc gcctcgccct ccacgtcctc
     6301 ggcgacgacg aggaggggcg cgccggcccg ggcgatctgc tcgagcagcg gcagcaggtc
     6361 cttcatgacg ccgatcttgc ggtcgctgag caggagatgc gcgtcctcca gcacggcctc
     6421 catcttctcg gcgtcggtga tgaagtacgg cgacaggtac ccgcggtcga actgcatgcc
     6481 ctcgaccacc tccagctggg tctccgtcgt cttggcctcc tcgacggtga tgaccccctc
     6541 gtcgccgacc ttctccatgg cgtccgccac gaggtcgccg atcgaggtgt cgttgtgcgc
     6601 ggagatcgtg gccacctgcg ccttctcgag ccggctcttg accgggcgtg actgcgtccg
     6661 gagcgcctcc accgcgacgc gcgcgccgcg gtccaggccg cgcttgaggt ccacggcgct
     6721 ggcgccggcg accacgttgc gcagcccgtc ggcgtagatc gcgtgcgcga ggatcgtcgc
     6781 ggtgctggta ccgtcgccca cggcgtcgcc ggtccgctcc gccgcctggc ggatcatctg
     6841 ggccccgagg ttctcctccg ggtcctcgag ctccatctcc ttggcgatgg tcacgccgtc
     6901 gttgcacacg atcggcgggc cgaagcgccg ctggatcagc acacacttcg accgaggtcc
     6961 cagcgtcacc cggatggcgt cggcaagggc cgtcgcgccg cgcaggatcc gctcacgcgc
     7021 ctccgacccg aacagcacac gcttggccgg catccctcgc tcctttcacc gccacgtgcg
     7081 aagcagccag ataccgagca cgatcaccgc gacgaccgcc acggcgatga gccactgatc
     7141 acgaatcgcc tcgaccattg cggactcccg ccgtgcacgg tgcaacgagg ctgccacatc
     7201 ggacgcctgc aaattccagt gcttccgcgg cgggcaccgg cgacggtgtc ccggcccaac
     7261 cccatctggg gcgagcccgc cacggcggcg ctcactcgcc gaagagccgg cgcgggcgga
     7321 cccgcggtcc acaggagagc gccgccagta ctccctgccc atcagcacga tggggaagtc
     7381 gtgcaccttg cgggcctgca ccagcgtggc gatctcgaag aggcccgcgc cagatcgaat
     7441 ccgcgcggct gcgggccctc gagaaagcgg atggcgtccc gttcgtcgcg ctcggcgacg
     7501 gcgacgggca gggcggtcgt caccacagat acgtgtcggg gtcga
//
LOCUS       DATIAZ010000280         6297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1790264, whole genome shotgun sequence.
ACCESSION   DATIAZ010000280 DATIAZ010000000
VERSION     DATIAZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790264"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VLF19_09905"
     CDS             <1..319
                     /locus_tag="VLF19_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSE93608.1"
                     /translation="WVGREGERLLVCTSEGSLKGRNTRRDPRVALSIVDFHDPYEEVQ
                     LRGRVVERRPDPELKIMDPVSHKYIGRPFPMRGAEGRVALVIEIDKARYTKLPFSHTP
                     PRQ"
     gene            complement(345..1544)
                     /locus_tag="VLF19_09910"
     CDS             complement(345..1544)
                     /locus_tag="VLF19_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93609.1"
                     /translation="MRWILRLLLMSALMLALPLTASDQAVRPPLKIGLLLPYTGVIAI
                     NGQETSKGVEFYLSKVGSKAGGRAIQLIKEDDEAKPDVGLTKARKLVERDKVDVLIGP
                     VHSGVALAIRDYVNEQGIPLIIPVAFTRDITAPGKASPWIFRVVETTDQGNYPMGTWI
                     VKNTPHRKIVVMASDFVAGRHSVEAFVAAVRAAGGQIVKEIYAPLNTADFAPFIAQVS
                     GLGADALYAWFAGADSIRFVKAYREYGLADKLPLFAYNTLVDDVLLPTLGDAALGIVS
                     VGHYSAALDTPANRTFVSEYEARYRGWPTRYVELGYVAGQLVGAAVEALRGEVGDRAR
                     FRDAIRSAVTTIKPPRGPIQFDRYQQVITDVYVMKVERQGNRLVNAIVDRIPRISQED
                     TWKWWNK"
     gene            complement(1567..1800)
                     /locus_tag="VLF19_09915"
     CDS             complement(1567..1800)
                     /locus_tag="VLF19_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HSE93610.1"
                     /translation="MNRGTGVGGRSRVGRSTARERVQLFHLAWDLACSSFGSRQVLYE
                     RFFQGDWMRNATLLLGSYDVEPLVQQVRQFMGK"
     gene            complement(2066..4048)
                     /locus_tag="VLF19_09920"
     CDS             complement(2066..4048)
                     /locus_tag="VLF19_09920"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSE93611.1"
                     /translation="MALVIAACAPLAARRELPPPWAPLEAALPPIPFRDGLLALTLVH
                     PPEGGEITVRDRTFVFGTAGTGRARVAINGAPVDVAPNGAFLAFLPVPADGVYRAEAR
                     VGEEHAEVIRRVRVPETPAARAHGEAFIDEPSVFPRGAWAAMPGEPIEVGFRGTAGGE
                     AVLVLGDGSRIPLVEQAPVVEVPWGRRSFGAVPPSPEPAIPGLVHYRGMLVARPLLAS
                     EPDVPRPALAPLPTSTRSGAAVELKAGGHVVRVPLRLNLALLDPEQPAVGVVHEPDDA
                     RRSTGVDAAAGPASTYHYFWDNGTRLTLTGEQAGEYRVRLTPDLHAWVAADRVRLLPP
                     GTPPPRGRVGTVRLTPRPEAVAVRIEVDRPLPYRVESRGSSVAITIYGGFADTRWLMS
                     GGLDRHVIGARWSQPADGLWVLTLDLAQPHWGHSASWDPNGDLVVRVRRPPPLDRRRP
                     LRGLVVAVDPGHPPGGATGPTGLGEAEVNLAVALRLARMLERAGARVILTRTDDTALG
                     LDERARIAEVAGAHLFVSLHQNAFPDGVDPFVDSGTSTFYFHPHAAALARALQAELIQ
                     EFRLRDLGVGRASLAVLRRVTWMPAALTETMFMMMPPQEAALRDPDVQERIARAHLRG
                     LERFLREQHRASRSSAAATAPPALTPQAAVSDARAR"
     gene            complement(4119..5546)
                     /locus_tag="VLF19_09925"
     CDS             complement(4119..5546)
                     /locus_tag="VLF19_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HSE93612.1"
                     /translation="MARVLFPAIRWDPGQGFAPAARSIDVALELGVGGFILFGGPAAA
                     VRELTGDLRARSAAPLVIGADLERGAGQQFAGAVSLPPAAAFGAIGDLGATRRAGELT
                     GREARALGVDWIYAPVADLDAEPSNPIVGTRAYGADPAVVSAHVAAWIDGCRSAGALA
                     CAKHFPGHGRTVTDSHLGLPVVEASRAALEADLRPFRAALEHGVDAVMTAHVAYPAVD
                     PGGGPATLSAAIVTGLLRGELGFRGLVVTDALVMAGVLGPVADEAQAAVAALAAGCDV
                     LLCPADPERVAAALESARGRALPEARIAEAARRVEDAACRVASPRPGEWDRGEDRDWA
                     CDIAARALFVVRGAPALAGRRIDLVTVDDDVGGPFPPGPRASFPATLTASGIEVREAA
                     APAGPAVVALYADVRGWKGRPHVSRAARERLEQALAVRPGATVVLFGHPRLAAQVPGD
                     AVLAAWSGDPVMQEAAARWLAGHAR"
     gene            5734..>6297
                     /locus_tag="VLF19_09930"
     CDS             5734..>6297
                     /locus_tag="VLF19_09930"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSE93613.1"
                     /translation="MSAGFRDFLRQLEDAGELLRLPKRVDPRHVSALMAQASQAAFFE
                     TLDGYADWRAAGALVSTRKRLAIAMGCREDAIATRFEQGIQRPIEPRLVDRAPCQEVV
                     WTGDDVDLTRIPIPMMHVKDGGPYISATVVVSRDPEYGRNVGSYRLMYRTPRETGIDL
                     VSSSDMRVYYQRALDQGRPLEIAVAIGT"
BASE COUNT          935 a         2286 c         2255 g          821 t
ORIGIN      
        1 ctgggtcggg cgcgaggggg agcggctcct ggtgtgcacg agcgaggggt cgctcaaggg
       61 gcgcaacacg cgccgcgatc cccgagtcgc cctgtccatc gtggacttcc acgatcccta
      121 cgaggaggtg cagctccgcg gccgcgtggt cgagcgccgt cccgaccccg agctcaagat
      181 catggatccc gtgtcgcaca agtacatcgg aagacccttc ccgatgcggg gcgccgaggg
      241 ccgcgtggcg ctcgtgatcg agatcgacaa ggcgcgctat accaagctgc cgttctccca
      301 cacgccaccc cggcagtagc gcgccgagga cccgggcgcc gggctcactt gttccaccac
      361 ttccaggtat cctcctggga gatccgggga atccggtcga cgatggcgtt gacgaggcgg
      421 ttgccctgcc gctccacctt catgacgtac acgtccgtga tcacctgctg gtagcggtcg
      481 aactggatcg gcccacgcgg gggcttgatc gtcgtcaccg cgctccggat cgcatcccgg
      541 aacctggccc ggtcaccgac ctcgccccgg agcgcctcca cggctgcgcc cacgagctgt
      601 cccgccacgt agcccagctc gacgtagcgg gtcggccatc cgcggtaccg ggcctcgtac
      661 tcggacacga aggtccggtt cgccggcgtg tccagggcgg cgctgtagtg tccgacgctc
      721 acgatcccga gcgcggcgtc gccgagcgtc ggcagcagca catcgtcgac gagcgtgttg
      781 tacgcgaaga gcggcagctt gtcggcgagc ccgtattccc ggtaggcctt cacgaatcga
      841 atcgagtccg ccccggcgaa ccaggcgtag agggcatccg cgccgagccc gctcacctgg
      901 gcgatgaacg gcgcgaagtc ggccgtgttc aggggcgcgt agatctcctt cacgatctgc
      961 ccacccgcgg cgcggacggc cgccacgaag gcctccacgg aatgccgtcc ggccacgaag
     1021 tcggaggcca taacgacgat tttccggtgc ggggtgttct tcacgatcca ggtccccatt
     1081 gggtagttgc cctggtcggt ggtctcgacg acgcggaaga tccaggggct cgccttgcca
     1141 ggcgcggtga tgtcgcgggt gaacgcgacc gggatgatca gcgggatccc ctgctcgttc
     1201 acgtagtcgc ggatggcgag ggccacgccg ctgtggacgg ggccgatgag gacgtcaacc
     1261 ttgtcgcgct cgacgagctt gcgcgccttg gtcagcccca cgtcgggctt cgcctcgtcg
     1321 tcctccttga tgagctggat cgcgcgcccc ccggccttgc ttccgacctt cgagaggtag
     1381 aactccacgc ccttgctcgt ctcctggccg ttgatggcga tcacgccggt atagggcagg
     1441 agcaacccga tcttgagcgg tggtctcacg gcctgatcgg acgcggtcag aggcagggcg
     1501 agcatgaggg ctgacatcaa gagcagtcgt aggatccatc gcatggtcat gcctcctgcg
     1561 gcggcttcac ttccccatga actgccgtac ttgctgcacc agcggctcga cgtcgtagct
     1621 gcccaggagg agggtcgcgt tccgcatcca gtcaccctgg aagaagcgct cgtagagcac
     1681 ctggcgcgag ccgaaggagc tgcaggccag gtcccaggcc aggtggaaca attgcacccg
     1741 ctccctggca gtggacctcc cgacgcggct ccgcccaccc actcccgtgc cgcggttcac
     1801 cgcgtctcac atgcacgtca cgacgacctt cacccgctgg tgcggccgcc tggcggcagc
     1861 tccatcgtcc tgctcccccg cggcaagggt caaagagccc aggtcggcgc cgtcttcgcc
     1921 gctggcgcga ttgtagcgcg ctgcgctcgc tctcacgcga gagggccccg acatcggtgc
     1981 gcgcgtcaag gagttcacga cacgacgtgc tgcgcgtgca ttcggcccac gtccggcaag
     2041 agagggtcac gtggcgccgc gcgagctatc gggcgcgggc gtcgctcacc gccgcttgcg
     2101 gcgtcagcgc gggcggtgcg gtcgccgccg cggacgagcg cgaggcgcga tgctgctcgc
     2161 ggaggaagcg ctcgaggccc cggaggtgcg cgcgggcgat ccgttcctgg acatcggggt
     2221 cgcgaagggc cgcttcctgg ggcggcatca tcatgaacat ggtctcggtg agcgccgcgg
     2281 gcatccacgt gacccggcgc agcaccgcga ggctcgctcg tcccacaccg agatcgcgca
     2341 gccggaactc ctggatcagc tccgcctgaa gcgcgcgggc cagggcggcc gcgtggggat
     2401 ggaagtagaa cgtggaggtg ccgctgtcga cgaaggggtc gacgccgtcc gggaaggcgt
     2461 tctggtgcag cgacacgaag aggtgggcgc ctgccacctc ggcgatccgg gcccgctcgt
     2521 ccagcccgag cgcggtgtcg tcggtccgcg tcaagatcac ccgcgcaccc gcgcgctcga
     2581 gcatccgcgc aagccggagg gcgacggcga ggttgacctc ggcctcgccg agccccgtgg
     2641 gacccgtggc gccgcccggc ggatggcccg ggtcgacggc cacgacgagg ccgcgaagcg
     2701 ggcggcgccg gtcgagcggc ggaggccgcc ggacgcggac cacgaggtcg ccgttcgggt
     2761 cccacgacgc tgagtggccc cagtgcggct gggccagatc cagcgtcaga acccagagcc
     2821 cgtcggcagg ctggctccag cgggcgccga tgacgtggcg atcgagaccg ccggacatga
     2881 gccagcgggt atccgcgaac ccgccgtaga tggtgatggc gaccgagctc ccgcgcgact
     2941 cgacacgata gggtagcgga cggtcgacct cgatccgcac cgcgacggcc tcgggccggg
     3001 gcgtcagcct gacggtgccg acgcgcccgc gcggcggcgg cgtgccgggc ggcagcaagc
     3061 gcacccgatc ggccgccacc caggcgtgga ggtcgggcgt caggcgtacg cggtactcac
     3121 cggcctgctc gccggtcagc gtgaggcgcg tcccgttgtc ccagaaatag tgataggtgg
     3181 atgctggccc cgcggcggcg tccacgcccg tcgatcggcg agcatcatcg ggctcgtgga
     3241 ccacgccgac cgccggctgt tcggggtcga ggagagcgag gttgagccgg agcgggacac
     3301 ggacgacgtg tccgccggcc ttcagctcca cggcggcacc cgatcgcgtc gacgtgggga
     3361 gtggcgccag cgccgggcgc ggcacgtcgg gctcggacgc gaggagcggt cgggcgacaa
     3421 gcatgccgcg atagtgcacc aggccaggga tggccggctc cggcgagggc ggcaccgcgc
     3481 cgaaggagcg gcggccccac ggcacctcga ccaccggggc ctgctcgacg agcgggatgc
     3541 gcgagccatc cccgaggacg aggaccgcct cgccgccggc ggtgccccga aatcccacct
     3601 cgatgggctc gcccggcatc gcggcccacg cgccgcgggg aaacacgctc ggctcgtcga
     3661 tgaacgcctc gccgtgcgcc cgcgcggccg gcgtctccgg cacccggaca cggcggatca
     3721 cctcggcgtg ctcctctccc accctcgcct ccgcccggta gacgccatcg gcaggcaccg
     3781 gcaggaaggc caggaaggcg ccgttgggcg cgacgtcgac gggagcgccg ttgatggcga
     3841 cccgggcgcg acccgtgccg gccgtcccga acacgaacgt tcggtcacgc acggtgatct
     3901 cgcccccctc ggggggatgc acgagcgtca gcgcgagcaa cccgtcgcga aacgggatgg
     3961 gcggcagggc cgcttcgagc ggcgcccacg gcggcggcag ctcccgccgt gctgcgaggg
     4021 gcgcgcaggc ggcgatgacg agcgccatga gcgtgatggg cgtcgccggc cgcaggtcta
     4081 gctcctcgga accggttcga ccgcgaagtc gaccgctctc atcgagcgtg gcccgcgagc
     4141 cagcgggccg ccgcctcctg catgacggga tcgccgctcc acgccgccag cacggcgtca
     4201 cccggcacct gggcggccag acgcggatgg ccgaacagca cgacggtggc ccccggcctc
     4261 accgcgagcg cctgctcgag gcgctcccgg gcggctctcg agacgtgcgg ccggcccttc
     4321 cagccgcgga cgtccgcgta gagcgcgacc accgccggac cggccggcgc cgcggcctcg
     4381 cgcacctcga tcccggacgc cgtcagcgtg gctgggaacg acgcgcgcgg tccgggagga
     4441 aacggaccgc cgacatcgtc gtcgacggtg accaggtcga tgcggcgacc ggccagcgcc
     4501 ggcgctcccc gcacgacgaa cagcgcccga gccgcgatgt cgcacgccca gtcgcggtcc
     4561 tccccccgat cccattcgcc cgggcgtggc gaagcgactc gacacgcggc gtcctcgaca
     4621 cgtcgggccg cctcggcgat ccgggcctcg ggcagtgcgc ggccgcgggc cgactccagc
     4681 gccgcggcca cacgctccgg atcggcggga cagagaagca cgtcgcatcc ggcggccagc
     4741 gcggccaccg ccgcctgggc ctcgtcggcg acgggaccga ggacgccagc catgacgagc
     4801 gcgtccgtca cgacaaggcc gcgaaagccg agctctcctc gcaggaggcc ggtgacgatg
     4861 gcggctgaaa gcgtcgcggg tcccccgccc ggatcgaccg ccgggtaggc gacatgcgcc
     4921 gtcatcacgg catccacacc gtgttccagg gcggcgcgga agggacgcag atccgcctcg
     4981 agggccgcgc gggaggcctc gaccacgggc aggcccaggt gcgagtccgt cacggtgcga
     5041 ccgtgcccgg ggaagtgctt cgcgcaggcg agcgccccgg cgctccgaca cccgtcgatc
     5101 caggcggcga catgcgccga cacgacggcg gggtcggcgc cgtaggcgcg ggtccccacg
     5161 atcgggttcg acggctcggc gtcgaggtcg gccaccggcg cgtagatcca gtcgacgccg
     5221 agcgcccgcg cctccctccc cgtgagctcg ccggcacggc gcgtggcgcc gaggtcgccg
     5281 atcgctccaa aggcggccgc cggcgggagc gagaccgcgc cggcgaactg ctggcccgcg
     5341 ccccgctcca gatcagcgcc gatgaccagc ggcgccgcgc tcctggcgcg gaggtcgccc
     5401 gtcagctccc ggactgccgc ggccggtcct ccgaagagga tgaatccgcc cacgcccagc
     5461 tcgagcgcca cgtcgatgct ccgcgcggcc ggcgcgaagc cctgccccgg atcccaccga
     5521 atggcgggaa acaggacccg agccacgcgc gtcctcgcct tggggcgccg gccttgaaca
     5581 ccgtgagcct tcgacaccga gacgagccta cccgaagacc aggtcgacgc atctgaaaga
     5641 tcatcgagga cttcggtcac gccgtggccc cggccgtccg gtccggcggg gtacgcgatg
     5701 gcgacggcgt cgtcgatggt agactttcca ccgatgagcg ccggcttccg ggactttctc
     5761 aggcagctcg aggacgccgg cgagctgctg cggctcccga agcgcgtcga tccgcggcac
     5821 gtgtctgcgc tcatggcgca ggcgtcgcag gccgcgttct tcgagacgct cgacggctat
     5881 gcggactggc gcgccgccgg cgccctcgtc tccacgagga agcgactcgc gatcgccatg
     5941 gggtgccggg aagacgccat cgccacgcgg ttcgagcagg gcatccagcg gccgatcgag
     6001 ccacgactcg tcgaccgcgc gccgtgccag gaggtcgtgt ggaccggcga tgacgtggac
     6061 ctgacacgca tcccgatccc gatgatgcac gtcaaggacg gcggacccta catctcggcc
     6121 acggtcgtgg tgtccaggga ccccgagtac ggccgcaacg tcgggtcgta ccgcctgatg
     6181 taccgcacgc cgcgcgagac aggcatcgac ctcgtctcgt cgtccgacat gcgcgtgtac
     6241 taccagcgcg cgctcgatca ggggcggccg ctggagatcg ccgtcgccat cggcacg
//
LOCUS       DATIAZ010000281         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1659827, whole genome shotgun sequence.
ACCESSION   DATIAZ010000281 DATIAZ010000000
VERSION     DATIAZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659827"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VLF19_09935"
     CDS             complement(<1..993)
                     /locus_tag="VLF19_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93614.1"
                     /translation="MAVTVFDAFMRTAQAAPDNVFLCAPPAAGRAYHPGGVEFTYAQT
                     KGAALALMERYASAGYGHGHRVALLLENRPEFFFHYLALNALGCGIVPINPDYRHDEM
                     LYQMEHSEADLAVVIGSRAADVEAVARDRARVKALPVAAAEDLPAVLPEPGPPPRPGS
                     PGLGTETSLLYTSGTTGRPKGCVLTNLYYLNAGAWYRDLGGRIAIEPGRERFLNPLPL
                     FHMNAQAVTATCAILTASSLAQPERFSPGRWWKDVVASRATIIHYLGVMPPLLLNQAP
                     VPEERQHRVKFGLGAGVEPELHARFEERFGFPLVEVWGMTETGRIYADCREPRQI"
     gene            1231..>2224
                     /locus_tag="VLF19_09940"
     CDS             1231..>2224
                     /locus_tag="VLF19_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE93615.1"
                     /translation="MSAAGRDVTPLDELRLLVVVDNETDTLSSVAEGIPQVPEALRLA
                     SRLPAVRHHDGHPCKVVFDKLCCAAHGLSVLVTGRRGAEERTMLFDVGPYADVWQSNA
                     ERLGIDLAAIETIFLSHWHFDHSGALPEVVAAVVAARKAAGHAAPVVVDLHPDRPDQR
                     GTLAPNGTMILLPEEPRFEAVEAAGGRVARHAEAHVLCEGFFLGSGRIERVTEYEAGL
                     VGHHSFRGGRGEADPLILDERFVAAHVRGRGVTLLSACSHAGVVNAGLGAQSHFAGLP
                     IDVVLGGYHLSGKAMEARIEATVRDLESRVRPRLVAPGHCTGWRAKAALVRAFAP"
BASE COUNT          301 a          776 c          835 g          312 t
ORIGIN      
        1 gatctggcgc ggctcccggc agtccgcgta gatgcggccg gtctcggtca tgccccacac
       61 ctccacgagc gggaagccga agcgctcctc gaaccgcgca tgcagctcgg gctccacgcc
      121 ggcgcccagg ccgaacttca cgcggtgctg acgctcttcc ggcaccggcg cctggttgag
      181 cagcagcggc ggcatcacgc cgaggtagtg gatgatcgtc gctctcgacg ccacgacgtc
      241 cttccaccac cggcccgggc tgaaccgctc gggctgggcc agagagctcg cggtgaggat
      301 ggcgcaggtg gccgtgaccg cctgggcgtt catgtggaag agcggcaggg gattgaggaa
      361 gcgctcgcgc ccgggctcga tcgcgatgcg gccgcccaga tcgcggtacc aggcgcccgc
      421 gttgaggtag tagaggttgg tcaggacgca gcccttgggc cgccccgtgg tgcccgaggt
      481 gtagagcagg ctcgtctcgg tgccgagccc gggtgagccg ggacgcggcg gtggtccggg
      541 ctcgggcagg accgcaggca gatcttcggc agccgcgacg ggcagcgcct tgacgcgcgc
      601 gcggtcgcgg gccacggcct cgacgtccgc cgcgcggctc ccgatgacca cggccaggtc
      661 cgcctcggag tgctccatct ggtagagcat ctcgtcgtgg cggtagtcgg gattgatggg
      721 cacgatgccg cagcccagcg cgttgagcgc caggtagtgg aagaagaatt ccgggcggtt
      781 ctcgagcaac agcgccacgc ggtgaccgtg gccgtagccc gcgctcgcgt accgctccat
      841 cagtgcgagc gcggcgccct tcgtctgcgc gtaggtgaac tcgacgccac cggggtgata
      901 ggcgcgtccg gcggcggggg gagcgcacag gaacacgttg tcgggcgccg cctgtgccgt
      961 ccgcatgaac gcgtcgaaca cggtgacggc catggcccgg acgagtaccg cgcccggcgg
     1021 ggatgtgtca aggcgggcac gccgcgacgg cttgcgaatg gccggcactc gccggagaat
     1081 cgtcggcgcc ccgggagacc cgcgaggcgg gcccgatgag tcggggcgat cgacccgtgc
     1141 ctggaggacc gaacgccgca ccacttgcgc ggagctgtcg cccgcgaacg gcggcttccc
     1201 gcgtccatcc ggccacgagg aggactggac atgagcgccg ctggccgcga cgtgacaccg
     1261 ctcgacgagc tgcggctgct cgtcgtcgtg gacaacgaga ccgacacgct gtcgagcgtg
     1321 gccgagggca ttccgcaggt gcccgaggcg ctgcgcctcg cctcgcggct gcccgccgtc
     1381 cgccatcacg acggtcaccc gtgcaaggtc gtcttcgaca agctctgctg cgcggcccac
     1441 ggcctctcgg tgctcgtgac cggccgccgc ggcgccgagg agcgcacgat gctcttcgac
     1501 gtcggcccgt acgccgacgt ctggcagagc aatgccgagc ggctcgggat cgatctggcc
     1561 gcgatcgaga cgatctttct ctcgcactgg catttcgatc acagcggcgc gttgcccgag
     1621 gtggtggcgg cggtggtggc ggcgcggaag gccgcgggcc acgcggctcc ggtcgtggtg
     1681 gacctgcacc ccgaccgccc ggatcagcgc ggcacgctgg ccccgaacgg cacgatgatc
     1741 ctgctgccgg aggagccgcg cttcgaggcc gtagaggccg ccggcggccg ggtggcgcgg
     1801 cacgccgagg cccacgtgct ctgcgagggc ttcttcctcg gaagcggccg gatcgagcgc
     1861 gtcaccgaat acgaggcggg gctggtgggc catcactcgt tccgtggcgg ccggggcgag
     1921 gccgatccac tcatcctcga cgagcgcttc gtcgccgccc acgtccgcgg ccggggcgtc
     1981 acgctgctgt cggcgtgctc tcacgcgggc gtcgtcaacg ccggcctcgg cgcacagtcg
     2041 cacttcgcgg gcctgccgat cgacgtcgtg ctgggcggct atcacctgag cggcaaggcg
     2101 atggaggcgc gcatcgaggc gaccgtgcgc gatctcgagt cgcgggtccg gccacggctc
     2161 gtcgcgcccg ggcactgtac ggggtggcgg gccaaggccg ccctcgtccg ggcgttcgcc
     2221 cccg
//
LOCUS       DATIAZ010000282         5606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1791005, whole genome shotgun sequence.
ACCESSION   DATIAZ010000282 DATIAZ010000000
VERSION     DATIAZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791005"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..>5606
                     /locus_tag="VLF19_09945"
     CDS             110..>5606
                     /locus_tag="VLF19_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSE93616.1"
                     /translation="MATTAGARRFATLARLGQAVTASLDLGDVLDTVARAATDLLRGS
                     HTAIWVAEDERLVRRASAGGRESCGESCLREIAVGEGLIGGVAATRMPMAVADVSGEN
                     DSTGWVQQHQYVSFLAIPLLVRDRLVGVLSLGAWHRHVFSLHEMEVLGAFGDQAAIAV
                     GNAGLYATLIARVRRLHALTRMTRLISSSLDPDAVLQEIADAAASFGEARVASIWSAD
                     DVARALTLVASSREAMRDFPTPTMDYDAGILGWVATRREAAQVSDVFADTRFVVLDWW
                     RARGLSSFLGIPVVLDDELVAVLALHGDRPLAPTASEERDLLESFAAQAAVAIRNARL
                     FTDSERRRRAAEALQRIGRDLAHSLDPEEVGARVVASVLGLIGAKRASLYRLEDDSGD
                     LILLATAGEVKPANDVLPHGVGTAGLAAVSRRPVACSDVLADDRLTWTAAVRAAIEPT
                     RYRAVLSVPLLVRSRLFGVLAIADEAGRAFTIDEVSLAQAFADQAALALDNARLLHES
                     RTRQARLETLLEVSRELSGIQLLDRVLNRIAEACGRVLDSGSVGFRIVEDDELVVRGV
                     WGDAGAVMAKPRLKIGESLSGRVVATGQPLIVQQLADAPDLIPEHREQARLHGLRAFL
                     GVPVNVGERVAGVLTIRSLDDRAFGDAELATATAFAAQAAVALQNASLYGELQAQLRR
                     TEALLNVTRAVSTTLDLDEAMRRVARETARLLGADMVGAYLADADGKSLWPAAGYRVP
                     AELRDAFRRHPIPLAGHAFVEDAWHRRTTVWSSDVEREPGIDRETIARFPHRALLFSP
                     MVAQGRSIGGLFVLWWKDHRTPSAEEIRLLEGLADQAALSFETTRLYEQSERGRRAAE
                     ALLEVGQALASTLEPAEIFTLVTRRTAQALGAERCSLYELRHGRLVPLMSQYADGRTD
                     KTLWALFKTLPRHRPDDVPVYAEAVRTRRPVVVADATAPGAPVPPEWVDTFAIRSLLV
                     VPLFHQDKLLGMLNFDTTMPAPWRREQIDLAMTIANQTALAIENARLFAAQREEAQVS
                     SALLRLARSLEPLQDLNAVLEVAVARAPELLGLTRCSVLVVDPSTGELVPTVASGLAE
                     EHRPAFQALRGVGAVPAFAEAIRSHEVVAVPDATEDTWIPRAVARELDVQAMLIIPLV
                     SGGRLMGALIVDTPGRAAAFDDKQLAVARGIASHLAGAIDRAQLHAESERRRREAEIF
                     AELARTINASLDLPTVMQHVAEWARELCRSDGAAAALREGDVMTLRYWVGRDNVGPLT
                     VEPGKGAGGLVMTTGRPFRTADYASDPRITKDYADIARGLGAAAQLVVPIHIADQVEG
                     LLYAGNRAPRPFTERDEAILVRLAEHAAIALKNSRLFAREQESQRFLQSTLDALSAHV
                     AVLDETGTIIAVNGAWRRFADAHRLGDRPYGLGDNYLAVCEAARGEGAAEAAAVAAAI
                     RTVLRGERAEFHLEYDCHGPTDRRWFVVRITRFTGPGLVRVVVAHENISERRLAEEAL
                     QRNEEHVRQVQKMEAVGRLAGGIAHDFNNLLTVITGRTQLALRRLREDDPMRHDLGLI
                     RTTAERAATLTRQLLAFSRKQLLQPRVVEVEAVVRGLVPMLQRLLGEDVELIIPPVAG
                     PARVKADPGQLEQVIVNLAVNARDAMPQGGRLTVRIGLAELDDSAARAGGAAQPGSYV
                     VLSVIDSGIGMDEATKARIFEPFFTTKELGHGTGLGLATVYGIVQQHQGRLTVESEPG
                     RGTTFHVYLPATTETPEPAASERPTGLPGGAETVLLVEDETEVRELVRDMLRQLGYTV
                     LEAGNGPEAMEIGRRYRQPIHLLLSDVIMPHMSGRELAERMTHERPGL"
BASE COUNT          743 a         2126 c         1982 g          755 t
ORIGIN      
        1 cacgtccaga acctgctcgg caagctcgga gtccacagcc gccttcaggc ggtcgcctac
       61 gccacgacgc accgtcttct ctgagcgcgc ggcgctgacg atgtcgtcca tggccacgac
      121 ggccggcgcc agacgctttg cgacgctcgc gcggctcggt caggccgtga ccgcctcgct
      181 cgatctcggc gacgtgctcg acacggtcgc ccgcgcggcg accgacctgc tccggggctc
      241 gcacacggcc atctgggtcg ccgaggacga gcgcctggtg cggcgcgcgt cggccggcgg
      301 gcgcgagtcg tgtggcgaaa gctgcctccg cgagatcgcc gtgggcgagg gcctcatcgg
      361 tggcgtcgcc gccacccgca tgccgatggc cgtcgcggac gtcagcggcg agaacgactc
      421 gaccgggtgg gtccagcagc accagtacgt ctcattcctc gccatccccc tgctcgtcag
      481 ggaccggctc gtgggagtgc tctcgctggg cgcctggcac cgtcacgtct tctcgctgca
      541 cgagatggag gtgctcgggg ccttcggcga ccaggcggcc atcgcggtgg gcaacgccgg
      601 gctgtacgcc acgctcatcg cgcgcgtgcg acggctgcac gcgctcaccc ggatgacccg
      661 gctcatctcg tcgtcgctcg accccgacgc cgtgctccag gagatcgcgg acgccgcggc
      721 gagcttcggc gaggcccgcg tggcgtccat ctggagcgcc gatgacgtgg cgcgtgcgct
      781 cacgctcgtg gcctcgtccc gcgaggcgat gcgcgatttc ccgacgccga cgatggacta
      841 cgacgcgggc atcctcggct gggtagcgac gcggcgtgag gccgctcagg tctccgacgt
      901 cttcgccgac acccggttcg tggtgctcga ctggtggcgc gcccgcggcc tgtcgagctt
      961 cctcggcatc cccgtcgtgc tcgacgacga gctcgtggcc gtgctggcgc tccacggtga
     1021 ccgcccgctg gcgccgacgg cctccgagga gcgcgacttg ctcgagagct tcgcggcgca
     1081 ggcggccgtg gcaatccgca acgcccggct cttcaccgac agcgagcgcc gccggcgcgc
     1141 ggccgaggcg ctgcagcgga tcggccgcga cctcgcccac tcactcgacc cggaagaggt
     1201 cggtgcgcgg gtcgtcgcgt cggtcctcgg gctcatcggg gccaagcgcg catctctcta
     1261 ccggctggag gacgacagcg gcgacctcat cctgctcgcc accgcgggcg aggtgaagcc
     1321 cgccaacgac gtgctgcccc atggcgtcgg cacggccggt ctggccgcgg tgtcccgccg
     1381 gccggtcgcc tgctccgacg tccttgccga cgatcgcctc acgtggacgg ccgcggtgcg
     1441 cgcggccatc gagcccacgc gataccgggc ggtactgtca gtgcccctgc tcgtcaggtc
     1501 gcgcctgttc ggcgtcttgg ccatcgccga cgaggccggc cgcgccttca cgatcgacga
     1561 ggtgtcgctc gcgcaggcct tcgccgacca ggcagcgctg gcgctcgaca acgcccgcct
     1621 gctccacgag tcacgcacgc gacaggcgcg cctggagacc ctgctcgagg tcagccgcga
     1681 gctctcgggc atccagctgc tcgaccgcgt gctgaaccgg atcgccgagg cgtgcggacg
     1741 cgtcctcgac tcggggtcag tgggcttccg aatcgtcgaa gacgacgagc tggtggtccg
     1801 cggcgtctgg ggtgacgcgg gcgcggtcat ggccaagccc cgcctgaaga tcggcgagag
     1861 cctgtccggc cgcgtcgtgg ccaccggcca gccgctgatc gtccagcagc tcgccgacgc
     1921 gccggacctg atccccgaac accgcgagca ggccaggctc cacggcctgc gcgccttcct
     1981 cggcgtgccc gtcaacgtgg gcgagcgcgt ggccggcgtc ctgaccatcc gcagcctgga
     2041 cgaccgtgcc ttcggcgacg ccgagctggc cacggccacg gcgttcgccg cccaggcggc
     2101 ggtcgccctg cagaatgcgt cgctgtacgg cgagctccag gcgcagctcc ggcggaccga
     2161 ggcgctcctc aacgtgaccc gcgccgtctc gaccaccctc gatctcgacg aggcgatgcg
     2221 ccgggtcgcc cgcgagacgg cgcgcctgct cggcgccgac atggtgggcg cctacctggc
     2281 cgacgccgac ggcaagagcc tctggccagc cgcggggtac cgcgtgccgg ccgagctgcg
     2341 cgacgccttc cgacgccacc cgatcccgct cgccggccac gccttcgtcg aggacgcgtg
     2401 gcaccgccgg acgaccgtgt ggtcgagcga cgtcgagcgc gagccgggga tcgaccgcga
     2461 gacgatcgcg cggttccctc atcgcgcgct gctcttctcg ccgatggtcg cccagggccg
     2521 gtcgatcggc ggcctcttcg tcctgtggtg gaaggaccat cgtacgccga gcgccgagga
     2581 gatccgcctg ctcgagggtt tggccgatca ggccgcgctg tccttcgaga ccacgcgtct
     2641 ctacgagcag agcgagcgcg ggcggcgcgc cgccgaggcc ctgctcgagg tgggccaggc
     2701 gctcgcttcc acgctcgagc cggccgagat cttcacgctg gtcacgcggc gcacggcgca
     2761 ggccctcggg gccgagcgct gctccctcta cgagctgcgc cacggccgcc tcgtcccgct
     2821 catgtcccag tacgcggacg gccgcacgga caagacgctc tgggccctct tcaaaacgct
     2881 gccgcgtcac cggcccgacg acgtgcccgt gtacgccgag gccgtgagga cgcgccgtcc
     2941 ggtggtcgtc gcggacgcca ccgcgccagg ggccccggtc ccgccggagt gggtggacac
     3001 cttcgccatc cggtcgctcc tcgtcgtccc gctcttccac caggacaagc tcctcgggat
     3061 gctgaacttc gacacgacga tgccggcgcc gtggcggagg gagcagatcg acctggccat
     3121 gacgatcgcg aaccagacgg cgctcgcgat cgagaacgcg cgcctgttcg ccgcccagcg
     3181 cgaggaggcc caggtctcga gcgcgctgct gcgcctggcc cggtcgctgg agccgctgca
     3241 agacctgaac gcggtgctgg aggtcgcggt cgcgcgcgcg cccgagctgc tcggcctcac
     3301 gcgctgcagc gtcctcgtcg tcgatccctc gaccggtgag ctcgtgccca cggtggcgtc
     3361 gggtctcgcc gaggagcacc ggcccgcgtt ccaggcgctg cgcggcgtgg gcgccgtccc
     3421 cgccttcgca gaggccatcc gcagccacga ggtcgtggcc gtgcccgacg ccaccgagga
     3481 cacctggatc ccccgggcgg tcgcgcgcga gctcgacgtc caggcgatgc tcatcatccc
     3541 gctcgtcagc ggcggccggc tcatgggcgc gctcatcgtc gacacaccgg gccgcgctgc
     3601 cgcgttcgac gacaagcagc tcgccgtcgc gcgcggcatc gcctcgcacc tcgccggcgc
     3661 catcgaccgg gcgcagctgc acgccgagtc cgagcggcgg cgccgggagg ccgagatctt
     3721 cgcggagctg gcgcggacca tcaacgcctc gctcgatctg ccgacggtca tgcagcacgt
     3781 cgccgagtgg gcccgggagc tgtgccgctc ggacggggcg gctgcggccc tccgcgaggg
     3841 tgacgtgatg acgctccgct actgggtggg ccgcgacaac gtcggaccac tcacggtgga
     3901 gccgggcaag ggcgccggcg gcctcgtcat gaccacgggc cggccgttcc gcacggcgga
     3961 ctacgcgagc gacccgcgca tcacgaagga ctacgcggac atcgcccgcg gcctgggcgc
     4021 cgccgcccag ctggtggtgc ccatccacat cgccgaccag gtcgagggcc tgctctacgc
     4081 cggcaaccgc gcaccgcggc cgttcaccga gcgcgacgag gcgatcctgg tccgtctcgc
     4141 cgagcacgcc gcgatcgccc tcaagaacag ccgcctcttc gcccgagagc aggagtcgca
     4201 gcggttcctg cagtcgaccc tggacgcgct gtccgcgcac gtcgccgtgc tcgacgagac
     4261 cggcacgatc atcgccgtca acggggcctg gcggcgcttc gccgacgcgc atcgactggg
     4321 cgaccgcccc tacggcctcg gcgacaacta cctggccgtg tgcgaggccg cgcgcggcga
     4381 gggcgccgcc gaggccgccg ccgtcgccgc cgccatccgg accgtgctcc ggggcgagcg
     4441 cgccgagttc cacctcgagt acgactgtca cggcccgacc gaccggcgat ggttcgtcgt
     4501 gcgcatcacg cgcttcacgg ggccgggcct cgtccgggtc gtcgtggccc acgagaacat
     4561 ctccgagcgc cgcctggccg aggaggcgct gcagcgcaac gaggagcacg tgcgccaggt
     4621 gcagaagatg gaggccgtgg ggcggctggc gggcggcatc gcgcacgact tcaacaacct
     4681 gctcacggtc atcaccggac gcacccagct cgcgctccgg cgcctgcgcg aggacgatcc
     4741 catgcgccac gacctcgggc tgatccgcac gacggccgag cgcgcggcca cgctgacacg
     4801 ccagctcctc gcgttcagcc gcaagcagct cctccagccg cgggtggtgg aggtcgaggc
     4861 cgtcgtgcgc ggcctcgtcc cgatgctcca gcgcctcctg ggcgaggacg tggagctcat
     4921 catcccgccg gtggccggcc ccgcccgcgt caaggccgac cccggccagc tcgagcaggt
     4981 catcgtgaat ctcgccgtca acgcgcgtga cgcgatgccg cagggcggac ggctcaccgt
     5041 gcggatcggg ctcgcggagc tggacgacag tgccgctcgc gccggcggcg cggcgcagcc
     5101 gggctcgtac gtcgtgctct cggtgatcga ctccgggatc ggcatggacg aggccacgaa
     5161 ggcgcggatc ttcgagccgt tcttcaccac caaggagctc ggccacggca cggggctcgg
     5221 cctcgcgacg gtctacggca tcgtgcagca gcaccagggc aggctgacgg tcgagagcga
     5281 acccgggcgc ggcaccacgt tccacgtcta cctcccggcc acgaccgaga cgccggagcc
     5341 ggcggcgtcc gaacgcccca cgggcctgcc ggggggcgcg gagaccgtgc tgctggtcga
     5401 ggacgagacc gaggtgcgcg agctcgttcg cgacatgctc cggcagctcg gctacacggt
     5461 gctggaggcc ggcaacggcc ccgaggcgat ggaaatcggc cggcgctacc gccagcccat
     5521 ccacctgctg ctgagcgacg tgatcatgcc gcacatgagc ggccgcgagc tggccgagcg
     5581 catgacgcac gagcgccccg gcctgc
//
LOCUS       DATIAZ010000283         6106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1397038, whole genome shotgun sequence.
ACCESSION   DATIAZ010000283 DATIAZ010000000
VERSION     DATIAZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397038"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VLF19_09950"
     CDS             complement(<1..299)
                     /locus_tag="VLF19_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSE93617.1"
                     /translation="MATLPDVLDKLTPEARRVYDRIKAKRGALRGPYAPLMHHPELAE
                     QVGDLGEYLRFDSRLPGDVRELAILITARSVSQAYEWTAHAPIGRKAGLPDDVIER"
     gene            complement(448..876)
                     /locus_tag="VLF19_09955"
     CDS             complement(448..876)
                     /locus_tag="VLF19_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93618.1"
                     /translation="MSARLFDLLGALDGWALVVVVAAWVAATVLTLGIVALVVVTLPA
                     TYFVAEAPAIRAPRGSAGWLWLLVRNLLGVALILLGMLLSLPGIPGQGLLTMLIGVML
                     VDFPGRRRLERALARRPSVLAALNRLRARWGKPPLLAPAG"
     gene            1105..2250
                     /locus_tag="VLF19_09960"
     CDS             1105..2250
                     /locus_tag="VLF19_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE93619.1"
                     /translation="MRLIVLFCLSGFAGTFSIGAFPALLPEIGREQGLADWQLGVIAG
                     AFGFARMCADLPVGMFITHHLRRALVAAPLLITLGILALGSGGPLPLLIAGRAVMGVA
                     HALNMVAALTAILRYQAAGRLASALSAFEFSAMLGILGGTAALGFVPERVPWNLALMI
                     TSAPQILGIAIAPVVLAALPREAVPASRPLFARRPHGGGAHAPATAAVALAFVAGGVI
                     AIAYSTLEQFVIPLRASREFDMPRAGIARLLMTVQVCDIACLLPAGVLADRHGTSRVL
                     GTMLVVFGVAAALVTFGDFVLLAVGCALFGIGMASWTLPLSLLRRETPPEQVAWRTAL
                     YRVGVDGGIFLGPFLSGLLIGRAPGVLPGVLAVVLAVVGVTLARRRA"
     gene            complement(2162..2719)
                     /locus_tag="VLF19_09965"
     CDS             complement(2162..2719)
                     /locus_tag="VLF19_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE93620.1"
                     /translation="MTIVRATRRDAATILGLVRGLAEYEHLSHEMRATAGDLRRHGFG
                     RRRFFEALICRRGRRPIGFGLYFFTYSTFEGRPTLYLEDLFVLPAERGRGAGRALLRA
                     LARIAIRRGCGRMEWAVLDWNTPSIRFYRRLGAGLRREWVLTRLTGAPLRRLAGLTRA
                     GARASRRRPPARRRAHPAGPRARGR"
     gene            3318..4949
                     /locus_tag="VLF19_09970"
     CDS             3318..4949
                     /locus_tag="VLF19_09970"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSE93621.1"
                     /translation="MTGGEALVAQLVREGARVVFGLPGVQLYGVVAALRDEPAIRLIT
                     TRHEQATSYMADGYARGGGDFGVALVVPGPGLLNASAGLSTAYACSSPVLMISGQVPK
                     ASLGKRIGVLHEVDDQLDCVAPVTKWRRRVLEVAEVPAAVHEAVRQLKTGRPRPVEIE
                     MPPETMEDEGEAELVGADHPSRPAAPRREVDRAAEVLLAAERPVIYAGGGVHLSGAHE
                     AVAAVADYLQAGVIESAEGKGAVSHESEIALGAALAWQGPLRRHLLAADVILAVGTRF
                     ALAAARPEQQVVQIDIDPEEIGRNHARTLGLPGDARATLERLLERLRATAPPRPSRKT
                     EYNALRAEVAATDAQEPASSIVRTLRAAVPEETIVVTDMTQIGYYSRPFWPVYRPRTF
                     LTSSYSGNLGFAYPTALGAKMACPDRPVIAVCGDGGFMYNVQELSTAALHGIAVVAVV
                     FNDGAYGNVARDLDEAWGGSYAAELRNPDFMRLADAFGVPGLRAKEPGQVGTLVREAL
                     AAGRPALVEVPVGRMPRPSFFLPRRKPTKYQGLAG"
     gene            4975..5877
                     /locus_tag="VLF19_09975"
     CDS             4975..5877
                     /locus_tag="VLF19_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE93622.1"
                     /translation="MSELDAVRQSYAEEIRAVSNVRSAAVVQAFATIPREWFLGPGPW
                     QILAPVPPGCFPYRTTDDADPRHLYHNVLVAIDAARRLNNGQPSFLAFCLDSLDLQGG
                     DHAVHVGCGVGYYTAILAAVVGQQGRVTAIELDRVLAARARENLAHLKHVAVVAADGS
                     EHDPGPSDAIFVNAGATHPRSMWLDSLRPGGRLLVPLTVAIDASGHGRGGILKVTRRP
                     GGLEARFISEVGIFPCIGARDPHANELLQAAFRRHTWESVQSLRRDPHDATERCWLHL
                     RGVCLSTVPVPRDGATPVDGGAMS"
     gene            5926..>6106
                     /locus_tag="VLF19_09980"
     CDS             5926..>6106
                     /locus_tag="VLF19_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93623.1"
                     /translation="MSRRVVRSYEARYSMGVNKATMYDLTDGIDVHAHARGGDEEDPL
                     HAAREATRAGMKAIVF"
BASE COUNT          833 a         2188 c         2234 g          851 t
ORIGIN      
        1 cgctcgatga cgtcgtcggg caggccggcc ttgcgtccga tcggcgcgtg cgccgtccac
       61 tcgtaggcct ggctcaccga gcgcgccgtg atgaggatcg ccagctcgcg gacgtcgccc
      121 ggcagccgcg agtcgaagcg caggtactcg ccgaggtcgc cgacctgctc ggccagctcc
      181 ggatggtgca tgagaggagc gtaggggccg cgcagggcac cccgcttggc cttgatgcgg
      241 tcgtacacgc gccgggcctc gggagtgagc ttgtccagga cgtcgggcaa cgttgccatc
      301 aggcacacct ctggacggcg ccggacccgg cgccgggatc ggggtcgaag gcaccaggat
      361 cggttgcgca ccggcggcgc gcgcatccga tcgcacgatc ggacatggcc ggagtgagga
      421 tagcagagac cggggcgagg gcgcgcatca gccggcgggc gccaggaggg gcggcttgcc
      481 ccaccgcgcc cggagacggt tcagcgcggc cagcacactg ggccgccgcg ccagcgcgcg
      541 ctcgagccgc cgccggccgg gaaagtccac cagcatgacc ccgatgagca tcgtcagcag
      601 gccctggccc gggattccgg gaagcgacag gagcatcccg agcaggatga gggcgacgcc
      661 gaggaggttc cgcaccagga gccagagcca cccggcggag ccgcggggcg cgcgaatcgc
      721 gggcgcctcg gcgacgaagt acgtggcggg tagggtcacc acgacgagcg ccacgatgcc
      781 gagcgtgagc acggtggccg cgacccacgc cgcgaccacg acgaccagcg cccagccgtc
      841 gagagccccg aggagatcaa acagacgcgc ggacataccg tcgtcgccgt cattatcacc
      901 cggggaacgg gagaggacag cgaatcacgc gagcgtcgac gacccgccgc cgttacgggc
      961 tccgcaccgt acggcgcccg gatcgagatg ttctcccact ccacgccact cacgaccctc
     1021 gccgggcggt ccaggataga cgaggcgtgc gccggcttca ccgggcgtcc gtcatgccgg
     1081 cctccggcca tacaatcgct cctcgtgcgc ctgatcgtcc tgttctgcct ctcgggcttc
     1141 gccggcacgt tctccattgg cgccttccct gcgctcctgc cggagatcgg gcgcgaacag
     1201 gggctcgcgg actggcagct gggcgtcatc gccggagcct tcggcttcgc gcgcatgtgc
     1261 gccgacctgc cggtcggcat gttcatcacc caccacctcc gccgggcgct ggtcgccgca
     1321 ccgctgctga tcacgctcgg catcctcgcg ctcggcagcg gcgggccgct gccgctcctg
     1381 attgccggcc gcgcggtgat gggcgtcgcc cacgcgctca acatggtggc cgcgctgacc
     1441 gcaatcctga gataccaggc ggcggggcgg ctcgcctcgg ccctgagtgc cttcgagttc
     1501 tcggcgatgc tcggcatcct cggcggcacc gcggccctgg ggttcgtgcc cgagcgcgtg
     1561 ccgtggaacc tggcgctgat gatcacgtcc gcgccgcaga tcctcgggat cgcgattgcg
     1621 cccgtcgtgc tggcggcgct gccgcgtgag gccgtgccgg cgtcgcgccc gctcttcgcg
     1681 cgccgtcccc acggcggcgg cgcgcacgcg ccggccacgg ccgcggtggc cctggccttc
     1741 gtcgccggcg gggtcatcgc gatcgcgtac tccacgctcg agcagttcgt gatcccgctg
     1801 cgcgcgagcc gcgagttcga catgccccgt gccggcatcg cgcggctgct gatgaccgtg
     1861 caggtgtgcg acatcgcgtg cctgctgccc gccggcgtgc tcgccgaccg ccacggcacc
     1921 tcgcgcgtgc tcggaacgat gctcgtggtc ttcggggtgg ccgcggcgct ggtgaccttc
     1981 ggcgacttcg tgctcctggc cgtgggctgc gcgctcttcg ggatcgggat ggcgagctgg
     2041 acgctgccgc tcagcttgct ccggcgcgag acgccgcccg agcaggtggc gtggcggacg
     2101 gcgctctatc gggtcggcgt ggacggcggt atcttcctcg gcccgttcct ctccggcctg
     2161 ctcatcggcc gcgcgcccgg ggtcctgccg ggtgtgctcg ccgtcgtgct ggcggtcgtc
     2221 ggcgtgacgc tcgcgcgccg gcgcgcgtga ggccggcgag gcggcggagc ggcgcgccgg
     2281 tgaggcgcgt gaggacccac tctcggcgca ggccagctcc gagccgtcgg tagaaacgga
     2341 tcgacggggt gttccagtcc aacacggccc actccatgcg cccgcacccg cggcggatgg
     2401 cgatccgcgc gagcgcccgc agcagagcgc ggccggcgcc gcggccgcgc tcggcgggca
     2461 ggacaaagag gtcctccagg tacagcgtgg gacggccctc gaacgtggag taggtgaaga
     2521 aatagagccc gaagccgatg ggccggcgtc ctcgccggca gatcagcgcc tcgaagaagc
     2581 gccgccggcc gaacccgtgg cggcggaggt cgccggcggt cgcgcgcatc tcgtgggaaa
     2641 ggtgctcgta ctcggcgagg ccgcggacga gaccgaggat cgtcgccgcg tcgcggcgag
     2701 tggcgcgcac gatggtcagg ccccgggccg tcacgggcgc cgatcatagc accggcccgt
     2761 gccggtctct cgagagtcag gatcgcgtcc tctcggcgcg cgggtcatcg aggcgcgcca
     2821 cgccgtcgcg atgtcggaga tcgcacagcc cggggatgga cgtcgcgctc ccacttcgac
     2881 gtcgaggggg acgccggccg gcggacatcg aggagccgta gaatgtcggc ccgggggaag
     2941 ccgccatgac cctggaccgg atcattgagc tggtggggcc gctcgacgac tctccgggcg
     3001 aaagcaccgc gagggaccgc ttcccacgca cctcgcgact cagtcaccgc accaggcgcg
     3061 ctccggacca cagcgagatg tgcccatgcg cccggtcgca ggggcatgag cggctctgca
     3121 aacgctattg tcggtgccgc ggcgatcggg ggcgccgccg gcggtcgggg tgtgcacggc
     3181 ccagtgcgcc cgatcggctg gaaccgtcat ttcacggtgc actcgggagc ggtcagaata
     3241 tgtatcgcca acacgagggc cccatgtaat ttgacggcgg gcgacgcgcg ggctaccctc
     3301 ggcatcccat ggcgcgcatg accggaggcg aggccctcgt agcgcagctc gtgcgcgagg
     3361 gcgcgcgcgt cgtcttcgga cttcccggtg tgcagctcta cggcgtcgtg gccgcgctgc
     3421 gggacgagcc ggcgatccgg ctgatcacta cccgtcacga gcaggccacg agctacatgg
     3481 ccgacggcta tgcccgcggg ggcggcgact tcggggtcgc tctcgtcgtc cccggccccg
     3541 gcctcctgaa cgcgtcggcc gggctcagca ccgcctacgc gtgttcgtcg ccggtgctca
     3601 tgatctcggg ccaggtgccg aaggccagtc tcggcaagcg catcggcgtg ctgcacgagg
     3661 tcgacgatca gctcgactgc gtcgcgccgg tgaccaagtg gcgacgccgc gtgctcgagg
     3721 tggccgaggt gcccgccgcc gtccacgagg ccgtgcggca gctcaagacg ggccggcccc
     3781 ggccggtcga gatcgagatg ccgcccgaga cgatggagga cgagggcgag gccgagttgg
     3841 tgggcgccga tcacccgtca cggccggcgg cgccccggcg cgaggtggac cgggccgccg
     3901 aggtgctgct cgctgccgag cggcccgtga tctacgccgg cggcggcgtg cacctgtcgg
     3961 gcgcgcacga ggccgtcgcc gcggtcgccg actatctgca ggccggcgtc atcgagagcg
     4021 ccgagggcaa gggcgcggtg agtcacgaga gcgagatcgc gctgggcgcg gccctcgcgt
     4081 ggcaggggcc gctccgccgt catctgctcg cggccgacgt catcctcgcc gtcggtacgc
     4141 gcttcgcgct ggcggcggcg aggcccgagc agcaggtcgt gcagatcgac atcgatcccg
     4201 aggagatcgg ccgcaaccac gcgaggacgc tcgggctccc cggcgacgcg cgcgcgacgc
     4261 tcgagcgcct gctcgagcgg ctgcgcgcga cggcgccgcc ccggccctcg cggaagaccg
     4321 agtacaacgc gctccgcgcc gaggtcgcgg ccacggacgc gcaggagccg gcgtcgtcga
     4381 tcgtgcgcac gttgcgcgcg gccgttcccg aggagacgat cgtcgtcacc gacatgaccc
     4441 agatcggcta ctactcgcgg ccgttctggc ccgtgtaccg gccgcggacg ttcctgacct
     4501 cgtcctactc cggcaacctc ggcttcgcct atcccacggc gctcggcgcc aagatggcgt
     4561 gccccgatcg gcccgtgatc gcggtgtgcg gtgatggcgg gttcatgtac aacgtgcagg
     4621 agttgtccac tgcggcgctg cacggcatcg ccgtcgtcgc cgtcgtcttc aacgacggcg
     4681 cgtacggcaa cgtggcgcgc gacctcgacg aggcgtgggg cggcagctac gcggccgagc
     4741 tccggaatcc cgacttcatg aggctcgccg acgccttcgg cgtgccgggg ctgagggcga
     4801 aggagcccgg acaggtcggg accctcgtgc gcgaggcgct cgccgccggt cggcccgcgc
     4861 tcgtcgaggt gcccgtcggc cggatgccgc gcccgtcgtt cttcctcccg cgtcgcaagc
     4921 cgaccaagta ccagggcctc gccggttgag cggccccacc gcgacgcgcg ggcggtgagc
     4981 gagctcgatg cggtccggca gagctacgcc gaggaaatcc gtgccgtctc gaacgtgcgc
     5041 tcggcggcgg tcgtgcaggc gttcgcgacc attccgcgcg aatggttcct cgggcccggg
     5101 ccgtggcaga tcctcgcgcc cgtgccgcca gggtgcttcc cctaccggac cacggacgac
     5161 gcggacccgc ggcacctcta tcacaacgtg ctcgtcgcga tcgacgcggc ccggcgtctc
     5221 aacaacggac agcccagctt cctggccttt tgcctcgatt ccctcgacct ccagggggga
     5281 gatcatgcgg tgcacgtcgg atgcggggtc ggatactaca cggccatcct ggctgccgtc
     5341 gtcggtcagc agggccgcgt caccgcgatc gagctcgatc gggttctcgc ggcccgcgcg
     5401 cgcgagaacc tggctcatct gaagcacgtc gcggtcgttg ccgccgatgg cagcgagcac
     5461 gaccccgggc cgagcgacgc catcttcgtg aatgccggcg ccacgcatcc ccggtcgatg
     5521 tggctggatt cgctccggcc cggggggcga cttctcgtcc cgctcaccgt tgccatcgac
     5581 gccagcggcc acggccgcgg cggcatcctg aaggtcacgc ggcggccggg cgggctcgag
     5641 gcgcgcttca tctccgaggt cggcatcttc ccgtgtatcg gggcgcgaga cccgcacgcg
     5701 aacgagctgc tccaggcggc attccgccgg cacacctggg agtcggtgca gtcgctgcgg
     5761 cgagatccgc acgatgcgac ggagcgctgc tggctccacc tccggggcgt ctgcctctcc
     5821 accgttccgg tgccgcgcga cggcgcgacg cccgtcgacg gcggggcgat gtcgtgagat
     5881 agtcgggagc ctcgaacgac cgaaccccca ggtgaggcgc ctatggtgag ccggcgagtg
     5941 gtcaggtcct acgaagctcg atacagcatg ggcgtcaaca aggcgaccat gtacgacctc
     6001 acggacggga tcgacgtgca cgcccacgcc cgcggcggtg acgaggagga tccgctgcac
     6061 gccgcgcgag aagcgacccg ggccggcatg aaggcgatcg tcttca
//
LOCUS       DATIAZ010000284         3924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_871240, whole genome shotgun sequence.
ACCESSION   DATIAZ010000284 DATIAZ010000000
VERSION     DATIAZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871240"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VLF19_09985"
     CDS             complement(<1..575)
                     /locus_tag="VLF19_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93624.1"
                     /translation="MTLAGLLERAALRHPDAEAVVDGGARLTYATLDARARSLGRTFA
                     RLGVGRGDRVLIVLKNRLEHVLAYWALQTIAGVPVPVNFRLAAGEIAYVLADSGARLV
                     LFEPATATAVLEATRDRDVTLIFVGRPDAKLTNRPDILPFDSLEEPQRGTGGGGEGGG
                     EEPLTCSPPPVTRETRRWTDATMDLAHRIRG"
     gene            complement(572..1600)
                     /locus_tag="VLF19_09990"
     CDS             complement(572..1600)
                     /locus_tag="VLF19_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSE93625.1"
                     /translation="MKAAYFKEHGGADKILHGDYPDPVPEAHEVVVRVRACALNHVDM
                     LLLDGRFPPPEGLPHVNGCEVTGTLEAGGATVTGLAPGRRVIVFPGFSCGQCEFCLRG
                     ERTVCLRYGYLGAHKNGGYAELVKVPAANVLPLPDAVSFEQGAAVPLAMLTSWHALVA
                     RAGVRPGQTVLVQAAGSGVGSAAIQIARLCGARVMTTVGSDDKRELARALGAEHVVNY
                     RTQDFVEEAKRWTGKRGVDVVVEHIGGETFERSVYALGRLGTLITIGSHDTHWGRLDL
                     RHVYSKNLRVLGTNLGTILELGQVLDHLVDGRLQPVIDRTFPLRDAAAAVQYMVDRKN
                     RGKVLLVV"
     gene            complement(1597..2040)
                     /locus_tag="VLF19_09995"
     CDS             complement(1597..2040)
                     /locus_tag="VLF19_09995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HSE93626.1"
                     /translation="MKIEGSHDVPAPRQKVWEAFLDPERLRQAIPGCEKLEALGNDEY
                     RAVMKIGVAAVKGTFEGKVRIADKNPPESYRLNAEGSGGPGFVRADTVIVLSDVEGGT
                     RVSYNADVQVGGLIAGVGQRMLGGVSRMMADQFFSRMAQILTEAA"
     gene            complement(2482..3363)
                     /locus_tag="VLF19_10000"
     CDS             complement(2482..3363)
                     /locus_tag="VLF19_10000"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HSE93627.1"
                     /translation="MRKLRDAFGDERWRAVFEAAEDAAIVMVIGESDTGKTTLVTALA
                     NAAHARGRTVGIVDADLGQSEIGPPTTIGVGRVRAPLRRLGDAEVLALEFVGATSPAR
                     NVLGAVVGIRRALDRARAARLDRIVVDTSGLVAGSLGRVLKQAKIDVLDPDLVVCLER
                     GVECQAIVAPYLAGRRPRVLRMRVAPAVRVRTAEERRRHREERLRAHLVSARPVALDL
                     GRILVRLPGGDVVAAPEALGEHVGAIAGLEDEARQTLGLAVVRGLDPTGTALHIETTV
                     DVARVAGVRIGRERYEG"
     gene            complement(3559..>3924)
                     /locus_tag="VLF19_10005"
     CDS             complement(3559..>3924)
                     /locus_tag="VLF19_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSE93628.1"
                     /translation="VEALAGPQDEVAVMAAEFDRRRHLVVEGLNALPGVRCVMPKGAF
                     YAFANVSGLFGRVVPGTGKKLGGSADVTEFFLEHARVAVVPGVDFGSDAHVRLSYAAS
                     TEAIREGIARMDAAIRTLG"
BASE COUNT          558 a         1471 c         1358 g          537 t
ORIGIN      
        1 tgacccctga tgcggtgcgc gaggtccatc gtcgcgtcgg tccatcgacg cgtttcgcgc
       61 gtaaccggcg gcggggaaca ggtcagcggc tcctctccac cgccctcccc gcccccgccg
      121 gtcccgcgct gtggttcttc caaggagtcg aacgggagga tgtcggggcg gttcgtgagc
      181 ttggcgtcgg ggcggccgac gaagatcagg gtcacgtcgc ggtcgcgggt ggcttcgagg
      241 acggcggtgg ccgtcgccgg ctcgaacaat acgaggcggg cgccggagtc ggcgaggacg
      301 taggcgatct cgcccgcggc caggcggaag ttcaccggga ccggcacgcc ggcgatggtc
      361 tggagcgccc agtacgcgag gacgtgctcg aggcggttct tcagcacgat gagcacgcgg
      421 tcaccgcggc cgacgccgag tcgcgcgaac gtccgtccga gcgagcgagc gcgcgcgtcg
      481 agcgtggcgt aggtcaggcg cgcgccgccg tccacgaccg cctcggcgtc ggggtggcgc
      541 agcgcggcgc gctccaggag gccggcgagc gtcatacgac caacagcacc ttgcctctgt
      601 ttttccgatc gaccatgtac tggactgctg ccgcggcgtc acggagcggg aacgtgcggt
      661 cgatcacggg ctggagtcgg ccatccacga ggtggtcgag aacctgcccc agctcgagaa
      721 tggtcccgag gttcgtgccg aggacgcgca ggttcttcga atagacgtgg cgcaggtcga
      781 gtcggcccca gtgggtgtcg tgggagccga tcgtgatcag cgtccccagg cggcccagcg
      841 cgtagaccga gcgctcgaac gtctcgccgc cgatgtgctc gacgacgacg tcgacgccgc
      901 gcttgcccgt ccagcgcttg gcctcctcca cgaagtcctg cgtccggtag ttgacgacgt
      961 gctcggcgcc gagcgcgcgg gccagctcgc gcttgtcgtc gctgccgacg gtcgtcatca
     1021 cgcgcgcgcc gcagaggcgc gcgatctgga tggccgcgct gccgacgccg ctacccgccg
     1081 cctgcacgag gacggtctgc ccggggcgca ccccggcccg ggccaccagc gcgtgccacg
     1141 acgtcagcat cgccagcggc accgccgcac cctgctcgaa cgagaccgcg tcgggcagcg
     1201 gcagcacgtt ggccgccggc accttcacca gctccgcgta gccaccgttc ttgtgcgcgc
     1261 cgagatagcc gtagcggagg cacaccgtac gctcgccgcg gagacagaac tcgcactgac
     1321 cgcacgagaa gccggggaac acgatcaccc gccggcccgg cgcgagcccc gtcacggtgg
     1381 cgccgccggc ctcgagcgtg ccggtcacct cgcagccgtt cacgtgcggc agcccttcgg
     1441 gcggaggaaa gcgtccgtcg agcagcaaca tgtcgacgtg gttcagggcg cacgcgcgca
     1501 cgcgcacgac gacctcgtgc gcttcgggga ccgggtcggg gtagtcgccg tgcaggatct
     1561 tgtcggcgcc gccgtgctcc ttgaaatagg cggccttcac gcagcctccg tcaggatctg
     1621 ggccatgcgg ctgaagaact ggtccgccat catcctgctg acgccgccga gcatgcgctg
     1681 accgacaccg gcgatgagcc cgccgacctg gacgtcggcg ttgtaggaca cgcgcgtgcc
     1741 gccctccacg tcgctcagca cgatgacggt gtcggcgcgg acgaagcccg ggccaccgct
     1801 gccctcggcg ttcaggcgat acgactccgg cgggttcttg tcggcgatcc tcaccttgcc
     1861 ctcgaacgtg cccttgacgg ccgccacgcc gatcttcatc accgcccggt actcgtcatt
     1921 gccgagcgcc tcgagcttct cgcaaccggg gatcgcttgc cgcaagcgct cggggtcgag
     1981 aaaggcctcc cacaccttct ggcggggcgc cggaacgtcg tgcgagcctt cgattttcat
     2041 ccgcgctctc ctcgagggtc gagtcggggg tgcgtgaggc gccctagtgt accaaggccg
     2101 acgttccgtg gcactggcat tgccgcggtc acctgaggct ccaacgccga gcacaggagg
     2161 accgcaatga gaagattgct cacgggtgtg tgcgccctgg ccctcggggc cggtgtgctc
     2221 atcggctgca ccgagtctcg gagcgaccgg gtcggcgagc gaccgggtga gcggacgccg
     2281 tccgcgtcac cacgaaccac cacgacgccg tcgccctcgc ggacgccgga cgccacgacg
     2341 tcgccgagca ccagcccggc gacgccgccg gcgggcacgc cgccggggag cggcacgaag
     2401 taagcaccgc gtcgcgtctg tcgtcggagg cccgatcgcc ggtgcgcact ccgcgcaccg
     2461 gcgatcgggc tctccgcggt ctcacccttc gtaacgctcc cgcccgatcc gcaccccggc
     2521 cacgcgcgcg acatcgaccg tggtttcgat gtgcagcgcg gtgcccgtgg ggtcgaggcc
     2581 tcgcacgacc gcgagtccca gtgtctgccg cgcctcgtcc tcgagtcccg cgatcgcccc
     2641 gacgtgctcg ccgagcgcct ctggcgcagc caccacgtct ccgccaggca atcgcaccag
     2701 gatccgcccg aggtcgagcg cgaccgggcg cgccgacacc aggtgcgccc gaagccgctc
     2761 ctcgcggtgg cgccgccgct cctcggcagt gcgcacgcgc acggccggcg cgacccgcat
     2821 cctgagcact cgcggccgcc tgccggcgag gtacggcgcg acgatcgcct ggcactccac
     2881 tcctcgctcg aggcagacga cgagatccgg atcgagcacg tcgatcttcg cctgcttcag
     2941 cacgcgaccg aggcttccgg cgacgaggcc gctcgtgtcc acgacgatcc ggtcgagccg
     3001 cgcagcgcgc gcccggtcga gcgcgcgccg gatgccgacg acggcaccga ggacattgcg
     3061 ggcaggcgag gtggcgccga cgaactcgag cgcgagcacc tcggcgtcgc cgagtcgccg
     3121 gagcggcgcg cgcacgcgcc ccaccccgat cgtggtcggc ggcccgatct ccgactgccc
     3181 caggtcggcg tcgacgatgc ccaccgtccg cccacgcgcg tgagcggcat tggcgagcgc
     3241 ggtgacgagc gtcgtcttgc ccgtatcgct ctcgccgatc accatgacga tggccgcatc
     3301 ctcagccgcc tcgaagaccg cgcgccaccg ctcgtcgccg aatgcatcgc gcaacttccg
     3361 cacgcggcca tcctaactca cgcgcgttgt cgcgcggggg gccgggggag ggcggcggag
     3421 cggggcccat gacctgtttc ccgccgccca tccccgcgca agacgcagcg atggacccac
     3481 gcaacgatgg acgtcgcgcc gcgcgcccgg ggtcagggcc ccgctccgcc gccctccccc
     3541 ggccccccgc gcgccgcctc agccgagcgt gcggatggcg gcgtccattc gcgcgatacc
     3601 ttcgcggatc gcctcggtgc tggccgcgta cgagagccgc acgtgcgcgt ccgacccgaa
     3661 gtcgacaccc ggcaccacgg ccacgcgcgc gtgctcgagg aagaactcgg tgacgtcggc
     3721 cgagccgccg agcttcttgc cggtgccggg gacgacgcgg ccgaagagtc ccgagacgtt
     3781 cgcgaatgcg tagaacgccc ccttcggcat cacgcaccgc acgccgggca gcgcgttgag
     3841 gccttcgacg acgagatgcc gccgccggtc gaactcggca gccatcacgg ccacctcgtc
     3901 ttggggtccc gccagcgcct ccac
//
LOCUS       DATIAZ010000285         6232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1134454, whole genome shotgun sequence.
ACCESSION   DATIAZ010000285 DATIAZ010000000
VERSION     DATIAZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134454"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..480
                     /locus_tag="VLF19_10010"
     CDS             268..480
                     /locus_tag="VLF19_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93629.1"
                     /translation="MAARKKTRKTVMPSPRSAKAGRPPREVPASSQVRRRRKTATAAV
                     RKDSMVQQTAEARIWWGVGKPKEKKR"
     gene            574..1899
                     /locus_tag="VLF19_10015"
     CDS             574..1899
                     /locus_tag="VLF19_10015"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSE93630.1"
                     /translation="MAHRLRLDVGMKNEEKPPAVGDLPPSGNGHGASAFDVRGVASAP
                     EAAAARSDRLRRWLPLGVLALAALLLVALLAPRLVSSLRSPAPAGLLFASGRIEGRIT
                     TLTPKSSARVTTLHIDEGQPVRAGDLLATLDDRAQRERVNAAQQYLDVLVERLRAADT
                     ELAMTERQVSLQIEQAEAALREADARLQRARTSELQARRDAQRAAILVAKELIAAQVA
                     EHARLQADLEVEAVREAEEALARTRKQLEQARLGPQQVAARRAERDALERQRRQAQAQ
                     LAEQASVVTDFAIRSPIAGRVLTRTIEVGERVEAGTPLFTLVDLDRLYVKIYVPEPSI
                     GKVALGQEARVYVDAHPGRPFAARVSKVAQEAEFTPKNVETREERVKLVFAVEVALAE
                     NPGGVLKPGMPADAVIRWQPDAAWVTPADASRRGVLRREGATRPPGETR"
     gene            1914..3698
                     /locus_tag="VLF19_10020"
     CDS             1914..3698
                     /locus_tag="VLF19_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93631.1"
                     /translation="MQRLDGEAAAIDARRLSKTYRRGRVHAVRDVSLRVAAGEVFGLI
                     GPDGAGKTSVLQILAGVLSIDGGEARVAGVDVARDPERVKALIGYMPQGLGLNLYDAL
                     TVEENIEFFRALRRVPADRYRDNRDRLLRMTRLAPFLDRPARKLSGGMRQKLALICTL
                     IHLPDVLLLDEPTTGVDPLSRRDFWTIIHTLVATRGVTVLLTTSYMDEAERCHRVSLM
                     HEGALIAGGTPAALAASLAGPVPGTLEDVFVHHLTAGRPLPDVGSRLAPAREGAASTG
                     NGGRAAGGAPVVANGLTCRFGAFTAVDAVSLEVHQAEIVGLLGPNGAGKTTLIKMLCG
                     LQPPTAGSASVAGYDVGRQGRDVRRSIGYMSQRFSLYRDQTVLENLGLSAGLYGIRGS
                     RGRSRIEALLASLGLAPFADRLPAALPLGLRQRLALACAILHEPRVLFLDEPTAGVDP
                     LARRDFWNLVHQLAHEDGVAVLVSTHYMDEVTHCDRLGFMHEGRLVGLGRPEELAKEA
                     ERHGGPMVAVETPTFDRAFVLLRDQFPAAMLYGRRIRWQSVQPDHDIHRARQVLAAAG
                     IEASVGTQPLSMEDTFVSVLRVAGLSHA"
     gene            3691..4824
                     /locus_tag="VLF19_10025"
     CDS             3691..4824
                     /locus_tag="VLF19_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93632.1"
                     /translation="MLDRTLAIARKEARELLRDPAYLGLAFVVPLLLLLVLGHQLSVD
                     VKGLLVAVVDRDRSPWSRQYVDGFVHSEYFRLVGFLDGEAEAREWIRSGRARVVIDIP
                     PDFGRRLSGGERATVGVTVDGSFPTRGQIALGYVEAIGALHNERLAQAFFAERGRRDG
                     PIWPVQMDVSVWYNPTLESKNFIVPGMLVVILMIFPPLLTSLVIVRERESGTILNLYC
                     SPASRWDIVAGKALPYAAVSFVDYLVIFAASVWIFQVRFVGSVWLLSAGAVLYSICTV
                     GLGLLISAFTRTQLAAVLATFLVAVAPAFTFSGIFAPVASQDAVGQGVSYLIPATYFM
                     DVVRGSFLKGGGIATHGARLLALAAYAVVVYTTAAFLVRKRVG"
     gene            4824..5945
                     /locus_tag="VLF19_10030"
     CDS             4824..5945
                     /locus_tag="VLF19_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93633.1"
                     /translation="MASRLAAMIRKEFSQLFRDLPMLLILLWAFTGAVYTGGHGMSTE
                     LQNHPIAILDLSRSAESRELVSRFRAPHFRVAAYAASDAQLVALLDAGRVSLGIVIPP
                     DFARQAHDAGARFQVIVDGSQSTLATIAGAHIASIASGYGVDFLEGRSGGRVGEGLPR
                     VDARIRVEYNPNLQERWFSSVLEVLNQVTMIAMLLAAAAMVREREQGTLEYLLVSPLR
                     PVELFATKAVPVVTLVPLAALGALFGIVQGVFGTPIRGSVVLFYAVSVVYVFAMASLG
                     LAIAVVARTLGQAVLLLLLVFYPMVLLSGAFTPPESMGAAMRYAGLLSPARHYVDFGY
                     QVLFKGNGLAYVWPQVAGILALGLVVFAISVRRFARLVR"
     gene            6037..>6232
                     /locus_tag="VLF19_10035"
     CDS             6037..>6232
                     /locus_tag="VLF19_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93634.1"
                     /translation="MNVWHRRCTDLRERAGSVPAHEGGAGLGAPGPSREELLARAAQP
                     GEEALRLHALCGGKMQTLPKC"
BASE COUNT          828 a         2214 c         2236 g          954 t
ORIGIN      
        1 caacgcggga gggacgctga gcgtcggcgg catgctcttc gtcaatcgca cgacgtgggc
       61 gcactgcctc gacgccgtcg cggtcgtctg cggcctggac agagcggcgc tgttgagccc
      121 cgacgagatc gccgcgctcg atcaccgcgc ctcgccgcac ggcgtcctga tctgagcgcc
      181 tggcggtcac gtgcgtacga ggtcggtgcc ctggcgcggc gagccagaac gagccgtact
      241 ccgccgtcat cgcagagagg agccgtcatg gcagccagga agaagacgcg caagaccgtg
      301 atgccgtccc cgcgctcggc caaggccggg cgcccgccgc gcgaggtccc cgcgtcatca
      361 caggtccggc gccggcgcaa gacggcgacg gcggcggtcc gtaaggacag catggtgcag
      421 cagaccgcag aggctcgcat ctggtggggc gtcggcaagc caaaggaaaa gaagcgctga
      481 cggctctggg gcgtcgctgc cccaccccgg gggtcgtacc ccgcaagcct gctcggggcg
      541 ttagccaacc aagcgatttc acggctgtcc cgggtggcac acaggttgcg gctggacgtc
      601 gggatgaaga acgaggagaa gccgccggct gtcggcgacc tgccgccttc cggcaacggc
      661 catggcgcgt cggcgttcga cgtgcgtggt gtggcttcgg cgccggaggc ggcggccgcc
      721 cgaagcgacc ggcttcgccg ctggcttccc ctcggcgtcc tcgcgctcgc cgcgctcctg
      781 ctcgttgcgc tgctggcccc gcgactcgtc tcgtcgctcc ggtcgccggc gccggcgggt
      841 ctgctcttcg ccagcggtcg gatcgaaggc cggatcacga cgctcacgcc gaagtcgtcc
      901 gctcgcgtga ccacgctgca catcgacgag ggacagccgg ttcgcgcggg cgatctgctg
      961 gcgacgctcg acgaccgggc ccagcgggag cgcgtgaacg cggcccagca gtacctcgac
     1021 gtgctggtcg agcggctgcg ggcggccgac acggagctcg ccatgaccga gcgacaggta
     1081 tcgctgcaga tcgagcaggc cgaagcggcg ctgcgcgagg cggacgcgcg cctgcagcgc
     1141 gcccgtacga gcgagctgca ggcgaggcgt gacgcccagc gcgccgccat cctggtggcg
     1201 aaggaactca tcgccgctca ggtggccgag cacgcgcggc tccaggcgga cctcgaggtc
     1261 gaggccgtcc gggaggcgga ggaagcgctc gcgcgcacgc gaaagcagct ggagcaggcg
     1321 cgcctggggc cgcagcaggt ggcggccagg cgggccgagc gcgacgcgct cgagcgtcag
     1381 cggcggcagg cgcaggccca gctcgccgag caggcgagcg tcgtgaccga cttcgcgatc
     1441 cgcagcccga tcgccggccg ggtgctcacg cgcaccatcg aggtcggcga gcgcgtggag
     1501 gccggcacgc cgctcttcac cctggtggac ctcgaccggc tctacgtgaa gatctacgtg
     1561 cccgagccga gcatcggcaa ggtggccctc gggcaggagg cgcgcgtgta cgtggacgcg
     1621 catcccggcc ggcccttcgc cgcgcgggtg agcaaggtgg cgcaagaggc ggagttcacg
     1681 ccgaagaacg tcgagacgcg ggaggagcgc gtcaagctcg tcttcgccgt cgaggtcgcc
     1741 ctcgcggaga atcccggcgg cgtcctcaag cccggcatgc cggccgacgc cgtgatccgc
     1801 tggcagccgg acgcggcctg ggtgacgccc gccgacgcct cgcgccgcgg cgtcttacgg
     1861 cgagagggcg cgacgaggcc gcccggcgag accaggtagc gtccgcggcg gcgatgcagc
     1921 gcctcgacgg ggaagcggcg gcgatcgacg cgcgcagact cagcaagacc taccgccgcg
     1981 ggcgcgtcca cgcggtgcgg gacgtgtccc tccgggtggc cgccggcgag gtcttcggtc
     2041 tcatcggccc cgatggcgcg gggaagacga gcgtgctgca gatcctcgcc ggtgtgcttt
     2101 ccatcgacgg cggcgaagcc cgcgtggcgg gcgtggacgt cgcgcgcgac cccgaacggg
     2161 tgaaggcgct catcggctac atgccgcaag gcctcggcct caacctctac gacgcgctca
     2221 cggtcgagga gaacatcgag ttttttcgcg ctctccgccg cgtgccggcc gatcggtacc
     2281 gggacaaccg cgaccgcctg ctccggatga cgcggctcgc cccgttcctc gaccgacccg
     2341 cccggaagct gtcgggcggg atgcggcaga agctcgcgct catctgcacc ctcattcatc
     2401 tgcccgacgt cctgctgctc gatgagccga ccaccggtgt cgatcccctc tcgcgtcgtg
     2461 acttctggac catcatccac acgctggtcg ccacccgcgg cgtcaccgtg ttgctgacca
     2521 cgtcgtacat ggacgaggcc gagcgctgcc atcgtgtcag cctcatgcac gagggcgctc
     2581 tgatcgcggg cggtaccccg gcggcgctcg cggcgtcgct ggcggggccg gtcccgggca
     2641 cgctcgagga cgtcttcgtc caccacctga ccgcggggcg gccgctgccc gacgtcggct
     2701 cccgcctcgc gccggcccgg gagggggccg catcgaccgg gaacgggggg cgcgccgccg
     2761 gcggcgcgcc ggtcgtcgcg aacgggctca cctgccgctt cggcgcgttc acggcggtgg
     2821 acgcggtgag tctcgaggtc caccaggcag agatcgtggg gctcctcggg cccaacggcg
     2881 ccggcaagac caccctcatc aagatgctgt gcgggctgca gccgccgacg gcgggtagtg
     2941 ccagcgtggc cggctacgac gtcggccggc agggacgcga cgtgcgccga agcatcggct
     3001 acatgtcgca gcggttctcg ctctaccgcg accagaccgt tctcgagaac ctcgggctgt
     3061 ccgccgggct gtacggcatt cgcggaagcc gagggcggag ccgcatcgag gccctgctgg
     3121 cgagcctggg gctcgcgccg ttcgcggacc gattgccggc ggcccttccc ctcggcctcc
     3181 gccagcggct ggccctggcc tgcgccatcc tccacgagcc gcgcgtcctg ttcctcgacg
     3241 agccgacggc cggcgtcgat ccgctcgctc gccgggactt ctggaacctc gtgcaccagc
     3301 tggcgcacga ggacggtgtc gcggtcctcg tctccacgca ttacatggac gaggtcaccc
     3361 actgcgatcg tctgggcttc atgcacgagg gccggctggt gggtctcggc cggccggagg
     3421 agctggcgaa ggaggccgag cggcacggag ggccgatggt cgcggtggag acgccgacct
     3481 tcgaccgcgc cttcgtgctg ctccgcgatc agttcccggc cgccatgctc tacgggcgcc
     3541 gcatccgctg gcagagcgtc cagccggacc atgacatcca ccgggcacgg caggtgctcg
     3601 ccgcggccgg tatcgaggcc agcgtcggca cccagccgct ctcgatggag gacaccttcg
     3661 tcagcgtcct gcgcgtcgcg ggcctcagcc atgcttgacc gcaccctggc gatcgctcgc
     3721 aaggaggcga gggagctgct gagggatccc gcctacctcg ggctcgcgtt cgtcgtgcca
     3781 ctgctgctgc tgctcgtcct gggtcaccag ttgtccgtgg acgtcaaggg actgctcgtc
     3841 gcggtcgtgg accgggatcg gtcgccgtgg agccggcagt acgtcgacgg cttcgtgcat
     3901 tcggagtact tccggctggt cgggttcctc gacggcgagg cggaggcccg ggagtggatc
     3961 cggtcgggac gggcgcgcgt cgtcatcgac atcccgccgg acttcgggcg gcggctcagc
     4021 ggcggcgagc gcgccaccgt cggggtgacc gtcgacggct ccttcccgac gcgcgggcag
     4081 atcgcgctcg ggtacgtgga agcgatcggg gcgcttcaca acgaacgcct cgcccaggcg
     4141 ttcttcgccg agcgcggccg gcgcgacggc cccatctggc cggtccagat ggacgtgtcg
     4201 gtctggtaca accccacgct cgagagcaag aacttcatcg tgcccggcat gctggtggtg
     4261 atcctgatga tctttccacc gctcctgacc tcgctggtga tcgtccgcga gcgcgagagc
     4321 ggcaccatcc tgaacctcta ctgctcgcca gccagccgct gggacatcgt cgcgggcaag
     4381 gccctcccct acgcggccgt gtccttcgtc gactacctcg tgatcttcgc ggcgagcgtc
     4441 tggatcttcc aggtgcggtt cgtgggcagc gtgtggctgc tgtcggccgg cgcggtcctc
     4501 tactccatct gcaccgtggg gctcggcctg ctgatctccg ccttcacccg gacccagctc
     4561 gccgcggtgc tggcgacgtt cctggtcgcg gtcgcgccgg cgttcacgtt ttccggcatc
     4621 ttcgcgcccg tggccagtca ggatgccgtc gggcagggcg tgagctacct gatcccggcg
     4681 acatatttca tggacgtcgt ccggggcagt ttcctgaagg gcggcgggat cgccacccac
     4741 ggagcccggc tgctcgcgct cgccgcctac gcggtcgtgg tctacaccac cgcggccttc
     4801 ctcgtgcgca agcgggtggg ctgatggcga gccggctcgc ggcgatgatc cggaaggagt
     4861 tcagccagct gttccgcgac ctgcccatgc tcttgatcct cctctgggcc ttcaccggcg
     4921 ccgtctacac cggcggccac gggatgtcca cggagctcca gaaccatccg atcgccatcc
     4981 tcgacctgtc gcggagcgcg gagagccgcg agctcgtctc gcgcttccgc gccccgcact
     5041 tccgggtcgc ggcctacgcc gccagcgatg cgcagctcgt cgctctgctc gatgcgggcc
     5101 gggtgagcct cggcatcgtc atcccgcccg acttcgcgcg ccaggcccac gacgccggcg
     5161 cgcgctttca ggtcatcgtg gacggcagcc agtcgacgct ggccacgatc gccggggccc
     5221 acatcgcgag catcgccagc ggctacggcg tcgacttcct cgagggccgc tccggcggcc
     5281 gcgtcgggga gggcctgccg cgagtcgacg cacgcatccg ggtcgagtac aaccccaacc
     5341 ttcaagagcg gtggttttcg agcgtgctcg aggtgctgaa ccaggtcacc atgatcgcga
     5401 tgctgctagc ggcggcggcg atggtgcggg agcgcgagca gggcaccttg gaatacctgc
     5461 tcgtgagccc gctccgtccc gtcgagctct tcgccacgaa ggccgttccg gtcgtgacgc
     5521 tggtgcccct cgccgcgctc ggagcgctct tcggaatcgt tcagggtgtc ttcggtaccc
     5581 cgatccgggg cagcgtggtc ctgttctatg ccgtctcggt ggtctacgtc ttcgccatgg
     5641 ccagcctggg tctcgccatc gccgtggtgg ccaggaccct ggggcaggcg gttctgctcc
     5701 tcctgctggt cttctacccc atggtgctcc tctccggcgc gttcacgccc ccggagagca
     5761 tgggcgcggc catgcggtac gcgggcctgc tgtcgccggc gcgccactac gtcgacttcg
     5821 gctaccaggt cctcttcaag ggcaacggtc tcgcgtacgt ctggccgcag gtcgccggca
     5881 ttctcgcgct cgggctcgtc gtgttcgcga tctcggtgcg acgcttcgcg cgtctcgtgc
     5941 gctgacgcgt cggcaagtct ccgatgtcgg ggaggggccg cctcacgtgg agcgcggcgg
     6001 cggcagccat cgcggccatg cgattgaaat cgcgtgatga acgtctggca tcgacgatgc
     6061 accgacctgc gagagcgtgc cggatccgtc ccggcgcacg aagggggtgc ggggctggga
     6121 gcgccgggac cgtcgaggga ggagttgctg gcgcgggccg cgcagcccgg cgaggaggcg
     6181 ttgcggctcc acgcgctctg cggcggcaag atgcagaccc tcccgaagtg cc
//
LOCUS       DATIAZ010000286         7396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_739934, whole genome shotgun sequence.
ACCESSION   DATIAZ010000286 DATIAZ010000000
VERSION     DATIAZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739934"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VLF19_10040"
     CDS             complement(<1..214)
                     /locus_tag="VLF19_10040"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HSE93635.1"
                     /translation="MELLVGTLLLRPYVFAFLTAFLIAGAADVGARRALGFAGVVWPL
                     AWLAEFASTRWGLPFGLYHYTGATRGR"
     gene            254..2158
                     /gene="shc"
                     /locus_tag="VLF19_10045"
     CDS             254..2158
                     /gene="shc"
                     /locus_tag="VLF19_10045"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSE93636.1"
                     /translation="MTGLHDAIARAQAHLFSRQAPDGHWIGEVEADSTITSEHLLLCH
                     LVDRVNAEHERKAVRYLRRRQCADGGFNLFEGGPTNLSATIKAYFAMKLAGVPLGDPV
                     MAKARERILEMGGPSKANVFTKIQLALFGEYDWNGVPAMPVEIMLLPPPFFLFNIYEV
                     SYWSRTVIVPLLIIMDRKPVKWLPPERSLDELWPVPRERTSLRFPRMPRPFAWRDLFW
                     KNVFIAADDGLKIWERFSPRPLRKRAVEAARVWLEEHLAVPGGLGGIYPAMANSILAM
                     RLLGYADDHPLVAGQLKEIEALAVEAADEVHYQPCVSPVWDTGLAVNALIESGVAPDH
                     PALRRAGEWMVDRQVFTPGDWAVKRPYVQPGGWAFQYDNAFYPDLDDAAVVLMALEKL
                     RGLDADRVRLAKERGLGWCLGMQGQDGGWASFDADNNRLYLNNIPFADHGALLDPSTE
                     DLTGRGLELLGTFGYGPDFEPARRALGFIRRAQRLDGPWFGRWGVNYIYGTWSVMRGL
                     AAIGEDLRHESVQRAVRWLERRQNPDGGWGETCESYDRPELAGRGPSTPSQTAWALLA
                     LLAAGRAVTPVVERGVAHLLAAQKPDGGWDDAYWNGTGFPRVFMLKYHLYAKYFPLWA
                     LGVYRRALDG"
     gene            2151..2945
                     /locus_tag="VLF19_10050"
     CDS             2151..2945
                     /locus_tag="VLF19_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HSE93637.1"
                     /translation="MADGSTPILLRPLEALGERVLGVLEALGRFGSFFGIALAYLVMP
                     PYKLRPFIDRIHYIGFRSLSIILLTGAFTGMVLALQLFLTLTRFGSEAFLGPAVALSL
                     VRELGPVLAALMVTGRAGSALTAEIGIMRITEQIDALTVMALSPFRYLVTPSILAGLV
                     TFPLMTALFDLVGILGGYLVGVGLFGVGEGTYFGEMQAFVGMDDIMTGFWKSLSFGVI
                     VTWVCTYKGYYVARGAEGVAHATTQAVVLSSVLILLWDYFLGSVLP"
     gene            2986..3726
                     /locus_tag="VLF19_10055"
     CDS             2986..3726
                     /locus_tag="VLF19_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93638.1"
                     /translation="MIKVQDLRKSFRGQPVLRGVDLEVPDGSITVIIGRSGGGKSVFL
                     KHLLGLVRPDAGRVIVDDVDLTGLSGRAIDRVRERYGVVFQGGALFDSLTCAQNIAFP
                     LREKTRLRRAEIARRVATALTQVGLEGVDAKYPEEISGGMRKRVAIARALVTEPQIVF
                     FDEPTTGLDPILVNSIHRLIVDLHRRRRFTAVMVSHEIPEIFEIAETVAMLHEGRIVE
                     VGPPAAVRASANPVVQQFIRGEPDVGSA"
     gene            3739..4179
                     /gene="mlaD"
                     /locus_tag="VLF19_10060"
     CDS             3739..4179
                     /gene="mlaD"
                     /locus_tag="VLF19_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HSE93639.1"
                     /translation="MDRSRVNIAVGIFVVLGVLALGYLSIKLGRVAFFAGGGYSVVAD
                     FPSVGGLRVGASVEIAGVEIGRVEAIGLADYQARVTLRLNRDVELQEDSIASIKTKGL
                     IGEKFIRISPGASEKLIKPNGRIREVEAPVDLEELISKYVFGKV"
     gene            4203..4835
                     /locus_tag="VLF19_10065"
     CDS             4203..4835
                     /locus_tag="VLF19_10065"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93640.1"
                     /translation="MRCRSVVLLRTLALLVGLAVARPVAADEPLEQLKSRLDRAVRTL
                     EDPAFKTPSRAGERRAAVRRIAEDIFDFGETARRSLAHHWQARTPAEREEFVRLFADL
                     LERSYISKIALFNGERVTYVGETVDGDAAVVRTKIISRQGTGLPIDYRMLRRGGRWLV
                     YDVIIEGVSLVANYRSQFNKIIQTSSYQELVKKMKTKQEELLEQDRPGRT"
     gene            4832..6499
                     /locus_tag="VLF19_10070"
     CDS             4832..6499
                     /locus_tag="VLF19_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HSE93641.1"
                     /translation="MSKARALRELLARPGLVAAVGAHDALSARLIEAAGFPVVWSSSF
                     TVSAAHRAMPDVNLLTMTETLEAARHIDCAVGIPVIADCDNGYGNAVNVVRTVEEYER
                     VGIAGICFEDNLFPKKCSLYPGMRRELASIEEHAGKIRAATRARRDPATVVVARTEAL
                     IAGWGMTEALRRAEAYAGAGADMILVHSKAPTAAEVLEFMRLWDGRAPIVVVPTLYPG
                     VRWEELEGAGIKLVIWANQVLRGAVKGVQETLATLGRERRLDALAPHIVTLEEIYRHV
                     GVDDFKRIESEFLPRGVSDVRAVVIAAGSGKSLLPLTEDRPKCMLDIKGRTVLDRQIE
                     TLRAAGIQEIVVVRGYRKDVVTAAGVRFYDNDAYEETGELASLFAAGPELHGRVLFLY
                     SDVLFERALLDKLLRAEGDVVVAVDRAWVDQRDRLLPLVKPVDLVVTSDPPRPGLRSL
                     GEEQRDALVHVGQRVPPESATGEFTGLALFSPHGIVTARETWARARAADPAPFHEAES
                     AARADFTDLLQVLVAAGQPVTCVSTWKGWLDIDTFEDYQRAWAEIRG"
     gene            6502..>7396
                     /locus_tag="VLF19_10075"
     CDS             6502..>7396
                     /locus_tag="VLF19_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSE93642.1"
                     /translation="MTGAEFAALLTREGFDVSAGVPCSLVEPLIAALEGHPRLPWIET
                     VREDVAVGLCGGAWLGGRRPVVVMQNSGLGTSLNALASFSLMYGLPALLVVTWRGHGG
                     KDAPEHILTGAITEPLLELLGIPHRVLAAASAEADVRWARAEMDARLQPVALVVPPGL
                     LSAGASTTSPETSPGTVVAAAKPPLVQPTGAPGEPLVARISRLQAIEAALKEVGDAAV
                     VSANGYPSREAYAVTDRPQNFYMIGSMGLASSIALGVALARPARRAVVLDGDGNLLMN
                     LGVVANVASLAPRNYVHCVFDN"
BASE COUNT         1037 a         2566 c         2652 g         1141 t
ORIGIN      
        1 cgcggccgcg cgtggcgccc gtgtagtgat agaggccgaa gggcaggccc cagcgcgtcg
       61 acgcgaactc ggcgagccac gccagcggcc acacgacgcc ggcgaagccg agcgcccggc
      121 gcgcgcccac gtcggctgcg ccggcgatca ggaaggccgt caggaaggcg aagacgtacg
      181 ggcgcaggag tagcgtgccg acgagcagtt ccatggtttc gggcggcgag agcgcatgtt
      241 accatctggc ctcgtgacag ggctgcacga cgccatcgcc cgcgcacagg ctcatctctt
      301 ctcgcgccag gctcccgacg gtcactggat cggcgaggtc gaagccgaca gcaccatcac
      361 gtccgagcac ctgctgctct gccacctggt cgatcgggtg aacgccgagc acgagcggaa
      421 ggccgtccgc tacctccggc ggcggcagtg cgcggacggc ggattcaacc tgttcgaggg
      481 tgggccgacg aatctgtcgg cgaccatcaa ggcctatttc gcgatgaagc tggccggcgt
      541 gccgctgggc gacccggtga tggcgaaggc ccgcgagcgg atcctcgaaa tgggcgggcc
      601 cagcaaggcc aacgtgttca ccaagatcca gctcgcgctg ttcggcgagt acgactggaa
      661 cggcgtcccg gcgatgccgg tggagatcat gctcctgccg ccgccgttct tcctcttcaa
      721 catctacgag gtctcgtact ggtcgcggac cgtgatcgtc ccgctgctga tcatcatgga
      781 ccgcaagccc gtgaagtggt tgccgcccga gcggtcgctc gacgagctgt ggccggtgcc
      841 gcgcgagcgg acgagcctgc gcttcccccg catgccccgg ccgttcgcct ggcgtgacct
      901 cttctggaag aacgtcttca tcgccgcgga cgacgggctc aagatctggg agcgcttctc
      961 gccccggccc ctgcgcaagc gcgccgtcga ggccgcccgg gtgtggctcg aggagcatct
     1021 cgcggtgccg ggcgggctcg gcggcatcta cccggccatg gccaactcga tcctggccat
     1081 gaggctgctc ggatacgccg acgaccatcc gctcgtggcc ggccagctga aggagatcga
     1141 ggccctcgcc gtcgaagcgg ccgacgaggt ccactaccag ccgtgcgtct ctccggtctg
     1201 ggacacgggg ctcgccgtga acgcgctcat cgagagcggc gtcgcgccgg accaccccgc
     1261 gctccggcgc gccggcgagt ggatggtgga ccgacaggtc ttcacgcccg gtgactgggc
     1321 ggtgaagcgg ccctacgtgc agccgggcgg ctgggcgttc cagtacgaca acgccttcta
     1381 tccggacctg gacgacgcgg cggtcgtgct gatggcgctc gagaagctgc gtgggctcga
     1441 cgccgaccgc gtgcggctgg cgaaagagcg gggcctcggc tggtgcctcg gcatgcaggg
     1501 acaggacggc ggctgggcct cgttcgacgc cgacaacaac cgtctctacc tcaacaacat
     1561 ccccttcgcc gaccatggcg cgctcctcga cccgtccacg gaggacctca ccgggcgcgg
     1621 cctggagctg ctcggcacct tcggctacgg gccggacttc gagcccgcgc ggcgcgcgct
     1681 cggcttcatc cgccgcgcgc agcgcctcga cggcccgtgg ttcggacgct ggggtgtcaa
     1741 ctacatctac ggcacgtggt cggtgatgcg cggcctcgcg gccatcggcg aggacctgcg
     1801 ccacgagtcc gtgcagcgcg ccgtccgctg gctcgagcgg cgccagaacc ccgacggtgg
     1861 ctggggcgag acgtgcgaga gctacgaccg gccggagctg gccgggcgcg ggccgtcgac
     1921 gccgagccag acggcgtggg ccctgctcgc gctgctcgcc gccgggcgcg cggtgacgcc
     1981 ggtcgtcgag cgcggcgtcg cgcacctgct cgccgcgcag aagcccgacg gcggctggga
     2041 cgacgcctac tggaacggca ccggttttcc gcgcgtgttc atgctgaagt atcacctgta
     2101 cgcgaagtac ttccccctgt gggcgctcgg ggtctaccgc cgcgccctcg atggctgacg
     2161 gttcgacgcc gatcctgctg cggccgctcg aggcgctggg cgagcgggtg ctcggggttc
     2221 tcgaagcgct cggccgcttc ggatccttct tcggcatcgc cctggcgtac ctggtcatgc
     2281 cgccgtacaa gctgcgcccg ttcatcgacc ggatccacta catcggcttc cgctcgctga
     2341 gcataatttt actgaccggc gcgttcaccg gcatggtcct cgcgctgcag ctcttcctga
     2401 ccctgacgcg cttcggctcc gaggcgttcc tcgggccggc cgtggcgctc tcgctggtcc
     2461 gagagctggg gcccgtgctc gccgccctca tggtgaccgg acgcgcgggc tcggcgctca
     2521 cggccgagat cgggatcatg cggatcaccg agcagatcga tgcgctcacg gtgatggcgc
     2581 tgagcccgtt ccgctatctc gtcacgccgt ccatcctggc ggggctcgtc acgtttcccc
     2641 tcatgacggc gctcttcgac ctcgtcggca tcctcggcgg ctacctcgtg ggggtcgggc
     2701 tgttcggggt gggcgagggc acgtacttcg gcgagatgca ggcgttcgtg ggcatggacg
     2761 acatcatgac cggcttctgg aagtcgttgt ccttcggcgt gatcgtcacc tgggtgtgca
     2821 cctacaaggg ctactacgtc gcccgcggcg ccgagggcgt ggcgcacgcg acgacccagg
     2881 ccgtggtgct ctcctcggtg ctgatcctcc tctgggacta tttcctgggg tccgtcctcc
     2941 cgtgatcgtg gccgggcgag ccgcagtcgc agacgaggcg agcctgtgat caaggtccag
     3001 gacctgcgca agtcgttccg cggtcagccc gtgctccgcg gcgtggacct cgaagtgccc
     3061 gacggctcca tcaccgtgat catcgggcgc agcggcggcg ggaaatccgt gttcctcaag
     3121 cacctgctgg ggctggtgcg cccggacgcc gggcgggtga tcgtggacga cgtggatctc
     3181 accgggctct ccgggcgcgc catcgatcgg gtgcgcgagc gctacggcgt cgtcttccag
     3241 ggcggcgcgc tcttcgactc gctcacctgc gcccagaaca tcgccttccc cctgcgcgag
     3301 aagacccgcc tccggcgggc cgagatcgcc cgccgtgtcg ccaccgcgct cacgcaggtg
     3361 gggctggagg gggtggacgc caagtacccc gaggagatct cggggggcat gcgcaagcgc
     3421 gtggcgatcg cccgcgcgct cgtgaccgag ccccagatcg tgttcttcga cgagccgacg
     3481 acgggcctgg atccgatcct cgtcaacagc atccatcggc tcatcgtcga cctgcaccgg
     3541 cgccgccgct tcaccgcggt gatggtgagt cacgagatcc ccgagatctt cgagatcgcc
     3601 gagacggtgg ccatgctgca cgagggtcgc atcgtcgagg tgggcccgcc ggcggccgtc
     3661 cgggcctcgg cgaatcccgt cgtccagcag ttcatccgcg gcgagccgga cgtcggcagc
     3721 gcgtaggagg tcgagcctat ggaccgctcc agagtgaaca tcgccgtcgg catcttcgtc
     3781 gtcctcggcg tgttggcatt gggatacctt tccattaaac tcggcagggt cgcgttcttc
     3841 gccggcggcg ggtacagcgt cgtcgcggac ttcccatcgg tgggaggtct gcgcgtgggg
     3901 gcgagcgtcg agatcgccgg cgtcgagatc gggcgcgtgg aagcgatcgg gctggcggac
     3961 taccaggccc gcgtcaccct gcgcctgaac cgggacgtgg agctgcagga ggattcgatc
     4021 gcatccatca agaccaaggg gctgatcgga gagaagttca tccggatcag ccccggggcg
     4081 tccgagaagc tcatcaagcc caacggacgc atccgggagg tcgaggcgcc cgtcgacctg
     4141 gaggagttga tctcgaagta cgtcttcggc aaggtgtgag cgggcacacg gaggctggtg
     4201 gaatgaggtg ccgcagtgtc gtgctgctga gaaccctggc gctgctggtg ggtctggccg
     4261 tcgcccgccc ggtggcagcc gatgagcccc tggagcagct gaagagccgg ctcgaccggg
     4321 ccgtcaggac gctggaggac ccggcgttca agacgccgtc cagggccggg gaacgacggg
     4381 ccgccgttcg ccgcatcgcc gaggacatct tcgacttcgg ggagacggcc cggcggtcgc
     4441 tggcgcacca ctggcaggcc cggacgccgg cggagcgcga ggagttcgtg cggctcttcg
     4501 ccgatctgct cgagcgctcg tacatctcca agatcgcgct gttcaacggc gagcgcgtca
     4561 cctacgtcgg cgagaccgtc gacggcgacg cggcggtcgt ccggacgaag atcatcagcc
     4621 ggcagggcac gggactcccc atcgactacc ggatgctccg gcgaggcggc cgctggctcg
     4681 tctacgacgt catcatcgag ggcgtgagcc tcgtggccaa ctaccggagc cagttcaaca
     4741 agatcatcca gacctcgtcg taccaggagc tcgtcaagaa gatgaagacc aagcaggaag
     4801 agctgctcga gcaggaccgg ccgggtcgca cgtgagcaag gcgcgcgcgc tccgcgagct
     4861 gctggcccgc cccggcctgg tcgccgccgt cggcgcccac gacgcgctca gcgccaggct
     4921 catcgaggcc gccggcttcc cggtcgtgtg gtcgtcgagc ttcacggtgt cggcggccca
     4981 ccgcgccatg cccgacgtga atctcttgac gatgacggag acgctggagg cggcgcgcca
     5041 catcgactgc gccgtcggga ttcccgtcat cgccgattgc gacaacggtt acggcaacgc
     5101 cgtgaacgtc gtccggacgg tcgaggagta cgagcgggtg ggcatcgccg gcatctgctt
     5161 cgaggacaac ctcttcccca agaagtgcag cctctatccc gggatgcgcc gggagctggc
     5221 ctcgatcgag gagcacgccg gtaagattcg cgcggcgacg cgcgcccggc gcgacccggc
     5281 gaccgtcgtc gtcgcgcgca ccgaggcgct catcgccggc tggggcatga ccgaggcgct
     5341 gcgccgggcc gaggcctacg ccggggccgg ggccgacatg atcctcgtgc actccaaggc
     5401 gccgacggcc gccgaggtcc tggagttcat gcgcctctgg gacggccgcg cgccgatcgt
     5461 cgtcgtgccc acgctctacc cgggggtccg ctgggaggag ctggagggcg ccggcatcaa
     5521 gctcgtcatc tgggccaacc aggtgctgcg cggcgccgtc aagggcgtgc aggagacgct
     5581 cgcgacgctg ggccgcgagc ggcgcctcga cgcgctcgcc ccgcacatcg tgacgctcga
     5641 ggagatctac cggcacgtcg gcgtggacga cttcaagcgc atcgagagcg agttcctgcc
     5701 ccgcggggtc tccgacgtgc gcgccgtcgt catcgccgcc ggctccggga agtcgttgct
     5761 cccgctgacc gaggaccggc ccaagtgcat gctcgacatc aagggccgca ccgtgctcga
     5821 ccgccagatc gagacgctcc gggcggccgg catccaggag atcgtcgtgg tgcgcgggta
     5881 ccgcaaggac gtggtgaccg ccgcgggcgt gcggttctac gacaacgacg cctacgagga
     5941 gacgggcgag ctcgcgtcgc tcttcgcggc cgggcccgag ctgcacgggc gagtgctctt
     6001 cctttactcc gacgtgctgt tcgagcgcgc cctgctggac aagctgctgc gcgccgaggg
     6061 cgacgtcgtc gtcgccgtgg accgcgcctg ggtggatcag cgcgaccggc tcctgccgct
     6121 cgtcaagccc gtggacctcg tggtcacgtc cgacccgcca cggccggggc tccgctcgct
     6181 gggcgaggag cagcgcgacg ccctcgtgca cgtggggcag cgcgtgccgc cggagtcggc
     6241 gacgggcgag ttcaccgggc tcgcgctctt ctcgcctcac ggcatcgtga cagcgcgcga
     6301 gacgtgggcc cgcgcgcggg ccgccgaccc cgcgccgttt cacgaggccg agagcgcggc
     6361 gcgggcggac ttcacggacc tgttgcaggt gctggtggcc gccggccagc cggtgacgtg
     6421 cgtgagcacg tggaagggct ggctggacat cgacacgttc gaggactacc agcgcgcctg
     6481 ggccgagatc agagggtaag cgtgaccggc gccgagttcg ccgcgctgct cacgcgcgag
     6541 ggcttcgacg tctctgccgg cgtgccctgc tcgctggtcg agcccctgat cgccgcgctc
     6601 gagggtcatc cccgcctgcc ctggatcgag accgtccgcg aagacgtcgc cgtcggcctc
     6661 tgcggcggcg cgtggctggg cgggcggcgg cccgtcgtcg tgatgcagaa ctccgggctc
     6721 ggcaccagcc tcaacgcgct ggcctccttc tcgctcatgt acggtctgcc ggcgctgctg
     6781 gtggtgacgt ggcgcggcca cggcggcaag gacgcgccgg agcacatcct gaccggcgcc
     6841 atcaccgagc cgctgctcga gctgctcggc atcccccatc gcgtgcttgc cgccgcctcc
     6901 gccgaggcgg acgtccggtg ggcccgcgcg gagatggacg cgcggctgca gcccgtcgcg
     6961 ctcgtcgtac cgcccgggct cctgtcggcg ggggcctcga cgacctcccc cgagacgtcc
     7021 cccgggacgg tggtcgcagc ggcaaagcca ccgctcgtgc agccgaccgg ggcgccgggg
     7081 gagccgctgg tggcgaggat ctcgaggctt caggcgatcg aggcggcgct gaaggaggtg
     7141 ggggacgcgg cggtggtgtc ggcgaacggg tatccgtcgc gcgaggcgta cgcggtcacc
     7201 gaccggccgc agaacttcta catgatcggc tccatgggtc tcgcctcgtc gatcgcgctg
     7261 ggcgtggctc tggcgcggcc ggcgcgccgc gcggtcgtgc tcgacggcga cgggaacctc
     7321 ctgatgaacc tcggcgtcgt cgccaacgtg gcgtcgctgg cgccccgcaa ctatgtgcac
     7381 tgcgtcttcg acaacg
//
LOCUS       DATIAZ010000287         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_610089, whole genome shotgun sequence.
ACCESSION   DATIAZ010000287 DATIAZ010000000
VERSION     DATIAZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610089"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VLF19_10080"
     CDS             <1..1488
                     /locus_tag="VLF19_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HSE93643.1"
                     /translation="HLRFDDTNPTKESQEYVDAIVRDIRWLGFDWGRHLYHASDYFEQ
                     LYDWAVQLIRDGKAYVDDLTAEEMRETRGTLTEPGRPSPWRDRSVAENLELFARMRRG
                     EFEDGSRTLRAKIDMASPNLNLRDPVMYRIRKTSHQRTGDAWCIYPMYDWAHGQSDSI
                     EGVTHSLCSLEYEDHRPLYDWYLDALGIYHPRQIEFARLNLSHTTLSKRKLLTFVEEG
                     IVSGWDDPRMPTLAGLRRRGYTPESIRDFAERIGVAKADNLVDVALLEHCVREDLNKR
                     AQRRMAVLRPLKLVIENYPEGQVEELEAINNPEDPSAGTRNVPFGRILYIEQDDFRDP
                     APPKYFRLSPGALVRLRYAYIVECTDVVRDAAGAVVEVRCKYDPSSRGGDSKGRKVKG
                     TIHWVSAAHAVDAEVRLHDALFTVPRPDEADDFKTVVNPQSLEVVTGAKLEPSLAGVA
                     PESRFQFERVGYFCADWRDHSPARRVFNRTVVLRDTWAKIEKTGG"
     gene            1495..1860
                     /locus_tag="VLF19_10085"
     CDS             1495..1860
                     /locus_tag="VLF19_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93644.1"
                     /translation="MLEVKRGRAEPIDKQGTMIRFKVPLAGRPSEDWKVAFRQCLDTD
                     VAFFDARVTLHADHVTFQTEEENVGSGIKKIDDAIAVANSTAAQVEERKKLAKQVGRQ
                     AEVDKRQELERLKDKFKNL"
     gene            2082..2213
                     /locus_tag="VLF19_10090"
     CDS             2082..2213
                     /locus_tag="VLF19_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93645.1"
                     /translation="MDWSERVSVERVALRQPSALVERDERGGFPLRALLPTTVCGAR"
BASE COUNT          383 a          780 c          781 g          331 t
ORIGIN      
        1 cacctgcgct tcgacgacac caaccccacc aaggagagcc aggagtacgt cgacgccatc
       61 gtgcgcgaca tccgctggct cggcttcgac tggggccggc acctctacca tgcgagcgac
      121 tacttcgagc agctctacga ctgggccgtg cagctgatca gggacggcaa ggcctacgtc
      181 gacgacctca ccgccgagga gatgcgcgag acgcgcggca cgctgaccga gcccgggcgg
      241 cccagcccct ggcgcgatcg gagcgtcgcg gagaatctcg agctcttcgc gcgcatgcgc
      301 cggggcgagt tcgaggacgg cagccgtacg ctgcgcgcga agatcgacat ggcctcgccc
      361 aacctgaacc tgcgcgatcc tgtcatgtac cggatcagga agacctcgca ccagcgcacg
      421 ggcgacgcat ggtgcatcta tccgatgtac gactgggccc acggccagtc cgactccatc
      481 gagggcgtga cccactcgct gtgctcgctg gagtacgagg accaccgccc gctctacgac
      541 tggtacctgg acgcgctcgg catctatcac ccgcgccaga tcgagttcgc gcgcctgaac
      601 ctgtcgcaca ccacattgag caagcggaag ctgctcacgt tcgtggagga ggggatcgtc
      661 tcgggctggg acgacccgcg catgccgacc ctcgccgggc tgcgccggcg cggttacacg
      721 cccgagtcca tccgcgactt cgccgagcgc atcggcgtcg ccaaggccga caacctggtg
      781 gacgtggccc tgctcgagca ctgcgtgcgc gaggacctga acaagcgcgc gcagcgccgc
      841 atggccgtgc tgcggccctt gaagctggtg atcgagaact atcccgaggg ccaggtggag
      901 gagctggagg ccatcaacaa ccccgaggac ccgtcggccg gcacccgcaa cgtgcccttc
      961 ggccgcatcc tttatataga gcaggacgac ttccgcgacc ccgcgccgcc caagtacttc
     1021 cggctgtcgc cgggcgccct ggtgcgtctg cgctacgcct acatcgtcga gtgcacggac
     1081 gtggtgcgcg acgccgccgg cgccgtcgtc gaagtgcgct gcaagtacga cccttcgtcg
     1141 cgcggcggtg actcgaaggg gcgcaaggtg aagggcacca tccactgggt ctcggccgcc
     1201 cacgcagtgg acgccgaggt gcgcctccat gatgcgctct tcaccgtgcc caggccggac
     1261 gaggcggacg acttcaagac cgtggtgaac ccgcagtcgc tggaggtcgt gaccggcgcg
     1321 aagctcgagc cgtcgctggc gggtgtggcg ccggaatcgc gcttccagtt cgagcgtgtg
     1381 gggtatttct gcgctgactg gcgggatcac tcacccgcgc gccgggtgtt caaccggacg
     1441 gtggtgctgc gggatacatg ggcgaagatc gagaagacgg gagggtagcc gacgatgctc
     1501 gaggtcaagc gtggccgtgc cgaacccatc gacaagcagg gcaccatgat ccggttcaaa
     1561 gtgcccctgg ccggccgccc gtcggaggac tggaaagtcg cgttccgcca gtgtctcgac
     1621 acggacgtcg cgttcttcga cgcgcgcgta acgcttcacg ccgaccacgt gaccttccag
     1681 accgaagagg agaacgtggg atcgggcatc aagaagatcg acgacgcgat cgccgtcgcg
     1741 aacagcacgg ccgcccaggt cgaggagcgg aagaagctgg ccaagcaggt cgggcgccag
     1801 gccgaggtcg acaagcgcca ggagctggag cgcctgaagg acaagttcaa gaatctgtga
     1861 cggcggcccg tggccgcacc ctggcgctcg cccctccgcg gcatgcgtcc cgacggcggc
     1921 gccggtccac ggatacgggg atgtgcgtct ctcggggtct cgcgcgatac ggatctcaag
     1981 gccgccgcct ccgcggcacg ggcagggctc tcgcgggtct ccgtcgccga cggcaagcgc
     2041 cgggtgctga gcgccctgcg cctgaggcac gccgcctgga tatggactgg tccgagcgcg
     2101 tgagcgtcga gcgggtcgcg ctgcgacagc cgtcggcgct ggtggagcgc gacgagcgcg
     2161 gcggctttcc cctgcgtgcg ctgctcccaa cgacggtctg cggcgcgcgc tgacgcgcca
     2221 ctccaacgct gcccggcaga gtccgcttga ctctcctccc cggaggaggc tttac
//
LOCUS       DATIAZ010000288         9154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_213192, whole genome shotgun sequence.
ACCESSION   DATIAZ010000288 DATIAZ010000000
VERSION     DATIAZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213192"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..2612)
                     /locus_tag="VLF19_10095"
     CDS             complement(246..2612)
                     /locus_tag="VLF19_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HSE93646.1"
                     /translation="MRRPPLAVADRYLLRELAAPFGLGVALFTFFFVIDSLYELTELV
                     ITRGVPLVLVIQLVNFMLPALLSHTLPMALLVAVLLAGGRLAGDLEIVAFKAAGVSPL
                     RLLRPVMLAALLITVTTTSLTLVVNPIANQQFQAQLFKILQSRVASALKERVFNTSLG
                     DLVLYVEEISPSQVGIRGVLVSDERDPTLSRIVTAREGRIITDEANHRITLRLIDGGV
                     NEADVSPADPPPGVVTHGSPTVGGAASAKRYRYTKFAVYDMVLNLESSLKSAASLDRP
                     ERDLRFRDLRRQLAEARPGGDDRRRLEIELHKRFAFPAAALVFALLGFPLAVRSHRGG
                     RGVALVGTLVIVATYYLVLSSLEGVAQRGRLPVWLAVWAPNLGFGTVGGVLLLVATRE
                     WHGRNMRGLWRVVDAVWQRLPRPRRRREERATAARDTTLIIDRYLLRRYVGFVAIGMA
                     VATTLIVVGYFLQTLDRYLRVKPPLRYIAEHLLYVVPVNLYEAVPIVMLVATVFLFLT
                     LSRWHELTALKAAGISLYRVSAPVLAFGLVLTLAAGFFQEFVLPDLRERGDEVERVKI
                     RGEPPRHLRSRTRLWLRSSDTRFYRVELLSPVTADLYGVTILEIDRDFRLLNRVDARR
                     AHWTPVGWELSEGAFREIDRDGTVTTLSFDRTGLELSETIHEFTAIHKRPTAMNYREL
                     REYLARLEATGFQVQRYLVEMYAKLSNPLRSLIMILLAIPFALQSPRGGRLYGIALAI
                     GLMAAYVVMDYSARAFARADLLPPLLAAWTANVVFFGVGATMFLRART"
     gene            complement(2653..4848)
                     /locus_tag="VLF19_10100"
     CDS             complement(2653..4848)
                     /locus_tag="VLF19_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSE93647.1"
                     /translation="MATIVTADDKPRRARKKRETRRWVREVKAIALLAVAGFAFVALA
                     WFDPGLPAAEQRGPAGPVGVWLGWALFRSLGYAGFLVPLLLAVVAVSAFLRPLVGRGG
                     APVIGIAMLVLSATGLLAESANTFTGPEARDVVMRGGIAGYWLVEGLQATVGGAGTWL
                     VLLAAVPVGVLCVTQVSYGAASRLVSARLARWRKRRAALARPPRSAGGTSGIPGDEAL
                     PTDAVIAVEEAVPPPVVVESRKRVPSTALAWQETFDFGTGGAAAFQLPPVGLLQVPGA
                     SEHKITHEELHDNAETLRRKLQDFEVDGRIVQVSPGPIITSYEFEPAAGVKISRVVNL
                     ADDLALALKAASVRIVGPIPGRGTVAVEVPNAESAMVYLREIFASPDFADPRRRLPLA
                     LGKDVTGSAVVSDLTAMPHLLVAGATGTGKSVGLNAMICSILYKTTPAEVRFLMIDPK
                     RLELSVYEGIPHLLAPVVTDAKEAAARLRWIVGKMDERYKQLQLKQVRSIEGYNKVVE
                     PAERLPYWVVVIDELADLMLISAGDVQTSLVRLAQLARAIGIHLIVATQRPSVDVVTG
                     LIKANFPTRIAFQMASKVDSRTVLDANGAETLLGRGDMIFVPPGANKLMRVHGAWVAD
                     EEVKAVCEFLRKQGTAVYEEVVLPAEDAAGEGGDAAERDDLYWDCVRLVIGSRQASIS
                     FLQRRMGLGYPKAARFIDMMEQDRIIGPGQGAKPREILQGPEFLAKLGK"
     gene            complement(4848..6518)
                     /locus_tag="VLF19_10105"
     CDS             complement(4848..6518)
                     /locus_tag="VLF19_10105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HSE93648.1"
                     /translation="MEPLTEPAVRLIPLGGLGEIGLNMMLVEFGDDLVAVDCGLMFPD
                     PDEMPGIDHVIPDFAYALGKRDGFRAVFLTHGHEDHVGALPYLLRQVRVPVYGTPMTL
                     ALVAERLREHGLLEIADLRTMRPRDAIEAGPFRVEPIRVTHSIVDGIGLAIDTPVGTI
                     VHTGDFKLDPSPLDGEPPDFRRFDELGEQGVLLLCSDSTNVDRPGHTRPEIEVGQALR
                     ERFRQARGRVILATFASHIHRIQQVLTLAAEHGRSVALLGMSMQKNVAIASELGYLRV
                     PPGVLLALEDLVALPSSRQVILSTGSQGEPNSALALMAAGEHKAVQVERGDLVIISAR
                     VIPGHERTIGRVVNALLRLGAEVLWEDNAFVHVSGHASQEDLKRMLTLTRPRYFVPVH
                     GEYRHLLGHARLAEGAGLPADHVFLIEDGLGLELTKTAARVVGRFPVGRVLVDGKGVG
                     DVGAVVLRDRQVLAEEGIISVSLVVDRRGALVAGPEIASRGVVYVKENEVLLEELRGA
                     VRAVLAARDASAPADREGLGALVRAAVRQFINQRFHRKPVVLPVILEV"
     gene            complement(6625..7869)
                     /locus_tag="VLF19_10110"
     CDS             complement(6625..7869)
                     /locus_tag="VLF19_10110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSE93649.1"
                     /translation="MRWMPSRFRLVVLASFLVAGLAAAALAQRTAPPPPPVPVVATEP
                     PEPARAAAVDRLEKVSLKRGDTLVTALRRAGVPSRPAHEVAAALKKAGADLRRLRPGD
                     DLEIAWSPADQPTAVTWQASPWRSYAAVADEDGWSVKRLETAPDVRVQTVQGIVVRSL
                     FEAIDVAGETPPLVLALVAIFESDFDFTADTREGDRFRLLVEKRYAGETFVNYGRVLA
                     AQYESGGRRLTGIGFAQPGAKFAFYDLGGRAMRKSFLKSPLEFSRITSGFTYARPHPI
                     LGGVRPHLAIDYGAPVGTPVRAVADGAVVRAGWNGGNGIQVHLRHRSGYETLYNHLSR
                     VGAGIRRGARVTQRQVIGYVGATGLATGPHLDYRVAKGGVFVNPMSEKFIPGEPIPAA
                     GKAEFSRYAKSLLSRLEDEAPF"
     gene            complement(7866..8648)
                     /gene="rsmA"
                     /locus_tag="VLF19_10115"
     CDS             complement(7866..8648)
                     /gene="rsmA"
                     /locus_tag="VLF19_10115"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSE93650.1"
                     /translation="MSRRRALGQHFLRDAGIARAIVDLVAPAERDVVVEIGPGEGALT
                     AELARHPARITALEVDRALVPRLRERFPGVEVLEADAREWDYTTLRAEPGGRVLVVGN
                     LPYSVGTRILLALVQAHGAIAEMALMLQREVAERVAAAPGGKAYASLSILCQLHWDVR
                     VALRVPPGAFRPPPEVESAVLHLRALAGPRVALADERRFEAVVRAAFAQRRKMLANAL
                     SGGLGIPAESAREAAEAAGIDPRRRAETLSISEFASLAGRLS"
     gene            complement(8645..>9154)
                     /locus_tag="VLF19_10120"
     CDS             complement(8645..>9154)
                     /locus_tag="VLF19_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93651.1"
                     /translation="LLAEAGYPKGFDAGDLWCDAATATMSEATAGFLQAVGIRARVRP
                     LERAAFFRGYQEKKLKNLLYSISGAFGNAATRLEVFVAAGGPYVYGSYPDIDGLFREQ
                     AGELDRKRREAMLHRIQNLVHDKAMYAPIWELGFIHAQGPRVAESGLGLITGWAFSAP
                     YEDVKLKGK"
BASE COUNT         1373 a         3274 c         3317 g         1190 t
ORIGIN      
        1 gcgacgatgt ttctccgggc ccggacctag caccgcgaat cagcggttcg gtggtgagcg
       61 caccggccgg ggtggggtcg ggggcgcggg tccgccgacc tgttccccgc ccacggaacc
      121 ccgccccacc tgaagatgga aatagcaccc gagcgacgac gcgctgcgca tcaggggtcg
      181 gcggacccgc gcccccgacc ccaccccggc cggcgcgctc accaccggac cgctgattcg
      241 cgagactagg tccgggcccg gagaaacatc gtcgcgccca cgccgaagaa cacgacgttg
      301 gccgtccagg cggcgagcag gggaggcagc aagtccgccc gcgcgaacgc ccgcgcggag
      361 tagtccatga ccacgtaggc cgccatcagg ccgatggcca gggcgatgcc gtagaggcgc
      421 ccaccgcgcg gcgactgcaa cgcgaacggg atcgccagga ggatcatgat gaggctgcgc
      481 agagggttcg acagcttggc gtacatctcg accaggtatc gctggacctg gaagccggtc
      541 gcctcgaggc gggccaggta ctcgcggagc tcgcggtagt tcatggccgt gggccgtttg
      601 tggatcgcgg tgaactcgtg gatggtctcc gacagctcga gccccgtccg atcgaaggac
      661 agggtggtga cggtgccgtc ccggtcgatc tcgcggaagg cgccctcgct cagctcccag
      721 cccacgggcg tccagtgcgc ccgtcgggca tccacacggt tcaggaggcg gaagtcccgg
      781 tcgatctcga ggatcgtgac gccgtacaga tcggcggtga ccggactcag cagctccacg
      841 cgatagaagc gggtgtcgga gctgcgcagc cacaggcggg tccgactccg caggtggcgc
      901 ggcggctcgc cgcgtatctt cacgcgctcc acctcgtcgc cccgctcgcg caggtcgggc
      961 aggacgaact cctggaagaa tcccgccgcc agcgtgagca ccagcccgaa ggccaggacc
     1021 ggcgcgctga cccggtacag gctgatcccc gccgccttga gggccgtcag ctcgtgccag
     1081 cggctgaggg tgaggaacag gaacacggtg gccaccagca tcacgatggg cacggcctcg
     1141 tagaggttga cgggcaccac gtagagcagg tgctcggcga tgtaccgcag cggaggcttg
     1201 acccgcaggt agcggtcgag ggtctgcaag aagtagccga cgacgatgag cgtggtggcg
     1261 accgccatcc cgatggcgac gaaaccgacg tagcgacgca ggaggtagcg atcgatgatc
     1321 agcgtcgtgt cgcgggcggc tgtggcgcgc tcctcgcgcc ggcggcgcgg tcgaggcagc
     1381 cgctgccaga cggcgtccac gacccgccac agaccgcgca tgttccggcc gtgccactcc
     1441 cgcgtggcga cgaggagcag gaccccgccc accgtgccga agccgaggtt cggcgcccag
     1501 acggccagcc agaccggcag gcgtccgcgc tgggcgacgc cctcgagcga cgacagcacc
     1561 agatagtagg tcgccacgat cacgagcgtg cccaccagcg ccacgccgcg cccgccgcgg
     1621 tgggagcgca cggcgagcgg gaagccgagg agcgcgaaga ccagcgccgc cgccggaaac
     1681 gcgaaccgct tgtgcagctc gatctcgagg cggcggcggt cgtcgccgcc ggggcgggcc
     1741 tccgcgagct gccggcgcag atcccggaat cgcaggtccc gctccggcct gtcgaggctg
     1801 gccgccgact tcagcgagga ctcgagattg aggaccatgt cgtagaccgc gaacttggtg
     1861 tagcggtagc gcttggccga cgcggcgccc cccaccgtcg ggctcccatg cgtcacgacg
     1921 cccgggggcg gatcggccgg gctgacgtcg gcctcgttga ccccgccgtc gatcagccgc
     1981 agcgtgatgc gatgattggc ctcgtcggtg atgatccggc cctcgcgggc ggtgacgatg
     2041 cggctgagcg tcgggtcccg ctcgtcggac acgagcacac cccggatccc gacctgggat
     2101 gggctgatct cctcgacgta gaggacgaga tcgcccagcg acgtgttgaa gacccgctcc
     2161 ttcagcgcgc tggccacgcg cgactggagg atcttgaaga gctgcgcctg gaactgctgg
     2221 ttggcgatgg ggttcacgac cagggtcagg ctcgtggtcg tcaccgtgat cagcagggcg
     2281 gcgagcatca ccggcctcag gagcctcagc gggctgacgc cggcggcctt gaacgcgacg
     2341 atctccaggt cgcccgccag gcgcccgccg gccagcagga cggccaccag cagggccatc
     2401 ggcagggtgt gcgagagcag ggccggcagc atgaaattga cgagctggat gaccagcacg
     2461 agcggcacgc cccgggtgat gacgagctcg gtgagctcgt agaggctgtc gatgacgaag
     2521 aagaaggtga acagggccac cccgagcccg aacggcgccg ccagctcccg gagcaggtag
     2581 cgatcggcga cggcgagtgg gggtcgtctc atcggtctcg agtccccgtc cctgccggtc
     2641 gcgtcggcgc gcctacttcc ccagcttcgc caggaactcc ggcccctgga ggatctctcg
     2701 gggcttggcg ccctgccccg gaccgatgat ccggtcctgc tccatcatgt cgatgaagcg
     2761 cgcggccttg gggtagccga gacccatgcg gcgctgcagg aacgagatcg aggcctggcg
     2821 ggagccgatg accagtcgga cacagtccca gtagaggtcg tcgcgctccg ccgcgtcgcc
     2881 gccctccccc gccgcgtcct ccgccggcag cacgacctcc tcgtagacgg ccgtgccctg
     2941 cttgcggagg aactcgcaga ccgccttgac ctcctcgtcg gcgacccagg ctccgtgcac
     3001 gcgcatcagc ttgttcgctc cgggcggcac gaagatcatg tcgccgcgcc ccagcagcgt
     3061 ctcggccccg ttggcgtcga gcaccgtgcg cgagtccacc ttggaggcca tctggaaggc
     3121 gatgcgcgtg ggaaagttcg ccttgatgag cccggtgacg acgtccaccg acgggcgctg
     3181 ggtggcgacg atcaggtgga tgccgatggc ccgcgcgagc tgggccaggc gcacgagcga
     3241 ggtctggacg tcgcccgcgg agatcagcat gaggtcggcc agctcgtcga tgaccaccac
     3301 ccagtacggc agccgctcgg cgggctcgac gaccttgttg tagccctcga tgctccggac
     3361 ctgcttgagc tggagctgct tgtagcgctc gtccatcttg ccgacgatcc agcgcaggcg
     3421 cgcggcggcc tccttggcgt cggtgacgac gggcgccagc aggtgcggga tgccctcgta
     3481 gaccgacagc tcgaggcgct tggggtcgat catcaggaag cgcacctcgg ccggcgtcgt
     3541 cttgtagagg atcgagcaga tcatcgcgtt gaggcccacc gacttgccgg tgccggtggc
     3601 gccggcgacg agcaggtgcg gcatcgccgt gagatcggac accacggcgc tgcccgtgac
     3661 gtccttgccc agcgccagcg gcagccgccg cctggggtcg gcgaagtcgg gcgacgcgaa
     3721 gatctcccgg aggtagacca tcgccgactc ggcgttcggc acctcgacgg cgacggtgcc
     3781 gcgcccgggg atcggcccca cgatgcgcac cgaggccgcc ttgagagcca gcgccaggtc
     3841 gtcggcgagg ttcacgaccc ggctgatctt cacgcccgcc gccggctcga actcgtagga
     3901 ggtgatgatc ggtccggggc tcacctgcac gatgcgaccg tccacctcga agtcctggag
     3961 cttgcgccgc agcgtctcgg cgttgtcgtg gagctcctcg tgggtgatct tgtgctcgga
     4021 ggcgccgggc acctgcagca gccccaccgg cggcagctgg aacgccgcgg cgccgccggt
     4081 gccgaagtcg aacgtctcct gccaggccag cgccgtgctg ggcacgcgct tgcgggactc
     4141 cacgaccacc ggcgggggca ccgcctcctc gacggcgatc acggcgtccg tcggcagcgc
     4201 ctcgtctccc gggatcccgg acgtaccgcc ggcggagcgc ggcggcctcg cgagggccgc
     4261 ccgccgcttg cgccagcgcg ccagccgcgc cgacaccaga cgcgaggccg ccccgtagga
     4321 gacctgggtg acgcagagga cgccgacggg cacggccgcc agcagcacga gccaggtacc
     4381 cgcgccgccg acggtggcct ggagcccctc cacgagccag tagccggcga tccccccgcg
     4441 catcacgacg tcgcgggcct ccgggccggt gaaggtgttc gccgactccg cgagcaggcc
     4501 ggtggcgctc agcacgagca tggcgatgcc gatgaccggt gccccgcctc ggcccaccag
     4561 tgggcgcagg aaggcggaga ccgcgaccac ggcgaggagc agcggcacga ggaagcccgc
     4621 gtagccgagc gaccggaaga gcgcccagcc gagccacacg ccgaccgggc cggcgggccc
     4681 gcgctgctcg gcggccggta gcccggggtc gaaccacgcc agcgcgacga acgcgaaccc
     4741 ggcgacggcc agcagcgcga tcgccttcac ctcgcgcacc cagcggcgcg tctccctctt
     4801 cttgcgcgca cggcggggct tgtcgtcggc cgtgacgatc gtcgccatca gacctccagg
     4861 atcaccggca gcaccacggg cttccggtga aaccgctggt tgatgaactg ccgcaccgcc
     4921 gcgcgcacga gggcgccgag cccctcgcgg tccgcaggcg cgctggcgtc gcgcgccgcg
     4981 agcacggcgc gcaccgcccc ccgcagctcc tcgagcagca cctcgttctc cttcacgtac
     5041 acgacgccgc gcgaggcgat ctcggggccg gccaccagcg cgccccggcg gtccacgacc
     5101 agcgagaccg agatgatccc ctcctcggcg aggacctggc ggtcgcgcag caccacggcc
     5161 ccgacgtcgc cgacgccctt gccgtcgacg agcacgcggc ccacggggaa gcgcccgacc
     5221 acgcgcgcgg ccgtcttggt gagctcgagc ccgaggccgt cctcgatgag gaagacgtgg
     5281 tcggccggca gcccggcgcc ctcggccagc cgcgcgtggc ccagcaggtg ccggtactcg
     5341 ccgtggaccg gcacgaagta gcgcggccgc gtgagggtca gcatccgctt gaggtcctcc
     5401 tggctcgcgt ggccggagac gtgcacgaag gcgttgtcct cccagagcac ctcggcgccg
     5461 agccgcaaga gcgcgttgac cacgcggccg atcgtgcgct cgtggccggg gatgacgcgc
     5521 gccgagatga tcacgaggtc gccgcgctcg acctggaccg ccttgtgctc gccggcggcc
     5581 atgagcgcga gggccgagtt cggctcgccc tggctaccgg tggagaggat cacctgccgc
     5641 gacgacggca gcgcgaccag atcctcgagc gcgagcagga cgcccggcgg cacccgcaga
     5701 tagccgagct ccgaggcgat cgcgacgttc ttctgcatgc tcatgccgag cagcgcgacc
     5761 gagcgcccat gctcagcggc gagcgtgagc acctgctgga tccggtggat gtgcgaggcg
     5821 aacgtggcca ggatgacgcg tccgcgcgcc tgtcggaacc gctcgcggag cgcctggccc
     5881 acctcgatct ccggccgcgt gtggcccggg cggtcgacgt tggtcgagtc cgagcagagc
     5941 agcagcacgc cctgctcgcc cagctcgtcg aagcgccgga agtcgggcgg ctcgccgtcg
     6001 agcgggctcg ggtcgagctt gaagtcgccc gtgtgcacga tggtgccgac cggcgtgtcg
     6061 atggccagcc cgatgccgtc cacgatcgag tgggtcacgc ggatcggctc gacgcggaac
     6121 ggcccggcct cgatggcgtc gcgcggccgc atggtccgga gatcggcgat ctcgaggagc
     6181 ccgtgctcgc gcagccgctc ggccacgagc gcgagcgtca tcggcgtacc gtagacgggc
     6241 acccgcacct ggcggagcag gtacggcagc gcgcccacgt ggtcctcgtg gccgtgggtg
     6301 aggaacaccg cgcggaaacc gtcgcgcttg ccgagggcgt aggcgaagtc ggggatcacg
     6361 tggtcgatcc ccggcatctc gtcggggtcc gggaacatga gcccgcagtc caccgcgacg
     6421 aggtcgtcgc cgaactcgac gagcatcatg ttgagcccga tctcgccgag gccgcccagg
     6481 gggatcagcc ggacggccgg ctcggtcaga ggctccacgg aaactccttt cgttacagcc
     6541 gaacatggta gcaccgcggt cagtccccct gaaatttcac cacggccggc gaggagtccc
     6601 gaggaccggt tctcgggacg gccttcagaa gggggcttcg tcctccaggc gggagagaag
     6661 cgacttcgcg tagcgggaga actcggcctt cccggcggcg ggaatgggct cgccggggat
     6721 gaacttctcg ctcatcgggt tcacgaagac gccgcccttg gccacgcggt aatcgaggtg
     6781 cggacccgtc gccagcccgg tggcaccgac gtagccgatg acctggcgct gcgtcacgcg
     6841 cgcgccgcgc cggatgccgg cgcccacgcg cgagaggtgg ttgtacagcg tctcgtagcc
     6901 gctccggtgg cgcaggtgca cctggatgcc gttgccgccg ttccatcccg cgcgcaccac
     6961 ggcgccgtcg gcgacggcgc gcacgggcgt gcccaccggc gccccgtagt cgatggcgag
     7021 gtgcgggcgc acgccgccca ggatcgggtg cgggcgcgcg taggtgaagc ccgaggtgat
     7081 ccgggagaac tcgaggggcg acttgaggaa gctcttgcgc atcgcgcgcc cgccgaggtc
     7141 gtagaacgcg aacttggcgc cgggctgcgc gaagccgatg ccggtgagcc ggcgcccgcc
     7201 gctctcgtac tgcgcggcga gcacgcggcc gtagttgacg aacgtctcgc ccgcgtagcg
     7261 cttctcgacg agcaggcgga agcggtcgcc ctcgcgcgtg tcggcggtga agtcgaagtc
     7321 cgactcgaag atggccacga gcgccagcac gagcggtggc gtctcgccgg ccacgtcgat
     7381 cgcctcgaag agcgaccgca cgacaatccc ttgcaccgtc tgcacgcgca cgtcgggcgc
     7441 cgtctccagg cgcttcacgc tccagccgtc ctcgtcggcc accgccgcgt agctgcgcca
     7501 cgggctcgcc tgccaggtga cggcggtcgg ctggtccgcg gggctccagg cgatctccag
     7561 gtcgtcaccc ggcctcagcc gacgcaggtc ggcgccggcc ttcttcagcg cggcggcgac
     7621 ctcgtgggcg ggccgggacg gcacgccggc gcgccggagc gcggtgacga gcgtgtcgcc
     7681 gcgcttgagc gagaccttct cgaggcgatc gaccgccgcc gcccgagcgg gctcaggggg
     7741 ctccgtggcg acgacgggca cgggcggcgg ggggggcgct gtgcgctgcg ccagtgccgc
     7801 cgcggccaat ccggcgacca ggaacgacgc caggaccacg agcctgaagc gtgagggcat
     7861 ccatctcatg atagccggcc cgcgaggctg gcgaactcgg aaatcgacag ggtctcggcc
     7921 cggcggcggg ggtcgatccc cgccgcctcg gcggcctcgc gggcgctctc cgcagggatg
     7981 ccgagccctc cggacagcgc gttggccagc atcttccgcc gctgggcgaa ggccgcgcgc
     8041 acgacggcct cgaagcggcg ctcgtcggcc agcgcgaccc ggggtccggc cagcgcgcgc
     8101 aggtggagga cggcggactc cacctcgggc gggggccgga aggcgcccgg cggcacgcgg
     8161 agggccacgc gcacgtccca gtggagctgg cagaggatcg agaggctcgc gtaggccttc
     8221 ccgccggggg cggcggcgac gcgctcggcc acctcgcgct ggagcatcag cgccatctcg
     8281 gcgatcgctc cgtgcgcctg gaccagcgcc agcaagatcc tcgtgccgac gctgtacggc
     8341 aggttgccga cgacgaggac gcgcccgccc ggctcggcgc gcagcgtggt gtagtcccac
     8401 tcgcgagcgt cggcctccag cacctcgacg ccggggaagc gctcgcgcag gcgcgggacg
     8461 agcgcgcggt cgacttccag cgcggtgatc cgcgccgggt gtcgcgccag ctcggccgtc
     8521 agcgcgccct cacccggccc gatctcgacg acgacgtcgc gctcggccgg cgcgaccaga
     8581 tcgacgatcg cgcgcgcgat gccggcgtcg cgcaggaagt gctggccgag ggcacggcgg
     8641 cggctcactt ccccttcagc ttgacgtcct cgtacggcgc ggagaacgcc cagcccgtga
     8701 tgagcccgag gcccgactca gccacccggg ggccctgcgc gtggatgaag cccagctccc
     8761 agatcggcgc gtacatcgcc ttgtcgtgga cgaggttctg gatccggtgc agcatcgcct
     8821 cccgccgctt ccggtcgagc tcgccagcct gctcccggaa gagcccgtcg atgtcgggat
     8881 agctgccgta gacgtagggg ccgcccgcgg ccacgaagac ctcgaggcgc gtggccgcgt
     8941 tcccgaaggc gccactgata ctgtagagga ggttcttcag cttcttctcc tggtaccccc
     9001 tgaagaaggc cgcccgctcg agcggcctga ccctcgcccg gatccccacc gcctgcagaa
     9061 acccggcggt cgcctcgctc atggtggcgg tggcggcgtc gcaccagagg tccccggcgt
     9121 cgaagccttt cgggtagccg gcctcggcga ggag
//
LOCUS       DATIAZ010000289         7274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_873062, whole genome shotgun sequence.
ACCESSION   DATIAZ010000289 DATIAZ010000000
VERSION     DATIAZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873062"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VLF19_10125"
     CDS             <1..345
                     /locus_tag="VLF19_10125"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSE93652.1"
                     /translation="FEGPTFNLRTEAGLAWVYEDYDTTGSRDFIAGRLAYAVDWTPTP
                     PVTLFHTLEYLPSFEDFTGDYLLNIDAGLRTRMWRGLFGEFKVEYRYDATPAPGRENA
                     DTRLLLGLGWEF"
     gene            complement(488..1126)
                     /locus_tag="VLF19_10130"
     CDS             complement(488..1126)
                     /locus_tag="VLF19_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93653.1"
                     /translation="MPRRRVLAVIVWLVTALHHAAAPGAALDTLLAAVDDKTVAASDV
                     ALARGLGVLGFTPSTGPIARADLERFVDVLLVLEEAGRIGIAVEPAETDRAWATAAAR
                     AGGEVALDRWLDAHAIDRAWARRLVEADLLRARFLHERFAAFVFPDEEQIARELGPGQ
                     QDETARERVRERLVREGAERAQTEWLAEVRRRALIRILIPAGAAIPPPFSPP"
     gene            complement(1153..3903)
                     /locus_tag="VLF19_10135"
     CDS             complement(1153..3903)
                     /locus_tag="VLF19_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSE93654.1"
                     /translation="MMRRLLAALAALVPVLVTSAFAAPAPPVVTGVEVTSPHVLPAPS
                     PQQLLTGLVGRPLSRAAVRDSLDRLWGLGVLESVEVETVPEPGGVRLRYRVSRHPHLE
                     RLDWSGDLGLSAPDLAATAALALGGPADAARLDRARDEVRGRLQREGYFGATVRLDVG
                     ENPASNGRAVTFVVDSGKPARVGRVELIGLVRAEAEPLRKALALDEGDVFRERSFRDG
                     ERALAEGLHAQGFFEARVTAREPAWDRATNRVNLTVQVAEGPLTRVEFSGREALTEKA
                     LRERLTFADSGAVDEIEVRASAEQIERAYRQAGHHFVRVTGTLGGDPAARVASFAIEE
                     GPRVIVESVAFEGLTALSAERLLEQMRTRSAGLLRRGLFVEETLAQDVRNLRQYLKSQ
                     GYGEAEAGPPRTTFSDDRTRAAVVIPVVEGPRRIVAAVSLTGNRAVASEQISKAIGLR
                     PGDPWDEIRSDEARRKVEQFYQRRGYDGTTVGVTTTETAGAVAVTVAIEEGALARVGQ
                     VLISGITVTRPSVVRREIPFGPGDPLTAVDLAEARRRLDATRIFDRVDVDVRGPAGAP
                     FRDVEIALREARPWRFEFGLGYATEEGFRGFVSLSHDNLFGTGRSASLSERVSERSER
                     TELQYREPWILGSAWQGEAVAFRERKDEIGFVTDRAGMTFTAQRDLLTPLFRPEEPTD
                     HPRSLRGGLRYRLEQFQRSDIEPELLVEGTTERDERVGSVIPFLTLELRDNPTDPRSG
                     SYHYTSLEVGNTVLGGEVNFLKFRLESSWLVAWPPPTVLAISTRLGLAGPYGGSDDLV
                     IEDRFKAGGSTTIRGYRQDRVGPLDAAGNPEGGDLAAILNIEWRFPIWRFLGGATFVD
                     VGTVMPRVRDVSVASLFPGVGGGLRITTPIGPLRLDVGYGLRQIRDEDRLQVYLTVGH
                     AF"
     gene            complement(3900..>7274)
                     /locus_tag="VLF19_10140"
     CDS             complement(3900..>7274)
                     /locus_tag="VLF19_10140"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HSE93655.1"
                     /translation="LNRGRLSYEDPALAVRVEAEGLAATLWPGREAMSATLAAREIRV
                     DAPTLRERAEQLEAEIRITPARLEARRLEGSWEKRRMAVTGRVDGPFDAPTVDLTARG
                     DVDLATLGRRAGSPWRLAGLARASARLEGPAAAPRVTGNVALDELTAGPVKARAVMAR
                     LAFADGVLSVTQLGARAFDGSVSGTAVLEPAHLERARVTLSLRGVSSTALEKIAELET
                     GVTARLDAEADVRGDLRDLSRVHARARVNAREVRLPGAFASLGAGTVDAEATGDQGRF
                     DLSRGIAAWPGLQLEARGQATLDAANPVHVKATVDLARLAPLLGSIHVAGDAVLEGQV
                     TGRWRDPVLAGKLDVRSPAVAVVRADEAAASFTLTRRSLRLGAASARLGQARLAVSGN
                     LAWPGSASPAVPEPRVVSIDLLARTENAHVEDAAPWLPPALHGSSGPVEVTARIDGTL
                     TAWRVVGQAESASLSVPSAPPVRATRVSFEATPERLEVPALRARVLDAPVTAKGRWRW
                     AGTGDVEAEAGPVDLARVPHVPEGLSVEGRARAHLTATLRDSRLAGSGRILGESVGVA
                     GVRLGHGVADLSMDATAVRAEVTFPEARVAATGRGRLDGAALLATTLTATDIEIGPLL
                     ARFRPDLEGTITGRFSAVATLDVPAREPRATRGVIRLEPVRFEAAGERWETRGPVLVR
                     REPGRTSVERLEVAGRLGSATATGWLGDGGTVEATVRGQAPLALLSVFRPEIREASGR
                     LDVDVRVGGTTAKPIVLGRGTIVDGLIALRDTPIVVRDIEGRVALAPARLRIEELRAT
                     VGTGTVRASGEVGLDGRALGAYQLSLTGRGLSVAALEGLETVWNADLTLVGRETRALV
                     RGEAHLVRGTYTRDLSILPVLLEGGARAQPVEWGREIALQIDVHLDDNLVVRSPQARL
                     RAGGDLRLRGTVAQPVILGTVQTQDGHITFRRNRFTLENAVVRFDDPRRLNPYLDVRA
                     TTRIRTYDVTMRLTGRADDLTVRLSSEPPLPQQDLLALVTLGATRAELGTSGGLTFAG
                     EAAQLLTREVLGIEASAPLVDIVEFGRTEEGQNQFRVGKRLDDRTTVIYSGSFAEGGQ
                     QKLRIEYQLIGPLLLAGEQVFTGGFGGDVILRLRFR"
BASE COUNT          999 a         2713 c         2618 g          944 t
ORIGIN      
        1 ttcgagggtc ccaccttcaa cctccgcacg gaggcgggcc tcgcgtgggt ctacgaggac
       61 tacgacacca ccgggagccg cgatttcatc gccggccggc tggcctatgc cgtggactgg
      121 acgcccaccc ctccggtgac cctcttccac acgctggagt acctgccgag cttcgaggac
      181 ttcaccggcg actacctgct gaacatcgac gcgggcctgc gcacccggat gtggaggggc
      241 ctgttcgggg agttcaaggt cgagtaccgc tacgacgcca cgccggcgcc cggccgcgag
      301 aacgccgaca cccgcctcct cctcggcctc ggctgggagt tctgaggccg ccggcgcagg
      361 cagcgtgcct ttctccggcc gggcgttcac cttgctcgct gctctacctg atcgccacgc
      421 gggtcatcgc gcgagggtct tcgagatcgc atccgcctgc ggcgcgagag cgagaacaag
      481 tggctcgcta cggcggcgag aacggcggcg ggatcgcggc gccggccggt atgagaatcc
      541 ggatgagcgc tcgccggcgg acctccgcca gccactcggt ctgggcccgc tccgcgccct
      601 cgcgcacgag acgctcgcgg acccgctccc gtgccgtctc gtcctgctga cccgggccga
      661 gctcccgggc gatctgctcc tcgtcgggga agacgaaggc agcgaatcgc tcgtggagaa
      721 agcgtgcgcg cagcagatcg gcctcgacga gacgccgcgc ccaggcccga tcgatggcgt
      781 gcgcatcgag ccagcggtcg agcgcgacct ccccccccgc gcgcgccgcc gcggttgccc
      841 aggcgcgatc ggtctcggca ggctcgacgg cgatcccgat ccggccggcc tcctcgagga
      901 ccagcaggac gtcgacgaag cgctcgaggt ccgcccgagc aatgggaccg gtcgaagggg
      961 tgaacccgag gaccccgaga cctcgggcca gcgcgacgtc gctcgccgcg acggtcttgt
     1021 catcgaccgc ggccaggagc gtgtcgagcg ccgcgcccgg cgccgccgcg tggtgaagcg
     1081 ccgtgaccag ccacacgatc accgcgagga cacggcgtcg aggcacgccg ctcggctcga
     1141 gccccggccg catcagaatg cgtgcccgac cgtcaggtac acctggagtc ggtcctcatc
     1201 gcggatctga cggagcccgt accccacgtc gaggcggagc ggaccgatgg gagtggtgat
     1261 ccgcaggcct ccgcccactc ccgggaataa ggacgccacg gagacgtcgc ggactctcgg
     1321 catgacggtg ccgacgtcga cgaacgtggc gcccccgagg aagcgccaga tcgggaagcg
     1381 ccactcgatg ttcaggatcg cggcgaggtc gccgccctcg gggttgccgg ctgcgtcgag
     1441 gggtcccacg cgatcctgcc ggtagccgcg aatggtcgtg ctcccgccgg ccttgaagcg
     1501 gtcctcgatg acgaggtcgt cggagccccc gtacggcccg gcgagaccga gccgcgtgga
     1561 gatcgcgagc accgtgggcg gcggccaggc gacgagccac gagctctcga gccggaactt
     1621 gaggaagttc acctcgcccc cgaggaccgt gttgcccacc tcgagcgacg tgtagtgata
     1681 gctgccgctc cgcgggtcgg tggggttgtc gcgcagctcg agcgtcaaga agggtatcac
     1741 gctgccgacg cgctcgtcgc gctcggtggt gccctcgacc agcagctcgg gttcgatgtc
     1801 gctccgctgg aactgctcga ggcggtaccg gaggccgccg cgcaggctcc ggggatggtc
     1861 ggtgggctcc tccggacgga agagtggggt caggaggtcg cgctgggcgg tgaacgtcat
     1921 tcccgcgcgg tcggtgacga agccgatctc gtccttccgc tcgcggaagg ccacggcctc
     1981 gccctgccag gcgctgccga ggatccacgg ctcacgatac tggagctccg tgcgttcgct
     2041 tcgctcgctc acgcgctcgc tcaggcttgc gctccggcca gtgccgaaca ggttgtcgtg
     2101 gctgagagag acgaagcccc ggaagccctc ctcggtggcg tacccgagcc cgaactcgaa
     2161 gcgccagggc ctcgcctccc gcagcgcgat ctcgacgtcg cggaacggcg caccggccgg
     2221 gccgcgcacg tccacgtcca cgcggtcgaa gatgcgcgtc gcgtcgaggc ggcggcgcgc
     2281 ctccgcgaga tcgacggcag tgagcggatc gcccggcccg aacgggatct cgcggcggac
     2341 cacggagggc ctggtgacgg tgatcccgga gatcagcacc tgtcccaccc gcgccagcgc
     2401 tccttcctcg atggcgacgg tcaccgcgac cgcgccggcc gtctcggtcg tggtcacgcc
     2461 gacggtcgtg ccgtcgtagc cgcggcgctg gtagaactgc tccaccttcc ggcgcgcctc
     2521 gtcgctccgg atctcgtccc agggatcgcc ggggcgcagc ccgatcgcct tcgagatctg
     2581 ctcggacgcc accgcccggt tgccggtgag gctgaccgcg gccacgatcc gccggggtcc
     2641 ctcgacgacc gggatcacga ccgcggctcg cgtgcggtcg tcgctgaacg tcgtccgggg
     2701 cgggcccgcc tcggcctcgc cgtatccctg ggatttcagg tactggcgca ggttgcgcac
     2761 gtcctgggcc agggtctcct ccacgaagag gccgcgcctg agcagcccgg cggagcgcgt
     2821 ccgcatctgc tcgagcaggc gctcggcgga aagcgcggtg agcccctcga acgcgacgct
     2881 ctcgacgatg acgcgggggc cctcctcgat cgcgaagctc gccacgcgcg cggccgggtc
     2941 gccgccgagc gttcccgtca cgcggacgaa gtggtgccct gcctggcgat aggcgcgctc
     3001 gatctgctcg gcgctggcgc gcacctcgat ctcgtccacc gccccgctgt cggcgaaggt
     3061 cagtcgctct cgcaaggcct tctcggtcag ggcctcccgt cccgagaact cgacccgggt
     3121 cagcggtccc tcggccacct gcaccgtgag gttcacgcgg ttggtggcgc gatcccaggc
     3181 cggctcgcgc gcggtcacgc gcgcctcgaa gaagccctgg gcgtggaggc cctcggcgag
     3241 cgcgcgctcg ccgtcgcgga aggaccgctc gcggaagacg tcgccctcgt cgagcgccag
     3301 cgccttgcgg agcggctccg cctccgcgcg cacgagcccg atcagctcca cgcggcccac
     3361 gcgcgccggc ttgcccgagt ccacgacgaa ggtgacggcg cggccgttgc tcgcgggatt
     3421 ctcgccgacg tcgagcctca cggtggcccc gaagtagccc tcgcgctgta gtcggccgcg
     3481 gacttcgtca cgcgcgcgat cgagccgcgc ggcgtcggcc ggcccgccca gggccagggc
     3541 ggccgtggcg gcgaggtcgg gggcggacaa gccgaggtcg ccactccagt cgagccgctc
     3601 gaggtgcggg tgtcgcgaaa ctcgatagcg gagccggacc cctccgggct cgggcaccgt
     3661 ctcgacctcc acgctctcga gcacgccgag cccccacaga cgatccaggc tgtcgcgcac
     3721 cgccgcccgg gaaaggggcc ggccgacgag gcccgtcagg agttgttgcg gtgaaggcgc
     3781 gggcaggacg tggggcgacg tcacctcgac accggtgacc acgggcggcg cgggggccgc
     3841 gaaggccgag gtcacgagga cgggcacgag cgccgccagg gccgccagga ggcggcgcat
     3901 catcggaagc ggaggcgcag gatcacgtcg ccgccgaacc cgcccgtgaa gacctgctcg
     3961 ccggccagca gcagcggccc gatcagctgg tattcgatgc gcagcttctg ctggcctccc
     4021 tcggcgaagc tccccgaata gatcacggtg gtacggtcgt cgaggcgctt gcccacgcga
     4081 aactggttct gcccctcctc ggtgcggccg aactcgacga tgtccaccag cggggcgctg
     4141 gcctcgatgc ccagcacctc tcgggtgagg agctgcgcgg cctcgccggc gaaggtcagc
     4201 ccgcccgagg tccccagctc ggcgcgggta gcgcccagcg tgaccagggc caggagatcc
     4261 tgctgcggca gcggcggctc ggacgagaga cggaccgtca ggtcgtcggc gcggccggtc
     4321 agtcgcatgg tgacgtcata ggtccggatc cgggtcgtgg cgcgcacgtc caggtacggg
     4381 ttgagccgcc gcgggtcgtc gaagcgcacg acggcgttct cgagcgtgaa gcggttgcgg
     4441 cgaaacgtga tgtgaccgtc ctgcgtctgg accgtgccca ggatgacggg ctgggccacg
     4501 gtgccgcgca ggcggagatc gcccccggcc cggagccgcg cctggggcga ccgcacgacc
     4561 aggttgtcgt ccagatggac gtcgatctgg agcgcgatct cgcggcccca ctcgaccggc
     4621 tgcgcgcgcg ccccgccctc gagcaggacc ggcaggatcg agaggtcgcg cgtgtaggtg
     4681 cctcgcacca gatgggcctc gccccgcacg agcgcgcgcg tctcgcgtcc gaccagtgtg
     4741 aggtccgcat tccagacggt ctcgaggccc tcgagcgcgg ccaccgacag accgcggccc
     4801 gtgagcgaga gctggtaggc gccgagggcc cggccgtcga gcccgacctc tccgctcgcg
     4861 cgcacggtgc ccgtcccgac ggtggcccgc aattcctcga tgcgcaggcg cgccggtgcc
     4921 agcgcgacgc gcccctcgat gtcgcggacc acgatcgggg tgtcgcgcag cgcgatcagc
     4981 ccgtcgacga tcgtgccgcg gcccagcacg atcggcttcg cggtggtccc gccgacgcgg
     5041 acgtcgacgt ccagccgtcc cgatgcctcg cggatctccg ggcggaacac ggacagcagg
     5101 gcgagcggcg cctggccgcg cacggtcgcc tcgaccgttc cgccgtcgcc gagccagccg
     5161 gtcgccgtcg cgctaccgag ccgtcctgcc acctccaggc gctcgacgct ggtccgcccg
     5221 ggctcacgac gaacgaggac cgggccacgc gtttcccagc gctcgcccgc cgcctcgaac
     5281 cggaccggct ccaggcgaat cactccccgc gtggctcggg gctcgcgggc cggcacgtcg
     5341 agggtggcga ccgccgagaa ccgtccggtg atggtgcctt cgagatcggg tcggaatctg
     5401 gcgagcaacg gcccgatctc gatgtcggtc gccgtgagtg tcgtggcgag gagcgctgcg
     5461 ccatcgagac ggcctcgacc ggtggccgcg acccgcgcct cggggaacgt cacctccgcg
     5521 cgcaccgccg ttgcatccat ggagagatcg gcgacgccgt gcccgagccg aacacccgcc
     5581 acgccgacgc tctcgccgag gatccggccg gagccggcca gccggctgtc acgcagggtc
     5641 gcggtcaggt gcgcccgcgc gcggccctcc acgctcagac cttccggcac atgcggcaca
     5701 cgcgcgagat cgacgggacc ggcttcggcc tcgacgtctc cggtgcccgc ccagcgccag
     5761 cggcccttcg ccgtgaccgg ggcgtcgagc acccgcgccc gaagcgccgg aacctcgagc
     5821 cgctccggtg tcgcctcgaa ggagacgcgc gtggcgcgca ccggcggggc cgacggcacg
     5881 ctgaggctcg cggattcggc ctggcctacc acgcgccagg cggtgagcgt accgtcgatt
     5941 ctcgcagtga cttcgaccgg accgctgctg ccgtgcagtg ccggcgggag ccagggggcc
     6001 gcatcctcca cgtgcgcatt ctccgtgcgc gcgagcaggt cgatcgacac gacccgaggc
     6061 tccggcaccg ccggagaagc ggatccgggc cacgcgaggt tcccggagac agcgaggcgc
     6121 gcctgtccca gacgcgcgct cgcggccccc agacggagcg agcgccgggt cagcgtgaag
     6181 gacgcggccg cctcgtcggc gcggacgacg gcgacggccg gcgagcggac gtcgagcttg
     6241 ccggccagca cgggatcacg ccagcgtccc gtcacctgtc cctcgaggac ggcgtcgccg
     6301 gcgacgtgga tggagccaag cagcggagcc agccgtgcga ggtcgaccgt cgccttcacg
     6361 tgcacggggt tcgcggcgtc gagcgtggcc tgccctcgtg cctcgagctg gagacctggc
     6421 caggcggcga taccacgcga cagatcgaag cggccttgat cccccgtggc ctcggcgtcg
     6481 acggtccccg cgcccaggga ggcgaacgcc cctggaagcc gcacctcccg cgcgttgacg
     6541 cgggcacggg cgtggacgcg cgagagatcg cgcaggtcgc cgcgcacgtc cgcctcggcg
     6601 tccaggcgcg cggtcacgcc ggtctcgagc tccgcgatct tctcgagcgc ggtgctcgag
     6661 accccgcgca ggctgagcgt gacgcgcgcg cgctccagat gcgccggctc gagcacggcc
     6721 gtgccgctga cggagccgtc gaacgcccgc gcgccgagct gcgtcaccga cagcacgccg
     6781 tcggcgaacg cgaggcgcgc catcacggct cgcgccttca ccgggccggc cgtcagctcg
     6841 tcgagcgcga cgttccccgt cacgcggggc gccgcggccg ggccttccag ccgtgcgctc
     6901 gcccgcgcca atcccgccag ccgccagggc gagcccgctc gccggccgag ggtggccaga
     6961 tcgacgtcgc cacgcgcggt cagatcgacg gtgggcgcat cgaaggggcc atcgacgcgg
     7021 cccgtgacgg ccatccgccg cttctcccag gatccctcga gacgccgggc ctcgagccgc
     7081 gcaggcgtga tgcggatctc ggcctcgagc tgctccgcgc gctcccggag cgtcggcgca
     7141 tcgacccgga tctcgcgcgc cgcgagcgtc gcgctcatcg cctcgcggcc aggccacagg
     7201 gtggcggcga ggccctcggc ctcgacgcgt acggcaagcg ccgggtcctc gtaggacagc
     7261 cgcccccgat tcag
//
LOCUS       DATIAZ010000290        14498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1529543, whole genome shotgun sequence.
ACCESSION   DATIAZ010000290 DATIAZ010000000
VERSION     DATIAZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529543"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1486
                     /locus_tag="VLF19_10145"
     CDS             290..1486
                     /locus_tag="VLF19_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSE93656.1"
                     /translation="MTQRGFVKLLSIALLALLAWPALAAAQGVKVGAVVPLTGRYGAG
                     GAQVRAGYEIGVEHVNARGGVSVGGTKMPLELTILDDESDATKTVARLETLAAQGVVA
                     YLGGFGSDLHAAAASVAEKNKTPYLGVAFALHKIHTQGFRYLFSPFWKSPDIKEQTVN
                     LLGGLPAAERPKRAAIFQEKTDWGREMATAWTEAAKAAGYAVVVNGEYAPGARDFSDI
                     ILKAKGANADIVFGLPTPPDGMTIVKQMKELGFSPKMTLLIRAPDAPVWSKNLGKDGD
                     YVVLAPGWHHGVKAPGVKELNEAHQKKVGRPADPIAGPAYACVQILAAAIDKAGSLDR
                     DKIRDAIAATSMTTVVGPVKFRPDGTGVVNAVFLQWLNGKQELVWPKDAATAALVYPA
                     PPFAKR"
     gene            1506..2246
                     /locus_tag="VLF19_10150"
     CDS             1506..2246
                     /locus_tag="VLF19_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93657.1"
                     /translation="MRLLEVERLTRSFGGVRAVSEVTFAVDEGEVIGVMGPNGSGKTT
                     LFNLITGALRPDAGAVRLKGTDITGWAPHRVCAAGIARTFQLVRPFPGLTALDNVLVG
                     RLYGRERGDRGSATAEAGRLLALVGLDGKAGRLAAELTLMDRKRLELARALATSPHLL
                     LLDELMAGLNPAETEAAMALIRRLQSEGVTLLMVEHIVWALMELSQRLVVLSAGEKVA
                     EGSPATVAADPTVIDIYLGSAAGSVRRA"
     gene            2239..2958
                     /locus_tag="VLF19_10155"
     CDS             2239..2958
                     /locus_tag="VLF19_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93658.1"
                     /translation="MLEVEGVEASYGPFRALAGVTLTVRPGEIVAILGPNGAGKTTLI
                     RCVAGLLRPRAGVIRWEGAPLTGAPAHAIVERGIAVVPEGRRLFASMTVEEHLDLGAF
                     APRARAERARRLAEVYAIFPVLRERRRQVVQALSGGQQQMVAIGRALMASPRLLALDE
                     PSLGLAPRVVDSILGVLAQINRAGVSILLVEQNVHAALALAHRAYILESGRVAGEGAA
                     AALLDDPHVRRAYLGPLAARA"
     gene            2955..3830
                     /locus_tag="VLF19_10160"
     CDS             2955..3830
                     /locus_tag="VLF19_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93659.1"
                     /translation="MTPAVLLQQLVLGLLIGGLYGLAAAGLSLVFGVLKVLNVAHGEL
                     IMLGGYGAFWLFALAGIDPFVALTLVVPASLVLGALLYQGLFAWVVRAHHEARIKNSL
                     LIGFGLGLALHAAAVLLWTADERSVTTDYGGRVIELGGIYLPVVRLASLVLAFAITAG
                     LHLLLARWRWGRAIRATADDWEAAAMTGIDVRRAYLLAFALGTGLAGAAGTLVSVGYS
                     ISPSIGLEWTLKALIVVVLAGLGSMLGTFVGGLVLGLAEAVSATLFGGPYREVVGLII
                     FFVVLVARPQGLFSR"
     gene            3857..4741
                     /locus_tag="VLF19_10165"
     CDS             3857..4741
                     /locus_tag="VLF19_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93660.1"
                     /translation="MAAVAALAWVPLVVRRDDVVNFLVLTLLTITLAQSWNIVAGYAG
                     QVNLGHAAFFGLGALVTRTLWIADVAVPLAMAAGAAVAVAFALAVGAAAFRLRGAYFA
                     IGTLALGEILRITVGNVLAEISTLPTTAIAGYRLAFRYWLALGLAALSVAAVAVLAGS
                     RLGLGMQAIREDEEAAEATGVGALGLKLIALVLSTALAGLAGGLFAYYHISYYPSHPF
                     SPHWTFDALLVTFIGGVGTLHGPILGAVFYVFLKEYLAIRWVDFHLLIFGALFVAIVL
                     LLPGGLVEAGARVRRLRA"
     gene            4918..5202
                     /locus_tag="VLF19_10170"
     CDS             4918..5202
                     /locus_tag="VLF19_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93661.1"
                     /translation="MNTLVTQFSPIETSDGGVYSAQACGVQRDDGLWEGWLAFFDAHG
                     RAWRAGRETVQPSQAALEYWASGLTPVYLEGALNRAVRVAPVAAVTPETD"
     gene            5290..6156
                     /locus_tag="VLF19_10175"
     CDS             5290..6156
                     /locus_tag="VLF19_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HSE93662.1"
                     /translation="MRWTMGRRSENVEDRRGRGLPLVAGGGIGTIVILLLALFFGLDP
                     GVLLQTGAPPSDRTQAPEATGGHDEMRDFVSVVLADTEDTWRTLFRSMNREYRDPTLV
                     LFTGRVQSACGIAGAAVGPFYCPGDHKLYLDLAFFRELRDRFGAPGDFARAYVIAHEV
                     GHHVQTLLGISERVMATRQRVGETEANALSVRLELQADCFAGVWAHHADRARQVLEAG
                     DLEEALAAAAAIGDDRLQRQTQGHVVPESFTHGTSGQRVTWFRRGFDSGDVRRCDTFT
                     AGGSGPERPTRR"
     gene            6268..6429
                     /locus_tag="VLF19_10180"
     CDS             6268..6429
                     /locus_tag="VLF19_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93663.1"
                     /translation="MNPRSFALAPLWLRLVLLPLSLLAAAAAPALAADAPDVASAPDV
                     ATAPSSSTA"
     gene            6525..6920
                     /locus_tag="VLF19_10185"
     CDS             6525..6920
                     /locus_tag="VLF19_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93664.1"
                     /translation="MATSTLRVVPAERSSDIVMGDHPIMSVFDADAEIERLNRQDLAR
                     SYANRIRVVVEAYCRDRSPRSLLVGAAASLAATAALVGTVLLLLRFWRRLSVVLEPRY
                     TRRLRSLEIQTPHCTGTSRTCSTSTACRS"
     gene            7253..8401
                     /locus_tag="VLF19_10190"
     CDS             7253..8401
                     /locus_tag="VLF19_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE93665.1"
                     /translation="MAVFHFALTELPPETDGLRAEVRDFLKTELAGRTSVRRARSWGG
                     WDPQFSRKVGARGWIGMTWPKRYGGHERTFLERYVVLEEMLAAGAPVGAHWVADRQSG
                     PLLLRFGTEAQRERFVPAIARGELAFAIGMSEPDSGSDLASIRTRAVRRDGGYVVNGT
                     KVWTSNAHRARYAIALFRTAVVPDKKHEGLTQFLVDLESPGITVRPIINLAGSHDFNE
                     VVFQDAFVPEDMRVGGEGDGWKQVTTELAFERSGPERYLSSIALVLELIREQERRPTE
                     RGAALVGRLVAHLHTLRQMSLSVAGMLQAGQNPNLEAALVKDVGTVFEQEVPEAAHAL
                     LDVEPTIDSGDMLEQTLGYLVQRAPSFSLRGGTREVLRGIIARGLGLR"
     gene            8398..9477
                     /locus_tag="VLF19_10195"
     CDS             8398..9477
                     /locus_tag="VLF19_10195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE93666.1"
                     /translation="MSAPAGDLRSLLGDTVTRLFGDLVTKEALESAEKGTWPEVLWQA
                     LEEGGLTLPLVPEARGGAGGTWLDAHVVVRAAGRHAAPVPLAETIVASWLLATAGLDV
                     PLGPLTIAPAQPDDRVRLRRAGGGWRLEGVAGRVPWGAAAGQVVVVAEADAGPVVALV
                     ARDAGRVTPDRNLALEPRDTLTYADAPVLAAAPLASAGAMTAGETVALYGALVRSAQM
                     AGALESLLQQSVRYATERRQFGRPIGNFQAIQHGLAVLAGHAAASGIAAERAFAAAER
                     GDCAFEVAAAKIRCGEAAGVCASIAHQTHGAIGFTYEHSLHFATRRLWSWRAEFGSES
                     RWASALGRRVIARGADALWADLTAR"
     gene            9719..10522
                     /locus_tag="VLF19_10200"
     CDS             9719..10522
                     /locus_tag="VLF19_10200"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93667.1"
                     /translation="METRPPKVVVSDVSVTFESHGRTVVALDRISLQVASGEFLCVVG
                     PSGSGKSTLLRVLAGLLRQRHGEVHIAADRPGTPLTAMVFQEHALLPWRTVLDNVAFG
                     LENRGVGRAEREARAREMLAMVGLARSGRHYPHQLSGGMKQRVGIARALANDPEVLLM
                     DEPLASLDAQTRTIMQEELLRIWATLGKTVVYVTHSLEESLLLGDRIVLLTARPGRVS
                     QVFPVDLGRPRGLEVRGSQAYGVLLEKIWSQLREEVVRAMDEDRGEGGA"
     gene            10637..11410
                     /locus_tag="VLF19_10205"
     CDS             10637..11410
                     /locus_tag="VLF19_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSE93668.1"
                     /translation="MARRLADHWIAVVSPVLLVIGWEWASAAGAVREAFFPRPTTIAR
                     HLWQLTAEGTLWEHTRITLVRIAWAFAVSALLGVATGLAMGLWRRLREGLDPVFAVVY
                     PIPSVLFLPLVSFLVPAGEVAVVVTTAVTSFFLVAFTTTHGVRQIDRLVLEAATHYGA
                     RGWRLFAAVLLPGALPFIFTGLRLGLGYALIVVVAVEIVAASRGLGAFLWLSWQVLKV
                     EDMYAAFLVIATLGAALSYGLAALRARLLPWVQDVASER"
     gene            11414..12157
                     /locus_tag="VLF19_10210"
     CDS             11414..12157
                     /locus_tag="VLF19_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSE93669.1"
                     /translation="MAATVPLAVLVLWEAASRLAILSPRYFPPPTVVVWGLAGSVTTG
                     DLGEHLVVTIARLAAAFAIAAVSGVPLGLLMGRVRPIRDAVDPYIAFLFPVPKIALLP
                     FLLILLGVGEPAFVLTGALSAFFQIVISTLAGVQTMDPRLLEVGHNYGARGARLFRKV
                     ILPAALPAIFTGLRLGLGLALVAIVAVEFIAAKSGLGHLVYRHWQMLSTTEMYGAFAL
                     VGALGLLLTRGLRALQGRMLTWQEDVGRL"
     gene            12380..13393
                     /locus_tag="VLF19_10215"
     CDS             12380..13393
                     /locus_tag="VLF19_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93670.1"
                     /translation="MKALRIVVLAALVLAAALAVTAGGTVAQAPPAVKIGYIPSDSFA
                     VLYIMADRYLTPAGINAQLVRLPSGAEITTQVATGQLQVGGAGMGAAGFNAVAAKLPI
                     EFVAPLHFGYLEDYFTVRKAVWGKEVKRIADLKGRHVALNTKGAAVEWMLDQVLRRDG
                     ITIKDVQVKIMPFPDMVPALESGAVDAAILTEPFPTLAEGKGVAVRPLQRPAGARAIP
                     ITTTFWNAEWARANPDLAHRVMVAYVKAARDLAADGGKGWHEDRNIDIMVKHTNAKRE
                     VIKQARPHVIDPNLQLDASTLDSMQKLNAELGYLKYKDFLSADRFFTFTYRDRALAEL
                     GRR"
     gene            complement(13448..13942)
                     /locus_tag="VLF19_10220"
     CDS             complement(13448..13942)
                     /locus_tag="VLF19_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012287190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSE93671.1"
                     /translation="MRSHRPLSLFLGTVLVLAAASASAQQTPPPYGPPITLEQAKKVM
                     AGAEAEAMKNKWNMVVTILDSGGNLVMSQRMDGTQFGSIEVAREKAYSAVAFRRPTKV
                     WQDLLAQGGANMRLLGLTGASLLDGGIPIVVDGKLIGGIGVSGATGEQDAQVARAGAA
                     ALAK"
     gene            complement(14165..>14498)
                     /locus_tag="VLF19_10225"
     CDS             complement(14165..>14498)
                     /locus_tag="VLF19_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93672.1"
                     /translation="ELRPVRFGPGLHVVSSNRDLDDDTMPEKKAVDAFAAALGGLPGE
                     AALADLLRTHDGERPICKHEERYGTVSSSIYIGRRVPRLLYADGAPCLNAFGDCSGAL
                     RAVSSGPA"
BASE COUNT         1852 a         5265 c         5185 g         2196 t
ORIGIN      
        1 gagcgcgacc gcctgcatgc gcaggcgcgc catctcctcc ggggttcgtt ccagagggta
       61 ggaatcgggg cgcagggtca tctccgcctg aacgagccca ccggacgcgc gcccatcgag
      121 gtgtgatata ccacgccttg ccgcgtcgcc ccgtcgtcgc gcagctcctg gccgaggccg
      181 tgctacagtc gcgggcgttc gagcgttcga ggcgcccgag gatctggacg gcgtcgtgga
      241 tggcgggccg gcgctcgccc gcgacatcgc gtcacccggg gagggattca tgacacagcg
      301 aggcttcgtg aagctgctga gcatcgcgct gctggcgctg ctcgcctggc ccgcgctcgc
      361 ggcggcgcag ggggtcaagg tcggcgccgt ggtgccgctc accggccgct atggcgccgg
      421 cggggcccag gtgcgggccg ggtacgagat cggcgtcgag cacgtcaacg cccggggcgg
      481 cgtgagcgtg ggcggcacga agatgccgct cgagctgacg atcctcgacg acgagtcgga
      541 cgccaccaag accgtggccc ggctggaaac cctcgccgcg cagggcgtgg tcgcctatct
      601 gggcggcttc ggctcggacc tgcacgcggc ggcagcatcg gtggccgaga agaacaagac
      661 gccctacctc ggcgtggcct tcgcgctcca caagatccac acgcagggct tccggtacct
      721 cttctcgccg ttctggaagt cgcccgacat caaggagcag acggtgaacc tgctgggcgg
      781 gctgcccgcc gcggagcgcc cgaagcgggc ggcgatcttc caggagaaga ccgactgggg
      841 ccgcgagatg gcgacggcct ggacggaggc ggccaaggcc gccggctacg cggtggtcgt
      901 caacggcgag tacgcgccgg gcgccaggga cttctcggac atcatcctca aggcgaaagg
      961 ggccaacgcc gacatcgtct tcgggctgcc gacgccgccc gacggcatga ccatcgtcaa
     1021 gcagatgaag gagctcggct tcagcccgaa gatgacgctc ctcatccgcg cgcccgacgc
     1081 gccggtgtgg tcgaagaacc tgggcaagga cggcgactac gtggtcctcg cccccggctg
     1141 gcaccacggc gtcaaggcgc ccggcgtgaa agagctgaac gaggcgcacc agaagaaggt
     1201 cgggcggccg gcggatccga tcgcgggacc ggcctatgcg tgcgtgcaga tcctcgccgc
     1261 cgcgatcgac aaggccggct cgctcgaccg cgacaagatc cgcgacgcca tcgccgcgac
     1321 gagcatgacc acggtcgtcg gacccgtgaa gttccggccc gacggcaccg gcgtcgtgaa
     1381 cgccgtgttc ctccagtggc tgaatggcaa gcaggagctg gtgtggccga aggacgcggc
     1441 caccgcggcg ctcgtctacc cggcgccgcc cttcgcgaag aggtgacgtc cggagccgcg
     1501 gggcggtgag gctgctcgag gtcgagcgcc tcacccgctc cttcggcggc gtccgcgcgg
     1561 tcagcgaggt caccttcgcc gtcgacgagg gcgaggtgat cggcgtcatg ggccccaacg
     1621 ggtcgggcaa aacgacgctc ttcaacctga tcacgggggc gctccggccg gacgccggag
     1681 cggtccgact caagggcacc gacatcaccg gctgggcccc gcaccgcgtg tgcgcggccg
     1741 gcatcgcgcg cacgttccag ctcgtccgcc cgttcccggg tctgaccgcc ctcgacaacg
     1801 tcctcgtggg ccggctctac ggccgcgagc gtggcgaccg gggctcggcc acggccgagg
     1861 ccgggcggct cctcgccctg gtcgggctcg acggcaaggc cggacggctg gccgcggagc
     1921 tgacgctcat ggaccgcaag cggctcgagc tggcgcgggc gctcgccacg agcccgcacc
     1981 tgctgctgct cgacgagctc atggccggcc tcaacccggc cgagacggag gcggcgatgg
     2041 cgctcatccg ccggctgcag tcggagggcg tcaccctgct catggtcgag cacatcgtgt
     2101 gggcgctcat ggagctgtca cagcgcctcg tcgtgctgag cgcgggtgag aaggtggcgg
     2161 agggctcgcc ggcgacggtg gccgcggacc ccacggtcat cgacatctac ctcggcagcg
     2221 ccgcggggag cgtgcggcgt gcttgaggtc gagggcgtcg aggcttcgta cgggccgttc
     2281 cgtgcgctcg cgggggtgac gctcacggtg cggccgggcg agatcgtggc gattctcgga
     2341 cccaacggcg ccggcaagac gaccctgatc cggtgcgtcg ccgggctcct gcgcccgcgc
     2401 gcgggcgtga tccgctggga gggggcgccg ctcaccggcg cgccggcgca cgccatcgtc
     2461 gagcgcggca tcgcggtcgt gcccgaagga cgccggctgt tcgcctcgat gacggtggag
     2521 gagcacctcg acctcggcgc cttcgcgccg cgcgcccgcg ccgagcgcgc gcggcggctg
     2581 gcggaggtct acgcgatctt cccggtgctg cgcgagcgcc gccgccaggt cgtgcaggca
     2641 ctgtccggtg gccagcagca gatggtggcc atcgggcgcg cgctcatggc ctctccacgc
     2701 ctgctcgcgc tcgacgagcc ttcgctcggg ctggcgccgc gtgtggtcga ctcgatcctc
     2761 ggcgtgctgg cgcagatcaa ccgcgccggc gtgtcgatcc ttctcgtcga gcagaacgtg
     2821 cacgccgcgc tggccctggc ccaccgcgcc tacatcctcg agtcgggccg cgtggccggt
     2881 gagggcgccg ccgccgcgct cctcgacgac ccgcacgtgc gccgcgccta cctcggccca
     2941 ctcgccgcgc gcgcatgacg ccggccgtcc tgctccagca gctcgtgctc ggcctgctca
     3001 tcggcgggct ctacgggctg gccgccgccg gtctgtccct cgtcttcggc gtgctcaagg
     3061 tcctcaacgt cgcccacggt gagctgatca tgctgggagg ctacggcgcc ttctggctgt
     3121 tcgcgctcgc cggcatcgac cccttcgtcg cgctcaccct cgtcgtgccc gcgtcgctgg
     3181 tcctgggcgc cctgctctac caggggctct tcgcctgggt cgtgcgcgcg caccacgagg
     3241 cgcgcatcaa gaactcgctg ctcatcggtt tcggcctcgg cctggcgctg cacgccgcgg
     3301 ccgtcctcct gtggacggcc gacgagcgct cggtgaccac ggactatggg ggccgggtca
     3361 tcgagctggg cggcatctat ctcccggtgg tgcgcctggc gagcctcgtg ctggccttcg
     3421 ccatcacggc cggcctccac ctgctgctcg ctcgctggcg gtggggccgc gccatccgcg
     3481 cgacggcgga cgactgggag gccgcagcga tgaccggcat cgacgtgcgc cgtgcctact
     3541 tgctggcctt cgcgctgggc accggcctcg ccggggcggc cggcacgctc gtgagcgtgg
     3601 gctactcgat cagcccctcg atcgggctcg agtggacgct gaaggcactg atcgtcgtcg
     3661 tgctggccgg gctcggctcc atgctcggca ccttcgtcgg cgggctcgtg ctcgggctcg
     3721 ccgaagccgt gagcgccacg ctgttcggcg gaccgtaccg cgaggtcgtc ggactgatca
     3781 tcttcttcgt ggtgctggtg gcccggcccc agggcctgtt ctcgcgatga ccggcgcgcg
     3841 gtggacgatg gtcggcgtgg cggccgtcgc ggcgctggcg tgggtgccgc tcgtcgtccg
     3901 ccgcgacgac gtggtgaact tcctcgtgct gacgctgctc accatcacgc tggcccagtc
     3961 gtggaacatc gtggccggct acgcggggca ggtgaacctg ggccacgcgg ccttcttcgg
     4021 cctcggcgcg ctggtgacgc gcacgctgtg gatcgcggac gtcgcggtgc cgctggcgat
     4081 ggcggccggc gccgccgtgg ccgtcgcctt cgcgctcgcc gtcggcgccg cggcgttccg
     4141 gctgcgcgga gcctacttcg ccatcggcac gctggcgctc ggggagatcc tccgcatcac
     4201 ggtcggcaac gtgctggccg agatctccac gctgccgacc acggcgatcg ccgggtaccg
     4261 gctcgccttt cgctactggc tcgcgctcgg tctggcagca ctttcggtgg ccgccgtcgc
     4321 cgtgctcgcc ggctcgcggc tgggcctggg catgcaggcg atccgcgagg acgaggaggc
     4381 ggcggaggcg acgggcgtcg gcgcgctggg tctcaagctg atcgcgctcg tgctctccac
     4441 ggcgctcgcg ggcctcgccg gcgggctctt cgcctactac cacatctcgt actacccctc
     4501 gcaccccttc agcccgcact ggacgttcga cgcgctgctc gtcaccttca tcgggggcgt
     4561 cggcaccctg cacgggccca tcctcggtgc ggtgttctac gtgttcctga aggagtacct
     4621 ggccatccgg tgggtggact ttcacctgct gatcttcggc gccctcttcg tggccatcgt
     4681 cctgttgctt ccggggggat tggtcgaggc gggcgcccgg gtccgtcgtc tgcgcgcgtg
     4741 atcccgtcgg cgagacgctg tgcgcggccc aaggtcatcc catcgggttc agtctcacgg
     4801 gcgcctggct ggacgacact tggtcccatt gctcctgcag tcatccggcg tcaggatggc
     4861 tgcctgggga gacacgggct ggcgttcatc ggcatcgcac ttgctggttg atcgagcatg
     4921 aacaccctgg tcacccagtt ttcgcccatc gagaccagcg acggcggtgt ctactcggcg
     4981 caagcgtgcg gcgtccagcg cgacgacggg ctgtgggaag gctggctcgc cttcttcgac
     5041 gcccatgggc gggcgtggag agcgggacgg gaaaccgtgc agccctccca ggcggccctc
     5101 gaatactggg cctcggggct gacgcccgtg tacctcgagg gcgcattgaa tcgggcggtg
     5161 cgggtggccc cggtggccgc ggtcacgccg gagaccgact gaccgtcgag aactcgacgc
     5221 tcgaccgcgt agccgccgca cggggcgtga cggcgacgac gctggtcagc ggttgaggcg
     5281 aggagcgcca tgcgctggac gatgggcagg cggagcgaga acgtcgagga ccgccgggga
     5341 cgcggcctgc cactcgtcgc gggcggcggg atcggtacga tcgtcatcct gctgctcgcg
     5401 ctattcttcg gcctcgaccc cggagtcctg ctgcagaccg gcgcgccgcc gtcggaccgg
     5461 acgcaggcgc cggaggccac gggcggccac gacgagatgc gcgacttcgt ctcggtggtc
     5521 cttgccgaca ccgaggacac gtggcgcacc ctgttccgga gcatgaaccg cgagtaccgc
     5581 gacccgacgc tggtgctctt caccggccgg gtccagtccg cgtgcgggat cgccggcgcg
     5641 gcggtggggc cgttctactg ccccggcgac cacaagctct acctcgacct cgccttcttc
     5701 cgcgagctcc gcgaccgctt cggcgcgccg ggcgacttcg cgcgggccta cgtgatcgcc
     5761 cacgaggtcg gccaccacgt gcagaccctc ctgggcatct ccgagcgggt catggccaca
     5821 cgccagcgcg tgggcgagac cgaggccaac gccctctcgg tacggctgga gctgcaggcg
     5881 gactgcttcg cgggcgtctg ggcgcaccac gccgatcgag cgcgccaggt cctcgaggcc
     5941 ggcgacctgg aagaggccct ggccgccgcg gcggcgatcg gtgacgaccg gctacagcgc
     6001 cagacccagg gccacgtggt gcccgagtcg tttacccatg gcacctcggg ccagcgcgtc
     6061 acctggttcc ggcgcggctt cgactccggc gacgtccgcc ggtgcgacac attcacggcc
     6121 ggcggctccg ggccggagcg gcccacgagg cgctgaaccg gcagcccgga actcctccat
     6181 ggtgagcgtc ccgccgggcg cttgcccggc tgccggggga ccgactatca tccgtctcgt
     6241 gctcgacggc gccgccagat cccagccgtg aatccgcgct ccttcgccct ggcgcccctg
     6301 tggctcaggc tggtcctgct ccccctctcg ctgctcgccg cggcggcggc accggcgctc
     6361 gccgcggatg ccccagacgt cgccagcgcc cccgacgtcg ccaccgcccc gtcgtcgtcg
     6421 acggcctgac gctcttccgc gtccgaggca tatccgcctt ccccgcccac gagcgggcgc
     6481 gcgtcatcgc cgagcgagtc cgcgcggttg ccgccgattc cgccgtggcg acgagcaccc
     6541 tgcgcgtcgt ccctgccgag cgctccagcg acatcgtgat gggggaccac cccatcatgt
     6601 ccgtgttcga cgccgacgcc gagatcgaga ggttgaaccg gcaggacctc gcccggagct
     6661 acgccaaccg catccgcgtc gtcgtcgagg cgtattgccg cgatcgctcg ccccgcagcc
     6721 ttctcgtcgg cgcggcggcg agcctcgcgg ccaccgcggc gctcgtgggc acggtgctcc
     6781 tcctgctccg gttctggcgg cgcctcagcg tggtcctgga gccgcgttac acgcggcgcc
     6841 tgcgctcgct cgagatccag acaccgcact gcaccggaac gtccaggacg tgttcaacga
     6901 gtacggcgtg cagatcatga cgccggccta cgagtcggac ccggcgagcc cgaaggtggt
     6961 gccgagggat cggtggtgtc cgccgcccgc gcgagcgctg gaggagcctc cccggagcgc
     7021 ggccaattga ccggtcctcc tggacccatc cctgtgctgc ttctgcgagc agcgtctcag
     7081 agcgcccgtg catcctcatc gcggtcctcc ggtgcgggtt gctggtggga ggcgctggct
     7141 ccccgactca ctcgaggacg aacaccgttg agacgcgcac tcgggcgggt ccaagggtaa
     7201 gggtctcgcc gggaccgtcg agaacgcggt acactcccgt tcaccgccgg ccatggccgt
     7261 cttccacttc gcactgaccg agctgccgcc cgagaccgac gggctccgcg ccgaggtccg
     7321 cgacttcctg aagaccgagc tcgccgggcg gacgagcgtc cgccgcgcgc ggtcgtgggg
     7381 cggatgggac ccgcagttca gccgcaaggt cggcgcgcgc ggctggatcg gcatgacctg
     7441 gcccaagcgc tacgggggcc acgagcgcac cttcctcgag cgttacgtcg tgctggagga
     7501 gatgctcgcc gccggcgcgc ccgtgggcgc ccactgggtg gcagaccgcc agagcggccc
     7561 gctgctgctc cgcttcggca ccgaggccca gcgcgagcgc ttcgtgcccg ccatcgcgcg
     7621 gggtgagctg gccttcgcca tcggcatgag cgagccggac tccggctcgg acctggcctc
     7681 catccggacg cgcgcggtgc gccgcgacgg gggctacgtc gtcaacggca ccaaggtgtg
     7741 gacctcgaac gcccatcgcg cccggtacgc gatcgcgctg ttccgcacgg ccgtggtgcc
     7801 ggacaagaag cacgaaggcc tcacgcagtt cctggtggat ctcgagtccc cgggcatcac
     7861 cgtccgcccg atcatcaacc tcgccggctc gcacgacttc aacgaggtgg tcttccagga
     7921 cgccttcgta cccgaggaca tgcgcgtggg cggcgagggc gacggctgga agcaggtgac
     7981 gaccgagctc gccttcgagc gcagcgggcc cgagcgctat ctgtcctcga tcgccctcgt
     8041 gctcgagctg atccgcgagc aggagcgccg gccgacggag cgcggcgccg ccctggtcgg
     8101 ccgcctcgtc gcgcacctgc acacgctccg gcagatgtcg ctctccgtcg ccggcatgct
     8161 gcaggcaggc cagaacccga acctcgaggc ggcgctggtg aaggacgtgg gcacggtctt
     8221 cgagcaggag gtccccgagg ccgcccacgc gctgctcgac gtcgagccca cgatcgacag
     8281 tggcgacatg ctcgagcaga cgctcggcta cctggtgcag cgcgcgccgt cgttctcgct
     8341 ccggggcggg acgcgcgagg tgctgcgcgg catcatcgcg cgagggctcg gtctccgatg
     8401 agcgcgccgg ctggcgacct tcgctcgctg ctcggcgaca ccgtcacgcg gctcttcgga
     8461 gacctcgtga ccaaggaggc gctggagtcg gcagagaagg gcacgtggcc ggaggtgctc
     8521 tggcaggcgc tggaggaggg cgggctgacc ctgccgctcg tccccgaggc gcgtggcggc
     8581 gcgggcggga cgtggctcga cgcgcacgtg gtcgtgcgcg cggccggccg gcacgctgcg
     8641 ccggtgccgc tcgccgagac catcgtggcc tcgtggctgc tcgccaccgc cggcctcgac
     8701 gtgccgctcg ggccgctcac gatcgcgccc gcgcagccgg acgaccgcgt gcgtctccgg
     8761 cgcgccggcg gcggctggcg gctcgagggt gtcgccggtc gcgtgccgtg gggcgcagcc
     8821 gcgggccagg tcgtggtggt ggccgaggcg gacgccggcc cggtggtggc gctcgtcgcg
     8881 cgggacgccg gacgggtcac acccgaccga aatctcgcgc tcgagccgcg cgacacgctc
     8941 acgtatgcgg acgcgcccgt cctggccgcg gcgccgctcg caagcgccgg cgccatgacg
     9001 gccggtgaga ccgttgcgct ctacggcgcg ctcgtgcgct cggcgcagat ggccggcgcg
     9061 ctcgagagtc tgctccagca gtcggtccgc tacgccaccg agcgccggca gttcggtcgc
     9121 ccgatcggca acttccaggc gatccagcac ggcctcgccg tgctcgccgg ccacgccgcc
     9181 gcctccggga tcgccgcgga gcgcgcgttc gccgcggcgg agcgcggcga ctgcgctttc
     9241 gaggtcgccg ccgccaagat ccgctgtggc gaggccgccg gcgtgtgcgc ctcgatcgcc
     9301 caccagacgc acggcgccat cggcttcacc tacgagcact cgctgcactt cgcgacccgc
     9361 cggctctggt cgtggcgcgc cgagttcggc agcgagagcc gctgggcgag cgcactaggg
     9421 cggcgggtga tcgcgcgcgg tgccgacgcg ctctgggccg acctcactgc ccgctgaggg
     9481 gaagccacca attcgatcac ccgccgatgc cgggtggcgg cgaatgcccg ccccggccgg
     9541 cgggtagata ctgcgggact ccacgggcaa ccctgccgcg tggcctcgcc gatacccctg
     9601 aaccggctcg agtcggttct tcggcaaggg gcaggcgcgc cccgtcgggc ttgaaccgcc
     9661 ggcggaggtc gggcagaatc cccgcgcacc ggcgcgccgg cttgcgccct gccgagtgat
     9721 ggagacgcgc ccccccaagg tcgtcgtcag cgacgtcagc gtgacgttcg agagccacgg
     9781 ccgcaccgtc gtggcactcg atcgcatctc gctccaggtg gcctccgggg agttcctctg
     9841 cgtcgtcgga cccagcggca gcggcaagtc cacgctcctc cgcgtcctgg ccggcctgct
     9901 gcggcagcgt cacggtgaag tgcacatcgc ggccgaccgg cccgggactc cgctgacggc
     9961 catggtgttc caggagcacg ccctgctgcc ctggcgcacc gtcctcgaca acgtggcgtt
    10021 cggtctcgag aatcgcggtg tcgggcgggc cgagcgggag gcgcgagccc gcgaaatgct
    10081 cgcgatggtc ggcctggcgc gctccgggcg ccactatcca catcagctct ccggaggcat
    10141 gaagcagcgc gtgggcatcg cccgcgcgct ggcgaacgat cccgaggtcc tcctgatgga
    10201 cgagcccctc gcctcgctcg acgcccagac gcggacgatc atgcaggagg agctgctccg
    10261 catctgggcc acactgggca agacggtcgt gtacgtgacc cactcgctgg aggaatcgct
    10321 gctcctcggt gaccgcatcg tgctcctcac ggcgcgaccg gggcgggtga gccaggtctt
    10381 ccccgtggat ctcgggcggc cgcgcgggct cgaggtgcgc gggtcgcagg cgtacggcgt
    10441 gctactcgaa aagatctggt cgcagctccg cgaggaggtc gtgcgggcga tggacgagga
    10501 ccgtggcgag ggcggtgcgt gagcgctttg acccgtggcc ggatcgtcaa tctgacgacg
    10561 gcgaaggccc tggggatcgc gatcccgccc gcatggccga tccgcaccga ccgcgtgatg
    10621 tgagcgtgtg attgccgtgg cgcgtcggct ggcggaccac tggatcgccg tcgtctcgcc
    10681 cgtgctcctg gtcatcggct gggaatgggc gagcgcggcc ggtgcggtgc gcgaggcgtt
    10741 cttcccgcgg ccgacgacga tcgcccgcca tctctggcag ctcaccgcgg agggcacgct
    10801 gtgggagcac acgcggatca cgttggtccg gatcgcgtgg gcgttcgccg tctccgccct
    10861 cctcggcgtg gcgaccggtc tcgcgatggg attgtggcga aggctccgcg agggcctcga
    10921 cccggtcttc gccgtcgtct atccgatccc gagcgtgctg ttcctgccgc tggtgtcctt
    10981 cctggtcccg gcgggtgagg tggcggtcgt ggtgacgacc gcggtcacct cgttcttcct
    11041 cgtcgcattc acgacgacgc acggcgtgag acagatcgac cggctggtgc tcgaggcggc
    11101 gacgcactac ggtgcgcgcg gctggcggct cttcgccgcg gtgctgctgc cgggcgcgct
    11161 gccgttcatc ttcacggggc tgcggctcgg cctgggctac gcgctgatcg tcgtcgtggc
    11221 cgtcgagatc gtcgcggcga gccgtgggct cggggcgttc ctgtggctct cgtggcaggt
    11281 gctgaaggtc gaggacatgt acgcggcgtt cctcgtcatc gccaccctcg gcgccgcgct
    11341 ctcctacggg ctggcggccc tccgcgcgcg gctgctgccg tgggtgcagg acgtggcgtc
    11401 ggagcgctga gcagtggccg ccaccgttcc gctcgccgtg ctcgtgctgt gggaggccgc
    11461 ctcgcgcctg gccatcctct ctccgcgcta cttccccccg ccgaccgtgg tggtctgggg
    11521 cctcgccggc agcgtgacga cgggcgacct cggcgagcac ctcgtcgtca cgatcgcgcg
    11581 cctggcagcg gcgttcgcca tcgccgcggt gtcgggcgtc cccctgggcc tcctcatggg
    11641 ccgcgtgcgg ccgatccgcg atgccgtcga tccctacatc gccttcctgt tcccggtgcc
    11701 gaagatcgcg ctgctgcctt tcctcctgat cctgctgggc gtgggggagc cggccttcgt
    11761 cctgaccggc gcgctctcgg ccttcttcca gatcgtcatc agcacgctcg cgggcgtgca
    11821 gacgatggac ccgcgcctgc tcgaggtcgg gcataactac ggcgcgcgcg gcgcgcggct
    11881 gttccgcaag gtcatcctgc ccgccgcgct gccggcgatc ttcacgggcc tgcggctggg
    11941 cctcggcctg gcgctggtcg ccatcgtcgc ggtagagttc atcgccgcca agtccggtct
    12001 gggccacctc gtgtaccggc actggcagat gctcagcacg accgagatgt acggcgcgtt
    12061 cgccctcgtc ggggcgctcg ggctcctgct cacgcggggc ctgcgggcgc tgcagggaag
    12121 gatgctcacg tggcaggaag atgtcggcag actatgaaca tggggccgcg acatgaacat
    12181 gggggccccg acatggcccc caagcccccg ggtggctcgg cacgccccgg cggagccgtg
    12241 gcgcgcctcg atcaggcgcg caggggctcg acgcttcggg ggcccgacat ggccccccag
    12301 cccccgggtg gctcggcacg ccccggcgga gccgtggcgc gtctctatgc ccgagtagct
    12361 tagaccagag gagacccgca tgaaggcact ccgcatcgtc gtgctcgcag cgctcgttct
    12421 ggcggccgcg ctggccgtga cggccggcgg caccgtcgcc caggcgccgc cggccgtgaa
    12481 gatcggctac atcccgtccg actctttcgc ggtgctctac atcatggccg accgctacct
    12541 gacgcccgcg ggcatcaacg cacagctcgt ccggcttccc agcggcgccg agatcaccac
    12601 ccaggtcgcc accgggcaac tccaggtcgg cggcgcgggc atgggcgccg ccggcttcaa
    12661 cgcggtcgcg gccaagctgc cgatagagtt cgtcgcgccg ctgcacttcg gttacctcga
    12721 agactacttc accgtgcgca aggccgtatg gggcaaggag gtcaagcgta tcgccgacct
    12781 caagggacgg cacgtggcgc tcaacacgaa gggcgcggcc gtcgagtgga tgctcgacca
    12841 ggtgctccgc cgcgacggca tcacgatcaa ggacgtccag gtgaagatca tgccgttccc
    12901 cgacatggtg cccgcgctgg agtcgggcgc cgtcgacgcc gccattctca ccgagccctt
    12961 cccgacgctc gccgagggaa agggcgtggc cgtgcggccg ctccagcgcc ccgccggcgc
    13021 cagagccatc ccgatcacga ccacgttctg gaacgcggag tgggccaggg ccaaccccga
    13081 cctcgcccac agggtgatgg tggcctacgt caaggcggcg cgcgacctcg ccgcggacgg
    13141 cggcaagggc tggcacgagg accgcaacat cgacatcatg gtgaagcaca cgaacgccaa
    13201 gcgcgaggtg atcaagcagg cgcggcccca tgtgatcgac ccgaacctcc agctcgacgc
    13261 gagcacgctc gacagcatgc agaagctgaa cgcggagctg ggctacctga agtacaagga
    13321 cttcctctcc gccgaccggt tcttcacctt cacctaccgc gatcgcgccc tggcggagct
    13381 gggccggcgc tgagccgcgc cggcgggaga ccggcggctg gccgccggtc tcccgcgcgt
    13441 aacgtgacta cttcgccagg gccgcggcgc ccgcccgtgc gacctgggcg tcctgctccc
    13501 cggtcgcgcc ggacacccct atgccgccga tcagcttgcc gtccacgacg atcgggatgc
    13561 cgccgtccag gaggctggcg ccggtgaggc cgagcaggcg catgttggcg ccaccctgcg
    13621 cgaggaggtc ctgccacacc ttggtcggcc gacggaaggc gacggccgag tacgccttct
    13681 ctctcgccac ctcgatgctt ccgaactggg tgccgtccat gcgctggctc atgacgaggt
    13741 tgccgcccga gtccaggatg gtgaccacca tgttccactt gttcttcatg gcctcggcct
    13801 ccgcccccgc catcaccttc ttcgcctgct ccagcgtgat ggggggaccg tagggcggcg
    13861 gcgtctgctg cgcgctcgcc gacgccgccg cgaggaccag caccgtgccg aggaacaggg
    13921 acagcggtcg gtgacttctc atacgctcct ccttgtggac ggacgccgag tcggagagcc
    13981 cgacagacaa accgccgtca ccctccgtgg ttcccgtgcc gcgacctccc gtgtcgtctt
    14041 cacggcggca cggccctgca cacacccggc cccgactgcg cccgcctgcc acggtgtggg
    14101 cgagcggggc ccggattcgc atcgtcgcgc accgttcccg gttgctcaca tctgcccccg
    14161 agattcatgc cgggccggag ctcacggcgc gcagggcccc ggagcagtcg ccgaaggcat
    14221 tcaggcaggg cgccccgtcg gcatagagca ggcgcggcac ccgacgcccg atgtagatcg
    14281 acgacgacac cgtgccgtag cgctcttcgt gcttgcagat cggccgctcg ccgtcgtgtg
    14341 tgcgcaggag gtccgccagc gcggcctcgc cgggcaaccc gccgagcgcg gcggcgaagg
    14401 cgtcgacggc cttcttctcg ggcatcgtgt cgtcgtcgag gtcccggttg ctggagacga
    14461 cgtgcaggcc gggcccgaag cgcaccggtc gaagctcg
//
LOCUS       DATIAZ010000291         3561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_213843, whole genome shotgun sequence.
ACCESSION   DATIAZ010000291 DATIAZ010000000
VERSION     DATIAZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213843"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VLF19_10230"
     CDS             <1..1232
                     /locus_tag="VLF19_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSE93673.1"
                     /translation="TVPEELIGFLRGKRQVLVLEEGMPNYIEQDVKALAHDARLDVAI
                     RGKDLLSPHGEYVPQLVITGLRRFLAGAATRSVGVSMLEERYRALFGHQERVRALLPQ
                     PVAKRPPTFCTGCPERPVFSAMKILRTREPMLGDTHVAHDIGCSTFSTQAPFNVGNSV
                     LGYGMGLASSNAVAPMFGKRVISVMGDGGFWHNGLGNGVANAVVNKQDSVLVILDNGF
                     AAATGQHHIPSTGTNARREPTGMTIPEALRGVGVKWIRTVNSYRIADVMRALREALTT
                     REGGLKVVIAKGECQLERQRRVRAASRQAVAAGRQVVQPRFGVDPDVCTGDHSCMRVN
                     GCPSLTLRESPDPLREDPIAHVDQTCVGCGVCGEVAHAAVLCPSFYEMRIVRNPARWR
                     RWRARLRTAVIGRLAPA"
     gene            1345..2883
                     /locus_tag="VLF19_10235"
     CDS             1345..2883
                     /locus_tag="VLF19_10235"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HSE93674.1"
                     /translation="MTIDTLISILIPAVGGQGGGVLAEWIVDAATIDGVTAHSTSIPG
                     VAQRTGSTTYYVELFTGPAADPPAFSLYPVPGALDVLLAPEFLEVGRALEAGMPSPGR
                     TTIVASTHRLYSIHEKMATGRSIYPVAQLEAAARSLSRRFIGFDALAVAREHRTEVNA
                     VLLGALAASGAVPVRAAAFRTSIERKGVQVESNRRGFDAGLAFAREGARPPAEEAAKR
                     PPRIPGTFAQELAVFPETLRPLVGEALGRLVDYQDERYARLYLERLASVMGAGGDPAL
                     TAIVARHLALWMTYEDAIRVADLKTRAGRFARIRAEAGADDAEIVVTDYLKPDLDELY
                     GVLPYRLVAPFARWAERRWPHGRPTMGQHVRTTTVLGFLRLWLLARCRRLRPISYRAR
                     REHEGMEHWLATVRRCAQWSGELACEVARAAQLVKGYGDVRRRLTGLLDHLLQTVLQA
                     AALEAVRQAGVPATTQLAATYRRLVLQGPDAEARAVTLASDVLAHLQRNEPDAALATL
                     KSAT"
     gene            complement(3240..>3561)
                     /locus_tag="VLF19_10240"
     CDS             complement(3240..>3561)
                     /locus_tag="VLF19_10240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSE93675.1"
                     /translation="VGKSPERRRGDRVRVTFPVRVSTYDGGEWEGWAFDLSPFGMRVR
                     GDVPTGRGSAAKLSFDLPDGGLPLRVMSLLVRTDETGHAFYFVNLTAEDFERLGAFTR
                     QQIH"
BASE COUNT          483 a         1289 c         1291 g          498 t
ORIGIN      
        1 cgacggtgcc ggaagagctg atcggcttcc tgcgcggcaa gcggcaggtg ctggtgctcg
       61 aggaggggat gccgaactac atcgagcagg atgtcaaggc gctcgcccac gacgcccggc
      121 tcgacgtggc gatccgcggc aaggacctcc tgtcgccaca cggcgagtac gtcccgcagc
      181 tcgtgatcac cggtctccgg cggttcctcg ccggtgctgc gacgcgatcg gtcggcgtgt
      241 cgatgctcga ggaacgctac cgggcgctct tcggccacca ggagcgcgtg cgggcgctgc
      301 tgccgcagcc ggtggccaag cgcccgccga cgttctgcac ggggtgcccg gagcggcccg
      361 tgttcagcgc catgaagatc ctgaggacgc gcgagccgat gctgggcgac acgcacgtgg
      421 ctcacgacat cggctgcagc acgttctcca cccaggcgcc gttcaacgtg ggcaactccg
      481 tcctcggcta cggcatgggc ctggcgtcct ccaacgcggt ggccccgatg ttcggcaagc
      541 gcgtgatctc ggtcatgggc gacggcggct tctggcacaa cgggctcggc aacggcgtgg
      601 ccaatgccgt cgtgaacaag caggattcgg tgctcgtcat cctcgacaat ggctttgcgg
      661 ccgccaccgg ccagcaccac atcccgtcca cgggcacgaa cgctcgccgg gagcccaccg
      721 gcatgacgat ccccgaggcg ctccggggcg tgggcgtgaa atggatccgc acggtgaact
      781 cgtaccgcat cgccgacgtc atgcgggcgc tccgcgaggc gctcacgacg cgtgagggcg
      841 ggctgaaggt cgtcatcgcc aagggcgagt gccagctcga gcgccagcgg cgcgtgcgcg
      901 cggcctcgcg gcaggcggtg gcggcggggc ggcaggtggt ccagccgcgc ttcggcgtcg
      961 atccggacgt ctgcacgggg gaccactcct gcatgcgcgt caacggctgc ccgtcgctga
     1021 cgctgcgcga gagccccgac ccgctgcgcg aggacccgat cgcccacgtc gaccagacgt
     1081 gcgtcgggtg cggggtgtgc ggcgaggtgg cgcacgccgc cgtgctctgc ccctcgttct
     1141 acgagatgcg catcgtgcgg aaccccgcgc gctggcgccg gtggcgcgct cggctgcgca
     1201 cggccgtgat cgggcggctc gccccggcgt gaccgaaccg atgctcgcgc gccatccagc
     1261 ccttgcgccc gaccgggcgc cggaggtgcc ggcggggtcg acccggggag aggcggcggg
     1321 agccgtcgag acccccactg catcgtgacc atcgacacgc tcatctcgat cctgatcccc
     1381 gcggtcggcg gtcagggcgg cggcgtcctg gccgagtgga tcgtggacgc ggcgacgatc
     1441 gatggtgtca ctgcgcactc gacctcgatc cccggcgtcg cgcagcgcac gggctccacc
     1501 acgtactacg tcgagctctt caccggtccg gccgccgacc cgcccgcctt ctcgctctac
     1561 ccggtgcccg gcgccctcga cgtgctgctc gcgcccgagt tcctggaggt cgggcgggcg
     1621 ctcgaggccg gcatgccgtc gcccggccgc acgacgatcg tcgcgagcac ccaccgcctc
     1681 tactcgatcc acgagaagat ggccaccgga cggtccatct acccggtggc gcagctcgag
     1741 gcagccgcgc ggtcgctctc gcgccgcttc atcggcttcg acgcgctcgc cgtggcgcgg
     1801 gagcaccgca ccgaggtcaa cgcggtgctg ctgggcgcgc tggcggcctc gggtgcagtg
     1861 ccggtgcggg cggcggcgtt ccggacctcc atcgagcgca agggcgtgca ggtcgagagc
     1921 aaccggcgag gcttcgatgc cggcctggcg ttcgccaggg agggtgcgcg gccgcccgcg
     1981 gaggaggccg cgaagcgacc gccgcggatc ccggggacgt tcgcgcagga gctcgcggtc
     2041 tttcccgaga cgcttcgtcc gctcgtcggc gaggcgctgg gccggctcgt cgactaccag
     2101 gacgagcgct acgccaggct gtacctcgag cggctcgcgt cggtgatggg cgccggcggc
     2161 gatccggcgc tgaccgccat cgtcgcgcgc cacctggcgc tctggatgac gtacgaggac
     2221 gcgatccggg tagccgacct caagacccgc gccggccggt tcgcccgcat tcgcgccgag
     2281 gccggcgcgg acgacgcgga aatcgtggtc accgactatc tcaagcccga tctcgacgag
     2341 ctgtacggcg tgctgccgta ccggctcgtc gccccgttcg cgcgctgggc cgagcggcgc
     2401 tggccacacg gtcggccgac catgggccag cacgtgcgca cgacgaccgt gctcggcttc
     2461 ctccgcctct ggctgctggc gcgatgccgg cggctcaggc ccatctccta ccgcgcccgg
     2521 cgcgagcacg agggcatgga gcactggctg gcgacggtca ggcgctgcgc ccagtggagc
     2581 ggggagctcg cgtgcgaggt cgcgcgcgcg gcccagctcg tgaagggtta cggagacgtg
     2641 cggcggcgcc tgacgggtct gctcgatcat ctgcttcaga ccgtgctcca ggccgccgcg
     2701 ctcgaggccg tccgccaggc cggcgtgcca gccacgacgc agctcgccgc cacgtaccgc
     2761 aggctcgtgc tccagggacc agacgccgag gcgcgtgcgg tgacgctcgc ctccgacgtc
     2821 ctcgcccacc tccagcgcaa cgagcccgac gccgccctcg ccaccctcaa gtccgcgacg
     2881 tagcatccgc ggagcccgcg ttccgctgga acggggcgca cccgggcggc acaggactac
     2941 ctcgccgcag gagcttgtgg gttcccgtca ggctcgaccg gacgctgggg gtgtgctcca
     3001 agcccccatg ttcatgagac tcctgcgcgt gggaccgagg cgcgccgcgg cgccgccgag
     3061 gcgcgcctca gcgggtggga ctttggcgga gccatccggg gccacgcacg ttggtaagaa
     3121 ccgtgcgggt gatcgaggcg tgttccggcg tgccgagcgt tcgggctttg gggcccgctc
     3181 gggctcccaa cagatgagcc gagttggggg gcttgggggg cccgctcggg gccccccatc
     3241 taatggatct gctgacgagt gaaagcgccc agccgctcga agtcttcggc ggtgagattg
     3301 acgaagtaga acgcgtgacc ggtctcgtcg gtccggacga gcagggacat cacccgcagc
     3361 ggcaggccgc cgtcgggcag atcgaacgag agcttggcgg ccgaccctcg cccggtgggc
     3421 acgtcgccgc gcactcgcat cccgaacgga ctcaaatcga aggcccagcc ctcccactcg
     3481 ccgccgtcgt aggtggacac gcggaccggg aaggtgacac gcacgcggtc gccgcgcctc
     3541 cgctccgggc tcttgcccac g
//
LOCUS       DATIAZ010000292         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_479211, whole genome shotgun sequence.
ACCESSION   DATIAZ010000292 DATIAZ010000000
VERSION     DATIAZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479211"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..200)
                     /locus_tag="VLF19_10245"
                     /pseudo
     CDS             complement(1..200)
                     /locus_tag="VLF19_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(471..1211)
                     /gene="istB"
                     /locus_tag="VLF19_10250"
     CDS             complement(471..1211)
                     /gene="istB"
                     /locus_tag="VLF19_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HSE93676.1"
                     /translation="MLVHPTLTQLTELGLAGMAKAFKELRDHPQAQALEHAEWLALLL
                     DREATERKDRRLKTRLRAARLRFVQACAEDVDFTATRGLDRRLFQELTTCQWLRGHHN
                     LIITGACGTGKTWLACALGHNAARADLAVLYHRLPRLFAELALARGDGRYPRLFRSLC
                     RVHLLILDDWGPEPMTAEQRRDLLEIIEDRYGRASTLVTSQIPVSRWHDVVGEPTLAD
                     AILDRLVHNAHRIELEGDSLRKRKANRS"
     gene            complement(1233..2762)
                     /gene="istA"
                     /locus_tag="VLF19_10255"
     CDS             complement(1233..2762)
                     /gene="istA"
                     /locus_tag="VLF19_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058167704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSE93677.1"
                     /translation="MRQVGEVLRLKHACGMSDRVIARSLGLGRTTVGEYLRRASVAAL
                     GWPLPAGLSDADLERLLFPAEAARAPHRPQPNWAAVHAELKRPGVTLRLLWEEYRGAH
                     PDGYSLSRFCELSQAWRGHLSPVMRQTHRAGEKMFVDFAGQTVEVTDLASGEVRAAQI
                     FVAVLGASNFTYAEATWSQTLPDWIGAHGRALTFFGGVPKIIVPDNLKAGVSKACFFE
                     PGLNRTYADMAAHYGAAIVPARPRKPRDKAKVEVAVQLVERWILARLRHRRFFSLADL
                     NHAIRTLVDELDRKPSRHLGASRRALFQQIDQPALRPLPAEPYEYAEWKECRVGIDYH
                     VEIDKHYYSVPHGLIRQPITARITTKTVELFHRGKRVAAHLRAYAPRRHTTVAEHMPS
                     AHRRYAGWTQERLRRDAADIGPDTALLIETILEKKPHPEQGFRACLGIVRLLKSYGRE
                     RLEAACSRALAIGALSYTSVASILRNHLDRRHKPAPNPSGAADGPVLEHANLRGPDYF
                     H"
     gene            complement(3012..>3297)
                     /locus_tag="VLF19_10260"
     CDS             complement(3012..>3297)
                     /locus_tag="VLF19_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HSE93678.1"
                     /translation="ELLRMPPVVASTVALAMAIGFNYYFQRRITFRSNAKHYVAAPRF
                     VGITLCTLGANAVIFGALVSYIPYLPAQVITLGIIFPINYYLNKRITFRS"
BASE COUNT          515 a         1009 c         1258 g          515 t
ORIGIN      
        1 tgcgggtcga cgccgttgag cttggcggtg ccgatcaacg agtccatcac cgcggcgcgc
       61 tcaccgcccc gatcggagcc gcagaacagc cagcacttgc ggccaagggc gatgccgcgc
      121 agcgcccgct cggcggcagt gttgctgatc cagatccggc cagcctcgag caagcgggtg
      181 catgcgggcc agcgcttgag tgcgcattcc gatgaaggcg gccgctgaat ccggctgaag
      241 ccggacaccc aatccgatcg aagccggcca cggcatccga cgaagctgac cgggacggac
      301 ggcttgccgc aggcggttga gagagtcgct ggtgttcgcg gtctcgtcaa gcgcggcgca
      361 gctcgtcccc gtagcccaca gagggcaccg cgcgcgccgg tgcgtgcccc tccaagaggc
      421 tgacgcggcg gcgcgcgcgg gggatagctg tgggcccacg ggggcgagcg tcatgaccga
      481 ttggccttgc gcttgcgcag ggagtcgccc tcgagctcga tccgatgcgc gttgtgcacg
      541 aggcgatcga ggatggcgtc ggccagggtc ggctcgccga ccacgtcgtg ccagcggctg
      601 accgggatct ggctggtgac cagggtcgag gcgcggccgt agcggtcctc gatgatctcg
      661 aggagatcgc ggcgctgctc ggcggtcatg ggctccggcc cccagtcgtc gaggatcaac
      721 aggtggacgc ggcagaggct gcggaacagg cgcgggtagc ggccgtcgcc acgggcgaga
      781 gcgagctcgg cgaacagccg cggcagacga tgatagagga cggcgaggtc cgcgcgcgcc
      841 gcgttgtggc cgagcgcgca cgccagccag gtcttgccgg tgccgcaggc accggtgatg
      901 atcagattgt gatggcccct gagccactgg caggtggtga gctcctggaa gaggcgccga
      961 tcgaggccgc gggtggcggt gaagtcgacg tcctcggcgc aggcctggac gaagcgcagc
     1021 ctggcggcgc gcagccgggt cttgaggcgg cgatccttgc gctccgtggc ttcgcgatcg
     1081 agcaggaggg cgagccactc ggcgtgctcg agggcttgag cctggggatg gtcgcggagc
     1141 tccttgaagg ccttggccat gccggcgagg ccgagctcgg tgagctgggt caaggtggga
     1201 tggacgagca tgatctcctc gctgccaaag gttcagtgga agtagtcggg gccgcggagg
     1261 ttggcgtgct cgagcacggg cccgtcggcg gccccactgg ggttgggggc gggcttgtgg
     1321 cggcggtcga ggtggttcct gaggatcgag gcgacggaag tgtaggagag cgcgccgatc
     1381 gcgagggcac ggctgcaggc ggcttcgagc cgctcgcggc cgtaggactt gaggagacgg
     1441 acgatgccga ggcaggcgcg gaaaccttgc tcgggatggg gcttcttctc gaggatggtc
     1501 tcgatcagga gcgccgtgtc gggcccgatg tcggcggcgt cgcggcgcag gcgctcctga
     1561 gtccagccgg cgtaacggcg gtgcgcgctc ggcatatgct cggcgacggt ggtgtggcgg
     1621 cgcggggcat aggcgcgcag gtgggcggcg acccgcttgc cgcggtggaa cagctcgacg
     1681 gtcttggtgg tgatgcgggc cgtgatcggc tggcggatca ggccgtgcgg gaccgagtag
     1741 tagtgcttgt cgatctcgac atggtagtcg atgccgaccc ggcattcctt ccactcggcg
     1801 tattcgtaag gctcggccgg cagcggccgc agggccggtt ggtcgatctg ctggaacagc
     1861 gcgcggcggc tggcgccgag gtgacgtgac ggtttgcgat cgagctcgtc gaccagcgtg
     1921 cggatggcgt gattgaggtc ggccagggag aagaagcggc ggtggcggag cctggccagg
     1981 atccaccgct cgaccagttg caccgccacc tcgaccttgg ccttgtcgcg gggcttgcgg
     2041 ggtctggccg ggacgatcgc cgcaccgtag tgcgccgcca tgtcggcgta ggtgcggttc
     2101 aagccgggtt cgaagaagca ggccttgctg accccggcct tgagattgtc gggcacgatg
     2161 atcttgggca cgccgccgaa gaaggtgagc gcgcgcccgt gggcgccgat ccagtcgggc
     2221 agcgtctggc tccaggtggc ctcggcatag gtgaagttcg aggcgccgag cacggcgacg
     2281 aagatctgcg ccgcccggac ctcgccgctg gcgagatcgg tgacctcgac cgtctggccg
     2341 gcaaagtcga cgaacatttt ctcgccggcg cgatgggtct ggcgcatcac cggcgagagg
     2401 tggccccgcc aggcctggga gagctcgcag aagcggctca ggctgtagcc gtccggatgg
     2461 gcgccccggt actcctccca gagcagccga agcgtcacgc caggccgctt cagctcggca
     2521 tgaaccgcgg cccagttcgg ctgcggccgg tgcggtgcgc gtgcggcctc ggcggggaac
     2581 agcagtcgct cgaggtcggc gtcgctcagc cccgcgggga gtggccagcc gagcgccgcc
     2641 acgctcgccc ggcgcaggta ctcgccgacc gttgtccgcc ccaggccgag cgaacgggcg
     2701 atcacccgat cgctcatccc gcaggcgtgc ttcaaacgca gaacttcgcc cacctggcgc
     2761 atcggcaatc tcttggctgg catggcgctc cccgcatcga tcggcgtgga gagcgaccct
     2821 accggcggtt gcagatcgcc agcggcaggt ccggtccgcc cttccccaga gggtggccgg
     2881 cttcgtcgga tgcggtggcc gggttagatc ggaatcagtg tccggcttgg atcggaatca
     2941 gtgtccgctt tgcgtcggaa tacgcagtct ggcagcgctg cgatggctgc gcctagaccc
     3001 cgtcacggcc atcatgagcg aaacgtgatc cgcttgttca gataatagtt gatgggaaag
     3061 atgatgccaa gcgtgatcac ctgagccgga aggtacggga tgtaggatac caatgccccg
     3121 aatatcacgg cgttggcgcc aagggtgcac aaggtgatgc ccacgaaacg tggggccgca
     3181 acgtagtgct tggcatttga ccggaacgtg atgcgccgct ggaagtagta gttgaagccg
     3241 atcgccatcg ccaaagcgac agtggacgcc accacgggcg gcatgcgcag caattca
//
LOCUS       DATIAZ010000293         3752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_741503, whole genome shotgun sequence.
ACCESSION   DATIAZ010000293 DATIAZ010000000
VERSION     DATIAZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741503"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..802)
                     /locus_tag="VLF19_10265"
     CDS             complement(53..802)
                     /locus_tag="VLF19_10265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSE93679.1"
                     /translation="MLLRERFVDLDTPTGPMRTHLFEPTAAGRYPGVVLFSEIYQVTG
                     PIQRVARRLAGRGLIVAAPEVYHEFEPLGTVLAYDAEGTDKGNRYKIEKPVPAFDGDA
                     RAVLSHLASLPGCTGRLASMGICLGGHLSLRAAMNPGVAAAVCFYATDIHSRTLGRGK
                     KDDSLDRLGEIKGEVLMIWGRQDPHVPLEGRRTIHDGMISAGVNFTWHEFNAQHAFIR
                     DEGYRYDPALAEIGYAMVFELFDRQLTRASP"
     gene            complement(1161..3065)
                     /locus_tag="VLF19_10270"
     CDS             complement(1161..3065)
                     /locus_tag="VLF19_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HSE93680.1"
                     /translation="MRKLSGFSGLVVGVVAVLMSVYHVYARLTWYAPDQQALLFITLA
                     FSLVLSFWLWPRARGGVGDRVPWSDLALAALSLVCVGYMFVNYEYVVNRFPTAHPLSP
                     ADKLIGVLGTLLVLEATRRTIGASLPIVAVIFLAYGLAGPWLPGVLHHRGLTAEITID
                     QTWFTTEGVFGVPLGVAGTYVILFIIFGAFLEKSGAGQFFMNFANAIAGGARGGPGKV
                     SIVSSSLFGTISGSAVANVMVDGWLTIPMMKKTGFKPEAAAAIEAVASTGGQIMPPIM
                     GAAAFVMAEFLGASYTQVMIAAAIPAVFYYGALFAAVHFNAVRTGLRGIPKNELPILG
                     AIVWRQGHLFLPVIVLLVLLLQGFTPTYAAIVAAAGVVVISWVRPETGLRWRTCVAAL
                     REGAEHTVPVAMACASAGIVIGIVLQTGLALRFTSFLIQVAGGHLLLALLITMLAGII
                     LGMGMPTTPAYIMQAALLIPAVIKLGVAPMAAHMFAFYFSCLSAVTPPVALAVYAAAS
                     IGGAGLWGAGFQAMKFAAAGFIVPYFFVYNPALLFSGSWADILRALLTGTVGVIALAA
                     GLEGYFLRTATWIERGLFLMAAFLLIDPGFTTDAIGIVVLVAGLALQRQRRVATAPLA
                     ETSVIERAQP"
     gene            complement(3073..>3752)
                     /locus_tag="VLF19_10275"
     CDS             complement(3073..>3752)
                     /locus_tag="VLF19_10275"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HSE93681.1"
                     /translation="GQWKGKQTDKALYVATMYPNVWQLVVPADSPIKSLKDLKGKPVA
                     LPARGNTSLADGWENLLKANDMTLNDLGPKNYGPVASNAEAIKNRQAVAAGWFTTVPA
                     SFILDLGSTMKLRMIGLTDAEFEAVKKINPGFVRHTIKNGTYAEQGIEGDVQTFQSPT
                     ILIASSKTPADVIYRVTKAIVEGRGDYVNVTKAMKDITAQDIGRDYGMPYHPGAAKYY
                     KEIGVLK"
BASE COUNT          637 a         1233 c         1342 g          540 t
ORIGIN      
        1 ggcatctatc gcgagcaagg ccagctcgat ggccgctggg tggacaccat cgtcatggag
       61 aagctcgtgt gagctgccgg tcgaacagct cgaagaccat cgcgtagccg atctcggcca
      121 gcgccgggtc gtagcgatac ccctcgtcgc ggatgaaggc gtgctgcgcg ttgaactcgt
      181 gccacgtgaa gttcacgccg gcgctgatca tgccgtcgtg gatcgtccga cggccctcga
      241 gcgggacatg ggggtcctgg cgcccccaga tcatcagcac ctcacccttg atctcgccga
      301 ggcggtcgag ggagtcgtcc ttcttgcccc ggccgagggt gcgcgagtgg atgtcggtgg
      361 cgtagaagca cactgccgcc gccacgcccg gattcatcgc ggcgcggaga ctcaggtggc
      421 cgccgagaca gatgcccatc gacgcgagtc ggcccgtgca gcccggcagg gacgccaggt
      481 ggctcagcac ggcgcgcgcg tcaccgtcga aggcggggac aggcttctcg atcttgtagc
      541 gattgccctt gtccgtcccc tccgcgtcgt aggcgaggac ggtgccgagg ggctcgaact
      601 cgtggtagac ctccggagcc gccacgatga gtccccggcc cgctaggcgg cgcgccacgc
      661 gctggatcgg cccggtcacc tggtagatct cggagaacag caccacgccc ggataacgtc
      721 ccgctgccgt cggctcgaag agatgggtac gcatcggtcc cgtcggcgtg tcgagatcga
      781 cgaagcgctc cctgagcagc atgtcaacgg ctcccgtgga catccattgc gacgtgtcgg
      841 gcgaccgacg gcgctccccg gatcactgtg gccgttttca cggtccggat gctgccgtcg
      901 aggcaatgcc gcgagacgag ctgccggagg gcgtcgccga acacggacgc cgtagcggag
      961 atcgtcccgg cccgccggtg ccagggccga tcccgttgtt gccgccggtc acgagcgcgg
     1021 cctggcgggg agagacgagc gccttcactg gacgacgcag ggaaatcagg acgtggtgta
     1081 gaggcggagc acgccgtaga cgatggtccc cacgaggagc agggcgccga ggaggccgag
     1141 cagtgcgtcg tggagccggc tcacggctgc gcgcgctcga tgaccgacgt ctccgcgagc
     1201 ggcgccgtcg ccacccgccg ctggcgctgc agcgccagtc cggcgacgag cacgacgatc
     1261 ccgatggcgt cggtggtgaa gccggggtcg atgagcagga aggcggccat caggaagagt
     1321 ccgcgctcga tccacgtggc ggtcctcagg aagtagcctt cgaggccggc ggcgagggcg
     1381 atgaccccca ccgtgccggt gagcaaggcc cggaggatgt cggcccagga ccccgagaag
     1441 agcagcgcgg ggttgtagac gaagaagtac ggtacgatga agcccgccgc cgcgaacttc
     1501 atggcctgaa acccggcgcc ccacaacccg gcgccaccga tcgacgccgc cgcgtacacg
     1561 gcgagcgcga cgggcggggt caccgccgac aggcacgaga agtagaaggc gaacatgtgc
     1621 gcggccatcg gcgcgacgcc gagcttgatg acggcgggga tgagcagcgc cgcctgcatg
     1681 atgtacgcgg gcgtcgtcgg catgcccatg ccgagaatga tgccggcgag catggtgatg
     1741 agcagcgcga gcagcaggtg gccgcccgct acctggatga ggaacgaggt gaaccgcagg
     1801 gccagcccgg tctggagcac gatcccgatg acgatgccgg ccgaggcgca ggccatcgcc
     1861 accggcaccg tgtgctccgc gccctcgcgg agcgccgcca cgcaggtccg ccagcgcagc
     1921 ccggtctccg ggcgcaccca cgagatcacg acgacgccgg cggcagcgac gatcgcggcg
     1981 taggtgggcg tgaacccctg gaggagcagc acgagcagca cgatgacggg caggaacagg
     2041 tggccctgcc gccagacgat ggcgccgagg atcggcagct cgttcttcgg gatcccgcgc
     2101 agccccgtgc gcacggcatt gaaatggacg gcggcgaaca gcgcgccgta gtagaacacc
     2161 gccgggatgg ccgcggcgat catcacctgg gtgtaggacg cgcccaggaa ctcggccatc
     2221 acgaaggcgg ccgcgcccat gatggggggc atgatctgac cgccggtgga ggccaccgcc
     2281 tcgatcgcgg ccgccgcctc gggcttgaag ccggtcttct tcatcatcgg gatggtcagc
     2341 cagccgtcca ccatgacgtt ggcgacggcc gagcccgaga tcgtcccgaa gaggctggac
     2401 gacacgatcg agaccttgcc cggtccgccc cgcgcgccgc cggcgatggc gttggcgaag
     2461 ttcatgaaga actgcccggc gcccgacttc tcgaggaacg cgccgaagat gatgaagagg
     2521 atcacgtagg tgccggcgac gccgagcggc acgccgaaca cgccctcggt cgtgaaccag
     2581 gtctggtcga tggtgatctc ggccgtgaga ccgcggtggt gcagcacccc gggcagccac
     2641 gggccggcca ggccgtacgc gaggaagatg acggcgacga tgggcagcga ggcgccgatg
     2701 gtccgccggg tcgcctccag cacgagcagg gtgccgagca cgccgatcag cttgtcggcc
     2761 gggctcagcg ggtgggccgt ggggaagcgg ttcaccacgt actcgtagtt gacgaacatg
     2821 tagcccacgc agacgagcga cagcgcggcc agggcgaggt ccgaccacgg cacacggtcg
     2881 ccgaccccac ccctggcgcg cggccacagc cagaaggaca ggacgagact gaaggccagc
     2941 gtgatgaaga gcagcgcctg ctggtcgggc gcgtaccagg tgagacgggc gtagacgtgg
     3001 tagacggaca tgaggaccgc caccacgccg acgaccagcc cggaaaagcc gctgagcttg
     3061 cgcaccgtgc ggctacttca ggacgccgat ttccttgtag tacttcgccg cgccggggtg
     3121 atacggcatg ccgtagtcac ggccgatgtc ctgggcggtg atgtccttca tcgccttcgt
     3181 cacgttcacg tagtcgccgc gcccctcgac gatggccttc gtgaccctgt agatgacgtc
     3241 ggcgggcgtc ttggacgacg cgatgaggat ggtcggcgac tggaacgtct ggacgtcgcc
     3301 ctcgatcccc tgctccgcgt aggtgccgtt cttgatcgtg tgccgcacga agccgggatt
     3361 gatcttcttg acggcctcga actcggcgtc ggtgagcccg atcatccgga gcttcatcgt
     3421 cgagccgagg tcgaggatga acgacgcggg cacggtggtg aaccagccgg cggcgacggc
     3481 ctgccggttc ttgatggcct ccgcgttcga ggccaccggc ccgtagttct tgggtccgag
     3541 gtcgttcagc gtcatgtcgt tcgccttcag gaggttctcc cacccgtcgg ccaggctcgt
     3601 gttgccccgg gccggcagcg cgaccggctt gcccttgagg tccttcaacg acttgatggg
     3661 gctgtcggcg ggaaccacga gctgccagac gttcgggtac atggtggcga cgtacagcgc
     3721 cttgtcggtc tgcttgccct tccactgccc cg
//
LOCUS       DATIAZ010000294         5732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1794659, whole genome shotgun sequence.
ACCESSION   DATIAZ010000294 DATIAZ010000000
VERSION     DATIAZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794659"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VLF19_10280"
     CDS             <1..280
                     /locus_tag="VLF19_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HSE93682.1"
                     /translation="NFKETQLRGVRPGMRVDVAVDTFPDRRYAGVVDSISAGTGSRFS
                     LLPPENATGNWVKVVQRIPVKIVLDGQATSNPLPLRAGMSAGVTIRLR"
     gene            418..1962
                     /locus_tag="VLF19_10285"
     CDS             418..1962
                     /locus_tag="VLF19_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE93683.1"
                     /translation="MPHGTVDLVSRFLNLLLAGAALAAAAAIMVEIGRRSSGRREPLG
                     IMLCVVFLAIGVRGAVRALTDGITPDAPGSPWMALAFDGLAAVAVLAFLALRRRYAMF
                     IETADVVREYETGYAAKAREARALAQVNEELRRLDELKSEFLAMVSHELRTPLTAIIG
                     YSRLMVRQAHGPLTPKQVEYQEAIYRSAQRLSDLINDLLDVSRLEAARVELHPRPTDA
                     RQVVEHVLGVVSVAAQAKQIRLLNELDGSTPLVQADPTRLHQILVNLAGNAVKFTPAG
                     GTVRVRGARQGDQAWIAVEDTGVGIPRDELARIWDPFYQVESPMRRRHGGSGLGLSIV
                     RRLVELHGGLVRAESDGEGRGSRFSFTLPVATGPVRVERGPETEAAIEPVLAGREVLI
                     VEDDEHSRTLMRWVVEDVLGGRARVSEDGERGIAEALERPPALVLLDLMLPHVSGWEV
                     ARQLRQHAATRQLPIIAVSALARPQEREAALHAGCDAYLRKPFLPDELARVVATTLLS
                     ESVTAR"
     gene            1959..4307
                     /locus_tag="VLF19_10290"
     CDS             1959..4307
                     /locus_tag="VLF19_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93684.1"
                     /translation="MNDETPRVLLVEDDRDTTALLRDLLEAEGYVVDAVATGEAAFEA
                     LERTPDPDLLVLDLMLPGMSGYDVIERLRGEPELGRIPILVLSALSSPSARIRGLRDG
                     ADDYMTKPFLPEELIARARTLVTGRLLERRTREIQALGQITQASLTAASVEQMLQRIV
                     EILGEVFSADVAAVLLADETHAELRAGAATGTGGDLAGLTLPLREGVGAEISKSRAPL
                     VIEDGVAADPGVTSPALRRAGIRSLVVAPLVVAGTALGVVEVARRSRRVDPRAGRLLQ
                     MIADRIAVAIQRARAEGEARELADVVRRIGEGVVVADAGDGVVFANRAFGEMVGTTAE
                     SLTGCRWTDFLSPAQDIEALTAQMRLSGFHGELLLITAGGEPRPVHLTLSTVERAQGG
                     PQRIGIFRDISREHELRFRVIREQKFRTLGSLAAGVAHNINNRLTPVLGWTEMLLERL
                     GNGEAVDRDELTHALRVIHQGAADSVGTVRRLQEYSRPARVKGPEAVALREVVEQLLA
                     LTRPQWDNEAARRGVRYEIALRIEPAPPILAVASEIREALLNILENAFAAMPDGGRLT
                     IEIRGQDDRAVVAVSDTGRGMSAEVQRVAFEPFFTTRASEGGSGLGLSLAQEIVQRYG
                     GSIAVQSREGAGTTFTLSFPSVSADGTRVAAFLPVLEPLRVLVVEDEPEVLDLVRAVL
                     AHAGHAVLAAASGREAVTLFGREPVDLVITDLGMPGMTGTALAAELKRLRRVPVVLLT
                     GWADELDRDSTPDVDVILAKPFSREQFFAALARAVPGRVRSS"
     gene            complement(4419..5195)
                     /locus_tag="VLF19_10295"
     CDS             complement(4419..5195)
                     /locus_tag="VLF19_10295"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSE93685.1"
                     /translation="MSYLQAVVLGVVQGLTEFLPISSSGHLVLVPRLIGWPDQGLAFD
                     AAIHLGTLAALVAYFRAELRAMLIGGLSRRLVLVLLVASLPALVIGALFNHWIEAHLR
                     SPVVIAASTAFWAVVMWIADRSVRPPPSGEPLERVGWDQGLAIGCAQALALVPGTSRS
                     GITMTTGLLGGLDRATAARFSFLLGIPVTAAAGGYKMLKILGTGLAAADAGPLAVAVV
                     AAFVSGWAAVWCLVGYLRRRSLTPFVVYRLLLAAVIVALI"
     gene            5259..>5732
                     /locus_tag="VLF19_10300"
     CDS             5259..>5732
                     /locus_tag="VLF19_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HSE93686.1"
                     /translation="MPKTAGIILIGNEILSGKIEDANATYLCRELRGLGVDVRRIAVV
                     PDEVAVIAAEIATVSRDHDVVFTSGGVGPTHDDVTIEGAARAMGVAVTRHPDLVALLR
                     GYYGDRLNEAQLKMAEVPEGAELIGGEVLRFPTILIRNVYVLPGVPEIFRQKFEAI"
BASE COUNT          834 a         2013 c         2109 g          776 t
ORIGIN      
        1 caacttcaag gagacccagc tccgcggcgt gcggccgggg atgcgggtgg acgtggccgt
       61 cgacaccttc ccggaccgcc ggtacgcggg cgtcgtcgat tcgatcagcg ctggcacggg
      121 ctcgcggttc tcgctgctgc cacccgagaa cgcgaccggg aactgggtga aggtggtcca
      181 gcgtatcccc gtgaagatcg tgctcgacgg ccaggcgacc tcgaatccgc tcccgctgcg
      241 cgccgggatg tcggcgggcg tcaccatccg gctacgctga gccaacgtgc cgcagcgcgg
      301 cgcgtgtaag aactactgcg tgggcgagta attcgttctc gcgagctgag aaagtgcttt
      361 cacggggtcc tcgtgtcatg ggacggtgat aggatccggc ctcgggaacc cttctgcatg
      421 ccacacggga ccgtcgacct cgtctcccgt ttcctgaacc tgctgctcgc cggtgcggcg
      481 ctggcggccg cggcggcgat catggtcgag atcggacggc ggtcgtcggg ccggcgggaa
      541 ccgctcggca tcatgctctg cgtcgtcttc ctcgccatcg gcgtgcgcgg cgccgtgcgg
      601 gcgctcactg acggaatcac gcccgacgcg cccggctcgc cgtggatggc cctggccttc
      661 gacgggctcg ccgcggtcgc ggtcctcgca ttcctcgcgc tgcggcggcg ctacgcgatg
      721 ttcatcgaga cggccgatgt cgtccgagag tacgagacgg gctacgcggc caaggcgcgc
      781 gaggcccgcg cgctggcgca ggtgaacgag gagctgcgcc ggctcgacga gctgaagtcc
      841 gagttcctgg cgatggtcag ccacgagctg cgcacgccgc tcacggccat catcggctac
      901 agccggctca tggtccgaca ggcccacggc ccgctcacac cgaagcaggt ggagtaccag
      961 gaggcgatct accggagcgc ccagcgtctg agcgatctga tcaacgacct gctcgacgtg
     1021 tcgcgtctcg aggcggcgcg ggtcgagctg cacccccggc cgaccgacgc gcggcaggtg
     1081 gtcgagcacg tgctgggtgt ggtgagcgtg gccgcgcagg ccaagcagat ccggctgctg
     1141 aacgagctgg acggctcgac gccgctcgtc caggcggatc ccacgcggct ccaccagatc
     1201 ctcgtcaacc tcgccggcaa cgccgtcaag ttcacgcccg ccggcggcac ggtgcgcgtg
     1261 cgcggcgcga ggcagggcga ccaggcgtgg atcgccgtcg aggataccgg cgtcggcatc
     1321 ccgcgggacg agctggcgcg gatctgggac ccgttctacc aggtcgagtc gcccatgcgg
     1381 cgccggcacg gcgggtcggg cctcgggctg tcgatcgtgc gccgcctcgt ggagctgcac
     1441 ggcggactcg tgcgggcgga gagcgacggt gaaggtcggg gcagccgctt cagcttcacg
     1501 ctgccggtcg ccaccggccc cgtgcgcgtg gaaagggggc cggagacgga ggcggcgatc
     1561 gagcccgtgc tggccggccg cgaggtgctc atcgtcgagg acgacgagca cagccgcacg
     1621 ctcatgcgct gggtcgtgga agacgtgctg ggcggccggg cgcgcgtctc ggaggacggt
     1681 gagcgcggga tcgccgaggc gctcgagcgg ccgccggccc tggtgctcct cgacctcatg
     1741 ctcccgcacg tgtccggctg ggaggtggca aggcagctcc gccagcacgc ggccacccgg
     1801 cagctgccga tcatcgcggt gtccgcgctg gcgcggcccc aggaacgcga ggcggccctc
     1861 cacgccgggt gcgacgcgta cctgcgcaag ccgttcctcc ccgacgagct ggcgcgggtc
     1921 gtggcgacga cgctgctgag cgagagtgtg acggcccggt gaacgacgag acgccccgcg
     1981 tcctgctcgt cgaggacgac cgcgacacca cggcgctgct ccgcgacctg ctcgaggccg
     2041 aaggctacgt cgtggacgcc gtcgccacgg gtgaggcggc cttcgaggcc ctcgagcgca
     2101 cgcccgatcc cgacctgctc gtcctcgacc tcatgctccc cggcatgagc ggctacgacg
     2161 tcatcgagcg gctgcggggc gagcccgagc tcggccgcat cccgatcctc gtcctcagcg
     2221 ccctcagctc gccctccgcc cgcatccgcg gtctccgcga cggggcggac gactacatga
     2281 ccaagccgtt ccttcccgag gagctgatcg cccgcgcacg cacgctggtc acggggcggc
     2341 tcctcgagcg gcgcacgcgg gaaatccagg cgctgggaca gatcacgcag gccagcctca
     2401 cggccgcgag cgtggagcag atgctgcaac ggatcgtgga gatcctgggc gaggtcttct
     2461 ccgccgacgt ggcggccgtg ctcctggccg acgagaccca cgccgagctc cgggcaggcg
     2521 cggcgaccgg caccgggggt gacctcgccg gcctcacgct gccgctcagg gagggcgtgg
     2581 gcgcggagat ctcgaaatcg cgcgcgccgc tcgtgatcga ggacggcgtg gcggccgatc
     2641 ccggggtcac cagccccgcg ctgcggcgtg ccggcatccg ttcgctcgtc gtcgcgccgc
     2701 tcgtcgtcgc gggaaccgcg ctcggcgtcg tggaggtcgc gcgccggagc cggcgcgtcg
     2761 atccgcgcgc cgggcgcctg ctgcagatga tcgccgaccg gatcgccgtc gccatccagc
     2821 gcgcccgcgc cgagggcgaa gctcgcgagc tagccgacgt ggtgaggcgg atcggcgagg
     2881 gcgtcgtcgt cgccgacgcc ggcgacggcg tcgtcttcgc caaccgcgcg ttcggagaga
     2941 tggtgggcac gaccgccgag agcctcaccg ggtgccggtg gacggacttc ctctcgccgg
     3001 cccaggacat cgaggcgctc accgcccaga tgcgcctgtc gggcttccac ggggagctct
     3061 tgctcatcac cgcgggcggc gagccgcgtc cggtccacct cacgctgtcc accgtggagc
     3121 gcgcgcaggg cggaccgcag cggatcggga tcttccgcga catcagccgc gagcacgagc
     3181 tgcgcttccg cgtgatccgc gagcagaagt tccgcacgct cggctcgctg gccgccggcg
     3241 tcgcccacaa catcaacaac cgcctcacgc ccgtgctcgg ttggaccgag atgctcctcg
     3301 agcggctcgg aaacggcgag gcggtggacc gcgacgagct gacgcacgcg ttgcgcgtga
     3361 tccaccaggg cgcggccgac agcgtgggca ccgtgcgacg gctccaggag tactcgcggc
     3421 cggcgcgcgt gaaggggccc gaggcggtag cgctccgcga ggtcgtcgag cagctgctcg
     3481 cgctgacccg tccccagtgg gacaacgagg cggcgcggcg cggcgtccga tacgaaatcg
     3541 ccctgcgcat cgagcccgcg ccgcccatcc tggccgtggc cagcgagatc cgggaggcat
     3601 tgctgaacat cctcgagaac gcgttcgcgg cgatgcccga cggcggtcgc ctgactatcg
     3661 agatccgggg gcaggacgac cgcgccgtgg tggcggtgtc ggacacggga cggggcatga
     3721 gcgccgaggt tcagcgtgtg gccttcgagc cgttcttcac cacccgcgcg agcgagggcg
     3781 gcagcgggct cggtctctcg ctggcgcagg agatcgtcca gcgctacggc ggcagcatcg
     3841 cggtccagag ccgtgagggc gcggggacga cgttcacgct ctcgtttccc tcggtcagcg
     3901 ccgacggtac ccgcgtggcg gcattcctgc cggtgctcga gccgctgcgc gtcctcgtcg
     3961 tggaggacga gcccgaggtg ctcgacctcg tgcgcgccgt gctggcccac gccggccacg
     4021 ccgtgctcgc ggcggcctcc ggccgcgagg cggtcacgct gttcggccgc gagccggtgg
     4081 acctcgtgat caccgatctg ggcatgcccg gcatgaccgg caccgcgctg gccgccgagc
     4141 tcaagcgcct gcgccgggtc ccggtggtgc tgctgacggg ctgggccgac gagctcgacc
     4201 gagactcgac gccggacgtc gacgtgatcc tcgccaagcc gttctcgcgc gagcagttct
     4261 tcgcggcgct ggcgcgcgcc gtccccgggc gcgtgcggtc ctcgtagtcg cccgcgctcc
     4321 gccgggttca cggtccacgg cgatcctctc gccgaatcgc gtcgtgacgg ccgccgatgc
     4381 gattggacca cggttccgtg gcggcccacc cactgagcct agatcagggc gacgatgaca
     4441 gcggccagga gcaggcggta gacgacgaac ggtgtcaggg accgccgcct gaggtagccg
     4501 acgaggcacc agacggccgc ccagcccgac acgaaggcgg cgacaacggc gactgcgagc
     4561 gggccggcat cagccgcagc gagaccggtg ccgaggatct tgagcatctt gtatccgccg
     4621 gccgccgcgg tgaccgggat gccgaggaga aacgagaacc gggccgcggt cgcgcggtcg
     4681 aggccgccga gcagcccggt ggtcatggtg atacccgagc gcgaggtgcc gggcacgagg
     4741 gcgagggcct gcgcgcatcc gatggcgagc ccctggtccc agcccacccg ctcgagcggc
     4801 tcaccggacg gtggaggccg cacgctgcgg tcggcgatcc acatcaccac cgcccagaag
     4861 gccgtcgacg cggcgatgac gaccggcgat ctcagatgtg cctcgatcca gtggttgaag
     4921 agcgcgccga tgacgagcgc aggaagcgag gcgacgagca gcaccagcac gaggcgccgt
     4981 gacaggccgc cgatgagcat cgcccggagc tccgcccgga agtaggcgac gagagcggcg
     5041 agcgtgccga ggtggatggc cgcgtcgaag gcgagaccct ggtcgggcca gccgatcagg
     5101 cgcggcacga ggacgagatg ccccgatgac gagatgggaa ggaactcggt gagtccctgc
     5161 acgacgccga gcacgaccgc ctggaggtag ctcacgcgac gtctggattg tagcaccgtc
     5221 gacagtcgcc ggccgcgaag cgtacaatcc cggaggccat gccgaagacg gcggggatca
     5281 tcctcatcgg caacgagatc ctgtcgggca agatcgagga cgccaacgcc acctacctct
     5341 gccgcgagct gcgcgggctc ggcgtggacg tccgtcgaat cgcggtcgtc ccggacgagg
     5401 tcgccgtcat cgcggcggag atcgccaccg tcagccgcga ccacgacgtg gtcttcactt
     5461 cgggcggcgt gggaccgacg cacgacgacg tcaccatcga gggcgcggcg cgggccatgg
     5521 gcgtggcggt cacacgccat ccggatctgg tcgcgctgct gcgcggctac tacggcgatc
     5581 ggctcaacga ggcccagctc aagatggccg aggttcccga gggggcggag ctgatcggcg
     5641 gcgaggtgct tcgcttcccg accatcctga tccggaacgt ctacgttctg cccggcgtgc
     5701 cggagatctt ccggcagaag ttcgaggcga tc
//
LOCUS       DATIAZ010000295         3272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_214821, whole genome shotgun sequence.
ACCESSION   DATIAZ010000295 DATIAZ010000000
VERSION     DATIAZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214821"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VLF19_10305"
     CDS             <1..296
                     /locus_tag="VLF19_10305"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSE93687.1"
                     /translation="VSEARGARLTLDSSTHPKPDALTNSEAFLAGVSSCGVTLIEAHA
                     QQTGVPLTRVEVTIEGVRAAADPSRFSRVAMRFALAGVSQAQAEDLVRTYQGR"
     gene            complement(414..1820)
                     /locus_tag="VLF19_10310"
     CDS             complement(414..1820)
                     /locus_tag="VLF19_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93688.1"
                     /translation="MAGNVVRLVEAVVARAPGRPALTWQGGTITYGRLDRLAGGFARF
                     LAHQGVRAGDRVALAIGNRWTFAVAVLGALKAGMTVAPLDPLLKAEERAAIVADLSPR
                     IVAHEVLGDEVAWPATDGDAPALVLYTSGSTGAPKGAVLSQGALAAALASWAGPVMAL
                     TPDDVVLATLPLAHSFGFNGALLAPLTAGAHVVLVERFTPETALAAIHEHGVTVFPGV
                     ATMFRRVLDSPAFARETLASLRLAVSGAAPCPWSLAAEWRERTGTRILRGYGMTELFR
                     PLSYLAADPTELPDAVGRPVPGVEVCVVDDAGRPLPPGAEGELLIRSPSAMDAYLGAP
                     EETRAVLSDGWFHTGDLARIGPEGYVSIEGRKRERILRGGYSIFPREVETVLAGHPAV
                     AEAAVVAEPHPELGEEVAAFVALREGARVSPEDLVAWCRERLAAFKYPRRVVVLDRLP
                     KSANGKILKTRLIADRAQ"
     gene            complement(1820..3175)
                     /locus_tag="VLF19_10315"
     CDS             complement(1820..3175)
                     /locus_tag="VLF19_10315"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSE93689.1"
                     /translation="MPQDLRGYLDLVKRRRPDELVVVSREVDPAFEITALVVKLEREA
                     RRRPVLIFENVKGTRFPVLTNLHASRGRLALALNAPPEELLRTYLGAMERAIPPRVVP
                     AGPVKDVVLRGGDVDLRALPQIVHHERDGGVFLTAAISFAKDPTAEIWNCAYNRLMLK
                     GPDTTSIHLTLGKHLWEFHRIAEKRDEPLPVAFAIGVHPAIALGALAIGSVDEDERAI
                     IGALLGEPLELVKCETSDVLVPAHAEIVIEAEILPHERTAEGPFGEFTGYSLGERQRE
                     VVRIKAITHRRGAIFQDISVAHLDHLLLSTIPIEANLYRAVRAMVPSVTAVRVPAPFT
                     CYVSLEQRIPGQAKNAILAVLGADLYMKRVVVVDHDVDVFDDRQINWAIATRSQPDRD
                     ILTVTHARGSDLDPSTKEDGYTAKWGVDATAKPSLAAYTPRHRVPPEVYRRVELKDFL
                     P"
BASE COUNT          460 a         1151 c         1239 g          422 t
ORIGIN      
        1 cggtgagcga ggcgcggggc gcccggctca cgctggactc gtccacccac cccaagcccg
       61 acgccctcac gaacagcgag gccttcctgg ccggcgtgtc gtcgtgcggc gtgacgttga
      121 tcgaggcaca cgcgcagcag accggcgtgc cgctcacgcg cgtggaggtg acgatcgagg
      181 gcgtgcgggc ggccgcggat ccgtcgcggt tctcgcgggt cgccatgcgc ttcgcgctgg
      241 ccggcgtcag ccaggcccag gcggaagacc tcgtcaggac ctaccaaggg cgctgacccc
      301 tctaccgcac ggtcgcgggc gcgaccgcgg tgaaggtcga agtcagcacg ctcccggctc
      361 gataggcgcg cgcgtacggc gaagaacccg ggaggtcggg cgaggggcgc gcgtcactgc
      421 gcgcggtcgg cgatgagacg ggtcttcaag atcttgccgt tcgcgctctt tggcaatcgg
      481 tccagcacga cgacgcggcg cggatacttg aaggcggcca gccgctcgcg gcaccaggcg
      541 acgaggtcct cggggctcac gcgggcaccc tcgcgcagcg cgacgaacgc ggccacttcc
      601 tcgccgagct ccgggtgggg ttccgcgacg accgccgcct cggcgacggc cgggtggccg
      661 gccagcaccg tctcgacctc gcgcggaaag atcgagtagc caccgcgcag gatgcgctcg
      721 cgcttgcgtc cctcgatgct cacgtagccc tccggcccga tccgcgcgag gtcgccggtg
      781 tggaaccagc cgtcgctcag gaccgcgcgc gtctcctcgg gcgcgccgag gtaggcgtcc
      841 atcgcgctcg gcgagcggat cagaagctcg ccctccgccc ccggcggcag cggccggccg
      901 gcgtcgtcca cgacgcagac ctcgacaccg ggcacggggc ggccgacggc gtcgggcaac
      961 tcggtcggat cggcggccag gtaggagagc gggcggaaca gctcggtcat cccgtagccg
     1021 cgcaggatcc gcgtgcccgt ccgctcgcgc cactccgcgg cgagcgacca cgggcacggc
     1081 gccgcccccg acacggcgag gcgcaggctg gcgagcgtct cgcgcgcgaa ggccggcgag
     1141 tcgagcacgc gccggaacat ggtcgccacg ccggggaaga cggtgacgcc gtgctcgtgg
     1201 atggccgcca gcgccgtctc gggggtgaag cgctcgacga gcaccacgtg ggcgccggcc
     1261 gtcagcggcg ccagcagcgc gccgttgaag ccgaacgagt gcgcgagcgg cagcgtggcc
     1321 agcaccacgt cgtcgggcgt gagcgccatc accgggcccg cccacgacgc cagcgcggcg
     1381 gcgagggccc cctgcgagag cacggcgccc ttcggggcgc cggtggagcc cgacgtgtag
     1441 aggacgagcg ccggcgcgtc gccgtcggtg gccggccacg cgacctcgtc accgaggacc
     1501 tcgtgggcga cgatgcgcgg cgagagatcg gcgacgatcg cggcccgctc ctcggccttg
     1561 agcagcgggt ccagcggggc gaccgtcatg ccggccttga gcgcgccgag caccgccacc
     1621 gcgaacgtcc agcggttgcc gatggccagc gcgacgcggt cgcccgctcg cacgccctgg
     1681 tgtgcgagga agcgggcgaa gccgccagcc aggcggtcga gccggccgta ggtgatcgtc
     1741 ccgccttgcc aggtgagcgc gggccggccc ggcgcgcggg ccacgacggc ctcgacgagc
     1801 cggacgacgt tgccggccac tagggcagga agtccttcag ctcgacccgg cggtagacct
     1861 cgggcggcac gcgatggcgc ggtgtgtagg cggcgagcga gggcttcgcc gtcgcatcca
     1921 cgccccactt cgcggtgtag ccgtcctcct tcgtggaggg atcgaggtcg gacccgcgcg
     1981 catgcgtgac ggtgaggatg tcgcggtcgg gctggctgcg cgtggcgatg gcccagttga
     2041 tctggcggtc gtcgaagacg tccacgtcgt ggtccaccac gaccacgcgc ttcatgtaca
     2101 ggtcggcgcc gagcacggcg aggatcgcgt tcttcgcctg tccgggaatg cgctgctcca
     2161 gcgagacata gcaggtgaac ggcgccggca cgcgcaccgc ggtcaccgac ggcaccatgg
     2221 cgcggacagc gcggtagagg ttcgcctcga tggggatggt ggacagcagc aggtggtcga
     2281 ggtgggccac cgagatgtcc tggaagatcg cgccgcgacg gtgcgtgatg gccttgatcc
     2341 ggacgacctc gcgctggcgc tcgccgaggc tgtagccggt gaactcgccg aacggtccct
     2401 cggcggtgcg ctcgtgcggc aggatctccg cctcgatcac gatctccgcg tgggccggca
     2461 cgagcacgtc ggaggtctcg cacttgacga gctccagcgg ctcgccgagc agcgcgccga
     2521 tgatcgcgcg ctcgtcctcg tcgaccgagc cgatggccag cgccccgagc gcgatcgccg
     2581 ggtggacacc gatggcgaac gccaccggca gcggctcgtc gcgcttctcg gcgatgcggt
     2641 ggaactccca gaggtgcttg ccgagcgtga ggtggatcga cgtcgtgtcg ggccccttga
     2701 gcatgagacg gttgtaggcg cagttccaga tctccgccgt cgggtccttc gcgaacgaga
     2761 tcgccgcggt gaggaacacg ccgccgtccc gctcgtggtg cacgatctgc ggcagtgcgc
     2821 gcaggtcgac gtctccgccg cgcagcacga cgtccttgac gggcccggcg ggcaccacgc
     2881 gtggcgggat cgcgcgctcc atggccccga ggtaggtgcg caagagctcc tcgggcggcg
     2941 cgttcagcgc cagggccagg cgcccgcggc tcgcgtgcag attggtcagc accggaaagc
     3001 gcgtgccctt gacgttctcg aagatcagca ccgggcggcg ccgggcttcc cgctcgagct
     3061 tgaccacgag ggcggtgatc tcgaaggcgg ggtcgacctc cctggacacg acgaccagct
     3121 cgtcgggccg cctgcgcttg acgagatcga ggtagccgcg gaggtcctgg ggcacggccg
     3181 cgattatact aggctcccca cggtgccgga cgccgaggga gcgacttgtt gccggcgacg
     3241 cgggcagggc tgcgggcgcc accccggggg cg
//
LOCUS       DATIAZ010000296         5503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_479888, whole genome shotgun sequence.
ACCESSION   DATIAZ010000296 DATIAZ010000000
VERSION     DATIAZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479888"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VLF19_10320"
     CDS             complement(<1..162)
                     /locus_tag="VLF19_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSE93690.1"
                     /translation="MPRLTIDGREVEVSPGTNLIEAARQAGVEVPHYCYHPGLSIAGQ
                     CRLCMVDIDK"
     gene            complement(238..1461)
                     /gene="nuoF"
                     /locus_tag="VLF19_10325"
     CDS             complement(238..1461)
                     /gene="nuoF"
                     /locus_tag="VLF19_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSE93691.1"
                     /translation="MTGEKLLTRNFDRPDSHTLAAYEETGGYRAWRTAQEMEPAAITE
                     AVKKSNLRGLGGAGFPTGAKWSFVPQGHTGPVYLVINADEGEPGTFKDRYLLERDPHA
                     LIEGMLISARAIRCEKAFVYVRGEYVEPWRRLSTAVTEAYAAGHLGRGLEVVVHRGAG
                     AYICGEETGLLSSLEGKKGWPKIKPPFPAVKGAFGQPTIVNNVETIAHVPHIISRGPE
                     WFASLGTRTQGGTRMYSVSGRVRKPGVYETSVGITLRQLIELAGGVSGTGRLKGVVPG
                     GGSAAIVTADEIDVTMDVDGLKSVGSMIGSAGVIVMDESVSIPEALLVLARFYAHESC
                     GQCTPCRESTGWIYKMVHRIVAGRGRKEDLDTILDVARRGAGTTICAFYDGAVGPYVS
                     YIEKFRHEFEALIPK"
     gene            complement(1458..1937)
                     /locus_tag="VLF19_10330"
     CDS             complement(1458..1937)
                     /locus_tag="VLF19_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSE93692.1"
                     /translation="MKTPVQFSPEQLAEVRRLQGLYPERRGALLPVLHMAQAAFGYIS
                     LEVEDYVAGLFELSPAHVHEVVTFYTLYFREPAGRHVVSVCHNLSCHLAGAPGIIEHL
                     KTRLGIDVGETTGDGRVTLLSVECLCACEHAPMMQVDDRYEFDLTPDKIDRTLERLT"
     gene            complement(2035..3414)
                     /locus_tag="VLF19_10335"
     CDS             complement(2035..3414)
                     /locus_tag="VLF19_10335"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSE93693.1"
                     /translation="MARPTTTREFMLGEGAGTGSGGTQAMHLGVGPQHPAMHGIIRIV
                     TELDGETIVNADVEIGYLHRAFEKDCEVGPYNNAIPYTDRLNYVSPLINNFGYCSAVE
                     KLLGIDVTERCQHIRVAMSEISRICDHLTCIGASAMELGAFTVFLYMIKAREFLWELV
                     EAVTGARLTISYGRVGGVKADLPPGFAEQARSAFKQTREVLEEVHRLLTGNRIFMDRM
                     VDVGALSREETIAWAITGPMLRAVGVPYDVRRAQPYYVYDRLEWEVPTQTAGDNYARY
                     LVRMAEMEQSMRIAEQALADLPGGPIQVDFEGRPVDPAAYVDQGKQGKTEGLLLLPIR
                     LSPNLQGQERPAHDRVNASDKSVVLPPKETTYGSIEGLMNHFMLIMEGYGIRPPPGEA
                     YFAVEGANGELGFYVVSDGGDRPYRVRCRPPCLPPLAALHRMIEGEMLADLIPTFGSV
                     NMIGGELDR"
     gene            complement(3414..3911)
                     /locus_tag="VLF19_10340"
     CDS             complement(3414..3911)
                     /locus_tag="VLF19_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSE93694.1"
                     /translation="MDGAAILATLAERFGAAIVETHAHRGDHTAVVTREALVDVLRFG
                     RDDGRLAFDLLMDLTAVDYSKFPGREDGPRFEVVYHLYSVRHNHRVRLKTRVAEDDAV
                     VPTAAGLWPIADWLEREVWDMFGIRFEGHPNLRRLLLYEEFVGHPLRKDYPINRRQPL
                     VGPRI"
     gene            complement(3911..4369)
                     /locus_tag="VLF19_10345"
     CDS             complement(3911..4369)
                     /locus_tag="VLF19_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HSE93695.1"
                     /translation="MGVGGFFTSKLDEAIGWARKYSIFQYPFVTACCGMEYMATSCSH
                     YDIDRFGAGLPRFSPRQADVLFVVGTISHKMAPVLKRIYDQMTEPKWVVAFGVCTCTG
                     GFYDNYATVQGIDTIIPVDVYIPGCPPRPESVIDGLMKLQEKIAVGAQRF"
     gene            complement(4360..4716)
                     /gene="ndhC"
                     /locus_tag="VLF19_10350"
     CDS             complement(4360..4716)
                     /gene="ndhC"
                     /locus_tag="VLF19_10350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSE93696.1"
                     /translation="MTTEYATILMVFGFAAVVAGALLGIPLLIAPRRLTPVKVEPFEC
                     GKDPLEISEGRFAIKFSTIAIFFILIDIELLFIWPWAMLYRGLGWFGFAEMMVFMGVL
                     MLGFLYIWRKRGFEWE"
     gene            complement(4713..>5503)
                     /gene="lipA"
                     /locus_tag="VLF19_10355"
     CDS             complement(4713..>5503)
                     /gene="lipA"
                     /locus_tag="VLF19_10355"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSE93697.1"
                     /translation="REWNLHSVCEEARCPNIGECWEDATATFMILGDICTRNCGYCAI
                     AHGRPAWEDREEPERIGRAVAALGLDYVVVTSVNRDDLADGGAAHWAATIRAVRRHAP
                     ACRVEALIPDFQGQAEALAAVIDARPDVLNHNTETVPRLYKVARHGGRYDRVLELFRR
                     ARAAAPDLVTKSGLILGLGETPEELLAVMRDLRAADVRILTLGQYLRPSAQHLPVERY
                     YPPDEFAALAAIGRGLGFAHVEAGPLVRSSYHARRQTQAAEHPA"
BASE COUNT          833 a         1920 c         1906 g          844 t
ORIGIN      
        1 cttgtcgatg tccaccatgc agagccggca ctggccggcg atcgacagcc cggggtgata
       61 gcagtagtgc ggcacctcga cccccgcctg gcgcgcggcc tcgatgaggt tcgtgcccgg
      121 cgacacttcg acttccctgc cgtcgatcgt gagcctgggc atttcagtcg gatggggccc
      181 cgatggcccc ctccggaatc gtcccgggga cacgggtcgc gccggccatt gcggtggtca
      241 tttcgggatc agggcttcga actcgtggcg gaacttctcg atgtaggaga catagggacc
      301 cacggcaccg tcgtagaagg cgcagatggt cgtccccgcg ccgcgcctgg cgacgtccag
      361 gatcgtgtcg aggtcctcct tgcgcccgcg gccggcgacg atccggtgga ccatcttgta
      421 gatccagccg gtggattcgc ggcagggcgt gcactgtccg cacgactcgt gggcgtagaa
      481 ccgcgccagc accaggagcg cctccgggat cgacacgctc tcgtccatga cgatcacgcc
      541 ggccgagccg atcatcgagc cgacgctctt cagaccgtcc acgtccatcg tcacgtcgat
      601 ctcgtccgcg gtgacgatcg cggccgagcc gccgccgggc accacgccct tgagccggcc
      661 cgtgccgctg accccgccgg ccagctcgat gagctgccgc agcgtgatgc ccaccgaggt
      721 ctcgtagacg ccgggcttgc gcacgcgtcc ggacacggag tacatgcggg tcccgccctg
      781 cgtcctggtg ccgagggagg cgaaccactc ggggccgcga ctgatgatgt gcggcacgtg
      841 ggcgatggtc tccacgttgt tgacgatcgt gggctgcccg aaggcgccct tgacggcggg
      901 gaacgggggc ttgatcttcg gccagccctt cttgccttcc agcgaggaca gcagcccggt
      961 ctcctcgccg cagatgtagg cgccggcgcc gcggtggacg acgacctcca gaccgcgacc
     1021 caggtggccg gcggcgtagg cctcggtcac cgccgtgctg aggcgccgcc acggctcgac
     1081 gtactcgccc cggacgtaga cgaacgcctt ctcgcagcgg atggcccggg ccgagatgag
     1141 catgccctcg atcagcgcgt gtgggtctcg ctccaggagg tagcggtcct tgaacgtgcc
     1201 tggctcgccc tcgtcggcgt tgatgacgag gtagacgggg cccgtgtggc cctggggaac
     1261 gaacgaccac ttggcccccg tcggaaaccc ggcgccgccg agcccgcgca ggttggactt
     1321 cttcaccgcc tcggtgatcg ccgccggctc catctcctgc gccgtccgcc acgcccggta
     1381 accgcccgtc tcctcgtagg ctgccagcgt gtgggagtcg ggtcggtcga agttccgggt
     1441 caggagcttc tcgccggtca cgtgagcctc tcgagggtcc ggtcgatctt gtccggcgtg
     1501 aggtcgaact cgtagcggtc gtccacctgc atcatcggcg cgtgctcgca ggcgcagagg
     1561 cactcgaccg agagcagcgt cacccggccg tcgccggtcg tctcgcccac gtcgatcccg
     1621 agccgcgtct tgaggtgctc gatgatcccc ggcgctccgg cgaggtggca ggagaggttg
     1681 tgacagacgg agaccacgtg gcggcccgcc ggctcgcgga agtagagggt gtagaacgtc
     1741 accacctcgt gcacgtgcgc cggagagagc tcgaagaggc cggccacgta gtcctcgacc
     1801 tcgagcgaga tgtagccgaa ggccgcctgc gccatgtgca gcacgggcag gagcgcgccc
     1861 cgccgctcgg gatagagccc ctgcaggcgg cggacctcgg cgagctgctc gggagagaac
     1921 tgcaccggcg tcttcatggg gcgcccgtcc atcgctctcg agctcgacac cgagaacatc
     1981 gaccagcgaa ggccggcccg gccggccttg ccgtgcggag ctcgcctggc gagctcaccg
     2041 gtcgagctcc cccccgatca tgttcaccga gccgaacgtc gggatcaggt cggcgagcat
     2101 ctccccctcg atcatgcggt gcagtgcggc cagcggcggc aggcacggcg gccggcagcg
     2161 cacgcgatag ggccggtcgc ccccgtcgga gacgacgtag aagccgagct cgccgttggc
     2221 gccctccacg gcgaagtacg cctcgcccgg cggggggcgg atgccgtagc cctccatgat
     2281 cagcatgaag tggttcatca ggccctcgat ggacccgtac gtggtctcct tcggcggcag
     2341 caccacgctc ttgtccgacg cgttgacgcg gtcgtgggcc gggcgctcct ggccctggag
     2401 gttcggcgac agcctgatgg gcaggagcag caggccctcg gtcttgccct gcttgccctg
     2461 gtcgacgtac gccgccgggt cgacgggccg gccctcgaag tccacctgga tgggcccgcc
     2521 gggaaggtcg gcgagcgcct gctcggcgat gcgcatcgac tgctccatct cggccatccg
     2581 cacgaggtaa cgcgcgtagt tgtcgccggc cgtctgcgtg ggcacctccc actcgaggcg
     2641 gtcgtagacg tagtagggct gcgcgcggcg cacgtcgtac ggcacgccga cggcgcgcag
     2701 catcgggccc gtgatcgccc acgcgatcgt ttcctcgcgc gacagcgccc ccacgtccac
     2761 catccggtcc atgaagatgc ggttacccgt cagcagccgg tggacctcct ccagcacctc
     2821 gcgcgtctgc ttgaacgccg agcgcgcttg ctccgcgaag ccgggcggca ggtcggcctt
     2881 cacgccgccg acgcgcccgt aggagatcgt cagccgcgcc ccggtcaccg cctccaccag
     2941 ctcccacagg aactcgcggg ccttgatcat gtagaggaag accgtgaagg cccccagctc
     3001 catcgcggag gcgccgatgc acgtgaggtg gtcgcagatc cgcgagatct cggacatggc
     3061 gacccggatg tgctgacagc gctcggtgac gtcgatgccg agcagcttct cgaccgccga
     3121 gcagtacccg aaattgttga tgagcggcga cacgtagttc aggcggtcgg tgtacgggat
     3181 cgcgttgttg taagggccga cctcgcagtc cttctcgaac gcccggtgca ggtaaccgat
     3241 ctcgacgtcg gcgttcacga tggtctcgcc gtccagctcg gtgacgatgc ggatgatgcc
     3301 gtgcatggcc gggtgctggg gcccgacgcc gaggtgcatg gcctgcgtgc cgccgctgcc
     3361 ggtgcccgcc ccctcgccca gcatgaactc ccgggtggtc gtgggccggg ccatcagatc
     3421 cgcgggccca cgagaggctg ccgccggttg atcgggtagt ccttgcggag cgggtgaccc
     3481 acgaactcct cgtagagcag caagcgccgg aggttcgggt ggccctcgaa gcggatgccg
     3541 aacatgtccc acacctcgcg ctcgagccag tcggcgatgg gccacaggcc ggccgcggtc
     3601 ggcaccaccg cgtcgtcctc ggccacgcgc gtcttgaggc gtacgcggtg gttgtgacgg
     3661 acggagtaga ggtggtagac gacctcgaag cgcgggccgt cctcgcggcc ggggaacttc
     3721 gagtagtcca ccgccgtgag gtccatcagc aggtcgaagg ccagccggcc gtcgtcgcgg
     3781 ccgaaccgta gcacgtcgac gagagcctcg cgcgtgacga ccgccgtgtg gtcgccgcga
     3841 tgcgcgtggg tctcgacgat cgccgcgccg aagcgctcgg cgagggtggc caggatcgcg
     3901 gcaccgtcca ctagaacctc tgggccccca ccgcgatctt ctcctggagc ttcatgagcc
     3961 cgtcgatcac gctctccggc cggggcgggc agcccgggat gtagacgtcc acggggatga
     4021 tcgtgtcgat gccctgcacg gtggcatagt tgtcgtagaa gcccccggtg caggtgcaga
     4081 caccgaacgc caccacccac ttcggctctg tcatctggtc gtagatgcgc ttgagcaccg
     4141 gcgccatctt gtggctgatg gtgccgacca cgaacagcac gtcggcctgg cgcggcgaga
     4201 agcgcggcag accggcgccg aagcggtcga tgtcgtagtg ggaacacgag gtggccatgt
     4261 actccatgcc acagcaggcc gtcacgaacg ggtactggaa gatggagtac ttccgtgccc
     4321 acccgatggc ctcgtcgagc ttgctggtga agaagccgcc tactcccact cgaacccccg
     4381 cttccgccag atgtacagga accccagcat gaggacgccc atgaagacca tcatctcggc
     4441 gaacccgaac cacccgagcc cccggtagag catagcccac ggccagatga agagcagctc
     4501 gatgtcgatc aggatgaaga agatggcgat cgtcgagaac ttgatcgcga agcgcccctc
     4561 ggagatttcg agcgggtcct tgccgcactc gaagggctcg accttcaccg gcgtgagccg
     4621 ccggggcgcg atcagcagcg ggatgcccag cagggcgccg gcgacgacgg cggcgaagcc
     4681 gaacaccatg aggatcgtcg cgtactcggt ggtcacgcgg ggtgctcggc cgcctgggtc
     4741 tgccgcctcg cgtggtacga gctgcgcacg agcggtcccg cctccacgtg ggcgaagccg
     4801 agcccgcggc cgatcgcggc cagggctgcg aactcgtccg gcgggtagta ccgctcgacc
     4861 ggcaggtgct gcgccgacgg ccgcaggtac tgcccgagcg tgaggatccg cacgtccgcc
     4921 gctcgcaggt cgcgcatcac cgcgagcagc tcctcgggcg tctcgcccag tccgaggatg
     4981 aggcccgact tggtgacgag gtccggcgcc gccgcccgcg cgcgacggaa cagctcgagc
     5041 acgcggtcgt agcggcctcc gtggcgggcg accttgtaga ggcggggcac cgtctccgtg
     5101 ttgtggttga gcacgtcggg gcgcgcgtcg atcaccgctg ccagcgcctc ggcctgcccc
     5161 tggaagtcgg ggatcagcgc ctccacacgg cacgccggcg cgtggcgccg taccgcccgg
     5221 atcgtggccg cccagtgcgc ggcgccgccg tcggcgagat cgtcgcgatt caccgacgtc
     5281 acgacgacgt agtcgagccc cagggcggcg acggcgcggc cgatccgctc gggctcctca
     5341 cggtcctccc aggcggggcg gccgtgggcg atcgcgcagt atccacaatt gcgggtgcag
     5401 atgtccccca ggatcatgaa cgtcgccgtc gcgtcctccc agcactcgcc gatgttgggg
     5461 cagcgcgcct cctcgcacac cgagtgcagg ttccactcgc gca
//
LOCUS       DATIAZ010000297         7117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1531000, whole genome shotgun sequence.
ACCESSION   DATIAZ010000297 DATIAZ010000000
VERSION     DATIAZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531000"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VLF19_10360"
     CDS             complement(<1..285)
                     /locus_tag="VLF19_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HSE93698.1"
                     /translation="MADFVMPILGADMTAGTLLAWRKQPGDRVARGDIIAEVETDKGL
                     IDVEVFASGVIERILVPPGTTVPTGTVLAVIHEDVPAAAAPSRAAPVTPAP"
     gene            complement(246..1262)
                     /locus_tag="VLF19_10365"
     CDS             complement(246..1262)
                     /locus_tag="VLF19_10365"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HSE93699.1"
                     /translation="MTKMTYREAARSALREALRTDARVFLMGEDVGRYGGAFAVSRGL
                     LEEFGPERVRDTPLSENAFLGAGIGAALGGLRPIVEVMTVNFSLLALDQVVNNAATLR
                     HMSGGQMSVPLVVRMATGGGRQLAAQHSHSLEGWYAHIPGIKVVAPGTPDDARALLLA
                     ALREPDPVFVFEHAFLYPLEGEVDETAGPGQIGCAAVRRDGRDLSLVAYGGSVPKALQ
                     AADVLAAEGIEAEVVDLRSLRPLDTAAVLASLSRTHRALIVDEGWRSGSFAAEVSARI
                     MEGGFYDLDGPVQRVCGAEVPIPYAGHLRGGGAAAGRHHRRRCPGDAPARWLTSSCRF
                     SAPT"
     gene            complement(1259..2191)
                     /gene="pdhA"
                     /locus_tag="VLF19_10370"
     CDS             complement(1259..2191)
                     /gene="pdhA"
                     /locus_tag="VLF19_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HSE93700.1"
                     /translation="MVLIRRFEEKAAELYSVGKIRGFLHLYIGEEAVAVGAMQALTPD
                     DNVVATYREHGQALARGVPAGAVMAEMFGKASGCSRGRGGSMHLFDVSRRFWGGHAIV
                     GGGLPVAVGLALADRMRGRAAVTACFFGDGAAAEGVFYESLNLAALWRLPVLFLCENN
                     LYAMGTALERHQAQTDIVRKGAAAGVPGEAVDGMDVVAVETAARRAAEAVRHGEGPFL
                     LEARTYRFRAHSMYDPDLYRGKEEIERWKTRDPITLFTAALGAAGLVAEGELVDLEAS
                     VAREVEAAVAEAEAGPWEPVEGLLDDVYTGPDGP"
     gene            complement(2266..2757)
                     /locus_tag="VLF19_10375"
     CDS             complement(2266..2757)
                     /locus_tag="VLF19_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE93701.1"
                     /translation="MTPPPARRRARAGAIVHPTDFSPASRPAFRAALALARHGRATLL
                     LVHVVAPMIQVPDVAVVSPPTYARFLDAAETAARRRLQRLVRAARRAGVRAAGLVVHG
                     VPHEEIVRVSRRRRARVVVIGTHGLTGFRRALLGSVAARVVALAPCPVLTVRAALGRT
                     AAA"
     gene            complement(2754..4520)
                     /gene="acsA"
                     /locus_tag="VLF19_10380"
     CDS             complement(2754..4520)
                     /gene="acsA"
                     /locus_tag="VLF19_10380"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSE93702.1"
                     /translation="MSWAPIVKHRGADAVAPSLDDYGRVSAAFTWAGARAELDGLPGG
                     RGLNIAHEAVDRHATGPRGDRIAIRWLGKGGAMRDYTYARLRELTDRFANVLDGLGVR
                     RGERVFALAGRIPELYVAALGTLKHGSVFCPLFSAFGPEPIRARMTIGQARVLVTTEA
                     LYRRKIVPLRPQLPDLAHVLLVGDADAPARVVDTLDFHALLDAAASRFSIPPTAPEDM
                     ALLHFTSGTTGRPKGAVHVHEAVVAHHVTGKYALDLHPDDVFWCTADPGWVTGTSYGI
                     IAPLTNGVTSVVDEAEFDAERWYRTLQDEHVSVWYTAPTAIRMMMKAGTDLVRKYDTS
                     ALRFLASVGEPLNPEAVVWGQEAFGRPFHDNWWQTETGGIMIANFAAMDIRPGSMGRP
                     LPGVAAAIVRKVGDERVEVIGEPDVQGELALRPGWPSMFRDYLGEPARYRKCFAGGWY
                     LTGDLARRDADGYFWFVGRADDVIKTSGHLVGPFEVESVLMEHPAVAEAGVIGKPDPV
                     AMEVVKAFVSLKVGRAPTPELKRELLAHARARLGAVVAPKEIDFAPSLPKTRSGKIMR
                     RLLKARELGLPEGDISTLEEPE"
     gene            5084..>7117
                     /locus_tag="VLF19_10385"
     CDS             5084..>7117
                     /locus_tag="VLF19_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93703.1"
                     /translation="MAEATGPPSTEQQVLGALEGLVAELGTTPPRGRVTLDDALDRDL
                     GLGSLERVELLFRLEQAFGIRFADAVLGEAETARDLVAAVRVGGASPAEAAVEPVAPA
                     EAGGPAPVTAPTLVDVLRWHAERAPERVHVVLRLEDGGEHRITHGALWTDARAIAAGL
                     RERGVAPGSSVALMLRTEPAFFATFFGVLVAGAVPVPIYPPFRADRLEEYAARQAKIL
                     DNAEARLLVTFREVERLAGLLRSRVRSLAGVTTPDRLAAPRLAPVPALHEEDAALIQY
                     TSGSTGDPKGVLLTHANLLANIRALGRALGVRPDDVMVSWLPLYHDMGLIGAWLGSLY
                     HAVPVVIMSPLAFLARPARWLRAISAHRATISAAPNFAFDLCVKKVADEELAGVDLGC
                     WRLAMNGSEAVSAETIERFTRRFAAKGFRAEAMCPVYGLAEASVGLTTSIPGQGARVD
                     RISRETFERTRRAEPARAGERAPLAVVSCGVPLPRHEVRIVDATGTPVPDRVEGHVEF
                     RGPSVTDGYFRNPAATAAVVRDGWMDSGDLGYRARGELYVTGRRKDLVIKAGRNVHPQ
                     EVEEVVGEVPGVRKGCVAAFGVGDPATGTERLVLVVETRATAPEARARLEAAVRERVV
                     DALGLPPDLVELAAPGSVLKTSSGKIRRSATREAWSRGELGRRASPRMQWMRLA"
BASE COUNT          945 a         2627 c         2590 g          955 t
ORIGIN      
        1 cggagccggc gtgacgggcg ccgcgcgcga gggcgcggcg gccgcgggca cgtcttcgtg
       61 gatgacggcc agcaccgttc cggtcgggac cgtcgtgccg ggcgggacga ggatgcgctc
      121 gatcaccccg ctcgcgaaga cctcgacgtc gatcaggccc ttgtcggtct cgacctccgc
      181 gatgatgtcg ccgcgcgcca cgcggtcgcc cggctgcttg cgccaggcga gcagcgtgcc
      241 cgccgtcatg tcggcgccga gaatcggcat gacgaagtca gccatcgcgc cggagcatcg
      301 cccgggcagc ggcggcgatg gtggcggcct gcggcagcgc cgcctcctcg gaggtggccg
      361 gcgtacggta tcgggacctc ggcgccgcac acgcgctgca cgggaccgtc caggtcgtag
      421 aacccgccct ccatgatcct ggcgctcacc tcggccgcga aactaccgct gcgccagccc
      481 tcgtcgacga tcagcgcccg atgggtgcgc gacagcgacg cgaggacggc ggccgtgtcg
      541 agcggccgca gcgaacgcag gtcgacgacc tcggcctcga tgccctcggc cgccagcacg
      601 tcggcggcct gcagcgcctt cggcaccgag ccgccgtacg cgacgaggct caggtcacgc
      661 ccgtcgcgac gcaccgccgc gcagccgatc tggcccgggc ccgccgtctc gtccacctcg
      721 ccctcgagcg gatagaggaa cgcgtgctcg aagacgaaga ccggatcggg ctcgcgcagc
      781 gccgccagca gcagcgcgcg cgcgtcgtcg ggcgtgcccg gcgccaccac cttgatgccg
      841 gggatgtggg cgtaccagcc ttcgaggctg tgcgagtgct gggcggcgag ctggcgcccg
      901 ccgcccgtcg ccatgcggac gacgagcggc acggacatct gcccgcccga catgtggcgc
      961 agggtggcgg cgttgttgac gacctggtcg agggccagca ggctgaagtt gaccgtcatc
     1021 acctcgacga tgggccgcag gccgcccagg gccgcgccga tacccgcgcc caggaacgcg
     1081 ttctcgctga gcggcgtgtc gcgcacccgc tccggcccga actcctccag gagcccccgg
     1141 ctcaccgcga aggccccgcc gtagcgcccc acgtcctcgc ccatcaggaa gacgcgcgcg
     1201 tcggtccgca gcgcttcccg cagcgcgctc cgcgccgcct cgcggtaggt catcttggtc
     1261 atgggccgtc cggccccgtg tagacgtcgt cgaggaggcc ctcgacgggc tcccacggcc
     1321 ccgcctcggc ctcggcgact gccgcctcga cctcgcgagc gaccgaggcc tcgagatcca
     1381 ccagctcgcc ctccgcgacg aggcccgccg cgcccagcgc ggccgtgaag agcgtgatcg
     1441 ggtcgcgcgt cttccagcgc tcgatctcct ccttgccgcg gtagaggtcg gggtcgtaca
     1501 tcgagtgcgc acggaagcgg taggtgcggg cctcgagcag gaaagggccc tcgccgtgcc
     1561 gtaccgcctc cgccgcgcgg cgcgccgccg tctccacggc cacgacgtcc atgccgtcca
     1621 cggcctcgcc cggcacgccg gccgccgcgc ccttccgcac gatgtccgtc tgcgcctggt
     1681 ggcgctcgag cgccgtgccc atggcgtaga ggttgttctc gcagaggaac agcacgggca
     1741 ggcgccagag cgcggcgagg ttcagcgact cgtagaacac gccctcggcg gcggcgccgt
     1801 cgccgaagaa gcaggcggtg accgcggcgc gcccccgcat ccggtcggcg agcgcgaggc
     1861 cgaccgcgac gggcagcccg cctccgacga tcgcgtggcc gccccagaaa cggcgggaga
     1921 cgtcgaagag gtgcatggac ccgccgcgcc cgcggctgca gccgctggcc ttgccgaaca
     1981 tctcggccat caccgcgccg gcggggacgc cgcgggccag ggcctggccg tgctcgcggt
     2041 acgtcgcgac cacgttgtcg tcgggggtga gcgcctgcat ggcgccgacg gccaccgcct
     2101 cctcgccgat gtagaggtgc aggaagccgc ggatcttgcc gacgctgtag agctccgccg
     2161 ccttctcctc gaagcggcgg atcagcacca tctggtgcag cagggcgagc gcgtgctcgc
     2221 gcgcgatccg cgtgttcaca cggcgatcgt cgccggtctg ggagatcatg ccgcggcggt
     2281 cctcccgagc gcggcgcgga ccgtgaggac cggacacggc gcgagcgcca cgacgcgcgc
     2341 ggcgacgctg cccaggagag cgcgccggaa accggtgagc ccgtgcgtcc cgatcaccac
     2401 gacccgcgcg cgccgccggc ggctcacccg cacgatctcc tcgtggggta cgccgtgcac
     2461 gacgagcccg gcagcgcgca cgccggctcg gcgggccgcc ctcaccaggc gctgcaggcg
     2521 ccttcgcgcg gccgtctcgg ccgcgtccag gaagcgcgcg tacgtgggcg gcgagacgac
     2581 cgcgacgtcc ggcacctgga tcatgggcgc caccacgtgg acgagcagca gcgtggcccg
     2641 gccgtgcctc gccagcgcga gcgccgcgcg gaatgctgga cgcgaggccg gcgagaagtc
     2701 ggtcgggtgc acgatcgcgc cggcacgggc gcgccggcga gcggggggcg gtgtcattcg
     2761 ggttcctcca gcgtcgagat gtcgccctcc ggcaggccca gctcgcgggc cttcagcagc
     2821 cggcgcatga tcttgccgct gcgcgtcttc ggcagcgagg gcgcgaagtc gatctccttg
     2881 ggcgccacga cggcgccgag gcgcgcgcgc gcgtgtgcga gcagctcgcg cttgagctcg
     2941 ggcgtcggcg cccggcccac cttgagcgag acgaacgcct tgacgacctc catcgccacg
     3001 gggtcgggct tgccgatcac tcccgcctcg gcgaccgcgg ggtgctccat cagcacgctc
     3061 tcgacctcga aggggcccac gaggtggccg gaggtcttga tcacgtcgtc ggcgcggccg
     3121 acgaaccaga agtagccgtc ggcgtcgcgc cgggcaaggt ccccggtcag gtaccagccg
     3181 ccggcgaagc acttgcgata gcgcgcgggc tcgccgaggt aatcacggaa catcgagggc
     3241 catcccggcc gcagcgccag ctcgccctgg acgtcgggct cgccgatcac ctcgacgcgc
     3301 tcgtcgccga ccttgcgcac gatcgccgcc gcgacgcccg gcagcggccg ccccatcgac
     3361 cccggtcgga tgtccatggc ggcgaagttc gcgatcatga tcccacccgt ctccgtctgc
     3421 caccagttgt cgtggaaggg gcggccgaag gcctcctggc cccagaccac ggcctcgggg
     3481 ttcagcggct cgccgacgct cgcgaggaag cggagcgcgc tcgtgtcgta cttgcgcacg
     3541 aggtcggtgc cggccttcat catcatgcgg atcgccgtcg gcgcggtgta ccagacgctg
     3601 acgtgctcgt cctgcagcgt ccggtaccac cgctcggcgt cgaactccgc ctcgtcgacg
     3661 acgctggtga cgccgttcgt gagcggcgcg atgatgccat aggacgttcc cgtgacccag
     3721 ccggggtcgg ccgtgcacca gaagacgtcg tcgggatgca gatcgagcgc gtacttgccc
     3781 gtgacgtgat gcgccaccac ggcctcgtgc acgtggacgg cgcccttggg cctgcccgtc
     3841 gtcccgctcg tgaagtggag cagcgccatg tcctcgggcg ccgtcggcgg gatcgagaac
     3901 cggctcgccg ccgcgtcgag gagcgcgtgg aagtcgaggg tgtccaccac ccgcgccggc
     3961 gcgtcagcgt cgcccacgag cagcacgtgc gccaggtcgg gaagctgcgg gcgcagcggc
     4021 acgatcttcc gccgatagag ggcctccgtc gtcacgagca cgcgagcctg cccgatcgtc
     4081 atgcgggcgc ggatgggctc ggggccgaag gccgagaaga gcgggcagaa gacgctgccg
     4141 tgcttgagcg tgccgagggc cgcgacgtag agctccggga tgcgaccggc gagcgcgaag
     4201 acccgctcgc cgcggcgcac accgaggccg tccagcacgt tggcgaagcg gtccgtcagc
     4261 tcgcggaggc gcgcataagt gtagtcgcgc atcgctccgc ccttgccgag ccaccggatg
     4321 gcgatccggt cccctcgggg gccggtcgcg tggcgatcga cggcctcgtg cgcgatgttc
     4381 agaccgcgcc cgcccggcag cccgtcgagc tccgcccggg ctccggccca cgtgaacgcc
     4441 gcggagaccc ggccataatc gtccaggctc ggggcgaccg cgtccgcgcc ccggtgcttg
     4501 acgatcggcg cccagctcag cgtcgcacct cctcaccagc ccggacccgt ccgcgcgtcg
     4561 aagacgactg cggtgcatcc gggatgccat acgttgtgct cgcgattccg tgcggttttg
     4621 acgccggcat gtggcgatgc gccgccccag gtggggcgcc gcctccacga gccgagcgcc
     4681 tccgggcggc gctctcgttc caccatgcgt ggcttctcac gccgttagca gggtgctgac
     4741 aaagtgattt cgaggtgctc gtcaacgtga ctccgacaac aggaaaatgc gaaaacattg
     4801 ggatttcgaa atatccgaag ctcggttccg tcacgtttag agggtctcaa aacactttgt
     4861 cagcagcctg ttagtggcgg cggcgcggct cttgctgtgg cttcaggccg cggttgccgt
     4921 ctgcccggga ggggctgaga tgatcggaga gatgtcgaga tgactcacgt ctccaccgcg
     4981 ctgtcgcagg agttgcggcg cccgccggcg gatgccgccg cctagagagt ctgccgtgac
     5041 cccggggccg gaggccctgg caaggggcca gaccgagacg accgtggccg aagccaccgg
     5101 ccctcccagc acggagcagc aagtcctcgg cgccctcgaa gggctcgtcg cggaactggg
     5161 cacgacgccg ccgcgcgggc gcgtcaccct cgacgacgcg ctcgatcgcg acctcggcct
     5221 cgggagtctc gagcgcgtcg agctgctctt ccggctggag caggcgttcg gcatccggtt
     5281 cgccgacgcg gtgctcggcg aggcggagac cgcgcgcgac ctcgtggccg ccgtgcgcgt
     5341 gggcggcgcg tcgcccgccg aggctgctgt cgagccggtc gcgccggcag aggctggcgg
     5401 cccggcgccc gtcaccgcgc cgacgctcgt cgacgtcctc cgctggcacg ccgagcgcgc
     5461 tcccgagcgc gtgcacgtcg tgctgcggct cgaggacggc ggcgagcacc ggatcaccca
     5521 cggcgccctg tggacagacg cacgggccat cgcggccggg ctgcgcgagc gcggcgtggc
     5581 acccggttcc tccgtggccc tcatgctccg gacggagccc gcattcttcg ccacgttctt
     5641 cggcgtgctc gtcgccgggg ccgtcccggt gccgatctat ccgccgttcc gggcggatcg
     5701 cctcgaggag tacgcggcgc gccaggcgaa aattctcgac aacgcggaag cgcggctgct
     5761 cgtcacgttc cgcgaggtcg agcggctggc cggactgctg cgctcgcgcg tgcgctccct
     5821 cgccggcgtg acgacacccg accgcctggc cgcgccgcgc ctcgctccgg tcccggccct
     5881 ccacgaggag gacgcggcgc tcatccagta cacgtcgggg agcaccggcg acccgaaggg
     5941 cgtgctgctc acgcacgcga acctgctcgc caatatccgg gcgctcggcc gggcgctcgg
     6001 cgtgcgcccc gacgacgtca tggtctcgtg gctgccgctc taccacgaca tgggcctcat
     6061 cggtgcctgg ctcggctcgc tctaccacgc cgtccccgtc gtcatcatgt cgccgctggc
     6121 gttcctggcc cgccccgcgc gctggctgcg cgccatctcc gcacaccggg cgaccatctc
     6181 ggcggcgccg aacttcgcct tcgacctctg cgtcaagaag gtcgcggacg aggagctggc
     6241 gggcgtggat ctcggctgct ggcggctggc gatgaacggc tccgaagccg tgagcgccga
     6301 gacgatcgag cgcttcacgc ggcggttcgc ggcgaaaggc ttccgcgccg aggcgatgtg
     6361 cccggtctac gggctggcgg aggcatcggt ggggctcacg acgtcgatcc ccgggcaagg
     6421 cgcgcgcgtc gatcggatca gccgcgagac gttcgagcgc acccggcgcg cggagccggc
     6481 ccgcgcgggc gagcgggcgc ccctggccgt cgtctcgtgc ggcgtgccgt tgccgcggca
     6541 cgaggtgcgc atcgtggacg cgacggggac gcccgtgccc gaccgggtcg aggggcacgt
     6601 ggagttccgc ggcccatcgg tgacggacgg ctacttccgc aaccccgcgg cgacggccgc
     6661 cgtcgttcgc gacggctgga tggactccgg ggatctcggg taccgcgcac ggggcgagct
     6721 ctacgtcacg ggccgccgca aggacctcgt catcaaggcc ggacgcaacg tgcatccgca
     6781 ggaggtggag gaggtggtgg gcgaggtgcc cggcgtgcgc aagggctgcg tcgcggcatt
     6841 cggcgtgggc gaccccgcca ccggcaccga gcggctcgtc ctggtggtgg agacgcgggc
     6901 gacggcgccc gaagcgcgcg cgcggctcga ggccgccgtg cgcgagcggg tcgtcgacgc
     6961 gctcgggttg ccgcccgatc tcgtcgagct cgccgctccc ggctccgtgc tgaagacctc
     7021 cagcggcaag atccggcgct cggcgacgcg cgaggcgtgg tcgcgtggcg agctcggcag
     7081 gcgcgcgtca ccgcggatgc agtggatgcg cctcgcg
//
LOCUS       DATIAZ010000298         7243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1531005, whole genome shotgun sequence.
ACCESSION   DATIAZ010000298 DATIAZ010000000
VERSION     DATIAZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531005"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..3480)
                     /locus_tag="VLF19_10390"
     CDS             complement(325..3480)
                     /locus_tag="VLF19_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSE93704.1"
                     /translation="MLSRYREPLRAWTDPIGHALFRLRLRPNHLTLAGLGVSLLAAGA
                     FVVGRTRIGGVLLILAGLFDFFDGSLARASGQVTPFGAFLDSVIDRYSDLVVLLGIVV
                     LFAGMPHARGAIIAMAGLVGSVMVSYTKARAESIGVECTVGMMERPERMICLIAGAVL
                     DLLEPALWVLAVLANFTALQRIAFTWRATRSATVLTFAGMLLLTASPASATAASEAPP
                     ERASPAQSGVTTLPPTPEGRDRVSAPAAASTAIPGPAGSPSPTQGAATAEPAQQATSA
                     PAASASPNPASAIPAEPATNPVSGTPAAENRTPTGPPPTSGAASEISPPAGSIPDTGI
                     PAETPGLPAPQPAGPSAPAASSPARTAPPAAAVTAPAVTPADRPIATTPAPAVAPETE
                     RAWARAIEAYAEGDPRPALREFATPAALGGPIGDHLGLLLADARERVGDLAGARAAAL
                     GVADRYRDSRLAPGALVLATTLAFRTRDEAAAQAVLVRLISSYPDAPETPGALYLLGM
                     TGEARGELAAAAAAYRELRVIAPASGWADGAEDRLALLTAAGVRVPELSVTQRLDRAE
                     RLLRGGVPKTASDEAERIVNDSRDPSVIARAFRVVADASARLGQYEVAARALEALAAR
                     SPAVRRPTLWLEQARLLVRAGRRDRALAVLTEVEARGAESEAAEATYLRGRVLDDLDR
                     QPQASTAYAAVASRHPNREVAGAALWRLGWLAHARNDMRAAERAWLKVTQISGGRGYR
                     VPALYWAGRMRERTSGRAAAADMYRKVLAEAPRSYYGTLAARRVAAPGPAGTGPPLKL
                     PADPRQALGDDPGFLRVELLRRIGLVEAAAQELDDVVLRSVGDPVRLYGLSGAFVRDE
                     RYHVALRILRRHFATVASTGHDALPRAFWEMLYPFPWRDEIAVAAQRNGLDPNLVAAV
                     VREESSYYPRAVSRTGARGLMQLMPATAQPMAAHRGLAFREGNLLDDPRANIQLGTAF
                     LAGLMREWNDARLALAAYNAGPRRVRDWWKARRTSDVEAFVELIPFDETRHYVKRVML
                     SWDEYRRLYEAAGGNTAAAPD"
     gene            complement(3500..6178)
                     /gene="glnD"
                     /locus_tag="VLF19_10395"
     CDS             complement(3500..6178)
                     /gene="glnD"
                     /locus_tag="VLF19_10395"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HSE93705.1"
                     /translation="MSPELYRRARRAEAKAELAEDRRRLRLECFRAALADGLDSLKAM
                     HAEGASGQASVQAHARLIDDLVRSLTRLIARDLAAAALTPAPFVVVALGGYGRGELHP
                     SSDIDLMVIHDGELGPYVQRVTQELLYTLWDLGLTIGHSLRSLEDCVAIARTDLPSRT
                     SMQEARFLAGDRRLFARFGKVFRENVYRRDFEQFLEATLLERDQRYRKHGGSPYVGEP
                     NVKESAGGLRDMHTAMWLSSAKFGARTLRELAHRGLITAREQQATDAALTFLWRVRNE
                     LHFLAGHKNDVLSRDLQPRVARNFDYENAGDTLAVERFMRDYYLHARVIHRVSRRLIA
                     RCGETLSPRDAAARRDRQQALADGLVFIDGKLYLADQDARVFADDPMRLIKVFWHAHR
                     LGCDLSLELERAIEESLDRLDDRVRRAPEARDLLLDVCRTWGRVAVTLGQMHELGLLG
                     RYLPEWGALTCLVQYDVYHKFSADRHSLLAVEHLEALAPGQSAESEGAAQVLAEVDKP
                     EVLMLGVLLHDIGKAKGHGHAAKGIPLIQALVARIGLSPEDAGAVEFLVAHHLTMSHI
                     AQRRDIDDPKTIADFAVTVGDPERLRMLHLLTWADMRAVGPGVLTPWQAMMLHELYAR
                     TLARLTGGRVERPNRAQLAERLRAEVEAEVPRQAVRGHLAMMSDRYLASTNVQRMADH
                     LRLLARLETAPVVTELFHHPDLGSSDLVVVTRDQPGLFALIAGTLAAHGVNIISAQIA
                     TRADGIAIDTFQVNDPAGEALTSPAHWSRALAALRTVLSGEQTVDALLERRRAGRRGP
                     AAVPSPPKITIDNSLSDEFTVVEVKCPDRLGLLHLVTRTLSALGLDIASARIATEIDQ
                     AFDTFYVHDGKGGKITTPEAMEGLREALEQALVQPL"
     gene            6248..>7243
                     /gene="hisC"
                     /locus_tag="VLF19_10400"
     CDS             6248..>7243
                     /gene="hisC"
                     /locus_tag="VLF19_10400"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSE93706.1"
                     /translation="MRPIWRPALDRITPYEAGPPLESLARDLGLADLVRLSANEHPTG
                     PSRRVVEAIAREASRAHLYPDGGSTALRAALAARLGVGSEQIVAGNGADELLALIAWA
                     AIDAGDEVVVPLPSFEPYTTVVALAGGVTVPSPLAKYDTDLDDMLRRVGPRTKALVIC
                     SPHNPAATIVRRAPLQALLDALAPDPPLVVLDEAYRDFCDDPDYPDGVELLRRHPRLI
                     VLRTFSKIAGLAGLRVGYAVGSAETIDRLNRVRAPYNVNRLGQVAALAALEDDAHREH
                     TRALVIAEREFLARELSRRGFAFPPSQANFLLVQVAEAAAARDRLLRAGVIVRDGT"
BASE COUNT         1000 a         2596 c         2694 g          953 t
ORIGIN      
        1 tgaaccaggc cgacggcatc cacccgaacg cggaaggcca ccggatcatc gcggacacta
       61 tctggacgta cctgaagccg ctgctcggag ctcccgcgcg gcgacccgcg acgttagccc
      121 cggggctggg cctcatgtcc ctcggaggcc gccggggttg accaggcgcg acacggctac
      181 gccgggtcgt gccgagccgc ccgggggggg ttggccccca tgttcaggcg cgagccgagc
      241 gagagaccga ggcgcgacac ggctacgccg ggtcgtgccg agccgcccgg ggggcttggg
      301 ggccctgtcg gggcccccat gtcattagtc gggcgcagca gccgtgttcc cgccggccgc
      361 ttcgtagagc cggcggtact cgtcccagga gagcatcacg cgcttgacgt agtggcgcgt
      421 ctcgtcgaag gggatcagct ccacgaacgc ctcgacgtcg ctcgtccgcc gcgccttcca
      481 ccagtcgcgc acgcgccggg ggcccgcgtt gtacgcggcg agggccaagc gcgcgtcgtt
      541 ccactcgcgc atgagaccgg cgaggaacgc ggtaccgagc tggatgtttg cgcgcgggtc
      601 gtcgagcagg ttgccctcgc ggaacgccag gccccgatgc gctgccatcg gctgcgccgt
      661 cgccggcatg agctgcatga ggcctcgcgc gcccgtccgc gacaccgcgc gggggtaata
      721 gctcgactcc tcgcggacga ccgcggcgac caggttcggg tcgagcccgt tgcgctgggc
      781 cgccaccgcg atctcgtcgc gccacgggaa cggatagagc atctcccaga aggcgcgcgg
      841 cagcgcgtcg tgcccggtgg acgccaccgt ggcgaagtga cggcgcagga tgcgcagcgc
      901 gacgtgatag cgctcgtcgc gcacaaaggc gccggagaga ccgtagaggc gcacggggtc
      961 gccgaccgag cgcagcacga cgtcgtccag ctcctgggcg gcagcctcga cgagcccgat
     1021 acgccggagc agctcgacgc gtaagaagcc ggggtcgtca cccagcgcct gccgcgggtc
     1081 ggcaggtagc ttgagcgggg gaccggtccc cgccggtcca ggcgcggcaa cacggcgcgc
     1141 ggcgagcgtg ccgtagtagc tgcgcggcgc ctcggcgagg accttgcgat acatgtcggc
     1201 cgccgcagcg cgacccgacg tccgctcgcg catgcgtccc gcccagtaga gggcgggaac
     1261 acgatacccg cgaccaccgg aaatctgggt caccttgagc cacgcgcgct ccgcggcgcg
     1321 catgtcattc ctcgcgtggg cgagccagcc cagacgccag agcgccgcgc cggcgacctc
     1381 gcggttggga tggcgcgagg ccacggcggc gtaggccgtc gaggcctgcg gctggcgatc
     1441 gaggtcgtcg agcacccgcc cccgcagata ggtcgcctcc gccgcctcgg actcggcgcc
     1501 gcgggcctcg acctcggtga gcacggcgag ggcgcggtcc cgccggccgg cgcgcacgag
     1561 caggcgcgcc tgctcgagcc atagcgtcgg ccggcggacg gccggcgagc gcgccgccag
     1621 cgcctccagg gcgcgggcgg cgacttcgta ctgtcccaga cgcgcggagg cgtcggcgac
     1681 cacacggaac gcccgggcga tgacggaggg atcgcgcgaa tcgttgacga tgcgctcggc
     1741 ctcgtcggac gcggtcttgg gcacgccgcc gcgcagcagc cgctcggcgc ggtccagtcg
     1801 ctgcgtcacg gacagctcgg gcacgcgcac gccggcggcg gtgagcaggg cgaggcggtc
     1861 ctcagcaccg tccgcccagc cgctcgccgg cgcgatgacc cggagctcac gataggcggc
     1921 tgccgcggcg gcgagttcgc cccgcgcctc tcccgtcata ccgagcaggt agagcgctcc
     1981 cggggtctcc ggcgcgtccg ggtacgagga gatcagccgg acgagcacgg cctgcgccgc
     2041 cgcctcgtcg cgcgtccgga acgccagcgt cgtggccaac acgagcgctc ccggcgccag
     2101 ccggctgtcg cggtagcggt cggcaacgcc gagcgccgcg gcgcgcgccc cggcgagatc
     2161 gcccactcgc tcgcgggcgt cggccagcaa caggcccagg tgatcgccga tgggaccccc
     2221 gagcgcggcc ggcgtcgcga actcgcgcag cgccgggcgc ggatcgccct cggcatacgc
     2281 ctcgatcgcc cgcgcccatg cgcgttcggt ctccggtgca actgccggcg caggggtcgt
     2341 ggcaatcggc ctgtcggctg gcgtcaccgc cggcgccgtt acggctgcgg ccggcggggc
     2401 cgtcctggcc ggggatgaag cggccggggc cgaaggccca gccggttgcg gcgcgggcaa
     2461 acccggagtc tcggccggga tccccgtgtc cgggatcgac cccgccgggg gcgatatctc
     2521 ggaggctgct cccgaagtcg ggggcggtcc tgtcggggtt cggttctcgg ccgccggcgt
     2581 gccagaaacc gggttcgtgg ccggctcggc tggaatcgcc gaggccgggt tcggcgaggc
     2641 cgaagcggcc ggagcggacg tcgcctgctg ggccggctcg gccgtcgcgg caccctgggt
     2701 gggcgacggt gacccggccg gccccgggat cgcggtcgaa gcggcggctg gggccgaaac
     2761 gcggtctcga ccctctggcg tcggtggcaa tgtcgtcacg ccgctctggg ccggcgaggc
     2821 cctctccggc ggcgcctcgg aggcggcggt cgcggaagcg ggcgatgcgg tgaggagcaa
     2881 catgccggcg aaggtcagca cggtggcgct ccgtgtcgcc cgccacgtga aggcgatccg
     2941 ctggagcgcc gtgaagttgg cgaggacggc caggacccag agcgccggct ccagcagatc
     3001 gagcaccgca cccgcgatca ggcagatcat gcgctcgggc cgctccatca tgccgaccgt
     3061 gcactccacg ccgatcgact cggcgcgggc cttggtgtag ctgaccatca cgctgccgac
     3121 gagaccggcc atggcgatga tcgcaccgcg tgcgtggggc atgcccgcga agagcacgac
     3181 gatgccgagc agcacgacga gatcggagta gcggtcgatc accgagtcga ggaacgcgcc
     3241 gaacggcgtc acctggcccg aggcacgggc cagcgagccg tcgaagaagt cgaagagccc
     3301 ggcgaggatc agcagcacgc cgccgatgcg ggtgcggccg accacgaagg cgccggcggc
     3361 gagcaggctc acgccgaggc cggcgagcgt cagatggttc ggccggagcc gcaggcggaa
     3421 cagcgcatgg ccgatggggt cggtccacgc gcgcagcggc tcgcgatacc ggctcagcac
     3481 tgcggtcgat tcccggtcgt cacagcggct ggaccagcgc ctgctcgagc gcctcgcgga
     3541 gcccttccat cgcctcgggc gtcgtgatct tgccgccctt gccgtcgtgc acgtagaagg
     3601 tgtcgaaggc ctggtcgatc tcggtggcga tgcgggcgct ggcgatgtcg agccccagcg
     3661 cggagagcgt gcgcgtgacg aggtggagca ggccgaggcg gtcggggcac ttcacctcca
     3721 ccaccgtgaa ctcgtcgctg aggctgttgt cgatggtgat cttggggggc gacggcaccg
     3781 ccgccggacc gcgccgcccg gcgcgccggc gctcgagcag cgcgtccacg gtctgctcgc
     3841 cggagagcac ggtgcgcagc gccgcgagcg cccggctcca gtgcgccggt gacgtgagcg
     3901 cctccccggc gggatcgttc acctggaacg tgtcgatggc gataccgtcc gcgcgggtgg
     3961 cgatctgggc ggagatgatg ttcacgccgt gggccgccag cgtgccggcg atgagcgcga
     4021 agagcccggg ctgatcccgc gtgaccacga ccaggtcgga cgagccgagg tccggatggt
     4081 ggaacagctc cgtcaccacc ggcgccgtct cgagccgcgc gagcaggcgc aggtggtcgg
     4141 ccatccgctg cacgttggtg ctggcgaggt agcggtcgga catcatcgcc aggtggcccc
     4201 tgacggcctg ccgcgggacc tcggcctcga cctccgcccg cagccgctcg gcgagctggg
     4261 cgcggttggg acgctcgacg cgcccgccgg tgaggcgcgc gagcgtccgc gcgtacagct
     4321 cgtgcagcat catcgcctgc cacggcgtca gcacgccggg cccgaccgca cgcatgtcgg
     4381 cccaggtcag gaggtggagc atccggagcc gctccgggtc gcccaccgtc accgcgaagt
     4441 cggcgatggt cttggggtcg tcgatgtcac gacgctgggc gatgtgcgac atcgtcaggt
     4501 gatgggccac caggaactcc acggcgccgg catcctcggg cgacagcccg atgcgcgcga
     4561 ccagcgcctg gatcagcggg atgcccttgg ccgcgtggcc gtgccccttg gccttgccga
     4621 tgtcgtgcag gagcacgccg agcatcagca cctcgggctt gtcgacctcg gccaggacct
     4681 gggccgcgcc ctccgactcc gccgactgtc ccggcgcgag cgcctcgaga tgctcgacgg
     4741 cgagcagcga gtgccggtcg gcggaaaact tgtgatagac gtcgtactgc acgaggcagg
     4801 tgagcgcgcc ccattccggc aggtagcggc ccagcaaccc cagctcgtgc atctgaccga
     4861 gcgtcaccgc cacgcggccc caggtgcggc agacgtcgag cagcaggtcg cgcgcctcgg
     4921 gcgctcgccg cacgcggtcg tccagccggt ccagcgactc ctcgatcgcg cgctcgagct
     4981 ccaggctgag gtcgcagccc agccggtgcg cgtgccagaa caccttgatc aggcgcatcg
     5041 gatcgtcggc gaacacgcgc gcgtcctggt cggcgagata caacttaccg tcgatgaaga
     5101 cgagcccgtc ggccagcgcc tgctggcggt cgcgccgcgc cgccgcgtct cgaggcgaga
     5161 gcgtctcacc gcatcgtgca atgagccgcc gcgacacgcg gtggatgacg cgggcgtgca
     5221 ggtagtagtc gcgcatgaac cgctcgaccg cgagggtgtc acccgcgttc tcgtagtcga
     5281 agttccgggc gacccgcggc tgcaggtcgc gcgagagcac gtcgttcttg tggccggcga
     5341 ggaagtgcag ctcgttgcgg acgcgccaga ggaacgtcag cgcggcgtcc gtggcctgct
     5401 gctcgcgcgc ggtgatgagg ccgcggtggg ccagttcgcg cagcgtccgg gcgccgaact
     5461 tcgccgagct cagccacatg gcggtgtgca tgtcgcgcag gccgcccgcc gactccttga
     5521 cgttgggttc gccgacgtag ggcgagcccc cgtgcttccg gtagcgctgg tcgcgctcga
     5581 ggagcgtcgc ctcgaggaac tgctcgaagt cgcggcggta gacgttctcg cggaacacct
     5641 tgccgaagcg cgcgaacagc cgccggtcgc cggcgaggaa ccgggcctcc tgcatggagg
     5701 tgcggctcgg caggtcggtg cgagcgatgg ccacgcagtc ctccagagac cgcaggctgt
     5761 ggccgatggt gaggccgagg tcccacagcg tgtagagcag ctcctgggtc acccgctgca
     5821 cgtagggtcc gagctcgccg tcgtggatca ccatgaggtc gatgtcggac gaggggtgca
     5881 gctcgccgcg gccgtaaccg ccgagcgcga ccaccacgaa cggcgccggc gtgagcgccg
     5941 cggcggcgag gtccctcgcg atcagacggg tgagcgagcg caccagatcg tcgatgagcc
     6001 gcgcgtgggc ctggacggac gcctgccccg acgccccttc cgcgtgcatc gccttcagag
     6061 agtcgagccc gtcggccagt gcggcgcgga agcactccag gcgcagccgc cgccggtcct
     6121 ccgcgagctc ggccttggcc tcggcacggc gggcccggcg gtacagctcg ggcgacatga
     6181 cgacgcagag cgtagcaacc ccgcctgaac actgtcaaat gcgctgtggt ctgactacaa
     6241 tgcccgcgtg cgcccgatct ggcgtccggc gctcgaccgc atcacgccct acgaggcggg
     6301 gcctccgctg gagtcgctcg cccgggatct cgggctcgcc gatctcgtcc ggctgtcggc
     6361 gaacgagcac ccgacgggcc cctcgcgccg cgtcgtggag gcgatcgcgc gtgaggcgtc
     6421 ccgcgcgcac ctctaccctg acggcggctc cacggcgcta cgggccgcgc tggcggcgcg
     6481 gctcggcgtg gggtcggagc agatcgtcgc cggcaacggc gcggacgagc tactggctct
     6541 catcgcgtgg gccgccatcg acgccggcga cgaggtggtc gtgccgttgc cgtccttcga
     6601 gccctacacg accgtggtcg ctctcgccgg cggcgtcacc gtcccgagcc cgctggccaa
     6661 gtacgacacc gacctcgacg acatgctccg gcgtgtcggc ccgcggacca aggcgctggt
     6721 catctgctcg ccgcataatc ccgcggcgac catcgtgcgg cgcgcgccgc ttcaggcgct
     6781 gctcgacgcg ctcgccccgg accctccgct cgtggtcctg gacgaggcgt accgggattt
     6841 ctgcgacgat cccgactatc cggacggcgt cgagctgctc cggcgccatc cccggctgat
     6901 cgtcctgcgg accttctcga agatcgcggg gctcgcgggc ctgcgcgtgg gctacgccgt
     6961 ggggagcgcc gagaccatcg accgcctcaa ccgtgtgcgc gcgccctaca acgtgaaccg
     7021 gctcggccag gtcgccgcgc tggccgcgct cgaggacgac gctcaccgcg agcacacgcg
     7081 tgcgctcgtc atcgccgagc gtgagttcct cgcgcgcgag ctgagtcggc ggggcttcgc
     7141 gtttcctccg tcgcaggcca atttcctgct cgtgcaggtc gccgaggcgg cagcggcccg
     7201 cgatcggctg ctgcgcgcgg gcgtgatcgt ccgcgacggc aca
//
LOCUS       DATIAZ010000299         4455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_612236, whole genome shotgun sequence.
ACCESSION   DATIAZ010000299 DATIAZ010000000
VERSION     DATIAZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612236"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="VLF19_10405"
     CDS             <1..1355
                     /locus_tag="VLF19_10405"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSE93707.1"
                     /translation="GFVEAYGRACPGEVVRITEPVGIEHDVMAVVLEYERRRRHPVLV
                     FERVRGHDVPIVCNVVASRRALAFALGVPERDLAAEYARRIKERIKPVVVADPPFRHG
                     VLTGDAVDLGALPIPEYFPGDAGRYLTAGMLVARDPDTGIETEGYHRFQVKGRDRMGV
                     SLHSRRRMFEYQRRAEARGRPLPCAIVLGLHPLVSMGSLAYPPPDVGKFEVVGGLLGE
                     PLQTSPCGTVDLHVPATAEIVIEGEILPGVREPEGPFGEFTGYFSRRSTEHVFVARAI
                     AMRERPWFQSIGSGRAGDHITTLGLIREAEIANALGRAIPNVTGVHVPLSGTSSFTAY
                     VAIKQGRPGEAKHVIPIVLGVDHYLKLVVVVDDDIDVFDESDVMWAVATRVQADRDLV
                     VISGSLGAMLDPSADERGVTAKLGIDATRPFGQPFAEKLVMSRERMAWARALVDTLSS
                     "
     gene            1366..2034
                     /locus_tag="VLF19_10410"
     CDS             1366..2034
                     /locus_tag="VLF19_10410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSE93708.1"
                     /translation="MPKRLIDLSVTVDDRTMSPPSTNMRLEITPHRRGPGFWQVSSVH
                     QSLHTGAHVDSPLHVFKDGITTAEIALDQVTGEAVVVDLSFVGANRGVTIDDLRRGGA
                     DDVRAGDIVLLRTDWTDKMYGKWPDYFTQSPFFPPESAEWLVAKGAKNIGFDFFEEYC
                     ARLPDFTSEDFPMHRVILGAGVVIMEGLTNLGALPRRRVDFYAPFFKIAGTEGAPARF
                     FAEV"
     gene            complement(2148..3128)
                     /locus_tag="VLF19_10415"
     CDS             complement(2148..3128)
                     /locus_tag="VLF19_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSE93709.1"
                     /translation="MDARGVVVLGCGWIARRHAAAARGLGIPLVFASRDIGRARDYAR
                     RFRAIAAYGSYDEALRDPRASGAVVCTPHDRHLADVQRALDAGKHVLVEKPIARTLPE
                     ADAMIDAAAKAGRVLMIAEQFHFMPAFRRVRAVLEDGRLGGLRDILLVARGLHHRSGW
                     RLDAAAAGGGALIDGGIHYVHNLRWWGGPVRRLFALRPPQTVHSLAGEDGVSLLAELA
                     GGAVGMLANSLGSPGLPKLQWSTVTGARATCFADNRGRWVALRGDGRFRLWLFRRDTR
                     GYGAMLRAFRDAMERGRAEEMDGVAGRADLAVVLAAYRSIAEHGPVEVAC"
     gene            3178..4332
                     /locus_tag="VLF19_10420"
     CDS             3178..4332
                     /locus_tag="VLF19_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HSE93710.1"
                     /translation="MRRVAVIGVGVTRFGKHDRTSAELFAEAAREALADAEIEPRAVQ
                     ALYYGNVVGGETEKQLHTGPQAASVLGIPTVPTTRFETACASSHAAFRHAVMEIAAGV
                     SDVVLVGGAERVLNVPTADSTEYFAYASDASWEQPAGLTFPGVFALVARAHMTKHGTT
                     EEQMAHVAVKNHRHGALNPKAQFQKEITLQQVLASAYVADPLKLYDCCPFTDGGAAVV
                     LASEEVARTRRRAVWVLGSAAASDAMLLHDKRDLARIPATERAAAAAYRQAGLGPRDV
                     NVVELHDCFTIAEIVATEGLGLFEPGTGGAAAEKGWTSLGGKIPVNTSGGLKAKGHPI
                     GATGAAQVVEVVTQLRGEAGARQVDGARIGLTHTLGGNTATVLVSLFGRD"
BASE COUNT          645 a         1626 c         1570 g          614 t
ORIGIN      
        1 gaggcttcgt cgaggcgtac ggacgcgcgt gtcccggcga ggtcgtgcgc atcaccgagc
       61 cggtggggat cgagcacgac gtgatggcgg tcgtgctcga gtacgagcgg cgccgccgcc
      121 atccggtgct cgtcttcgag cgcgtgcgcg gccacgacgt ccccatcgtc tgcaacgtcg
      181 tggcgagccg gcgcgcgctg gccttcgcgc tgggggtgcc cgaacgcgat ctcgccgcgg
      241 agtacgcccg gcgcatcaag gagcgcatca agccggtcgt cgtggctgac ccgcccttcc
      301 gccacggcgt gctcaccggc gacgccgtcg atctcggggc gctgccgatc cccgagtact
      361 tcccgggcga cgccggccgc tacctcaccg cgggcatgct cgtggcccgc gaccccgaca
      421 cgggcatcga gaccgagggc taccaccgct tccaggtcaa gggccgcgac cgcatgggcg
      481 tgagcctgca ctcgcgccgc cgcatgttcg agtaccagcg tcgcgcggaa gcccggggcc
      541 ggccgctgcc gtgcgcgatc gtgctggggc tgcacccgct ggtgtcgatg ggctccctcg
      601 cctacccgcc gcccgacgtg ggcaagttcg aggtcgtcgg cggcctgctg ggtgagcctc
      661 tccagacttc tccgtgcggt accgtggacc tgcacgtgcc agccacggcc gagatcgtca
      721 tcgaggggga gatcctgccc ggtgtgcgcg agcccgaagg cccgttcggc gagttcaccg
      781 gctacttctc ccgacgctcg acggagcacg tcttcgtcgc cagggccatc gccatgcgtg
      841 agcggccctg gttccagtcg atcggctccg gccgggccgg cgaccacatc accacgctcg
      901 ggctgatccg cgaggccgag atcgccaacg cactgggccg cgcgatcccg aacgtcaccg
      961 gcgttcacgt gccgctcagc gggacgtcgt ccttcaccgc ctacgtggcc atcaagcagg
     1021 gccggcccgg cgaggccaag cacgtcatcc ccatcgtgct cggcgtggac cactacctga
     1081 agctcgtcgt cgtcgtggac gacgacatcg acgtcttcga cgagtccgac gtcatgtggg
     1141 ccgtggccac gcgcgtgcag gccgaccgcg atctcgtcgt gatctcgggc agcctcggcg
     1201 cgatgctcga ccccagcgcc gacgagcgcg gcgtcaccgc caagctcggc atcgacgcca
     1261 cccgtccctt cggccagccc ttcgccgaga agctcgtgat gtcgcgcgag cggatggcct
     1321 gggcgcgggc gctcgtcgac acgctcagct cctgagagga ccgccatgcc caagcgcctg
     1381 atcgacctgt cggtgaccgt ggacgaccgc accatgagcc ccccctcgac caacatgcgg
     1441 ctcgagatca cgccgcaccg ccgcgggccg ggcttctggc aggtctccag cgtgcaccag
     1501 agcctgcaca ccggagccca cgtcgactcg cccctgcacg tgttcaagga cggcatcacg
     1561 accgccgaga tcgcgctgga ccaggtgacc ggcgaggccg tcgtcgtcga cctgtcgttc
     1621 gtcggcgcca accgcggcgt gacgatcgac gacctgcggc gcggcggtgc cgacgacgtc
     1681 cgggccggcg acatcgtgct gctgcgtacg gactggaccg acaaaatgta cggcaagtgg
     1741 cccgactact tcacgcagtc gccgttcttc ccgccggagt ccgccgagtg gctcgtggcc
     1801 aagggggcca agaacatcgg cttcgacttc ttcgaggagt actgcgcgcg gctgcccgac
     1861 ttcacctccg aggactttcc catgcaccgg gtgatcctcg gggcgggcgt cgtgatcatg
     1921 gaagggttga cgaacctcgg cgcgctgccg cgccggcgcg tggacttcta cgcgccgttc
     1981 ttcaagatcg cggggaccga gggcgcgccg gcgcgcttct tcgccgaggt ctagcgggac
     2041 tgcccacggc accaggctgc ggcggatggc gctggctagt gggaggcggc cgcggtgaag
     2101 gtcggagcgg cgacgccgtc gatggggacg tcgacgcagg cagacactca gcatgcgacc
     2161 tcgacggggc cgtgctcggc gatggatcgg taggcggcca gcacgacggc gaggtcggcg
     2221 cgcccggcga cgccgtccat ctcctccgcc cggccccgct ccatcgcatc acggaacgcc
     2281 cgcagcatcg ccccgtagcc ccgagtgtcc ctccggaaga gccagagacg gaagcggccg
     2341 tctccgcgca gggcgaccca cctccctctg ttgtcggcga aacaggtcgc ccgcgcaccg
     2401 gtcactgtcg accattgaag cttcggaagg cccggagaac caagagagtt ggcgagcatg
     2461 cccaccgccc cgccggccag ctccgccagc aggctgacgc cgtcttcccc ggcgagcgag
     2521 tgcaccgtct gcggcggccg gagcgcgaac agccgacgca cgggaccgcc ccaccagcgg
     2581 aggttgtgga cgtagtgaat gccgccgtcg atgagcgctc cgccgccggc ggcggccgcg
     2641 tcgagacgcc agccgctgcg gtggtggagg ccccgcgcca cgagcaggat gtcgcggagg
     2701 ccgcccaggc ggccgtcctc gagcaccgcg cgcacgcgcc tgaaggccgg catgaagtgg
     2761 aactgctcgg cgatcatcag cacacgcccg gccttcgcgg cggcgtcgat catcgcgtcg
     2821 gcttccggga gcgtccgggc aatgggcttc tcgacgagga cgtgcttgcc cgcgtccagc
     2881 gcgcgctgca cgtcggcgag gtggcggtcg tgcggcgtgc acaccaccgc gccgctcgcg
     2941 cgcgggtcgc gcagcgcctc gtcgtaggag ccataggcgg cgatggcgcg gaaccggcgc
     3001 gcgtagtcgc gcgcgcggcc gatgtcgcgg ctggcgaaga cgagcgggat ccccagccct
     3061 cgggccgccg cagcgtggcg acgcgcgatc cagccgcagc cgagcacgac cacgccgcga
     3121 gcgtccatcg ccggatgcta tcattgtcgt cgcgtcccag gagcccgagg aggacctatg
     3181 cgtcgcgtcg cggtgatcgg ggtcggagtg accaggtttg gcaagcacga ccggacctcg
     3241 gcggagctgt tcgccgaggc ggcgcgcgag gccctggccg acgcggagat cgagccgcgc
     3301 gccgtgcaag cactctacta cggcaacgtc gtgggcgggg agacggagaa gcagctgcac
     3361 acgggccccc aggccgcctc agttctgggc atcccgaccg tgcccacgac gcgcttcgag
     3421 accgcctgcg cgtcctcgca cgcggccttc cggcacgcgg tgatggagat cgccgccggc
     3481 gtgtccgacg tggtcctggt gggcggcgcc gaacgggtgc tcaacgtgcc caccgccgat
     3541 tcgaccgagt acttcgcgta cgcctccgac gcctcgtggg agcagccggc cggcctcacc
     3601 tttcccggcg tcttcgccct cgtcgcccgc gcccacatga cgaagcacgg gaccaccgag
     3661 gagcagatgg cccacgtggc cgtgaagaac caccgccacg gggccctgaa cccgaaggcc
     3721 cagttccaga aggagatcac gctccagcag gtgctcgcgt ccgcgtacgt cgccgacccg
     3781 ctcaagctct acgattgctg cccgttcacg gacggcggcg ccgcggtggt cctggcgtcc
     3841 gaggaggtcg cgcgcacgcg ccgacgcgcg gtctgggtgc tgggctcggc ggcagcgtcc
     3901 gacgcgatgc tgctccacga caagcgagat ctggcccgca tccccgccac cgagcgcgcg
     3961 gcagccgccg cctaccgcca ggcgggcctc gggccccgcg acgtgaacgt ggtcgagctg
     4021 cacgactgct tcacgatcgc cgagatcgtc gcgacggagg gtctcggcct cttcgagccg
     4081 ggcacgggcg gcgcggcggc cgagaagggg tggacgagcc tcggcggcaa gatccccgtg
     4141 aacacctcgg gcgggctcaa agccaagggc catcccatcg gggccaccgg cgccgcccag
     4201 gtcgtggagg tggtgaccca gctccgcggc gaagccggtg cgcgtcaggt ggacggcgcc
     4261 cgcatcgggc tcacccatac cctcggcggc aacaccgcga ccgtgctcgt gagcctcttc
     4321 ggccgtgact gagccggtga tcacgctcaa gcacttcttc gagcgggtcc gggacggcgc
     4381 gctgacggcc atcaagtgcg gcgcgtgcgg tgagctggcg atcccgccca aggagttctg
     4441 tgccgggtgc ggcca
//
LOCUS       DATIAZ010000300         4071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_742557, whole genome shotgun sequence.
ACCESSION   DATIAZ010000300 DATIAZ010000000
VERSION     DATIAZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742557"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..276
                     /gene="iscX"
                     /locus_tag="VLF19_10425"
     CDS             76..276
                     /gene="iscX"
                     /locus_tag="VLF19_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HSE93711.1"
                     /translation="MTWRDAEDIGIALAGARPDTDPLTVRFTDLHRWVTELPGFADDP
                     KASSESMLEKIQMAWLEEYRNR"
     gene            complement(321..545)
                     /locus_tag="VLF19_10430"
     CDS             complement(321..545)
                     /locus_tag="VLF19_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93712.1"
                     /translation="MAAVLHLLKRSDADLALATLAGQLAAGDRVAVALLHGAPRPKLP
                     EAVQVVRVPEDTSYEKLLELIFESDHVTAW"
     gene            complement(545..904)
                     /locus_tag="VLF19_10435"
     CDS             complement(545..904)
                     /locus_tag="VLF19_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSE93713.1"
                     /translation="MDTVEPVLVVISTDPRSSHRAFEALRIGVGIVAGENEVTFVLAD
                     GAVHLLDDDTDALVDGDDIAKFRATLRTMDVPFHVEASAVPPSDAEWNVEHHPVVTVS
                     RDEIAALARRARRALVF"
     gene            complement(942..1265)
                     /locus_tag="VLF19_10440"
     CDS             complement(942..1265)
                     /locus_tag="VLF19_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSE93714.1"
                     /translation="MDLARKKLGVLLSTGPEHDNLATAIGLGTAALHRGADLYLYLID
                     DGVRALGDPGLRALSARGAKLFVCAYGCQKRRIPLEDAADVTYCGLVVLTDIINATDR
                     FVALN"
     gene            1307..2464
                     /locus_tag="VLF19_10445"
     CDS             1307..2464
                     /locus_tag="VLF19_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSE93715.1"
                     /translation="MSRRVYMDHNASTPVHPEVVAEMAPYFADVFGNPSSIHAFGRAA
                     RDAVDAARDRVARFLGVAADEIVFTSGGTESDNLAVKGLARARGGGHLITSRIEHHAV
                     LRTCQALETQGFAVSYVPVDGHGRIDPGDVARAIRPDTIAISVMHANSEVGTIEPIAE
                     IGAVAREQGVPLHVDAVQTFGKIPVDVRALGVDVLSFSSHKIYGPKGVGGLWIRKGTR
                     MAADQHGGDHERRRRAGTENVPGIVGFGKAVEIRGRDMAAEAERLRGLRDRLWDGARA
                     RIPEVRLNGHPTERLPGTDNLAFRNVESESVVLGLDLKGVGVSAGSACTSGHVEPSHV
                     LVAMGVPLDWAMGAVRFSLGGSTTADDVAYVVECLVSIVAKLRRALPVGAA"
     gene            2461..2841
                     /locus_tag="VLF19_10450"
     CDS             2461..2841
                     /locus_tag="VLF19_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HSE93716.1"
                     /translation="MKYSATLLDHFMNPRNAGLMRDPDGVGQDEYAGCGDLARFFLRV
                     RDGRAAELRFQTYGCGPTIAAASAASERLAGRAVEELADVRAEEIERALDGLPDDRKH
                     AADVVAGAVRAAARDHLRRGGRDV"
     gene            2834..3496
                     /gene="cysE"
                     /locus_tag="VLF19_10455"
     CDS             2834..3496
                     /gene="cysE"
                     /locus_tag="VLF19_10455"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HSE93717.1"
                     /translation="MFETIRRDVRAVLERDPAARSTVEVLLCYPGVHALAFHRLAHRL
                     WTAGWRTNARFLSHVARFLTGIEIHPAARLGPGLFIDHGMGVVIGETAEVGENVTLLQ
                     GVTLGGTSLRREKRHPTLGDNVVVGAGAKVIGAFKIGAGSRIGAGSVVVREVPPNSVV
                     VGVPGRVTYRDGQRVEAIDLNQADLPDPVARMVEQLVERVRELEGEIEALRKQVDADR
                     LK"
     gene            3510..3692
                     /locus_tag="VLF19_10460"
     CDS             3510..3692
                     /locus_tag="VLF19_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSE93718.1"
                     /translation="MTPKDVMSMQEACTYLAMDEPTLTRLAAERRIPCFQENGAWVFS
                     KKSIDKWRSLKERARA"
     gene            3697..>4071
                     /locus_tag="VLF19_10465"
     CDS             3697..>4071
                     /locus_tag="VLF19_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSE93719.1"
                     /translation="MLDRGAAWSILTEFTKSDSLRKHARAVEASMRAYAIRYGEDPEA
                     WGVAGMLHDFDYEMHPAAPRHPMKGAEILTARGVAPPVIYAILAHADYSGMPRVSRLD
                     RALYACDELSGFVHACALVRPGK"
BASE COUNT          596 a         1379 c         1520 g          576 t
ORIGIN      
        1 gctgacgatt cactcgcggc tcgggtgcca ggcggtggtg cgcggtgatg tcgtggtgga
       61 ggtgcccaag tgaagctgac gtggcgagac gccgaggaca tcgggatcgc gctcgcgggg
      121 gcgcgcccgg acacggatcc cctcacggtc cggttcacgg acctgcaccg ctgggtgact
      181 gaactcccgg ggttcgccga cgaccccaag gcctccagcg agagcatgct cgagaagatc
      241 cagatggcgt ggctcgagga gtaccggaac cgatgagccg gctccatcgg cgctcgtggc
      301 cgtcgctccc gccggcgctt ctaccaggcg gtcacgtgat cggactcgaa gatcagctcg
      361 aggagcttct cgtaggaggt gtcttcgggc acgcgcacga cctggacggc ttcggggagc
      421 ttcggccgag gcgcgccatg gaggagggcg acggcgacgc ggtcgccggc ggcgagctgg
      481 ccggcgaggg tcgccagcgc gaggtcggcg tcactgcgct tgagcaggtg gaggacggcg
      541 gccatcagaa gacgagggcg cggcgggcgc ggcgggcgag cgcggcgatc tcgtcgcggc
      601 tcaccgtgac cacgggatgg tgctcgacgt tccactcggc gtcgcttggc ggcaccgcgg
      661 aggcttcgac gtggaagggc acgtccatcg tccgcagcgt ggcccggaac ttggcgatgt
      721 cgtcgccgtc gaccagcgcg tcggtgtcgt cgtcgagcag gtggacggcg ccatcggcga
      781 gcacgaaggt gacctcgttc tcaccggcga cgatgccgac gccgatccgg agcgcctcga
      841 aggcgcggtg gctcgagcgc gggtcggtcg agatcacgac gaggacgggc tcgacggtgt
      901 ccatcgacgc ggctccgtcg gacatcccgc cgtcgggcat ctcagttgag cgcgacgaaa
      961 cggtcggtgg cgttgatgat gtccgtgagc accaccaggc cgcagtaggt gacgtcggcc
     1021 gcgtcctcga gcgggatgcg gcgcttctgg cagccatacg cgcagacgaa gagcttcgcg
     1081 ccgcgcgccg agagcgcgcg cagacccggg tcgcccaggg cccgcacgcc gtcgtcgatg
     1141 agatagagat acaggtccgc gccgcggtgg agcgccgccg tgccgaggcc gatcgcggtg
     1201 gcgagattgt cgtgctcggg cccggtggac agcagcacgc cgagcttctt gcgcgcgaga
     1261 tccatcgcgc accgagccta acattcgctt caggagtgag caacttatga gccgtcgcgt
     1321 ctacatggac cacaatgctt ccacgccggt gcatcccgag gtcgtcgcgg agatggcccc
     1381 gtatttcgcc gacgtcttcg gcaacccctc gagcatccac gcgttcggcc gcgccgcgcg
     1441 tgacgccgtc gacgccgccc gtgaccgcgt ggcacgcttc ctcggcgtcg ccgccgacga
     1501 gatcgtgttc acctccggcg gcacggagtc cgacaacctc gcggtcaagg gactcgcgcg
     1561 ggcgcggggt ggtggtcacc tgatcacgtc gcggatcgag caccacgcgg tgctgcggac
     1621 gtgccaggcg ctggagaccc agggcttcgc cgtcagctac gtgccggtgg acgggcacgg
     1681 ccggatcgat cccggcgacg tggcgcgggc catccgcccc gacacgatcg ccatcagcgt
     1741 catgcacgcc aacagcgagg tcggcacgat cgagcccatc gcggagatcg gggcagtggc
     1801 ccgcgagcag ggcgtgccgc tgcacgtgga cgcggtgcag acgttcggca agattcccgt
     1861 ggacgtgcgc gcgctcgggg tcgacgtgct gtcgttctcg agccacaaga tctacgggcc
     1921 gaagggagtg ggggggctct ggatcaggaa ggggacgcgg atggccgccg atcagcacgg
     1981 cggcgatcac gagcggcgcc gccgggccgg caccgagaac gtgccgggca tcgtgggctt
     2041 cggcaaggcc gtcgagatcc gcggccggga catggccgcc gaggcggagc ggcttcgggg
     2101 gctgcgtgac cgcctctggg acggcgcacg ggcgcggatc cccgaggtgc ggctcaacgg
     2161 gcacccgacc gagcgcctgc ccgggaccga caacctggcg ttccggaacg tcgagtccga
     2221 gtcggtcgtg ctcggcctcg acctcaaggg ggtcggcgtc tcggccgggt cggcgtgcac
     2281 gtcgggccac gtggagccat cgcacgtgct ggtggccatg ggcgtgccgc tcgactgggc
     2341 gatgggcgcc gtgcggttct ccctcggcgg ctcgacgacg gccgatgacg tggcctacgt
     2401 ggtcgagtgc ctcgtgtcca tcgtcgccaa gctgcggcgc gcgttgccgg tcggcgccgc
     2461 gtgaaataca gcgccacgct gctcgaccac ttcatgaacc cgcgcaacgc cgggctcatg
     2521 cgggatcccg atggcgtcgg gcaggacgag tacgccggct gcggcgacct cgcgcgcttc
     2581 ttcctccgcg tgcgcgatgg ccgcgcggcc gagctgcggt tccagacgta cggctgcggg
     2641 ccgaccatcg ccgctgccag cgcggccagc gagcggctgg ccgggcgcgc ggtcgaggag
     2701 ctcgccgacg tgagggcgga ggagatcgag cgcgcgctcg acggcttgcc cgacgaccgc
     2761 aagcacgccg ccgacgtggt ggccggcgcc gtgcgggccg ccgcgcgcga tcacctgcgc
     2821 cggggaggac gggatgtttg agacgatccg ccgtgacgtg cgcgccgtgc tcgagcgtga
     2881 ccccgcggcg cgctcgaccg tcgaggtgct cctctgctac ccgggggtgc acgccctcgc
     2941 cttccaccgc ctggcgcatc gcctgtggac cgccggctgg cgtacgaacg cgcgattcct
     3001 ctcccacgtc gcccggttct tgacgggcat cgagatccac ccggcggcgc ggctcgggcc
     3061 cgggctcttc atcgatcacg gcatgggggt ggtcatcggc gagacggccg aggtcggcga
     3121 gaacgtgacg ctgctgcagg gcgtgacgct gggcggcacc agcctcaggc gcgagaagcg
     3181 gcatccgacg ctcggcgaca acgtcgtggt cggcgccggt gccaaggtca tcggcgcctt
     3241 caagatcggc gcgggcagcc gtatcggtgc cggctcggtc gtcgtgcgcg aggtgccgcc
     3301 gaactccgtt gtggtcggcg tccccggccg cgtaacatac agggatggtc agcgggtcga
     3361 ggcgatcgac ctgaaccagg cagacctgcc cgatccggtg gcgaggatgg tcgagcagct
     3421 cgtggagcgg gtgcgcgagc tggagggcga gatcgaggcg ctgcgcaagc aggtcgacgc
     3481 cgacaggctc aagtgaaggg ggacacggcg tgactccgaa ggacgtgatg agcatgcaag
     3541 aggcgtgcac ctacctcgcc atggacgagc cgacgctgac gcgcctggcc gccgagcgcc
     3601 gcatcccgtg cttccaggag aacggggcgt gggtcttctc caagaagtcc atcgacaaat
     3661 ggcgcagcct caaggagcgc gcccgcgcct gagccgatgc tcgatcgcgg cgccgcctgg
     3721 tcgatcctca ccgagttcac caagagcgac agcctccgca agcacgcccg cgccgtcgag
     3781 gcgtccatgc gcgcctacgc cattcgctac ggcgaggacc ccgaggcctg gggcgttgcc
     3841 ggcatgcttc acgacttcga ctacgagatg cacccggcgg cgccgcgtca cccgatgaag
     3901 ggagccgaga tcctgacagc gcgcggcgtc gccccgcccg tgatctacgc gatcctcgcc
     3961 cacgccgact actccggcat gccgcgcgtc tcgcggctcg accgggcgct ctacgcctgc
     4021 gacgagctct ccggcttcgt ccacgcctgc gcgctcgtgc ggccgggcaa g
//
LOCUS       DATIAZ010000301         5739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1664821, whole genome shotgun sequence.
ACCESSION   DATIAZ010000301 DATIAZ010000000
VERSION     DATIAZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664821"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VLF19_10470"
     CDS             complement(<1..122)
                     /locus_tag="VLF19_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSE93720.1"
                     /translation="METADVVVIGGGANGASTAFSLASLGVRRVILLERSALGAG"
     gene            complement(226..393)
                     /locus_tag="VLF19_10475"
     CDS             complement(226..393)
                     /locus_tag="VLF19_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93721.1"
                     /translation="MRPALANHDQGSGQSQDHLHRRNPGAVNVVTTAEPLAPAAVGIG
                     LLTLGFLRRRQ"
     gene            complement(432..1139)
                     /locus_tag="VLF19_10480"
     CDS             complement(432..1139)
                     /locus_tag="VLF19_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSE93722.1"
                     /translation="MAAVLGGWEAAARAGLVDPLFVPAPSAVGAALVAMGGSALGALG
                     ETLAKTAVAYVIAAVLGVTGGIALGAVPPLRETLHPFVVALYGMPKILVLPWIVLLLG
                     FGVVPAVVYGVVHGLFPILLLVLGAVRDVDRTLLTVARAYGARPWQVYWKVVLPAIVP
                     AVLASLRLGIVFCLLGVLIVEMFAGVRGMGHVLNSLANGFQAARLFAATTLVSAASIV
                     IVLALDHVSETLSRWRG"
     gene            complement(1247..1993)
                     /locus_tag="VLF19_10485"
     CDS             complement(1247..1993)
                     /locus_tag="VLF19_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSE93723.1"
                     /translation="MRIQAARALVLLVGLGAWEAVARLGNPYLYVPPSAVLPALAGVV
                     TLRTYPDLPEHLLLTAREIAAAYGLAVAAGLGVGGVLGASRVLGRAYGPILAALYAVP
                     AVVWYPSLMLFFGLDAASKIAFGFLLGFFPITLAVLAGIRQVSPQLVTVARSFGASPL
                     TVFVKVLAPGMLFTLAGGLRTGLALSVVGVVVGEVLGAKAGMGALINHAYGLARTADY
                     VAVVVVTLALIVASDALASLVERRARRWTT"
     gene            complement(2034..2810)
                     /locus_tag="VLF19_10490"
     CDS             complement(2034..2810)
                     /locus_tag="VLF19_10490"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93724.1"
                     /translation="MPPLIAIEGVAKRYETSSGAVDALRDVSLAVGEGEFCTIIGPSG
                     CGKSTLLGMVGGLVSPDAGRVLVEGRPVAGPDPRRMATVFQDPGLFPWRTTLENIEFG
                     LEMQGVDRARRRQAATQLLGPLGLKGFAEKYPRELSGGMRQRAAIGRALAIDTKMLLM
                     DEPFGALDEQTRVLMGEWLLDIWRRTGKTVLFVTHSLHEALALSTRVAVMTARPGRIK
                     SVIELALPYPRAMESPELSTLRAKLWSEIRDESLRSMSES"
     gene            complement(2829..3974)
                     /locus_tag="VLF19_10495"
     CDS             complement(2829..3974)
                     /locus_tag="VLF19_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE93725.1"
                     /translation="MDFELTPEQRAVRDAAREFAEAELGEKIAPYDERHEFPWPVVAR
                     LGEVGFLGALAPQEYGGAGLDYVSYALLVEELNRGDASVGITMWAHNSLCTNHILTFG
                     SPAQKAKYLPPLARGEVLGAWGLTEPGSGSDAAALRTRAEPREGAWVLNGSKAFITNA
                     SVGTTAVVMARTDPEKKSRGISAFVLEKGFPGFSAAKPYRKLGLHASDTAELIFEDAR
                     VPAENLLGERGQGFVQAMQVLEGGRIAMAAMGVGIAQAALDQAVKYMKQRTAFGQPLA
                     DFNGLQGMVADLATEIETARLLTLQAAWLKDQGRSAMHAAAMAKLFASETAMKAATKA
                     VQIHGGAGYVTEFPVERIFRDAKLTEIGEGTSEVQRLVIAREILALR"
     gene            complement(3980..4144)
                     /locus_tag="VLF19_10500"
     CDS             complement(3980..4144)
                     /locus_tag="VLF19_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93726.1"
                     /translation="MIRKIGRKYVVLSEKTGRRFGTYDTQAEAERRLRQVEFFKHARG
                     GTRRRTRGAA"
     gene            complement(4172..4426)
                     /locus_tag="VLF19_10505"
     CDS             complement(4172..4426)
                     /locus_tag="VLF19_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93727.1"
                     /translation="MPPSDDELKAEVERLRAENAALKARAPGSRNLKVSEKGAVSLYG
                     LGRFPVTLYKEQWVRLLDMAEEIRTFIREHESELKAKEPR"
     gene            complement(4413..4817)
                     /locus_tag="VLF19_10510"
     CDS             complement(4413..4817)
                     /locus_tag="VLF19_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSE93728.1"
                     /translation="MSERTIRVVVAKPGLDGHDRGAKIVARALRDAGFEVIYTGLHQT
                     PEQVVATAIQEDADAIGLSVLSGAHNYLFTRLMELLREKGAEDVVVFGGGIIPPEDVA
                     ALKALGVKELFGPGTSTQDIVRFVREHVRAAV"
     gene            complement(4814..5239)
                     /locus_tag="VLF19_10515"
     CDS             complement(4814..5239)
                     /locus_tag="VLF19_10515"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSE93729.1"
                     /translation="MKDCVFCKIRDGEVPSLKVYEDERTLCFMDINPLTSGHCLVMTR
                     AHAPTLFEADVDDLKAAIATAKTVALALLRAVEPDGLNMLQANGAAAFQSVPHYHLHL
                     IPRWNGDGKGFDWKLVPGDRAQITKVGERLRAVLSGGPS"
     gene            complement(5257..>5739)
                     /locus_tag="VLF19_10520"
     CDS             complement(5257..>5739)
                     /locus_tag="VLF19_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSE93730.1"
                     /translation="KTAANYAASLYAAEEAKHEGFTQVLWLDAVHRQYIEEVGTMNIM
                     LKIADEVITPPLAGTILAGVTRDSVLTLLREWGHRVSERPITIDEVREAAHRGTLREV
                     FGTGTAAVISPVGELAYKDERIVVNDGRIGELTQRLYDTIVGIQYGTARDAHGWTVVV
                     "
BASE COUNT          843 a         2087 c         2035 g          774 t
ORIGIN      
        1 ccggcgccga gcgcactgcg ctcgagcagg atgacgcggc ggacgccgag gctggcgagg
       61 ctgaaggcgg tgctggcccc gttggcacct ccgccgatca cgacgacgtc ggccgtctcc
      121 acaccgcgat tgtgacacag cgagccgatt cgccgctcgc ccggcggcgc gcgatcgtcg
      181 gcgcatcgcg gcggaggtgc tccgcagggc ccggggaggg accggctact gccggcgccg
      241 caggaacccc agggtgagga gcccgatccc gaccgcggcc ggcgccagcg gctcggcggt
      301 ggtgacgacg ttcaccgcgc cggggttccg acggtgcaga tggtcctgcg actggccact
      361 gccctggtcg tggttcgcga gcgccggcct cacgagggcg agcgccagag gaagttggca
      421 gatcctcgaa cctatccccg ccagcgcgag agggtctcgc tgacgtggtc gagagcgagc
      481 acgatgacga tggaggcggc ggagacgagc gtggtcgcgg cgaagagacg cgcggcctga
      541 aagccgttgg cgagcgagtt gagcacgtgg cccatgccgc gcacgccggc gaacatctcg
      601 acgatcagca cgcccagcag gcagaagacg atgccgagcc ggaggctggc aagcacggcg
      661 ggcacgatcg cgggcaggac gaccttccag tagacctgcc agggccgcgc gccgtaggcg
      721 cgggccaccg tcagcagcgt gcggtcgacg tcgcggacgg cgccgagcac cagcaggagg
      781 atcgggaaca ggccgtggac gacgccgtaa accacggccg gcacgacgcc gaagccgagc
      841 agcaggacga tccacgggag cacgaggatc ttcggcatgc cgtagagggc caccacgaac
      901 gggtgcagcg tctcccgcag cggcggcacg gcgcccaggg cgatgccgcc ggttaccccg
      961 agcacggcag cgatgacgta ggcgacggcg gtcttggcga gcgtctcgcc cagcgcgccc
     1021 agcgccgagc cgcccatggc gaccagcgcg gcgccgactg ccgacggcgc cggcacgaac
     1081 agcggatcga cgaggccggc gcgggccgcg gcctcccagc ccccgaggac agcggccacg
     1141 ccaaacgctt gcagcagccg gacgcgcgcc gtctcgcgac cggagcgagg tccacgcggg
     1201 cggctcgcgg catgggcggc cggaccctcc gaggcccggc ggcgtttcac gtcgtccacc
     1261 gccgcgcccg gcgctcgacc aggctcgcca gcgcgtcgga ggccacgatg agcgccagcg
     1321 tcaccacgac caccgccacg tagtccgcgg tccgcgcgag cccgtaggca tggttgatca
     1381 gcgcgcccat gccggccttc gcgccgagca cctcgccgac gaccaccccg acaacggaca
     1441 gcgccaggcc cgtgcggagc ccgccggcca gggtgaacag catgcccggc gccagcacct
     1501 tgacgaagac ggtgagcggc gaggcgccga agctccgggc caccgtgacc agctgcgggc
     1561 tcacctggcg gatgccggcc aggaccgcga gcgtgatcgg gaagaagccg agcaggaagc
     1621 cgaacgcgat cttggacgcc gcgtcgagcc cgaagaacag catcagcgac gggtaccaga
     1681 cgacggctgg caccgcatag agcgcggcga ggatcgggcc gtaggcgcgg cccagcacgc
     1741 ggcttgcgcc gaggaccccg ccgacgccga gcccggcggc caccgcgagg ccgtaggccg
     1801 cggcgatctc gcgcgcggtc agcagcaggt gctccggcag gtcgggatac gtgcggagcg
     1861 tcaccacgcc ggcgagggcc ggcagcacgg ccgacggcgg cacgtagagg taggggttgc
     1921 cgagccgcgc gacggcctcc caggcaccga gaccgacgag caacacgagc gcgcgcgccg
     1981 cctggatcct cacgtccggt ctccgcgcgt cgccccggtg cgagcgacgc cgctcacgac
     2041 tcgctcatgg accgcagcga ctcgtcgcgg atctcgctcc agagcttggc gcgcagcgtg
     2101 gagagctccg gcgactccat cgcgcgcgga tacggcagcg ccagctcgat gacgctcttg
     2161 atccgccccg ggcgcgcggt catgacggcc acccgcgtcg agagcgcgag cgcttcgtgc
     2221 aggctgtgag tcacgaagag cacggtcttg cccgtccggc gccagatgtc gaggagccac
     2281 tcgcccatca gcacgcgcgt ctgctcgtcg agcgcgccga acggctcgtc catgaggagc
     2341 atcttcgtgt cgatggccag cgcgcgtccg atggccgcgc gctggcgcat cccgcccgac
     2401 agctcccgcg ggtacttctc ggcgaagccc ttgagcccga gcgggcccag cagctgcgtg
     2461 gccgcctgcc gccgccgggc ccgatccacg ccctgcatct cgagcccgaa ctcgatgttc
     2521 tccagcgtgg tccgccacgg gaagaggccc gggtcctgga acaccgtggc catccgtcgc
     2581 gggtcggggc cggcgacggg ccggccctcg acaagcacgc gaccggcgtc gggactgacg
     2641 aggccgccca ccatgcccag gagcgtggac ttgccgcacc ccgaaggccc gatgatcgtg
     2701 cagaactcgc cctcgccgac ggcgagcgac acgtcccgga gggcgtcgac ggcgccggag
     2761 gacgtctcgt agcgcttggc gacgccctcg atcgcgatga gggggggcac gcgccgggat
     2821 ccggcccgtc agcgcagggc gaggatctcg cgggcgatca cgagccgctg gacctccgag
     2881 gtgccctcgc cgatctcggt gagcttagcg tcgcggaaga tgcgctcgac cgggaactcg
     2941 gtgacgtagc cggcgccgcc gtggatctgc accgccttgg tcgccgcctt catcgccgtc
     3001 tccgaggcga agagcttggc catcgccgcg gcgtgcatcg ccgaccggcc ctggtccttg
     3061 agccaggcgg cctgcagcgt gagcagccgc gccgtctcga tctcggtggc gaggtcggcc
     3121 accatcccct gcaggccgtt gaagtcggcg aggggctggc cgaacgccgt gcgctgcttc
     3181 atgtacttga cggcctggtc gagcgcggcc tgggcgatgc ccacgcccat cgccgccatc
     3241 gcgatgcggc cgccctcgag cacctgcatg gcctgcacga agccctggcc gcgctcgccc
     3301 aggaggttct cggccggcac gcgcgcgtcc tcgaagatca gctcggcggt gtccgaggcg
     3361 tgcaggccga gcttgcggta gggcttcgcc gccgagaacc cggggaagcc cttctcgagc
     3421 acgaacgccg agatgcccct cgatttcttc tcgggatccg tgcgcgccat caccaccgcc
     3481 gtcgtgccga cgctggcgtt ggtgatgaag gccttgctgc cgttgagcac ccaggcgccc
     3541 tcgcgcggct cggcgcgcgt gcggagcgcg gcggcgtcgg agcccgagcc cggctcggtc
     3601 agcccccacg cgcccagcac ctcgccccga gccagcggcg gcaagtactt cgccttctgg
     3661 gccggcgagc cgaacgtgag gatgtggttc gtgcagagcg aattgtgggc ccacatcgtg
     3721 atgccgaccg aggcgtcgcc gcggttcagc tcctcgacga gcagcgcgta cgagacgtag
     3781 tcgagcccgg ccccgccgta ctcctggggc gcgagcgcgc cgaggaagcc cacctcgccg
     3841 agcctggcca cgaccggcca cgggaactcg tggcgctcgt cgtagggcgc gatcttctcg
     3901 cccagctcgg cctcggcgaa ctcccgggcg gcgtcgcgca ccgcccgctg ctcgggcgtg
     3961 agctcgaagt ccatggtcct caggccgcgc cgcgcgtgcg cctgcgcgtc ccgcccctgg
     4021 cgtgcttgaa gaactcgacc tgtcgcaggc gccgctcggc ctcggcctgc gtgtcgtacg
     4081 tcccgaagcg gcggccggtc ttctccgaga ggaccacgta cttgcggccg atcttcctga
     4141 tcacgggatc actccccgcc tgcccgatgc gtcaccgcgg ctccttcgcc ttcagctcgg
     4201 actcgtgctc tcggatgaag gtgcggatct cctcggccat gtcgagcagg cgcacccact
     4261 gttccttgta gagcgtcacc gggaagcggc cgagcccgta caggctgacc gcgcccttct
     4321 cgctgacctt gaggttccgg gagcccggag cccgtgcctt gagcgctgcg ttctcggcgc
     4381 ggagtcgctc gacctccgcc ttcagctcgt cgtcagacgg cggcacggac gtgctcccgg
     4441 acgaaccgga cgatgtcctg ggtggaggtg cccggcccga acagctcctt gacgccgagc
     4501 gccttgagcg ccgccacgtc ctcggggggg atgatccccc cgccgaacac gacgacgtcc
     4561 tcggcgccct tctcgcggag cagctccatc agccgcgtga agaggtagtt gtgcgcgccc
     4621 gacaggacgc tgaggccgat ggcgtcggcg tcctcctgga tcgccgtcgc tacgacctgt
     4681 tcgggcgtct ggtgcaatcc cgtgtagatg acctcgaagc ccgcgtcccg gagcgcgcgt
     4741 gcgacgatct tcgcccctcg gtcgtggccg tccagccccg gcttcgccac cacgactcgg
     4801 atcgtccgct cgctcatgaa gggccaccgg agagcaccgc ccgcaggcgc tcgccgacct
     4861 tcgtgatctg ggcgcggtcg cccggcacga gcttccagtc gaagcccttc ccgtcgccgt
     4921 tccagcgtgg aatgaggtgc aggtgatagt gcggcaccga ctggaaggcg gcggcgccgt
     4981 tggcctgcag catgttgagc ccgtccggct cgaccgcccg gagcagggcg agcgcgacgg
     5041 tcttggccgt cgcgatggcc gccttcaggt cgtccacgtc ggcctcgaag agggtcggcg
     5101 cgtgggccct ggtcatcacc aggcagtggc ccgaggtgag cgggttgatg tccatgaagc
     5161 agagcgtccg ctcgtcctcg tacaccttca acgaagggac ctcgccgtcc cggatcttgc
     5221 agaacacgca gtccttcatc gagcccggcc tcccagctag acgaccacgg tccagccgtg
     5281 cgcgtcgcgc gccgtcccgt actggatgcc gacgatggtg tcgtagagcc tctgcgtcag
     5341 ctctccgatc cgcccgtcgt tgaccacgat gcgctcgtcc ttgtaggcga gctcgcccac
     5401 cggcgagatg acggcggcgg tgcccgtgcc gaacacctcg cgcagcgtgc cgcggtgggc
     5461 cgcctcccgc acctcgtcga tggtgatcgg tcgctcggaa acgcgatggc cccactcgcg
     5521 cagcagcgtg aggaccgagt cgcgggtgac gccggcgagg atggtcccgg cgagcggcgg
     5581 cgtgatcacc tcgtcggcga tcttcagcat gatgttcatg gtgccgactt cctcgatgta
     5641 ctggcgatgg accgcgtcga gccagagcac ctgcgtgaag ccctcgtgct tcgcctcctc
     5701 ggcggcatag aggctggccg cgtagttcgc cgcggtctt
//
LOCUS       DATIAZ010000302         6355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1268561, whole genome shotgun sequence.
ACCESSION   DATIAZ010000302 DATIAZ010000000
VERSION     DATIAZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268561"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..866)
                     /locus_tag="VLF19_10525"
     CDS             complement(129..866)
                     /locus_tag="VLF19_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSE93731.1"
                     /translation="MTTLQRAEAAEHAMSQELDRVVVKSVIYTSGERDPRQPIPRPPD
                     QGKRYMMGHDPRLSRMPDQPTLFDFFRHRFGPTAHLLQSARLALKNGTSEKVVLACLL
                     HDIAIAGFIRGDHGYWGAQLLEPYVDEEVSWAIRYHQALRFFPDESVGYRYPELYLKL
                     FGADYMPEPYIHEAYRYARNHKWYMTSRLITVNDLYAFDPGVQVELEEFTDVVGRNFK
                     QPKEGLGLDASPSAHMWRTIMWPTRYL"
     gene            complement(994..2181)
                     /locus_tag="VLF19_10530"
     CDS             complement(994..2181)
                     /locus_tag="VLF19_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HSE93732.1"
                     /translation="MIGFNPMDPEFLADPYPTYHRLRAEDPVHHSPLGFWVLTRYEDV
                     VAVLRDPRCVKEPIAALIAARFGVAVPPGIGLSMLDRDPPDHTRLRSLVSKAFTPRVV
                     QRLRLHVQQIVDGLLDRAEAAGSMDLIEQFAYPLPVIVICEMLGVPVEDHERFKGWGL
                     DIARGLDSILLPPDSEVPRRSAAARHALSDYFRSLIVERRAAPRDDLLSALIAAEEAG
                     DTLSEDELLATCILLLVAGHETTVNLIGNGTLALLRHPGELQRLRESPSLLASAVEEL
                     LRFDGPVQRTARVPSEDVTIGGRTIGKGEMVMPFIGAADRDPAQFPEPDRLDIARADN
                     RHIAFGWGIHFCLGAPLARLEGQIAIGTLVRRLPKLALAVDRPEYRQSLTLRGLQALP
                     VSF"
     gene            complement(2178..3119)
                     /locus_tag="VLF19_10535"
     CDS             complement(2178..3119)
                     /locus_tag="VLF19_10535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE93733.1"
                     /translation="MAVEFGVLLPTREAIMSGRPDTAPLLAMAERAEAAGFDSVWIGD
                     SLTARPRHEPLTLLAAVAARTRRVRLGTGVLLPVLRNPVVLAHVVGTLDRIAEGRVIL
                     GVGIAADTPAIRKEFAEAGVPFERRVGRFLETLEICRALWGRDDVSFNGKHFTLAHVT
                     LEPKPHRPGGPPIWIGGSGPTALREAARFDAWFPTGPSVEFFAEHFPRVRAAARAAAR
                     ASEDVTGAAYVTLALDASRAAAEQRLHAFLETYYAAPARAILARQATYAGPVEGCVEW
                     LQRWIDAGARHISLRFAGGDQLAQVDEAAARLLPRLR"
     gene            complement(3270..4016)
                     /locus_tag="VLF19_10540"
     CDS             complement(3270..4016)
                     /locus_tag="VLF19_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSE93734.1"
                     /translation="MPPDAARKLEVVTDVPDRAMVVFAHPDDAEIGSGGVVAKWVAAG
                     CEVTYVLCTNGHAGTADRSLSPEALAKKRADEQRAAADAMGVKHVVMLGYPDGELEDT
                     REVLGDIVRALRRYRPHTVFVHDPYRIKGFQHRDHRKAGLVTTDAVYPYARDHLHFPE
                     HIAKDGLEPHRVRELWYWGMDEPDVIVDVSDSIERQIAALIRHESQVPGFNVPAGQTI
                     GERVKKNAAAHAAGYGFEYGAVFRRLIARR"
     gene            complement(4070..4723)
                     /locus_tag="VLF19_10545"
     CDS             complement(4070..4723)
                     /locus_tag="VLF19_10545"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSE93735.1"
                     /translation="MAVRIGDEAPDFTAETTEGKIRFHEWIGDRWAILFSHPKDFTPV
                     CTTELGYMAGLKPEFDKRNTKIIGLSVDPVGDHKAWVKDIQETQGHAVNYPLIGDADL
                     TVAKLYDMIHPNAAGGQRTAIDNATIRSVFIIGPDKKVKAMLVYPMSAGRNFDEVLRL
                     LDSLQLNAKHAVATPVNWKPGQDVIIPTNVSDEEAKKKYPQGFKTHKPYLRTVAQPK"
     gene            4867..6033
                     /locus_tag="VLF19_10550"
     CDS             4867..6033
                     /locus_tag="VLF19_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011428687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSE93736.1"
                     /translation="MKISEVQATWVHVPIPHERQHVSDFGRVASFDSVVVKVVTDGGL
                     VGWGEAKAGVGSAASAHGLAAIIGRDYAPLLVGQDPRDVSRLWDVMYNTPREGYAVDR
                     GHVLPQLGRRGLSIPAIAGVDIALWDLLGKSLGAPVWRLLGGRRAERMPAYASGGWAD
                     EARIGEQLLGYVEKGGFRAVKMRVGVMDGEPHRSAARVRAARAAIGPDIKLMADAHGT
                     WTVSEAKAFCRMVEDCDLYWLEEPVTADDKTGLAEVRRASAVPIATGESEFTRHDFRE
                     IAELRAADILQPDLAIAGGITEGVRIGAIASAYNLRLAPHLWSGAPAFAAGVALAASQ
                     SAGFILEYSLGANPLLHELVHEQFPVMDGHVEIPDRPGLGITVDEDFVRRHARH"
     gene            6105..>6355
                     /locus_tag="VLF19_10555"
     CDS             6105..>6355
                     /locus_tag="VLF19_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSE93737.1"
                     /translation="MKVKAVNGFARILKAEGIPWVSCYPTSPVNNALGEEGVPILMMG
                     EERFAVAVADAFSRVTSGKQIGACTVMAGLNAAGIQMAYG"
BASE COUNT          915 a         2144 c         2330 g          966 t
ORIGIN      
        1 catcttcggg atggggcgag gttccgtttg gcggggaaca ggtcgacgga cccgcgcccc
       61 gaccccaccc cgaccggcgc attcgtcggc gagcttccga tgtcggcgag cttctgaccc
      121 gggggagact acaggtagcg cgtcggccac atgatggtgc gccacatgtg cgccgacggg
      181 ctggcgtcga gacccagccc ctccttcggc tgcttgaagt tccgcccgac cacgtcggtg
      241 aactcctcga gctcgacctg cacgccgggg tcgaaggcgt agaggtcgtt cacggtgatc
      301 aggcgcgagg tcatgtacca cttgtggttc cgtgcgtacc ggtacgcctc gtggatgtac
      361 ggctcgggca tgtagtccgc gccgaagagc ttgaggtaga gctccggata ccggtagccg
      421 acggactcgt cggggaagaa ccgcagcgcc tggtgatagc gaatggccca gctgacctcc
      481 tcgtccacgt agggctcgag gagctgggcg ccccagtatc cgtggtcgcc gcggatgaac
      541 ccggcaatgg cgatgtcgtg cagcaggcac gcgaggacca ccttctcgct cgtgccgttc
      601 ttcagcgcga gccgcgccga ctgcaggagg tgcgcggtgg ggccgaagcg atggcggaag
      661 aagtcgaaga gggtgggctg gtccggcatc cgggacaggc gtgggtcgtg gcccatcatg
      721 tagcgcttgc cctggtccgg cggccgcgga atcggctgcc gcgggtcgcg ctcacccgag
      781 gtgtagatca cggacttcac gacgacccgg tccagctcct ggctcatcgc gtgctccgca
      841 gcctcggccc gctgtagcgt cgtcatctgc gtgtccccct gggcactcga gcgatgatca
      901 tgcgcgccac cgccgactgt caagttcggc ggccatccct tcggcgtgcc ggaccggcga
      961 tttcggccgg tgagggccga cggcggaggc gtctcagaag ctcacgggca gcgcttgtag
     1021 cccgcgcagc gtgaggctct ggcggtactc cggccggtcg accgccagcg caagcttggg
     1081 cagccgccgc acgagcgtgc cgatggcgat ctggccctcg aggcgcgcga gcggcgcgcc
     1141 gaggcagaaa tggatgcccc agccgaaggc gatgtggcgg ttgtcggcgc gagcgatgtc
     1201 caggcggtcg ggctcgggga actgcgcggg gtcgcggtcg gcagcgccga tgaacggcat
     1261 caccatctcg cccttgccga tcgtccggcc gccgatcgtg acgtcctcgc tcggaacccg
     1321 ggccgtccgc tgcacggggc cgtcgaagcg cagcagctcc tcgacggcgc tggcgaggag
     1381 cgacgggctc tcccggagcc gctgcagctc cccggggtgg cgcagcagcg ccagggtgcc
     1441 gttgccgatg aggttcaccg tcgtctcgtg gccggcgacg aggaggagga tgcacgtcgc
     1501 cagcagctcg tcctcgctca gcgtgtcgcc cgcctcctcg gcggcgatca gcgccgacag
     1561 cagatcgtcc cgcggcgcgg cgcggcgctc gacgatgagg ctgcggaagt agtcggacag
     1621 ggcgtggcgg gcggcggcgc tgcgccgcgg cacctcggag tccggcggca acaggatgga
     1681 atcgagccct cgggcgatgt cgaggcccca tcccttgaag cgctcgtggt cctcgacggg
     1741 aacgcccagc atctcgcaga tgacgatgac cgggagcggg taggcgaact gctcgatgag
     1801 gtccatcgag ccggcggctt cggcgcggtc gaggagtccg tcgacgatct gctggacgtg
     1861 cagccgcaag cgctgcacca cacgcggggt gaaggccttg ctcacgaggc tgcgcagccg
     1921 cgtgtggtcc ggtgggtcgc gatcgagcat cgagagcccg atccccggcg gcaccgccac
     1981 gccgaagcgg gccgcgatga gcgcggcgat cggttccttg acgcagcgcg ggtcgcgcag
     2041 caccgccacc acgtcctcgt agcgggtgag cacccagaag ccgagcgggc tgtggtggac
     2101 gggatcctcg gcgcgaaggc ggtgataggt cggatagggg tcggccagga actccgggtc
     2161 catcgggttg aagccgatca tcggagcctg ggcagcagcc gcgcggccgc ctcgtccacc
     2221 tgggcgagct ggtcgccgcc ggcgaagcgc aggctgatgt ggcgcgcgcc cgcgtcgatc
     2281 cagcgctgga gccactcgac gcagccctcg accgggccgg cgtaggtggc ctggcgggcc
     2341 aggatcgctc gcgccggcgc cgcgtagtac gtttcgagga acgcgtgcag ccgctgctcg
     2401 gcggcggcgc gactggcatc gagcgcgagg gtgacgtagg ccgcgccggt gacgtcctcg
     2461 gacgcccgcg cggcggcgcg cgcggccgcc cgcacgcgag ggaagtgctc ggcgaagaac
     2521 tcgacgctgg ggccggtggg gaaccaggca tcgaaacgcg cggcctcgcg cagcgccgtc
     2581 gggccgctgc cgccgatcca gatgggcggt ccgccgggac ggtggggctt gggctccagc
     2641 gtgacgtgcg ccagcgtgaa gtgcttgccg ttgaagctca cgtcgtcgcg cccccacagc
     2701 gcacggcaga tctcgagcgt ctccagaaag cgtccgacgc ggcgctcgaa gggcacgccg
     2761 gcctcggcga actccttgcg gatggccggc gtatccgcgg cgatgccgac gccgaggatc
     2821 acccgtccct cggcgatgcg gtcgagggtg ccgacgacgt gggcgaggac gacgggattg
     2881 cgcagcaccg gcagcagcac gccggtgccc agacgcacac gtcgcgtgcg cgcggccacc
     2941 gcggccagca gggtcagcgg ctcgtgacgc gggcgcgcgg tcagcgagtc gccgatccac
     3001 acggaatcga agccggcggc ctcggcgcgc tcggccatgg ccaggagcgg cgccgtgtcc
     3061 gggcggccgg acatgatcgc ttcgcgcgtg ggcaacagta caccgaactc gacggccatc
     3121 gagaactcct tctgtgacgc ttttcttacc cgccgccgct gcgccaggac ccagcgtgtc
     3181 ggagtcaccg tggttcgagc gccggccccg acggcgcaat ccccgttctg gggagtctcg
     3241 gagggggctg tcgaggcccc gtccgacgtc tatcgccgtg cgatcaggcg ccggaagacc
     3301 gcaccgtatt cgaagccgta gcccgctgcg tgggccgcgg cgttcttctt cacgcgctcg
     3361 ccgatggtct ggcccgccgg cacgttgaag cccggcacct ggctctcgtg gcggatcagg
     3421 gcggcgatct gccgctcgat cgagtccgag acgtccacga tgacgtccgg ttcgtccatg
     3481 ccccagtacc agagctcccg cacccgatga ggctcgagcc cgtccttcgc gatgtgctcg
     3541 gggaagtgca ggtggtcgcg ggcgtaggga tagacggcgt ccgtcgtcac gaggccggcc
     3601 ttgcggtgat cgcggtgctg gaagcccttg atccggtacg gatcgtggac gaagaccgtg
     3661 tgcggccggt accgccggag ggcgcgcacg atgtcgccga ggacctcgcg ggtgtcctcc
     3721 agctcgccat cgggatagcc gagcatcacg acgtgcttca cgcccatggc gtccgcggcg
     3781 gcgcgctgct cgtcggcccg cttcttcgcc agcgcctcgg gcgacagcga tcggtcggcg
     3841 gtgccggcat ggccgttggt gcacagcacg taggtcacct cgcagccggc ggccacccac
     3901 ttcgcgacga cgccgccgga tccgatctcg gcgtcgtcag gatgcgcgaa gaccaccatc
     3961 gcccggtcgg ggacgtcggt cacgacctct agcttgcggg cggcgtcggg tggcatgccg
     4021 ggccattcta ccggacgagg caagccctcc gcgccgcccg cgaagcgacc tacttcggct
     4081 gcgccaccgt ccgcaggtac ggcttgtggg tcttgaagcc ctgcgggtac ttcttcttcg
     4141 cctcctcgtc ggacacgttc gtggggatga tgacgtcctg ccccggcttc cagttgaccg
     4201 gcgtggcgac ggcgtgcttg gcgttgagct gcagcgagtc gagcagccgg agcacctcgt
     4261 cgaagttccg tccggcgctc atcgggtaga caagcatggc cttcaccttc ttgtcgggcc
     4321 cgatgatgaa gaccgagcgg atggtggcgt tgtcgatcgc ggtgcgctgc ccgccggccg
     4381 cgttggggtg gatcatgtcg tacagcttcg ccaccgtgag gtcggcgtcg ccgatgagcg
     4441 gatagttgac ggcgtggccc tgcgtctcct ggatgtcctt gacccaggcc ttgtggtcgc
     4501 cgaccggatc gacgctcagg ccgatgatct tcgtgttgcg cttgtcgaac tcgggcttca
     4561 ggccggccat gtagcccagt tcggtggtgc agaccggggt gaagtccttg ggatgcgaaa
     4621 agaggatggc ccaccggtcg ccgatccact cgtggaaccg gatcttgccc tcggtcgtct
     4681 cggcggtgaa gtccggggcc tcgtcaccga tgcgaactgc catggcgttc tccgttctcg
     4741 ttggggttga cggatacgaa caaccagcgt ctcggagcag agattatagc gccgtcgacc
     4801 agcttgattc caacgcgccg agggcgcatc ctcgccacca ccaatcaccc ggagggcgaa
     4861 acggcgatga agatcagcga ggtgcaggcc acgtgggtcc acgttcccat cccccacgag
     4921 cggcagcacg tgagcgactt cggccgcgtc gcgtcgttcg actccgtggt ggtgaaggtc
     4981 gtgaccgacg gcgggctcgt gggctggggc gaggccaagg cgggggtcgg cagcgcggcc
     5041 tccgcgcacg ggctcgccgc catcatcggc cgcgactacg ccccgctgct ggtggggcag
     5101 gacccgcgcg acgtgagccg cctctgggac gtgatgtaca acaccccgcg ggagggctat
     5161 gcggtcgatc gcggccacgt gctgccccaa ctcgggcggc ggggcctgtc gatcccggcg
     5221 atcgccggtg tggacatcgc cctctgggac cttctcggca agtcgctcgg agctcccgtc
     5281 tggcgcctgc tcggcgggcg ccgtgccgag cggatgcccg cgtacgcgtc gggcggctgg
     5341 gccgacgagg cccgcatcgg cgagcagctc ctcggctacg tcgagaaggg cggcttccgc
     5401 gcggtgaaga tgcgcgtcgg cgtgatggac ggcgagccgc atcggtcggc ggcgcgggtg
     5461 cgggcggcgc gcgcggcgat cgggccggac atcaagctga tggccgatgc ccacggcacg
     5521 tggacggtct cggaggccaa ggcgttctgc cggatggtcg aggactgcga cctctactgg
     5581 ctcgaggagc cggtgacggc cgacgacaag accgggctcg ccgaggtgcg ccgcgcctcc
     5641 gccgtgccga tcgccaccgg cgagagcgag ttcacccgcc acgacttccg cgagatcgcc
     5701 gagctgcgcg ccgccgacat cctgcagccg gacctcgcga tcgcgggggg gattaccgag
     5761 ggagtgcgca tcggcgccat cgcctccgcc tacaacctgc gattggcgcc gcacctctgg
     5821 tcgggcgcgc cggcgttcgc ggcgggcgtc gcgctggccg ccagccagag cgcgggcttc
     5881 atcctcgagt actcgctcgg cgccaacccg ctgttgcacg agctggtcca cgagcagttc
     5941 ccggtgatgg acggccacgt cgagattccc gaccgccccg gcctgggcat cacggtcgac
     6001 gaggacttcg tgcgtcgtca cgcgcggcac tgacgcgcga ggcggacggc gaacgtgtat
     6061 catccgtctc cggccacacc gatgaggcgt cgaggaggag cacgatgaaa gtcaaggcgg
     6121 tgaacggctt cgcgcggatc ctcaaggccg agggcatccc gtgggtgagc tgctacccca
     6181 ccagccccgt gaacaacgcg ctcggcgagg aaggcgtgcc catcctcatg atgggggagg
     6241 agcgcttcgc cgtcgccgtc gccgacgcgt tctcccgcgt gaccagcggc aagcagatcg
     6301 gggcgtgcac cgtcatggcc ggcctcaacg ccgcgggcat ccagatggcc tacgg
//
LOCUS       DATIAZ010000303         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1139445, whole genome shotgun sequence.
ACCESSION   DATIAZ010000303 DATIAZ010000000
VERSION     DATIAZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139445"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VLF19_10560"
     CDS             complement(<1..675)
                     /locus_tag="VLF19_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93738.1"
                     /translation="MIRRAYLLLPGAVLLAVASLPFFVPEWREAAGPLAPLAGWVAIG
                     TGILLGLRFGHVTVVVALVVLALADQAVTRWGEVGNLREVVALLTPLNLAGLAWTSDR
                     GARRYRVKLWTGLLAIQACGVAGHIFFQPIRLPWPAIGPPSVLAFAVALAATLARFAV
                     RPRAVEAALVWAVAATFLAFGADGDPVVRSLQFAAGALALVVALVETSHTMAYGDELT
                     GLPGRRA"
     gene            complement(672..1106)
                     /locus_tag="VLF19_10565"
     CDS             complement(672..1106)
                     /locus_tag="VLF19_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSE93739.1"
                     /translation="MARLTLAQSTRIVEVALARGRELGCAPLAVAVLDAGGHLKAFAR
                     EDNAGILRPRIAIGKAWGALGMGFGSRVLAKRATDNPAFFAALYAASDGQLVPAAGGV
                     LVRDAAGEVLGAVGISGDTSDKDEACAIAGIEAARLVAEPGA"
     gene            1279..2844
                     /locus_tag="VLF19_10570"
     CDS             1279..2844
                     /locus_tag="VLF19_10570"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HSE93740.1"
                     /translation="MAWSLGGIVREHARRRGDRPMITFGERTITWAEMDARSSRVAQA
                     MLAAGLGAQDRVAFVDKNGPEYFEVLFGGGKAGVVDVAVNWRLAPAEMAYVVNDAEAK
                     LLFVGPDFLDHLDAMEGSLKSVRQVIVLATRPRRVAHVGYEAWVSAHPATDPDRPSPP
                     GDVAMQLYTSGTTGLPKGAMLTGANLGMLIPNVGSWWDLDQTSVNCVCMPLFHIGGSG
                     WALVGMAQGGHSILFREFVPQEILAALVRHRITNALFVPSMLQFLSAVPGAAERDYSA
                     LRSIVYGASPITNEVLLRSMTTFRCKFVQVYGLTETTGAITQLPPGDHATAGPRARLL
                     RSAGKPFPWVELRVVDPATGADCAPGAVGELWTRSPQNFRGYWNRPDETARTLTPDGW
                     LRTGDAGFLDAEGYLFLTDRVKDMIISGGENVYPAEVENALSEHPDVADVAVIGVPDE
                     TWGETVKAIVVPKPGVTPRAEDIIAFTRVRLAHYKCPTSVDFARALPRNPSGKLLKRE
                     LREPYWKGRERRI"
BASE COUNT          434 a         1061 c         1061 g          383 t
ORIGIN      
        1 cgcgcgccgg cccggcagcc cggtcagctc gtcgccgtac gccatcgtgt gcgacgtctc
       61 gacgagggcg accacgagcg ccagcgcgcc ggcggcgaat tgcaggctgc gcacgaccgg
      121 gtcgccgtcg gcgccgaagg cgaggaacgt ggccgcgacc gcccacacga gggcggcctc
      181 gacggcgcgt gggcggacgg cgaagcgcgc cagggtggcc gcgagcgcca cggcgaacgc
      241 gaggacggaa ggcggaccga tcgccggcca cggcaggcgg atcggctgga aaaagatgtg
      301 cccggcgaca ccgcatgcct ggatcgcgag cagccctgtc cagagcttca cacggtaccg
      361 ccgcgccccg cggtccgacg tccatgccag cccggcgaga ttgaggggcg tgagcagcgc
      421 gacgacctcg cgcaggttgc cgacctcgcc ccaccgcgtc accgcctggt cggcgagggc
      481 gagcacgacg agcgccacga ccacggtgac gtggccgaac ctgagaccga gcaggatgcc
      541 ggtgccgatg gcgacccagc cggcgagcgg ggcgagggga ccggcggcct cgcgccactc
      601 ggggacgaag aagggaagag acgcgacggc gagcagcacc gcgccaggca gcagcaggta
      661 cgcgcgtctg atcaagcgcc gggttcggcg acgagacggg cggcctcgat accggcgatc
      721 gcgcacgcct cgtccttgtc ggacgtgtcg cccgagatgc cgaccgcacc cagcacctcg
      781 cccgccgcgt cgcggacgag cacgccaccc gcggccggca ccagctggcc atcggaggcc
      841 gcgtagaggg ccgcgaagaa cgccgggttg tcggtggcgc gcttggccag cacccgcgag
      901 ccgaacccca tgcccagcgc gccccacgcc ttgccgatgg cgatgcgcgg gcgcaggatg
      961 cccgcgttgt cctcgcgcgc gaaggccttg aggtggccgc cggcgtcgag cacggccacg
     1021 gcgagcggcg cgcagcccag ctcgcggccc ctggcgagcg cgacctcgac gatccgcgtg
     1081 gattgcgcga gcgtcagtct cgccatctct gttcgagaat accagagccg cccttgtttc
     1141 agttccgcct tctccgcctt atcgcgcgcg gcggccaggc gaccggctcg accgcaccgg
     1201 cgagaggggc gggtgggcta cactcggggc ctccggcccg gggccggcag ccgcccacga
     1261 caggagggat ctcgagcgat ggcatggtca ctcggcggca tcgtcaggga gcacgcccgc
     1321 cggcgcggcg accggccgat gatcaccttc ggcgagcgaa cgatcacgtg ggccgagatg
     1381 gacgcgcgct cgagccgcgt ggcccaggcg atgctcgccg ccgggctcgg cgcgcaggac
     1441 cgcgtggcct tcgtggacaa gaacggcccg gagtacttcg aggtgctctt cggcggcggg
     1501 aaggccggcg tcgtcgacgt cgccgtcaac tggcggctgg cgcccgcgga gatggcctac
     1561 gtcgtcaacg acgccgaggc gaagctgctc ttcgtcgggc cggacttcct cgaccacctc
     1621 gacgcgatgg agggctcgct caagagcgtc cggcaggtga tcgtgctggc gacgcgtccg
     1681 cggcgcgtcg cgcacgtcgg ctacgaggcg tgggtgagcg cgcatccggc gaccgacccc
     1741 gatcgtccct cgccgcccgg tgacgtagcg atgcagctct acacctcggg gacgacgggg
     1801 ctgcccaagg gcgcgatgct gaccggcgcc aacctgggca tgctgatccc caacgtcggg
     1861 tcgtggtggg acctcgacca gacgtccgtc aactgcgtct gcatgccgct cttccacatc
     1921 ggcggctcgg gctgggccct cgtcggcatg gcgcagggcg gccactcgat cctcttccgc
     1981 gagttcgtgc cgcaggagat cctcgcggcg ctcgtgcgcc accggatcac caacgcgctc
     2041 ttcgtgccgt cgatgctgca gttcctctcc gccgtaccgg gtgccgccga gcgcgactat
     2101 tcggctctgc gctcgatcgt gtacggtgcc tcgcccatca ccaacgaggt cctgctgcgc
     2161 tcgatgacga ccttccgctg caagttcgtg caggtctacg gcctgaccga gacgaccggc
     2221 gccatcaccc agcttccgcc cggcgaccac gccaccgcgg ggccgcgcgc ccggctgctg
     2281 cgctcggccg gcaagccgtt cccctgggtc gagctgcgcg ttgtcgatcc ggccacgggc
     2341 gcggactgcg cgcccggcgc cgtcggcgag ctgtggacgc gctcgcccca gaacttccgg
     2401 ggctactgga accgtcccga cgagaccgcg cgcacgctga cccccgacgg ctggctgcgg
     2461 accggcgacg ccgggttcct cgacgcggag ggctacctgt tcctcaccga ccgcgtgaag
     2521 gacatgatca tttcgggcgg cgagaacgtg taccccgcgg aggtcgagaa cgcgctctcc
     2581 gagcaccccg acgtcgccga cgtggccgtg atcggcgtgc ccgacgagac gtggggtgag
     2641 accgtgaagg ccatcgtggt gccgaagccg ggcgtcacgc cacgcgccga ggacatcatc
     2701 gcgttcaccc gcgtgcgcct ggcccactac aagtgcccga cctcggtgga cttcgccagg
     2761 gcgctgcccc gcaacccgtc gggcaagctg ctcaagcgcg agctgcgcga gccctactgg
     2821 aaggggcgcg agcggcggat ctaattactt tccggtgcag aaatgggcgc tgagaccagg
     2881 tggggcgcgc tgggaagagg tcggattccg gtcagacctc tcacttcggg tgcttctgg
//
LOCUS       DATIAZ010000304         5710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_877358, whole genome shotgun sequence.
ACCESSION   DATIAZ010000304 DATIAZ010000000
VERSION     DATIAZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877358"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..460)
                     /locus_tag="VLF19_10575"
     CDS             complement(65..460)
                     /locus_tag="VLF19_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93741.1"
                     /translation="MTSESSPSPPPPTGRRILLVEDSADARDALRLLLELDGHAVSVA
                     ADGSEALARAAEVEPEVALIDIGLPVLDGYDVARRLRAMPLGSKMRLIALTGYGQPED
                     QARVLEAGFDGHLVKPVDPEMLSRHLAAQ"
     gene            complement(532..1236)
                     /locus_tag="VLF19_10580"
     CDS             complement(532..1236)
                     /locus_tag="VLF19_10580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HSE93742.1"
                     /translation="MSVTAPKRNHVRAMFTRIAGRYDLMNSIMTGGRHHAWRRAVARA
                     AVAAPPGPILDLATGTGDLALEILALDPARTVVGADFSEGMLRAAAAKLAARRAPRLP
                     LLSADALALPFADGTFACVTSAFLLRNLEDLGSGLAEMRRVTQRGGRVIALDIVRPAT
                     PVWDRVFALYFHRVVPAVGTLVARDRAAYTYLPASVARFVTPAELARLMARADLRDVT
                     YRRLGLGTVALHLGFA"
     gene            complement(1233..2111)
                     /locus_tag="VLF19_10585"
     CDS             complement(1233..2111)
                     /locus_tag="VLF19_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HSE93743.1"
                     /translation="MEGLRALSAVVVRVGHVLDAIKFEHTVFALPFAYISMVLAAGGW
                     PGWWTVAWVTVAMAGARTCAMAANRVIDRATDAKNPRTAGRHLPRGLLRAWQLGALAA
                     GGAALMVVAAWMLNPLCLALAPIALVFLVGYSYTKYVTWTSHWVLGFTDGIAAGGGWI
                     AVTGGIAAPALVLWFALTVWIAGFDLIYACQDVAFDRAHGLRSVPARFGVAAALRIAR
                     VNHALTAVALAALGWMTALGPLYWLGWLAIAALLAYEDALVRPDDLSRLGVAFFNVNG
                     YIAVLALAAVVAGMLW"
     gene            complement(2090..3547)
                     /locus_tag="VLF19_10590"
     CDS             complement(2090..3547)
                     /locus_tag="VLF19_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HSE93744.1"
                     /translation="MAWRDLREFVDHLERRGRLRRVAVPVSPELEITEIADRVSKGPA
                     RDNVALLFERVEGSDVPVLVNAFGAADRMAWALGVERLDELGERVAKLLDLRVPGTFA
                     ERLGKLGTLIDLVKAAPRRVQSAPCQEVVETAQPTLASLPVLTCWPGDAGPFITLPSV
                     FTRDPLTGARNVGMYRLQRFDDRTLGMHWQTHKGGAEHQRRAAESGEQARIPVAIALG
                     GDPAVTYAASAPLPPGVDEIVFAGWLRGGGVEMVECRTVDLEVPAQAEIVLEGWVDPR
                     EHRREGPFGDHTGYYSIARDYAVFHLTAVTRRGRPIYPTTIVGRPPQEDYWLGKATER
                     LFLPVIRMMLPEVVDVNMPAEGVFHNLVIVSIRKRYPGQARKVMTALWGMGLMALAKT
                     IVVVSEHVNVHDLSEVAWRATGNIDPRRDLMVLEGPMDDLDHAALRHRFGGKLGVDAT
                     EKGPMDDVAQPWPEEIVMSDEVRERVTRRWKDYGL"
     gene            complement(3690..4055)
                     /locus_tag="VLF19_10595"
     CDS             complement(3690..4055)
                     /locus_tag="VLF19_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93745.1"
                     /translation="MRVLVVEDVWDILEVFSILLRAEGAQVATAGTGQEAAELARQGD
                     FDVVLTDLGLPDIPGDVLIRQLRGGGKARARVVAVTGFGEPYISRALQAGADVVFTKP
                     VEWNRILEYLSRPDLAASA"
     gene            4177..>5710
                     /locus_tag="VLF19_10600"
     CDS             4177..>5710
                     /locus_tag="VLF19_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSE93746.1"
                     /translation="MRPPSGADYLVSLADGIESALVVVDTELTVVHWNAAMERLTRLP
                     RAEACGHRLGGLTDALDRIGLVERLETALAGEVRYGATLPGGTPVEARCQPLHDETGQ
                     TVGAAAFLFDVTERRRREVFVRAMRAIGRSLTSSLDLNEALDTIVSRTLEVMAADSAL
                     VLSWDGVADELRVLRAAGRLSEHHARSGAVPVSGGPVSRAVREGKAVTTPNILSDSRL
                     WLTPERQAQIEREGFKAVAAAPLASKGRVHGALVVHYWAEREVSAEELTALTLLAEQA
                     AIAIDNARLYADATRRAERLRELAEVERLVGGSLDLDDVLLRIAEATARLVGAPVVHL
                     WTVEPGAAVLRLRAASVDPSMPAVDMPATCAFGEGISGMAAQSRSPVFVPDAAGDPHV
                     RTPGWVHAGLRTVLSVPILSGETLLGVLTVRARPGALSADEDQALVTSLAARAAVAVQ
                     NARAYQEAVRRGARLRELAAVNQSITASLDAADVMQRIAAGAASFAPGAMAAVHVFEP
                     AQG"
BASE COUNT          837 a         2026 c         2105 g          742 t
ORIGIN      
        1 cgaccccgcc ctggcctacg gtcggcgagc gcgcggctcg ggactcagga ctcgcgactc
       61 aggactactg tgcggcgagg tggcgggaga gcatttccgg gtcgacgggc ttgacgaggt
      121 ggccatcgaa gccggcctcc agcacgcgag cctggtcctc gggttgtccg tagccggtca
      181 gggcgatgag acgcatcttg ctgccgagcg gcatggcccg cagcctccgc gccacgtcgt
      241 agccgtcgag caccggcaac ccgatgtcga tcagggcgac ctcgggctcg acctcggccg
      301 cgcgcgccag ggcctccgag ccgtcggcgg cgacggagac cgcgtgaccg tcgagctcga
      361 gcagcagtcg cagcgcatcc cgtgcgtcgg cgctgtcctc gacgagcagg atacgccggc
      421 cggtcggagg cggcggagac ggactggatt cggaggtcat ttctccgtac gacgcctcac
      481 agtctaccag cgtgtgccgc caccacaaga ccgacccacg tattataaag gttacgcaaa
      541 tcccagatgc agggcgaccg tgccgagccc gagccggcga tacgtcacat cgcgcaggtc
      601 cgcgcgcgcc atgagacgcg cgagctcggc cggcgtcacg aagcgggcca cggaggccgg
      661 gaggtatgtg taggccgcgc ggtcgcgcgc gaccagcgtg ccgaccgcgg gtaccacgcg
      721 gtggaagtac agcgcgaaga cgcggtccca caccggcgtg gccggccgca cgatgtcgag
      781 ggcgatgacg cgtccgcccc gctgcgtgac tcgccgcatc tcggccagcc cacttcccag
      841 gtcctcgaga ttgcgcagga gaaacgcgga ggtcacgcag gcgaacgtcc cgtcggcgaa
      901 cggtaacgcc agcgcgtccg cggagagcag cggcaggcgc ggcgcccggc gggcggcgag
      961 cttcgccgcc gcggcgcgca gcatgccttc cgagaagtcg gcaccgacga cggtgcgcgc
     1021 cggatcgagc gcgaggatct cgagggccag gtcgccggtg cccgtggcca gatcgaggat
     1081 cggccccggc ggcgcggcga cggcggcccg cgcgacggcc cggcgccagg cgtggtgacg
     1141 cccgccggtc atgatgctgt tcatgaggtc gtagcgtccg gcgatgcgcg tgaacatcgc
     1201 gcggacgtgg ttgcgcttcg gcgcggtcac gctcaccaga gcatcccggc gacgacggcc
     1261 gcgagggcga ggacggcgat gtagccgttg acgttgaaga aggccacccc gaggcgcgag
     1321 aggtcgtccg ggcgaacgag cgcgtcctcg taggccagga gcgcggcgat cgccagccat
     1381 ccgagccagt agagcggccc gagcgcggtc atccatccga gcgcggcgag ggcgacggcc
     1441 gtgagcgcgt ggttcacgcg cgcgatccgg agcgccgcgg cgacgccgaa gcgcgcgggc
     1501 accgagcgca ggccgtgcgc ccggtcgaag gccacgtcct ggcaggcata gatgagatcg
     1561 aagcccgcga tccagacggt gagcgcgaac cacagcacga gggccggcgc ggcgatgccg
     1621 ccggtgacgg cgatccagcc gccgccggcc gcgatgccgt cggtgaagcc gaggacccag
     1681 tgcgacgtcc acgtgacgta cttggtgtac gagtagccga cgagaaagac gagcgcgatc
     1741 ggggccaggg ccagacacag cggattcagc atccaggcgg cgacgaccat gagggcggcg
     1801 ccccccgcgg caagggcgcc gagctgccac gcccgcaaca ggccgcgtgg gaggtggcgg
     1861 ccggcggtgc gcgggttctt cgcgtcggtg gcgcggtcga tcacgcggtt ggcggccatc
     1921 gcgcacgtcc gcgcgccggc catggccacc gtcacccacg ccaccgtcca ccaccccggc
     1981 cagccgccgg cggccagcac catcgagatg taggcgaagg gcagggcgaa gacggtgtgc
     2041 tcgaacttga tcgcgtcgag gacgtggccg accctgacga cgacggcgct cagagcccgt
     2101 agtccttcca gcgccgggtg acgcgctcgc gcacctcgtc gctcatgacg atctcctcgg
     2161 gccagggctg ggccacgtcg tccatcggtc ccttctcggt ggcgtccacg ccgagcttgc
     2221 cgccgaagcg gtggcgcagg gccgcgtgat ccagatcgtc catcggcccc tcgagcacca
     2281 tgaggtcgcg ccgcggatcg atgttgcccg tggcgcgcca ggccacctcg gagaggtcgt
     2341 ggacgttcac gtgctcgctc accaccacga tggtcttggc cagcgccatg agccccatgc
     2401 cccagagcgc cgtcatcacc ttgcgcgcct ggccgggata gcgcttcctg atcgagacga
     2461 tcacgaggtt gtggaacacg ccctcggcgg gcatgttcac gtcgacgacc tccggcagca
     2521 tcatccggat caccggaagg aacagccgct ccgtcgcctt gccgagccag taatcctcct
     2581 ggggcggccg gcccacgatc gtggtcgggt agatcggccg cccgcgccgg gtgacggcgg
     2641 tcaggtggaa cacggcgtag tcgcgggcga tcgagtagta gcccgtgtgg tcgccgaacg
     2701 gcccctcgcg gcggtgctcg cgcgggtcca cccagccttc cagcacgatc tcggcctggg
     2761 ccggcacctc gaggtcgacg gtgcggcact ccaccatctc cacgccaccg ccgcgcagcc
     2821 agccggcgaa cacgatctcg tcgacgccgg gcggcagcgg cgccgaggcc gcgtacgtca
     2881 ccgcggggtc gccgccgagc gcgatcgcca cggggatgcg ggcttgctcc cccgattccg
     2941 cggcgcggcg ctggtgctcg gcgccgccct tgtgggtctg ccagtgcatg ccgagcgtgc
     3001 ggtcgtcgaa cctctgcagg cgatacatgc cgacgttgcg cgcgccggtc aggggatcgc
     3061 gcgtgaacac ggacggcagc gtgatgaacg ggcccgcgtc gcctggccag caggtgagaa
     3121 cgggaaggct agccagcgtt ggctgcgcgg tctcgaccac ctcctggcac ggcgcggact
     3181 ggacgcgccg cggcgccgcc ttcacgaggt cgatcaacgt gccgagcttg ccgagccgct
     3241 cggcgaacgt gccgggcacg cgcaggtcga gcagcttcgc cacgcgctcg cccagctcgt
     3301 ccaggcgctc gacgccgagt gcccaggcca tgcggtccgc agcgccgaag gcgttgacga
     3361 ggaccggcac gtcgctgccc tcgacgcgct cgaagagcag ggccacgttg tctcgcgcgg
     3421 ggcccttgga cacgcggtcg gcgatctccg tgatctcgag ttccggagac accgggaccg
     3481 cgacgcggcg caggcggccg cgacgctcga ggtgatcgac gaactcgcgc agatcgcgcc
     3541 aggccatggc gcgccggtca ctcctggtgg aaggtcgaga cgagcaccga aggatggaga
     3601 gcggggtgca tcgcggctcc ttttcggcac attattgcac acacgtccgg gcgggcttcg
     3661 ggcgagacgt gccgcgggag ccccaggcgt caggccgagg ccgcgaggtc tggacggctc
     3721 aggtactcga ggatgcggtt ccactcgacg ggctttgtaa agactacgtc ggcacccgcc
     3781 tggagggcgc gcgagatgta aggctcgcca aagccggtca ccgccacgac gcgcgcgcgg
     3841 gccttgcccc cgccacgaag ctgtcggatg agcacgtcgc ccgggatatc tggcaggccg
     3901 aggtcggtca gcacgacgtc gaagtctccc tggcgcgcca gctccgcggc ctcctgaccg
     3961 gttccggccg tcgccacctg ggcaccctcg gcgcgcagga ggatactgaa gacttccagg
     4021 atgtcccaga cgtcttcaac cacgaggacg cgcactccat ccagtgcggg cgcccgatgc
     4081 acagccaaga aggccccctc tccaacttca tcggggagca agcgcggtgc catttgaccc
     4141 gtcccgcggc ccccctcgcc gtatactgga cgagtcatgc ggccgccctc gggagccgac
     4201 tatctggtca gtctggccga tgggatcgag tccgcgctcg tggtcgtcga taccgagctc
     4261 acggtcgtcc actggaacgc cgccatggag cggctcaccc gactgccccg ggccgaggcg
     4321 tgcggtcacc gcctgggcgg gctgaccgat gcgctcgacc ggatcgggct cgtcgaacgc
     4381 ctcgagaccg cgctggccgg cgaggtgaga tacggcgcga cgctgcccgg cggcacgccg
     4441 gtggaagcgc gatgccagcc gctgcacgac gagacggggc agacggtagg cgccgcggcc
     4501 ttcctgttcg acgtcaccga gcgccggcgc cgcgaggtct tcgtgcgcgc gatgcgcgcg
     4561 atcgggcgct cgctcacgtc gtcgctggac ctcaacgagg cgctcgacac gatcgtgagc
     4621 cggacgctgg aggtcatggc cgcggactcc gcgctggttc tctcgtggga cggcgtggcc
     4681 gacgagctgc gcgtgctgcg cgccgccggg cgcctgagcg agcaccacgc gagatccggc
     4741 gccgtccccg tgtcgggcgg gccggtcagc cgcgccgtcc gcgagggcaa ggccgtcacg
     4801 acgccgaaca tcctgagtga ctcgcggctc tggctcacgc ccgaacgcca ggctcagatc
     4861 gagcgcgagg gcttcaaggc cgtggccgcg gcgccgctcg cctccaaggg acgggtgcac
     4921 ggggcgctcg tcgtgcacta ctgggcggag cgcgaggtga gcgccgagga gctgacggcc
     4981 ctgacgctcc tggccgagca ggccgccatc gccatcgaca acgcgcggct ctacgccgac
     5041 gctacccggc gcgccgagcg gctgcgcgag ctggcggagg tggagcggct cgtcggcggc
     5101 tcgctcgatc tggacgacgt gctgttgcgc atcgccgagg cgaccgcgcg gctcgtgggc
     5161 gcgccggtcg tgcatctctg gacggtggag ccgggcgccg cggtgctgcg cctgcgcgcg
     5221 gccagcgtgg acccgtcgat gccggccgtc gacatgccgg cgacgtgcgc cttcggcgag
     5281 ggcatcagcg gcatggccgc ccagagccgg tcgcccgtgt tcgtgccgga tgccgccggc
     5341 gacccgcacg tgcgcacgcc cggctgggtg cacgcgggac tccggacggt gctgtcggtg
     5401 ccgatcctct ccggcgagac gctgctcggc gtgctgaccg tgcgtgcgcg gcccggcgcg
     5461 ctgtccgccg acgaggatca ggccctggtc acctcgctcg ccgcgcgcgc cgccgtggcc
     5521 gtccagaacg cgcgcgccta ccaggaggcg gtgcggcgcg gagcgcggct gcgcgagctg
     5581 gccgcggtga accagtcgat caccgcctcg ctggacgccg ccgacgtcat gcagcggatc
     5641 gccgccggcg cggcgagctt cgcgcccggg gccatggcgg ccgtgcacgt gttcgagccg
     5701 gcgcagggca
//
LOCUS       DATIAZ010000305        20096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1271126, whole genome shotgun sequence.
ACCESSION   DATIAZ010000305 DATIAZ010000000
VERSION     DATIAZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 20096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20096
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271126"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VLF19_10605"
     CDS             <1..1148
                     /locus_tag="VLF19_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HSE93747.1"
                     /translation="GRGRKARVAYGFDDIALVPGAVTVNPGEVDISWELCGRRFELPI
                     IAAAMDGVVSPPLAIEMGRLGGLAVLNLEGIFSRYENPDDVLDRIVSASQAEATKIIQ
                     SIYGEPIKEELIHRRIGEIKKGGSAVAVSSIPQRAERFAQIAQEAGADVFIVQSTVTT
                     ARHIATEYAPVDLRRLKKQLAIPLVIGNCVTYEACLELMECGADALLIGVGPGAACTS
                     REVLGLGVPQVTATADSAAARDFYYKQTGRYVPIVTDGGMTTGGDVCKALASGADAVM
                     IGSAFARAVEAPGRGYHWGMATPHQNLPRGTRIRVGIAGSLEQILFGPAFTEDGTLNL
                     VGAIRTCMGSVGARSIRELQQTELIIAPAIKTEGKVFQQAQKLGRPR"
     gene            1171..2721
                     /gene="guaA"
                     /locus_tag="VLF19_10610"
     CDS             1171..2721
                     /gene="guaA"
                     /locus_tag="VLF19_10610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSE93748.1"
                     /translation="MDAADKIAILDFGGQYTQLIARRIREMGVYSEILPCTQPLESVL
                     AGGYRGIVLSGGPSSVYEDAAPLPPKALFEAGVPVLGICYGMQAMGYLLGGHVVPAER
                     REYGPAEVALQGSSPLFAGVTPEASGRVAVWMSHGDNVLKPPKGFVSLGTTPNCPIAA
                     MADEQRRLYAVQFHPEVAHTPQGRRIIENFLGVCRARATWSMASFIETTVAEIRAKVG
                     QDRVLCALSGGVDSSVVAVLVHRAIGDQLTCMFVDNGLLRKGEANAVVHTFRDAFKLN
                     LVHVDASRRFLDALRGVTEPELKRKRIGAEFIAVFEEEARRLGEIPWLAQGTLYPDVI
                     ESISFKGPSATIKTHHNVGGLPTKMRFRLLEPLRELFKDEVRRVGELLGLPSEIVWRQ
                     PFPGPGLAIRVLGEVTEERLDLLRAADAVVQEEVRAAGLERELWQAFAVLLPVKTVGV
                     MGDFRTYAHVVALRAVMSQDAMTADWARLPYDVLGRISNRIINEVKGINRVVFDISSK
                     PPSTIEWE"
     gene            2738..6670
                     /gene="dnaE"
                     /locus_tag="VLF19_10615"
     CDS             2738..6670
                     /gene="dnaE"
                     /locus_tag="VLF19_10615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSE93749.1"
                     /translation="MTHSEFVHLHVHSEYSLLDGAAQLEKLVAKAKELKFPALALTDH
                     GNLFGAVDFYLVAQKAGIKPIVGCELYVAPRGRKERGSEDGGYEGANHLTVLVRNLAG
                     YQNLIKLVSRAYLEGFYYKPRVDKELLAEHADGLLVLSGCLNSEVARLLAAGEIDRAR
                     EAAGWYQEVFGKDHYFMEVQAHGLDEQVKVTAATREIARAIGAPIAGTNDSHYLEAGH
                     ARAHEALLCIQTGATLLDPKRWRFSSEEFYVKSAEEMARVFAEIPDACRNTVAVAERC
                     NLTLEFGRFHLPRYVVPDGHTLDSYLEHLSRQGLRRRYGPTPGDAIEARLGHELRIIE
                     KMGFAGYFLVVWDFIHYARQQGVAVGPGRGSSAGSLVAYALGITNVDPIRYGLLFERF
                     LNPERISMPDMDIDFADDRRDEVIRYVAEKYGRDRVAHIITFGTLGAKAAIRDVGRVL
                     GMPYADVDRIAKLVPNFPLNITLDDAYQKSPPLAEMVRTQAGVKELWDIARALEGCTR
                     HASVHASAVVISDDPLDDHVPLYKDPKRPELITGYAMGPIEKLGLLKMDFLGLRTLTV
                     LANTVALIREARGLTLDLDALPLDDAKTYALLTDAKTFGVFQLESGGMRDALRGLRPE
                     RLEDVIAMVSLYRPGPMELIPEFINRKHGRAKITYEHPAMETFTRETYGIMVYQEQIM
                     QIASEMAGFTMGEADTLRRAMGKKDRDLMAQQRDKFLAGCAERGTRRLQAERVWELME
                     KFAGYGFNKCLTADTWIEMADGSRKPITAIRDGDLVLTKDGPHAARRIRPSGIRPVGR
                     LVLANGMTVRCTPDHPIFTQRGWVDAQELAGGDAVAVLDTVAAADLRVAARRAAPGRA
                     SVITETPIDWSAPASFTPDGVEPTYDFEVPGAGSFIANGIVVHNSHAAAYALVAYQTA
                     YFKANYPVEFMAALLTSEMGDSDKIVKYIEECRAMGIRVEPPDVNVSAVKFSVEGATI
                     RFGLAAIKNVGEAAMESILATRDREGRFQGLEDFCARVDLRLVNRRVLESLVKAGAFD
                     SLGLTRAHLLATVDAALESGQRQQRERAEGQGSFFDLLPGVAATPRTEPVAVTPEWDD
                     DQRLQFEKEVLGFYVSGHPLARYGPLVESLGITTTDDLAGKSAGSRVLLLGQVAALKE
                     TATKSGNRMAFVTLEDMAGTVEVTVFPEPFKSAAEHLRAREPVLVRGRVDDSERGRSV
                     LAEDVRLLERVLTDSGARAATTPGEPNAVRIRVAATEDPRAVLGSVREACAEHPGPVP
                     VFVHVLLPGREVVVRSRALAVDPAPPLLERLAALLGPTATVVEHAGGA"
     gene            6654..7619
                     /locus_tag="VLF19_10620"
     CDS             6654..7619
                     /locus_tag="VLF19_10620"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HSE93750.1"
                     /translation="MPEALEFEQPLLELEARITALQALDDPAQRDEIGRLEDRLARLR
                     QKTFAGLTAWQTTQLARHPRRPHMRDFCRLVFEDFIELHGDRLYADDQAIVGGLACFE
                     GYGVVVIGHQKGRETREKLARNFGMPHPEGYRKALRLMRLAEKFGKPVVSLIDTPGAY
                     PGIGAEERGQAEAIARNLREMAALRTPIVAVVTGEGGSGGALALGMGNRVLMLQYAVY
                     SVISPEGCAAILWGDAAKAPDAAEIMKITAPDLLRLGVIDGIVPEPAGGAHRDWDVTA
                     ANLKAALATHLADLKVKSPEALVTERYDKFRRIGAFEEEAVPGRR"
     gene            complement(7625..8614)
                     /locus_tag="VLF19_10625"
     CDS             complement(7625..8614)
                     /locus_tag="VLF19_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HSE93751.1"
                     /translation="MKLISHRPGESRQMVQCLELYYRQGRSQKDIARALGVSAATVSR
                     LLKRAFDEGLVRVELDLPRTQELEVALMQHFGLRDAVVVAAGGRGDVREELGVAAAGY
                     FEKTVTSGMRVGISCGFTLYHTVRALRERRLRDLALYPLSGESTLKLVDISPNTLVGM
                     MAAKYRPHVTAYALPVQHLISLRQIERERRRLLRDPEVRRIYETARSVDIALVGVGQI
                     AEQTPGFCSLAESYGVSVKRLREFGVVGEINYQPVDAEGRIVAEPELRALMHRVLSVE
                     GERLQELSRREDRWVVAVAGGRSKTDAVRGALRGRFMNVLVTDEDVAQAALKP"
     gene            8725..10242
                     /locus_tag="VLF19_10630"
     CDS             8725..10242
                     /locus_tag="VLF19_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HSE93752.1"
                     /translation="MAGKRYGSGNGARGPALGDYHQSKDELAGLPQTVAEKIPVTNSP
                     NIFSYRAYVFAKNPALEQRYVKATKADVGGVGGHVVAAEEVKSIVAQFVLDNNAYQGR
                     PIYTSLIVTHTGDDVAVTGIIDEHVDQGVVDELMWDALQEGARKAAELGLYGPGQDLV
                     ADAFTGNLRGAGPATVTLPLPVRKDGNASQTVLLSFCDKTEPMAFNYYATGAYLLPRF
                     NTGLVIASSKMKRGYVFEIVDLDTKAQAIEAGVHPRDQKALDGKMEELAKGLQEKVIR
                     LRAPEDLFDIEGLCRSSRFVVARIWSRNEHGEPDQLGYVCSAERLHNIKTKKGFTYGG
                     KDDPVLLAFAQGDWPAPGEITSPWAAAPLVAGDCRGSHYLHILPMPINSQTSYWSGPI
                     ISAVTLSCNIHTGRIGAISDQFALGTPWDEVRRRASDLAIQFRMAHGIKQPATLHEDE
                     LEYQEGWKERRARLERQFEVRPPLTFGGNGNGHGAGGPAGERVAASRGGRPDEID"
     gene            10430..11773
                     /gene="miaB"
                     /locus_tag="VLF19_10635"
     CDS             10430..11773
                     /gene="miaB"
                     /locus_tag="VLF19_10635"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HSE93753.1"
                     /translation="MAKLHLITYGCQMNEYDSERVAGLLREQSWELTESDAEADLILL
                     NTCAIREKCEDKVFSKLGELRRLKASRPDLVVGVMGCMAQLHQADIRRRAPVVDLVFG
                     SPAIARVGDLVARVRAGQRPLVETGEAPLVKITARPAGDRPLRSYVTVMEGCEKHCTF
                     CVVPMTRGRQRSHAPASILAEIRGLAAEGCRDVTLLGQTVEMYGRDLMPATDLAALLA
                     QVNDVPGLARIRFTTSNPFNFTRRLIRAIRDVPKVCEWVHLPLQSGSNRVLERMNRGY
                     TRERYLDLIAELRETVPDVALSTDLIVGFPGETDADFEATLDTVERVAYDNVFVFRYS
                     ARPGTPAARYAEQVPLDTKAHRNARLLETAGRIAVERASALAGRTLEVLVDGRSRKSA
                     GELQGRTRCNRVVNFEAPPGVAVGDVVGVRITEALPHSLRGVVASLPEEAVCLSR"
     gene            11758..12264
                     /locus_tag="VLF19_10640"
     CDS             11758..12264
                     /locus_tag="VLF19_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSE93754.1"
                     /translation="MFVEMKVRGLALDPLSNMPIIILRDEEDKRSLPIWVGIFEANAI
                     ALELEKISTPRPMTHDLIKSILETVEVRVNKVVVTDLKENTFFAVLHLQLGETEYTVD
                     SRPSDAIALALRVAAPIYVDEEVVRKAKTLDVATKEGEPVKADDPDQIKEWLGKIKPE
                     DFEKFNKA"
     gene            12270..13427
                     /locus_tag="VLF19_10645"
     CDS             12270..13427
                     /locus_tag="VLF19_10645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSE93755.1"
                     /translation="MRIALFTNNYLPFCGGVTTSVETLRHGLEGRGHEAWVFAPHMPA
                     ATASPRVVRYLSLPAATYPEFALAIPVSRRITRLVRALDPEVFHAHHPFLLGPAARRL
                     SHRARRPLVFTYHTRYEKYAHYVPLRRGLVEAAAVGLSTRFAGRADAVIAPSAVIRDE
                     LRGRGVKTPIAVVPTGVDLSRFTPGDRYAARRALGLPEDEPLVLYVGRLDREKSVDRV
                     LAAFERVAGTVPRCRLLLVGRGTQADVLARHAASLPIGDRILFLGARPHGALPSCYRA
                     ADLFLFASETETQGLVLAEAAACGLAAVAVAAPGCDEVVRDGETGLLTKAEPAALADA
                     AIGLLLDGERRRGMARRAREVAERDFDVRLQIDRTLAVYAEAAARVAGQRR"
     gene            13443..13766
                     /locus_tag="VLF19_10650"
     CDS             13443..13766
                     /locus_tag="VLF19_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93756.1"
                     /translation="MVRRGEALDRIEKELVAEKAAALGRAGERLERALLEVAALGARY
                     DVAREPADRARLGRDYERARAGAASARLALLIQREAVGLRHHRVVDQQFPEPPSLRAR
                     RAPRA"
     gene            13769..15076
                     /locus_tag="VLF19_10655"
     CDS             13769..15076
                     /locus_tag="VLF19_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HSE93757.1"
                     /translation="MWNPAAECQSRAEREALQLGRLRETLAWAASRVPFYRERLPAEP
                     VRDLGDLARLPFTRKSDLREHYPFGLFAVPQPDVARIHASSGTRGKPTVVGYTAGDLD
                     VWNEVMARVMVAAGARAGDLLHVAFGYGLFTGGLGFHQGAERIGMTVVPASSGNTARH
                     CLLLHDLRPAGLCATPSFALHIAETLADQGVKAGALGLRYGMFGAEPWTQGVRRALEA
                     GFGLRAHDVYGLSEIVGPGVSGECEARDGLHIADDHFLPEIVEPETGRPLPPGRQGEL
                     VLTTLTKRALPMIRYRTGDITTLSLEPCRCGRTSARMARLVGRADDMLVIKGVNVYPS
                     EVEGALLDVGELVPQYQLVVDRRTTLARVEVQVEPAPAFLARCGALDPGLPAVAALRE
                     HVAARLRGALGLAVEVTLVPPRTIPRSEGKAVRVIERTEDARR"
     gene            15073..15894
                     /locus_tag="VLF19_10660"
     CDS             15073..15894
                     /locus_tag="VLF19_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HSE93758.1"
                     /translation="MSDRATVMMAKIQSGARLESAEEMTEEYREHLVHLMTMQADSEL
                     AGGYGYVPWITRAPTVEEKHVVAQIVKDEIRHATVMYGLLADLGFDVDGHVRRHDEIF
                     TMRIDASADIGTQRITSDKRVNIFYYPIETWADFIFFNFCMDRGAGHQLEDVRHCSYG
                     PWARAIEGIFKEEKFHIRHGEHWVKKLAEDLATRDEAQATFDKWYVRTMNIFGRPGSA
                     KNQRYRDLGLKARDNDEVRQAFATEVKGLCDKFGLNIPEWKPKWSDLPEEAQIPG"
     gene            15899..16363
                     /locus_tag="VLF19_10665"
     CDS             15899..16363
                     /locus_tag="VLF19_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:16034..16036,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSE93759.1"
                     /translation="MTNVRFAAPAPDFSLPSTEGREITLAEHRGRDVVLVFYCYDFGT
                     IUTPELGGLAQVAERIAERQAVILAISGDSPFCHKAYNDAWKFPFPLLSDVHRKVISA
                     YGVLDRDRNVAYRSTFIVDRDGVLRWGQAGDRQMVRDGAEILRLLDLIRSLR"
     gene            complement(16570..17199)
                     /locus_tag="VLF19_10670"
     CDS             complement(16570..17199)
                     /locus_tag="VLF19_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93760.1"
                     /translation="MIALPRLSWRLVAVAGGGVVLVAALAVGLWLWSDTQQRRATAAH
                     AAALARLGQTRSREAGEARAAAVRDLEAVLASHPSSPLAAQTALEIGNARYAERDWAR
                     ARGAWAVAAGQARAPTVRTLAREGIGYAWEGERDWARAAEAFQAALADLKPGDFHYEE
                     LLIDLARVQELGGQKVAAIETYRRLLKEVPGSLRADDVRTRLASLGAAP"
     gene            complement(17505..17753)
                     /locus_tag="VLF19_10675"
     CDS             complement(17505..17753)
                     /locus_tag="VLF19_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008557521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93761.1"
                     /translation="MYRLVLAVVVAVAAFLVAAPVQANQCPKLIAQINAAAGNRFDNA
                     SYMAKQKAAQAQKLHAEGKHAESEKIAKEALGQLGVKM"
     gene            complement(17816..18127)
                     /locus_tag="VLF19_10680"
     CDS             complement(17816..18127)
                     /locus_tag="VLF19_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSE93762.1"
                     /translation="MGSRVKIGRAAEVPAGEGCVVEAGGRALALFNVGGRFYAIDNAC
                     AHRGGPLGEGELTGSVVTCPWHAWRWDVTTGANVNNPAVRVACFPVILEGGDVFVDLP
                     R"
     gene            18190..19563
                     /locus_tag="VLF19_10685"
     CDS             18190..19563
                     /locus_tag="VLF19_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE93763.1"
                     /translation="MIFQQVQNEESGCLSYLIGCEQAGQAIVIDPGRDRVDDYVRLAR
                     RKGLRITRIIETHTHADHISGNRDLAAVTQAVIHLHARAGAAFEHETVSDGAELALGN
                     VRLKVLHTPGHTPDSVCLLVTDTSRGGEPWFVLTGDTLFIGDVGRPDLGGAPAAGDLY
                     DSLHATLLALPDSVEVYPAHGAGSLCGRAMSSKAASTIGFERRFNAALQARSRAEFVN
                     GLMAGLPPKPPSFETIVAKNRGLLPLVAAKPRPYTARETWEAMQRGACVLDLRDSGTY
                     GEGHVPGAVNVWIESPQFADRASWFLPAGAPVILLAQGPSDLERALGGLARVGVDQVA
                     GFLQRGMIEWRSEALPVASVPQITVYDLAEWIEERRDVVVVDVREPFEWREGHIDGAL
                     HLPMLEAVGRLAELPSDRPKAVLCAGGLRSSTVISALQRLGVGGWYNVTGGMTAWLKA
                     GHPVTRP"
     gene            19560..>20096
                     /locus_tag="VLF19_10690"
     CDS             19560..>20096
                     /locus_tag="VLF19_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93764.1"
                     /translation="MNRSVRAARRGPRRRAAWALLAVATLLGGCATTYRQGASAYRRG
                     DYATAAERFQAALAADPQRVDALVGLGLARYSTGDWPAATDTLERAVALAPRDATARL
                     YLGLAHLRRGDDAQAVAHLGAFRDLAAGTRTAGQADRALRLIREMPMTPELRVFVAVS
                     LEEASAMQHELAAALEALR"
BASE COUNT         3028 a         7180 c         7018 g         2870 t
ORIGIN      
        1 tcgggcgggg gcgcaaggcg agggtggcct acgggttcga cgacatcgcg ctcgttcccg
       61 gggccgtcac cgtcaatcct ggcgaggtgg acatctcgtg ggagctgtgc ggccgccgct
      121 tcgagctgcc gatcatcgcc gcggccatgg atggtgtggt gagcccgccc ctggccatcg
      181 agatgggacg cctgggcggc ctcgccgtgc tgaacctcga gggtatcttc tcgcgctacg
      241 agaaccccga cgatgtgctc gatcgtatcg tctccgccag ccaggccgag gcgacgaaga
      301 tcatccagtc gatctacggc gagcccatca aggaggagct gatccaccgg cggatcggcg
      361 agatcaagaa gggtggtagc gccgtcgccg tctcctcgat cccgcagcgc gccgagcgct
      421 tcgcccagat cgcccaggag gctggtgccg acgtcttcat cgtgcagtcc accgtgacga
      481 cggcgcggca catcgccacc gaatacgccc cggtggacct gaggcgcctg aagaagcagc
      541 tggcgatccc gctcgtcatc ggtaactgcg tgacctacga ggcatgcctg gagctgatgg
      601 agtgcggcgc cgacgccctg ctgatcggcg tcggtccggg cgcggcgtgc acgagccgcg
      661 aggtgctcgg cctcggcgtg ccgcaggtga cggccacggc ggactctgcc gccgcgcgcg
      721 acttctacta caagcagacc ggccgctacg tgcccatcgt gaccgacgga ggcatgacca
      781 ccggcggcga cgtgtgcaag gcgctggcgt cgggcgccga cgccgtcatg atcggctcgg
      841 ccttcgcccg cgccgtcgag gccccgggcc ggggctacca ctggggcatg gcgaccccgc
      901 accagaacct gccgcgcggc acccggatcc gggtgggcat cgccggctcc ctcgagcaga
      961 tcctcttcgg accggccttc acggaggatg gcacgctcaa cctggtcggc gccatccgca
     1021 cgtgcatggg ctcggtcggc gcgcgcagca tccgcgagct ccagcagacc gagctgatca
     1081 tcgcgccggc catcaagacc gagggcaagg tcttccagca ggcccagaag ctcgggcggc
     1141 cccgctagcc ccgccgtcag cgaggttcgc atggacgcgg ccgacaagat cgcgatcctc
     1201 gatttcggag gacagtacac gcagctcatt gcccggcgca tccgggagat gggcgtgtat
     1261 tccgagatcc tgccgtgcac ccagcccctc gagtccgtcc tcgccggcgg ttaccgcggc
     1321 atcgtgctct ccggcgggcc gtcgagcgtc tacgaggacg cagcgccgct cccgccgaag
     1381 gccctcttcg aggccggtgt gcccgtgctc ggcatctgct acggtatgca ggcgatgggc
     1441 tacctgctgg gcggccacgt cgtccccgcc gagcggcgag agtacgggcc ggccgaggtc
     1501 gccctccagg ggtcgagccc gctctttgcg ggcgtgacgc cggaagcgag cgggcgggtg
     1561 gccgtctgga tgagccacgg cgacaacgtc ctcaagccgc ccaagggctt cgtgtcgctg
     1621 gggacgacgc cgaactgtcc catcgccgcc atggccgacg agcagcggcg gctctacgcc
     1681 gtgcagttcc atccggaggt cgcccacacg ccccagggac ggaggatcat cgagaacttc
     1741 ctcggcgtgt gtcgcgcccg cgcgacctgg tcgatggcat cgttcatcga gacgaccgtg
     1801 gccgagatcc gggcgaaggt cggccaggac cgcgtcctct gcgcgttgag cggcggcgtc
     1861 gactcgtccg tggtcgcggt gctggtccac cgcgccatcg gcgatcagct cacgtgcatg
     1921 ttcgtcgaca acggcctgct caggaagggc gaggcgaatg ccgtcgtgca taccttccgc
     1981 gacgccttca agctcaacct cgtccacgtc gacgcgtcca ggcgattcct cgacgccctg
     2041 cgcggcgtca ccgaacccga gctgaagcgc aagcggatcg gcgcggagtt catcgcggtg
     2101 ttcgaggagg aggcgcgacg gctcggcgag attccgtggc tcgcgcaggg cacgctctac
     2161 cccgacgtga tcgagtcgat ctcgttcaag gggccttcgg cgacgatcaa gacgcaccac
     2221 aacgtcgggg ggctgccgac caagatgcgc ttcaggctcc tcgagcccct ccgggagctc
     2281 ttcaaggacg aggtacgtcg agtcggagag ctcctcgggc ttccctccga gatcgtgtgg
     2341 cgccagccgt tccccggtcc aggtctggcc atccgcgtgc tgggcgaggt gaccgaggag
     2401 cggctcgacc tcctgcgcgc ggccgacgcc gtcgtccagg aggaggtgcg cgcggccggt
     2461 ctggagcgcg agctgtggca ggccttcgcg gtgctgcttc cggtgaagac cgtgggcgtg
     2521 atgggagact tccgcaccta cgcccacgtg gtcgctctgc gggccgtgat gagccaggac
     2581 gcgatgaccg cggactgggc gcggcttccc tatgatgtcc tgggccgtat cagtaaccga
     2641 atcatcaatg aagtcaaggg gatcaaccgg gtcgtcttcg acatctcttc aaaaccaccc
     2701 agcaccatcg aatgggagta gcacgccgcc cccacgtatg actcactccg agttcgtcca
     2761 cctgcacgtg cactcggagt acagtctcct cgacggcgcc gctcagctcg agaagctcgt
     2821 cgccaaggcc aaggagctga agttcccggc gctggcgctg accgaccacg gcaacctgtt
     2881 cggagccgtc gacttctacc tggtcgcgca gaaggccggc atcaagccca tcgtgggctg
     2941 cgagctctac gtcgccccgc ggggccgcaa ggagcgcggt agcgaggacg gcggctacga
     3001 gggcgcgaat cacctgacgg tgctggtccg caaccttgcc ggctaccaga acctgatcaa
     3061 gctcgtctcc cgcgcctacc tcgagggctt ttactacaag ccccgcgtgg acaaggagct
     3121 gctcgccgag cacgccgacg gcctgctcgt gctctcgggc tgcctcaact cggaagtcgc
     3181 ccggctgctc gcggcagggg agatcgaccg ggccagggag gcggcggggt ggtaccagga
     3241 ggtcttcggt aaggaccatt acttcatgga ggtccaggcc cacggtctgg acgagcaggt
     3301 gaaggtgacc gcggccacgc gtgagatcgc cagggcgatc ggcgcgccga tcgccggcac
     3361 caacgactcc cactacctcg aggccggcca cgcgcgcgcc cacgaggcgc tgctgtgcat
     3421 ccagaccggc gcgacgctgc tcgatcccaa gcgctggcgc ttctccagcg aagagttcta
     3481 cgtgaagtcc gccgaggaga tggcgcgggt gttcgccgag attcccgacg cgtgccgcaa
     3541 cacggtggcc gtcgccgagc gctgcaacct cacgctggag ttcggacgct ttcacctgcc
     3601 gcgttacgtc gtgcccgacg gccacacgct cgattcctac ctggagcacc tctcccgcca
     3661 gggactccga cggcgctacg ggccgacgcc cggcgacgcc atcgaggcgc ggttgggcca
     3721 cgagctcagg atcatcgaga agatggggtt cgcgggatac ttcctcgtcg tgtgggactt
     3781 catccactac gcgcgccagc agggcgtggc ggtggggccg gggcgcggat cgtccgccgg
     3841 gtcgctcgtc gcctacgcgc tgggcatcac caacgtcgac cccatccgct acggactcct
     3901 gttcgaacgg ttcctgaacc ccgagcggat ctcgatgccg gacatggaca tcgacttcgc
     3961 cgacgaccgc cgggacgagg tcatccgcta cgtcgccgag aagtacggcc gcgaccgggt
     4021 tgcccacatc atcacgttcg gcacgctcgg agccaaggcc gccatccgcg atgtgggccg
     4081 ggtgctgggc atgccctacg ccgacgtcga ccggatcgcg aagctcgtcc cgaacttccc
     4141 gctgaacatc acgctcgacg acgcgtacca gaaatcgccg ccgctggcgg agatggtcag
     4201 gacccaggcc ggcgtgaagg agctgtggga catcgcccgc gccctcgagg gctgcacccg
     4261 ccacgcctcc gtgcacgcct cggccgtggt catctcagac gatccgctcg acgatcacgt
     4321 gccgctatac aaggacccca agcggcccga gctgatcacc gggtacgcga tggggccgat
     4381 cgagaagctc ggtctcctca agatggactt cctggggctg cgtacgctga cggtgctcgc
     4441 caacaccgtg gccctgatcc gcgaggcgcg ggggctgacg ctcgacctcg acgcgctgcc
     4501 gctcgacgac gccaagacct acgcgctgct gaccgacgcc aagaccttcg gcgtgttcca
     4561 gctggagtcg ggcggcatgc gcgacgccct gcgcgggctc cggccggagc ggctcgagga
     4621 cgtcatcgcg atggtgtcgc tctaccgccc ggggccgatg gagctgatcc ccgaattcat
     4681 caaccgcaag cacggacgcg ccaagatcac gtacgagcac cccgcgatgg agacgttcac
     4741 gcgggagacg tatggcatca tggtctacca ggagcagatc atgcagatcg cctccgagat
     4801 ggccggcttc accatgggcg aggcggacac gctccgccgc gccatgggga agaaggaccg
     4861 cgatctcatg gcccagcagc gcgacaagtt cctggccggc tgcgccgagc gcgggacgcg
     4921 gcgcctgcag gccgagcgcg tctgggagct catggagaag ttcgcggggt acggcttcaa
     4981 caagtgcctc actgcggaca cgtggatcga gatggccgac ggctcgcgca agccgatcac
     5041 ggcgatccgc gacggcgacc tggtgctcac caaggacggc ccgcacgccg cgcgccggat
     5101 ccgcccgagc ggcatccggc cggtggggcg gctcgtcctg gccaacggca tgaccgtgcg
     5161 ctgcacgccg gatcatccga tcttcaccca gcgcggatgg gtcgacgcgc aagagctcgc
     5221 cggcggcgac gccgtggccg tgctcgacac cgtcgccgcc gccgatctcc gcgtggccgc
     5281 gcggcgcgcc gcgccgggtc gcgcctccgt gatcaccgag acgccgatcg actggtcggc
     5341 gcccgcgtcc ttcacccccg acggcgtgga gccgacctac gacttcgagg ttcccggtgc
     5401 ggggagcttc atcgccaacg gcatcgtcgt ccacaactcc cacgccgccg cctacgcgct
     5461 cgtcgcctac cagacggcct acttcaaggc gaactacccc gtggagttca tggccgcact
     5521 cctcacatcg gaaatgggcg actccgacaa gatcgtgaag tacatcgagg agtgccgcgc
     5581 gatgggcatc cgcgtggagc cccccgatgt caacgtctcc gcggtgaagt tcagcgtgga
     5641 gggcgccacg atccggttcg ggctggcggc gatcaagaac gtcggcgagg cggcgatgga
     5701 gtcgatcctc gcgacccggg accgggaggg gcggttccag gggctggagg acttctgcgc
     5761 gcgggtcgac ctgcggctgg tgaaccggcg cgtcctggag agcctggtca aggccggcgc
     5821 cttcgactcg ctggggctca cgcgcgcgca cctgctggcc acggtcgacg ccgcgctcga
     5881 gagcggccag cgccagcagc gcgagcgcgc cgagggccag gggtcgttct tcgacctgct
     5941 ccccggcgtg gccgcgacgc cgaggaccga gccagtggcg gtgacgccgg agtgggacga
     6001 cgatcagcgc ctgcagttcg agaaggaagt gctcggcttc tacgtctcgg gccacccgct
     6061 ggcgcgctac ggccccctcg tggagtcgct cggcatcacg acgaccgacg acctggcggg
     6121 caagagcgcg ggcagccgcg tgctcctgct cgggcaggtg gcggcgctca aggagacggc
     6181 gacgaagagc ggcaaccgca tggccttcgt cacgctggag gacatggccg gcaccgtcga
     6241 ggtcaccgtg ttccccgagc cgttcaagtc cgccgccgag cacctgcggg cccgcgagcc
     6301 ggtgctggtg cgcgggcgcg tggacgacag cgagcgcggt cgttcggtgc tggccgagga
     6361 cgtccggctg ctcgagcgcg tcctcaccga cagcggcgcc cgggcggcga ccacgcccgg
     6421 cgagccgaac gccgtgcgga tccgggtcgc cgcgacggag gatcccaggg ccgtgctggg
     6481 ttcggtccgc gaggcctgcg ccgagcatcc cgggccggtg cccgtcttcg tgcacgtcct
     6541 gctgcccggc cgcgaggtcg tggtgcgctc acgggcgctc gccgtcgatc cagcgccgcc
     6601 gctcctcgag cggctcgcgg cgttgctcgg cccgacggcg acggtggtgg agcatgccgg
     6661 aggcgcttga gttcgaacag ccgctgctgg agctggaggc gcggatcacg gccctgcagg
     6721 ccctcgacga ccccgcccag cgcgacgaga tcgggcgcct cgaagaccgc ctggcgcgcc
     6781 tgcgccagaa gaccttcgcc ggtctcaccg cgtggcagac gacccagctc gcgcgccacc
     6841 cccggcggcc gcacatgcgc gacttctgtc gcctggtctt cgaggacttc atcgagctgc
     6901 acggcgaccg cctctacgcc gacgaccagg cgatcgtcgg cggcctggcg tgcttcgagg
     6961 ggtacggcgt cgtcgtcatc ggccaccaga aggggcggga gacgcgcgag aagctcgcgc
     7021 ggaacttcgg gatgccgcac ccggagggct accgcaaggc gctgcgcctg atgcggctcg
     7081 ccgagaagtt cggcaagccc gtcgtctcgc tcatcgacac gccgggcgcc tatcccggca
     7141 tcggtgccga ggaacgcggc caggccgagg ccatcgcgcg caacctgcgc gagatggcgg
     7201 cgctgcgcac gcccatcgtc gccgtggtca ccggggaggg tggaagcggc ggcgcgctgg
     7261 cgctcggcat gggcaaccgg gtgctcatgc tccagtacgc cgtatattcc gtcatctcac
     7321 cggagggctg cgcggcgatc ctctgggggg acgcggcgaa ggcccccgac gccgccgaga
     7381 tcatgaagat cacggcgccc gacctgctgc gactcggcgt catcgacggc atcgtccccg
     7441 agcctgccgg cggcgcccac cgcgactggg atgtgaccgc cgccaacctc aaggcggcgc
     7501 tggccacgca cctggcggac ctgaaggtca agagcccgga ggccctggtg acggagcgct
     7561 acgacaagtt ccgccgcatc ggcgccttcg aggaggaggc cgttcccggg cgccggtagg
     7621 ccggctaggg cttgagggcg gcctgggcca cgtcctcgtc ggtcacgagg acgttcatga
     7681 accggccgcg aagtgctccg cggacggcgt ccgtcttcga gcgaccgccg gccaccgcca
     7741 cgacccagcg gtcctcccgc cgggacagct cctggaggcg ctcgccctcg accgacagca
     7801 cccggtgcat cagcgcgcgc agctccggct cggcgacgat gcgcccctcc gcgtccacgg
     7861 gctggtagtt gatctcgccg accacgccga actcccgcaa ccgcttcacg ctgaccccgt
     7921 aggactcggc cagcgagcag aagcccgggg tctgctcggc gatctggccg acgccgacga
     7981 gcgcgatgtc taccgaccgc gccgtctcgt agatcctccg gacctcgggg tcgcgcagca
     8041 ggcgccgccg ctcgcgctcg atctgccgca gcgagatgag gtgctggacg ggcagggcgt
     8101 aggcggtcac gtggggccgg tacttcgcgg ccatcatgcc gacgagggtg ttcggcgaga
     8161 tgtccacgag cttcagcgtg ctctcgcccg acaacggata gagggcgagg tcccgtaggc
     8221 gccgctcgcg cagcgcgcgc acggtgtggt agagcgtgaa gccgcacgag atgcccacgc
     8281 gcatgccgct ggtcacggtc ttctcgaagt agcccgccgc ggcgacgccc agctcctcgc
     8341 gcacgtcgcc gcgtccgccc gcggcgacca ccaccgcgtc ccgcaggccg aagtgctgca
     8401 tcagcgcgac ttccagctcc tgtgtccgcg gcaggtccag ttccacgcgc acgaggccct
     8461 cgtcgaaggc ccgcttgagc aggcgggaga cggtggccgc cgagaccccc agcgcccggg
     8521 cgatgtcctt ctggctgcgt ccctggcggt agtacagctc gaggcactgg accatctgcc
     8581 gagactcgcc ggggcggtga gaaattaatt tcaccatgct caagttcttg cttgacggcg
     8641 ccagtctacc gccctatcct tcagccgtca ccggtttctt ccacgccaac cggaccggat
     8701 gcgacgcagg aggaggggga tcgcatggca ggcaagcggt acgggtcggg caacggcgcc
     8761 cgcggccccg cgctgggcga ctatcatcag tccaaggacg agctggccgg ccttcctcag
     8821 acggtggccg agaagatccc ggtcaccaac tcgccaaaca tcttctcgta cagggcgtac
     8881 gtcttcgcga agaaccccgc gctggagcag cgctacgtca aggccaccaa ggccgacgtc
     8941 ggtggtgtcg gcgggcacgt ggtcgccgcc gaggaagtga agtcgatcgt ggcgcagttc
     9001 gtcctcgaca acaatgcgta ccagggccgg ccgatctaca cctcgctcat cgtcacccac
     9061 accggggacg acgtcgccgt caccggcatc atcgacgagc acgtcgacca gggcgtggtg
     9121 gacgagctca tgtgggacgc cctgcaggag ggcgcgcgca aggccgccga gctcgggctc
     9181 tacggaccgg gccaggacct ggtggccgac gcgttcaccg gcaacctccg cggcgccggg
     9241 ccggcgacgg tcacgctgcc gctgcccgtt cgcaaggacg gcaacgcctc gcagacggtg
     9301 ctgctgtcct tctgcgacaa gaccgagccg atggccttca actactacgc caccggcgcg
     9361 tacctgctgc cgcggttcaa cacgggcctg gtgatcgcgt cgtcgaagat gaagcgcggc
     9421 tacgtgttcg agatcgtcga cctcgacacc aaggcccagg ccatcgaagc cggcgtccat
     9481 ccccgcgatc agaaggcgct cgacggcaag atggaagagc tcgccaaggg gctccaggaa
     9541 aaggtcatcc gcctccgcgc gccggaggac ctgttcgaca tcgaggggct ctgccgctcg
     9601 tcgcgcttcg tggtcgcccg catctggagc cgcaacgagc acggcgagcc cgaccagctc
     9661 ggctacgtct gctcggcaga gcggctgcac aatatcaaga ccaagaaggg gttcacgtac
     9721 ggcggcaagg acgaccccgt gctgctcgcc ttcgcccagg gcgactggcc ggcgcccggc
     9781 gagatcacct cgccgtgggc ggccgcgccg ctggtcgccg gcgactgtcg gggcagccac
     9841 tacctgcaca tcctcccgat gccgatcaac tcccagacgt cgtactggtc ggggccgatc
     9901 atctcggccg tcacgctctc ctgcaacatc cacacgggtc ggatcggcgc gatctccgat
     9961 cagttcgcgc tcggcacgcc gtgggacgag gtgcgccgcc gcgcctccga cctcgctatc
    10021 cagttccgca tggcccacgg catcaagcag cccgcaacgc tccacgagga cgagctcgag
    10081 taccaggaag gctggaagga gcgccgcgcg cgcctcgagc gccagttcga ggtgaggccg
    10141 ccgctgacgt tcggcggcaa cggcaacggc cacggggccg gtgggccggc gggcgagcgc
    10201 gtggctgcga gccggggcgg gcgaccggac gagatcgatt gacggcacgc gagggggcga
    10261 gacgccccct cgcgaagctt tccggcccgc cgccgacccc tcgcacagtg cgtgacggcg
    10321 ggagacatgt ccaagagggc tcgccgagag ggcgcggaac cccgcgccga gtttgacttc
    10381 ccccggggtg ggtgctacgg tcggactcaa cgttggcgcc gatttcccaa tggccaagct
    10441 ccacctgatc acgtacggct gtcagatgaa cgagtacgac tcggagcgcg tcgccggcct
    10501 gctccgcgag caatcgtggg agctgaccga gagcgacgcc gaggcggacc tcatcctcct
    10561 caacacgtgc gccatccggg agaagtgcga ggacaaggtc ttctcgaagc tcggcgagct
    10621 gcggcggctg aaggccagcc gtccggacct tgtcgtcggc gtgatgggct gcatggctca
    10681 gctccaccag gcggacatcc ggcgccgcgc ccccgttgtc gacctcgtgt tcggctcgcc
    10741 ggccatcgcg cgcgtcgggg acctcgtggc gcgggtccgc gccggccagc gtccgctcgt
    10801 cgagaccggc gaggcgccgc tggtgaagat caccgccagg cccgcgggcg accggccgct
    10861 ccgctcgtac gtcaccgtga tggagggctg cgagaagcac tgcacgttct gtgtggtgcc
    10921 catgactcgg ggtcgccagc gtagtcacgc gcccgcctcc atcctcgccg agatccgtgg
    10981 gctcgccgcc gaaggttgcc gcgacgtcac cctgctcggg cagaccgtgg agatgtacgg
    11041 acgagacctg atgccggcga cggacctggc cgcgctcctc gcccaggtga acgacgtccc
    11101 cgggctggcg cgcatccggt tcacgacctc gaacccgttc aacttcacga ggcgcctgat
    11161 ccgcgccatc cgggacgtcc cgaaggtctg cgagtgggtc cacctgccgc tgcagtcggg
    11221 atccaaccgg gttctcgagc ggatgaatcg cggctacacg cgcgagcggt atctcgacct
    11281 gatcgccgag ctgcgcgaga ccgtgccgga cgtggccctc tccaccgatc tcatcgtcgg
    11341 cttcccgggt gagaccgacg cggacttcga ggccaccctc gacacggtgg agcgcgtcgc
    11401 ctacgacaac gtcttcgtct tccggtactc ggcccggccg ggcacgccgg ccgcgcgcta
    11461 cgcggagcag gtcccgctcg acacgaaggc ccatcgcaat gcgcgcctcc tcgagacggc
    11521 cgggcgcatc gccgtcgagc gggcgtccgc gctggccggg cgcacgctcg aggtgctggt
    11581 ggacggacgg tcgaggaaga gcgccggcga gctgcagggc cggacgcgct gcaaccgcgt
    11641 cgtgaacttc gaggcaccgc ccggcgtggc cgtcggtgac gtcgtcggcg tccggatcac
    11701 cgaggcgctg cctcacagcc tgcgcggcgt cgtcgcgtcg ctccccgagg aggccgtatg
    11761 tttgtcgaga tgaaggtgcg cgggctggcg ctcgatccgc tgtccaatat gccgatcatc
    11821 atcctccggg acgaggagga caagcggtcg ctgcccatct gggtcggcat cttcgaggcg
    11881 aacgcgatcg cgctcgagct ggagaagatc tccaccccgc ggccgatgac gcacgatctc
    11941 atcaagagca tcctcgagac cgtcgaggtc cgggtgaaca aggtcgtcgt caccgacctc
    12001 aaggagaaca ccttcttcgc cgtgctgcac ctccagctcg gcgagacgga gtacaccgtc
    12061 gactcccggc cctccgacgc gatcgcgctg gcgctccggg tggccgcccc catctacgtc
    12121 gacgaggaag tcgtccggaa ggccaagacg ctcgacgtcg ccaccaagga gggcgagccc
    12181 gtgaaggcgg acgatcccga ccagatcaag gagtggctgg gcaagatcaa gccggaggac
    12241 ttcgagaagt tcaacaaggc ctagcctccg tgcgcatcgc gctcttcacg aacaactacc
    12301 tgccgttttg cggcggcgtc accacgtcgg tcgagacgct ccggcacggg ctcgagggcc
    12361 ggggtcacga ggcctgggtg ttcgcgccgc acatgccggc ggcgaccgcg agcccccgcg
    12421 tggtgcggta cctctcgctg cctgccgcga cctatcccga gttcgcgctg gccatccccg
    12481 tgtcacgccg catcacgcgc ctggtgcgcg cgctcgatcc cgaggtcttt cacgctcacc
    12541 atcccttcct gctcggaccc gcggcgcgcc ggctgtcgca ccgcgcgcgc cggccgctgg
    12601 tcttcacgta ccacacccgc tacgagaagt acgcgcacta cgtgccgctc cggcgcggcc
    12661 tcgtggaagc ggcggccgtg gggctgagca cgcgcttcgc gggccgcgcc gatgccgtga
    12721 tcgcgccgtc ggcggtgatc cgcgacgagc tgcgcgggcg cggcgtgaag acgccgatcg
    12781 cggtcgttcc cacgggcgtg gacctctccc ggttcacgcc cggcgaccgc tacgcggcgc
    12841 gccgggcgct gggcctcccc gaggacgagc ccctggtgct gtacgtgggg cgcctcgacc
    12901 gcgagaagag cgtggaccgt gtcctcgccg ccttcgagcg cgtggccggg acggtgcctc
    12961 gctgccggct gctcctggtg gggcgtggga cccaggccga cgtgctcgcg cgccacgcgg
    13021 cgtcgctccc catcggcgac cgtatcctgt tcctgggcgc gcggccgcac ggcgcgctgc
    13081 cttcctgcta ccgcgccgcc gatctcttcc tcttcgcgtc ggagacggag acgcaggggc
    13141 tggtcctggc cgaggcggcg gcctgcgggc tggcggccgt cgccgtcgcc gcgccgggct
    13201 gtgacgaggt ggtgcgcgac ggcgagaccg gactgctcac caaggccgag cccgcggcac
    13261 tcgcggatgc cgcgatcggg ctcctgctcg acggcgagcg tcggcggggc atggcacgcc
    13321 gcgcgcgaga ggtggccgag cgcgacttcg acgtccggct gcagatcgac cgcacgctgg
    13381 ccgtgtacgc cgaggcggcc gcgcgcgtcg cgggccagcg gcgctgagcg gcgtccacgc
    13441 tcgtggtgag gcgcggagag gctctcgaca ggatcgagaa ggagctcgtg gcggagaagg
    13501 cggcggcgct gggccgtgct ggcgagcgcc tcgagcgggc gctcctcgag gtggccgccc
    13561 tcggcgcccg ctacgacgtc gcgcgtgagc cggcggaccg tgcccgcctg ggccgggact
    13621 acgagcgggc gcgggccggc gcggcgagcg cccggctcgc gctcttgatc cagcgcgaag
    13681 ccgtaggcct gcgccatcat cgggtcgtgg accagcagtt tccggagccg ccgtcactgc
    13741 gggcgagacg agcaccgaga gcgtgaccat ctggaatccg gccgccgagt gccagtcgcg
    13801 cgccgagcgc gaggcactcc agctcgggcg gctgcgggag acgctggcct gggcggcgtc
    13861 ccgcgtgccc ttctatcggg agcggctgcc cgcggagccc gtccgggacc tcggcgacct
    13921 cgcgcggctg cccttcacgc gcaagagcga cctgcgcgag cactacccct tcgggctctt
    13981 cgccgtgccg cagcccgacg tcgcgcgcat tcacgcctcc tcgggcaccc ggggcaagcc
    14041 gaccgtggtg ggctacaccg ccggcgacct cgacgtctgg aacgaggtca tggcgcgcgt
    14101 gatggtcgcg gcgggtgcgc gggccggcga tctcctgcac gtggccttcg gctacggcct
    14161 gttcaccggc ggtctcggct tccatcaagg tgccgagcgc atcggcatga cggtggttcc
    14221 cgcctcgtcc ggcaacaccg cgcgccactg cctgctgctc cacgacctcc gccccgcggg
    14281 gctctgcgcc acaccgtcgt tcgcgttgca catcgccgag acgctggcgg atcagggcgt
    14341 gaaggccgga gcactcgggc tgcgctacgg catgttcggc gcggagccct ggacccaggg
    14401 cgtgcgacgg gcgctggagg ccggcttcgg cctgcgggcc cacgacgtct acgggctgag
    14461 cgagatcgtc ggcccgggcg tgagcgggga gtgcgaggcg cgcgacggcc tgcacatcgc
    14521 cgacgaccac ttcctgccgg agatcgtcga gcccgagaca gggcgcccgc tgccgcctgg
    14581 gcgtcagggc gagctggtgc tcacgaccct gaccaagcgc gcgctgccga tgatccgcta
    14641 ccggacgggt gacatcacga ccctgtcgct cgagccttgc cgctgcgggc gcacctcggc
    14701 gcgcatggcg cggctcgtcg ggcgcgccga cgatatgctt gtcatcaagg gcgtcaacgt
    14761 gtacccctcg gaggtggagg gcgccctcct ggacgtcggc gagctggtgc cgcagtacca
    14821 gctggtcgtc gatcgacgca cgacgctggc ccgcgtcgaa gtccaggtag agccggcgcc
    14881 cgcgttcctg gcgcgctgcg gcgcgctgga ccccggactg ccagcggtgg ccgcgctgcg
    14941 cgagcacgtc gccgcacggc tgcgcggggc gctgggcctc gccgtggagg tcacgctggt
    15001 gccgccgcgg acgatcccgc ggtccgaagg caaggccgtg cgggtcatcg agcgaacgga
    15061 ggatgcaagg cgatgagcga tcgggcgacc gtgatgatgg cgaagatcca gagcggcgcg
    15121 cggctggagt cggcggagga gatgaccgag gagtaccgtg agcacctcgt gcacctcatg
    15181 accatgcagg ccgactccga gctggccggc ggctacggct acgtgccctg gatcaccagg
    15241 gccccgacgg tggaggagaa gcacgtcgtc gcccagatcg tgaaggacga gattcgccac
    15301 gcgacggtca tgtacggcct gctcgccgac ctcggattcg acgtggacgg ccacgtgcgg
    15361 cgtcacgacg agatcttcac catgcggatc gacgcgtccg ccgacatcgg cacccagcgc
    15421 atcacgtccg acaagcgtgt caacatcttc tactacccga tcgagacgtg ggccgacttc
    15481 atcttcttca acttctgcat ggaccgcggg gccggccacc agctcgagga cgtgcgccac
    15541 tgctcgtacg gtccgtgggc acgcgccatc gaggggatct tcaaggagga gaagttccac
    15601 atccgccacg gcgagcactg ggtgaagaag ctggccgagg acctggccac ccgcgacgag
    15661 gcccaggcca ccttcgacaa gtggtacgtc aggaccatga acatcttcgg ccgcccgggg
    15721 tcggccaaga accagcgtta ccgtgacctc gggctcaagg ctcgcgacaa cgacgaggtc
    15781 cggcaggcgt tcgccaccga ggtgaaaggg ctgtgtgaca aattcgggct caacataccg
    15841 gaatggaagc cgaaatggtc ggaccttccg gaagaagccc agatcccagg ataggcccat
    15901 gaccaacgtc cggttcgccg cacccgctcc cgacttcagc ctgccctcca ccgagggccg
    15961 tgagatcacg ctcgccgagc accggggccg tgacgtcgtc ctggtgttct attgttacga
    16021 cttcgggacc atctgaacac ccgagttggg cggcctggct caggtcgccg agcgcatcgc
    16081 cgagcgccag gccgtgatcc tggcgatctc gggcgacagc ccgttctgcc acaaggccta
    16141 caacgacgcg tggaagttcc cgttcccgct gctgagcgac gtgcaccgca aggtcatcag
    16201 cgcgtacggc gtgctcgaca gggaccgcaa cgtcgcctat cggtcgacct tcatcgtcga
    16261 tcgcgatggc gtgctgcgct ggggccaggc cggcgaccgc cagatggtgc gggacggcgc
    16321 ggagatcctg cgcctgctcg acctgatccg atcgctccgc tgaggacagg gggggcgcgc
    16381 aaaacggccc ccacggatcc gaaccgagcc gcgcgtgggc cgtcgagcgc gcagtcgccg
    16441 gcgagaccgt ccggcgcggc gggccacggg ctgctaggtg cgccgcgtaa gttgacataa
    16501 tgcgccttat cggcgcacgc gcgccgaggc cggcggctcg cgcacggatt tgcgggacag
    16561 cctgcgcggt cagggcgcgg cgccgagact cgccagccgt gtgcggacat cgtcggcgcg
    16621 caggctgccc gggacctctt tgaggagtcg ccggtaggtc tcgatggccg ccaccttctg
    16681 gccgccgagc tcctgcacac gcgcgaggtc gatcagcagc tcctcgtagt ggaagtcgcc
    16741 cggcttgaga tcggcgaggg cggcctggaa cgcctcggca gccctggccc agtctcgctc
    16801 cccctcccag gcgtagccga tgccctcgcg cgccagcgtc cggacggtcg gggcccgcgc
    16861 ctgaccagcg gccaccgccc aggcaccgcg cgctcgcgcc cagtcgcgct ctgcataccg
    16921 ggcgttgccg atctcgagcg cggtctgcgc cgccagcggc gaggacgggt gcgaagcgag
    16981 cacggcctcg aggtcgcgca cggccgccgc acgggcctcg ccagcctccc ggctgcgggt
    17041 ctggccgaga cgcgccagcg ccgcggcgtg cgccgccgtc gcgcggcgct gctgtgtgtc
    17101 cgaccagagc cacaggccga cggcgagcgc cgcgaccagc acgacgcctc cgcccgcgac
    17161 ggcgacgagc cgccaggaaa gccggggtag agcgatcatc ggatcctcac gccttgccgg
    17221 tattgccggc cgcacgatcg acaggccagc catccccgcg cgacccgtcc cacgattctc
    17281 ccgcagtgcg ggcggcgctg gtagcgctgc agggccgaag cagagaccga ccacgcgacc
    17341 gctgcgatgc ccaggaccag cagagggacg gaggggacgg tgtccacgac gagctcctgc
    17401 cagcagcgcg tccccgagag ccgcgagccg atgtctcccc gaacacgccg gggctgtccg
    17461 gacgccgtgc gcacggccgc gacgcccgga cgctccagcc gatgctacat cttgacgccg
    17521 agctgcccga gcgcctcctt cgcgatcttc tccgactcgg cgtgcttgcc ctcggcgtgc
    17581 agcttctgcg cctgggcggc cttctgcttg gccatgtagc tcgcgttgtc gaaacggttc
    17641 ccggcggcgg cgttgatctg cgcgatcagc ttcgggcact ggttcgcctg gaccggagcg
    17701 gccacgagga acgcggcaac ggcgacgacg acggcgagga cgagacggta catgcggtca
    17761 cctccctggg ggtctgggat caccagctgg aaaccgccgg cattataccc gggtctcacc
    17821 gcggcaggtc cacgaagaca tcgccgccct cgaggatcac cggaaagcag gcgaccctca
    17881 cggccgggtt gttgacgttc gcgccggtcg tcacgtccca gcgccacgcg tgccacggac
    17941 aggtcaccac cgaccccgtc agctcgcctt cgccgagcgg cccgccgcgg tgtgcacaag
    18001 cgttgtcgat ggcgtagaag cgaccgccca cgttgaagag agccagcgcc cggccgccgg
    18061 cctcgacgac acacccctcg ccggccggca cctccgccgc gcggccgatc ttgactctgg
    18121 atcccacgat gccgcctccc ggtgtcgttc ggaactggcc cttataatat ccgcggaggg
    18181 gctcgccgaa tgatattcca gcaggtccag aacgaggagt ccggctgtct ttcatatctc
    18241 atcgggtgcg agcaggccgg gcaggcgatc gtgatcgatc ccggtcgcga ccgcgtggac
    18301 gactacgttc ggctggcacg acgcaagggc ctgcggatca cgcgcatcat cgagacccac
    18361 acgcatgccg accacatctc gggcaaccgc gatctcgcgg cggtcaccca ggcggtgata
    18421 cacctgcacg cgagggccgg tgcggccttc gagcacgaga cggtgagcga cggcgccgag
    18481 ctggcgctcg gcaacgtgcg gctcaaggtc ctccacacgc caggccacac ccccgacagc
    18541 gtctgcctgc tcgtgaccga caccagccgc ggcggcgagc cgtggttcgt cctgaccggc
    18601 gacaccctgt tcatcggcga cgtcggccgg ccggacctcg gcggggcgcc ggcggccggc
    18661 gacctctacg acagcctgca cgcgacgctg ctcgccctgc ccgacagcgt ggaggtctac
    18721 ccggcgcatg gcgccggctc cctctgcggg cgcgcgatgt cctccaaagc ggcctccacc
    18781 atcggcttcg agcgccggtt caacgccgcg ctgcaggcgc gcagccgggc cgagttcgtg
    18841 aacggcctga tggccgggct accaccgaag ccgccgtcat tcgagaccat cgtcgccaag
    18901 aaccgcggtc tcctgccgct cgtggccgcc aagccccgcc cctacaccgc gcgcgagacg
    18961 tgggaagcca tgcagcgcgg cgcctgcgtc ctcgacctgc gcgactcggg gacgtacggc
    19021 gaggggcatg tgccgggcgc cgtgaacgtg tggatcgaga gcccccagtt cgccgaccgc
    19081 gcgagctggt tcctgccggc cggcgccccg gtgatcctgc tcgcccaggg gccgtccgac
    19141 ctcgagcgcg ccctcggggg cttggcgcgc gttggtgtcg accaggtggc cggtttcctg
    19201 cagcgcggga tgatcgagtg gcgcagcgag gcattgcccg tggcgtccgt acctcagatc
    19261 accgtgtacg acctggccga gtggatcgag gagcggcgcg acgtcgtcgt cgtggacgtg
    19321 cgcgagccat tcgagtggcg cgagggccac atcgacggtg ccctgcacct gccgatgctc
    19381 gaggccgtcg gccgcctcgc ggagctgccg agcgaccggc ccaaggcggt cctgtgcgcg
    19441 ggcgggctca ggtcgagcac cgtcatcagc gcgctccagc gcctcggggt cgggggctgg
    19501 tacaacgtca cgggcggcat gacggcgtgg ctcaaggccg gtcacccggt gacgcggccg
    19561 tgaacaggag cgtccgggcg gcgcggcgcg ggccccgccg ccgcgccgcc tgggcactgc
    19621 ttgccgtcgc gacgctgctc ggcggctgcg ccaccacgta caggcagggc gcctccgcct
    19681 accggcgcgg cgactacgcg acggctgccg agcgctttca ggcggcgctc gccgccgacc
    19741 cgcagcgcgt agatgccctc gtcggcctcg gcctcgcccg ctactcgacc ggcgactggc
    19801 ccgccgccac cgacacgctg gagcgcgcgg tcgctctggc gccccgcgac gcgaccgccc
    19861 gactctatct cggcctcgcc cacctccgcc gcggcgacga tgcgcaggcc gtcgcgcacc
    19921 tcggcgcctt ccgtgacctg gcggccggca cgcgcaccgc cggccaggcc gaccgtgcgc
    19981 tgcgcctcat ccgcgagatg ccgatgacgc cggagctgag ggtcttcgtg gcggtgagcc
    20041 tcgaggaggc gtcggcgatg cagcacgagc tggccgccgc gctcgaggcg ctacgt
//
LOCUS       DATIAZ010000306         3107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1271575, whole genome shotgun sequence.
ACCESSION   DATIAZ010000306 DATIAZ010000000
VERSION     DATIAZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271575"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VLF19_10695"
     CDS             <1..159
                     /locus_tag="VLF19_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HSE93765.1"
                     /translation="TTAACGTLGGAAVGAGTGAAIGAGTGYGAGKGALIGTGVGAAAG
                     TIYDATRK"
     gene            complement(270..914)
                     /locus_tag="VLF19_10700"
     CDS             complement(270..914)
                     /locus_tag="VLF19_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HSE93766.1"
                     /translation="MKRIAVTLVAATLVAGCSTPLTTREKGALAGGAIGAGTGAIVGH
                     QVGHAGAGALIGAGVGALSGVLIGDAIQGAEQQPAASPAVVAPPQPAPPPAAVTPPPP
                     QVVVMTPPPPVVAPQPVVVVAPPPVAVNHAPRMIWVPEWNAYVLESHDIVYHHGLYYY
                     FHGGRWWAGRSYSGPWVIASSLPPAVARLPRGHLHTHIPKGRHCPPGHARKGHC"
     gene            complement(911..1348)
                     /locus_tag="VLF19_10705"
     CDS             complement(911..1348)
                     /locus_tag="VLF19_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93767.1"
                     /translation="MMGEKSFLMLSLAAALMAPAPAHADVSIGINIGVPEPPPLVVTT
                     PQLVVVPGSTVYYAPGAAYNLFVFGGRYYSFHDGVWFYSATGKGKWTVVPNERVPRPV
                     LAVPTTYYRIPPGHAKRMVRDGAGPEPGRGAGHPGKGRKGHDR"
     gene            complement(1508..2188)
                     /locus_tag="VLF19_10710"
     CDS             complement(1508..2188)
                     /locus_tag="VLF19_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93768.1"
                     /translation="MLDTSADAVLRALNRAYMRRPVLAMVLAAVFGLAVIEGLAFGAP
                     AGATLLFGSLAVLAVVVVSRREIEYYSEAVEYVLDDHATVAYRSLVTAFSRLKTSGPI
                     WHLGRRTTDGQRRHRRLVVPVLALPPRVRSNIRVPALRAGRQTLYFFPDRILVYDTQM
                     AWGIEYRDLKVKGGDVREVTEIGAGGDWAECNGFLALMSRSGLSALFRCADVKAAAEV
                     ASALEGLA"
     gene            complement(2631..2762)
                     /locus_tag="VLF19_10715"
     CDS             complement(2631..2762)
                     /locus_tag="VLF19_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93769.1"
                     /translation="MSRRTVAAAVIWLLTLFFAAQMFAAPVSQLLQRLALTTEASPP"
     gene            complement(2759..>3107)
                     /locus_tag="VLF19_10720"
     CDS             complement(2759..>3107)
                     /locus_tag="VLF19_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HSE93770.1"
                     /translation="ELPYRVVERCVGDLGFSAAKGYDIEVWLPGQGRYREISSCSNYD
                     AFGGRRADIRYKPESGGKPEFCATLNGSGLAVGRTLVAVLENYQEADGTVTIPPVLRP
                     YAGGLERLTPPTR"
BASE COUNT          454 a         1106 c         1120 g          427 t
ORIGIN      
        1 accacggccg cgtgtggcac gctcggtggt gccgccgtcg gcgccggcac cggcgcggcg
       61 atcggcgcag gaaccgggta cggtgctggc aagggggcgc tcatcggcac gggtgtcggc
      121 gcggctgccg gcaccatcta cgacgcgacg aggaagtagc gcgccgccaa cgtcgcctgg
      181 cccccgggaa cactacgggc gaggcggctc atggagcccc tcgcccgttc gccgcgcggg
      241 ttcacggttc gcggaccgcg tttaacccgc tagcagtggc ccttcctcgc gtgacccggc
      301 gggcagtgac ggcccttcgg gatgtgcgtg tgcaggtgac ctcgcggcag cctggcgacg
      361 gcagggggaa gcgatgaggc gatcacccac ggccccgaat acgaccgccc cgcccaccag
      421 cgcccgccgt ggaagtagta gtacaagccg tggtgataca cgatgtcgtg gctctcgagt
      481 acgtacgcgt tccactcggg cacccagatc atccgcggcg cgtgattcac cgcaacgggc
      541 ggcggcgcga ccacgacaac cggctggggc gcgacgaccg gcggaggcgg cgtcatcaca
      601 accacttgcg gtggcggcgg cgtcacggcc gccgggggcg gcgcgggctg gggcggggcg
      661 acgaccgcgg gggaagccgc cggctgctgc tcggcgccct ggatcgcgtc gccgatgagc
      721 accccactca gcgcgccgac gccggcgccg atgagggctc cggcacccgc gtgtcccacc
      781 tggtggccaa cgatcgcgcc ggtgccggct ccgatggcgc cgccggccag cgcgcccttc
      841 tcgcgcgtcg tcaacggcgt cgagcatccc gcgacgagcg tcgccgcgac gagcgtgacc
      901 gcgatgcgct tcatcggtcg tgacccttcc ggcccttgcc gggatggccc gcgcccctgc
      961 ccggttccgg ccctgcgccg tccctcacca tcctcttggc gtggccgggg ggaatcctgt
     1021 agtacgtcgt cgggaccgcg agcacggggc gcggcacgcg ctcgttcggc acgaccgtcc
     1081 acttgccctt gccggtggcg gagtagaacc acaccccgtc gtggaagctg tagtagcgcc
     1141 caccgaacac gaacaggttg taggccgcgc cgggcgcgta atagacggtc gagcccggca
     1201 ccacgacgag ttgcggcgtc gtcaccacga gcggcggcgg ctcgggcact ccgatgttga
     1261 tgccgatgct gacgtcggcg tgggccggcg ccggcgccat cagcgcggcg gccagcgaca
     1321 gcatcaggaa gctcttctcg cccatcatga tgcgatcctc ctaatgtccg gcttccgccg
     1381 cgaagagtgc atcgggagtg ccagacctct cgtccgattt gaacgccact taggccgcgt
     1441 ctccggcacc cggcgggact gggtggttgc cggagtggcg cggaatgtca ggctgtcgat
     1501 ggaccggtca tgcgaggcct tcgagggcgg aggccacctc cgcggccgcc ttcacgtcgg
     1561 cgcaccggaa cagcgcgctc agacccgagc ggctcatcag ggcgaggaag ccattgcact
     1621 cggcccagtc gccgccggcg ccgatctcgg tgacctcgcg cacgtcgccg cccttcacct
     1681 tcagatctcg atactcgatg ccccacgcca tctgcgtgtc gtagacgagg atccggtcgg
     1741 ggaagaagta cagggtctgt cggccagcgc gcagcgctgg aaccctgatg ttcgagcgga
     1801 cgcgcggcgg cagggcgagc accggcacga cgaggcggcg gtggcggcgc tggccgtcgg
     1861 ttgtccgccg gccgagatgc cagatcggac cgctcgtctt cagacgactg aaggccgtca
     1921 cgaggctccg atacgccacc gtcgcgtgat cgtcgagcac gtactcgacg gcctcgctgt
     1981 agtactcgat ctcgcgccgg ctcaccacca cgaccgcgag gaccgccagg gacccgaaga
     2041 gcagcgtggc gcccgcgggc gcgccgaagg cgagtccttc gatgaccgcg agtccgaaca
     2101 ccgctgccag caccatcgcg agcaccggcc gccgcatgta cgcgcggttg agcgcgcgca
     2161 gcaccgcgtc ggccgatgtg tcgagcatcg tctgcggctc cggccgtggc gactgcggct
     2221 ccgtgccccg gcgccgccgc atgacgggct cgtgcgtcgc gcttgctcgc ggatgtgacg
     2281 aggcgcggta cagcgggccg ccacgcgcca cgtggaccga ggcccggtgt gggcccagcc
     2341 cgacccgcct gacgcctccc gtcgcgtcac cgtcgaatgt cgtcctggcg tgcctgaacg
     2401 cagtgaacgt catcggacct cccgagccgc gtggcattgc atcgtggaca gcgggtaccc
     2461 gccgacgggg agcaattacc gggccggctg cggagcgatg cgctcacggt gacttacggc
     2521 gacccgcatg gacgatcggg cccgctcgcg ccaatttgcg cgcccgcgtg tcaggacgct
     2581 gacgatgtga gggcgggagg acgacgcgcc ccgcgaggcg gcgcgagacg ctaagggggg
     2641 gatgcctccg tggtgagcgc caggcgctgc agtagctggc tcaccggcgc cgcgaacatc
     2701 tgcgcggcga aaaagagcgt gaggagccag atgaccgccg cggccacggt gcggcgactc
     2761 atcgcgtcgg cggcgtgagc cgttcgagcc cgcccgcgta cgggcgcagc acgggcggga
     2821 tggtcacggt gccatccgcc tcctggtagt tctccagcac ggcgaccagc gtgcgcccca
     2881 ccgcgagccc cgagccgttg agcgtcgcgc agaactcggg cttgccgccg ctctccggct
     2941 tgtagcggat gtcggcgcgg cggccaccga aggcgtcata gttggagcac gacgagatct
     3001 cgcggtagcg gccctggccg ggcagccaca cctcgatgtc gtagcccttg gcggcgctga
     3061 acccgaggtc gccgacgcag cgctccacga cgcggtaggg cagctcc
//
LOCUS       DATIAZ010000307        14608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1404494, whole genome shotgun sequence.
ACCESSION   DATIAZ010000307 DATIAZ010000000
VERSION     DATIAZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 14608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14608
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404494"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VLF19_10725"
     CDS             <1..633
                     /locus_tag="VLF19_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE93771.1"
                     /translation="PQRGRILFEGRDLVGLKPYRRAALGISRTFQKIELFGGMTVLDN
                     IRLGRHLHMRSGLLAGALYLGPASREEIEHREFIEREIIDFLEMTHLRDKVVQMLPYG
                     LQKRVELGRALALNPRLLLLDEPMAGLNLEEVEDIARFIIDINEEERWRVTCILVEHD
                     MGVVMDISHRVAVLNFGEKIAEGTTEQVQAEPLVHQAYLGEEPGEYASRR"
     gene            611..2548
                     /locus_tag="VLF19_10730"
     CDS             611..2548
                     /locus_tag="VLF19_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93772.1"
                     /translation="MSTPPAGKGRPITPDLTLPKLLLETARRYGDGKVAMREKEFGIW
                     RPITWRRYLEEVRDLALGLTALGLTAGDKVALIGHNRPEGLWAEMAALSVGGVVVWLY
                     QDALLEEVQYVVDHSDARLLIGEGQQEVDKALAIKPRCPRLERIVWDDPKGMRGYDDP
                     ALMSLRDVRRRGKELAARDPEAFERLVARGRGEDVALLFYTSGTTSKPKGALLTHTNM
                     LKMGQNLMGVDPCEESDDFVSFLPFSWIGEQMMSISCGLQAGFTLNFPEDPETVQHDI
                     REIGPQVLFSPPRIYEQMVRTVQVKVLDAPLLKRRVYGWALKVGSEVADLVFAKKPVP
                     RTLGLERWLADLLVFRKLRDHLGLARIRDAYSGGAALGPDHLRFFHGIGVNLKQIYGQ
                     TEIAGISVVQRDGDVKFDTSGLPLPETEVRISADGEILSRSPCVFKGYYKMPEETERT
                     LEDGWLHSGDIGFIDGDGHLVVFDRRKDVMTLADGSRFSPQYIETRLKFSPYIKDAWI
                     VGHGRAAVNAVVCIDAGVVGKWAEDHHIPYTSYPELSQKGEVRALIADAVGHVNKHLP
                     AAARVRRFVNLHKEFDADDEELTRTRKLRRTFLEERYRDIVEGLYSDADAIHLDTTVT
                     YEDGRVSRTKTTLQVVDVSGA"
     gene            2548..3432
                     /locus_tag="VLF19_10735"
     CDS             2548..3432
                     /locus_tag="VLF19_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93773.1"
                     /translation="MRIFVELVLSGIMVGSIYALVALGWTLIYKCSGVLNLAMGELTL
                     IGAYVCLTYYQWGFPFPVAVLATLITGFVLGLATERLFLRRMIGEPVLAIIMITTGLA
                     FFFRAMVGFIWGTDTVVFTPPVFPQEPLRFFGMVFGQVYLWSFVAAIVLLVVFVWFFE
                     RSRWGLSMQATADDEMAALSLGVSAKRVYALAWAIAFMAAGVGGTLLGNINGLNISVG
                     YLGLLVLPAVVLGGLNSIPGAIVGGLVIGVLQNLSDAYVGPLTQGGVKEIAPFVFMVV
                     VLLFRPYGLWGWVRIERV"
     gene            3434..4498
                     /locus_tag="VLF19_10740"
     CDS             3434..4498
                     /locus_tag="VLF19_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93774.1"
                     /translation="MSYLPSGVYHETYAASHGWWPTTFVQGKMALLLLFVATLPLYAN
                     LYVVSVANITLYTVLSALGVQLLTGYCGQITLGHAAFIAVGAYVTAMGVLFWGLPYPV
                     AAVLGCLLAGLWSVVFGLPSARIKGFYQIMTTMAAQFITVDFIITHYVSRVGGREHYF
                     SLPPGTITIGGFPVDDEIKVYYLAAVLTIICFVALANVLRTKVGRAWIAIRDNDIAAE
                     AMGVNVVAYKLLAFFAAGVLAGVAGAVWIPSLLFVSPEHFQLSWGLWVVGVILIGGLG
                     SLHGVVFGSLFVTVILELLKLAVIPLSDYYPALAERFLYVKEAAFGLAIVLFLLYEPR
                     GLAYRWQQIKNYFNLWPFAY"
     gene            4543..5790
                     /locus_tag="VLF19_10745"
     CDS             4543..5790
                     /locus_tag="VLF19_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93775.1"
                     /translation="MTRLVLWMVALALSLPLFADQGWAQQIKLGSLNDLTGPTSDVGK
                     DVALGIREAVQYVNDTGGINGKRIRLYLYDYGYRVPEAITTYKRFRDFDKVVGVLGWG
                     TGDTEALSPTVGKDRLPYLSHSFAAKLTDPKIAPYNFVYGTDYSSNARAAMTAWFDEV
                     WMKDAKYREARSKGEKPRVVMFYAFPVPYSSAPIKALKNHAQLLGFEVGPDQDVSLTA
                     LDTKSQVLATKGFRPHLAWHGNTTMSVAATIKDSHALGLGADHIVNNWGYDENLVRLA
                     GPAAEGVMGIAVTAFYGAKVPLMDKVVEYARKINAGVPQEARMVRTTQAWAQVLMMAE
                     GMKRADKAGKLTGPGLREAFETLRGWTPGLGLPPVTLTASDHRPGGVIPVYRVRGGKF
                     ELVQNVDLKGRWPKLWDGEWFGY"
     gene            5813..6607
                     /locus_tag="VLF19_10750"
     CDS             5813..6607
                     /locus_tag="VLF19_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93776.1"
                     /translation="MLKVSNIEVLYHEVILALKGVSLEVPPQGIVALLGANGAGKSTT
                     LKAISGLLKHENGRVAEGAIELLGERIDRKSAEEIARMGIIQVIEGRRVFPHLTVEQN
                     LKVGGHLRRDGGSITAGLDMVYEYFPRLRERRRVAAGFASGGEQQMLVIGRALMAQPK
                     LLLLDEPSMGLAPLLIREIFEIVTRLNRQAGIPILLVEQNAKLALSVAQYAYVLENGR
                     VVMDGDSEKLRENPDIRDFYLGLTDLGTRKSFRSIKHYKRRKRWVS"
     gene            6622..7188
                     /locus_tag="VLF19_10755"
     CDS             6622..7188
                     /locus_tag="VLF19_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93777.1"
                     /translation="MKRSVVYGLSLLLLVAVAGCATMGPGGAQASAQPQVKLERVEVT
                     SYFPYAAPPARVPLILAFVFNVTNPNDVPITMDELRFAYAFEAKGEYFTLNSPAVYDV
                     MSIPGRTTNTVRVVSVLDSAIVPATLAVTQGFRLQQLGLKGPDVVKDWWERIGDFGYG
                     IRVTEGVANFTAGKQAMLVTFQDSFPKK"
     gene            7250..8773
                     /locus_tag="VLF19_10760"
     CDS             7250..8773
                     /locus_tag="VLF19_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93778.1"
                     /translation="MAVHGFTVYDLVARGAAVHGAAPALIQGERELSFRELLRRVDVL
                     AGGLAALGVGRGDRICVLAQNDVAYVELYGACARQGIVAYPINWRLTAQEVERVVERA
                     GPAMMVVDASTLPVVAGWPERKTAIAHWYQLGESAAAPGFTPFAALYRAAGETSPADV
                     SPSDPFAVISTAAVDVVPRGAVLTHANVITANLTAMACIGHTAADRYLVALPLFHVTA
                     LGGTLAHMHAGGASVVMSRFDPEEAVRLIDRHRVTHVSDFPPILTTLLDAAEKLGSPL
                     SSLKHVSGLDASQTIQRLHERTRAQFWTGFGQAETSGFVSLQRAADRPGAAGKPVPSA
                     RVALVDEYDREVPVGTPGEIVVRGPLVFDGYFGQPDVTAYTLRNGWHHTGDVGRFDAD
                     GYLHYVKRKPEKELIKPGGENVYPAEVETVIMQMTGVSGVCVYGIPDARWGEAIKAVV
                     EVKAGGRYTAQQVSDFVASKIARFKKPHLVVFTDALPRTAEGAVDRDGVKTTWGSAP"
     gene            8888..9733
                     /locus_tag="VLF19_10765"
     CDS             8888..9733
                     /locus_tag="VLF19_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSE93779.1"
                     /translation="MAAPDLVAIGHVTLDRFENEVRPGGSALYAAVTAHRLGLSAGIL
                     TSHGGDFPLEAVPPQIEVVSVPAPRTTLFAHRVHDGRRALRIEAAARPLSGADVPQDW
                     REAEMVLLAPVIAEVDVDLIAAFPEAAVGADAQGWLRGVGPGGEVVAQPWTPPRALFG
                     RLQALFLSREDVLGHEAQVTEWIQRLPVVVVTAGADGALLYVNGERYEVRPRKSDEID
                     PTGAGDVFAAAFMVRYRLDGDPWQASEVAACAAGLSVEGEAWSAVPDAAGLERALGAY
                     RAAYS"
     gene            9809..12661
                     /locus_tag="VLF19_10770"
     CDS             9809..12661
                     /locus_tag="VLF19_10770"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HSE93780.1"
                     /translation="MPNALKELLRYRQSVWLDYIRRSLITGGELRRLLDEDGLRGVTS
                     NPAIFEKAIAGSADYESQLKELARRRDLDAKGAYEVLAVRDIQDVADILKPIYEQSGR
                     RDGYVSLEVSPDLANDTRGTLDEARRLWATVARANVMIKVPATPAGVPAIRELIGDGI
                     NVNVTLLFALEAYEAVAEAYIAGLEALAARGGDPSRVESVASFFISRIDSLVDSLVEA
                     RLKASDNALEQGLLKGLPGRVAIANAKLTYQRYKEIYRGPRWAALAARGARTQRLLWA
                     STSTKNASYRDVIYVEELIGPDTVNTIPPATYDAFRDHGRLRASLEDDLEDAHHTMDS
                     LAESGISMKQVTDQLLVEGVKVFVDAFAKLMGAVQKRLATPGEEVTRQAWTLPADLTA
                     DVKTSLKDWADGGKVHRLWARDASLWTGADEGRRLGWLGITDDQLAQIDHLRQVAEDV
                     RRGGFTHAVLLGMGGTVLGAEALARTFGRIAGSPELHVLDSTDPAQIRAVERALDPAR
                     TLFIVASKSGSTLEPNILMRHFLERVRGAVGPAEAGRRFMAITDPGSKMQHVAEAGGF
                     RMIFLGEPSIGGRFSTLSDFGAVPAAVMGLDVARLLGRAEEMAHACAASVPVEENPGV
                     VLGTIMGVLGRRGRDKVTLIVSPAVQALGTWIEQLLTASLGKAGRGLLAVEGETAGVP
                     DVYGADRLFVYLRSPAAPDAGQDAAVAALQTAGQPVVRITIDDAYDLGAEFFRWQFAT
                     AVAGSVLGLNPFDQPDIEGGKVAARKLAAAYEKTGALAPETPIRQGDGLTLFADAGNA
                     AALDAASRERTVAAALKAHLGRVKAGDYVALLPYLEMSAANETDLGALRLVLRDRLKV
                     ATSLGFGPRYLHSSGQAYKGGRDSGVFMQITADDAADLPVPGHKYTFGVVKAAQARGD
                     LEMLAERGRRAIRVHVGRDVKAGLAALRRAVEQALG"
     gene            12664..13035
                     /locus_tag="VLF19_10775"
     CDS             12664..13035
                     /locus_tag="VLF19_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HSE93781.1"
                     /translation="MSRAIRISAGSISVQATLDGSRTASAIWDALPLEARVETWGEEI
                     YFDVGLDLADESPRPVVEMGDLAYWPPGQAFCVFFGRTPASRGDECRPASPVSVFGRV
                     DGDASVLKKVRAGTRVRVERV"
     gene            13072..13722
                     /gene="fsa"
                     /locus_tag="VLF19_10780"
     CDS             13072..13722
                     /gene="fsa"
                     /locus_tag="VLF19_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HSE93782.1"
                     /translation="MKIFLDTANIDEIREGVALGLVDGVTTNPSLIAKEKRAFRPAVE
                     EICATVPGDVSLEVVATDFEGMVKEGQELAQIADNVVVKCPLTKDGLKAVRYLTSKDI
                     RCNQTLCFSGAQALLSAKAGAFYISPFLGRLDDIGHVGMDLIREICEIYRVQGFKTQV
                     LAASIRNPLHVIDAAKAGAHVATLPFSVLEQLMRHPLTDVGVKKFLDDWAKLGEKI"
     gene            complement(14001..>14608)
                     /locus_tag="VLF19_10785"
     CDS             complement(14001..>14608)
                     /locus_tag="VLF19_10785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSE93783.1"
                     /translation="PHRHPVLAAKALATLDHLAPGRVILGAGVGWMREEIELLGAPYD
                     RRGAWSDEAIRIMRACWRDERVAFRGAFFSFDPVGVAPKPARGTIPVWIGGHTPAALR
                     RVAELGDGWHAAFPTAAALAPTLERLREACHKAGRDPRSVTLSARVGLSARRPADELI
                     ADLRALRDLGVAHVVLESRARDLTDMVATYERFASDIRPRL"
BASE COUNT         2208 a         4992 c         5134 g         2274 t
ORIGIN      
        1 ccgcaacgcg ggcgcatcct cttcgaaggc cgggacctgg tcggcctcaa gccctaccgg
       61 cgcgcggcgc tcggcatctc gcgcaccttc cagaagatcg agctgttcgg cgggatgacg
      121 gtgctcgaca acatccgcct cggtcgccac ctccacatgc ggagcgggct cctcgccggc
      181 gctctgtacc tcgggcccgc gagtcgtgag gagatcgagc accgggagtt catcgagcgg
      241 gagatcatcg acttcctcga gatgacgcac ctgcgcgaca aggtggtgca gatgctgccc
      301 tacgggctcc agaagcgggt ggagctggga cgcgcgctgg ccctgaaccc caggctcctc
      361 ctgctcgacg agccgatggc ggggctcaac ctggaggagg tcgaggacat cgctcgtttc
      421 atcatcgaca tcaacgagga ggagcgctgg cgggtcacgt gcatcctcgt cgagcacgac
      481 atgggcgtcg tcatggacat ctcccaccgg gtcgccgtgc tgaacttcgg cgagaagatc
      541 gccgagggca cgaccgagca ggttcaggcc gagccgctgg tgcaccaggc atacctgggc
      601 gaggagccgg gtgagtacgc ctcccgccgg taagggccgg cccatcacgc cggatctcac
      661 gctgcccaag ctcctcctcg agaccgcccg ccgctacggc gatggcaagg tcgccatgcg
      721 cgagaaggag ttcgggatct ggcgaccgat cacgtggcgg cgatacctgg aggaggtccg
      781 cgacctcgcc ctggggttga ccgcactcgg gctcaccgcc ggggacaagg tcgcgctcat
      841 cggtcacaac cggccggaag ggctctgggc cgagatggcg gcgctctccg tgggcggcgt
      901 cgtggtgtgg ctctatcagg acgcgctgct ggaggaggtg cagtacgtcg tcgaccattc
      961 cgacgctcgc ctcctgatcg gcgagggtca gcaggaggtc gacaaggcgc tcgccatcaa
     1021 gccgcgctgc cccagactcg agcgcatcgt ctgggatgat cccaagggca tgcgcgggta
     1081 cgacgatccc gcgctcatga gcctgcgcga cgtgcggcgg cgcgggaagg agctggcggc
     1141 gagggatccc gaggccttcg agcgcctggt ggcgcgcggc cggggcgagg acgtcgcgct
     1201 cctcttctac acctcgggaa ccacgtccaa gccgaagggc gccctcctca cgcacacgaa
     1261 catgctgaag atggggcaga atctcatggg cgtcgacccg tgcgaggaga gtgacgactt
     1321 cgtgtcgttc ttgcccttct cctggatcgg ggagcagatg atgtcgatct cctgcgggct
     1381 ccaggccggc ttcacgctga acttccccga agaccccgag acggtccagc acgacatccg
     1441 ggagatcggg ccccaggtgc tcttctcgcc gccgcggatc tacgagcaga tggtgcggac
     1501 ggtgcaggtc aaggtgctcg acgcgcccct cctcaagcgg cgcgtctatg gctgggcgct
     1561 gaaagtgggg agcgaagtgg cggacctcgt cttcgccaag aagcccgtcc cgcgcacgct
     1621 cgggctcgag cgctggctgg ccgacctgct cgtcttccgc aagctgcgcg accacctcgg
     1681 cctcgctcgc attcgcgatg cctactccgg cggcgccgcg ctcggccccg accacctccg
     1741 cttcttccac ggcatcggcg tgaacctgaa gcagatctac gggcagacgg agatcgccgg
     1801 catctcggtg gtgcaacgcg acggcgacgt gaagttcgac acttcggggc tgcccctccc
     1861 ggagacggag gtgcgcatct cggcggacgg ggagatcctg tcgaggagcc cgtgcgtctt
     1921 caagggctac tacaagatgc cggaggagac agagcgcacg ctcgaggacg gctggctgca
     1981 ttccggcgac atcgggttca tcgacggcga cggccacctg gtcgtcttcg accgccgcaa
     2041 ggacgtgatg acgctggccg acggcagccg gttctcgccg cagtacatcg agacccgcct
     2101 gaagttcagc ccgtacatca aggacgcctg gatcgtcggc cacggccgcg cggccgtgaa
     2161 cgcggtcgtc tgcatcgacg ccggcgtggt ggggaagtgg gcggaagacc accacatccc
     2221 gtacacttcc tatcccgagc tgtcccagaa gggggaggtc cgcgccctca tcgcggacgc
     2281 cgtgggccac gtcaacaagc acctgccggc agccgcccgc gttcggcgct tcgtgaatct
     2341 ccacaaggag ttcgatgccg acgacgagga gctgacgcga acacgaaagc tccgccgcac
     2401 gttcctcgag gaacggtacc gcgacatcgt ggaggggctc tattcagacg ccgacgcgat
     2461 ccacctggat acgaccgtca cgtacgaaga tggtcgggtt tcgcgcacga agaccacgct
     2521 ccaggtcgtg gacgtgtcgg gcgcctgatg cggatcttcg tcgagctggt gctgtcgggg
     2581 atcatggtgg gctcgatcta cgccctggtc gcgctgggct ggacgctcat ctacaagtgc
     2641 tcgggcgtcc tgaacctcgc catgggcgag ctcaccctca tcggcgccta cgtctgtctc
     2701 acgtattacc agtggggctt cccgttcccg gtcgcggtgc tcgccacgct catcacgggc
     2761 ttcgtcctcg gcctggccac ggaacggctg ttcctgcgcc ggatgatcgg cgaaccggtc
     2821 ctggccatca tcatgatcac caccggactc gcgttcttct tccgggcgat ggtggggttc
     2881 atctggggca ccgacacggt ggtcttcacc ccgccggtgt tcccccagga gccgcttcgg
     2941 ttcttcggca tggtgttcgg gcaggtctac ctctggagct tcgtggcggc gatcgtgctc
     3001 ctggtggtct tcgtgtggtt cttcgagcgc agccgctggg gtctctcgat gcaggcgacg
     3061 gcggacgacg agatggctgc gctctctctc ggcgtgtcgg ccaagcgcgt gtacgcgctg
     3121 gcgtgggcca tcgccttcat ggcggcgggg gtggggggca cgctcctggg caacatcaac
     3181 ggcctcaaca tctccgtcgg ctacctcggc ctcctggtgc tgccggccgt cgtcctgggc
     3241 gggctcaact cgatccccgg ggccatcgtc ggcggcctcg tcatcggcgt ccttcagaat
     3301 ctctcggacg cttacgtggg tccgctcacc cagggtgggg tcaaggagat cgcgcccttc
     3361 gtcttcatgg tcgtcgtcct gctcttccgg ccctacggcc tctggggctg ggtcaggatc
     3421 gagcgggttt gacgtgagct acctgccgag cggcgtctat cacgagactt acgcggcgag
     3481 ccacgggtgg tggccgacga cgttcgtcca gggcaagatg gcgctcctcc tgctgttcgt
     3541 cgccaccctg ccgctctacg ccaacctcta cgtcgtctcc gtggccaaca tcacgctgta
     3601 cacggtgctc tcggcgctcg gtgtccagct cctcaccggg tactgcggcc agatcacgct
     3661 gggccacgcc gccttcatcg ccgtgggcgc ctacgtcacc gccatggggg tgctcttctg
     3721 gggactgccc tatccggtgg ccgcggtgct cgggtgcctg ctcgccggcc tctggtcggt
     3781 cgtgttcggg ctgccgtcgg cgcgcatcaa gggtttctac cagatcatga ccacgatggc
     3841 ggcgcagttc atcaccgtgg acttcatcat cacccattac gtcagccggg tgggcggtcg
     3901 cgagcactac ttctcgctgc ctcccggcac catcaccatc ggcggcttcc cggtggacga
     3961 cgagatcaag gtctactatc tggcggcggt gctgaccatc atctgcttcg tggcgctggc
     4021 caacgtgctc cgcaccaagg tggggcgcgc ctggatcgcc atccgggaca acgacatcgc
     4081 ggcggaggcc atgggcgtca acgtcgtggc ctacaagctc ctggcgtttt tcgccgccgg
     4141 tgtcctggcc ggcgtggcgg gggcggtgtg gattccctcg ctgctgttcg tgagcccgga
     4201 gcacttccag ctctcgtggg gcctctgggt ggtgggcgtc atcctcatcg gcggcctcgg
     4261 gagcctgcac ggcgtcgtct tcggctccct cttcgtcacc gtcatcctgg agctgctgaa
     4321 gctcgccgtg atcccgctct ccgactacta cccggccctc gccgagcgat tcctttacgt
     4381 gaaggaggcc gccttcgggc tggccatcgt cctcttcctg ctctacgagc cccgcggcct
     4441 cgcctaccgc tggcagcaga tcaagaacta cttcaacctc tggccgttcg cctactgatg
     4501 cgacggtcgg gccgatacgc gcaggtgaca aggaggcagc ccatgacacg actggtgctc
     4561 tggatggtcg ctctcgccct ctcgctgccg ctgttcgcgg accagggctg ggcgcaacag
     4621 atcaagctcg gctcgctgaa cgacctcacc gggccgacat cggacgtcgg caaggacgtg
     4681 gcgctcggca tccgggaggc cgtccagtac gtgaacgaca cgggcggtat caatggcaag
     4741 cggatccgcc tgtacctcta cgactacggc taccgcgtgc cggaggcgat caccacctac
     4801 aagcggttcc gcgacttcga caaggtggtg ggtgttctcg gctggggaac aggcgacacc
     4861 gaagcgctct ccccgacggt gggcaaagac cgcctcccgt acctgtcgca ctccttcgcg
     4921 gcgaagctca ccgatcccaa gatcgcgcca tacaacttcg tctacggcac ggactactcg
     4981 tcgaacgccc gcgccgccat gacggcctgg ttcgacgaag tctggatgaa ggacgcgaag
     5041 taccgggagg cacggtccaa aggcgagaag ccgcgggtgg tgatgttcta cgccttcccg
     5101 gtgccgtact cgagcgcccc catcaaggcc ctcaagaacc acgcccagct tctcggcttc
     5161 gaagtggggc cggatcagga cgtctcgctc accgcgctcg acaccaagag ccaggtcctg
     5221 gccacgaagg gcttccggcc gcacctggcc tggcacggca acacgacgat gtcggtggcg
     5281 gcgacgatca aggactccca tgccctcggt ctcggcgccg accacatcgt gaacaactgg
     5341 gggtacgacg agaacctggt gcgtctggcc ggcccggccg ccgagggcgt catgggcatc
     5401 gccgtcaccg cgttctacgg cgccaaggtc cccctcatgg acaaggtggt cgagtacgcg
     5461 aggaagatca acgccggcgt accccaggag gcccggatgg ttcgcaccac ccaggcgtgg
     5521 gcccaggtcc tcatgatggc ggagggcatg aagcgcgccg acaaggccgg caagttgacg
     5581 ggccccggcc tgcgggaggc gttcgagacg ctgcgcggat ggaccccggg gctgggcctg
     5641 cccccggtga cgctcacggc gagcgaccac cgtccaggcg gcgtcattcc agtctatcgg
     5701 gtcaggggcg gcaagttcga gctggtgcag aacgtcgacc tcaaaggccg gtggccgaag
     5761 ctgtgggatg gcgaatggtt cggatactga agtgacgcgc gctgtctgag ccatgctcaa
     5821 ggtcagcaac atcgaggtgc tctatcacga ggtcatcctc gccctgaagg gcgtgtcgct
     5881 cgaggtgccc ccgcagggca tcgtcgcgct gctcggcgcc aacggcgccg ggaagtcgac
     5941 gacgctcaag gcgatctcgg gtctgctcaa gcacgagaac ggacgggtcg ccgagggggc
     6001 gatcgagctc ctgggtgagc ggatcgatcg gaagtcggcc gaggagatcg cgcggatggg
     6061 gatcattcag gtgatcgagg gccggcgggt ctttccccac ctgaccgtcg agcagaacct
     6121 gaaggtcggc ggtcacctgc gccgggacgg cggcagcatc acggctgggc tggacatggt
     6181 ctacgagtac ttcccgcggc tccgtgaacg gcgacgcgtg gcggcgggct tcgccagcgg
     6241 cggcgagcag cagatgctcg tgatcggccg ggcgctcatg gcgcagccga agctcctcct
     6301 cctggacgag ccgtccatgg gtctggcgcc gctgctcatc agggagatct tcgagatcgt
     6361 cacgcggctc aaccgccagg cggggatccc gatcctgctc gtggagcaga acgccaagct
     6421 cgccctctcg gtggcgcagt acgcctacgt gctcgagaac ggccgtgtgg tgatggacgg
     6481 cgattccgag aagcttcgcg agaacccgga tatccgcgac ttctacctcg gcctcacgga
     6541 tctcggaacg cgcaagagct ttcgttccat caagcactac aagcgacgca agcggtgggt
     6601 atcgtgaaaa gggggagagc catgaagcgg agcgtcgtct acggcctgag tctcctgctg
     6661 ctcgtcgccg tcgccggatg tgcgaccatg gggccgggcg gggcccaggc cagcgcccag
     6721 ccgcaggtga agctggagcg cgtggaggtg acgagctact tcccctacgc cgcgccgccc
     6781 gcccgggtgc cgctgattct cgccttcgtg ttcaacgtca cgaaccccaa cgacgtgccg
     6841 atcaccatgg acgagctccg gttcgcctac gccttcgagg cgaagggcga gtacttcacc
     6901 ttgaactcac cggccgtcta cgacgtcatg agcattccgg gccgcaccac caacacggtg
     6961 cgggtggtga gcgtcctgga ctccgccatc gtgccggcca ccctggcggt gacccagggc
     7021 ttccgcctcc agcagctcgg cctcaagggg ccggacgtgg tgaaggactg gtgggagagg
     7081 atcggcgatt tcggctacgg catccgggtc accgagggcg tggccaactt caccgcgggc
     7141 aagcaggcga tgctggtcac gttccaggac tcgttcccga agaagtgagc gcgcgctcgc
     7201 tctcctcggg ccgatgaaac agggcatcgt ggggcagtag gaattgcgca tggccgtgca
     7261 cggcttcacc gtctacgatc tggtcgcgcg cggcgcggcg gttcacggtg ccgcgcccgc
     7321 gctcatccag ggggaacgcg agctctcgtt ccgggagctc ctgcgccggg tggacgtgct
     7381 ggccggaggg ctggcggccc tcggcgtcgg ccggggtgat cgcatctgcg tcctggccca
     7441 gaacgacgtg gcctacgtcg agctgtacgg cgcctgcgcg cgacagggca tcgtcgccta
     7501 tcccatcaac tggcggctga cggcgcagga agtggagcgg gtggtggagc gcgccggacc
     7561 cgccatgatg gtggtcgacg cctccacgct gcccgtggtg gcgggctggc cggagcgcaa
     7621 gacggccatc gctcactggt accagctcgg cgagtccgcg gcggccccgg gcttcacgcc
     7681 cttcgccgcg ctgtaccggg cggccggcga gacctccccc gccgacgtgt cgccctccga
     7741 ccccttcgcc gtgatctcca ccgccgcggt ggacgtcgtc ccgcgcggcg ccgtgctcac
     7801 gcacgccaac gtgatcaccg ccaacctgac ggccatggcc tgcatcggcc acacggcggc
     7861 ggatcgctat ctcgtggcgc tgcccctatt ccacgtcacc gcgctcggcg gcacgctcgc
     7921 gcacatgcac gccggcggcg ccagcgtggt gatgtcgcgc ttcgaccccg aggaagcggt
     7981 gcggctgatc gaccgtcacc gcgtcaccca cgtctcggac tttccgccca tcctgaccac
     8041 cctgctggat gccgccgaga aactgggcag cccgctgtcg agcttgaaac acgtctccgg
     8101 cctcgacgcg tcccagacga tccagcgcct gcacgagcgc acccgcgcac agttctggac
     8161 cggcttcggg caggcggaaa ccagcgggtt cgtcagcctg cagcgcgcgg ccgataggcc
     8221 cggcgcggcc ggcaagcccg tgccctcggc gcgggtggcc ctcgtcgacg agtacgaccg
     8281 cgaggtcccc gtgggaacgc cgggagagat cgtcgtgcgc gggccgctgg tcttcgacgg
     8341 gtacttcggt cagccggacg tgaccgctta cacgctgcgc aacggctggc accacaccgg
     8401 agacgtcgga cgcttcgacg ccgacgggta tctccactac gtgaagcgca agccggagaa
     8461 ggagctgatc aagcccggcg gagagaacgt ctaccccgcg gaggtggaga cggtcatcat
     8521 gcagatgacg ggcgtgagcg gggtgtgcgt gtacggcatt cccgacgccc ggtggggcga
     8581 ggccatcaag gccgtggtgg aggtgaaggc gggcggccgc tacaccgctc agcaggtgag
     8641 cgacttcgtg gcttcgaaga ttgcgcgctt caagaagccg cacctggtcg tattcacgga
     8701 cgccctgccg cggaccgcgg agggcgcggt cgatcgtgac ggcgtcaaga ccacgtgggg
     8761 cagcgccccg tgaggcctcg ggtggtcgat ccgagccagg tgtgacagcg tcgcgaccgg
     8821 tgccgcgtcg gacgacgcgg gatcgtcgcg cgcccggccc gccggctcga acggaggaag
     8881 accctctttg gccgcccctg atctcgtcgc catcgggcac gtgacgctgg accggttcga
     8941 gaacgaggtc cgcccgggcg ggtcggcgct ctacgccgcc gtcacggcgc accggctcgg
     9001 gctcagcgcc ggcatcctga cgagccacgg cggcgacttc cctctggagg ccgtgccgcc
     9061 gcagatcgag gtggtctccg tgccggcgcc tcgcacgacc ctgttcgccc accgcgtgca
     9121 cgatggccgc cgcgccctgc ggatcgaggc cgcggcgagg ccgctctcgg gggccgacgt
     9181 gccgcaagac tggcgcgagg ccgagatggt cctgctggcg cccgtgatcg cggaggtcga
     9241 tgtcgacctg atcgcggcgt tcccggaggc ggcggttggc gcggacgcgc aggggtggct
     9301 gcgaggcgtg ggccccggcg gcgaggtggt ggcgcagccg tggacgccgc cgcgggcgct
     9361 cttcggacgg ctgcaggcgc tcttcctgag ccgagaggat gtcctgggcc acgaagcgca
     9421 ggtgaccgag tggatccagc gactgcccgt cgtcgtcgtc acggccggag cggacggcgc
     9481 gctcctctac gtcaacggcg agcgctacga ggtccgtccc cgcaagagcg acgagatcga
     9541 tccgaccggc gcgggcgacg tgttcgcggc ggcgttcatg gtccgttacc ggctcgacgg
     9601 cgatccctgg caggcctccg aggtggcggc ctgcgccgcc gggctgtcgg tggagggcga
     9661 ggcctggtcg gcggtgcccg acgccgccgg gctggaacgt gccctcgggg cgtaccgcgc
     9721 cgcctattct tgacggcggg ggggcgacgg gtcacactag cgcgcgcatg ttcagcgcga
     9781 gctcagcgct cgctcacggg agggcgtgat gcccaatgcg ttgaaggaac tgctgcggta
     9841 ccgccaatcg gtgtggctcg attacattcg gcgcagcctg atcacggggg gcgagcttcg
     9901 acggctcctg gacgaggacg gcctccgcgg cgtcacgtcc aacccggcca tcttcgagaa
     9961 ggcgatcgcc ggctcggccg actacgagag ccagctcaag gagctggcgc ggcggcgcga
    10021 cctcgacgcc aagggcgcgt acgaggtcct ggccgtgcgc gacatccagg acgtcgccga
    10081 catcctgaag ccgatctacg agcagagcgg ccggcgcgac ggctacgtga gcctcgaggt
    10141 ctcgccggat ctcgccaacg acacgcgagg cacgctcgac gaggcgcggc gcctgtgggc
    10201 caccgtcgcg cgcgccaacg tcatgatcaa ggtcccggcg accccggccg gcgtgcccgc
    10261 gatccgtgaa ctcatcggcg acggcatcaa cgtcaacgtc acgctgctct tcgcgctcga
    10321 ggcatacgag gccgtggccg aggcctacat cgccggcctc gaggccctgg ccgcccgggg
    10381 cggcgacccc agccgggtcg agagcgtggc gagcttcttc atcagccgga tcgacagcct
    10441 ggtggactcg ctggtcgagg cgcggctcaa ggcttcggac aacgcgctgg agcagggcct
    10501 gctgaagggc ctccccggcc gggtggcgat cgcgaacgcg aagctgacct accagcgcta
    10561 caaggagatc taccgcggtc cccggtgggc ggccctggcc gctcggggcg cgcgcacgca
    10621 gcgcctgctg tgggcgagca ccagcaccaa gaacgccagc taccgggacg tcatctacgt
    10681 cgaggagctg atcgggccgg acaccgtcaa cacgatcccg ccggccacct acgacgcctt
    10741 ccgcgaccac gggcgcctgc gcgcgagcct cgaggacgac ctcgaggacg cgcaccacac
    10801 gatggactcg ctggcggaga gcggcatctc gatgaagcag gtcaccgacc agctcctcgt
    10861 ggaaggcgtc aaggtcttcg tcgatgcctt cgccaagctg atgggggcgg tgcagaagcg
    10921 gctcgccaca ccgggcgagg aggtcactcg ccaggcctgg acgctgcccg ccgatctcac
    10981 cgccgacgtg aagacctcgc tcaaggactg ggcggacggt ggcaaggtcc atcggctgtg
    11041 ggctcgcgac gcgagtctct ggaccggggc cgacgagggc cggcgcctgg gctggctcgg
    11101 gatcaccgac gaccagctcg ctcagatcga tcacctccgc caggtggccg aagacgtccg
    11161 ccgcgggggg ttcacccacg cggtactcct cggcatgggc ggcaccgtgc tcggcgccga
    11221 ggcgctcgcg cggaccttcg gcaggatcgc ggggtctccc gagctgcacg tgctggactc
    11281 caccgatccc gcacagatcc gggcggtgga gcgcgcgctc gatcccgcgc gcacgctgtt
    11341 catcgtggcg agcaagtcgg gcagcacgct cgagcccaat atcctgatgc ggcacttcct
    11401 cgagcgcgtg cgcggcgcgg tcggtcccgc cgaggccggc cgccgcttca tggcgatcac
    11461 cgatccgggc tcgaagatgc agcacgtggc cgaggccggt ggcttccgga tgattttcct
    11521 cggcgagccg tcgatcggcg gccgcttctc gacgctgagc gacttcggcg ccgtccccgc
    11581 cgccgtcatg gggctcgacg tcgcgcggct ccttggtcgg gccgaggaga tggcccacgc
    11641 gtgcgcggcc agcgtgccgg tggaggagaa ccccggcgtc gtgctcggga ccatcatggg
    11701 tgttctcggc cgtcgcggtc gcgacaaggt cacgctgatc gtctcgccgg ccgtgcaggc
    11761 gctcggcacg tggatcgagc agctcctgac ggcctcgctc ggcaaggccg gccgcggcct
    11821 cctcgcggtc gaaggggaga cggcgggcgt gcccgacgtc tacggtgccg accgcctctt
    11881 cgtctatctg cgctcgccgg cggcgcccga cgcgggccag gacgcggcgg tggccgcgct
    11941 gcagacggcc gggcagcccg tggtgcgtat caccatcgac gatgcctacg acctcggcgc
    12001 cgagttcttc cgctggcagt tcgcgacggc ggtggccgga tcggtcctcg gcctgaaccc
    12061 gttcgaccag cccgacatcg agggcggcaa ggtcgccgcc cgcaagctcg ccgccgcgta
    12121 cgagaagacc ggcgcgctgg cgcccgagac gccgatccgc cagggcgacg ggctcacgct
    12181 cttcgccgac gccggcaatg cggcggcgct cgacgccgcg agccgcgagc ggacggtggc
    12241 cgccgccctc aaggcccatc tcggccgcgt caaggccggc gactacgtcg cgctcctgcc
    12301 gtacctggag atgagcgcgg ccaacgagac cgacctcggc gcgctccgcc tcgtgctgcg
    12361 cgaccggctc aaggtggcga cgtcgctcgg cttcggtccg cgatatctcc actcgagcgg
    12421 ccaggcctac aagggaggcc gcgactcggg cgtgttcatg cagatcaccg ccgacgacgc
    12481 ggcggacctg cccgtgcccg gccacaagta cacgttcggc gtcgtgaagg cggcccaggc
    12541 gcgcggcgat ctcgagatgc tggccgagcg cgggcgccgg gcgatccgcg tccacgtcgg
    12601 ccgcgacgtg aaggccgggc tcgcggcgct gcgccgggct gtcgagcagg cgctcgggta
    12661 gcgatgtcgc gggccatccg gatcagcgcc ggctccatct cggtgcaggc gacgctggac
    12721 ggatcccgca ccgcgagcgc gatctgggac gccctgcccc tcgaggccag ggtcgagacc
    12781 tggggagaag agatctactt cgatgtcggc ctggacctgg ccgacgagtc gcccaggccc
    12841 gtcgtcgaga tgggcgatct cgcgtactgg ccgccgggcc aggcgttctg cgtcttcttc
    12901 ggacggacgc cggccagccg cggcgacgag tgccggccgg cgagcccggt cagcgtattc
    12961 ggtcgcgtgg acggcgatgc gagcgtgctc aagaaggtcc gcgcgggtac ccgggtgcgc
    13021 gttgagcgtg tctgacggcg ggtgtacaag gagaggaggg gggatcggag catgaagatc
    13081 tttctcgata ccgccaatat cgacgagatc agggaggggg tcgcgctcgg cctggtggac
    13141 ggcgtcacga ccaacccctc gctcatcgcg aaggagaagc gcgcgttccg cccggccgtc
    13201 gaggagatct gcgccaccgt gcccggtgac gtcagcctcg aggtggtcgc gacggacttc
    13261 gagggcatgg tgaaagaggg ccaggagctg gcccagatcg ccgacaacgt cgtcgtgaag
    13321 tgccccctca ccaaggacgg gttgaaggcg gtccggtatc tcacgagcaa ggacatccgc
    13381 tgcaaccaga cgctgtgctt ctcgggtgcg caggcgctcc tgtccgccaa ggcgggcgcc
    13441 ttctacatca gcccgttcct cggccgcctg gatgacatcg ggcacgtcgg catggacctg
    13501 atccgcgaga tctgcgagat ctaccgcgtg cagggcttca agacccaggt gctcgcggcc
    13561 tccatccgca acccgctgca cgtgatcgac gccgccaagg ccggcgccca cgttgccacg
    13621 ctgcccttca gcgtgctcga gcagctcatg agacacccgc tcacggacgt cggagtcaag
    13681 aagttcctcg acgactgggc caagctcggc gagaagatct aggcctccgt tcgccggtga
    13741 acgcaccggc cggggtaggg tcgggggcgc gggtcggcga cccctgatgc gttgcgcctc
    13801 gtcgctctgg tgccatttcc atcttcaggt ggcgcggggt ttccgtgcgc ggggaacagg
    13861 tcggcggacc cgcgcccccg accccgcccc ggccggcggt cggcgagcat gggactcagg
    13921 aggctggagc ggcggccctg ccgccgcaac cccgtcctgg gcgaggctcg gagggggccg
    13981 tcgaggcccc cctttcatta tcagaggcgg ggccggatgt cggaagcgaa gcgctcgtag
    14041 gtcgcgacca tgtcggtgag gtcgcgcgcg cgggattcga ggacgacgtg ggcgacgccg
    14101 aggtcgcgaa gcgctcgaag gtcggcgatg agctcgtcgg ccgggcggcg tgccgacagg
    14161 ccgacccgcg cgctcagcgt caccgatctc ggatcgcggc cggccttgtg acatgcctcg
    14221 cgcaggcgct cgagggtcgg agcgagtgcg gccgcggtcg ggaaggcggc gtgccagccg
    14281 tcgccgagct cggccacgcg gcgtagcgcc gccggcgtgt ggccgccgat ccagaccggg
    14341 atcgtgccgc gcgccggctt gggcgccacg ccgacgggat cgaagctgaa gaaggcgccg
    14401 cggaaggcga cgcgctcgtc gcgccagcag gcgcgcatga tccggatggc ctcgtcgctc
    14461 cacgcgccac ggcggtcgta cggcgcgccc agcagctcga tctcctcgcg catccacccc
    14521 acgcccgcgc cgaggatcac gcgccccggc gccaggtggt cgagcgtcgc gagcgccttg
    14581 gccgccagga cagggtggcg gtgcggca
//
LOCUS       DATIAZ010000308        12702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_483657, whole genome shotgun sequence.
ACCESSION   DATIAZ010000308 DATIAZ010000000
VERSION     DATIAZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483657"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..945)
                     /locus_tag="VLF19_10790"
     CDS             complement(100..945)
                     /locus_tag="VLF19_10790"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HSE93784.1"
                     /translation="MRRGNPRFDAVDHLLALDPGARGIPSFFVPGAAGAAACTLRSAR
                     RVLVVTGFCVPPGLSETDGPPGAAVLGRALRRLGARVRYVTDAPVVAPLAAALDALGE
                     PAEVEVFDGDARALLARERPTHLVAVERPGRARSGDYLSARGESVAPWNSPMDELFLR
                     VGKTGPITIAVGDGGNEIGMGNVRARLARMGPLMAKISSVVRVDHLVVAGTSNWGAYG
                     IVAGLARLARRPLLHTPADERRLVEACVKAGAVDGLVRRAEPTVDGLPLEVHAAFVEL
                     LRLAV"
     gene            complement(959..2812)
                     /gene="treZ"
                     /locus_tag="VLF19_10795"
     CDS             complement(959..2812)
                     /gene="treZ"
                     /locus_tag="VLF19_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HSE93785.1"
                     /translation="MRARLLSPPASPRPGSRGLPLDRMNGPGASIHEGGATFRVWAPR
                     CRTVDVLLDGGPPQPLTAVGEGLFELGVKRVRAGARYQYRLDGRRYRPDPVSRWQPEG
                     VHGPSVVVDTAAFAWTDERFRGHALADLVFYELHVGTFSTAGTFEGVIPYLDRLAELG
                     VTAVELMPIAEFPGSRSWGYDGVHLFAPQSTYGGPRGLSRLVDACHARGLSVFLDVVY
                     NHLGPEGNYLAEYGPYFTDRYETPWGQAVNFDGPDAAGVRRHFVENARMWVREFHVDG
                     LRLDAIHSIFDSSPVHILIEIAAAVREEAASLGRPGHVTAESHDNDRAIVTPAEAGGI
                     GLDAVWADDFHHALHVRLTGETTGYYVDFADGQHLPRALAEGFAYQGEPSVYFGRPRG
                     TPSGDVEGERFVLCTQNHDQVGNRAQGDRLATIVPFEAAKLAAALLMVAPALPLLFMG
                     EEYGETAPFQFFTSFLDPGLAAAVRRGRAAEFSRFGWTGAISDPQAPDTYVRSRLNHA
                     LAGAPRHRALREFYRAWLALRRQHPALGARGKSRTRAALDETGSVLVVDRAGPSGEAV
                     QCVANLGAKPQIWPAPPRSRVLLDSAEARFGGPERVRPLEPWQVLLYALSG"
     gene            2811..4499
                     /locus_tag="VLF19_10800"
     CDS             2811..4499
                     /locus_tag="VLF19_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HSE93786.1"
                     /translation="MSYPFCLVLHTHLPMVVNHGRWPHGSDWLCEATFECYLPLLDTV
                     HRLLADGIKAKWTINVSPVLTEQLASPEFQRELSFYYENVRRACADSRQHFGREGNHQ
                     IVKLTHFWEEFYERMWELHRRIGGDIPGTLADLERQGAIEVITCAATHGYLPLLSRDE
                     SIRLQLRTAVETHRRHYGRRPRGIWLPECAYRPRYAWTPPTGREQDKTRRMRPGLEEM
                     LAANGLQYFVADSHLIAASEPVFLYRDYVPLRGQLGDPVAEVPRTNGRTPYAPWRVAS
                     RGGTGSAVAFFRDPKTTLQVWSRAHGYPGDFHYLEFHKKHHPGGLRFWRISDAANDLG
                     SKQPYDPGAAMERVEWQARHFLELVRDTAQAAGGDGPALVCSPYDAELFGHWWFEGPA
                     WLERIARGFATAGVTPMTLGEALEAVPPRGTVALPEGSWGEGGDHRVWLNPGTEWTWE
                     RVYSAEGEWADLLGRLDGAGDDFRRVLAMATRELLLLEASDWQFLITTWAARDYAEKR
                     VTEHYAEFERLAEMARALAGGEPLSAEGAGMLRRLERQEFCFPDLDPAWGRAVD"
     gene            4697..5938
                     /gene="glgC"
                     /locus_tag="VLF19_10805"
     CDS             4697..5938
                     /gene="glgC"
                     /locus_tag="VLF19_10805"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSE93787.1"
                     /translation="MPRRTRVLTMIMAGGKGERLHPLTRERSKPAVPFGGRHRIVDFV
                     LSNFVNSDLLSLYILVQYKSQSLIEHVRLAWRTSGILPDAFVTIVPPQMRTGPGWYRG
                     TADAVLQNLNLVDDFAPDVIAVFGSDHIYRMDVNQMLAFHLESGAGVTVAARPVPLVE
                     ASQFGVLAVDRQGRVTHFDEKPESPAALPGEPGRALVSMGNYLFNRAVLTDALLADAR
                     RSTEHDFGRTIIPELVPGVPVYAYDFQQNEVPGVKAYEEPGYWRDVGTIEAYWHAHMD
                     LLGEAPRFDLDNRDWPILTGQQPGPPARFIGSEVDDAHVSEGALIKRATIRHSILGRN
                     VWVNEGAVIEDSIIMDHTTVGKGARLRRAIVDRFNIIPADTEIGFDAAADRRRWHADP
                     SGLVVVPRGGRREFLRGLDDL"
     gene            5938..8334
                     /locus_tag="VLF19_10810"
     CDS             5938..8334
                     /locus_tag="VLF19_10810"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HSE93788.1"
                     /translation="MSDRFVAIHGHFYQPPRENPWLDAVEIQDSAAPYHDWNERITAE
                     CYAPNTAARRVTPDNRILDIVNNFERISFNVGPTLMAWLERHAPEVYGKIVEADRVSA
                     AARGGHGNAIAQAYNHVIMPLATRRDKITQVLWGLADFRARFGREPEGMWLPETAVDN
                     ETLEVLAEAGLGFSILAPHQARRVRPVGAETWQEVKEGIDPSRPYLWQGPRGLRLALF
                     FYDGPISRAIAFGDGLDRGERLVARLEAGFDGSRDGAQLVHCATDGESYGHHKTFGEM
                     ALAAAIQQLEAQDAAVLTNYGAFLAAHPPTHEVEIHERTSWSCVHGIERWRADCGCRV
                     RGDWHQRWRGPLREALDWLRDQVDALYEARASAFLKDPWGARDAYVAVVLDRRPERLA
                     EWLERHQRAPLDAIGQVEVRRLLELQRNRLLMYTSCGWFFDEISAIESVQILRYAALA
                     LQYVRDLGGGSLEPELVRRLEAAPSNVADFGDGAEVYRRLVRPAAADLRRVVAHYAIT
                     GLVDEQPDETDVYAWRITRLDEGRESYHGTTVRIGHVRVAARMTGEVREAMYALLHFG
                     GHDFSCGIRGWEDPQTYEAMKADLLGRYEQHSLADMVRGLDEYFPRELCSLTHLFIEE
                     RRRVLASVIAAVLDGHEETYHHVWEESRKLVRYLRQAEAPIPDVLRITAKHVLESEVV
                     RALERTEETLALPERAFELADEARALGVTLDLAAARPLMMRAVGAALEAVRARPAPDR
                     VAAATALITGAQRVGLRFGLWRTQNRFFTIWRERPAARPALARLAAVLGFDLPLEPAP
                     "
     gene            8331..11159
                     /gene="treY"
                     /locus_tag="VLF19_10815"
     CDS             8331..11159
                     /gene="treY"
                     /locus_tag="VLF19_10815"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HSE93789.1"
                     /translation="MTAAPPPRPLRATYRLQLGPDCTFDEAARLTRYLAALGISDCYT
                     SPFFEACAPGSHGYDVCDHNRLDEALGGEAGFRRLADALRAHGLGLVIDVVPNHMGIA
                     GNRNAWWNDVLEHGPASPFARVFDIEWVPVKPELAGKVLLPILGDHYGATLESGQLRL
                     TLADGVFTIRYHDRVLPVAPRSYGRILGHRIDDLQATLGPDHPALLELKTLIAWFVTL
                     PPRDERDPERLAARRRDRQRGVERLAALLRESPEIRAFVDDNVARLNGTPGDPASFDL
                     LDDLLDDQAYRLAYWRVAGEEINYRRFFDVNELAAIRMEESPVFADTHALIVRLVAEA
                     PVTALRIDHPDGLYAPTEYFRRLRAAVGRPVAILAEKVLAPGERLPAAWPVEGTTGYE
                     FLNLLNGIFVDRSQARAMEQIYARLIKERPPFAEVVYASKRLIMDTSMAAELNMLAHR
                     LNGISEKHRSSRDFTLMSLMRALSEIVANFPVYRTYVGDSPAGPSEADREYIARAVGN
                     AKRRTPTLNVSIYDWIQDILTLRHPAWATDADRQARLDFAMRFQQITAPVTAKGYEDT
                     ALYRFARLISLNEVGGDPNRFGASLAEFHAAMAERQRQHPGGLSTTATHDTKRGEDVR
                     ARIDVLSEIPREWRARVGVWQRLNRHHRTAVDGRPVPGPNTEYFIYQTLIGAWPITVD
                     RLRPYLSKAVREAKSHTSWTNPNPRYDEAIARFAEALLDPERSRAFLDDFTPFQARVA
                     RYGMLNSLAQTLVKITTPGVPDFYQGTELWDLSLVDPDNRRPVDFDVRERLLASLADA
                     VEGRPDLAGLAADLWAKPEDGRVKLFVIRQALACRRARAELFAGGEYRPLEVEGELTE
                     HVCAFARVAAGDAVVTLVPRLLARRQGGPPLGMPYWGETRVRVPAAVGTRFHNVLTGE
                     QVETSDEAVAVGRALRSFPVALLEREAG"
     gene            11159..11827
                     /locus_tag="VLF19_10820"
     CDS             11159..11827
                     /locus_tag="VLF19_10820"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HSE93790.1"
                     /translation="MRTAEHPFVFIGCVELRQALDRQALDERELMDRLEEVPVGSVFY
                     HTHGYFLRHRPLTTAWGNDFARWVAVEVRDLALAERLAVVDPFEFRGLDDLREELMTI
                     IHDHLRRLPAVPRADEGAVFHFQQSHIVQVVLGPRASTLAEFRDALATVDASAIYFHM
                     VEARARLGRRSGDFAEWLHGSLGLPALADRIERIDTYMTSLERVRARVLSIVEEALET
                     QDAR"
     gene            11925..>12702
                     /locus_tag="VLF19_10825"
     CDS             11925..>12702
                     /locus_tag="VLF19_10825"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HSE93791.1"
                     /translation="MSPRLDDYRQVAPPGAVDIVLKLAERVRGRRFLHVTGGRFGGGP
                     TEVLAAAVPILNDIGVDTRWEITGGDPAFYATSRALHATLEGAERVITDEALGHWTEM
                     NRVNAKKLDLRADLVLVHDVPPAGLVADRAEGRWLWRCHFDASRALRRGWSFFRRSVD
                     QYDAAIFSLPEFGRRLGVPQYVIHPSIDPLSDKNRELSPREVASIAGALGVARDKPLL
                     LQVGPFTQRQDPLGVVNAYRLVKKHHDVRVVLAGTAGDDRE"
BASE COUNT         1802 a         4691 c         4494 g         1715 t
ORIGIN      
        1 gtgatgccgc cggggatcgc gcgccgggcg cgctcggcga gcgcggccga cttcgggtgc
       61 gcggcccggt accgctcgtg gatcgtcatg acggcctcat cataccgcga ggcgcagcag
      121 ctcgacgaag gccgcgtgaa cctcgagcgg gagaccgtcc accgtgggct ccgcacgacg
      181 gacgagcccg tccacggcac ccgccttcac gcacgcctcg acgagccgcc gctcgtcggc
      241 cggtgtgtgg agcagcgggc ggcgcgccag gcgcgcgagc cccgccacga tcccgtaggc
      301 gccccagttc gacgtcccgg cgacgacgag atggtccacg cgcacgacgg acgaaatctt
      361 cgccatgagc gggcccatcc gcgccaggcg ggcacggaca ttgcccatgc cgatctcgtt
      421 gccaccatcg ccgacggcga tggtgatcgg cccggtcttc cccacgcgca agaacagctc
      481 atccatggga ctgttccacg gcgcgaccga ctcgccgcgc gcgctcaggt agtcgcccga
      541 gcgcgcgcgc ccggggcgct cgacggccac gagatgcgtg ggccgctcgc gcgccaggag
      601 cgccctggcg tcaccgtcga acacctcgac ctccgccggc tccccgaggg catcgagcgc
      661 ggcggcgagc ggcgccacca cgggagcgtc ggtgacatag cgcactcggg ccccgagccg
      721 gcggagcgcg cggccgagga cggcggcccc gggtgggccg tcggtctccg acaggccggg
      781 cggcacgcag aagccggtga cgacgagcac gcggcgggcg gagcggagtg tgcacgccgc
      841 ggcacccgcc gcgcctggaa cgaagaacga ggggatgccg cgggcgccgg gatcgagcgc
      901 caggagatgg tcgacggcgt cgaagcgggg gtttccgcgc cgcatcggcg cgaccctatc
      961 acccgctcag cgcgtagagc aacacctgcc acggctcgag cgggcgcacg cgctccggtc
     1021 ctccgaagcg cgcctccgcg ctgtcgagga gcacccgcga tcgcggcggc gccggccaga
     1081 tctggggctt ggcgccgagg ttcgccacgc actgcacggc ctccccggac gggccggcgc
     1141 ggtccaccac gagcacgctc ccggtctcgt cgagggcggc ccgtgtccgc gacttgccgc
     1201 gcgcgcccag cgcggggtgc tggcgccgca gggcgagcca ggcgcgatag aactcgcgga
     1261 gcgcgcggtg acgaggcgcg ccggcgagcg cgtgattgag ccgggagcgc acgtaagtgt
     1321 ccggcgcctg agggtcggaa atggcgcccg tccacccgaa gcggctgaac tcggcggcgc
     1381 ggccccgacg gacggcagcg gccagcccgg ggtcgaggaa cgacgtgaag aactggaacg
     1441 gcgcggtctc gccgtactcc tcgcccatga agaggagtgg cagggccggc gccaccatga
     1501 gcagcgcggc ggcgagcttc gccgcttcga acggcacgat ggtggcgagg cgatcgccct
     1561 gcgcgcggtt gcccacctga tcgtggttct gggtgcagag gacgaagcgc tcgccctcga
     1621 cgtcgccact cggcgtgccg cgcggtcgcc cgaagtagac cgacggctcg ccctgatagg
     1681 cgaagccctc ggcgagcgcg cgcggcaggt gctgaccgtc ggcgaagtcg acgtagtagc
     1741 cggtggtctc gccggtgagc cgcacgtgca gcgcgtgatg gaagtcgtcg gcccacaccg
     1801 cgtcgaggcc gatgccgccg gcctcggctg gcgtcacgat cgcccggtcg ttgtcgtgcg
     1861 actccgccgt gacgtggccg gggcggccga gactcgcggc ctcctcgcgc acggccgccg
     1921 cgatctcgat gaggatgtgc accgggctcg agtcgaagat ggagtggatg gcgtccagcc
     1981 gcagcccgtc cacgtggaac tcgcgcaccc acatgcgcgc gttctccacg aagtgacggc
     2041 gcacgccggc ggcgtcgggg ccgtcgaagt tcaccgcctg gccccagggc gtctcgtagc
     2101 ggtcggtgaa gtaaggcccg tattccgcca ggtagttgcc ctcgggcccc aggtgattgt
     2161 agacgacgtc caggaagacg ctcagaccgc gcgcgtggca ggcgtccacc agccgcgaga
     2221 gcccccgcgg ccccccgtac gtggactgcg gcgcgaagag gtgcacgccg tcgtagcccc
     2281 agctgcggga ccccgggaac tcggcgatcg gcatcagctc gacggccgtc acgccgagct
     2341 cggcgagccg gtcgaggtac gggatcacgc cctcgaaggt gcccgccgtc gagaaggtcc
     2401 cgacgtgcag ctcgtagaag accaggtcgg cgagggcgtg gccccggaag cgctcgtcgg
     2461 tccacgcgaa cgcggcggta tcgacgacga ccgacgggcc gtgcacgccc tcgggctgcc
     2521 agcgcgagac cggatcaggt cggtaccggc gcccgtcgag ccggtactgg taccgcgcgc
     2581 ccgcccggac gcgcttgaca cccagctcga agaggccctc gcccacggcc gtcagcggct
     2641 gcggcggtcc gccgtccagg aggacatcca cggtccggca ccggggcgcc cagacgcgaa
     2701 aggtggcgcc accctcatga atgcttgcgc ccggtccgtt catacggtcc aatgggagcc
     2761 ccctcgaacc cggacgcggg ctcgccgggg gtgacaaaag gcgcgcgcgc atgagctatc
     2821 cattctgcct cgtccttcac acccatcttc cgatggtggt caatcacggt cgctggccgc
     2881 acggcagcga ctggctctgc gaggccacct tcgagtgcta tctgcccctg ctcgacaccg
     2941 tgcaccggct tctcgccgac ggcatcaagg ccaagtggac gatcaacgtc tcccccgtcc
     3001 tcaccgagca gctcgcgtcg ccggagttcc agcgggagct ctctttctac tacgagaacg
     3061 tgcggcgggc ctgtgccgac agccgccagc acttcggccg cgagggcaac caccagatcg
     3121 tcaagctgac ccacttctgg gaggaattct acgagcgcat gtgggagctg caccggcgga
     3181 tcggcggcga catcccgggc acgctcgcgg atctcgagcg tcagggcgcg atcgaggtca
     3241 tcacctgcgc ggccacccac ggctatctgc cgctgctctc acgcgacgag tcgatccgcc
     3301 tgcagctgag gaccgcggtc gagacccacc gccgtcacta tggccggcgc ccgcgcggca
     3361 tctggcttcc cgagtgcgcc taccggccgc gctacgcgtg gacgccgccg acgggacggg
     3421 agcaggacaa gacgcggcgc atgcggccgg gcctcgagga gatgctggcc gccaacgggc
     3481 tgcagtactt cgtcgccgac tcccacctca tcgccgcctc cgagcccgtc ttcctctatc
     3541 gcgactacgt gccgctgcgg ggccagctcg gcgacccggt ggccgaggtg ccgcgcacga
     3601 atggtcgcac accctacgcg ccctggcggg tcgcctcgcg gggcggcacg ggctcggccg
     3661 tcgccttctt ccgcgatccc aagacgacgt tgcaggtctg gagtcgcgcg cacggctacc
     3721 cgggggactt ccactacctc gagttccaca agaagcacca ccccggcggt ctccgcttct
     3781 ggcgtatcag cgacgccgcc aacgatctcg gctcgaagca gccgtacgat cccggcgcgg
     3841 ccatggagcg ggtggagtgg caggcgcgcc acttcctcga actggtgcgc gacacggcgc
     3901 aggccgccgg cggcgacggg cccgcgctcg tgtgctcgcc gtacgacgcc gagctgttcg
     3961 ggcactggtg gttcgagggc ccggcgtggc tcgagcgcat cgcccggggc ttcgccacgg
     4021 ccggcgtgac tccgatgaca ctgggcgagg ccctcgaggc ggtgccgccc cgcgggacgg
     4081 tagcgctgcc ggagggttcg tggggtgagg gcggcgacca ccgcgtgtgg ctgaatccgg
     4141 gcaccgagtg gacgtgggag cgcgtctaca gcgccgaggg cgagtgggcg gacctgctcg
     4201 ggcggctcga cggcgccggc gacgacttcc gccgggtact cgcgatggcc acgcgcgagc
     4261 tgctgctgct cgaggcctcg gactggcagt tcctgatcac cacgtgggcg gcgcgcgact
     4321 acgccgagaa gcgcgtcacc gagcactacg cggagttcga gcgcctggcc gaaatggccc
     4381 gggcgctcgc cggcggcgag ccgctctcgg ccgagggtgc cggcatgctg cggcggctgg
     4441 agcggcagga gttctgcttc cccgacctgg accccgcatg gggccgcgcc gtagactgag
     4501 ctcgtgaacg agctgcggag tcgggggggg ccacggtggc gccggggcgc cccgagcgtt
     4561 ggggggcatg ggggacgccc gtcagacgcg acacacaacc accgtgcgat ctcgaggagg
     4621 gccacggctg cgccggggac ctccgagcgc cggggggctt ggggggccct ttcgaggccc
     4681 cccatgtact agatccgtgc cccgccgcac gcgcgtcctg acgatgatca tggccggcgg
     4741 caagggtgag cgcctgcacc cgctcacccg cgaacggtcc aagcccgccg tgccgttcgg
     4801 aggccgccac cgcatcgtcg acttcgtcct gtcgaacttc gtcaactcgg acctgctctc
     4861 cctctacatc ctggtccagt acaagtccca gtcgctgatc gagcacgtgc gtctggcctg
     4921 gcgcacgagc ggcatcctgc cagacgcgtt cgtgaccatc gtgccgccgc agatgcgcac
     4981 gggcccgggg tggtaccgcg gcaccgccga cgccgtgctc cagaacctga acctcgtcga
     5041 cgacttcgcc cccgacgtga tcgcggtgtt cgggtccgat cacatctacc ggatggacgt
     5101 caaccagatg ctcgcgttcc acctggaatc gggcgccggc gtcaccgtcg ccgcgcgacc
     5161 cgtcccgctc gtcgaggcct cgcagttcgg cgtgctcgcc gtggaccgac agggccgcgt
     5221 gacgcacttc gacgagaagc ccgagtcgcc ggcggcgttg cccggggaac ccgggcgcgc
     5281 gctcgtctcg atgggcaact acctcttcaa ccgcgcggtc ctcaccgacg cgctgttggc
     5341 cgacgcccgg cgctccaccg agcacgactt cggccgaacc atcatcccgg agctggtgcc
     5401 gggtgtcccc gtgtacgcct acgacttcca gcagaacgag gtgccgggcg tgaaggccta
     5461 cgaggagccc ggatactggc gcgacgtggg cacgatcgag gcctactggc acgcgcacat
     5521 ggatctcctg ggcgaggcgc cgcgcttcga cctcgacaac cgcgactggc cgatcctcac
     5581 cggccagcag cccggcccgc cggcgcgctt catcggcagc gaggtggacg acgctcacgt
     5641 cagcgagggg gcgctcatca agcgggcgac catccgccac tcgatcctcg gccggaacgt
     5701 gtgggtcaac gagggcgcgg tgatcgagga ttccatcatc atggaccaca ccacggtggg
     5761 caagggggcc cggctgcgcc gggccatcgt ggaccgcttc aacatcatcc cggccgacac
     5821 agagatcggg ttcgacgccg cggccgatcg gcgccggtgg cacgccgacc cctcggggct
     5881 ggtcgtcgtg ccgcgcggcg gccggcggga gttcctgcga gggctggacg acctctgatg
     5941 tccgaccgct tcgtcgccat ccacgggcac ttctaccagc cgccccgcga gaacccgtgg
     6001 ctcgacgccg tggaaataca ggactcggcg gccccctacc acgactggaa cgagcgcatc
     6061 acggcggagt gctacgcgcc caacaccgcc gcgcgccggg tgacccccga caaccgcatc
     6121 ctcgacatcg tcaacaactt cgagaggatc tcgttcaacg tcggcccgac gctgatggcc
     6181 tggctcgagc gtcacgcgcc cgaggtgtac ggcaagatcg tggaggccga tcgggttagc
     6241 gccgctgcgc gcggcggcca cggcaacgcg atcgcgcagg cctacaatca cgtgatcatg
     6301 ccgctggcca cgcggcggga caagatcact caggtcctct gggggctcgc cgacttccgc
     6361 gcacgcttcg ggcgcgagcc ggaaggcatg tggctgcccg agaccgccgt cgacaacgag
     6421 acgctcgagg tgctggccga ggcaggtctg ggcttcagca tcctcgcccc ccaccaggcg
     6481 cggcgcgtgc ggccggtggg cgcggagacc tggcaggagg tgaaggaggg catcgacccg
     6541 agccggccgt acctctggca gggaccgcgc gggctccgcc tggcgctgtt tttctatgac
     6601 ggcccgatct cgcgcgcgat cgccttcggc gacgggctcg accgcggcga gcgcctggtg
     6661 gcccggctcg aggcgggctt cgacgggagc cgcgacggcg cgcagctggt ccactgtgcc
     6721 accgacggcg aatcgtacgg ccaccacaag accttcggcg agatggcgct ggccgcggcc
     6781 atccagcagc tcgaggcgca ggacgccgcc gtcctcacga actacggcgc cttcctcgcc
     6841 gcccatccgc cgacgcacga ggtggagatc cacgagcgca cgtcgtggtc gtgcgtgcac
     6901 ggcatcgagc ggtggcgggc ggactgcggc tgccgcgtcc ggggcgactg gcaccagcgc
     6961 tggcgcgggc cgctccgcga ggccctggac tggctgcgcg accaggtgga cgcgctctac
     7021 gaggcgcgcg ccagcgcctt cctcaaggac ccctgggggg cgcgcgacgc ctacgtggcc
     7081 gtcgtgctcg accgccgccc cgagcggctg gccgagtggc tggagcgcca ccagcgcgcg
     7141 ccgctcgacg ccatcgggca ggtcgaggtg cggcgcctgc tcgagctgca gcgcaaccgg
     7201 ctgctcatgt acacctcgtg cggctggttc ttcgacgaga tctccgcgat cgagtccgtg
     7261 cagatcctcc ggtacgccgc gctggccctg cagtacgtgc gggacctggg cggcggcagc
     7321 ctggaacccg agctcgtgcg ccggctcgag gccgccccca gcaacgtcgc cgacttcggc
     7381 gacggcgccg aggtctaccg gcggctggtg cggccggcgg ccgccgacct ccggcgcgtc
     7441 gtcgcgcact acgccatcac gggcctcgtc gacgagcagc ccgacgagac cgacgtctac
     7501 gcgtggcgga tcacgcgcct ggacgagggg cgcgagtcgt accacggcac cacggtccgc
     7561 atcggccacg tgcgcgtcgc cgcgcggatg acgggagagg tgcgggaggc gatgtacgcg
     7621 ctcctgcact tcggcggcca cgacttctcg tgcggcatcc gcggctggga ggacccgcag
     7681 acctatgagg cgatgaaggc cgacctgctc gggcggtacg aacagcacag cctcgccgac
     7741 atggtccgcg gactcgacga gtactttccg cgcgagctct gctcgctcac ccacctgttc
     7801 atcgaggaac gccggcgcgt actggccagc gtgatcgctg ccgtgctcga cgggcacgag
     7861 gagacctacc atcacgtctg ggaagagagc cgcaagctcg tccggtacct gcggcaggcc
     7921 gaggctccca tcccggacgt cctccgcatc accgccaagc acgtgctgga gtcggaggtg
     7981 gtccgcgcgc tcgagcgcac ggaggagacg ctcgcactgc ccgagcgcgc attcgagctg
     8041 gccgacgagg cccgcgcgct cggcgtgacc ctggacctcg ccgccgcgcg gcctctgatg
     8101 atgcgggccg ttggcgcggc cctcgaggcc gtccgggcgc ggccggcgcc cgatcgtgtc
     8161 gccgccgcca cggcgctgat cacgggggcg cagcgggtcg gcctgcgctt cggcctctgg
     8221 cggacccaga accgcttctt caccatctgg cgcgagcggc ccgcggcgcg gccggcgctg
     8281 gcgcggctgg ccgcggtgct cggcttcgac ctgccgctgg agccggcgcc gtgaccgcgg
     8341 ccccgccacc gcgccccctg cgggcgacct accgcctgca gctcggcccc gactgcacct
     8401 tcgacgaggc cgcgcgcctg acgcgctacc tggcagcgct cggcatctcg gactgctaca
     8461 cctcgccctt cttcgaggcc tgcgcgcccg gctcccacgg ctacgacgtg tgcgaccaca
     8521 accgcctcga cgaggcgctg ggcggcgagg ccgggttccg ccgcctcgcc gacgcgctgc
     8581 gagcccacgg gctggggctc gtcatcgacg tcgttccgaa ccacatgggc atcgccggca
     8641 accgcaacgc gtggtggaac gacgtcctcg agcacggccc ggcctcgccg ttcgcacggg
     8701 tgttcgacat cgagtgggtg ccggtcaagc ccgagctcgc cggcaaggtg ctgttgccga
     8761 tcctcggcga ccactacggc gccacgctgg agagcggtca gctgcgcctc acgctggccg
     8821 acggcgtctt cacgatccgg taccacgacc gcgtgctgcc ggtggccccg cgcagctacg
     8881 gccggatcct cggccaccgc atcgacgacc tgcaggcgac gctcgggccg gaccacccgg
     8941 ccctgctcga gctgaagacg ctcatcgcct ggttcgtcac gctgccgccg cgcgacgagc
     9001 gcgaccccga gcggctcgcc gcccgccggc gcgaccggca gcggggcgtc gagcggctgg
     9061 cggccctgct ccgcgagtcg cccgagatcc gggcgttcgt ggacgataac gtggcccgcc
     9121 tgaacggcac gccgggcgac ccggcgtcgt tcgacctgct cgacgacctc ctcgacgacc
     9181 aggcgtaccg cctggcctac tggcgcgtcg ccggcgagga gatcaactac cgtcgcttct
     9241 tcgacgtcaa cgagctggcg gcgatccgca tggaggaatc cccggtcttc gccgacacgc
     9301 acgcgctcat cgtccgcctg gttgccgagg cgccggtgac cgcgctgcgc atcgaccacc
     9361 ccgacggcct ctacgcgccg acggagtact tccgccggct tcgggcggcc gtgggccgcc
     9421 ccgtggccat tctcgccgag aaggtcctgg ccccgggcga gcggctgccc gccgcctggc
     9481 ccgtggaggg caccacgggc tacgagttcc tcaacctcct gaacggcatc ttcgtcgacc
     9541 gcagccaggc gcgcgcgatg gagcagatct atgcgcggct gatcaaggag cgcccgccgt
     9601 tcgccgaggt cgtctacgcg tccaagcgcc tcatcatgga cacgtcgatg gccgcggagc
     9661 tcaacatgct cgcgcaccgg ctgaacggca tctccgagaa gcaccgctcc tcgagggact
     9721 tcacgctgat gagcctcatg cgcgcgctga gcgagatcgt cgccaacttc cccgtctatc
     9781 gcacgtacgt cggcgacagc ccggcgggcc cgagcgaggc cgaccgcgag tacatcgcgc
     9841 gtgcggtcgg caacgccaag cgtcgcacgc cgacgctcaa cgtgtcgatc tacgactgga
     9901 tccaggacat cctcaccctt cgccatccgg cgtgggcgac cgacgcggat cggcaggcgc
     9961 gcctggactt cgccatgcgg ttccagcaga tcaccgcgcc ggtcacggcc aagggttacg
    10021 aggacaccgc gctctaccgc ttcgcccggc tcatctccct caacgaggtg ggcggtgatc
    10081 ccaaccgctt cggcgcctcg ctcgcggagt tccacgccgc gatggccgag cgccagcggc
    10141 agcaccccgg ggggctcagc acgacggcga cgcacgacac caagcgcggc gaggacgtgc
    10201 gcgcgcgcat cgacgtgctg tccgagatcc cgcgcgagtg gcgggcgcgc gtgggggtgt
    10261 ggcagcggct gaaccggcac catcgcacgg ccgttgacgg tcggcccgtg cccgggccca
    10321 acaccgagta cttcatctat cagacgctga tcggggcctg gcccatcacg gtggaccggc
    10381 tgcgtccgta tctctcgaag gccgtccgcg aggccaagtc gcacacctcg tggacgaacc
    10441 ccaacccgcg ctacgacgag gcgatcgcgc gcttcgccga ggcgctcctg gaccccgagc
    10501 gctcgcgcgc cttcctcgac gacttcacgc cgttccaggc gcgcgtggcg cggtacggca
    10561 tgctcaactc gctggcgcag acgctcgtca agatcacgac gccgggcgtg ccggacttct
    10621 accaggggac cgagctgtgg gacctctcgc tggtcgaccc cgacaaccgc cgtcccgtgg
    10681 acttcgacgt gcgggagcgg ctgctcgcca gcctcgccga cgccgtggag gggcggccgg
    10741 acctggccgg gctcgccgcc gacctctggg ccaagcccga ggacgggcgc gtcaagctct
    10801 tcgtgatccg tcaggcgctg gcgtgccggc gcgcgcgcgc cgagctcttc gccggcggcg
    10861 agtaccggcc gctggaggtc gagggcgagc tgacggagca cgtgtgcgcg ttcgcgcgcg
    10921 tggccgccgg cgacgccgtg gtcacgctcg tgccgcggct gctcgcgcgc cggcaggggg
    10981 gcccgccgct cggcatgccc tactggggcg agacgcgagt gcgcgtgccc gccgccgtgg
    11041 gcacgcgctt ccacaacgtg ctgaccggcg agcaggtgga gacctcggac gaggcggtgg
    11101 ccgtgggccg cgcgctccgg agcttcccgg tcgcgctcct cgagcgcgag gccggctgat
    11161 gcgcaccgcc gagcatccct tcgtcttcat cgggtgcgtc gagctgcgcc aggcgctcga
    11221 ccggcaggcg ctcgacgagc gcgagctgat ggaccggctg gaggaggtgc ccgtcggctc
    11281 ggtgttctac cacacgcacg ggtacttcct ccgtcaccgg ccgctgacca ccgcgtgggg
    11341 caatgacttc gcccgctggg tggccgtcga ggtgcgcgac ctcgccctgg ccgagcgcct
    11401 ggccgtggtg gaccccttcg agttccgcgg gctcgacgac ctgcgcgagg aactgatgac
    11461 catcatccac gaccacctgc gacggctgcc ggcggtgccg cgcgcggacg agggcgcggt
    11521 gttccacttc cagcagtcgc acatcgtgca ggtcgtgctg ggtccccggg ccagcacgct
    11581 cgccgagttc cgcgacgcgc tggccacggt ggacgccagc gccatctact tccacatggt
    11641 cgaggcgcgc gccaggctcg ggcgccgatc cggcgacttc gccgaatggc ttcacggctc
    11701 gctcggactg cccgcgctgg ccgaccgcat cgagcgcatc gacacctaca tgacgagcct
    11761 cgagcgcgtg cgcgcccgtg tgctgtccat cgtggaagag gccctcgaga cccaggacgc
    11821 ccgatgacga acagtgcact ccgcgcctcg ttgctcgcct cggcacccgg gcgggcctcg
    11881 gcccgccgtg cgggcagcgg tcacggcccg cggctcgcac gaccgtgagt ccgcgcctgg
    11941 acgactaccg gcaagtcgcg ccgcccggcg cggtcgacat cgtcctcaag ctcgccgagc
    12001 gggtgcgcgg gcgccgcttc ctgcacgtca ccgggggccg gttcggcggc ggcccgaccg
    12061 aggtgctcgc cgccgcggtg ccgatcctga acgacatcgg agtcgacacc cgctgggaga
    12121 tcaccggcgg cgatccggcc ttctacgcca cctcgcgggc cctgcacgcg accctggagg
    12181 gcgccgagcg agtcatcacc gacgaggcgc tcggccactg gaccgagatg aaccgcgtga
    12241 acgccaagaa gctcgacctg cgcgccgacc tcgtgctcgt ccacgacgtg cccccggcgg
    12301 ggctcgtggc ggaccgcgcc gagggacgct ggctctggcg ttgccacttc gatgcctcgc
    12361 gcgcgctgcg ccgcggctgg tcgttcttcc ggcgatccgt ggaccagtac gacgccgcga
    12421 tcttctcgct gcccgagttc gggcggcgcc tcggcgtgcc gcaatacgtg atccacccgt
    12481 cgatcgatcc gctgtcggac aagaaccgcg agctgtcgcc gcgcgaggtg gcgtccatcg
    12541 ccggcgcgct cggcgtggcc cgcgacaagc ccctgctgct ccaggtcggg cccttcacgc
    12601 aacggcagga cccgctcggc gtcgtcaacg cgtaccgtct cgtcaagaag caccacgacg
    12661 tccgcgtggt gctcgcgggc acggccggcg acgaccggga ga
//
LOCUS       DATIAZ010000309         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1405180, whole genome shotgun sequence.
ACCESSION   DATIAZ010000309 DATIAZ010000000
VERSION     DATIAZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405180"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1734)
                     /locus_tag="VLF19_10830"
     CDS             complement(169..1734)
                     /locus_tag="VLF19_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HSE93792.1"
                     /translation="MSGAALAEENARLKALLAQTQSALAESEEARRRLEAILRDLQRE
                     KFGAKSEKLSPDQFSLALEDVELAQGILEAAQEKAAAILKGKTGGDTRSRHRNRGQLP
                     AHLPRIERIIEPESTLCPCGCGEMTKIGEDVSERLDVVPAQLRVLVTRRPKYACRRCA
                     GAVAQAHAPEHVVPSGLPTEALIAEVIVAKFGDHLPFYRQAEIYARQGIKLDRATLGN
                     WAGRACFHLQPVAEHMRRHLAGAERLFMDETTAPVLDPGRGQTKKGFFWAIAADDRGH
                     GGPSPPVVLFRYAPGRGGIHAERFLEGFRGRFLQCDGYDGYECLTGVERPEGSWTLVH
                     CWSHLRRRFVKQMRSTKSPIAEAAVRQIATLYAIEASVRGMAPAPRLAARQAQSAPIV
                     AALKTWFEKQLSMISSGSTLAADIRYALAHWPGLIRYLEDGRLEMDTNPVENVIRPVA
                     LTRKNALFAGHEVGAENWALLASIVATCKLNDVNPVHYIADTLEAILEGHLQSRIEEL
                     MPWRYQKPSSLAA"
     gene            complement(1803..2156)
                     /gene="tnpB"
                     /locus_tag="VLF19_10835"
     CDS             complement(1803..2156)
                     /gene="tnpB"
                     /locus_tag="VLF19_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491906.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HSE93793.1"
                     /translation="MIAPGGPLRVYVATRPVDFRKGHDGLAAVVQEMFGLDPFCGAAF
                     VFRSKRADRIKVLVWDRTGMVLVHKRLEGGKFVWPQVRDGVMKMSPAQFSALFEGLDW
                     RLMRPERARRPQLAG"
     gene            complement(2153..2578)
                     /locus_tag="VLF19_10840"
     CDS             complement(2153..2578)
                     /locus_tag="VLF19_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSE93794.1"
                     /translation="MKRDGKMDVHLDVSRDGYVGRLEVIEGPTGRRRRSDAEKARIVA
                     ESLVPGVKVADVARRHGTTRWQVYDWRRRARKGRLAPSAETTPSAAFAAVVVEEAQPG
                     PAVRRSGIEVVVGDVVIRAAPDAAEGHLARVIRAARAAR"
BASE COUNT          413 a         1015 c          912 g          419 t
ORIGIN      
        1 agccgatccg cccagctccg cgaggccacg cggatcgtaa cgatcgcgac gcggctgcgc
       61 gatgacggct ggcgtgaggt ctcggtccgg gacgggccgc tatctaaacc atcacaccga
      121 ttcggcggga gaatctcgcc caactgctgt aagcgctctg ccgatcccct acgctgcgag
      181 gcttgacggc ttctggtagc gccaaggcat gagctcttcg atccggctct ggagatggcc
      241 ctcgaggatg gcctccaggg tgtcggcgat gtagtggacg ggattgacgt cgttgagctt
      301 gcaggtggcg acgatggagg cgaggagcgc ccagttctcg gcgccgacct cgtggcccgc
      361 gaacaacgcg ttctttcgcg tgagagccac cggcctgatg acgttctcga ccgggttagt
      421 gtccatctcg aggcggccgt cctcgaggta tctgatgaga ccgggccagt gggcgagggc
      481 gtagcggatg tccgcggcca gggtggagcc cgaggagatc atcgacagct gcttctcgaa
      541 ccaggtcttc aaggccgcga cgatgggtgc cgactgcgcc tgcctggcgg cgagccgcgg
      601 cgccggcgcc atgccacgga cgctcgcctc gatggcgtag agggtggcga tctgccggac
      661 ggcggcctcg gcgatcgggg acttggtgct gcgcatctgc ttgacgaagc gccgccgcag
      721 gtggctccag cagtgcacca gcgtccagga cccctccggc cgctcgacgc cggtcaggca
      781 ttcgtagccg tcatagccgt cgcattgcag gaaccggccg cggaagccct cgaggaaccg
      841 ctcggcatgg atgccgccac gccccggggc gtagcggaac agcacgaccg gtggactggg
      901 accgccgtga ccgcggtcgt cggcggcgat cgcccagaag aagcccttct tggtctgccc
      961 gcgcccgggg tcgagcaccg gcgcggtggt ctcgtccatg aacaggcgct ccgccccggc
     1021 gagatgccgg cgcatgtgct cggcgaccgg ttgtaggtgg aagcaggccc ggccggccca
     1081 gttgcccaag gtggcccggt cgagcttgat gccctggcgg gcgtagatct cggcctggcg
     1141 gtagaacggc agatggtcgc cgaacttcgc gacgatgacc tcagcgatca gcgcctcggt
     1201 cggcagcccg ctcggcacga catgctcggg cgcgtgcgcc tgcgccaccg ctccggcgca
     1261 gcggcggcag gcatacttcg ggcggcgcgt caccagcacc cgcagctgcg ccggcaccac
     1321 gtcgagccgc tcggagacgt cctcgccgat cttcgtcatc tcgccgcagc cgcaggggca
     1381 gagcgtgctt tccggctcga tgatccgctc gatgcggggc agatgcgccg gcagctggcc
     1441 gcggttgcga tgccgcgaac gcgtatcgcc gccggtcttg cccttgagga tcgcggccgc
     1501 cttctcctgg gcggcttcga gaatgccctg ggcgagctcc acgtcctcga gcgcgaggct
     1561 gaactggtcg gggctcagct tctcggactt cgcgccgaac ttctcgcgct gcaggtcacg
     1621 gaggatcgcc tcgagccggc gccgcgcctc ctcggactcc gccagcgccg actgcgtctg
     1681 cgccaggagc gccttcaggc gcgcgttctc ttcggcaagg gcggctccgc tcatgggcct
     1741 atagaatcag agagattccg gcgccgccag cccgagccga tcccgcaagc ggcctttcgc
     1801 cgtcagccgg caagctgcgg gcgccgcgcc cgctctgggc gcatcagccg ccagtcgagc
     1861 ccctcgaaga gcgccgagaa ttgcgccggc gacatcttca tcaccccgtc gcggacctgc
     1921 ggccacacga acttgccgcc ctccagccgc ttgtggacca gcaccatgcc ggtgcggtcc
     1981 cagaccagca ccttgatcct gtcggcgcgt ttcgaccgga acacgaaggc cgcaccgcag
     2041 aacggatcga gcccgaacat ctcctgcacc accgctgcca gcccgtcatg gcccttccgg
     2101 aaatcgaccg ggcgggtcgc tacatagacc cgcagcggtc cgcccggcgc gatcatcgcg
     2161 ccgctcgcgc cgcccggatc acccgcgcga gatgcccctc cgcggcatcg ggcgccgccc
     2221 ggatcaccac gtcgcccacc accacctcga tgccggacct gcgcaccgcc ggccccggct
     2281 gcgcctcctc gaccaccacc gccgcgaagg cggccgacgg cgtcgtctcc gccgacggcg
     2341 caagccggcc cttccgcgcc cgccgccgcc agtcatatac ctgccagcgc gtcgtcccgt
     2401 gccgccgggc cacgtcggca accttcacgc ccggcaccag gctctccgcc acgatccgcg
     2461 ccttctccgc atcgcttcgc cgccgtcggc cggtcgggcc ctcgatcacc tcgagccgac
     2521 cgacgtagcc gtcccgcgag acgtccaaat ggacgtccat tttgccgtct ctcttcatgc
     2581 cgaccctccg cgcaaatcga aggcgtcgtc gcacgcttag gtcacgcgcg ggagaagggg
     2641 atcggatgag cgcttaccga tggcccatac gggagcaatc tgaagacgag ccactacacg
     2701 gcgacaggtc cccgcgtcat ccggctcggc aacatctgta ccgcacccag tcccttgga
//
LOCUS       DATIAZ010000310         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_746584, whole genome shotgun sequence.
ACCESSION   DATIAZ010000310 DATIAZ010000000
VERSION     DATIAZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746584"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1377
                     /locus_tag="VLF19_10845"
     CDS             301..1377
                     /locus_tag="VLF19_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HSE93795.1"
                     /translation="MARFPVITDAALAELRQRIGRPVRRPEPYIEVATRDAVRHWAHG
                     IGDRNPHWLAHGVAPPTILLAMDRIVSGYVGGLPGIHAMYAGTDFRWERPIRIDDRIV
                     GDSVLLDLVEKPSQFAKRAVQQIYRTTFRNPQGEVVCVADSWCFRTERDTARERGKYR
                     GVARHRYTAAEIEAIKTAYRDEEVRGAAPRWWEEVAVGEALPVVVKGPLTVTSVVAFV
                     QGWGSLYVRGHGLAFDLFERHPALGIPNDFGVPEPPERVHWDEPFAQAVGVPGAYDYG
                     PERVAWLGHLVTSWMGDDAFLRRLSVQVQRHNLIGDTTWCRGEVTGTGSDGVVELSVR
                     GVNQRGEVTASGTATVVLPVRGAR"
     gene            1441..>2241
                     /locus_tag="VLF19_10850"
     CDS             1441..>2241
                     /locus_tag="VLF19_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93796.1"
                     /translation="MTPILAVRSYRAARERFSWDALWSLFDGDRRRLNLAHECVDRHV
                     ARGTALRIQLDDGRREAHDFVALAAWSSRFARFLEREGIERGDRVAIMLEPSLAFYGA
                     LFGAVKRGAVAVPLFTLFGPDGVALRLDDCRPRMLLVEREAAAWQARFPAMRVLAADA
                     HLAERLPHEPAAYEPATAADELAVLQYTSGTTRELPTAVEHTHRSVVTLMIAALYGVG
                     LERGDRFFCPSSPAWGHGLWHGTIAPLALGIPVGAYAGRFDAARLFEAL"
BASE COUNT          271 a          819 c          832 g          319 t
ORIGIN      
        1 cgcggccgat tccgccacga gcgtgatcgt agtcgagccg cgcgggttac gccagagccg
       61 gtgaggcgtt ctctccgtgg gcgtcgccgg gccggcgccc ctcgggcgtc tcgagcccga
      121 ggcattgaca ctgcgcggac gacggtcgtt caatccatgt cgaggaggtg agcgggatgg
      181 cggtggcgct tcaaccctac cggccacgcg ggcagtcgct cgctccagcc gcttttcccg
      241 acctcacgct ccgtgtcgac gacatcctcg gcagcacgcg ggcctgagtg cccccggagc
      301 atggcccggt ttcccgtgat caccgacgcg gcgctggcgg agctgcgcca gcgcatcggc
      361 cggccggtgc gccgccccga gccgtacatc gaggttgcca cgcgcgatgc ggtgcgccac
      421 tgggcgcacg gcatcggcga ccgcaacccg cactggctcg cgcacggcgt ggcgccgccg
      481 accatcctgc tggccatgga ccgcatcgtc tccggctacg tcggcggcct gccggggatc
      541 cacgcgatgt acgcggggac ggacttccgg tgggagcggc cgatcaggat cgacgaccgg
      601 atcgtgggcg acagcgtgct cctcgacctc gtcgagaagc cgtcgcagtt cgcgaagcgc
      661 gccgtgcagc agatctaccg cacgaccttc cggaacccgc agggcgaggt cgtgtgcgtg
      721 gccgactcgt ggtgcttccg caccgagcgc gacacggcgc gcgagcgtgg gaagtaccgc
      781 ggcgtcgcgc gccaccgcta cacggccgcc gagatcgagg cgatcaagac cgcgtaccgc
      841 gacgaggaag tgcgcggcgc cgcgccgcgc tggtgggagg aggtcgccgt cggcgaggcg
      901 ctgcccgtcg tggtgaaggg cccgctgacc gtgacttccg tcgtcgcgtt cgtccagggc
      961 tggggcagcc tctacgtgcg gggccacggc ctggccttcg acctcttcga gcggcatccg
     1021 gcgctcggga tccccaacga cttcggcgtg ccggagccgc cggagcgcgt gcactgggac
     1081 gaacccttcg cgcaggcggt cggcgtgccc ggcgcctacg actacgggcc ggagcgcgtc
     1141 gcgtggctcg ggcacctcgt gacgagctgg atgggcgacg acgccttcct gcggcggctg
     1201 agcgtgcagg tccagcgcca caacctgatc ggcgacacca cgtggtgtcg cggcgaggtc
     1261 accggcacgg gttccgacgg cgtggtggag ctgagcgtgc gcggcgtcaa ccagcgcggg
     1321 gaggtgaccg cgtccgggac ggcgaccgtt gtcctgccgg tccgcggtgc gcggtgagcc
     1381 gcgtgacaca gcgcccggtc cccgcggcgc cgtgagcccg atcctccagg ctgccgccgc
     1441 atgacgccga tcctcgcggt gcgcagctac cgcgcggcgc gcgagcgctt ctcctgggac
     1501 gcgctgtgga gtctcttcga tggtgaccgc cggcggttga acctggccca cgagtgcgtg
     1561 gaccgccacg tcgcgcgggg aaccgccctg cgcatccagc tcgacgacgg ccgccgcgag
     1621 gcgcacgact tcgtggcgct ggcagcctgg tcgtcgcgct tcgcccgctt cctggagcgc
     1681 gagggcatcg agcggggcga ccgcgtggcc atcatgctcg agccgtcgct cgcgttctac
     1741 ggtgccctct tcggggccgt gaagcgcggc gccgtcgccg tgccgctgtt cacgctgttc
     1801 ggcccggacg gcgtggcgct gcggctcgac gactgccggc cgcgcatgct gctggtggag
     1861 cgcgaggcgg ccgcatggca ggcgcgattc ccggcgatgc gggtgctcgc cgccgacgcc
     1921 cacctcgccg agcgcctccc gcacgagccg gccgcctacg agcccgcgac ggcggccgac
     1981 gagctcgccg tcctccagta cacctcgggg acgacgcgcg agctgcccac ggcggtcgag
     2041 cacacccatc gctcggtggt cacgctgatg atcgccgcgc tctacggcgt cggcctcgag
     2101 cgcggcgacc ggttcttctg cccgtcgtcg ccggcgtggg gccacggcct ctggcacgga
     2161 accatcgcgc cgctggcgct gggcatcccg gtcggcgcct acgcgggccg cttcgacgcg
     2221 gcgcgcctgt tcgaggcgct g
//
LOCUS       DATIAZ010000311         4221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_484468, whole genome shotgun sequence.
ACCESSION   DATIAZ010000311 DATIAZ010000000
VERSION     DATIAZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484468"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VLF19_10855"
     CDS             complement(<1..653)
                     /locus_tag="VLF19_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93797.1"
                     /translation="MLRIRDVHAGYGATPILFGVSLEVREGEAVALLGRNGMGKTTLI
                     KTVMGFLAPWRGAIEFDGHALGGRRPHEIARLGVGLVPENRRIFPGLTVRENLELGLS
                     AVRGRPVAVRRERLDAVFRHFPRLAERIDQPGKTLSGGEQQMLAIARVMMAGARIILM
                     DEPTQGLAPALVRHIREMIGELKRLGVTVLLVEQNARMALNVCDRGYIMEKGVIVFEG
                     "
     gene            complement(681..1439)
                     /locus_tag="VLF19_10860"
     CDS             complement(681..1439)
                     /locus_tag="VLF19_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93798.1"
                     /translation="MSSGTVLEVQGLSRSFGALAALANVSFAVRPGEVFSVIGPNGAG
                     KSTLFNVIAGLHVPTAGRVVLRGEDVTGLPPEEINRRGLAKTFQITNIFPEISVFENV
                     RVAAQSRCRESGRLASLWRLPDVDARVLPLLQAFGLAARRDELAENLSHGEQRYLEIC
                     LALATEPTLLLLDEPTAGMTPGETKEATALVRAIARERRLTVLLIEHDMSVVMGISDR
                     VAVLHFGEKIAEGPPEAIRQDPQVVAAYLGGADD"
     gene            complement(1436..2383)
                     /locus_tag="VLF19_10865"
     CDS             complement(1436..2383)
                     /locus_tag="VLF19_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93799.1"
                     /translation="MTREQVPWAALTVFAALALLPLTPWMTKFYMLLAFDALLFGAVA
                     MSLDLLMGYTGLVSFGHAAFFGLGAYATAVLLERGVLSIWLCLGFAVAVVGLYALVVA
                     YFSTTRRGIYFALLTLIFAEVVYTFFRYTQTFGGSDGIQGLPSPQVLPGVALDTPMRH
                     YYTILVYLAVAWGVCRIVVGSHFGRVLVAIRENEDRARFLGYSVQRYKMAVCLISALL
                     TGLGGALYPGRAAYATPDLMLWTVSGEFIIMVMIGGLGTLVGPVIGGVFFVLLQEKVS
                     SYVDWYFIVIGLVLIAIVLFMPRGLFGFRRLLRLGRAPA"
     gene            complement(2385..3263)
                     /locus_tag="VLF19_10870"
     CDS             complement(2385..3263)
                     /locus_tag="VLF19_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93800.1"
                     /translation="MDYLVAVLLPQLLHGLVFGAALGLVALGLTVIFGLLGVMNFAHG
                     ELYMLGAYAGIGVIAITGSFWVALIVAPLLVGVLAVVTEVATLRPLYRREPLYGLILT
                     FGLALVFREGVRQVWGGDMRRILPPVPGSTPVLGMTYPNYRLFLLAVSCLLLLGIWLF
                     FTRTRAGIVVRAAVQDAEMLDGLGVNVPRVFTLTFAGSAALAALAGLLLAPVFTVYPQ
                     MGVELILLAFIVVILGGMGSLGGSVVAAFVIGIAQSLLTLWMNPQRVAIAIFGIMIFV
                     LIVRPRGFFGREGVLE"
     gene            complement(3271..>4221)
                     /locus_tag="VLF19_10875"
     CDS             complement(3271..>4221)
                     /locus_tag="VLF19_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93801.1"
                     /translation="EKLILQDDVKLLVGAVSSGATMAVMDVTKKHKTIHWNSVSCAEF
                     MRTTKFHKYYFSNQPDSRMQANGLAKYIVDKMGKKVYIFYTDYAMGQSDGRQFKTALE
                     KLGGEVVGVAGAPLDTKDFSPWFGAINQSNPDVLFLAFAGTDSLRLMTQLHSFGMTKK
                     YKLAGIDCFLLQQDLPAIAEPMEGFVQLNHFSAYNPDPAMQAFNQKFKKRWNLDANIA
                     AGAYDAVLFWAAAVEKAKTFDSDKVAEAHEGMCLDNTHIGRQCIRKDDHQVIMDMHLY
                     RVEKGKNVPIARIAGSDSIGEPMVGKDPVAGFTWELKKKN"
BASE COUNT          742 a         1380 c         1520 g          579 t
ORIGIN      
        1 ccctcgaaga cgatcacgcc cttctccatg atgtagccgc ggtcgcagac gttgagcgcc
       61 atgcgcgcgt tctgctcgac gagcaggacg gtgacgccga ggcgcttgag ctcgccgatc
      121 atctcgcgga tgtgacgcac gagggccggc gcgagcccct gcgtcggctc gtccatcagg
      181 atgatgcgcg cgccggccat catgacgcgc gcgatggcga gcatctgctg ctcgcctccc
      241 gagagcgtct tgccgggctg gtcgatccgc tcggccaggc gcgggaagtg acggaagacg
      301 gcgtcgaggc gctcgcggcg caccgcgacg gggcggccgc ggacggcgga gaggccgagc
      361 tcgaggttct cgcgcacggt gaggcccggg aagatgcgac ggttctcggg caccagtccc
      421 acgccgagac gcgcgatctc gtggggccgg cggccgccga gcgcgtggcc gtcgaactcg
      481 atggcgccgc gccagggtgc gaggaacccc atgaccgtct tgatcagggt ggtcttgccc
      541 atgccgttcc ggcccagcag cgcgaccgcc tcgccctcgc gcacctcgag ggacacgccg
      601 aagaggatgg gcgtggcgcc gtagcccgcg tggacgtccc ggatgcgcag catcaggtcg
      661 agcgaccgcg cggcatggag tcagtcgtcg gcgccgccga ggtacgcggc gacgacctgc
      721 gggtcctggc ggatcgcctc cggcgggccc tcggcgatct tctcgccgaa gtggagcacg
      781 gcgacgcggt ccgagatgcc catgaccacg ctcatgtcgt gctcgatcag cagcaccgtg
      841 agccggcgct cgcgtgcgat cgcccgcacc agcgcggtgg cctccttcgt ctcgcccggg
      901 gtcatccccg ccgtgggctc gtcgagcagg agcagtgtgg gctcggtggc gagcgcgaga
      961 cagatctcga ggtagcgctg ctcgccgtgc gagaggttct cggccagctc gtcacgccgg
     1021 gcggcgaggc cgaacgcctg gaggagcggc aggacgcgcg cgtccacgtc gggcagccgc
     1081 cagagcgagg ccaggcgccc ggactcgcgg cagcgggact gcgccgccac gcgcacgttc
     1141 tcgaacacgg agatctccgg gaagatgttg gtgatctgga acgtcttggc cagcccgcgg
     1201 cggttgatct cctctggtgg cagcccggtg acgtcctcgc cgcggaggac gacgcggccg
     1261 gccgtgggga cgtggagccc cgcgatcacg ttgaagagcg tggacttgcc ggcgccgttg
     1321 ggtccgatga ccgagaagac ctcgcccggg cgcacggcga agctcacgtt ggcgagcgcg
     1381 gccagggcgc cgaagctgcg cgacaggccc tgcacctcga ggacggtccc ggagctcacg
     1441 ccggcgcgcg tcccagccgc agcagccgcc ggaacccgaa caggccccgc ggcatgaaca
     1501 gcacgatggc gatgaggacc aggccgatga cgatgaagta ccagtcgacg tacgacgaga
     1561 ccttctcctg gaggaggacg aagaacacgc cgccgatgac cgggccgacg agggtgccga
     1621 ggccgccgat catgaccatg atgatgaact caccggacac ggtccacagc atcagatcgg
     1681 gcgtggcgta ggcggcgcgg ccgggataca gggcgccgcc caggcccgtg agcagggcgg
     1741 agatcaagca gaccgccatc ttgtagcgct ggacgctgta accgaggaag cgggcgcggt
     1801 cctcgttctc gcggatggcc accaggacgc ggccgaagtg cgagccgacg acgatgcgac
     1861 agacgcccca ggccacggcg aggtagacga ggatcgtgta gtagtggcgc atcggcgtgt
     1921 ccagggcgac ccccggcagc acctggggcg agggcagccc ctggatgccg tccgagcccc
     1981 cgaaggtctg cgtgtagcgg aagaacgtgt agacgacctc ggcgaagatc agcgtgagca
     2041 gggcgaagta gatgccgcgc cgggtggtgg agaagtaggc caccacgagg gcgtagagcc
     2101 ccaccacggc tacggcgaag cccaggcaga gccagatcga gagcacgcct cgctcgagca
     2161 gcaccgcggt ggcgtaggcg ccgagcccga agaacgcggc gtggccgaac gacacaagcc
     2221 ccgtgtagcc catcaggagg tcgaggctca tggccaccgc gccgaagagc aacgcgtcga
     2281 aggccagcag catgtagaac ttcgtcatcc acggggtcag cgggaggagg gcgagcgcgg
     2341 cgaacacggt gagcgcggcc caggggacct gttcccgcgt catcttactc aaggacaccc
     2401 tcccggccga agaagccgcg gggccgcacg atgagcacga agatcatgat cccgaagatg
     2461 gcgatggcga cccgctgggg gttcatccag agcgtgagca ggctctgggc gatgccgatc
     2521 acgaaggcgg cgacgacgga cccacccagc gagcccatgc cccccaggat gaccacgatg
     2581 aaggcgagca ggatcaattc gacgcccatc tgcggataga cggtgaagac cggcgccagc
     2641 agcagcccgg cgagcgccgc cagggccgcc gagccggcga acgtgagcgt gaacacccgc
     2701 gggacgttga cgccgaggcc gtcgagcatc tcggcatcct gcacggcggc gcgcacgacg
     2761 atccccgccc gggtgcgggt gaagaaaagc cagatgccga gcagcagcag acacgacacg
     2821 gcgagcagga agagccgata gttcggatac gtcatgccca gcacgggcgt cgagccgggg
     2881 accggcggca ggatgcggcg catgtcgccc ccccagacct gacggacccc ctcgcggaac
     2941 acgagggcga ggccgaaggt gaggatgagg ccgtagagcg gctcgcggcg gtagagcggc
     3001 cggagcgtgg cgacctcggt gaccacggcc agcaccccga cgaggagcgg cgcgacgatc
     3061 agggcgaccc agaacgaccc ggtgatcgca atcaccccga tgcccgcgta ggcgcccagc
     3121 atgtacagct cgccgtgggc gaagttcatc acgcccagca gcccgaaaat cacggtcagg
     3181 cccagcgcca ccagccccag cgcggccccg aagaccaggc cgtgcaggag ctggggcagc
     3241 aggacggcga ccagatagtc cacccggcgc ctagttcttc ttcttgagct cccaggtgaa
     3301 gcccgccacc gggtccttgc cgaccatggg ctcgccgatg gagtcgctgc cggcgatgcg
     3361 cgcgatcggg acgttcttgc ccttctccac ccggtacagg tgcatgtcca tgatcacctg
     3421 gtggtcgtcc ttgcggatgc actggcggcc gatatgggtg ttgtccaggc acatgccctc
     3481 gtgggcctcg gccaccttgt cggagtcgaa ggtcttggcc ttctccaccg ccgctgccca
     3541 gaagagcacg gcgtcgtagg cgccggccgc gatgttggcg tcgaggttcc agcgcttctt
     3601 gaacttctgg ttgaaggcct gcatggccgg gtccgggttg taggccgaga agtggttgag
     3661 ctggacgaag ccctccatcg gctcggcgat ggctggcagg tcctgctgga gcaggaagca
     3721 atcgatgccc gccagcttgt acttcttcgt catgccgaag gagtggagct gggtcatgag
     3781 ccgcagggag tcggtcccgg cgaaggcgag gaacaggacg tcggggttgg actggttgat
     3841 cgcgccgaac caggggctga agtccttggt gtccagcggc gcgccggcga cgccgacgac
     3901 ctcgccgccc agcttctcga gcgccgtctt gaactggcgg ccgtcggact gacccatcgc
     3961 gtagtcggtg tagaagatgt agaccttctt gcccatcttg tcgacgatgt acttggccag
     4021 gccgttggcc tgcatccgcg agtcgggctg gttcgagaag tagtacttgt ggaacttcgt
     4081 cgtccgcatg aactccgcgc acgagacgga gttccagtgg atggtcttgt gcttcttggt
     4141 gacgtccatg accgccatcg tggcgccgct cgacacggca ccgacgagga gcttcacgtc
     4201 gtcctggagg atcagcttct c
//
LOCUS       DATIAZ010000312         4294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1142251, whole genome shotgun sequence.
ACCESSION   DATIAZ010000312 DATIAZ010000000
VERSION     DATIAZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142251"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1331)
                     /locus_tag="VLF19_10880"
     CDS             complement(165..1331)
                     /locus_tag="VLF19_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE93802.1"
                     /translation="MTPTDHPTPLDAARKLASQIRSCADETDAERELPRALFEAIADA
                     GLFQMALPRALGCPELDLPTYVQVIEEIGKADASTGWVVNQCAIFATYAARMPRDVAR
                     AIWIDTPRSVVSNTPAPTAQAIVVPGGYRVTGRQGFSTGCRHASWVAAHAQVIENGRI
                     RMDGPEPEARYLFVPRAEAELLDTWKVRGMRGTGTHHFAVHDVFVPAERTVLSATAPL
                     LETGPLYRIPRTLVFASGDASVALGTARTCLAAFYELAGAKTPRAMQALLRDQSMIQV
                     EVGHAEARLRSGRAFLAETVREIWHEATTTGALTLDQRANLRLATTHAIRLAVQVVDA
                     VYHAAGATAAYEGHPIQRHFQDIHVISQHLQSRLSHYELVGRYWLGLKVDETRL"
     gene            complement(1351..2898)
                     /locus_tag="VLF19_10885"
     CDS             complement(1351..2898)
                     /locus_tag="VLF19_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSE93803.1"
                     /translation="MTWEPELDELKRREALAEQLGGPERVRRQHDGGRLTIRERIDAL
                     VDPGSFHEIGKIAGKATYDEHNDLESFTPSNFVFGRARVEGRPVVVGGDDFTVRGGSA
                     DATLKDKHLLCERMANELRLPLIRLVEGSGGGGSVKTIETTGRANVPGVSGWEMVVDN
                     IATVPRVALGLGSVAGLGAAHLAAAHYSVMVRELSALFVAGPPVVARLGQKLSKNELG
                     GWEIQLKAGAVDDAVATEDEAFARARRFLSYLPASIDELPPRGPRTDDPERREPWLVD
                     AIPRDVRKPYKMRVIVEALVDCGSFFEMAPLYGRSIVTGFARLDGWPVALMASDPFFY
                     GGAWGADTCQKVERFVDVAQTFHLPVVYLVDCPGFLIGLDAERTGTIKQGVRAMAAMW
                     QTTVPWCAVIVRNCFGVAGAAHRNGGRFATRYAWPSGRWGSLPLEGGIEAAYRAEIDA
                     AADPKARMAEIETRLTKLRSPFRSAETFWIEEIIDPRETRPLLCDFANLAAPLRRPGP
                     SAHGMRP"
     gene            3024..>4294
                     /locus_tag="VLF19_10890"
     CDS             3024..>4294
                     /locus_tag="VLF19_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HSE93804.1"
                     /translation="MRIEIICTGDEVLTGKIVNTNFSYISQKLEDVGLSVQCGTTVGD
                     DRQSLLRAFLLAGERADAVIVNGGLGPTVDDLSQEIAAQAAAVELVLNEEWLVRMEDF
                     FRRRSRVMPPNNRKQAMLPTTAEILDNPIGTACGFALDIGKARFFFTPGVPRELRRML
                     EEQVIPRLLARSGLQTAIHLKRFHSYGLGESHVDALLAGVEELVPDGSVKLGFRAHYP
                     QLETKLTARGRDMDDIRKKLVPVEAEVRKRLGNFILAEDDQTLEGVILAALASAQDSL
                     AVVETFTGGQIAARLAHLPGAEKVFRRGLVARELGELYAATGVADAATAGALTRETAE
                     TLARAARRQAGATHALAVLIDLDQGPDRIEFGGAICLAVATEGDVASRRSRIVGGREW
                     VRLGAVEMGLDCLRRYLQKLPVYERIDFEKV"
BASE COUNT          607 a         1507 c         1567 g          613 t
ORIGIN      
        1 ctctggtgct atgtccatct tcgggtgtgg tggggctccg tcggcggggg acaggtcggc
       61 ggacccgcgc ccccgacccc accccggcct acggtcggcg aacttgtgac tcgggagacc
      121 agagaggcac gccacggctt cgccggggcg tgccgagccc attactagag tcgagtctcg
      181 tcgaccttca ggcccagcca gtagcgtccg accagctcgt agtgggacag ccgcgactgg
      241 aggtgctggc tgatcacgtg gatgtcctgg aagtggcgct ggatcggatg gccctcgtag
      301 gccgcggtgg cgccggcggc gtgatagacg gcgtccacga cctgcaccgc cagccggatc
      361 gcgtgcgtgg tggccaatct caggttcgcc cgctggtcga gcgtgagcgc gcccgtcgtc
      421 gtggcctcgt gccagatctc gcggacggtc tcagccagga aggcccggcc ggatcggaga
      481 cgggcctcgg cgtgcccgac ctcgacctgg atcatcgact gatcccgcag cagcgcctgc
      541 atggcgcgcg gcgtcttggc gccggccagc tcgtagaagg cggccaggca cgtgcgggcc
      601 gtgcccagcg ccaccgaggc atcgcccgag gcgaacacca gcgtgcgggg tatccggtag
      661 agcggccccg tctccagcag cggcgccgtg gcggagagca ccgtgcgctc cgcgggcacg
      721 aagacgtcgt gcacggcgaa gtgatgcgtg cccgtgccac gcatgccccg caccttccac
      781 gtgtcgagca gctcggcctc ggcgcgcggc acgaacaggt aacgcgcttc cggctccggg
      841 ccgtccatcc ggatccggcc gttctcgatg acctgcgcgt gcgccgccac ccatgaggcg
      901 tgccggcagc cggtgctgaa gccctggcgg cccgtgaccc ggtaaccgcc cggcaccacg
      961 atcgcctggg ccgtcggcgc cggcgtgttg gagacgacgc tgcgcggggt gtcgatccag
     1021 atcgcgcgcg ccacgtcgcg cggcatccgt gccgcgtagg tcgcgaagat cgcgcactgg
     1081 ttgacgaccc agccggtgct ggcgtcggcc ttgccgatct cctcgatcac ctggacgtac
     1141 gtcggcaggt cgagctcggg gcaccccagc gcccggggca gcgccatctg gaacaggccc
     1201 gcgtcggcga tcgcctcgaa gagcgcgcgc ggcagctcgc gctcggcgtc ggtctcgtcg
     1261 gcgcaggacc ggatctggga ggccagcttg cgggcggcat cgagcggcgt cggatgatcg
     1321 gtcggtgtca tggtctcgaa tgggctccga tcagggccgc atgccgtgcg ccgacggacc
     1381 gggccggcgc aggggggcgg cgaggttggc gaaatcgcag agcaggggac gggtctcgcg
     1441 gggatcgatg atctcctcga tccagaaggt ctcggccgag cggaagggcg agcggagctt
     1501 ggtcaggcgt gtctcgatct ccgccatcct cgccttcggg tcggctgccg catcgatctc
     1561 ggcgcggtag gccgcctcga tgccgccttc gagcggcagg gagccccacc gccccgacgg
     1621 ccacgcgtag cgcgtggcga agcgcccgcc gttgcggtgc gcggccccgg cgacgccgaa
     1681 acagttgcgg acgatcacgg cgcaccacgg gacggtcgtc tgccacatcg ccgccatcgc
     1741 gcgcacgccc tgcttgatcg tgcccgtccg ctcggcgtcg aggccgatga ggaagccggg
     1801 acagtccacg aggtagacca ccggcaggtg gaaggtctgg gcgacgtcga cgaagcgctc
     1861 gaccttctgg caggtgtcgg ccccccaggc gccaccgtag aagaacgggt cgctggccat
     1921 cagcgccacc ggccagccgt ccagccgggc gaagccggtg acgatggagc gcccgtagag
     1981 cggggccatc tcgaagaacg agccgcagtc gacgagcgcc tcgacgatca cgcgcatctt
     2041 gtagggcttg cggacgtcgc gcgggatcgc gtcgacgagc cacggctccc ggcgctcggg
     2101 gtcgtcggta cgcgggcccc ggggtggcag ctcgtcgatc gacgccggca ggtaggagag
     2161 gaagcgtcgg gcccgggcga aggcctcgtc ctcggtggcc acggcgtcgt ccacggcgcc
     2221 ggccttgagc tggatctccc agccccccag ctcgttcttc gagagcttct gcccgagccg
     2281 cgcgaccacg ggcggcccgg ccacgaagag ggcggacagc tccctgacca tgaccgagta
     2341 gtgggcggcg gcgagatgcg cggcgccgag cccggccacc gagccgagtc cgagggcgac
     2401 gcgcgggacg gtcgcgatgt tgtcgacgac catctcccac ccgctgacgc ccggcacgtt
     2461 ggcgcggccg gtggtctcga tggtcttgac ggacccgccg ccgccggagc cctccacgag
     2521 gcggatcagc ggcagccgca gctcgttggc catccgctcg cacagcaggt gcttgtcctt
     2581 cagggtcgca tcggcggagc cgccgcgcac cgtgaagtcg tcgccgccga ccaccacggg
     2641 gcgcccctcg acccgcgccc gcccgaagac gaagttcgag ggcgtgaagc tctcgaggtc
     2701 attgtgctcg tcgtaggtcg ccttgccggc gatcttgccg atctcgtgga agctgccggg
     2761 atccacgagc gcgtcgatcc gctcccggat cgtcagccgc ccgccgtcgt gctgccgccg
     2821 cacgcgctcg gggccgccca gctgctcggc gagcgcctcg cggcgcttga gctcgtcgag
     2881 ctccggttcc caggtcatgg tctggcgctc ctccgtggcg tgctcgtgat cctaactcgt
     2941 ggcgatcgac cggtccagac gatcccgcgc ctctccggat gcggctgcgc gacgggacct
     3001 ctcgcgcgtt atgatcctgg cctatgcgca tcgagatcat ctgcacgggc gacgaggtcc
     3061 tgaccggaaa gatcgtcaac accaacttca gctacatcag ccagaagctc gaggacgtcg
     3121 ggctttccgt ccagtgcggg accacggtcg gcgacgaccg gcagagcctg ctgcgcgcct
     3181 tcctgctggc cggagagcgt gccgacgccg tgatcgtgaa cggagggctg gggccgaccg
     3241 tggacgacct gtcccaggag atcgccgccc aggcggcagc ggtcgagctg gtcctcaacg
     3301 aggagtggct ggtccggatg gaggacttct tccgccgccg gagccgcgtg atgccgccga
     3361 acaaccgcaa gcaggccatg ctgcccacca ctgccgagat cctcgacaat ccgatcggca
     3421 ccgcctgcgg cttcgcgctg gacatcggca aggcgcgctt cttcttcacc cccggggtcc
     3481 cccgcgagct ccggcgcatg ctcgaggagc aggtgatccc gcgcctcctc gcgcgcagcg
     3541 gcctgcagac ggcgatccac ctgaagcggt ttcattccta cgggctgggc gagtcgcacg
     3601 tcgatgccct gctcgcgggc gtggaggagc tggtgccgga cggcagcgtg aagctcggct
     3661 tccgggccca ctatccccag ctcgagacca agctcaccgc gcgcggccgg gacatggacg
     3721 acatccggaa gaagctcgtg ccggtggagg ccgaggtgcg caagcggctc ggcaacttca
     3781 tcctggccga ggacgatcag accctggagg gtgtgatcct ggccgcgctc gcgagtgccc
     3841 aggactcgct ggcggtcgtc gagaccttca ccggcggaca gatcgccgcg cggctggccc
     3901 acctgccggg ggcggagaag gtgttccgcc gcgggctcgt cgcgcgggag ctgggcgagc
     3961 tgtacgcggc caccggcgtc gcggacgcgg cgacggcggg cgcgctcacg cgcgagacgg
     4021 ccgagaccct ggcccgcgcg gcgcgccgcc aggccggcgc cacgcacgcg ctggcggtgc
     4081 tgatcgacct ggaccagggc cccgaccgga tcgagttcgg cggcgccatc tgcctggccg
     4141 tcgcgaccga gggagacgtc gcctcgcggc gcagccgcat cgtcggcggc cgggagtggg
     4201 tgcggctggg cgcggtcgag atggggctcg actgcctgcg ccggtacctc cagaagctgc
     4261 cggtctacga gaggatcgac ttcgagaagg tgtg
//
LOCUS       DATIAZ010000313         8150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1274449, whole genome shotgun sequence.
ACCESSION   DATIAZ010000313 DATIAZ010000000
VERSION     DATIAZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274449"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VLF19_10895"
     CDS             complement(<1..865)
                     /locus_tag="VLF19_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSE93805.1"
                     /translation="MASLTFHGAAGTVTGAKFLLEGGRGRVLLECGLFQGPRELRERN
                     WAAGPAPPGALQAVVLSHAHIDHSGYLPCLTRDGFAGPVYCSQGTADLLRIVLPDAAR
                     LQEEEADFRNRTGATRHHPALPLFTAADADRALRLLHAVDFGRGVEAASGFTVRLAPS
                     GHILGASIVTIEIEGRRLVYSGDLGRYGVPIMRDPEPITEADTLLVESTYGNRSHPAD
                     DGTAVIAEAVQRAASRGGAVLVPAFAVGRTQEVLYLLREMEEQGRVPRLPVYLDSPMG
                     AAATGVYARHPE"
     gene            complement(865..2094)
                     /gene="thiI"
                     /locus_tag="VLF19_10900"
     CDS             complement(865..2094)
                     /gene="thiI"
                     /locus_tag="VLF19_10900"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HSE93806.1"
                     /translation="MRAAVIVHYHEISLKRGNRPLFLRHLTRNLARATSDLGHVRVRQ
                     LPGRILLELGGHERPDAVRDRAARVCGVASAALALRVPSTVEAMKRAVAALTESRRFA
                     SFRISARRAFKTYPMTSVELNRELGAFVLERSGAFAPRVDLTAPELTVHVEVLPAETF
                     VYLDRVAGPGGLPVGASGTVAALLSGGIDSPVAAWRMMKRGCRVVFVHFHSVPYLPAT
                     SQAKARALVERLTEWQYRSELLLVPLGEIQREVTLSVPPPTRVVVYRRMMVRIAEALA
                     RRVGALALTTGESLGQVASQTLTNIARADEVATLPILRPLIGMDKLEITAEAQRLGTF
                     EISVEPDADCCTLFVPPHPSTRVSAGEIRAAEARLDVARLTALGVDGAVLERFEFPPA
                     PAAASRAPGATPPAASA"
     gene            complement(2109..2912)
                     /locus_tag="VLF19_10905"
     CDS             complement(2109..2912)
                     /locus_tag="VLF19_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93807.1"
                     /translation="MSDAIAGLNALPLPVREQVYTRLIPDELLVRYGIDRRGLRGEGG
                     ERLVRVASGEERSWVRVELRAREHDRDPVLLVDVGMSAFGAPELSLVQINDPDGPRYG
                     IDRDTDGHETLLGTAGRNLAEEERALGDGLAPGQVRPGLRMLRRVLETMDAFCNLLGS
                     DFYLVEPLFYHSALLYERHGCDYFMGRDRMEAIHAGFQSGGALHARLDGSTPFRRPGF
                     DSTIRGRSWAIHDGVLGQPFCDGASGVNVKMYRVPGRAAGVCTFPNGTY"
     gene            complement(2909..3490)
                     /locus_tag="VLF19_10910"
     CDS             complement(2909..3490)
                     /locus_tag="VLF19_10910"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HSE93808.1"
                     /translation="MIQAGGKSTRMGGDPKALMDLGGRRIIERVVDALDGVVDDLLVV
                     TNTPEVYAFLGLPMVGDAFPGGGSLGGIYSGLRAAPGEAAFTVACDMPFLKTEIVRLV
                     VARAAEGDVVIPRIGGQYETMHAAYRKSCLPHMEERLREGRLKIVGFFDRVRVVEIGE
                     VEVARHGDPTLVFMNVNTPEELARARAVAAGTR"
     gene            complement(3603..4214)
                     /locus_tag="VLF19_10915"
     CDS             complement(3603..4214)
                     /locus_tag="VLF19_10915"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSE93809.1"
                     /translation="MGTEVVAVDSEAPEPGVLSRAAGVLEAGGLVAFPTETFYGLGAS
                     ALDADAVRRVFALKGRPESRPLLVLVDGVPAAERIAEVPPHARALMDRYWPGALTLVL
                     RARPIVPDEVTAATGTVGVRVPAHAVARGLAHALGGPVTAPSANPTGAKAPTTAAEVR
                     RYFDSRLPLVLDGGPTTGGLPSTVLDVTVDPPRLLRAGAVRVK"
     gene            complement(4223..4681)
                     /gene="purE"
                     /locus_tag="VLF19_10920"
     CDS             complement(4223..4681)
                     /gene="purE"
                     /locus_tag="VLF19_10920"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSE93810.1"
                     /translation="MLEAVKVLDALEIGSEVVVASAHRTPQRTHDYGATAHVRGLGVL
                     IAAAGGAAALPGVLAAVSPLPVIGVPIEATPLGGLDALLSIVQMPKGVPVATVAVGTW
                     GAANAAILAAQILATGDLALRDRLATYRQRLADEVDERARRVADRMKARE"
     gene            complement(4797..6077)
                     /gene="purD"
                     /locus_tag="VLF19_10925"
     CDS             complement(4797..6077)
                     /gene="purD"
                     /locus_tag="VLF19_10925"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSE93811.1"
                     /translation="MKVLLVGSGGREHALAWKIAQSPLVERLYAAPGNPGIGRVATCI
                     PIPVDAHDDLLRLAVSEGIDFAIVGPEAPLVGGLADRFAESGILALGPQLKAARLEGS
                     KAFAKELMSRERIPTARFETLRDPDAARAYYQTYYQDRPVVVKADGLAAGKGAVICPT
                     LEVAVETTARMMGGMFGDAGRIVVAEEFLQGEEVSFFALSNGVDVVSLGAAQDHKTIR
                     DNDEGPNTGGMGAYSPVPAFERTLEQHVMDTIVKPTISALNNAGMPYRGVLFVGLMLT
                     KDDGPKVLEFNCRFGDPECEVLMARLNEDIVPPLHAAARGDKLPERLAWKPEAAVCVV
                     LASGGYPGPYRTGVPIEGVAAAEEIDGVRVFHAGTAESDGRLVTAGGRVLSVTATAPS
                     IGAAIDRAYAAVDRIRFDGMHSRRDIGRRAIVGS"
     gene            complement(6074..6979)
                     /locus_tag="VLF19_10930"
     CDS             complement(6074..6979)
                     /locus_tag="VLF19_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93812.1"
                     /translation="MSPRSPERVYGLRELTRVLSLTPRRVAQLRRLELLRRDSGYTFR
                     ELLALKAASALLEAGASVRQIKDALQALRRQDPDLRDPLSEVRLVMDGGRLLAESDRV
                     RFDPRTGQTVLALDAGGLEKDVQATLATGLVRPLHPPAAQAETWFERASEWDADPARW
                     EQALDAYRRVVALDPTYAAAWNNQGLLLHRLGRYEEARRAYDRALEQDPQCAEAAYNL
                     GSLDEDCGDVEGAVAHYRRALGLSPDYADAHFNIAAALARAGRGAEAVRHWQRYLELD
                     SGSPWARIARAHLELVEPPGGGTAE"
     gene            complement(6983..>8150)
                     /gene="purH"
                     /locus_tag="VLF19_10935"
     CDS             complement(6983..>8150)
                     /gene="purH"
                     /locus_tag="VLF19_10935"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSE93813.1"
                     /translation="VIDELRASGGALGAATRARLAAEAFARTSGYDAAIADYLSSEGA
                     STVASEASPQDEVARAKPALEPVDFPARLRLDAERVMRLRYGENPHQQAAFYRLGEEG
                     RVGLGAMRQLFGPELGYNNLLDFSAALGLLLEFDVPAAVVVKHTNPCGVAVGPGVAAA
                     MAKAKASDPVSIYGGIVGVNRTLDVGVVAELAGIFVEILFAPAFAPEALEELARTKKK
                     CRVLEVPCDRAAVGGRQVEYRSVLGGLLAQTADRDDLDERTLRTVTRRAPTADEQASL
                     RFAWRVAKHVKSNAIVLAGRDQVIGIGAGQMNRLDSARLAVARAREVGLKTEGAVCAS
                     DAFFPFRDGLDEIARAGATAVIQPGGSLRDDEVVSAADAHAMAMVFTGIRHFRH"
BASE COUNT         1180 a         3002 c         2884 g         1084 t
ORIGIN      
        1 cttccggatg gcgggcgtag acgcccgtcg cggccgcgcc catcgggctg tcgaggtata
       61 cgggtagccg gggtacgcgg ccctgctcct ccatctcccg cagcaggtac agcacttcct
      121 gcgtgcgccc gaccgcgaag gccggcacga gcaccgctcc accgcgcgag gccgcgcgct
      181 gcacggcctc ggcgatcacg gccgtgccgt cgtccgcggg atgcgatcgg ttgccgtagg
      241 tcgactcgac gagcagcgtg tccgcctctg tgatcggctc cggatcgcgc atgatcggca
      301 cgccgtagcg gccgaggtcg cccgagtaga ccaggcggcg tccctcgatc tcgatggtga
      361 cgatcgacgc gccgaggatg tgccccgacg gcgcgagccg cacggtgaag cccgacgccg
      421 cctcgacgcc gcggccaaaa tccacggcgt gtagcaggcg caaggcgcgg tcggcgtcgg
      481 ccgcggtgaa caggggcagc gccggatgat ggcgcgtggc gcccgtccgg ttgcggaagt
      541 cggcttcctc ttcctgcagc ctcgccgcgt cgggcagcac gatgcgcagc aggtcggccg
      601 tgccttgcga acagtacacc ggccccgcaa agccgtcgcg cgtcaggcag ggcaggtaac
      661 ccgagtggtc gatgtgcgcg tgcgagagca ccacggcctg cagcgcgccc gggggagcgg
      721 ggcccgccgc ccagtttcgc tcgcgaagct cgcgcgggcc ctggaacagc ccgcactcga
      781 gcagcacgcg gccgcggccc ccctcgagga ggaacttcgc gccggtgacg gtgccggcgg
      841 caccgtggaa ggtgagcgag gccactacgc gctcgcggcg ggcggcgtcg cgcccggcgc
      901 ccgcgaggcc gcagccgggg ccggcgggaa ctcgaagcgc tcgagcacgg cgccgtccac
      961 gcccagggcc gtgagccgcg cgacgtccag gcgggcttcc gccgcccgga tctccccggc
     1021 cgatacccgc gtcgacggat gcggtggcac gaagagcgtg cagcagtcgg catctggctc
     1081 gacggagatc tcgaacgtgc cgaggcgctg cgcctccgcg gtgatctcga gcttgtccat
     1141 gccgatcagc ggccggagga tcggcagcgt cgcgacctcg tcggcgcgcg cgatgttggt
     1201 cagggtctgg gaggccacct ggcccaggct ctctcccgtc gtcaaggcca gcgcgccgac
     1261 gcgccgcgcg agcgcctcgg cgatgcgcac catcatccgg cggtagacga cgacccgcgt
     1321 gggcggcggc accgagaggg tgacctcccg ctggatctcg ccgagcggca cgagcagcag
     1381 ctcggagcga tactgccact cggtcagccg ctcgacgagc gcgcgcgcct tcgcctgcga
     1441 ggtggcgggc aggtacggca cgctgtggaa gtgcacgaag accactcggc agccccgctt
     1501 catcatgcgc caggccgcga ccggagagtc gatcccgccg gacagcagcg cggcgacggt
     1561 gccgctggcg cccacgggca gccccccggg gccggccacc cggtcgaggt agacgaaggt
     1621 ctcagcgggc aggacctcca cgtgcacggt cagctcgggc gccgtcaggt ccacgcgcgg
     1681 cgcgaacgct cccgagcgct cgagcacgaa ggcgccgagc tcacggttca gctccacgga
     1741 cgtcatcggg tacgtcttga acgcccgtcg cgccgagatg cggaacgagg cgaagcggcg
     1801 gctctcggtg agggccgcga ccgcccgctt catggcctcc accgtggacg gaacccgcag
     1861 ggcgagggcc gcgctggcta cgccgcacac gcgcgccgca cggtcccgca cggcatccgg
     1921 ccgttcgtga ccgccgagct cgagcaggat gcggccgggc agctggcgca cacgcacgtg
     1981 gccgaggtcc gaggtggcgc gcgcgaggtt gcgggtgaga tgccggagga acagcggacg
     2041 gttgccccgc ttgaggctga tctcgtggta gtggacgatg accgcggcgc gcatgacact
     2101 cccgccgctc agtatgtgcc gttcgggaac gtgcagacgc ctgcggcgcg cccgggcacc
     2161 cggtacatct tgacgttcac gcccgaggct ccgtcgcaga acggctggcc gagcacgccg
     2221 tcgtggatcg cccagctccg gccgcgaatg gtcgagtcga agccgggacg gcggaacggc
     2281 gtggagccgt cgagccgggc gtgaagcgcg ccgccggact ggaagcccgc gtggatcgcc
     2341 tccatgcggt cgcgtcccat gaagtagtcg cagccgtgac gctcgtagag cagcgccgag
     2401 tggtagaaga gcggctccac cagatagaag tcactgccga ggagattgca gaaggcgtcc
     2461 atcgtctcca gcactcgccg cagcatgcgc aggccgggtc gtacctggcc cggcgccagt
     2521 ccgtccccca gggctcgctc ctcctcggcg aggttgcgac cggccgtgcc cagcagcgtc
     2581 tcgtgcccgt cggtgtcgcg atcgatcccg tagcgcgggc cgtcggggtc gttgatctgc
     2641 acgagcgaca gctcgggcgc cccgaacgcc gacatgccga cgtccacgag caacaccggg
     2701 tcgcggtcgt gctcgcgggc ccgcagctcc acgcgcaccc acgagcgctc ctcgccggac
     2761 gcgacccgca cgaggcgctc gcccccctcg ccacggaggc cccggcggtc gatgccgtag
     2821 cggacgagca gctcgtcggg aatcagccgc gtgtagacct gctcgcgcac cggcagcggc
     2881 agggcgttca gtcccgcgat ggcgtcgctc accgcgttcc cgccgccacc gcccgcgcgc
     2941 gggcgagctc ctcgggcgtg ttgacgttca tgaagaccag cgtcgggtcg ccgtggcgcg
     3001 ccacctcgac ctcgccgatc tccaccaccc gcacccggtc gaagaacccg acgatcttga
     3061 gccggccctc acgcaggcgt tcctccatgt gcggcaggca gctcttgcgg tacgccgcgt
     3121 gcatcgtctc gtactgtccg ccaatgcgcg gaatcacgac gtcgccctcg gcggcgcgcg
     3181 ccaccacgag acgcacgatc tcggtcttca agaacggcat gtcgcaagcc acggtgaagg
     3241 ccgcctcccc gggggcggcg cggagcccgg agtagattcc gccgagggag ccgccgcccg
     3301 ggaacgcgtc gccgaccatg ggcaggccga ggaaggcgta gacctccggt gtgttggtga
     3361 cgaccaggag gtcgtccacc acgccgtcga gcgcatcgac gacccgctcg atgatccggc
     3421 ggccgccgag atccatcagc gccttcgggt cgccgcccat ccgggtgctc ttgccgcctg
     3481 cctggatcac gccggtaatt ttcattcgga ggaggcctcg acggcctcct ccgaggcctc
     3541 ccccaggagg gatcgcgcgg gccacgccag cgctcgaacc gtggtgtcgc gacggtcggc
     3601 gatcacttca cccgcacagc gccggcacgt aggaggcgcg gcggatcgac ggtcacgtcg
     3661 agcaccgttg aaggcagtcc gccggtcgtg gggccgccgt cgaggaccag ggggagacgg
     3721 ctgtcgaagt aacggcggac ctcggcggcc gtggtcggtg ccttggcgcc ggtcgggttg
     3781 gcgctgggcg ccgtcaccgg gcctcccagt gcgtgggcca gaccacgggc cactgcgtgg
     3841 gcggggacgc ggacgcccac ggtgccggtg gcggccgtga cctcgtccgg aacgatgggg
     3901 cgagcgcgga gcaccagcgt cagcgcgccc ggccagtagc ggtccatcag cgcgcgcgcg
     3961 tgcggcggca cctcggcgat gcgctcggcc gcgggcacgc cgtcgaccag gacgagcagc
     4021 ggccgcgact cgggccgccc cttgagagcg aacacgcggc gcacggcgtc agcgtcgagc
     4081 gccgaggcgc cgaggccgta gaaggtctcc gtcgggaacg ccaccaggcc gcccgcctcg
     4141 agcacccccg cggcgcgcga gagcacgccc ggctccggag cttcggaatc gacggcgacg
     4201 acctcggtgc ccatgcgctg cgctactccc tggccttcat ccggtccgcc acccggcggg
     4261 cccgctcgtc gacctcgtcc gccaggcgct ggcggtacgt ggcgaggcgg tcgcgcaggg
     4321 cgagatcacc ggtggcgagg atctgcgccg cgaggatcgc cgcgttggcg gcgccccacg
     4381 tgccgacggc gacggtggcg acgggaacgc ccttgggcat ctggacgatg gacaggaggg
     4441 catcgagacc gccgagcggc gtggcctcga tgggcacgcc gatcaccggc agcggcgaca
     4501 ccgcggccag cacgccgggc agcgcggcgg cgccgccggc cgcggcgatg agcacgccga
     4561 ggccccgcac gtgcgccgtc gcgccgtagt cgtgcgtgcg ctgcggtgtg cggtgagccg
     4621 aagcgacgac gacctcgctg ccgatctcga gcgcgtccag caccttcacc gcctcgagca
     4681 tgacctcgag gtccgagtcg ctgccgagga cgatcccgac acgtacttgc ctggaaggct
     4741 ccgccctcgt tccggcccct ccccccttcg gcgcgcgggc ggagcccgcg cccgaactac
     4801 gagccgacga tcgcccggcg cccaatgtct ctcctggagt gcatcccatc gaaacggata
     4861 cggtcaacgg cagcataggc tcgatcgatc gcggcgccga tcgatggcgc ggtcgcggtg
     4921 acgctgagga cacggccacc ggcggtcacg agccggccgt cgctttccgc ggtgcccgcg
     4981 tggaacacgc gcacaccgtc gatctcttcg gcggcggcga cgccctcgat gggcaccccc
     5041 gtgcggtacg ggccaggata gccacccgac gcgagcacga cgcacaccgc cgcttcaggc
     5101 ttccaggcca gacgctcggg cagcttgtca ccacgggccg ccgcatgcag cggtgggacg
     5161 atgtcctcgt tcagccgcgc catcaggacc tcgcactctg gatcaccgaa gcggcagttg
     5221 aactcgagga ccttcggccc gtcgtccttc gtcagcatga gtcccacgaa cagcacgccg
     5281 cgatagggca tgcccgcgtt gttgagcgcg gagatcgtcg gcttgacgat cgtatccatc
     5341 acgtgctgct cgagagtgcg ctcgaaggcc gggaccgggg agtacgcccc catcccgccg
     5401 gtgttcggcc cctcatcgtt gtcccggatc gtcttgtggt cctgggccgc accgagtgac
     5461 acgacatcca cgccgttcga gagagcgaag aacgaaacct cttcgccctg gaggaattcc
     5521 tccgccacga cgatccggcc ggcatcgccg aacatgccgc ccatcatccg cgccgtggtc
     5581 tcgaccgcca cctcgagagt cggacagatc accgccccct ttccggcggc gagaccgtca
     5641 gccttcacga cgaccggcct atcttggtag tacgtctggt agtacgccct ggcggcatcg
     5701 ggatcccgaa gcgtctcgaa gcgcgcggtc gggatccgct cccgactcat cagttccttg
     5761 gcgaaggcct tcgagccctc cagcctggcg gccttcagct gcggtccgag ggccagaatt
     5821 ccgctctcgg cgaaacgatc ggcgagccca ccgaccagcg gggcctcggg cccgacgatc
     5881 gcgaagtcga tcccctcgct gacagcgagc ctcagcaggt cgtcgtgggc gtcgaccggg
     5941 atcgggatgc acgttgccac cctcccgatt cccggattgc ccggcgcggc gtacagtcgc
     6001 tcgacgagcg ggctctgggc gatcttccac gccagcgcgt gctcgcgacc gccgctcccg
     6061 accagcagca ccttcactcg gccgtccccc cgccgggcgg ctccaccagc tccaggtgcg
     6121 cccgcgcgat gcgggcccac gggctgccgg agtcgagctc caggtagcgc tgccagtgcc
     6181 tcaccgcctc ggcgccccgg ccggcgcgcg ccagcgcggc cgcgatgttg aagtgcgcgt
     6241 ccgcgtaatc gggggacagc cccagcgcgc ggcgatagtg ggcgaccgcg ccctctacgt
     6301 ccccgcagtc ctcgtcgagc gagccgaggt tgtatgcggc ctccgcgcac tgcgggtcct
     6361 gctcgagggc acggtcgtag gcccggcggg cctcctcgta acgcccgagg cggtgcagca
     6421 gcagcccctg attgttccag gccgcagcgt acgtcgggtc gagcgcgacc acgcgccgat
     6481 aagcatcgag cgcctgctcc cagcgcgccg gatccgcgtc ccactcgctc gcccgctcga
     6541 accacgtctc ggcttgcgcc gccggcgggt ggagtggccg gacgaggccg gtcgcgagcg
     6601 tggcctgcac gtccttctcg aggccgccgg cgtcgagtgc cagcaccgtc tggccggtgc
     6661 gcgggtcgaa gcgcacgcga tcggactccg cgagcaggcg gccgccgtcc atcacgaggc
     6721 gcacttccga cagcggatcc ctgaggtcgg gatcctggcg ccggagcgcc tggagcgcgt
     6781 ccttgatctg gcgcacggac gctcccgcct ccagcagcgc actcgcggcc ttgagggcga
     6841 gcagctcgcg gaaggtgtag cccgagtcgc gccgcagcag ctcaagccgg cggagctgcg
     6901 cgacgcgcct gggcgtgagg ctgagtacgc gggtgagctc gcgcaggccg tagacgcgct
     6961 cggggctccg ggggctcatc cctcagtgcc ggaagtgtcg gataccggtg aacaccatcg
     7021 ccatggcgtg ggcgtcggcg gcggagacga cctcgtcgtc gcggagcgag ccacccggct
     7081 ggatcacggc ggtggcgcca gcccgggcaa tctcgtcgag cccgtcacgg aacggaaaga
     7141 aggcgtcgga cgcgcagacg gcgccttccg tcttgagacc gacctcgcgc gcgcgggcca
     7201 cggccagccg cgcagagtcg agccgattca tctggccggc gccgatgccg atgacctggt
     7261 cgcgtccggc cagcacgatc gcattggact tcacgtgctt ggccacgcgc catgcgaagc
     7321 ggagcgacgc ctgctcgtcg gcagtgggcg cgcggcgagt caccgtcctg agcgtcctct
     7381 cgtcgaggtc gtcccggtcg gccgtctgcg cgagcagccc gcccagcacg ctgcggtact
     7441 ccacctgacg cccgccgacg gcggcgcggt cacagggcac ctccagcacc cggcacttct
     7501 tcttcgtgcg cgcgagctcc tcgagcgcct ccggcgcgaa tgccggggcg aagaggatct
     7561 cgacgaagat gcccgcgagc tcggcgacca cccccacgtc gagcgtgcgg ttgacgccga
     7621 cgatcccgcc gtagatcgac acggggtcgg acgccttggc cttggccatc gccgcggcga
     7681 cgcccggtcc gacggccacg ccgcagggat tggtgtgctt gacgacgacc gcggccggta
     7741 cgtcgaactc gagcagcagc ccgagcgcag ccgagaagtc gaggaggttg ttgtatccca
     7801 gctccggccc gaacagctgc cgcatcgccc ccagccctac ccgcccctcc tcccccaggc
     7861 gatagaacgc cgcctgctga tgtggattct ccccgtaccg aagtctcatc acgcgctccg
     7921 cgtccagtcg tagccgtgcg gggaaatcca caggttcgag cgcgggcttt gcccgcgcaa
     7981 cctcgtcctg gggggaggcc tcggaggcga ccgtcgaggc cccctccgag gacagatagt
     8041 cggcgatcgc ggcgtcgtag cccgacgtgc gcgcgaacgc ctcggccgcc aggcgcgccc
     8101 gcgtcgcggc gccgagcgca ccgccgctcg ctcgcagctc gtcgatcacg
//
LOCUS       DATIAZ010000314        10890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1407743, whole genome shotgun sequence.
ACCESSION   DATIAZ010000314 DATIAZ010000000
VERSION     DATIAZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407743"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..469
                     /locus_tag="VLF19_10940"
     CDS             134..469
                     /locus_tag="VLF19_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93814.1"
                     /translation="MKTLVSALVLTLATLWGLPALAADDSKVKAATNQVETGAKKIGQ
                     GKVGEGVEETAKGIGKTVVEGAKFTGRKFEEAGKAAEPKAKSTWENVKEGATSFGQSV
                     KSFFGRLFQ"
     gene            complement(480..1652)
                     /locus_tag="VLF19_10945"
     CDS             complement(480..1652)
                     /locus_tag="VLF19_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HSE93815.1"
                     /translation="MSRVQTGLDVLVRRRGAPLSGRRVGLLAHQASVDARLEHAAAVL
                     ARLPRVRLVRLLAPEHGLWGAAQDHAAIATARDPVTGLRVESLYGARRAPTPAMLRGL
                     DALVVDLQDVGSRYYTFIWTTALAMAACARADVEVVVLDRPNPLGGVLVDGNVAEPAF
                     ASFVGLYPLPVRHGLTIGELARYLNVEHALGCRLAVVPMRGWRRRMAWEDTGVPWVAP
                     SPNMPTPDTARVYPGGCLIEGTNLSEGRGTTRPFEWVGAPWLDAHRYAAALNDLGLPG
                     VRFRPARFRPTFHKWAGRLCGGVQVHVTDREAFPAFLAGLALIAVARRQAPGRFAWKR
                     PPYEFERRRLPIDILCGTGAIRRAIEGGVRLSSLPRGWRPQLRRWLRRRAPYLLYR"
     gene            1692..2597
                     /gene="murQ"
                     /locus_tag="VLF19_10950"
     CDS             1692..2597
                     /gene="murQ"
                     /locus_tag="VLF19_10950"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HSE93816.1"
                     /translation="MGLRPRYDRLATERANRASRDLHRMTPLEIARLMNREDARAVRA
                     VGRALPAISRAVDAIVTALASGGRLFFVGAGTSGRLGVLEAAECPPTFGTPPTLVQAV
                     MAGGRASVFRSREGAEDDAAASARAVHARVRRGDAVVGVSASAVTPFVRGALAAARRA
                     GATTVLVACNPHAARIGVPRGTITVALDPGPEIVTGSTRLKAGTATKLVLNTLTTASM
                     ARLGRVWGNRMVDVQPRSRKLRERAMRLVTELGGVNRRGAARALAASGDRVRLAVVVA
                     RTGWPPPRARRALREAGGSLRPFLP"
     gene            2878..3423
                     /locus_tag="VLF19_10955"
     CDS             2878..3423
                     /locus_tag="VLF19_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93817.1"
                     /translation="MLVRRCAWHRRYHGYPWLYGVASWRGEGLDFTDGLCPDCAVRAR
                     EDIGIPDPVPAVGPRRALALVAAFTFLLAPEVLDQFPAGSFHLPSLLRPAALVPKTLA
                     VRDAAADEPPPAPAADVASVLALVTAAASAIDGPVAGPPPAGPPMRDPPDRVLPLPMR
                     SQLRFYDDPTSPLAAYDVQAP"
     gene            complement(4061..5794)
                     /locus_tag="VLF19_10960"
     CDS             complement(4061..5794)
                     /locus_tag="VLF19_10960"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HSE93818.1"
                     /translation="MTAQPLRSRNWFGRRDLDGFAHRSWLKAEGFSDLVFDGRPVIGV
                     ANSWSELTNCNAHLRQVADAVKRGVWSAGGLPLEFPTISLGEVLMKPTAMLFRNLMAM
                     DVEECIRAYPLDAVVLLSGCDKTTPAMLMGAASADVPAIMVTGGPMLRGRWRAEELGS
                     GTDLWRLWAERRAGRLSDEELCEAESCMSRSAGHCMVMGTASTMASMAEALGMTLPGN
                     AAIPAPDSRRLALAEMSGRRAVEMAQAGGPRPSEVLTTRAFDNAIRADMAIGGSTNAI
                     IHLVAIAGRAGVPLPLRRFDELSKTTPLLANLRPSGKHLMEDFFDAGGLPVVLRRLLP
                     LLHGDALTVNGRTLADNVRDAHCWNDDVIRPLATPLAAEGGTVVLTGNLCPDGAVLKQ
                     SAASPHLFSHRGRAVVFEDHDDLHRRIDDERVPVDETSVLVLKHVGPRGAPGMPEWGA
                     APIPERLLRRGVNDMVRISDARMSGTSYGTVVLHVAPESAVGGPLALVRDGDEIELNV
                     PARTLTLRVADEELARRRAAWRSRPPRFERGYGRLFLDHVLQANEGCDFDFLRGRTAV
                     RLEDTLGPSHA"
     gene            5846..6643
                     /locus_tag="VLF19_10965"
     CDS             5846..6643
                     /locus_tag="VLF19_10965"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93819.1"
                     /translation="MRLAGKVAVVTGGGSGIGRGIVLAMAREGADIAIPDIQVLNAEK
                     VAEEVRGLGRRVVAMKTDVTSSADVKAMVERTREALGKIDILVNNAGMAAPPGMPFTS
                     NTEEDWDRTFAVNTKSVFLTCKAVAPYFVERKAGRIINIASIAGPLSALTMPPYSVAK
                     QGVITFTRIVAKELAPHRITANAICPGVLYTAFWEKLAAHIAQTNPAFAGMTPRQVFE
                     KRVADIVPMKCEQTPEDIGWAAVFLASDEARYITGQALMVDGGCVMW"
     gene            6748..7926
                     /locus_tag="VLF19_10970"
     CDS             6748..7926
                     /locus_tag="VLF19_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE93820.1"
                     /translation="MDFDFTPEQEGFRKEVRQWLERTLPDDLRGRAFAASRADVDEVR
                     RLRAWQKTMYEAGYVGIDWPREFGGRGASIVEVIILYQEMARAESPQIVNRGGVSMLG
                     PTLMTHGTPAQQKRFLAKILSAEELWCQGFSEPNAGSDLANLQTRAVRDGDHFVVNGQ
                     KVWTSMGHVADWCFVLVRTDPDAPKHKGLSFLLMDMRSPGVTVRPLRQITGEAEFNEM
                     FLDNVRIPAENLVGRPNEGWGVALTTLAYERDVLTFIRHISLRNALHRLVRLVRERDR
                     AADLVVRQKVAALWIGEQALQLNAYRSLTRMLKGGQPGPEGSTSKLFWSHLDQELAQL
                     GTEVIGPYSQIAPGSPWAPDEGQWEFYELLARGSGIRAGTTEILHNILGERVLGLPKD
                     "
     gene            7988..8770
                     /locus_tag="VLF19_10975"
     CDS             7988..8770
                     /locus_tag="VLF19_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSE93821.1"
                     /translation="MTDLIYEVKDRVATITLNRPDKLNAFTGPMIDAWATSLAEAQRD
                     DGVNVVVVTGAGRAFCAGGDVGRMGESRPSPLDHKNQLWENIHRVPKTLEAMDKPVIA
                     MVNGLAVGAGMGMCVMCDVRVAAESARFSTGYVRVGLVPGDGDTYFLPRLVGAAKALE
                     LLWTADFIEAPEAHRLGVVQRVVPDAQLKDATYALARQIADGPQVPIRMIKRLVYQSL
                     KLDLRTHLDLVSSHMSIVRQTADHAEGVAAFKEKRPPKFRGR"
     gene            8895..9413
                     /locus_tag="VLF19_10980"
     CDS             8895..9413
                     /locus_tag="VLF19_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93822.1"
                     /translation="MDLYEAKDRVAVALVESIFRRARYCVQPYRPEPSEVRFVREDFS
                     PSFTAVLPGEDGAERAFLLDVAYRPFVEQFMALENQRRTSSIFVLARRHWPSLRSVLV
                     TDHPEPGRSRFQTVVVGDLSSDPMLTTVDLTDADALGIFRHNVEDHEELLERIFTMLL
                     TDRHRREASRVG"
     gene            complement(9426..10124)
                     /locus_tag="VLF19_10985"
     CDS             complement(9426..10124)
                     /locus_tag="VLF19_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HSE93823.1"
                     /translation="MTVPAAHPERHFTGSRTVRDLVLGMADGLTVPFALAAGLTGAID
                     TAWIIVVAGLAEIAAGAIAMGLGGYLAAKSDVEHYESEHAREHREVREKPEAEAAEVL
                     EMLETYGVKPEEARPIVDSLRRNPQRWVDFMMRFELGLERPDPRRALASALTIGGAYA
                     VGGLIPLWPYMAFAGVARALAVSVGVTAVALFAFGYVKGRFTGAPPVRSAVQTALIGG
                     VAAGVAFLIARALA"
     gene            complement(10130..10654)
                     /locus_tag="VLF19_10990"
     CDS             complement(10130..10654)
                     /locus_tag="VLF19_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015628998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSE93824.1"
                     /translation="MPLYSFEGKRPHVHPSAFVAPTAVLIGDVTVEENASVWYNAVLR
                     ADFGPIVIRRGANVQDGAVVHVTPVHPVEVGPGATIGHTCVVHGATLGEEALVGNGAT
                     VLDGARIGARAMVGAGALVTPDSEIPAGMLALGAPAKVRGPLAGTPAEFWVRANPAAY
                     QALAQRHRAGVAPA"
BASE COUNT         1585 a         3855 c         3936 g         1514 t
ORIGIN      
        1 gttcacgtga ctatctgagc tgatagccgc gtgaccggcg cccgccccgc gtgccctccg
       61 cggggcggtc gttttttcag cgctcgttgg taggatgaat gcaggcgttc ctttcctcgg
      121 agggaggggg gcgatgaaaa cactcgtcag cgcactggtt ctcaccctgg caacgctctg
      181 gggactgccc gcgttggctg cggacgactc caaggtcaag gccgccacga accaggtcga
      241 gaccggcgcc aagaagatcg ggcagggcaa ggttggcgaa ggcgtggagg agacggccaa
      301 gggcatcggc aagaccgtgg tcgagggcgc caagttcacc gggcgcaagt tcgaggaggc
      361 cgggaaggcc gccgagccga aggccaagag cacctgggag aacgtgaagg agggcgccac
      421 cagcttcggc cagagcgtca agagcttctt cggccggctc tttcaatgat tcagcgggct
      481 cagcggtaga gcaggtacgg tgcccgcctt cggagccagc gccgtagctg cggtctccag
      541 ccccggggca gcgaggatag ccggacgccg ccctcgatcg cacgtcggat cgcgccggtc
      601 ccgcacagga tgtcgatcgg cagccgccgg cgctcgaact cgtagggcgg gcgcttccat
      661 gcgaatcgcc cgggcgcctg cctgcgggcg acggcgatga gcgccaaacc cgccaggaag
      721 gcagggaagg cctcgcgatc cgtcacgtgc acctggacgc cgccgcagag gcgtccagcc
      781 cacttgtgga aggtcgggcg gaagcgcgcc gggcggaagc gtacgccggg caggccgaga
      841 tcgttcaacg ctgccgcgta gcggtgcgcg tccagccagg gcgcgcccac ccactcgaac
      901 gggcgggtgg tgccacgccc ttccgagagg ttggtcccct cgatcaggca cccgccggga
      961 tagacacgcg cggtgtcggg agtcggcatg ttcggcgatg gcgccaccca cggcacgccg
     1021 gtgtcctccc aggccatccg gcgccgccag ccgcgcatgg gaaccacggc gagacggcag
     1081 ccaagcgcgt gctcgacgtt caggtatcgt gccagttcgc cgatggtcag gccgtggcgc
     1141 accggcagcg ggtagaggcc cacgaacgag gcgaaggcgg gctcggcgac gttgccatcc
     1201 acgaggacgc cgccgagcgg gttggggcga tccagcacca cgacctcgac gtccgcccgg
     1261 gcgcacgcgg ccatggccag cgctgtggtc cagatgaacg tgtagtagcg ggacccgacg
     1321 tcctggaggt cgacgacgag cgcgtccagc cctcgcagca tcgctggcgt cggcgcccgg
     1381 cgcgcaccgt agaggctctc cacccggagg cccgtgacgg ggtcgcgcgc ggtggcgatc
     1441 gccgcgtggt cctgcgcggc gccccacagc ccgtgctcgg gcgccaggag ccgcacgagc
     1501 cggacgcgcg gcagccgggc cagcacggcc gcggcgtgct cgagcctggc gtccacggag
     1561 gcctggtgcg cgagcaggcc gacacgccga cccgagagcg gcgctccgcg gcggcgcacc
     1621 agcacgtcga ggcccgtctg cacgcgcgac acgccgacga tggtagcagc ccgtagtacg
     1681 atacgacgac catggggctg cgcccgcgct acgaccgcct cgcgacggag cgggccaacc
     1741 gcgcgagtcg cgatcttcac cgcatgaccc cgctcgagat cgcacgcctc atgaaccgcg
     1801 aggacgcgcg cgcggtccgc gccgtcggcc gcgcgctgcc cgccatctcg cgcgcggtgg
     1861 acgccatcgt gacggcgctg gcgagcgggg gccggctctt cttcgtcggt gccggcacga
     1921 gcggccggct cggcgtgctc gaggccgccg agtgtccacc gacgttcggg acgccgccga
     1981 ccctggtgca ggccgtgatg gcgggcgggc gcgcctcggt gttccgctcg cgcgagggcg
     2041 cggaggacga tgcggcggcg tcggcccgcg ccgtgcacgc ccgcgtgcgc cgaggcgacg
     2101 ccgtcgtcgg cgtgtcggcg agcgcggtca cgccgttcgt gcgcggcgca ctcgcggcgg
     2161 cgcggcgcgc cggcgcgacg acggtgctcg tggcctgcaa cccgcacgcg gcgcggatcg
     2221 gagtcccgcg cggcacgatc acggtcgcgc tcgaccccgg tcccgagatc gtcacgggct
     2281 cgactcgact gaaggccggg accgcgacca agctcgtgct gaacacgctg acgacggcga
     2341 gcatggcgcg cctcggacgg gtgtggggca accggatggt ggacgtgcag ccgcgctcgc
     2401 gcaagcttcg cgaacgcgcg atgcggctcg tcaccgagct gggcggcgtg aatcgccgcg
     2461 gcgcggcgcg ggcgctcgcc gcctcgggcg accgcgtgcg cctggccgtc gtcgtcgctc
     2521 gcaccggctg gccgccgccg cgcgcccgac gcgccctgcg cgaggccggc ggctcgcttc
     2581 ggcccttcct tccctgagcc gggctccggc gccgccaggg cgcgtcgagc accgcaggaa
     2641 agaggtatat ctcggggccc tggaagcacg cgagatcgag gtgcggcccg gcgatggcgg
     2701 gctgtgtggt gggtccgggg gctgtccgcg cgcccaattc cccagtccaa tgtcgtcacg
     2761 tcacagtcaa aacgcattac tgtttccgtt gagaacagcc tcgtcgcgtc ttgggtgcaa
     2821 ataactgggg aatcggttag gagcaattcg gcacacatct tgcctgccca ggtaattgtg
     2881 ctggttcgtc gctgcgcatg gcatcgcagg tatcacggct acccgtggtt atacggtgtg
     2941 gcctcctggc gtggggaagg gctcgacttc accgacggtc tctgccctga ctgcgctgtt
     3001 cgggctcgcg aggacatagg gatccccgac cccgtccctg cggtcggacc acgtcgagcg
     3061 ctggcgctgg tcgcggcctt caccttcctc ctggctcccg aggtcctcga ccagtttccg
     3121 gccgggagct tccacctgcc gtcgttgctg cggccggcgg cgctggtccc caagaccctc
     3181 gccgtccgcg acgctgcggc cgacgaaccg cctccggctc cggcggcgga cgtcgcctcc
     3241 gtcctcgccc ttgtgactgc ggccgcgagc gcgatcgacg gccccgttgc ggggccgccg
     3301 cccgccgggc caccgatgcg cgaccctcct gatcgcgttc tcccgctgcc gatgcgctct
     3361 cagttgcggt tctacgacga ccccacctcg ccgctcgctg cctacgacgt ccaggcgccc
     3421 tgattcgcgg caacccgctg cacggttagt gcgcggcaca cgcgttgctc ctcgtcgggc
     3481 gtggtcatcc gtcgttgcag ccggcaccgt caccatcacg gtcatcacgc tctcttacgg
     3541 gatcgcggcg tggaggggct ggaatgtgcg attcacggaa tggatctgcc gaagttgcgc
     3601 acggcggctc cgtcgtgagc tgccggcgcc gggctggccg ggcgcggcga gcctcaaggg
     3661 tggcgcgccg ccgatgccgg tgccgcccct ggcgattggc ctgatcgctc tctcggcggt
     3721 ggttttcgtg gcacgcccgc tcgactcgcc tccgcgaatg accactcccg acgcgccgag
     3781 tctcggtggc gagcccgcgc ccgtcgatcg ccgcgcccgc tctcccgggg cgggctccgt
     3841 ccccggtacc ggccatgaac cgggcgtggg gcaggcgtcg aacagccgcc acgcgcgcgg
     3901 ggcggggcgc cacgtggcgc tcccggggag ggtctcctct tggtcggggc ccgcgcggcg
     3961 ttccacggca gcgccgcgcc ccgcacgagc ttcggtggct ccgagggagc tggcgggcat
     4021 tcaagcgcct tagcgccgcc gggccctccc cccctctcgg tcaggcgtgc gaggggccca
     4081 gcgtgtcctc gagccgtacg gctgtgcggc cccgcaggaa atcgaagtcg cacccctcgt
     4141 tcgcttggag cacgtggtcc aggaacaggc gtccgtagcc gcgctcgaag cgtggcggcc
     4201 gggaccgcca cgctgcgcgg cggcgggcga gctcctcgtc ggccaccctc agcgtgaggg
     4261 tgcgggccgg cacgttcagc tcgatctcgt ccccgtcgcg gacgagcgcg agcggcccgc
     4321 ccacggccga ctcgggcgcc acgtggagca cgacggtgcc gtacgaggtc ccgctcatgc
     4381 gcgcatcgga gatgcgcacc atgtcattga cgccgcgcct gagcaggcgc tcggggatcg
     4441 gggccgcgcc ccactccggc atccccggag ccccgcgcgg cccgacgtgc ttgaggacga
     4501 gcacggacgt ctcgtccacg ggcacgcgct cgtcgtcgat gcgtcggtgc aggtcgtcgt
     4561 gatcctcgaa caccacggcg cggccgcggt gcgagaagag gtggggggag gccgcggact
     4621 gcttgagcac ggcgccgtcg gggcacaggt tgccggtgag gaccaccgtg ccgccctcgg
     4681 cggccagcgg cgtggccagc gggcgaatca cgtcgtcgtt ccagcagtgc gcgtcgcgga
     4741 cgttgtcggc gagcgtgcgc ccgttgacgg tgagcgcgtc gccgtggagc agcggcagga
     4801 gccgacgcag cacgacgggc aggccacccg cgtcgaagaa gtcctccatg aggtgcttgc
     4861 cggacggacg cagattggcg agtagggggg tggtcttgga cagctcgtcg aagcggcgca
     4921 gcggaagcgg gacgccggcg cgccctgcga tcgccaccag gtggatgatc gcgttggtac
     4981 tcccgccgat ggccatgtcg gctcgaatcg cgttgtcgaa ggctcgcgtg gtgagtacct
     5041 cggacgggcg cggcccgccg gcctgcgcca tctccacggc gcgccggccg gacatctccg
     5101 ccagcgcgag acgccgcgag tcgggcgcgg ggatcgccgc gttgcccggc agcgtcatgc
     5161 ccagcgcctc ggccatcgag gccatcgtcg aggcggtgcc catcaccatg cagtggccgg
     5221 ccgagcgcga catgcacgac tccgcctcgc acagctcctc gtcggagagc cgccccgcgc
     5281 ggcgctcggc ccagagccgc cagagatcgg tgcccgagcc aagctcctcc gcccgccacc
     5341 tgccgcgcag catcgggccc ccggtgacca tgatggccgg gacgtcggcg gaggccgcgc
     5401 ccatcagcat ggccggtgtc gtcttgtcgc atcccgagag gagcacgacg gcgtcgagcg
     5461 gataggcgcg gatgcattcc tcgacgtcca tggccatgag gttgcggaag agcatcgccg
     5521 tcggcttcat gaggacctcg ccgagcgaga tcgtcgggaa ctcgagcggc aagccgcccg
     5581 ccgaccagac gccacgcttg accgcgtcgg cgacctggcg caggtgcgcg ttgcagttgg
     5641 tcagctctga ccaggagttg gcgacgccga tcaccggtcg gccgtcgaag acgagatcgc
     5701 tgaagccctc ggctttgagc cacgagcgat gcgcgaaccc gtcgagatcg cggcggccga
     5761 accagttgcg actccggagc ggctgtgctg tcattgggac cctcccgggg cgctgattat
     5821 atcgacggat cgatcggagg atcggatgcg gctcgccggc aaggtcgcgg tggtcacggg
     5881 cgggggcagc gggatcgggc gcggcatcgt gctggcgatg gcgcgcgagg gcgccgacat
     5941 tgccatcccc gacatccagg tgctgaacgc cgagaaggtc gccgaggagg tcagggggct
     6001 cgggcgtcgt gtcgtcgcga tgaagaccga cgtcaccagc tccgccgacg tgaaggccat
     6061 ggtcgagcgc acccgcgagg cgctgggcaa gatcgacatc ctcgtgaaca acgccgggat
     6121 ggccgcgccg cccggcatgc cgttcacgag caacaccgag gaagactggg atcgcacgtt
     6181 cgccgtcaac accaagtcgg tcttcctcac ctgtaaggcg gtcgccccgt acttcgtcga
     6241 gcgcaaggct gggcgcatca tcaacatcgc ctcgatcgcc ggcccgctgt cggcgctgac
     6301 catgccaccc tacagcgtgg ccaagcaagg cgtcatcacg ttcacgcgca tcgtggccaa
     6361 ggagctggcg ccgcaccgca tcaccgccaa cgccatctgc cccggcgtgc tctacacggc
     6421 gttctgggag aagctcgccg cgcacatcgc ccagaccaac cccgccttcg ccggcatgac
     6481 gccgcgccag gtgttcgaga agcgcgtggc cgacatcgtg ccgatgaagt gcgagcagac
     6541 gccggaggac atcggctggg cagcggtgtt cctcgcctcc gacgaggcgc gctacatcac
     6601 cggccaggcc ttgatggtcg acggcgggtg cgtcatgtgg tagtgcgagg ggagccgccc
     6661 tcggggcggc gagccgagcg gtgagatggc cgaggatggg agggacgggg atccgggcgg
     6721 tgcaatgacc gagatcgcag ggtagccatg gacttcgact tcacgcccga gcaggaaggc
     6781 ttccgcaagg aggtccgcca gtggctggag cggaccctcc ccgacgacct ccgcggccgc
     6841 gcattcgcgg cgtcgcgcgc cgacgtggac gaggtgcgcc ggctgcgcgc ctggcagaag
     6901 accatgtacg aggccggcta cgtgggcatc gactggccgc gggagttcgg cggccgtggc
     6961 gcgtcgatcg tggaggtcat catcctctac caggagatgg cccgcgcgga gtcgccgcag
     7021 atcgtcaatc gcggcggcgt ctcgatgctg gggccgacgc tcatgacaca cggcacgccg
     7081 gcgcagcaga agcgattcct ggcgaagatc ctcagcgccg aggagctgtg gtgccagggg
     7141 ttctccgagc ccaacgcggg ctcggacctc gcgaacctcc agacgcgggc ggtgcgggac
     7201 ggcgaccact tcgtggtcaa cggccagaag gtctggacgt ccatgggcca cgtcgccgac
     7261 tggtgcttcg tgctcgtgcg cacggatccc gacgcgccca agcacaaggg tctgagcttc
     7321 ctgctcatgg acatgaggtc tcccggggtg acggtccggc ccctgcgcca gatcaccggc
     7381 gaggccgagt tcaacgagat gttcctcgac aacgtccgca tcccggcgga gaacctcgtc
     7441 gggcggccga acgagggctg gggcgtggcg ctgaccacgc tggcctacga gcgcgacgtg
     7501 ctcacgttca tccgccacat ctcgctgcgc aacgcgctgc accgtctcgt caggctcgtg
     7561 cgcgagcgcg accgcgcggc cgacctggtc gtacgccaga aggtcgccgc gctctggatc
     7621 ggcgagcagg ccctgcaact caacgcctac cgcagcctca cgcggatgct caagggcggc
     7681 cagcccggcc ccgagggctc cacgtccaag ctcttctgga gccacctcga ccaggagctg
     7741 gcgcagctgg gcaccgaggt catcgggccg tattcgcaga tcgcgccggg ctcgccgtgg
     7801 gcgcccgacg agggccagtg ggaattctac gagctgctcg cgcgaggcag cggcattcgc
     7861 gcgggcacca cggagatcct ccacaacatc ctcggcgagc gcgtgctcgg cttgcccaag
     7921 gactgacgcg cggtgcggcg tgcgccggcc cacgccgcac gccacgcgca ggcttcggag
     7981 aatagcgatg accgacttga tctacgaggt gaaggaccgt gtcgccacga tcacgctgaa
     8041 ccgccccgac aagctcaacg cgttcacggg gccgatgatc gacgcgtggg cgacgtcgct
     8101 ggccgaggcg cagcgggacg acggcgtcaa cgtggtcgtg gtcaccggcg cgggccgtgc
     8161 cttctgcgcc ggtggcgacg tcggacggat gggcgagagc cggccctcgc cgctcgacca
     8221 caagaaccag ctctgggaga acatccaccg cgtgccgaag acgctggagg cgatggacaa
     8281 gcccgtcatc gccatggtca acggcctcgc cgtcggcgcg ggcatgggca tgtgcgtgat
     8341 gtgcgacgtg cgggtcgccg ccgagtccgc gcgcttctcg accggatacg tgcgggtcgg
     8401 gctcgtgccc ggcgacggcg acacctactt cctgccgcgt ctggtcggcg ccgccaaggc
     8461 gctggagctg ctatggaccg ccgacttcat cgaggcgccc gaggctcacc ggctgggcgt
     8521 cgtccagcgc gtggtgcccg acgcacagct gaaggacgcc acttacgccc tcgcccggca
     8581 gatcgccgac ggcccgcagg tgcccatccg catgatcaag cggctcgtct accagagcct
     8641 caagctcgac ctccggaccc atctcgacct cgtctcctct cacatgtcga tcgtgcgcca
     8701 gacggccgac cacgccgaag gggtcgcggc gttcaaggag aagcgcccac caaagttccg
     8761 aggccgctga gcacggccgg actgtccact gtgcgacgag ggctgccagc ctggcacgcc
     8821 acggggggcg gagccgcgga atcccgggga aaccgcgaga acgccgtggc cccgctcttg
     8881 ctcgacaagg ctgcgtggac ctctacgagg cgaaggaccg cgtggccgtg gcgctggtgg
     8941 agtcgatctt ccgccgggcc cgttactgtg tgcagcccta ccgccccgaa ccgtccgagg
     9001 tccggttcgt gcgcgaggac ttcagtccca gcttcacggc ggtgcttccc ggcgaggacg
     9061 gagccgagcg ggccttcttg ctggacgtcg cctaccggcc cttcgtcgag cagttcatgg
     9121 cgctcgagaa ccagcgccga acgtcgtcga tcttcgtgct cgcgcgccgt cactggccga
     9181 gcctgcgctc cgtcctcgtg acggaccacc ccgagcccgg ccggtcgcgc ttccagacag
     9241 tcgtcgtcgg cgacctgagc tccgacccca tgctcaccac cgtcgatctc accgacgcgg
     9301 acgcgctggg catcttccgg cacaacgtcg aggaccacga ggagctgctc gagcgcatct
     9361 tcacgatgct gctgaccgac cgtcaccggc gcgaggcgtc gcgcgtcggc tgacccggcc
     9421 cgcggtcagg cgagcgcgcg ggcgatcagg aacgccacgc cggcggcgac ccctccgatc
     9481 agcgcggtct ggacggcgct gcggaccggc ggcgcaccgg tgaagcgacc cttcacgtaa
     9541 ccgaacgcga acagcgccac cgcggtcacg ccgaccgaga cggcgagcgc ccgggcgacg
     9601 ccggcgaacg ccatgtacgg ccagagcggg atcaggccgc ccacggcgta cgcgccgccg
     9661 atggtgagcg cgctcgccag cgcgcggcgc gggtcggggc gctcgaggcc cagctcgaag
     9721 cgcatcatga aatccaccca ccgctgcggg ttgcggcgca gcgagtcgac gatcggccgg
     9781 gcctcttccg gtttcacgcc gtaggtctcc agcatctcca gcacctcggc ggcctcggct
     9841 tccggcttct cgcgcacctc ccggtgctct cgggcatgct cgctctcgta gtgctcgacg
     9901 tcgctcttgg ccgcgaggta gccgccgagc cccatggcga tggcgcccgc cgcgatctcg
     9961 gcgagcccgg cgacgacgat gatccacgcg gtgtcgatgg cgccggtgag cccggccgcg
    10021 agcgcgaagg ggacggtcag gccgtcggcc atgcccagca cgagatcgcg gacggtgcgg
    10081 ctgccggtga agtgacgctc ggggtgcgcg gcggggacag tcacggcagt caggcgggcg
    10141 ccactccggc ccggtgccgc tgcgcgaggg cctgataggc ggcggggttc gcgcgcaccc
    10201 agaactccgc gggcgtgccg gcgagcgggc cgcggacctt ggccggcgcg cccagcgcga
    10261 gcatgcccgc ggggatctcg gagtccggcg tcaccagcgc gccggcgccc accatcgcgc
    10321 gcgcgccgat gcgcgcgccg tcgagcacgg tggcgccgtt gccgacgagc gcctcctcgc
    10381 cgagcgtggc gccgtggacg acgcacgtgt ggccgatggt cgcgccgggg ccgacctcca
    10441 cggggtgcac cggcgtgacg tgcacgacgg cgccgtcctg cacgttggcg ccgcggcgga
    10501 tcacgatggg tccgaagtcc gcgcgaagca ccgcgttgta ccagacggat gcgttctcct
    10561 cgacggtgac gtcgccgatc agcacggcgg tcggtgcgac gaaggccgac ggatgcacgt
    10621 gggggcgctt tccctcgaag ctgtagagtg gcatgcggcg agacctcgca gtatgctcgg
    10681 atcggctcct atcttacgcg aacgagcgca gacggagggg cgcatggcgt atccgaggat
    10741 cgccgccgtc gcgacggcga caccgccctg gtgctacagc caggccgacg tgctgcgcgc
    10801 ggccggatac accgatgcgc agcgacgggg gttcttcgag cacagcgaca tcgacagccg
    10861 ccatctctgg atcgctccgg accggctgcg
//
LOCUS       DATIAZ010000315         4703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_486955, whole genome shotgun sequence.
ACCESSION   DATIAZ010000315 DATIAZ010000000
VERSION     DATIAZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486955"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VLF19_10995"
     CDS             complement(<1..1305)
                     /locus_tag="VLF19_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE93825.1"
                     /translation="MDSRIRRWTGMTAVVAFGLALAPAGALAKTDIQMWIGISGQLAE
                     TVESQAREFSQSQPDYEVKVVRKGSYAETLTSAVAAYRQKNPPHIVQVFEVGTQTMLL
                     SGAVYPVYELMQQNEIKINWNDFIKPVVGYYTKDGKLYSMPYNSSTPILYYNKDAFKK
                     AGLDPEKPPKAWKDLEAAARKLLASGAAKCGFSSSWPSWIQLENLHATHDQPFATKNN
                     GFGGIEGVELLINRELGQKHIGALAAWQKEHIFSYGGRGGASEPKFINGECAMQFASS
                     ALIGGFTRSAKFDWGTAELPHWGAPYRKATSIIGGATLWVLKGKPQADYRGVAKFLEY
                     LARPEQQMWWHVNTGYLAISNTAVKNLEEGYHFVRNPKQYTAFAQLTGLPPTPPSALK
                     SGKGPAAKPERVATPNSQGLRLGNFVQIRDIIEGEMENVFAGK"
     gene            complement(1371..2999)
                     /locus_tag="VLF19_11000"
     CDS             complement(1371..2999)
                     /locus_tag="VLF19_11000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HSE93826.1"
                     /translation="MSRPFPLTDGTYDVVILGGGMAGAGVARELALRGASVALFDKGD
                     FASGTTSRSSKLVHGGLRYLELFDFGLVRESLRERETLRRLAPHLVRPLPFLVPVYRE
                     SSRGLVKVRIGLKLYDWLTPGRDREPYRVLKPVDALSLEPGIRAQDLKGSGYYFDDLL
                     VYPERLCLENVLSACRHGARAFNYADVEEIVRDPRGRVVGVKARDLLTGRVATLGARV
                     VVNATGPWVDLVRERAGVADRGPRILRRTKGIHCLLPRLSNHAVYHSTRDDRMIFVIP
                     WRGFSLVGSTDTDFDGDLDRLHATREEVTYLLDEVRKVVPDPRVTLDRVAYTYAGVRP
                     LTFEEGKRASDVSRAHRVVSEAEGTFLSITGTKLTCYRSLAEGLGDQVLEALGRHAPS
                     RSGQLTLDGADEEPGRVEARTWLDVSPEMAASGLGRETLETLVTLYGRGYRRVIELAG
                     KIPGGGERLCPENPDIVAQLHLAVQDELAVSLQDVLLRRTGIGTSRCQGVDCAEGIGQ
                     RLAQLAGWTARRLGAELDAYRAHVARSQRFRAER"
     gene            complement(2996..3787)
                     /locus_tag="VLF19_11005"
     CDS             complement(2996..3787)
                     /locus_tag="VLF19_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HSE93827.1"
                     /translation="MTPRIRLPHRGWLFDLDGTIYRGDVLIPGAAETVATLRAEGRRV
                     AFLSNKPLQTRADYAAKLTRLGVPTDADEVINSSLVLARHLATLDPGAPVFVIGEPPL
                     VAELVANGFEVRGDHRVRWVVIAFDRTFDYAKLNTALQAVRRSGARLIATNPDRTCPT
                     EDGEIPDCAGMTAAVEAVTGTSVEVVVGKPSPIILEVALAALGVRAPDAVIVGDRIET
                     DIVMGKRRGLGTVLVLSGITDAADPRIGELRPDLVIGSVGELLQP"
     gene            complement(4162..>4703)
                     /locus_tag="VLF19_11010"
     CDS             complement(4162..>4703)
                     /locus_tag="VLF19_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSE93828.1"
                     /translation="SIAHVGYMLIGLVVGGTAGAAAVLFYLLAYTFTTAGAFGVIALC
                     ERGGVEAVEVRDYAGLGRRYPALALALSLLLLSLVGIPPLAGFVGKFYLFGAAVQAGY
                     IWLAVIAVLNSAVAAYYYLRIIVTMYMHEADGMPARLMPSFAGGLALVIALVGIVLLG
                     VMPAPFADLAQAAVAPLLR"
BASE COUNT          680 a         1637 c         1713 g          673 t
ORIGIN      
        1 cttgccggcg aagacgttct ccatctcgcc ctcgatgatg tcgcggatct gcacgaagtt
       61 gccgaggcgc aggccctggc tgttcggcgt ggcgacccgt tcgggcttcg cggccgggcc
      121 cttcccgctc ttcagcgccg agggtggcgt ggggggaagg cccgtgagct gcgcgaaggc
      181 cgtgtactgc ttgggattgc ggacgaagtg gtacccctcc tcgaggttct tcaccgcggt
      241 gttcgagatg gcgaggtagc cggtgttgac gtgccaccac atctgctgct cgggcctggc
      301 caggtactcg aggaacttgg ccactccccg gtagtccgcc tgcggcttgc ccttgagcac
      361 ccagagtgtg gccccgccga tgatggaggt cgccttcctg tacggcgcgc cccagtgcgg
      421 cagctccgcg gtgccccagt cgaacttcgc cgaacgagtg aacccgccga tgagggccga
      481 ggaggcgaac tgcatggcgc actccccgtt gatgaacttc ggttcggacg ccccgccgcg
      541 ccccccgtag gaaaagatgt gctccttctg ccaggcggcc agcgcgccga tgtgcttctg
      601 acccagctcc cggttgatca gcagctcgac cccctcgatc ccgccgaagc cgttgttctt
      661 ggtggcgaag ggctggtcgt gagtggcgtg aaggttctcg agctggatcc acgacggcca
      721 gctcgacgag aagccacact tggcggcgcc gctggccagc agcttgcggg cggcggcctc
      781 gaggtccttc caggccttcg gcggcttctc gggatcgagc ccggccttct tgaaggcatc
      841 cttgttgtag tagaggatcg gcgtggagga gttgtacggc atcgagtaga gcttgccgtc
      901 cttcgtgtag tagccgacca cgggcttgat gaagtcgttc cagttgatct tgatctcgtt
      961 ctgctgcatc agctcgtaga cggggtacac ggcgccggac agcagcatcg tctgggtccc
     1021 cacctcgaac acctggacga tgtgcggcgg gttcttctgc cggtaggcgg ccaccgcgga
     1081 ggtcagggtc tcggcgtacg agcccttgcg caccaccttg acctcgtagt ccggctggct
     1141 ctggctgaac tcgcgcgcct ggctctcgac ggtctcggcg agctgccccg agatgccgat
     1201 ccacatctgg atgtcggtct tggccagggc gccggccggg gcgagcgcga gcccgaaagc
     1261 cacgacggcg gtcatgccgg tccagcggcg gatgcgcgag tccatgcgag cctcctcaga
     1321 cggagcggtg ccgggactat agcacaggcg tccgtgacgc cccggcgacg tcaccgttcc
     1381 gcgcggaagc gctgcgagcg cgccacgtga gcccggtagg cgtcgagctc ggcgcccagg
     1441 cgccgcgccg tccagccggc gagctgcgcc aggcgctgcc cgatcccttc cgcgcagtcg
     1501 acaccctgac accggctcgt gccgatgccg gtgcggcgca ggagcacgtc ctggagcgac
     1561 acggcgagct cgtcctgcac ggccaggtgg agctgcgcga cgatgtcggg gttctccggg
     1621 cagaggcgct cgccgccgcc tgggatcttg ccggccagct cgatcacgcg ccggtaaccg
     1681 cggccgtaga gggtgaccag cgtctccagg gtctcgcgcc cgaggccgga tgccgccatc
     1741 tccggcgaca cgtcgagcca ggtgcgggcc tcgacgcgcc ccggttcctc gtcggcgccg
     1801 tcgagcgtga gctgccccga gcggctgggc gcgtgccgtc ccagcgcctc gagcacctga
     1861 tcgcccagcc cctcggcgag gctgcggtag caggtgagct tggtcccggt gatcgacagg
     1921 aaggtgccct cggcctcgga gacgacccgg tgcgcccgcg agacgtcgga cgcgcgcttg
     1981 ccctcctcga acgtcagcgg ccgcacaccg gcgtaggtgt aggcgacccg gtcgagggtc
     2041 acgcggggat cgggcacgac cttgcggacc tcgtccagca ggtacgtcac ctcctcgcgg
     2101 gtggcgtgca gccggtccag gtcgccgtcg aagtcggtgt cggtcgaccc cacgagcgaa
     2161 aagccgcgcc acgggatgac gaagatcatg cggtcatccc gcgtgctgtg gtagacggcg
     2221 tggttgctga gacggggcag caggcagtgg atgcccttgg tgcggcgcag gatgcgcggg
     2281 ccgcggtcgg cgacgcccgc gcgctcgcgc acgaggtcca cccacggccc ggtcgcgttc
     2341 acgacgacac gcgcgccgag cgtcgccacg cgcccggtca ggaggtcccg cgccttcacg
     2401 ccgacgacgc gcccgcgcgg gtcacggacg atctcctcca cgtccgcgta gttgaaggcg
     2461 cgggcgccgt ggcggcacgc cgagagcacg ttctccaggc acagccgctc ggggtacacg
     2521 agcagatcgt cgaagtagta gccggagccc ttgaggtcct gggcgcggat gcccggctcg
     2581 aggctcagcg cgtcgacggg cttgagcacg cggtaaggct cgcggtcgcg ccccggcgtg
     2641 agccagtcgt agagcttgag gccgatccgg accttcacga ggccgcgtga cgactcgcgg
     2701 tagacgggca cgaggaacgg caggggccgt acgaggtgcg gcgccagccg gcgcagcgtc
     2761 tcgcgctcgc gcagcgactc gcgcacgagc ccgaaatcga acagctccag gtagcgcaga
     2821 cccccgtgga cgagcttcga ggagcgggag gtcgtgccgg acgcgaagtc gcccttgtcg
     2881 aagagcgcca cggaggcgcc gcgcagcgcg agctcgcgcg ccaccccggc ccctgccatc
     2941 cccccgccga ggatcacgac atcgtaggtc ccgtcggtca gcgggaaggg acggctcatg
     3001 gctggagcag ctctccgacc gatcctatca cgaggtcggg gcgcagctcg ccgatgcgcg
     3061 ggtcggcggc gtccgtgatg ccgctcagca cgagcacggt gccgagacct cgccgcttgc
     3121 ccatcacgat gtcggtctcg atgcggtcgc cgacgatgac cgcgtcgggc gcccgcacgc
     3181 ccagcgcggc cagggccacc tcgaggatga tcggcgacgg cttgccgacg acgacctcca
     3241 cgctcgtccc ggtgaccgcc tcgaccgcgg ccgtcatgcc cgcgcagtcg gggatctccc
     3301 cgtcctcggt ggggcaggtg cggtccgggt tcgtggcgat cagccgcgcg ccgctccgcc
     3361 gcaccgcctg cagcgccgtg ttgagcttgg cgtagtcgaa ggtccggtcg aacgcgatga
     3421 cgacccagcg gacgcggtgg tccccacgca cctcgaagcc gttggcgacg agctcggcaa
     3481 cgagcggcgg ctcgccgatg acgaagacgg gcgcgccggg gtcgagcgtg gccaggtggc
     3541 gcgcgaggac cagcgaggag ttgatcacct cgtcggcgtc cgtgggaacg cccaggcgcg
     3601 tcagcttcgc cgcgtagtcg gcgcgcgtct ggagcggctt gttggacagg aaggctacgc
     3661 gacggccttc ggcgcgtagc gtcgcgaccg tctcggcggc gccggggatc aggacgtcgc
     3721 cacggtagat cgtgccgtcg aggtcgaaga gccaacctcg gtgcggcagg cgtatgcgtg
     3781 gcgtcatccg cgcgtactcc cctcgggcgc gggcgcccgg tctgtccgtc gcgtcgagcg
     3841 ggcgcggggg cctgctgccc ggaacgcgcg atccaccagg cactcgcgcc atgtcatcgg
     3901 gccacgaagg taccgtcggc gtctccggcc cggccaggcc ctcgcgcgcc tcggcgcccg
     3961 ccgcccgccg aggccccggc gtggctcccg tgtacccacg ttcggcacat cccggcgacg
     4021 agcggtgagt ctcgatcgtc tgcgctcacc gcgctcaccg acggcggccc cgggtcgtgc
     4081 cggcctgcgc tcacggcgcg cgcgtggcgc ggtagccgcg gcggcgggtc agcgaccggc
     4141 gcccgccgcg ccgccgggaa ctcaacgaag gaggggggcg acggcggcct gggcgaggtc
     4201 ggcgaagggg gccggcatga cccccaggag gacgatgccg accagagcga tgacgagcgc
     4261 gagcccgccg gcgaacgagg gcatcaggcg ggcgggcatc ccatcggcct cgtgcatgta
     4321 catggtcacg atgatccgca ggtagtagta ggcggcgacc gcggagttca gcacggcgat
     4381 caccgccagc cagatgtatc cggcctgcac cgccgccccg aagaggtaga acttgccgac
     4441 gaagccggcg agcggcggga tgccgaccag cgacaggagc aagaggctca gggccagggc
     4501 cagcgccggg taacggcggc ccaggccggc gtagtcccgc acctcgaccg cctccacgcc
     4561 gccccgctcg cagagggcga tcacgccgaa ggcgccggcg gtcgtgaagg tgtacgccag
     4621 gagatagaag aggaccgcag ccgctcccgc cgtgccgccg accacgaggc cgatcagcat
     4681 gtagccgacg tgcgcgatgg agg
//
LOCUS       DATIAZ010000316         3237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1408766, whole genome shotgun sequence.
ACCESSION   DATIAZ010000316 DATIAZ010000000
VERSION     DATIAZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408766"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..747)
                     /locus_tag="VLF19_11015"
     CDS             complement(61..747)
                     /locus_tag="VLF19_11015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSE93829.1"
                     /translation="MTLAATALLTLLAGCARDRTATLPADRDAPILYVALGDSTVEGV
                     GASGGPATYVARLHERLRAHYPRARVANLGVAGATSADVRAAQVERAVSLRPQLVTLS
                     VGPNDITAGVSVDDYERNVDTVLRRLSEAGRPVVVVNLLPDLAVTPRFAGHPGAATVG
                     RRTVAFNDALRRTVHEHGAGLVDLYGPSRQEVPRQPWLLSADGYHPSDAGYARWAELM
                     WQGVEARLAR"
     gene            complement(762..1709)
                     /locus_tag="VLF19_11020"
     CDS             complement(762..1709)
                     /locus_tag="VLF19_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE93830.1"
                     /translation="MKRAAYVIPGRDLAAAVELARGAEALGYDSVWVTHGLGRDSFLV
                     LAAYGAATRRIGLGNGVVPIYPRHPVAMAQAALTLSEMTDGRFTLGVGVSHRPMIEGM
                     LGLRLDDPLGAMREYVSVLRGALGSGASFVGRHFRVEWSSGFPARPPAPPIYLAALSR
                     RMLELAGEIADGAVLWLCPPAYVRDVALPAIERGRRRTGKDLAGFEIVAAVPLAITTD
                     ATALTVFRAELARYVALPFYRAMMKSSGLGAELAAYDRDKEVPSGLASALGAVGDAAT
                     GRAFLRSYRDAGVTLPAVRPITFPDAPWYRATLEAAAGW"
     gene            complement(1727..1987)
                     /locus_tag="VLF19_11025"
     CDS             complement(1727..1987)
                     /locus_tag="VLF19_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSE93831.1"
                     /translation="MMVVDGEEYLTVDEAARLLEVKRATLYAYVSRGMLRSYRQGMKR
                     QRLYRRAEVEALVRIAPARPDAPAAVRRRSAAIPLAESWIRD"
     gene            2096..>3237
                     /locus_tag="VLF19_11030"
     CDS             2096..>3237
                     /locus_tag="VLF19_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSE93832.1"
                     /translation="MESGWKSGLEDVIAARSAICRVDGEAGRLYYRGYEIADLAGAVE
                     FEDVTWLLWFGELPARAEAEDFRARLAAARALPAPVLGLLRSLPRDSHPLDALRTAVS
                     LAATADADARAGDREANIAKAVRLLTLVPATVAAWHRLRTGREPVAARREGSHAAWFL
                     ESLTGEPPSRGVARVMDLALTLHADHEFNASTFALRVAVATLADLHAAVTAAVATLKG
                     PRHGGANEDVLAMLHEIGDPARAEAYVEARLGRRATLDRAGRGDPRARVPGFGHRVYK
                     VDDARARVLRGMAKSMAEATGRERLFAVAERVYDAMKARTSLPVNVDFFSAVVYDALG
                     IPPDFCTSIFAVGRSAGWCAHALEQFADNRLIRPRADYVGPAPRLLP"
BASE COUNT          434 a         1199 c         1203 g          401 t
ORIGIN      
        1 cagcgtcacc agcgcgatga gtccgtccgt cgtcagcagc gcttccatgc tccactcctc
       61 tcagcgtgcc agccgcgcct ccacgccctg ccacatcagc tccgcccagc gcgcgtaccc
      121 cgcgtcggag gggtggtaac cgtcggcgga gaggagccac ggctgccggg gcacctcctg
      181 gcggctgggg ccgtagaggt ccacgagccc ggcgccgtgc tcgtgcaccg tccggcgcag
      241 ggcgtcgttg aaggccacgg tgcgccggcc gaccgtggcg gctccggggt gccccgcgaa
      301 gcgcggcgtc accgccagat ccggcagcag gttgaccacg acgaccgggc ggccggcctc
      361 cgacagacgg cgaaggaccg tgtccacgtt gcgctcgtag tcatcgaccg acaccccggc
      421 ggtgatgtcg ttcgggccga ccgagagcgt caccagctgg ggccggagcg acacggcgcg
      481 ctccacctgc gccgcgcgga catccgcgga ggtcgcgccg gcgacaccga gattggccac
      541 gcgcgcccgc ggatagtgcg cgcgcagccg ctcgtgcagg cgcgccacgt acgtcgccgg
      601 cccgccgctg gcacccacgc cctcgaccgt gctgtcgccg agcgcgacgt agaggatcgg
      661 cgcgtcgcgg tccgccggga gtgtcgccgt gcggtcgcgc gcgcagccgg cgaggagagt
      721 cagcagcgcg gtggcggcga gggtcatccg ccccggccgc gtcaccagcc tgcggccgcc
      781 tcgagggtcg cgcggtacca cggcgcgtcg gggaaggtga tgggccgcac cgcgggcagc
      841 gtcacgcccg cgtcccggta ggatcgcagg aatgcccgtc ccgtcgcggc gtcgcccacg
      901 gcgccgagcg cgctcgcgag gccggacggc acttccttgt cgcggtcgta cgcggcgagc
      961 tcggcgccga gtccgctcga tttcatcatc gcccggtaga acggcaacgc gacgtagcgc
     1021 gccagctcgg cgcggaagac cgtcagcgcg gtggcgtcgg tcgtgatcgc cagcggcacc
     1081 gccgccacga tctcgaatcc cgcgaggtcc ttgccggtcc ggcggcggcc gcgctcgatg
     1141 gccggcagcg ccacgtcgcg gacgtaggcc ggcgggcaga gccacagcac cgccccgtcg
     1201 gcgatctccc cggccagctc gagcatgcgg cgcgagagcg ccgcgaggta gatcggcggc
     1261 gccggcggcc gcgccgggaa ccccgaactc cactcgacgc ggaagtgccg gcccacgaac
     1321 gaggcgccgg atccgagcgc cccgcgcagc accgagacgt actcgcgcat ggcgccgagg
     1381 ggatcatcga ggcgcaggcc cagcatgccc tcgatcatcg gccggtggct cacgccgacg
     1441 cccagcgtga agcgcccgtc cgtcatctcc gagagggtca gcgccgcctg ggccatggcc
     1501 accgggtggc gggggtagat cggcacgacg ccattgccca gaccgatccg gcgggtggcc
     1561 gcgccgtagg cggccagcac gaggaacgag tcgcggccga ggccgtgggt gacccagacg
     1621 ctgtcgtagc cgagggcctc ggcgccgcga gccagctcga cggcggcggc gagatcgcgt
     1681 cccgggatga cgtaggcggc gcgcttcatg acgtcgaggg cggtggtcag tcgcggatcc
     1741 aggattcggc gagcggaatc gctgctgagc ggcggcgcac ggcggccggg gcatcgggcc
     1801 gggccggcgc gatccgcacg agcgcttcga cctcggcacg ccggtagagc cgctgccgct
     1861 tcatgccctg gcggtagctc cggagcatgc cccggctcac gtaggcatag agggtcgcgc
     1921 gctttacctc gagcagccgc gccgcctcgt cgaccgtcag gtactcctcg ccgtccacca
     1981 ccatcatcgt ccccaccgta gcaggcgccc tcgggccggt ctagtctaat caatatgaat
     2041 caagtaaaat catgtcgagt aaacaaaggc ccggatggcc gggcgggagg ggcccatgga
     2101 aagcgggtgg aagtcggggc tcgaggacgt gatcgcggcg cggtcggcga tctgccgagt
     2161 ggacggcgag gcgggacggc tctactaccg cggctacgag atcgccgacc tggccggggc
     2221 cgtcgagttc gaggacgtga cgtggctcct gtggttcggc gagctgccgg cgcgcgccga
     2281 ggccgaggac ttccgcgcgc ggctcgccgc ggcgcgcgcg ctgccggcgc ccgtgctggg
     2341 cctgctccgg tcgctgccgc gcgacagcca tccgctcgat gcgctgcgca ccgccgtctc
     2401 gctggcggcg accgccgacg ccgatgcgcg ggcgggagat cgcgaggcga acatcgcgaa
     2461 ggccgtccgg ctcctgaccc tggtgccggc gaccgtcgcc gcgtggcacc gcctgcgcac
     2521 cgggcgcgag cccgtggcgg cgcgccgcga gggctcccac gccgcgtggt tcctcgagtc
     2581 gctcaccggc gagccaccct cgcgcggcgt cgcgcgcgtc atggacctcg cactcacgct
     2641 gcacgccgat cacgagttca acgcctcgac gttcgcgctg cgcgtggccg tggccacgct
     2701 cgccgacctg cacgccgcgg tgacggccgc cgtggccacg ctcaagggac cgcgccacgg
     2761 cggcgccaac gaggacgtgc tcgccatgct gcacgagatc ggcgatcccg cccgcgcgga
     2821 ggcgtacgtg gaggcccggc tcggccggcg cgcgacgctc gaccgtgccg gacggggcga
     2881 tccccgggcc cgcgtgccgg ggttcgggca ccgcgtctac aaggtcgacg acgcgcgggc
     2941 ccgcgtgctg cgcggcatgg cgaagtcgat ggccgaggcg accggccggg aacggctctt
     3001 cgcggtggcc gaacgcgtct acgacgcgat gaaggcgcgc acgagcctgc ccgtcaacgt
     3061 cgacttcttc tcggccgtcg tctacgacgc gctcggcatc ccgccggact tctgcacgtc
     3121 gatcttcgcc gtggggcggt cggccgggtg gtgcgcccac gcgctcgagc aattcgccga
     3181 caaccgtctc atccggccgc gcgcggacta cgtcggcccg gcgccgaggc tgttgcc
//
LOCUS       DATIAZ010000317         7793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_223264, whole genome shotgun sequence.
ACCESSION   DATIAZ010000317 DATIAZ010000000
VERSION     DATIAZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223264"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VLF19_11035"
     CDS             complement(<1..642)
                     /locus_tag="VLF19_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSE93833.1"
                     /translation="MSDAGGVKVDVQERGARGQSSSRRLYMQLQVFGGCADPKPLVHA
                     LEGGRAEAVLYADLNDPRGVGLVTMSEDPAFFVGAGRDLLNAEPFAGLAHRPALTMFG
                     RTYASGFEADLDEWLLRRPRRTALNPAWPWAVWYPLRRTGGFARLPGQEQGTIIREHS
                     VLGRAYGDADLAHDIRLACHGLDVHDNDFVIGLVGRDLHPLSHLVQTMRKTVQT"
     gene            complement(644..1672)
                     /gene="moaA"
                     /locus_tag="VLF19_11040"
     CDS             complement(644..1672)
                     /gene="moaA"
                     /locus_tag="VLF19_11040"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSE93834.1"
                     /translation="MTAIHDAYGRVLRNLRISVTDRCNLRCQYCMPEEDYAWLPRDEL
                     LHFEEIADLVDIFIGLGVDKVRLTGGEPLLRRDLPRLVRLLAARSGLRDLAVTTNGLL
                     LAAQATALRDAGLHRVTVSLDTLRAARFQALTRRTGLEQVLEGIAAAPRAGFAGTKID
                     TVAMRGVNDDELVDLVEFAKGVPAEIRFIEYMDVGGATRWSIDRVVSRREILDVLGRR
                     YGAIEPVPEPGSTAPAERFRLPDGTVFGIIASTTAPFCAACDRSRLTADGMWYLCLYA
                     LQGTDLRGPLRRGASAAELAALVTRGWRARTDRGAEERLAARDRGPLVQIARLRQDPH
                     LEMHTRGG"
     gene            complement(1669..3381)
                     /locus_tag="VLF19_11045"
     CDS             complement(1669..3381)
                     /locus_tag="VLF19_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSE93835.1"
                     /translation="MSWILKRKARAWLAHEQGTICKDWGGRVAIALVYPNSYSVGMSN
                     LGFQTIYWRLNQLPDVVCERVFLPDPEDVDEMRRTGTLPFSLESQRSLADFHMVGFSV
                     TYEGDYVNALRLLALAGIPLRAAERRPRDPLVLMGGVCAFSNPEPMAPFMDFIVVGEG
                     EEVVEELVQAYRDGSDDRARLLDALARIPGVYIPSRYDVSYQPDGTLAAVLPRDGAPA
                     VVIKRRLRDVDRFETISLVKTPDAEYGHMALLEVGKGCGRGCRFCLEGQVYRPVRHRS
                     VATLGETIGRIAADPVNRRIGLVGACVSDYPWIGELLKIVEERGLELSISSLRADSLT
                     PELVTALARGGHRTLTVAPEAGTERLRRAIRKAISDEQLLAACDLVRRAGIPNLKTYF
                     MIGQPTETPEDVEAIADLARRMLERLRVLDPQGRPFGRLTLSISSFVPKPWTPFQWAP
                     FDGAGSLGTKLETIKHGVRRFSNVRVLHENPREAALQALLARGDRRVGDFLEGAARLD
                     GDWRRALREWEGDPEFYTTRERSVAERLPWDHFDVGVKKTGLLREWERAQSERAPASP
                     AATV"
     gene            complement(3544..4335)
                     /gene="lgt"
                     /locus_tag="VLF19_11050"
     CDS             complement(3544..4335)
                     /gene="lgt"
                     /locus_tag="VLF19_11050"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSE93836.1"
                     /translation="MFASPGAVALQLGPVVIRWYGILMAASIVIGLWLGHRQARREGL
                     PADDIISAGQWAVLAGLVGARVYEVVFNWDYYGQYPSKILAVWEGGLAIHGSLIAGPL
                     VGAWLAYRWKLPVLRGLDVAAPSIAIGQAIGRWGNFFNEEAFGRPTDLPWKLYISPSH
                     RPPGYGQYEFFHPTFLYESLWDLGVFLALILWLRPRMRANPGALFFAYIGLYSVGRFA
                     IEALRMDSFWVGTYRVPQLASVVGVVVALIGVAWTRRRAAAVAAS"
     gene            4373..5017
                     /locus_tag="VLF19_11055"
     CDS             4373..5017
                     /locus_tag="VLF19_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSE93837.1"
                     /translation="MNKRRWRRAGVLVLAPAALALAAITGVCALGRYLVVADTLQPSD
                     AIIVLDGRTPARELEAAALYHQGLAPRVVVARARDPYEVARALAGEPNPQVRALRVLT
                     RLKVPRTAIEALDRVVENTTDELAADFEHARSRGFGRVILVTSPAHTRRVRVIWNARY
                     QAAVPALVHPTPYDPFDTRGWWRSRRSIEYALHEIAGILHFRVGAPLPTFDHTW"
     gene            complement(5187..5272)
                     /locus_tag="VLF19_11060"
     tRNA            complement(5187..5272)
                     /locus_tag="VLF19_11060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5237..5239),aa:Leu,seq:taa)
     gene            complement(5314..5389)
                     /locus_tag="VLF19_11065"
     tRNA            complement(5314..5389)
                     /locus_tag="VLF19_11065"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5354..5356),aa:Cys,seq:gca)
     gene            complement(5425..5499)
                     /locus_tag="VLF19_11070"
     tRNA            complement(5425..5499)
                     /locus_tag="VLF19_11070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5465..5467),aa:Gly,seq:gcc)
     gene            complement(5555..6553)
                     /gene="galT"
                     /locus_tag="VLF19_11075"
     CDS             complement(5555..6553)
                     /gene="galT"
                     /locus_tag="VLF19_11075"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSE93838.1"
                     /translation="MSDLRKDPVVGRWVIIAGERGRRPTDFGPEPTRPRLASCVFCAG
                     NEDKTPPEILAGRSPDSLPNAPGWNYRVVANKFPALRIEGELEPTGEGPFDRMNGIGA
                     HEVVIESPEHEASLATMALDAVADVLLAFRERLLDLKKDPRFEYVLVFKNHGEAAGAS
                     LEHPHSQLIATPIIPIMVSEELMGSETYYAMKERCVWCDVLREERRLRRRLVLERDGF
                     VALAPFAPRFPFETWILPVRHRSAFEESGVDELRALAAILGEFLRRMNRTLNDPPFNF
                     MLHSAPLQAPALEHFHWHLEVIPKLTKVAGFEWGSGFFINPVAPEDAAAALREAPR"
     gene            complement(6567..7562)
                     /locus_tag="VLF19_11080"
     CDS             complement(6567..7562)
                     /locus_tag="VLF19_11080"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSE93839.1"
                     /translation="MARRIGVVGGGSWGTGLAIHLARAGAVVRLWAREPEVVEGIRRA
                     RRNPFYLKDIDCPAGIDPTDDPAEVVRDADVVLLVVPSEFFALTLKGLGRIRRGVPIV
                     SATKGLDPERHVRMSEIVAEQHPDAPVAALSGPSFAREVALGQPTACVVAAADDVLAA
                     RLQAALGTREFRLYTNRDVVGVEMGGALKNVIAIATGIADGLGLGENARAALITRGLA
                     EITRLAVALGAQARTLAGLAGMGDLVLTCTGTLSRNRGLGMALARGESQAAVEGATRM
                     IAEGARTVASALVLAERRGVSLPVCTEVAAVLFDGKPPREALASLLARAARPEEG"
BASE COUNT         1202 a         2800 c         2733 g         1058 t
ORIGIN      
        1 ggtctgcacg gtcttccgca tcgtctgcac caggtgcgag agcgggtgca ggtcgcgccc
       61 gacgagcccg atgacgaagt cgttgtcgtg gacgtccagg ccgtgacagg cgaggcggat
      121 gtcgtgggcg aggtcggcgt ctccgtacgc ccgcccgagc acactgtgct cgcgaatgat
      181 cgtcccctgc tcctgacccg gcaggcgcgc gaagccgccc gtccgtcgca gcggatacca
      241 cacggcccac ggccaggccg ggttgagcgc ggtgcgccgc gggcgccgga gcagccactc
      301 gtccagatcg gcctcgaagc ccgaggcgta cgtccggccg aacatcgtga gcgccgggcg
      361 gtgcgccagt cccgcgaagg gctcggcatt gagcagatcg cgccccgcgc ccacgaagaa
      421 ggccgggtcc tcgctcatcg tcaccaggcc gacgccgcgc ggatcgttga ggtcggcgta
      481 gagcacggcc tccgcgcgtc cgccctcgag tgcgtgaacc aggggcttgg gatcggcgca
      541 gccaccgaag acctggagct gcatgtagag acgacggctg gagctctggc cccgcgcgcc
      601 gcgctcctgg acgtcgacct tgacgcctcc ggcgtcgctc acgtcagcct cctcgcgtgt
      661 gcatctccag gtgcgggtcc tggcggagcc gcgcgatctg aacgagcggc ccgcgatcgc
      721 gcgcggccag gcgctcctcg gcgccgcggt ccgtgcgggc ccgccagccc cgggtgacga
      781 gggccgcgag ctccgcggcg ctcgccccac ggcgcagagg cccgcgcaga tcggtcccct
      841 ggagcgcgta gaggcagagg taccacatgc cgtcggcggt gaggcggctc cggtcgcacg
      901 cggcgcagaa cggcgccgtg gtggacgcga tgataccgaa caccgtgccg tcggggagcc
      961 gaaaccgctc ggccggcgcc gtgctgccgg gctccggcac gggctcgatc gcgccgtagc
     1021 ggcgcccgag cacgtccagg atctcgcggc gcgacacgac ccggtcgatc gaccagcgcg
     1081 tggcgccgcc cacgtccatg tactcgatga agcggatctc cgccggcacg cccttggcga
     1141 actccacgag gtcgacgagc tcgtcgtcgt tcacgccgcg catggccacc gtgtcgatct
     1201 tggtcccggc gaacccggcg cgcggcgccg cggcgatccc ctccagcacc tgctcgaggc
     1261 ccgtccgtcg ggtcagtgcc tggaagcggg ccgcgcgcag tgtgtcgagg ctcacggtga
     1321 cccggtgcag tcccgcgtcg cgaagcgccg tcgcctgcgc cgcgagcagg aggccattgg
     1381 tggtgaccgc caggtcgcga agaccggacc tggcggccag cagccgcacg agccgcggga
     1441 ggtcacggcg cagcagtggc tcaccgcccg tcagccgcac cttgtcgacg ccgaggccga
     1501 tgaagatatc caccaggtcg gcgatctcct cgaagtggag cagctcgtcg cgcggcagcc
     1561 acgcgtagtc ctcctccggc atgcagtact ggcagcgcag gttgcaccgg tcggtgaccg
     1621 agatgcggag gttcctgagg acgcggccgt aggcgtcgtg gatggccgtc ataccgtcgc
     1681 cgcgggcgag gccggcgcgc gctcggactg cgcccgttcc cattcgcgga gcagacccgt
     1741 cttcttgacc ccgacgtcga agtggtccca cggcaggcgc tcggccaccg atcgctcccg
     1801 ggtggtgtag aactccggat cgccctccca ctccctgagc gcccgccgcc agtcgccgtc
     1861 caggcgcgcc gcgccttcga ggaagtcgcc gacccgccgg tcgccgcgcg ccaggagcgc
     1921 ctgcagcgcc gcctcgcgcg gattctcgtg gagcacgcgc acgttggaga accgccggac
     1981 gccgtgcttg atcgtctcga gcttggtccc gagcgacccg gccccgtcga agggggccca
     2041 ctggaacggg gtccagggct tgggcacgaa cgacgagatc gacagcgtga gccggccgaa
     2101 gggacgtccc tgcgggtcga gcacgcggag ccgctcgagc atgcggcgcg ccagatcggc
     2161 gatggcctcg acatcctccg gcgtctcggt cggctggcct atcatgaagt acgtcttgag
     2221 attggggatc cccgcgcggc gcacgaggtc gcacgccgcg aggagctgct cgtcgctgat
     2281 ggccttgcgg atggcgcggc gcagccgctc ggtcccggcc tcgggcgcga ccgtgagtgt
     2341 ccggtgaccg ccgcgggcca gcgccgtgac cagctcgggc gtcaggctgt cggcccgcag
     2401 cgacgagatg gaaagctcga gtccccgctc ctcgacgatc ttcagcagct cgccgatcca
     2461 cggatagtcc gagacgcacg ccccgaccag gccgatgcgc cggttgacag gatcggcggc
     2521 gatccggccg atcgtctcgc cgagcgtggc aacggaacgg tgacgcaccg gccggtaaac
     2581 ctggccctcg aggcagaagc ggcagccacg gccgcaaccc ttgcccactt cgagcagcgc
     2641 catgtgaccg tactcggcgt ccggcgtctt gacgagcgag atcgtctcga accggtccac
     2701 gtcgcggagc cgccgcttga tgacgacggc cggcgcgccg tcgcgcggca gcacggccgc
     2761 gagagtcccg tcgggctggt acgacacgtc gtaccgcgag ggtatgtaca cgccggggat
     2821 ccgagcgagg gcgtcgagaa gacgcgcccg gtcgtcgctc ccgtcgcggt acgcctgcac
     2881 cagctcctcg acgacctcct ctccctcccc cacgacgatg aagtccatga agggcgccat
     2941 cggctcgggg ttggagaagg cacagacgcc gcccatgagc acgagcgggt cgcgcgggcg
     3001 gcgctcggcc gcgcgcaagg gaatgccggc cagggcgagc agacgaagcg cattgacgta
     3061 atcgccctcg taggtgacgg aaaagccgac catgtggaag tccgcgagcg accgctggga
     3121 ctccagcgag aagggcagcg tgcccgtgcg gcgcatctcg tccacgtcct cgggatcggg
     3181 caagaacacg cgctcgcaga cgacgtcggg caactggttg aggcgccagt atatcgtctg
     3241 gaagccgagg ttcgacatcc cgaccgagta ggagttcggg tagaccagcg cgatggcgac
     3301 gcggccgccc cagtccttgc agatcgtccc ctgctcgtga gcgagccacg cccgggcttt
     3361 tcgcttcaag atccaggaca tcggggtcag tctaccatgg gctgtctcgg cccttgatga
     3421 cgccgatcgg acagaggcgg acgacgcgac gggagatgcc gacggcgtgg accacgttca
     3481 cgacgtcttc gacgtcctgt aggcctccag catctcctcc gccaggcggg aattcaccgg
     3541 acatcagctc gcggcgacgg ccgcggctcg ccgacgcgtc cacgccacgc cgatcagagc
     3601 caccaccacg cccacgacgc tggcgagctg gggcactcga taggtgccga cccagaagct
     3661 atccatccgg agcgcctcga tcgcgaagcg gcccaccgag tacagcccga tgtaggcgaa
     3721 gaacagcgcg ccggggttgg cgcgcatccg cgggcgtagc caaaggatca gggcgaggaa
     3781 cacgccgagg tcccacaggg actcgtacag gaaggtgggg tggaagaatt cgtactggcc
     3841 gtagcccggc ggccggtgcg acggcgagat gtagagcttc cagggcaggt cggtcgggcg
     3901 gccgaacgcc tcctcgttga agaagttccc ccagcgcccg atcgcctgcc cgatggcgat
     3961 gctcggggct gcgacgtcga gcccccgcag caccggcagc ttccagcgat acgcgagcca
     4021 ggcgccgacc agcgggccgg cgatcaacga gccgtggatg gcgagcccgc cctcccagac
     4081 cgcgaggatc ttggatgggt actggccgta gtagtcccag ttgaagacga cctcgtagac
     4141 gcgcgcgccg acgagcccgg cgaggaccgc ccactgccct gcgctgatga tgtcgtcggc
     4201 cggcaggccc tcgcgccgcg cctgacggtg cccgagccat agaccgatga cgatcgacgc
     4261 cgccatgagg atgccatacc agcggatgac gacggggccg agctgcaggg cgacggcgcc
     4321 gggggaggcg aacatctcgg gtaagattat ctcgacggat ggcccacgtc caatgaacaa
     4381 gcgccgttgg cgccgcgccg gggtgctcgt gctcgccccg gcagccctgg cgctcgccgc
     4441 catcacgggc gtttgcgcgc tgggtcgcta cctggtggtt gccgacaccc tccagccgag
     4501 tgacgcgatc atcgtgctgg acggcaggac cccggcgcgc gagctggagg ccgcagcgct
     4561 gtaccaccag gggctcgcgc cgcgcgtggt cgtcgcccgc gcgcgcgatc cctacgaggt
     4621 cgctcgggcg ctcgctggag agccgaatcc ccaggtaagg gctctgcgcg tgctgacccg
     4681 cctgaaggtg ccccgcaccg cgatcgaggc cctcgaccgg gtcgtggaga acacgacgga
     4741 cgagctggcc gccgacttcg agcacgcgcg atcgcgcggc ttcggacggg tgatactggt
     4801 gacctcgcct gcccacacca ggcgcgtccg ggtgatctgg aacgcgcgct atcaggccgc
     4861 ggtgccggcc ctcgtccacc cgacgccgta tgacccgttc gacacccgcg gttggtggcg
     4921 ctcgcgccgg tccatcgaat acgccctgca cgagatcgcc ggcatcctgc acttcagggt
     4981 cggcgccccg ctgccgacgt tcgaccacac gtggtagcgc gcgacgcact gcctcacgtc
     5041 gcgccggacc tcgctgccgg gcgggcagcc gaggatgcgg tcgacacggg ccacatcggc
     5101 gcggcgggcg aggagatggt caggacgttc gaccgacggg tgaggatggc ggcgaggtca
     5161 cacgccctac gaacagcgcg aagaattggt aggggcggca ggattcgaac ctgcaagtcc
     5221 tcgcggacca aggcttttaa ggcctctacg ttttccgttc cgtcacgccc ccaggttccc
     5281 tgcgtggctt gcgaagagcc ctcgagaagg aagtggaggc ggcgagcgga ttcgaaccgc
     5341 tgaatagagg ttttgcagac ctccgcctta accacttggc tacgccgccc tggcaaaacg
     5401 agaacgggtc gaggcgagga gggctggagc gggaaacggg attcgaaccc gcgaccccga
     5461 ccttggcaag gtcgtgctct accactgagc tattcccgca tccctgaccc gccgccgcga
     5521 cagcgtccta tggtaccacg cccgtctgac ccgtctacct cggcgcctcg cgcaaggccg
     5581 ccgcggcatc ctccggcgcg acggggttga tgaagaagcc gctgccccac tcgaagcccg
     5641 ccaccttcgt gagcttcggg atgacctcga ggtgccagtg gaagtgctcc agggcgggcg
     5701 cctggagcgg cgcgctgtgg agcatgaagt tgaagggcgg atcgttcagg gtccggttca
     5761 tccgccgcag gaactctccg aggatcgcgg ccagcgcccg cagctcgtcc acccccgact
     5821 cctcgaacgc cgagcggtgg cgcaccggca ggatccacgt ctcgaacggg aaccgtggtg
     5881 cgaacggggc gagcgccacg aacccgtctc gctcgaggac gaggcgccga cgcaagcgac
     5941 gctcctcgcg caggacgtcg caccagacgc agcgctcctt catggcgtag tacgtctcgg
     6001 agcccatcag ctcctcgctc accatgatcg ggatgatcgg ggtcgcaatg agctgcgagt
     6061 gggggtgctc gagcgacgcc ccggccgcct cgccgtgatt cttgaacacc aggacgtact
     6121 cgaagcgcgg gtccttcttg aggtcgagca ggcgctcgcg gaaggcgagc agcacgtcgg
     6181 cgaccgcgtc cagcgccatc gtcgccagcg aggcctcgtg ctccggcgat tcgatgacca
     6241 cctcgtgggc gccgatgccg ttcatccggt cgaaggggcc ctcccccgtc ggctcgagct
     6301 ccccctcgat ccgcagcgcg gggaacttgt tggcgacgac gcgataattc caccccggcg
     6361 cgttcggcag actgtcaggg ctgcggccgg ccaggatctc gggcggcgtc ttgtcctcgt
     6421 tgcccgcgca gaacacgcac gaggccaggc gcggccgggt gggctccggg ccgaaatcgg
     6481 tcggccgccg cccacgctct ccggcgatga tcacccaccg cccgaccacc ggatccttcc
     6541 tcaggtccga catcccgtcc cttgcctcac ccctcctcgg gccgcgccgc ccgggcgagc
     6601 agcgaggcga gcgcctcgcg cggcggcttg ccgtcgaaga gcacggccgc cacctcggtg
     6661 cagacgggga ggctcacacc ccgccgctcg gcgagcacga gcgccgaggc taccgtccgc
     6721 gcgccctccg cgatcatgcg ggtggcgccc tccaccgccg cctggctctc gccgcgcgcc
     6781 agggccatcc cgaggccgcg gttgcgcgag agcgtgcccg tgcacgtcag cacgagatcc
     6841 cccatgcccg cgagccccgc cagcgtgcgc gcctgagcgc ccagcgcgac ggcgaggcgg
     6901 gtgatctcgg ccaggccccg ggtgatgagc gccgcgcgcg cgttctcgcc gagcccgagt
     6961 ccgtcggcga tgcccgtcgc gatcgcgatg acgttcttga gcgcgccccc catctcgacg
     7021 ccaacgacgt cgcggttggt gtagaggcgg aactcccggg tgcccagcgc ggcctgcagg
     7081 cgcgcggcca ggacgtcatc cgcggcggcg acgacacagg ccgtcggctg gccgagagcc
     7141 acctcgcgcg cgaacgacgg gcccgacagc gcggccaccg gcgcgtccgg atgctgttcg
     7201 gcgacgatct cgctcatgcg cacgtggcgc tccggatcga gacccttcgt ggcggagacg
     7261 atcgggacgc cacggcggat ccgccccagc cccttgaggg tgagcgcgaa gaactccgac
     7321 ggcaccacga ggagcacgac gtcggcatca cgcacgacct ctgcgggatc gtccgtcggg
     7381 tcgatgccgg ccggacagtc gatgtccttc aggtagaacg ggttgcggcg ggccctccgg
     7441 atgccctcga cgacctcggg ctcgcgggcc cagaggcgca ccacggcacc cgcccgcgcc
     7501 aagtggatgg cgaggccggt gccccagctg ccgccgccga ccacgccgat gcgacgcgcc
     7561 acctcagcgg ccaccggcgc gaccgcccag gagcacggcg ccgtgccgag gcgcgctaca
     7621 cctccgtggc gacgcgccgc gcctcgaggg gcatggaggc ccatgtcgag gccccccgtg
     7681 tcttgatcgg cccatgtcgg ggcccctcat gcagtgagct tcacgcgcgt cgttcgccga
     7741 gacggcgctc cgtcccggcg aagagccgcc cgatgttctc gtggtgacgc agg
//
LOCUS       DATIAZ010000318         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_223377, whole genome shotgun sequence.
ACCESSION   DATIAZ010000318 DATIAZ010000000
VERSION     DATIAZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223377"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1007
                     /locus_tag="VLF19_11085"
     CDS             24..1007
                     /locus_tag="VLF19_11085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSE93840.1"
                     /translation="MRWLVVVPTERPGLMGMDFAAELAALGHEVATFAYRRTNPFYKN
                     RGSKALYQRLLCRRLERRCRSWRPDVVLVIKGGPLAPDFVRRLRTRHGAVVVNLFPDN
                     PLLMIPFECIEAYDLFFSKERYALRSLQQAGLRNLHYLPMYCSPSDHHPVTLTDEERV
                     HFAAPLSFVGSRYPYRERFVRELAGYPLRLWGAGWPRATQPDVRAMAAGGPVYGRDKL
                     CVYSGSTLSLNQHHPMNDIVGLNTRTFELAASGACQVVDFKEDLGTLFKPGEEVLAYR
                     DLVELRRLLDHYLARPDEARAVGDNARRRALAEHTVRHRAEEMLAVIRARA"
     gene            1004..2227
                     /locus_tag="VLF19_11090"
     CDS             1004..2227
                     /locus_tag="VLF19_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSE93841.1"
                     /translation="MKDSLARDLACIECGAVHPLGYRLACDRCRGLLELRYDLDVLRR
                     HGPALLEGEGFWRYAPVLPIADPAHRVSLGEGATPLLDCPRLAARLGVRRLLLKFEGA
                     NPTGTVKDRTSATAVSAAVQFGFKATSVVSTGNAGSSIAAYSARAGLRSFVFAYERAS
                     APKLLHMAATASDLILYKGVYDDLIGHWDRLVEERLFFDCGASRNPYKQEGKKTLAYE
                     IAEQCAWRPPDVVVAPVAVGEVFIAAARGFAELRDAGWIPAMPSMVAAQAARSNPVAR
                     AFESRAPLAPVKIGYTVAEGLACGDPGAKGAWVLRLLRERGGVAGDAEDAEILEAQRL
                     LARTEGVWAGPTGVASLAVLGRLLADKRLDPYQTICAIISETGLKTEAEPPSRAATTF
                     DFPSLRRLLLDRLGG"
     gene            2224..2832
                     /locus_tag="VLF19_11095"
     CDS             2224..2832
                     /locus_tag="VLF19_11095"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSE93842.1"
                     /translation="MTLPRRFYLRPAPTVARDLLGCVLVSRIGRALTAGRIVETEAYL
                     GPEDAASHAAFRPGSRALFYGDGGHAYVYLNYGLHHCLNAIVERAGRPGCVLIRALEP
                     VAGLAVMARRRGVPADSPRLTSGPGNLTRALGITARENGADLRRGRLVIGPPEAPREF
                     RVARGPRVGITRATSRRLRFWVDGSAHVSRAVRPVAERAGPA"
BASE COUNT          402 a         1156 c         1061 g          437 t
ORIGIN      
        1 ggccgtgtgc taggctacga gccatgcgct ggctcgtggt cgttcccacc gagcgaccgg
       61 ggctgatggg aatggacttc gccgcagagc tcgccgcgct cggccacgag gtcgcgacgt
      121 tcgcctaccg gcgcaccaat cccttctaca agaacagggg cagcaaggca ctctaccagc
      181 gcctcctgtg ccgccggctc gagcggcgct gccgatcctg gcggcccgac gtggtgctgg
      241 tgatcaaggg gggcccgctg gcgcccgact tcgtccgccg tctccggaca cggcacggtg
      301 ccgtcgtcgt caacctcttc cccgacaatc cgctgctgat gatcccgttc gagtgtatcg
      361 aggcctacga cctgttcttc tccaaggagc gatacgcgct ccgcagcctc cagcaggccg
      421 gcctccggaa cctccactac ctgccgatgt actgctcgcc gtcggaccat cacccggtca
      481 cgctcaccga cgaagagcgc gtccacttcg cggcgccgct gtccttcgtc ggcagccgct
      541 atccttaccg cgagcgcttc gtgcgcgagc tggcgggcta tccgctgcgg ctctggggcg
      601 ccggctggcc acgcgcgacg caaccggacg tccgcgcgat ggccgccggc ggtcccgtgt
      661 acggccgcga caagctctgc gtgtactccg gctcgacgct ctcgctgaac cagcatcacc
      721 cgatgaacga catcgttggc ctcaacacgc gcaccttcga gctggcggcc tcgggggcct
      781 gccaggtcgt cgatttcaag gaggacctcg gcacgctgtt caagcccggc gaggaagtgc
      841 tcgcctaccg cgacctcgtc gagctcagac gcctgctcga ccactacctg gcgcgccccg
      901 acgaggcgcg cgcggtgggc gacaacgccc gacgccgcgc gctggccgag cacaccgtgc
      961 gccatcgagc ggaagagatg ctcgccgtca tccgggcgcg cgcatgaagg actcgctggc
     1021 ccgcgacctg gcctgcatcg agtgcggcgc cgtccacccg ctcggctacc gcctcgcctg
     1081 tgaccggtgc cggggcctgc tggagctgcg ctacgacctc gacgtgctgc gtcgccatgg
     1141 tccggccctg ctcgagggcg aagggttctg gcgctacgct ccggtcctgc cgatcgccga
     1201 tcccgcgcac cgcgtctcgc tcggcgaggg tgcgacaccg ctgctcgact gcccgcgcct
     1261 cgccgcgcgg ctcggcgtcc ggcgtctgct gttgaagttc gagggcgcca atccgacggg
     1321 caccgtgaaa gaccgcacct cggccaccgc ggtgtcggcc gcggtccagt tcgggttcaa
     1381 ggcgacctcg gtggtcagca ccggcaacgc cggctcgtcc atcgcggcct attcggcgcg
     1441 ggcggggctc cggtcgttcg tgttcgcgta cgagcgcgcc tcggcgccga agctcctgca
     1501 catggccgcg acggcgtcgg acctgatcct ctacaagggc gtgtacgacg acctgatcgg
     1561 ccactgggat cggctcgtcg aggagcggct gttcttcgac tgcggcgcat cccgcaatcc
     1621 gtacaagcag gaaggcaaga aaacgctcgc ctacgagatc gccgagcagt gcgcgtggcg
     1681 gccgccggac gtcgtcgtgg cgccggtcgc cgtcggcgag gtgttcatcg ccgccgcgcg
     1741 cggctttgcc gagctccgag acgccggctg gatcccggcg atgccgtcca tggtcgccgc
     1801 gcaggccgcg cgatccaacc cggtggcgcg cgctttcgag tcgcgagcgc cgctggcccc
     1861 ggtgaagatc ggctacaccg tcgccgaggg cctggcgtgc ggggaccccg gcgccaaggg
     1921 cgcctgggtg ctgcgcctcc tgcgcgagcg gggcggtgtc gccggcgacg ccgaggacgc
     1981 ggagatcctc gaggcccagc gcctcctggc ccggaccgag ggcgtctggg cgggaccgac
     2041 gggggtggcc agcctcgcgg tgctcggcag gctcctggcc gacaagcgtc tcgatcccta
     2101 ccagacgatc tgcgcgatca tcagcgagac cggcctcaag accgaggccg agccgccgag
     2161 ccgcgcggcc acgacgttcg acttcccctc gctccgccgc ctcctcctcg accgtctcgg
     2221 cgggtgacgc tgccgcgccg cttctacctg cgaccggcgc ccaccgtggc gcgcgacctc
     2281 ctcggctgcg tgctcgtctc gcgcatcggg cgcgcgctca cggcgggacg gatcgtggag
     2341 acggaagcct acctggggcc cgaggacgcg gcctcgcacg cggcgttccg tcccggcagc
     2401 cgcgcgctct tctacggcga cggcggccac gcctacgttt acctcaacta cggcctgcac
     2461 cactgcctca acgcgatcgt ggagcgcgcc ggacggccgg ggtgcgtgct catccgcgca
     2521 ctcgagcccg tggccggcct cgccgtgatg gcgcgccgcc gcggcgtccc ggcggactct
     2581 ccccggctga ccagtggccc aggcaacctg acccgcgcgc tcggcatcac cgcgcgcgag
     2641 aacggcgccg acctcaggcg cggccgtctc gtgatcggac caccggaggc cccgcgcgag
     2701 ttccgcgtcg cgcgtggccc gcgggtggga atcacccggg cgacgagtcg gcggctgcgg
     2761 ttctgggtcg acggcagcgc gcacgtatcg agggcggtcc gaccggtcgc ggagagagcc
     2821 ggaccggcgt aggacgacga ccacctgagc ggccgcctcg cccagcgcct ccgggtccaa
     2881 cagcgcgtcg ggcagccccg ggtccatggc gcgatcgggg cgcgacgatg atgggcccgg
     2941 gacgtgtccg gcctcgcgcc gaccaagaac cgggcgcccg tgggaacgcc tcgaccgccc
     3001 cccccacacc gtcggcctcg gccacggggg tagcgccgcc aggagcagga ccgtcg
//
LOCUS       DATIAZ010000319         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_883056, whole genome shotgun sequence.
ACCESSION   DATIAZ010000319 DATIAZ010000000
VERSION     DATIAZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883056"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..117)
                     /locus_tag="VLF19_11100"
                     /pseudo
     CDS             complement(1..117)
                     /locus_tag="VLF19_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            237..1577
                     /locus_tag="VLF19_11105"
     CDS             237..1577
                     /locus_tag="VLF19_11105"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HSE93843.1"
                     /translation="MADHAPFLPGLSPVGGKPVHVAFDGGRLTSDAGVLVLAEIERRL
                     GLAERLARCIEDPRTPERVRHGLAEMIRFRALMIAAGYADANDCDALRADPAFKLAVG
                     RLPETGADLCSQPTMSRLENLPGATALKRMMAAMVELFCNSFDEVPRRIVLDIDDTED
                     RVHGRQQLALFHAHYDSRCFLPIHVYEARSGKPVAVILRPGKTPDGAEVALVLRHVVR
                     AIRSHWPAVDILIRGDSHYGRPEAMSWLERNRVGYIFGLAGNRVLLARVGHVAEGAAL
                     DRVAGGADKIRRYAEFHYGAKTWPAERQVIARVEASAQGSDSRFVVTNLKGTPRWLYE
                     AVYCARGQAENLIKAHKLHLASDRTSCSKATANQFRLLVHTAAYWLLHTLRGLAPRTS
                     FWREAQFDTIRLALIKVAARVTELATRIKVSLPSCYPHQDSLALLCARAAKLPP"
     gene            complement(1716..2663)
                     /locus_tag="VLF19_11110"
     CDS             complement(1716..2663)
                     /locus_tag="VLF19_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HSE93844.1"
                     /translation="MNVHQNARTNPYSRALLVARLQGGWTGAAAAASFGISVRTVRKW
                     VARERAEGPAGLVDRSSRPLRSSGRMGAGWRKMILRLRHCRLTAAEIACRLGLARSTV
                     AAELARLGLGQLAALEPKAPIRRYERRRPGDLVHLDIKKLARFDKPGHRATGSRRGQN
                     SKVGFEYVHVCIDDHSRAAYVEVLDDETGDTCARFLARAIVWFALQGITVRRVMTDNG
                     VGYRSHLFRQAWQALGLRHLLTRPYTPKTNGKAERFIKTMLQDWAYAVPYRSSDSRNR
                     QLPRWLKHYNHYRPHSALGDTTPASRLRPTVNNLPGLHS"
BASE COUNT          447 a          976 c          982 g          460 t
ORIGIN      
        1 gccggcgacc aaagcccctc gggaatcaac caggattgga ttgacgaagc cgaactggcg
       61 gatgctggcg gctacctgct ggacgtgctt gggcgagtgc cgccgcgcat tgcgcgtcgc
      121 tgggactagc ccggatcatt cgtgaggcgg tggcaggcgc gacacaagcc gttgagatcg
      181 tgtatgattc tggtgctgga gcagtctcat cacggtgaac ggagttcgcg cccgccatgg
      241 cggatcatgc cccctttctc ccgggcttgt caccggtcgg cggcaagccc gtgcacgtgg
      301 cgttcgacgg cggccggctg acctcggatg cgggggtgct ggtgctggcg gagatcgagc
      361 ggcggctcgg tttggccgag cgcctggcgc gctgcatcga ggacccgcga acgcccgagc
      421 gggtgcgcca tggcttggcc gagatgatcc gctttcgcgc gctcatgatc gccgcgggct
      481 atgccgacgc caacgactgc gacgccctgc gcgccgatcc ggcgttcaag ctggcggtcg
      541 gccggctgcc cgagacgggg gccgatctgt gctcccagcc gacgatgagc cggctcgaga
      601 acctgcccgg cgccaccgcg ctcaagcgca tgatggccgc gatggtcgag ctgttctgca
      661 acagctttga tgaggtcccg cgccggatcg tgctcgacat cgacgacacc gaggaccgag
      721 ttcacggccg ccagcagctg gccttgttcc atgcccacta cgacagtcgc tgctttctgc
      781 cgatccacgt ttatgaggcc cgcagcggca agcccgtcgc ggtgatcctg cgtccgggca
      841 agacgccgga cggcgccgag gtggcgctgg tgctgcgcca cgtggtgcgc gccatccgta
      901 gccattggcc cgccgtcgac atcctgatcc ggggcgacag ccattacggc cgccccgaag
      961 ccatgagctg gctggagcgc aaccgcgtcg gctacatctt cggcctggcc ggcaaccggg
     1021 tcctgctcgc cagggtcggc cacgtcgccg agggcgccgc cctggaccgc gttgccggcg
     1081 gcgccgacaa gatccgccgc tacgccgagt tccactacgg cgccaagact tggcccgccg
     1141 agcggcaggt catcgctcga gtcgaggcct cggcccaggg cagcgacagc cgcttcgtcg
     1201 tcaccaacct caagggcacg ccgcgctggc tgtacgaggc agtctactgt gcccgcggcc
     1261 aggccgagaa tttgatcaag gcgcacaaac tgcacctcgc ctcggatcgc acgtcctgca
     1321 gcaaggccac cgccaaccag ttccggctgc tcgtccacac cgccgcctac tggctcctgc
     1381 ataccttgcg cggcctggca cccaggacct cgttctggcg cgaagcccag ttcgatacca
     1441 tccgcctcgc cctgatcaag gtcgcggcgc gggtcaccga gctggccacg cggatcaagg
     1501 tctccctgcc atcctgctat ccccaccagg acagcctcgc gctcctctgc gcccgcgccg
     1561 ccaagctgcc gccctgagcg gcggggctgc gtgccccgcc cgagcccgtc cccgcaacct
     1621 ccaaccccgt ccgatcgcgt cttcagcgcc accgaccagc acgacccaaa acgggcaaat
     1681 gccggccaaa acaacaaccc gatgaatgat tcaggctagc tgtgtaatcc agggaggttg
     1741 ttcaccgttg gcctgagcct tgaggcgggt gttgtatcgc caagtgcgct atgcggtcgg
     1801 tagtgattgt agtgcttcag ccaccttggc agctggcggt tgcggctgtc ggatgaccga
     1861 taggggacgg cataggccca gtcctggagc atggtcttga tgaaccgttc cgccttgccg
     1921 ttggtcttgg gcgtgtaggg gcgggtcagg aggtggcgca ggccgagggc ctgccaagcc
     1981 tggcggaaga ggtggctgcg atagccgacg ccgttgtcgg tcatgacccg gcgcacggtg
     2041 atgccctgca gggcgaacca gacgatggcc cgagccagga agcgcgcgca ggtatcgccg
     2101 gtctcgtcgt cgagaacctc gacataggcg gccctggaat ggtcgtcgat gcagacatgg
     2161 acgtattcga agccgacctt gctgttctgg ccgcggcggc tgccggtggc gcggtgcccg
     2221 ggcttgtcga agcgggcgag cttcttgatg tcgagatgga cgagatcgcc cggccgccgc
     2281 cgctcgtagc gccggatcgg cgccttgggc tcgagcgccg cgagttggcc gaggccgagg
     2341 cgcgccagct cggctgcgac cgtggagcgg gcgagcccga ggcggcaggc gatctcggcg
     2401 gcggtcagcc ggcagtgccg gagccggagg atcatcttgc gccagcccgc gcccatccga
     2461 cccgacgagc gcagtggccg tgaggagcgg tcgaccaggc cggccggtcc ttccgcacgc
     2521 tcgcgggcga cccatttgcg caccgtccgc acactgatgc caaagctcgc ggccgccgcc
     2581 gcgccggtcc agccgccttg gaggcgggcg accagaaggg ctcgactgta cgggttggtc
     2641 cgcgcattct gatggacgtt catccgggtt cccctgagca gtgccggcct cgcaatccgc
     2701 actctcgggc ctcggacccg ggtgaacaac ctccctggat tacacagcta gtgaaaggcg
     2761 ccgggaggat agcacggtcc taggaccata ttccacgaca acgttagccc ggcgtaggcg
     2821 gtcctcaggt agaccggcgg atggtggaag aggtgccgcg caggg
//
LOCUS       DATIAZ010000320         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_223386, whole genome shotgun sequence.
ACCESSION   DATIAZ010000320 DATIAZ010000000
VERSION     DATIAZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223386"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..1176
                     /gene="tolQ"
                     /locus_tag="VLF19_11115"
     CDS             454..1176
                     /gene="tolQ"
                     /locus_tag="VLF19_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HSE93845.1"
                     /translation="MPPTDALHSGVLDLILNAGPVGKAVLIILLAFSVVSWALIVEKW
                     WQFRRIRRQTLSFLKLWRESRRGATVYAALKKYRESPLAQLYGAAYQEVPAGLDQGLE
                     EMDEGSGAERVEAAQRALRRAATHEVARLERYLPFLATTASATPFIGLFGTVWGVMAA
                     FHGIGQQGSASLAVVAPGISEALIATAFGLGAAIPAVIGYNYFVSRVKHWAAEMDGFA
                     LDLLNVIARPAPKVARSAKDAL"
     gene            1181..1609
                     /locus_tag="VLF19_11120"
     CDS             1181..1609
                     /locus_tag="VLF19_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSE93846.1"
                     /translation="MAFKLDDGDGGRRVGTTLAEINIIPLVDVVLVLLLIFMLTAPMM
                     YRGIDVNLPRAASRPSAVEERMVLTVTKEQSLFLNDRRVTPAGLEQALRDAFKSRTDK
                     TLYLKADAGLSYGAVVETMDRVRRAGIERLGMVTEPVRER"
     gene            1626..2534
                     /locus_tag="VLF19_11125"
     CDS             1626..2534
                     /locus_tag="VLF19_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSE93847.1"
                     /translation="MTVRLRPRSRLGPALRRVHVPFGPLALSAVAHAALAAVLVAAAL
                     LWPAQKTKTYIVNLVPAVPAVGAPHGRPKPADLPPRAATPPPRPETPPARELPPVPRE
                     LPPAPRELPPAPRELPPTRNRELPPPSRESAALPDRPLPPRPAALPRAGDKELPTVPR
                     AASTQRPTPSLAPAKEVAVPSPPPPPAPALGQAAGSAQGAGAVTLNVSDFPYAWYIQA
                     IHRKIQERWEGRAIQGHQPEVIFEIARDGQLKRVAIGKSSGNPAYDQIAMRAIAEANP
                     FPRLPDGFDKSVLTIGLQFVYDPTTR"
     gene            2539..>2742
                     /locus_tag="VLF19_11130"
     CDS             2539..>2742
                     /locus_tag="VLF19_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93848.1"
                     /translation="MRRTFATLVALPLLVVLAMPSAAPPQSSQGVDVWMNIQGAGAKK
                     LNIAIPEFAVVAGPDTTGLAKQLA"
BASE COUNT          396 a         1040 c          939 g          367 t
ORIGIN      
        1 gcgacgagtc ggcggctgcg gttctgggtc gacggcagcg cgcacgtatc gagggtgggt
       61 ccgaccggtc gccgagagag cccggccggc gaggacgacg acggccacct gagcggccgc
      121 cgcgcaacgc ctccgggacc aacagcgcgt cgggcagccc ccggtccacg gcgatcgcgc
      181 gcacgacgat gacgggtccg ggaggtgtcc ggcctcgcgc cgatcaagaa ccgggcgccc
      241 gtgggaacgc ctcgacagcg tgccgcccca cggtcggcct cggccatggg ggtagcgccg
      301 ccaggagcag gaccgtcggc cgaacgcaca ccgtagccgt cgagcagggc cggcgcgacg
      361 gaagtcaacg aaacgaatac actttccgcc aaaataactt tgacactccg cgcaacgggt
      421 gggtagtatc gagggaaacg tcaaggagac gccgtgccgc ccactgacgc gcttcacagc
      481 ggggttctcg atctcatcct caatgccggc cccgtcggca aggccgttct gatcatcctg
      541 ctggccttct cggtcgtgag ctgggccctc atcgtcgaga agtggtggca gttccgccgc
      601 atccgccggc agacgctgtc cttcctcaag ctctggcgcg agtcgcggcg aggcgcgacg
      661 gtctacgccg ccttgaagaa gtatcgggag agccccctgg cgcagctcta cggcgccgcg
      721 taccaggagg tgccggcagg gctcgaccag gggctcgagg agatggacga ggggtcgggc
      781 gccgagcgcg tcgaggcggc ccagcgcgcc ctccgtcgcg cggccacgca cgaggtggcg
      841 cggctcgagc gctacctgcc gttcctggcg acgaccgcga gcgcgacgcc gttcatcgga
      901 ctgttcggca ccgtctgggg cgtcatggcc gccttccacg gcatcggcca gcagggctcc
      961 gcctcgctcg ccgtcgtggc accgggcatc tcggaagccc tcatcgccac cgcgttcggt
     1021 ctcggcgcgg ccatccccgc cgtcatcggc tacaactact tcgtcagccg cgtgaagcac
     1081 tgggcggccg agatggacgg cttcgcgctc gacctcctca acgtgatcgc ccgccccgcc
     1141 cccaaggtgg cccgctcggc caaggacgcg ctctaggcgg atggcgttca agctcgacga
     1201 cggcgacggc ggccggcgcg tcggcaccac gctcgcggag atcaacatca tcccgctggt
     1261 ggacgtcgtg ctcgtgctgc tgctgatctt catgctgacg gcgccgatga tgtaccgcgg
     1321 catcgacgtg aacctgcccc gcgccgcctc ccggccctcg gccgtcgagg agcggatggt
     1381 gctgacggtg acgaaggagc agagcctgtt tctcaacgac cgccgggtca cgccggcggg
     1441 cctcgagcag gcgctgcgcg acgcgttcaa gagccgcacc gacaagacgc tctacctcaa
     1501 ggcggacgcc gggctctcgt acggcgcggt ggtggaaacc atggatcgcg tgcggcgcgc
     1561 cgggatcgag cggctcggca tggtgacgga gcccgtccgc gagcgttgag atgtcgacgg
     1621 cggccatgac ggtccggctg cgtccccgat cccggctcgg ccctgcgctc cgccgcgtcc
     1681 acgtgccgtt cggcccgctg gcgctgtcgg cggtggctca tgcggcgctc gccgccgtcc
     1741 tcgtcgcggc cgcgctgctc tggccggcgc agaagacgaa gacgtatatc gtgaacctcg
     1801 tccctgccgt cccggccgtc ggtgcaccgc acggccgccc caagcccgcc gatctgccgc
     1861 cgcgggccgc gacgccgccg ccgcgcccgg agacgccccc ggcgcgcgag ctgccgccgg
     1921 tcccgcgcga gctgccgccg gccccccgcg agctgccgcc ggccccgcgc gagctcccgc
     1981 cgacgcgcaa tcgcgagctg ccgccgccct cgcgcgagtc ggcggcgctt cccgaccggc
     2041 ccctcccgcc gcgcccggcc gcgctgccgc gtgcgggcga caaggagctg ccgactgtgc
     2101 cacgggctgc ctccacccag cggccgacgc cctcactcgc accggcgaaa gaggtcgcgg
     2161 tgccctcgcc gcccccgccg cccgcgccag cgctcgggca ggccgccggc tcggcgcagg
     2221 gcgccggcgc cgtcacgctc aacgtctccg acttcccgta tgcctggtac atccaggcga
     2281 tccaccggaa gatccaggag cgctgggagg gccgcgcgat ccaggggcac cagcccgagg
     2341 tcatcttcga gatcgcgcgt gacggacagc tcaagcgggt cgccatcggc aagagctcag
     2401 gcaacccggc gtacgaccag atcgccatgc gcgccatcgc cgaggccaac ccgttcccgc
     2461 ggctgcccga cgggttcgac aagtccgtgc taaccatcgg cctgcaattc gtctacgacc
     2521 ccaccacgag gtaagccagt gagacgtaca ttcgctacgc tcgtcgccct cccgctgctg
     2581 gtcgtcctcg cgatgccctc cgccgcgccg cctcagagtt cgcagggcgt ggacgtctgg
     2641 atgaacatcc agggcgcggg ggccaagaag ctcaacatcg cgatccccga gttcgccgtc
     2701 gtcgcgggcc cggacacgac cggactcgcc aagcagctcg cc
//
LOCUS       DATIAZ010000321         8129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_223473, whole genome shotgun sequence.
ACCESSION   DATIAZ010000321 DATIAZ010000000
VERSION     DATIAZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223473"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..745)
                     /locus_tag="VLF19_11135"
     CDS             complement(230..745)
                     /locus_tag="VLF19_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HSE93849.1"
                     /translation="MSDRAAAWTIGVVSAVLVLGVGVLLLGPRAGASELDVSALPALN
                     ATLNGVSAVLLAAGYGFIRRRRVAAHVACMLAAFGVSALFLVSYVVYHWHAGSRPFTG
                     QGWIRPVYFALLVTHVVLAAAIVPLALTTIWRGASAQFGRHVRIARFTLPVWLYVSVT
                     GVLIFVMLYRL"
     gene            complement(742..2892)
                     /locus_tag="VLF19_11140"
     CDS             complement(742..2892)
                     /locus_tag="VLF19_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSE93850.1"
                     /translation="MSTASSDRAARALAAFPRERQWLLPALHTVQHALGWLPPAALAE
                     VAAHLRVPASEVHGVATGYPELRLAPRGRHHVRVCTGVSCALAGGGRRLLDAIGRERG
                     VSPGETGAERALTLEGTDCFFQCAVAPLIEVDGELHGRVTPDAAGRLDDWFSFRRRHG
                     SPTPEGRAPVSSAPLPAPTAQEALARLAADARRAPRPRLRLLVQMGTCGRAVGGADLL
                     RAVRDGARERQLDAEVVEGACAGACWAAPSVEIAGEGMPRALVERLEPGRVPSLLTAL
                     AARSGDLPALGLTGVAWAATPWRGLVPVGAHPFWRGQERVLLARAGAIDPESLADALR
                     AGAYAALARALDGRPEDVIEAVKAAGLQGRGGAFFPAALKWEACRRAPGGTKFVVVNG
                     EEGEPGIFKDRHLMEGDPHQVLEGVLLAAWAAGAGRAILYIHGEAALAAERLAWAVAE
                     ARAAGIVGERVLGRGVACEVEIRRGAGGFVLGEETALLESIEGRRAQPRTRPPFPVES
                     GLWGRPTVINNVETLAAVPSIVAHGSAWFAALGTAAASGTKVFGLSGPIGRPGVVETR
                     NGGTLRALLEDVGGGAADGRPLKGAVVGGPSGSVVPAGLFDVPMEPRGRVSPGTGGVV
                     GVPEDASVRDIVRTLVAFNAQESCGKCTPCREGLPRLVALLDALGGDPDAEARGRALA
                     ETIQLASLCGLGQAAPLAFVRGLEAFGPDLKARR"
     gene            complement(2873..4543)
                     /locus_tag="VLF19_11145"
     CDS             complement(2873..4543)
                     /locus_tag="VLF19_11145"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HSE93851.1"
                     /translation="MSPGPRPISEIASELGIQPDELEPYGRYKAKVSLGVLPRLAGRR
                     RGVYVDVTCITPTPLGEGKTVTTIGLAMALNRIGCRAVAAIRQPSAGPVFGVKGGGAG
                     GGRAQVVPLDEVNLHLTGDIHAVALAHNLLAAMIDNALMHGKGPALDPHTITWPRVVD
                     VNDRALRRVVVGLGGRENGTPRETGFEIAVASEAMAILGLTGGYADLRRRLGRVVVGL
                     ARDGAPVTADDLKGAGAMAAVMRETVKPNLLQTSEGTPAFVHAGPFANIAHGNSSILA
                     DQIGLGLTDCVVTESGFGADMGLEKFANIKCRASGLAPDVIVVVCTVRALKMHSGRYR
                     VAAGRPLDPAMLREDLVALGEGCANLDKQVENARQYGVPVVVAINAFETDTGAEHALI
                     RDRAQAAGAIGAWRSDVFSLGGAGAEELAAAVLKAAEGPRQFRFLYELDRPVKEKIET
                     IATRLYGADGVDVSPAAERAIARWSELGYGGLPVCMAKTHLSLSHDPRLLGRPRGFRL
                     PVREVRLSAGAGFLYVLCGDMMTMPGLPSEPNAWRIDIDDQGNVHGLF"
     gene            complement(4550..7342)
                     /gene="fdhF"
                     /locus_tag="VLF19_11150"
     CDS             complement(4550..7342)
                     /gene="fdhF"
                     /locus_tag="VLF19_11150"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HSE93852.1"
                     /translation="MRLTIDDRAVEVAPGATVLDAVNALGIPLPQLCKDPDRPPLGAC
                     RTCLVHVDGQRGTPAACHLPVREGMIVSTVHPDVVRVRASVLDLTRSMLTPGPDRRGF
                     GQLGEVCARHGLERPSSAALHHFEVDASKSFFVLDREACILCGRCTDACDDVQQIGAI
                     AMLGRGHRMKVGVAGDAPMAASVCTSCGQCVATCPTGALRPKDEPAPVTRRVETTCPY
                     CGVGCGIVARVTAAGRLAVMADDVPANGSSLGMLCVKGRFGTGFVHARDRVTTPLVKR
                     DGRWRETTWDEALDAAAAGLARHHGRFGALASAKATNEDGYLVQKLVRAVMGTNNVDH
                     CTRLCHSPSVEAMLASMGSGATSNSYVDYEEAGCLMIVGADASANHPVIAIRFRRAMS
                     RGARIVVVNPKRIELCHQADLWLAQRPGTDVALFNAMARVILDEGLADLEFVRARTEG
                     FEEWRRSLEPFTLDHAARVTAVPAQDIRQAARWYARPPITRKEGSAPFTTPRAGSCLV
                     WGMGITQHTNGIHNAHALLNLSLVAGQLGFPGSGISPLRGQNNVQGCGDAGCIPTNLP
                     GYQAYDGETLAKFERAWGVRPPETTGLVVTEMVEACLTGGITAMYVVGENPLLSEPDL
                     HHAEKAFGQLGFLVVQDLFMHETAERADVFLPAAAFAEKEGTFTNSERRVQRVRQALE
                     AQGQARPDWWITCELAKRVGRKLGRDLDRQFTYAGAAEVFDEMARLVPFLSGLSHARL
                     DREGGIQWPCPAPDHPGTRFLYAESFPRGKGKFIPAPQTREAAELPDADYPLILNTGR
                     LLYHWHGGTITRRVPGLVELAPRLEVTIHPADAGRAGVVDGEPVRVTSRRGELVGLAR
                     VTDAVRPGAVFVPFVKLDESAANFLTNSASDPMSKIPEYKVCAVRIERASRVEGTGAT
                     HPGGQSGGRRASLDVD"
     gene            7594..7983
                     /locus_tag="VLF19_11155"
     CDS             7594..7983
                     /locus_tag="VLF19_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HSE93853.1"
                     /translation="MRSELQPSSNHRVSGLVDVLDRVLDKGLAIAGDIKISLAEVELL
                     TIRIRLIICSIDKAQEIGLDWWKHDRHLAPGRLGAAHTDDLSQQLRALERKVALLSGE
                     RPLTAAVGHAAGARAPKSRRRRAPDGR"
     gene            7973..>8129
                     /gene="gvpJ"
                     /locus_tag="VLF19_11160"
     CDS             7973..>8129
                     /gene="gvpJ"
                     /locus_tag="VLF19_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HSE93854.1"
                     /translation="MAVERTSGGTSLIDVLDRVLDKGIVIDAWVRVSLVGIDLITVEA
                     RVVVASIE"
BASE COUNT         1136 a         3042 c         2936 g         1015 t
ORIGIN      
        1 tcgagcgtcg tggccctacg tatgcggcga tggcccagcg gcgggagacg cgcctcgagc
       61 ccggcaacgg cgattccgtg tgccgggtgt gcttgcagcc gatccgggcg gcggacaagg
      121 tgcgcggctc ggccgccagt ctcatccacg aagcctgcga ctacacgcgc gcgcgcggtg
      181 gcggcacgaa cgatgggcga gccggggccg ggcccacgtc ctcgaggtct cagagccggt
      241 agagcatcac gaagatcagg acgccggtga ccgacacgta gagccagacc ggcagcgtga
      301 agcgcgcgat cctcacgtgg cggccgaact gggccgaggc gccgcgccag atggtcgtca
      361 gcgcgagggg cacgatcgcc gccgccagca cgacgtgggt gaccagcagc gcgaagtaca
      421 ccggccggat ccagccctgg ccggtgaacg gccgggaccc ggcgtgccag tgatagacga
      481 cgtaggagac gaggaagagc gcggagacgc cgaaggcggc cagcatgcac gccacgtgcg
      541 cggccacgcg gcgccgccgg atgaagccgt aacccgccgc cagcagcacc gcgctcacgc
      601 cgttgagcgt cgcattgagc gccggcagcg ccgagacgtc gagctcgctc gcgcccgcgc
      661 gcgggccgag cagcagcacg cccacgccga ggacgagcac cgcggagacc acgccgatgg
      721 tccacgccgc ggcgcggtcg ctcaccggcg cgccttcagg tccggtccga acgcctcgag
      781 cccccgcacg aacgcgagcg gcgcggcctg acccaggccg cagagcgagg cgagctggat
      841 ggtctccgcc agcgcccgcc cacgcgcctc ggcgtcgggg tcgccgccga gcgcgtcgag
      901 cagcgccacc aggcgcggca ggccttcgcg gcacggcgtg cacttgccac acgactcctg
      961 cgcgttgaac gccacgagcg tgcgcacgat gtcccggacc gaggcgtcct ccggcacgcc
     1021 gaccacgccg cccgttcccg gcgacacgcg cccccgcggc tccatcggca cgtcgaagag
     1081 cccggccggc accacgctgc ccgatggccc gccgaccacc gcgcccttga gcggccggcc
     1141 gtcggccgcg ccaccaccca cgtcctcgag cagcgcgcgc agcgtgccgc cgttacgcgt
     1201 ctccacgacg ccgggtctgc cgatcggccc cgacagcccg aacaccttcg tgccggacgc
     1261 ggcggccgtt ccgagcgcgg cgaaccacgc gctcccgtgg gccacgatcg acggcaccgc
     1321 ggcgagggtc tccacgttgt tgatgacggt cgggcgtccc cacagacccg actcgaccgg
     1381 aaacggcggg cgcgtgcgcg gctgcgcgcg gcgaccctcg atcgattcga gcagcgccgt
     1441 ctcctcgccc agcacgaagc caccggcccc gcgccggatc tcgacctcgc acgcgacgcc
     1501 gcgtccgagc acgcgctcgc cgacgatccc ggccgcgcgc gcctcggcca cggcccaggc
     1561 caggcgctcg gcggcgagcg cggcctcgcc gtgaatgtag aggatggcgc gcccggcgcc
     1621 ggcggcccac gcggcgagca gcacaccttc cagcacctga tgcggatcgc cctccatgag
     1681 gtggcggtcc ttgaagatcc cgggctcacc ctcctcgccg ttcacgacga cgaacttcgt
     1741 cccgcccggc gcccgccggc acgcctccca cttgagcgcc gccgggaaga aggcgccgcc
     1801 tcgcccctga aggccggcgg ccttgaccgc ctcgatgacg tcctccggac ggccatccag
     1861 ggcgcgcgcg agcgcggcgt aggcgccggc tctcagcgcg tcggcgaggc tctcggggtc
     1921 gatcgcgccg gcgcgcgcca gcagcacgcg ctcctgcccg cgccagaacg ggtgagcgcc
     1981 caccggcacg agtccgcgcc atggcgtggc cgcccaggcg acgccggtca gccccagggc
     2041 cggcaggtcg ccggaccgcg ccgcgagcgc ggtgaggagc gagggtacgc gaccgggctc
     2101 gaggcgctcg acgagcgccc gcggcatccc ctcacccgcg atctccaccg acggcgcggc
     2161 ccagcaggcg ccggcgcagg cgccctcgac gacctcggcg tcgagctggc gctcgcgcgc
     2221 gccgtcgcgg accgcgcgca gcaggtcggc gccgccgacg gcgcgaccgc acgtgcccat
     2281 ctgcacgagg agcctcagcc tgggccgcgg cgcgcggcgg gcatcggcgg cgagccgggc
     2341 gagcgcctct tgcgcggtgg gcgcgggcag tggcgcgctc gacaccggcg cccgcccctc
     2401 gggcgtgggg ctgccgtgac ggcgccggaa ggagaaccag tcgtcgaggc gccccgcggc
     2461 gtcgggcgtc acgcgcccgt gcagctcgcc gtccacctcg atcagcggcg ccaccgcgca
     2521 ctggaagaag cagtcggtcc cctccagcgt cagcgcgcgc tcggcgccgg tctctcccgg
     2581 cgacacgccg cgctcccgcc cgatcgcgtc cagcaggcga cggccgccgc cggcgagcgc
     2641 gcacgagacg ccggtgcaca cgcgcacgtg gtgacggccg cgcggcgcca gccggagctc
     2701 cggatagccg gtcgccaccc cgtgcacctc gctggcgggc acccgcaggt gcgccgcgac
     2761 ctccgcgagc gcggccggcg gcagccagcc cagcgcgtgc tgcaccgtgt ggagcgcggg
     2821 gaggagccac tgccgctcgc gcgggaaggc cgcgagcgcc cgcgcggcgc ggtcagaaga
     2881 ggccgtggac attcccctgg tcgtcgatgt cgatgcgcca ggcgttgggc tccgacggca
     2941 gcccgggcat cgtcatcatg tcgccacaga gaacgtagag gaagcccgcc cccgccgaga
     3001 ggcgcacctc gcgcacgggc agccggaagc cgcggggccg gccgagcagc ctggggtcgt
     3061 gcgagagcga caggtgcgtc ttggccatgc acaccggcag gccgccgtag ccgagctcgc
     3121 tccagcgcgc gatcgcgcgc tcggcggccg gcgagacgtc cacgccgtcg gcgccgtaga
     3181 gccgcgtcgc gatggtctcg atcttctcct tcaccgggcg gtcgagctcg tagaggaagc
     3241 ggaactgccg cggcccttcg gccgccttga gcacggccgc ggccagctcc tcggcgccgg
     3301 caccgccgag gctgaagacg tcgctgcgcc aggcgccgat ggcgcccgcg gcctgcgcgc
     3361 ggtcgcggat cagcgcgtgc tcggccccgg tgtcggtctc gaaggcgttg atggccacca
     3421 cgacgggcac gccgtactgc cgcgcgttct ccacctgctt gtcgaggttg gcgcagccct
     3481 cgccgagcgc gaccaggtct tcgcgcagca tcgccgggtc gagcgggcgg cccgccgcca
     3541 cccggtaccg gccgctgtgc atcttgaggg cgcgcacggt gcacacgacg acgatcacgt
     3601 cgggggcgag gcccgaagcg cgacacttga tgttcgcgaa cttctcgagt cccatgtcgg
     3661 cgccgaagcc gctctcggtg accacgcagt cggtcaggcc caggccgatc tggtcggcca
     3721 ggatcgagga gttgccgtgg gcgatgttgg cgaagggccc ggcgtggacg aaggccggcg
     3781 tgccctcgct cgtctgcagg aggttcggct tcacggtctc ccgcatgacc gccgccatgg
     3841 cgccggcgcc tttcagatcg tcggcggtga cgggcgcgcc gtcgcgcgcg aggccgacca
     3901 cgacgcgtcc caggcgtcgc cggaggtcgg cgtagccgcc cgtcagcccc aggatcgcca
     3961 tcgcctcgga ggcgacggcg atctcgaagc ccgtctcccg cggcgttccg ttctcccggc
     4021 ccccgagccc gacgacgacg cgccggagcg cgcggtcatt gacgtccacg acgcgcggcc
     4081 acgtgatggt gtgcgggtcg agggccgggc ccttgccgtg catcagcgcg ttgtcgatca
     4141 tcgccgcgag caggttgtgg gcgagcgcca ccgcgtggat gtccccggtg aggtggaggt
     4201 tgacctcgtc cagcgggacc acctgggcgc gtccgccgcc ggcgccgccg cccttgacgc
     4261 cgaagaccgg ccccgccgag ggctgccgga tggcggccac cgcgcggcag ccgatgcggt
     4321 tgagcgccat cgccagcccg atcgtggtga cggtcttgcc ctcgccgagc ggcgtcggcg
     4381 tgatgcaggt cacgtccacg tacacgccgc ggcggcgtcc ggccaggcgc ggcaacacgc
     4441 cgagcgagac cttggccttg taccggccat agggctccag ctcatcgggc tggatgccga
     4501 gttcgctcgc aatctcggag atcgggcggg gtccggggga caagcgcgat cagtcgacgt
     4561 cgagggaggc gcgacgtccc cccgactgcc cccccggatg cgttgcgccc gtcccttcca
     4621 cccggctcgc gcgctcgatc cggacggcgc agaccttgta ctcgggaatc ttcgacatcg
     4681 gatcggaggc ggagttcgtc aggaagttgg cggccgactc gtcgagcttc acgaacggca
     4741 cgaagaccgc gcccgggcgc acggcgtcgg tcacgcgcgc cagccccacc agctcgccgc
     4801 gccgcgacgt cacccgcacc ggctcgccgt cgacgacgcc ggcacgcccg gcgtcggccg
     4861 gatggatcgt cacctccagc cgcggcgcca gctccacgag tcccggcacc cggcgcgtga
     4921 tcgtgccgcc atgccagtgg tagagcaagc gccccgtgtt gaggatcagc gggtagtcgg
     4981 cgtccggcag ctcggcggcc tcccgcgtct gcggcgccgg gatgaacttc cccttcccgc
     5041 gggggaagga ctcggcatac aggaaccgcg tgccgggatg gtcgggcgcc gggcacggcc
     5101 actggatccc gccctcgcgg tccagccgcg cgtgcgagag gcccgagagg aacggcacca
     5161 ggcgcgccat ctcgtcgaac acctccgcag cgccggcgta cgtgaactgc cggtcgaggt
     5221 cgcgaccgag cttccgtccc acgcgcttgg cgagctcgca cgtgatccac cagtccggcc
     5281 gcgcctgccc ctgggcttcc agcgcctggc ggacccgctg cacgcgccgc tccgagttcg
     5341 tgaatgtgcc ctccttctcg gcgaaggccg cggcgggcag gaacacgtcg gcccgttccg
     5401 ccgtctcgtg catgaagagg tcctgcacga cgaggaagcc gagctggccg aaggccttct
     5461 ccgcgtggtg gaggtcgggc tccgagagca gcgggttctc acccacgacg tacatcgcgg
     5521 tgatgccgcc ggtgaggcag gcctccacca tctccgtcac cacgaggccg gtggtctccg
     5581 gcggtcgcac gccccatgca cgctcgaact tcgccagggt ctcgccgtcg tacgcctggt
     5641 aacccggcag gttggtcggg atgcagccgg cgtcgccgca gccctggacg ttgttctggc
     5701 ctcgcagcgg cgagatgccg gagcccggaa agccgagctg gccggccacg agcgacaggt
     5761 tcagaagcgc gtgggcgttg tggatgccgt tggtgtgctg cgtgattccc ataccccaca
     5821 cgagacagga gccggcccgg ggagtcgtga agggggcgga gccctctttc ctggtgatcg
     5881 gggggcgggc gtaccagcgg gcggcctgcc ggatgtcttg cgccggcacg gccgtgacgc
     5941 gggcggcgtg atcgagcgtg aagggctcga gtgaccggcg ccactcctcg aacccctccg
     6001 tgcgcgcgcg gacgaactcc aggtccgcca ggccctcgtc gaggatcacc cgcgccatgg
     6061 cattgaagag cgcgacgtcc gtgccgggcc gctgcgcgag ccagaggtcg gcctggtggc
     6121 acagctcgat gcgcttgggg ttcacgacca cgatccgggc gccccggctc atcgcccgcc
     6181 ggaagcggat cgcgatgacc gggtggttcg ccgaggcgtc ggcgcccacg atcatcaggc
     6241 agccggcctc ctcgtagtcc acgtaggagt tcgacgtcgc gcccgagccc atggaagcga
     6301 gcatggcctc cacggacgga gagtggcaga gccgcgtgca gtggtcgacg ttgttcgtgc
     6361 ccatgaccgc ccgcacgagc ttctgcacga ggtagccgtc ctcgttcgtg gccttggcgc
     6421 tggccagtgc gccgaagcgc ccgtggtggc gggccaggcc cgcggccgcg gcgtcgagag
     6481 cctcgtccca ggtggtctcg cgccaccgcc cgtcccgttt cacgagggga gtcgtcacgc
     6541 ggtcgcgcgc gtggacgaag ccggtgccga accgcccctt cacgcacagc atgccgaggc
     6601 tcgagccgtt cgcgggcacg tcgtcggcca tgaccgccag ccgccccgcc gcggtcaccc
     6661 gggcgacgat cccgcacccc accccgcagt aggggcaggt cgtctcgacg cggcgggtga
     6721 ccggcgcggg ctcgtccttc ggccgcagcg cgccggtggg acaggtggcg acgcactgac
     6781 cgcacgacgt gcagaccgag gccgccatgg gcgcgtcgcc ggcgacgccg accttcatgc
     6841 ggtggcctcg ccccagcatc gcgatggcgc cgatctgctg cacgtcgtcg caggcgtccg
     6901 tgcagcggcc gcagaggatg caggcctcgc ggtcgaggac gaagaaggac ttgctcgcgt
     6961 ccacctcgaa gtgatggagc gccgccgagg acggccgctc gagcccgtga cgcgcgcaga
     7021 cctcgcccag ctgcccgaac ccgcggcggt cgggtcccgg cgtcagcatg gatcgcgtga
     7081 ggtccagcac cgaggcccgc acccggacga cgtcggggtg caccgtgctc acgatcatgc
     7141 cctcgcggac cggcaggtga cacgcggccg gcgtgccccg ctggccgtcc acgtgcacga
     7201 ggcaggtccg gcaggcgccc agtggcgggc gatccgggtc cttgcagagc tgcggcagag
     7261 ggatgccgag cgcgttcacc gcatcgagga ctgtggcgcc gggtgccacc tccacggcac
     7321 ggtcgtcgat cgtcagcctc atcggcgatc attgtacgtc agcgaggtac gcgagacggc
     7381 ggctccggcg gctaccgctc gggcggcccg ccgccggaac ctcgcatccc ggagcgcggg
     7441 cagcacctcg cgctcgcaga gcgaggagcc ggtggccagt tccccgccag ggggctctag
     7501 gcggcatggg aaaaagattc cccaccttcg ctcgcctggc gaaatgccgc tttctagaga
     7561 tagggggcgc gtctatactt tccgtgcgcc cccatgagat ccgagcttca gcccagctcc
     7621 aatcaccgcg tcagcggact cgtcgacgtg ctggaccggg tgctcgacaa ggggctggcc
     7681 atcgccggcg acatcaagat ctcgctggcc gaggtcgagc tgctcacgat ccgcatccgg
     7741 ttgatcatct gctcgatcga caaggcgcag gagatcgggc tggactggtg gaaacacgac
     7801 cgccacctcg cgccgggtcg gctcggggcg gcgcacaccg atgacctctc ccagcagctg
     7861 cgcgcgctcg agcgcaaggt cgcgctcttg tccggcgagc gcccgctgac cgccgccgtc
     7921 ggccacgcgg ccggagcgcg cgcaccgaag agccgtcgta ggagggcacc cgatggccgt
     7981 tgaacgaaca tccggcggca cgagcctgat cgacgtcctc gatcgggtcc tggacaaggg
     8041 catcgtcatc gatgcgtggg tccgcgtctc gctcgtcggc atcgacctga tcacggtcga
     8101 ggcccgcgtc gtcgtcgcat cgatcgaga
//
LOCUS       DATIAZ010000322         4042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_94526, whole genome shotgun sequence.
ACCESSION   DATIAZ010000322 DATIAZ010000000
VERSION     DATIAZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94526"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..668
                     /locus_tag="VLF19_11165"
     CDS             90..668
                     /locus_tag="VLF19_11165"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HSE93855.1"
                     /translation="MVIAAAKHLMLIKGYHATSLDEVLTESGVGKGSFYHFFRSKEDL
                     GHAILDQIIASFIAKTLDPCFSDPDGRPLAQIRCFLDRVVETQRAGNCVGGCPLGNLA
                     SELSDVHEGFRARLVDVFSAWRARLTEALREAQRRGDVGSECRPDAVAHFLVASLEGA
                     ILMAKVSKDIAVMEQCVDEMRRYLAIYERGAR"
     gene            665..1276
                     /locus_tag="VLF19_11170"
     CDS             665..1276
                     /locus_tag="VLF19_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSE93856.1"
                     /translation="MSVAPGPRVDPDAPLVEGLRREDAEAMEQLVERYGDRVYRLAVR
                     ITGSNEDAEEVVQDALWTAARKIQTFKGDSAFGSWLYRVTANTAYMKLRSRRSRQREI
                     ALDDVLPALDDGGLHFEPMDDWSLRVDERALQGELRQVLERSIGELPADYRTALVMHD
                     IEGLSNPDIAAALSISLPAVKSRVHRSRLFLRKRLSEYLGPKT"
     gene            1507..1818
                     /gene="rplU"
                     /locus_tag="VLF19_11175"
     CDS             1507..1818
                     /gene="rplU"
                     /locus_tag="VLF19_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSE93857.1"
                     /translation="MEAVIATGGKQYRMTPGQVIAVEKLPADAGARVQFGDVLLVSRD
                     GEVVAEPARLKSAKVSGEVVGHARAPKVSVVKFKRRKNYRRNRGHRQPATRVRITTIE
                     V"
     gene            1826..2083
                     /gene="rpmA"
                     /locus_tag="VLF19_11180"
     CDS             1826..2083
                     /gene="rpmA"
                     /locus_tag="VLF19_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSE93858.1"
                     /translation="MAHKKGVGSSRNGRDSNPQMLGVKRFAGQFVTGGSILVRQRGTR
                     FKPGTNVGRGSDDTLFAKIDGVVRFERRGDSRLVSIVAAPA"
     gene            2117..3157
                     /gene="obgE"
                     /locus_tag="VLF19_11185"
     CDS             2117..3157
                     /gene="obgE"
                     /locus_tag="VLF19_11185"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSE93859.1"
                     /translation="MFVDEIEIHVKGGDGGAGCVSFRREKYVPHGGPDGGDGGDGGSV
                     WLQADPALSTLLDYHYKRHYEAERGVHGKGANRHGAAGRDLVLRVPVGTVVTDRDRGE
                     LVADLATPGQRVLGQRGARGGRGNARFASSTNRAPRQADPGTYGEGRWLRLELKLLAD
                     VGVIGFPNAGKSTLVSRLSAATPKIADYPFTTLAPSLGIVRVDPERSFVIADLPGLIP
                     GAAQGKGLGHQFLRHTERTRLLLHVLDLDPATGRDPVEDVAVINAELAAYSETLARRP
                     QIVVANKVDLVAAGGAWPEGAARRAAVERLCAREGLPCVAISAATGAGLDDLVRHVAA
                     ALEETGWMRAAS"
     gene            3136..>4042
                     /gene="proB"
                     /locus_tag="VLF19_11190"
     CDS             3136..>4042
                     /gene="proB"
                     /locus_tag="VLF19_11190"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HSE93860.1"
                     /translation="MDARGELTGSRRPPLGGCRRLVVKVGSGLVTAPGAGVEPDRITA
                     VADEVVAVRPGREIVLVTSGAIATGMGRLALAERPRSIPEKQAAAAVGQSALMWQYEV
                     AFKRHGVPVGQVLLTAQDIADRSRYLNARNTLLALLGFGVVPIVNENDTVAVEEIKVG
                     DNDNLSALVASLIDADLLVLLTDVDGLYTGDPAVDPSARKLDLVEAVTDEIERLVWDR
                     ADRVSVGGMATKLEAAQKAAASGIAMVVADGRQRGVLTRLLAGDHVGTYFAPKADRLT
                     ARKRWIAFAVPPQGRLTVDAGAVRAL"
BASE COUNT          580 a         1441 c         1449 g          572 t
ORIGIN      
        1 ctgcctatcc tctgtaccga ctggtcggta caatgacgcc aacaacgacg gccggcacac
       61 gctcgagaga gggcaagcaa acccgcgagg tggtcatcgc ggccgccaag cacctcatgc
      121 tgatcaaggg ctatcacgcg acctccctcg acgaggtgtt gaccgagagc ggtgtcggca
      181 agggcagctt ctaccacttc ttccggagca aggaagacct cgggcacgcg attctcgacc
      241 agatcatcgc gtcgttcatc gcgaagacgc tcgatccatg cttctccgac cccgatggac
      301 gccccctggc gcagatccgg tgcttcctgg accgcgtcgt ggagacgcag cgcgccggca
      361 actgcgtggg cggctgcccg ctcgggaacc tcgcctccga gctgtcggac gtgcacgagg
      421 ggttccgcgc gcgcctggtc gacgtcttct ccgcgtggcg ggcgcgcctg acggaggcgc
      481 ttcgcgaggc gcaacggcgc ggtgacgtgg ggtcggagtg tcgcccggac gcggtggcgc
      541 acttcctggt ggcgtccctc gagggcgcca tcctcatggc caaggtgagc aaggacatcg
      601 cggtgatgga gcagtgcgtg gacgagatgc gacggtacct ggcgatctac gagcgagggg
      661 cacggtgagc gtcgcccccg gtccccgcgt cgatcccgac gccccgctcg tcgaggggct
      721 ccgccgcgag gacgccgagg cgatggagca gctggtcgag cgctacggcg accgcgtcta
      781 tcggctcgcc gtgcgcatca ccggctccaa cgaagacgcc gaggaagtgg tccaggacgc
      841 gctctggacc gcggcgcgca agatccagac gttcaagggc gactccgcct tcggcagctg
      901 gctctaccgc gtgaccgcga acaccgcgta catgaagctg cggtcgcgcc gcagccgtca
      961 gcgcgagatc gccctggacg acgtgctgcc cgcgctcgac gacggcggtc tgcacttcga
     1021 gcccatggac gactggtcgc tccgggtgga cgagcgtgcc ctgcaaggcg agctgcggca
     1081 ggtgctcgag cgctccatcg gcgagctgcc tgccgactac cggacggcgc tggtgatgca
     1141 cgacatcgag ggcctctcga atccggacat cgccgcagcg ctttccatca gcctgcccgc
     1201 ggtcaagtcg cgcgtccacc gctcgcggct cttcctgcgc aaacgcctgt ccgagtacct
     1261 cggccccaag acgtagcgtt cgccggaccg tcggccgcat cgctccgggc cgcctgccat
     1321 gcgcggctcg gtgaggcggt gcgcagccac gacgggccgg cggaccgcgc atgtttcgag
     1381 ccggatcgag tcgccgcgac aacgagatga cgccagtgtc gcccgccgac ttcttgacgc
     1441 ggcccggcgc tccgtgctct catacttgct tgccgccaat cctcttggag aaaggtctgg
     1501 gacgtcatgg aagcggtcat cgcgacgggg ggcaagcagt atcgcatgac gccggggcag
     1561 gtcatcgccg tcgagaagct gccggcggac gcgggagccc gcgtgcagtt cggcgacgtg
     1621 ctcttggtca gccgggacgg cgaggtcgtc gccgagccgg cgcggctgaa gtcggcgaag
     1681 gtgtccggcg aggtcgtcgg tcacgcgcgc gcgccgaagg tctcggtcgt caagttcaag
     1741 cggcgcaaga actatcggcg gaaccgcggg caccggcagc cggccacgcg cgtccgcatc
     1801 acgaccatcg aggtgtagcg gagccatggc acacaagaaa ggcgtggggt cttcccgcaa
     1861 cggccgcgac tcgaacccac agatgctggg cgtcaagcgg ttcgccgggc agttcgtcac
     1921 cggcggcagc atcctggtcc ggcagcgcgg cacgcgcttc aagcccggca ccaacgtcgg
     1981 ccggggctcc gacgacacgc tgttcgcgaa gatcgacggc gtcgtccgct tcgagcggcg
     2041 gggagactcc cgcctcgtga gcatcgtcgc ggcgccggcc tgaccatcgg cgggcgtccc
     2101 ccggcccgcg gcgcacatgt tcgtcgacga gatcgagatc cacgtcaagg gcggtgacgg
     2161 gggcgccggc tgcgtgagct tccgtcgcga gaagtacgtc cctcacggcg gccccgacgg
     2221 cggcgacggc ggcgacggcg gcagcgtgtg gttgcaggcc gatcccgcgc tttccacgct
     2281 gctcgactac cactacaagc gccactacga ggccgagcgc ggtgtccacg gcaagggggc
     2341 caaccgtcac ggcgcggcgg gtcgcgacct ggtcctgcgc gtccccgtgg gcaccgtcgt
     2401 cacggaccgc gaccgcggtg agctcgtcgc cgatctcgcc acgcccggcc agcgcgtgct
     2461 cggccagcgc ggggcccgcg gtggccgcgg caacgcccgg ttcgcctcgt cgaccaaccg
     2521 cgcgccgcgg caggccgacc ccggcaccta cggcgagggc cgatggctcc gtctcgagct
     2581 gaagcttctg gccgacgtgg gcgtgatcgg cttccccaac gccggaaagt ccacgctggt
     2641 ctcgcgcctg agcgccgcga cgccgaagat cgcggactat ccgttcacca cgctcgcgcc
     2701 ctccctgggt atcgtgcgcg tggaccccga gcgtagcttc gtcatcgccg acctgccggg
     2761 gctcatcccc ggggccgcgc agggcaaggg gctcggacac cagttcctcc gccacaccga
     2821 gcggacgcgc ctgctgctgc acgttctcga tctcgatccg gccacggggc gcgaccccgt
     2881 cgaggacgtc gcggtcatca acgccgagct ggccgcttac tcggagacgc tcgcgcgccg
     2941 gccccagatc gtcgtcgcca acaaggtgga cctggtggcc gccggcggcg cctggccgga
     3001 gggcgccgcg cggcgcgctg ccgtggagcg gttgtgcgcg cgcgagggcc tgccgtgcgt
     3061 cgcgatctct gcggccaccg gcgccggcct ggacgacttg gtgcgtcacg tcgcggcggc
     3121 gctcgaggag accggatgga tgcgcgcggc gagctgaccg gctcccgccg cccgccgctc
     3181 ggcggctgcc gccggctcgt ggtgaaggtc ggcagcgggc tcgtgaccgc cccaggggcc
     3241 ggcgtcgagc ccgaccgcat caccgcggtc gccgacgagg tcgtcgccgt gcggccgggc
     3301 cgcgagatcg tgctcgtcac gtcgggcgcg atcgccaccg gcatgggacg cctcgcgctc
     3361 gccgagcgcc cgcgctcgat ccccgagaag caggcggcgg ccgcggtggg gcagtcggcc
     3421 ctgatgtggc agtacgaggt cgccttcaag cgccacggcg tgcccgtcgg tcaggtgctg
     3481 ctgacggcgc aggacattgc cgatcgctcg cgctacctga acgcgcgcaa cacgctgctc
     3541 gcgctgctcg gcttcggcgt cgtgccgatc gtcaacgaga acgacaccgt cgccgtcgag
     3601 gagatcaagg tcggcgacaa cgacaacctg tcggcgctcg tcgcctcgct catcgatgcc
     3661 gacctgctcg tgttgctgac cgacgtggac ggtctctaca cgggcgaccc cgcggtggac
     3721 ccgtccgccc ggaagctcga cctggtggag gcggtcaccg acgagatcga gcgcctggtg
     3781 tgggaccggg cggaccgcgt gtccgtgggc ggtatggcca ccaagctcga ggcggcgcag
     3841 aaggcggcgg cgtcgggcat cgccatggtc gtcgccgacg gccgccagcg gggcgtgctc
     3901 acgcggctgc tggccggcga tcacgtgggc acctacttcg cgccgaaggc cgaccgcctg
     3961 accgcccgca agcgctggat cgccttcgcg gtgccgccgc agggccggct cacggttgac
     4021 gcgggcgccg tccgggcatt aa
//
LOCUS       DATIAZ010000323         7770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_621261, whole genome shotgun sequence.
ACCESSION   DATIAZ010000323 DATIAZ010000000
VERSION     DATIAZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621261"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VLF19_11195"
     CDS             complement(<1..196)
                     /locus_tag="VLF19_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93861.1"
                     /translation="MPRLRLMVSLFAFVLVLALVAGGSPAVSQTPKSGGVLNVMQRED
                     LAQGFSIHETATIATVWPAAP"
     gene            complement(353..1609)
                     /locus_tag="VLF19_11200"
     CDS             complement(353..1609)
                     /locus_tag="VLF19_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HSE93862.1"
                     /translation="MRTKRRLALAVLLAAVGLAAAAFPFRATWWGGWILAIAEAGIVG
                     GLADWFAVTAIFRRPLGLPIPHTGLIPRNWELLAARVGTMVGDRVLTREYLAHELARL
                     DLADLAARAAERLSRADLESATRAVLGWAVRAVPVESTGEITGRLQRLLVAQPVAPML
                     ARGLELARQHGWDQRAVGGAARAAGEAMDRPELRRAVEELVDEVLSRYRERMPLYPRL
                     ALGLADVLGLINRERVVGALRSGLEDVARDPAHPLRARVGEAIDALAARLRHDPAAAA
                     RAETVKAEMLGSPVVTRLAEDAARAVRRALLDDLERPQSEALGWLVDRLERWRRTLVA
                     DGELRRELDRWVKQRAQELVDRHHGLIAAFIEKGVHALGPEGAVRLIEEHAGDDLQYI
                     RVNGTVVGGLAGGLLYAIHLIIKSAW"
     gene            complement(1679..3403)
                     /locus_tag="VLF19_11205"
     CDS             complement(1679..3403)
                     /locus_tag="VLF19_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HSE93863.1"
                     /translation="MAHAERRRLTAVARGEAPADLFLRGGTLLNVYTGELYPAHVAAA
                     GERVAYVGSREDMVGPRTRVLDVAGRVLVPGYIDPHVHPAHLITPSALARHVLPLGTT
                     TVFADTLQIWELGGLPAFRATAGALARSPMKFYWMIRSHSQSRATDEAARFRLADLAR
                     ALGHPATVALGEVTRWPDAWRGEPEMLERLDLARRRRLRVEGHTAGASGEKIAAIAAA
                     GFTSDHESISAKEVLQRARQGIAVMLRESSLRPDLAGLLDALKEAPGLAGRVMLTADG
                     SMPAFVRDHGFVDHLLRVAMEGGVPPVDAYRMATLNAATYFGLDAEIGGVAPGRQADV
                     CVLRDLADPRPETVIARGRVVADAGRLTVAVPEPVWRRVLTSPEARLTVRWRARTDDF
                     ALPARERYPVVRLVSAVITRLEERAIGPGDLHGALIDRGGRWVAPAIVAGFGERIDGL
                     ASTISTDFNILVLGRRPESMARAVNRLLALRGGIVVVDGDSPAFELPLPLGGVMTPAS
                     LPETARLEDGLRAALMARGYPHHEPLFTLFFLSADFLPHVRLTPRGVWDVKQSRVLLP
                     ARRRARAR"
     gene            3509..3940
                     /gene="arfB"
                     /locus_tag="VLF19_11210"
     CDS             3509..3940
                     /gene="arfB"
                     /locus_tag="VLF19_11210"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HSE93864.1"
                     /translation="MAEAIVVTERVRVPARAITVRAVRSSGPGGQNVNKVASKVDLRV
                     DLRAIEGLPESALRRLEALASRRLDAQGRLVVTSQATRDQSRNLDDARSRVRALVAAA
                     LREPRVRRPTRPGLQAEERRIGAKKRRATVKRLRGRPVGDD"
     gene            complement(4025..4690)
                     /locus_tag="VLF19_11215"
     CDS             complement(4025..4690)
                     /locus_tag="VLF19_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93865.1"
                     /translation="MRSIAALVVLAVLIALPVGRSAPPDAFAQAAPAAAPRVWMQVDG
                     TVEKVEGDQMTFKLADGRRMVADISRMPGPDRTALVGGGGTTLYGYTDQQAGRFVALF
                     LPVEPAAAAAPGRPSVSPSSARFPVEPAGDERPWRIVHGRVDGIDSDTLTLITDAGRA
                     VTVDLRGVEPANRAAVARDDDVTVVGFHSGDLDRLDARYIHRDASESADQRAAAPSPY
                     RVR"
     gene            complement(4711..>7770)
                     /locus_tag="VLF19_11220"
     CDS             complement(4711..>7770)
                     /locus_tag="VLF19_11220"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HSE93866.1"
                     /translation="GGVLALGGDDPLSKSSTLPTHSEVAFYDALFPVLFPGSVQEVLD
                     LGRLGFELSRYSGLWVGFKIVTNVADEIGTAEVSQDRLVVRRPDLTFEGRPWQAQQAP
                     MLMPPFGLETERVIQYGRLEAARAFAAANGINRITVPTPGAWLGIAAAGKTYYDLREA
                     LRDLGLDDEALRRYGIRLLKIGMLFPMEPGIVHEFARGLEELLVIEEKRAFTELFIRD
                     VLYNLAERPRVVGKQDTDGRPLVPAHGELDADRIAQLVAARLERRVQSESITARVALL
                     EALRERPAPLTISRQPYFCSGCPHNRSTLVPEGSMASAGIGCHGMALYMDRNTSGLTH
                     MGGEGAQWVGMAPFTEMPHIFQNLGDGTLFHSGSLAIRQAVAAGTNVTYKILYNSAVA
                     MTGGQDAAGAMPVPELTRMLDAEGVRKVLVITDEPDKYPKGTRWAAGVEVWHRDRLDE
                     AQRMLRETPGVTALIYDQRCAAEKRRLRKRGKLPDPAMRVYINEAVCEGCGDCGVKSN
                     CLSVHPVETEFGRKTQIHQSSCNKDYSCLDGDCPSFLTVVPLGAPRKKQKPVFEASGE
                     LPEPALKVSRQCNVFMMGIGGTGVVTVNQILGTAALLDGKQVRGLDQTGLSQKGGPVV
                     SHLKIYERPEEVSNKVGAGEADTYIGFDILVATSPQNLDHARPDKTIAVVSTSKVPTG
                     AMVTSTEVQFPETGGLLTSINRVTRKDENVFLDALGLAERLFGDHMAANLIALGAAYQ
                     AGAIPVGATAIEEAIALNGVSVPMNTQAFRAGRLAVIAPEQVQTRPAQERPGAARVEL
                     SLHRHPELQSRVDEVAKGGELRRLLDVRVPELVAYQDVAYARQYVEFVKWVREAEQRA
                     VPGDTRLSEAVARHLYKLMAYKDEYEVARLHLANDLAAQLAAEYPDGVKVRYRLHPPL
                     LRALGLRRKIELGPWFNGALRLLVALRGLRGRALDPFGYAEVRRVERELIGEYRALVE
                     KSLVALSPESYERAVKLAGLPDLIRGYEDIKLRNVKRFRDEVRALGC"
BASE COUNT         1070 a         2796 c         2895 g         1009 t
ORIGIN      
        1 acggcgccgc cggccacacc gtcgcgatcg tcgccgtctc gtggatcgag aagccctggg
       61 cgaggtcttc gcgctgcatg acgttcagga cgccccccga tttcggcgtc tgactgacgg
      121 ccggcgatcc gccggcgacg agggcgagca ccagaacgaa cgcgaacaac gacaccatca
      181 ggcgaagacg gggcatgctg acctcccacg aagtgtcagg ttcctggcgg cttggtccgg
      241 tcgagtgtac gtgaaatccg gcgagccaac aattggttcc accatcgtgg ccgtcggagc
      301 gcccgcgtcg ggctccggcg ccggagagtc ggcggcagaa cgctcgagcg gctcaccacg
      361 cgctcttgat gatcaggtgg atcgcgtaca gcaggccgcc ggcgagaccg cccacgaccg
      421 tgccgttgac gcggatgtac tgcaggtcgt cccccgcgtg ctcctcgatc agccgcacgg
      481 cgccctccgg ccccagtgcg tgcacgccct tctcgatgaa cgccgcgatg aggccgtgat
      541 gacggtcgac cagctcctgg gctcgctgct tgacccagcg gtccagctcg cgccggagct
      601 cgccgtcggc cacgagcgtg cgccgccagc gctcgagccg gtcgaccagc cagccgagcg
      661 cctccgactg ggggcgctcc aggtcgtcga gcagcgcgcg ccggacggcg cgggcggcgt
      721 cctcggcgag ccgcgtgacg acggggctgc cgagcatttc cgccttcacc gtctccgcgc
      781 gcgccgccgc ggcggggtcg tggcgcagcc gggcggcgag ggcgtcgatg gcctcgccga
      841 cccgggcgcg gagcggatgc gccggatctc gggccacgtc ctcgagcccg ctgcgcagcg
      901 cgccgacgac gcgctcgcgg ttgatgaggc cgagcacgtc ggccagcccc agcgccagcc
      961 gcgggtagag cggcatgcgc tcgcgataac gcgacagcac ctcgtccacc agctcctcga
     1021 cggcccgccg cagctccggc cggtccatcg cctcgccggc cgcgcgcgcc gcgccgccga
     1081 ccgcccgctg gtcccagccg tgctggcgtg ccagctccag gccgcgcgcg agcatcggcg
     1141 ccaccggctg cgcgacgagg aggcgctgga gccggcccgt gatctcgccc gtggactcca
     1201 cgggcaccgc gcgcaccgcc cagcccagca cggcccgcgt ggccgactcg agatcggccc
     1261 ggctcagccg ttcggcggcg cgcgccgcga ggtcggcgag atcgaggcgc gccagctcgt
     1321 gcgcgagata ctcgcgggtg agcacgcggt cgcccaccat cgtgccgaca cgcgccgcga
     1381 ggagctccca gttcctgggg atcagcccgg tgtgcgggat cggcaggccc agcgggcgcc
     1441 ggaagatcgc cgtgaccgcg aaccagtcgg cgagaccgcc cacgatcccg gcctcggcga
     1501 tggccaggat ccacccgccc caccaggtgg cgcggaacgg aaaggccgcg gcggccagcc
     1561 cgaccgcggc cagcaggacg gcgagggcga gccgacgctt ggtcctcacg aggttgccgg
     1621 cgcggtccag gtcttcgtgg aggcctcgcg gggcgcgccc ccgacccccg ccgacgcgct
     1681 atcgtgcacg ggcgcggcgg cgcgcgggca gcaggacgcg ggactgcttg acgtcccaga
     1741 cgccgcgcgg cgtgagccgc acgtggggca ggaagtcggc ggagaggaag aagagcgtga
     1801 agagcggctc gtggtgcggg tagccgcggg ccatcagcgc cgcgcgcagt ccgtcctcga
     1861 gccgtgccgt ctccggcagt gaagccggcg tcatgacgcc gccgagcggc agcggcagct
     1921 cgaacgccgg gctgtcgccg tcgacgacca cgatgccgcc gcggagggcc aggagccggt
     1981 tgaccgcgcg cgccatcgac tccggccgcc ggccgagcac gaggatgttg aaatcggtcg
     2041 agatcgtcga ggcgagcccg tcgatcctct cgccgaagcc ggcgacgatc gcgggcgcca
     2101 cccaccgccc gccgcggtcg atcagcgctc cgtggaggtc gccggggccg atcgcacgct
     2161 cctcgagccg ggtgatgacc gcgctgacga gccggaccac ggggtagcgc tcgcgcgccg
     2221 gcagcgcgaa gtcgtccgtg cgagcgcgcc agcggacggt cagtcgggcc tcgggcgacg
     2281 tgagcacgcg ccgccagacg ggctccggca ccgccaccgt gagccggccg gcgtcggcca
     2341 cgacgcggcc gcgcgcgatc accgtctccg gacgcgggtc cgccaggtcg cgcagcacgc
     2401 agacgtcggc ctggcgcccc ggcgccacgc ctccgatctc cgcgtcgagg ccgaagtagg
     2461 tcgccgcgtt gagcgtggcc atgcgatagg cgtctacggg cggcacgccg ccctccatgg
     2521 cgacccgcag cagatgatcg acgaacccgt gatcgcggac gaaggccggc atcgagccgt
     2581 cggccgtcaa catgacgcgc ccggcgaggc cgggcgcctc cttgagcgcg tcgagcaggc
     2641 cggcgaggtc cgggcggagc gaggattcgc gcagcatgac ggcgatgccc tggcgggcgc
     2701 gctgcaggac ctccttcgcg ctgatcgact cgtggtcgga ggtgaagccg gcggcggcga
     2761 tggcggcgat cttctcgccg gacgcgcccg cggtgtggcc ctcgacgcgc agccggcgcc
     2821 gccgcgcgag gtcgagccgc tcgagcatct cgggttcgcc gcgccaggcg tcgggccatc
     2881 gcgtgacctc gcccagggcc acggtggcgg ggtgcccgag cgcgcgggcg aggtcggcga
     2941 ggcggaagcg cgccgcctcg tcggtagcgc gcgactgcga gtgcgagcgg atcatccagt
     3001 agaacttcat cggcgagcgc gccagcgcgc cggccgtggc gcggaacgcc ggcaggccgc
     3061 cgagctccca gatctgcagc gtgtcggcga agacggtcgt ggttcccagc ggcagcacgt
     3121 ggcgcgcgag tgccgagggc gtgatcaggt gcgcgggatg gacgtggggg tcgatgtagc
     3181 cggggacgag cacgcggccg gcgacgtcga gcacgcgggt gcgcgggccc accatatcct
     3241 cgcgtgaccc cacgtaggcc acgcgctcgc cggccgcggc gacgtgcgcc gggtagagct
     3301 cgcccgtgta cacgttgagc agggtgccgc cgcgcaggaa gaggtcggcg ggggcctcgc
     3361 cgcgcgccac cgccgtgagc cgccgccgct cggcgtgagc catctcgtgg ggattatccg
     3421 gtctcgcgtg cgggcgggca aatcggcgcg gtgccggcgc accgtcgagc acgcggggtc
     3481 gggcagccgt cgtggctaca atgggctcat ggccgaggcg atcgtcgtca ccgagcgggt
     3541 gcgcgtgccc gcccgcgcga tcaccgtgcg cgccgtgcgc tcctccggcc cgggcggtca
     3601 gaacgtcaac aaggtggcgt cgaaggtcga cctccgggtg gacctccgcg cgatcgaggg
     3661 cctcccggag tcggcgctcc ggcggctcga ggctctcgcg tctcgccgac tggacgccca
     3721 gggacggctc gtggtgacga gccaggccac gcgcgaccag tcacgcaacc tcgacgacgc
     3781 ccgatcccgg gtgcgcgcgc tcgtcgccgc cgcactcagg gagccgcggg tgcgacgacc
     3841 gacccggccc ggcctccagg ccgaggagcg gaggatcggc gccaagaagc gccgcgcgac
     3901 ggtcaagcgg ctgcgcggcc gtcctgtggg ggacgattag ggaggtgggg caaggcccga
     3961 catggcgccg ggcgcgcccg gccccgccgc gatgccccgg cgggcacctc ctggcgattc
     4021 gcgctcagcg cacgcgataa gggctgggcg ccgccgcgcg ctggtccgcc gactcggacg
     4081 cgtcgcggtg gatgtagcgc gcgtcgaggc ggtcgaggtc gcccgagtgg aagccgacca
     4141 cggtcacgtc gtcatcccgc gcgacggcgg cgcggttggc gggctcgaca ccgcgcaggt
     4201 ccaccgtgac cgcgcgtccg gcgtcggtga tcagcgtcag cgtgtcggag tcgattccgt
     4261 ccacacggcc gtgaacgatg cgccaggggc gctcgtcccc cgcgggctcg acggggaatc
     4321 gcgccgagga cggtgagacg ctcggcctgc ccggcgccgc ggcggccgcg ggctcgacag
     4381 gcaagaacaa ggcgacgaac cggcccgcct gctggtccgt gtagccgtag agtgtcgtgc
     4441 ccccgccgcc gaccagcgcg gtgcggtccg gtccgggcat gcgcgagatg tccgccacca
     4501 tgcgacggcc atcagcgagc ttgaacgtca tctggtcgcc ctcgaccttc tccacggtgc
     4561 cgtccacctg catccacacg cgtggcgcgg ctgccggagc cgcctgggcg aaggcgtcag
     4621 gaggagcgga acgcccgacg ggcagggcga tcaggacggc gagcacgacg agggcggcga
     4681 tggaacgcat ggcagtctcc tgttgccggc tcagcatccg agcgcgcgga cctcgtcgcg
     4741 gaagcgcttc acgttccgga gcttgatgtc ctcgtagccg cgaatcaggt cgggcaggcc
     4801 ggccagcttc acggcccgct cgtagctctc gggcgacagg gcgacgagcg atttctcgac
     4861 cagggcccgg tactcgccga tcagctcgcg ctcgacgcgg cggacctcgg cgtagccgaa
     4921 cgggtccagc gctcgaccgc gcaggccgcg cagcgccacc agcaggcgca aggcgccgtt
     4981 gaaccacggg ccgagctcga tcttgcgccg gagcccgagc gcgcgcagca gcggcgggtg
     5041 caggcggtac cggaccttca cgccgtccgg gtactccgcg gccagctggg cagccagatc
     5101 gttggcgagg tgcaggcggg ccacctcgta ctcgtccttg taggccatga gcttgtagag
     5161 atggcgggcc acggcctcgg agagccgggt gtcgccgggc accgcgcgct gctcggcctc
     5221 cctcacccac ttgacgaact ccacgtactg gcgcgcgtac gcgacgtcct ggtaggcgac
     5281 gagctcgggc acgcggacgt cgaggagccg ccgcagctcg ccgcccttcg cgacctcgtc
     5341 gacgcgcgac tgcagctccg gatgccggtg gagcgacagc tcgacgcggg ccgcgcccgg
     5401 ccgctcctgc gctggccgcg tctgcacctg ctccggagcg atcacggcca gccgcccggc
     5461 ccggaaggcc tgggtgttca tcgggacgga gacgccgttg agggcgatcg cctcctcgat
     5521 ggccgtggcg ccgaccggga tggcgcccgc ctggtaggcg gcgccgagcg cgatcaggtt
     5581 ggcggccatg tggtcgccga agagccgctc cgcgagcccg agggcgtcga ggaagacgtt
     5641 ctcgtccttc cgcgtgacgc ggttgatcga ggtcaggagc ccacccgtct cggggaactg
     5701 cacctccgtg gacgtcacca tcgccccggt cggaaccttg ctcgtgctga cgacggcgat
     5761 cgtcttgtcc ggacgcgcgt ggtcgaggtt ctgcggggac gtggcgacga ggatgtcgaa
     5821 gccgatgtag gtgtcggcct cgcccgcccc gaccttgttc gagacctcct ccggccgctc
     5881 gtagatcttg agatgcgaga ccaccggccc gcccttctgg ctgagcccgg tctggtcgag
     5941 cccgcgcacc tgcttgccgt cgagcagggc cgcggtgccc aggatctggt tcacggtcac
     6001 cacgccggtg ccgccgatgc ccatcatgaa gacgttgcac tggcgcgaga ccttgagcgc
     6061 cggctcgggc agctcgccgg acgcctcgaa cacgggcttc tgcttcttcc tcggcgcgcc
     6121 gagcggcacc acggtgagga acgacgggca gtcgccgtcg aggcacgagt agtccttgtt
     6181 gcaggacgac tggtggatct gggtcttgcg gccgaactcg gtctcgaccg gatggacgct
     6241 gaggcagttc gacttcacgc cgcagtcacc gcatccctcg cagacggcct cgttgatgta
     6301 cacgcgcatc gccggatccg gcagcttgcc gcgcttgcgc agccggcgct tctccgccgc
     6361 gcagcgctgg tcgtagatca gcgccgtcac gcccggcgtc tcgcgcagca tccgctgggc
     6421 ctcgtcgagc cggtcgcggt gccacacttc cacgccggcg gcccaccgcg tgcccttcgg
     6481 gtacttgtcg ggctcgtcgg tgatcacgag gaccttccgc acgccctcgg catcgagcat
     6541 tcgcgtcagc tcgggcaccg gcatggcgcc ggcggcatcc tgcccgccgg tcatggccac
     6601 ggccgagttg tagaggatct tgtacgtgac gttggttccc gccgcgaccg cctggcggat
     6661 ggcgagcgag cccgagtgga agagcgtgcc gtcaccgagg ttctggaaga tgtgcggcat
     6721 ctccgtgaac ggcgccatgc cgacccactg cgcgccctcg ccgcccatgt gcgtgagccc
     6781 ggaggtgttg cggtccatgt agagcgccat gccgtggcag ccgatgccgg ccgacgccat
     6841 ggagccctcg ggcaccagcg tggagcggtt gtgggggcag cccgagcaga agtagggctg
     6901 gcgcgagatc gtgagcggcg cggggcgctc gcggagcgcc tcgagcaggg ccacgcgcgc
     6961 ggtgatcgac tcgctctgca cgcgccgctc gagccgcgcg gccacgagct gggcgatgcg
     7021 gtcggcgtcg agctcgccgt gggcgggcac gagcgggcgg ccgtcggtgt cctgcttgcc
     7081 gaccacccgg ggacgctccg ccaggttgta gagcacgtcc cggatgaaca gctccgtgaa
     7141 cgcgcgcttc tcctcgatga ccaggagctc ctcgagcccg cgcgcgaact cgtgcacgat
     7201 gccgggctcc atcgggaaca gcatgccgat cttgagcagc cggatgccgt agcggcgcag
     7261 ggcctcgtcg tcgaggccga ggtcgcgcag cgcctcgcgc aggtcgtagt acgtcttgcc
     7321 ggccgcggcg atgccgagcc acgccccggg cgtcggcacg gtgatgcggt tgatcccgtt
     7381 ggccgcggcg aaggcccggg cggcttcgag ccggccgtac tggatcaccc gctcggtctc
     7441 caggccgaag ggcggcatca gcatgggcgc ctgctgcgcc tgccaggggc ggccctcgaa
     7501 ggtgaggtcg ggacggcgca cgaccagccg gtcctggctc acctcggcag tgccgatctc
     7561 gtcggcgacg ttggtcacga tcttgaaacc gacccagagc cccgagtacc ggctgagctc
     7621 gaagccgagc cggccgaggt cgagcacctc ctggacgctg ccagggaaga gcaccgggaa
     7681 gagcgcgtcg tagaacgcca cctccgagtg cgtcggcagc gtggaggact tcgagagcgg
     7741 gtcgtcgcca ccgagcgcga gcacgccgcc
//
LOCUS       DATIAZ010000324         6905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1673701, whole genome shotgun sequence.
ACCESSION   DATIAZ010000324 DATIAZ010000000
VERSION     DATIAZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673701"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1033)
                     /locus_tag="VLF19_11225"
     CDS             complement(26..1033)
                     /locus_tag="VLF19_11225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSE93867.1"
                     /translation="MLQVGVLRGLLAWLLACGAFMGLEAAGLAEGLALPVPLIAALAL
                     ARALGESAWARRHGALVAAVVALGAAALGLAAWWCGLAPPAFSSGETAAALLAGAGAT
                     SLLAPRAVRAALMPPLGLDPGSPVHAVVLVAGVATVASAAMLFVELRDEAAASLPFYA
                     TDSVVAVVTDAALALAGVGFLLTRDGRAARERLDLRPLGPRLAAWALATALGFHVVVG
                     ALEWTESVLFPELHALEDRFDYDFVGIPPLIGAVLVSVAAGVGEELLFRGALQPRVGV
                     VLSAALFAALHVQYQVPGVLMIFAVGLALGVLKQRTSTTFTMLVHVVYDLGAFLLDFL
                     E"
     gene            1121..1630
                     /locus_tag="VLF19_11230"
     CDS             1121..1630
                     /locus_tag="VLF19_11230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017688107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE93868.1"
                     /translation="MGDTGPNVTLHEASAADVPVLRRLMQLYLYDLGTIDGWDIGEDG
                     LFGSAERIESFWTEDGRRTLLIRVDGVLAGFLMIRDGGRFSGPGTRELAEFFVLRKYR
                     RRGVGTRAATAVFDMLPGRWEVDQLASNAEAQAFWRAVIGRYTGGRFDDAERRLGTWR
                     GRVQHFHAP"
     gene            1715..2536
                     /locus_tag="VLF19_11235"
     CDS             1715..2536
                     /locus_tag="VLF19_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HSE93869.1"
                     /translation="MTMSQSQKATRFRALHERPDAFVIPNPWDAGSARILAALGFQAL
                     ATSSGAHAGVLGRRDGRVTRDEALAHARAIVEATDLPVSADLEKGFGDAPTVAAESIR
                     LAAGVGLVGGSIEDATGDKDRPLYDLGHAIERVAAAVEAARSLPFPFTLTARTENFLR
                     GNPSLDDTIRRLQAFEQAGADVLMAPGLPDLAAVRAVCAAVSRPVNFMAGIKGKSFTV
                     AELAAAGVRRISLATSLYRAAMTGLLDAAREVSDKGTFGYLERSVTTPDLNAFMS"
     gene            2624..3358
                     /locus_tag="VLF19_11240"
     CDS             2624..3358
                     /locus_tag="VLF19_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93870.1"
                     /translation="MLEGSGITMRFGGLAALTNVGVQVAPGEIVGLIGPNGAGKTTLF
                     NAISGLIPLGAGAVRLDGRDITGLPAHRIARLGIGRTFQSPRLFPRLTTFEHVLLSAR
                     SRPRGEASLRTKEGDEGEARRALEFVRLEHRASFEAGREPTGRRKLVELAMVLAARPR
                     VLLIDELMAGLNPGEVQFAMRLIRRIRDERGAAILWVEHVMEAIMGVADRVVVLHHGE
                     KIAEGTPAAVAEDPRVLDAYLGERAA"
     gene            3403..4074
                     /locus_tag="VLF19_11245"
     CDS             3403..4074
                     /locus_tag="VLF19_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE93871.1"
                     /translation="MLWEVSLGVGPGEVVALVGSNGAGKTTLLRILSGLSEGFLRVTR
                     GGVRFRGETLAGLDPAQIVTRGIAHVPEGRHLFPGLTVRENLRLGGYLLPSRSQARES
                     LERVFGMLPFLRERQQQLAGTLSGGEQQLLAIGRALMQRPSLLMLDEPSVGLAPRMVA
                     VVFDLVARIRAAGVSVLLVEQNVRQALGLADRGYVLENGRIVLEGPGAQLVEHPTLKR
                     AYLGL"
     gene            4115..5326
                     /locus_tag="VLF19_11250"
     CDS             4115..5326
                     /locus_tag="VLF19_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HSE93872.1"
                     /translation="MDTLIGRRSFLKITGATAAASMVARPARAQGPIRVGFTLSATGP
                     YAVGAGITQRPNYDLWLEQVNAKGGLAVAAQGRRPIQFVDHDDRSEIETAVRLYEKMI
                     TDDKVDLVLPPWGTAMNFAVGPIANKHGYPMIGPTVGSLKLKDLNLPYFYAVLAQPDA
                     MMASLAAFLRDVKHQQRLNKVAVIYVNDLFGLEHHGALMPLLKEGDFEVVEVKSYPLG
                     VKDLTDVLKGIKAGGAEILVGLSYPPDTILITTQAKAVDFNPAIFYTAVGTAFPFFRD
                     RFKGSAEGVMGVGAWNPKVKYPGARAYFDAHVARHKKEPDRWASAFAYATLQILEESV
                     GKVGLDRARIKAHLDATEFPTVVGPIKFVKGVNTATPGMVGQWQKGEFEVVWPLERAT
                     AKAGVPKPKWE"
     gene            5337..6245
                     /locus_tag="VLF19_11255"
     CDS             5337..6245
                     /locus_tag="VLF19_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93873.1"
                     /translation="MNLALLLDVAAGGLLLGGLYALVAVGLNLQYGLMRVLNVAHGEF
                     LMIGGYVTYLLHVNTGMDPLVSLLVSGPLVFVAGVAVHTLLYRRLLRQERTREELEAN
                     SLLISFGLLFILQNGALLLFSADIRGYQYLDRPLRMLGGVFPANRVVAFLMAAVITAL
                     LFLFLRRTLAGAAVRALMQDPVGARLVGVRVPRVHALCFGLGAGLAAVSGSLLSMLFE
                     LTPFVGLPYTITALVVVILGGLGSVGGSLLGGLILGVVETLGVYFTAPDLRMVISYGV
                     LAIVLVLRPYGLFNPMARRAGQSERA"
     gene            6324..>6905
                     /locus_tag="VLF19_11260"
     CDS             6324..>6905
                     /locus_tag="VLF19_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE93874.1"
                     /translation="MWGIALLAALVVFPLVARAYHVALGLALLADVALASAWALFSGP
                     TRYLSLAAAAFYGVGAYVTALLGAGTAWPLPVLAGAAAGALLSLPVGLLALRLRGPYF
                     AILTFGLSELVRHTVIWYESNVTGTVGRVLVLALERRTLYWGLLVLAVLSVTAAILLR
                     RSRWGLALVAIGGDEERAEVLGVRAIGVKIAVFA"
BASE COUNT          945 a         2438 c         2527 g          995 t
ORIGIN      
        1 ggctgtcgag acctcctccg aggagctatt ccaggaaatc gaggaggaac gcgccgagat
       61 cgtagacgac gtggacgagc atcgtgaacg tcgtcgaggt ccgctgcttg agcacgccga
      121 gcgcgagccc cacggcgaag atcatgagga ccccgggcac ctggtactgc acgtggaggg
      181 cggcgaagag cgccgcgctg agcaccacgc ccacgcgcgg ctggagcgcg cctcggaaga
      241 gtagctcctc gcccaccccg gccgcgaccg acacgagcac cgcgccgatc agcggcggga
      301 tgccgacgaa gtcgtagtcg aagcggtcct cgagcgcgtg cagctccggg aacagcacgc
      361 tctccgtcca ttcgagcgcg cccaccacca cgtgaaagcc gagcgcggtg gcgagcgccc
      421 aggcggcgag gcgcggcccc agcggccgca ggtcgagccg ctcccgcgcg gcacgcccgt
      481 cgcgcgtcag gaggaacccc actccggcca gcgccagcgc ggcgtcggtc accacggcca
      541 ccacggagtc ggtggcgtag aacggcaggc tcgccgccgc ctcgtcgcgg agctcgacga
      601 agagcatcgc cgccgaggcg accgtcgcga cgccggcgac caggacgacc gcgtgtaccg
      661 gcgagccggg atcgagcccg agtggcggca tgagcgccgc gcggaccgcc cgcggcgcca
      721 gcagcgaggt cgcgcccgcg ccggcgagga gcgccgcggc ggtctcgccg gacgagaagg
      781 ccggcggcgc caggccgcac caccacgccg cgagcccgag cgccgccgcg ccgagcgcga
      841 cgaccgcggc gacgagcgcc ccgtggcggc gcgcccacgc cgactcgccg agcgcgcgcg
      901 cgagcgcgag agccgcgatg agcggaaccg gcaacgcgag gccctccgcg agccccgccg
      961 cctccagccc catgaacgca ccgcacgcga ggagccaggc gaggaggcca cggagcacgc
     1021 ctacttgtag catcgctcac cgtccaccgg tgagaggtcc cggctggcgc cgcgagccgc
     1081 gcggcccgcc ggcgggcaat ggcgatatcc tcggaccgtc gtgggagaca ccggcccgaa
     1141 cgtgacgctc cacgaggcca gcgcggcgga cgtcccggtc ctgcgccggc tcatgcagct
     1201 ctacctctac gatctcggca cgatcgacgg ctgggacatc ggcgaggacg gcctcttcgg
     1261 cagcgccgag cggatcgaga gcttctggac ggaggacggc cggcgcacgc tcctgatccg
     1321 cgtggacggg gtcctggcgg gcttcctgat gatccgcgac ggcggccggt tctccggccc
     1381 cggcacacgc gagctggcgg agttcttcgt gctgcggaag tatcgtcgcc gcggagtcgg
     1441 cacgcgcgcg gcgacggccg tcttcgacat gctcccgggc cgctgggagg tcgatcagct
     1501 ggcgagcaac gcggaggcgc aggccttctg gcgggcggtg atcggccgtt acacgggcgg
     1561 ccgcttcgac gacgccgagc gtcggctcgg aacctggcgc ggccgggtcc agcatttcca
     1621 cgccccgtga accgtgcgct cgcgttcgtg taccctctcg gcgcgaggcc ttccacgtcg
     1681 agggcaggcc gatcccgacc caggaggaag tgggatgacg atgagccaga gccagaaagc
     1741 tacgcgcttc cgggccctcc acgagcgccc cgacgccttc gtgatcccga atccgtggga
     1801 tgccggctcg gcgcgcatcc tcgccgcgct cggcttccag gcgctggcga cgtcgagcgg
     1861 tgcccacgcc ggcgttctcg gccggcgcga cggccgggtg acgcgcgacg aggcgctggc
     1921 acacgcccgc gcgatcgtgg aggcgaccga ccttccggtc tcggcggatc tggagaaagg
     1981 cttcggcgac gcgccgacgg tcgcggcgga gagcatccgc ctcgccgccg gggtgggcct
     2041 ggtcggtggc tcgatcgagg acgcgacagg cgacaaggac cggccgctct acgacctcgg
     2101 ccacgcgatc gagcgcgtgg cggcggccgt ggaggcggcg cgctcgctcc ccttcccgtt
     2161 cacgctgacg gcgcggaccg agaacttcct gcgcggcaac ccgagcctcg acgacacgat
     2221 caggcgcttg caggccttcg agcaggcggg cgccgacgtg ctcatggcgc cgggcctgcc
     2281 cgatctggcg gcggtgcgcg ccgtgtgcgc agcggtctcc aggccggtca acttcatggc
     2341 cggcatcaag ggcaagtcgt tcacggtggc cgagctggcg gcggccggcg tccggcggat
     2401 cagcctcgcc acctcgctct atcgagcggc gatgacgggg ctgctcgacg ccgcgcgcga
     2461 ggtgagcgac aagggcacgt tcggctatct cgagcgctcc gtcaccacgc cggacttgaa
     2521 cgcattcatg tcgtgaggga gcggcgccgc ttgacgcggg cggtccgctg atgttacctg
     2581 cctgtgcttc gcggctcggg acggccgtca ccaagggggc ggtgtgctcg aaggcagcgg
     2641 catcaccatg cggttcggcg ggctggcagc gctcacgaat gtcggtgtcc aggtcgcacc
     2701 cggggagatc gtcgggctca tcgggccgaa cggggccggc aagaccacgc tgttcaacgc
     2761 gatcagcgga ctgatcccgc tcggcgcggg cgccgtgcgc ctggacggcc gtgacatcac
     2821 cgggctgccg gcccaccgca tcgcgcgcct cgggatcggg cgcacgttcc agtcgccccg
     2881 gctcttccct cgtctcacga cgttcgagca cgtcctgctg agcgcccggt cccgcccgcg
     2941 gggggaggcc tcgctgcgca ccaaggaggg cgacgagggc gaggcccggc gcgcgctgga
     3001 attcgtgcgc ctggagcatc gggcctcgtt cgaggccggg cgcgagccga cgggacggcg
     3061 caagctggtg gagctcgcga tggtcctggc cgcccggccg cgcgtcctcc tgatcgacga
     3121 gctgatggcc ggcctcaacc ccggcgaggt ccagttcgcc atgcgcctga tccgccgcat
     3181 ccgcgacgag cgaggcgcgg cgatcttgtg ggtggagcac gtcatggagg cgatcatggg
     3241 ggtggccgac cgcgtggtgg tgctccacca cggcgagaag attgccgagg gaacgccggc
     3301 cgcggtggcc gaggatcccc gcgtcctcga cgcctatctc ggggagcgtg cggcctgatc
     3361 ctggaggtcg agagggtcca ggccgcgtac cgggacgtcc cggtgctctg ggaggtctcg
     3421 ctcggcgtcg gccccggcga ggtcgtggct ctggtcgggt cgaacggcgc gggcaagacc
     3481 acgttgctga ggatcctgtc gggcctgagc gagggattcc tgcgcgtgac gcgcggcgga
     3541 gtgcgattcc gcggcgagac gctcgccggg ctcgatcctg cccagatcgt gacgcggggc
     3601 atcgcccacg tcccggaggg gcggcacctc ttcccggggc tcacggtgcg ggagaacctc
     3661 cgcctgggcg gttacctgct cccgtcgcgg agccaggcgc gcgagtcgct cgagcgggtg
     3721 ttcgggatgc tgccgttcct gcgcgagcgg cagcagcagc tggcggggac gctctcgggg
     3781 ggcgagcagc agctgctggc catcggccga gccctcatgc agcggccgtc gctcctgatg
     3841 ctcgacgagc cgtcggtggg gctcgccccg cgcatggtcg cggtggtctt cgacctggtg
     3901 gcacgcatcc gcgccgcagg ggtgagcgtg ctgctcgtgg agcagaacgt ccgccaggcg
     3961 ctcgggctcg cggatcgcgg ctacgtgctg gagaatgggc ggatcgtcct ggaagggccg
     4021 ggggcccagc tggtcgagca ccccacgctg aagcgggcct atctcgggct gtgaacgcgg
     4081 cgcggcccca gggctccgcc gggaggagaa cagcatggac acgctgatcg ggcgcaggag
     4141 cttcctgaag atcaccgggg ccacggccgc cgcgagcatg gtggcgcgcc ccgcccgggc
     4201 gcaggggccg atccgggtcg gcttcacgct ctcggccacc ggaccctacg cggtcggcgc
     4261 ggggatcacg cagcggccca actacgacct gtggctcgag caggtcaacg ccaagggcgg
     4321 cctcgccgtg gcggctcagg gccggcggcc catccagttc gtggaccacg acgaccgcag
     4381 cgagatcgag accgcggtcc ggctctacga gaagatgatc accgacgaca aggtcgacct
     4441 cgtgctgccg ccctggggca cggccatgaa cttcgccgtc ggcccgatcg ccaacaagca
     4501 cgggtacccg atgatcggcc ccacggtggg ctcgctcaag ctcaaggacc tgaacctgcc
     4561 gtacttctac gccgtgctcg cccagcccga cgcgatgatg gcctcgctgg ccgccttcct
     4621 cagggacgtg aagcaccagc agcgcctcaa caaggtcgcc gtgatctacg tgaacgacct
     4681 cttcggcctc gagcaccacg gcgcgctgat gccgctcctg aaggaaggcg acttcgaggt
     4741 ggtggaggtc aagagctacc cgctcggcgt gaaggacctg accgacgtgc tcaagggcat
     4801 caaggccggg ggcgccgaga tcctcgtggg gctcagctat cctcccgaca ccatcctgat
     4861 cacgacgcag gccaaggcgg tggatttcaa ccccgcgatc ttctacaccg cggtggggac
     4921 ggcgttcccg ttcttccgcg accggttcaa gggatcggcc gagggggtca tgggcgtggg
     4981 cgcctggaac ccgaaggtga aataccccgg cgccagggcc tacttcgacg cgcacgtcgc
     5041 caggcacaag aaggagcccg accgctgggc cagcgccttc gcctacgcga cgctgcagat
     5101 cctcgaggag agcgtcggca aggtggggct cgatcgcgcc aggatcaagg cccacctcga
     5161 cgccaccgag ttcccgacgg tcgtgggacc gatcaagttc gtcaagggcg tgaacacggc
     5221 gacgccgggc atggtaggac agtggcagaa gggcgagttc gaggtggtgt ggccgctcga
     5281 gcgggcgacg gcgaaggctg gcgtgccgaa gcccaagtgg gagtgacggg gcccgcgtga
     5341 acctggcgct gctgctcgac gtggcggcgg gaggtctcct gctcggaggc ctctacgccc
     5401 tcgtcgccgt gggtctcaat ctgcaatacg ggctcatgcg cgtgctcaac gtggcccacg
     5461 gcgagttcct gatgatcggc ggctacgtga cgtacctgct ccacgtgaac acggggatgg
     5521 atcccctcgt ctccctcctg gtcagcggac cgctcgtctt cgtggccgga gtggccgtgc
     5581 acacgctcct ctaccggcgg ctgctgcgtc aggagcggac acgggaggag ctggaagcca
     5641 actcgctgct gatctccttc ggcctcctct tcattctcca gaacggcgcg ctgctgctct
     5701 tcagcgccga catccgcggc taccagtacc tcgaccggcc cctgcgcatg ctcggcggcg
     5761 tgttccccgc caaccgggtg gtcgccttcc tcatggccgc ggtgatcacc gcgctcctct
     5821 tcctcttcct gcgccgcacg ctggccggcg ccgcggtgcg ggccctcatg caggatcccg
     5881 tgggcgcgcg gctcgtcggc gtgcgcgttc cgcgggtcca cgcgctgtgc ttcgggctgg
     5941 gggcgggcct ggccgcggtc agcggatcgc tgctcagcat gctcttcgag ttgacgccgt
     6001 tcgttggcct gccctacacg atcaccgcgc tggtggtggt catcctgggc gggctcggca
     6061 gcgtcggcgg cagcctgctc ggcggcttga tcctcggcgt cgtggagacg ctcggcgtct
     6121 acttcaccgc ccccgacctc cgcatggtga tcagctacgg cgtgctcgcc atcgtcctcg
     6181 ttctccggcc gtacggtctc ttcaacccca tggctcgccg cgccggtcaa tcggagcggg
     6241 cgtagcgcgc gatggacctc tcgggaggac cgggccggct ctggcacagg atccgccggg
     6301 ggctgcgagg gaagcgggca tgggtgtggg ggatcgctct cctcgcggcc ctcgtcgtct
     6361 tcccgctcgt ggcgcgcgcc taccacgtcg ccctcggtct cgccctgctc gcggacgtgg
     6421 cgctggcctc ggcgtgggcg ctcttctcgg ggccgacgcg gtatctctcg ctggcggccg
     6481 ccgccttcta cggcgtcggc gcttacgtga cggcgctcct cggcgcgggc accgcgtggc
     6541 cgttgcccgt cctggccggc gccgccgccg gcgcgctcct ctcgctgccg gtcgggctgc
     6601 tcgcgctgcg gttgcgcggg ccgtacttcg cgatcctgac ctttggcctc agcgagctgg
     6661 tccgccacac ggtgatctgg tacgagtcca acgtcaccgg caccgtcggg cgcgtgctcg
     6721 tgctggcgct cgagcggcgg acgctctact ggggtctgct cgttctcgcc gtcctctccg
     6781 tgacggcggc gatccttcta cggcggtcgc ggtggggact ggcgctcgtg gccatcggcg
     6841 gcgacgagga gcgggccgag gtcctcggcg tgcgggccat cggggtcaag atcgccgtct
     6901 tcgcg
//
LOCUS       DATIAZ010000325         4309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1146541, whole genome shotgun sequence.
ACCESSION   DATIAZ010000325 DATIAZ010000000
VERSION     DATIAZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146541"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /locus_tag="VLF19_11265"
     CDS             <1..2186
                     /locus_tag="VLF19_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE93875.1"
                     /translation="FTGKDMAGVNSLPCGWDLRRAKNIPGVVQDLAIVPHMPLTGDVA
                     RHVGDPVAVVIADSQGAAVDAAEKVRVDWEPLPSVTATDRATVSGVAQVHADAAGNVA
                     FKWALGDAAATDAAFRSAATTVKKHIVNQRLVANAMEPRACAARFDPATGELTLWVTS
                     QNPHVHRLLMCAFVLGIPEHKVRVIAPDVGGGFGSKIFLYNEEVVCSWASRQLQRPVR
                     WTATRREAYQTDAHGRDHITDAELALSRDGKILGLRVKTVANLGAYLSTFAPAVPTFL
                     YGTLLNGVYAIGAIHVEVTGVFTNTTAVDAYRGAGRPEACYVLERMVDVGARALNMDV
                     AEIRRKNFIPKFSGAFQTHVAVTYDSGDYAAAFDKLLQIFDYKKFRAEQADARKQGRL
                     LGVGFSTYIEACSIAPSKVVGALGAGAGLYESGKVRVHPTGMVTVFTGSHSHGQGHET
                     TFAQLVADDLGIPIEQVEIVHGDTGTVPFGMGTYGSRSASVGGTAIKMSLDKIREKGT
                     RIAAHLLEASPKDIEYANGEFRVRGVPGKTVPFGAVALTAYVPHNYPEGLEPGLEETS
                     FYDPSNFCFPFGAHACVVEIDPDTGNVKLRRYVAVDDVGNVINPMIVDGMVHGGIAQG
                     VAQALWEGAVYDGQSGQLVTSSMMDYAVPKAEMLPNYETDRTVTPTPVNSLGVKGAGE
                     TGTIAATPAVVNAVVDALASAGVDHIETMPLTAERVWKTIQAARR"
     gene            2318..3187
                     /locus_tag="VLF19_11270"
     CDS             2318..3187
                     /locus_tag="VLF19_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSE93876.1"
                     /translation="MYPAAFEYHAPTSVQDALGLLGRLKDDAKLLAGGHSLVPMMKLR
                     LAQPKHIIDLRKVPGLAGIKEEGGAIVIGAMTTHWQMESSPLLKQKCPILSETASIIG
                     DPAVRNVGTIGGSLAHADPAADQPATMIALGVEFVCQGPKGRRTVKVDDWFQGLMSTA
                     VGEEELLVEIRVPAWPAGTGGAYMKFPHPASRFAVVGVGAVVTLDGEGRCTRAGVGVT
                     GAGTKAVRAKGVEAGLVGKKLDAAAIEAAAAKAHEGVDVQADLQGSVEYKSHLCRVFA
                     KRAITEAAKRARA"
     gene            3477..4052
                     /locus_tag="VLF19_11275"
     CDS             3477..4052
                     /locus_tag="VLF19_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSE93877.1"
                     /translation="MIAGIVLAAGFARRMGRQKLLLAYRGAPIVRLSVEALARHVEDV
                     VVVTGQDEGAVREALAGLDVRFARNPRPHDGLGSSIAVGVAALKPWTTATLVALGDQP
                     HVPAPVVPALLATFGRGGKAIVAPVYRGTQGTPVLFGSEVFAELSALTGDSGARAVVA
                     SRPDRVALVPFDLPMPADVDTPEDFARLHVE"
     gene            4092..>4309
                     /locus_tag="VLF19_11280"
     CDS             4092..>4309
                     /locus_tag="VLF19_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSE93878.1"
                     /translation="MEGAEYVTDAPVATSVHLALALPKPLLIEGHAGVGKTEVAKVMA
                     RVLGTNLIRLQCYEGLDVSTALYEWNYQ"
BASE COUNT          672 a         1483 c         1537 g          617 t
ORIGIN      
        1 tcttcaccgg caaggacatg gccggcgtca actcgttgcc gtgcggctgg gatctccgcc
       61 gggccaagaa catcccgggc gtggtccagg acctcgccat cgtgccccac atgccactga
      121 ccggggacgt tgcccgacac gtcggtgatc cggtggcggt ggtcatcgcc gacagccagg
      181 gggcggcggt ggacgcggcc gaaaaggtgc gcgtggactg ggagccgctg ccctcggtga
      241 cggcgaccga cagggctacc gtgtccgggg tcgcgcaggt ccacgccgac gcggcgggca
      301 acgtcgcgtt caagtgggcc ctcggcgatg ccgcggccac cgacgcagcc ttcaggagcg
      361 cggccacgac ggtgaagaag cacatcgtca accagcggct ggtcgccaac gccatggagc
      421 cccgcgcctg cgcggcccgg ttcgacccgg ccaccggcga gctcacgctg tgggtcacct
      481 cgcagaaccc ccacgtgcac cggctcctga tgtgcgcctt cgtcctcggt atccccgagc
      541 acaaggtgcg ggtgatcgcg cccgacgtcg gcggcggctt cgggtcgaag atcttcctct
      601 acaacgagga agtcgtctgc tcgtgggcgt cgcgccagct gcagcggccg gtccgctgga
      661 cggccacccg ccgggaggcg tatcagaccg acgcgcacgg ccgcgaccac atcaccgacg
      721 cggagctggc gctgtcgcgc gacggcaaga tcctcggcct gcgcgtgaag acggtggcca
      781 acctcggcgc ttacctgtcc acgttcgcgc cggcggtgcc gacgttcctc tacggcacgc
      841 tgctgaacgg cgtgtacgcg atcggtgcca tccacgtgga agtcaccggc gtgttcacca
      901 acaccacggc cgtcgacgcc taccggggcg ccggccggcc ggaggcctgc tacgtcctcg
      961 agcggatggt cgacgtcggg gcccgtgccc tgaacatgga cgtcgcggag atccgtcgca
     1021 agaacttcat cccgaagttc tccggcgcct tccagacgca cgtcgccgtg acctacgaca
     1081 gcggcgacta cgcggcggcc ttcgacaagc tcctgcagat cttcgactac aagaaattcc
     1141 gggccgagca ggcggacgcc cgcaagcagg ggcggctgct cggcgtcgga ttctcgacgt
     1201 acatcgaggc gtgctcgatc gccccgtcga aggtcgtggg cgccctcggc gcgggtgccg
     1261 gcctctacga atccggcaag gtgcgcgtcc acccgaccgg gatggtcacc gtcttcacgg
     1321 gctcgcactc gcacggccag gggcacgaga ccaccttcgc ccagctcgtg gccgacgacc
     1381 tcggcatccc catcgagcag gtcgagatcg tccacggcga caccggcacc gtccccttcg
     1441 gtatgggcac ctacggcagc cgctcggcgt ccgtgggcgg aaccgcgatc aagatgtcgc
     1501 tcgacaagat ccgtgagaag ggcacgcgga tcgccgcgca cttgctggag gcgtcgccga
     1561 aggacatcga gtacgcgaac ggcgagttcc gggtgagggg cgtgcccggc aagacggtgc
     1621 cgttcggcgc cgtggcgctg accgcgtacg tgcctcacaa ctaccccgag gggctcgagc
     1681 cgggtctcga ggagacgagc ttctacgatc cttcgaactt ctgcttcccg ttcggcgctc
     1741 acgcgtgcgt ggtcgagatc gatcccgaca ccggcaacgt gaagctgcgc aggtacgtgg
     1801 cggtggacga cgtcggcaac gtcatcaacc ccatgatcgt ggacggcatg gtgcacggcg
     1861 gcatcgccca gggtgtcgcc caggcgctgt gggagggcgc ggtctacgac ggccaatcgg
     1921 ggcagctcgt gacctccagc atgatggact acgccgtccc caaggccgag atgctcccca
     1981 actacgagac cgaccggacg gtgacgccga cgcccgtgaa ctccctcggg gtgaagggcg
     2041 caggcgagac gggcacgatc gcggccacgc cggccgtggt gaacgccgtc gtcgatgcgc
     2101 tggcgtccgc cggcgtcgac cacatcgaga ccatgccgct gacggccgag cgtgtctgga
     2161 aaaccatcca ggccgcgagg agataagact gagcgcgttc attgcccgac cgtcgggcgc
     2221 ggaggcgccc cgacggcaaa tcgatagact gagcgggttc actgcccgac cgtcaggggc
     2281 gcggacgccc cgacggcgaa cataggagac caccaccatg taccccgccg cattcgagta
     2341 ccacgcaccg acgagcgtgc aggacgcgct gggtctgctg ggccggctca aggacgatgc
     2401 caagcttctg gccggcggcc acagtctcgt gcccatgatg aagctgcgcc tggcccagcc
     2461 caagcacatc atcgacctcc gcaaggtgcc cgggctcgcg ggcatcaagg aggagggcgg
     2521 cgcgatcgtg atcggcgcga tgacgaccca ctggcagatg gagtcgtcgc cgttgctgaa
     2581 acagaagtgc ccgatcctca gcgagacggc atccatcatc ggcgaccccg ccgtgcgtaa
     2641 cgtcggcacc atcggcggct cgctcgcgca cgcggacccg gcggccgatc agccggccac
     2701 gatgatcgct ctcggcgtgg agttcgtgtg ccagggaccg aagggccggc gcacggtgaa
     2761 ggtggacgac tggttccagg gcctcatgtc caccgccgtc ggcgaggagg aactcctggt
     2821 ggagatccgc gtgccggcgt ggcccgccgg caccggcggc gcgtatatga agttcccgca
     2881 cccggcctcg cgcttcgccg tcgtcggcgt cggagcggtc gtgacactcg atggcgaagg
     2941 caggtgcacg agggccgggg tcggtgtgac gggcgcgggc acgaaggccg tgcgcgccaa
     3001 gggcgtggag gccgggctcg tcggcaagaa gctcgacgcg gcagccatcg aggccgcggc
     3061 ggcgaaggcc cacgaggggg tggacgtgca ggccgacctc caggggtcgg tcgagtacaa
     3121 gtcgcacctc tgccgcgtct tcgccaagcg ggcgatcacc gaagccgcca agcgggctcg
     3181 cgcgtaagcc tcccaccgcc gtcgaggggc ggggcggggt ccggcgacga cgccgtctgg
     3241 cgcgcgtggc cgcgcgtcgc gtgctcctac gtcgctgcat gcggcgtcat gcttgcggag
     3301 aagcggtcgg cggaccccgc actatgcgcg ccgtcttccc gccgccccac ccgccatctc
     3361 gtgagctccc gtcgacggct cgcccacgcc ggtgggactg cattcgatgg actccctgcg
     3421 gtcgtgacgt cggcgggcat cgccctgggt gtcgcgtggc tgccggaggt ggcgacgtga
     3481 tcgccgggat cgtgctggcg gccgggttcg ctcggaggat gggacgtcag aagctcctgc
     3541 tcgcgtaccg gggggcgccg atcgtgcggc tcagcgtcga ggccctggcg cggcacgtgg
     3601 aggacgtggt cgtcgtcacc gggcaggacg agggggcagt gcgcgaggcg ctggccgggc
     3661 tcgatgtccg cttcgcgcgc aacccgcggc cgcacgacgg gctaggatcg tcgatcgcgg
     3721 tgggcgtggc cgcgctcaag ccgtggacga ccgccacgct cgtcgccctg ggcgatcagc
     3781 cgcacgtacc cgccccggtg gtcccggcgc tgctcgccac gttcgggcgc gggggcaagg
     3841 ccatcgtggc acccgtgtac cggggtacgc aggggacacc agtgctcttc ggcagcgagg
     3901 tgttcgcgga actatcggcg ttgacgggag acagcggggc ccgcgcggtc gtcgcttcgc
     3961 gccccgatcg cgtcgcgctc gttccgttcg atctgccgat gccggccgac gtggatacgc
     4021 ccgaggactt cgcgcggctt catgtagaat agacaggcgt gcgtcccgag atcctgagaa
     4081 tccagcaggc gatggagggg gcggagtacg tcaccgacgc ccccgtcgcc acctcggtgc
     4141 acctcgcgct cgcgctcccc aagccgctcc tcatcgaagg tcacgcgggt gtgggcaaga
     4201 ccgaagtggc caaggtcatg gcgcgcgtgc tcggcacgaa cctgatccgg ctgcagtgct
     4261 acgaggggct cgacgtctcg accgcgctct acgagtggaa ctaccagaa
//
LOCUS       DATIAZ010000326         4178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1014818, whole genome shotgun sequence.
ACCESSION   DATIAZ010000326 DATIAZ010000000
VERSION     DATIAZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014818"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3048
                     /locus_tag="VLF19_11285"
     CDS             <1..3048
                     /locus_tag="VLF19_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSE93879.1"
                     /translation="YTPAHLAARILRERGALTGERKQVTVLFADVAGFSSLSERLDPE
                     EVHTLINRAFELTLAEVHAYEGTVNQFLGDGLMALFGAPVAHEDHAQRAAHAALGMQR
                     VLLRYREELQRERGIDFRVRLGLNTGLVVVGAIGDNLRMDYTAVGDTTNIAARMQQVA
                     EPGQILVAEPTQRLIAPYFELRQLGTFPVKNRAQPVAAWELCQPRARVSRLAARAAHG
                     LSPFVGRDEALAELERAWAAARGGRGQVALVVGDAGMGKSRLLFELQRRIGEAATWVQ
                     GDCVSYGQSIPFLPVVDMLKRNFGIEDEDGEAAVIARIGRGCAFLGADAPSVEPFLRY
                     LLSVDPGDPAIATMDSAQRRAQVVAALHRLTAAGSRRRPIVLVVEDAHWIDGATEDYL
                     KSLVDGIPGLGALLIVTYRPVYQQPFGDRTYFWRIALHPVEEDAALRIVRAALAVDDL
                     PADLVAAISGKAEGNPFFLEELGRALMETGAVRSVSGRLVLAQPANTLVVPETVQDLI
                     AARLDRLAEPQKRTVQTAAVIGREFALSLLRRVSDVQGQLEQSLDELKRIELIYERLG
                     AHDLEYVFRHALTQDVAYGSMLQSERRRLHALIGAAIEDVHAERLEERTEELVYHFTR
                     GEVWDKVVPYAREAAERAAALCVDDRAVEFYETALQALRHLPESPDTARAGVEVRLAM
                     RAPLWRGGEPERLFALFKEAEPLASRYGLTEHLDTIYAFLVQYHWARGEHDRAIDYGQ
                     RCLERAAARDDLGLRVTGMYYLCHAYWAIGRFSDALREARDIMALLAGPRATDRFGLS
                     GMPYAGACGFAAMALAELGDFTGALEMLDAGQRVADGADHLYSQMVVAATRGRVLVAT
                     GRAHDSIVLLEQTAATCRDKRFVGQLINALKYLAEAYVMTGRPADAIAPARESIALQE
                     QAKVYVERPFKHSTLARAYLALGDLDQAEAELGQALAFAERNGERGNEGWARLVGAEI
                     AARRGDRACAARLLDEAQDVAEELAMRPLLERCRALLRSIAAA"
     gene            complement(3416..>4178)
                     /locus_tag="VLF19_11290"
     CDS             complement(3416..>4178)
                     /locus_tag="VLF19_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE93880.1"
                     /translation="RDSRLQLIPSKHASIFWIEFPEQWDPKSPWHDKRLRLAVNHALD
                     RKAISESACLGFCPPAGVIVPRVLDFALQATPVAYDPNKAKQLLAEAGYPQGFDAGDF
                     TPIPPFAAGEAMANYLNAAGIRVRIRPMERAAFYSAWREKKMKGLFFTAAGNSGNAAT
                     RVEAFMYSKGTYAYGGYPDIDDMFLQQANERDTAKREAILHRIQQLTIDRVMFAPVMD
                     FRTLRGVGPRLAEHALDVMPLVPYPLYEDMKLKGQ"
BASE COUNT          576 a         1534 c         1462 g          606 t
ORIGIN      
        1 tacacccccg cgcacctggc cgcgcgcatc ctgcgcgagc ggggcgcgct caccggcgag
       61 cgcaagcagg tcacggtgct ctttgccgac gtggcgggat tcagctcgct gtccgagcgg
      121 ctcgatcccg aggaggtcca caccctcatc aaccgcgcct tcgagctgac gctggccgag
      181 gtccacgcct acgagggcac cgtcaatcag ttcctcggcg acggcctcat ggcgctgttc
      241 ggcgcgccgg tcgcccacga ggatcacgcg cagcgcgctg cccacgccgc gctgggcatg
      301 cagcgcgtgc tcctgcgcta ccgcgaggag ctgcagcgtg agcgaggcat cgacttccgc
      361 gtccggctcg ggctgaacac tggcctcgtc gtcgtcgggg ccatcggcga caacctgcgc
      421 atggattaca cggcggtcgg cgacacgacc aacatcgccg cccgcatgca gcaggtggcc
      481 gagcccggcc agatcctggt cgccgagccc acgcagcgcc tcatcgcgcc ctacttcgag
      541 ctccggcagc tcggcacgtt ccccgtgaag aaccgcgcgc agccggtcgc cgcctgggag
      601 ctgtgccagc cgcgcgcccg tgtctcgcgc ctggccgctc gagccgccca cgggctgtcg
      661 ccgttcgtcg gccgcgacga ggcgctggcc gagctggagc gcgcgtgggc cgccgcccgc
      721 ggcggccggg gccaggtcgc cctcgtcgtc ggcgacgccg ggatgggcaa gtcgcgtctg
      781 ctcttcgagc tgcagcgacg gatcggagag gccgccacct gggtccaggg ggactgcgtc
      841 tcgtacggcc aatcgatccc gttcctgccc gtggtggaca tgctcaagcg caacttcggc
      901 atcgaggacg aggacggtga ggccgccgtg atcgccagga tcggtcgcgg ctgcgccttc
      961 ctcggcgccg acgcgccgag cgtcgagccg ttcctgcgct acctgctctc cgtcgacccc
     1021 ggcgacccgg cgatcgcgac gatggactcg gcgcagcggc gggcccaggt cgtggcggcg
     1081 ctccaccggc tgaccgccgc cggcagccgg cgccggccca tcgtgctcgt cgtcgaggac
     1141 gcccactgga tcgacggcgc caccgaggac tacctcaagt ccctggtgga cggcatcccg
     1201 gggctgggcg cgctcctgat cgtcacctac cgtcccgtct accagcagcc cttcggcgat
     1261 cgcacgtact tctggcggat cgctctgcac ccggtcgaag aggacgccgc gctgcgcatc
     1321 gtgcgcgccg ccctcgcggt ggacgacctg cccgccgatc tggtcgccgc catctccggc
     1381 aaggcggaag gcaatccgtt cttcctcgag gagctgggcc gcgcactgat ggagacgggc
     1441 gctgtccggt ccgtgagcgg ccgcctggtc ctggctcagc cggcgaacac gctcgtggtc
     1501 ccggagaccg tccaggacct catcgcggcc cggctggatc gcctggccga gccccagaag
     1561 cgcaccgtgc agaccgccgc cgtcatcgga cgcgagttcg cgctctcgct gctgcggcgc
     1621 gtatcggacg tgcagggcca gctcgagcag agcctggacg agctcaagcg gatcgagctc
     1681 atctacgagc ggctcggcgc ccacgacctc gagtacgtct tccgccacgc cctcacccag
     1741 gacgtcgcct acgggtccat gctgcagtcc gagcgccggc ggctgcacgc tctcatcggc
     1801 gccgccatcg aggacgtcca cgccgaacgg ctcgaggagc gcacggagga gctggtctac
     1861 cacttcaccc gcggcgaggt gtgggacaag gtcgtgccct acgcgcgcga ggccgccgag
     1921 cgggccgccg cgctctgcgt ggacgaccgt gcggtggagt tctacgagac ggcgctgcag
     1981 gcgctgcgcc acctgccaga gtctccggac accgcccggg ccggtgtcga ggtgcgactg
     2041 gcgatgcgcg cgccactgtg gcggggcggt gagcccgagc gcctcttcgc cctcttcaag
     2101 gaggccgagc ccctcgccag ccggtacggc ctcaccgagc acctcgacac gatctacgcg
     2161 ttcctcgtgc agtatcactg ggcgcggggc gagcatgacc gggcgatcga ctacggccag
     2221 cgctgcctcg agcgggccgc cgcccgcgac gacctcggcc tgcgcgtgac cggcatgtac
     2281 tacctctgcc acgcgtactg ggccatcggc cgattctccg acgcgcttcg cgaggcgcgc
     2341 gacatcatgg cgctgctggc cgggccgcgc gccaccgacc gcttcggcct ctcgggcatg
     2401 ccctacgccg gcgcgtgcgg gttcgccgcc atggcgctgg ccgagctggg cgacttcacc
     2461 ggcgcgctgg agatgctgga cgcgggccag cgcgtggcgg acggcgccga tcatctgtac
     2521 tcccagatgg tcgtggccgc gacacggggc cgggtgctcg tcgcgacggg ccgcgcccac
     2581 gactcgatcg tgctgctgga acagacggcg gcgacctgcc gggacaagcg gttcgtcggc
     2641 cagctcatca acgccctgaa gtacctggcc gaagcctatg tcatgaccgg ccgtcctgcc
     2701 gacgccatcg cgcccgcccg ggagtccatc gcgctgcagg agcaggcgaa ggtgtacgtc
     2761 gagcgcccct tcaagcactc caccctggcc cgcgcctacc tggcgctcgg cgacctcgac
     2821 caggccgagg ccgagctggg ccaggcgctc gcgttcgccg agcgcaacgg cgagcggggg
     2881 aacgaaggct gggcgcgcct ggtgggagcg gagatcgccg cccgccgggg cgatcgcgcc
     2941 tgcgccgccc gcctcctcga cgaggcgcag gacgtcgccg aggagttggc gatgcgcccc
     3001 ctgctcgagc gctgccgcgc gctcctgcgg tccatcgccg cggcatagcc ggcccaggct
     3061 cgcgcccgag gagccgtcga cctcggcaac ctttctcgcc cggcggagtc tcacgaccat
     3121 cgtgttctcg acccaccgtc ccgaggaggc ggcggcggtc tgtgacgaga tcttgatcct
     3181 gggacgggga caggcggtgg cgtcagggag cccgggaaca tcacgagaca ggcggccggg
     3241 caggtcttcg agacatcgct ccgccggccg catctccccg cggacaagac ctgggacgtc
     3301 gtatcggccg agcgcgacgg cgagcacgtc caccttcgcg tggtgagcgc ggcgcccccc
     3361 gacgcccggc cggtgccgcc gcgcctcacc gacgcttacg tgctcctgac gtccgctact
     3421 ggcccttgag cttcatgtcc tcgtagagcg gatacggcac cagcggcatc acgtcgagcg
     3481 cgtgctcggc gagccgcggg ccgacccctc tcagcgtccg gaaatccatg accggcgcga
     3541 acatcacccg gtcgatcgtg agctgctgga tgcgatgcag gatggcctcg cgcttggccg
     3601 tgtcccgctc gttggcctgc tgcaggaaca tgtcgtcgat gtcgggatag ccgccgtagg
     3661 cataggtgcc cttggagtac atgaaggcct ccacgcgggt ggcggcgttg ccggagttgc
     3721 cggccgcggt gaagaacagc cccttcatct tcttctcgcg ccaggcggaa tagaaggccg
     3781 cgcgctccat cggcctgatc ctcacgcgga tgccggcagc gttgaggtag ttcgccatgg
     3841 cctcccccgc ggcgaagggc gggatcggcg tgaagtcgcc ggcgtcgaag ccctgcggat
     3901 agccggcctc ggcgagcagc tgcttggcct tgttcgggtc gtaggccacg ggcgtcgcct
     3961 ggagcgcgaa gtccaggacg cgcggcacga tgaccccggc cggcgggcag aagccgagac
     4021 aggcgctctc gctgatcgcc ttgcgatcga gcgcgtggtt gacggccagc cgcaggcgct
     4081 tgtcgtgcca cggcgacttc ggatcccact gctcggggaa ctcgatccag aagatcgacg
     4141 cgtgcttcga cgggatcagc tgcaggcgcg agtcccgc
//
LOCUS       DATIAZ010000327         6301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_886068, whole genome shotgun sequence.
ACCESSION   DATIAZ010000327 DATIAZ010000000
VERSION     DATIAZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886068"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VLF19_11295"
     CDS             complement(<1..969)
                     /locus_tag="VLF19_11295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSE93881.1"
                     /translation="MLAERMKTLAPSSTLAVQARAKELRARGVDVISFGAGEPDFDTP
                     ERIKQAAIQAMRRGQTKYTEVGGILELRAAVCTKFKRDNGLDYEPADVLVSVGAKHTL
                     YNLAVALLDPGDEVIVPSPFWVSYPEQARLVGAVPVAVETHERAGFDLDPDRVRAAIT
                     PRTKLIVVNSPNNPTGAVFSGEALEAVARLAIERGLWVASDECYESLTFEGRHVSIAK
                     LGPEIKARTIVVNTCSKAYAMTGWRIGFAGGPRALIRAMTDVQSQVTSNPSSVAQWAA
                     VEALAGPQDEVAVMAAEFDRRRHLVVEGLNALPGVRCVMPKGAFYAF"
     gene            complement(989..1486)
                     /gene="coaD"
                     /locus_tag="VLF19_11300"
     CDS             complement(989..1486)
                     /gene="coaD"
                     /locus_tag="VLF19_11300"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSE93882.1"
                     /translation="MAKRAVYPGMFDPIHNGHLDLIRRSLQIFDELIVAVVANPSKEP
                     LFPVALRLEMIDEATADLKSNFRIVAFDGLLIDLVARERADCIVRGIRAVSDFEYEFQ
                     MALMNRKLSSRVETVFLMPHERYTYISSRLIKEVASYGASVTGLVPPVAEKRLAEKYP
                     PKSPA"
     gene            complement(1535..2143)
                     /gene="rsmD"
                     /locus_tag="VLF19_11305"
     CDS             complement(1535..2143)
                     /gene="rsmD"
                     /locus_tag="VLF19_11305"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSE93883.1"
                     /translation="MRILAGEWKGRRLRTPRGETTRPTADQVRIALMDTLMPWLPGAR
                     VLDLFAGAGGVGLEALSRGAAHTTFVERDGRAVAALRANVAALGVEARVRIVRDAAAR
                     ALGRLAREGARFDVVFLDPPYDSTLAADTLARLGEGGVTREGAIVVAQHLTKRAPAER
                     VGTLGAWRVRRFGETTLTFFRAGGYDAVSRIPESESSCGSEP"
     gene            complement(2145..4235)
                     /gene="recG"
                     /locus_tag="VLF19_11310"
     CDS             complement(2145..4235)
                     /gene="recG"
                     /locus_tag="VLF19_11310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSE93884.1"
                     /translation="MRQTGGRAGLDTALQYLPGVGPQRAALLAKKGLTTVRDALFFVP
                     LRHEDRTRLTPLRALIPGQTVTCTGLIAGLSPPPPGRPQVPFTVMLRDETGHVAASWF
                     RAPFLSRVFERGQRLVLHGKVTRFRGVLTLQHPDYEIVESGEDDRLHTGRLTPVYSTT
                     EGLPQRGLRRLMSAIVEQFTGEVPEALPEAVRVRRRLVPLATALRDAHFPQTDEASAA
                     ARRRMVFDEFLFLQLGLAILRSRTTRARGIAMSPRGDLTRRLRDTLPYRLTAAQERVW
                     DEIRRDMAAPFPMHRLLQGDVGSGKTVVAAQAVLTAIEAGYQAAVMAPTEILAEQHYM
                     TFRQLLHPLGVRVTLLTSALKVRERTQRRAAVAAGEVDCAIGTHALVQEDVAFRRLGL
                     AVVDEQHRFGVAQRARLKAKGEHPDMLVMTATPIPRTLALTLYGDLDVSVLDELPPGR
                     RPVKTVARAGAKRREIYAFIRDQVAAGRQAYVVYPLVEESEVVDLKAATDMARHLAQD
                     VFPDLAVGLLHGRMAFDEKDAIMRRFKDGQLHVLVCTTVIEVGIDVPNASVMLVEHAE
                     RFGLSQLHQLRGRVGRGPWTSYCILLHGADLTEEARRRIEALVASSDGFRIAETDLEI
                     RGPGEFFGTRQSGLPEFRVADLVRDTALLEEARAEALRIVAADAELRDPAHRALRDGL
                     LARWRGKLALASVG"
     gene            4300..4491
                     /gene="rpmB"
                     /locus_tag="VLF19_11315"
     CDS             4300..4491
                     /gene="rpmB"
                     /locus_tag="VLF19_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSE93885.1"
                     /translation="MAQRCDVCGKGPSVGHTISHAHKLTRRRWLPNLVSMRALVRGKA
                     QRVRVCTRCLKAGKVTKVI"
     gene            4544..4744
                     /locus_tag="VLF19_11320"
     CDS             4544..4744
                     /locus_tag="VLF19_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93886.1"
                     /translation="MGGRHPPPLKRAYFFFFLAAFFFFAMYPLTSSLRNSAPGQEGPR
                     YFFFFLAAFFFAMDPFTPLRPR"
     gene            complement(4741..5790)
                     /locus_tag="VLF19_11325"
     CDS             complement(4741..5790)
                     /locus_tag="VLF19_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93887.1"
                     /translation="MLKSEEIRRQAGQRLREVFAKARAWDESADVRIGNQKADLVKFR
                     LGDQEHTLVLEVCSLGQPRQIRAAVTRLGEIRREMPNAYPLAVAVYIGPQSARILKNN
                     NLGYLDLSGNCYVALENVLIEKEGKRNVRPSTRPLRSLFAPRATRVVRVLLAEPARVF
                     KLEELAKAATVSLGHAHNVVKRLEELAWVERDDRQRIRLTKPADLLENWGESYTYRDN
                     EIFSYFAPERVSRKFTTDVARAAEATGRRYAFTLNAGASLVVAAHLRVPAVHCYLEGD
                     PAPVAAALGLRPATEAEGSLHLLGPYDPGVFHGALEKGGLKVTCLPQLYVDLLHYERK
                     GAEQAEHLRREAMGF"
     gene            complement(5963..>6301)
                     /locus_tag="VLF19_11330"
     CDS             complement(5963..>6301)
                     /locus_tag="VLF19_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HSE93888.1"
                     /translation="ALRRLPRAFKEKLANVAVVVEDWADDETLADVGVEPPDTLYGLY
                     QGVDITRRDSSYGNVLPDTITIYQGPIEEDCADEAEMADLVRETVVHEIGHYFGLDDD
                     TMHRIEEGEE"
BASE COUNT          911 a         2244 c         2187 g          959 t
ORIGIN      
        1 gaatgcgtag aacgccccct tcggcatcac gcaccgcacg ccgggcagcg cgttgaggcc
       61 ttcgacgacg agatgccgcc gccggtcgaa ctcggcagcc atcacggcca cctcgtcttg
      121 gggtcccgcc agcgcctcca cggcggccca ctgcgccacc gaggacgggt tggaggtgac
      181 ctggctctgc acgtccgtca tggcgcggat cagcgcgcgc ggaccaccag cgaagccgat
      241 ccgccagccg gtcatcgcgt aggccttgga acacgtgttg accacgatcg tccgcgcctt
      301 gatctcgggc ccgagcttcg cgatcgagac gtggcgtccc tcgaacgtga gcgactcgta
      361 gcactcgtcg gaggcgaccc acagcccgcg ctcgatggcc agccgcgcga cggcctccag
      421 cgcctcgccg gagaacacgg cgccggtcgg gttattcggg ctgttgacca cgatcagctt
      481 cgtccgcggg gtgatggccg cgcgcacccg gtcggggtcg agatcgaagc ccgcccgctc
      541 gtgggtctcg acggcaacgg ggacggcgcc gaccaggcgc gcctgctcgg gatacgagac
      601 ccagaacggg ctgggcacga tcacttcgtc gccggggtcg agcagcgcga cggccaggtt
      661 gtagagcgtg tgcttggccc ccacggagac gagcacgtcg gccggctcgt agtcgaggcc
      721 gttgtcgcgc ttgaacttcg tgcagacggc cgcccgtagc tcgaggatgc cgcccacctc
      781 cgtgtacttg gtctgccccc gccgcatcgc ctggatggcg gcctgcttga tgcgctcggg
      841 ggtgtcgaag tccggctcgc cggcgccgaa ggagatcacg tcgacgcccc gtgcccgcag
      901 ttccttcgcc ctggcctgca cggccagggt cgacgacggg gcaagggtct tcatccgctc
      961 ggccagcacg ggcggctcct ccgccggctc aggccggcga cttcggtggg tacttctcgg
     1021 ccagccgctt ctcggcgacc ggcggcacca gcccggtcac cgaagctccg tagctggcga
     1081 cttccttgat cagccgcgaa gagatgtacg tgtaacgctc gtgcggcatg aggaacacgg
     1141 tctccacgcg gctcgagagc ttgcggttca tcagcgccat ctggaactcg tactcgaagt
     1201 ccgagacggc acggatgccg cgcacgatgc agtcggcgcg ctcgcgggcc accaggtcga
     1261 tgagcagccc gtcgaaggcc acgatgcgga agttggactt cagatctgcc gtggcctcgt
     1321 cgatcatctc caggcgcagg gccaccggga acagcggctc cttcgacggg ttggcgacca
     1381 cggcgacgat cagctcgtcg aagatctgca ggctgcggcg gatgaggtcg aggtgaccgt
     1441 tgtgaatcgg gtcgaacatg ccgggataga cggcgcgctt ggccacgtcg gaacctcgct
     1501 caggcttgcc gcgggcacgc cccccggctt cgcactacgg ttcggacccg cacgacgatt
     1561 cggactcggg gatgcgggac acggcatcgt acccgccggc tcgaaagaaa gtcaaggtcg
     1621 tctccccgaa gcgccgcacg cgccacgcgc cgagcgtgcc gacgcgctcg gccggcgcgc
     1681 gcttggtgag gtgctgggcc actacgatgg ccccctcacg cgtgacccca ccctcgccga
     1741 gccgggcgag cgtgtccgcc gccaacgtgc tgtcgtacgg cggatcgagg aacaccacgt
     1801 cgaagcgcgc cccctcgcgg gccaggcgcc ccagcgcgcg ggcggcggcg tcgcggacga
     1861 tccggacgcg cgcctcgacg ccgagcgcgg cgacgttggc gcggagcgcg gcgaccgcgc
     1921 gcccgtcgcg ctcgacgaag gtcgtgtggg ccgcgccgcg cgacagcgcc tcgaggccga
     1981 cgccgccggc gccggcgaag aggtcgagca cgcgcgcccc cggcaaccac ggcatgaggg
     2041 tgtccatcag cgcgatgcgc acctggtcgg ccgtcggccg cgtggtctcg ccgcgcggcg
     2101 tccggagccg ccggcccttc cactcccccg cgaggatgcg cacgtcaccc caccgacgcg
     2161 agggccagct tgccgcgcca gcgcgcgagc agtccgtcgc gcagcgcccg gtgtgccggg
     2221 tcgcgcagct cggcgtcggc ggcgacgatg cgcagcgcct cggcgcgtgc ctcttccagc
     2281 agcgcggtgt cgcggacgag gtcggcgacg cggaactcgg gcagccccga ctgccgcgtg
     2341 ccgaagaatt cccctggccc tcggatctcg aggtcggtct cggcgatacg gaagccgtcg
     2401 ctgctcgcca ccagcgcctc gatgcggcgc cgggcttcct cggtgagatc ggccccgtgc
     2461 aggaggatgc aataggacgt ccacggcccg cggccgacgc gcccgcgcag ctggtggagc
     2521 tgggacagcc cgaaccgctc ggcgtgctcc accagcatca cggaggcgtt cgggacatcg
     2581 atacccacct cgatcaccgt cgtgcagacg agcacgtgga gctggccgtc cttgaagcgc
     2641 cgcatgatgg cgtccttctc gtcgaaggcc atccgcccgt gcaggagccc gacggcgagg
     2701 tcgggaaaga cgtcctgggc gaggtggcgc gccatgtccg tggccgcctt gaggtccacc
     2761 acctccgact cctcgacgag cggatagacg acgtacgcct ggcgcccggc ggcgacctgg
     2821 tcgcggatga aggcgtagat ctcgcgtcgc ttcgctcccg cgcgcgccac cgtcttgacg
     2881 ggtcgccggc cgggcggcag ctcgtcgagc acgctgacgt cgaggtcgcc gtagagcgtg
     2941 agcgccagcg tccgggggat cggcgtcgcc gtcatgacga gcatgtcggg gtgctcgccc
     3001 ttggccttga ggcgcgcccg ctgcgcgacg ccgaagcggt gctgctcgtc caccacggcg
     3061 agcccgaggc gccggaacgc gacgtcctcc tgcacgaggg cgtgggtgcc gatcgcgcag
     3121 tccacctcgc cggcggccac cgcggcccgc cgctgggtgc gctcgcgcac cttcagcgcc
     3181 gaggtgagca gcgtcacgcg gacgcccagg ggatggagga gctgccggaa cgtcatgtag
     3241 tgctgctcgg cgaggatctc cgtcggcgcc atgaccgccg cctggtagcc ggcctcgatc
     3301 gccgtgagca cggcctgggc tgccacgacc gtcttgcccg agccgacgtc gccctggagg
     3361 agacggtgca tgggaaaggg cgcggccatg tcgcggcgaa tctcgtccca cacgcgctcc
     3421 tgcgcggccg tgagccggta gggcagcgtg tcgcgcagcc gtcgagtgag atcgccccgc
     3481 gggctcatgg cgatgccgcg cgcgcgcgtg gtgcgcgacc tcaggatggc cagcccgagt
     3541 tgcaggaaca ggaactcgtc gaagaccatc cgccgccggg cggcggcgct cgcctcgtcc
     3601 gtctgcggaa agtgggcatc gcgcagcgcc gtcgccagcg ggaccaggcg gcgccgaacc
     3661 cggacggcct ccggcagggc ctccggcacc tcgccggtga actgctcgac gatcgcggac
     3721 atgagccgcc gcaggccgcg ctgcggcaac ccctcggtgg tcgagtacac gggcgtgagc
     3781 cgtcccgtgt gcagccggtc gtcctcgccg gactcgacga tctcgtagtc ggggtgctgg
     3841 agcgtgagca cgccgcggaa gcgcgtgacc ttgccgtgga gcacgaggcg ctgcccccgc
     3901 tcgaacaccc gcgagagaaa tggcgcccga aaccagctcg ccgcgacgtg acccgtctcg
     3961 tcgcgcagca tcaccgtgaa cgggacctgg ggccgaccgg gcggcggcgg gctcaggccg
     4021 gcgatcagcc cggtgcacgt gaccgtctgc ccgggaatca gcgcgcgcag cggcgtgagg
     4081 cgcgtgcggt cctcgtggcg cagaggcacg aagaagaggg cgtcccggac ggtcgtgagc
     4141 cccttcttcg ccagcagcgc cgcgcgctgg ggaccgacgc ccgggaggta ctggagcgcc
     4201 gtgtcgagcc cggcgcgccc gccggtttgt ctcacccgag cggctgtgct ataaggggtc
     4261 ttcgtctcgg acggtcccat tcaggccgaa ggagcaagga tggctcagcg ctgcgacgtc
     4321 tgcgggaaag ggccctccgt gggtcatacg atcagccacg cgcacaagct cacgcgccgg
     4381 cgctggctgc ccaacctcgt ctcgatgcgg gccctcgtcc gcggcaaggc gcagcgcgtc
     4441 cgggtctgca cccgctgtct caaggcgggc aaggtcacga aggtcattta agcacagacg
     4501 gataacggga gggggcgcat gccccctccc ggttgatgac aaggtgggag gccgccaccc
     4561 cccacccctg aagcgggctt acttcttctt cttcttggcc gccttcttct tcttcgccat
     4621 gtaccctctc acctcctctc tccggaactc ggcacctggc caggagggcc cgcgctactt
     4681 cttctttttc ttggccgcct tcttcttcgc catggatccg ttcacccccc ttcgaccgcg
     4741 ctagaagccc atcgcctcgc ggcgcaggtg ctcggcctgc tccgcgccct tgcgctcgta
     4801 gtgcaacagg tcgacgtaga gctgaggcag acacgtcacc ttcagcccgc ccttctcgag
     4861 ggcaccgtgg aagacgcccg ggtcgtaggg ccccagcagg tgcaggctgc cctcggcctc
     4921 cgtcgccggc cggagcccga gcgccgcggc cacgggcgcc gggtcgccct cgaggtagca
     4981 gtgcacggcg ggaacgcgga ggtgcgccgc gacgaccagc gaggcgcccg cgttcagcgt
     5041 gaacgcgtat cgccggccgg tggcctcggc ggcacgggcg acgtcggtcg tgaacttgcg
     5101 cgagacgcgc tcgggtgcga agtaggagaa gatctcgttg tcgcggtagg tgtatgactc
     5161 gccccagttc tcgagcagat cggccggctt ggtgagccgt atgcgctgcc ggtcgtcccg
     5221 ctcgacccac gccagctctt ccaggcgctt gaccacgttg tgggcgtgtc cgaggctcac
     5281 ggtggcggcc ttcgccagct cctccagctt gaagacgcgc gcgggctccg ccagcagcac
     5341 ccgcaccacg cgcgtggcgc gcggcgcgaa cagcgatcgc agcgggcgtg tggagggccg
     5401 gacgttgcgc ttgccttcct tctcgatgag cacgttctcg agggcgacgt agcagttgcc
     5461 cgagaggtcg aggtagccga ggttgttgtt cttgaggatc cgggcactct gcggcccgat
     5521 gtacacagcc acggccagcg gatatgcgtt cggcatctcc cgccggatct cgcctagccg
     5581 cgtgaccgcc gcgcggatct ggcggggctg cccgagcgag cacacctcaa gcaccagcgt
     5641 atgctcctgg tctccgagac ggaacttcac caagtccgcc ttttgattgc cgatccggac
     5701 gtcggcgctc tcgtcccagg ccctggcctt ggcgaacacc tcgcgcaggc gctggccggc
     5761 ctgtcgccgg atctcttcgg atttgagcat gtttcgcttg ctccaagcct ttcagcatcc
     5821 gctgaaacat tacgaacgac tgaaagtccc tgtcaagcag aaacttacgg tcttgctcta
     5881 gaacaccttg gtggtaggac ctgcctggac tggatacagt cggctcaacg ggctgcgcca
     5941 cggctccgcc gggcggtggg agctactcct cgccttcctc gatgcggtgc atggtgtcgt
     6001 cgtcgaggcc gaagtagtgg ccgatctcgt ggaccaccgt ctcgcgcacc agatccgcca
     6061 tctcggcctc gtcggcgcag tcctcctcga tcgggccctg gtagatcgtg atcgtgtcgg
     6121 gcaagacgtt gccgtacgag gaatcgcggc gcgtgatgtc cacgccctgg tacaggccgt
     6181 acagcgtgtc gggcggctcg acgcccacgt cggccagcgt ctcgtcgtcc gcccagtcct
     6241 cgacgaccac cgcgacgttc gccagcttct ccttgaacgc ccgcggcagc ctccgcaacg
     6301 c
//
LOCUS       DATIAZ010000328         9731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1806359, whole genome shotgun sequence.
ACCESSION   DATIAZ010000328 DATIAZ010000000
VERSION     DATIAZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806359"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="hisH"
                     /locus_tag="VLF19_11335"
     CDS             complement(<1..207)
                     /gene="hisH"
                     /locus_tag="VLF19_11335"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076131.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSE93889.1"
                     /translation="MIAVVDYGRGNLGSVEKAFERVGTPAVVTRDPRAVDEADAVVLP
                     GDGAFHDAMSNLAGLGLMDAMRRAL"
     gene            complement(290..895)
                     /gene="hisB"
                     /locus_tag="VLF19_11340"
     CDS             complement(290..895)
                     /gene="hisB"
                     /locus_tag="VLF19_11340"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSE93890.1"
                     /translation="MSGDRVSRQARVERKTKETEVAVILNVDGTGASKVQTPIPFFSH
                     MLEAWAKHGLMDLGVEATGDVEVDIHHTVEDVGIVLGQALRQALGDRRGIVRFGTVFV
                     PMDEALVASSVDISGRPFLVFNVPVARTRVSNFDLDMLQEFFRALAFNAEITLHVTMH
                     YGHNLHHISEAVFKAVGRALAAATRIDPRIAGILPSTKGTL"
     gene            complement(892..2199)
                     /gene="hisD"
                     /locus_tag="VLF19_11345"
     CDS             complement(892..2199)
                     /gene="hisD"
                     /locus_tag="VLF19_11345"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSE93891.1"
                     /translation="MIRVLDLKTLGLDAVVAALERTPGGATPEQHRTVETILRAVRER
                     GDDALLFTTEKYDGFKARSAADLRIAPAEFEAAERALAPATRAALAYAAERIERYHAA
                     ALPKSWRITDEHGSVLGQEVRPLERVGVYVPGGRAAYPSSVLMTAVPARVAGVPEIVL
                     VTPPASDGRVNPTVLAAARIAGVTEGYRVGGAQAVAALACGTPTIRRVDKIVGPGNVY
                     VTLAKSRVFGEVGIDMVAGPSEVVVVADGTADPVWIAADLLAQAEHDPMARAVLVTDA
                     APLIDAVAAALADRLVRLPRRAIAADALRDHGALVLAPTLDEAVELANRLAPEHLELY
                     VSAPRALLARVRHAGAVFVGGLTPEVVGDYVAGPSHVLPTAGTARFSSPLGVEDFVKR
                     TSVIEYSTAGLRAALPHLSVLAEVEGLHGHRAAAGVRTEGGSR"
     gene            complement(2196..2843)
                     /gene="hisG"
                     /locus_tag="VLF19_11350"
     CDS             complement(2196..2843)
                     /gene="hisG"
                     /locus_tag="VLF19_11350"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSE93892.1"
                     /translation="MNERLTLALPKGRLLEGALGLLRALGVDGVDAASRKLIHVDPVR
                     ELRLLFLKPADVPAYVTYGAADLGIVGKDILLEQEPDVYEPLDLGFGFCRLVVAEPRE
                     LWERDDPSKWSWVRVATKYPRMAERYFSERGVQVEIVRLDGSIELAPLVGLAERIVDL
                     VQSGETLRVNGLVEVAEIARSTARLIVNRASMKTEYRAVTELIEEMRARPAGVSR"
     gene            complement(2840..4093)
                     /gene="murA"
                     /locus_tag="VLF19_11355"
     CDS             complement(2840..4093)
                     /gene="murA"
                     /locus_tag="VLF19_11355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSE93893.1"
                     /translation="MPARLLIEGGVPLRGTVAVSAAKNAALPAMAAALLTSEPVTLTN
                     VPDLVDVKTMGRLLETLGARLERDGQRATLRVGRVSSDVAPYALVSTMRASVLVLGPL
                     VARHGSARVALPGGCTIGVRPIDQHLKGLAKLGAEIAIESGYVVARASRLKAARIATD
                     LVTVTGTENLLMAAALAQGATVIENAACEPEVVDLARLLTAMGARIHGAGTSRIEIEG
                     VSDLGGATHTIVPDRIEAGTLIVAGAITRGDVTVTGLVSEHLTAVLAKLEECGVELTV
                     GADRVRARGPERARPSDVTTSPFPGFPTDMQAQLMTLLGIAGGQSRVTETIFENRFMH
                     AAELNRMGASIETEGSTAIIRGVPAYQGAPVMASDLRASAALVLAGLAARGRTEIARV
                     YHLDRGYERMEGKLAALGACIERRG"
     gene            complement(4075..5040)
                     /gene="prmC"
                     /locus_tag="VLF19_11360"
     CDS             complement(4075..5040)
                     /gene="prmC"
                     /locus_tag="VLF19_11360"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSE93894.1"
                     /translation="MSPAVAVPSMRAALAAATARLAGAGIDTPRVDAEWLLAGILGVG
                     RAALGARLAAGLTPAVAGRFEAAVGRRARREPLQHILGWEDFCGLRLAVTPDVLVPRP
                     ETETLVERALALLPPAGAIARRVIDVGTGAGGIACALAAARRDVRVVAVDLSEAASRL
                     ARRNVRAVGVADRVFVVVGDLFAGLGPTRSDLIVSNPPYLPSAMLAGLAPEVAKHDPR
                     LALDGGADGMHLIRRLVSEAPARLAPGGALALETAGGDQAPDVAALMREAGFVDVTLH
                     LDLTGAQRFVTGVNARPGKPPPPLLGGPAPERAVEAPSNVCPLGS"
     gene            complement(5037..6107)
                     /gene="prfA"
                     /locus_tag="VLF19_11365"
     CDS             complement(5037..6107)
                     /gene="prfA"
                     /locus_tag="VLF19_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSE93895.1"
                     /translation="MFDKLRQIEERSEELARALADPKIYGNQAEFARLRKEHAETQEI
                     VERFREYREVLKRLGEARHILEEGGDRELLELAQAESDELTARQAALETALKRLLVPR
                     DPNDDKNVFVEIRGGAGGDEAALFAADLARMYTKYAERQRWKVEVMDASPTGVGGLKD
                     VILFIQGRGAWSRLKFERGVHRVQRVPVTESAGRIHTSTVTVAVLPEAEDVEVKVDER
                     DLRVDVYRSSGPGGQGVNTTDSAVRITHLPTGLVVTCQDERSQIKNRAKAMRVLKARL
                     LERAQEEQQAAIAADRRSQVGTGERSERIRTYNFPQTRVTDHRIGLTLHRLPAVLEGD
                     LDELIDALAAAEQAERLEKVAQ"
     gene            complement(6149..6364)
                     /gene="rpmE"
                     /locus_tag="VLF19_11370"
     CDS             complement(6149..6364)
                     /gene="rpmE"
                     /locus_tag="VLF19_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HSE93896.1"
                     /translation="MKAGIHPEYVDTTISCACGEIIKTRSTRPEIRVEVCSKCHPFYT
                     GKQKLMDTAGRVERFQRKYKRQTTTPS"
     gene            complement(6462..8648)
                     /locus_tag="VLF19_11375"
     CDS             complement(6462..8648)
                     /locus_tag="VLF19_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSE93897.1"
                     /translation="MRVARLLNERLEVVGVCQWIVEHVLTLLPIRSSAITLLDPDGNL
                     HVLARDGDPLTASEGLVLPRGVGVTGRAVQESRPLWSGDVLGDTRFVWPGGIRDRLVA
                     GGQRALLAVPLRPRGRTIGALTVADRRPRAFTDAEVRLVQAFADQAAIALDNAQLHED
                     AERRRSEAEQLAQVARTMAGALGVSAVATHVAESLVAMFDALATCVARLDSDGALVML
                     AAHDAVGSLASADERVRPGRELLETAMREGRPCRSPDILDDSRIVIDEERRRFITGTQ
                     LRSVLAVPLVAGGHTIGALSLGFEAGRSVSERDTARLQAFADLAASALDRALLHDEAA
                     RAEAALRASEAELRQAQKMEAVGRLAGGIAHDFNNLVTIITGRSEFLLQRLAFDAAAC
                     RDLELIKTTAERAGTLTKQLLAFSRRQVLRPRVLDLNRVVAGVAQMLQRLIGEDVHLV
                     TRLAPDLGAVHADPTQIEQIILNLAVNARDAMPRGGRLRIETANVHLDDDFVAAHPDS
                     SRGAHVLLQVSDTGSGMSPEVQAHIFEPFFTTKEVGRGTGLGLATVYGIVKQHGGSIG
                     VESAPDVGATFRIYLQRVDDAGEPGGPDDEATASPPAPRTVLLVEDEDLVRELAHQVL
                     TSYGYETLAASGPAEALDLVRRHDGPIHLLLTDVVMPRMGGRALAECLQAERPEMRVL
                     YMSGYAEDEIVHRGGLAPGTPLLPKPFTLDAFMRKVRETLDLEPEG"
     gene            complement(9085..9729)
                     /locus_tag="VLF19_11380"
     CDS             complement(9085..9729)
                     /locus_tag="VLF19_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSE93898.1"
                     /translation="MRVVLADDHALVRAGIRSLLSAIGGVEVVAEARDGHEVVRLVET
                     LHPRLVLMDIAMPGLNGLEATARIVKAHPRTAVIILSMHAAEEYALQALRAGAAGYLL
                     KDADLAELELAISAVARGETYLSPAMSKHLIADYRRRVADQPDPLERLTSRQREVLQL
                     VAEGLTTKDIAAKLKLSVKTIETHRAQLMDRLDIRDVAGLVRFAIRVGLITPES"
BASE COUNT         1333 a         3560 c         3487 g         1351 t
ORIGIN      
        1 gagcgcgcgg cgcatggcgt ccatcaggcc caggccggcc agattcgaca tcgcgtcgtg
       61 gaaggccccg tcgccgggca gcacgacggc gtcggcctcg tccaccgcgc gggggtcccg
      121 cgtgaccacg gccggcgtgc ccacgcgctc gaaggccttc tccaccgagc cgaggttccc
      181 gcggccgtag tcgacgaccg cgatcacagg ctggcctcga tcctcacagg gtcgcctcgc
      241 ctccggctgc ggctcctgct ccggcctgcc gctcgcacgg ccacgtcgat cacagcgtcc
      301 ccttcgtgga cggcaggatt cccgcgatac gcgggtcgat gcgcgtggcc gcggccagcg
      361 cgcgtcccac cgccttgaac accgcctcgc tgatgtggtg caggttgtgc ccgtagtgca
      421 tggtcacgtg cagcgtgatc tcggcgttga aagcgagggc ccggaagaac tcctggagca
      481 tgtcgaggtc gaagttcgac acgcgcgtgc gggccacggg cacgttgaac accaggaacg
      541 gccggcccga gatgtccacc gacgacgcga cgagcgcctc gtccatcggc acgaagacgg
      601 ttccgaagcg cacgatgccg cgccggtcgc cgagcgcctg gcgcagggcc tgcccgagca
      661 cgatgccgac gtcctcgacg gtgtggtgga tgtccacctc gacgtccccg gtggcctcga
      721 cgccgaggtc catgaggccg tgcttcgccc acgcctccag catgtggctg aagaacggga
      781 tcggcgtctg caccttcgac gcacccgtgc catccacgtt gaggatgacg gcgacctccg
      841 tctccttggt cttccgctcg acccgcgcct gccgggacac ccggtctccg ctcatcgcga
      901 tcccccctcg gtgcggaccc cggcggccgc ccggtggccg tggagcccct ccacctccgc
      961 cagcacgctc agatgcggca gcgccgcgcg gaggccggcc gtcgagtact cgatgacgct
     1021 cgtccgcttc acgaagtcct cgacgccgag cggcgaggag aagcgcgcgg tgccggccgt
     1081 gggcagcacg tggctcggcc cggccacgta gtcgcccacc acctccggcg tcagccctcc
     1141 cacgaagacg gcgccggcgt ggcgcacgcg cgcgagcagc gcccggggag cgctgacgta
     1201 cagctccagg tgctccggag ccaggcggtt ggccagctcg accgcctcgt cgagcgtggg
     1261 ggccagcacg agagcgccgt ggtcccgcaa cgcgtcggcg gcgatggccc ggcgcggcag
     1321 ccgcacgagc cgatccgcga gggctgcggc cacggcgtcg atcagcgggg ctgcatccgt
     1381 gacgaggacg gcgcgcgcca tcgggtcgtg ctcggcctgg gcgagcaggt cggcggcgat
     1441 ccagaccgga tcggccgtcc catcggcgac gaccacgacc tcgctgggcc cggccaccat
     1501 gtcgatcccc acctcgccaa agacccggct cttggcgagg gtcacgtaga cgttgccggg
     1561 cccgacgatc ttgtcgaccc tgcggatcgt cggggtgccg cacgcgagcg cggccaccgc
     1621 ctgggcgccg ccgacgcggt agccctcggt gacgccggcg atccgcgcgg cggcgagcac
     1681 cgtcgggttc acgcggccgt cggatgccgg cggcgtgacg agcacgatct cggggacgcc
     1741 ggcgacgcga gcgggcacgg ccgtcatgag gacggacgat ggatacgcgg cgcggccgcc
     1801 gggcacgtag acgcccacgc gctcgagagg gcgcacctcc tggcccagca cggagccgtg
     1861 ctcgtcggtg attcgccacg acttcggcag cgcggcggcg tggtagcgct cgatgcgctc
     1921 ggcggcgtag gcgagggcgg cgcgggtcgc gggcgcgagc gcccgctcgg cggcctcgaa
     1981 ctccgcgggg gcgatgcgca ggtcggcggc cgaccgagcc ttgaagccat cgtacttctc
     2041 cgtcgtgaag agcagcgcat cgtcgccgcg ctcccggact gcgcggagaa tcgtctcgac
     2101 ggtgcggtgc tgctcgggcg tcgccccgcc cggcgtccgc tccagggcgg ccaccaccgc
     2161 gtcgagaccc agcgtcttca gatcgaggac acggatcacc ggctcacccc cgccggccgc
     2221 gcccgcatct cttcgatcag ctccgtcacg gcgcgatact cggtcttcat cgaggcgcgg
     2281 ttgacgatga ggcgcgccgt ggagcgggcg atctcggcga cctcgacgag gccgttcact
     2341 ctcagcgtct cgccggactg cacgagatcc acgatgcgct cggccaggcc caccagcggt
     2401 gccagctcga tcgagccgtc caggcgcacg atctccacct gcaccccgcg ttcggagaag
     2461 taacgctcgg ccatcctggg atatttcgtg gccacccgaa cccatgacca cttcgacggg
     2521 tcgtcgcgct cccacagctc gcggggctcg gcgacgacca gccggcagaa cccgaatcca
     2581 aggtcgagcg gctcgtacac gtccggttcc tgctcgagca ggatgtcctt gccgacgatg
     2641 cccaggtcgg cggcgccgta tgtgacgtag gccggcacgt cggccggctt gaggaagagc
     2701 agccgcagct cgcggacggg gtcgacgtgg atgagcttgc gcgacgcagc gtcgacgccg
     2761 tccacgccga gcgcgcgcag gaggccgagc gcaccctcga ggagacggcc cttgggcaag
     2821 gccagcgtga gccgctcgtt catcctcgcc gctcgatgca ggcgccgagg gccgcgagct
     2881 tgccttccat gcgctcgtag ccgcggtcga ggtggtacac gcgcgcgatc tccgttcgcc
     2941 cgcgggcggc gagtccggcg aggaccagcg cggcggaggc ccgcaggtcg gacgccatca
     3001 ccggtgcccc ctggtaggca ggcactccgc ggatgatcgc cgtggagcct tcggtctcga
     3061 tcgaggcgcc catgcgattc agctcggccg cgtgcatgaa gcggttctcg aagatcgtct
     3121 cggtcacccg cgactggccg ccggcgatcc cgagcagcgt catgagctgc gcctgcatgt
     3181 ccgtcgggaa cccggggaac gggctcgtgg tcacgtcgga gggccgggcc cgctccgggc
     3241 cccgcgcgcg cacacggtcg gccccgaccg tcagctcgac tccgcactcc tcgagcttgg
     3301 ccagcaccgc cgtgaggtgc tcggacacga gacccgtcac ggtcacgtcg ccgcgcgtga
     3361 tggcgccggc cacgatcagc gtgccggcct cgatccggtc ggggacgatc gtgtgggtcg
     3421 cgccgccgag gtcggacacg ccctcgatct cgatgcgcga ggtgccagcg ccgtggatgc
     3481 gggcgcccat cgcggtgagc agccgggcga ggtcaacgac ctccggctcg cacgccgcgt
     3541 tctcgatgac cgtcgcgccc tgggccagcg cggccgccat caggaggttc tccgtcccgg
     3601 tcacggtgac caggtcggtc gcgatgcgcg cggccttgag gcggctcgcg cgcgccacga
     3661 cgtagccgct ctcgatggcg atctcggcgc cgagcttcgc caggcccttg aggtgctggt
     3721 cgatcgggcg gaccccgatg gtgcaaccgc ccggcagcgc cacccgcgcc gacccgtggc
     3781 gggcgaccag cgggcccagc accagcaccg acgcgcgcat ggtcgagacc agcgcgtagg
     3841 gcgcaacgtc gctcgagacc cgccccacgc gcagggtcgc gcgctggccg tcgcgctcga
     3901 ggcgcgcgcc cagcgtctcg agcagccgcc ccatggtctt gacgtccacg aggtcgggca
     3961 cgttggtcag cgtgaccggc tcggaggtca ggagcgccgc ggccatggcc gggagcgcgg
     4021 cattcttcgc ggcgctcacc gccacggttc cgcggagcgg gacgccaccc tcgatcaaga
     4081 gccgagcggg catacgttgg agggggcctc gacggccctc tccggggccg gcccccccag
     4141 gagaggcggc ggcggcttcc ccgggcgtgc gttcacgccg gtcacgaagc gctgcgcgcc
     4201 ggtgaggtcc aggtgcagcg tcacatcgac gaagcctgcc tcacgcatga gcgcggcgac
     4261 gtcgggcgcc tgatcgccgc ccgcggtctc gagcgccagg gcgccgccgg gcgcgagccg
     4321 ggccggggcc tccgagacga gacgccggat caggtgcatg ccgtcggcgc cgccgtcgag
     4381 cgccagccgc ggatcgtgct tcgcgacctc gggcgcgagc ccggccagca tcgcgctcgg
     4441 caagtagggc gggttggaca cgatgagatc gctgcgcgtc gggccgagcc ccgcgaagag
     4501 atccccgacc acgacgaaga cgcggtcggc gacgccgacc gccctgacgt tccgacgcgc
     4561 aagtcggctt gcggcctccg acaggtccac cgcgacgaca cgcacgtcgc gtcgcgccgc
     4621 cgcgagggcg cacgcgatgc cgccggcgcc cgtcccgacg tcgatgacgc ggcgagcaat
     4681 ggcgcccgcc ggcgggagca atgcgagggc ccgctcgacc agggtctcgg tctccggacg
     4741 cggaaccagc acgtccggtg tgacggcgag ccgcagcccg cagaagtcct cccagccgag
     4801 gatgtgctgg agcggctcgc gccgcgcccg ccgccccacg gccgcctcga agcggccggc
     4861 gaccgccggc gtcaggccgg cggcgagacg agcgccgagc gcggcacggc ccacgccgag
     4921 gatgccggcc agcagccact cggcgtccac gcgcggcgtg tcgatgccgg cgccggccag
     4981 tcgcgcggtc gcagccgcga gcgcggcacg catcgagggc acggcgaccg ccgggctcac
     5041 tgcgccacct tctcgagccg ctcggcctgc tcggcggcgg cgagggcgtc gatcagctcg
     5101 tcgaggtcgc cctcgagcac ggccggcagc cggtgtagcg tgagcccgat gcgatggtcg
     5161 gtcacgcgcg tctggggaaa attgtaggtg cgaatccgct cgcttcgctc gccggtgccg
     5221 acctggctcc tccggtcggc ggcgatcgcc gcctgctgct cctcctgcgc gcgctcgagc
     5281 aggcgcgcct tgagtacgcg catggccttt gcgcggttct tgatctgcga ccgctcgtcc
     5341 tggcaggtca cgacgaggcc ggtcggcagg tgcgtgatcc gcaccgccga gtccgtcgtg
     5401 ttgacgcctt ggccgccggg acccgacgag cggtagacgt ccacgcgcag gtccctctcg
     5461 tcgaccttca cctcgacgtc ctcggcctcg ggcaggacgg cgaccgtcac cgtggaggtg
     5521 tggatgcgcc cggcagactc cgtcaccggc acacgctgca cgcggtgcac accgcgctcg
     5581 aacttcagcc ggctccaggc gccccggccc tggatgaaca ggatgacgtc tttcaggccg
     5641 ccgacgccgg tggggctcgc gtccatcacc tccaccttcc agcgctggcg ctccgcgtac
     5701 ttcgtgtaca tccgggccag gtcggccgcg aagagcgccg cctcgtcgcc gccggcgccg
     5761 ccgcggatct cgacgaacac gttcttgtcg tcgttgggat cgcgcggcac cagcaggcgc
     5821 ttgagcgcgg tctcgagcgc ggcctggcgg gcggtcagct cgtcgctctc cgcctgggcc
     5881 agctccagca gctcgcggtc gccgccctcc tcgaggatgt gacgggcctc gcccaggcgc
     5941 ttgagcactt cccggtactc gcggaagcgc tcgacgatct cctgggtctc ggcgtgctcc
     6001 ttgcgaaggc gggcgaactc ggcctggttc ccgtagatct tggggtcggc cagcgcccgg
     6061 gccagctctt ccgagcgctc ctcgatctgc ctcagcttgt caaacagcat cgtggatcac
     6121 ccggcctgcc cgcgagccgg ccggcggctc agctcggggt ggtcgtctgc cgcttgtact
     6181 tgcgctggaa ccgctcgacc cgccccgccg tgtccatgag cttctgcttg ccggtgtaga
     6241 acgggtggca cttcgagcag acctccaccc ggatctcggg cctcgtcgag cgggtcttga
     6301 tgatttcccc gcaggcgcag ctgatcgtgg tgtcgacgta ctccggatga ataccagcct
     6361 tcacggccta ccccctttgg agcctcagca taccaccccc gtcggaatct gccaaaacgg
     6421 cgggctgtta gatcgcgccg cccgtccagg gcgggcgacc gtcacccttc cggctccagg
     6481 tcgagcgtct cgcgcacctt gcgcatgaac gcgtcgagcg tgaatggctt ggggagcagg
     6541 ggggtgccgg gcgcgagccc gcctcggtgg acgatctcgt cctcggcgta gccggacatg
     6601 tagagcacgc gcatctccgg acgctcggcc tggaggcatt cggccagcgc gcgcccgccc
     6661 atccggggca tgacgacgtc ggtgagcagc agatggatcg gcccgtcgtg ccgccggacc
     6721 aggtcgagcg cttcggcagg gccggacgcg gcgagcgtct cgtagccgta cgacgtgaga
     6781 acctggtgcg cgagctcgcg caccagatcc tcgtcctcca ccagcaggac cgttcgcggg
     6841 gctggcggtg acgctgtcgc ctcgtcgtcc ggcccgcccg gctcaccggc gtcgtccacc
     6901 cgctggagat agatccgaaa cgtcgcgccc acgtccggcg cgctttccac gccgatggag
     6961 cccccgtgct gcttgacgat gccgtacacg gtcgcgaggc cgagcccggt tcccctgccg
     7021 acttccttgg tggtgaagaa cggttcgaag atgtgcgcct ggacctccgg gctcatgccc
     7081 gagcccgtgt cgctcacctg gagcagcacg tgcgccccgc gggacgagtc gggatgggcg
     7141 gcgacgaagt cgtcgtcgag gtggacgttc gccgtctcga tcctgagccg tccgccccgg
     7201 ggcatcgcgt cgcgcgcgtt gacggcgagg ttgaggatga tctgctcgat ctgggtgggg
     7261 tccgcgtgca cggctccgag gtcgggagcg aggcgcgtga ccaggtgaac gtcctcgccg
     7321 atgagccgct ggagcatctg ggccacaccg gcgacgacgc gattcaggtc gagcacccgc
     7381 ggccggagca cctgccggcg gctgaacgcg agcagctgtt tcgtgagggt accggcccgc
     7441 tcggcggtcg tcttgatcag ctcgaggtcg cggcacgccg ccgcatcgaa cgccagccgc
     7501 tgcagcagga actcgctcct cccggtgatg atggtcacca ggttgttgaa gtcgtgggcg
     7561 atcccgcccg cgagccggcc gaccgcctcc atcttctgcg cctggcgcag ctcggcttcg
     7621 ctcgcccgga gggcggcttc cgcccgggcg gcctcgtcgt gcaggagggc ccggtcgagc
     7681 gcgctcgccg ccaggtcggc aaacgcctgg aggcgggcgg tatcgcgctc ggagacggag
     7741 cggcccgcct cgaagcccag cgacagtgcg ccgatggtgt ggcctccggc gacgagcggc
     7801 accgcgagca ccgagcgcag ttgggtcccc gtgatgaacc ggcggcgctc ctcgtcgatg
     7861 acgatgcggg aatcgtcgag gatgtccggc gaccggcagg ggcgcccctc gcgcatggcc
     7921 gtctccagca gctcgcggcc cgggcggacc cgctcgtccg cagaagccag agaccccacg
     7981 gcgtcgtgcg ccgccagcat gaccagcgcg ccgtcgctgt cgagccgcgc gacgcacgtt
     8041 gccagagcat cgaacatcgc caccaggctc tcggcgacgt gcgtggccac ggccgagacg
     8101 ccgagggccc cggccatcgt ccgcgccacc tgcgccagct gctcggcctc ggaccgacgc
     8161 cgctcggcgt cttcgtgaag ctgcgcgttg tcgagcgcga tcgccgcctg atcggcgaag
     8221 gcctgcacca gcctgacctc ggcgtccgtg aacgcccggg ggcgccgatc ggcaaccgtg
     8281 agggcgccga tcgtccggcc ccgtggccgc agcggcaccg ccagcagcgc ccgctggccg
     8341 ccggcgacga ggcgatcccg gatgcccccg ggccagacga atcgcgtatc gcccagcaca
     8401 tcgccggacc acagcggccg gctctcctgt accgcccgtc ccgtcacccc gacaccccga
     8461 ggcaggacga gaccctcgct cgccgtcagg gggtcgccgt cgcgcgccag gacgtgcagg
     8521 ttgccgtccg gatcgagcag ggtgatggcc gaggacctga tcggcagcag ggtcaggacg
     8581 tgctcgacga tccactggca aacccccacg acctcaagcc gctcgttgag caggcgcgcg
     8641 acccgcatca acgcttcggc ctctcgagcc ccacgctgag atgcgacggg ccggcggccc
     8701 gtccccgccg gcgaccggcg cggccggcgc ttcggtacgt cagcgtcggt catcttcagc
     8761 cgcactccga cgtccgtggc gaggccccgg caacctggcg aacacccgag gcaggtcgtc
     8821 gaggcaatga gcgtggtgag agtcggcagg ccggctcgag cgaggaactc gcgcgcgcac
     8881 gtttcgcacg gtttcagctt ggcgcaggac cggccggagc gacatcgggg ccgacacgag
     8941 atccgtcggg acgacccctg agctccgacg ggggaaagac ctgagtcctc gcgccgcgtc
     9001 atcgagacgc gcacggctgt gccggggagc cgagcgccag gggcttgggg gccatgtcgg
     9061 gccccagatg tctatcgtcc cggctcagga ctccggggtg atgagcccaa ccctgatggc
     9121 gaagcgcacc aggcccgcca cgtcgcggat gtcgagccgg tccatgagct gggcgcggtg
     9181 tgtctcgatc gtcttgacgc tgagcttcag cttggcggcg atgtccttcg tcgtcaggcc
     9241 ctcggcgacg agctggagca cctcgcgctg gcgggacgtc agccgctcga ggggatcggg
     9301 ctgatcggcg acgcgccgcc ggtagtcggc gatcaggtgc ttggacatgg cggggctcag
     9361 ataggtctcg ccgcgcgcga ccgcggagat ggccagctcg agctccgcga ggtcggcgtc
     9421 cttgaggaga tacccggcgg cgcccgcccg gagcgcctgg agcgcgtact cctcggcggc
     9481 gtgcatggac aggatgatga ccgcggtgcg cgggtgggcc ttgacgatgc gggcggtggc
     9541 ctcgagcccg ttgaggccgg gcatggcgat gtccatgagg acgaggcgcg gatgcagcgt
     9601 ctcgaccagc cggacgacct cgtggccgtc ccgggcctcg gccacgacct cgacgcctcc
     9661 gatcgcggac agcagcgatc ggatgcccgc gcggaccagc gcgtggtcgt cggcgagcac
     9721 gacgcggatc g
//
LOCUS       DATIAZ010000329         3313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1675908, whole genome shotgun sequence.
ACCESSION   DATIAZ010000329 DATIAZ010000000
VERSION     DATIAZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675908"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VLF19_11385"
     CDS             <1..422
                     /locus_tag="VLF19_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93899.1"
                     /translation="RALLEVVRSASEQAPRHPGPPVTSLSIDRLRLPSDSHEVLLARI
                     DATLIFAQPRGPRTEELAARTVQLCRLAAAMLTRGNLGPMCHAVIEGAAETTHLRADS
                     QVVLLMAFGRDVEVAAGLAHLERVWSNCRQELASVLT"
     gene            422..832
                     /locus_tag="VLF19_11390"
     CDS             422..832
                     /locus_tag="VLF19_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HSE93900.1"
                     /translation="MAVSAHGHPEPAAETPARGRAFRQLLRDLVKLEHVRGGLIVAPD
                     GLVIASDLPREVPVEPLSALGAALGRELELRGPRLRGGTFLMAHFAAGDGTVFLGGTP
                     IGFIVLLGGAEVSRDAVRHALRTALDSVREAWGR"
     gene            complement(902..2389)
                     /gene="tcuA"
                     /locus_tag="VLF19_11395"
     CDS             complement(902..2389)
                     /gene="tcuA"
                     /locus_tag="VLF19_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HSE93901.1"
                     /translation="MTATVRDVVVVGGGNAAMCAALSAREQGARVLVLEKAPEAWRGG
                     NGFFTAGGFRFAFKSFDELREIVRDMSDEEAASMEVDPYTEDEFYDDLMRVTEDCADP
                     DLALTLVRESQPTVRWMRDRGIRWIPMFGRQAFKIGSRFRFWGGLVLEAVGGGPGLID
                     MAYASAEKAGIDVRFEAKATRLVVDDRGAVTAVVVRTPAGTETIVARAVVLAAGGFEA
                     NPEMRTRYLGPNWELARVRGTPYNTGDGIRMALDIGALPWGHWSGCHSVQWDLNAPWH
                     GDRKVGDNFQKHSYPVGIIVNLRGERFVDEGADFRNYTYVKYGRAVIAQPRRAAFQIF
                     DQKVLHLLREEYRIREVTKAEDMTLEGLAHKLEIDVAGFVETVTRYNAAVQPGAFNPA
                     VKDGKHTRGITPPKSNWAQPLDTPPYVGFAVTTGITFTFGGLRITGDAQVIDCEQRPI
                     PGLYAAGELVGGLFYHNYPGGAGLMAGAVFGRIAGRAAAKAALGA"
     gene            complement(2391..2705)
                     /locus_tag="VLF19_11400"
     CDS             complement(2391..2705)
                     /locus_tag="VLF19_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HSE93902.1"
                     /translation="MIRMSVLYPGGAGKKFAIDYYATKHMALVRSRWSGMGLVKVEVD
                     KGLAGGAPGSPAPFVAAGHVYFSSLDDLQKATTAHGKELFADVPNFTDITPQVQISEI
                     VG"
     gene            complement(2783..>3313)
                     /locus_tag="VLF19_11405"
     CDS             complement(2783..>3313)
                     /locus_tag="VLF19_11405"
                     /inference="COORDINATES: protein motif:HMM:NF022083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HSE93903.1"
                     /translation="LLVTQPDMSGEPLAGGRVTLMGEGRRLPEGEVGVARETYLARQA
                     NAAYWADFEDFAFFRLEVTDVYFVGGFAAMGWVGAGEYRAARPDPLADAASSLLEHMN
                     RDHADALIEYARFFAGVEADEATMAAVDRLGFKLRLRQGHRRWSVRIGFPRGVTSAGE
                     TRTTLLEMLRQARAGR"
BASE COUNT          451 a         1188 c         1184 g          490 t
ORIGIN      
        1 cccgcgcgct gctggaggtg gtccgctcgg cgagcgagca ggcgccgcgg catccgggcc
       61 cgccggtgac gtcgctcagc atcgaccgct tgcgcctgcc cagcgacagc cacgaggtgc
      121 tgctggcgcg aatcgacgcg acgctgatct ttgcccagcc gcgcggcccg cggacggagg
      181 agctggccgc acgcaccgtg cagctgtgcc ggctcgcggc ggcgatgctg acccgcggca
      241 atctgggacc gatgtgccac gccgtcatcg aaggcgccgc ggagacgacg catctgcgag
      301 ccgacagcca ggtggtcttg ttgatggcgt tcgggcgcga tgtcgaggtc gccgcgggcc
      361 tcgcgcatct cgagcgcgtg tggtcgaact gccgccagga gctggcgagc gtgctgacgt
      421 gatggcggtc tcggcgcacg gtcacccgga gcctgcggcc gagacgccgg cgcgcgggcg
      481 tgccttccgt cagctgctgc gcgatctggt gaagctggag cacgtgcgcg gcgggctgat
      541 cgtcgccccc gacgggctcg tcatcgcctc cgacctcccg cgcgaggtgc ccgtcgagcc
      601 gctgtcggcg ctcggggccg ccctgggccg ggagctcgag ctgcgcggac cgcgcctgcg
      661 cggcgggacg tttctcatgg cccatttcgc cgcgggcgac ggcacggtgt tcctcggcgg
      721 aacgccgatc ggcttcatcg tgctgttggg cggggccgag gtgagtcgcg acgccgtccg
      781 ccacgcgctg cgcaccgcgc tggacagcgt gcgcgaagcc tggggccgct gatcaggcgc
      841 ggagcgcggt gcgcgctccg cgagcacctc gtcggtgagc ccggtgcccg ggcgggccaa
      901 ctcaggcgcc gagggccgcc ttcgccgccg cgcggcccgc gatccggccg aagacggcgc
      961 ccgccatgag cccggcgccc ccgggatagt tgtggtagaa gagcccgccg acgagctcgc
     1021 cggccgcgta gagccccggg atcggccgct gctcgcaatc gatcacctgg gcgtcgccgg
     1081 tgatccggag cccgccgaac gtgaaggtga tacccgtggt caccgcgaag ccgacgtacg
     1141 gcggcgtgtc gagcggctgg gcccagttcg acttgggcgg cgtgatcccc cgcgtgtgct
     1201 tgccgtcctt gacggcgggg ttgaacgcgc ctggctgcac ggcggcgttg taacgggtga
     1261 cggtctcgac gaagccggcg acgtcgatct cgagcttgtg ggcgagcccc tcgagcgtca
     1321 tgtcctcggc cttcgtgacc tcgcggatgc gatactcctc gcgcagcagg tgcagcacct
     1381 tctggtcgaa gatctggaag gccgcccgtc ggggctgcgc gatgacggcg cggccgtact
     1441 ttacgtaggt gtagttccgg aagtccgccc cttcgtccac gaagcgctcg ccgcggaggt
     1501 tgacgatgat gccgaccggg taggagtgct tctggaagtt gtcaccgacc ttccggtccc
     1561 cgtgccacgg cgcgttgagg tcccactgga cggagtgaca gccgctccag tgcccccacg
     1621 gtagcgcgcc gatgtccagc gccatgcgga tgccgtcgcc cgtgttgtac ggcgtgccgc
     1681 gcacgcgggc cagctcccag ttcgggccta gatagcgcgt gcgcatctcc ggattggcct
     1741 cgaagccgcc ggcggcgagg acgacggccc gcgcgacgat cgtctcggtg cctgccggcg
     1801 tgcgcacgac gacggcggtg acggcgccgc gatcgtccac gacgaggcgc gtcgccttgg
     1861 cctcgaagcg cacgtcgatc cccgccttct ccgccgacgc gtaggccatg tcgatcagcc
     1921 cggggccgcc cccgacggcc tccagcacga ggccgcccca gaaccggaag cggctgccga
     1981 tcttgaacgc ctggcggccg aacatcggga tccagcggat gccgcggtcc cgcatccagc
     2041 ggaccgtcgg ctgggactcc cgcacgagcg ttagcgccag gtcggggtcg gcgcagtcct
     2101 cggtcacgcg catgaggtcg tcgtagaact cgtcctcggt gtacggatcg acctccatcg
     2161 acgcggcttc ctcgtcgctc atgtcgcgga cgatctcgcg cagctcatcg aagctcttga
     2221 acgcgaagcg gaagccgccg gccgtgaaga agccgttgcc gccgcgccag gcctcgggcg
     2281 ccttctcgag cacgagcacg cgcgccccct gctcgcgcgc ggagagcgcc gcgcacatcg
     2341 cggcgttgcc gccgcccacc accacgacgt cgcgcacggt cgccgtcatg tcagcccacg
     2401 atctcgctga tctgcacctg cggcgtgatg tccgtgaagt tcggcacgtc cgcgaacagc
     2461 tccttgccgt gcgccgtggt ggccttctgg aggtcgtcca acgagctgaa gtacacgtgt
     2521 ccggcggcca cgaacggcgc gggcgacccc ggcgcgccgc cggccagccc cttgtccacc
     2581 tcgaccttca ccagccccat gccgctccag cggctcctga ccagagccat gtgcttggtc
     2641 gcgtagtagt cgatggcgaa cttttttccg gcgccgcccg ggtacaggac cgacatgcgg
     2701 atcatgggca acctcctcga ggagcgatgg tgaggcgtac cctatgcgtg gccgttggag
     2761 gagtcaagtc agcgggccgg cgtcaccggc cggcgcgtgc ctgccggagc atctcgagca
     2821 gggtcgtccg ggtctcgccg gcgctcgtca ccccacgggg aaatccgatg cgcacgctcc
     2881 accgccggtg gccctgccgg agccggagct tgaagccgag gcgatcgacg gcggccatcg
     2941 tggcctcgtc ggcctcgacg ccggcgaaga agcgcgcgta ctcgatgagc gcgtcggcat
     3001 ggtcccgatt catgtgctcg aggagggagc tcgcggcgtc cgcgagcgga tcgggccggg
     3061 ccgcgcggta ttcaccggca cccacccagc ccatcgccgc gaagccgccg acgaagtaga
     3121 cgtcggtgac ctccaggcgg aagaaggcga agtcctcgaa gtccgcccag taggcggcgt
     3181 tcgcctgccg cgccagatag gtctcgcgcg ccaccccgac ctcgccctca ggaagccggc
     3241 ggccctcacc catcagcgtg acgcggccgc cggccagcgg ctcaccggac atgtcgggct
     3301 gcgtgacgag cag
//
LOCUS       DATIAZ010000330         9066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1542723, whole genome shotgun sequence.
ACCESSION   DATIAZ010000330 DATIAZ010000000
VERSION     DATIAZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542723"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VLF19_11410"
     CDS             <1..925
                     /locus_tag="VLF19_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSE93904.1"
                     /translation="HATGTRDMEEMGGLIKRMPWTAACFLVGSAAIAALPPLNGFVSE
                     WLTFQALLQNLKIPRPELNLVFALAIAGLALTGGLAMACFVKAFGITFLALPRAEAAA
                     RAHEASPAMRAGMVILAAACVALGLAPTLMVPALVAIAGSVMDTPASPRVADWLTLTV
                     SGEFASLSTLTVAAALAGGALLPLAVLALVGAGRRRPYETWGCGRLVQTPRMEYTATG
                     FAYPFKRVFDFFYRPVKQLDIEVHPESRFFVRKLEYENPTRSIFEDWLYRPVLDALHH
                     GARLARALQSGSANLYLMYILAALLAMLVLA"
     gene            922..1866
                     /locus_tag="VLF19_11415"
     CDS             922..1866
                     /locus_tag="VLF19_11415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HSE93905.1"
                     /translation="MIADLVGAVVQVAVVGGGAPLLVGLLRTLKARLVGRRGPRPWQP
                     YADLAKLLRKEAVVSTTTSWIFRVTPYVLTATMLTAALVVPVVVSRPALAFAGNVILL
                     MYLFMLGTFFLALAGLDAGSAFGGMGSSREAAVAALAEPTVIVAVFALALRADTINLG
                     GVVERFAREPQLVANPGHLLAFAAFFIVMIAETGRLPVDNPATHLELTMIHEAMVLEY
                     SGHYLALVEWAGAMKLLLFLTLLANLFFPWGVPAEVGVVPLVLGALALAGKLVLLTAL
                     LAAIETSVAKLRLFRVPELLAGSFALALLSVTSVFLLR"
     gene            1877..2518
                     /locus_tag="VLF19_11420"
     CDS             1877..2518
                     /locus_tag="VLF19_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93906.1"
                     /translation="MTAATNLLAFLGLVVTLLIVWRQSRPGRLRLFVVQSLLLAALAV
                     AIGLFAGKPGLLVVAGVVVALKAWLIPRALARIGAGTPVRPVAPGRTTALPLLVAGAL
                     VVVAYVVMLPVTGATELPTEGAIPLAFAVALIGLLVTVTGRDAFGHTLGFLMFETGIF
                     GLALLVTYGLPGLVEAGVFLDVLAIVLLMEGVVLQLRREDEAADANRPEEVSA"
     gene            2515..3945
                     /locus_tag="VLF19_11425"
     CDS             2515..3945
                     /locus_tag="VLF19_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSE93907.1"
                     /translation="MMLLVLLAVPLAAAAALARVRGAAAGAVHTGGALLTLASAGALG
                     VTVVREGSVTALDGLLRADALSAWMAALVALVAALAAAEAPRHVPLAAGGRFYPLLHL
                     FTFTMLLAVTTDDVGLLWVAVEGTTLASVFLVNFERSRASLEASYKYLLICSVGVAIA
                     FVGTVLVYFTDVQQFGHEPHALRWTTLVRLAPGLPPRVVELAFVFLVIGYGTKAGLAP
                     MHTWLPDAHSEAPAPVSALMSGVLLGVGLYAVLRFKTVADLAAGPAFAQRLLVGLGLV
                     SLAVAAAFLWAPRNYKRMLAYSSIEHLGIACLGVGFGGAWGLGGAVLHVANHALAKSV
                     LFLLSGRIRAAYGSAEIPAVSGLLGSMPFTGRAFFVAMLALMGLPPFGLFVSEVMIFG
                     AGFAQGWPVAATTGLVLLVVAFAGLLGAAHRMVYGATPARPPETGGWIGGIPLVAALA
                     LLVLTGVAWPPGLAGALERIAAVLAP"
     gene            3942..5519
                     /locus_tag="VLF19_11430"
     CDS             3942..5519
                     /locus_tag="VLF19_11430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSE93908.1"
                     /translation="MTLEPEVIRHALEAAGATEVTRGGDGTLAGRLAPGRVPGLADVL
                     RRRGLEVQLLAATDTRATAAGFTLTYVFAPPGGCPAATVLATLPSGATRFPSLATRSY
                     AASRFEREIHDLFGLVPTGHPDLRRLALHQFWPAGYHPLRRDVASRRDFADEGQPFPF
                     RRVEGPGLFEITVGPVHAGIIEPGHFRFTVEGETIVNLETRLGFVHKGIEKLFETLPL
                     ARAHELAERVSGDESVAHAAAFCQALESLAGGVVPARGAWLRVVLLELERLYNHVGDV
                     GMIVNDTGFAFGHAHCFRLREALLRLNERMTGHRLLRGAVVPGGVTGPVERADLGDLA
                     SEVGRLVEEFGGIAGLCLDNTMVLERLQGTGRLTERTAREMQVVGLAGRASGIDADAR
                     RDAPSAAYGHLDVRVAVHDAGDVWARTMVRLHEARESARLLALAAERAPAGPARASLP
                     PLPAGGHATGVVEAWRGPVWYWVQAEGPDRLARVKAADPSFRNWPALEYAVLKNIVPD
                     FPLCNKSFNLSYSGSDL"
     gene            5587..7317
                     /locus_tag="VLF19_11435"
     CDS             5587..7317
                     /locus_tag="VLF19_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HSE93909.1"
                     /translation="MIVHVLAVDYDGTIAERGSVSPTTAASLARVRASGRKCVLVTGR
                     MLPDLRRVCPEVDAMFDAVVAENGALLYLPARREVRALGNAPEPTLVDALSRRGVRHD
                     LGTSIVASDDEYAEAALAAIRETGVERSLVFNKGSLMLLPGGVTKGTGLTAALETMEL
                     SPHNLVGIGDAENDHAFLALCECAVAVADAVPALRERADHVTSGGAGAGVAEFVEGHL
                     LADAADVVPRLARHQIPLGQTPGGEAVTVPAHATGLLIVGPSATGKSTLTGVLVERLI
                     EAGRAFCLLDPEGDHDPLVELPGVVALGGTGRQTLPGAEELRQLLRQPACRLVLNLSG
                     LSMSDKVSYATHALGAVAAVRSASGLPHWLIVDEAHHIAPADGSSAVELLPPAGESLA
                     LITLSADRLARAARRAIGAVASTDVEAFREALATLRADRMTGARLPDVPGGVLERGEA
                     VLASLTAGAARVARFEVARRQVEHRRHVRKYAEGELPPDRSFYFRGPRDALNLRAVNL
                     KRFCELAEGVDEATWTHHLGRREYSAWMRAMIKDRELAEEVAAVEASPGEPAESRRRV
                     LEAVRRRYSV"
     gene            complement(7410..7994)
                     /locus_tag="VLF19_11440"
     CDS             complement(7410..7994)
                     /locus_tag="VLF19_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE93910.1"
                     /translation="MRGVEQVPWIYDAMCAVLERTGLGRWRRWLADGARGRTLDLGCG
                     TGRTLPLFPASVRVVGTDPVRESLRRARRRAPDVPLVQASAEALPFGDGVFDTVVSSL
                     VFCSVPDPARGLSEVRRVLAPGGTLRMIEHVRSTRRWKARFQDAVQPLWTRIAGGCRP
                     NRETERTVEANGFVIEAESRRARADMRRFVARAR"
     gene            complement(8091..8693)
                     /gene="pncA"
                     /locus_tag="VLF19_11445"
     CDS             complement(8091..8693)
                     /gene="pncA"
                     /locus_tag="VLF19_11445"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HSE93911.1"
                     /translation="MTLVIDPSRDALVVVDVQNDFCPGGSLAVPEGDRVVPVLNRYAA
                     RFAASGAAVFASRDWHPERTRHFAAQGGAWPPHCVQGTPGAEFHPALSLPAGAEVVSK
                     GMDPGDDAYSCFQAEDAQGMPFAAALGERGVGRLFVGGLATDYCVKATVLDAMKEGFE
                     VVVLEDAVRAVDVSPGDGARALEEMRRAGAALARFDDVGG"
     gene            8808..>9066
                     /locus_tag="VLF19_11450"
     CDS             8808..>9066
                     /locus_tag="VLF19_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93912.1"
                     /translation="MKRLLTLVAVVALVLTAGFYAIAQQPYQLTPMDEAKRDRAVSVG
                     GGKYHVLPSTLETTQWGWLDPNEKPKLVISSGDTVAIETMMH"
BASE COUNT         1028 a         3454 c         3244 g         1340 t
ORIGIN      
        1 ccacgcgacg ggcacgcgag acatggagga gatgggcggg ctcatcaagc ggatgccatg
       61 gaccgccgcc tgcttcctcg tgggctcggc ggcgatcgcc gcgctgccgc cgctcaacgg
      121 cttcgtgagc gaatggctca ccttccaggc gctgctccag aacctgaaga tcccccgccc
      181 ggagctgaac ctggtcttcg ccctcgccat cgccggcctc gcgctcaccg gcggcctggc
      241 catggcctgt ttcgtgaagg cgttcggcat caccttcctc gcgttgccgc gcgccgaggc
      301 cgccgcgcgc gcccacgagg cctcaccggc gatgcgagcg ggcatggtga tcctggcggc
      361 ggcctgcgtc gccctcggtc tcgcgccgac cctgatggtc ccggcgctgg tcgccatcgc
      421 cggctccgtc atggacacac cggcgtcgcc cagggtcgcc gactggctca ccctcaccgt
      481 gtcaggggag ttcgccagcc tgtcgacgct caccgtcgcc gcggccctgg ccggcggcgc
      541 gttgctgcca ctcgccgtgc tggcgctcgt cggggcgggc cgtcggcgac cgtacgagac
      601 gtggggctgc gggcgcctcg tgcagacgcc gcggatggag tacacggcca ccgggttcgc
      661 gtaccccttc aagcgcgtct tcgatttctt ctaccggccg gtgaagcagc tcgacatcga
      721 ggtgcacccg gagtcgcgct tcttcgtgcg gaagctcgag tacgagaacc cgacgcggtc
      781 gatcttcgag gactggctct accgcccggt cctcgatgcg ctgcaccacg gcgcccggct
      841 cgctcgcgcg ctccagtcgg gcagcgccaa cctctacctc atgtacatcc tggccgcgct
      901 cctggccatg ctggtgctgg cgtgatcgcg gacctcgtcg gtgccgtcgt ccaggtcgcc
      961 gtggtcggtg gtggcgcgcc cctgctcgtc ggcctgctgc ggaccctcaa ggcgcgcctc
     1021 gtcggccggc gcgggccgcg accgtggcag ccctacgcgg acctcgcgaa gctcctgcgc
     1081 aaggaggccg tcgtgtcgac gacgacctcg tggatcttcc gcgtcacccc gtacgtgctc
     1141 accgccacca tgctgacggc cgccctcgtc gtccccgtcg tggtgagccg accggcgctc
     1201 gccttcgcgg ggaacgtcat cctcttgatg tacctcttca tgctcgggac cttcttcctc
     1261 gcgctcgcgg gcctcgacgc cggcagcgcc ttcggcggca tgggctcgag ccgcgaggcc
     1321 gcggtggccg cgctcgccga gcccacggtc atcgtggccg tgttcgccct ggcgctccgc
     1381 gccgacacca tcaacctcgg cggcgtcgtc gagcgcttcg cccgcgaacc gcagctcgtc
     1441 gccaacccgg gccacctgct cgccttcgcg gcgttcttca tcgtgatgat cgcggagacc
     1501 ggccggttgc cggtggacaa ccccgccacg cacctcgagc tgacgatgat ccacgaggcc
     1561 atggtgctgg agtactcggg ccactatctg gccctcgtgg agtgggcggg cgcgatgaag
     1621 ctcctcctct tcctcacgct cctggccaac ctgttcttcc cgtggggcgt gccggccgag
     1681 gtcggcgtgg tcccgctcgt gctgggtgcg ctcgcgctcg ccgggaagct cgtgctgctg
     1741 acggcgctgc tcgccgccat cgagacgtcc gtggcgaagc tgcggctctt ccgcgtcccc
     1801 gagctgctgg ccggctcctt cgcgctggcc ttgctgtcgg tcacctcggt cttcctgctg
     1861 cgatgagcgc ccgcccgtga ccgccgccac caacctgctc gcgtttctcg gtctcgtggt
     1921 gacgctcctg atcgtctggc gtcagagtcg gcccgggcgc ttgcgcctct tcgtcgtcca
     1981 gtccctgctc ctcgccgcgc tcgcggtggc catcgggctc ttcgcgggca agccgggatt
     2041 gctcgtggtg gccggcgtcg tggtggccct caaggcctgg ctgatcccgc gggccctggc
     2101 gcgcatcggc gccggcaccc ccgtgcggcc ggtcgcgccg ggccgcacga ccgcactgcc
     2161 cctgctcgtg gccggcgcgc tcgtcgtggt ggcctacgtg gtgatgctgc cggtgacggg
     2221 cgccaccgag ctgcccacgg agggcgccat ccctctggcc ttcgcggtgg cgctcatcgg
     2281 actcctggtg acggtgacgg ggcgcgacgc cttcggccac accctcgggt tcctcatgtt
     2341 cgagaccgga atcttcggtc tggccttgct ggtcacctac ggcctgcccg gcctcgtcga
     2401 ggccggcgtg ttcctcgacg tgctcgccat cgtgctcctg atggagggcg tggtgctcca
     2461 gctccgccgg gaggacgagg ccgccgacgc gaaccgtccc gaggaggtct ccgcatgatg
     2521 ctgctggtcc tgctggcggt cccgctcgcc gccgctgccg cgctcgcccg ggtgcggggc
     2581 gccgcggccg gcgccgtcca caccggcggc gccctcctca cgctggcctc cgccggtgcg
     2641 ctgggcgtca ccgtcgtccg cgagggcagt gtgaccgcgc tcgacggact cctgcgggcc
     2701 gacgcgctgt cggcgtggat ggcggcgctc gtcgccctcg tggcggcgct cgccgccgcc
     2761 gaggcaccgc gccacgtacc gctcgccgcc ggcgggcgct tctacccgct gctgcatctc
     2821 ttcacgttca cgatgctgct cgccgtcacc acggacgacg tcggcctgct gtgggtcgcc
     2881 gtcgagggga cgacgctggc gtcggtcttc ctggtgaact tcgagcgctc gcgcgcctcg
     2941 ctcgaggcgt cctacaagta ccttctgatc tgctcggtcg gcgtcgccat cgccttcgtc
     3001 gggacggtgc tcgtgtactt caccgacgtc cagcagttcg gtcacgagcc ccacgcgctc
     3061 cgctggacca cgctcgtccg cctcgccccc ggcctgccgc cgcgcgtggt ggagctggcg
     3121 ttcgtcttcc tggtcatcgg ctacggcacc aaggccgggc ttgcgccgat gcacacctgg
     3181 ctgcccgacg cgcacagcga ggcgccggcg cccgtcagcg cgctgatgtc gggcgtgctg
     3241 ctcggcgtcg gcctctacgc cgtcctccgg ttcaagacgg tggccgacct cgcggccggc
     3301 ccggccttcg cccagcgctt gctcgtcggg ctcgggctcg tctcgctcgc cgtggccgcc
     3361 gcgttcctct gggcgccccg gaactacaag cgcatgctcg cgtactccag catcgagcac
     3421 ctcggcatcg cgtgtctcgg ggtgggcttc ggcggcgcct ggggcctcgg cggcgcggtc
     3481 ctgcacgtcg ccaaccacgc gctggccaag tcggtcctgt tcctgctctc cgggcggatc
     3541 cgggcggcgt acggctccgc ggagatcccc gcggtgagcg gcctgctcgg gtccatgccg
     3601 ttcaccggcc gcgccttctt cgtcgccatg ctcgcgttga tgggcctgcc gcccttcggt
     3661 ctgttcgtga gcgaggtgat gatcttcggc gccggattcg cgcagggctg gccggtggcg
     3721 gccacgacgg ggctggtcct cctggtcgtg gcgttcgccg gcctcctggg cgcggcccac
     3781 cgcatggtct acggcgcgac gccggcgcga ccgccggaga ccggaggctg gatcggcggg
     3841 atacctctcg tggccgcgct ggcgctgctc gtcctcaccg gtgtggcgtg gccgcccggc
     3901 ctcgcgggtg ccctggagcg catcgcggcc gtgctcgcgc catgacgctg gagccggagg
     3961 tgatccgcca cgcgctggag gcggcgggcg cgaccgaggt cacgcgcggc ggggacggca
     4021 cgctcgcggg ccgcctcgcc cccgggcgcg tgccggggct cgccgacgtc ctgcggcgac
     4081 gcgggctgga ggtccagctc ctggcggcga ccgacacgcg agcgacggcg gcgggcttca
     4141 cgctgacgta cgtgttcgca ccgcccggcg gctgccccgc ggcgacggtg ctcgcgacgc
     4201 tcccgtccgg ggccacccgg ttcccctcgc tggccacgcg ctcgtatgcg gccagccgct
     4261 tcgagcgcga gatccacgac ctcttcgggc tcgtgcccac cggccatccc gacctccgcc
     4321 gcctggcgct ccaccagttc tggcccgcgg gttatcaccc gctgcgacgc gacgtcgcct
     4381 cgcgccggga cttcgccgac gagggccagc ccttcccctt ccgccgcgtc gagggccccg
     4441 ggctcttcga gatcaccgtg ggcccggtcc acgccggcat catcgagccg gggcacttcc
     4501 gattcaccgt cgagggcgag acgatcgtca acctcgagac gcggctcggc ttcgtccaca
     4561 aggggatcga gaagctcttc gagacgctcc cgctggcccg cgcccacgag ctggccgagc
     4621 gcgtgtcggg cgacgagagc gtggcgcacg cggcggcgtt ctgccaggcg ctggagtcgc
     4681 tggccggcgg cgtggtgccg gcgcgggggg cgtggctgcg cgtcgtcctg ctcgagctgg
     4741 agcgtctcta caaccacgtc ggggacgtgg gcatgatcgt caacgacacc ggcttcgcct
     4801 tcggccacgc gcactgcttc cgtctccgcg aggcgctgct gcggctcaac gaacggatga
     4861 ccggccaccg cctgctacgc ggcgccgtcg tgcccggtgg cgtcaccggc cccgtcgaga
     4921 gggccgacct cggcgacctc gcgagcgagg tcgggcgcct cgtggaggag ttcgggggca
     4981 tcgcgggcct ctgtctcgac aacaccatgg tgctcgagcg gctgcagggc accggtcggc
     5041 tcaccgagcg cacggcccgc gagatgcagg tcgtcggcct cgcgggtcgt gccagcggga
     5101 tcgacgccga cgcccggcgc gacgcgccgt ccgcggcgta cggtcacctc gacgtgcgcg
     5161 tcgccgtcca cgacgccggc gacgtgtggg cgcggacgat ggtgcgcctc cacgaggcgc
     5221 gcgagtccgc ccgcctgctc gcgctggcgg cggagcgtgc gcccgcgggg ccggcacgcg
     5281 cctccctgcc gccgctcccg gccggcggcc acgcgacggg ggtcgtggag gcctggcgcg
     5341 gacccgtgtg gtactgggtg caggccgaag ggccggaccg gctcgcgcgc gtgaaggcgg
     5401 ccgacccgtc gttcaggaac tggcccgcgc tggagtacgc ggtgctcaag aacatcgtcc
     5461 cggacttccc gctctgcaac aagtccttca acctctccta ctccggcagc gacctctgag
     5521 cgtgaccggg cccccggcgc gccgtgcgcg ctgtggtaac gtgggtgcaa ggccgagcat
     5581 cccgccatga tcgtgcacgt gctcgcggtc gattacgacg gcaccatcgc ggagcgcgga
     5641 agcgtgtcgc ccacgaccgc ggccagcctc gcgcgcgtcc gggcgtcggg ccgcaagtgc
     5701 gtgctcgtca cgggacggat gctgcccgat ctgcgccgcg tgtgccccga ggtcgacgcg
     5761 atgttcgacg ccgtggtcgc ggagaacggg gcactcctgt accttccggc ccggcgcgag
     5821 gtcagggcgc tcgggaacgc gcccgagccg acgctggtcg acgcgctgag ccggcgcggg
     5881 gtccgtcacg acctcggcac gtcgatcgtc gccagtgacg atgagtacgc ggaggccgcg
     5941 ctggccgcca tccgcgagac cggcgtcgag cgctcgctcg tcttcaacaa gggctcgctc
     6001 atgctgctgc cgggcggcgt gaccaagggc acgggcctca cggcggcgct cgagaccatg
     6061 gagctctcgc cgcacaacct cgtcggcatc ggcgacgccg agaacgacca cgccttcctg
     6121 gcgctctgcg agtgcgcggt cgccgtcgcc gacgccgtgc ccgccctccg cgagcgggcc
     6181 gatcacgtca cgagcggcgg ggccggcgcg ggcgtcgcgg agttcgtcga aggccacctg
     6241 ctcgccgacg ccgccgacgt cgtgccgcgg ctggcgcgcc accagatccc gctgggccag
     6301 acgccgggcg gcgaggccgt caccgttccc gcgcacgcca ccggcctgct catcgtgggg
     6361 ccgtcggcga ccggcaagtc cacgctcacc ggcgtcctgg tcgagcgcct gatcgaggcg
     6421 ggacgcgcct tctgcctgct cgaccccgaa ggggaccacg acccgctggt cgagctgccc
     6481 ggcgtcgtgg ccctcggcgg caccgggcgc cagacactgc cgggcgccga ggagctgcgg
     6541 cagctgctcc ggcagccggc gtgccggctc gtgctcaacc tcagcgggct gtcgatgagc
     6601 gacaaggtct cgtacgcgac gcacgcgctc ggcgccgtgg ccgccgtccg cagcgcgagc
     6661 ggcctgcccc actggctcat cgtggacgag gcgcaccaca tcgcgcccgc cgacggctcc
     6721 tccgccgtcg agctgctgcc gcccgccggg gagtcgctcg cgctgatcac gctgtcggcc
     6781 gaccggctgg cgcgggccgc gcggcgggcg atcggcgccg tcgcctcgac cgacgtcgag
     6841 gcattccgtg aggcgctggc gacgctgcgc gccgaccgca tgacgggcgc gcggctgccc
     6901 gacgtgcccg gcggggttct cgagcgcggc gaggcggtgc tcgcgtcgct gactgcgggc
     6961 gcggcccgcg tggcgcgctt cgaggtggcc cgccgccagg tcgagcaccg ccgccacgtg
     7021 cgcaagtacg cggagggcga gctccctccc gaccggagct tctacttccg cggaccccgt
     7081 gacgcgctca acctgcgcgc cgtcaacctc aagcggttct gtgagctcgc cgagggcgtg
     7141 gacgaggcga cctggaccca ccacctgggt cggcgcgagt actcggcgtg gatgcgcgcg
     7201 atgatcaagg atcgcgagct cgccgaggag gtcgccgcgg tcgaggcgag ccccggcgag
     7261 cccgcggagt cgcgccggcg ggtgctggag gcggtgcgtc gccggtactc ggtgtgaccg
     7321 cacgagggcc cggcgtggcc ccgatccccc caaacgctcg gcgcgctcca gcgtagctgg
     7381 ggcgcgcctc gattctccgg ctcgacgggt cagcgcgcgc gcgcgacgaa gcggcgcatg
     7441 tcggcccggg cgcgccggct ctcggcctcg atgacgaagc cgttggcctc gaccgtccgt
     7501 tcggtctcgc ggttcggccg gcagccgccg gcgatgcggg tccagagcgg ctgcacggcg
     7561 tcctggaagc gcgccttcca gcggcgcgtc gatcgcacgt gctcgatcat gcgcagcgtg
     7621 ccgcccggcg ccagcacgcg ccggacctcg gagagcccgc gcgcggggtc gggcacactg
     7681 cagaacacca ggctcgagac gacggtgtcg aagacgccgt cgccgaaggg cagcgcctcg
     7741 gcgctcgcct gcaccagcgg cacgtcgggc gcgcggcgcc gcgcacgccg gagcgactcc
     7801 cgcaccggat cggtgcccac cacgcgcacg ctcgccggga agagcggcag cgtccgcccg
     7861 gtgccgcagc cgagatcgag cgtgcggccc cgcgcgccgt cggcgagcca gcgccgccag
     7921 cggccgagcc ccgtacgctc caggaccgcg cacatcgcgt cgtagatcca cgggacctgc
     7981 tcgacgccgc gcatcaggcg gccgggacgc cgccgccgcc cccctcggcc accgacgccc
     8041 cgggcggcag cccgacggcc gggcccggcc ccgccgccgc cgcgccgcgc tcagccccca
     8101 acgtcgtcga agcgggcgag ggcggcgccg gcccggcgca tctcctcgag cgcccgcgct
     8161 ccgtcgcccg gcgagacgtc cacggcccgc acggcatcct cgaggacgac cacctcgaag
     8221 ccttccttca tcgcgtcgag gacggtggcc ttgacgcagt agtcggtggc gagccccccc
     8281 acgaacaggc ggcccacgcc gcgctcgccg agcgccgccg cgaacggcat gccctgggca
     8341 tcctccgcct ggaagcacga gtacgcgtcg tcgccggggt ccatgccctt ggagacgacc
     8401 tcggcgccag cgggcaggct cagcgccggg tggaactcgg ctcccggcgt gccctgcacg
     8461 cagtgcggcg gccaggcgcc gccctgcgcg gcgaagtgac gggtgcgctc cgggtgccag
     8521 tcgcgcgagg cgaacaccgc ggcgccgctc gccgcgaagc gcgcggcgta gcggttgagc
     8581 acgggcacga cgcggtcgcc ttccgggacc gcgagcgagc cgccgggaca gaagtcgttc
     8641 tggacgtcga cgacgacgag ggcgtcgcgg gaggggtcga tcaccagggt catggcccac
     8701 ctccgatcgc cgcggatcgt accactcctg gtcggggccg tggggcctct gctagaatcc
     8761 ccggcgtttc ctcaggggta tacgggcacg ccagcaagga ggccgtcatg aagcgcctgc
     8821 tcaccctcgt cgccgtggtc gcgctggtgc tcacagcggg attctacgcg atcgcccaac
     8881 agccgtacca gctcacgccg atggacgagg ccaagcgcga ccgcgcggtc tcggtcggcg
     8941 gcggcaagta tcacgtcctg ccgtcgacgc tggagacgac gcagtggggc tggctcgacc
     9001 ccaacgagaa gccgaagctc gtgatcagct ccggcgacac cgtcgcgatc gagaccatga
     9061 tgcatg
//
LOCUS       DATIAZ010000331         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1544146, whole genome shotgun sequence.
ACCESSION   DATIAZ010000331 DATIAZ010000000
VERSION     DATIAZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544146"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..805)
                     /locus_tag="VLF19_11455"
     CDS             complement(290..805)
                     /locus_tag="VLF19_11455"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSE93913.1"
                     /translation="MPPIVTRGVLIDVAGAKGQVPLPAEYEVGPDELRSVLAKQGVDV
                     EPGDAVFIRTGIMSLWGDYGHDKAKLAPHDSAGITLAAAKWLVEEKGAVLLGADTSGL
                     ECTAKCWRQPQDFYPVHVYLLVEQGVYIGEFHHLEELASGKADEFLYMVSVNKVRGAT
                     AGFALRPLAIR"
     gene            complement(849..1214)
                     /locus_tag="VLF19_11460"
     CDS             complement(849..1214)
                     /locus_tag="VLF19_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSE93914.1"
                     /translation="MPIVKGFSALRDLPEEKVTDKIGRRVLAGDKEMVVWWSMKAGAY
                     AAAHQHPHEQVFWVLSGRMDIRIGDDKRACVAGALGVIPGGVEHEAWFPEDTEVVDVF
                     APRREDFLTGEAPAYMRQS"
     gene            1490..>2275
                     /locus_tag="VLF19_11465"
     CDS             1490..>2275
                     /locus_tag="VLF19_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE93915.1"
                     /translation="MARLKIGFIPIEGGHYYQEALEEVTRAEDLGFDSVWMEEHHAVT
                     DHYWPSPLTVLAGFATRTSRVMLGTDIVVAAFHHPVRLAEDVAMLDVMSAGRVTLGIA
                     IGYKPDEFALYGVDLEKRGARFEEQLAIMKSLWTRDRVQFRGAFYTVDGRIEPKPLTK
                     PHPPLWIGGWGDLTLKRAATLGDNWIPGPTADLGRLLAGKKRFLENRRAAGRTEPISE
                     WPLTRDLIIADTDRRARELAEEHIMVAYRKEYAGGWRHPFIDAS"
BASE COUNT          368 a          840 c          735 g          332 t
ORIGIN      
        1 cgaacagggc gcccttgacg acctcgggca ggttcacgag cgcgaagaca gggccgtcga
       61 ggctcgtgaa gtacggcgcc agggcggcgc gctcctccgg cgtgaacggc tccgtcatcc
      121 agccgcgata ttacgcccga acgcccccgg gtcccagccc actcagccgg acaccgcgcg
      181 gagggtcgcg acgatcgcca cgccgacggc cacacacacg agcaggttca cgcgcagcga
      241 ggcgagcacg aacaccaccc ggggcgccgg ccaacgtcat gcgagagcgt taccggatcg
      301 ccagcggccg cagcgcgaac ccggcggtcg ccccgcgaac cttgttgaca ctgaccatgt
      361 acaggaactc gtcggccttg ccgctcgcca gctcctcgag gtggtggaac tccccgatgt
      421 acacgccctg ctcgacgagc aggtacacgt ggacaggata gaagtcctgg ggctgcctcc
      481 agcatttcgc cgtgcactcg agccccgagg tgtcggcgcc gaggagcacg gcccccttct
      541 cctcgacgag ccacttcgcc gccgccagtg tgatgcccgc cgagtcgtgg ggtgcgagct
      601 tcgccttgtc gtggccgtag tcgccccaca gggacatgat ccccgtgcgg atgaacacgg
      661 cgtcgcctgg ctcgacgtcc acgccctgct tcgccagcac ggaccggagc tcgtcggggc
      721 cgacctcgta ctcggccggc agcggcacct ggcccttcgc tccggcaacg tcgatcaaca
      781 caccgcgagt gacgatgggc ggcatcctgt cggcgcaggt cttccggggg ccgaagttgc
      841 cgccccactc agctctggcg catgtacgcc ggggcctcgc ccgtcaggaa gtcctcgcgc
      901 cgaggcgcga acacgtccac gacctccgtg tcctcgggga accacgcctc gtgctccacg
      961 ccccccggga tgacgcccag cgcgccggcg acgcacgcac gcttgtcgtc cccgatccgg
     1021 atgtccattc gccccgacag cacccagaac acctgctcgt gtggatgctg gtgcgcggcc
     1081 gcgtacgcgc cggccttcat cgaccaccag accaccatct ccttgtcgcc ggcgaggacg
     1141 cggcggccga tcttgtcggt gaccttctcc tcggggagat cccgcaacgc gctgaaaccc
     1201 tttacgatcg gcatggacgc ctcctggatt gtgaacatgg gggccccgaa atggccccca
     1261 atccccatcg ctcggcactc cccggcaaag cgccggggcg cgatcccctt ccggcgagca
     1321 cccatggagc ccgagatggc cccccacttc aacgctggca cctccgtgct gcaccgggcg
     1381 tcccgacggc tcggcgcgtg gcgctcaccg taatcagcgc ccccggggct gtcaaggatt
     1441 ccgacgacgg cggcggggta caatgccgcg cgccgcgcaa ggagacgcca tggctcgact
     1501 gaagatcggc ttcattccca tcgagggcgg ccactactac caggaggcgc tcgaggaggt
     1561 cacgcgcgcc gaggacctcg gcttcgactc ggtgtggatg gaggagcatc acgccgtcac
     1621 ggatcactac tggccctcgc cgctcacggt cctggcgggc ttcgccactc gcacgtccag
     1681 ggtgatgctc ggcaccgaca tcgtcgtcgc ggctttccac cacccggtcc ggctcgccga
     1741 ggacgtggcg atgctggacg tcatgtcggc cggccgcgtc acgctcggca tcgccatcgg
     1801 ttacaagccc gacgagttcg cgctctacgg tgtcgatctc gagaagcgtg gcgcgcgctt
     1861 cgaggagcaa ctcgcgatca tgaagagcct ctggacgcgg gaccgcgtgc agtttcgcgg
     1921 ggccttctac acggtggacg gccggatcga gccgaagccc ctgacaaagc cgcacccgcc
     1981 gctgtggatc ggcggctggg gcgacctgac gctcaagcgc gccgccaccc tcggcgacaa
     2041 ctggatcccc ggtcccacgg ccgatcttgg ccgcctgctg gcggggaaga agcgattcct
     2101 cgagaatcgc cgcgcggctg gacggacgga gccaatctcc gagtggcccc tcacgcgcga
     2161 cctgatcatc gcggacaccg accggagggc gcgcgagctg gccgaggagc acatcatggt
     2221 cgcctaccgc aaagagtacg cgggcggctg gcgtcatccg ttcatcgacg cctcg
//
LOCUS       DATIAZ010000332         8897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1281057, whole genome shotgun sequence.
ACCESSION   DATIAZ010000332 DATIAZ010000000
VERSION     DATIAZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281057"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..458
                     /locus_tag="VLF19_11470"
     CDS             33..458
                     /locus_tag="VLF19_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSE93916.1"
                     /translation="MLVDFLREDLGLTGTHVGCEHGICGACTILLDGEAARSCIMLAV
                     QADGARITTVEGLMQDGQLHPLQQAFHEHHGLQCGFCTPGMLMTALDFLQAHPEPSEA
                     EIREGIAAVLCRCTGYHGIIEAVKAAARRPGAEASRRQG"
     gene            458..1780
                     /locus_tag="VLF19_11475"
     CDS             458..1780
                     /locus_tag="VLF19_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93917.1"
                     /translation="MSRYLEPDVETAPRDRLRALQEERLRAQVHHAYGASPFYRSKLD
                     AAGTKPERVRTLDDLDRLPFTTKDELKQDQADHPLWGTLLAVPLEQCLRVHMTSATTG
                     RPLAFLDTKDDWYGFYHSYARSLWAFGVRPADMVMAAFSYGPWIGYWSGFHAAQDVGA
                     LVYPAGGLSTDQRIDALRTYPITVLGCTPSYALFLAEQAAQKGIDLARETKVRITWHT
                     GEPGASIPATKARIEAAFGAQAFDLPGLTEIAAWGFECDARAGLTHVHEDYCYPEVLD
                     EQDRPVRPGERGELVFTSLYRRAMPLLRYRTRDVVQLADRACPCGRTLVAFEGGVLAR
                     LDDMKKIRGIIVYPRRIEEIVRAHASVDEFQIVLRRVEGLDDILVRVDPAPALSTDER
                     GRMCLALADALRVGLGIRVTVEATEPGGLPRWDHKARRVKDERTEVPF"
     gene            1780..4119
                     /locus_tag="VLF19_11480"
     CDS             1780..4119
                     /locus_tag="VLF19_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003586076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE93918.1"
                     /translation="MIGERIPRVEDARFLTGQGRYVADLEPAGTLHVAFLRSPHAHAR
                     LISVDTARALAMPGVVACVAGDELADSVRAVRAPSRMRDYRATDHPPIARGKVRHAGE
                     AVAAVVAESRYIAEDAVEALEPGYAVLPVVGDPEAAVRPGAPLVHEEVGTNVLLSRTF
                     TRGDVAAAFGGAAAVVGGRFRFHRHAGVALENRACLAEWRSGDLTLWSSTQVPGIVRD
                     ALAEILSVRAHRIRVVAPDVGGGFGLKTALYPEEIAVCALAMRLGRPVKWVGDRREDL
                     LTSTQAWDEIVDAELAVDAHGAILGLRARVVADIGAYSIYPWTASIEVIQVVSFLPGP
                     YRVEHYAAEALGVATNKAPMGPYRGVGRPVSVFVTEALLDRAARRLHRDPVAVRRENL
                     VRPEELPYRSASGIVWDSGAFVESLDLACETAGYAALRAEQARGRAAGRALGIGVACY
                     VELTGVGSAIPVSPGADVATGTEGATVRVDPAGGVSAIFGLACQGQGHETTLAQVVAA
                     ELGVPVERVRVLHGDTALSPHSTGTYASRSAVLGGGAAILAARAVRDKALAIAAHRLE
                     AAAADLEWRDGAVRVRGAPDRSATLAELAGLAYAGRKRLPEGLEPGLEATRFYDPYFG
                     TASSATHLTLVEVDVATAAVRILRYVVAEDCGRIINPLVVEGQTTGGVVQGAGAALLE
                     EVVYDGEGQLLTASLMDYLVPTAVEAPRVEIVHIERPSPTTLGGFKGVGEGGTIGAPA
                     AVACAVADALAPLGVEVGELPITGERLYRLLAQGKEAPA"
     gene            4665..6542
                     /gene="treZ"
                     /locus_tag="VLF19_11485"
     CDS             4665..6542
                     /gene="treZ"
                     /locus_tag="VLF19_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HSE93919.1"
                     /translation="MKTTSARPRRLPVGADVWPGAGHFRVWAPRAARVEVVIESPRPH
                     AVDLDREGGYFVGEIADIDDGALYRYRLDGRPALLPDPASRFQPQGPHGPSRVVDPSR
                     YRWTDAGWRGPTLDGQVLYEMHVGTFTAEGTWAAAARELPALARLGVTVVEVMPVAEF
                     PGRFGWGYDGVALYAPTRLYGEPDDFRRFVDSAHAAGLAVILDVVYNHLGPDGGYHRQ
                     FSADYHTDRYDNEWGAAWNFDGPNAGPVREFIVANAGYWIDEYHLDGLRLDATQSIHD
                     ASPEHVLAAVVRRVRETGRGRRTLIVAENEPQQAHLVRPADRGGVGIDALWNDDFHHA
                     VRVAATGRKEAYYTDYLGAAQELVSAVKWGFLYQGQRYRWQKKRRGTPALDIPPAALV
                     CYLQNHDQVANSGRGERLHRLTSPGRFRALTALLLLAPQTPLLFQGQEFCASAPWLFF
                     ADHAPGLARAVSRGRDQFLAQFPSLGTAETRAGLPEPGDPATFERCKLDLGERERHAG
                     AHALHGDLLRLRREDPVFAGRHRKHLDGAVLAPEAFVIRFFGGAGDRLLLVNLGRDLA
                     LDPAPEPLLAPPPDAGWMLVWSSEDPRYGGCGTPPPETDEGWRLPGHAAVVLGSETPR
                     R"
     gene            6539..8584
                     /locus_tag="VLF19_11490"
     CDS             6539..8584
                     /locus_tag="VLF19_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSE93920.1"
                     /translation="MIEAPVRRMPWPGARAVEWEPLLTREWLLGNGLGGYAAGSVSGI
                     ITRRYHGLLIAALPPPAGRTVMLSHVWERLRLADRTVVVLTDEENAGGPLTLHGARHL
                     TEFRLETGLPVWRYEVDGYVLEKRVVLLHRQNTVHVTYTLAAGPGSVRLTLRPSLQFR
                     PHDAPVGASRGGPCAITAVEDRYEIAPGSDLPPVRLTVVGRPSAFTIDGKVTRDVLYR
                     VERARGYEHTGDLWSPGYFRLDLEPGASATVVASIESWDVIRSLSPEAARRAEEERRL
                     RLLAAADPAARTAPAAELVLAADQFIVTPPGRMEDMTRARAAGEEARSVIAGYHWFTD
                     WGRDTMISLEGLLLATGRTKEAEWVLHTFAHYVRDGLIPNFFPEGDDEGVYHTADATL
                     WFFHAIGRYLAATDDRLTLGFLLPRLREIVARHLEGTRFGIAVDPRDGLLRQGAEGYP
                     LTWMDAKVGDRVVTPRRGKAVEVNALWYNALRLMEGWERDSGDEGTAKQVGEHAARVY
                     ESFNRRFWYADGGHLYDVVEGEDGDDPACRPNQVLAISLDHPVLDPARWSAVLDVVAA
                     GLLAPVGLRSLAPGHPDYKPRYDGDLRARDAAYHQGTVWGWLIGPFVDAWLRVRPHDR
                     AGARRALEGFVPHLGEACLGSISEIFDADPPFTPRGCVAQAWSVAEVLRCWVKTAER"
     gene            complement(8588..>8897)
                     /locus_tag="VLF19_11495"
     CDS             complement(8588..>8897)
                     /locus_tag="VLF19_11495"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HSE93921.1"
                     /translation="EGKFLWPGYRDNMRVLKWMVERIRGRADGAEETPIGWVPAPSAL
                     DTSGLDMPAARLREALRVDAGEWLAALDDLGQFYDQFGPRLPEPIAKTLAETRRRFGA
                     "
BASE COUNT         1138 a         3231 c         3281 g         1247 t
ORIGIN      
        1 cgctccggga ggggcggtgc gagccgcgca agctgctggt ggacttcctt cgcgaggacc
       61 tcggtctgac ggggacgcac gtcggctgcg agcacgggat ctgcggggcc tgcacgatcc
      121 tcctggacgg cgaggcggcg cgctcgtgca tcatgctcgc agtccaggcg gacggcgcgc
      181 ggatcacgac ggtcgagggg ctcatgcagg atggtcagct tcacccgctg cagcaggcgt
      241 tccacgagca tcacgggctg cagtgcggct tctgcacgcc cggcatgctg atgacggcgc
      301 tcgacttcct ccaggcgcat ccggagccca gcgaggcgga gatccgggag gggatcgcgg
      361 ccgtgctgtg ccggtgcacc ggctatcacg gcatcatcga ggccgtgaaa gccgccgccc
      421 ggcggccggg cgcggaggcg tcgcgacggc agggctgatg tcgcgttacc tcgagcctga
      481 cgtcgagacg gcgccgcgtg atcggctgcg cgcgctccag gaggagcgtc tgcgggccca
      541 ggtccaccac gcgtacgggg cgagcccgtt ctaccggagc aagctcgacg ccgcggggac
      601 gaagcccgag cgggtcagga cgctcgacga cctcgaccgc ctgcccttca cgaccaagga
      661 cgagctcaag caggaccagg cggaccaccc gctctgggga acgctgctcg ccgtgcccct
      721 cgagcagtgc ctgcgggtgc acatgacctc ggcgacgacg ggtcgcccgc tcgccttcct
      781 cgacacgaag gacgactggt acgggttcta ccactcctac gcgcgctcgc tctgggcgtt
      841 cggcgtgcga ccggccgaca tggtgatggc ggcgttctcg tacgggccgt ggatcggcta
      901 ctggtcgggg ttccacgccg cgcaggacgt gggcgcgctc gtgtatccgg cgggcgggct
      961 ctcgacggac cagcgcatcg acgccctccg gacctatccg atcaccgtgc tgggctgcac
     1021 gccctcctac gcgctgttcc tcgccgagca ggcggcgcag aagggcatcg acctcgcccg
     1081 ggagacgaag gtccgcatca cgtggcacac cggcgagccc ggcgcttcga tccccgccac
     1141 caaggcgagg atcgaggcgg ccttcggagc gcaggccttc gacctgcccg gcctcacgga
     1201 gatcgccgcc tggggcttcg agtgcgacgc gcgggcgggg ctgacgcacg tgcacgagga
     1261 ctactgctac cccgaggtgc tcgacgagca ggatcgcccg gtgaggcccg gcgagcgtgg
     1321 cgagctggtg ttcacgagcc tctaccgccg ggcgatgccg ctgctccgct accgcacgcg
     1381 cgacgtcgtc cagctcgccg accgggcgtg cccgtgcggc cggacgctcg tggccttcga
     1441 ggggggcgtg ctggcccgcc tggacgacat gaagaagatc cgcggcatca tcgtctaccc
     1501 gcggcgcatc gaggagatcg tgcgggcgca cgcgagcgtg gacgagttcc agatcgtcct
     1561 tcggcgcgtg gagggcctcg acgacatcct cgtccgcgtc gatccggccc cggcgctctc
     1621 cacggacgag cgcgggcgca tgtgcctggc cctcgccgac gcgctgcgtg tgggcctcgg
     1681 catccgcgtg accgtcgagg ccaccgagcc gggcggcctg ccgcgctggg atcacaaggc
     1741 ccgccgcgtg aaggacgagc gcaccgaagt gccgttctag tgatcggtga gaggattcct
     1801 cgggtcgaag acgcgaggtt tctcaccggg cagggtcgct atgtcgccga tctcgagccg
     1861 gccggcacgc tgcacgtcgc ctttcttcgc agcccgcacg ctcacgcgcg actgatctcc
     1921 gtcgacacgg cgcgcgccct cgccatgccg ggtgtggtgg cgtgtgtcgc gggcgacgag
     1981 ctggcggaca gcgtgcgcgc ggtgagggcg ccgtcgcgca tgcgcgacta ccgggccacc
     2041 gaccacccgc cgatcgcccg cggcaaggtg cgccacgcgg gcgaggccgt ggccgccgtc
     2101 gtcgccgaga gccgctacat cgccgaggac gccgtcgagg ccctcgagcc cggctacgcg
     2161 gtcctgcccg tcgtcggcga ccccgaggcc gcggtccgcc cgggcgcgcc gctcgtccac
     2221 gaggaggtcg ggacgaacgt gctgctgtca cgcaccttca cgcgcggtga cgtggcggcg
     2281 gccttcggcg gcgccgccgc ggtcgtgggc ggccgctttc gcttccatcg tcatgccggc
     2341 gtcgccctcg agaaccgcgc gtgcctcgcg gagtggcgct cgggcgatct caccctctgg
     2401 tcgtccaccc aggtgccagg catcgtgcgc gacgccctcg ccgagattct gtcggtgcgg
     2461 gcgcaccgga tccgcgtcgt cgcgccggac gtgggtggcg gctttggtct gaagaccgcg
     2521 ctctatccgg aggagatcgc ggtctgcgcg ctggcgatgc ggctcggccg gcccgtgaaa
     2581 tgggtcggtg accgccgcga ggacctcctg accagcactc aggcgtggga cgagatcgtc
     2641 gacgccgagc tggccgtgga cgcgcacggc gccatcctgg gcctgagggc tcgggtcgtc
     2701 gccgacatcg gcgcgtactc gatctatccg tggacggcgt ccatcgaggt gatccaggtg
     2761 gtgtcgttcc tgcccggccc gtaccgggtg gagcactacg ccgccgaagc gctgggcgtg
     2821 gccaccaaca aggccccgat ggggccgtac cgcggggtcg gccgcccggt ctcggtcttc
     2881 gtgaccgagg cgctgctcga tcgtgcggcg cgcaggcttc accgtgaccc cgtcgcggtc
     2941 cggcgcgaga acctcgtccg gcccgaggag ctgccgtacc gctcggcgtc cgggatcgtg
     3001 tgggactccg gcgccttcgt ggagtcgctc gacctcgcct gcgagacggc tggctacgcg
     3061 gcgctgcggg ccgagcaggc gcgcgggcgc gccgccggtc gagccctcgg catcggcgtg
     3121 gcctgctacg tcgagctgac cggcgtgggc tcggcgatcc ccgtgtcgcc gggcgccgac
     3181 gtcgcgacgg gcacggaggg cgcgacggtc cgcgtcgacc ccgccggcgg cgtcagcgcg
     3241 atcttcggcc tggcctgtca gggccagggc cacgagacga cgctggcgca ggtcgtggcc
     3301 gccgagctcg gtgtgccggt ggagcgggtc cgggtgctcc acggcgacac ggcactgagc
     3361 ccgcacagca cgggcaccta cgccagccgt agcgcggtgc tgggcggcgg cgcggcgatc
     3421 ctcgccgcgc gcgccgtgcg cgacaaggcg ctggcgatcg ccgcccaccg gctggaggcc
     3481 gccgccgcgg acctggagtg gcgcgacggc gccgtccgcg tgcgcggcgc gccggaccga
     3541 tcggccacgc tcgccgagct cgccggcctg gcgtacgcgg ggcgcaagcg gctgccggag
     3601 ggcctggagc ccgggctcga ggcgacgcgc ttctacgacc cgtacttcgg caccgcctcg
     3661 agcgcgacgc acctgacgct cgtcgaggtg gacgtcgcga cggccgcggt ccggatcctc
     3721 cggtacgtcg tcgccgagga ctgcgggcgc atcatcaatc cgctggtcgt cgaggggcag
     3781 accaccggcg gcgtggtgca gggcgccggc gcggcgctcc tcgaggaggt ggtctacgac
     3841 ggcgagggac agctcctgac ggcgagcctg atggactacc tcgtgcccac ggcggtcgag
     3901 gcgccgcgcg tggagatcgt gcacatcgag cggccgtcgc cgacgaccct cggcggcttc
     3961 aagggcgtcg gagagggcgg caccatcggc gcgccggcgg ccgtcgcttg tgccgtcgcc
     4021 gacgccctgg ccccgctggg cgtcgaggtc ggcgagttgc cgatcacggg agagcggctg
     4081 taccgtctgc tggcgcaggg gaaggaagcc ccagcatgag caaccgggcg ttcgtgacgg
     4141 cggcgctgct ctgcctagcg gcgacaacgc ccgtacgatc tggtcctcat cgcttcaccg
     4201 gctatccgtt gcaagctggc cacaccatga tgtcggcctc ggccgcctga gagctcgccg
     4261 ctgacaccgc gcaggcgcgg cccgccgctg ggaacccatg cccatcatcg ctgatctcac
     4321 ccagctcgct cgggacctgg tcgccactct tggtccttac ctccagcggt gggacgttcc
     4381 cgacacgaaa agcgggtcgc accactagtc taggcggcgg tcaccgcggc tcgcgcagtc
     4441 cgccggcgac caatcggact ccgaggcccg cccggcgctc ggccatcccc gatctcctgg
     4501 ccggcaagga cgcgccgcac gtgttggcag cccggccagg ccgctcggaa gcgccgtggc
     4561 ggtcggtacc tgctaccgat tcgcgcggat gcctgtcccc gcggagatgg ccgcggccgc
     4621 gccccgacgg caccgaacgt gcacttcggc aggccgagag agcgatgaag acgaccagtg
     4681 cccgcccgcg gcgtctgccc gtcggggccg atgtctggcc aggcgccggg cacttccgcg
     4741 tgtgggcgcc gcgcgccgca cgcgtggagg tggtgatcga gtccccgcgt ccgcatgcgg
     4801 tcgatctgga ccgcgagggc ggctacttcg tcggggagat cgcggatatc gacgacggcg
     4861 cgctctaccg ttaccggctc gacggccgtc ccgcgctgct tcccgacccg gcgtcgcgct
     4921 tccagcccca gggtccgcac ggcccgtcgc gcgtcgtcga cccgtcccgc taccggtgga
     4981 ccgatgccgg ctggcgcggg ccgacgctcg atggccaggt gctctacgag atgcatgtgg
     5041 gcacgttcac cgccgagggc acgtgggcgg cggcggcccg cgagctgccc gcgctggcgc
     5101 ggctcggtgt caccgtcgtg gaggtcatgc cggtcgcgga gtttcccggg cgattcggct
     5161 ggggctacga cggcgtcgcg ctctacgcgc cgacgcgact ctacggcgag cccgacgatt
     5221 tccgtcgctt cgtggacagc gcgcacgcgg ccggcctcgc ggtcatcctc gacgtggtct
     5281 acaaccatct cggcccggat ggtggctatc accgccagtt ctccgccgac taccacaccg
     5341 atcgctacga caacgagtgg ggcgcggcgt ggaacttcga cgggcccaac gccggtcccg
     5401 tgcgcgagtt catcgtcgcc aacgccggct actggatcga cgagtaccat ctggacggcc
     5461 tgcgcctgga cgccacccag tcgatccacg acgcctcgcc cgagcacgtg ctggccgccg
     5521 tcgtgcggcg ggtccgcgag accgggcggg gccggcgcac gctgatcgtc gccgagaacg
     5581 agccgcagca ggcccacctg gtccgcccgg ccgacagggg cggagtcggg atcgacgccc
     5641 tctggaacga cgacttccac cacgcggtgc gggtcgccgc gacgggacga aaggaggcct
     5701 actacaccga ctatctcggc gccgcccagg agctcgtctc cgcggtgaag tggggcttcc
     5761 tctatcaggg gcagcgctac cgctggcaga agaagcgccg cggcacgccg gccctcgaca
     5821 tcccgcccgc ggcgctggtc tgctacctcc agaaccacga ccaggtggcg aactccgggc
     5881 gcggggagcg gctgcatcgg ctcacgagcc ccgggcgctt ccgggcgctg acggccctgt
     5941 tactgctcgc gccccagacg ccgctgctct tccaggggca ggagttctgc gcgtccgcgc
     6001 cgtggctgtt cttcgccgac cacgcgcccg gcctcgcccg ggccgtctca cgcggccgtg
     6061 accagttcct cgcacagttc ccgagcctcg gcacggcgga gacgcgtgcg ggcctgcccg
     6121 agcccggcga ccccgcgacg ttcgagcgct gcaagctcga cctcggcgag cgcgagcgcc
     6181 acgccggggc ccacgcgctc catggcgacc tgctgcggct gcgccgtgag gaccccgtgt
     6241 tcgcgggccg ccaccggaag catctcgacg gtgcggtact cgcgcccgag gcgttcgtca
     6301 tccgcttctt cggcggcgcc ggtgaccggc tgctgctcgt caacctcggg cgcgatctcg
     6361 cgctggaccc ggcgcccgag ccgttgctgg cgccgccccc ggacgcgggc tggatgctcg
     6421 tgtggtcgag cgaggacccg cgctacgggg gctgcggcac gccgccgccg gagaccgacg
     6481 agggctggcg gctgcccggg cacgctgccg tcgtcctggg ctcggagacg ccgcggcgat
     6541 gatcgaggcg ccggtcaggc gcatgccatg gccgggcgcg cgggcggtgg agtgggagcc
     6601 gctgctcacg cgcgagtggc tgctgggcaa cgggctcggc ggctacgccg ccggctcggt
     6661 gtcgggcatc atcacgcgcc gctaccacgg gctgctcatc gcggcgctgc cgccgcccgc
     6721 cggacgcacc gtgatgctct cccacgtctg ggagcggctg cggctggccg accgcaccgt
     6781 cgtcgtgctg accgacgagg agaacgcggg cggcccgctg acgctccacg gcgcgcgcca
     6841 tctcaccgag ttccggctgg agaccggtct gccggtgtgg cgctacgagg tcgacggcta
     6901 cgtcctcgag aagcgcgtgg tgctgctgca ccggcagaac accgtgcacg tcacgtacac
     6961 gctcgccgcg gggccgggca gcgtccgcct cacgctgcgg ccgtccctgc agttccgtcc
     7021 gcacgacgcc ccggtcggtg cgtcacgagg cggtccgtgc gcgatcaccg cggtcgagga
     7081 ccggtacgag atcgccccgg ggtccgacct gccgccggtg cggctcaccg tcgtcggccg
     7141 cccgagcgcg ttcaccatcg acggcaaggt cacgcgcgac gtgctgtacc gcgtggagcg
     7201 cgcccgaggg tacgagcaca cgggcgacct gtggagcccg ggatacttcc ggctggacct
     7261 cgagcccggc gcctcggcca cggtcgtcgc gtcgatcgag tcgtgggacg tgatccgctc
     7321 gctctcgccc gaggcggcgc ggcgcgcgga agaggagcga cgtctccggc tgctcgcggc
     7381 ggccgacccg gccgcacgca cggcgccggc ggcggagctg gtgctcgctg ccgaccagtt
     7441 catcgtcacg ccgccggggc gcatggagga catgacccgc gcccgcgccg ccggggagga
     7501 agcgcggagt gtgatcgctg gctaccactg gttcaccgac tgggggcgtg acacgatgat
     7561 cagcctcgaa gggctgctcc tcgccaccgg ccgcaccaag gaggccgagt gggtgctgca
     7621 caccttcgcc cactacgtgc gggacgggct gatccccaac ttcttccccg agggcgatga
     7681 cgaaggcgtc tatcacaccg cggacgcgac gctctggttc ttccacgcga tcgggcgcta
     7741 cctcgcggcg acggacgacc gactgacgtt ggggttcctc ctgccgaggc tgcgggagat
     7801 cgtcgcccgg cacctcgagg gcacgcgctt cggcatcgcg gtcgatccgc gcgacggcct
     7861 gctgcgccag ggggcggagg gttacccgct cacgtggatg gacgcgaagg tcggcgaccg
     7921 ggtcgtgacg ccgcggcggg gcaaggccgt ggaggtcaat gccctctggt acaacgccct
     7981 ccgtctgatg gagggatggg aacgcgattc cggcgacgag gggacggcga agcaggtcgg
     8041 cgagcacgcc gcgcgcgtct acgagtcgtt caaccgccgc ttctggtacg cggacggcgg
     8101 tcacctctac gacgtcgtcg agggcgagga cggcgacgac ccggcgtgcc gtcccaacca
     8161 ggtcctcgcc atcagcctcg atcaccccgt gctcgatccc gcgcgctgga gcgcggtgct
     8221 cgacgtcgtc gccgcggggt tgctggcacc ggtggggctg cgttcgctgg cgcccgggca
     8281 tcccgactac aagccgcgct acgacggcga cctgcgggcc cgcgacgccg cataccacca
     8341 gggcacggtg tgggggtggc tcatcggacc gttcgtggat gcgtggctgc gggtgcgccc
     8401 gcacgaccgg gccggcgcgc gccgcgcgct cgagggcttc gtgccgcacc tcggcgaggc
     8461 ctgcctcggg tcgatcagcg agatcttcga cgcggacccg cccttcacgc cgcgcggctg
     8521 tgtcgcccag gcgtggagcg tggccgaggt gctgcgttgc tgggtgaaga cggccgagcg
     8581 gtaggcctca ggctccgaag cggcggcggg tctccgccag cgtcttcgcg atcggctcgg
     8641 gcaggcgggg cccgaactgg tcgtagaact ggccgaggtc gtccagcgcg gcaagccact
     8701 cgccggcgtc cacccggagc gcctcgcgca gccgcgccgc cggcatgtcg agccccgacg
     8761 tgtcgagcgc gctcggcgcc gggacccacc cgatgggcgt ctcctcggcg ccgtcggccc
     8821 gcccgcggat gcgctcgacc atccacttga gcacgcgcat gttgtcgcgg tagcccggcc
     8881 agaggaactt gccttcg
//
LOCUS       DATIAZ010000333         2187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1545786, whole genome shotgun sequence.
ACCESSION   DATIAZ010000333 DATIAZ010000000
VERSION     DATIAZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545786"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="VLF19_11500"
     CDS             complement(<1..1670)
                     /locus_tag="VLF19_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE93922.1"
                     /translation="MSVTVGVSVRRREDARLLVGAGRYLDDLQVPGMLHLAIVRSPHP
                     HARIVSVERRAARACPGAVAVFTVDDLPELGGSVPPLVPAPAIRAYAHPVLAGPVVRH
                     AGEAVVVVAAEDRYAAADAAERVRVEYEPLPAATSPAVALATGAPRVHADWPDNVAGR
                     NASVVGDPDRALVEADVVVDADVVYPRMAGMPIETRGVLAFVDPLSGVLEVWTSTQVP
                     FAVRSAIAAILGIPEDGVRVRAPEVGGGFGVKGHDYPEDVLVPAVARALGRPVKWTET
                     RAEHLLAAAGDRDQTHHARLGLRRDGTIVALETRFTRDHGAYPTLGEAITLNTINHLP
                     GPYRVPAYRATADNVVTHKTFLAAYRGAGRPEAALVLDRLLDRAARTLGMDPAELRRR
                     NLIRADEMPYRTGLSYRDGVPISYDPADYVAGFDRLLERLDYAGWRQRQRERRGTARP
                     LGIGLSAYVEGTGLGPFEGADVRVDPGGTVFVRVGVSAQGQAHETTLAQICAERLGVP
                     LDQVVVLGGDTDLVGFGMGTIASRVAAVTGPAVARSADEVAHRARLVAA"
     gene            1788..>2187
                     /locus_tag="VLF19_11505"
     CDS             1788..>2187
                     /locus_tag="VLF19_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSE93923.1"
                     /translation="MAALTAIFLLLLAPVAASGADLPIFDAHVHYSHDAWDVLSPKEA
                     VALLRKGGVKRALVSSSNDEGTQKLHAEAPDVVIPELRPYRRRGEIGTWVRDESVITY
                     LEERLRKHRYVAIGEFHLSGADAELPVVRRM"
BASE COUNT          295 a          793 c          826 g          273 t
ORIGIN      
        1 gcggccacga gcctcgcgcg gtgcgcgacc tcgtcggcgc tccgggccac ggcgggtccc
       61 gtcaccgcgg ccacgcggct ggcgatcgtt cccatgccga agccgaccag gtcggtgtcg
      121 ccgccgagca cgacgacctg atcgagcggc actcccagac gctcggcgca gatctgggcg
      181 agcgtggtct cgtgcgcctg gccctgggcg gagacgccga cccgcacgaa aacggtgccg
      241 cccgggtcca cgcgcacgtc ggcgccctcg aacggcccga ggccggtgcc ttcgacgtac
      301 gccgagaggc cgatgccgag cggccgcgcg gtgccgcggc gctcccgctg ccgctggcgc
      361 cagccggcgt agtcgagccg ctcgagcagc cggtcgaagc cggccacgta gtcggcgggg
      421 tcgtaagaga tcggcacgcc gtcgcggtac gagagccccg tgcggtaggg catctcgtcc
      481 gcccggatca agttgcgccg cctcagctcc gccgggtcca tcccgagcgt ccgcgcggcg
      541 cgatcgagca ggcggtcgag cacgagcgcc gcctcgggcc gcccggcgcc acggtaggcg
      601 gcgaggaagg tcttgtgggt gacgacgttg tcggcggtcg cgcggtaggc cggcacgcga
      661 tagggcccgg gcaggtgatt gatcgtgttc agcgtgatgg cctcgccgag cgtcggatag
      721 gcgccgtgat cgcgggtgaa gcgcgtctcc agcgcgacga tcgtgccgtc gcgccgcaga
      781 ccgaggcgcg cgtggtgggt ctggtcgcgg tcgcccgccg cggccagcag gtgctccgcg
      841 cgcgtctcgg tccacttgac ggggcggccg agcgcccggg ccacggcggg gacgaggacg
      901 tcctcggggt agtcgtggcc cttgacgccg aagccgccgc cgacctcggg cgcccgcacg
      961 cgcacgccgt cttcggggat gccgaggatc gcggcgatgg cgctgcgcac ggcgaacggg
     1021 acctgggtgg aggtccacac ctcgaggacc ccgctgagcg gatcgacgaa ggccaggact
     1081 ccgcgcgtct cgatcggcat gccggccatc cgcggataga ccacgtcggc gtcgacgacg
     1141 acgtcggcct cgacgagcgc gcggtcgggg tcgccgacga cggaggcatt gcggccggcg
     1201 acgttgtcgg gccagtcggc gtgcacgcgg ggagcgccag tcgcgagcgc caccgccggc
     1261 gatgtggccg ccggcagcgg ctcgtactcg acgcgcacgc gctcggcggc gtcggcggcc
     1321 gcgtagcgat cttcggcggc gacgacgacc acggcctcgc ccgcgtgacg gacgacggga
     1381 cccgccagga cgggatgcgc gtacgcccgg atcgccggcg ccggcaccag cgggggaacc
     1441 gacccgccca gctcgggcag atcgtcgacg gtgaacacgg cgacggcgcc cgggcacgcg
     1501 cgcgccgcgc gccgttcgac ggacacgatg cgtgcatgag ggtgcgggct cctcacgatg
     1561 gcgaggtgga gcatcccggg cacctggagg tcgtcgagat accggccggc gcccacgagg
     1621 aggcgcgcgt cctccctgcg cctgacggaa acgccgaccg tcacggacac gtggccacgg
     1681 ccgtcagcgg gcggccaggc gaggggcctc gtagaggatc ctcggtacca tcgcgtgtat
     1741 tctagtcgcc ggccggattc ccggcacgct cggtggaggt gcgacagatg gccgcactga
     1801 ccgcgatctt cctgctgctc ctcgccccgg tcgccgcgtc gggggcggat ctgccgatct
     1861 tcgacgccca cgtccactac agccacgacg cctgggacgt gctgtcgccg aaggaggccg
     1921 tcgcgctgct ccgcaagggc ggcgtcaagc gcgcgctcgt gtccagctcg aacgacgaag
     1981 gcacccagaa gctccacgcg gaggcccccg acgtcgtcat ccccgagctg cgtccctacc
     2041 gccgccgggg cgagatcggc acgtgggttc gcgacgagtc cgtcatcacc tacctcgagg
     2101 agcgcctgcg caagcaccgc tacgtcgcca tcggtgagtt tcacctctcc ggcgccgacg
     2161 ccgagctgcc ggtcgtgcgc cgcatgg
//
LOCUS       DATIAZ010000334         6362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_627482, whole genome shotgun sequence.
ACCESSION   DATIAZ010000334 DATIAZ010000000
VERSION     DATIAZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627482"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..878
                     /locus_tag="VLF19_11510"
     CDS             504..878
                     /locus_tag="VLF19_11510"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HSE93924.1"
                     /translation="MSKRTSKPKKRRATRKARARRAGEAPERGEIVDAVEERASHPEA
                     SPVLSGGDVDADWQGALSSGEEAVGGSVATPDQDVVDEIGRALGVEQAPDAELHMSEE
                     ILRARTRRYWALEHRAAERPEP"
     gene            875..1330
                     /locus_tag="VLF19_11515"
     CDS             875..1330
                     /locus_tag="VLF19_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE93925.1"
                     /translation="MSAGFARILVPTDFSAPSEEAWKTACRLGRVLGAELILVHVFVE
                     PPLYSEGPFSGERVREVYAAGRAWVEKTLEEWAAGARAEGLAARVLVRVGVPHREILA
                     LAAYERADLLVVGTHGRGGVERALIGSVADRVIRLAPCPVLSVRGPAEA"
     gene            1327..3441
                     /locus_tag="VLF19_11520"
     CDS             1327..3441
                     /locus_tag="VLF19_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HSE93926.1"
                     /translation="MTPPLAPFFEPATVAVVGASRDPAKVGGSVLANLTAAGFSGRVV
                     PVNPRADRVQGLPTVASVRAIDGDVDLAVVVVPAADVLGVLKDCVAKGVRGAVVISAG
                     FRESDDEGRTRETELRTWLRDQPLRVIGPNCLGWIRPTRGLNLTFAPGMPAPGRVAFV
                     SHSGALAVAMLDWSRNHHLGFSLFASLGNQADVTETDVLRAVAADPETGVVIAYLEGV
                     ADGRAFVGALSQVAALKPVVVLKTGRSREGARAVSSHTGALAGSDTAFDAALRQAGVV
                     RARSIEELFDLARALETQPLPSGRRLLVVSNGGGLGIVATDAAAAAGLAVTSLPEDAR
                     ARLRAVLPRTASVVNPVDIVGDADAARFGNALRAATAAGVDAALVLLTAQAATDAGEV
                     ARAIVSATRGWTIPVAGAFVGGPRVAPGAATLEQAGIPCYPFPERAVAALAGMAAVVE
                     RRAVRQAPAPPGVPLEAAVRHVAALRRAEARRLGILELAPLLAAYGIPHAAPRAVATP
                     EAAAAAAAEMGTPVALKLVSPDIVHKTEIGGVRLGLDSPAAVADAMRAMLARVAAARP
                     AARLRGALLQPMVAPGRELLLGALRDPQFGPMVVVGAGGIWVEVFADTAARLAPVDRE
                     EAIVMLGELRMAPLLLGTRGERSVDFGALADLVSRFSRLAVDLGDFVELEVNPLVAGP
                     GGAVAVDARATLDAAPTAVSDG"
     gene            3479..4348
                     /locus_tag="VLF19_11525"
     CDS             3479..4348
                     /locus_tag="VLF19_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE93927.1"
                     /translation="MAKEFRVVVATDGSPVARAAVAAAVEFPWPANSQASGVLARAPL
                     PATADLTPTVWAAIGQSLERVAAEARRTLRRRWPDAEVAVLDKLPADAILAEARGAQA
                     IVLGSRGHGVVGRLLLGSVSRQVVRSATCPTLVVKGSARGFQHFVIGVDGSANSRRAA
                     AFVALLERPRGARVTLVAVVEPVRVGSMGLMPAGIRATLGREMRAMSAERVRRAQRQL
                     HAAARPLARAGWRVRTAVRTGLPLTELTAAAEAGDVLVVGARGVGGVERLLLGSIAEG
                     VLTRSQVPVVIVR"
     gene            4376..>6362
                     /locus_tag="VLF19_11530"
     CDS             4376..>6362
                     /locus_tag="VLF19_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSE93928.1"
                     /translation="MTGRSVPHERVPRWPDDASGLLAGDAADRLRRYGPNAIVESPPR
                     PWADVTRDTARDPMIWFLAAAAALYALVGQIAEALTLLAAVVPLVGMDAFLHRRTRAS
                     TAGLAGRLATRARVVRDGAVVEVPSLDVVPGDLVVVGAGEPVPADGLVVAGEDLKADE
                     STLTGEAYPVAKRPLTIEMETPTRPPRARRSARPGEAVARLDVPQGVGAEVEEMPVAG
                     EHWMLAGTRLLTGRSSVRVVFTGAETLYGEIVRSATGARARTPLQHAIERLVSVLIGA
                     AGVVCLALAAVRLTQGHGWVDALVSAVTLASAALPEEFPVVFTLFLAVGVYRLARRQA
                     LVRRPVSVETIGRVTTICTDKTGTLTEGRLVLTHLLPAAGAGEARLLALGAAACRADG
                     GDPLDAALLGEAARRGVVAPGRVRERVPFTEARAREAVLLEEGAETLWVVKGAPERVL
                     AAARLAGTERDAWSERVAALAASGHKVIACAWRPHSGPPPAEPEDDYQLAGLLAFEDP
                     VRKGVAAAVAECREAEIHTMMVTGDHPLTAGAVARELGLGGGAPVVVSGGEIDALTAG
                     GETAALRRIDVVARAVPAQKLALVRALQAAGEVVAVTGDGVNDVPALQAADVGIAMGE
                     RGTRPAREVAAIVLLDDDFGTIVRAIAEGRQLFRNLKM"
BASE COUNT          723 a         2323 c         2486 g          830 t
ORIGIN      
        1 cggcgcacga agggggtgcg gggctgggag cgccgggacc gtcgagggag gagttgctgg
       61 cgcgggccgc gcagcccggc gaggaggcgt tgcggctcca cgcgctctgc ggcggcaaga
      121 tgcagaccct cccgaagtgc cagatccggg gccgcgacga cttctcgatc taacgccggt
      181 catccgggag gggctgatcc cgatcccgtc gtcggagagg tccgagctct tccggttcgg
      241 cagccccagg tagcgcaagg cgcggcgggg cgagccgacc ccagtgggac cacgcctccc
      301 cgcaccagtc cgacgcgtgg cggcggccga gcgcagaacc tgccgtcgtc gttgacgttc
      361 gaccatgccc gttgtccggc acccaggatg catggaacgc cccgcggaag ggacgcgctg
      421 gcaaagctgc ccaaggctct gaaggatgtc cgggccgaag cctgggcgcg aggtggaccg
      481 agtgttcccg aggaggcgcg atcgtgagca agcggacgag caagcccaag aagaggcgcg
      541 cgacgcggaa agcccgcgcc cggcgcgcgg gcgaggcacc ggagcgcggc gagatcgtcg
      601 acgcggtcga ggagcgcgcg agccatcccg aggcgagccc cgtactgagc ggtggtgacg
      661 tcgacgcgga ctggcagggc gcactgagct ccggcgagga ggccgtgggc ggcagcgtcg
      721 ccacgcccga tcaggacgtc gtggacgaga tcggccgcgc gctcggggtc gagcaggccc
      781 ccgacgcgga gctgcacatg tccgaggaga tcctgcgcgc ccggacgagg cgctactggg
      841 cgctcgagca tcgcgccgcc gagcggccgg agccgtgagc gccggcttcg cccgcatcct
      901 cgtgccgacc gacttctccg cgccgtcgga ggaggcgtgg aagacggcct gccggctggg
      961 ccgcgtcctc ggcgcggagc tgatcctcgt gcacgtgttc gtcgagccgc cgctctattc
     1021 ggagggcccc ttcagcggcg agcgcgtgcg cgaggtctac gcggccggcc gggcctgggt
     1081 cgagaagacg ctcgaggagt gggcggccgg ggcgcgcgcc gagggactgg ccgcgcgcgt
     1141 gctcgtgcgc gtcggcgtgc cccaccggga gatcctggcg ctcgcggcct acgagcgtgc
     1201 ggacctcctc gtcgtcggca cccacggtcg cggaggggtc gagcgcgccc tgatcgggag
     1261 cgtcgccgac cgcgtgatcc gcctggcgcc gtgtcccgtg ctgtcggtcc gcggacccgc
     1321 ggaggcatga ccccgccgct cgcgcccttc ttcgagccgg cgaccgtcgc cgtcgtgggg
     1381 gcctcgcgcg accccgcgaa ggtcggcggc agcgtgctcg cgaacctcac cgccgccggc
     1441 ttctcggggc gcgtcgttcc ggtgaacccg cgcgccgaca gggtgcaggg cctgcccacg
     1501 gtggcatccg tccgcgccat cgacggcgac gtcgacctcg cggtggtcgt ggtgccggcg
     1561 gccgacgtgc tcggggtgtt gaaggattgc gtcgccaagg gcgtgcgtgg cgccgtcgtc
     1621 atctccgcgg gcttccggga gagcgacgac gagggccgca cgcgcgagac ggagctgcgg
     1681 acgtggctgc gcgatcagcc cctgcgggtc atcggaccga actgcctcgg ctggatccgg
     1741 cccacgcgcg gcctcaacct cacgttcgcg cccggtatgc cggcgccggg ccgcgtcgcc
     1801 ttcgtctcgc actccggagc gctcgcggtg gcgatgctcg actggtcgag gaaccaccac
     1861 ctgggcttct cgctcttcgc gagcctgggg aatcaggcgg acgtcaccga gaccgacgtg
     1921 ctgcgcgccg tggccgccga ccccgagacg ggggtcgtca tcgcctacct cgaaggcgtc
     1981 gccgacggtc gcgccttcgt cggtgcgctg tcgcaggtcg ccgcgctcaa gcccgtcgtc
     2041 gtgctcaaga ccggccggtc ccgggagggc gcgcgtgccg tgtcctcgca caccggcgcg
     2101 ctggcgggct cggacacggc gttcgacgcg gcgctgcggc aggccggcgt cgtccgggcg
     2161 cggtcgatcg aggagctgtt cgacctggcg cgcgcgctcg agacccagcc gctcccgtct
     2221 ggccggcgcc tgctcgtcgt cagcaacggc ggcggcctcg gcatcgtcgc caccgacgcc
     2281 gccgccgcgg ccgggctcgc ggtcacgtcg ctgccggagg acgcgcgtgc ccggctgcgg
     2341 gccgtgctcc cgcgcacggc gagcgtcgtc aaccccgtcg acatcgtcgg ggacgccgac
     2401 gccgcgcgct tcggcaacgc gctgcgcgcg gcaacggcgg ccggcgtgga cgccgcgctg
     2461 gtgctgctca ccgcgcaggc ggcgacggac gcgggcgagg tggcacgcgc catcgtgtcc
     2521 gcgacacgcg gatggaccat tcccgtcgcg ggggcgttcg ttggcggacc ccgcgtggcg
     2581 cccggggcgg cgaccctcga gcaggcgggg atcccgtgct accccttccc ggagcgcgcg
     2641 gtggccgcgc tcgcgggcat ggcggcggtc gtcgagcgtc gtgccgtgcg ccaggcgccg
     2701 gcgccgccag gcgtgccgct cgaagcggcg gtgcgccacg tggcggccct gcggcgcgcg
     2761 gaagcccgcc ggctcgggat cctggagctg gcgccactgc tcgccgccta tggtatcccc
     2821 cacgccgccc cgcgcgcggt ggcgacgccc gaagcggcgg cggccgccgc cgcggagatg
     2881 ggaaccccgg tcgccctcaa gctcgtgtcg ccggacatcg tgcacaagac cgagatcggt
     2941 ggcgtccgtc tcggcctcga ctcgccggcc gcggtcgcgg acgccatgcg ggcgatgctc
     3001 gcccgcgtgg ccgcggcgag accggcagcg cggctgcgcg gcgcgctgct ccagccgatg
     3061 gtcgcgccgg ggcgtgagct gttgctcggt gccctgcgtg atccccagtt cggcccgatg
     3121 gtcgtcgtcg gggcgggggg catctgggtc gaggtgttcg ccgatacggc ggcgcggttg
     3181 gcccccgtgg accgggagga ggcgattgtc atgctcggcg agctacgcat ggcgccgctg
     3241 ttgctcggca cgcgcggcga gcggtcggtg gacttcggag ccctggccga cctcgtcagc
     3301 cgtttctcgc ggctcgcggt agacttgggc gatttcgtgg agctcgaggt gaacccgctg
     3361 gtcgccggcc cgggcggggc ggtcgccgtc gacgcgcgag ccacgctcga cgcggccccg
     3421 accgccgtca gcgacggata gaacgccgcc aacggtcagc gatcgagatg gaggacagat
     3481 ggccaaggaa ttccgggtag tggtcgcgac cgacgggtcc cccgtggccc gggcggccgt
     3541 cgcggccgcg gtcgagtttc cgtggccggc gaactcgcag gcgtccggcg tcctggcgcg
     3601 ggcgccgctg ccggcgaccg cggacctgac gccgacggtg tgggccgcga tcggccagag
     3661 cctcgagcgc gtcgccgccg aggcccggcg gacgttgcgc cggcgctggc cggacgccga
     3721 ggtcgccgtc ctcgacaagc ttcccgccga cgcgatcctg gccgaggcgc ggggcgctca
     3781 ggcgatcgtc ctcggctcgc gcgggcacgg cgtggtcggc cgcctgctgc tcggcagcgt
     3841 ctcgcggcag gtcgtgcgga gcgccacgtg cccgacgctc gtcgtcaagg gcagcgcacg
     3901 agggttccag cacttcgtca tcggcgtcga cggttcggcc aactcgcgtc gcgcggcagc
     3961 gttcgtggct ttgctcgagc gcccgcgggg tgcgcgcgtc acgctggtcg cggtcgtgga
     4021 gcccgtacgc gtcggctcga tggggctcat gccggccggc atccgtgcga ccctcggcag
     4081 ggagatgcgg gcgatgagcg cggagcgggt gcgccgggcg caacgccagc tgcacgccgc
     4141 ggcgcgtccg ctcgcgcgcg cgggctggcg cgtgcgcacg gccgtgcgta ccgggttacc
     4201 gctcacggag ttgaccgctg cggcggaagc cggcgacgtc ctcgtcgtcg gcgcccgcgg
     4261 cgtcggcggc gtcgagcggc tgctgctcgg cagcatcgcc gagggcgtcc tcacccggag
     4321 ccaagtcccg gtcgtcatcg tccgctgagc ccgacggcgc gccgggcgcc ccgcgatgac
     4381 tggccgatcg gtaccgcacg agcgagttcc ccgctggccc gacgacgcgt ccggcctgct
     4441 cgcgggggac gcggccgatc gcctgcggcg ctacggcccc aacgcgatcg tcgagtctcc
     4501 tccgcgcccg tgggcggacg tcacgcgcga cacggccagg gacccgatga tctggttcct
     4561 cgccgccgcc gccgcgctct acgcgctcgt gggccagatc gccgaggcgc tcaccctgct
     4621 ggccgcggtc gtacccctcg tcggcatgga cgcttttctc caccggcgca cgcgcgcgtc
     4681 gaccgcgggc ctcgcgggcc ggctcgccac gcgcgcgagg gtggtgcgcg acggcgccgt
     4741 cgtcgaggtg ccgagcctcg acgtcgtccc cggcgacctc gtggtcgtcg gtgcgggcga
     4801 gcccgtcccg gccgacggcc tcgtcgtcgc cggcgaagat ctgaaggccg acgagtcgac
     4861 gctgacgggc gaggcctatc ccgtcgcgaa gcgtccgttg accatcgaga tggagacccc
     4921 gacacggccc ccacgcgccc gacgctcggc ccgccccggc gaggccgtgg cgcgcctcga
     4981 tgtcccgcaa ggtgtgggag cggaggtcga ggagatgccg gtggcgggcg agcactggat
     5041 gctcgccggc acgcggctgc tcaccggccg atcctccgtc cgcgtggtct tcacgggcgc
     5101 cgagacgctc tacggggaga tcgtgcgctc tgccaccggc gctcgcgcgc gcacgccgct
     5161 ccagcacgcg atcgagcgtc tcgtctcggt gctgatcggc gccgccggcg tggtgtgcct
     5221 ggcactcgcg gcggtgcgcc tgacgcaggg tcacggctgg gtggacgcgc tcgtcagcgc
     5281 cgtgaccctg gcgtcggccg cgctgcccga ggagttcccc gtcgtgttca cgctcttcct
     5341 cgccgtcggc gtctaccggc tggcccggcg tcaggcgctg gtccggcggc ccgtctccgt
     5401 cgagaccatc ggacgcgtga cgacgatctg caccgacaag acgggaaccc tcaccgaggg
     5461 ccggctggtg ctcacgcacc tgctgcccgc cgccggcgcc ggcgaggcac gcctgctcgc
     5521 gctgggcgcg gcggcgtgcc gggccgacgg cggcgatccg ctcgacgccg cgctgctggg
     5581 cgaagccgcc cgccgtggcg tggtcgcgcc gggccgggtg cgcgaacgcg ttcccttcac
     5641 cgaagcgcgt gcgcgcgagg cggtgctcct cgaggagggc gccgagacgc tgtgggtcgt
     5701 caagggtgcg cccgagcggg tgctcgccgc cgcgcggctc gcgggaaccg agcgcgacgc
     5761 ctggagcgag cgagtcgcgg cgctggccgc ctcgggccac aaggtcatcg cgtgcgcctg
     5821 gcggccgcac agcgggccac cgccggccga gcccgaggac gactatcagc tcgccggcct
     5881 gctggcgttc gaggacccgg tgcgcaaggg cgtcgccgcg gcggtcgcag agtgccgcga
     5941 ggccgagatt cacacgatga tggtcaccgg cgaccatccg ctgacggcgg gcgcggtggc
     6001 gcgcgagctc gggctcggtg gcggcgcgcc ggtggtcgtc tcgggcggcg agatcgacgc
     6061 gctgaccgcc ggcggggaga cggccgccct ccggcgcatc gacgtggtcg cgcgcgcggt
     6121 ccccgcccag aagctcgccc tggtgcgcgc gctgcaggcc gccggcgagg tcgtcgccgt
     6181 caccggcgac ggcgtgaacg acgtgcccgc gctgcaggcc gcggacgtcg ggatcgccat
     6241 gggcgagcgc gggacgcggc cggcgcgcga ggtggcggcc atcgtgctcc tcgacgacga
     6301 cttcggcacg atcgtgcgcg cgatcgcgga aggccgccag ctcttccgga acctcaagat
     6361 ga
//
LOCUS       DATIAZ010000335         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_102029, whole genome shotgun sequence.
ACCESSION   DATIAZ010000335 DATIAZ010000000
VERSION     DATIAZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102029"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1152)
                     /locus_tag="VLF19_11535"
     CDS             complement(256..1152)
                     /locus_tag="VLF19_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSE93929.1"
                     /translation="MSTRALDALDRARTVVVLAVSPLEEHGPHLPLGVDVFTAEYFAR
                     EIAQRLTAERPGWSAVLAPTLHLGAFTFDAPGTVRVRQRVVRDALVDYGRAFARAGFR
                     FVLVANGHAGPTHLAALDEAAAIVSRRYGVHMASLSGWFAWQFRTGRFLDGVEAQLGR
                     ALSDTERRAFAEDAHAGWWETSMMLMLRPDLVGSFRDLPPATYSLPERLVPNYPLRDG
                     GAGYVGHPALADPAFAKATAEALLEEVMALVHALLDGRVAPGERRSPFYALPFFRTDF
                     WPAVAAGGLALAAVVWLVPRRR"
     gene            complement(1173..2594)
                     /locus_tag="VLF19_11540"
     CDS             complement(1173..2594)
                     /locus_tag="VLF19_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSE93930.1"
                     /translation="MSVAQAKAKPAPARPGRLYGAVRQVARFWLWFLFKRVDVRHPER
                     VPPTGPVLLCINHPNNLIDSLLVGAVLERKVHYLATAALFRNPLLARFLRACGAIPVY
                     RKQDGVSTGAARATGNADAFAACHAAFDDGALVAIYPEGTTHAEARVQRIKTGAARIA
                     LGYEADHPGALTVLPVGLSFEARKSFRGRVLVAFGEPVGVAPYLTPWRSDAVAAVDAL
                     TTTIQSAMEGQVVHVDRIDNETLVRAVEELYRDELVRQIREERGLAERQIDVFRLSRT
                     IAAAADHFRKHEPERLESLWQRIRGYRAMLAAYRVKDEAVRARLARGPARRRVVWTGE
                     AIAGLPLFAYGAVVNALPYFIPRWIARATARKETDYATTRLLATVVAVPLFWGLETWL
                     VARAADPAWALVFLLSLPVSGLIAYHYLRGIGALRRDVRFGMLALTRDTAARELVAER
                     AAIIADLDRAKTDYLAATRGSSF"
     gene            complement(2591..>3017)
                     /locus_tag="VLF19_11545"
     CDS             complement(2591..>3017)
                     /locus_tag="VLF19_11545"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HSE93931.1"
                     /translation="LAARLEAIRVEAAERMGVRGSDAVPKIAVVAPPAPFRGLDRRDY
                     AAGDADVLARVISMGTCHRAFALTSAMCLGVAARLAGTVVHECLADDGRSPDIRLAHP
                     SGVLPIDATVATRDGQPWAERVTVYRTARRLMEGYVRVP"
BASE COUNT          438 a         1072 c         1154 g          353 t
ORIGIN      
        1 gcagcggtga gcgcggtgtt cagcgcgttg agggcttgcg gacgattgag ccacagccat
       61 cccacctcgc cctggcgctc gaagtccagc atcagcgcca cgcgctcccg ccgggccgat
      121 cgcggccgtc caccagcgcg gcgcgatctc gtccggatga tcgacgtgga gcatcggcgc
      181 cgcgccactc cccggagcgc ctgccgccgg ccatcggcgc gggcaccgcc tcgccggagc
      241 gctcgaggcg gcacatcacc gacgacgggg gacgagccag accactgcgg ccagcgccag
      301 cccgccggcg gcgacggcgg gccagaagtc agtgcggaag aacggcagcg cgtagaaggg
      361 cgagcgacgc tcgccggggg ccacgcgccc gtccaacagc gcgtggacga gcgccatgac
      421 ctcttcgagc agcgcctcgg ccgtggcctt tgcgaaggca ggatcggcca gcgcagggtg
      481 gccgacgtag ccggcgccgc cgtcgcgcag cgggtagttg ggcaccaggc gctcgggcag
      541 cgaatacgtc gccggcggca ggtcgcggaa gctgcccacc aggtccggcc ggagcatcag
      601 catcatcgac gtctcccacc agccggcgtg ggcgtcctcg gcaaaggcgc gacgctcggt
      661 gtcggagagg gcgcggccga gctgggcctc gacgccgtcg aggaagcggc ccgtgcggaa
      721 ctgccaggcg aaccagccgc tgagcgacgc catgtgcacg ccgtaccgcc gcgagacgat
      781 cgccgccgcc tcgtcgaggg cggcgaggtg cgtgggcccg gcgtggccgt tggcgaccag
      841 gacgaagcgg aatccggcgc gcgcgaaggc gcgcccgtag tccaccagcg cgtcacgcac
      901 cacgcgttgc cgcacccgca cggttcccgg cgcgtcgaag gtgaacgcgc ccaggtgcag
      961 cgtgggcgcc agcaccgccg accagcccgg ccgctccgcc gtcagccgct gggcgatctc
     1021 gcgggcgaag tactcggcgg tgaagacgtc cacgccgagc ggcaggtggg gcccgtgctc
     1081 ctcgagcggg ctcaccgcga ggacgacgac ggtgcgggcg cggtcgagcg cgtcgagggc
     1141 acgcgtggac atctcttcca gtcggtggat gctcaaaagg aggacccccg ggtggcggcg
     1201 aggtagtcgg tcttcgcgcg gtccagatcg gcgatgatgg cggcgcgctc ggccacgagc
     1261 tcgcgcgccg ccgtgtcgcg cgtgagcgcg agcatgccga agcgcacgtc gcggcgcagc
     1321 gcgccgatgc cgcgcagata gtggtaggcg atgaggcccg agaccggcag cgagagcagg
     1381 aacaccagcg cccaggcggg atccgcggcc cgcgcgacga gccaggtctc gaggccccag
     1441 aagagcggca ccgccaccac ggtggccagc agccgcgtgg tggcgtagtc ggtctccttg
     1501 cgggccgtag cgcgggcgat ccagcgcggg atgaagtagg gcagggcgtt gacgacggcg
     1561 ccgtaggcga agagcggcag gccggcgatg gcctcgccgg tccacacgac tctccgccgc
     1621 gccgggccgc gggccagtcg cgcccgcacg gcctcgtcct tcacccggta ggccgccagc
     1681 atggcgcggt agccgcggat gcgctgccag aggctctcga ggcgctcggg ctcgtgcttg
     1741 cggaagtggt cggcggcggc ggcgatggtc cgggagagcc ggaacacgtc gatctgccgc
     1801 tcggccagtc cgcgttcctc gcggatctgg cgcaccagct cgtcccggta cagctcctcc
     1861 accgcgcgga cgagcgtctc gttgtcgatc cggtccacgt ggacgacctg gccctccatg
     1921 gccgactgga tcgtcgtggt cagcgcgtcc acggcggcga ccgcgtcgct gcgccacggc
     1981 gtcaggtacg gcgccacgcc cacgggctcg ccgaaggcca ccagcacgcg cccgcggaac
     2041 gacttgcgcg cctcgaacga gagccccacc ggtagcacgg tgagcgcgcc ggggtggtcc
     2101 gcctcgtaac ccagcgcgat gcgtgcggcg ccggtcttga tgcgctgcac ccgcgcctcg
     2161 gcgtgcgtgg tgccctcggg gtagatggcg accagcgcgc catcatcgaa cgcggcgtga
     2221 caggcggcga aggcgtcggc gtttccagtc gcgcgtgcgg cccccgtgct cacgccgtcc
     2281 tgcttccggt agacgggaat ggcgccgcag gcacgcagga agcgcgcgag cagggggttg
     2341 cggaacagcg cagccgtggc gaggtagtgg accttgcgct cgagaacggc gccgaccagc
     2401 agggaatcga tcaggttgtt ggggtggttg atgcagagca ggacggggcc cgtcggcggc
     2461 acgcgctcgg ggtggcgcac gtccacgcgc ttgaagagaa accagagcca gaagcgggcg
     2521 acctggcgga ccgcgccgta gagccgtccg gggcgtgcgg gcgcgggctt cgccttggcc
     2581 tgggcgacgc tcacggcacg cggacgtagc cttccatgag ccgtcgcgcc gtgcggtaca
     2641 ccgtcacgcg ctcggcccac ggctgtccgt cgcgcgtcgc taccgtcgcg tcgatcggca
     2701 gcacgccgga gggatgcgcg agcctgatgt cgggggaccg gccgtcgtcg gccaggcact
     2761 cgtggacgac ggtgccggcc aggcgcgcgg ccacgcccag gcacatcgcc gacgtgagcg
     2821 cgaaggcgcg atggcaggtg cccatcgaga tcacccgcgc gagcacgtcg gcgtcgcccg
     2881 cggcgtagtc gcgccggtcc agcccgcgga acggcgccgg gggcgcgacg acggcgatct
     2941 tcggcacggc gtcgctgcca cgaacgccca tacgctcggc cgcttccacg cggatcgcct
     3001 cgagccgcgc cgcgagg
//
LOCUS       DATIAZ010000336         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1153139, whole genome shotgun sequence.
ACCESSION   DATIAZ010000336 DATIAZ010000000
VERSION     DATIAZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153139"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VLF19_11550"
     CDS             <1..585
                     /locus_tag="VLF19_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSE93932.1"
                     /translation="IVKRREGTWELLILVGVLGLAVAGMVATAWVQTDGPDSAAASAA
                     VEPTVETATQARAAVRAPATPSVLHADLYFDFKSTRLRADAVRVLQENVALMDASDGW
                     VVLVQGYADRHGPADYNRALAQRRAEAVKQFLAELGVPEISIRVASVGQDGALCDDPG
                     AECQQLNRRVHVEMRKLPGAGAADTTPVATGQAR"
     gene            582..>2458
                     /locus_tag="VLF19_11555"
     CDS             582..>2458
                     /locus_tag="VLF19_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HSE93933.1"
                     /translation="MRLLLFVLVTVMAVPASPARSLGGDATVVASRATEGALWWRPAP
                     DEPPSPAPLLATDVDMKVTGVVLRAVVRQEFVNPGATWVEGRYVFPLPDDGAVDHLRM
                     RVGDRVIEGVVQERAGAKTTYQRAKQQGRRVSLVEQERPNVFTASVANVPPGASVAIE
                     IEYQQVLRLDDGQVRLRFPMVVGPRYIPGTPLTTQHGPDSDGSGRAQDTDEVPDASRV
                     TPRVRHPARGAINPVSLRIELRPGAPLARLEAPHHRVATAPLEGGGVLVTLADGAVPA
                     DRDFELVWELTRGTVPTVSVGAESTDRGVFALLMVVPPAAAEGPRTRREVVFVIDTSG
                     SMHGASISQATAALKLALARLDSADTFNVIQFNSVTDSLFASAQPATAVNRFLADRYV
                     SALRAQGGTEMLPALSLALDAQEHRGRLRQVIFLTDGAVGNEERLFAAIGERLGDSRL
                     FTVGIGSAPNSHFMREAARIGRGAFTYVGSATEVQAKMEALFRKLESPVLTDVRLELP
                     GATPTEMYPARIPDLYLGEPILVALRADAMPARAVLRGRLGPERWEQEVWLQDADAGA
                     GLGVHWARAKIAALLDRKWSGASPDDVRTAVVEVALAHHLVSPYTSLVAVDVTPARPG
                     DA"
BASE COUNT          331 a          871 c          925 g          331 t
ORIGIN      
        1 atcgtgaaga ggcgcgaggg cacctgggag ctgctgatcc tggtgggcgt cctcggcctg
       61 gccgtcgcgg ggatggtcgc gacggcgtgg gtgcagacgg acgggccgga ctccgccgcg
      121 gcgagcgccg ccgtcgagcc cacggtggag actgccacgc aggctcgcgc ggcggtccgg
      181 gcgccggcca cgccctcggt cctgcacgcc gacctctact tcgacttcaa gagcacccgc
      241 ctccgggcgg atgccgttcg cgtcctccag gagaacgtgg ccctgatgga cgcgtccgac
      301 gggtgggtgg tgctggtcca ggggtacgcc gaccgccacg gaccggcgga ctacaatcgc
      361 gctctcgccc agcgccgtgc cgaggcggtc aagcagttcc tggccgagct gggcgtcccg
      421 gagatctcca tcagggtggc gagcgtgggg caggatggcg ccctgtgcga cgaccccggc
      481 gcggagtgcc agcagctcaa ccggcgagtc catgtcgaga tgaggaagct gccgggcgcc
      541 ggcgccgcgg acacgacgcc ggtcgccacc gggcaggctc gatgaggctc ctgcttttcg
      601 tcctcgtcac cgtgatggcc gtgccggcga gccccgcccg aagcctgggc ggggacgcca
      661 ccgtcgtggc gagtcgagcg accgagggcg cgctctggtg gcggcccgcc ccggacgagc
      721 cgccgtcgcc ggcgccgctg ctcgccaccg acgtcgacat gaaggtgacc ggtgtcgtcc
      781 ttcgcgcggt ggtgcgtcag gagttcgtca acccgggtgc gacgtgggtc gaggggcgtt
      841 acgtgttccc cctgccggat gacggcgcgg tggaccacct gcgcatgcgc gtgggcgacc
      901 gcgtgatcga gggcgtcgtc caggagcgcg ccggcgcgaa gacgacctac cagcgggcga
      961 aacagcaggg acggcgggtc agtctcgtcg agcaggagcg tcccaacgtg ttcaccgcgt
     1021 cggtcgccaa cgtcccgccg ggcgcgtcgg tcgccatcga gatcgagtac cagcaggtgc
     1081 tgcggctcga cgacggacag gtccggctgc gcttcccgat ggtcgtcggc ccgcgctaca
     1141 tccccggtac gccgctcacg acgcagcacg gcccggacag cgacggttcg ggacgggcac
     1201 aggacaccga cgaggtgccg gatgcatcac gagtgacgcc gcgtgtccgg catccggctc
     1261 gcggcgccat caatccggtg agcctcagga tcgagctgag gcccggcgcg ccgctcgcgc
     1321 ggctggaggc gccccaccat cgcgtcgcga cggcgccgct ggagggcggt ggcgtcctcg
     1381 tcacgctcgc cgatggcgcc gtccccgccg accgggactt cgagctggta tgggagctga
     1441 cgcgagggac ggtgcccacg gtcagcgtcg gggccgagtc cacggaccgg ggcgtgttcg
     1501 cgctcctcat ggtggtcccg ccggcggccg cggaggggcc gcggacacgg cgcgaggtcg
     1561 tcttcgtcat cgacacctcg ggctcgatgc acggggcgtc gatctcgcag gcgacggccg
     1621 cgctgaagct cgcgctggcc cgcctggact ccgccgacac gttcaacgtc atccagttca
     1681 acagcgtgac cgacagcctg ttcgcgagcg cccagccggc gacggcagtc aaccgcttcc
     1741 tggccgaccg ttacgtgagc gcgctcaggg cccagggcgg cacggagatg ctgccggcgc
     1801 tctcgctggc gctcgacgcg caggagcatc ggggacggct acgccaggtg atcttcctga
     1861 ccgatggcgc cgtcggcaac gaggagcgac tcttcgccgc gatcggggag cgcctcggcg
     1921 acagccggct cttcacggtc gggatcgggt cggcgccgaa cagccacttc atgcgcgagg
     1981 cggcgcggat cggacgcggc gccttcacgt acgtcggcag cgccaccgag gtgcaggcga
     2041 agatggaggc gctcttccgc aagctggagt cccctgtgct gaccgacgtt cggctcgagc
     2101 tgcccggcgc gacgccgacg gagatgtacc ccgccaggat ccccgacctc tacctcggcg
     2161 agccgatcct cgtggcgctg cgcgccgacg ccatgcccgc ccgcgccgtg ctgcggggtc
     2221 gcctcgggcc ggagcggtgg gagcaggagg tctggctcca ggacgccgac gccggtgccg
     2281 gcctcggggt gcactgggcc cgggccaaga tcgcggccct gctggaccgg aagtggtccg
     2341 gagcgagccc cgacgacgtc cggaccgcgg tcgtcgaggt ggcgctcgcg caccatctgg
     2401 tgagcccgta cacgagcctg gtcgccgtcg acgtcacgcc ggcgcgcccc ggcgacgc
//
LOCUS       DATIAZ010000337         3228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_102297, whole genome shotgun sequence.
ACCESSION   DATIAZ010000337 DATIAZ010000000
VERSION     DATIAZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102297"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VLF19_11560"
     CDS             <1..463
                     /locus_tag="VLF19_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93934.1"
                     /translation="LAAPILALDTSSFTVTSGSVVVVNNSHLRVTGDLFSLVNGSTLT
                     ATSPSPGQALSGALVRVSNGGVLNVSGGLVNFGGTGGNTINLTNGLCPSGDCFTFTGG
                     IRVHFANNGTSSNVQIGGTPIKNPALGQFNIPNDVHIIVDGPASQVIISGK"
     gene            492..887
                     /locus_tag="VLF19_11565"
     CDS             492..887
                     /locus_tag="VLF19_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93935.1"
                     /translation="MGQVALVVALVGMLALVPGSAVAQTGAQRPPAAAPGTAPPATPG
                     PQPEIKLDETTSLKASALESRMAAIVANYALLQRQAQDLQQEMKTVLEERKKLIEEAA
                     RRGRVEVGDANEWAYENKGQRYVKIKRTP"
     gene            complement(912..2495)
                     /locus_tag="VLF19_11570"
     CDS             complement(912..2495)
                     /locus_tag="VLF19_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93936.1"
                     /translation="MARSLAVVLAGLLLLDAFTVPALAQTTEADVYVGQAIVDFDDKR
                     YDAALENLKRALEIEPDHVEALYYTGVVYMVQRRPAAAVPFLERARAKSPKEPSLAYQ
                     LGLAYFALEQYDRALPLLEQAFAVQPELDSLGYYLGFLRYRNKNYRGALSALRAGRAS
                     DPEIQQLARVYTGLSLAALGLPRQAEAEVEQALRIAPGSAITGPAERLRDAVVASRDR
                     DRRFTAEVRAGVLYDDNVRVLPGGIPLGSVDGELVAAIRQGSDLPEDSVGELFGVRAE
                     YVWWRTADWESSVGYSFFATLYNDLPDFNITDHLVTATLVHKNAIAAMPLQIGLQYAY
                     DALFLNEDLFIQRNTASLFGVLTESERHLTQLLARYQDKEFNEIGLTLPDESRDANNY
                     MVGFQHFFRFAEDRHFIKVGYQWDRDDAEGKNYVYNGNRFLFGAQATLPWGDVRLRFD
                     FDAHLRDYTHKNTLFPLIEPGTKRRSDQEYNYAFRVEVPLPWNLTLVAEYLRTDANSN
                     LAIFDYDRNVTSLSLSWVY"
     gene            complement(2582..>3228)
                     /locus_tag="VLF19_11575"
     CDS             complement(2582..>3228)
                     /locus_tag="VLF19_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004872323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSE93937.1"
                     /translation="GLLDDPFAFLASLVLKIPALLMAVTVHEVAHGWVADRRGDPTAR
                     RQGRLTLNPLPHIDPLGALAFVLVGFGWAKPVPVNAANLRNPARDMLLVAVAGPLSNF
                     VAAFLGLVAFALVRQAGLPGFLAEPVAGILLWVFQFNLALGIFNLVPLPPLDGGHFLR
                     YFLPQGAAGLLHVLDQVGPLILLVLVVSGGTRYIVEPAFRMLTSVYVAAVRAIL"
BASE COUNT          544 a         1080 c         1153 g          451 t
ORIGIN      
        1 cctcgcggcg ccgatcctgg cgctcgacac gagctcgttc accgtgacca gcggctccgt
       61 ggtcgtcgtg aacaacagcc acctcagggt caccggcgac ctgttctccc tggtcaacgg
      121 cagcacgctg acggcgacgt cgccgtcgcc cggccaggcg ctgagcggcg cgctcgtccg
      181 ggtctcgaac ggcggcgtcc tgaacgtgag tggcgggctc gtcaacttcg gcggcaccgg
      241 gggcaacacg atcaacctga ccaacggtct ctgcccctcg ggcgactgct tcacgttcac
      301 cggcggcatc cgcgtccatt tcgccaacaa cggcacctcg agcaacgtcc agatcggtgg
      361 cacgccgata aagaacccgg cgctcgggca gttcaacatc ccgaacgacg tccacatcat
      421 cgtcgacggc ccggcgtcgc aagtgatcat cagtggcaag tagcccgctc gacagcctgg
      481 aaggagtctc gatgggccag gtggctctcg tcgtcgccct cgtcggcatg ctcgccctcg
      541 ttcccggttc cgccgtcgcg caaaccggcg cccagcggcc ccccgcggcg gcgccgggca
      601 cggccccgcc cgccacccct gggccgcagc ccgagatcaa gctcgacgag accacgagcc
      661 tcaaggcctc cgcgctcgag tcgcgcatgg ccgcgatcgt ggccaactac gcgctgttgc
      721 aacggcaggc ccaggacctg cagcaggaaa tgaagacggt cctcgaggag cgcaagaagc
      781 tcatcgagga ggcggcccgg cgcgggcggg tcgaggtcgg cgacgcgaac gagtgggcct
      841 acgagaacaa ggggcagcgc tacgtgaaga tcaagcggac gccctgaggc gcccggagcc
      901 ccccatggac gtcagtacac ccaggagagc gagagcgacg tcacgttccg gtcgtagtcg
      961 aagatggcca ggttggagtt ggcgtccgtc ctgaggtact ccgccaccag cgtcaggttc
     1021 cagggcagcg gcacctccac ccggaaggcg tagttgtact cctgatcgga gcgccgcttg
     1081 gtcccgggct cgatcaaggg gaacagggtg ttcttgtggg tgtagtcgcg caggtgggcg
     1141 tcgaagtcga agcgcaggcg gacgtccccc cagggcagcg tcgcctgcgc gccgaacagg
     1201 aagcggttgc cgttgtagac gtagttcttg ccctcggcgt cgtcgcggtc ccactggtag
     1261 ccgaccttga tgaagtggcg gtcctcggcg aagcggaaga agtgctggaa ccccaccatg
     1321 tagttgttgg cgtcgcgtga ctcgtcgggg agcgtgagac cgatctcgtt gaactccttg
     1381 tcctggtaac gggcgagcag ctgggtcagg tgccgctcgc tctcggtgag caccccgaag
     1441 agcgacgccg tgttgcgctg gatgaacagg tcctcgttca ggaagagcgc gtcgtacgcg
     1501 tattgcagcc cgatctggag gggcatcgcg gcgatcgcgt tcttgtggac gagcgtggcg
     1561 gtcacgaggt ggtccgtgat gttgaagtcc ggcaggtcgt tgtagagggt cgcgaagaac
     1621 gagtagccga ccgacgactc ccagtccgcg gtgcgccacc agacgtactc ggcgcgcacg
     1681 ccgaagagct cgcccaccga gtcctccggg aggtccgagc cctggcggat cgccgccacg
     1741 agctcgccgt ccacgctgcc gagggggatc cctcccggca gcacgcggac attgtcgtcg
     1801 tacaggaccc cggcccgcac ctcggccgtg aagcgccggt cgcggtcgcg ggaggccacc
     1861 acagcgtcgc gcaggcgctc ggcggggccg gtgatcgccg agccgggcgc gatccgcagc
     1921 gcctgctcga cctcggcctc cgcctgcctc ggcagcccca gcgccgccag cgagagaccc
     1981 gtgtagacgc gggcgagctg ctggatctcg gggtcgctcg cacgcccggc gcgcagggcc
     2041 gagagcgcgc cgcggtaatt cttgttgcgg tagcgcagga agcccaggta gtagccgagg
     2101 ctgtcgagct cgggctggac ggcgaacgcc tgctccagga gcggcagtgc gcggtcgtac
     2161 tgctccaggg cgaagtaggc gaggcccagc tggtacgcca ggctcggttc cttcggcgac
     2221 ttcgcccgcg cgcgctccag gaacgggacc gccgcggccg gccggcgctg aaccatgtac
     2281 acgacgcccg tgtagtagag cgcctcgacg tgatcgggct cgatctcgag cgcacgcttg
     2341 aggttctcga gcgcggcgtc gtagcgcttg tcgtcgaagt cgacgatcgc ctggccgacg
     2401 tacacgtcgg cctcggtcgt ctgcgcgagg gcgggaacgg tgaacgcgtc gagcaggagc
     2461 agtccggcaa ggactaccgc gagcgagcga gccacctgcc ctttatagcc gaagtcttcg
     2521 accgagtgca aatcgtgatg cggtggccct ccgcgaggaa cccgagcggc cggcgccggc
     2581 gtcagaggat ggcgcggacc gcggccacgt agacgctcgt cagcatccgg aacgccggtt
     2641 ccacgatgta ccgggtcccc ccgctcacca cgaggacgag caggatcagg ggaccgacct
     2701 ggtcgaggac gtgcagcaga ccggcggcgc cctggggcag gaagtaccga aggaagtggc
     2761 cgccgtcgag cggcggcagg gggaccaggt tgaagattcc cagcgcgagg ttgaactgga
     2821 agacccagag caggatccct gccacgggct cggccagaaa cccgggcaac ccggcctgcc
     2881 ggacgagcgc gaacgcgacg aggccgagga acgcggcgac gaagttcgag agcggccccg
     2941 cgacggccac gagcagcatg tcccgggccg ggttcctgag gttggcggca ttcaccggaa
     3001 cgggcttcgc ccagccgaag cccacgagca cgaaggccag ggcgccgagc ggatcgatgt
     3061 gcggcagcgg gttcagcgtg agccggcctt gccggcgcgc ggtcgggtcg ccccgccggt
     3121 cggccaccca gccgtgggcc acctcgtgaa cggtgacggc catcagcagc gccgggatct
     3181 tgagcacgag cgaggcgagg aacgcgaacg ggtcgtcgag gaggccgc
//
LOCUS       DATIAZ010000338         2956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_365262, whole genome shotgun sequence.
ACCESSION   DATIAZ010000338 DATIAZ010000000
VERSION     DATIAZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365262"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..943)
                     /locus_tag="VLF19_11580"
     CDS             complement(452..943)
                     /locus_tag="VLF19_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSE93938.1"
                     /translation="MTPTERESSIERYANGPGRLREALATVPVEAMQWRPKAGEWSAH
                     EVVVHCADSETQAASRIRILVSEREPLILGYDQETWARDFDYHAHPVDLALAVVDAVR
                     ANTAALLRRLPERVWQREGRHTESGRYTAEDWLHIYAEHLEGHARQIEANVAAWRRRA
                     GRG"
     gene            complement(1052..2500)
                     /locus_tag="VLF19_11585"
     CDS             complement(1052..2500)
                     /locus_tag="VLF19_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HSE93939.1"
                     /translation="MGKVTGFLEITRKKWPTRAVAERLRDWKEVYLPYPDEDLKKQAA
                     RCMDCGVPFCHQGCPLGNIIPDWNDLVYRNRWREAIDRLHATNNFPEWTGRLCPAPCE
                     GACVLGINDDPVTIKAVEVSIVEHAFDQGWVAPEPPAVRTGKRVAIVGSGPAGLAAAH
                     QLNRAGHWVIVFERADRIGGLLRYGIPEFKMEKRFLDRRLRLMEKEGVVFQTGADVGT
                     SPTMADLRKEFDAVVLACGAGQPRDLPVPGRELAGIHFAMDYLTLQNRRCEGDVVPDD
                     RFVSAKDKHVVIIGGGDTGADCLGTVHRQGAASVHQFELLPRPPDTRAADNPWPLWPN
                     VFRVSPAHEEGGERVYAVSTQRFVGDAQGHVRALEAVKVEMIHEDGRLSFPPVAGSEF
                     TLPVDLVLLALGFLGPDRRGPIDELGITLTERGNVARDAAWMTSVPGVFTCGDMQRGQ
                     SLIVWAIAEGRSCARGVDLYLKGKSDLPAPLP"
     gene            complement(2504..>2956)
                     /locus_tag="VLF19_11590"
     CDS             complement(2504..>2956)
                     /locus_tag="VLF19_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006630922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93940.1"
                     /translation="VAGERFAVRNSGALTVVEGVGDHACEYMTGGRVVVLGRTGRNFA
                     AGMSGGVAYVLDRAGDFARRCNPGMVDLEPLAEAEDINLVRDLIERHVDVTGSTFAAA
                     VLADWPALRPKFVKVMPRDYKRVLAAEARARAEGRQPTFAELVGQTSG"
BASE COUNT          429 a         1062 c         1048 g          417 t
ORIGIN      
        1 aacgacttcg tcatcgggct cgtcgggcgc gacctgcacc cgctctcgca cctggtgcag
       61 acgatgcgga agaccgtgca gacctcgcag tacctccaga ccctcgggcc attcttcgtg
      121 ggccacgtcg tctggcagag caccgcgcgc tgacgcctcg gtggtcaggc ctcggggagc
      181 cgcgtctcga gccaggacag gacttcgggg acgaggcgcg cgagcgtgcg cccgctgagc
      241 gcgaggcccc agtgcgaggc gcccgtgact tcgatctcgg cagcgaaggg aaagtcccag
      301 agctgccgga cgcgcccgga gttggcggcg ccgtggacga agacgatcgg cggccgacgg
      361 acgagggagg atgaggaggg cgtctcgcca cctgcatccc gcccgcgccc ggcgggcagg
      421 agcgcgcgga cagcgagtac cgcgggcggc gctagccgcg gccggcccgc cgccgccagg
      481 cggccacgtt ggcctcgatc tgtcgcgcgt ggccctcgag gtgctcggcg tagatgtgca
      541 gccagtcctc ggccgtgtaa cgaccggact cggtgtggcg cccctcgcgc tgccagacgc
      601 gctcgggcag gcggcgcagc aacgccgcgg tgttggcacg cacggcgtcc acgaccgcga
      661 gcgcgagatc gaccggatga gcgtggtagt cgaaatcacg cgcccaggtc tcctggtcgt
      721 agccgaggat gaggggctcc ctctcggaca cgaggatccg gatgcgcgag gccgcctggg
      781 tctcggaatc agcgcagtgc acgaccacct cgtgcgccga ccactcaccc gccttcggcc
      841 gccactgcat cgcctccacc ggtaccgtcg ccagcgcctc ccgtagtcgc ccggggccat
      901 tcgcataccg ttcgatcgag ctctcgcgct cggtcggtgt catggccatc agtcgacctc
      961 cgttcgcgcg gcggctcttg ccgccgcatc cgacccgagg agacctcgaa aaggacttcg
     1021 aggctcctcc gacgagtgat gggcctccga ttcagggcaa gggagccgga aggtcggact
     1081 tgcccttcag gtacaagtcg accccgcggg cacacgaccg gccctcggcg atcgcccaca
     1141 cgatcagcga ctggccacgc tgcatgtcgc cgcaggtgaa cacacccggc acgctcgtca
     1201 tccaggcggc gtcgcgcgcg acgttgccgc gctccgtcag cgtgatcccc agctcgtcga
     1261 tgggcccccg gcggtcgggc cccaggaagc ccaaggcgag caggacgagg tccacaggca
     1321 gcgtgaactc gcttcccgcg acggggggga agctcagccg tccgtcctcg tggatcatct
     1381 cgaccttcac ggcctcgagc gcgcgcacgt ggccctgcgc gtcgccgacg aagcgctggg
     1441 tggacacggc gtacacacgc tcgccgccct cctcgtgggc cggggagacg cggaagacgt
     1501 tgggccacag gggccagggg ttgtcggccg cacgcgtgtc gggcggccgc gggagcagct
     1561 cgaactggtg gaccgatgcc gctccctgcc ggtgcacggt cccgagacag tcggcgccgg
     1621 tgtcgccgcc accgatgatc accacgtgct tgtccttcgc ggagacgaac cgatcgtcgg
     1681 ggaccacgtc gccctcgcag cgcctgttct gcagcgtcag gtagtccatc gcgaagtgaa
     1741 tcccggcgag ctcccggccc ggcaccggca gatcgcgcgg ctggccggcg ccgcaggcga
     1801 gcacgacggc gtcgaactcc ttgcgaaggt cggccatggt gggactcgtg ccgacgtcgg
     1861 caccggtctg gaagacgacg ccttccttct ccatgaggcg caggcgccgg tccaggaacc
     1921 gcttctccat cttgaactcg gggatcccgt aacgcaggag gccgccgatc cggtccgcgc
     1981 gctcgaacac gatgacccag tggccggccc ggttgagctg gtgcgcggcg gcgagccccg
     2041 ccgggccgga gccgacgatg gccacgcgct tgcccgtgcg cacggccgga ggctccggcg
     2101 cgacccagcc ctggtcgaag gcgtgctcga cgatcgacac ctcgacagcc ttgatggtga
     2161 ccgggtcgtc gttgatgccg agcacgcagg cgccctcgca gggcgccggg cacagccggc
     2221 ccgtccactc cgggaagttg ttggtcgcat gcaggcggtc gatggcctcg cgccatcggt
     2281 tccggtacac gaggtcgttc cagtccggga tgatgttgcc gagcgggcag ccctgatggc
     2341 agaaggggac gccgcagtcc atgcagcgcg ccgcctgctt cttgaggtcc tcgtcgggat
     2401 acgggaggta gacctccttc cagtcgcgga gccgctcggc cacggcccgc gtcggccact
     2461 tcttgcgcgt gatctcgagg aagccggtga ccttgcccac gcgctacccg ctggtctggc
     2521 cgacgagctc cgcgaacgtc ggctggcggc cctccgcccg cgcccgggcc tcggcggcca
     2581 gcacgcgctt gtagtcgcgg ggcatcacct tcacgaactt cggccgcagg gcgggccagt
     2641 cggccaggac ggcagccgcg aacgtggagc ccgtcacgtc gacgtggcgt tcgatcaggt
     2701 cgcgcaccag gttgatgtcc tcggcctccg cgagcggctc gaggtcgacc atcccggggt
     2761 tgcagcgtcg cgcgaagtcg cccgcgcggt ccagcacgta ggcgacgccg cccgacatgc
     2821 cggcggcgaa gttccgtccg gtgcggccga gcacgacgac gcggccgccg gtcatgtact
     2881 cgcaggcgtg gtcgcccacg ccctcgacga cggtgagcgc gccgctgttg cggacggcga
     2941 agcgctcgcc ggcgac
//
LOCUS       DATIAZ010000339        13323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_496663, whole genome shotgun sequence.
ACCESSION   DATIAZ010000339 DATIAZ010000000
VERSION     DATIAZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 13323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13323
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496663"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VLF19_11595"
     CDS             <1..293
                     /locus_tag="VLF19_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93941.1"
                     /translation="IPREQLEAIVPMETTYEEVLRRCGPEVEEREDLAAPRRKVLVYR
                     GRRDVPHRRWAWGWLAAVSFWDVERHEVEITLQDGVVQDSQVRVGRTRHAQP"
     gene            complement(290..652)
                     /locus_tag="VLF19_11600"
     CDS             complement(290..652)
                     /locus_tag="VLF19_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HSE93942.1"
                     /translation="MASRKMTARGRKRKGKKRGAGDSARGRRWVRHVMETSNAMDLPP
                     GIFTRAPKDIARGLKQSVLRSPRTKGTKFRSAMSMLNLYINRTGRNLGARDRRRLEAA
                     KGELRRVFGREPRPSGRR"
     gene            701..1039
                     /locus_tag="VLF19_11605"
     CDS             701..1039
                     /locus_tag="VLF19_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93943.1"
                     /translation="MAADVPAHPFARDPDCLLCRADKITPWFHEDDVCWIAECELCAV
                     PMVVWRWHGTRPPEEHVAQMRARLAEVATTELGEFYVDGHMRNIPDHWHCHARPRDGF
                     FGHGRQARRD"
     gene            complement(1108..1458)
                     /locus_tag="VLF19_11610"
     CDS             complement(1108..1458)
                     /locus_tag="VLF19_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93944.1"
                     /translation="MKIHNLYTDKTGESHFRDLEIELGQPTPSGRVSARLPATGIIFR
                     EVQPTYDLDWHPAPRRQYIINLDAGVRITASDGESRVIGAGEVILVEDTTGKGHLSKA
                     VENKVRHCIFVPVD"
     gene            1492..2328
                     /locus_tag="VLF19_11615"
     CDS             1492..2328
                     /locus_tag="VLF19_11615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSE93945.1"
                     /translation="MASAAGLSIVIVTWNARDAVARCLDALAAAPPAEPHDITVVDNA
                     STDGVPAMVRGRFPSVRLIETGTNLGFAAAANRGARVSSRDLVLLLNPDTIVPAGAVD
                     ALIGRLRERPDVAAIGPRIVDADGHPEWSWGDDLTPLAEARRRLLEAAVERDVPGARV
                     RLARLTGSEREVDWLTGACLLVRRDDAERTGFFDEGYFLYCEDADFCRALRARGRRVL
                     FSPVATVTHHRGRSAAAAGGRAREAWRASRLRYYARYAPRWLPLLRLWQRARGRPSAA
                     VR"
     gene            complement(2359..3474)
                     /locus_tag="VLF19_11620"
     CDS             complement(2359..3474)
                     /locus_tag="VLF19_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93946.1"
                     /translation="MGTTPKILVVDDNAANVKILQTRLSAEGYDVVTAADGEQALAAA
                     ATHRPDLILLDVMMPKLDGVEVCRRLKADTRFPFTPIVLVTAMSDARDVVAGLDAGAD
                     EYLTKPVDHQALLARVRSMLRIKTLHDTVQAQTEELARWNAILEQRVAEQVEELERVG
                     RLKRFFSPQLADLIVGGGTEDPLKTHRRQITVVFLDLRGFTAFAETAEPEEVMNVLRE
                     YHAEIGRLIMAHEGTLERFTGDGMMVFFNDPVPVPDPVHRAARMAVAMRDRVEELQVR
                     WQKLGYELRLGIGMADGYATLGAIGFEGRWDYAAVGTVTNLAFRLCSEAKGGQILVSS
                     KVAAALEATAELEDVGALTLKGFAKPVPALGLLRLRS"
     gene            3564..5204
                     /locus_tag="VLF19_11625"
     CDS             3564..5204
                     /locus_tag="VLF19_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSE93947.1"
                     /translation="MRRHSSAPREATARERQGRAVTFLLTRSPLGWLVDRVAAIRASV
                     HAKLLVAFLLITFLFIAMAGVSLRTIVATTHQSRLLHEAHERVEWSQQIEHALARQMH
                     QTALALLSRDEATIASILRENNRFNNTLAKLQDAASPDERELIQQIRVRQEDAMTAVA
                     DIANAIRDGRLDLAQAALLGREDRVYREIETLVGRLVAAENARMTGLRANVEAANRRS
                     LILTACFAVASVALALLCGFVISWSFIVPVREAHRFLAQVTAGDLGATIEVPNRDELG
                     GLALRMNQMSRELGRLVDEQQQAAAELRRLNERLQQASRAKSEFLANMSHELRTPLNA
                     ILGFTEMLLDDLYGDVPSELKEPLRDIQTNGRHLLRLINDVLDLSKIEAGRMELALGE
                     YSPAEVIDAVRASLRSLAADKGLEFVARVPRHLPVALADGGRITQCLLNLAGNAIKFT
                     REGRVEIAVEVAGADLVYSVSDTGIGIPKDELDNVFAEFRQVDATITREFGGTGLGLS
                     ITKKFVEMHGGRIWVESELGRGSTFSFSIPVHADGGAR"
     gene            5201..5575
                     /locus_tag="VLF19_11630"
     CDS             5201..5575
                     /locus_tag="VLF19_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE93948.1"
                     /translation="MKPRTVLYVEDNEFNRKIVRQLLRRTTYRLLEAADGESGVAMAK
                     QESPDLVLMDVQLPKMSGLDATRALRADPTTAQIPVLVVTSFALSGDDQRAMQAGASA
                     YLAKPYSPRDLLALIRKFAPES"
     gene            5596..6033
                     /locus_tag="VLF19_11635"
     CDS             5596..6033
                     /locus_tag="VLF19_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HSE93949.1"
                     /translation="MRIAWTGRARWAAIALVVALASPAGASGGQEEQFTPEFRAVHTS
                     YDPKHTPKIAAPESVKRGQWFDVVVSVGAGGEHPSLSEHFVRYIALYKDTAEIARIYL
                     HPVFSAPRVTFTIALDEGGMLRAMAEPTHSAAWETSRKIAVTP"
     gene            complement(6213..6899)
                     /locus_tag="VLF19_11640"
     CDS             complement(6213..6899)
                     /locus_tag="VLF19_11640"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSE93950.1"
                     /translation="MRVATLCVLAAACALLADTVGAGEIHGRALLAGAPPPPKKVPVT
                     IDQYVCGMDKDAEDLVVSPAREIKNVVVWLHNPPATAPSAATTAPVQMDQKSCVFTPR
                     VLLVPADGTVEFLNNDRLLHNLHSRSTSNMSFNRTQPRGRTIAIKFAKNPEFIRIDCD
                     LHSWMRGWVVVTDHPFYAVTDARGQFQFGSVPPGEYTLKTWHETLGETSRTITVGAGP
                     ATVTVELQPK"
     gene            7023..8015
                     /locus_tag="VLF19_11645"
     CDS             7023..8015
                     /locus_tag="VLF19_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785751.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HSE93951.1"
                     /translation="MRTVEIAPTFEAWQAAARALLREGVPPAEVAWREHDVSDAETGP
                     PAHVPPVARVPRQFLDLARQAASATDPGRWTALYEALWRIVHESHDLLERTRDPAVRH
                     LAALAAQGRREARRDEAAEVPALEAEGGAARFVPAGAGLDALREAAARCTGCDLYRHA
                     TQMVFGRGPAGARIVLVGEQPGDQEDVKGAPFVGPAGDVLDRALAEVGLDRERVYVTN
                     AVKHFKFVPRGKRRIHQTPRLGEIAACRPWLEAELDAVKPRVLTCLGATAARAIMGPD
                     FRLMQQRGRFFSTRWAPKTIATLHPSAVLRGQDDAAQARLYAMLRDDLRLVAAA"
     gene            8041..10365
                     /locus_tag="VLF19_11650"
     CDS             8041..10365
                     /locus_tag="VLF19_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSE93952.1"
                     /translation="MATIPSKLMGAEVKRKEDPRLVTGGSTYVGDLVLPGLHHVAFVR
                     SPHAHARIRGIDTSAALRRPGVRAVFTGSDIRGHCAPLPIAGAGEGGPSQDVGGRKHL
                     ALSLDRVRHAGEAVAAVIATSETAARDAVDDVRVDWEPLPAVTGVFAAMASGAPLLHE
                     DSARNIEHENRIAAGDADAAFKSAKRVVKQRMVNQRLCGVPMEGRAVLAAPDSATGGL
                     TVWVGNQAPHGFRNELAGALGLPQNLLRAIAPEVGGGFGVKFGVYPEDATLAVIARRL
                     RIPVRWVETRTEHMLATTHGRDQVTDIEAAVEDDGTIVALRMSVVANVGAYPIFTFIP
                     DLTLFMGVGVYKIANVDLRSTCVFTNTTPVAAYRGAGRPEAAYYVERLMDLIAAELGK
                     TPEEVRRRNFIPPDAFPYQAPTGQRYDSGEYDRALTKVLDLARLADLRKEQRQRLETR
                     DRHLLGIGTACYVEMCGFGPFESAVVRVEPGGAVTAYTGTSAHGQGHETTFAQIIADH
                     LGADFDKVVVRHGDTLNTPMGNGTGGSRSLAVGGTAILNATLKVQDKARQIAASMLEA
                     AAPDVVLTDGRYQVRGSPQSSVTLAQIAEQAYSAGLSESVESGLEATDFFRPPQLVYP
                     FGAHVAVVEVDRETGRVRLRDYVSVDDCGVRISPMIVAGQVHGGLAQGIAQALLEGLV
                     FGDDGQLLTGSLMDYAVPHADDLPPFTLAETVTPTPFNALGAKGIGEAATIGSTPAVV
                     NAVVDALRSFGVKHLDMPLRPERVWRALQASPRT"
     gene            complement(10414..12798)
                     /locus_tag="VLF19_11655"
     CDS             complement(10414..12798)
                     /locus_tag="VLF19_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSE93953.1"
                     /translation="MAHDQTKFDVEALLQAGEFPFRCEFSLRELARYWEREAEAGHPI
                     GRLIRERLAGAPEVVGPITDRAALDRHRDLVDLMMSAVFPPALREETLGAALVPFQLR
                     SLYATPTMERRGLDESGRLRGRSNMDPRTGLAYRILQAYALVLPKFYGIPLDVEYPLV
                     VTVDDRATGWERYLKTRFDPRFLAVEPIGDVPPLDDEARRRVLASVSDPVALMTLLPP
                     DRFAFRGFTVFTAVDVTDQEILSAIKRELIERESIVSSAPFKRLEERLRAYLRRPGLE
                     LGLAAMEGEQIFLLGHGEGLQHGCIFADSKHYQRRQFEGSIFEQAVKRGEPLVVDDLQ
                     TWPGRTAIEDALLGHGVRSIVVAPLHYQDEVIGVLELTSDRPGELNPSQAFRLREVLP
                     LFSMAVKRSMDELDTRIQAVIKEKCTAIHPVVEWRFRRAVMNTLEKSPGADGEIDLGP
                     IVFEGVHPLYALTDIRGSSLERARGIQADLLEQLRLAREVVRGGHATRALPALDELGY
                     RIDKHAAKIESGLSAGDELGVIAFLRTSVEPLFDHLAGFGPAVRERVEAYRAAVDPAL
                     GFVYRQRRRFEESITRLNDGISAYLDLEQQVAQSMYPHYFEKQKTDGVDHQIYIGASL
                     VEDGRFDPIYLRNLRLWQLMVTCGIAARAERLKTCCTMPLDTTHLILVQHAPLAIRFR
                     FDEKRFDVDGAYNARYEIVKKRIDKASVRNGAERLTQPGRIAVVYGHPAEAAEYHDYV
                     EYLQSLGYLTGGVEDLDLDELQGVRGLRALRVGIDLANPRLERPVTVADLTARG"
BASE COUNT         1915 a         4808 c         4706 g         1894 t
ORIGIN      
        1 tcatcccgcg cgagcagctg gaggcgatcg tgcccatgga gacgacgtac gaggaggtgc
       61 tgcgccggtg cgggccggag gtcgaggagc gggaagatct cgccgcgccg cgccgcaagg
      121 tcctggtcta ccggggacgg cgcgacgtcc cgcaccgacg gtgggcgtgg ggatggctgg
      181 cggcggtcag cttctgggac gtcgagcgtc acgaggtcga gatcacgctc caggacggtg
      241 tcgtgcagga cagccaggtg cgcgtcggcc ggacgcgcca cgcccagccc tagcgccggc
      301 cgctcggccg cggctctcgc ccgaagactc ggcgcagctc gcccttcgcg gcctccaggc
      361 gcctccgatc gcgcgcgccc aggttgcgcc ccgtccggtt gatgtagagg ttcagcatcg
      421 acatcgcgga ccggaacttc gtgcccttcg tgcgcgggct gcgcagcacc gactgcttga
      481 gcccgcgcgc gatgtccttc ggggcccgcg tgaagatccc gggtggcagg tccatggcgt
      541 tcgacgtctc catgacgtgc cggacccatc gccgcccgcg cgcggagtcg cccgccccgc
      601 gcttcttgcc cttgcgcttc ctgcccctgg cggtcatctt ccgggacgcc atgcattcag
      661 gataaccgct cgaaccgggg aagcgctgta cggtaggggc gtggccgccg acgtccccgc
      721 ccaccccttc gcccgtgacc cggactgcct gctgtgccgc gccgacaaga tcacgccgtg
      781 gttccacgag gacgacgtct gctggatcgc cgagtgcgag ctgtgtgccg tgccgatggt
      841 ggtgtggcgc tggcacggca cacgaccgcc cgaggaacac gtggcgcaga tgcgcgcgcg
      901 cctcgccgag gtcgcgacga cggagctcgg cgaattctac gtggacgggc acatgcgcaa
      961 catccccgac cactggcact gccacgcccg ccccagggat ggcttcttcg gccacggacg
     1021 ccaggcccgg cgtgactaga ccgggagcgt cgggttggtt agaggagggt cacggcttcg
     1081 ccggggcgct ctggggccct catgcggtca atccacgggc acgaagatgc agtgacgcac
     1141 cttgttctcc acggccttgc tcaggtgccc cttgcccgtc gtgtcctcga ccaggatcac
     1201 ctcgcccgcc ccgatcacgc gcgactcgcc gtcgctcgcg gtgatccgca cgccggcgtc
     1261 gaggttgatg atgtactgcc ggcgcggcgc ggggtgccag tcgaggtcgt aggtcggctg
     1321 cacctcgcgg aagatgatgc ccgtcgccgg cagccgcgcc gacacccggc cgctcggcgt
     1381 cggctggccg agctcgatct ccagatccct gaagtgcgac tctccggtct tgtccgtgta
     1441 gagattgtgg atcttcatgg cagcctcccg gccctatgct agcctccccg cgtggcgtca
     1501 gccgcgggac tctccatcgt catcgtcacg tggaacgcgc gtgacgccgt cgcccggtgt
     1561 ctcgacgcgc tggcggcggc gcctcccgcg gagccgcacg atatcaccgt cgtggacaac
     1621 gcttccaccg acggcgtgcc ggccatggtc cgcgggcgct tcccgtcggt gcgcctgatc
     1681 gagacgggca cgaacctcgg cttcgccgcg gccgccaacc gcggggcccg cgtctcgagt
     1741 cgcgacctcg tcctgctgct gaaccccgac acgatcgtcc ccgccggcgc ggtcgacgcg
     1801 ctgatcggcc gcctgcggga gcgtcccgac gtggcggcga tcggcccccg catcgtggac
     1861 gccgacggcc acccggagtg gtcgtggggc gacgacctga cgccgctcgc cgaggcacgg
     1921 cgccgcctgc tcgaggccgc cgtcgagcgt gatgtgcccg gcgcccgcgt ccggctcgcc
     1981 cgcctcaccg ggagcgagcg agaggtggac tggctcaccg gcgcgtgtct gctcgtccgc
     2041 cgcgacgatg ccgagcgcac cgggttcttc gacgaagggt atttcctcta ctgcgaggac
     2101 gcggacttct gccgcgccct gcgcgcccgc gggcggcgcg tgctcttctc gcccgtggcc
     2161 acggtcacgc accatcgcgg ccggtccgcg gcggcggcgg gcggacgtgc gcgcgaggcg
     2221 tggcgcgcga gccggctgcg ctactacgcc cgctacgcgc cgcgctggct gccgctgctg
     2281 cggctctggc agcgcgcacg cggacgccca tcggccgcgg tccgatagat cgcacggcct
     2341 cgcggaggcg cgaggccgtc agctccggag ccgcagcagg ccgagcgcgg gcaccggctt
     2401 ggcgaagccc ttgagggtca gggccccgac gtcctccagc tcggcggtgg cctcgagcgc
     2461 cgccgccacc ttcgacgaca cgaggatctg cccgcccttg gcctcggagc agagacgaaa
     2521 cgcgaggttc gtgaccgtgc ccacggcggc gtagtcccag cggccctcga acccgatcgc
     2581 cccgagggtg gcgtacccgt cggccatccc gatgccgagc cgcagctcgt agccgagctt
     2641 ctgccagcgc acctgcagct cctccacgcg gtcgcgcatc gcgaccgcca tccgggccgc
     2701 gcggtggacc gggtcgggca cgggaacggg atcgttgaag aagaccatca tgccgtcgcc
     2761 ggtgaagcgc tcgagggtgc cctcgtgcgc catgatgagg cgcccgatct cggcatggta
     2821 ctcgcgcaga acgttcatca cctcttcggg ctctgcggtc tcggcgaacg cggtgaagcc
     2881 ccggaggtcg aggaacacca ccgtgatctg ccggcggtgg gtcttcagcg ggtcctcggt
     2941 gccgccgccg acgatgaggt cggcgagctg cggcgagaag aagcgcttga ggcggcccac
     3001 ccgctcgagc tcctcgacct gctcggccac gcgctgctcg aggatcgcgt tccaccgggc
     3061 gagctcctcg gtctgggcct ggacggtgtc gtgcagggtc ttgatccgga gcatcgagcg
     3121 gacccgcgcc agcagcgcct ggtggtcgac gggcttggtg aggtactcgt cggcaccggc
     3181 gtcgaggccg gcgaccacgt ccctcgcgtc cgacatcgcg gtgaccagca cgatcggcgt
     3241 gaacggaaag cgcgtgtcgg ccttcaggcg gcggcacacc tcgacgccgt ccagcttggg
     3301 catcatgacg tcgagcagga tcaggtcggg ccggtgcgtc gccgcggccg ccagcgcctg
     3361 ctcgccgtcg gcggcggtca cgacgtcgta gccctcggcg ctgagccgcg tctggaggat
     3421 cttgacgttg gcggcgttgt cgtcgacgac gaggatcttg ggagtcgtgc ccacggccgg
     3481 tcgcccttgc gtctgcacta taccgttggc gatcggcagc gacaaggcgt gctatcctgc
     3541 ccgagaggcg gcacgtgcgc ggcatgagac gccattcgtc agcgccacgc gaggcgacgg
     3601 cgcgggagcg gcagggtcgg gccgtcacct ttctgctgac ccgcagcccg ctcggctggc
     3661 tcgtggaccg ggtcgccgcc atccgcgcct cggtccacgc gaagctcctc gtcgcattcc
     3721 tcctcatcac ctttctcttc atcgccatgg ccggcgtgag cctgcgcacg atcgtggcca
     3781 ccacccatca gagccggctc ctgcacgagg cgcacgagcg cgtggagtgg tcgcagcaga
     3841 tcgagcacgc gctggcccgc cagatgcacc agaccgcgct cgccctgctc tctcgcgacg
     3901 aggcgacgat cgccagcatc ctgcgtgaga acaaccgctt caacaacacg ctcgccaagc
     3961 ttcaggacgc ggcctcgccc gacgagcgcg agctgatcca gcagatccgc gtccgccagg
     4021 aagacgccat gacggcggtg gcggacatcg cgaacgccat ccgcgacggc cggctcgacc
     4081 tcgcgcaggc agcgctgctc gggcgcgagg accgggtcta ccgggagatc gagaccctcg
     4141 tcggccgtct ggtcgcggcg gagaacgcgc ggatgaccgg cctgcgggcg aacgtcgagg
     4201 ccgccaaccg ccggtcgctg atcctcaccg cgtgcttcgc ggtcgcctcg gtggccctgg
     4261 cgctgctgtg cggcttcgtg atctcgtggt cgttcatcgt cccggtccgc gaagcgcacc
     4321 ggttcctggc ccaggtcacg gccggcgacc tcggcgccac gatcgaggtg ccgaaccgcg
     4381 acgagctcgg cgggctcgcg ttgcggatga accagatgag ccgcgagctc ggccgcctcg
     4441 tcgacgagca gcagcaggcc gccgccgagc tgcgccgcct gaacgagcgg ctacagcagg
     4501 ccagccgcgc gaagtccgag ttcctcgcca acatgagcca cgagctgcgc accccgctga
     4561 acgccatcct cgggttcacc gagatgctgc tcgacgacct ctacggcgac gtcccgtccg
     4621 agctgaagga gccgctgcgc gacatccaga cgaacggccg gcacctgctc cggctcatca
     4681 acgacgtgct cgacctctcg aagatcgagg ccggccgcat ggagctggcc ctcggcgagt
     4741 actcgccggc cgaggtcatc gacgcggtgc gggcctcgct ccgctcgctc gccgccgaca
     4801 aggggctcga gttcgtggcc cgggtgccgc gccacctgcc cgtggccctc gccgacggcg
     4861 ggcgcatcac gcagtgcctc ctgaacctgg ccggtaacgc catcaagttc acccgcgagg
     4921 ggcgcgtgga gatcgccgtc gaggtggccg gcgccgacct cgtctacagc gtgtccgaca
     4981 ccggcatcgg catccccaag gacgagctcg acaacgtgtt cgcggagttc cggcaggtgg
     5041 acgcgacgat cacccgggag ttcggcggca ccgggctcgg gctcagcatc acgaagaagt
     5101 tcgtcgagat gcacggcggc cggatctggg tcgagagcga gctgggccgg ggctcgacct
     5161 tctcgttctc gatccccgtg cacgcggacg gaggcgcgcg atgaagccgc ggacggtgct
     5221 gtacgtggag gacaacgagt tcaaccggaa gatcgtgcgc cagctcctcc gccgcacgac
     5281 gtaccgtctg ctcgaggccg ccgacggcga gtccggggtg gccatggcga agcaggagag
     5341 cccggacctc gtgctcatgg acgtgcagct gccgaagatg tccggtctcg acgccacgcg
     5401 cgcgctccgc gccgatccga cgaccgcgca gatcccggtg ctggtggtca cgtccttcgc
     5461 gctcagcggt gacgatcagc gggccatgca ggccggcgcc tccgcgtacc tcgccaagcc
     5521 ttacagcccc cgggacctgc tcgcgctcat ccgcaagttc gcgccggaaa gctgaccact
     5581 tcgaaggagg acagcatgcg aatcgcctgg accggccgcg cccgctgggc ggccatcgcg
     5641 ctcgttgtcg cgctggcgtc gccggccggg gcctccggcg ggcaggaaga gcagttcacg
     5701 ccggagttcc gtgcggtgca cacgagctac gaccccaaac acaccccgaa gatcgccgcg
     5761 cccgaatcgg tcaagcgcgg gcagtggttc gacgtggtgg tgtcggtcgg ggccggaggg
     5821 gagcacccgt ccctctcgga gcacttcgtg cgctacatcg cgctctacaa ggacacggcg
     5881 gagatcgccc gcatctacct gcaccccgtg ttctcggcac cgcgggtgac gttcaccatc
     5941 gcgctcgacg agggcgggat gctgcgggcc atggccgagc cgacgcactc cgcggcctgg
     6001 gagacctccc ggaagatcgc cgtcacgccg tgactcctcg gagaggcttc gcccatcatc
     6061 gcgacgggcg gggccgagct tacggcgcgc tctcgccctg cgtccctccc agaacgggtt
     6121 gtgcgggcgc ggcccgcgct cgagcaggcg acacccgtcg gttgggcgac cgctcggagc
     6181 gccgctccgt cgctggagaa tcccggagag atctacttcg gctgcagctc tacggtgacg
     6241 gtggcggggc cggcgcccac ggtgatcgtg cggctggtct cgccgagcgt ctcgtgccag
     6301 gtcttgaggg tgtactcgcc gggcgggacg ctgccgaact ggaactgtcc gcgagcgtcg
     6361 gtgaccgcgt agaaggggtg gtccgtcacc accacccacc cccgcatcca cgagtgcagg
     6421 tcgcagtcga tgcggatgaa ctcggggttt ttcgcgaact tgatcgcgat cgtccgcccc
     6481 cgcggctgcg tgcggttgaa cgacatgttg ctcgtcgagc ggctgtggag gttgtgcagg
     6541 aggcggtcat tgttcaggaa ctccacggtg ccgtcggccg gcacgagcag gacgcgcggc
     6601 gtgaacacgc acgatttctg atccatctgg acgggcgcgg tcgtcgccgc tgacggcgcc
     6661 gtcgccggcg ggttgtggag ccacacgacg acgttcttga tctcccgcgc cggcgagacc
     6721 accagatcct cggcgtcctt gtccatcccg cagacgtact ggtcgatggt gacgggcacc
     6781 ttcttcggcg gcggcggcgc accggcgagc aaggcgcggc cgtggatctc cccggctccc
     6841 acggtgtcgg cgaggagcgc gcaggcggcg gcgagaacgc acagcgtcgc gacgcgcatc
     6901 gctcgacacc ataccaccta atctcgcgaa tgcagaggcc gcggccacgt ggtgcgctcc
     6961 ggagggcccg tgcatgcccg gagccccgcc cggccgcttc ccccgcgcgg tacgatgtgg
     7021 ccgtgcgcac cgtggagatc gcgcccacgt tcgaggcctg gcaggcggcg gcccgggccc
     7081 tgctccgcga gggcgtgcca cccgcggaag tcgcgtggcg ggagcacgac gtgtccgacg
     7141 ccgagactgg accaccggcg cacgtgccac cggtcgcgcg agtgccccgg cagttcctcg
     7201 acctcgcccg ccaggcggcg agcgcgacgg acccgggccg ctggacggcg ctctacgagg
     7261 cgctctggcg catcgtccac gagagccacg acctcctgga aaggacgcgc gatcccgccg
     7321 tgcgccacct cgccgcgctc gcggcgcagg gacggcgtga agcccggcgt gacgaggccg
     7381 ccgaggtgcc ggcgctcgag gccgaaggcg gcgcggcgcg cttcgtcccc gccggcgccg
     7441 gcctggacgc gcttcgcgag gccgccgcgc gctgcacggg ctgcgacctg tatcgccacg
     7501 cgacgcagat ggtcttcggc cgcggcccgg ccggcgcgcg catcgtgctc gtcggagagc
     7561 agcccggcga ccaggaggac gtcaagggcg cgccgttcgt ggggccggcc ggggatgtgc
     7621 tcgatcgcgc gctcgccgag gtcgggctcg atcgcgagcg cgtgtacgtg accaacgcgg
     7681 tcaagcactt caagttcgtc ccgcggggca agcggcggat ccaccagacg ccgcgcctcg
     7741 gggagattgc cgcctgccgg ccctggctcg aggccgagct ggacgccgtg aagccccggg
     7801 tgctcacgtg cctcggcgcg accgcagcgc gggccatcat ggggccggac ttccggctca
     7861 tgcagcagcg cgggcgcttc ttctccacgc gctgggcgcc gaagaccatc gccacgctcc
     7921 atccgtcggc ggtgctgcgc ggccaggacg acgccgcgca agcgcggctc tacgccatgc
     7981 tgcgcgacga cctgcggctg gtggcggcgg cgtagggaca atccgctccg gaggatccac
     8041 atggcgacca ttccctcgaa gctgatgggt gcggaggtca agcgcaagga agacccgcgc
     8101 ctggttacgg gcggctccac ctacgtgggc gacctcgtgc tgcccgggct gcaccacgtg
     8161 gcgttcgtgc gcagccctca cgcccacgcg cgcatccgcg gcatcgacac gtcggcggcg
     8221 ctgcgccgtc ccggcgtgcg cgcggtcttc accggcagcg acatccgcgg ccactgcgcg
     8281 ccgctgccca tcgccggcgc cggtgagggc ggccccagcc aggacgtggg cggccgcaag
     8341 cacctggcgc tgtcgctcga ccgcgtccgc cacgccggcg aggccgtggc cgccgtcatc
     8401 gcgaccagcg agacggccgc gcgcgacgcc gtggacgacg tgcgcgtgga ctgggagccg
     8461 ctgcccgcgg tcaccggcgt cttcgcggcg atggcgagcg gcgcgccgct cctccacgag
     8521 gactccgcgc ggaacatcga gcacgagaac cgcatcgccg ccggcgacgc cgacgccgcc
     8581 ttcaagagcg ccaagcgcgt ggtcaagcag cggatggtca accagcgcct ctgcggcgta
     8641 ccgatggagg gccgggccgt gctcgcggcg cccgactccg ccacgggcgg gctcaccgtc
     8701 tgggtcggaa accaggcgcc ccacgggttc cgcaacgagc tggccggcgc gctcggcttg
     8761 ccgcagaacc tcctgcgcgc gatcgccccc gaggtggggg gcggcttcgg cgtgaagttc
     8821 ggcgtctacc cggaggacgc gacgctcgcg gtcatcgccc ggcggctgcg catcccggtg
     8881 cgctgggtgg agacccgcac cgagcacatg ctcgccacga cccacgggcg cgatcaggtg
     8941 accgacatcg aggccgcggt ggaggacgac ggcaccatcg tcgcgctccg catgagtgtc
     9001 gtcgccaacg tcggcgccta cccgatcttc acgttcatcc ccgacctcac gctcttcatg
     9061 ggcgtcggcg tctacaagat cgccaatgtg gaccttcgct cgacctgcgt cttcaccaac
     9121 accacgccgg tcgcggccta ccggggcgcc gggcgcccgg aggcggcgta ctacgtcgag
     9181 cgactcatgg acctgatcgc cgccgagctc ggcaagacgc cggaggaggt gcggcggcgc
     9241 aatttcatcc cgcccgacgc cttcccctac caggcgccca cgggccagcg ctacgacagc
     9301 ggcgagtacg accgcgcgct gacgaaggtc ctggacctgg cccgcctggc cgacctgcgc
     9361 aaggagcagc gccagcggct cgagaccagg gatcgtcacc tgctgggcat cggcaccgcc
     9421 tgctacgtcg agatgtgcgg cttcggcccg ttcgagagcg cggtggtccg cgtggagccc
     9481 gggggcgcgg tcacggccta caccggcacg tcggcgcacg ggcagggcca cgagacgacg
     9541 ttcgcgcaga tcatcgccga ccacctcgga gcggacttcg acaaggtcgt cgtgcgccac
     9601 ggggacacgc tcaacacgcc gatggggaac ggcaccggcg gcagccgcag cctggccgtc
     9661 ggtggcaccg ccatcctcaa cgcgaccctc aaggtgcagg acaaggcgcg gcagatcgcc
     9721 gcgtcgatgc tggaggccgc cgcgccggac gtcgtcctca ccgacggacg ctaccaggtg
     9781 cgcggctcgc cgcagagcag cgtcacgctc gcccagatcg ccgagcaggc ctactcggcg
     9841 ggactatccg aaagcgtgga gtcggggctc gaggccacgg acttcttccg cccgccgcag
     9901 ctcgtctacc ccttcggcgc ccacgtggcc gtcgtcgagg tggaccgcga gacgggccgg
     9961 gtgcgcctgc gcgactacgt gtcggtggac gactgcggcg tgcggatcag cccgatgatc
    10021 gtcgccggcc aggtgcacgg cgggctcgcc cagggcatcg cccaggcgct gctggaaggg
    10081 ctcgtcttcg gcgacgacgg ccagctcctg accggctcgc tcatggacta cgcggtgccc
    10141 cacgccgacg acctgccgcc gttcacgctg gcggagaccg tgacgcccac gccgttcaac
    10201 gcgctcggtg ccaagggcat cggcgaggcg gccacgatcg gctcgacgcc ggccgtggtc
    10261 aacgcggtgg tcgacgccct ccgctcattc ggcgtcaagc acctggacat gccgctccgc
    10321 cccgagcgcg tctggcgtgc gctgcaggcc agcccccgga cctgagcggc tcgcgtcccg
    10381 gatcggcggt tcgcgccctt cagatgcggg acgctaaccg cgcgcggtga ggtcggcgac
    10441 agtgaccggg cgctcgaggc gcgggttggc gaggtcgatg cccacgcgca gcgcgcggag
    10501 accccgcacg ccctgcagct cgtccaggtc gaggtcctcg acgccgcccg tcaggtagcc
    10561 gagcgactgc aggtactcga cgtagtcgtg gtactcggcg gcctcggccg ggtggccgta
    10621 gacgaccgcg atgcggccgg gctgcgtgag ccgctcggcg ccgttccgta cgctggcctt
    10681 gtcgatgcgc ttcttcacga tctcgtaccg ggcgttgtag gcgccatcga cgtcgaagcg
    10741 cttctcgtcg aagcgaaagc ggatggccag cggcgcgtgc tggaccagaa tcaggtgcgt
    10801 cgtgtcgagc ggcatggtgc agcacgtctt gagccgctcg gcgcgcgcgg cgatgccgca
    10861 cgtcaccatg agctgccaga ggcgcaggtt gcgcaggtag atgggatcga agcggccatc
    10921 ctcgacgagc gaggcgccga tgtagatctg gtggtcgacg ccgtcggtct tctgcttctc
    10981 gaagtaatgc gggtacatcg actgcgccac ttgctgctcc aggtcgagat aggcggagat
    11041 accgtcgttc agccgcgtga tgctctcctc gaacctgcgc cgctgacggt agacgaagcc
    11101 gagcgccgga tcgacggcgg cgcggtaggc ctccacgcgc tcgcgcacgg ctggcccgaa
    11161 gcccgccagg tggtcgaaga gcggctccac gctcgtgcgg agaaacgcga tcacgcccag
    11221 ctcgtccccc gccgagaggc cgctctcgat cttcgccgcg tgcttgtcga tgcggtaacc
    11281 cagctcgtcg agcgcgggca gcgcccgggt ggcgtgcccc ccccgcacga cctcgcgggc
    11341 gaggcgcagc tgttcgagca ggtccgcctg gatcccgcgc gcccgctcga gggacgagcc
    11401 gcggatgtcg gtgagcgcgt agagcggatg gacgccctcg aagacgatcg gccccaggtc
    11461 gatctcgccg tcggcgccgg ggctcttctc gagggtattc atcaccgcgc gccggaagcg
    11521 ccactccacg accggatgga tggccgtgca cttctccttg atgacggcct ggatgcgcgt
    11581 gtcgagctcg tccatgctgc gcttgacggc catcgagaac agcggaagca cctcgcgcaa
    11641 ccgaaaggcc tgtgagggat tcagctcgcc cggccggtcg gaggtcagct cgagcacgcc
    11701 gatgacctcg tcctggtagt gcagcggcgc cacgacgatg ctccggaccc cgtgcccgag
    11761 caaggcgtct tcgatcgctg tccgccccgg ccaggtctgg aggtcgtcga ccacgagcgg
    11821 ctctccgcgc ttgacggcct gctcgaagat cgaaccctcg aactgacggc gctggtagtg
    11881 cttcgagtcg gcgaagatgc agccgtgctg cagcccctcg ccgtggccga ggaggaagat
    11941 ctgctcgccc tccatggccg ccagccccag ctccaggccg ggccgccgca ggtaggcgcg
    12001 caggcgctcc tccagccgct tgaagggcgc cgaggagacg atcgactcgc gctcgatcag
    12061 ctcgcgcttg atggcggaga ggatctcctg gtccgtgacg tccaccgccg tgaaaacggt
    12121 gaagccccgg aacgcgaagc ggtcgggggg cagcagggtc atcagcgcca ccgggtccga
    12181 gacgctggcg agcacgcgcc ggcgcgcctc gtcgtcgagc ggcggcacat cgccgatcgg
    12241 ctccaccgcg aggaagcgcg gatcgaagcg cgtcttgagg tagcgctccc agcccgtcgc
    12301 gcggtcgtcc acggtcacca cgagggggta ctcgacgtcc agcgggatcc cgtagaactt
    12361 cgggagcacg agggcgtagg cctgcaagat gcgataggcg agccccgtgc gcggatccat
    12421 gttgctgcgg ccgcgcagcc gtccgctctc gtcgagcccg cggcgctcca tggtcggcgt
    12481 ggcgtagagg ctgcgcaact ggaacgggac cagcgcagcc cccagcgtct cttcccggag
    12541 cgccggcggg aagacggccg acatcatcag atcgacgaga tcgcggtggc ggtcgagcgc
    12601 cgcgcggtcc gtgatcggcc cgaccacctc gggtgctccg gccagccgct cgcggatcag
    12661 acgcccgatg ggatgccccg cctcggcctc gcgctcccag taacgggcca gctctcgcag
    12721 gctgaactcg caccgaaacg ggaactcgcc ggcctggagg agcgcctcca cgtcgaactt
    12781 cgtctgatcg tgcgccattc cgcctcctgc gcgtcgcgcg acgacaccgg tcgccgcgtc
    12841 gtcattgtaa caaccgcctg gcgggcccgg aacggcaagg ccccttccca cgatcgaaac
    12901 ccctcccgtg gtccggcgag acggatagag cagcggggcc ccgtccgctg cgtccatacc
    12961 gtagtagatg cgcgaaaccc ccgcctggta gcaggcgatc tcgtgcatcg cacaaggccg
    13021 cgaactcacg tagttctcgc cacccggagg ctcgggaaga catgacgacc ggaccgacgg
    13081 cggagccggt tcagctggcc aacacggccg caagcgccga acgcctcgac aaacagctcc
    13141 cgcagcggtc ggggcgaaga tcagcgcggt gtcggggcga ccctcgcgcc ggagcgcgtc
    13201 cacacccggc ggggcgggat cacgggtgac gtgctccgcc aggtcaagac tggaatgtcc
    13261 gcacgatcag cgcggcgccg acgagaaaca gtgccgcgcc gatgatgcga gccgccgtgg
    13321 tgg
//
LOCUS       DATIAZ010000340         6021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1812066, whole genome shotgun sequence.
ACCESSION   DATIAZ010000340 DATIAZ010000000
VERSION     DATIAZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812066"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VLF19_11660"
     CDS             complement(<1..1435)
                     /locus_tag="VLF19_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSE93954.1"
                     /translation="MRLESITLHGFKSFSERTEVEVLPGITCIVGPNGCGKSNISDAV
                     RWALGEQSAKSLRGQKMEDVIFYGSASRKAVGLAEVELKFSNEDAAVAVPWTEIAVAR
                     RLYRTGESEYLLNKTTTRLRDILDLFSGTGANPRAYSVMDQDKLNHVLTAKPHERRVF
                     IEEAAGIARYKQQRNETQGKLEAARQNLVRVRDVMDEVKRQLGSLERQARKAQQYKAL
                     ESERRDLRLALVAADHAALVAEGEQLEGELTSRRHREEELRVRQSHLGAREASQRELI
                     QASDHRLGDLRQSLQKVQAEMERLLERREQMAAQLREAVEEGVRLDDEIRVTTERLAA
                     IAGEREATRVTLAETERLVGERTEAALMLEDQVARHRAGLVDGRGRLEGLRLEQVRLA
                     GERTDLTRSAGELRERALQLSRRAERLAAELAGAEAEAGRLASARAALTTARDRAVAD
                     LDALSSDREQLAAALAGPQGELAEVEAQ"
     gene            complement(1561..4374)
                     /gene="secA"
                     /locus_tag="VLF19_11665"
     CDS             complement(1561..4374)
                     /gene="secA"
                     /locus_tag="VLF19_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSE93955.1"
                     /translation="MLTSLIRTVFGTKHERDVKRMMPAVQAINALESAMQALGDAALR
                     AKTDELRRRVADGAAANDLLVEAFAVCREAGRRTIGMRHFDVQLIGGMVLHGGTIAEM
                     ATGEGKTLVATLPAYLNALTGKGVHIVTVNDYLAKRDAQWMGPVYHALGLSVGVIQHE
                     ASFLYDPAYVTPDVRLTALRPCTRTEAYRADVTYGTNNEFGFDYLRDNMRFTREELVQ
                     RELHYAIVDEVDSILIDEARTPLIISGPADESTELYYKIDRVIPRLKRAATIVEGKLS
                     EIEQTAEGDYIVDEKSKAVSLTEQGIASCERLLNVDNLYDPRNIDVLHHIQQALRAHA
                     LYRRDVDYVVKDGQVIIVDEFTGRLMPGRRWSDGLHQAVEAKEAVRIERENQTLATIT
                     FQNYFRMYDKLAGMTGTAETEAEEFAKIYKLDVTVVPTNRALTRLNHPDVVYKTEREK
                     FNAVVEDIIRCHERGQPVLVGTISIEKSERLSLLLKKRGVKHEVLNAKYHEREAEIVA
                     QAGRAGAVTIATNMAGRGTDILLGGNPDFLSKELLRRKGHDPATATAEARAAALAEAR
                     KITEPEHQKVVSVGGLHIVGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSLEDDLLR
                     IFGSHRVQRIMDRLGMEEGEPIEHRLVTRAIATAQKRVENHNFEIRKHLLEYDDVMNK
                     QREIVYGMRRQILDGADQHETVGEWIGELVGGVVDAHAPESAHPEDWDLAALSETLHR
                     QFDVRIPSARFMDVASRDGLEELCGAAVAERYTARERELGAELLRALERHEMLIVIDQ
                     QWKDHLLSIDHLKEGIGLRGYGQRDPLTEYKREAFDLFQEMADRVKATVVERLFKVQV
                     VREGPVELPTITAWADARESRGELPAAPAGSPLTASASRPAVAASRPAPRTASGEKVG
                     RNDPCPCGSGKKYKKCCYLKQSA"
     gene            complement(4551..>6021)
                     /gene="recN"
                     /locus_tag="VLF19_11670"
     CDS             complement(4551..>6021)
                     /gene="recN"
                     /locus_tag="VLF19_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HSE93956.1"
                     /translation="AVFDVAGRREVLAVLADAGLAAEDGAVVVRRELTRSGRHRAFVN
                     DCAVTVGLLERLGDQLVDVHGQHEHQRLLEPARQLDLLDRFASAEAARERVGAAFAAW
                     RAAAEELAGLRAAARDRAQREDLLRFQVSELDAARLRVGEEDELRRERRRLQHAERLA
                     KGLAEASALLAEDPEAAAGRLARAARIFRDLGRLDPGFEAPAALLDGASAQVEEALLG
                     VRGLRASIEAEPGRLEALDDRIDALARLKRKYGDSEELMLAHREAAAAELERLVRHEE
                     TQAEAGRRVDGARHALDEAAAILGQRRREAATRLGPLVERELRALGMERARFEIGLHT
                     LPPGEMSARGAERAELVFSANPGEDLRPLARIASGGELSRTMLALQVVLARVEPVPTM
                     VFDEVDAGVGGGAAGVVADKLAAVAAGRQVLCVTHLAQVAARGQHHLRVAKSVRGGRT
                     RARVAVLGAAERIDEVARMLGGEPPSETARRHARELFRG"
BASE COUNT          771 a         2231 c         2068 g          951 t
ORIGIN      
        1 tctgggcctc gacctccgcg agctcgccct ggggcccggc cagagcggcc gcgagctgct
       61 cgcggtcgct cgagagcgcg tcgagatcgg cgacggcgcg gtcacgagcc gtcgtgagcg
      121 ccgcgcgcgc cgatgcgagc cggccggcct cggcctcggc gcccgccagc tcggcggcca
      181 gccgctcggc gcgtcgcgag agctgcaggg cgcgctcgcg cagctcaccc gccgagcgtg
      241 tgaggtccgt gcgctctccc gccagtctca cctgctcgag ccgcagcccc tcgagtcggc
      301 cccgcccgtc gaccaggcct gcgcggtggc gcgccacctg gtcctcgagc atcagcgcgg
      361 cctcggtgcg ctcgcccacg agccgctccg tctccgcgag cgtcacgcgc gtcgcctcgc
      421 gctcgcccgc gatggcggcc aggcgctcgg tggtcacgcg aatctcatcg tcgaggcgca
      481 cgccttcctc gacggcctcg cggagctggg ctgccatctg ctcgcgccgc tccagcagcc
      541 gctccatctc cgcctggacc ttctgcagcg actgccggag gtcgccgagg cggtggtcgc
      601 tggcctggat cagctcgcgc tgcgaggcct ctcgcgcgcc gaggtggctc tggcgcacgc
      661 gcagctcctc ctctcgatgc cggcggctgg tgagctcccc ctcgagctgc tcgccctcgg
      721 ccaccagcgc cgcgtggtcg gcggcgacca gcgccagccg caggtcccgc cgctcgctct
      781 cgagcgcctt gtactgctgg gccttccgcg cctgccgctc gagcgagccg agctggcgct
      841 tgacctcgtc catcacgtcc ctcacgcgga cgagattctg acgggccgcc tcgagcttgc
      901 cctgcgtctc gttccgctgc tgcttgtacc gcgcgatgcc cgcggcctcc tcgatgaaca
      961 cgcgccgctc gtgaggcttc gccgtgagca cgtgattcag cttgtcctgg tccatcaccg
     1021 agtacgcgcg cggattggcg ccggtgccgg agaagagatc gaggatgtcg cgcaggcggg
     1081 tggtcgtctt gttgagaagg tactcgctct cgcccgtgcg gtagagccgc cgggcgaccg
     1141 cgatctcggt ccacgggacg gcgacggccg cgtcctcgtt gctgaacttc aactcgacct
     1201 cggcgaggcc cacggccttg cgcgacgcgg aaccgtagaa gatgacgtct tccatcttct
     1261 gcccgcgcag cgacttcgcg ctctgctcgc cgagggccca gcgaaccgca tcggagatgt
     1321 tgctctttcc gcacccgttc gggcccacga tgcaggtgat cccgggcagg acctcgacct
     1381 cggtgcgctc ggagaacgac ttgaagccgt gcagggttat ggactcgagg cgcatgaatt
     1441 cttctagcac ggacccccgg cacaaaccaa gatctaaagc accagggata ggggaattcc
     1501 gcccgcggac ccccagatct ggcccctgtc cccgggggtc gcccggtcaa gccgctcggt
     1561 tcaggcgctc tgcttcagat agcagcactt cttgtacttc ttcccggacc cgcacgggca
     1621 cgggtcgttg cggccgacct tctcccctga cgccgtgcgg ggggcgggcc gcgaggccgc
     1681 caccgccggc cgcgaggccg aagccgtcaa cggcgagccg gcgggggcgg cgggcagttc
     1741 cccccgcgac tcgcgggcgt cggcccacgc ggtgatcgtc ggcagctcca ccggcccctc
     1801 gcgcacgacc tgcaccttga agagtcgctc gacgacggtc gccttcacgc gatcggccat
     1861 ctcctggaag agatcgaagg cctcccgctt gtactccgtg agcggatcgc gctggccgta
     1921 gccgcgcagc ccgatgcctt ccttgaggtg gtcgatcgac agcaggtggt ccttccactg
     1981 ctggtcgatg acgatgagca tctcgtggcg ctcgagcgcc ctcagcagct cggcgccgag
     2041 ctcacgctcc cgcgcggtgt accgctccgc cacggccgcg ccgcagagct cctcgagccc
     2101 atcccgggac gccacgtcca tgaagcgggc actggggatg cggacgtcga actgtcggtg
     2161 cagcgtctcg ctcagcgccg ccaggtccca gtcctccggg tgggcgctct ccggggcatg
     2221 cgcatcgacc acgccgccga cgagctcgcc gatccattcg ccgaccgtct cgtgctggtc
     2281 cgcgccatcc aggatctggc ggcgcatacc gtagacgatc tcgcgctgct tgttcatgac
     2341 gtcgtcgtac tcgagcaggt gcttgcggat ctcgaagttg tggttctcca cacgcttctg
     2401 ggcggtggcg atggcccggg tgacgagccg gtgctcgatg ggctcgcctt cctccatgcc
     2461 caggcggtcc atgatgcgct ggacgcgatg cgagccgaag atccgcagca ggtcgtcctc
     2521 gagcgacaag tagaagcggg aggagccagg gtcgccctgc cggccggagc ggccgcgcag
     2581 ctggttgtcg atgcgccgag actcgtggcg ctcggtgccg acgatgtgga ggccgccgac
     2641 cgacacgacc ttctggtgct ccggctcggt gatcttccgc gcctccgcga gcgcggccgc
     2701 gcgggcctcc gcggtcgccg tcgccgggtc gtgccccttc cgccgcagca gctccttcga
     2761 caggaagtcg ggattgccgc cgagcaggat gtccgtgccc cggcccgcca tgttggtggc
     2821 gatggtcacg gcaccggcgc ggccggcctg ggcgacgatc tccgcctccc gctcgtggta
     2881 cttggcgttg agcacctcgt gcttgacgcc gcgcttcttg agcagcagcg acagccgttc
     2941 ggacttctcg atggagatcg tacccaccag caccggctgg ccgcgctcgt gacacctgat
     3001 gatgtcctcg acgaccgcgt tgaacttctc ccgctcggtt ttgtagacca cgtccgggtg
     3061 gttcaagcgg gtcagcgccc ggttggtggg gaccaccgtg acgtcgagct tgtagatctt
     3121 cgcgaactcc tccgcctcgg tctcggcggt gccggtcatg ccggcgagct tgtcgtacat
     3181 ccggaaatag ttctggaagg tgatcgtcgc gagcgtctgg ttctcgcgct cgatgcgcac
     3241 ggcctccttg gcctccacgg cctggtgcag gccgtccgac caccgccggc cgggcatcag
     3301 gcggcccgtg aactcgtcga cgatgatgac ctgcccgtcc ttgaccacgt agtccacgtc
     3361 gcgccggtac agcgcgtgcg cccggagcgc ctgctggatg tgatgcagga catcgatgtt
     3421 gcgcggatcg tagaggttgt cgacgttgag caggcgctcg caggaagcga tgccctgttc
     3481 ggtgagcgac accgccttcg acttctcgtc gacgatgtag tcgccttcgg ccgtctgctc
     3541 gatctccgac agcttcccct cgacgatggt cgccgcccgc ttcagcctcg ggatgacgcg
     3601 gtcgatcttg tagtagagct cggtggactc gtcggcgggc ccggagatga tcagtggcgt
     3661 gcgggcctcg tcgatgagga tcgagtcgac ctcgtccacg atcgcgtagt gcagctcccg
     3721 ctggaccagc tcctcgcggg tgaaccgcat gttgtcgcgg aggtagtcga agccgaactc
     3781 gttgttggtc ccgtacgtga cgtccgcccg gtaggcctcg gtgcgggtgc acgggcgcaa
     3841 ggccgtcagc cggacgtcgg gcgtcacgta ggcaggatcg tagaggaacg acgcctcgtg
     3901 ctggatcacg ccgaccgaga gcccgagcgc gtggtacacc gggcccatcc actgcgcgtc
     3961 gcgcttggcg aggtagtcgt tgacggtgac gatgtgcacg cccttgcccg tcagcgcgtt
     4021 caggtaggcc ggcagggtcg ccaccagcgt cttgccctca ccggtcgcca tctcggcgat
     4081 ggtgccgccg tgcagcacca tgccgccgat gagctgcacg tcgaagtgtc gcatgccgat
     4141 cgtgcgccgc ccggcctccc ggcacaccgc gaaggcctcg acgagcagat cgttcgccgc
     4201 ggcgccgtcc gccacgcgcc ggcgcagctc gtccgtcttc gcgcgcagcg ccgcgtcgcc
     4261 gagagcctgc atggccgact cgagcgcatt gatcgcctgg acggccggca tcatgcgctt
     4321 gacgtcccgc tcgtgcttgg tgccgaagac cgtgcggatg aggctggtca gcatcgtctg
     4381 cggacattat atacaggccg gctccggggc ggccgcgttc atgtcgcaag tgcgtcggag
     4441 ggcgccgcag accgctacaa gtgtacgctt gctctcggct cgagcgccgg cttgccggcg
     4501 caatccggtt ccggaggagg cctcggaggg gcttcccccc tccgagcgac tcagccccgg
     4561 aacagctcgc gcgcgtggcg gcgcgcggtc tcgctcgggg gctcgccgcc gagcatgcgg
     4621 gccacctcgt cgatccgctc ggcggcgccg agcacggcga ccctcgcacg ggtgcggccg
     4681 ccccgcaccg acttggcgac gcgcaggtgg tgctgcccgc gtgccgcgac ctgagccagg
     4741 tgcgtgacgc agaggacctg acgcccggcg gccacggccg cgagcttgtc ggcgacgacg
     4801 ccggcggcgc cgccgcccac tccggcgtcc acctcgtcga agaccatggt cggcaccggc
     4861 tcgacgcggg ccagcaccac ctgcaaggcc agcatcgtcc gcgagagctc cccgcccgag
     4921 gcgatgcgcg ccagcggccg cagatcttcc ccggggttcg ccgagaagac cagctcagcg
     4981 cgctcggcgc cccgagccga catctcgccc ggtgggagcg tgtgcaggcc gatctcgaag
     5041 cgcgcccgct ccatgccgag ggcgcggagc tcgcgctcga ccagcggtcc gaggcgagtg
     5101 gcagcctcgc ggcgccgctg cccgagtatt gcggccgcct cgtccagcgc gtgccgcgcg
     5161 ccgtccacgc gtcgccccgc ctcggcctgc gtctcctcgt gacgcacgag gcgctcgagc
     5221 tcggctgccg ccgcctcgcg gtgggccagc atgagctcct cactgtctcc gtacttgcgc
     5281 ttgagacgcg ccagagcgtc gatccggtcg tcgagcgcct cgagccgtcc gggctcggcc
     5341 tcgatgctcg cgcgcaggcc gcggactccg agcagcgcct cctccacctg cgccgaggcg
     5401 ccgtcgagca gggcggccgg cgcctcgaac cccgggtcga gccggccgag gtcgcggaag
     5461 atgcgggcgg cccgcgccag gcgtccggcc gcggcctccg ggtcctcggc caggagcgcc
     5521 gaggcctcgg cgagaccctt ggcgagccgc tcggcgtgct gcaggcgtcg ccgctcccgg
     5581 cgcagctcgt cctcctcccc cactctcaga cgcgcggcgt cgagctccga gacctggaag
     5641 cgcagcagat cctcacgctg ggcccggtcg cgcgccgccg cgcggagtcc ggcgagctcc
     5701 tccgcggccg cgcgccacgc cgcgaaggcc gcgccgaccc gctcgcgcgc cgcctccgcg
     5761 gaggcgaagc ggtccagcag gtcgagctgg cgcgccggct ccagcagccg ctggtgctcg
     5821 tgctggccgt ggacgtccac gagctggtcg ccgaggcgct ccagcagacc gacggtgacc
     5881 gcgcagtcgt tgacgaaggc ccggtgacgt ccggagcgcg tcagctcgcg ccgcacgacc
     5941 accgcaccgt cctcggccgc caggccggca tccgccagca cggcgagcac ctcacgccgg
     6001 ccggccacgt cgaagacggc c
//
LOCUS       DATIAZ010000341         4981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1154351, whole genome shotgun sequence.
ACCESSION   DATIAZ010000341 DATIAZ010000000
VERSION     DATIAZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154351"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VLF19_11675"
     CDS             <1..1504
                     /locus_tag="VLF19_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE93957.1"
                     /translation="LLVEFPDHEQATAVRLALAWTVLRQGQRDAARQQFLEFARAHPT
                     NAHAVDALVLASELHLAGGDLPAARSLLDRIVADHGGHPRAELARFNRALLMLRAGQF
                     PAAQRELEAWITRAPFPALTGRAHAALGVALLGQGRAADAAREFARAQREGVGDLAGL
                     GLGSAALVERRWDEAARELTQARDAGTAPTAAAAEYGLAVVAFHRGRPAGFKDAARVT
                     LDAAPRGPAAPHLLYALTVVAADERDWPGGLALARRLVADFPQDEAADDGLERVGGGA
                     ATAGVWPVAYEAYALLRQRYPRSPFVESSRLAYGKALIETNRPQEARPVLEPLAAAAP
                     ADPRAAAESWLLLARAREAGGDARGALDAFSRAARGGDTAAWPKEALLAHARLLSAER
                     RWDEARAALDSVLRQADGAEAGETALAIGDTWARQGDAQAATEYYLTAVYLAPDSPAG
                     RRALLSAARTLATAKDRDGAATLYRKLLAQTGLPADIADEARRGLSDLKP"
     gene            1501..3318
                     /locus_tag="VLF19_11680"
     CDS             1501..3318
                     /locus_tag="VLF19_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HSE93958.1"
                     /translation="MKGYGGRILHVDLTRATSRVEPLHEAAARRLLGGNGLAARLLHD
                     LVPAGVDALDPANAAVFGVGPITDTTVPGNSRACVAAKSPLTGLFFDSTFGGRFPATL
                     KRTGFDAIAVTGRAAAPAYLLVTETGAELKSAAHLWGRTTRDTVQALVEAEGADADAL
                     AIGPAGERLVRFAAMAHYWKNREGVSGRGGLGAVLGSKNLKALVVRGARKTDIADPAA
                     LKALVERTREPLKTGTQALSTYGTPFLVGPINALGALGSYNLRSETFAQARAVGGDEM
                     KARFHDRDTTCLKCPVACGKQYAIADGEFAGTRAKMPEYETIFALGPMLGISHAEALI
                     LANDLCDLLGMDTISLGVTLAFVCEALERGWLKPDEVGVPFGWGDWRGMLRLVEMTAR
                     REGFGARLGEGAWRLAESVHPEATRLVYAVKRLELPAHSARALKGLSIGYATATRGGS
                     HHDTRPTPQYAQGFDRRSTADKPAFAIRSQHFTAIGDSLVMCRFTSERGFGLFLGEPY
                     AAMVRAVTGWDVTVDELERTGERIVNLERLFNVREGVRRAQDVLPWRVMHEPIPDGPS
                     AGMHCPPAELDAMLDDYYRLRGWDADGVPTPARLHALGL"
     gene            3473..4609
                     /locus_tag="VLF19_11685"
     CDS             3473..4609
                     /locus_tag="VLF19_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93959.1"
                     /translation="MQALAGPPTSWVLIGIIVASGVGAVLICLLVLRYGFPVRTWDGE
                     APPDRARGGTLLLLGHAAAAACFAIAAMLAVVALVHQYRASAALRAQGSGAGYAEVAE
                     VAEVQRALAAVEGRLARVEEHAAAADARLQAAAGRLESAQSRAQSADTRTTSLQSRLD
                     SSETRLAALEGRLQRAESSAHQAVGDATRALGGLKQVEQRMAATPPPARRPTVAEPAP
                     SATPSSSVSTRPAPSRPAPPRAERAPAPQASTPVRAGATPPREAGPAPAAQPSSGAEL
                     RAPVVTPPTGAPVVVAPAPDPTTAPKPLVRPSPSPQPMGQEAGGVPPPGQPAASARPA
                     PAPEPPQTFADRLRDDWESMKRDAETTNTELNKVWQKFRDFIGR"
BASE COUNT          654 a         1907 c         1805 g          615 t
ORIGIN      
        1 tctgctcgtc gagttcccgg accacgagca ggcgacggcc gtgcgcctgg ccctggcctg
       61 gacggtgctg cgccagggcc agcgcgacgc cgcgcgccag cagtttctcg agttcgcgag
      121 ggctcatccc acgaacgccc acgcggtgga cgccctggtg ctggcctccg agctgcacct
      181 ggccggcggc gaccttcctg ccgcccgctc gctgctcgac cggatcgtgg ccgatcacgg
      241 cggccacccc cgcgccgagc tggcgcgctt caaccgcgcg ctgctcatgc tgcgcgccgg
      301 gcaattcccc gccgcgcagc gcgagctgga ggcgtggatc acccgcgcac cgtttccggc
      361 cctcaccggg cgcgcccatg ccgcgcttgg cgtcgcactg ctcggccagg ggcgtgcggc
      421 ggacgccgcg cgcgagttcg cgcgggcgca gcgcgagggc gtcggtgacc tcgccgggct
      481 cggactgggc agcgcggctc tcgtcgagcg gcgctgggac gaggccgcgc gcgagctcac
      541 ccaggcgcgc gatgcgggca ccgcgccgac cgccgccgcc gccgagtacg gtctcgcggt
      601 ggtcgcgttc caccggggcc ggcccgcggg gttcaaggac gccgcacgcg tgacgctcga
      661 cgcggcgccg cgcggaccgg ccgcgccgca cctgctgtac gcgctcacgg tcgtggccgc
      721 cgacgagcgg gactggcccg gggggctggc gctcgccagg cggctcgtcg cggacttccc
      781 gcaagacgag gcggccgatg acgggctcga gcgagtggga ggcggcgcgg cgaccgccgg
      841 tgtctggccc gtcgcgtacg aggcctacgc gctgctccgc cagcgctacc cgcgcagccc
      901 gttcgtcgag agctcccggc tggcctacgg caaggcgctg atcgagacga accgcccgca
      961 ggaggccagg cccgtgctcg agccgctcgc ggcggcggca cccgccgatc cgcgcgccgc
     1021 cgcggaaagc tggctgctcc tggcacgcgc tcgcgaagcc ggcggtgacg cgcgcggcgc
     1081 gctcgacgcc ttctcgcggg ccgcgcgcgg cggtgacacc gccgcctggc cgaaggaggc
     1141 gctgctggcg cacgcgcgtc tgctgtcggc ggagcgccgc tgggacgagg cgcgcgccgc
     1201 gctcgacagc gtgctgcgcc aggccgacgg tgccgaggcc ggcgagaccg ccctcgcgat
     1261 cggcgacacc tgggcgcgcc agggtgacgc gcaggccgcg acggagtact acctgacggc
     1321 cgtctacctg gccccggact cgccggccgg gcgccgcgcc ctgctgtccg cggcccgaac
     1381 gctcgccacg gcgaaggatc gggacggcgc ggcgaccctc tatcgcaagc tcctcgcgca
     1441 gaccggcctg ccggccgaca tcgccgacga ggcgcgccgc gggctctccg acctcaagcc
     1501 gtgaagggct acggcggtcg catcctgcac gtcgatctga cccgcgccac gtcccgcgtg
     1561 gagccgctcc acgaggccgc cgcccgccgg ctcctgggcg gcaacgggct ggccgcgcgc
     1621 ctgctccacg acctggtgcc cgccggcgtg gacgccctcg atcccgccaa cgccgccgtc
     1681 ttcggcgtcg gcccgatcac ggacacgacg gtgcctggca acagccgtgc ctgcgtcgcc
     1741 gcgaagtcgc cgctgaccgg actcttcttc gactcgacct tcggcgggcg ctttccggcg
     1801 accctcaagc gcacgggttt cgacgcgatc gccgtcaccg gccgcgcggc ggcgccggcg
     1861 tacctgctcg tcaccgagac cggcgccgag ctgaagtcgg cggcccacct gtggggtcgg
     1921 acgacgcgcg acaccgtgca ggcgctcgtc gaggccgagg gcgccgacgc cgacgcgctg
     1981 gcgatcggcc cggccggcga gcggctcgtg cgcttcgccg ccatggcgca ctactggaag
     2041 aaccgcgagg gtgtctctgg ccgcggcggc ctcggtgccg tgctcggatc gaagaacctc
     2101 aaggctctcg tcgtgcgcgg cgcgcgcaag acggacatcg ccgatcccgc cgccctcaag
     2161 gcgctggtcg agcggacgcg cgagccgctc aagacgggca cgcaggcgct ctcgacgtac
     2221 ggcacgccgt tcctcgtggg ccccatcaac gcgctcggcg ccctcggcag ctacaacctg
     2281 agatccgaga cgttcgccca ggcgcgcgcc gtcggcggcg acgagatgaa ggcgcgcttc
     2341 cacgaccgcg acaccacgtg tctcaagtgc ccggtggcgt gcggcaagca gtacgcgatc
     2401 gccgacggcg agttcgccgg cacacgggcc aagatgccgg agtacgagac gatcttcgcc
     2461 ctcgggccga tgctcggcat cagccacgcc gaggccctga tcctcgccaa cgacctctgc
     2521 gacctgctcg gcatggacac gatcagcctc ggcgtcacgc tggccttcgt ctgcgaggcc
     2581 ctcgagcgtg gctggctgaa gccggacgag gtgggcgtgc cgttcgggtg gggcgactgg
     2641 cgcgggatgc tgcgcctggt cgagatgacg gcgcgccgcg agggcttcgg cgcccggctg
     2701 ggcgagggcg cctggcgcct ggccgagtcc gtccaccccg aggcgacgcg gctggtgtac
     2761 gccgtcaagc ggctcgagct gcccgcgcac tcggcgcgcg cgctcaaggg gctctccatc
     2821 ggctacgcca ccgccacgcg cggcggcagc caccacgaca cgcggcccac gccgcagtac
     2881 gcgcagggct tcgaccgccg gtcgaccgcc gacaagccgg cgttcgcgat ccgcagccag
     2941 cacttcacgg cgatcggcga ctcgctggtg atgtgccgct tcacctccga gcgcggcttc
     3001 gggctcttcc tcggcgagcc gtacgccgcg atggtgcggg ccgtcaccgg ctgggacgtc
     3061 accgtcgacg agctcgagcg cacgggcgag cgcatcgtca acctcgagcg actcttcaac
     3121 gtccgcgagg gcgtgcgccg cgcgcaggac gtgctgccgt ggcgcgtgat gcacgagccg
     3181 atccccgacg gcccctcggc gggcatgcat tgcccgccgg ccgagctcga cgcgatgctc
     3241 gacgactact atcgcctgcg gggctgggac gccgacggag tccccacgcc cgcccgcctg
     3301 cacgcgctcg ggctgtagcc gccgcacgcc acggtcggcc acgccgtgct cggccacgcc
     3361 acggccggcc acgccgcggt cggcccgcag cggtcggccc gggtactctc ccgcaagccg
     3421 accctcttcc agaccctgat acagtaggag gcaggggcaa gaagggaggc gtatgcaggc
     3481 cctcgccggt cccccgactt cctgggtgct catcggcatc atcgtcgcca gcggcgtcgg
     3541 cgcggtgctc atctgcctgc tggtgctccg ctacggattt cccgtgcgca cctgggacgg
     3601 ggaggcgcct cctgatcgcg cccggggcgg aaccctgctg ctcctggggc acgctgcggc
     3661 cgcggcctgc ttcgcgatcg ccgccatgct cgcggtcgtc gccctcgtgc accagtatcg
     3721 tgcgtccgct gcgctccgcg cccagggctc cggggccggg tacgccgagg tcgccgaagt
     3781 cgccgaggtc cagcgggcac tcgcggccgt cgaggggcgg ctggccagag tcgaggagca
     3841 cgccgctgca gcggatgccc gcctccaggc ggccgccgga cgtctcgagt ccgcgcagtc
     3901 gcgcgcgcag tccgccgaca cgcgcaccac ctcgctgcag tcgcggctgg actcgtcgga
     3961 gaccaggctg gcagcactcg agggccggtt gcagcgcgcg gagtcgtcgg cgcatcaggc
     4021 cgtcggcgac gccacacgcg cgctgggggg actcaagcag gtcgagcaac ggatggccgc
     4081 aacaccacct cccgcacgcc gtcccacggt ggccgagccg gcgccgtcgg ccacgccgtc
     4141 ctcctccgtg tcgactcgcc cggcgccgtc acgcccggcg ccgccacgcg cggaacgggc
     4201 gcccgcgccg caggcgagca cgccggtgcg agcgggggcg acgccgccac gtgaggccgg
     4261 tccagcgccg gccgcgcagc cttcgtcggg cgccgagctt cgcgcgcccg tcgtcactcc
     4321 gcccacgggc gcaccggtgg tcgtggcgcc ggcacccgac ccgacgacgg caccgaagcc
     4381 gctcgtccgg ccgtcgccga gccctcagcc catgggccag gaagccggcg gcgtcccgcc
     4441 tccagggcag ccggccgcca gcgcgcggcc ggcgccggcc ccggagccgc cgcagacgtt
     4501 cgccgaccgg ctccgggacg actgggagtc gatgaagcgt gacgccgaaa cgacgaatac
     4561 cgagctgaac aaggtctggc agaagttccg cgacttcatc ggccgctgag gcatcgtgat
     4621 ccgctgaccc tgcccggcca cgcgggcgga tagctcgctc ggacctcgac agagaccggc
     4681 ccatcggacg gactcgcgcg ctcgctgccg cgatacgtcg actgattgta gcgatggagc
     4741 aaacaacgtg acgcgcgccc gctcaggtaa cgtcggagag ggtgtaaacg agtgagggcg
     4801 gtgtaacctt cggttcgcgt caagaacctc ctgcgcgtca acgcccgaac agaaggagac
     4861 accgcctgtg agagagaaga ccatcgaaac gggtcgcggc gtcaagcccg acctgggcga
     4921 cgctggagac gttcgcgcgc gagaacatgc agcagttcct gcagcagctg ttggtggagg
     4981 a
//
LOCUS       DATIAZ010000342        11092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1552584, whole genome shotgun sequence.
ACCESSION   DATIAZ010000342 DATIAZ010000000
VERSION     DATIAZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552584"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VLF19_11690"
     CDS             complement(<1..174)
                     /locus_tag="VLF19_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HSE93960.1"
                     /translation="MGTPSRLVFLGPPGAGKGTQARELAQEWGVPQIATGDMLREAMA
                     ADTPLGREAKQYYD"
     gene            complement(180..1508)
                     /gene="secY"
                     /locus_tag="VLF19_11695"
     CDS             complement(180..1508)
                     /gene="secY"
                     /locus_tag="VLF19_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSE93961.1"
                     /translation="MIEGLQSFQNVFKIPELKRRLLFTGGLLIAYRLGAHVPTPGIDG
                     HALAQFFDQMQQGLLGMVDLFSGGNLRRLSIFALGIMPYISASIILQLLTVVVPTLER
                     LAKEGEAGRKKITQYTRYGTIVLSAVQSLGIAYGLERMPTPTGATIVPYPGWGFRLLT
                     MITLTAGTALIMWIGEQISEKGIGNGISLIIFAGIVVRLPSAIVASYTLITTGEMKLF
                     VFIALCLLMLAVTAAVVLMQEGQRKIPVQYARRVVGRRVYGGQSTHIPLRINTAGVIP
                     VIFASSLILFPATLTNFIPHPWMRVMSDALSPGRFTYTVLYMALIVFFAYFYTAIVFN
                     PVDLADNMKKYGGFIPGVRPGKKTAEYIDHVLTRITLPGALFLAIISVLPDFLIRWFN
                     VPFYFGGTSLLIVVGVALDTVRQMESHLLMRNYEGFLKKSRSRAGAFGRT"
     gene            complement(1505..1972)
                     /gene="rplO"
                     /locus_tag="VLF19_11700"
     CDS             complement(1505..1972)
                     /gene="rplO"
                     /locus_tag="VLF19_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HSE93962.1"
                     /translation="MKLDDLRPAPGARTRRKRVGRGPGSGHGKTSGKGHKGHKARSGG
                     GKTGGFEGGQMPLYRRLPKRGFLPHGGKDPYAIVNLRSLGGFAADAVVDPDALVAAGL
                     VKRRDRERVKILGAGDLPHALTVKVHAISESARAKVVERGGRVELLAHAVVAS"
     gene            complement(1962..2150)
                     /gene="rpmD"
                     /locus_tag="VLF19_11705"
     CDS             complement(1962..2150)
                     /gene="rpmD"
                     /locus_tag="VLF19_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HSE93963.1"
                     /translation="MASKKLKVTLRRSIIGLSPKQEATVRALGLRRMNQSVVHADTAT
                     IRGMIAKVPHVLQVGHEA"
     gene            complement(2131..2667)
                     /gene="rpsE"
                     /locus_tag="VLF19_11710"
     CDS             complement(2131..2667)
                     /gene="rpsE"
                     /locus_tag="VLF19_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSE93964.1"
                     /translation="MAEARIDPSVLELNDRVVSINRVAKVVKGGRRFSFTALVVVGDG
                     RGHVGVGLGKAHEVPEAIRKAVEHAKKDLIFVPLRETTIPCEITGHFGAGRVFLKPAV
                     PGTGVIAGGAVRPVLEAVGVQDILTKTLGTNNPHNVLKATMDALRRIRRIQDLHAARK
                     RSDGNNAGEEAVVGKQEA"
     gene            complement(2740..3105)
                     /gene="rplR"
                     /locus_tag="VLF19_11715"
     CDS             complement(2740..3105)
                     /gene="rplR"
                     /locus_tag="VLF19_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSE93965.1"
                     /translation="MIIKDRRAQRDLRHERIRRTVRGTATRPRLAVFRSLNHIYAQLV
                     DDDTGRTLVAADSRSKDFRAQHKSGGNVAAAKAVGTLLAERAKAHGLDRAVFDRGGYM
                     YHGRVKALADAARAGGLVF"
     gene            complement(3102..3644)
                     /gene="rplF"
                     /locus_tag="VLF19_11720"
     CDS             complement(3102..3644)
                     /gene="rplF"
                     /locus_tag="VLF19_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSE93966.1"
                     /translation="MSRIGKKPIVIPQGVKVQVDGPVVRAEGPKGKLLQPVPRGLTAK
                     VDDGHLVLTREGDHRAIRALHGLTRALVANMVHGVKDGFERKLEIVGIGYRAQLQGRN
                     IQLALGYSHPVIFPLPEGVTAEIDKQVSITLRGPDKAVLGQTAAKLRALRKPDPYKGK
                     GIRYADEVIRRKVGKKAGAK"
     gene            complement(3655..4053)
                     /gene="rpsH"
                     /locus_tag="VLF19_11725"
     CDS             complement(3655..4053)
                     /gene="rpsH"
                     /locus_tag="VLF19_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSE93967.1"
                     /translation="MRSDPIADMLTRVRNASRAEHEKVDIPSSKLKVRVAEILKNEGF
                     IKNYRVLEDDKKQGTLRVYLKYGPGNEQMISGLVRVSSPGRRVYVGHDRIPSILAGMG
                     VTIVSTSRGVVTDRDARKQKIGGEVLAYVW"
     gene            complement(4061..4246)
                     /locus_tag="VLF19_11730"
     CDS             complement(4061..4246)
                     /locus_tag="VLF19_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HSE93968.1"
                     /translation="MAKTALIMKSMETPKFRTRAYSRCKLCGRPRGYLRKFQMCRLCF
                     RELALKGEVPGVVKASW"
     gene            complement(4256..4798)
                     /gene="rplE"
                     /locus_tag="VLF19_11735"
     CDS             complement(4256..4798)
                     /gene="rplE"
                     /locus_tag="VLF19_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSE93969.1"
                     /translation="MAPRLRDRFRTTVVQALMKERNYGNPFEVPRLEKIVINMGVGDA
                     RDNAKVMDFATADLQAITGQKPIVTRARKSIANFKLREGVAIGAKVTLRGARMYEFLD
                     RLVNVALPRVRDFKGVPPKGFDGRGNYALGLREQLIFPEIVYDKVDKVRGMDIVIVTT
                     ARSDEDAKALLTHLGMPFRE"
     gene            complement(4945..5271)
                     /gene="rplX"
                     /locus_tag="VLF19_11740"
     CDS             complement(4945..5271)
                     /gene="rplX"
                     /locus_tag="VLF19_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSE93970.1"
                     /translation="MAGAHVRRGDTVAVIAGRERGKRGRVLRVLGDRARVVIEKVNMV
                     KKHQRPTQKLRQGGIIEREGALALSNVLLVCSRCDRPVRTGVKVLADGRKIRACKRCG
                     EPVDRG"
     gene            complement(5271..5639)
                     /gene="rplN"
                     /locus_tag="VLF19_11745"
     CDS             complement(5271..5639)
                     /gene="rplN"
                     /locus_tag="VLF19_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSE93971.1"
                     /translation="MIQPRSMLEVADNSGAKKVQCIRVMGGANKRYASLGDTVMVAVK
                     EAAPDGTVKKGEVARAVVVRTVKEVRRKDGSYIRFDRNAVVLIKPDENPVGTRIFGPV
                     ARELRERQFTKIISLAPEVI"
     gene            complement(5643..5900)
                     /gene="rpsQ"
                     /locus_tag="VLF19_11750"
     CDS             complement(5643..5900)
                     /gene="rpsQ"
                     /locus_tag="VLF19_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSE93972.1"
                     /translation="MRTVGEQKTREGVVVSDKMEKTRVVRIERVYRHPRYQRVVRRAK
                     RLKAHDERNESRIGDRVVIEETRPISKDKRWRIREIVSRVS"
     gene            complement(5897..6271)
                     /gene="rpmC"
                     /locus_tag="VLF19_11755"
     CDS             complement(5897..6271)
                     /gene="rpmC"
                     /locus_tag="VLF19_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSE93973.1"
                     /translation="MKAAKWRDLSEDEVRQKMRELTEEVFNLRFQLSMGVTKNPSRLS
                     QARRDLARGTTILAERGGSPRSGNRPSAPGESPAVGRTVAEPTESPAVGRRKLGGRTS
                     RAGSPTARRGARTANTKTKKRG"
     gene            complement(6306..6722)
                     /gene="rplP"
                     /locus_tag="VLF19_11760"
     CDS             complement(6306..6722)
                     /gene="rplP"
                     /locus_tag="VLF19_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSE93974.1"
                     /translation="MLMPKRVKYRKAQRGRMRGKAQRGSALAFGEYGLKALEPGWVTN
                     RQIEAARVALTRSLKRGGKIWIRIFPDKPVTKKPAETRMGKGKGNPEDWVAVVKPGRI
                     LYEMEGVPEDAAREAFRTAGQKMGIATRFVARVRTL"
     gene            complement(6733..7377)
                     /gene="rpsC"
                     /locus_tag="VLF19_11765"
     CDS             complement(6733..7377)
                     /gene="rpsC"
                     /locus_tag="VLF19_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSE93975.1"
                     /translation="MGQKTHPIGFRLGTTRTWSSRWFATKDYAKLLHEDVKIRRYIKD
                     QLYHAGIARIDIERSANRARITVATARPGIIIGRKGAEVERLKNELQQRTGKEIYLNI
                     EEVIHPELDAQLVAENVALQLQKRVAFRRAMKKAVTSSLRLGADGVRIACAGRLGGSE
                     IARREWYRDGRVPLHTLRADIDYGLATAHTTYGTIGVKVWIFKGEVLAKTPAEA"
     gene            complement(7684..8025)
                     /gene="rplV"
                     /locus_tag="VLF19_11770"
                     /pseudo
     CDS             complement(7684..8025)
                     /gene="rplV"
                     /locus_tag="VLF19_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(8028..8321)
                     /gene="rpsS"
                     /locus_tag="VLF19_11775"
     CDS             complement(8028..8321)
                     /gene="rpsS"
                     /locus_tag="VLF19_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSE93976.1"
                     /translation="MGRSLKKGPYVDETLLGRVEEMNRSRQKKVLKTWSRRSTILPEF
                     VGHTLAVHNGRKFIPVYVTENMVGHRLGEFALTRTFRAHGTAEKASASPTGKA"
     gene            complement(8333..9157)
                     /gene="rplB"
                     /locus_tag="VLF19_11780"
     CDS             complement(8333..9157)
                     /gene="rplB"
                     /locus_tag="VLF19_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HSE93977.1"
                     /translation="MGIRTLKPTSAARRYLTYVTTEELTRKEPEKSLLVPKTRISGRN
                     TYGRITVRHRGGGAKRMLRQVDFRRDKVGIPARVAAIEYDPNRSARLALLHYRDGEKR
                     YIVAPLGLKAGDVVMSGPQADILPGNALPLRNIPVGTLVHNVELQEGRGGQLCRSAGA
                     QAQLLAKEGDRATLKLPSGEVRYVAVACMATIGQVGNLDHENVSVGKAGRVRWMGFRP
                     TVRGTVMNPVDHPMGGGEGKGKGNHPMTPWGKPTKGYKTRRGARPSDSSIVTRRKK"
     gene            complement(9162..9455)
                     /gene="rplW"
                     /locus_tag="VLF19_11785"
     CDS             complement(9162..9455)
                     /gene="rplW"
                     /locus_tag="VLF19_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSE93978.1"
                     /translation="MTRSAREIILRPIMTEKSMRQKEEQNVVTFQVRPDANKVEIRQA
                     VEAVFNVKVADVRTASYEGKLKRMGRHQGRRPDWKKAVVALKPGHKIELVEGA"
     gene            complement(9452..10081)
                     /gene="rplD"
                     /locus_tag="VLF19_11790"
     CDS             complement(9452..10081)
                     /gene="rplD"
                     /locus_tag="VLF19_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSE93979.1"
                     /translation="MPTVQVLGDKGKPTGTLELKADVFGVEIRVPLLHQAIVRELADR
                     RAGTHDTKGRSEVSGGGKKPWKQKGTGRARQGSIRATQWKGGGKPFGPTPRSYAQVLP
                     RQMRRGALRAAVAARIADGALKAVETLGAADGKTRSLVAWFAEVGLAEVPTVLVVREV
                     GEPLARAARNVPWLTVETPAHVSAYQLLRVRQAVFERGALLALEEALAP"
     gene            complement(10081..10767)
                     /gene="rplC"
                     /locus_tag="VLF19_11795"
     CDS             complement(10081..10767)
                     /gene="rplC"
                     /locus_tag="VLF19_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSE93980.1"
                     /translation="MARKEGLIGRKVGMTQVFGDDGSQIPVTVIQAGPCPVVGIRTRE
                     SHGYDALQLGFEAMRKNVTKPMAGHFKKAGVGAVRVLRELRLDKATAEKLGGVQVGQA
                     LTVEMFAPGELVDVVGVTKGKGFQGGVRRHGWYGGDATHGSMFHRAPGSIGASSDPSR
                     VWPGHALPGRMGGERRTVLNVSVVRVLPEQHLVLVRGAVPGASGSLVMVRKSVKTTKA
                     QQQKATADRK"
     gene            complement(10901..>11092)
                     /gene="rpsJ"
                     /locus_tag="VLF19_11800"
     CDS             complement(10901..>11092)
                     /gene="rpsJ"
                     /locus_tag="VLF19_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011643734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSE93981.1"
                     /translation="VLLPTIVNRWTVLRSPHVDKTSREQFEMRTHKRLLDIVDPTPQT
                     VDSLMKLDLPAGVDVEIKL"
BASE COUNT         1450 a         4075 c         3696 g         1871 t
ORIGIN      
        1 atcgtagtac tgcttggcct cgcggccgag cggcgtgtcg gcggccatgg cctcgcgcag
       61 catgtcaccc gtggcgatct gcggcacacc ccactcctgg gcgagttcac gggcctgggt
      121 gcccttgccg gcgccgggcg ggccgaggaa gaccagacgg ctcggcgttc ccaccgccct
      181 caggtcctcc cgaaggcccc cgcccgcgag cgcgacttct tgaggaagcc ctcgtagttg
      241 cgcatgagca ggtgggactc catctggcgc acggtgtcga gggccacgcc gacgacgatc
      301 aggaggctgg tgccgccgaa gtagaacggc acgttgaacc atcggatcag gaagtccggg
      361 agcacggaga tgatcgccag gaacagcgcc cccggcaggg tgattcgcgt gaggacgtgg
      421 tcgatgtact ccgccgtctt cttgcccggc cgcacgccgg gaatgaagcc gccgtacttc
      481 ttcatgttgt cggcgaggtc gacgggattg aacacgatgg ccgtgtagaa gtaggcgaag
      541 aagacgatca gcgccatgta gagcacggtg taggtgaagc gccccgggct gagcgcgtcg
      601 gacatcaccc gcatccacgg gtgtgggatg aagttcgtga gcgtggccgg gaacaggatg
      661 agcgacgagg cgaagatcac ggggatgacg ccggcggtgt tgatgcgcag cgggatgtgg
      721 gtggactgcc cgccgtacac ccgccggccg accacgcggc gcgcgtactg cacgggaatc
      781 ttgcgctggc cctcctgcat caggacgacc gccgccgtca ccgcgagcat cagcaggcac
      841 agcgcgatga acacgaacag cttcatctcg ccggtcgtga tcagcgtgta ggaagcgacg
      901 attgccgagg gcagccgcac gacgatgccc gcgaagatga tgagcgagat gccgttcccg
      961 atgcccttct cggagatctg ctcgccgatc cacatgatca gcgccgtgcc cgccgtcagc
     1021 gtgatcatcg tcaggagacg gaacccccag ccggggtacg gcacgatggt ggcgcccgtc
     1081 ggcgtcggca tgcgctcgag cccgtaggcg atgcccaacg actgcaccgc cgacaggacg
     1141 atcgtgccgt agcgggtgta ctgggtgatc ttctttcgcc cggcctcgcc ctccttggcc
     1201 agccgctcga gcgttggcac gaccacggtg aggagctgca ggatgatcga cgcggagatg
     1261 tagggcatga tgccgagggc gaagatcgac aggcggcgga ggttcccgcc ggagaacaga
     1321 tcgaccatgc cgagcaggcc ctgctgcatc tgatcgaaga actgggccag ggcatggccg
     1381 tcgatgcccg gcgtgggcac gtgggcgccc aggcgatagg cgatgagcag gccccccgtg
     1441 aacagcaggc ggcgcttcag ctcaggaatc ttgaagacgt tctggaagct ctggaggcct
     1501 tcgatcacga ggccaccaca gcgtgcgcca gcagctcgac gcggccgccc cgctcgacga
     1561 ccttcgcgcg cgccgattcg ctgatcgcgt gcaccttcac cgtgagcgcg tggggcagat
     1621 cgcccgcacc gagaatcttc acgcgctccc ggtcgcgccg cttcacgagg ccggcggcga
     1681 cgagcgcgtc gggatcgacc acggcgtcgg cggcgaagcc tccgagcgat ctcagattca
     1741 cgatcgcata ggggtccttg cccccgtggg gcaggaagcc gcgcttgggc agccgccggt
     1801 acagcggcat ctgcccgcct tcgaagccgc ccgtcttgcc gccgccggag cgggccttgt
     1861 ggcccttgtg gcccttgccc gacgtcttgc cgtggcccga gccgggaccg cggcccaccc
     1921 gcttgcgccg cgtcctggcg cccggcgccg ggcgcaggtc gtcaagcttc atggcccacc
     1981 tggagcacgt gcgggacctt ggcgatcatt ccgcggatgg tcgccgtatc ggcgtgcacg
     2041 accgactggt tcatgcggcg cagccccagc gccctgaccg tcgcctcctg cttcggggac
     2101 aggccgatga tgctgcgcct gagcgtcacc ttaagcttct tgcttgccaa cgactgcctc
     2161 ctcgcccgcg ttgttgccgt cgctgcgctt gcgcgccgcg tgcaggtcct ggatgcgccg
     2221 gatccgccgg agcgcgtcca tggtggcctt cagcacgttg tgcggattgt tcgtgcccag
     2281 cgtcttggtg agaatgtcct gcacgccgac ggcctcgagg accggccgga cggcgccgcc
     2341 cgcgatgacg ccggtgccgg gcaccgctgg cttcaggaag acccggccgg ccccgaagtg
     2401 gccggtgatc tcgcagggaa tcgtcgtctc cctgagcggc acgaagatga ggtccttctt
     2461 ggcgtgctcg accgccttgc ggatggcctc gggcacctcg tgggccttgc cgaggccgac
     2521 gccgacgtgg ccgcggccgt ccccgaccac gacgagcgcg gtgaacgaga agcgccggcc
     2581 gcccttgacg accttggcca cccggttgat ggacaccacc cggtcgttga gctcgagcac
     2641 actcggatcg atcctcgctt cggccactca gcgtctcctc gtcgtccctc gccgacccac
     2701 gttctctccc tcgcctcgtt cgcgcggcgg ctttccgctc tagaagacca ggccgccggc
     2761 gcgtgccgcg tcggcgagcg ccttcacgcg cccgtggtac atgtagcccc cccggtcgaa
     2821 cacggcccga tcgaggccgt gcgccttcgc gcgctcggcg agcagcgtgc ccacggcctt
     2881 cgcggccgcg acgttgccgc cgctcttgtg ctgcgcccgg aagtccttgc tccggctgtc
     2941 ggcggccacc agcgtgcggc cggtgtcgtc gtcgacgagc tgggcgtaga tgtggttcag
     3001 gctccggaac acggccagcc gcggccgcgt cgccgtgccg cgcacggtcc gacggatgcg
     3061 ttcgtgccgg aggtcgcgct gcgcccgccg gtccttgatg atcatttcgc gcccgccttc
     3121 ttgccgacct tgcgccggat gacctcgtcg gcgtacctga tgcccttgcc cttgtaggga
     3181 tcgggcttgc gcagcgcccg cagcttcgcc gccgtctggc cgagcacggc cttgtccggg
     3241 ccgcgcagcg tgatcgacac ctgcttgtcg atctccgcgg tgaccccctc gggcagcggg
     3301 aagatcacgg ggtgcgagta cccgagggcg agctggatgt tccggccctg gagctgcgcg
     3361 cggtagccga taccgacgat ctcgagcttg cgctcgaatc cgtccttcac gccgtgcacc
     3421 atgttggcca cgagcgcgcg cgtgaggccg tgcaacgcgc gaatcgcgcg gtggtcgccc
     3481 tcgcgggtga gcacgaggtg gccgtcgtcg accttcgcgg tgaggccccg gggcaccggc
     3541 tgcagcagct tgcccttggg gccctcggcg cggacgaccg ggccgtccac ctggaccttc
     3601 acgccctgcg ggatgacgat gggtttcttg ccgatccgtg acatcgcgag ccccctacca
     3661 cacgtaggcc agcacttcgc cgccgatctt ctgcttgcgc gcgtcccggt ccgtcaccac
     3721 accgcgcgag gtcgagacga tcgtcacacc catgccggcc aggatcgagg ggatccggtc
     3781 gtgccccacg tacacccgcc gccccggcga ggacacgcgc accaggcccg agatcatctg
     3841 ctcgttgccg ggtccgtact tgagatacac gcgcagcgtc ccctgcttct tgtcgtcctc
     3901 cagcacgcgg tagttcttga tgaagccctc gttcttgagg atctcggcca cgcgcacctt
     3961 gagcttcgac gacgggatgt cgaccttctc gtgctccgcc cggctcgcgt tgcggacgcg
     4021 cgtcagcatg tcggcgatcg gatcactcct catggccgcc tcaccagctc gccttgacca
     4081 cgccgggcac ctcgcccttg agggccagct cccggaagca caggcgacac atctggaact
     4141 tccgcagata gccgcgcgga cgcccgcaga gcttgcaacg ggagtacgcg cgggtccgga
     4201 acttcggagt ttccatggac ttcatgatca gtgcggtctt ggccatgacg acaatctact
     4261 ccctgaaggg catgcccagg tgcgtgagga gcgccttggc gtcctcgtcg gacctggccg
     4321 tggtgacgat gacgatgtcc atgccgcgga ccttgtcgac cttgtcgtag acgatctcgg
     4381 ggaagatcag ctgctcccgg aggccgaggg cgtagttgcc gcgcccgtcg aagcctttcg
     4441 gcggcacgcc cttgaagtcg cgcacccgcg gcagcgcgac gttgaccagc cggtccagga
     4501 actcgtacat gcgcgccccg cggagcgtca ccttggcgcc gatggccacg ccctcgcgga
     4561 gcttgaagtt cgcgatcgac ttccgcgcgc gggtcacgat gggcttctgg ccggtgatgg
     4621 cctgcaggtc ggccgtcgcg aagtccatca ccttggcgtt gtcgcgggcg tcgcccacgc
     4681 ccatgttgat cacgatcttc tccaggcgcg gcacctcgaa ggggtttccg tagttccgct
     4741 ccttcatgag cgcctggacg accgtcgtcc ggaagcggtc acgcagccgg ggcgccacct
     4801 gcccggtgcc cttcggccgc gtctttgccg ccggggccgc cgccggcgcg ccgtcgccct
     4861 tccggggctg cggcttgccg gccggagcct tcgccccctg gggcttctgc gccggtgctc
     4921 ctgtccctgg cttggccatg gctcctaacc cctgtccacg ggctcgccgc accgcttgca
     4981 cgcgcggatc ttgcggccgt cggcgagcac cttcacgccg gtgcgcacgg gccggtcgca
     5041 ccgcgagcag acgagcagca cgttcgacag cgccagcgcg ccctcgcgct cgatgatgcc
     5101 gccctgccgg agcttttgcg tgggccgctg gtgcttcttg accatgttga ccttctcgat
     5161 caccacgcgg gcccggtcgc cgagcacgcg gagcaccctg ccccgcttgc cgcgctcgcg
     5221 gcccgcgatg acggccaccg tgtcgcccct ccgcacgtgc gcgcccgcca tcagatgacc
     5281 tccggcgcca gcgagatgat cttggtgaac tgccgttcgc gcagctcgcg agcgacgggc
     5341 ccgaagatcc gggtcccgac ggggttctcg tcgggcttga tgagcaccac cgcgttgcgg
     5401 tcgaaccgga tgtaggagcc gtccttgcgg cggacctcct tcaccgtgcg caccaccacc
     5461 gcgcgcgcca cctcgccctt cttgacggtc ccgtccggcg cggcctcctt gacggcgacc
     5521 atgacggtgt ccccgagcga ggcgtagcgc ttgttggcgc cgcccatgac ccggatgcac
     5581 tgcaccttct tggcgccgga gttgtcggcg acctcgagca tcgagcgtgg ctggatcatc
     5641 gctcaggaca cccggctcac gatctcgcgg atccgccagc gcttgtcctt gctgatcggg
     5701 cgcgtctcct cgatgaccac gcggtcgccg atccggctct cgttccgctc gtcgtgcgcc
     5761 ttcagccgct tcgccctgcg cacgacgcgc tggtagcgcg ggtggcggta gacccgctcg
     5821 atgcgcacca cgcgcgtctt ctccatcttg tcgctgacga cgaccccttc acgggtcttc
     5881 tgctcgccca cggtcctcag cctcgtttct tggtcttcgt attcgccgtc cgggcgccgc
     5941 gccgggcggt cggcgacccg gcgcgggagg tccgtccgcc gagcttccgg cgcccgacgg
     6001 ccggcgactc ggtgggctcg gccaccgtgc ggccgacggc cggcgactcc ccgggcgcgg
     6061 acggccggtt cccggagcgc ggcgaaccgc cgcgctcggc gaggatggtc gtcccccgcg
     6121 ccaggtcccg gcgcgcctgc gacagacgcg acgggttctt ggtcacgccc atcgagagct
     6181 ggaaccggag gttgaagacc tcttcggtca gctcgcgcat cttctgccgc acctcgtcct
     6241 cgctcagatc gcgccacttg gcggccttca tcgtgccctc gccggaccct cgtgtcccct
     6301 caccgctaca gcgtccggac gcgcgcgacg aaccgcgtcg cgatgcccat cttctgcccc
     6361 gccgtccgga acgcctcgcg cgccgcgtcc tccggcacgc cttccatctc gtagaggatc
     6421 cggccgggct tgaccacggc gacccagtcc tcggggttgc ccttgccctt gcccatccgg
     6481 gtctcggccg gcttcttcgt caccggcttg tccgggaaga tccggatcca gatcttgcct
     6541 ccccgcttga ggctgcgcgt gagcgcgacg cgcgccgcct cgatctggcg gttcgtgacc
     6601 cagcccggct cgagagcctt gagcccgtac tcgccgaagg ccagcgctga cccgcgctgc
     6661 gccttgccgc gcatgcggcc ccgctgggcc ttgcgatact tgacgcgctt gggcatcagc
     6721 atcggggaat ccctacgcct ccgccggcgt cttggccagc acttcgccct tgaagatcca
     6781 caccttcacg ccgatcgtgc cgtacgtcgt gtgggcggtg gccaggccgt agtcgatgtc
     6841 ggcccgcagc gtgtgcagcg gcacgcgccc gtcgcggtac cactcgcggc gcgcgatctc
     6901 gctgccgccc agccgccccg cgcacgcgat gcgcacgccg tcggccccca ggcgcagcga
     6961 ggaggtgacc gccttcttca tcgcccgccg gaacgccacg cgcttctgca gctgcagcgc
     7021 cacgttctcg gctacgagct gcgcgtcgag ttcggggtgg atgacctcct cgatgttgag
     7081 gtagatctcc ttccccgtcc gctgttgcag ctcgttcttg agacgctcga cctcggcgcc
     7141 cttgcggccg atgatgatgc ccggacgcgc cgtggccacc gtgatgcgcg cgcggttcgc
     7201 cgagcgctcg atgtcgatgc gcgcgatgcc cgcgtggtag agctggtcct tgatgtagcg
     7261 gcggatcttc acgtcctcgt ggaggagctt cgcgtagtcc ttggtcgcga accatcgcga
     7321 cgaccacgtg cgtgtcgtcc ccagccggaa cccgatcgga tgcgtcttct ggcccatcgc
     7381 catttctcgg aggagggcta cgcccccctt ccgagcctcc cccgagcacc tcggcggggg
     7441 gcggcgcccc ccttccgaac cgcgcccctt cgatggcgcc ggcatagccg gcgccggatc
     7501 tcgcgccaga tcatctcagc ccgtccttct ccgagacgta ccccgacccc gccccttggc
     7561 cccggccgac gaccgcgccc tggcccccgt cccgactctg cgcgccctcg gcgcctccgt
     7621 cgtcgtcgcg ggctccggtg ccgcgcggcg acgcgcccgt cgcggagcct gcggcggcgg
     7681 cgcgccgttg gtctcgtcgc tcacgccgac cgtgaggtga ctcgtccggt gccggatgaa
     7741 gaaggcccgt cccatcgccc gcggctgcac gcgcttgagc gagggcccgg cgtcggccgt
     7801 cgccctcacg acgcggagca cgtcgaggtc gcgcacctgg tggttgtgct cggcgttggc
     7861 caccgccgag cgcagcacct tctcgatgag ccgcgccgcc gcgcgcggcg tgtacttcaa
     7921 gatcgccagc gcctcgccca cgggcttgcc gcggatctgg cccaggacga ggcccgcctt
     7981 gcggggcgag acccggacga agcgggccgt ggcctgcgtc agcatcgcta ggccttgccc
     8041 gtgggcgagg ccgaggcctt ctcggccgtg ccgtgcgccc tgaacgtccg cgtcagcgcg
     8101 aactcgccga gccggtggcc caccatgttc tcggtgacgt agacgggaat gaacttcctg
     8161 ccgttgtgga cggccagtgt gtgtcccacg aactcgggca ggatggtcga gcgccgcgac
     8221 caggtcttga gcaccttctt ctgacgcgag cggttcatct cctcgacgcg cccgaggagc
     8281 gtctcgtcca cgtacggtcc cttcttgagt gagcgtccca tcgccggctc ccctatttct
     8341 tccgtcgcgt cacgatggac gagtccgacg gccgcgcgcc ccgacgcgtc ttgtatccct
     8401 tggtgggctt gccccacggc gtcatcggat ggttgccctt gcccttgccc tcgccgccgc
     8461 ccatcgggtg gtcgacgggg ttcatgacgg tgccgcggac ggtcggacgg aagcccatcc
     8521 accgcacgcg gccggccttg ccgaccgaga cgttctcgtg atcgaggttg ccgacctggc
     8581 cgatggtggc catgcacgcg acggccacgt agcgcacctc gcccgacggc agcttgagcg
     8641 tcgcgcggtc gccctccttg gccagcagct gggcctgcgc ccccgcgctg cggcagagct
     8701 gtccgccgcg gccctcctgc agctccacgt tgtgcacgag ggtgcccacg gggatgttgc
     8761 gcagcggcag cgcgttgccg ggcaggatgt cggcctgcgg gccggacatg acgacgtcgc
     8821 cggccttgag ccccagcggc gcgacgatgt agcgcttctc gccgtcgcgg tagtgcagca
     8881 gcgccaggcg cgccgagcgg ttcgggtcgt actcgatcgc ggccacccgc gccgggatgc
     8941 cgaccttgtc gcgccggaag tcgacctgac gcagcatgcg cttggccccg ccgccccggt
     9001 ggcgcacggt gatgcggccg taggtgttgc ggccgctgat gcgcgtcttc gggacgagca
     9061 gcgacttctc gggctccttc cgcgtcagct cctcggtggt gacgtaggtc aggtagcgac
     9121 gcgcggccga cgtcggcttc agggtgcgga tgcccatggc ctcaggctcc ctccacgagc
     9181 tcgatcttgt gcccgggctt gagcgccacg accgccttct tccagtcggg ccgccgcccc
     9241 tgatggcggc ccatccgctt cagcttgccc tcgtacgacg ccgtgcggac gtcggcgacc
     9301 ttgacgttga agacggcctc cacggcctgg cggatctcca ccttgttggc gtcgggccgc
     9361 acctggaagg tgaccacgtt ctgctcttcc ttctgccgca tgctcttctc ggtcatgatc
     9421 ggccgcagga tgatctcgcg cgcgcttcgg gtcatggggc cagcgcctcc tcgagcgcga
     9481 gcagcgcgcc gcgctcgaac accgcctggc gcacacgcag gagctggtag gccgacacgt
     9541 gggccggcgt ctccacggtc agccagggca cgttgcgggc cgcgcgcgcg aggggctcgc
     9601 ccacctcgcg cacgaccagc accgtcggca cctccgcgag accgacctcg gcgaaccacg
     9661 ccaccagcga cctggtcttg ccgtccgcgg cgccgagcgt ctcgacggcc ttgagggcgc
     9721 cgtcggcgat ccgcgccgcc accgccgcgc gcagcgcacc gcgccgcatc tggcgcggca
     9781 ggacctgcgc gtaggaccgc ggcgtgggac cgaacggctt gccgccgccc ttccactgcg
     9841 tggcgcggat gctgccctgg cgcgcgcgcc ccgtgccctt ctgcttccac ggcttcttgc
     9901 cgccgccgga cacctcgctg cgccccttgg tgtcgtgggt cccggcacgg cggtcggcca
     9961 gctcgcggac gattgcctgg tgcagcaggg gcacgcggat ctccacaccg aacacgtcgg
    10021 ccttgagctc gagcgtgccc gtcggcttgc ccttgtctcc gaggacctgg accgtaggca
    10081 ttatttcctg tccgccgtgg ccttctgctg ctgggccttc gtggtcttga cgctcttgcg
    10141 caccatcacg aggctgccgc tggcgcccgg caccgcgccc cgcacgagca cgagatgctg
    10201 ctcgggcagc acgcggacca cgctgacgtt cagcaccgtc cgccgctcgc cacccatccg
    10261 gccggggagg gcatggcccg gccagacgcg cgagggatcg gacgaggcgc cgatcgagcc
    10321 gggcgcacga tggaacatcg agccgtgggt cgcgtcaccg ccgtaccagc cgtggcgccg
    10381 gacgccgccc tggaagccct tgcccttggt cacgccgacc acgtccacca gctcccccgg
    10441 cgcgaacatc tcgaccgtca gcgcctggcc cacctggaca ccgccgagct tctcggcagt
    10501 cgccttgtcg agccggagct cgcgcagcac gcgcaccgcg ccgaccccgg ccttcttgaa
    10561 gtgccccgcc atcggcttcg tcacgttctt cctcatcgcc tcgaaaccga gctggagcgc
    10621 gtcgtagccg tggctctcgc gcgtccggat accgacgacc gggcagggac cggcctggat
    10681 gacggtcacg gggatctgac tgccgtcgtc tccgaagacc tgcgtcatgc cgaccttgcg
    10741 tccgatgagg ccctcttttc tcgccatgat cgtcggaggg gctacgcctc ccgtccgata
    10801 cctcccccca gggctctgtc ggagggggcc tcgacggccc tctccgggac ctccccgatc
    10861 gatggcgatg gccacgccgc cgctcgaacc ggagcgtcgt tcacagcttg atctccacat
    10921 ccaccccagc agggagatcg agtttcatca aagagtcgac ggtctggggg gtggggtcga
    10981 cgatgtcgag caggcgcttg tgcgtgcgca tctcgaactg ctcgcgcgag gtcttgtcga
    11041 cgtgcggcga gcggagcacc gtccagcggt tcacgatcgt cggcagcagc ac
//
LOCUS       DATIAZ010000343         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_501524, whole genome shotgun sequence.
ACCESSION   DATIAZ010000343 DATIAZ010000000
VERSION     DATIAZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501524"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /gene="mraW"
                     /locus_tag="VLF19_11805"
     CDS             <1..244
                     /gene="mraW"
                     /locus_tag="VLF19_11805"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HSE93982.1"
                     /translation="PGALRQALPQAAALLEPGARLGVISFHSGEDRIVKLTFRSLTAD
                     GAYAVVEPSPLMPGDDEVRANARARSAKLRVLERAA"
     gene            241..651
                     /locus_tag="VLF19_11810"
     CDS             241..651
                     /locus_tag="VLF19_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93983.1"
                     /translation="MRKQQRLHREHDPRVRRSLAIALTGALLLVVAGLLVVGLRVQQV
                     HLAYRLDALRAERGRAETLLRQLEVEVATLRSPARVAARARQLGLIAPDSRQVRLARE
                     YVTGTTGLAAAERSRVAALGVDETTPVERAPQRP"
     gene            648..>2278
                     /locus_tag="VLF19_11815"
     CDS             648..>2278
                     /locus_tag="VLF19_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSE93984.1"
                     /translation="MSAPGLRTRVLVLAAVLAVAFGGVTARLAWLQIVRHAELTALAE
                     RQYTRTVVLHAPRGPIVDRHGSALAASMPAESLFVQPRYVDDPVRAAARLAPILRMPE
                     AELHAALVTSRPFTWLRRRLSPAAAEQVKALREPGLGLVPEPLRLYPNRELAAHVLGF
                     EGVDGGLEGVERTWESALAGAPGKAVVGRDALGRDLSTQVVLQAPVAGHGVMLTLDAN
                     IQYATERELDAAWRRTGARGAMAVVLDPRTGDLLAAAVRPAFNPNTFLDVPDRDVWRN
                     RIVTDPFEPGSTFKMVLAAAALEEGVARPDDHVFAEYGKITIAKTVIRDWKKFGWLTF
                     AEVLQNSSNVGTIKVALALGTPRYHRYVTAFGFGAPTGVGLPGESRGLLRDPQRWSAL
                     SLPTMSIGQEVSVTALQLAAAFGAVANGGTLMRPRLVRSVFDADGKETRRFEPSAVRR
                     VISAETARTLTRMLTRVVESGTGHNAAIPGYEVAGKTGTAQKPDPTTRRYSRAPGVLS
                     FVGFAPADEPRFVMLVLLDEPKTERWGSEAAAPIFA"
BASE COUNT          272 a          857 c          840 g          309 t
ORIGIN      
        1 gccgggcgcg ctccggcagg cgctgcccca ggcggccgcg ctgctggaac ccggagcgcg
       61 gctcggcgtc atctcctttc actcgggcga agaccgcatc gtgaagctga cgttccgttc
      121 gctcacggcc gacggtgctt acgccgtcgt cgagccgtcg cccctcatgc ccggggacga
      181 cgaggtgcgg gccaacgccc gcgcccggag cgcgaagctg cgcgtgctgg agcgggcggc
      241 gtgagaaagc aacagcgtct ccatcgtgag cacgatcccc gggtccgccg gtcgctcgcc
      301 atcgcgctga ccggcgcact cctcctggtc gtggcgggtc tcctcgtggt cgggctgcgt
      361 gtgcagcagg tgcacctcgc gtaccggctc gacgcgctcc gcgccgagcg aggacgcgcc
      421 gagacgctgc tgcggcagct cgaggtcgag gtggcgaccc tgcgctcgcc cgcgcgcgtg
      481 gccgcgcgcg cgcgccagct cgggctgatc gcgcccgaca gccggcaggt gcggctggcc
      541 cgcgagtacg tgaccggcac caccgggctc gccgccgccg agcggagccg ggtggcggcc
      601 ctcggcgtgg acgagacgac gccggtcgag agggcgccgc agcggccgtg agcgcaccgg
      661 gcctccgcac gcgcgtcctc gtcctcgcgg cagtgctcgc cgtggccttc gggggcgtga
      721 ccgcgcgtct ggcctggctg cagatcgtcc gtcacgccga gctcacggcg ctcgccgagc
      781 gccagtacac ccgcaccgtc gtgctccacg ccccgcgagg cccgatcgtg gaccgtcacg
      841 gatccgccct ggccgcctcg atgccggccg agtcgctctt cgtccagccg cgctacgtgg
      901 acgatcccgt gcgggccgcc gcccgcctgg cccccatcct ccggatgccg gaggccgagc
      961 tgcacgccgc gctggtgacc tcgcggccgt tcacgtggct gcggcgtcgg ctgtcgccgg
     1021 ccgcggccga gcaggtcaag gcgctgcgcg agcccgggct cggcctggtg ccggagccgc
     1081 tgcgcctcta cccgaaccgt gagctcgccg cccacgtgct cggcttcgag ggcgtggacg
     1141 gcgggctgga gggcgtcgag cgcacgtggg agagcgcgct ggcgggcgcg ccgggcaagg
     1201 cggtggtggg gcgcgacgcg ctcgggcgcg acctctcgac ccaggtcgtg ctgcaggcgc
     1261 ccgtggccgg ccacggcgtc atgctcacgc tcgacgccaa catccagtat gcgaccgagc
     1321 gggagctcga cgcggcctgg cgccgcaccg gcgcccgcgg cgcgatggcg gtcgtcctcg
     1381 accccagaac gggcgacctg ctggcggcgg ccgtgcgtcc ggccttcaac ccgaacacct
     1441 tcctcgatgt cccggatcgc gacgtctggc gcaatcggat cgtcacggac ccgttcgagc
     1501 cgggctcgac cttcaagatg gtcctcgccg ccgccgccct cgaggaaggg gtcgcgcggc
     1561 ccgacgacca cgtcttcgcc gagtacggga agatcacgat cgcgaagacg gtcatccgcg
     1621 actggaagaa gttcggctgg ctcacgttcg ccgaggtcct gcagaactcc tcgaacgtcg
     1681 gcacgatcaa ggtggcgctc gccctcggca cgccccgcta ccaccgctac gtgacggcgt
     1741 tcggcttcgg cgcaccgacc ggcgtcggcc tgcccgggga gagccgcggc ctgctgcgcg
     1801 acccgcagcg ctggtcggcg ctctcgctgc cgacgatgtc catcgggcag gaggtgtcgg
     1861 tgaccgcgct ccagctcgcc gcggccttcg gcgcggtggc caacggcggt acgctgatgc
     1921 gtccccggct cgtgcgctcg gtcttcgacg cggacggcaa ggagacgcgg cgcttcgagc
     1981 cgtcggcggt gcgccgggtg atctctgccg agacggcccg cacgctgacg cggatgctca
     2041 cgcgtgtcgt ggagtccggc accggccaca acgcggcgat ccccggctac gaggtggccg
     2101 gcaagacggg taccgctcag aagcccgatc ccacgacgcg gcgctactcg cgcgcgcccg
     2161 gggtgctgtc cttcgtgggc ttcgcgccgg ccgacgagcc gcgcttcgtg atgctcgtcc
     2221 tgctcgacga gccgaagacc gagcgctggg gcagcgaggc ggcggcgccg atcttcgc
//
LOCUS       DATIAZ010000344         9081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1026868, whole genome shotgun sequence.
ACCESSION   DATIAZ010000344 DATIAZ010000000
VERSION     DATIAZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026868"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VLF19_11820"
     CDS             <1..199
                     /locus_tag="VLF19_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE93985.1"
                     /translation="WWAPKLAVDVIHQCLLLHGHYGYTDELPFEQRMRDVIGLEIGDG
                     TAEVMKLIVARELMGREALPY"
     gene            199..945
                     /locus_tag="VLF19_11825"
     CDS             199..945
                     /locus_tag="VLF19_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE93986.1"
                     /translation="MPLKAVTLAERRDLEDEFERFAVAGWPRFLRQRDELGVRRYWPS
                     LFTTFADFQLYVMEGHRVVAVGQTVPFPWDGTPGGLPDSIADVLRLALETRSNGHQPT
                     ALCALAAIADPERRGRGLSAEVLRAMAAVARQHGLATLVAPVRPTLKATYPLAPMERF
                     VRWTRPDGAPLDPWIRTHWRLGAEIVKVAPRTLVIVGRVADWEAWTDMPFPDSGPYVV
                     SGALQPVVIDRQQDRGRYEDPNVWMLHRVA"
     gene            974..1771
                     /locus_tag="VLF19_11830"
     CDS             974..1771
                     /locus_tag="VLF19_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE93987.1"
                     /translation="MDLNLAGKSVIVTGGGSNIGRAIALGFARERANVTIAEIDEGQG
                     KKVAAEAEALGAKAQVVRTDVTKWPDVQAMVKAVEDRFGRVDVLVNNVGWTRDALFVE
                     KTREEWDKEIQLNLWGMINCTRAALDGMIARKGGAIVSLGSDAGRIGEFREGVYAACK
                     AGIIALSKSIAREVGKFGVRLNVVCPGMTMPADDEDIGALSMWNAQENRAWMTDEMRA
                     RIAKVYPLRRVARPDDVVGAVLFLASDTAAGFVTGQTLSVSGGYTMM"
     gene            2132..3778
                     /locus_tag="VLF19_11835"
     CDS             2132..3778
                     /locus_tag="VLF19_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSE93988.1"
                     /translation="MTVIETMLTPERVTAMRAGGWWRDETLDGYLDRWARERADQTAI
                     VDARGRFTWAALARMVERVAHGLQAAGIERGAIVSTQLPNWNEWIVLALACVRVGAIL
                     NPIPPTYRAGELRFILGALETQAFVIPARFRAHDYVAMSDRLRAAAPALRHVWVARGE
                     PGPGMRPFAELTDTAWEARAERRPLAGTDPNAVSEVVFTSGTTGEPKGVMHTSNTVLS
                     ICYPLIDRLGFDSQDVALMCSTFGHQTGFLYGYCLTLLLGSTGVWLDVWNADEAARLV
                     EAERVTYTMGATPFLQDFTYAPAVEGRDIRSLRLFISAGASIPRKLVQDARARLRCAI
                     SAGWGMSENGLVSCSGLADPEEKVFGTDGAPLPGMELRVVDPAGAVLPAGAEGDLLVR
                     GHAQFVGYWKRPEFTREAHTLDGWFRTGDRAVLDADGYVAITGRSKDIIIRGGENISV
                     AEVENLLFAHPKIQSVAVVAMPDPRLVERACAYVIPRRGEAPTLAELAGWLEGHGLAR
                     HKLPERLELVGEFPMTPSGKVQKYRLREDVARKLGLPPVR"
     gene            3786..4598
                     /locus_tag="VLF19_11840"
     CDS             3786..4598
                     /locus_tag="VLF19_11840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSE93989.1"
                     /translation="MTGPEASSDGGPPQGAPRSWPVFATYLLAFVGIVALNLLALALL
                     HEFYPGLPEREITLPALLAGGLASSTALMLALVATVRPLDARRIRLVPGRETGPMLAL
                     IIVGTLALGQALDSATAIAGLANRGAIVEIRRALEGATGADLFAAVLVIGPIAGTAEE
                     IFFRGFMQTALGAVWRPAIAVAVTAAAFALLHVEPIHATQALALGLWFGAVTAGTGSA
                     LPAVAAHVVNNVVFTVLTALSVTIEGIGPNLAVGASSVVAFVGVAIALRHHR"
     gene            4657..5421
                     /locus_tag="VLF19_11845"
     CDS             4657..5421
                     /locus_tag="VLF19_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSE93990.1"
                     /translation="MTTRSLEPFLGARADHRPIAPPSETRRLGVSEAYAIQDRLREAL
                     VARGERVIGWKAGFTSGATQKMFDTDEPVCGFLLASGVFTSGSEVPCSRFAAVVVEAE
                     VAFVMKHDLAGPGVTPPRALQAVEGALPALELVDFRYTGKPVASDLIADGVYANAIVL
                     GTALTRVAHLDLALEGLVYEMNGAIAATNTAAEVLGSPANSLAWIANQLGGRGLGLRA
                     GDLVMSGSVSALLRPRPGDAIRATYTRLGSVAARFV"
     gene            5421..6203
                     /locus_tag="VLF19_11850"
     CDS             5421..6203
                     /locus_tag="VLF19_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE93991.1"
                     /translation="MRLRGRAAVITGAGGGLGQAIALGFAAEGVDLVLGDLGEIAEAT
                     AEGARRAGVKALAVTGDVGRATDMEALTERSVAAFGRLDVLVTCAGIDGPGLLAEQDE
                     ASWTRVIDVNLNGTYRAIRAALPHMIARRHGRIVTISSIFGRHGGYGFITAYTASKHG
                     VIGLTRALAAELGGQGYPDITVNAICPGYVRAGMGVRPQSTRRGPMSGEEIFERYYKR
                     QTPGRRMIEGGEIAHAAVFLALPESGGITGQALNVDGGFIMS"
     gene            6287..7078
                     /locus_tag="VLF19_11855"
     CDS             6287..7078
                     /locus_tag="VLF19_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSE93992.1"
                     /translation="MPTVLYEQKDRVVTITMNRPQAMNAIDPETHYGLVDAWTRFRDD
                     DSAWVAILTGAGDKAFSAGADLKKMVPAAFSGGRGYNPVDHNEYGLGGITRGLEVWKP
                     MIAAVNGFCIAGGLEQALACDFRIAAPHARFGLAEVRWAIMPGAGGTQRLPRAVGLTK
                     ALEMILTAEPISAEEALRVGLINKIVPLPELLPAARALADTLCERGPLALRAAKEAVV
                     RGLSLPMADGLRLEALLSGTLRGTEDAAEGPKAFAEKRQPQFRAR"
     gene            7089..7664
                     /locus_tag="VLF19_11860"
     CDS             7089..7664
                     /locus_tag="VLF19_11860"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HSE93993.1"
                     /translation="MTIDELAAITRARLAERRRRVVDSGHLISAAVLVPIVDRGEPHL
                     VFAKRSERVGHHKGQISFPGGTVCEADRTFLDCALRECEEEIGLPPASVEALGTLDDT
                     ETVATQFVITPFVGRVEPPTAWRPDGGEIEKVIEVPWAALVDRRTFREEIWERNGVRR
                     PVYFYDWQGETIWGATARILKHYIDLVEGQG"
     gene            7681..8463
                     /locus_tag="VLF19_11865"
     CDS             7681..8463
                     /locus_tag="VLF19_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HSE93994.1"
                     /translation="MSWEALLTSVDGGVARITLNRPEARNALSRTLIRELGDALAAFE
                     SDPAARVIVLAGAGDKAFCAGADLKGLGDRGTTLEARESFGGLARILESIPHMKKPVI
                     AQVHGFALAGGCGLAVGCDLSIASDDAVFGLPEITLGMLPLIVMAPILRAAGRKRAML
                     MVLSGERVSAREAFEMGLVSRVVPRADLEREATAVATRMAGFSPTALALAKEAAAVAQ
                     DMEYGKSLHYLRELTTLVSLSDDVKEGITAFFARRKPEWKGR"
BASE COUNT         1231 a         3137 c         3383 g         1330 t
ORIGIN      
        1 gtggtgggcg cccaagctgg cggtggacgt catccaccag tgcctgctgc tgcacgggca
       61 ctacggctac accgacgagc tccccttcga gcagcgcatg cgcgacgtca tcgggctcga
      121 gatcggcgac ggcaccgccg aagtgatgaa gctcatcgtc gcccgcgagc tgatgggacg
      181 cgaagcgctg ccatactgat gccgctcaag gccgtcaccc tcgccgagcg ccgtgacctc
      241 gaggacgagt tcgagcgctt cgccgtcgcc ggctggcccc gcttcctgcg gcagcgcgac
      301 gagctcgggg tgcgccgtta ttggccctcg ctcttcacga ccttcgccga cttccagctc
      361 tacgtgatgg aggggcaccg ggtcgtcgcc gtcggtcaga cggtgccgtt cccgtgggac
      421 ggcacccccg gcgggttgcc ggactcgatc gcggacgtgc tccgcctcgc gctcgagacg
      481 cgcagcaacg gacaccagcc gacggcgctg tgcgcgctgg cggcgatcgc cgacccggag
      541 cggcggggcc ggggcctgag cgccgaggtg ctgagagcga tggccgccgt cgcgcggcag
      601 cacgggctgg ccacgctggt ggcgccggtg cggcccacgc tcaaggccac gtacccgctg
      661 gcgcccatgg agcgcttcgt gcgctggacg cggcccgacg gcgcgcccct cgatccgtgg
      721 atccggacgc actggcgtct gggggccgag atcgtgaagg tggctccacg aacgctcgtg
      781 atcgtcggtc gcgtcgcgga ctgggaggcc tggacagaca tgccgttccc cgacagcggg
      841 ccgtacgtgg tgtccggggc gctccagccc gtcgtgatcg accggcagca ggatcgagga
      901 cgatacgagg accccaacgt ctggatgctc caccgggtcg cctgacaccg accccggacg
      961 cggaggcgca acgatggatc tgaacctggc gggcaagagc gtcatcgtca ccggcggcgg
     1021 ctcgaacatc gggcgggcga tcgcgctggg gttcgcgcgc gagcgggcga acgtcaccat
     1081 cgccgagatc gacgagggcc agggcaagaa ggtcgccgcc gaggccgagg cgctcggggc
     1141 gaaggcgcag gtggtccgca ccgacgtcac gaaatggcct gacgtgcagg ccatggtgaa
     1201 ggccgtggag gatcgattcg gccgggtgga cgtgctcgtc aacaacgtcg gctggacgcg
     1261 cgacgcgctc ttcgtcgaga agacccggga ggagtgggac aaggagatcc agctcaacct
     1321 ctgggggatg atcaactgca cccgcgccgc gctcgacggc atgatcgcgc gcaagggcgg
     1381 cgcgatcgtc agcctcggct ccgacgccgg gcggatcggc gagtttcgcg agggcgtcta
     1441 cgcggcctgc aaggccggga tcatcgccct cagcaagtcc atcgcccgcg aggtcgggaa
     1501 gttcggtgtt cgcctgaacg tggtgtgccc cgggatgacg atgcccgccg acgacgagga
     1561 catcggggcg ctcagcatgt ggaacgctca ggagaaccgc gcgtggatga ccgacgagat
     1621 gcgcgcgcgg atcgccaagg tctacccgct gcgccgggtg gcgcgccccg acgacgtcgt
     1681 cggcgccgtg ctctttctcg cctccgacac cgcggccggc ttcgtcaccg gccagacgct
     1741 gtccgtcagc ggcggctaca cgatgatgta gcgaggttgg cggccgaggc gtcggccggg
     1801 gtcgggggca gccggggcac agggggtccg ccgacccctg atgcgtggcg cgatgtatgc
     1861 ggttgcgtag gtccatcgaa gctgtcgaac gtgtgacgga ttgcggggac caggtcggcg
     1921 gaccccctgt gccccgtctg cccccgaccc cggcccggcc gccggtcggc gagctttccg
     1981 tttcgggaca ccgggcgcgg ccagtgcgcc ctctgggcgc ggctcctgtt ggcgcgggcg
     2041 cgtgctgcta gccatcgctg ttgttggtcg gtgcgtgtcg gtggcgggcg cgttgacttc
     2101 aggcgagtgg gcctcgtacc ctgcctccgc gatgactgtt atcgagacca tgctgacgcc
     2161 ggagcgggtg acggccatgc gggcgggcgg gtggtggcga gacgagacgc tcgacgggta
     2221 tctcgaccgg tgggcgcgcg agcgggcgga ccagacggcg atcgtggacg cgcgcggacg
     2281 attcacctgg gcggcgctgg cccggatggt cgagcgcgtg gcgcacgggc tgcaggcggc
     2341 ggggatcgag cggggcgcca tcgtctccac gcagctgccg aactggaacg agtggatcgt
     2401 gctggccctg gcctgcgtcc gggtgggcgc gatcctcaat cccatccccc cgacctatcg
     2461 cgccggcgag ctacgcttca tcctcggcgc tctcgagacg caggccttcg tgatcccggc
     2521 gcgcttccgg gcccacgact acgtggcgat gagcgaccgg ctgcgcgctg cagccccggc
     2581 gctgcggcac gtgtgggtgg cccggggcga gccggggccg ggcatgcggc cattcgccga
     2641 attgaccgac acggcctggg aggcccgcgc ggagcgccgc ccgctggccg gcaccgatcc
     2701 caacgcggtc tccgaggtcg tcttcacgtc gggcacgacg ggcgagccga agggcgtcat
     2761 gcacacgtcc aacaccgtgc tatcgatctg ctacccgctg atcgaccggc tcggcttcga
     2821 ttcccaggac gtggcgctca tgtgctcgac cttcggccac cagaccggct tcctctacgg
     2881 ctactgcctc acgctcctgc tcggctccac cggcgtctgg ctcgacgtct ggaacgccga
     2941 cgaggcggcc cggctcgtcg aggccgagcg tgtgacctac acgatggggg cgacgccgtt
     3001 cctccaggac ttcacctacg cgccggcggt ggaggggcgc gacatccggt cgctccggct
     3061 cttcatctcc gcgggcgcgt cgatcccccg caagctcgtc caggacgcgc gagcgcggct
     3121 ccggtgcgcg atctccgcgg ggtggggaat gtccgagaac ggcctcgtct cgtgcagcgg
     3181 cctcgccgac cccgaagaga aggttttcgg gaccgacggc gcgccgctgc cgggcatgga
     3241 gctgcgcgtg gtggacccgg ccggcgccgt cttgccggcc ggcgccgagg gcgacctgct
     3301 cgtccgcggt cacgcgcagt tcgtcggata ctggaagcgc ccggagttca cccgcgaggc
     3361 gcataccctc gacgggtggt tcaggaccgg cgatcgcgcg gtgctggacg ccgacggcta
     3421 cgtggccatc accgggcgct ccaaggacat catcatccgt ggcggcgaga acatctcggt
     3481 ggccgaggtc gagaatcttc tcttcgcgca tcccaagatc cagtcggtcg ccgtggtcgc
     3541 catgcccgac ccgcgtctcg tcgagcgggc gtgcgcgtac gtgatcccgc gccgcggcga
     3601 ggcgcccacg ctggcggagc tggccgggtg gctcgagggc cacggcctcg ccaggcacaa
     3661 gctgccggag cgtctcgagc tggtcggcga gttccccatg accccgagcg ggaaggtcca
     3721 gaagtaccgg ctgcgcgagg acgtggcgcg caagctcggc ctgccgcccg tgcgttgagg
     3781 tgcgagtgac gggaccagag gcctcgtcgg acggcgggcc gccgcaggga gcgccgcgct
     3841 cctggccggt gttcgccacg tacctgctgg ccttcgtggg catcgtcgcc ctgaacctgc
     3901 tggcgctggc cctccttcac gagttctacc ccgggctacc cgagcgcgag atcaccttgc
     3961 cggcgctgct cgccggcggt ctcgcctcgt cgaccgcgct catgctggcc ctcgtggcca
     4021 ccgtccgacc gctcgacgcc cggcgcatac ggctcgtgcc cgggcgcgag acggggccga
     4081 tgctcgccct gatcatcgtg ggcacgctgg ccctggggca ggcgctggac tccgcgacgg
     4141 cgatcgcggg tctggccaac cggggcgcga tcgtggagat ccgccgcgcg ctggagggcg
     4201 cgaccggcgc cgacctcttc gcggcggtgc tcgtcatcgg cccgatcgcg gggaccgcgg
     4261 aggagatctt cttccggggc ttcatgcaga ccgcgctcgg cgccgtctgg cggccggcga
     4321 ttgcggtggc cgtgaccgcg gcggccttcg cgctgctgca cgtggagccg atccacgcga
     4381 cgcaggcgct cgcgctgggg ctctggttcg gcgccgtcac cgcggggacg ggtagcgcgc
     4441 tgcccgccgt cgccgcgcac gtcgtcaaca acgtggtctt cacggtcctc accgcgctga
     4501 gcgtcaccat cgagggtatc gggccgaacc tcgccgtcgg ggcaagctcg gtggtcgcgt
     4561 tcgtgggcgt cgccatcgcg cttcgtcacc atcgctgact tgcgacgtgg cggccgacag
     4621 cggtaggctt gaagcccgca tgacggccga ctcccgatga cgacccggtc gctcgagcca
     4681 tttctcggtg cgcgcgcgga tcaccgtccg atcgcgcctc cgagcgagac acggcgcctc
     4741 ggcgtgtcgg aggcctacgc gatccaggac cgtctccgcg aggccctggt tgcgcgcggc
     4801 gagcgcgtga tcggctggaa ggcggggttc accagcgggg ccacgcagaa gatgttcgac
     4861 accgacgagc cggtctgtgg cttcctgctg gccagcggcg tcttcaccag cgggtccgag
     4921 gtgccgtgct cgcgcttcgc ggccgtcgtg gtcgaggccg aggtggcctt cgtgatgaag
     4981 cacgatctgg ccggtcccgg cgtcacgccg ccgcgcgcgc tccaggccgt ggagggcgcg
     5041 cttccggcgc tcgagctggt cgactttcgc tacacgggca agcccgtcgc cagtgacctg
     5101 atcgccgacg gcgtgtacgc gaacgccatc gtgctgggga cggcgctgac gcgggtggcg
     5161 cacctcgacc tcgcgctgga ggggctcgtc tacgagatga acggcgccat cgcggcgacc
     5221 aacacggcgg cggaggtgct gggcagcccg gcgaactcgc tggcatggat cgccaaccag
     5281 ctcggcgggc gcgggctcgg gctgcgcgct ggcgatctgg tgatgtcggg ctcggtgtcg
     5341 gcgctgctgc ggcccaggcc cggcgacgcc atccgcgcga cgtacacgcg gctcggctcg
     5401 gtcgccgccc gcttcgtctg atgcggctac gtggccgcgc ggcggtcatc accggtgcgg
     5461 gagggggcct cgggcaggcg atcgcgctcg ggttcgccgc cgagggggtg gacctggtgc
     5521 tcggcgacct cggcgagatc gccgaggcga ccgccgaagg agcacgccgc gcgggagtga
     5581 aggccctggc ggtgaccggg gacgtcgggc gcgccaccga catggaggcg ctcaccgagc
     5641 gctctgtcgc ggcgttcggc cgcctggacg tccttgtgac ctgcgccggc atcgacggcc
     5701 cgggcctgct cgccgagcag gacgaggcgt cctggactcg cgtcatcgac gtcaacctga
     5761 acggcaccta ccgggcgatc cgggccgcgc tgccgcacat gatcgcccgg cgtcacggcc
     5821 gcatcgtgac gatctcgtcg atcttcggac gccacggcgg ctacgggttc atcaccgcct
     5881 acacggcctc caagcacggc gtgatcggcc tgacgcgcgc gttggccgcc gagctgggcg
     5941 gccagggcta cccggacatc accgtcaacg cgatctgccc gggctacgtg cgggccggca
     6001 tgggcgtgcg cccgcagtcc accaggcgcg ggccgatgtc gggcgaggag atcttcgagc
     6061 gctactacaa gcgccagacg cccggccggc gcatgatcga gggcggggag atcgcccacg
     6121 ccgccgtctt cctggcgctg ccggagtccg gcgggatcac cggccaggcg ctcaacgtcg
     6181 acggcggctt tatcatgtcc tgaggccggc gattgccccg gtgcccgcgc agcgacgacg
     6241 cctgggcgcc catcgtgaac gccggggagg gacgaggagg cggcggatgc cgaccgtgct
     6301 gtacgaacag aaggaccggg tcgtgacgat caccatgaac cggccccagg cgatgaacgc
     6361 gatcgacccg gagacgcact acgggctcgt ggacgcctgg acgcgcttcc gtgacgacga
     6421 ctccgcctgg gtggcgatcc tcaccggcgc gggcgacaag gccttctcgg cgggcgccga
     6481 tctgaagaag atggtcccgg cggcgttctc cggcggccgt ggctacaacc cggtggatca
     6541 caacgaatat gggctaggcg gcatcacgcg cgggctcgag gtgtggaagc cgatgatcgc
     6601 cgccgtcaat ggcttctgca tcgcgggggg tctcgagcaa gcgctggcct gcgacttccg
     6661 catcgcggcg ccgcacgcga ggttcggcct ggccgaggtg cgctgggcca tcatgcccgg
     6721 tgccggaggc acgcagcggc tgccgcgcgc ggtcgggctc accaaggcgc tcgagatgat
     6781 cctgaccgcc gagccgatct ccgccgagga ggcgctgcgc gtcgggctca tcaacaagat
     6841 cgtgccgctg ccggagctgc tgcccgccgc gcgggcgctg gccgacacgc tgtgcgagcg
     6901 ggggccgctc gcgctgcggg cggcgaagga ggcggtggtg cgcggcctct cgctgccgat
     6961 ggccgacggg ctccggctcg aggccctgct gagcggcacg ctccgtggca ccgaggacgc
     7021 cgccgagggg ccgaaggcat tcgccgagaa gcgccagccg cagttcaggg cgcgctgagc
     7081 gccgtgcgat gaccatcgac gagctggccg cgatcacacg ggcccggctc gccgagcgcc
     7141 ggcgccgcgt ggtcgattcc gggcacctga tctccgccgc ggtgctcgtc cccatcgtcg
     7201 atcgtggcga gccgcatctg gtgttcgcga agcgcagcga gcgggtaggc caccacaagg
     7261 ggcagatctc gttcccgggt ggcacggtct gcgaggcgga ccggacgttc ctcgactgcg
     7321 cgcttcgcga gtgcgaggag gagatcggcc tgccgccggc gtccgtggag gcgctcggca
     7381 cgctggacga caccgagacc gtcgccactc agttcgtgat cacgcccttc gtcggccgcg
     7441 tcgagccgcc gacggcctgg cggccggacg gcggggagat cgagaaggtg atcgaggtgc
     7501 cgtgggcggc gctcgtcgac cgtcggacgt tccgcgagga gatctgggag cgcaacggcg
     7561 tgcggcggcc cgtgtacttc tacgactggc agggcgagac catctggggc gctaccgccc
     7621 gcatcctcaa gcactacatc gatctggtcg aagggcaggg gtgacgatcg gcggtcgcgc
     7681 atgagctggg aggcgctgtt gacgtcggtc gacggcggcg tggcccggat cacgctgaac
     7741 cgcccggagg cccgcaacgc gctctcgcgc acactcattc gcgagctggg ggacgcgctg
     7801 gccgcgttcg agtcggatcc ggccgcgagg gtgatcgtgc tggccggcgc cggggacaag
     7861 gcgttctgcg ccggcgccga cctcaagggc ctcggcgacc ggggtacgac gctcgaggcg
     7921 cgcgagtcct tcggcggcct ggcccgcatc ctcgagtcga tcccgcacat gaagaagccg
     7981 gtgatcgccc aggtgcacgg gttcgcgctg gcgggcggct gcgggctcgc cgtcggctgc
     8041 gacctgtcga tcgcctccga cgacgccgtg ttcggcctgc cggagatcac gctcggcatg
     8101 ctgccgctca tcgtcatggc gccgatcctc cgcgccgccg gccgcaagcg ggcgatgctc
     8161 atggtgctct cgggcgagcg cgtgtcggcg cgcgaggcct tcgagatggg gctggtgagc
     8221 cgcgttgtgc cgcgcgccga cctcgagcgc gaggcgacgg cggtggcgac acgcatggcg
     8281 gggttcagcc cgacggcact cgcgctggcc aaggaggcgg cggccgtcgc gcaggacatg
     8341 gagtacggca agtcgctcca ctacctgcgc gagctgacaa cgctcgtctc cctgtccgac
     8401 gacgtcaagg agggcatcac ggcgttcttc gccaggcgga agccggagtg gaaggggcgc
     8461 tgaccgggtg cggcaaagcc ctgaggaacg ctggtggggt atggccggcg gcacgccccg
     8521 gccggtgctc agaccggcgg ccggtttcct cagggccggc ctcacggtag tcccggccgc
     8581 cgggtgatgt caagcgaccg cacggcgtcg accgagcgcg gggacggcgg cgatcgcgtc
     8641 ggcctctgcg cccgctgcct tcacgccgac ccggtcacgt ccgcgcgcgg ctcgaccttc
     8701 tggcgctgcc gcgtgagcga ccacgacccc cgctttgcga agtatccgcg cctcccggtg
     8761 gtcgcatgtt ccgccttcct tgccaggacc gcgtcggact gagctacccg gcaacggtgt
     8821 cttgcctggc catccggact cgttgctgag cttgaccaaa cccgcccggg acggccttgt
     8881 ccacctcaca ctcgaggatc gatactaacc ctttcctcct gagacggggc tgaggaacgg
     8941 agaactcggg caaccagctg tgctccggca gggagcagca cgtgccaccg ggggagagcc
     9001 cgcggtgcaa ggagggctca tgaactacgg agtcgtgctt ccgatctggc agctcacggt
     9061 gtccgaagcg gaggggctga c
//
LOCUS       DATIAZ010000345         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1291311, whole genome shotgun sequence.
ACCESSION   DATIAZ010000345 DATIAZ010000000
VERSION     DATIAZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291311"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VLF19_11870"
     CDS             complement(<1..240)
                     /locus_tag="VLF19_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE93995.1"
                     /translation="MPAGTWVGRFLVVAAAVLPLALFLASERRIAGAAGFPLDDSWIH
                     LHFARNLAEGAGFSYNPGVPVAGSTAPLWTVLLGGA"
     gene            complement(436..2037)
                     /locus_tag="VLF19_11875"
     CDS             complement(436..2037)
                     /locus_tag="VLF19_11875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSE93996.1"
                     /translation="MTPSAPRLDFACASALSILTVLSRLPYRARMLYNWDAVQFALAL
                     REYDIGKHQPHPPGYILYVALGRLVNAGLDDPTGAYVLLAVAFSGATTFVVYHLALAL
                     YDRATALAAAALLAVSPLFWFYGSVGLTYAGEALCATTIAYFAFRALRGSETDAWLCA
                     GYLGLAGGLRQSILPLLFPLWLVSTVVGVRRARTIAIGLAIMSVAVLMWLVPMLWLTG
                     GLERYVAASLELADSIVRSTSIVGGPFETTLRVSRYVLESVLVGLGPLALAVALLPWY
                     VRRHGWGRREWFLTCWTVPPVVVYVLVHFGQAGYVLTFLPALVVLLSRVLVAALGAAL
                     AAGSRARAVATASVVVGVVLVNGSFFVSARPAPRDFDAPRRAWVRTAQDEAFDWIWSR
                     TAAALREHEQVVQTFVAAIRGLYRPEDTLVVTELGNPRSYPWLRHAMYYLPEYAIYEL
                     RVGELPPGYYAPRLAVSMTRVPDAAVPVPAAVKRIVWFVDHWSPFSERPPGLQEMELP
                     YGRYLYVLPFGRRPVTYAGYTFLRE"
     gene            complement(2073..2264)
                     /gene="tatA"
                     /locus_tag="VLF19_11880"
     CDS             complement(2073..2264)
                     /gene="tatA"
                     /locus_tag="VLF19_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSE93997.1"
                     /translation="MFGLGYQELLIILVIVLILFGANRLPELARSLGSSVKEFKKGVN
                     EVKAEDTTVAAKKEDEKKA"
     gene            complement(2303..>3664)
                     /locus_tag="VLF19_11885"
     CDS             complement(2303..>3664)
                     /locus_tag="VLF19_11885"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HSE93998.1"
                     /translation="RLPIDRVFTVKGFGTVVTGTLMAGRLAADDRVEVYPRGLTAKVR
                     GLQTHGAPVVEALAGQRTAVNLQSLERASVERGDVVGLAGTLVSSVLVDGTLELLADA
                     PRPLKSRDRVRFHTGTSEIMARALLLDRAALEPGQRAFARFRLESPLVAVPGDRFVIR
                     SYSPIVTIGGGTLLDVDPPRFKRKAPALIAHLALLERGTPEEVVEEHVRHVGVGGARV
                     ATLAGRVPFGPDGLRALLVALEKAGRVLAVERDWYLHPESVARLRGLVVAALEQFHRA
                     NPLRSGMSREELRGRAGAPDERVFAHMLGILAAEGAVRVERDKASLGDHTVRLSAEQQ
                     RVVDRVEREFLEAAAAPPSAEEALARAGVRGGEDHELFQYLLEAKRLVRVKEALYFHP
                     SALATIQDKVVAYLRERKEIGPGDIKDLLGISRKYAIPLLEHLDARRVTVRQGERRVL
                     RGG"
BASE COUNT          604 a         1315 c         1313 g          432 t
ORIGIN      
        1 ggcgccgccg agaaggaccg tccagagcgg cgcggtcgag ccggcgacgg ggacgcccgg
       61 gttgtacgag aagccggcgc cctcggcgag attgcgcgcg aagtgcaggt ggatccagga
      121 gtcgtcgagc gggaagccgg ccgcgccggc gatgcgccgc tcgctcgcga ggaacagcgc
      181 gagcggcaag acggccgccg cgacgaccag gaacctgccg acccacgtcc cggctggcac
      241 tagtgtcccg cctgcgggct cacatcaacg atcccgcgcg gcgaggccga cagacgcacg
      301 gtagggatcg agcgacccgt tcgcgccgcg ccggaaggga aacgcaggtg acacgacggc
      361 ccccatgtaa ccccgtcgcc tgacgctcgg tccgcccggc ctcaccggga gacgactcga
      421 tatcacggca cggttctact cgcggaggaa ggtgtacccg gcgtaggtca cggggcgccg
      481 cccgaacggc agcacgtaga ggtaacggcc gtacggcagc tccatctcct ggagcccggg
      541 cggacgctcc gagaacgggc tccagtggtc gacgaaccac acgatccgct tcaccgccgc
      601 gggcacgggg accgcggcgt ccgggacacg cgtcatggac accgcgaggc gtggggcgta
      661 gtagccgggg ggcagctcgc cgacgcgtag ctcgtagatc gcgtactcgg gcaggtagta
      721 catcgcgtgg cgcagccagg ggtacgagcg cgggttgcca agctcggtga cgacgagcgt
      781 atcctcggga cggtagagtc cgcggatcgc cgccacgaac gtctggacca cctgctcgtg
      841 ctcgcgcagc gcggcggcgg tgcggctcca gatccagtcg aacgcttcgt cctgcgcggt
      901 cctgacccag gcccgccgtg gtgcgtcgaa gtcgcgcggc gccgggcggg cgctgacgaa
      961 gaacgacccg ttgacgagca cgacgccgac gacgacggag gccgtcgcca cggcccgcgc
     1021 gcgggagccc gcggcgaggg cggcgccgag cgccgccacc agcacgcgcg agagcagcac
     1081 gacgagcgcc ggcaggaagg tcagcacgta gccggcctga ccgaaatgca cgaggacgta
     1141 gacgaccacc ggggggaccg tccagcacgt caggaaccac tcccgccggc cccagccgtg
     1201 ccgacgcacg taccagggta gcagcgccac cgccagcgcg agcggcccga gacccaccag
     1261 gacggactcc agcacgtatc tcgacacccg cagggtcgtc tcgaacggcc cgcccacgat
     1321 cgacgtcgac ctgacgatgc tgtcggcgag ctcgagcgag gcggccacgt aacgctcgag
     1381 accgccggtc agccacagca tcggcacgag ccacatgagc accgcgacgc tcatgatcgc
     1441 gagcccgatc gcgatcgtcc gggcgcgccg gacgccgacc accgtcgaga cgagccacag
     1501 cgggaacagc agcggcagga tcgactgccg cagtcccccg gccaggccca ggtagccggc
     1561 gcagagccac gcgtcggtct cgctgccgcg cagcgcgcgg aaggcgaagt acgcgatggt
     1621 ggtggcgcac agcgcctccc ccgcgtaggt caggccgacc gatccgtaga accagaacag
     1681 cgggctcacc gcgagcagcg ccgccgcggc cagcgccgtc gcccggtcgt agagcgccag
     1741 cgccaggtgg tagacgacga aggtggtggc gccgctgaag gccacggcca gtagcacgta
     1801 ggcgcccgtg ggatcgtcga gcccggcgtt cacgagccgg cccagcgcca cgtagaggat
     1861 gtagcccggc gggtggggct ggtgcttgcc gatgtcgtac tcgcgtagcg cgagcgcgaa
     1921 ctgcacggcg tcccagttgt agagcatccg cgcccggtat ggcaggcggg agagcacggt
     1981 gaggatcgac agggcgctcg cgcacgcgaa gtccagccgc ggcgcggaag gggtcatgac
     2041 gggcaggagt ctaggggagg ggaggaagag cgctaggcct tcttctcgtc ttccttcttc
     2101 gcggccaccg tcgtgtcctc cgccttcacc tcgttgacgc ccttcttgaa ctccttgacg
     2161 ctcgaaccca gcgatcgagc caactccggt agccgattcg cgccgaagag gatcagcacg
     2221 atcaccagga tgatcagcaa ctcttggtag ccgagaccaa acatacgccg ctcctttccg
     2281 ccgctccaac catactccgc gcctacccgc cccgcaagac ccgacgctcg ccctgacgca
     2341 cggtgacccg gcgcgcgtcg aggtgctcga ggagcgggat cgcgtacttg cgggagatcc
     2401 ccagcaggtc cttgatgtca ccggggccga tctccttccg ctcgcgcagg tacgcgacga
     2461 ccttgtcctg gatggtggcg agggcggagg ggtggaagta gagcgcctcc ttgacccgca
     2521 cgagccgctt ggcctcgagc aggtactgga acagctcgtg gtcctcgccg cccctgacgc
     2581 cggcgcgcgc cagtgcttcc tcggcgctcg ggggagccgc cgcggcctcg aggaactcgc
     2641 gctcgacgcg gtccacgacc cgctgctgct ccgccgagag gcgcaccgtg tggtcaccga
     2701 ggctcgcctt gtcgcgctct acccgcacgg cgccctccgc ggcgaggatg ccgagcatgt
     2761 gtgcgaacac gcgctcatcg ggcgcgccgg cacggccacg cagctcttcg cgcgacatgc
     2821 ccgaccgcag agggttggcg cggtggaact gctcgagcgc ggccacgacg agccctcgca
     2881 gtcgcgcgac gctctcggga tgcaggtacc agtcgcgctc gacggccagg acgcggcccg
     2941 ccttctcgag cgccacgagc agcgcgcgga gcccgtccgg accgaagggg acgcggccgg
     3001 cgagcgtggc cacgcgcgcg ccgcccaccc cgacgtggcg cacgtgctcc tcgaccacct
     3061 cctcgggcgt gccgcgctcg agcagagcga ggtgggcgat gagggcgggc gccttgcgct
     3121 tgaagcgcgg cgggtccacg tcgagcagcg tgccgccgcc gatggtgacg atcggcgagt
     3181 acgagcggat cacgaaccgg tcgccgggca cggccaccag cggcgactcg agccggaagc
     3241 gcgcgaacgc ccgctggccg ggctcgagcg ccgcgcggtc cagcaagagc gcacgcgcca
     3301 tgatctcgct ggtcccggtg tggaagcgca cgcggtcccg cgacttcagt ggccgcgggg
     3361 cgtcggccag cagctccagc gtgccgtcca cgagcaccga ggacacgagc gtgccggcca
     3421 gccccacgac gtcgccgcgc tcgacggagg cccgctccag agactgaaga ttcaccgccg
     3481 tgcgctggcc ggcgagcgcc tcgacgaccg gcgcgccgtg ggtctgcagc ccgcgcacct
     3541 tcgcggtgag ccccctgggg tagacctcga cacgatcgtc ggccgccagg cgtcccgcca
     3601 tgagcgtgcc ggtgaccacg gtgccgaagc ccttgacggt gaacacgcgg tcgatcggca
     3661 ggcg
//
LOCUS       DATIAZ010000346         4547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_503914, whole genome shotgun sequence.
ACCESSION   DATIAZ010000346 DATIAZ010000000
VERSION     DATIAZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503914"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VLF19_11890"
     CDS             complement(<1..1142)
                     /locus_tag="VLF19_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014611416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HSE93999.1"
                     /translation="MDQILIGKGERPVYLLARYGNRHGLVAGATGTGKTISLMVMAEG
                     FSRLGVPVFMADVKGDVAGLAQAGVVSEKLRERAALVGLEGYTPEASPVVFWDLYGKT
                     GHPVRTTVSEMGPSLLARILKVNDTQADVLEVAFRLADDRGLLLLDLDDLRALLGFAA
                     DNRKEISTQYGLVSAQSVGAIQRSLLALEREGGEALFGEPALDLADLMRTDLSGRGVV
                     SILAADQLILKPRLYSSFLLWLLSRLFEDLPEVGDLDKPKLVFIFDEAHLLFEDAPPA
                     LRERVEQVVRLIRSKGVGVYFCSQLPDDVPNEILGQLGNRIQHALRAYTPRDQKAVRT
                     AAETFVPNPKLDVARVISELGVGEALVSTLQDKGVPMPVERALMCPP"
     gene            1466..3139
                     /gene="treS"
                     /locus_tag="VLF19_11895"
     CDS             1466..3139
                     /gene="treS"
                     /locus_tag="VLF19_11895"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HSE94000.1"
                     /translation="MPNDWYKDAVFYEAHVKAFQDGDGDGVGDFAGLTARLDYLKDLG
                     VDCLWILPMYPSPLRDDGYDIADFYGIHPSYGTVEDFQKFLDAAHARGLRVIADLVMN
                     HTSDAHPWFQASRSDPAGPHGDWYVWSDTDRKYRDARVIFIDTEKSNWTLDAARRQYF
                     WHRFFSHQPDLNYDSAAVRRAMLDVMRFWLDRGLDGFRCDAVPYLLEREGTNCENLPE
                     THGILKEFRRVIDQEYGGDRVLLAEANQWPEDVRPYFGEGDEFHMAFHFPLMPRLYMA
                     IRREERLPILDIFTHTPAIPSRCQWCLFLRNHDELTLEMVTSEERDYMYYAYAQDPQA
                     KLNLGIRRRLAPLMDNDRRRIELLNCLLLTLPGSPIVYYGDEIGMGDNIYLGDRNGVR
                     TPMQWSRDRNAGFSSADESRLYLPVIGDPVYGYQAVNVEAQSKTPTSLLNTMRRLIAA
                     RKGCAAFGRGSIEFLRPLNQSVLAFVRAHGDETILVVANLSGRSQPVSLDLARHRGAV
                     PVELLGQTRFPPLSEHPYFLSLGPHGFYWFRLERADRQPPRYGIEDAAL"
     gene            3114..4451
                     /locus_tag="VLF19_11900"
     CDS             3114..4451
                     /locus_tag="VLF19_11900"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94001.1"
                     /translation="MASRTPPSDALAAWIAGRRWFATKTRRISAVVIEDAVPLGGGVL
                     ALARVTLDDGTEDRYALPLSPAAAGQVADALDDPAFCQALLDLVGRGGAAEGGRGELR
                     GVPTAAFPSPPPGDTTPRRLGGEQSNTSVAFGSTLILKHFRRLSPGLSPEEEIGRFLT
                     ERVRFPHVPALCGHLEYRRRDGGHFTLGIVQTLIAGARDGWEWTLDALGDPTRRRAVI
                     EMLARLGAATAALHGALASAADDPDFAPEPIDDADAGAWAGAVRAQLAAAAEALSEAR
                     LAVDLPVDEGLRGLVGATKIRHHGDFHLGQTLYREASGEPFIIDFEGEPLRPLAERRR
                     KHTPLRDVAGMLRSIDYAAAAARPDGDGWADGWRTEAARAFTDAYRAGARGAPFVPAS
                     DEAFAHAVAVLVVEKAAYEIVYEANNRPEWVAIPRRGLLSAASALRARGAGAA"
BASE COUNT          669 a         1677 c         1544 g          657 t
ORIGIN      
        1 ggcggacaca tcagcgcgcg ctccaccggc atcggcacgc ctttgtcctg gagcgtggag
       61 accagcgcct cgccgacacc cagctcggaa atgacgcggg cgacgtcgag cttggggttg
      121 gggacgaagg tttcggccgc ggtgcgcacg gccttctggt cacgcggggt gtacgcgcgc
      181 agcgcgtgct ggatgcggtt gccgagctgg cccaggatct cgttgggcac gtcgtcagga
      241 agctgcgaac aaaagtacac tcccacgccc ttggaccgga tgagccgcac gacctgctcc
      301 accctctcgc gcagcgccgg gggcgcgtcc tcgaacagca ggtgggcctc gtcgaagatg
      361 aacaccagct tcggcttgtc gaggtccccc acctccggca ggtcctcgaa cagcctcgac
      421 aggagccaca gcaggaagct cgagtagagg cgcggcttaa ggatgagctg gtcggctgcg
      481 aggatgctca cgacgccgcg gccactgagg tcggtgcgca tcaggtccgc gaggtccagc
      541 gcgggctccc cgaacagggc ttcgccgcct tcccgctcca gcgccagcag ggagcgctgg
      601 atggcgccca ccgactgcgc gctgaccagc ccgtactgcg tcgagatctc cttgcggttg
      661 tcggcggcga acccgagcag cgcgcgcaga tcgtcgaggt ccagcagcag caggccccga
      721 tcgtccgcca gcctgaaggc gacctcgagg acgtccgcct gggtgtcgtt gaccttgagg
      781 atccgagcga gcaagctcgg gcccatctcg ctgacagtgg tgcgcaccgg gtggccggtc
      841 ttgccgtaga gatcccagaa caccacggga ctggcctcgg gcgtgtagcc ttcgaggccg
      901 accagggcgg cgcgctcccg gagcttctcg ctgaccaccc cggcctgcgc cagcccggcg
      961 acgtcaccct tcacgtcggc catgaacacc ggcacgccca ggcgcgagaa gccctccgcc
     1021 atcaccatca gcgagatggt cttgccggtg ccggtggcgc cggcgaccag accgtggcgg
     1081 ttgccgtacc gggcgagcag atagaccggc ctctccccct tgccgatcag gatctggtcc
     1141 actcgcgcct cctcgactcg cggcgatggc ctcgacatgc tcgtctcgat ctcgaggctg
     1201 cgagccatgt cgctcgagca cctgactcga accgcatccc ctcgctgcca cggcgacgca
     1261 gcgcgctcgg tcggccgagc gcggtgcctg gccgagcgcc aggcgctctg gcacgcttcg
     1321 gcagggcggc ctcgcgcggt gcgccattgt ccccgtccgc cggacggtga ccattccggg
     1381 tgagaatagt ccgcccgcca agcacggaca agagtcaccg gaccgaccgc cggcgagccg
     1441 gcggtagaat cgagacagag ccccgatgcc caacgactgg tacaaggacg cggtcttcta
     1501 cgaggcgcac gtcaaggcgt tccaggacgg tgacggcgac ggcgtcggcg acttcgcggg
     1561 gctgacggcc cggctcgatt acctgaagga cctcggcgtg gactgcctgt ggatcctgcc
     1621 gatgtatccg tcgcccctgc gcgacgacgg ctatgacatc gccgatttct acgggatcca
     1681 cccctcctac ggcaccgtcg aggacttcca gaagttcctc gacgccgctc acgcccgtgg
     1741 actgcgcgtc atcgccgatc tcgtgatgaa ccacacctcc gacgcccatc cctggttcca
     1801 ggcctcgcgc tcggacccgg cgggcccgca tggcgactgg tacgtgtggt cggacaccga
     1861 ccggaagtac cgcgacgcgc gcgtcatctt catcgacacc gagaagtcca actggacgct
     1921 cgacgccgcg cgccggcagt acttctggca ccgcttcttc agccaccagc ctgacctcaa
     1981 ctacgacagc gccgccgtcc gccgcgcgat gctcgacgtc atgcgcttct ggctcgaccg
     2041 cggcctggac ggcttccgct gcgacgccgt gccctacctg ctcgagcgcg agggcaccaa
     2101 ctgcgagaac ctgccggaga cgcatggcat cctgaaggag ttccgccgtg tcatcgacca
     2161 ggagtacggc ggcgaccgcg tgctgctggc cgaggccaac cagtggccgg aggacgtgcg
     2221 gccctacttc ggcgagggcg acgagttcca catggccttc cacttcccgc tcatgccccg
     2281 cctctacatg gccatccggc gcgaggagcg gctgcccatc ctcgacatct tcacccacac
     2341 gccggcgatt ccctcccggt gccagtggtg cctgttcctc cgcaaccacg acgagctgac
     2401 gctcgagatg gtgaccagcg aggagcggga ctacatgtac tacgcctacg cccaggaccc
     2461 gcaggccaag ctcaacctcg gcatccggcg gcggctggcg ccgctcatgg acaacgaccg
     2521 gcggcggatc gagctgctca actgcctgct gctgacgctg cccggcagcc cgatcgtcta
     2581 ctacggcgac gagatcggca tgggcgacaa catctacctc ggcgaccgca acggcgtccg
     2641 cacaccgatg cagtggtctc gcgaccgcaa tgccggcttc tcgagcgccg acgagagccg
     2701 gctctacctg ccggtgatcg gagatcccgt gtacggctac caggccgtca acgtggaggc
     2761 gcagtcgaag acgccgacgt cgttgctcaa caccatgcgg cggctcatcg ccgcgcgcaa
     2821 gggctgcgcc gcctttggcc gcggcagcat cgagttcctg cgcccgctca atcagagcgt
     2881 cctcgccttc gtccgcgcgc acggcgacga gaccatcctg gtcgtcgcca acctctcggg
     2941 gcgctcgcag cccgtcagcc tcgacctcgc ccgccaccgc ggcgccgtgc ccgtcgagct
     3001 tctcgggcag acacgcttcc cgccgctgtc ggagcacccg tatttcctgt cgctcggtcc
     3061 gcacggcttc tactggttcc ggctcgagcg tgccgatcgc cagccccccc gctatggcat
     3121 cgaggacgcc gccctctgac gcgctggccg cctggatcgc cgggcggcgc tggttcgcga
     3181 ccaagacgcg gcgcatcagc gccgtggtca tcgaggacgc cgtcccgctc ggcggcggcg
     3241 tgctcgccct cgcccgcgtg acgctcgacg acggcacgga agaccggtac gcgctgccgc
     3301 tgtcgccggc ggcggccggc caggtcgccg acgcgctcga cgacccggcg ttctgccagg
     3361 cgctgctcga cctcgtcggc cgcggcggcg ccgccgaggg cgggcgcggc gagctacgcg
     3421 gcgtgccgac cgcggcgttc ccgtcgccgc cccccggcga cacgacaccg cgacggctcg
     3481 gcggggagca gagcaacacg tcggtggcct tcggcagcac gctcatcctc aagcacttcc
     3541 ggcgcctgtc gccggggctc agccccgagg aggagatcgg ccggttcctc accgagcgcg
     3601 tccggttccc tcacgtgccg gcgctgtgcg gccatctcga gtaccgccgc cgggacggcg
     3661 ggcacttcac gctcggcatc gtgcagacgc tgatcgccgg cgcccgggac gggtgggaat
     3721 ggaccctcga cgcgctcggc gaccccaccc gtcggcgtgc cgtgatcgag atgctggcgc
     3781 gcctgggcgc ggccacggcg gcgctgcacg gcgcgctcgc ctcggccgcc gacgaccccg
     3841 acttcgcgcc ggagcctatc gacgatgccg acgcgggcgc ctgggccggg gcggtgcggg
     3901 cgcagctcgc ggcggccgcc gaggctctga gcgaggcgcg gctcgccgtc gacctgccgg
     3961 tcgacgaggg gctccgcggg ctcgtgggtg ccacgaagat ccgccatcac ggcgacttcc
     4021 atctgggcca gaccctctat cgcgaggcgt cgggcgagcc gttcatcatc gacttcgagg
     4081 gcgagccgct ccggccgctg gccgagcgcc ggcgcaagca cacgccgctg cgcgacgtgg
     4141 ccggcatgct gcgctcgatc gactacgccg ccgcggccgc gcgcccggac ggtgacgggt
     4201 gggcggatgg ctggcgcacc gaagcggcgc gcgcgttcac cgacgcctat cgggcgggag
     4261 cccgcggcgc cccgttcgta cccgcgtccg acgaggcctt cgcgcacgcg gtcgcggtgc
     4321 tggtcgtcga gaaagccgcg tacgagatcg tctacgaggc gaacaaccgg ccggagtggg
     4381 tcgcgatccc gcggcgcggg ctgctcagcg cggcttcggc gctgcgggct cgaggcgctg
     4441 gggcggcgtg aagaccatgg actcggcgat gcgtgcgtcc ttgtcgaaga tgacgaggag
     4501 accgctctcg tagaagaagc tcggcggcat ctgcccccgc ggggaga
//
LOCUS       DATIAZ010000347         4788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1818995, whole genome shotgun sequence.
ACCESSION   DATIAZ010000347 DATIAZ010000000
VERSION     DATIAZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818995"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VLF19_11905"
     CDS             <1..133
                     /locus_tag="VLF19_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94002.1"
                     /translation="IEDFRKGKAQAQKALVGLVMKATAGKANPALVNQLLQEKLARR"
     gene            209..835
                     /locus_tag="VLF19_11910"
     CDS             209..835
                     /locus_tag="VLF19_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSE94003.1"
                     /translation="MKVPALTEVSFRIEKGEFVVVTGPSGAGKTTLLRLLYGDDTPTE
                     GDIEILAQPVTELSRSKIQTLRRSLGVVFQDAKLLPGRTVYENIAFVLRVTGTPRREI
                     TPRAFEALRAVGLSSRAQAYPAQLSQGEAQRASLARAIVRKQPVLIADEPTGNLDETM
                     AGEVLAIIKDIWARGTTVLLATHQTAVAAALGRRKLVLEGGRLVKDEG"
     gene            837..1721
                     /locus_tag="VLF19_11915"
     CDS             837..1721
                     /locus_tag="VLF19_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSE94004.1"
                     /translation="MLGFVLSEAFRDLRRAGRVAVSAVVLIALSLVAVGAFWVLSWNL
                     GRAVAEWRDRVRIIVYLKREAPAEVDALLGRVKAVPEVASASYVSKAEALASLKRVLG
                     KDAGVAEQLPVNPLPASVHVTPTAAGATPDGAHALIERLSALPEAEEVAGGVEWVERL
                     GQWQRLLATIGLGTGAVLALAAILTVTTSTTLVLHVRRQETEIMRLVGAPEAVIRLPL
                     LLQGLIQGLVGAGLALLALRVVHHLAAPRLEPLTSLTIGLPHLEFLAPPAMLTILLAG
                     AVLGAFGGMLARGRRGMA"
     gene            1718..2926
                     /locus_tag="VLF19_11920"
     CDS             1718..2926
                     /locus_tag="VLF19_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSE94005.1"
                     /translation="MTVRTRRPPWWPVVLTGLLLALPAAAQSRREDPLQVEQRRLQET
                     QRQLTRERAKAAEARRRETSVLAELEQTERRLADKQRDVARLDTRVKRVQADIATLRG
                     EIDRLQSQRAGQENALARRLRAMYKIHAQGAALPLILSGDDPVTRMVAVRQLADLAAL
                     DARLIQEYRGTSDKLADRKEREEARRSELAGLKQTAQIERAEVDREAAKRRLLLAKVR
                     DERAYHERMVGELSEAARRLETFVRELQAKQRRVAKAPPTTPGDMPRAGFGGLRGRLP
                     WPADGRIVTAFGPQVHPRFGTRTFRNGVDIEAGEGTQVAAVHAGHVVYTGWFKGYGNL
                     IILDHGSDYYTLYAHVAEIVVKEGDDVRQGQRIGTVGDTGSLAGPRLYFEVRFQGKPL
                     DPTEWLRQRG"
     gene            2941..4188
                     /locus_tag="VLF19_11925"
     CDS             2941..4188
                     /locus_tag="VLF19_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSE94006.1"
                     /translation="MKKAFVIGSLLVVLTLTVGGSAVSKSTDGGTTYEQLRLFTEVLS
                     IVQNQYVDEVPPKELIYSAIKGTLRGLDPHSSFLDPDSYREMQVETSGSFGGLGIEIT
                     LRDDVLTVVAPIEGTPAHRAGLHPGDRIVKIDGLVTKDMQLADAVKRMRGKPGTKVTI
                     GVVREGWTEPKDFEITREQIRVQSVRTHDLGNGVAYVRLRQFQEQTSHDIEQALDRFG
                     KNGMKSLVLDLRNNPGGLLTAAVEVTEKFVADGKLVVYTEGRVRNQNMRFSAHAKKAY
                     SEIPMVVLVNQGSASASEIVAGALQDYGRAVLVGTQTFGKGSVQTIIPLSDGSGLRLT
                     TAKYFTPKGRSIHGKGITPDIVVEVPKPPADQKPAPPSLDPMEDLKKDVQLQRALDAL
                     KAMRVIEQGRPAASQAQARPQNQ"
     gene            4258..>4788
                     /gene="tsaD"
                     /locus_tag="VLF19_11930"
     CDS             4258..>4788
                     /gene="tsaD"
                     /locus_tag="VLF19_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSE94007.1"
                     /translation="MIILGIESSCDETAAAVLADGRRVLADVVASQDAIHAPYGGVVP
                     ELASRRHLEVVVPVVERVLADAGVKLADLDGIAVTRGPGLVGSLLIGLSVAKSLAWVG
                     RTPLVGVNHLEGHVYAAFLTEDPPEHPFLALVVSGGHTALYHAKAPLQYALVGQTRDD
                     AAGEAFDKVAKLLGLGF"
BASE COUNT          725 a         1679 c         1716 g          668 t
ORIGIN      
        1 gatcgaggac ttcaggaagg gcaaggccca ggcgcagaag gccctggtcg ggctcgtcat
       61 gaaggccacg gccggcaagg ccaaccccgc gctcgtgaac cagctcctcc aggagaaact
      121 cgctcgccgg tgaggcgggc cgctataatc gagagcgccc gcgatgattg agtttcaccg
      181 cgtcacgaag atatacgagg tcggaccggt gaaagtccct gccctcacgg aggtctcctt
      241 tcgaatcgaa aagggcgagt tcgtcgtggt caccggcccg agcggcgcgg gcaagacgac
      301 gctgttgcgt ctcctctacg gggacgacac gccgaccgag ggtgacatcg agatcctggc
      361 ccagccggtc accgagctgt cgcgctcgaa gatccagacg ctccgacgtt ccctcggcgt
      421 ggtgttccag gacgccaagc tcctgccggg ccgcaccgtc tacgagaaca tcgcgttcgt
      481 gctgcgcgtg accgggacgc cccgccgcga gatcaccccg cgcgccttcg aagcgctccg
      541 cgccgtcggg ctctcctcgc gcgcgcaggc ctaccccgcg cagctcagcc agggcgaggc
      601 ccagcgcgcg tccctggcgc gcgccatcgt ccgcaagcag cccgtgctga tcgccgacga
      661 gcccaccggg aacctcgacg agaccatggc cggcgaggtg ctggcgatca tcaaggacat
      721 ctgggcgcgc ggcacgacgg tgctgctcgc cacgcaccag acggccgtgg ccgccgcgct
      781 cggccgccgc aagctcgtgc tcgagggcgg ccggctcgtc aaggacgagg gctgacgtgc
      841 tcggattcgt gctgagcgag gcgttccggg acctccgacg tgccggccgg gtcgcggtga
      901 gcgcggtcgt gctcatcgcg ctgtcgctgg tcgccgtcgg cgccttctgg gtgctgtcct
      961 ggaacctcgg ccgcgccgtc gccgagtggc gcgaccgcgt ccggatcatc gtctacctca
     1021 agcgcgaggc gcccgccgag gtggacgcgc tgctcgggcg cgtgaaggcg gtgccggagg
     1081 tggcgtcggc gagctacgtg tccaaggccg aggcgctcgc ctcgctcaag cgcgtgctcg
     1141 gcaaggacgc gggcgtggcg gagcagcttc ctgtcaatcc gctccccgcc tcggtccacg
     1201 tgacgccgac cgcggccggc gcgacgcccg acggggcgca cgcgctcatc gagcggctca
     1261 gcgcgctgcc cgaggccgag gaagtcgccg gcggcgtcga gtgggtcgag cggctcggcc
     1321 agtggcagcg cctgctggcc acgatcggcc tcggtaccgg cgcggtgctg gccctcgctg
     1381 ccatcctgac cgtcacgacg tccacgacgc tggtcctcca cgtgcggcgg caggagaccg
     1441 agatcatgcg gctcgtgggc gcgcccgagg cggtgatccg gctgcctctc ctgctccagg
     1501 ggctgatcca gggcctcgtg ggggccgggc tcgcgctgct ggctctgcgc gtcgttcatc
     1561 atctcgcggc gccccgcctc gagccgctga cgagcctgac catcggtctg ccccacctcg
     1621 agttcctcgc ccctcccgcg atgctgacca ttctcctggc cggggccgtc ctgggcgcgt
     1681 tcggcggcat gctcgcccgg gggcgacggg ggatggcgtg acggtccgga cgcggcggcc
     1741 tccgtggtgg cccgtcgtcc tgaccgggct cctgctcgcg ctgccggcgg cggcccagag
     1801 ccgcagggag gatccgctcc aggtcgagca gcgacggctc caggagaccc agcgtcagct
     1861 cacgcgcgag cgagcgaagg ctgccgaggc gcggcgccgc gagacctcgg tgctcgccga
     1921 gctcgagcag accgagcggc ggctggcgga caagcagcgg gacgtggccc ggctcgacac
     1981 acgggtcaag cgggtgcagg cggacatcgc gacgctccgc ggcgagatcg accgtctgca
     2041 gagccagcgc gccggccagg agaacgccct cgcccggcgg ctgcgcgcca tgtacaagat
     2101 tcacgcccag ggcgcggccc tgcctctgat cctgagcggc gacgatcccg tcacgcgcat
     2161 ggttgccgtg cggcagctcg ccgacctggc cgccctggac gctcgtctca ttcaagagta
     2221 tcgtggtaca agtgacaagc tggcggaccg caaggaacgg gaggaggccc gtcgttccga
     2281 gctggcaggc ttgaagcaaa cggcccagat cgagcgggcc gaggtcgatc gcgaagcggc
     2341 caagcggcgg ttgttgctgg ccaaggttcg cgacgaacgc gcctaccacg agcggatggt
     2401 cggagagctc agcgaggccg cccggcggct cgagacgttc gttcgcgagc tccaggccaa
     2461 gcagcggcgc gtcgcgaagg cgccgccgac gactcccggg gacatgcccc gcgcggggtt
     2521 cggcgggctc cggggacggc tcccgtggcc ggccgacggc cggatcgtga cggccttcgg
     2581 tccgcaggtg catcctcggt tcgggacgcg gacgttccgc aacggggtcg acatcgaggc
     2641 gggtgagggc acgcaggtcg ccgcggtgca cgcgggacac gtggtctaca ccgggtggtt
     2701 caagggttac ggcaacctga tcatcctgga ccacgggagc gactactaca cgctctacgc
     2761 ccacgtcgcc gagatcgtcg tgaaggaagg cgacgacgtg cgccagggac agcggatcgg
     2821 gacggtgggg gacacgggct cgctggccgg gccgcgcctc tacttcgagg tgcggttcca
     2881 gggcaagccg ctggacccga ccgagtggct ccgtcaacgc ggatgagggg gacagggtcc
     2941 atgaagaagg cgttcgtgat cggctcgttg ctcgtcgtgc tcaccctgac cgtcggcggt
     3001 tccgccgtca gcaagagcac ggacggcggc acgacgtacg agcagctccg gctcttcacc
     3061 gaggtgctct cgatcgtgca gaaccagtac gtcgacgagg tgccgccgaa ggagctgatc
     3121 tacagcgcca tcaagggcac gctgcgcggg ctcgacccgc actcctcgtt cctcgatccc
     3181 gacagctacc gggagatgca ggtggagacg tccggcagct tcggcggcct cggcatcgag
     3241 atcacgctgc gggacgacgt gctcaccgtc gtggccccga tcgagggcac gccggcgcac
     3301 cgcgcggggc tgcaccccgg ggaccggatc gtgaagatcg acggcctcgt caccaaggac
     3361 atgcagctcg ccgacgccgt caagcgcatg cgtggcaagc ccggcacgaa ggtcacgatc
     3421 ggcgtggtgc gcgagggctg gaccgagccc aaggatttcg agatcacgcg cgagcagatc
     3481 cgcgtgcagt ccgtgcgcac ccacgacctg ggcaacggcg tggcgtacgt ccgcctgcgc
     3541 cagttccagg agcagacgtc gcacgacatc gagcaggcgc tcgaccggtt cggcaagaat
     3601 ggcatgaagt cgctggtcct cgacctgcgc aacaaccccg gcggcctgct caccgccgcc
     3661 gtcgaggtga ccgagaagtt cgttgccgac ggcaagctcg tcgtctacac cgagggacgt
     3721 gtgcgcaacc agaacatgcg gttctccgcg cacgccaaga aggcctacag cgagataccc
     3781 atggtggtgc tcgtcaacca gggcagcgcc tcggcgtcgg agatcgtcgc cggcgcgctg
     3841 caggactacg gtcgcgcggt cctggtcggc acccagacgt tcggcaaggg atcggtccag
     3901 acgatcatcc ccctgtccga cggctcgggg ctccggctga cgacggccaa gtacttcacc
     3961 cccaagggcc ggtcgatcca cggcaagggc atcacgccgg acatcgtcgt cgaggtgccg
     4021 aagccccccg ccgaccagaa gccggcgccg ccgtcgctcg acccgatgga ggacctcaag
     4081 aaggacgtgc agctgcagcg ggcgctcgac gccctcaagg cgatgcgcgt gatcgagcag
     4141 ggacgtccgg ccgcctcgca ggcgcaggcc cgtccgcaga accagtagcg accgtcgcgg
     4201 gcgtggggcg gtgagcgagc tcaccgcccc tcgtctttcg ccacgggtcc ggcaggcatg
     4261 atcatcctcg gcatcgagag ctcgtgcgac gagacggcgg cggcagtgct cgccgacggg
     4321 cgtcgggtcc tcgcggacgt cgtcgcgtcg caggacgcca tccacgcccc ctacggcggc
     4381 gtggtcccgg agctggcctc ccggcgccac ctggaggtcg tcgtgccggt tgtcgagcgc
     4441 gtcctggccg acgccggcgt caagctcgcc gacctcgacg gtatcgcggt gactcggggg
     4501 cccgggctcg tcggctcgct cctgatcggc ctgtcggtcg ccaagtcgct cgcctgggtg
     4561 ggacgcacgc cactcgtcgg cgtgaaccac ctggaaggcc acgtctacgc cgcgttcctc
     4621 accgaggatc cgccggagca ccccttcctc gcgctcgtcg tgtcgggcgg ccacaccgcg
     4681 ctgtatcacg cgaaggcgcc gctgcagtac gcgctggtcg ggcagacgcg ggacgacgcc
     4741 gccggcgagg cgttcgacaa ggtcgcgaag ctgctgggac tcggcttc
//
LOCUS       DATIAZ010000348         5027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_109922, whole genome shotgun sequence.
ACCESSION   DATIAZ010000348 DATIAZ010000000
VERSION     DATIAZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109922"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..318)
                     /locus_tag="VLF19_11935"
     CDS             complement(52..318)
                     /locus_tag="VLF19_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94008.1"
                     /translation="MPRRPRIVPLRPARRAAAESGLVEVYRCPRYEAVVVVSLLESEG
                     IPAVQRSRIAHSVHPFSVGDQGEVVILVPSGEAARARRRLVRLA"
     gene            412..1689
                     /locus_tag="VLF19_11940"
     CDS             412..1689
                     /locus_tag="VLF19_11940"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HSE94009.1"
                     /translation="MADIEYFRGLPEVGIVPRAPHAGPLLEHLYEQEHGRGGFSGRVS
                     HTYHLYPPTNWLPGETRIVEEFAPGWESPLRPLGGVHHALSAAPASDADVYRGLGRLV
                     ANATAAMNVTAPRASMDYFFEHHSATMVFFVHRGAGTLDTTFGPVAYGPGDFLVVPKG
                     VTHRFELAAGPHYYWMYESFAGDPEKSEAPTTGRFLTHSESDYHYPRSLQTVNEQGRF
                     EIVSKVDGVYTRRVHPTHPFDAVGWRGDYLPYKLAVDDVRPLVADRSHVPPSGHTVFR
                     LPGCYLCVFTVRSVEKDAMWVPFFHKNLDYCETIGFHFGDFFSAGGQSQAGLVTVHPV
                     GLPHGPKPRALQAFLDGQRPEVHNEVAVMADFANPTRISDLALGLSRPGYMGAWSAYA
                     TEARFTYDPARLDDVRALGDRLSGARDRLTPPR"
     gene            1689..2714
                     /locus_tag="VLF19_11945"
     CDS             1689..2714
                     /locus_tag="VLF19_11945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HSE94010.1"
                     /translation="MRFGVFSVCDHYPAELPRTSGQLYGELLEQVEAAEDLGFDSFWV
                     AEHHFHEYGAVPAPPVFLAAAAQRTRRIRLGAAVVVLPFHNPLQVAEDYAMVDMLSAG
                     RLDLGAGSGYLRHEYDGFGIDPDEKRERFDEALEVVLRAWSGERFSYEGRFHRVKDVK
                     LNVTPVQRPRPPVWIAILRNEAAAQVGKKGFAPMMIPYVTSERLEEFAETLAAFRTAY
                     AEAGHDPARARLPFGFHTYVTDSLEAATAEARPAMERYVRTRLYARQRPYETLIDRHL
                     IAFGSPDDVVRVARMYEACGYTDYLAIMNFGGLPHASVLRSMERLAKHVLPAFAADAV
                     PRCSGRA"
     gene            2728..3630
                     /locus_tag="VLF19_11950"
     CDS             2728..3630
                     /locus_tag="VLF19_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94011.1"
                     /translation="MSGIVVSCPCGEVYELKPEYAGRLLACPVCGHHLRAGGALPRRV
                     AGADLDPAFDRDVFLLRERVLTIAAKYEVWAENGASILYVERPTYPLRTLLAYGLAAV
                     AALIVMGWTARAARGTDEAVGAFVVLAGWALTVAVFLVVSMSARPLRHVTVYRDESRR
                     EVLLRILQDQRLAFFVRSYTVLTAGGTMLARLRKNYLHNVFRKRWYVEMPPGRKLAMA
                     IEDSIVLSLLRRVLGSFFGVLRTNFVLVHLVGPDAGTAFGEFNRKFTLFDRYALDLSG
                     DPERRLDRRIALAFGVMLDTGERR"
     gene            3740..4486
                     /locus_tag="VLF19_11955"
     CDS             3740..4486
                     /locus_tag="VLF19_11955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSE94012.1"
                     /translation="MTEGEPEQPLRITPDMLLGRRVDFERRMRRAPPVTVGLIIVLTG
                     VFLIELAVGALDSREAMIAAGALEANAVAAGEYWRLVAATLLHGGPDHLFGNAVALFI
                     LGMVCEHAFGWRQFLLLFTASGLSGSVVSLLSGAGPSVGASGAIFGLQAAAIVLFRRH
                     RDRLLMRDRRIGFVLLVWAIYAIASGIRDPYVDNGAHIGGALGGALVGRRLHAVVLAP
                     MTPERAARLRWKTWALWALLAYTLAGWVIR"
     gene            complement(4556..>5027)
                     /locus_tag="VLF19_11960"
     CDS             complement(4556..>5027)
                     /locus_tag="VLF19_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94013.1"
                     /translation="PAPADVDEARVERSAELLELSELAGWFLEPEAVQSDAVDRLQAR
                     GSRLVVSDQLRGEREEAILRRVVEREFAPETRRRWARRLLETALVLDAVDREEHAGLA
                     RAAAAALLDDDREAWRIPFARALASRALDVAGEVSAGRLRAAEVSRKPAPRPPT"
BASE COUNT          659 a         1846 c         1783 g          739 t
ORIGIN      
        1 gacgccccgt gagggacaac gagcctccga ctcgtgagag gccgggctcg gtcaggccag
       61 gcgcacgagc cggcgccgcg cccgcgcggc ctcgccgctc ggcaccagga tcacgacttc
      121 gccctggtcg ccgaccgaga acggatggac ggaatgcgcg atccgcgagc gctggacggc
      181 ggggatcccc tcgctctcga gcaggctcac gaccacgacg gcctcgtagc gcgggcagcg
      241 atagacctcg acgaggccgg actcggcggc ggcgcggcgg gcggggcgca gcgggacgat
      301 acggggacgg cgcggcatga cgcgccccag tgtacaggac gcgcgagcgg ccgtgcgggc
      361 cctgctcctc gacggccgag ggagtacaat tccggagcac aggaggcgcc catggccgac
      421 atcgagtact tccgggggtt gcccgaggtc ggcatcgtcc cgcgcgcgcc gcacgccggc
      481 ccgctgctgg aacacctcta cgagcaggag cacggccgcg gcgggttcag cggccgggtc
      541 agccacacct accatctcta cccgcccacg aactggctgc ccggcgagac gaggatcgtc
      601 gaggagttcg cgccgggctg ggagtcgccg ctccggccgc tcggcggcgt gcaccacgcg
      661 ctgagcgcgg cgcccgcgtc cgacgccgac gtgtaccgcg gcctcggccg gctcgtggcg
      721 aacgccacgg cggccatgaa cgtgacggcg ccgcgcgcgt cgatggacta cttcttcgag
      781 caccactcgg cgacgatggt gttcttcgtc caccgcggcg cgggcacgct cgacacgacg
      841 ttcggcccgg tcgcctacgg ccccggcgac tttctcgtcg tgccgaaggg cgtcacccac
      901 cgcttcgagc tggcggccgg cccgcactac tactggatgt acgagtcgtt cgccggcgat
      961 cccgagaagt ccgaggcacc gacgacgggg cgcttcctca cccacagcga gagcgactac
     1021 cactatcccc gatcgctcca gacggtgaac gagcaggggc gcttcgagat cgtctccaag
     1081 gtggacggcg tctacacacg gcgcgtgcat cccacccatc ccttcgacgc cgtcggctgg
     1141 cgcggcgact acctgccgta caagctcgcc gtggacgacg tccggccgct cgtggccgac
     1201 cgctcccacg tcccgccgtc cggccacacc gtattcaggc tgcccggctg ctacctctgc
     1261 gtcttcacgg tgcgctcggt cgagaaggac gccatgtggg tgccgttctt ccacaagaac
     1321 ctggactact gcgagacgat cggcttccac ttcggggact tcttcagcgc gggcggtcag
     1381 agccaggccg gcctggtgac cgtgcatccg gtggggctgc cccacggccc caagccgcga
     1441 gcgctccagg cgttcctcga cggccagcgg cccgaggtcc acaacgaggt ggccgtcatg
     1501 gcggacttcg ccaacccgac gcggatctcc gatctcgcgc tgggcctgag ccggccgggc
     1561 tacatggggg cgtggtccgc gtacgcgacc gaggcgcggt tcacgtatga cccggcgcgc
     1621 ctcgacgacg tgcgcgcgct cggggaccgg ctgtccgggg cccgcgaccg cctgacgccg
     1681 ccccgctgat gcgcttcggc gtcttctcgg tctgcgatca ctaccccgcc gagctgccgc
     1741 gcacctccgg ccagctctat ggcgagctgc tcgagcaggt cgaggcggcc gaagacctcg
     1801 gcttcgactc cttctgggtg gccgagcatc acttccacga gtacggcgcc gtcccggcgc
     1861 cgccggtgtt cctcgccgcc gcggcccagc ggacgcgccg catccgcctg ggggcggccg
     1921 tcgtcgtgct gccgttccac aacccactgc aggtggccga agactacgcg atggtcgaca
     1981 tgctctcggc cggccgcctc gaccttggcg ccggctccgg ctatctgcgc cacgaatacg
     2041 acggcttcgg catcgacccg gacgagaagc gcgaacggtt cgacgaggcg ctggaggtcg
     2101 tgctgcgcgc gtggtcggga gagcgcttct cgtacgaggg gcgcttccac cgcgtgaagg
     2161 acgtcaagct caacgtgacg cccgtgcagc ggccgcgccc gccggtgtgg atcgccatcc
     2221 tgcgcaacga ggcggcggcc caggtcggca agaagggctt cgcgcccatg atgatcccgt
     2281 acgtgacctc ggagcggctc gaggagttcg cggagacgct cgccgccttc cgaaccgcat
     2341 acgccgaggc cgggcacgac ccggcgcgag cgcggctgcc cttcggcttc cacacctacg
     2401 tcaccgacag cctcgaggcg gcgacggcgg aggcccgtcc cgcgatggag cgctacgtgc
     2461 gcacgcggct ctacgcgcgc cagcgtccgt acgagaccct gatcgaccgg cacctcatcg
     2521 ccttcggcag ccccgacgac gtcgttcgcg tggcgcgcat gtacgaggcg tgcggctaca
     2581 cggactatct ggccatcatg aacttcggcg gcctgccgca cgcctccgtc ctcagatcga
     2641 tggaacggtt ggcgaagcac gtcctaccgg ccttcgcggc cgatgccgtc ccgcgctgct
     2701 ccggccgcgc ctgacgcccc acgcgacgtg agtggcatcg tcgtgtcctg cccgtgcggg
     2761 gaggtgtacg agctcaagcc ggagtatgcc gggcgcctgc tcgcgtgccc cgtgtgcggc
     2821 catcacctgc gagcgggggg cgccctgccg cggcgggtgg ccggggccga cctcgatccg
     2881 gcgttcgacc gcgacgtgtt cctgctgcgc gagcgcgtgc tgaccatcgc cgcgaagtac
     2941 gaggtgtggg cggagaacgg cgcctccatc ctctacgtcg agcgcccgac ctatccgttg
     3001 cggactctgc tggcgtacgg gctggccgcc gtggcggcgc tgatcgtgat ggggtggacg
     3061 gcgcgcgcgg cgcggggcac cgacgaggcg gtcggggcct tcgtggtcct cgccggctgg
     3121 gcgctgacgg tggcggtgtt cctggtcgtg tccatgtcgg cccgcccgct gcgccacgtc
     3181 accgtctacc gcgacgaatc gcgccgcgag gtgctgctgc gcatcctgca ggaccagcgc
     3241 ctggccttct tcgtgcgctc gtacaccgtg ctcaccgccg gtggcacgat gctggcgcgc
     3301 ctccggaaga actacctgca caacgtcttc cgcaagcgct ggtacgtcga gatgccgccg
     3361 ggtcggaagc tggccatggc catcgaggac tcgatcgtgc tgtcgctgct gcgacgggtg
     3421 ctcggctcgt tcttcggagt cttgaggacg aacttcgtgc tggtgcatct cgtcggcccc
     3481 gatgccggga cggcgttcgg ggagttcaac cgcaagttca ccctgttcga ccggtatgca
     3541 ctcgatctct ccggcgaccc cgagcgccgc ctcgaccggc gcatcgcgct ggcattcggt
     3601 gtgatgctcg acaccggcga gcgccggtga cggcgccggt gcgcaccgtc gacgccgccg
     3661 aggcggaggc ctgcgtcgcg acgcccgacg acctcctgga cgacgccgac ggcacccacg
     3721 acgagccggt gggggcgcga tgacggaggg ggagcccgag cagccgctgc gcatcacgcc
     3781 cgacatgctg ctcggccggc gcgtggactt cgaacggcgc atgcggcgcg cgccgccggt
     3841 cacggtcggg ctcatcatcg tgctgaccgg ggtcttcctg atcgagctgg cagtgggtgc
     3901 cctcgactcc cgcgaggcga tgatcgccgc cggcgccctc gaggcgaacg cggtcgcggc
     3961 gggcgagtac tggcgcctcg tcgccgcgac cttgctgcac ggcggccccg atcatctctt
     4021 cggcaacgcg gtggcgctct tcatcctggg catggtgtgc gagcacgcgt tcggctggcg
     4081 gcagttcctc ctgctcttca cggccagcgg gctctccggc tcggtcgtca gtctcctgtc
     4141 gggcgccgga ccctccgtgg gcgcgtccgg cgccatcttc ggcctgcagg cggcggcgat
     4201 cgtgctgttc cgtcgccacc gcgaccgcct gctgatgcgc gaccggcgca tcgggttcgt
     4261 gctcctggtg tgggcgatct acgctatcgc cagcggcatc cgcgacccgt acgtggacaa
     4321 cggcgcgcac atcgggggtg cgctcggcgg cgcgctggtc ggccgccggc tgcacgccgt
     4381 ggtcctggcc ccgatgacgc cggagcgagc ggcccggctc cgctggaaga cgtgggcgct
     4441 ctgggcgctc cttgcttaca ccctggccgg ctgggtgatt cgctagccaa gcggcgccag
     4501 gcctcgacgg ctccctccgc cccacaggtt ccattgcggc ggcagccagg tgcggtcagg
     4561 tcggggggcg tggggcaggc ttgcggctga cctcggcggc gcggagccgc cccgccgaga
     4621 cctcgccggc gacgtcgagc gcccgggagg cgagcgcccg cgcgaacggg atccgccacg
     4681 cctcgcgatc gtcgtccagc agcgcggccg cggcggcacg cgccaggccc gcgtgctcct
     4741 cgcgatcgac ggcgtcgagg acgagggctg tctccagcag acgccgcgcc cagcgccggc
     4801 gcgtctcggg agcgaactcg cgctccacga cgcgccggag gatggcctcc tcccgctcgc
     4861 cgcggagctg gtcggagacg acgagacggc tcccgcgcgc ctggagacga tccacggcat
     4921 cgctctgaac cgcctcgggc tcgaggaacc agccggccag ctccgacagc tcgagcagct
     4981 ctgcggatcg ctcgacgcgc gcctcgtcca cgtccgctgg cgcaggg
//
LOCUS       DATIAZ010000349         5482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1425910, whole genome shotgun sequence.
ACCESSION   DATIAZ010000349 DATIAZ010000000
VERSION     DATIAZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425910"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VLF19_11965"
     CDS             <1..1293
                     /locus_tag="VLF19_11965"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSE94014.1"
                     /translation="NFALERLVDAAAQELGLDAVEIRRKNFIQPDKFPFKTPCGNVYD
                     SGNYPAVLDKALQMADWPALRRMQAEARTQGRYVGLGLATAHQRSVYGPTEFWFWYDA
                     PGLTSVPESVGLSVGPTGEFVVTMFSPFWGNSPETVVAQTIAEEFGIEPGKVTVTYDD
                     SLHGLPGAGPGGSRMTVMLTGAAVGAARKLKDKMARIAAHLLEAAPEDIVFQNGGAMV
                     RGAPQKVLTYADLGLKAYWFKLDLPQGMESGLEARHTYDHPFLTMPSADRKDLGSFYP
                     MMAHGTHIPVVEVTPVTGQVKILRYIAVHDVGTIVNPRSLRGQIAGGITQGIGMALYE
                     ECVYDEQGQPLSASFMDYLLPTAAETPTFEIGHVETPSPFTEYGVKGGGEGGRMIAPA
                     ALASAVEDALAPLGVRIDELPMTPERLATLVEAAARKG"
     gene            complement(1309..2685)
                     /locus_tag="VLF19_11970"
     CDS             complement(1309..2685)
                     /locus_tag="VLF19_11970"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HSE94015.1"
                     /translation="MADPRANAATAIVLFTRDLRVHDHPALAKATALAERVVPLFVLD
                     EALLGSDFACPNRLKFLIESLNDLEGSLRALGGALAVRRGDTVHEVVAVARSVGARAV
                     LASADVSAYARRRERRLGAACAEAGIELGLFPGVTVVRAGVLTPAAGDHFRVFTPYWN
                     RWRTEPLRSLARRPRRIVLPRGLDRGRLPGLQELTTASPSAALPTGGESAARARLDAW
                     LRSGLACYAERHDELAGDGTSRLSPYVHFGCLSPVEVARRAADRPGGEAFLRQLCWRD
                     FHHQVTAAFPAIARQDYRPRGDAWDDDASLLAVWKEGRTGYPVVDAGMRQLRAEGWMH
                     NRARLVTASFLVKDLYLDWRLGAAHFFDLLVDGDVACNAGNWQWVAGTGNDTRPNRVL
                     NPVRQAQRFDPQGDYVRRWVPELREVRGARVHEPWLLEAGTRRRLDYPERIVDHAAAA
                     ATFRGHRR"
     gene            complement(2678..4156)
                     /locus_tag="VLF19_11975"
     CDS             complement(2678..4156)
                     /locus_tag="VLF19_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE94016.1"
                     /translation="MSELPVLVTGATGYVGGRLVPRLLDAGYRVRCLARSAPKLAARP
                     WAGDPRVEIVEVDLTDPDATASAMLECRAAYYLVHSMVSTGMRYAEADRQLAHRFATA
                     AARASVGRIIYLGGLGERGVGLSEHLASRREVEAVLAAGPVPVTVLRAAMIIGSGSAS
                     FEILRYLVERLPVMVTPRWVKTASQPIAVSNVLSYLVACLSVPATIGRTLDIGGPDVV
                     SYLDLMRIMAEKRGLRRRIVIPVPVLTPRLSSLWIQLVTPVSRQIARPLAEGLRNPVV
                     CRDDEAARLMPQRLLGVREAIRVALDQVAHREVETGWFDAGPIPGDPDWAGGTVFTDR
                     REVEVAAPPADVFAAVSRIGGETGWYGADWLWRIRGALDRLAGGPGLRRGRRDAQRVA
                     FGDAIDFWRVTAIEPGRRLALRAEMRLPGDALLEFAVEPSPGRPDRCRLVQTARFLPR
                     GLLGLAYWYAMLPGHVVVFGRMLEGSRGAAEALAAKRDPIVG"
     gene            complement(4643..>5482)
                     /locus_tag="VLF19_11980"
     CDS             complement(4643..>5482)
                     /locus_tag="VLF19_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSE94017.1"
                     /translation="TGASGYIAQRMFRELDERWTLVPIDVRPTTRDGSTVPGIVIADL
                     TRPDRDDYRRHFRGADAVIHCGYVSAPGLDATTWQDNSDAKFWAEHQNVALAYNVCKT
                     ALEEGVRRVVVASSNHAADYYERLVWAGRLEMVTPDMQPRSDNWYGWAKAAYELLGFV
                     FAAGQVGGRRLEIVQWRIGGPRDDDIDRVKPGDVKGMHRALGAYLSRRDQVQQAIRMV
                     ETEDITDEHGVPFLIVYGISGNTHRFWSIANARTKLGYTPEDDSQVNFANKIAAIARA
                     ARR"
BASE COUNT          856 a         1981 c         1925 g          720 t
ORIGIN      
        1 aacttcgccc tcgagcgcct cgtggacgcc gccgcgcagg agctcggcct ggacgccgtc
       61 gaaatccgga gaaagaactt catccagccc gacaagttcc cgttcaagac gccgtgcggc
      121 aacgtctacg acagcggcaa ctatcccgcc gtgctcgaca aggcgttgca gatggccgac
      181 tggccggcgc tccgccgcat gcaggccgag gcccgcacgc aaggccgcta cgtcggtctc
      241 gggctggcga cggcgcatca gcggagcgtc tacgggccga ccgagttctg gttctggtac
      301 gacgcgccgg ggctcacgag cgtcccggag agcgtcggcc tcagcgtcgg gccgacgggc
      361 gagttcgtcg tgacgatgtt ctcgccgttc tggggcaaca gcccggagac ggtcgtcgcc
      421 cagaccatcg ccgaagagtt cggcatcgag cccgggaagg tgaccgtgac gtacgacgac
      481 tcgctgcacg ggctgccggg cgcgggcccg ggcggcagcc ggatgaccgt catgctgacc
      541 ggcgcggccg tcggcgccgc gcgcaagctc aaggacaaga tggcccgcat cgcggcgcac
      601 ctgctcgagg ccgcgcccga ggacatcgtc ttccagaacg gcggcgcgat ggtccgcggc
      661 gcgccgcaga aggtgctcac ttacgccgac ctcgggctga aggcctactg gttcaagctc
      721 gacctgccgc agggcatgga gagcgggctc gaggcgcgcc acacctacga ccatcccttc
      781 ctgacgatgc cgtcggcgga ccgcaaggac ctcggctcct tctacccgat gatggcgcac
      841 ggcacgcaca tcccggtcgt ggaggttacc ccggtgacgg ggcaggtgaa gatcctccgc
      901 tacatcgccg tccacgacgt cggcaccatc gtgaacccca ggagcctgcg ggggcagatc
      961 gcgggcggca tcacccaggg catcggcatg gctctctacg aggagtgcgt gtacgacgag
     1021 caggggcagc cgctgtccgc gagcttcatg gattacctcc tgccgacggc cgccgagacg
     1081 ccgacgttcg agatcggcca cgtcgagacg ccgtcgccgt tcacggagta cggggtcaag
     1141 ggcggcggtg aaggcggacg catgatcgcg ccggcggcgc tggccagcgc cgtcgaggat
     1201 gcgctggcgc ccctgggggt caggatcgac gagctcccga tgacgccgga gcggctcgcg
     1261 acgctcgtcg aggcggcagc gcggaagggc tagccaaggc agatccgctc agcgccgatg
     1321 cccgcggaat gtggcggccg ccgccgcgtg gtcgacgatg cgctccggat agtcgaggcg
     1381 gcgccgtgtg cccgcctcga gtaaccatgg ctcgtgcacg cgcgcgccgc gcacctctcg
     1441 cagctcgggc acccagcgcc tgacgtaatc gccctgcgga tcgaagcgct gcgcctgccg
     1501 caccggattg aggacgcgat tcggccgcgt gtcgtttccg gtgcccgcca cccactgcca
     1561 gttgccggcg ttgcaggcga cgtcgccgtc cacgagcaga tcgaagaagt gcgcagcgcc
     1621 gagccgccag tccagataga gatccttcac caggaaggag gccgtcacca gtcgtgcccg
     1681 gttgtgcatc cagccctccg cgcgaagctg acgcatgccg gcgtccacga ccgggtagcc
     1741 ggtacgaccc tctttccaca cagccagcaa gctcgcgtcg tcgtcccacg catcgccgcg
     1801 cggccggtag tcctgccgtg cgatggccgg gaacgccgcc gtcacctgat ggtggaagtc
     1861 tcgccagcac agctggcgga ggaacgcctc gccgccaggc cgatcggccg cgcggcgcgc
     1921 cacctcgact ggcgagagac agccgaagtg cacatacggg ctgaggcgcg aggtgccatc
     1981 gccggcgagc tcgtcgtgac gctcggcgta gcacgccaga ccgctgcgaa gccaggcgtc
     2041 cagccgggcg cgggccgccg actcgccacc ggtggggagc gcggccgagg gggaggcggt
     2101 ggtcagctcc tgcaatccgg ggaggcggcc ccggtcgagt ccgcgcggca ggacgatgcg
     2161 ccgcggccgc cgcgcgagcg accggagcgg ctccgtgcgc cagcggttcc agtagggcgt
     2221 gaagacgcgg aagtggtcgc cggccgcggg cgtgagcacg ccggctctga ccaccgtgac
     2281 gcccggaaac agcccgagct cgatccccgc ctccgcgcag gcagcgccca gacggcgctc
     2341 acgccgccgg gcgtacgcgc tcacgtcggc gctcgcaaga acggcccgtg cccccaccga
     2401 ccgcgcgacg gcgactacct cgtgcaccgt gtcgccgcgg cggacggcca gcgcgccgcc
     2461 cagcgcccgc aacgacccct cgaggtcgtt cagtgactcg atcaggaact tcagccggtt
     2521 cgggcacgcg aagtcgctgc cgagcaacgc ctcgtcgagc acgaagagag gcaccacgcg
     2581 ctccgcgagg gccgtcgctt tcgcgagcgc gggatggtcg tgcacccgga ggtcgcgcgt
     2641 gaacagcacg atcgccgtgg cggcgttagc gcgcgggtca gccaacgatc gggtcccgtt
     2701 tcgccgccag agcctcggcg gcaccgcggc tgccctcgag catccgaccg aacacgacga
     2761 cgtgacccgg cagcatggcg taccagtagg cgaggccgag caggccgcgg ggcaggaagc
     2821 gcgcggtctg gaccaggcgg cagcggtcgg ggcgcccggg cgatggctcg accgcgaact
     2881 ccaggagcgc atccccgggc aaccgcatct ccgcgcgcag cgccagccgc cgccccggct
     2941 cgatggccgt cacacgccag aagtcgatgg cgtccccgaa cgccacccgc tgggcatccc
     3001 ggcgcccccg ccggagcccc ggtccgccgg ccagacggtc gagggcgccg cggatccgcc
     3061 agagccagtc cgcgccgtac cagccggtct ccccgccgat ccttgacacg gccgcgaaca
     3121 cgtcggccgg gggcgccgcc acctcgacct cgcgccgatc ggtgaagacc gtgccgcccg
     3181 cccagtcggg atcgcccggg atcggcccgg catcgaacca gccggtctcg acctcgcggt
     3241 gcgccacctg gtcgagcgcc acgcggatcg cctcgcgcac gccgagcagc cgctgcggca
     3301 tcaggcgcgc cgcctcgtcg tcgcgacaga ccaccgggtt ccgcagcccc tcggcgagcg
     3361 gccgggcgat ctgccgactg acgggggtga cgagctggat ccacagcgag ctcagccgtg
     3421 gggtgaggac cggcacggga atgacgatcc ggcgccgcag gccacgcttc tcggccatga
     3481 tccgcatgag gtccaggtag ctgacgacgt ccggcccgcc gatgtccagc gtccgaccga
     3541 tcgtcgcggg cacggacagg cacgccacga ggtagctcag gacattgctg acggcgatcg
     3601 gctgcgacgc ggtcttgacc caccgtggtg tcaccatcac cggcagccgc tcgacgaggt
     3661 agcgcaggat ctcgaaggag gccgagccgg agccgatgat catcgctgcg cggaggacgg
     3721 tgacgggcac cggccccgcg gccaggacgg cttccacttc ccttcgggac gccaggtgct
     3781 cgctcagccc gactccccgc tcgccgaggc cgcccaggta gatgatgcgg ccgacgctgg
     3841 cgcgcgcggc ggccgtggcg aagcggtgcg cgagctgccg gtcggcctcg gcgtaccgca
     3901 tccccgtcga gaccatcgag tgcacgaggt agtaggcggc gcggcactcc aacatcgcgg
     3961 acgcggtggc gtcggggtcg gtgagatcca cctccacgat ctcgacgcgc gggtcccccg
     4021 cccagggacg ggcggcgagc ttgggcgccg agcgcgcgag gcaccgcacc cggtacccgg
     4081 cgtcgagcag ccgcggcacc agacggccgc cgacgtatcc ggtggccccc gtgacgagga
     4141 ccggcagctc gctcatgctc cgccccgggt ccgaatgcag ccgggcccgc ggtcgacgcc
     4201 gagagcccgc gtcctgaccc ggcgctcctc gaagtccagg agccgggctg cggcggcgac
     4261 cgcgatgacg cgcgccacgc cccgatcgag ggtcagcaat gtgaacgcga ggccgacttg
     4321 cagcgcatca cgcccggcgc gcggacggtg cgcgcggcgg gagagtcgat tcattcctac
     4381 gagggatccc gtccggccgg ttctccgtcc agacgctccc cctcgacgcg acgatgccat
     4441 cactcccaga ctcccgagca gaagcacggg aaaaatgccg aggtacgtga agttgtcgac
     4501 gaacgcttga agtgcctcca tggcgtccct cgggtctacg tgatacgaac ccgtgttcgc
     4561 aagaccagca ctatgatcga ccacgcaaag agccgagacc acacccctct tggtggcccc
     4621 caggccggcg cgtgcaggcg gctcatcggc gggccgcccg cgcgatcgcc gcgatcttgt
     4681 tggcgaagtt cacctgactg tcgtcctccg gcgtgtagcc gagcttcgtg cgcgcattgg
     4741 cgatggacca gaagcgatgg gtgttcccgc tgatgccgta gacgatcagg aacggcacac
     4801 cgtgctcatc ggtgatgtcc tcggtctcga ccatccggat ggcctgctgc acctggtcgc
     4861 gtcgcgagag ataggctccc agggcccggt gcatgccctt cacgtcgccg ggcttcaccc
     4921 gatcgatgtc gtcgtcgcgg ggccccccga tccgccactg gacgatctcc agccggcggc
     4981 cgccgacctg gccggcagcg aagacgaacc ccagcagctc gtaggcggcc ttcgcccagc
     5041 cgtaccagtt gtcggagcgc ggctgcatgt cgggcgtgac catctcgagc cggccggccc
     5101 agacgagacg ctcgtagtag tcggcggcat gattggagct ggcgacgacc acccggcgga
     5161 cgccctcttc cagcgctgtc ttgcagacgt tgtaggccag cgcgacgttc tgatgctcgg
     5221 cccagaactt tgcgtcgctg ttgtcttgcc acgtggtcgc gtcgagaccc ggggcgctca
     5281 cgtacccgca gtgaatcacg gcatcggcgc cgcgaaagtg gcggcggtag tcatcgcggt
     5341 ccggccgcgt cagatcggcg atcacgatcc ccgggacggt gctgccgtcc cgggtggtcg
     5401 gacggacatc gatcggcacg agggtccacc gctcgtcgag ctctcggaac atgcgctggg
     5461 cgatgtagcc ggaggccccg gt
//
LOCUS       DATIAZ010000350         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1556832, whole genome shotgun sequence.
ACCESSION   DATIAZ010000350 DATIAZ010000000
VERSION     DATIAZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556832"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..475
                     /locus_tag="VLF19_11985"
     CDS             131..475
                     /locus_tag="VLF19_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HSE94018.1"
                     /translation="MSRGRRREGKKREAGGSARGRRWVRHVMETSNAMDLPPGIFTRV
                     PTDIARGLKQSVLRSTRTKGTKFQSAMSMLNLYINRTGRNLGARDRRRLEAAKGELRR
                     VFGREPRPSGRR"
     gene            complement(472..1785)
                     /locus_tag="VLF19_11990"
     CDS             complement(472..1785)
                     /locus_tag="VLF19_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94019.1"
                     /translation="MTKAVALLAVVIAGAASAEAQVLFASRPSPQLAVTPLFIVASVA
                     PGATDVPVEVVFSLMIPPTRSALDFEQDLYLLWPGAVVTAGTTKADFALPPAVAAHVE
                     VIDEGRLRVFAQRHYESAGDVEEVLPGAAFVTVARSGGPLGLTPPATYIRIPWTPKIA
                     NQAWLVSVRLTARGFVKSKPTTWLGRMVSGPRSLLALGFNDIGAPAMFSMYYWQRDRV
                     LPVVSPARLVVNFAGADRLAIDTLFPPAARRELSESLETTEAVSLFLTPAEGVTPQVL
                     RVEFGYFSRLQSWGPILVPALFFALGNVAAVLLRAFVERIRQRLAGRVQVGRRRATAT
                     RVDDGVVIPREQLEAIVPMETTYEEVLRRCGPEVEEREDLAAPRRKVLVYRGRRDVPH
                     RRWAWGWLAAVSYWDVERHEVEITLQDGVVQDIQVRVGRTRHAQP"
     gene            1952..>2278
                     /locus_tag="VLF19_11995"
     CDS             1952..>2278
                     /locus_tag="VLF19_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HSE94020.1"
                     /translation="MSEAPSKPSHFIRDEVVKDLETGRYTQVVTRFPPEPNGYLHIGH
                     AKAICLDFGMAAEFGGRCHLRFDDTNPTKESQEYVDAIVRDIRWLGFDWGRHLYHASD
                     YFEQLYD"
BASE COUNT          375 a          798 c          825 g          280 t
ORIGIN      
        1 gtcggcgcgg cacagcaggc agtccggatc acgggcgaag gggagggcgg ggacgtcggc
       61 ggccacgccc tccaccgtac atcgcttccc ttgttcgagc ggttatcctg gatgcatggc
      121 gtcccgcaag atgagcaggg gcagaaggcg cgagggcaag aagcgcgagg cgggcggctc
      181 cgcgcgcggg cggcgatggg tccggcacgt catggaaacg tcgaacgcca tggacctgcc
      241 acccggaatc ttcacgcgag tcccgacgga catcgcgcgc gggctcaagc agtcggtgct
      301 gcgcagcacg cgcacgaagg gcacgaagtt ccagtccgcg atgtcgatgc tgaacctcta
      361 catcaaccgg acggggcgca acctgggcgc gcgcgatcgg aggcgcctgg aggccgcgaa
      421 gggcgagctg cgccgagtct tcgggcgaga accgcggccg agcggccggc gctagggctg
      481 ggcgtggcgc gtccggccga cgcgcacctg gatgtcctgc acgacaccgt cctgaagcgt
      541 gatctcgacc tcgtgacgct cgacatccca gtagctcacc gccgccagcc atccccacgc
      601 ccaccgccgg tgcgggacgt cgcgccgtcc ccggtagacc aggaccttgc ggcgcggcgc
      661 ggcgaggtcc tcccgctcct cgacctccgg cccgcaccgg cgcagcacct cctcgtacgt
      721 cgtctccatg ggcacgatcg cctccagctg ctcgcgcggg atgacgacgc cgtcgtcgac
      781 ccgggtggcg gtggcccgcc gccggcccac ctgaacgcgc ccggcgaggc gctggcggat
      841 gcgctcgacg aaggcgcgca gcagcaccgc cgccacgttg ccgagcgcga agaacagcgc
      901 gggcacgagg atcgggcccc aggactgcag gcgcgagaag tagccgaact cgacgcgcag
      961 cacctgtggc gtcacgcctt cggcaggcgt gaggaagagc gagacggcct cggtggtctc
     1021 cagcgactcg ctcagctcgc gccgcgcggc cggaggaaac agcgtgtcga tcgcgaggcg
     1081 gtcggcgccc gcgaagttca cgacgaggcg cgcggggctc accaccggca ggacgcggtc
     1141 gcgctgccag tagtacatgg agaacatggc cggcgcgccg atgtcgttga agcccagcgc
     1201 gagcaggctg cgcggcccgg agaccatccg gccgagccag gtcgtgggct tggacttgac
     1261 gaagcccctg gccgtgagac gcacgctcac gagccacgcc tggttggcga tcttcggcgt
     1321 ccacgggatg cggatgtacg tcgcgggcgg ggtgagaccg agggggccgc ccgagcgggc
     1381 gaccgtgacg aacgccgcgc ccggcaggac ctcctcgaca tcgcccgcgc tctcgtaatg
     1441 acgctgcgcg aagacccgca agcgcccctc gtcgatgacc tccacgtgcg cggccaccgc
     1501 gggcggaagc gcgaagtccg ccttcgtggt gccggccgtc acgacggcgc cgggccagag
     1561 caggtacagg tcctgctcga agtcgagcgc gctccgggtc ggagggatca tcaggctgaa
     1621 gacgacctcg acgggaacgt ccgtggcgcc gggcgccacg ctggcgacga tgaagagcgg
     1681 cgtcactgcg agctgggggc tcggacgcga ggcgaagagc acctgggcct cggcgctcgc
     1741 ggcacccgcg atgacgaccg cgagcagggc gaccgctttg gtcacgggcc ggaagcttga
     1801 cacggcgcca gacggtcggc aacggcgccc cggggtcggc cggcgacgct gtgcctggca
     1861 ccagcgtcat cgtacgtcgg cgagcaggag tctccgtcgg agccgttccg tcgccggagg
     1921 ggagggccgc cggggtgata ccatcacagg gatgagcgag gcgccgagca agcccagcca
     1981 cttcatccgc gacgaggtcg tcaaggacct ggagaccggc cgctacaccc aggtcgtcac
     2041 gcggtttccg cccgagccca acggctactt gcacatcggc cacgccaagg ccatctgcct
     2101 cgacttcggg atggctgccg agttcggcgg ccggtgccac ctgcgcttcg acgacaccaa
     2161 ccccaccaag gagagccagg agtacgtcga cgccatcgtg cgcgacatcc gctggctcgg
     2221 cttcgactgg ggccggcacc tctaccatgc gagcgactac ttcgagcagc tctacgac
//
LOCUS       DATIAZ010000351         5730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_241076, whole genome shotgun sequence.
ACCESSION   DATIAZ010000351 DATIAZ010000000
VERSION     DATIAZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241076"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VLF19_12000"
     CDS             complement(<1..393)
                     /locus_tag="VLF19_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94021.1"
                     /translation="MQRIAVTAIGWRAHAALAASGGAARPLGAPSGYLEAGGEIVWLG
                     APGDALHGRAILAKPGRLSGGDVSLDLGAVRPWRGSISRHVPERSTAEALAAAGERLC
                     AALEEVGEPRGFGTLLAGRVPAFPLEAAA"
     gene            464..1666
                     /locus_tag="VLF19_12005"
     CDS             464..1666
                     /locus_tag="VLF19_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSE94022.1"
                     /translation="MRARVVVPMAVLAAATVVAAASDEEREFLRRHWRAPLAPQGPAP
                     ERFSPVEASLHPEACGTCHPAQLADWRTSWHAAATGPGVAGQLVEMIEREPTTALGCL
                     GCHAPLAEQAPLIRVGGALERNPAFDAALLTKGLACAGCHVRAHERFGPPRRDGSVAS
                     AAPRATLPHGGATRTRAFLASEFCRGCHQFEPDGLKLDGKLLENTYEEWRASAFSRRG
                     VQCQDCHMPDRRHLWRGIHDVEMVRSGLTITVRGGAARYRAGDTARLALSVESTRVGH
                     AFPTYVTPKVVLRGELLDAGGAVVPGSVQEVVIAREVSLDLSREVFDTRLLPGRRAVL
                     DYRPHLTGPGLRARLSVVVYPDAFYTRFYEALLAGGAGRGEAAVREALAASRGSVFTV
                     FEQELPLT"
     gene            complement(1632..2435)
                     /locus_tag="VLF19_12010"
     CDS             complement(1632..2435)
                     /locus_tag="VLF19_12010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE94023.1"
                     /translation="MARRALFAALDGVRTALEHASRLPHHAAVGLVRRADLERGREAA
                     WRRFAELQAADATFTLMAWEEEFYGRFLERGDRILVAGCGTGRDLLGLLERGYSAEGL
                     DVVAACVEAAQLNLKRRGYRTPLHVGAIETAELTGVFDAVIFSWYCYSYIPGRRARVE
                     TLRRMRSHLAEGGRVLICYIRRSEPWGAIGPRVARATSWLSRSDWRPEPGDVVAFEQG
                     IASARYEHAFLAEEIEAEARDAGLAVASHDRREEGLLVLTSAAAPARIP"
     gene            complement(2446..4056)
                     /gene="ggt"
                     /locus_tag="VLF19_12015"
     CDS             complement(2446..4056)
                     /gene="ggt"
                     /locus_tag="VLF19_12015"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSE94024.1"
                     /translation="MTTRWSEAHGTRGLVASPHLLASEAGVAVLRRGGNAVDAAVAAA
                     STIAVVYPHMNSIGGDNFWLIHDARRRRLLALNAAGRSAAAVDADAYRARHGETMPTR
                     GGAAALTVPGVVSGWWEAHRLSRETLGSSLAWPDLFQHALVHAREGFPVSPGQQRVTA
                     QAAALFAGDAPAEVRRSFWPIYHPDRFAEGRFTQPALAATLAAVAEGGAEEFYRGGLA
                     RRIAAGAAAAGSPLTARDLAEHTADWVEPLRVPYRGGEATSFPPPTQGFAALAILALL
                     EGFDVARLDDAEHVHLIVEATKLAFEDRDRWLADPDVVAVPVTTCLDPARLARRRARI
                     AAHAAVAPAGVPAEGDTIAIVTADGKGNAVSVIQSTYHEFGAAVVAGDTGVLLQNRGA
                     FFSLDPAHPNALGPRKRTAHTLIPSMYLVGGRPRLVYGTMGGEGQPQTQAALVTRIVD
                     RGLGPQAAVEAPRWLYGRTWGEPSKALRIEDRFGEGVAERLRRRGHDVRVVEAWSDLM
                     GHAHVIRVDADGLAGGSDPRADGAALGC"
     gene            4245..5270
                     /locus_tag="VLF19_12020"
     CDS             4245..5270
                     /locus_tag="VLF19_12020"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010370114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HSE94025.1"
                     /translation="MLTTTIAGSLPKPRWLAAPETLWAPWRLPDDTLREGQRDAVVVA
                     LKLQETAGIDVVTDGEQSRQHFVHGFVAGLAGIDFGKRVTIGIRADRYKAEVPTVTGP
                     IARRAPVHLEDVRWARAHTERRLKFTIPGPMTIVDTLADEHYRDRARLALAFAKVINE
                     EARDLVGAGLDVLQLDEPAFNVYMDDVAAWGIEALHRATEGLGCTTAVHICYGYGIKA
                     NVDWKRTLGSEWRQYEKTFPLLAQSRIDQVSLECANSRVPLELLGLLRGKDVLVGAVD
                     VASLDVESPEQVAATIRRAMAFVPPERLWPCTNCGMVPLPRAVAEGKLRALGAGARLV
                     RAELGAR"
     gene            5419..>5730
                     /locus_tag="VLF19_12025"
     CDS             5419..>5730
                     /locus_tag="VLF19_12025"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSE94026.1"
                     /translation="MRWLVDGYNVIRRDPDLRGAEAAGLEAGRAALLRAIAPVARRSA
                     DRFTVVFDGAPSRAPSPAPGRLEVVFSRPPQKADDVLGRMAREAGAGTVVVSDDRAVA
                     DA"
BASE COUNT          731 a         2174 c         2112 g          713 t
ORIGIN      
        1 cgccgcggcc tcgagcggaa acgccggcac tcgccccgcg agcagcgtgc cgaagccacg
       61 cggctcgccg acttcctcca gcgcggcgca cagccgttcg cccgcggccg cgagcgcctc
      121 cgccgtcgaa cgttcaggga cgtgtcgaga tattgagccg cgccacggac gtaccgcgcc
      181 gaggtccagg ctgacgtctc ctccggagag gcgtcccggc ttcgcgagga tggcgcgccc
      241 gtggagcgca tcgccgggcg ctccgagcca cacgatctct ccgccggcct cgagatagcc
      301 cgacggtgcc ccgagcgggc gcgcggcgcc gcccgaggcc gccagcgcgg cgtgagcgcg
      361 ccagccgatc gcagtcacgg cgatcctctg cacgtcgccg aggctaccac ggggcccgcc
      421 acggccgtcg ctgccggtga cgtcgcctgc taaggtggga cgcatgcgcg cccgcgtggt
      481 cgtgccgatg gccgtgctcg cggccgcgac ggtcgtcgcc gcggccagtg acgaggagcg
      541 cgaatttctc cggcgccact ggcgagcgcc gctggcgccg caagggccgg cgcccgagcg
      601 cttctcgccg gtcgaggctt cgctgcaccc ggaggcgtgc ggcacctgtc acccggcgca
      661 gctcgccgac tggcgcacga gctggcacgc ggcggcgacg gggcccgggg tcgccgggca
      721 actcgtggag atgatcgagc gcgagccgac gacggccctc ggctgtctcg gctgtcacgc
      781 cccgctcgcc gagcaggcgc cgctgatccg tgtgggcggg gcgctcgagc gcaatccggc
      841 gttcgacgcc gcgctgctca ccaagggcct cgcgtgcgcc ggctgccacg tgcgtgccca
      901 cgagcgattc gggccacccc gccgcgacgg ctcggtcgcg agcgccgcgc cccgcgcgac
      961 gctcccgcac ggcggcgcga cgcgcacgcg cgcgttcctc gcctccgagt tctgtcgcgg
     1021 ctgccatcag ttcgagccgg acggcctcaa gctcgacggc aagctgctcg agaacaccta
     1081 cgaggagtgg cgggccagcg cgttctcgcg ccgtggcgtg cagtgtcagg actgccacat
     1141 gccggaccgg cgccacctgt ggcgcggtat ccacgacgtc gagatggtcc gctccggcct
     1201 gacgatcacc gttcgtggag gcgcggcgcg ttaccgcgcc ggcgacacgg cgcgcctcgc
     1261 gctgagcgtg gagagcacgc gcgtgggcca cgccttcccg acgtacgtga cgccgaaggt
     1321 cgtgctgcgc ggcgagctgc tcgacgccgg cggtgcggtc gtgcccggca gtgtgcaaga
     1381 ggttgtgatc gcccgcgagg tgagcctgga cctctcccgg gaggtcttcg acacgcgcct
     1441 cctgcccgga cggcgagcgg tgctcgacta ccggccccac ctgacggggc ccggcctgcg
     1501 cgcccgcctc agcgtggtcg tctatcccga cgccttctac acgcggttct acgaggcgct
     1561 gctcgccggc ggcgccggac gcggcgaggc ggccgtccgc gaggccctgg cggcgagccg
     1621 gggatccgtc ttcacggtat tcgagcagga gctgccgctg acgtgagcac gagcaggccc
     1681 tcctcgcggc ggtcgtggga cgccacggcc aggccggcgt cgcgtgcctc ggcctcgatc
     1741 tcctccgcga ggaaggcgtg ctcgtaccgg gcgctggcga tcccctgctc gaaggcgacc
     1801 acgtcgccgg gctcgggacg ccagtcggag cggctgagcc acgacgtggc gcgggcgacc
     1861 cgtggaccga tcgcccccca gggctccgac cgccggatgt agcagatcag cacgcgcccg
     1921 ccctcggcga ggtgcgagcg catgcgtcgc agcgtctcca cgcgcgcccg ccgcccggga
     1981 atgtaggaat agcagtacca ggagaagatc accgcgtcga acacgccggt gagctccgcc
     2041 gtctcgatgg cgccgacgtg cagcggcgtc cggtagcccc ggcgcttcag gttgagctgc
     2101 gccgcctcca cgcacgcggc cacgacgtcg agcccctcgg cgctgtagcc gcgctccagc
     2161 agcccgagga gatcgcggcc ggtgccgcat ccggcgacga ggatgcggtc cccccgctcg
     2221 aggaaccgcc cgtagaattc ctcctcccac gccatgagcg tgaacgtcgc atcggcggcc
     2281 tgcagctcgg cgaagcgccg ccacgccgcc tcccgcccgc gctcgaggtc ggcgcggcgc
     2341 acgagaccga cggcggcgtg gtgcgggagg cgtgacgcgt gctccagcgc cgtgcgcaca
     2401 ccgtcgagcg cggcgaagag cgcgcgacgg gccacgctca ccggctcagc agccgagggc
     2461 ggccccatcg gcgcgaggat cggagccgcc ggcgagccca tcggcgtcca cgcggatcac
     2521 gtgcgcgtgt cccatcaagt cgctccaggc ctcgaccacg cggacgtcgt gaccgcgtcg
     2581 acgcaggcgc tcggcgaccc cctcgccgaa gcggtcctcg atcctgagcg ccttcgacgg
     2641 ctccccccac gtgcgcccgt agagccagcg tggcgcctcg acggccgcct gcgggccgag
     2701 gccgcgatcg acgatgcggg tgacgagcgc cgcctgcgtc tggggctgac cctcgccacc
     2761 catcgtgccg tacaccaggc ggggccggcc gccgacgagg tacatcgacg ggatcagcgt
     2821 gtgggccgtc cgcttgcgtg gcccgagcgc gttcggatgc gccgggtcga gcgagaagaa
     2881 ggcaccgcgg ttctgcagca gcacgccggt gtcgccggcc accaccgccg cgccgaactc
     2941 gtggtacgtg gactggatca ccgacacggc gttgcccttg ccgtcggcgg tcacgatggc
     3001 gatcgtgtcg ccctcggccg ggacgcccgc cggcgccacc gcggcgtggg cggcgatccg
     3061 cgcgcgccgc cgcgccagcc gcgcggggtc caggcacgtg gtcacgggca ccgccacgac
     3121 gtcgggatcg gcgagccatc ggtcacggtc ctcgaaggcg agcttcgtcg cctcgacgat
     3181 caggtgcacg tgctcggcat cgtccaggcg tgccacgtcg aagccctcga gcagcgcgag
     3241 gatcgcgagc gccgcgaacc cctgcgtcgg cggcgggaag ctcgtggcct ccccgccgcg
     3301 atagggcacc cgcagcggct ccacccagtc cgccgtgtgc tcggcgaggt cgcgcgccgt
     3361 gagcgggctg ccggcggccg cggcgcccgc cgcgatgcgg cgggcgaggc cgccgcggta
     3421 gaactcctcg gcaccgccct cggcgaccgc ggccagcgtg gcggccagcg ccggctgcgt
     3481 gaagcggccc tcggcgaacc ggtccgggtg gtagatcggc cagaaggaac gccgtacctc
     3541 cgccggcgcg tcgcccgcga agagagccgc cgcctgcgcc gtcacccgct gctggcccgg
     3601 ggagacgggg aaaccctcgc gcgcgtggac gagcgcgtgc tggaaaagat ccggccaggc
     3661 gagcgacgag ccgagcgtct cgcggctcag gcggtgggcc tcccaccatc cggacacgac
     3721 accggggacc gtgagcgcgg cggcgccgcc ccgcgtcggc atcgtctcgc cgtgtctcgc
     3781 gcggtaggcg tcggcgtcca cggcggccgc cgagcgaccg gcggcgttca gggccaggag
     3841 ccgccgccgg cgcgcgtcgt ggatcagcca gaagttgtcg ccgccgatcg agttcatgtg
     3901 cgggtagacg acggcgatgg tggacgccgc cgcgacggcg gcgtccacgg cgttgccgcc
     3961 gcggcgcagc accgcgacac ccgcctcgga ggcgagcaga tggggagacg cgacgaggcc
     4021 gcgtgtcccg tgcgcttcgc tccagcgcgt ggtcacgcgc gccatcatac agcctcggcc
     4081 gaccgcaccg acctcgagga ccgggacgtc accttccgtc gcatcggcga cgagatcgtg
     4141 ccgctcgtgc tgctgggctc accccgcgcc gaggcaccgg ctctccgacg cgtctcgccc
     4201 ccgcgccgga gtgagggcgc tttgcggtac gatgcagcgg ggccatgttg acgaccacga
     4261 tcgcgggcag cctgcccaag ccgcggtggc tcgccgcccc cgagacgttg tgggcgccct
     4321 ggcggctccc ggacgacacg ctccgcgagg gtcagcgcga tgccgtggtc gtcgccctga
     4381 agctccagga gacggccggt atcgacgtgg tcaccgacgg cgagcagtcg cggcagcact
     4441 tcgtgcacgg cttcgtggcg gggctcgcgg ggatcgactt cggcaagcgg gtgacgatcg
     4501 gcatccgcgc cgaccgctac aaggccgagg tgccgacggt cacgggaccg atcgcccgcc
     4561 gggcgcccgt ccacctggag gacgtccggt gggcgcgcgc ccacaccgaa cgcaggctca
     4621 agttcaccat ccccggcccg atgacgatcg tggacacgct ggccgacgag cactaccgcg
     4681 accgcgcgcg gctcgccctc gcattcgcga aggtgatcaa cgaggaggcg cgcgacctcg
     4741 tgggcgccgg cctggacgtg ctgcagctcg acgagcccgc cttcaacgtc tacatggacg
     4801 acgtcgcggc gtggggcatc gaggcgctgc accgggccac cgaggggctc ggctgcacga
     4861 ccgccgtgca catctgctac ggctacggca tcaaggcgaa cgtggactgg aagcgcacgc
     4921 tcggcagcga gtggcggcag tacgagaaga ccttcccgct gctggcgcag agccggatcg
     4981 accaggtttc gctcgagtgc gcgaactcgc gggtcccgct cgagctgctc ggtctgctgc
     5041 ggggcaagga cgtcctcgtg ggcgccgtgg acgtcgccag cctcgacgtc gagtcgccgg
     5101 agcaggtggc cgccaccatt cgccgcgcca tggcgttcgt gccgcccgag cggctctggc
     5161 cgtgcacgaa ctgcggcatg gtgccgctgc cgcgcgcggt ggcggaaggc aagctccgcg
     5221 cgctcggcgc cggggcgcgc ctcgtgcggg ccgagctcgg cgcgcgttga tccgcgtcgc
     5281 ggtgagccgc accgccgcgc ccgcctccgt gctcgtcgtg gtcctcgccg ccccgatggc
     5341 ggtcgcgctc cacggggtgc cggcctgcgc cgccgcgctc gcgcgccctg ggaagcgccc
     5401 gcccggccct gcgcgctgat gcgctggctc gtcgacggct acaacgtcat ccggcgcgat
     5461 cccgacctgc gtggcgcgga agcagccggt ctcgaggcag gccgggccgc gctcctgcgc
     5521 gcgatcgcgc ccgtcgcacg gcgctcggcc gaccgcttca ccgtcgtctt cgacggcgcg
     5581 ccttcgcggg cgccctcccc ggctccgggc cggctcgagg tggtcttctc ccgaccgccc
     5641 cagaaggccg acgacgtgct gggacgcatg gcccgcgagg cgggcgccgg caccgtcgtc
     5701 gtcagcgacg accgcgcggt ggccgacgcc
//
LOCUS       DATIAZ010000352         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_506190, whole genome shotgun sequence.
ACCESSION   DATIAZ010000352 DATIAZ010000000
VERSION     DATIAZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506190"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VLF19_12030"
     CDS             <1..1214
                     /locus_tag="VLF19_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSE94027.1"
                     /translation="TTAKLSLVDGGEPLTAYAFEAARQKRFLEGSGLPIRISTIELIE
                     IGAGGGSIAHVDEIGLLKVGPRSAGSQPGPAAYGLGGIEPTVTDADFALGYLDPGYFA
                     GGEMRIDMASARAALDRLAARVALPLVECAWGVHDVVNENMAGAARVHIAERGRDPRR
                     YALLCTGGAGPVHAYYVARKLGLSQVVCPPAAGVASALGLLMAPARVDRVATVGIRLD
                     RDSVAALEKAFRRLEDDARAVMADSGLKLDAATVRRFADGRFLGQGFDLVVDLPDGPY
                     DDSDACRQRLTAAFETAYREKFALTPPEVPVEFINVRVAVRAPVAGAEVTVRGEAAGG
                     DGAVKGVRPVYFPERRRFEQTTVYDRARLAPGDEIRGPAVVEEEGSTLVIGPGGTARV
                     AATGNIVVTLP"
     gene            1211..>2559
                     /locus_tag="VLF19_12035"
     CDS             1211..>2559
                     /locus_tag="VLF19_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSE94028.1"
                     /translation="MTTRLDAVTLEVLWTRIISVVDEAAKAIVRTSFSTLSNEANDFA
                     CMLTDARGYALAQNSGSIPSFIGTLPATVRHFLREMGAERMQPGDVLITNDAWMGTGH
                     MSDVSVLAPIFHRGRLVAFSATTSHMPDIGGRIRAIEAREIFEEGLHIPLTKLVHAGR
                     VDDTLVALVRANVRTPDQTMGDIWAQVSANELMGRRVIALMDDYGLDGLTALGDELFA
                     RSEGAMREAIRAVPDGTYGYGFRTDGTGTPFEFRIALTIAGDEIVADYTGTSPQQPRA
                     INCVMAYTYAMTAYAVRCALLPGLPNNEGMYRPIKVVAPEGSLLNPRFPAAVVSRATT
                     GHYVPALVLGALHRVIPERVMAGAGSPLWALSQSSTREDGKPYTTVLFFNGGMGATPV
                     KDGESVLSWPSNISSTPVEVAERNSPLFFHYKRLRPGSGGRGRFRGGLGQDILIESRS
                     "
BASE COUNT          352 a          943 c          902 g          362 t
ORIGIN      
        1 gcaccaccgc caagctcagc ctggtggacg gcggcgaacc gctcaccgcg tacgcgttcg
       61 aggcggcgcg ccagaagcgc ttcctggagg gcagcgggct gccgatccgc atctccacca
      121 tcgagctgat cgagatcggc gccggcggcg gctccatcgc tcacgtggac gagatcgggc
      181 tgctcaaggt cggcccccgc agcgcgggct ctcagcccgg tcccgccgcg tacgggctgg
      241 gcgggatcga gccgaccgtg accgacgccg acttcgccct gggctacctc gatcccggct
      301 acttcgccgg cggcgagatg cgcatcgaca tggcctcggc tcgggcggcc ctcgaccgtc
      361 tggccgcgcg cgtcgcgctc ccgctcgtcg agtgcgcgtg gggcgtccac gacgtggtca
      421 acgagaacat ggccggggcc gcccgcgtgc acatcgccga gcgcggccgc gacccccgcc
      481 gctacgcgct gctctgcacc ggcggggccg ggccggtcca cgcctactac gtcgccagga
      541 agctcggcct gagccaggtc gtctgtcctc cggccgccgg ggtggcctcc gcgctgggtc
      601 tgctgatggc accggcgcgg gtggaccggg tggccacggt cggcatccgg ctggatcgcg
      661 acagcgtcgc cgcgctcgag aaggcgttcc ggcggctgga ggacgacgcg cgggcggtga
      721 tggccgactc cgggctgaag ctcgacgccg ccaccgtgcg ccgtttcgcc gacggccggt
      781 tcctcggtca gggcttcgac ctcgtcgtcg atcttcccga tggtccctac gacgactcgg
      841 acgcgtgccg ccagcggctg accgccgcgt tcgagaccgc ctaccgcgag aagttcgcgc
      901 tcacgccgcc ggaggtgccg gtggagttca tcaacgtccg ggtggccgtt cgagctccgg
      961 tggcgggcgc ggaggtgacg gtgcgcggtg aggccgccgg tggtgacggc gcggtcaagg
     1021 gcgtgcgccc ggtttacttc cccgagcggc gccggttcga gcagacgacc gtgtacgacc
     1081 gcgcgcggct ggcgcccggc gacgagatcc gcggtccggc ggtggtcgag gaggaaggct
     1141 cgacgctggt gatcggcccc ggcggtaccg cccgggtggc ggcgaccggc aacatcgtgg
     1201 tgacgctgcc atgaccacca ggctcgacgc cgtcacgctc gaggtgctct ggacgcgcat
     1261 catctcggtg gtggacgagg cggccaaggc catcgtccgc acgtcgttct ccaccctgtc
     1321 caacgaggcc aacgacttcg cctgcatgct gaccgacgcg cgcgggtacg cgctggccca
     1381 gaacagcggc agcatcccct ccttcatcgg cacgctgccc gccaccgtcc gtcacttcct
     1441 cagggagatg ggcgccgagc ggatgcagcc gggcgacgtg ctcatcacca acgacgcctg
     1501 gatgggcacc ggccacatga gcgacgtatc ggtgctcgcg ccgatcttcc accgcggccg
     1561 gctggtcgcg ttctcggcca ccacctcgca catgccggac atcggcggcc gcatccgcgc
     1621 catcgaggcc cgcgagatct tcgaggaagg cctgcacatc ccgctgacga agctggtcca
     1681 cgctggccgc gtggacgaca cgctcgtcgc gctcgtgcgc gccaacgtgc ggacgccgga
     1741 ccagacgatg ggcgacatct gggcccaggt gagcgccaac gaactgatgg ggcgccgggt
     1801 gatcgcgctc atggacgatt acggcctcga tgggctcacc gcgctgggcg acgagctgtt
     1861 cgcgcgctcc gagggggcga tgcgcgaagc catccgcgcg gtgccggacg gcacgtacgg
     1921 ttacggcttc cgcaccgacg gcaccggcac gcccttcgag ttcaggatcg cgctcacgat
     1981 cgccggcgac gagatcgtgg ccgactacac gggcacctcg ccccagcagc cgcgcgccat
     2041 caactgcgtg atggcttaca cctacgcgat gacagcctac gccgtgcgct gcgcgctgct
     2101 gcccgggctg cccaacaacg agggcatgta ccgcccgatc aaggtcgtgg cgcccgaggg
     2161 ctccctgctc aacccacgct tcccggccgc ggtggtgagc cgcgccacca ccggccacta
     2221 cgtgcccgcg ctggtcctgg gcgcgctcca ccgcgtgatc ccggagcgcg tcatggccgg
     2281 cgccggctcg ccgctgtggg cgctgagcca gtcgagcacg cgggaggacg gcaagcccta
     2341 caccacggtg ctcttcttca acggcggcat gggcgccacg ccggtcaagg acggcgagag
     2401 cgtgctctcg tggccgagca acatctcctc cacccccgtg gaggtggccg agcgcaacag
     2461 cccgctcttc ttccactaca agcgtctgcg gccgggctcg ggcggccgcg gccgcttccg
     2521 cggcggcctc ggccaggaca tcctcatcga gagccggtc
//
LOCUS       DATIAZ010000353         5785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1821660, whole genome shotgun sequence.
ACCESSION   DATIAZ010000353 DATIAZ010000000
VERSION     DATIAZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821660"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2716
                     /locus_tag="VLF19_12040"
     CDS             <1..2716
                     /locus_tag="VLF19_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSE94029.1"
                     /translation="LDVPIATPAERAFVEALTAASPAVLATVPDGDDATLDALRSLGA
                     RERRDEPPARDEPGGDLARARDFLFAAAVPGAESPRGDVVFFSAPGEGRETVEIARWI
                     LDEARAGTRFDEMAVLLRARHLYVSLLEVALRRAGIPAWFARGTSRPDPSGRAFLALL
                     DCALEGLSARRFAEYLSLGQVPSLDAAGAPPLDRLVWTAPEEEILGPAAEATPAVSPE
                     AAPSDTSGSPEPDADDHPVVEGTLRAPWRWEQLLVESAVIGGQARWSRRLAGLEAEYR
                     ARLARYREEEPDSPRTSAIERDLRNLEHLRRFAVPVIERLAALPARGTWGEWIAALET
                     LAPIVLRRPERVLTVLAALRPLEVIGPVALGEVRDVLAGELTTLAERPPADRYGRVFV
                     GTIEQARGRSFEVVFLPGLSERIFPQKPREDPILLDALRRELGASLATQDDRGRRERL
                     LLRLAVGAAGRRLHLSYSRIEQTEARPRVPSFYALEVERALTGRVPEPQRMEREAAAA
                     AGARLAWPAPDDPTRAIDEVEHDLASLRALLASGQARGRARYLLELNPALARSLRTRW
                     SRWNRSFAPADGLVRVADGTRETLSTARLAARAYSASALQKFAACPYQFFLSAICRLE
                     PRPEVATIVELDPATRGHLFHRVQADAMRALADEGRLPLTPGALQPAQATLDRTLDRV
                     AEQYREDLAPAIRRVWQDEVESMRADLRMWLEQSAATQAAWEPIAFELAFGLRRDPAN
                     DPRSVESEVTLPEGYRLRGIVDLVERRRGGPELRVTDYKTGRDYARRNRLVVGGGEML
                     QPVIYGLAVEHLLAARVVESRLYYCTRAGGFSERLVPLTGETRGRGAEVLGVIDRAIA
                     AGFLPAAPRKGACGICDFRDVCGPREEVRITLKDQARIEELRALRDSP"
     gene            2713..>5785
                     /locus_tag="VLF19_12045"
     CDS             2713..>5785
                     /locus_tag="VLF19_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSE94030.1"
                     /translation="MTRLVDQAERERIRTSLDETLVVEAAAGTGKTSELVARLVNVLA
                     EGRGSVQTIAALTFTEKAAGELKLRLRAGLEDERAGAAEGSARRARLEEAIAHLEEAR
                     VSTIHGFCNDILHERPVEARVDPGFEVLPESEAEALYRRAFDGWLAETLEAPPEGLRR
                     ALRRRSFDGDPVDRLRKAGWQLVGWRDFRAPWQRRAFDREAAIDALVARVHGLNARLT
                     TCSTTADTLYADLWPLRRLSEDVGTRERVAPRDHDGLEAALVDLLRERSFKGPRRGQT
                     RNYRGRVTRDDVLAAHGDLLAALEVFAREADADLAALVQQALLETVDRYEELKTRVAA
                     LDFVDLLLRARDLVRDHGGVRADLQRRLTHIFVDEFQDTDPLQAEIVLLLAASDPGVS
                     RWQDIAPAPGKLFLVGDPKQSIYRFRRADVATYQAVKELLVASGAACVYLTTSFRAIP
                     SIQHLVNAAFAPVMTDDPAALQAGYVPLARHRDDIPGQPGVVALPVPKPYGHWGGFAK
                     AAVEESLPDAVGAFVAWLLSESGWKVTERERPGEALPISARHVCLLFRRFTSWGEDVT
                     RRYVDALEARGVPHLLVGGRSFHLREEVESLRTALAAVEWPDDELSVYATLKGPLFAI
                     GDEELVEYRQRFRRLHPYRLPREAVGAHLKPVVEALELLRSLHGLRNYRLVEETVNRL
                     LTATRAHAAFVLRPWGEQALANVLRVGELARTYEAAGSISFRGFVERLSEEAEGEAPE
                     APIVEESSEGVRIMTVHKAKGLEFPVVILADITAGIAAWNPGRWVDAGRGLCALRLGG
                     WPPWDLLDHEADELARDRAEGVRIAYVAATRARDLLVVPAIGDDPFQAGWDGASDGWI
                     GPVHTAVYPRAERRRAPQAAPGCPAFGEDSVLERRDRDTPGPDNVRPGLHAFGDYEVT
                     WWDPRMLALDVQRVYGLRREDLILDPGRETVEADRKRYDGWLAARREAQELGARPSLR
                     VSVITEWARAKPEGDEIGRDVVLADGAAGGPRPGGPRFGTLVHAALATVALD"
BASE COUNT          710 a         2041 c         2280 g          754 t
ORIGIN      
        1 gctggacgtg ccgatcgcca cgccggccga gcgcgctttc gtcgaagcgc tcacggccgc
       61 gtcgccggcg gtgctcgcga cggtgcccga cggtgacgac gccacgctcg atgcgctgcg
      121 gagcctgggg gcgcgcgagc ggcgcgacga gccgcccgca cgcgacgagc cgggcggcga
      181 cctcgcacga gcgcgggact tcctgttcgc ggcggccgtc ccaggggcgg agtcgccgcg
      241 tggggacgtc gtcttcttct cggcccccgg cgagggacgg gagacggtcg agattgctcg
      301 gtggatcctc gacgaagcgc gggcggggac gcggttcgac gagatggcgg tgctgctgcg
      361 cgcgcgtcac ctgtacgtgt cgctgctcga ggtggcgctg cggcgggcgg gcatcccggc
      421 ctggttcgcg cgagggacgt cgcggcccga cccgtccggc cgcgcgtttc tcgcgctgct
      481 cgactgcgcg ctcgagggac tctcggcgcg gaggtttgcg gaatacctct cgctcggcca
      541 ggtgccgtcg ctcgacgcgg ccggcgcgcc gccgctggat cgcctggtgt ggacagcccc
      601 cgaggaagag attcttggcc cagcggcgga agccacgccg gcggtgagcc cggaggcggc
      661 gccgtccgac accagcggct cgccggaacc cgacgccgac gaccaccccg tggtcgaggg
      721 caccctgcgt gcgccgtgga gatgggagca gctcctggtc gagtccgccg tgatcggcgg
      781 tcaggcccgc tggagtcgcc gcctggcggg gctcgaggcg gaataccgcg cgcgactcgc
      841 ccggtatcgc gaggaggagc cggactcgcc acggacctcg gcgatcgagc gcgatctccg
      901 caacctcgag cacctccgtc gcttcgcggt cccggtcatc gagcggctcg ccgcgctccc
      961 cgcgcgcggc acgtggggtg aatggatcgc ggcgctcgag acgctcgcgc cgatcgtgct
     1021 gcgacggccg gagcgggtgc tgaccgtgct ggccgcgctg aggccgctcg aggtgatcgg
     1081 gcccgtcgcg ctcggcgagg tgcgcgatgt cctggccggt gagctgacga cgctcgccga
     1141 gcgtccaccg gccgaccggt acgggcgcgt cttcgtcggt acgatcgagc aggcccgggg
     1201 gcgaagcttc gaggtggttt tcctgccggg attgtccgag cggatcttcc cccagaagcc
     1261 ccgcgaggac ccgatcctcc tggacgcgct ccggcgcgag ctcggggcct ctcttgccac
     1321 gcaggacgac cggggtcgcc gcgagcgcct cctcctccgg ctcgccgtcg gcgccgcggg
     1381 ccggcggctg catctctcgt actcacgcat cgagcagacg gaggcgcgcc cacgggtgcc
     1441 gtccttctac gcgctcgagg tagagcgcgc gctcacgggc cgggtgccgg aaccccagcg
     1501 gatggagcgc gaggcggcgg ccgccgcggg ggcgcgcctg gcgtggccgg cgccggacga
     1561 cccgacacgc gcgatcgacg aggtggagca cgatctggcg agcctgcgcg cgctgctcgc
     1621 gagcggccag gcgcgcggcc gcgcccgcta cctgctcgag ctgaacccgg cactcgcgcg
     1681 ctcgctgcgc actcggtggt cgcgctggaa ccggagcttc gcgccggcgg acggcctggt
     1741 ccgggtggcg gacggcacgc gggagacact gtcgacggcg cggctggcgg cgcgcgcgta
     1801 ctcggcctcg gcgctccaga agttcgcggc gtgcccgtat cagttctttc tctccgcgat
     1861 ctgccggctg gagccgcgcc cggaggtggc aacgatcgtg gagctggatc cggccacacg
     1921 cgggcatctc ttccaccgcg tgcaggcgga cgcgatgcgc gcgctggccg acgagggacg
     1981 gctgccgctt acacctgggg cgctgcagcc ggcgcaggcg acgctcgacc ggacgctcga
     2041 tcgcgtcgcc gagcagtacc gcgaggacct ggcgccggcg atccggcgcg tctggcagga
     2101 cgaggtcgag tcgatgcgcg cggacctccg catgtggctc gagcagtcgg ccgcgacgca
     2161 ggccgcgtgg gagccgatcg ccttcgagct ggcgttcggg ctccggcgcg acccggcgaa
     2221 cgacccgcgc agcgtcgagt cggaagtcac gctgcccgag ggctaccggc tgcgcggcat
     2281 cgtggacctg gtggagcgcc ggcgcggcgg gccggagctg cgcgtgaccg actacaagac
     2341 gggccgcgac tacgcgcggc gcaatcgcct cgtcgtgggc ggcggcgaga tgctccagcc
     2401 ggtcatctac ggcctggcgg tcgagcacct gctggccgcg cgggtggtgg agtcgcggct
     2461 gtactactgc acgcgcgccg gcgggttctc ggagcgcctg gtgccgctca ccggcgagac
     2521 gcggggccgc ggcgcggagg tgctcggcgt gatcgaccgt gccatcgccg ccggcttcct
     2581 gccggccgcg ccgcggaagg gggcgtgcgg gatctgcgac ttccgcgacg tctgcggccc
     2641 gcgcgaggag gtccgcatca cgctcaagga ccaggcgcgg atcgaggagc tgcgcgcgct
     2701 gcgggactcg ccgtgacgcg gctcgtcgac caggccgagc gggagcgcat ccgcacctcg
     2761 ctcgacgaga cgctcgtggt cgaggctgcc gccggtaccg gcaagaccag cgagctggtc
     2821 gcgcgcctcg tcaacgtgct ggccgagggc cgcgggagcg tgcagacgat cgccgcgctc
     2881 acgttcaccg agaaggcggc gggggagctg aagctgcggc tgcgcgccgg gctcgaggac
     2941 gagcgtgcgg gcgcggcgga ggggagcgcg cgacgcgcgc ggctcgagga ggccatcgcc
     3001 cacctcgagg aggcgcgcgt cagcaccatc cacggcttct gcaacgacat cctgcacgag
     3061 cggcccgtcg aggcgcgcgt ggacccgggc ttcgaggtgc tcccggagtc ggaggccgag
     3121 gcgctctacc ggcgcgcctt cgacggctgg ctggccgaga cgctcgaggc gccgccggag
     3181 ggactgcggc gcgcgctgcg ccggcgctcg ttcgacgggg atcccgtcga ccgcctgcgg
     3241 aaggcgggct ggcagctcgt cggctggcgc gacttccgcg cgccgtggca gcggcgcgcg
     3301 ttcgaccggg aggcggccat cgacgcgctc gtcgcgcggg tgcacgggct gaacgcgcgg
     3361 ctcacgacgt gctcgaccac ggccgacacg ctctacgcgg acctctggcc gctcaggcgg
     3421 ctgagcgagg acgtcggcac gcgcgagcgc gtggcgccac gcgaccacga cgggctcgag
     3481 gcggcgctgg tcgacctgct tcgcgagcgc agcttcaagg gcccgcgccg cggccagacg
     3541 cgcaactacc gcggccgcgt aacccgggac gacgtgctcg ccgcgcacgg ggacctgctg
     3601 gccgcgctgg aggtcttcgc gcgggaggcg gatgcggacc tggcggcgct cgtccagcag
     3661 gcgctgctcg agacggtcga ccgctacgag gagctgaaga ctcgcgtcgc cgcgctcgac
     3721 ttcgtggatc tcctcctgcg cgcgcgcgac ctcgtgcgcg accacggcgg ggtgcgcgcg
     3781 gacctccagc ggcggctcac gcacatcttc gtcgacgagt tccaggacac cgacccgctg
     3841 caggcggaga tcgtgctgct gctggcggcc tccgaccccg gggtcagccg ctggcaggac
     3901 atcgcgccgg caccgggcaa gctcttcctg gtaggcgacc ccaagcagtc gatctaccgc
     3961 ttccggcgcg ccgacgtcgc cacctaccag gcggtgaagg agctgctggt ggcgagcggc
     4021 gcggcgtgcg tctacctgac gacgagcttc cgcgcgatcc cgtccatcca gcacctcgtg
     4081 aacgcggcgt tcgcgccggt gatgacggac gaccccgcgg cgctccaggc gggctacgtg
     4141 ccgctggccc gccaccgcga cgacatcccg gggcagccgg gcgtcgtcgc gctgccggtg
     4201 cccaagccct acggtcactg gggcgggttc gcgaaggcgg cggtggagga gtcgctgccc
     4261 gatgccgtcg gcgcgttcgt ggcgtggctg ctctcggaga gcggctggaa ggtgaccgag
     4321 cgcgagcggc ccggcgaggc gctgcccatc tccgcgcgcc acgtgtgcct gctgttccgg
     4381 cgcttcacgt cgtggggtga ggacgtcacg cggcgctacg tcgacgcgct cgaggcgcgc
     4441 ggggtccccc atctcctggt cggaggccgg tcgttccacc tgcgcgagga ggtggagagc
     4501 ctgcgcacgg cgctggcggc cgtcgagtgg ccggacgacg agctgagcgt gtacgcgacg
     4561 ctcaagggcc cgctcttcgc catcggcgac gaggagctgg tggagtaccg ccagcgcttc
     4621 cggcgcctgc atccctaccg gctcccgcgc gaggccgtcg gggcgcatct gaagccggtg
     4681 gtcgaggcgc tcgagctcct gcgctcgctc cacgggctcc gcaactaccg gctggtcgag
     4741 gagacggtga accggctgct cacggccacg cgcgcgcacg cggccttcgt gctgcgcccg
     4801 tggggcgagc aggcgctggc caacgtgctg cgcgtgggcg agctggcgcg cacgtacgag
     4861 gcggcgggga gcatctcgtt ccgcggcttc gtcgagcggc tcagcgagga ggcggagggc
     4921 gaggcgccgg aggcgcccat cgtggaggaa tcgagcgagg gcgtgcgcat catgaccgtc
     4981 cacaaggcca aggggctcga gttcccggtg gtgatcctgg ccgacatcac ggccggcatc
     5041 gccgcctgga accctggccg ctgggtcgac gccggacgcg ggctctgcgc gctgcgcctg
     5101 ggcggctggc caccgtggga tctgctcgac cacgaggcgg acgagctggc gcgcgaccgc
     5161 gcggaggggg tgcgcatcgc ctacgtggcg gccacgaggg cgcgcgacct gctggtggtc
     5221 ccggcgatcg gcgacgatcc cttccaggcc ggctgggacg gtgcgagcga cgggtggatc
     5281 ggtccggtcc acaccgccgt ctatccgcgg gcggagcggc ggcgcgcgcc gcaggcggcg
     5341 ccgggctgcc cggcgtttgg cgaggacagc gtgctggagc ggcgcgaccg cgacacgccc
     5401 gggcccgaca acgtccggcc gggcctgcac gcgtttggcg attacgaagt cacgtggtgg
     5461 gacccccgga tgctggcgct cgacgtgcag cgcgtctacg ggctcaggcg tgaggatctg
     5521 atcctggacc ccggccgcga gaccgtcgag gccgatcgca agcgatacga cggctggctg
     5581 gccgcgcgcc gggaggcgca ggagctgggg gcgcggccca gtctgcgcgt cagcgtgatc
     5641 accgagtggg cacgggcgaa gccggagggc gatgagatcg gccgcgacgt cgtgcttgcc
     5701 gacggggccg cgggcgggcc gagaccgggc ggtccgcgct tcggaacgct ggtgcatgcg
     5761 gccctggcca ccgtcgcgct cgatg
//
LOCUS       DATIAZ010000354         5485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_769066, whole genome shotgun sequence.
ACCESSION   DATIAZ010000354 DATIAZ010000000
VERSION     DATIAZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769066"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VLF19_12050"
     CDS             complement(<1..251)
                     /locus_tag="VLF19_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94031.1"
                     /translation="MTAPTGGGAFPLAAVRAAGEATLHCALLATALAWTAWDDGAASQ
                     AAWDPQPAILSLAVLVTLAAVLTEIDPRAGVAWLAPGLV"
     gene            complement(334..1023)
                     /locus_tag="VLF19_12055"
     CDS             complement(334..1023)
                     /locus_tag="VLF19_12055"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HSE94032.1"
                     /translation="MGASIAPSSGDDLAVAYRRLNAAYAQTLRYAEDVRHLYQRTQRA
                     ISQSLLGLANALEAKDPYTRGHSERVGDWSRRLAGMLELSPAEVESVAQAGLLHDIGK
                     IGVPEAVLGKRGPLDPDEWAYMRRHPLVGAQIVAPFEFLAPGALVIRHHHERWDGSGY
                     PDGLAGPRIPLGARIVAVADVYDALTSTRPYRAALSHPAAIDHLLVEAGRTLDAHVVA
                     AFVGLTRDVGP"
     gene            complement(1008..1394)
                     /locus_tag="VLF19_12060"
     CDS             complement(1008..1394)
                     /locus_tag="VLF19_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE94033.1"
                     /translation="MPTVLIVDDEPNIVELVRLTLEDERVRIVDAADGAEALVRAEAV
                     KPDLVLLDIGLPDMSGLDVCRRLRHDPRFAPARIVMLTAMAQRDDVASGLAAGADHYL
                     TKPFSPVRLLTLVSELVPGAASWARP"
     gene            complement(1690..2112)
                     /locus_tag="VLF19_12065"
     CDS             complement(1690..2112)
                     /locus_tag="VLF19_12065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HSE94034.1"
                     /translation="MADTLTLEVATPTRLVLSELVDEVVAPGIEGYFGVLPGHAPFLT
                     TLGVGELTYRKGHDEFHLAIAGGFAEVRNDKVIVLADSGERPDEIDRARAERARERAE
                     ARLAGRSPHEPLDAIDYIRALAALARALTRLFVAGRGQ"
     gene            complement(2121..3542)
                     /gene="atpD"
                     /locus_tag="VLF19_12070"
     CDS             complement(2121..3542)
                     /gene="atpD"
                     /locus_tag="VLF19_12070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSE94035.1"
                     /translation="MSNGKIVQVIGPVVDVEFEPGRLPAIYNALVVEGVENKDIFSYS
                     QKLTLEVAQHIGESQVRTVAMAATDGLTRGMVVADTGAPISVPVGKETLGRILNIVGE
                     PVDKGPALQVKKTYPIHRPAPAFEDQSTKVEMFETGIKVVDLLEPYTKGGKTGLFGGA
                     GVGKTVLIQELINNIAKQHGGISVFAGVGERTREGNDLYHEMKESGVLEKTALIFGQM
                     TEPPGSRLRVALTALTAAEYFRDEEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVG
                     YQPTLGTEMGELQERITSTKKGSVTSVQAIYVPADDITDPAPATAFAHLDATTVLSRP
                     LTELGIYPAVDPLASTSRILDPNILGDDHYQTARAVQSILQRYRDLQDIIAILGMEEL
                     SDEDKMIVARARKIQRFLSQPMFVAEAFTGTPGRYVKLADTVKSFKEVVEGKHDELPE
                     QAFYMVGDIGDAMEKAKRLRELA"
     gene            complement(3539..4429)
                     /gene="atpG"
                     /locus_tag="VLF19_12075"
     CDS             complement(3539..4429)
                     /gene="atpG"
                     /locus_tag="VLF19_12075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HSE94036.1"
                     /translation="MATPRDIRRRIRSVQSTQKITRAMKLVAAAKLRRAQERILAARP
                     YAGKMAELLGNLVGVTGSEGAPHPLLEQREGPRRQIVVITADRGLAGAFNSNILRKAM
                     EAVRESTSPTDLTLVVVGRKARDFYRRRQWTIKRDLIGFWDRLAYSHAQELADYFMQQ
                     YVDGEVDEVHLIYNEFRSVAVQRPVRQQLLPIPRVHAAGGDGAETPVDYIFEPGPAAI
                     LGDLLPRHVRMQVFRALMESLAGEYGARMTAMEAATKNAKEMIDVLTIQYNKARQEKI
                     TKELLDIVGGAEALTQGAEA"
     gene            complement(4436..>5485)
                     /gene="atpA"
                     /locus_tag="VLF19_12080"
     CDS             complement(4436..>5485)
                     /gene="atpA"
                     /locus_tag="VLF19_12080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSE94037.1"
                     /translation="RGQRELIIGDRGTGKTAIAVDTIINQKGQDVFCFYVAIGQKRST
                     VAQVVKVFEDAGAMAYTTVVVASASEPAPLQYIAPYAGVTMGEYFRDSKRHALCVYDD
                     LSKHATAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDELGGGSLTALPIIE
                     TQLGDVSAYIPTNVISITDGQIYLEADLFYSGIRPAVNVGLSVSRVGGSAQIKAMRQV
                     AGKLRLDLAQFRELAAFAQFGSDLDRATQMQLARGQRMVEVLKQNQYDPLPVEKQVAI
                     IFAGTQGLLDDLPVDAVRAFEQHLAGWLERRQSRVLAEIRDKKEISDAAREQLTTAVQ
                     EAKTEFVAARSVKAA"
BASE COUNT          757 a         1894 c         1995 g          839 t
ORIGIN      
        1 tagacgaggc ctggcgcgag ccacgcgacc ccagcccgcg ggtcgatctc ggtgagcact
       61 gcagccagcg tgacgagcac ggcgagagag aggatcgccg gctgcgggtc ccacgccgcc
      121 tgcgaggcgg ccccgtcgtc ccacgccgtc cacgcgagcg ccgtggccag cagggcacag
      181 tgcagggtcg cctcgccggc ggcgcgcacg gcggcgaggg ggaacgcgcc gccgcccgtg
      241 ggcgcggtca cggccagagc gaggacggcg agtccggcga gcgggagcct gggggtgccc
      301 gcgagccagg accgcgcggg cggcgcggga tgctcaaggg cccacatcgc gcgtgaggcc
      361 gacgaaggcg gcgacgacgt gcgcgtccag cgtgcggcct gcctcaacga ggaggtggtc
      421 gatggccgcc gggtgcgaca gcgcggcgcg gtacggccgc gtcgacgtca gcgcatcgta
      481 gacgtcggcg acggcgacga tgcgggcgcc gagcgggatg cgagggccgg ccaggccgtc
      541 cggatagccg ctgccgtccc agcgttcgtg atgatgacgg atgacgagcg cgcccggggc
      601 gaggaactcg aacggcgcga cgatctgcgc gccgacgagc gggtgccgcc gcatgtaggc
      661 ccactcgtcc gggtcgagcg gcccgcgttt gccgagcacc gcctcgggca cgccgatctt
      721 gccgatgtcg tgcagcagtc ccgcctgcgc caccgactcc acctcggccg gagacagctc
      781 gagcatgccc gccagccggc ggctccagtc cccgacgcgc tcggagtgtc cgcgcgtgta
      841 gggatctttc gcctcgagcg cgttcgccag gccgagcagc gactgggaga tggcgcgctg
      901 cgtgcgctgg tagagatggc ggacgtcctc cgcgtagcgc agcgtctgcg cgtaggcggc
      961 gttgagccgc cggtaggcga cggcgagatc gtcgccggag gacggggcta tggacgcgcc
     1021 cacgaggcgg ctcccgggac cagctccgac acgagcgtga ggagccggac cggcgagaac
     1081 ggcttggtca gatagtggtc ggcgcccgcg gcaagcccgc tcgcgacgtc gtcccgctgc
     1141 gccatcgcag tcagcatgac gatgcgggcc ggcgcgaagc gcggatcgtg ccggagacgg
     1201 cgacagacat cgagccccga catgtcgggc aggccgatgt ccaggagcac gaggtccggc
     1261 ttgaccgcct cggcgcggac cagcgcctcg gcaccgtccg ccgcgtcgac gatgcgtacg
     1321 cgctcgtcct cgagcgtgag gcggacgagc tcgacgatgt tgggctcgtc gtcgacgatc
     1381 agtacggtcg gcacgactcg cgacggttgc gagagccgtg ccggccgggc gggttcacgt
     1441 cggcgcggac ttacgcgcgg gcctcgaacg cggttgctcg ccgcgcgctg catgttgccc
     1501 tgccctcagg gcagcgcgtg caggtgccgg ccggcgctcc ggcgacggcc tcggcgaagg
     1561 gcggcgtcac cgaggagcga cacgcgcggc ggacggcgcg gcgccggatc gacgggtcgg
     1621 atcggcaagg gccgcggcgc cgccgccggg tctcacccga tcggccgcgg gcgctgcggt
     1681 tccttgcggc tactgtcccc ggccggcgac gaacaggcgg gtcagcgcgc gcgccagagc
     1741 ggccagcgcg cggatgtagt cgatcgcgtc cagaggctcg tgcggcgagc ggccggcgag
     1801 ccgggcctcg gcgcgctccc gggcgcgctc ggcgcgggcg cgatcgatct cgtccggccg
     1861 ctcgccggag tccgccagca cgatcacctt gtcgttgcgg acctcggcga agccgccggc
     1921 gatcgccagg tgaaactcgt cgtgcccctt ccggtaggtc agctcgccga cgcccagcgt
     1981 ggtgaggaac ggggcgtggc ccggcagcac gccgaagtag ccctcgatgc cgggggcgac
     2041 cacctcgtcg accagctccg acagcacgag ccgcgtgggt gtcgcgactt cgagggtgag
     2101 cgtgtcggcc actggctcag ctaggccagc tcgcggagcc gcttggcctt ctccatcgca
     2161 tcgccgatgt cgccgaccat gtagaacgcc tgctcgggca gctcgtcgtg cttgccctcc
     2221 acgacctcct tgaagctctt cacggtgtcg gcgagcttca cgtaacggcc cggcgtgccc
     2281 gtgaaggcct cggccacgaa catcggctgc gagaggaagc gctggatctt gcgggcccgc
     2341 gccacgatca tcttgtcctc gtcgctgagc tcctccatgc cgaggatcgc gatgatgtct
     2401 tgcaggtcgc ggtagcgctg caggatggac tgcaccgccc gcgccgtctg gtagtggtcg
     2461 tcgcccagga tgttcgggtc gaggatgcgc gaggtcgagg ccagcggatc gacggcggga
     2521 tagattccca gctcggtgag cgggcggctc aggacggtcg tggcgtcgag gtgcgcgaac
     2581 gccgtcgccg gcgccgggtc cgtgatgtcg tcggcgggca cgtagatcgc ctgcacggag
     2641 gtgaccgaac ccttcttcgt cgacgtgatg cgctcctgca gctcgcccat ctccgttccg
     2701 agcgtcggct ggtagccgac cgccgacggc attcgcccga ggagcgccga gacctcggag
     2761 cccgcctggg tgaatcggaa gatgttgtcg atgaacagca ggacgtcctg cccttcctcg
     2821 tcccggaagt actccgcggc ggtgagcgcc gtcagcgcca cccggagccg cgagcccggc
     2881 ggctccgtca tctggccgaa gatgagggcc gtcttctcga ggacgcccga ctccttcatc
     2941 tcgtgataga ggtcgttgcc ctcgcgcgtg cgctccccca ccccggcgaa cacggagatg
     3001 cccccgtgct gcttggcgat gttgttgatc aactcctgga tcagcacggt cttgccgacc
     3061 ccggcgccgc cgaacagccc ggtcttgccg cccttcgtgt acggctcgag gaggtcgacg
     3121 accttgatgc cggtctcgaa catctcgacc ttcgtggact ggtcctcgaa cgcgggggcc
     3181 ggacggtgga tcgggtacgt cttcttcacc tggagcgcgg ggcccttgtc caccggctcg
     3241 cccacgatgt tgaggatgcg cccgagcgtc tccttgccga cggggaccga gatcggcgcg
     3301 ccggtgtcgg ccaccaccat gccgcgcgtg agcccgtcgg tggcggccat ggccaccgtg
     3361 cgcacctgac tctcgccgat gtgctgcgcg acctcgaggg tcagcttctg cgagtaggag
     3421 aagatgtcct tgttctccac gccctccacc acgagcgcgt tgtagatggc cggcaggcgc
     3481 ccgggctcga actccacatc gaccaccggc ccgatgacct ggacgatctt cccgttgctc
     3541 atgcttcggc tccttgcgtg agggcttcgg cgccgccgac gatgtcgagc agctccttgg
     3601 tgatcttctc ctgacgggcc ttgttgtact ggatggtcag gacgtcgatc atctccttgg
     3661 cgttcttggt cgccgcctcc atcgccgtca tgcgcgcgcc gtactcgccg gcgagcgact
     3721 ccatcagcgc gcggaacacc tgcatgcgca cgtgccgcgg cagcaggtcg ccgaggatgg
     3781 ccgcgggacc cggctcgaag atgtagtcca ccggcgtctc ggcgccgtcg ccacccgccg
     3841 cgtgcacgcg cgggatcggc aggagctgct ggcgcacggg ccgctgcacg gccaccgacc
     3901 ggaattcgtt gtagatcagg tggacctcgt ccacctcgcc gtccacgtac tgctgcatga
     3961 agtagtcggc cagctcctgg gcgtggctgt acgcgaggcg gtcccagaag ccgatcaggt
     4021 cgcgcttgat cgtccactgc cggcggcgat agaagtcgcg ggccttgcgc ccgacgacga
     4081 cgagggtcag gtcggtcggg ctcgtcgact cgcggaccgc ttccatcgcc ttgcggagga
     4141 tgttcgagtt gaaggcgccg gcgaggcccc ggtcggcggt gatgacgacg atctggcgcc
     4201 gcgggccctc gcgctgctcg agcagcgggt gcggcgcgcc ctcgctcccg gtaacgccga
     4261 ccaggttgcc cagcagctcg gccatcttcc cggcgtaggg gcgggcggcc aggatgcgct
     4321 cctgcgcccg ccggagcttc gcggcggcga cgagcttcat cgcgcgcgtg atcttctgcg
     4381 tggactggac ggaccggatc cgccgccgga tgtcgcgggg tgtcgccatc gcgcgctacg
     4441 ccgccttgac gctccgggcg gcgacgaact cggtcttggc ttcctgcacc gcggtcgtca
     4501 gctgctcgcg ggccgcgtcg ctgatctcct tcttgtcacg gatctcggcc aggacccggg
     4561 actgcctgcg ctcgagccag ccggcgaggt gctgttcgaa cgcccgcacc gcgtccaccg
     4621 gcaggtcgtc gagcaatccc tgcgtgcccg cgaagatgat cgccacctgc ttctcgaccg
     4681 ggagaggatc gtactggttc tgcttgagga cctccaccat gcgctggccg cgggcgagct
     4741 gcatctgcgt ggcgcggtcg aggtcggagc cgaactgggc gaaggcggcc agctcgcgga
     4801 actgggcgag gtccaggcgg agcttgccgg ccacctggcg catggccttg atctgcgccg
     4861 agccgccgac ccgcgagacg gagaggccga cgttcacggc cggacggatg ccgctgtaaa
     4921 agagatcggc ctcgaggtag atctggccgt cggtgatcga gatcacgttc gtcgggatgt
     4981 aggccgacac gtcaccgagc tgcgtctcga tgatgggcag cgccgtgagt gagccgccgc
     5041 caagctcgtc gttgagcttc gccgcgcgct cgagcagccg cgagtggagg tagaacacgt
     5101 ctccgggata cgcctcgcgc ccgggcggcc ggcgcagcag cagcgagagc tggcggtagg
     5161 ccgtggcgtg cttgctgagg tcgtcgtaga cgcacagggc gtgccgcttc gagtcgcgga
     5221 agtactcccc catcgtcacg cccgcgtagg gcgcgatgta ctgcagcggc gccggctccg
     5281 aggccgacgc gacgacgacg gtggtgtacg ccatggcgcc ggcgtcctcg aacaccttca
     5341 cgacctgcgc caccgtcgag cgcttctggc cgatggccac gtagaagcag aacacgtcct
     5401 ggcccttctg gttgatgatc gtgtccacgg cgatggcggt cttgcccgtc ccccggtcgc
     5461 cgatgatcag ctcgcgctgg ccgcg
//
LOCUS       DATIAZ010000355         9816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_769295, whole genome shotgun sequence.
ACCESSION   DATIAZ010000355 DATIAZ010000000
VERSION     DATIAZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769295"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..279
                     /locus_tag="VLF19_12085"
     CDS             121..279
                     /locus_tag="VLF19_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94038.1"
                     /translation="MARGALRYNHPVGRRPARHRHLSRPPFFERPGLLGHLVWGVPLA
                     GLTVLARS"
     gene            482..1972
                     /locus_tag="VLF19_12090"
     CDS             482..1972
                     /locus_tag="VLF19_12090"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSE94039.1"
                     /translation="MGFMDMRQWMGLLEKEGELRRITAEVDWDRELGAIARRVLEKKG
                     PALLFESIKGYRRGRCTKLLTGGLGDGRRLALALGFPKDAPNRELVQHVMRKNRETIA
                     PVVVRTGPVKEHIVRGDAVDQTEFPVPRWHYKEGGRYIHTFSAIVTRDPDTRVMNVGI
                     YRGMIGRKDTSPMLLIKGGQHWGQHFVKYAARGEPMPVACVIGWDPIMPFLAGSPVPA
                     GVCEWDVMGAYRGEPAELVRCETVDLEVPAAAEIVLEGFISDDPATYEIEGPFGEFTG
                     YVSDVPTPRPTMRVTCVTHRTDPVFRGCLEGTLPGSYSENSVMSSVQRAAIGWNILNG
                     AGIPGILDVWVPPITNGVNVHVQIRKHYQGQPKQIAAALWGNAAAQYRYKHVVVVEDD
                     IDPSSYEQVDWAFAHRVNAGEGGLVVFPGIFGSPIDPSTPAEDKDVHQLGTGLWNRVL
                     IDATRSWKHPRRPEWDDERFPPTVRPAPEDEARVRERWQELGLGDL"
     gene            2007..3122
                     /locus_tag="VLF19_12095"
     CDS             2007..3122
                     /locus_tag="VLF19_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSE94040.1"
                     /translation="MKITAVQAVPLAIPTRLTTPPSPWSAGTGKQLLVRVTTDDGLVG
                     WGECFAYGAPLAVANVVDDALAPLLLGKDPLQIEALTQTMHHALMIWGRRGLAMFAVS
                     GVELALWDLAGKARGVPVHALLGGVCRTELRAYTSLLRLTRAADVQRACAAATAQGFT
                     AIKLHQTDVESVAVARDVVGEDVTLMLDTNCPWSVEEAIRMGRRLEKYGLRWLEEPVW
                     PPEDYAGLARVRTAVAIPIATGENEATLHGIRHIVEAGAADIVQPSVTKVGGLGEMRK
                     IAALATAAGVTFQPHSFYFGPGLAATLHVIAATPGTPYVEFPVGALDASLTAEPILCR
                     GGVIPVPERPGLGADPDPNVLARYPYDRSSARPFYLI"
     gene            complement(3208..3579)
                     /locus_tag="VLF19_12100"
     CDS             complement(3208..3579)
                     /locus_tag="VLF19_12100"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HSE94041.1"
                     /translation="MWRTLAFYDIAAGLVGGLLAAVPGLIDYFTLVGRARTIGTYHMA
                     VNVATLALFAVSFVLRTHWGAPWVPAGSNLPQALTIIGAVLLGVGGWLGGQLVYVHGV
                     GVEAVDRAQAEAQEAKRRRAA"
     gene            complement(3504..3698)
                     /locus_tag="VLF19_12105"
     CDS             complement(3504..3698)
                     /locus_tag="VLF19_12105"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HSE94042.1"
                     /translation="MSTPASIGKHPIHPMQVVFPLGLLLAAVVFDVLTLLTGPTSGGR
                     SPSTTSPPASSGACLRRCLA"
     gene            complement(3751..4932)
                     /locus_tag="VLF19_12110"
     CDS             complement(3751..4932)
                     /locus_tag="VLF19_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSE94043.1"
                     /translation="MIQRARAVLGTACGAHFLHDGFSDIIYVLLPLWATEFGMSLTQV
                     GIIRTAYTGGMALFQIPAGLLAERWGERRLLAAGTAVTALGFIVASWAGGFAALLVVL
                     VLAGMGSGVQHPLSSSIISRAFEARGRRTALGTYNFSGDLGKVAASAGVGLAAALVGW
                     RAAGLAYGVLGLATALGLAVVLARLGAGEPAPAESGNGARASGWGIRDPRGFGALAAI
                     GMIDNATRTGFITFLPFALIAKGSSVAGIGAALALLFAGGAAGKFVCGLVAERVGVIR
                     TVILTETLTAVGILAVIASPLPVAWATLVPLGIALNGTSSVLYGTVADLVMPERRSRA
                     YGLYYTLSIGASSMAPSVYGLVGDAVGVAATLTIVASVVLAVIPLCLVLRPAVAAPVE
                     T"
     gene            5058..5681
                     /locus_tag="VLF19_12115"
     CDS             5058..5681
                     /locus_tag="VLF19_12115"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSE94044.1"
                     /translation="MTISITRVYTRTGDAGDTALVGGARVPKDSPRIEAYGTIDELNA
                     VVGLARTFNEERLSEGEHHRWLDDALKRIQNELFDLGSELATPPGAEYEGMFKVGEAQ
                     VRALEQLMDRCQKDLEPLRSFTLPGGGRVNAFLHQARTVCRRAERELLRLSRVEPIGE
                     WPLKYVNRLSDAFFVLSRWVARYAGEKEYLWERGLASHARPRARKRP"
     gene            5796..6272
                     /locus_tag="VLF19_12120"
     CDS             5796..6272
                     /locus_tag="VLF19_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSE94045.1"
                     /translation="MKARMSLTVNGETHEVLAAVHKTLLEVLREDLNLIGTKHGCELG
                     ECGTCTVLLDGEPALSCLLLPVEVEGRAITTVEGLAHGGRLHPLQQAFAELGAAQCGY
                     CTPGMLLTARALLDDTPTPTREQIKHALAGNLCRCTGYTKILDAVELAALRLGARR"
     gene            6269..8809
                     /locus_tag="VLF19_12125"
     CDS             6269..8809
                     /locus_tag="VLF19_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSE94046.1"
                     /translation="MSAEPRDSSAGCARASEGERPGQGPQPPSESKHGFTVVGRPLPK
                     IDAWAKVTGETRYADDLVLPRMAYAKLLRSAHPHAQIRRIDTTAARGVPGVYAVITGH
                     DLPRVKFGILPVSQDEEALCTEKVRMVGDAVAAVAAVDEETAERACRLVEVEYDRLPA
                     LMSIEDSLAHPEVRIHEYGDGPNVHKNVSLQFGDVEVAFARADLVREDVFFYEGNTHL
                     PMEQHAAVAQWGPDGKLTLWSSTQTPHYVHRLLARILDVPAAHIRVVAAPVGGGFGGK
                     LDPFAHEIAACKLSQLTGRPVKITLTREEVFYVHRGRHPVLMWLKTGFTKDGQITGSH
                     IKTWLDGGAYGSYGVASTFYTGVINPVTYKMPVYKFEGARVFTNKPPCGPKRGHGTPQ
                     PRFALEVQIDKAAEQLGLDPAEMRRRIVCEPFTKTANHLTVTTIGLGECIERVVEASG
                     WRDKWKGWSKSAGAGEAGARKTGIGIACSAYMTGAGTAIYWNSMPHSGVVLRADRSGG
                     VTILCGATDIGQGSDSILAYLPAEVLGIDPMDVHVHPADTSLTPVDLGSYSSRVTLMC
                     GMAAIQAARRLRAVMTEAVARKLQVDAGRLVFRDRKVGVPDDWDKAVPFPQAVELAEA
                     THGVLAFPGSYAPPKRAGKYKGGGVGPSPCYSYSACVVELTVDEETGEVELHDVWIGH
                     DVGRALNPLLVEGQVEGSVYMGIGEALMEGQVFRKGLHKQPSMLDYKSPTTLETPEIH
                     TILVETDDPEGPFGAKEAGQGPLLPVIPAIANAINDAVGVRVDELPIGPEQILRGLEL
                     KRQGKPGRVGPERIPLFKFKESLVVESAFGQPADAVAARPFADPEDVR"
     gene            9079..>9816
                     /locus_tag="VLF19_12130"
     CDS             9079..>9816
                     /locus_tag="VLF19_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSE94047.1"
                     /translation="MLRLPPFTYLAPKSVADAARLVAEHGREAMLVAGGTDLFPNMKR
                     GQFEPKVLVALSGIPELRGVRGDGRQGLSVGPTTTLTAVSRHPEVLRHYPGLATAAGL
                     VSSPQLRNVGTIGGNVCVDTRCNYYNQTYDWRKAIGFCMKKDGDICLVAPGSPRCWAV
                     SSSDTAPVLWSLGARVRLVGAAGERTIALRDLYRDDGIDHLAKRADEIVAEIVLPPAD
                     GWRSAYLKLRRRGSFDFPVLGVAVALRL"
BASE COUNT         1512 a         3422 c         3533 g         1349 t
ORIGIN      
        1 ccgtcaccga cgccgtcggc gcggcgtgct tcgccatcgt gctcgcttcg cagcgggccg
       61 gacgccgcga gcgcgtcgag acccttgaac cggccgccaa gtgacgcgcc ccatctcgcc
      121 gtggcacgcg gcgccctccg ctacaatcat ccggtggggc gacgacctgc tcggcatcgg
      181 cacctgtcca ggccgccgtt cttcgagcgg cccgggctgc tcgggcatct ggtgtggggc
      241 gttccgctcg ccgggctcac ggtgctggcg cggtcctgat cgacgtcgtc ggaaactgaa
      301 cgaggcgcgt cgcagctccg gaacctggcg gcgtgtcggg cgcgccacag catggctggg
      361 gcgtgccgag cacggggggc tcggggggca ttcggggcgc gggagtgtcg aggcgcgcca
      421 cagcgtggcg gggggcttga gggccatctc ggggccccga tgttggtagg agatccgaca
      481 catgggcttc atggacatgc ggcagtggat ggggcttctc gagaaggaag gcgagctccg
      541 gcggatcacc gccgaggtgg actgggaccg cgagctgggc gcgatcgccc gacgcgtgct
      601 ggagaagaag ggcccggcgc tgctcttcga gagcatcaag ggctacaggc gcggccggtg
      661 cacgaagttg ctgaccggcg ggctcggtga tggccggcgg ctggcgctgg ccctcggttt
      721 tcccaaggac gcgccaaacc gggagctggt ccagcacgtg atgcgcaaga accgggagac
      781 gatcgcgccg gtcgtcgtgc gtacgggccc cgtgaaggag cacatcgttc gcggcgacgc
      841 cgtcgaccag acggaatttc ccgtcccgag gtggcactac aaggaaggcg gacgctacat
      901 ccacaccttc tcggccatcg tcacgcgcga ccccgacacg cgcgtgatga acgtcggcat
      961 ctaccgcggc atgatcggcc gcaaggatac gagccccatg ctgctgatca agggcggcca
     1021 gcactggggc cagcacttcg tgaagtacgc ggcgcggggc gagccgatgc cggtcgcctg
     1081 cgtcatcggc tgggacccga tcatgccgtt cctggccggc tcgccggtgc ccgcgggcgt
     1141 gtgcgaatgg gacgtgatgg gcgcctatcg cggcgagccg gccgagctgg tccgctgcga
     1201 gaccgtcgat ctcgaggtgc cggcggcggc ggagatcgtg ctggagggct tcatcagcga
     1261 cgacccggcg acgtacgaga tcgaggggcc gttcggggag ttcacgggct acgtctccga
     1321 cgttcccacg ccgcggccga ccatgagggt gacctgcgtc acgcaccgca ccgaccccgt
     1381 cttccgcggc tgtctcgagg gcacgctgcc cggctcgtac agcgagaaca gcgtgatgtc
     1441 ctcggtgcag cgggcggcga tcggctggaa catcctgaac ggcgccggca tcccgggcat
     1501 tctcgacgtc tgggtgccgc cgatcaccaa cggtgtgaac gtccacgtgc agatccggaa
     1561 gcactaccag ggccagccca agcagatcgc cgccgccctt tggggtaacg cggccgcaca
     1621 gtaccgctac aaacacgtgg tcgtcgtcga ggacgacatc gacccgtcct cctacgagca
     1681 ggtggactgg gcgttcgctc accgcgtgaa cgccggcgag ggaggcctcg tcgtcttccc
     1741 cggcatcttc ggctcgccga tcgaccccag cacaccggcc gaggacaagg acgtccacca
     1801 gctcggcacc ggcctctgga atcgcgtgct catcgacgcc acgcgctcgt ggaagcaccc
     1861 gcggcgtccc gaatgggacg acgagcgctt cccgccgacg gtgcggccgg cgcccgaaga
     1921 cgaggcacgc gtgcgagagc gctggcagga gctggggttg ggcgacctgt gagcggctcg
     1981 ccgaggccct cggggatgac cgaaccatga agatcaccgc ggtgcaggcg gtgcctctcg
     2041 caatcccgac tcgcctgacg acgccgccct cgccgtggtc agcgggcacc ggcaagcagc
     2101 tgctcgtccg cgtgacgacc gacgacggtc tcgtcggctg gggcgagtgc ttcgcctacg
     2161 gtgcgcctct cgcggtggcc aacgtcgtcg acgacgcgct ggcgccgcta ctgctcggga
     2221 aggatccgct gcagatcgag gcgctcaccc agacgatgca ccacgcgctc atgatctggg
     2281 gccgccgcgg gctcgccatg ttcgcggtca gcggcgtcga gctggcgctc tgggacctgg
     2341 ccggcaaggc gcgtggcgtg cccgtccacg cgctgctcgg aggcgtgtgc cggaccgagc
     2401 tgcgagccta cacgagcctg ctccgcttga cccgggccgc cgacgtgcaa cgcgcgtgcg
     2461 cggcggcgac ggcgcagggc ttcaccgcca tcaagctgca ccagaccgac gtcgagtcgg
     2521 tcgccgtcgc ccgcgatgtc gtgggtgagg acgtcacgct gatgctcgac accaactgcc
     2581 catggtcggt cgaggaggcc atccggatgg ggcggcggct cgagaagtac gggttgcgct
     2641 ggctcgagga gccggtgtgg ccgccggagg actacgcggg cctggcgcgc gtgcgcacag
     2701 ccgtcgccat ccccatcgcc accggcgaga acgaggccac gctgcacggt atccgccaca
     2761 tcgtcgaggc gggagccgcc gacatcgtcc agccgagcgt gaccaaggtg ggcgggctcg
     2821 gcgagatgcg gaagatcgcc gcgctcgcga cggcggcagg ggtgacgttc cagccgcact
     2881 cgttctactt cggccccggg cttgcggcga ccctccacgt gatcgcggcc acgcccggga
     2941 ccccgtacgt ggagttcccg gtcggcgcgc tcgacgcgtc gctcaccgcg gagcccatcc
     3001 tgtgccgcgg cggcgtcatc cccgtgcccg agcgtccggg gctcggggca gatcccgatc
     3061 cgaatgttct cgctcgctat ccctacgacc gttcgtccgc caggcccttc tacctgatct
     3121 agaagaggga ggccccaaga aatggccccc ggaccatcgg gagtggcccg gcacgtcccg
     3181 gccaggccgt ggcgcgcgcc gccagtccta cgctgcgcgc cgccgcttcg cctcctgggc
     3241 ttcggcctgg gccctgtcga ccgcctcgac tccgacgcca tgaacgtaga cgagctggcc
     3301 tccgagccag ccgccgactc cgaggagcac ggcgccgatg atcgtgagcg cctgtggcag
     3361 gttcgagccg gccgggaccc acggggcgcc ccaatgggtg cggaggacga agctgacggc
     3421 gaagagcgcg agcgtcgcca cgttgacggc catgtgatac gtgccgatcg tccgggcgcg
     3481 tcccacgagc gtgaaatagt cgatcaggcc aggcaccgcc gcaagcaggc ccccgacgag
     3541 gccggcggcg atgtcgtaga aggcgagcgt ccgccagatg tcggccccgt gagcagcgtg
     3601 agcacgtcga acacgactgc cgccaggagc agcccgagcg ggaacaccac ctgcatcgga
     3661 tggatcggat gctttccgat cgacgcaggc gtactcatgc gcaccactgg ctgcatccgt
     3721 gatgccaccg agatctcggc tggcgccccg tcaggtttcc accggcgcgg ccaccgccgg
     3781 ccgtagcacg aggcagagcg gaatgaccgc aagcacgacg ctggcgacga tcgtcagcgt
     3841 cgccgccacg cccacggcat cgccgaccag cccgtagacg gacggcgcca tcgacgacgc
     3901 cccgatgctg agggtgtaat agagcccgta cgcgcgggac cgccgctcgg gcatgaccag
     3961 gtcggcgacg gtcccgtaga ggaccgacga ggtgccgttc agcgcgatcc cgagcggcac
     4021 cagcgtcgcc cacgcgaccg gcaggggcga ggcgatgacc gcgaggatcc cgacggccgt
     4081 cagcgtctcc gtcaggatga cggtgcgaat gacgcccacg cgctcggcga cgagaccgca
     4141 gacgaacttg cccgcggcgc cgccggcgaa gagcagcgcg agcgccgcgc cgatgccggc
     4201 gaccgacgag cccttggcga tgagcgcgaa gggcaggaag gtgatgaacc cggtgcgggt
     4261 ggcgttgtcg atcatgccga tcgccgccag cgcgccgaac ccgcgcgggt cgcggatgcc
     4321 ccaccccgag gcgcgcgcgc cgttgccgct ctcggcgggc gcgggctcac cggcgccgag
     4381 ccgcgcgagc acgaccgcca gcccgagcgc cgtcgccagc ccgagcacgc cgtaggccaa
     4441 cccggccgcg cgccagccca cgagcgcggc ggccagaccg acccccgcgg aggccgcgac
     4501 cttgccgagg tcgccggaga agttgtaggt gccgagcgca gtgcggcgtc cgcgcgcctc
     4561 gaaggcccgc gagatgatcg acgaggacag cgggtgctgc acgcccgagc ccatcccggc
     4621 cagcacgaga acgacgagca gcgcggcgaa gccgccggcc cagctggcca cgatgaagcc
     4681 gagcgcggtc accgcggtgc ccgcggcgag caaccgccgc tcgccccagc gctcggcgag
     4741 gaggccggcg gggatctgga acagggccat cccgcccgtg taggcggtcc ggatgatgcc
     4801 gacctgcgtg agcgacatgc cgaattcggt ggcccacagt ggcagcagca cgtagatgat
     4861 gtccgagaag ccgtcgtgga ggaagtgggc gccgcaggcg gtaccgagca cggcgcgggc
     4921 gcgctggatc acgccgcgat catctcgcga cccctcgcgg tgttcaagtg ccgagcgcgc
     4981 ggggctcgcg tgggcccggc ggcgttccat gcggtcctcg gcggtcgagt agaataccgt
     5041 ccaccacgga ggccgccatg accatcagca tcacccgggt ctacacgcgc accggcgacg
     5101 cgggcgacac ggcgctcgtg ggcggcgccc gcgtgcccaa ggactcgccg cgcatcgagg
     5161 cctacgggac catcgacgag ctcaatgccg tcgtcggcct ggcccgcacg ttcaacgagg
     5221 agcgtctgag cgagggcgag catcaccgct ggctcgacga cgcgctcaag cggatccaga
     5281 acgagctgtt cgacctcggc agcgagctgg cgacgccacc gggcgccgag tacgagggca
     5341 tgttcaaggt gggcgaggcc caggtgcggg cgctcgagca gctcatggac cgctgtcaga
     5401 aggacctcga gccgctccgc tcgttcacgc tgccgggtgg cgggcgcgtc aacgcgttcc
     5461 tgcaccaggc gcggacggtg tgccgccggg ccgagcgcga gctgctgcgc ctgtcccggg
     5521 tggagccgat cggcgagtgg ccgctgaagt acgtcaaccg gctgagcgac gctttcttcg
     5581 tgctctcccg ctgggtcgcc cggtacgccg gcgagaagga atatctctgg gaacgggggc
     5641 tcgcgagcca cgcgcgcccg cgtgcccgca agcgcccctg aagaaattcg cccggcgcgc
     5701 gcccgggtcg agacccgccg ccccggcttg acggggccgt cccgattcag tatttttgaa
     5761 ccacccttaa tctacaatga actgggagtg gccgcatgaa ggcgcggatg agtctcaccg
     5821 tcaacggtga gactcacgag gtcctggccg ccgtccacaa gacgctgctg gaggttctgc
     5881 gcgaggacct gaatctcatc gggaccaagc acggctgcga gctgggcgag tgtggcacgt
     5941 gcaccgtgct cctcgacggc gagccggcgc tgtcgtgcct gctgctgccg gtcgaagtcg
     6001 agggacgcgc gatcaccacg gtggaaggcc tcgcgcacgg gggccggctc catccgctgc
     6061 agcaggcgtt cgccgagctg ggcgccgccc agtgcgggta ctgcacgcct ggcatgctcc
     6121 tgaccgccag ggcgctcctg gacgacaccc ccacgccgac ccgcgagcag atcaagcacg
     6181 cgctcgcagg caacctctgc cgctgcaccg gctacacgaa gatcctcgac gccgtcgagc
     6241 tggcggccct gcggctggga gctcgtcgat gagtgctgag ccgagggatt cgagcgcggg
     6301 gtgtgcccgc gcctccgagg gggagaggcc cggacagggg ccgcagcccc cctccgagag
     6361 caagcacggg ttcaccgtcg tcggccggcc gctgcccaag atcgacgcct gggcgaaggt
     6421 caccggcgag acgcgctacg ccgacgatct cgtgctgccg cgcatggcct acgccaagct
     6481 cctgcgctcg gcccacccgc atgcccagat ccggagaatc gacacgacgg ccgcgcgcgg
     6541 cgtgcccggg gtgtacgccg tcatcacggg ccacgacctg ccccgggtga agttcggcat
     6601 cctgcccgtc tcgcaggacg aggaggcgct gtgcaccgag aaggtgcgaa tggtcggcga
     6661 cgccgtcgct gccgtcgccg ccgtcgacga ggagacggcc gagcgcgcct gccgcctcgt
     6721 cgaggtcgag tacgaccggc tgccggcgct catgtccatc gaggactcgc tcgcccatcc
     6781 cgaggtccgc atccacgagt acggcgacgg ccccaacgtc cacaagaacg tctcgctgca
     6841 gttcggcgac gtggaggtgg ctttcgcccg ggccgacctc gtgcgcgagg acgtcttctt
     6901 ctacgagggc aacacgcacc tgccgatgga gcagcacgcg gcggtggccc agtggggtcc
     6961 ggacggcaag ctgacgctct ggtcctcgac ccaaacgccg cactacgtgc accggctgct
     7021 cgccaggatc ctcgacgtgc cggccgccca catccgcgtc gtcgccgcgc cggtcggtgg
     7081 cgggttcggc ggcaagctcg atcccttcgc ccacgagatc gccgcgtgca agctctcgca
     7141 gctcacgggc cggcccgtga agatcaccct cacgcgggag gaggtcttct acgtccatcg
     7201 cggccggcac cccgtgctga tgtggctcaa gacggggttc acgaaggacg ggcagatcac
     7261 gggttcgcac atcaagacgt ggctcgacgg cggcgcctat ggctcgtacg gcgtggcctc
     7321 gaccttctac accggcgtca tcaacccggt gacctacaag atgccggtct acaagttcga
     7381 gggcgcgcgc gtgttcacca acaagccgcc ctgcggtccc aagcgcggcc acggcacccc
     7441 gcagccgcgc ttcgcgctcg aggtgcagat cgacaaggcc gccgagcagc tcgggctgga
     7501 cccggccgag atgcggcggc ggatcgtctg cgagccgttc acgaagacgg ccaaccacct
     7561 cacggtgacg acgatcgggc tcggcgagtg catcgagagg gtcgtcgagg cctccggctg
     7621 gcgcgacaag tggaaaggct ggagcaagtc ggcgggggcc ggcgaagccg gcgctcgaaa
     7681 gacggggatc gggatcgcgt gctcggcgta catgacgggc gcggggacgg cgatctactg
     7741 gaacagcatg ccgcactccg gcgtggtgct gcgtgccgac cggagcggcg gcgtgacgat
     7801 cctgtgcggc gcgacggaca tcggccaggg ctccgactcg atcctcgctt acttgccggc
     7861 cgaggtgctg ggcatcgacc ccatggacgt gcacgtgcat cccgccgaca cgagcctgac
     7921 accggtggac ctcgggtcct actcgtcgcg cgtgacactc atgtgcggca tggccgccat
     7981 ccaggcggca cgtcggctcc gcgccgtcat gacggaggcc gtggcgcgga agctccaggt
     8041 cgacgccgga cggctggtgt tccgggaccg caaggtcggc gtgccggacg actgggacaa
     8101 ggccgtgccg tttccgcagg ccgtcgagct ggcggaggcg acgcacggcg tgctcgcgtt
     8161 ccccgggtcg tacgcgccgc ccaagcgcgc gggcaagtac aagggtggcg gcgtcggacc
     8221 gtcgccctgt tactcgtact ccgcgtgcgt ggtcgagctg acggtcgacg aggagaccgg
     8281 agaggtcgag ctgcacgacg tgtggatcgg acacgacgtc ggccgcgccc tgaacccgct
     8341 gctggtcgag ggtcaggtcg agggctccgt gtacatgggc atcggcgagg cactcatgga
     8401 gggccaggtc ttccgcaagg gcctgcacaa gcagccgtca atgctcgact acaagagccc
     8461 gacgacgctc gagaccccgg agatccacac gatcctcgtg gagaccgacg atcccgaggg
     8521 accgtttggc gccaaggaag cggggcaggg cccgctgctg cctgtcattc ccgccatcgc
     8581 caacgccatc aacgacgccg tcggtgtgcg cgtggacgag ctgccgatcg ggcccgagca
     8641 gatcctcagg ggcctcgagc tgaagcgcca gggcaagccg gggcgtgtcg ggccggagcg
     8701 catcccgctg ttcaagttca aggaatcgct ggtcgtcgag tccgcgttcg gccagcctgc
     8761 cgacgccgtt gcggcgcgcc cgttcgccga tccagaggac gtgcgctagt gtccgcgatc
     8821 ggagctcgcg ggtgaaggcg ccggccgggg tggggccggg gcggggggcg cgggtccggc
     8881 gacccctgac gcgttgcgcg tggtcctgcg agtgttattt ccatcgtcgg gtgtggctgc
     8941 ggttccgtgg gcggggaaca ggtcggcgga cccgcgcccc ccgccccggc cccaccccgg
     9001 ccggcggtcg gcgagtcttg gaatcggcac acttggtgat cgtgattcga actgtgggcg
     9061 cgggctcggg acactagcat gctgcggctg ccgccgttca cgtatcttgc gccgaagagc
     9121 gtcgcagatg ccgcacgact cgtcgccgag cacgggcgcg aggcgatgct cgtcgccggc
     9181 ggcaccgatc tctttcccaa catgaagcgc ggccagttcg agccgaaagt gctcgtggcg
     9241 ctgagcggga tccccgagct gcgcggcgtg cggggagacg gtcgccaggg gctgtccgtg
     9301 gggccgacga cgacgctgac agccgtgtct cgccaccccg aggtcctgcg ccactacccc
     9361 gggctggcga cggcggcagg gctcgtgtcc tcgccgcagc tccgcaacgt gggcaccatc
     9421 ggcggcaacg tgtgcgtgga cacccgctgc aactactaca accagacgta cgactggcgg
     9481 aaggcgatcg gcttctgcat gaagaaggac ggcgacatct gtctcgtcgc ccccggcagc
     9541 ccgcgctgct gggcggtgtc gtcgtccgac accgcgccgg tgctgtggag cctcggagcg
     9601 cgcgtccgcc tggtcggcgc cgcgggcgag cgtacgatcg ccctccgcga cctctatcgc
     9661 gacgacggca tcgatcacct ggccaagcgg gccgacgaga tcgtcgccga gatcgtgctg
     9721 ccgccggccg acggctggcg ctcggcctac ctcaagctgc gacggcgggg ctcgttcgac
     9781 tttcccgtcc tcggcgtggc cgtcgcgctg cgcctc
//
LOCUS       DATIAZ010000356         6736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_903344, whole genome shotgun sequence.
ACCESSION   DATIAZ010000356 DATIAZ010000000
VERSION     DATIAZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903344"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..582
                     /locus_tag="VLF19_12135"
     CDS             136..582
                     /locus_tag="VLF19_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSE94048.1"
                     /translation="MSRTDVTIRPAESTDLDDIARIDETLAGRTRKDYWQTRLDIAAL
                     RPPWMSLVAETDGRVVGFLFGWVGESEFGIAGGTGWIDLIGLDPRYRGLGIGRALVER
                     FVASGRELRAIQKVATLVDLAQTDVRDFFGRLGLRHGPMVQLEKPA"
     gene            647..1798
                     /locus_tag="VLF19_12140"
     CDS             647..1798
                     /locus_tag="VLF19_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSE94049.1"
                     /translation="MRLITALLTAALTATAAPALGEEAYRIGAIFSITGPGSSLGIPE
                     RDTALMLEADINGRGGVKGPDGKARPLKIVVYDDGSDETKAVLAAKKLIDEDKVVAIV
                     GTTLSGTSLAILDTVQKAEVPMISCAAAAKIVEPAAERKWVFKTPQSDQLIVGVLADD
                     LKARGLTKVGWLNVDYAFGQLGWIEFEKAAQKLGLAVVANEKFGQKDVDVTAQLVRVK
                     AAGPQAVVIWSIPPSASIATKNYGDLGLTAPLFQSHGVGNRKYIELAGAAANGVRFPA
                     GKLLVAEQLPDGDPQKGVLLAYARDFEAKYGPRNTFGGHAWDAVQLAVKAIAQAGADR
                     ARVRAAIESTRNFVGITGVFDFSPTDHNGLDRRAVTMIQIVDGQWKPAR"
     gene            1802..2683
                     /locus_tag="VLF19_12145"
     CDS             1802..2683
                     /locus_tag="VLF19_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE94050.1"
                     /translation="MPSAEQLAQYLASGLVVGGVYALIGLGFVIVYSVTRIVNFAQGE
                     MVMLGALGMVTLVDRGLGIPVAFALTVGGVAALGAVLERVAVHPLRAAPALTVLIVTI
                     GASIALRGLALVAWGTDPFALRPFSAGPPVHVLGAVIVRQGLWVLALAALVFAGLWLF
                     FTWTYAGIAVRACAVSPRAARLMGIRVDRVFLLAFALAAALGATAGAVVAPITYATYD
                     MGLMLGLKGFVAAVLGGLVSPPGAIAGGFLLGILESFGAGLVSSGYKDAVAFVILIAL
                     CLVQVAGWLPWRVAEEG"
     gene            2690..3646
                     /locus_tag="VLF19_12150"
     CDS             2690..3646
                     /locus_tag="VLF19_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSE94051.1"
                     /translation="MRAPARWSVLAGAGLAVALLPSIVSSRYLLGTLVFIGLNALAAL
                     GLSLVMGFAGQVSLGQAAFFAVGAYTSGVLTASHGWNGWAALALAAGAGALTAFLVGL
                     PIFRLSGLLLAMASLGFGIIVYYVLVNWVPVTGGPSGLTGIPPLALGSFAFSTDRRML
                     WLVWAAVLLALGLCGNLVDSRVGRALRAIHGSEAAAQAAGIDVTRLKLGVFALAGGLT
                     ALAGGLYAHYLAFINPSPFGFAYSVELVVMVVLGGVASLWGAVLGAAVVVVLVEALRA
                     LLPLLTASHGAAEYEIVVFGLILMAFMVFLPGGLTRFRETRA"
     gene            3643..4413
                     /locus_tag="VLF19_12155"
     CDS             3643..4413
                     /locus_tag="VLF19_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE94052.1"
                     /translation="MSGRPLLEVQGLARHFGGVAAVRGVDFTVATGQIKALIGPNGAG
                     KTTIFNIIAGVLPPTRGRVLFDGVPLPTCRPHAVAARGIARTFQLVRLFPQMTVLEHA
                     LVGCHRHGRAGWGACALRTRAMRDEERRLRERAREVLARVGLEARADVAAGALTYGEQ
                     RLVELARALAMAPRLLLLDEPGAGLDGQEQARLGALIRDVRRGGTTVLLVDHHMDLVM
                     DLSDEVLVLSYGEKLAEGRPDEVRAQPAVVAAYLGEEA"
     gene            4413..5144
                     /locus_tag="VLF19_12160"
     CDS             4413..5144
                     /locus_tag="VLF19_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE94053.1"
                     /translation="MLAVRDVDAGYGHVQALRRVSLRVAPGELVTIVGPNGAGKTTLL
                     RAITGLVPVRAGTIAFEGTPIGGARTEDIVRLGVALVPEGRDLFGPLSVRDNLALGAY
                     ARSRGARRAGLAADLERVFALFPALRARLGQAAATLSGGEQQMVAIGRALMARPRLLL
                     LDEPSVGLAPLVIREIFRALAILKAEGLTILLVEQNARAAFRIADRGYVLRPGGVMAE
                     VGANGHGADPARAYGFAASTREAPS"
     gene            5141..6451
                     /locus_tag="VLF19_12165"
     CDS             5141..6451
                     /locus_tag="VLF19_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HSE94054.1"
                     /translation="MTEALPLEHLDREALNALQLQRLRRVVAWVSDRVPLYRERLRAA
                     GVAADDVRSLADLPQLPCTAKADFRDTYPYGMLAVPLESVVRIHASSGTTGKPTVVAY
                     TRRDLDTWTAVMARTLAMGGVGAADVVHNAYGYGLFTGGLGFHYGAEALGAAVIPISG
                     GFTERQLTALTDLGSTVLCSTPSYALHLAEALEDTGVAAKDLRLRVGFFGAEPWTEGM
                     REALESRLDLMALNIYGLSEVMGPGVSVECPARRGMHVAEDHFLPEVVDPVTLEPLPP
                     GTPGELLLTTLTKEALPVLRYRTRDLTAIEPDPCPCGRTLVRIRRITGRTDDMLIIRG
                     VNVYPSQIEHVLTQVPQVDPQYLLVVRREGALDTLEVRVEVRPEVAGAGDGALRDAAR
                     HVRRRVHEVIGLTVDVTALPPRTLERAPGKARRVQDLRTQGGAA"
     gene            6448..6609
                     /locus_tag="VLF19_12170"
     CDS             6448..6609
                     /locus_tag="VLF19_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94055.1"
                     /translation="MSRSRVWGGNGLPCNGSLDQLDARGLRPGARQKPVRDDDCALFS
                     LELPAGGVR"
BASE COUNT          811 a         2488 c         2435 g         1002 t
ORIGIN      
        1 agcgcttccg caagcgggcc gccgcggccg ccgcctgagc ccgcggacgg atcgtggagt
       61 cgaggggcgc cacggcgccc ggagcaccga aggcccgggg gagcccggag cctccatgga
      121 cagggaggtc cgacgatgag ccgcaccgac gtgacgatcc ggccggccga gtcgaccgat
      181 ctcgacgaca tcgcgcgcat cgacgagacg ctcgccggcc ggacccgcaa ggactactgg
      241 cagacccggc tggacatcgc cgcgctccgc ccgccgtgga tgtcgctcgt ggcggagacc
      301 gacggccgtg tcgtcggctt cctgttcggc tgggtcggcg agtccgagtt cggcatcgcc
      361 ggcggcaccg gctggatcga cctcatcggc ctcgacccgc gctatcgcgg tctcgggatc
      421 gggcgcgcgc tcgtggagcg cttcgtggcc agtggccgcg agctacgcgc catccagaag
      481 gttgccacgc tcgtggacct cgcgcagacc gacgtccgtg acttcttcgg ccgcctcggg
      541 ctgcgccacg ggccgatggt gcagctggag aaaccggcgt gaggcccgac cccgatcgcc
      601 acgacacccg gcgcgacctc gcccccagga tcgagaggag gatcccatgc gcctgatcac
      661 cgccctgctc accgccgctc tgaccgcgac cgccgcgccc gccctgggcg aggaggcgta
      721 ccggatcggc gcgatcttct ccatcacggg ccccggctcc tcgctcggca tccccgagcg
      781 cgacaccgcg ctcatgctcg aggccgacat caacgggcgg ggcggggtca agggccccga
      841 cggcaaggcc cgtccattga agatcgtcgt gtacgacgac ggcagcgacg agacgaaggc
      901 cgtgctggct gccaagaagc tcatcgacga ggacaaggtc gtggccatcg tcggcaccac
      961 gctctccggc acctcgctcg ccatcctgga caccgtgcag aaggccgagg tgcccatgat
     1021 ctcctgcgcg gcggccgcca agatcgtgga gcccgcggcg gagcggaagt gggtcttcaa
     1081 gacgccgcag agcgaccagc tgatcgtcgg cgtcctcgcc gacgacctca aggctcgggg
     1141 cctcacgaag gtcggctggc tcaacgtcga ctacgcgttc gggcagctcg gctggatcga
     1201 gttcgagaag gccgcgcaga agctcggcct ggccgtcgtc gccaacgaga agttcggcca
     1261 gaaggacgtg gacgtgacgg cgcagctcgt gcgggtgaag gcggccggtc cccaggccgt
     1321 cgtgatctgg tcgatcccgc cgtcggcctc catcgcgacg aagaactacg gggacctcgg
     1381 gctcacggcg ccgctgttcc agagtcacgg cgtcggcaac cggaagtaca tcgagctggc
     1441 cggcgccgcc gccaacggcg tacgcttccc cgccggcaag ctgctcgtcg ccgagcagct
     1501 gccggacggc gacccgcaga agggcgtgct gctcgcctac gcccgggact tcgaggcgaa
     1561 gtacggtccg cgcaacacct tcggcggcca cgcctgggac gccgtgcagc tcgccgtcaa
     1621 ggccatcgcg caggccggcg ccgaccgcgc ccgggtgcga gcggcgatcg agtccacgcg
     1681 caacttcgtc ggcatcaccg gcgtcttcga cttctcgccg acggaccaca acggtctcga
     1741 ccgacgcgcc gtgacgatga tccagatcgt cgacggccag tggaagccgg cgaggtaggc
     1801 gatgccctcc gccgagcagc tcgcccagta cctggcctcc ggtctggtgg tgggcggcgt
     1861 ctacgcgctg atcggcctcg gcttcgtgat cgtctacagc gtgacgcgca tcgtcaactt
     1921 cgcccagggc gagatggtga tgctgggcgc gctcgggatg gtgaccctgg tcgatcgcgg
     1981 gctcgggatc cccgtcgctt tcgcgctcac ggtcggcggg gtcgccgccc tgggcgcggt
     2041 gctggagcgg gtggccgtgc acccgctccg cgcagctccg gcgctcaccg tgctgatcgt
     2101 gaccatcggc gcctcgatcg cgctgcgcgg gctcgccctg gtggcctggg gcaccgaccc
     2161 cttcgcgctg cggccgttct ccgcggggcc acccgtccac gtgctcggcg cggtgatcgt
     2221 ccgccagggg ctgtgggtgc tcgcgctcgc cgcgctcgtc ttcgccggcc tctggctctt
     2281 cttcacgtgg acctacgcgg gcatcgcggt gcgcgcttgc gcggtgagcc cgcgcgcggc
     2341 gcggctgatg ggcatccgcg tggaccgcgt gttcctgctg gcattcgcgc tggccgccgc
     2401 gctcggcgcc accgcgggcg ccgtcgtggc ccccatcacc tacgccacct acgacatggg
     2461 gctgatgctg gggctcaagg gcttcgtcgc cgcggtgctc ggcggcctcg tgagcccgcc
     2521 cggcgccatc gcgggcggct tcctgctcgg catcctcgag tcgttcggcg ccggcctcgt
     2581 ctcctccggc tacaaggatg ccgttgcgtt cgtgatcctc atcgcgctct gcctcgtcca
     2641 ggtcgccggc tggctgccct ggcgcgtggc cgaggagggc tgaggcgcca tgcgcgcgcc
     2701 cgcgcgctgg agcgtcctgg ccggcgctgg cctcgcagtg gcgctgctgc cgtcgatcgt
     2761 gagctcccgc tacctgctgg ggacgctggt cttcatcggg ctcaacgccc tggccgccct
     2821 cggcctctcg ctggtgatgg gcttcgccgg ccaggtgtcg ctgggccagg cggcgttctt
     2881 cgcggtcggc gcctacacga gcggggtgct gacggcctcc cacggctgga acggctgggc
     2941 ggccctcgcg ctggccgcgg gcgcgggcgc gctgacggcc ttccttgtgg gcctgcccat
     3001 cttccggctc tccggcctgc tgctggccat ggcgtcgctc gggttcggca tcatcgtcta
     3061 ctacgtgctc gtcaactggg tgcccgtgac cggcgggccg tcgggcctca cgggcatccc
     3121 gccgctggcg ctcggcagct tcgccttctc caccgaccgg cggatgctgt ggctcgtgtg
     3181 ggcggccgtg ctgctggcgc tcgggctgtg cggcaacctc gtggactctc gcgtgggtcg
     3241 cgcgctgcgc gccatccacg gcagcgaggc cgccgcgcag gcggcgggga tcgacgtcac
     3301 ccgcctcaag ctgggagtct tcgcgctcgc cggcggcctc accgcgctgg ccgggggcct
     3361 gtacgcgcac tacctggcct tcatcaatcc ctcgccgttc ggcttcgcat actcggtgga
     3421 gctggtggtc atggtggtcc tcggcggcgt cgccagtctc tggggggcgg tgctcggcgc
     3481 cgcggtggtg gtcgtgctcg tggaagcgct acgcgcgctg ctgccgcttc tcaccgcctc
     3541 gcacggcgcc gccgagtacg agatcgtcgt cttcggcctg atcctcatgg ccttcatggt
     3601 cttcctgccc ggcggactga cgcgattccg ggagacccgc gcgtgagtgg ccgaccgctg
     3661 ctcgaggtcc agggcctcgc ccggcacttc ggcggcgtgg cggcggtgcg cggcgtcgac
     3721 ttcaccgtcg cgaccggcca gatcaaggca ctcatcggcc cgaacggcgc cggcaagacg
     3781 acgatcttca acatcatcgc cggcgtgctg ccacccacgc gcggccgcgt gctcttcgac
     3841 ggcgtgccgc tcccgacctg ccggccccac gccgtcgccg cgcggggcat cgcgcgcacg
     3901 ttccagctcg tgcgcctctt cccccagatg accgtgctcg agcacgcgct ggtcggttgc
     3961 caccgccacg ggcgggccgg ctggggagcc tgcgccctgc ggacgcgggc gatgcgcgac
     4021 gaggagcggc ggctgcgcga gcgggcgcgc gaggtgctgg ctcgcgtcgg cctcgaggcg
     4081 cgcgccgacg tcgccgccgg cgcgctgacc tacggcgagc agcggctcgt cgagctggcg
     4141 cgcgcgctgg ccatggcgcc gcggctgctc ctgctcgacg agcccggggc cgggctggac
     4201 ggccaggagc aggcgcggct gggggcgctg atccgtgacg tgcgccgcgg ggggacgacc
     4261 gtgctcctcg tcgatcacca catggatctc gtcatggacc tctccgacga ggtcctcgta
     4321 ctatcgtatg gcgagaagct cgccgagggc cggccggacg aggtgcgggc gcagccggcc
     4381 gtggtcgccg cgtacctggg cgaggaggcg tgatgctcgc cgtccgggac gtcgacgccg
     4441 gctacggcca cgtgcaggcg ctgcgccgcg tgagcctccg cgtcgcgccc ggcgagctgg
     4501 tgacgatcgt gggccccaac ggcgccggca agaccaccct gctgcgagcg atcacggggc
     4561 tggttcccgt ccgcgccggg accatcgcct tcgagggcac gccgatcggc ggggcgcgca
     4621 ccgaggacat cgtgcggctc ggcgtggcgc tggtacccga ggggcgcgac ctcttcgggc
     4681 cgctcagcgt gcgcgacaac ctcgccctcg gcgcgtacgc gcgttcccgc ggggcgcgcc
     4741 gcgccggtct ggccgccgac ctcgagcgcg tgttcgcgct cttccccgcc ctgcgcgcgc
     4801 ggctcggcca ggcggctgcg acgctgtcgg gcggcgagca gcagatggtg gcgatcgggc
     4861 gcgcgctcat ggcacgaccg cgcctgctgc tgctcgacga gccctcggtg ggcctggcgc
     4921 cgctcgtcat ccgcgagatc ttccgcgccc tcgcaatctt gaaggccgag ggcctcacca
     4981 tccttctcgt cgagcagaac gctcgcgcgg ccttccgcat cgccgaccgg ggctacgtgc
     5041 tccgccccgg cggcgtgatg gccgaggtcg gcgccaacgg ccacggcgcc gacccggcgc
     5101 gcgcgtacgg gttcgccgcg agcacacggg aggcgccgtc atgacggaag ccctgccgct
     5161 ggagcatctg gaccgcgaag ccctgaacgc cctgcagctg cagcgcctgc gacgagtggt
     5221 ggcgtgggtg tcggaccgcg tgccgctcta ccgcgagcgg ctgcgcgcgg ccggggtcgc
     5281 cgccgacgac gtccgctcgc tcgccgacct gccgcagctg ccctgcaccg ccaaggccga
     5341 cttccgcgac acctacccgt acggcatgct ggcggtgccg ctcgagtccg tcgtgcggat
     5401 ccacgcctcg tccggcacca cgggcaagcc cacggtcgtg gcctacaccc ggcgcgacct
     5461 cgacacgtgg accgccgtca tggcgcgcac cctcgcgatg ggcggcgtgg gggccgccga
     5521 cgtcgtgcac aacgcgtacg gctatggcct cttcacgggc gggctcggct tccactacgg
     5581 cgccgaggcg ctcggcgccg cggtcatccc gatctcgggc gggttcaccg agcgtcagct
     5641 gacggcgctc accgatctcg gctcgacggt cctctgctcc acgccgtcct acgcgctgca
     5701 cctcgcggag gcgctggaag acaccggcgt cgccgcgaag gacctccggc tgcgggtcgg
     5761 cttcttcggc gccgagccat ggaccgaggg catgcgcgag gcgctcgaga gccgactcga
     5821 cctcatggcg ctgaacatct acggcctcag cgaggtcatg ggccccggcg tcagcgtcga
     5881 gtgtccggcg cggcggggaa tgcacgtcgc cgaggaccac ttcctgccgg aggtcgtgga
     5941 ccccgtgacc ctcgagccgc tgccgcccgg gacgccgggc gaactgctgc tgacgacgct
     6001 caccaaggaa gccctgccgg tcctgcgcta tcgcacgcgc gatctcaccg cgatcgagcc
     6061 cgatccgtgc ccctgcgggc ggaccctcgt gcgcatccgc cgcatcaccg ggcgcacgga
     6121 cgacatgctc atcatccgcg gcgtcaacgt ctacccctcg cagatcgagc acgtcctgac
     6181 ccaggtgccc caggtggatc cgcaatacct gctcgtcgtg cgccgcgagg gtgcgctcga
     6241 caccctggag gtgcgggtcg aggtgaggcc agaggtggcc ggcgcgggcg acggcgcgct
     6301 gcgcgacgcc gcgcgccacg tccggcggcg cgtccacgag gtgatcggcc tcacggtgga
     6361 cgtcaccgcg ctgccgccgc ggacgctcga gcgcgccccg ggcaaggcgc gccgggtcca
     6421 ggacctccgg acccagggag gtgcagcatg agcaggtcgc gcgtatgggg cggcaacggc
     6481 ctgccgtgca atgggagcct ggaccagctc gacgcacggg gcctgcggcc cggagcccgg
     6541 cagaagccgg tccgcgacga cgactgcgcg ctgttcagtc tcgagctgcc ggcaggaggt
     6601 gtgcgatgaa tccgtggctg gtgctcggag cgatcgtcct cattgcggtg ctcttcgtgc
     6661 tgctccccgt cggcgcagcg gggtactctc ggttccggcg tcgctggcgt ctcccgtgcc
     6721 ccgcggaggg catcga
//
LOCUS       DATIAZ010000357         5717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_244115, whole genome shotgun sequence.
ACCESSION   DATIAZ010000357 DATIAZ010000000
VERSION     DATIAZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244115"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VLF19_12175"
     CDS             <1..216
                     /locus_tag="VLF19_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF993 family protein"
                     /protein_id="HSE94056.1"
                     /translation="APTRFYKTGVVFAAYLNGHQRHFRMVGGLESARSVVHLARQFVL
                     MDQAGLLRDPEAAAERMRRTLAVAGVE"
     gene            complement(275..418)
                     /locus_tag="VLF19_12180"
     CDS             complement(275..418)
                     /locus_tag="VLF19_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94057.1"
                     /translation="MTDTTPSGARPRFGVGSYISRCYGSLTTFNVLFGAAEGQLEGQG
                     DRE"
     gene            complement(485..1021)
                     /locus_tag="VLF19_12185"
     CDS             complement(485..1021)
                     /locus_tag="VLF19_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94058.1"
                     /translation="MIVVHVAFTCAEDYVARREPHRHAHIERLAGLRSIGAVIGGGPA
                     PDGRGADVVYRLQQPAQLEPAIEEDPYWTAGAWTGYTPRSFSQFVEPWQAAEIVLDGS
                     RRATVVEGRVADPDMAEFALIELRGAGRLAFGGFFEDGETFALCRTAEADEALGWFRD
                     TAFWAADTLRARPLLWVL"
     gene            1338..3941
                     /locus_tag="VLF19_12190"
     CDS             1338..3941
                     /locus_tag="VLF19_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSE94059.1"
                     /translation="MALLERFRARYPFPLDDFQVEACRVIESGGSVIVSAPTGAGKTL
                     VAEFAIQMALETGRRLAYTTPLKALSNQKFADFTRALGEDTVGILTGDVKVNPQGRVL
                     VMTTEILRNMLYGGGLADLGWIVLDECHYMGDEGRGTVWEEIIVNAPADVPLVALSAT
                     VANVREIADWISLVHRPIAPIYHPHRPVPLSYGLADLTGEIHEFDKVRTGRARVIGDE
                     RPGADERGRWYSRRVVDPTVLIEALETRGWLPAIYFIFSRAGCERAMDDVLVDGRALL
                     SREQQGEVDRTIGEALRESPTMGESEINQTIFRALGLGVAVHHAGILPSVKRLIERLF
                     ERGLCKVVFATETMSLGIHMPARAVVLQGLTKRTERGFRSLTHNELTQMAGRAGRRGI
                     DAEGQCVIALDARDGVEDVVRVVDSAPEPIASQFKLGYGSVALLLATGAEPEVLRRRI
                     EASFGQYQNVKRIRELEDGLARLEAALAEARAFAAPCGDFERIGRYRRLRQEVDARRR
                     AMGRGARRGERGVIEAEPGRLVLLRRKGGPSLAMVLAVHGIRGHRALLEALLPHGAVV
                     RVKAGVIKQVFWSTPTLAVPRDAHAGARGREGRGLRHLADQLARTDVAALIERERAHR
                     PEASLGSVECHRCPWGSTPHCDAAWREVERLSERVAQRRGALDAVRGAYWQEFLRVVE
                     VLEQFGAVRGGWLEAKGRLVAGLRHDNELLVAEVVTRGILTDLTVAEAAAVCSALGEE
                     SRSGEPMIARAFLRSRPKLRRKLEHLGAAAEAVAQAQRQRHLGMPVAVHPGFMPAVFR
                     WVSGDDDWSGIVQQSFGGHEGDLIRAMRRLIDLLRQLGESAEVPATTGRLLLQAARLV
                     DRGIVLESALI"
     gene            3954..4451
                     /locus_tag="VLF19_12195"
     CDS             3954..4451
                     /locus_tag="VLF19_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94060.1"
                     /translation="MLMTVTPRVLGLVCVAAVLAACGTLRPPLEQRTTQGPTAAELWL
                     YRVGLANNRQPTFEESRHWEDDLDDRIAKYLRANPSAANSLDVSTFRFLRRAAVGQTK
                     EQIMILLGPPLATTSDPVEMEKVARKFWPDIKPRAQEAWMYPLGWYFYFGDSRVVDIT
                     QYQLR"
     gene            4465..>5717
                     /locus_tag="VLF19_12200"
     CDS             4465..>5717
                     /locus_tag="VLF19_12200"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSE94061.1"
                     /translation="MIRSQLVVTKRTPLAARGLVVAEHPMGAEVGAAVLARGGNAVDA
                     AVATAFAMTVVEPFMSTIAGSGTMLIHLADEGTTVALDFNAAAPLAAHAAMYTVVGGV
                     STALFPWPKVEGDANVSGHRAVAVPGSVAGLALALERWGTMTLADAIRPAIEIAEHGF
                     EPDWYQALSTAKFIEELSAYPASARNFLRNGRSIHRPPSLQPGDRVAYPDLARSLALI
                     AREGPDAFYRGALAQAIHDDMAAHGGLVTRDDLAAYRVQVAEPLRGRHRDLDLAFAPG
                     ATGGITALEVLNILGEFPKAAVRWSAPGGLHLRAEAIERAFADRFTHLGDPAHVKAPW
                     ERLASREHAREIAAEIRRGRPRRRARAGAASTDCTTHIGVVDRRRNMVALTHTAVSTF
                     GSFVVVPGTGILLNNGMIWFDPEPG"
BASE COUNT          758 a         2073 c         2078 g          808 t
ORIGIN      
        1 gcgcccaccc gcttctacaa gaccggcgtc gtcttcgccg cgtacctcaa cggccaccag
       61 cgccacttcc gcatggtcgg tggcctggag agcgcccgct cggtcgtgca cctcgcgcgg
      121 cagttcgtgc tgatggacca ggcggggctc ctgcgggacc cggaagcggc ggcggaacgg
      181 atgcggcgga cgctcgccgt cgccggcgtg gagtagcgcg gcccggccag cgccggattc
      241 gtctcgcggc gcagcagtcg gtccgtcgcg cagctcactc ccggtccccc tggccctcga
      301 gctggccctc ggccgcgccg aagagcacgt tgaacgtcgt gaggcttccg tagcaccggc
      361 tgatgtagct gcccacgccg aagcgcgggc gcgcgccgga cggcgtcgtg tcggtcatgg
      421 ggccgtcatt gtggcgcggc tcgcgcggcc atccgccgcc cgtgcccgcc gtcggcccgg
      481 gcggtcagag cacccagagc aggggtcggg cgcgcagcgt gtcggcggcc cagaaggcgg
      541 tgtcgcggaa ccacccgagc gcctcgtcgg cctcggctgt gcggcagagc gcgaacgtct
      601 caccgtcctc gaagaagccg ccgaaggcca gccgaccggc gccccgcagc tcgatgagcg
      661 cgaactccgc catgtcgggg tcggccacgc gcccctccac cacggtggcc cgccgcgagc
      721 cgtcgagcac gatctcggcc gcctgccacg gctccacgaa ctgcgagaag ctgcgcggcg
      781 tgtagcccgt ccacgcgccg gcggtccagt acgggtcctc ctcgatggcc ggctcgagct
      841 gcgccggctg ctggagccgg tacacgacgt cggcgccgcg gccgtcgggt gccgggccgc
      901 cgccgatgac agcgccgatg ctccggagcc cggccagccg ctcgatgtga gcgtgccggt
      961 gcggctcgcg gcgggcgacg tagtcctccg cgcacgtgaa cgccacgtgc acgacgatca
     1021 tgcggtcggc tcccggcggt gcgccctcag gattgccggc tctgcaagcg gatgccgaga
     1081 acgagcccgg accggctgtc gaccagacgt ccgagcccct gctgttgatg cgggctgatc
     1141 cgtgtcgagg gacggtcgat ctctccctgg gtccagatcg atcccaacgc gtgattcctc
     1201 ctgcctgccc ggggcaccgt accacggcca tcgtgaggcg gacaagcgcg aggagtctgt
     1261 gagcggcgag aggctaaatg cgcgatccat aggcccgcag cgtggccgcg ccgtcatccg
     1321 tgttaaggtg agcggagatg gcgctgctcg agcggttccg tgcgcgatac ccgttcccgc
     1381 tcgacgactt tcaggtcgag gcgtgccggg tcatcgagag cggcggctcg gtgatcgtct
     1441 cggcgcccac cggcgcgggc aagaccctgg tcgccgagtt cgccatccag atggcgctcg
     1501 agaccggccg ccggctcgcg tacacgacgc cgctcaaggc cctctccaac cagaagttcg
     1561 ccgatttcac gcgggccctt ggcgaggaca ccgtcggtat cctgacgggc gacgtcaagg
     1621 tgaatcccca gggccgcgtg ctcgtcatga ccacggagat cctgagaaac atgctctacg
     1681 gcggcggtct ggccgacctg ggctggatcg tcctcgacga gtgccactac atgggcgacg
     1741 aggggcgcgg cacggtgtgg gaggagatca tcgtcaacgc ccccgcggac gtgccgctgg
     1801 tggcgctgtc ggccacggtc gccaacgtgc gcgagatcgc cgactggatc tcgctggtcc
     1861 accggcccat cgccccgatc taccatcccc accggccggt gccgctctcc tacgggctcg
     1921 ccgacctcac cggcgagatc cacgagttcg acaaggtgcg cacgggccgg gcccgcgtca
     1981 tcggcgacga gcggccgggc gccgacgagc gcggccgctg gtactcgcgt cgcgtcgtgg
     2041 accccacggt gctcatcgag gcgctggaga cgcgcggatg gctgcccgcc atctacttca
     2101 tcttcagtcg cgccgggtgc gagcgcgcca tggacgacgt gctggtggac ggccgcgcgc
     2161 tgctctcccg cgagcagcag ggcgaggtgg accgcacgat cggcgaggcc ctccgcgaga
     2221 gccccacgat gggcgagtcg gagatcaacc agacgatctt ccgggccctg ggcctcggcg
     2281 tggccgtcca ccacgcgggc atcctgccga gcgtcaagcg gctcatcgag cggctcttcg
     2341 agcgtggcct ctgcaaggtg gtcttcgcca ccgagacgat gagcctgggc atccacatgc
     2401 cggcccgggc cgtcgtgctg cagggcctca ccaagcgcac cgagcgcggc ttccgctcgc
     2461 tcacccacaa cgagctcacc cagatggccg gccgcgccgg ccgccgcggc atcgacgccg
     2521 agggccagtg cgtgatcgcg ctcgatgcgc gcgacggcgt cgaggacgtc gtgcgcgtcg
     2581 tggacagcgc gccggagccg atcgccagcc agttcaagct cggctacggc tcggtggcgc
     2641 tgctgctggc gaccggcgcg gagcccgagg tgctgcgccg acgcatcgag gcctcgtttg
     2701 gtcagtacca gaacgtcaag cgcatccgcg agctggagga cggcctggcc cggctcgagg
     2761 cggcgctggc cgaggcccgg gcgttcgccg cgccgtgcgg cgacttcgag cgcatcgggc
     2821 gctatcgccg gctgcgccag gaggtcgacg cgcgccgacg ggccatgggc cggggtgcgc
     2881 gtcggggcga gcgcggcgtc atcgaggccg agccgggccg cctcgtgctg ttgcggcgca
     2941 agggcgggcc gagcctggcg atggtcctcg ccgtccacgg catccgcggt caccgcgcgc
     3001 tcctggaggc tctcctgccc cacggcgccg tcgtgcgcgt gaaggccggc gtcatcaagc
     3061 aggtcttctg gtcgacgccc acgctcgcgg tgccgcgcga cgcccacgcc ggcgcgcgcg
     3121 ggcgggaggg tcgcgggctg cgccacctgg ccgatcagct cgcacgcacg gacgtggctg
     3181 cgctgatcga gcgggagcga gcccaccgcc cggaggcctc gctcggcagc gtggagtgcc
     3241 atcggtgccc ctggggctcg acgccccatt gcgacgcggc gtggcgcgag gtcgagcgtc
     3301 tctccgagcg ggtggcgcag cggcgcggcg ccctcgacgc ggtccggggc gcgtactggc
     3361 aggagttcct gcgggtggtc gaggtgctcg agcagttcgg cgccgtccgg gggggctggc
     3421 tcgaggccaa ggggcgcctg gtcgccgggc tccgtcacga caacgagctg ctggtggcgg
     3481 aggtcgtgac gcgcgggatc ctgaccgatc tcacggtcgc ggaggccgcc gcggtgtgct
     3541 cggcgctcgg cgaggagtcg cgctcgggtg agccgatgat cgctcgtgcg ttcctccgct
     3601 cgcggccgaa gctccggcgc aagctcgagc acctcggcgc ggcggccgag gccgtcgccc
     3661 aggcccagcg ccagcgtcat ctcggcatgc cggtcgccgt gcatcccggc ttcatgcccg
     3721 cggtgttccg ctgggtctcg ggggacgacg actggtccgg gatcgtgcag cagtcgttcg
     3781 ggggccacga gggggacctg atccgggcga tgcggcggct catcgacctg ctccggcagc
     3841 tcggcgagag cgcggaggtg ccggccacga ccggccggtt gctgcttcag gccgcgcgcc
     3901 tggtggatcg cggcatcgtc ctggaatcgg cgctgatctg aggagcctct gctatgctga
     3961 tgaccgtgac gccccgggtc ctcggtctcg tgtgcgtcgc cgcggtgctc gcagcgtgcg
     4021 gcaccctgcg gccgccgctc gagcagcgca ccacgcaggg cccgaccgcc gcggagctgt
     4081 ggctctaccg tgtcggtctc gccaacaacc gccagcccac cttcgaagag agccggcact
     4141 gggaggacga cctcgacgat cgcatcgcca agtacctgag agcgaatccg agtgcggcca
     4201 acagcctcga cgtgtccacg ttccgcttcc tccgtcgcgc ggcggtcggt cagacgaagg
     4261 aacagatcat gatcctgctc ggcccgccgc tggcgacgac gtcggacccc gtcgagatgg
     4321 agaaggtcgc acggaagttc tggcccgaca tcaagccacg cgcgcaggag gcgtggatgt
     4381 acccgctcgg gtggtacttc tacttcggcg actcgagggt ggtcgacatc acccagtatc
     4441 agcttcgctg aggtgggaga ccggatgatc cgctcgcagc tcgtcgtgac caagcgcacg
     4501 ccgctcgccg cccgcggcct cgtggtcgcc gagcatccga tgggcgccga ggtgggcgcc
     4561 gcggtgctcg cccgcggcgg caacgccgtc gatgccgcgg tggcgacggc cttcgccatg
     4621 accgtcgtcg agcccttcat gagcacgatc gccgggtcgg gcacgatgct gatccacctc
     4681 gccgacgagg gcacgacggt cgcgctggac ttcaacgccg ccgcgccgct ggcggcgcac
     4741 gcggcgatgt acacggtggt cggcggcgtt tccacggccc tgttcccctg gcccaaggtc
     4801 gagggcgacg ccaacgtctc ggggcaccgt gcggtggcgg ttcccggatc ggtcgcggga
     4861 ctggccttgg cgctcgagcg ctggggcacg atgaccctcg ccgacgccat ccgcccggcg
     4921 atcgagatcg ccgagcacgg cttcgagccc gactggtacc aggcgctgag caccgcgaag
     4981 ttcatcgagg agctgtccgc ctatcccgcc agcgcccgga acttcctgcg caacggccgc
     5041 tcgatccacc ggccgccgtc gctgcagccc ggcgaccggg tggcctaccc cgacctcgcg
     5101 cgttcgctcg cgctcatcgc gcgcgagggg cccgacgcct tctaccgcgg cgcgctcgcg
     5161 caggccatcc acgacgacat ggccgcccac ggggggctcg tcaccaggga cgatctcgcc
     5221 gcctaccggg tgcaggtggc cgagccgctg cgcggacgcc accgcgacct cgatctggcc
     5281 ttcgcgcccg gcgccaccgg cggcatcacc gcgctcgagg tcctgaacat cctcggtgag
     5341 ttcccgaagg ccgcggtgcg ctggtcggcg ccgggcggcc tgcacctgcg cgccgaagcg
     5401 atcgagcgcg ccttcgccga tcgcttcacc cacctcggcg atccggcgca cgtgaaggcc
     5461 ccctgggagc ggctggcgtc gcgggagcac gcacgggaga tcgccgccga gatccgccgg
     5521 ggccggccgc ggcgtcgcgc gcgggccggg gcggcgtcca cggactgcac gacgcacatc
     5581 ggagtcgtcg accgccggcg caacatggtg gcgctcacgc acaccgccgt ctcgacgttc
     5641 ggatccttcg tcgtcgtgcc ggggacgggg atcctgctga acaacggcat gatctggttc
     5701 gatcccgagc cgggcaa
//
LOCUS       DATIAZ010000358         4215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1562280, whole genome shotgun sequence.
ACCESSION   DATIAZ010000358 DATIAZ010000000
VERSION     DATIAZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562280"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..1159)
                     /locus_tag="VLF19_12205"
     CDS             complement(416..1159)
                     /locus_tag="VLF19_12205"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSE94062.1"
                     /translation="MPIPPDALIFVAVVIFAGYLIFGVTGFGASPITIPVLVHVLPLT
                     FVLPLAAVLDLGSALALTLHTRRQADSRELLTLVPFTLVGLTLGVTLLVSLPRTATLL
                     ALGSFVCVYALHVMLRRETVRRLTRWWAAPAGIAGGVLGALFGMGGPPYVAYIMGRVP
                     NPAAQRATISQMVILNVGLRVVVFAIAGLLGSPSFWTAVACLLPVAWAGVWVGNRVHV
                     RVASTTAARIIGAALFLVGAALIVRTFQS"
     gene            1270..2235
                     /locus_tag="VLF19_12210"
     CDS             1270..2235
                     /locus_tag="VLF19_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HSE94063.1"
                     /translation="MHSVLIALVAAALVLLPAAARSAEVVIQTYVLPPGGGPHDVAVD
                     AAGIAWYTAQRSGHLGRLDPKTGKVDLVPLGAGAHPHGVIIGPDGGAWITESGTNAIV
                     RVDPATREVKRWPLPRARGYVNLNTATFDKRGRVWFTGQNGIYGRLDPTTGEMQVWDA
                     PRGRGPYGITTTPSGDIYYASLAGSHIARIDVETGTASVIEPPTKNQGARRVWSDSKG
                     RIWVSEWNSGNVSCYDPQTRQWRTWRLPGDTPRAYSVYVDHDDKVWLSDFGANAMVRF
                     DPETEKFQVFPHERSGAGVRQMLGRPGEVWAPESGTNRLVVYRTR"
     gene            complement(2412..3080)
                     /locus_tag="VLF19_12215"
     CDS             complement(2412..3080)
                     /locus_tag="VLF19_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94064.1"
                     /translation="MSRQREFLADADAVLLTRDPTGLALALVKVGAASSAANVSRAAA
                     HLFLVEPLLPATGWWDRAGASHPPIEERVAVLGRMGSGIPPEAVDAAREAGVRFRTAP
                     DERAAPAGASVVAHRPTPARREADDVTPGPSPAGALAGRDDGLPAAGTAGGSSVVSYL
                     RLAQGPVQLLAQPHTAAGPCEALAAGTLLALLAVEGEFLRVQTTSGVTGYIGKDTAVT
                     WSSD"
     gene            complement(3459..>4215)
                     /locus_tag="VLF19_12220"
     CDS             complement(3459..>4215)
                     /locus_tag="VLF19_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSE94065.1"
                     /translation="ELIWARTLGHLIFVVALFAPARGGWRLLGTQRPAIQVARSLLLL
                     VSTSFFFSAIGHVPLADATAVSFTSPFIVAAVAGRVLGERVTREHWISIAIGFAGALI
                     VIRPTGGGTHPAIVLVLGSAACYAFYQVLTRRVVGIDSPETSVTYSALVGTLVLSAVV
                     PFVWRTPAGAGQWLMLAGLGLLGGLGHYFVARALLWGPASVIAPFHYVQLVWAAAAGF
                     LVFGDVPSAWTWLGAAIVIGSGLSLAWRESRRP"
BASE COUNT          734 a         1514 c         1473 g          494 t
ORIGIN      
        1 gtagatgcgc gaaacccccg cctgggagca ggcgatctcg cgcatcgcac aaggccgcga
       61 actcgcgtag ttttcgccac ccggaaggct cgggaagact tgacgaccgg accgacggcg
      121 tggccggttc agctggccaa cacggccgca agcacggacc acgctcgctt caggccggcc
      181 gagcagccgg ggaatcggac ggccgacggc ggccggcaac tccacgccga ggagccgggc
      241 gaatcctttc gtggccggca ccatcgtcgg gggcctggcg cggcggctgg ccgaacgcca
      301 cgacaaaccg ctcccgcaac ggtcagggcg aagatcagcg cggggtcagg gcgaccctcg
      361 cgccggagcg cgttcacccc agcgcggcgg atcacgtgtg acgtgcttcg ccaggtcaag
      421 actggaatgt ccgcacgatc agcgcggcgc cgacgagaaa cagtgccgcg ccgatgatgc
      481 gagccgccgt ggtggatgcc acgcgcacgt gcacgcgatt gccgacccac acgccggccc
      541 atgccaccgg gagcaagcac gctaccgccg tccagaacga aggtgagccg agaagtccgg
      601 cgatggcgaa caccacgacg cgtaacccga cgttcaggat caccatctgc gagatcgtcg
      661 cacgctgagc cgccgggttg gggacccggc ccatgatgta cgcgacgtac ggggggccgc
      721 ccatgccgaa cagggcgccc agcacgccac cggcgatgcc cgcgggcgcg gcccaccacc
      781 gggtcaaccg ccgcaccgtc tcccggcgga gcatcacgtg gagcgcgtac acgcacacga
      841 acgaccccag cgccagcagc gtggcggtgc gtggcaggct gacgagaagc gtcacgccca
      901 acgtgagccc gacgagcgtg aaaggcacga gcgtcaggag ctcgcggctg tccgcctgcc
      961 ggcgcgtgtg cagcgtcagc gccagcgccg agccaagatc gaggaccgcg gcgagcggga
     1021 gcacgaacgt gagcggaagg acgtggacca ggacgggaat cgtgatcggc gaggcgccga
     1081 agcccgtgac gccgaatatc aggtacccgg caaagatcac gaccgccacg aagatcagcg
     1141 cgtcaggagg gatcggcacg cgttcttccg gtccctgggg acgtcctcgc ctgctgaccg
     1201 ttcaccggtc ccggcctcga gggcttggtc cgcgcgcgat aatgtccgcc aatgacaggg
     1261 gaggtgccca tgcacagcgt cctgatcgcg ctcgtcgccg ccgccctcgt cctgctcccg
     1321 gccgcggcac ggtcggccga ggtcgtcatc cagacctacg tgctgccgcc cggcgggggg
     1381 ccgcacgacg tcgccgtcga cgccgccggc atcgcctggt acacggccca gcgcagcggc
     1441 cacctcggcc gcctcgaccc gaagaccggc aaggtggacc tcgtgccgct gggggccggc
     1501 gcgcacccgc acggcgtcat catcgggccc gacggggggg cctggatcac ggagagcggc
     1561 accaacgcga tcgtgcgggt cgacccggcg acgcgcgagg tgaagagatg gcccctgcct
     1621 cgggcgcgcg gctacgtgaa cctcaacacc gcgaccttcg acaagcgcgg ccgtgtgtgg
     1681 ttcacgggcc agaacgggat ctacggccgg ctggacccga cgacgggtga gatgcaggtg
     1741 tgggatgcgc cgcgcggccg cggcccctac gggatcacca cgacgccgag cggagacatc
     1801 tactacgcct ctctcgccgg cagccacatc gcccgcatcg acgtcgagac gggcaccgcc
     1861 agcgtcatcg agccgcccac gaagaaccag ggcgcgcggc gggtgtggtc ggactcgaag
     1921 ggcaggatct gggtgagcga gtggaactcc ggtaacgtga gctgctacga cccgcagacc
     1981 cgccagtggc gcacgtggag gctgcccggc gacacgccac gcgcctactc cgtctacgtc
     2041 gaccacgacg acaaggtgtg gctcagcgac ttcggcgcga atgccatggt ccgcttcgat
     2101 ccggagaccg agaagttcca ggtcttcccg cacgaacgga gcggcgccgg ggtgcgccag
     2161 atgctgggac ggcccggcga ggtctgggcg ccagagtccg gcacgaatcg cctcgtcgtc
     2221 tatcgcacga ggtgagaccg tgaaacgtcg cctggatccg gcccgtcgcg ttcatcttcg
     2281 tccggtccgc gttcggttca gtgagagatc acgtcgagga tggcgaggtt gacgacgagc
     2341 gccgccgcca cgaccgcccg gaccgcgcca agcatcaccg cccgctcaag catgacgcct
     2401 cctggctgtc ttcagtcgga gctccaagtc actgccgtgt ccttcccgat gtagcccgtg
     2461 accccgctcg tggtctgcac gcggaggaac tcgccctcga cggccagcag tgccagcaga
     2521 gtgcccgccg cgagcgcctc gcacggcccg gccgcagtat ggggctgcgc gagcaactgg
     2581 accggccctt gcgccaaccg caggtacgat acgacgctgg agccgcccgc agtacccgcg
     2641 gcgggcagcc cgtcgtcgcg ccccgccagg gcgcccgccg gcgacggccc gggagtgaca
     2701 tcgtcagctt cccgtcgagc cggtgtcgga cggtgagcga ccacggacgc accagccggc
     2761 gccgcccgtt cgtccggggc tgtacggaag cgcacgcccg cctcgcgcgc tgcatcgacg
     2821 gcctcgggcg gaatcccgct tcccatccgg ccgagcaccg cgacacgctc ctcgatcgga
     2881 ggatgcgacg ccccggcacg gtcccaccag cccgtagccg gaagcagcgg ctccacgagg
     2941 aagaggtgcg cggccgctct cgacacgttg gcggcgctgc tcgccgctcc gaccttcacg
     3001 agggcgagcg cgagccccgt gggatcccgg gtgagcaaga cggcgtcggc gtcggccagg
     3061 aactcgcgct gccgggacac ggctccgccg acgcgtcgtc cacagagctg agccccgaag
     3121 aggacataga acggagatcc catgaccagc ggccctcgat ccccgaccag gcctcgcgta
     3181 cgcggttccg tgtgcgcacg agcttgttgt cgacattgac cacccaggcc aagacgagga
     3241 ccacgacgat caggacgatc cagagcgtga catcggatgc gcccatgtcc actcttcctc
     3301 tccgacaccg gatggcgctg cgcttcgcat agcatgatag cgtcagtcgt cttcgccgat
     3361 ccagtgagtg tcgtgaggcg agatcggtcc tgccctggca gaggccgagg cggcgcgccc
     3421 gacgctcggc atccacggca cggtccgctc ggtgcgactc acggtctccg gctctcccgc
     3481 cacgcgagag agaggccgct gccgatgacg atggccgcgc cgagccaggt ccacgcggac
     3541 ggcacgtcgc cgaacacgag gaacccggcc gcggcggccc acacgagctg cacgtagtgg
     3601 aagggcgcga tcaccgaggc tggcccccac agcagcgccc gcgccacgaa gtagtgaccg
     3661 aggccgccga gcaggccgag gcccgccagc atcagccact ggccggcgcc ggccggcgtc
     3721 cgccagacga agggcaccac ggccgacagg acgagcgtgc ccaccagcgc gctgtaggtg
     3781 acgctcgtct cgggcgaatc gatgccgacc acccgacgcg tgaggacctg gtagaacgcg
     3841 tagcaggcgg cactgcccag caccaggacg atcgcggggt gcgtgccccc gccggtcggc
     3901 cggatcacga tgagcgcccc cgcgaagccg atggcgatcg agatccagtg ctcgcgcgtg
     3961 acgcgctcgc ccagcacgcg gccggcgacg gcggccacga tgaagggcga ggtgaacgac
     4021 acggcggtgg cgtcggcgag cggcacgtgc ccgatcgccg agaagaagaa cgacgtggag
     4081 acgagcagca gcaaggatcg cgcgacctgg atcgccggcc gctgcgtgcc gagcaggcgc
     4141 cagccgccgc gcgccggagc gaagagcgcg accacgaaga tcaggtggcc cagcgtgcgc
     4201 gcccagatca gctcg
//
LOCUS       DATIAZ010000359         8857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1035453, whole genome shotgun sequence.
ACCESSION   DATIAZ010000359 DATIAZ010000000
VERSION     DATIAZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035453"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="dapF"
                     /locus_tag="VLF19_12225"
     CDS             complement(<1..791)
                     /gene="dapF"
                     /locus_tag="VLF19_12225"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSE94066.1"
                     /translation="MIPFAKGHGLGNDYLVMEQRDLPASLSARAIARICDRNRGVGSD
                     GILLLVPSARADFGLRIFNPDGSEAEKSGNGLRIFAKYLWDHGHAKQATFTVDTKGGV
                     VECQLHVRDGHVNFVTVEMGRATFHAAEVPMTGPAGEAVGVALALRDGAALTVTAVSV
                     GNPHCVVFVDRLDEGETRRLGPQLETHAAFPNRINVQFARVVARDALEILIWERGAGF
                     TLASGSSSCGAAAAAVRTGRCDHGRVAVRMPGGELAVHVRPDWSLR"
     gene            complement(788..1948)
                     /locus_tag="VLF19_12230"
     CDS             complement(788..1948)
                     /locus_tag="VLF19_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSE94067.1"
                     /translation="MSRPLRVGIVGARFAAELHAANYRHLRDRAALTAVCARSRESAE
                     AFAGRHGIAAVFTDYRALCESPQVDAVDVCATTDVHHEVVVAAARAGKHVIVEKPLTG
                     WFGDGSDSRAAMLEGALRNCDAVLEAVAGAGVILCYAEDFVYAPPVAKLRRLLDASGG
                     AILELRAEESHSGSHATYARRWRTSGGGSLLRMGSHPIGVVLQLKHHEGRVRSGRPIR
                     ARSVVADVARLTALPAVTRGGFLRAAPEDVEDWAVAVVTFEDGTKATVHSSDVTLGGV
                     RNVVTAYLTSGVVQANITPNTSVVAYAPEDRVWGDEYITEKVETRAGWQFPSPEEDWM
                     RGYPQELEDFVDAIAAKREPLSGGALAREVVEVIYAGYLSAETGRRVDLHRP"
     gene            complement(1973..3100)
                     /locus_tag="VLF19_12235"
     CDS             complement(1973..3100)
                     /locus_tag="VLF19_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSE94068.1"
                     /translation="MGSFKEGLEDVVVSTSEICFIDGREGRLLYRGYDVDDLVQQSSF
                     EEVVYLLWHGALPNRKERDAHVKALASTANRRLHPKLITMLRQLPRKTTPMEVLRTGV
                     SALSAFDPDAGDNSREATVRKAIRVTAQMPTLVAAWERLRRGKAPVAPSAKLSLAANF
                     LYMLTGKKPTDLAARTFDVALILHADHEFNASTFAARVTAATLSDIHSAVVSGIGALK
                     GPLHGGANEQVMLMVEQIKTPERAEAWIRRALTDKARVMGFGHRVYRVEDPRAKHLRR
                     LATELGEQTGKPQYVQILNTVARVVSEDKKIYPNVDLYSGAAYAAMGIPTDQFTPIFA
                     ISRVVGWAAHVLEQHAHNRLIRPRAEYTGPTRKTYVPIDQR"
     gene            3176..4003
                     /locus_tag="VLF19_12240"
     CDS             3176..4003
                     /locus_tag="VLF19_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSE94069.1"
                     /translation="MRVIDFYDRHPISEAQVLAAVARRRGGDALADPLGAEDLFAFDQ
                     DHYGGLAAVAALARRAGIDAASHVLDVCAGLGGPARFLAARLGCRVVALELHAGRAAG
                     AARLTRLVRLDERVAVVRGDATALPLATGRLDACVSQEALLHVSDKAAVLRESHRVLR
                     PGGRLAFTDWIAQPRLGDGERRRLEQWMAATTLQGLDGYRQLLGAAGFTGIEAEDLTD
                     EWRPILRARLEMYRALREDTAARFGEAWYREYQQVYGFFIGLVEAGKLGGGRFTATR"
     gene            complement(4007..5635)
                     /locus_tag="VLF19_12245"
     CDS             complement(4007..5635)
                     /locus_tag="VLF19_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HSE94070.1"
                     /translation="MSRVVRVVLIAVAAVVVLVVAALVAVPYLVDTPRVQALIASTAT
                     QTLGRPVKFASLSVSVLPLPAVVLGNLEVAEDPAFGKGPFLKLREMRVRLKLWPLLFL
                     RVELGDFLLEKPAISLVQAADGRWNVATLGGHAPESGSPPSRPRGGGGGGAAGGAALL
                     GSRVKIEDGVVTYEARGPGAPARYHVEDLDLTVTGSPTGLAFRGNARVEPGALAVRIA
                     EGLVGVNGARGLMEAPVRGQVSLDGKSLGALAAVAMGPEPALAGGLTGRLTLDGTVGK
                     PRATGDVELGDFAVSQTVAQCPEPKRRTLALGTVKSKVAWEDPRFAARPLTTSLGGGS
                     ITANVTATLDRGVRVELGDIGIKGVPAEKVLVDFLCQGYAVSGPLDLTGSASARTSDL
                     WNTLDGKGQLRVGPGKIVGSQALALLENVVRVGGAVSALLGENLPRGLRTSGLEYDSI
                     TGTFTITNGVLSTRDLKLASRTLTASAAGTYGLANGAVNMDIGLSSGQTQLQAKVTGS
                     ADRPSIRVNPTATLRDIDPAKVEKGLQDLLKRFR"
     gene            complement(5658..6587)
                     /locus_tag="VLF19_12250"
     CDS             complement(5658..6587)
                     /locus_tag="VLF19_12250"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HSE94071.1"
                     /translation="MKRSTWIAFLGLLAMTALAAATIDAEARARSGGSRGSRTYSAPV
                     RPDSTSPTAPSRTATAPSAPAPQRPGLLGGFGGMLGGLLLGGLLGGLLFGNHGFGIGL
                     LDVLLIGGGLALLMTVLRRRREEPRPAYAMAGGGGPYDRAPAADWQGGVATVAAPAAP
                     SDLERGIGHIRTLDARFDPGAFAEVARTAFLDVQRAVGARDVAPLRERLTPEMFAVLQ
                     GQVDEVRAARRTSHVEDVRFDRVDVSEAWQESGRDFVTVYLTGSLLDYTVSDTTREVV
                     NGSRTAPQAFEEFWTFTRPVGPNGWRLSAIQAT"
     gene            complement(6643..7611)
                     /locus_tag="VLF19_12255"
     CDS             complement(6643..7611)
                     /locus_tag="VLF19_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HSE94072.1"
                     /translation="MKAVRVHTPGGPDVLRHEDVPQPTPKAGEAIVKLDAAGLNYIDV
                     YFRTGLYKAEVPFTPGMEGGGTVAAVGAGVTDVKVGDKVAYTGVAGAYAEFAAVPAAR
                     LVALPAGVTTKQGAAAMLQGLTAHYLACSTYPLKAGDTCLVHAAAGGVGLLLVQMAKM
                     RGARVIGTVSTPEKAKLAREAGADETILYTQQDFEAEVKRMTGGKGVQVVYDGVGKTT
                     WDQSLNSLAPRGTMALYGASSGPIGQIDPQILNAKGSLFLTRPSLNHHIATREELLQR
                     SGDVLGWIRDGKLQVRMEFAFPLKDAAEAHRALEGRKTTGKVLLLP"
     gene            complement(7656..8549)
                     /locus_tag="VLF19_12260"
     CDS             complement(7656..8549)
                     /locus_tag="VLF19_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94073.1"
                     /translation="MARHPARPASRLVPAHGLAGIGIIVAAELLLFGGNRIVGEWFTP
                     VVWTGYVLLLDAVVARLTGRSYLTTGRLELLLVALASIAAWWLFELYNAPRFWRGGDA
                     ARGLWWQYHRLEPDLFLRRVGYDWAFATILPALFLTAAALRATVFARVRVRPWAPTPA
                     LARASVAVGAAAVALPLLVVSAWLVPLVWLGFILLLEPLNARAGRPSWLADLARGDAS
                     RLCALLAAGAACGVLWEFWNYWAATRWTYTVPYLGNVKVFEMPVLGYLGFPPFALECH
                     AMYHWARARLLPDDGVPAPLI"
     gene            complement(8576..>8857)
                     /locus_tag="VLF19_12265"
     CDS             complement(8576..>8857)
                     /locus_tag="VLF19_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94074.1"
                     /translation="RDRLMLWVELHGDKEDFARFLAPRLSVGDRIIQPSIVQNERTAI
                     RGEGGRYLARCVYWFPAKDVDGDSRVTLLIQTPEGQERTRFEIDLAKMR"
BASE COUNT         1234 a         3233 c         3176 g         1214 t
ORIGIN      
        1 cgcagcgacc agtccgggcg cacgtgcacg gccagctcgc cgcccggcat gcggacggcg
       61 acgcgcccgt ggtcgcaccg gccggtacgc acggccgccg cagccgcccc acacgaggac
      121 gagccggagg cgagcgtgaa gccggcgccg cgctcccaga tgaggatctc gagggcgtcg
      181 cgggccacga cgcgggcgaa ctgcacgttg atgcgattgg ggaaggccgc gtgggtctcg
      241 agctgcggtc cgagccgccg cgtctcgccc tcgtcgagac ggtccacgaa gaccacgcag
      301 tgcgggttgc cgacggagac ggccgtcacc gtcagcgccg cgccgtcgcg cagcgcgagt
      361 gccacgccga cggcctcgcc ggccggccct gtcatgggca cctcggcggc gtggaaggtg
      421 gcccggccca tctcgacggt gacgaagttc acgtggccgt cgcggacgtg gagctggcac
      481 tccaccaccc cgcccttcgt gtccaccgtg aacgtggcct gcttcgcgtg gccgtggtcc
      541 cagaggtact tggcgaagat gcgcaggccg ttgcccgatt tctcggcctc gctgccgtcg
      601 ggattgaaga tacgcaggcc gaagtcggcg cgcgcgctcg gcaccagcag gaggatgccg
      661 tcggagccca caccccggtt ccggtcgcag atccgcgcga tcgcgcgtgc cgagagcgaa
      721 gccggcaggt ctcgctgctc catcaccaga tagtcgttgc cgaggccgtg gcccttcgcg
      781 aacgggatca cgggcggtgc aggtccacgc gccggcccgt ctccgcggag aggtagccgg
      841 cgtagatgac ctcgaccacc tcgcgagcga gcgcgccgcc ggagagcggc tcgcgcttcg
      901 ccgcgatcgc gtccacgaag tcctccagct cctgggggta gccgcgcatc cagtcctcct
      961 caggactcgg gaactgccag cccgcccgcg tctcgacctt ctcggtgatg tactcgtcgc
     1021 cccacacgcg gtcctcgggc gcgtaggcca ccacgctcgt gttgggcgtg atgttcgcct
     1081 gcacgacgcc gctggtgagg taggcggtga cgacgttgcg caccccgccg agggtcacgt
     1141 cgctggagtg cacggtggcc ttggtgccgt cctcgaacgt cacgaccgcc accgcccagt
     1201 cctccacgtc ctccggcgcc gcgcgcagga agccgccgcg ggtcacggcc ggcagcgcgg
     1261 tgagccgcgc gacgtcggcg acgaccgagc gcgcgcggat cgggcggccg ctgcgcacgc
     1321 gcccctcgtg gtgcttgagc tgcaggacca cgccgatcgg gtgcgagccc atccggagca
     1381 gcgagccgcc gcccgacgtg cgccagcggc gcgcgtaggt ggcgtgggac cccgagtggc
     1441 tctcctcggc gcgcagctcc aggatggcgc cgccgctggc gtcgagcagg cgccgcagct
     1501 tggccaccgg cggcgcgtac acgaagtcct cggcgtagca gaggatcacg ccggccccgg
     1561 ccacggcctc gagcaccgca tcgcagttgc ggagcgcgcc ctccagcatg gccgcgcggg
     1621 agtcgctgcc gtccccgaac cagcccgtga ggggcttctc cacgatgacg tgcttgccgg
     1681 cccgcgcggc ggcgacgacc acctcgtgat gcacgtcggt ggtcgcgcac acgtccacgg
     1741 cgtcgacctg gggcgactcg caaagcgcgc ggtaatcggt gaagacggcg gcgatgccgt
     1801 gccgcccggc gaacgcctcg gcggattcgc ggcttcgcgc gcagacggcg gtgagggccg
     1861 cgcggtcgcg gagatggcgg tagttggcgg cgtgcagctc cgcggcgaag cgtgcaccca
     1921 cgatcccgac ccgcagcggc cggctcacgg cagcgcccgc cggcggcgac cgctaccgct
     1981 gatcgatcgg gacgtacgtc ttgcgcgtcg gcccggtgta ctcggcccgc ggccggatga
     2041 gccggttgtg ggcgtgctgc tcgagcacgt gcgcggccca gcccacgacg cggctgatgg
     2101 cgaagatcgg cgtgaactga tcggtcggga tccccatcgc cgcgtaggcg gcgccggaat
     2161 agagatcgac gttggggtag atcttcttgt cctcgctcac cacgcgcgcc accgtgttga
     2221 ggatctgcac gtactggggc ttgccggtct gctcgcccag ctctgtggcc aggcggcgca
     2281 ggtgcttggc gcgcggatcc tcgacccggt agacgcggtg gccgaagccc atcacgcggg
     2341 ccttgtcggt cagggccctg cggatccacg cctccgcccg ctccggtgtc ttgatctgct
     2401 ccaccatgag catcacctgc tcgttggcgc cgccgtggag cggccccttc agcgcgccga
     2461 tgcccgagac cacggccgag tggatgtcgg agagcgtcgc cgccgtcacc cgcgcggcga
     2521 aggtggaggc gttgaactcg tggtcggcgt gcaggatgag cgcgacgtcg aacgttctgg
     2581 cggcgaggtc ggtcggcttc ttcccggtga gcatgtagag gaagttggcg gcgaggctca
     2641 gcttcgcgct gggcgccacc ggcgccttgc cgcggcgcag tcgctcccac gccgccacca
     2701 gcgtgggcat ctgggcggtg acgcggatcg ccttgcgcac ggtcgcctcg cgggagttgt
     2761 cccccgcgtc cgggtcgaac gcggagagcg cggagactcc ggtgcgcagc acctccatcg
     2821 gcgtcgtctt cctcgggagc tgacggagca tcgtgatcag cttcgggtgg agccggcgat
     2881 tggcggtcga ggcgagagcc ttgacgtggg cgtcacgctc cttgcggttc ggcagggcac
     2941 cgtgccacag gagatagacg acctcctcga aggaggactg ctgcacgagg tcgtccacgt
     3001 cgtagccgcg gtagagcagg cggccttcgc ggccgtcgat gaagcatatt tccgacgtgg
     3061 agacgacgac gtcctcgagg ccttccttga agctgcccat ggttctccct cacgcggggc
     3121 cttgaaacgg tttgcttttt atcacaggac gccggaccct tcaaggcacg ccaccgtgcg
     3181 cgtcatcgac ttctacgacc ggcatccgat cagcgaggcc caggtcctgg ccgccgtcgc
     3241 gcgccggcgc ggcggcgacg ccctggctga ccccctcggc gccgaggacc tcttcgcctt
     3301 cgaccaggac cactacgggg gcctggccgc cgtcgccgcg ctcgcgcgcc gcgccgggat
     3361 cgacgccgcc tcgcacgtgc tggacgtgtg cgccggcctc ggaggccccg cgcgcttcct
     3421 ggcggcgcgt ctgggctgcc gggtggtcgc cctcgagctc cacgccggcc gggccgccgg
     3481 cgccgcccgc cttacgcgtc tggtccgtct ggacgagcgc gtggcggtcg tgcgcggcga
     3541 cgccacggcg ctcccgctcg cgaccggccg cctcgatgcc tgcgtgagcc aggaggcgct
     3601 gctccacgtt tcggacaagg ccgcggtgct gcgcgagtcc catcgggtgt tgcgtcccgg
     3661 cgggcggctc gccttcaccg actggatcgc acagccacgg ctcggcgatg gcgagcgccg
     3721 ccggctcgag cagtggatgg cggccacgac gctccagggc ctcgacggct accggcagct
     3781 gctcggcgcc gccggcttca ccggcatcga ggccgaggac ctcaccgacg agtggcggcc
     3841 catcttgcgg gcccgcctcg agatgtaccg cgcgctccgc gaggacaccg ccgcgcgctt
     3901 cggcgaggcg tggtaccgcg agtaccagca ggtctacggc ttcttcatcg ggctggtcga
     3961 ggccggcaag ctcggcggcg gccgcttcac cgcgacccgc tgagcctcag cggaaccgct
     4021 tgagcaggtc ctgcaacccc ttctcgacct tggcgggatc gatgtcgcgg agggtcgccg
     4081 tcgggttgac gcggatcgac gggcgatccg cggagcccgt cactttcgcc tggagctgcg
     4141 tctggcccga ggacagcccg atgtccatgt tcacggcgcc gttcgccagc ccgtaagtgc
     4201 cggccgcgct ggcggtgagc gtccggctcg ccagcttgag atcgcgggtg ctgagcacgc
     4261 cgttggtgat cgtgaacgtg cccgtgatgg agtcgtattc gaggcccgac gtgcggaggc
     4321 ccctgggcag gttttcgccc aggagggcgg acacggcacc gccgaccctc acgacgttct
     4381 cgagcagcgc gagcgcctgg gagccgacga tcttgccggg accgaccctc agctgcccct
     4441 tgccgtcgag cgtgttccag agatcgctcg tgcgagccga ggcgctgccg gtcaggtcga
     4501 gcgggcccga gaccgcatag ccctggcaga gaaagtccac gaggaccttc tcggccggca
     4561 cgcccttgat gccgatgtcg ccgagctcca cgcgcacgcc acgatcgagc gtcgccgtca
     4621 cgttggcggt gatgctgccg ccgcccaggc tcgtcgtcag gggtcgcgcc gcgaagcgcg
     4681 ggtcctccca cgcgaccttc gatttcaccg tcccgagcgc gagggtgcgc cgcttgggct
     4741 cggggcactg cgcgaccgtc tggctcaccg cgaaatcgcc gagctcgacg tcaccggtcg
     4801 ccctgggctt cccgacggtg ccgtcgagcg tgaggcggcc cgtgaggccg ccggcgagcg
     4861 cgggctcggg acccatcgcc acggctgcca gggcgccgag gctcttgccg tcgagcgaga
     4921 cctgaccgcg gacgggcgcc tccatgaggc cgcgcgcgcc gttcacgccc acgagcccct
     4981 cggcgatccg cacggcgagc gcgcccggct ccacgcgcgc gttcccgcgg aacgcgagcc
     5041 ccgtggggct cccggtcacc gtcaggtcga ggtcctccac gtgatagcgc gccggcgcac
     5101 cgggcccgcg agcctcgtag gttacgacgc cgtcctcgat cttcacgcgt gagcccagga
     5161 gcgcagcccc cccggcagcg ccgcctccgc caccgccgcg tggccgggag ggcggactgc
     5221 cgctctccgg tgcgtggccg ccgagcgtcg ccacgttcca gcggccgtcg gcggcctgca
     5281 cgagcgagat cgccggcttc tcgaggagga agtcgcccag ctccaccctc aggaacagca
     5341 gcggccagag cttgagccgg accctcatct cgcggagctt gaggaacggg cccttgccga
     5401 acgccgggtc ctcggcgacc tcgagattcc cgagaaccac cgccggcagc ggcagcacgg
     5461 acacggacag cgaggcgaac ttgacgggac gtcccagcgt ctgcgtggcc gtgctcgcga
     5521 tgagcgcctg gacccgcggt gtgtccacga ggtagggcac cgcgacgaga gccgccacca
     5581 cgagcacgac gacggccgcg acggcgatga gaacgacgcg gaccacccgg ctcatcgcaa
     5641 accgccgccg cgtggtccta cgtcgcctgg atcgccgaca gtcgccagcc gttcggcccc
     5701 acgggccggg tgaacgtcca gaactcctcg aacgcctgcg gcgcggtgcg cgagccgttc
     5761 acgacctcgc gcgtggtgtc gctcaccgtg tagtcgagca gcgagcccgt gaggtagacg
     5821 gtcacgaagt cgcggccgct ctcctgccac gcctcgctga cgtccacccg gtcgaagcgg
     5881 acgtcctcga cgtggctcgt ccgccgggcg gcacgcacct cgtcgacctg cccctgcagc
     5941 accgcgaaca tctccggggt gaggcgctcg cgcagcgggg ccacgtcgcg cgcgcccacg
     6001 gcacgctgca cgtccaggaa ggccgtgcgc gccacctcgg cgaaggcgcc ggggtcgaac
     6061 cgcgcatcca gtgtgcggat gtgaccgatg ccccgctcca ggtcggaggg cgccgccggc
     6121 gccgccacgg tggccacgcc gccctgccag tcggcggccg gcgcgcggtc gtacggaccg
     6181 ccgccgccgg ccatcgcgta cgccggccgg ggctcctccc gtcgccgccg caggaccgtc
     6241 atcagcagcg cgaggccgcc gccgatcagc agcacgtcga gcaggccgat cccgaagccg
     6301 tggttcccga agagcaagcc cccgagcagc ccaccgagga gcagaccgcc gagcatgccc
     6361 ccgaagccgc ccaagagccc gggccgctga ggcgccggtg ccgacggcgc ggtcgccgta
     6421 cgactcgggg cggtcggtga cgtcgagtcc gggcgcaccg gcgcggagta cgtgcgcgag
     6481 ccgcggctgc cgccgcttcg cgcccgcgcc tcggcgtcga tcgtggcggc ggcgagcgcg
     6541 gtcatggcca acagcccgag aaacgcgatc cacgtgctgc gcttcatgcg tcacctcccg
     6601 tcgtcggggc cattgtatcg caccgtccgc cggccgcccc gctcagggca gcaacagcac
     6661 cttgcccgtg gtcttgcgcc cctcgagggc gcgatgggcc tcggcggcgt ccttgagcgg
     6721 aaacgcgaac tccatccgta cctggagctt gccgtcccgg atccagccga gcacgtcgcc
     6781 cgaccgctgc agcagctcct cgcgcgtcgc gatgtggtgg ttgaggctcg ggcgcgtgag
     6841 gaagagcgag cccttggcgt tgaggatctg cgggtcgatc tggccgatcg gccccgagga
     6901 cgcgccatag agcgccatgg tgccgcgcgg ggcgagcgag ttcaggctct ggtcccacgt
     6961 ggtcttgccg acgccgtcgt agacgacctg cacgcccttg ccgccggtca tccgcttgac
     7021 ctcggcctcg aagtcctgct gcgtgtagag gatggtctcg tcggcgcccg cctcgcgcgc
     7081 gagcttggcc ttttccggcg tggacacggt gccgatcacg cgcgcgcccc gcatcttcgc
     7141 catctggacc agcaagagac cgacgccgcc ggccgcggcg tggacgagac aggtgtcgcc
     7201 ggccttcagc ggataggtcg agcacgccag atagtgcgcg gtcaatccct gcagcatcgc
     7261 ggcggcgccc tgcttcgtcg tcacgcccgc gggcagcgcg acgagccgcg cggccggcac
     7321 cgccgcgaac tccgcgtagg ctccggcgac gccggtgtag gcgaccttgt cgccgacctt
     7381 cacgtcggtc acgccggcgc cgacggcggc gacggtgccg ccgccttcca tgccgggcgt
     7441 gaacggcacc tcggccttgt agagaccggt gcggaagtac acgtcgatgt agttgaggcc
     7501 ggcggcgtcg agcttcacga tcgcttcgcc cgccttgggg gtgggctggg ggacgtcctc
     7561 gtggcgcagc acatcgggcc cgccaggcgt gtggacgcga acggctttca tcacgtgtcc
     7621 tcctgatgtg gcgattcggt gagacggcga gcggattata tcaggggcgc cggaacgccg
     7681 tcgtccggca ggagccgggc ccgggcccag tggtacatcg catggcactc gagcgcgaac
     7741 ggcggaaagc ccaggtagcc gagcaccggc atctcgaaaa ccttcacgtt gccgaggtag
     7801 ggcacggtgt acgtccaccg cgtcgccgcc cagtagttcc agaattccca gagcacaccg
     7861 cacgcagccc cggcggcgag cagcgcgcag aggcgcgagg cgtcgccgcg cgcgaggtcg
     7921 gcgagccacg acggccggcc ggcgcgcgca ttgagcggct cgagcaggag gatgaagccc
     7981 agccagacga ggggcacgag ccaggcgctc accaccagca gtggaagcgc caccgcggcc
     8041 gcgcccactg ccaccgacgc ccgcgccagc gcgggcgtcg gtgcccacgg ccgcacgcgt
     8101 acccgcgcga agaccgtcgc acggagcgcc gcggcggtga ggaacagcgc cgggaggatc
     8161 gtcgcgaacg cccagtcgta gcccacgcgg cgcaggaaga ggtccggctc cagccggtga
     8221 tactgccacc agaggccgcg ggcggcgtcg ccgccgcgcc agaagcgtgg cgcgttgtac
     8281 agctcgaaga gccaccaggc ggcgatcgaa gcgagcgcca ccagcagcag ctccagccgt
     8341 cccgtcgtca ggtacgagcg gccggtgagc cgggcgacga cggcgtcgag cagcagcacg
     8401 tagcctgtcc acacgacggg cgtgaaccac tcgccgacga tccggttccc gccgaacagc
     8461 agcagctccg cggcgacgat gatcccgatc ccggcgaggc cgtgggcggg gacgagacga
     8521 gacgccgggc gagcgggatg tcgagccaag ggcatgctcc cgtgcccgtc cgcggctacc
     8581 gcatcttggc gaggtcgatc tcgaagcggg tgcgctcctg tccctcgggc gtctggatca
     8641 gcaaggtcac gcgggagtcg ccgtccacgt ccttggcggg gaaccagtac acgcagcgcg
     8701 ccaagtagcg cccgccctcg ccccggatcg ccgtccgctc gttctggaca atcgagggct
     8761 ggatgatgcg gtcgccgacg gacagcctcg gcgccaggaa ccgggcgaag tcctccttgt
     8821 cgccgtgcag ctccacccac agcatcaggc ggtcgcg
//
LOCUS       DATIAZ010000360         6200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1036047, whole genome shotgun sequence.
ACCESSION   DATIAZ010000360 DATIAZ010000000
VERSION     DATIAZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036047"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VLF19_12270"
     CDS             <1..275
                     /locus_tag="VLF19_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSE94075.1"
                     /translation="VPVSAAEGKQITTIEGLAAGSVLHKVQKAWLDHEVPQCGYCQAG
                     MIMAVAALLKDKPKPTDADIDAEITNICRCGTFQQVREAIHAAARA"
     gene            288..2468
                     /locus_tag="VLF19_12275"
     CDS             288..2468
                     /locus_tag="VLF19_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSE94076.1"
                     /translation="MITNAPKIARRSFLIGTAAVGGGLALGLNIPLGGGVVRAQDGSP
                     EVNAWVVIRPDDTVVIRIARSEMGQGTLTGLAQMVAEELECDWSKVTTEYPTPGQSVK
                     RKRVWGDFSTGGSRGIRMSHEYVRKGGATARVMLVEAAARAWGVPAAECSAANSVITH
                     KPSGRTTTYGKVAEAAAKLTPPADVALKDPKEWKLIGKPVKRLDTMAKLTGAQVYGAD
                     LKLPGMLNAAIKDCPVFGGKVKSFEAAKVKGMPGVRHVVPVGDSAVAVVADTWWEAKT
                     ALDALPIVWDEGPNAKVSSATIAEMLKAGLDADQAFVHNQNGDAKAAIAGAAKKVEAV
                     YAYPFQNHAPMEPMNATAKYTPERCEVWVPTQNGEAAFAATLAASGLPADKCEVYKIN
                     LGGGFGRRGAFHDYVTQAVQIAKQIPGTPVKLLWSREEDMLHGRYHPVMQAKLTGGLD
                     ANGNLVGLHVRLSGQSILTSVFPQNLQNGKDPLTFQGFWPTGEHSFEYTVTNLLVDHA
                     MRNTHVPPGFWRGVNINQNAIFLECFMDELAHAAGQDALEFRRKLMAKHPKSLAVLNA
                     VADKGGWGKPAPAGVHRGLAHFRCFGSYVAACAEVSVTGGNKVKVQRIVCATDPGHAV
                     NPAQIERQIAGSFVYGLSALFMEECTVKDGHIEQTNFSTYDSMRIAQMPRVESIIMPS
                     GGFWGGVGEPTICVAAPAVLNAFFRATGRRIRTFPLKNHGIQMV"
     gene            2478..2768
                     /locus_tag="VLF19_12280"
     CDS             2478..2768
                     /locus_tag="VLF19_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HSE94077.1"
                     /translation="MPRVLAAAIGFASIAVAVAASAEPPAGAAACSGCHAASASATSP
                     VPRLAGRERAVIVKAMQEFRSGQRSATVMDRIAKGFTDEEVQAIAAWYAAQR"
     gene            2825..4039
                     /locus_tag="VLF19_12285"
     CDS             2825..4039
                     /locus_tag="VLF19_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSE94078.1"
                     /translation="MFPVPTFAQGAAPRVVVVGGGFGGASCARALRTADPRIAVTLVE
                     ANATFTACPFSNAVIGGLRELSEQQFTYERVAAGGIAVVRAAATAVDAAARSVTLADG
                     ARLPYDRLVLAPGIDFRWGALPGYDEAAAERMPHAWRAGPQTLLLRRQLEAMPDGGLV
                     VIAVPANPFRCPPGPYERASLIAHYLKTRKPRCKLIILDAKDAFSKQRLFQNAWKELY
                     PGLLEWVPLSKGGKVTSVDAEARTLVTDFGRHTAAVANVIPPQKAGRIAEVAGVADRS
                     GWCPIDPVTFESKLQAGIHVIGDAAIAGAMPKSAFAANSQAKTCAAAVARLVAGGAPS
                     VPKLINTCYSLVAPDYGISVAGVYQPSSGQLAEVPGSGGVSPAEAPRVTRALEATLAE
                     NWFRTITGEVFG"
     gene            4072..4449
                     /gene="soxX"
                     /locus_tag="VLF19_12290"
     CDS             4072..4449
                     /gene="soxX"
                     /locus_tag="VLF19_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HSE94079.1"
                     /translation="MVGVARGEDAILDSLTGARGDPARGRAIVASRQVGLCLLCHSGP
                     FPEERFQGNLAPDLRGAGSRWSEGQLRLRIVDPGRVNPATIMPAYHRTEGLVRVAPVY
                     RGKPVLSAEQIEDVVAFLTTLRD"
     gene            4581..5054
                     /locus_tag="VLF19_12295"
     CDS             4581..5054
                     /locus_tag="VLF19_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HSE94080.1"
                     /translation="MTRHTSRREFLLATGSAAAGLGLASVVAVEPARATPAGMQEAIR
                     KVVGSARVNTGRVKLELPPLVENGNTVPLVVTVESPMTEADHVRAIHVFTEKNPQPNV
                     AGFRLGPRAGRARVATRIRLADTQTVVAICELSDGSFWSDGVSVVVTLAACLEEG"
     gene            5057..5377
                     /gene="soxZ"
                     /locus_tag="VLF19_12300"
     CDS             5057..5377
                     /gene="soxZ"
                     /locus_tag="VLF19_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HSE94081.1"
                     /translation="MASALINVPPRAKRGEIIEIKTLISHVMETGYRRTQHGAPIPRD
                     IIRLFVCTYNGTEVFRAELHPAIAANPFIAFSTVATESGTITLHWTGDNGFSATESAA
                     ITVD"
     gene            5603..>6200
                     /gene="soxA"
                     /locus_tag="VLF19_12305"
     CDS             5603..>6200
                     /gene="soxA"
                     /locus_tag="VLF19_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HSE94082.1"
                     /translation="MAAPACAGEIPRSERRSGYDFMSRETRAMQDDDTANPGLLWVLE
                     GEALWSRKAGTAGRACADCHGDARASMKGVAARHPSFDAVRRRPISLEQRINLCRTDR
                     QQAPPLAWESRNLLALTAYVARQSRGLPIDPAIDERAQPFLEAGRAAFRRRQGQLNLA
                     CSQCHDDNWGRRLAGNVVPQAHPTGYPLYRLEWQSLGSL"
BASE COUNT          967 a         2135 c         2212 g          886 t
ORIGIN      
        1 tcgtgcccgt cagcgcggcc gaaggcaagc agatcaccac gatcgaaggc ctcgctgcag
       61 gctccgtcct gcacaaggtg cagaaggcct ggctcgacca tgaggtgccg cagtgcggct
      121 actgccaggc gggcatgatc atggcggtgg cggcgctcct gaaggacaag ccgaagccga
      181 ccgatgccga catcgacgcg gagatcacca acatctgtcg ctgtggcact ttccagcagg
      241 tgcgtgaggc catccacgcg gcggccagag cataaggggg cgatgccatg atcaccaacg
      301 ctccgaagat cgctcgccgc tccttcctca tcggcaccgc cgccgtcggc ggcgggctgg
      361 cgctgggtct gaacatcccg ctcggcggtg gcgtcgtgcg cgcccaggac ggctcgcccg
      421 aggtcaacgc ctgggtggtc atccgccccg acgacacggt cgtgatccgg atcgcacgct
      481 ccgagatggg ccagggcacg ctcaccggcc tcgcgcagat ggtggccgag gaactcgagt
      541 gcgactggtc gaaggtgacg accgagtatc cgacgcccgg ccagagcgtc aagcgcaagc
      601 gcgtgtgggg cgacttctcc acgggcggca gccgcggcat ccggatgtcg cacgagtacg
      661 tccggaaggg cggcgccacg gcgcgggtga tgctggtcga agccgcggcc agggcctggg
      721 gagtgccggc cgccgagtgc agcgccgcga acagcgtgat cacgcacaag ccctccggcc
      781 gaacgaccac gtacggcaag gtggcggagg ccgccgcgaa gctcacgccg ccggcggacg
      841 tcgcgctcaa ggatccgaag gagtggaagc tcatcggcaa gccggtgaag cgcctcgaca
      901 cgatggccaa gctcaccggc gcccaggtct acggcgctga tctgaagctg cccggaatgc
      961 tgaatgccgc catcaaggac tgtccggtgt tcggcggcaa ggtgaagagc ttcgaggccg
     1021 ccaaggtcaa gggcatgccg ggcgtccgcc acgtggtgcc ggtcggcgat tcggccgtcg
     1081 cggtggtggc cgacacgtgg tgggaggcca agaccgcgct cgacgcgctg cccatcgtct
     1141 gggacgaggg cccgaacgcc aaggtgtcga gcgccacgat cgccgagatg ctgaaggccg
     1201 gcctcgacgc cgaccaggcg ttcgtccaca accagaacgg cgacgcgaag gccgccatcg
     1261 ccggggcggc gaagaaggtc gaggcggtgt acgcctatcc gttccagaac cacgcgccca
     1321 tggagccgat gaacgccacg gcgaagtaca cgccggagcg ctgcgaggtc tgggtgccga
     1381 cgcagaacgg cgaggccgcg ttcgcggcca cgctggcggc ctcggggctg ccggccgaca
     1441 agtgcgaggt ctacaagatc aatctcggcg ggggcttcgg acggcgcgga gccttccacg
     1501 actacgtgac gcaggctgtc cagatcgcca agcagattcc gggcacgccg gtgaagctcc
     1561 tctggtcgcg cgaggaggac atgctgcacg gccggtatca cccggtcatg caggccaagc
     1621 tcacgggcgg gctggacgcg aacggaaatc tggtcgggtt gcacgtccgt ctgtccggac
     1681 agtcgatcct cacgtccgtg ttcccgcaga atctgcagaa cggcaaggac cccctcacct
     1741 tccaggggtt ctggccgacc ggggagcact cgttcgagta cacggtgacc aacctcctcg
     1801 tcgaccatgc gatgcgcaac acgcacgtcc cgccgggctt ctggcgcggc gtgaacatca
     1861 accagaacgc gatcttcctc gaatgcttca tggacgagct ggcccatgcc gccggccagg
     1921 acgcgctcga gttccggcgc aagctcatgg ccaagcatcc gaaatccctg gccgtgctca
     1981 atgcggtcgc cgacaagggg ggatggggca agccggcgcc ggcaggggtg caccgcggcc
     2041 tcgcccactt caggtgcttc ggcagctacg tggccgcctg cgcggaggtc tccgtcaccg
     2101 gcggcaacaa ggtgaaggtg cagcgcatcg tctgcgcgac cgatccgggg cacgccgtca
     2161 acccggcgca gatcgagcgg cagatcgccg ggtcgttcgt ctacggcctg tccgcgctgt
     2221 tcatggagga gtgcacggtc aaggacggcc acatcgaaca gacgaacttc agcacgtacg
     2281 actcgatgcg gatcgcgcag atgccgaggg tcgagtcgat catcatgccg tccggcgggt
     2341 tctggggcgg ggtcggcgag ccgacgatct gcgtggccgc ccctgcggtc ctgaacgcgt
     2401 tcttcagggc gacgggccgg cgcatccgca cgttcccgct caagaaccac ggcatccaga
     2461 tggtgtaggc gcgggcgatg cccagagtcc tcgccgcggc gatcggattc gcctcgatcg
     2521 ccgtggccgt cgcggcgtcg gccgagccgc cggccggcgc cgccgcctgc tcgggctgtc
     2581 atgcggcctc ggcgagcgcg acgtcgccgg tgccgcggct cgccgggcgc gagcgtgcgg
     2641 tgatcgtcaa ggccatgcag gagttccgct cggggcagcg gtccgccacc gtcatggacc
     2701 ggatcgccaa gggcttcacc gacgaggaag tccaggccat cgccgcctgg tacgccgcgc
     2761 agcggtgacg gcaggccgcc gcgagttcct gaagtcggct gtcgcggtca ccgcctcggc
     2821 cgcgctgttt cccgtgccga ccttcgcgca gggcgccgcc ccacgcgtcg tggtcgtcgg
     2881 cggaggcttc ggcggcgcga gctgcgcccg cgccctccgg acagccgacc cgcgcatcgc
     2941 ggtgacgctg gtcgaggcga acgcgacgtt caccgcctgc ccgttcagca acgccgtgat
     3001 cggcggcctg cgagagctga gcgagcagca gttcacctac gagcgtgtcg ccgccggcgg
     3061 catcgccgtc gtccgggcgg ccgcgaccgc cgtcgacgcc gccgcgcgct cggtcacgct
     3121 ggcggatggt gcgcgattgc cgtacgaccg gctggtgctg gcgccgggta tcgatttccg
     3181 atggggtgcg ctgccgggct acgacgaggc ggcggccgag cgcatgccgc acgcctggcg
     3241 cgcgggcccg cagactctcc tgttgcgccg gcaactcgag gccatgcccg acggcggact
     3301 cgtcgtcatc gcggtcccgg ccaatccgtt ccgctgcccg cccgggccct acgagcgcgc
     3361 cagcctcatc gcgcactacc tcaagacgag gaagccgcgc tgcaagctga tcatcctcga
     3421 cgccaaggac gcgttctcca agcaacggct gttccagaac gcctggaagg agctgtaccc
     3481 gggcctgctc gagtgggtgc cgctgtccaa gggcggcaag gtcacctcgg tggacgccga
     3541 ggcccgcacc cttgtgaccg acttcgggcg gcacacggcg gcggtggcca acgtcatccc
     3601 gccgcagaag gcgggccgca tcgcggaagt cgcaggcgtc gccgaccgca gcggctggtg
     3661 cccgatcgac ccggtgacgt tcgagtcgaa gctgcaagcc ggcattcacg tcatcggcga
     3721 cgccgcgatc gccggcgcga tgccgaaatc ggccttcgcc gccaattcgc aagccaagac
     3781 gtgcgcggcg gcggtcgccc ggctcgtcgc gggcggcgcg ccgtccgtcc ccaagctgat
     3841 caacacctgt tacagcctgg tcgcccccga ctacgggatc tcggtggccg gtgtctacca
     3901 gccgtcgagc gggcagctgg ccgaggttcc cggctcgggg ggcgtcagtc ccgccgaggc
     3961 gccgcgcgtg acgcgtgccc tcgaggcgac cctggccgag aactggttcc gcaccatcac
     4021 cggcgaggtg ttcgggtgag gttggcggcg gcgctcgtcg cggtatgctg catggtgggc
     4081 gtggcacgcg gagaggatgc gatactggat tcgctcacgg gcgccagagg cgatcccgcg
     4141 cgcggccgcg cgatcgtcgc cagccgtcag gtcggcctgt gcctgctctg tcacagcggg
     4201 ccgtttccgg aggagcggtt ccagggcaat ctcgcgcccg atctcagggg agccgggagc
     4261 cgatggtccg aaggccagct gcggctcaga atcgtcgatc ccggccgcgt caacccggcg
     4321 acgatcatgc cggcgtatca tcggacggag gggctggtcc gggtcgcgcc ggtctaccga
     4381 ggtaagcccg tgttgagcgc ggagcagatc gaggacgtgg tggccttcct gacgacgttg
     4441 cgtgactgaa tgaaatcggg gggagcgcgc cgctcccccc gatgaccgca gcgggtctga
     4501 actggaggat tgcgcgggcc aagcccgcgc tcgaaagcgg cgaccatgac acgtgggctc
     4561 ttctcgccga ccggctctca atgacacggc acacttcccg ccgtgagttc ctgctggcca
     4621 ccgggagcgc cgcggccggt ctcgggctgg cgtcggtggt cgcggtcgag cccgcgcgcg
     4681 cgacgcccgc gggaatgcaa gaggccatcc gcaaggtggt gggctcagcg cgggtcaaca
     4741 cgggcagggt gaagctcgag ctcccgccgc tcgtcgagaa cggcaatacc gtgccgctcg
     4801 tggtcacggt cgagagcccg atgaccgagg ccgatcacgt gagggccatt catgtcttca
     4861 cggaaaagaa cccacagccc aacgtcgcgg gcttccgcct ggggccccgc gccgggcggg
     4921 ccagggtggc gacccgcatc cggctcgccg acacgcagac cgtggtcgcg atctgcgagc
     4981 tgagcgacgg ctcgttctgg tccgacggcg tgagcgtcgt ggtgaccctc gcggcgtgtc
     5041 tggaggaggg gtagtcgtgg cgagcgcgct gatcaacgtg ccgccccgcg ccaagcgcgg
     5101 tgagatcatc gagatcaaga cgctgatctc gcacgtgatg gagaccggct atcgccgcac
     5161 ccagcacggc gcgccgatcc cgcgcgacat catcaggctc ttcgtgtgca cctacaacgg
     5221 caccgaggtc ttccgggccg agcttcatcc ggcgatcgcc gcgaatccct tcatcgcgtt
     5281 ctcgacggtc gccacggaga gcggcaccat cacgctccac tggaccggag acaacggctt
     5341 ttcggcgacg gagtcggccg ccatcaccgt cgattgaccg aggggggtgg ggcctcgatg
     5401 gatcgatcgg gggccccgac acggccgggg gcgcacctcg acctcgcgac tcggtcacgg
     5461 gctacgaatg ggcgaccccg acatggcctc tcacacccac cagacactgc gtagcgtcgc
     5521 ggcacggccg tggcgtccct cgacctcgcg attcggtcgg ggcgcgttac ggtgcttctg
     5581 gggtgcggtg tgtgcgatca cgatggcggc gcccgcgtgt gccggggaga ttccccgctc
     5641 ggagcggcgc tcggggtacg acttcatgag ccgggagacg cgcgcgatgc aggacgacga
     5701 caccgccaat cccggcctgc tctgggtgct cgagggtgaa gcgctgtgga gccggaaggc
     5761 cggcaccgcc ggccgcgcct gcgccgattg ccacggcgat gcccgcgcga gcatgaaggg
     5821 tgtggccgcg cgccatccgt cgttcgacgc cgtgcggagg cggccgatca gcctcgagca
     5881 gcgcatcaat ctctgccgga ccgaccggca gcaagcgccc ccgctcgcgt gggagagccg
     5941 gaatctgctg gcgctgacgg cctacgtcgc gcgccagtca cgcggcctgc cgatcgatcc
     6001 cgcgatcgac gagcgggcgc agccgttcct cgaggcaggc cgcgctgcct tccgccggcg
     6061 ccagggtcag ctcaaccttg cctgtagcca atgtcacgac gacaactggg gtcggcggct
     6121 cgcgggcaac gtcgtgccgc aggcgcaccc gaccgggtat ccgctctacc gtctggagtg
     6181 gcagagcctc ggctcgctcc
//
LOCUS       DATIAZ010000361         4895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1300751, whole genome shotgun sequence.
ACCESSION   DATIAZ010000361 DATIAZ010000000
VERSION     DATIAZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300751"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VLF19_12310"
     CDS             <1..266
                     /locus_tag="VLF19_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94083.1"
                     /translation="LDVTGGLAYFGGTGGNRISVSNNVCDGACQTFHGIPVFFQNGAT
                     PANLAIGPNPLKNPDLGALTTSGPGAAAIILDGPTSRVSISGR"
     gene            complement(430..3207)
                     /gene="uvrA"
                     /locus_tag="VLF19_12315"
     CDS             complement(430..3207)
                     /gene="uvrA"
                     /locus_tag="VLF19_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSE94084.1"
                     /translation="MPRPLGLRVEGARQNNLKNVSLEIPHDRLTVITGVSGSGKSSLA
                     FDTIFAEGQWRYIESLSTYARMFLDRIDRPDVDRIEQIRPAVALEQKNPVRTARSTVG
                     TATEVYDYLRLLFAKIGRVHCPTCDLEAVSHSAETIVDALLAEHAGARALVGFTLPVP
                     VGTAAAELWTALTRRGFARVRLGDEVADLAAPPPPALASARDVTVVLDRVVLDAAQRR
                     RLTESVETALREGGGRLEVEVVGAGRRTFAESLRCPACGLALERPQPLLFSFNHPLGA
                     CPECKGFGNVLKYDEALVVPDPTRTLADGAVEPWTHPTGKWYQRQLLKAARRRGLDLD
                     KSWTALTDAERRFVHDGDGAFPGIQGFFEEVESYRYKLHVRVFLSRYRSQSRCPGCAG
                     ARLKPGALTVRVTGRTIADVTALTVEQAARVVAELRLSAWESHVAREILKQLTAKLTF
                     LLRVGLGYLTLARQTRTLSGGEAQRINLANQLGSQLVGTLYVLDEPSIGLHARDTTRL
                     ADLCRELAQAGNTVVVVEHDRGFMEAADHLVELGPGSGDRGGEIVFNGPRAEFLGTSH
                     SLTARYLTGRETIAVPAIRRMGRRHLVLTGAREYNLKDVTLRLPLGTLTVVSGVSGSG
                     KSTLVHDTLYRAVARAFKTDFATPGAYTALTGLEYLKGVRLIDQEPIGRTPRSNPVTY
                     VKAFDEIRKLFAALPRAKALGLGAGAFSFNVPGGRCEKCQGDGFEKLEMYFFEDVYVS
                     CQECEGRRYRPDVLAVKYRGRTIADVLGMTVDEAVDFFTAQAALARRLRVLGDVGLGY
                     LRLGQPATTLSGGEAQRLKIAAELGARAAGDVLYVLDEPTTGLHLDDVKKLLGVLHRL
                     VDAGSTVLVVEHHLDVIKCADWVVDLGPEGGDEGGEIVAEGPPEAVAQTPGSYTGKFL
                     AELLPKNGKSR"
     gene            3262..4620
                     /locus_tag="VLF19_12320"
     CDS             3262..4620
                     /locus_tag="VLF19_12320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSE94085.1"
                     /translation="MADRIRLLVVDDDAEVRSLLERVLGKGGYQPTQASNGAQAMELL
                     GREEFDVALVDIKLPDASGLDILRWARDTDTDTEFIVLTGNADVETAVEAMRLGAYDF
                     IAKPWRNAELIEVIGKAAEKKTLRRENSALREVITRRDGLPQIVGQTPEMQELASIIG
                     RVAASDSPVLIQGESGTGKELVARTIHLKSRRASKPFVSVNCGALPDTLLETELFGHK
                     RGAFSGAVTSRVGLFEAADGGTLFLDEIGEMSAAMQVRLLRVLDSGEVRRVGEERAFH
                     VDARIVAATNKDLSREAGEGRFRWDLFYRVSTITVPVPPLRRRRADIAPLVQHFTAPA
                     ARGGKALRFAPEAMERLMQYDWPGNIRELRNMVERLMILHEGDEVQANELPVELSTQS
                     RPSTEPADAALVPLAEMERRYVERVLASTNWNKAQAARVLDIDIKTLNKKIRDFNLTR
                     LA"
BASE COUNT          756 a         1687 c         1760 g          692 t
ORIGIN      
        1 ttctcgacgt gaccggcggc ctcgcttact tcggtgggac gggcggtaac cggatcagcg
       61 tgtcgaacaa cgtctgcgac ggcgcctgcc agacgttcca tgggattccg gtcttcttcc
      121 agaacggcgc cacgccagcc aacctcgcca tcgggccgaa ccccctcaag aatcccgatc
      181 tcggcgcgct gaccacgtcc ggccccggag ccgcggcgat cattctggac gggccgacga
      241 gccgggtcag catcagcggg cggtagccgg ccgtggcggc cggcaggcga gcagcatcgc
      301 caccgcgacc acgttgaccg ccgccgagat cgcgaaggcc ggggcatagc tgctcgtggt
      361 gtcgtggatg gcgccgccgc cgccgaccca gccgtagaag atcgccgccg gtctctcgcg
      421 gcggcgcgtc taacggctct tgccgttctt gggcaacagc tcggccagga acttgccggt
      481 gtacgagccg ggcgtctgcg cgacggcctc cggcgggccc tcggcgacga tctcgccgcc
      541 ctcgtcgcca ccttccggac cgaggtcgac gacccagtcc gcgcacttga tgacgtcgag
      601 gtggtgctcg accacgagca cggtgctgcc cgcgtccacg aggcggtgca gcacgccgag
      661 cagcttcttg acgtcgtcga ggtgcagccc cgtggtgggc tcgtcgagga cgtagaggac
      721 gtcgcccgcg gcgcgggcgc cgagctcggc ggcgatcttc agccgctgcg cctcgccccc
      781 cgacagcgtc gtggccggct gcccgagcct caggtagccg agaccgacgt cgccgagcac
      841 gcgcaggcgg cgcgcgagcg cggcctgggc ggtgaagaag tcgacggcct cgtcgacggt
      901 catgccgaga acgtcggcga tggtccgccc gcggtacttg acggccagca cgtccggacg
      961 gtagcgccgg ccctcgcact cctggcacga gacgtacacg tcctcgaaga agtacatctc
     1021 gagcttctcg aagccgtcgc cctggcactt ctcgcagcgc ccgcccggca cgttgaaaga
     1081 gaaggccccg gcgccgagcc cgagcgcctt cgcgcggggc agcgccgcga acagcttgcg
     1141 gatctcgtcg aaggccttca cgtaggtgac ggggttcgag cggggcgtgc ggccgatggg
     1201 ctcctggtcg atcagccgga cgcccttgag gtactcgagc cccgtcagcg ccgtgtaagc
     1261 gcccggcgtc gcgaagtcgg tcttgaacgc gcgggccacg gcccgataga gcgtgtcgtg
     1321 gacgagcgtc gacttcccgg agcccgacac gccgctcacc acggtcagcg tcccgagggg
     1381 caggcggagg gtgacgtcct tgaggttgta ctcgcgggcg cccgtgagga cgagatggcg
     1441 tcggcccatc cgtcggatgg cggggacggc gatcgtctcg cggccggtga ggtagcgcgc
     1501 ggtgagcgag tgcgaggttc cgaggaactc ggcccgcggc ccgttgaaga cgatctcgcc
     1561 gccgcgatcg ccggagccgg ggcccagctc gacgagatgg tcggcggcct ccatgaagcc
     1621 gcggtcgtgc tcgaccacga ccacggtgtt gcctgcctgc gccagctccc ggcagaggtc
     1681 ggccaggcgc gtggtgtcgc gcgcgtgcag gccgatcgag ggctcgtcga gcacgtacag
     1741 cgtgccgacg agctgggagc cgagctggtt ggcgaggttg atgcgctggg cctcaccgcc
     1801 ggagagcgtg cgcgtctgcc gggcgagcgt gagatagccg agacccaccc gtagcaggaa
     1861 cgtgagcttg gcggtgagct gcttgaggat ctcccgcgcc acgtgactct cccacgccga
     1921 gaggcggagc tcggccacga cgcgtgccgc ctgctcgacg gtcagcgccg tgacgtccgc
     1981 gatcgtgcgg cccgtcacgc gcacggtgag cgcgcccggc ttgaggcggg cgccggcaca
     2041 gccgggacag cgcgactggc tccggtagcg cgagaggaac acgcgcacgt ggagcttgta
     2101 gcggtacgac tccacctcct cgaagaaccc ctggatgccc gggaacgcgc cgtcgccgtc
     2161 gtggacgaag cgacgctcgg cgtcggtgag cgccgtccac gacttgtcga ggtcgagccc
     2221 gcggcggcgg gcggccttca ggagctggcg ctggtaccac ttgcccgtgg ggtgcgtcca
     2281 cggctcgaca gcgccgtcgg ccagcgtgcg tgtcggatcc ggcacgacga gcgcctcatc
     2341 gtacttcagc acgttgccga agcccttgca ctccgggcac gccccgagcg ggtggttgaa
     2401 ggagaaaagc aacggctgcg gacgctcgag cgcgagcccg cacgccgggc accgcaggct
     2461 ctccgcgaac gtgcggcgcc cggcgccgac gacctcgacc tcgaggcggc cgccgccctc
     2521 gcgcaacgcc gtttcgaccg actccgtgag gcggcggcgc tgcgcggcgt ccagcacgac
     2581 gcggtcgagg accaccgtga cgtcgcgcgc cgacgcgagg gcagggggcg gcggcgcggc
     2641 caggtcggcg acctcgtcgc ccaggcggac gcgcgcgaag ccccgccgcg tcagcgccgt
     2701 ccacagctcg gccgccgcgg tcccgaccgg gacgggcagc gtgaagccga cgagcgcgcg
     2761 ggcgcctgcg tgctccgcga gcagggcgtc cacgatcgtc tccgccgagt gggagaccgc
     2821 ttcgagatcg cacgtcgggc agtggacacg gccgatcttg gcgaagagca ggcggaggta
     2881 gtcgtagacc tcggtcgccg tgcccacggt ggagcgggcg gtgcggaccg ggttcttctg
     2941 ttcgagcgcg acggcgggac ggatctgctc gatgcggtcc acgtccgggc ggtcgatgcg
     3001 gtcgaggaac atccgcgcgt aggtcgagag cgactcgatg taccgccact ggccctcggc
     3061 gaagatcgtg tcgaaggcca gggacgactt accggagccg gagacgccgg tgatgacggt
     3121 gagccggtcg tgcgggatct cgagcgagac gttcttgagg ttgttctggc gggctccctc
     3181 gacgcgaagc cccaggggcc gaggcatccg aacagtcctc ctgaggtgga tatcttagcg
     3241 gaagtgctat catggcgcca gatggcggat cgtatccggc tcctcgtcgt ggacgacgac
     3301 gccgaggtgc gctcgctcct cgagcgggtg ctgggcaagg gcggctacca gccaacccag
     3361 gccagcaacg gcgcgcaggc catggagctg ctcggccgcg aggaattcga cgtggcgctg
     3421 gtcgacatca agctgccgga cgcctccggc ctcgacatcc tgcgctgggc gcgcgacacg
     3481 gacaccgaca ccgagttcat cgtcctcacc gggaacgccg acgtcgagac ggccgtcgag
     3541 gcgatgcgac tcggcgccta cgacttcatc gccaaaccct ggcgcaacgc cgagctgatc
     3601 gaggtgatcg gcaaggccgc cgagaagaag acgctccggc gcgagaactc ggcgctgcgc
     3661 gaggtcatca cgcggcgcga cggcctgccg cagatcgtcg gccagacgcc ggagatgcag
     3721 gagctggcgt cgatcatcgg gcgagtggcg gccagcgaca gccccgtcct catccagggc
     3781 gagagcggca cgggcaagga actggtggcg cgcacgatcc acctcaagag ccggcgcgcg
     3841 tccaagccct tcgtgtcggt caactgcggg gccctgcccg acaccctgct cgagacggag
     3901 ctcttcggcc acaagcgcgg cgccttcagc ggcgcggtga cctcgcgcgt gggcctcttc
     3961 gaggcggccg acggcggcac cctgttcctc gacgagatcg gcgagatgtc ggcggcgatg
     4021 caggtccgcc tgctgcgcgt gctcgactcc ggagaggtgc gccgggtggg tgaagagcgc
     4081 gcgttccacg tggacgcgcg gatcgtcgcc gcgacgaaca aggacctgag ccgtgaggcc
     4141 ggcgagggcc gcttccgctg ggatctcttc taccgcgtca gcacgatcac cgtgccggtg
     4201 ccgccgctcc ggcgccggcg ggcggacatc gcgccgctgg tgcagcactt cacggcgccg
     4261 gcggcccggg gcggcaaggc cctgcggttc gcgccagagg ccatggagcg gctgatgcag
     4321 tacgactggc caggcaacat tcgcgagctt cgtaacatgg tcgagcgcct gatgatcctc
     4381 cacgagggcg acgaggtgca ggccaacgag ctgcccgtgg agctgtccac gcagagccgg
     4441 ccctccaccg agccggccga cgccgcgctg gtaccgctcg ccgagatgga gcgtcgctac
     4501 gtcgagcgcg tgctcgcgtc caccaactgg aacaaggccc aggccgcgcg tgttctcgac
     4561 atcgacatca agaccctcaa caagaagatc cgggacttca acctcaccag gctggcctga
     4621 agccgggccg cctgccccga gagccgtttg cccctgaaag cgacctagta gaaactccct
     4681 tctcccagga ggtcctccct gccggcacga ttgtgaaact ctaacaaacg atttggaatt
     4741 aggtccttac ggttctgatg gtgtggcacc tcggttgcct acaaggctcg tatcatggca
     4801 ctattcgccg cccgcccgat gagcctggac acgcccgtcg acagcctcga tccgcggccc
     4861 tccggcatca cgttgtggcg gccggagatc gttct
//
LOCUS       DATIAZ010000362         4793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_247821, whole genome shotgun sequence.
ACCESSION   DATIAZ010000362 DATIAZ010000000
VERSION     DATIAZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247821"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VLF19_12325"
     CDS             <1..1140
                     /locus_tag="VLF19_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HSE94086.1"
                     /translation="TFALTEGAIAAGCRVFAGYPITPATDIAEYMSKRLPQVGGYYMQ
                     AEDELAGMHMCAGASLGGLKAMTATSGPGYTLMHDAYGWAITNEIPLVVVDAMRVGPI
                     SGITGAPGQGEFYIARYASHGGNFEAIVLSPSSVQEAFWLTIDAFNLAERFRTPVTIL
                     TDQVVSDMWEDLFVPDDYESLDFVVPRKSNLMMPFFPLGSADIDVPPNVIGHGTGVCV
                     SAYTHTEEGYDIEEMEAQWAQTYRLVNKIRHHRAELTRYETVGLDDAEVVAVAYGANA
                     RTVKTGVLEARARGVRAGFVRPITLWPFLDELFAADRHYVVCELNYDGQLVREVARAA
                     PDSKKVHFMGKSAELHTVGEVVAGLEGVAGDGRVPEIPYIWTEVR"
     gene            1145..1993
                     /locus_tag="VLF19_12330"
     CDS             1145..1993
                     /locus_tag="VLF19_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSE94087.1"
                     /translation="MNPLAKKYLRSSQIPTKACHGCGIGMIENWLLQAIDELGLKQED
                     VIFGTGIGCVGRQTFATWGGDNFGATHGRALAVATGVKLARPDRKILVVVGDGDAASI
                     GTSHLVHAARRNLGVTVICEDNMGYESTGGQFSPTTPAGYVTNSSPYGMVEPSFDPCD
                     VVKAAGATFVAETTATQWHQTVKTVKKALQNDGFSFVHVRFPCNENFGAYALGTRDTL
                     KNLEWIYEHTQGRKADGSETDFVWETGIKHDASNSRPEFTRLLRDMNDRVRADMASKT
                     TTQKPG"
     gene            1993..2541
                     /locus_tag="VLF19_12335"
     CDS             1993..2541
                     /locus_tag="VLF19_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSE94088.1"
                     /translation="MAASARARTEILISGFGGQGVIRMGQIIGLCAIKQGHRVTMLKS
                     HGTETRGGYVRAQLVISPDYVDSPVVENADVFVAFSAPAYKKFFDLCRGKVLYDPEMV
                     TEVRPDAPERHVAVPATQLSKERFGKELFANMIMLGALTRLSGMDHEAMKHAMVEIIP
                     RFHEQNLAALDAGHALPVLTPS"
     gene            2732..4225
                     /locus_tag="VLF19_12340"
     CDS             2732..4225
                     /locus_tag="VLF19_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSE94089.1"
                     /translation="MPGWWLLERNVARFGSRVALRELDHDTLAERRALTYEALWRAAR
                     GTAAGLRARGVGPGVRVGYCLPNGADLVTGYYSTWAAGGTVVPANPLAHEPELTEQLG
                     DAAVGLIVGAAASAAEQAAARLGVEFVDVEAFRTLAGQEPAEPAPCPPDDVAVILYTG
                     GTTGTPKGAMLSHRNIVVNTVQFAEWYAFAPGEETAVAAIPMYHSGGMSGVMNVPLYA
                     AATLLVFGRFGASGAARTVTRYRATRLFGVPTMFIALLNDAEGRRADYSSLRACRTNA
                     APLPPAVKRAFDGLVGREVLVEGYGLTETSPLTHANPIGRAKAGSIGVPLPDTDARIV
                     DESGADVTPGEPGELLIRGPQVMLGYWRRPAETAAALAGGWLRTGDIARMDGEGYFSI
                     VDRSKDVINTAGFKVWPREVEETLYLHPAVRQAAVVGVPDDYRGEAVKAFVVLRDGPA
                     ARPSAVDIVDFCRARLTPYKVPRLVEFRDELPMTTTGKLLRRELRGN"
     gene            4428..>4793
                     /locus_tag="VLF19_12345"
     CDS             4428..>4793
                     /locus_tag="VLF19_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HSE94090.1"
                     /translation="MTHARRVPVLLALAVLLLVGSYPAGAAAQEKFPSRPIDFIVPWG
                     TGGGADSMARQVATLAQPILGVALPASNVPGAAGNTGLAQVLNGKPDGYTIAVYIQDT
                     LMTIPMGLARHKVEDLEWIV"
BASE COUNT          739 a         1724 c         1669 g          661 t
ORIGIN      
        1 accttcgccc tcacggaggg cgccatcgcc gccggctgcc gcgtgttcgc gggctatccg
       61 atcacgccgg cgacggacat cgccgagtac atgtccaagc gccttcccca ggtcggcggc
      121 tactacatgc aggccgagga cgagctggcg ggcatgcaca tgtgcgcggg cgcgagcctg
      181 ggcgggctca aggcgatgac ggccacctcg ggccccggct acacgctcat gcacgacgcc
      241 tacggctggg cgatcaccaa cgagatccct ttggtggtcg tggacgccat gcgggtcggg
      301 cccatcagcg gcatcacggg cgcgccggga cagggcgagt tctacatcgc gcgctacgca
      361 tcgcacggcg gaaacttcga ggccatcgtg ctctcgccct cgtcggtgca ggaggcgttc
      421 tggctgacca tcgacgcctt caacctggcc gagcgcttcc gcacgccggt caccatcctc
      481 accgaccagg tcgtctccga catgtgggag gacctcttcg tccccgacga ctacgagagc
      541 ctggacttcg tcgtgccacg caagtcgaac ctcatgatgc cgttcttccc cctgggcagc
      601 gcggacatcg acgtgccgcc caacgtgatt ggccacggca ccggcgtctg cgtgtcggcc
      661 tacacgcaca cggaggaagg ctatgacatc gaggagatgg aggcgcagtg ggcgcagacc
      721 taccggctgg tgaacaagat ccgccaccac cgggccgagc tgacgcgcta cgagacggtg
      781 gggctcgacg acgccgaggt ggtggccgtg gcctacggcg ccaacgcccg cacggtgaag
      841 acgggcgtgc tggaggcgcg ggcgcgcggc gtccgggccg gcttcgtgcg ccccatcacg
      901 ctctggccgt tcctcgacga gctgttcgcc gccgaccggc attacgtggt ctgcgagctg
      961 aactacgacg gccagctcgt gcgcgaggtg gcgcgcgcgg cgccggacag caagaaggtc
     1021 cacttcatgg gtaagagcgc cgagctccac accgtcggcg aggtcgtcgc cgggctcgaa
     1081 ggcgtcgccg gggatgggcg cgtccccgag atcccttaca tatggaccga ggtgcgatga
     1141 acccatgaac cccctggcga agaaatacct gagatcgagc cagatcccca ccaaggcctg
     1201 ccacggctgc ggcatcggca tgatcgagaa ctggctcctg caggccatcg acgagctggg
     1261 gctcaagcag gaggacgtca tcttcggcac cggcatcggc tgcgtgggcc gccagacctt
     1321 cgccacgtgg gggggcgaca acttcggcgc cacccacggc cgcgccctgg cggtggccac
     1381 cggcgtcaag ctcgcgcgac cggaccggaa gatcctggtc gtcgtcggcg acggtgacgc
     1441 cgcgtccatc ggcacctcgc acctggtcca cgccgcccgc cgcaacctcg gcgtgacggt
     1501 gatctgcgag gacaacatgg gctacgagtc caccggcggc cagttctcgc cgacgacgcc
     1561 ggcgggctac gtcacgaact cgagccccta cggcatggtc gaaccgtcct tcgacccctg
     1621 cgacgtagtg aaggccgccg gggcgacctt cgtagccgag accacggcca cccagtggca
     1681 ccagacggtc aagaccgtga agaaggcgct ccagaacgac ggcttctcgt tcgtccacgt
     1741 gcgcttcccc tgcaacgaga acttcggcgc gtacgcgctc ggcacccgcg acacgctcaa
     1801 gaacctcgag tggatctacg agcacaccca gggccgcaag gccgacggca gcgagacgga
     1861 cttcgtctgg gaaacgggga tcaagcacga cgccagcaac agtcggccgg agttcacgcg
     1921 cctgctgcgc gacatgaacg atcgcgtgcg ggccgacatg gcgagcaaga cgacgacgca
     1981 gaagccgggc tgatggccgc gtccgccaga gcccgcaccg agatcctcat ctccggcttc
     2041 ggcggccagg gcgtgatccg catggggcag atcatcggcc tctgcgcgat caagcagggt
     2101 caccgggtga ccatgctcaa gagccacggc accgagacgc gcggcggcta cgtgcgggcg
     2161 cagctcgtga tctcgccgga ctacgtggac agccccgtgg tggagaacgc cgacgtcttc
     2221 gtggccttct cggcccccgc gtacaagaag ttcttcgacc tctgccgcgg caaggtgctg
     2281 tacgaccccg agatggtgac cgaggtccgg cccgacgcgc ccgagcgcca cgtggccgtg
     2341 cccgccaccc agctctccaa ggagcgcttc ggcaaggagc tcttcgccaa catgatcatg
     2401 ctgggtgcgc tgacgcgcct ctccggcatg gaccacgagg cgatgaagca cgcgatggtg
     2461 gagatcatcc cgcgcttcca cgagcagaac ctcgccgcgc tcgacgccgg ccatgcgctc
     2521 cccgtcctca ccccctcctg acgtgcgtga ctcgccgccg gacaacgacg agaggccggc
     2581 tcgagcgtcg gctccggcgg acccggttca tggggagcgc ctcggaggcg gccgtccaag
     2641 ccccctccgc gaggaaacga tccacgccga ccatcgttcg tggcaccgcg tctggcccgc
     2701 gcacgtgggg cacacgctgg attacccgcg cgtgccgggc tggtggctgc tcgagaggaa
     2761 cgtggcccgc ttcgggtcgc gcgtggccct gcgcgagctc gatcacgaca cgctcgccga
     2821 gcgccgggcg ctcacctacg aggcgctctg gcgcgccgcc cgcggcacgg ccgccgggct
     2881 gcgcgcgcgc ggcgtcggcc ccggcgtccg cgtcggctat tgcctgccca acggcgccga
     2941 cctcgtgacc gggtactact cgacgtgggc ggccggcggc accgtggtcc cggccaaccc
     3001 gctcgcgcac gagcccgagc tgaccgagca gctcggcgac gcggcggtcg ggctgatcgt
     3061 gggcgcggcg gcgagcgcgg ccgagcaggc ggccgcccgg ctcggcgtcg agttcgtgga
     3121 cgtcgaggcc ttccgcaccc tggccggcca ggagccggcc gagcccgcgc cgtgcccccc
     3181 ggacgacgtc gccgtcatcc tctacaccgg cggtaccacc ggcacgccga agggcgcgat
     3241 gctgagccac cgcaacatcg tcgtgaacac ggtgcagttc gccgagtggt acgcgttcgc
     3301 gccgggggaa gagaccgcgg tcgcggcgat ccccatgtac cactcgggcg ggatgtcggg
     3361 cgtgatgaac gtcccgctct acgccgccgc gacgctgctc gtgttcggcc gtttcggcgc
     3421 cagcggcgcg gcgcggaccg tcacgcgata ccgggcgaca cgcctcttcg gcgtgccgac
     3481 catgttcatc gcgttgctca acgacgccga gggccggcgc gccgactact cgtcgctccg
     3541 cgcctgccgc accaacgcgg cgcccctgcc gcccgccgtg aagcgcgcgt tcgacgggct
     3601 cgtcgggcgt gaggtgctgg tggaaggcta cgggctcacc gagacgagcc cgctgactca
     3661 cgccaacccg atcggccggg ccaaggccgg ctcgatcggc gtgcccctgc ccgacaccga
     3721 cgcgcgcatc gtggacgagt cgggcgccga cgtgacgccg ggcgagcctg gcgagctgct
     3781 catccgcggg ccgcaggtga tgctcgggta ctggcggcgg ccggcggaga cggcggcggc
     3841 gctggccggc ggctggctgc gcacgggcga catcgcgcgc atggacggcg aggggtactt
     3901 ctcgatcgtc gaccgcagca aggacgtgat caacacggcg ggcttcaagg tctggccgcg
     3961 cgaggtggag gagacgctct acctgcaccc cgccgtgcga caggcggcgg tcgtcggcgt
     4021 ccccgacgac taccggggcg aggcggtgaa ggcgttcgtc gtgctccgcg acggcccggc
     4081 ggcacggccg agcgccgtgg acatcgtgga cttctgccgt gcacggctga cgccctacaa
     4141 ggtcccgcgg ctggtggagt tccgtgacga gctgccgatg acgacgaccg gcaagctgct
     4201 gcggcgcgag ttgcggggta attgacatgg gggccccgag atggccccca agccccccgg
     4261 gcggctcgtc acgacccggc ttagccgtgt cgcgcctcgg tctctcgcgc aacgatcgcc
     4321 tgacatggga gcccggcatg gccttcgagc ccccgacgct cggcgcgccg atgctgcgcc
     4381 gtgacgcgcc tcgatatccg gcaggctgct ggcctgagag gagagccatg acccacgcta
     4441 gacgcgttcc cgtgctgctc gcgctcgccg tgctgctgct cgtaggatcg taccccgccg
     4501 gcgcggcggc gcaggagaag ttcccgagcc ggcccatcga cttcatcgtg ccgtggggga
     4561 ccggcggcgg cgccgactcg atggcgcgcc aggtggccac gctggcccag ccgatcctcg
     4621 gtgtcgcgct gccggcctcg aacgtgccgg gtgcggccgg caacaccggg ctcgcccagg
     4681 tgctcaacgg caagcccgac ggctacacga tcgccgtgta catccaggac acgctcatga
     4741 ccatcccgat gggcctcgcc cgccacaagg tcgaggacct cgagtggatc gtg
//
LOCUS       DATIAZ010000363         4884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_775257, whole genome shotgun sequence.
ACCESSION   DATIAZ010000363 DATIAZ010000000
VERSION     DATIAZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775257"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VLF19_12350"
     CDS             complement(<1..705)
                     /locus_tag="VLF19_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HSE94091.1"
                     /translation="MTFRQNDEEIGLLEPLDAEGTEQGPAPEETRAEDPVRLYFQEMG
                     KVRLLTARQEVEIGRRIEDAQIALRRALAGVPLAVRRLLDLGEAVRGGRTPGDEMLLP
                     PAGGEVDGRELKRLYRALGRIRRLEAEVVRLTESLTDRRLGAASRANYTKWIEANRES
                     IQTVIAELPLDPALIDRLVAEVRAVAERGAEGARAAGLTPAVLRSRLAEISEHDAAVR
                     QAKRELTEANLRLVVSI"
     gene            897..1268
                     /locus_tag="VLF19_12355"
     CDS             897..1268
                     /locus_tag="VLF19_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE94092.1"
                     /translation="MAGRALVIDDDAAVRRLLVEFLRVLGWETEEAADGVKGLALFDR
                     AKPALVVTDVRMPGLSGWDVVVAIRRRSPRVPIVIVSGADVTPPDPALARAPALMILT
                     KPVGLTQLGDAVARLTAAPSA"
     gene            1341..2042
                     /locus_tag="VLF19_12360"
     CDS             1341..2042
                     /locus_tag="VLF19_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSE94093.1"
                     /translation="MPARLATEVLRASPVFAGLPAREIEALAAVAREETYRPRTYVFE
                     EGDPATWVCIVKSGHVKIVRHSRAGRDVVLELLGPGEVFGGVAVMERRPYPAAAQATE
                     TSVVLKIPAAAALALAERHPSVIREMALVIGRRLRAAHDSVKSLSVDPVEARLAGAVV
                     RLAGREGTRSKEGVTLPFHLTRQSLADMTGTTVETTIRVVSRWIRDRIVAEDGGHLVL
                     RDLDALRALAEGESA"
     gene            2042..2473
                     /locus_tag="VLF19_12365"
     CDS             2042..2473
                     /locus_tag="VLF19_12365"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSE94094.1"
                     /translation="MSLAARSVHVLAAITWVGGMVFIALVLVPVTRRLTDPALRTRLV
                     HEIGVRFRTVGWIALGLLVLSGFVNLWMRPYLLGVPRFHVKLGLVVVALALSVLHDVV
                     LGPRAGAPGADPALRVRASWVARVNMLVVLAIVVLGLALRG"
     gene            2537..2920
                     /locus_tag="VLF19_12370"
     CDS             2537..2920
                     /locus_tag="VLF19_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94095.1"
                     /translation="MLGGYLVVGEFLLGTYPSRLLITAHVHLLLVGFVLMIVMGVATW
                     MFPRPARDDARYRPGLAETVYWVMTCATALRAAAEILAAATGAPPLRALVAVGGLGQL
                     AGALMFAVNMWPRVRMPAVPPPPPR"
     gene            3025..3264
                     /locus_tag="VLF19_12375"
     CDS             3025..3264
                     /locus_tag="VLF19_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HSE94096.1"
                     /translation="MADTCSCCGGVEIGKPAAAPARVDANRTVNELIAASPAALAVMQ
                     RSGIDHCCGAALTLTEAAASAGADLPALLAALAEA"
     gene            3261..4049
                     /locus_tag="VLF19_12380"
     CDS             3261..4049
                     /locus_tag="VLF19_12380"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HSE94097.1"
                     /translation="MISADTGMAELLDAHPQLVEVLAGFHPHFALLRSRLLRKVVAPR
                     VTVAQAARIAGVPVEALLTTVQRAVGEPEVAPAPRETPEREAVAEPRPAALDDRRAVY
                     VDVREDIARGAEPFARIVAAAKALGDRETLVLRAPFEPIPLYDVLGRRGLAHWTERHG
                     AGDWSVWFYRGPGASGPGATAAGGAAGRERRVVLDVRGLEPPQPMVRVLERLDAMSAG
                     DELLVVHDRKPLFLYPQLDDRGFTHDTREMAPGRVEILIRRRSA"
     gene            4049..>4884
                     /locus_tag="VLF19_12385"
     CDS             4049..>4884
                     /locus_tag="VLF19_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94098.1"
                     /translation="MISPLTARAPSAGLPLAYLVAATAAFAAAAASVPWLATELAGHY
                     YQPRILALTHLVTLGWITVTIMGASYQLIPIVLARPVWSERLARWQLGVLVAGVAGMV
                     GHFWIGQWSGLVWSAGLVGLGILAHLVNAALSVRGLARWTFTARLVVLALAGLALTAL
                     FGLALGADKLWRFLPGPLFANLHAHVHLALLGWVLPMVIGVAARVYPMFLLANEPDGW
                     GGRIQLWSLGLGVPTVVAGLLVQRPALAVAGALFMVATVAAHLAWVAGMVAHRKRPAL
                     DWG"
BASE COUNT          546 a         1797 c         1796 g          745 t
ORIGIN      
        1 gatcgagacc accagccgga ggttcgcctc ggtcagctcg cgcttggcct ggcgcaccgc
       61 cgcgtcgtgc tcggagatct cggccagacg cgagcgcagc acggccggcg tgagaccggc
      121 cgcccgcgcg ccctcggcgc cacgctcggc gacggcgcgc acctcggcca cgaggcggtc
      181 gatcagcgcc gggtccagcg gcagctcggc gatcaccgtc tggatcgact cgcggttggc
      241 ctcgatccac ttcgtgtagt tcgcgcgcga ggcggcgccg agccgccgat cggtcagtga
      301 ctcggtgagc cgcacgacct cggcttcgag gcggcggatc cgcccgagcg cccggtaaag
      361 acgcttgagc tcgcggccgt cgacctcgcc ccccgccggg ggcaggagca tctcgtcgcc
      421 gggtgtccgg cccccgcgca ccgcctcgcc gaggtcgagc agccggcgga ctgcgagagg
      481 gacgcccgcg agggctcgcc ggagcgcgat ctgggcgtcc tcgatccgcc gcccgatctc
      541 cacctcctgg cgggccgtca gcagccgcac cttgcccatc tcctggaagt agaggcggac
      601 gggatcctcg gcccgggtct cttccggcgc aggtccctgc tcggtcccct cggcgtcgag
      661 gggctccagg agcccgatct cctcgtcgtt ctggcgaaac gtcatgtatc cagcttcctc
      721 cgggccgtca gacgggcccg cccgtacacc tattcatgtg ttcagatgcc ggacggggtg
      781 atggcgatgc gcggcctgga ccaagggcca aggcgcggcc ctcgggacgt cgtggtagcc
      841 tccgccggta aatcgagcac gaaggcggca tggcgccgcc tcgaaccggg ggggcgatgg
      901 caggccgcgc gctggtgatc gacgacgatg ccgcggttcg ccgcctcctc gtggagttcc
      961 tccgggtcct cggatgggag accgaggagg cggccgacgg ggtcaagggc ctggccctgt
     1021 tcgaccgtgc caagcccgcc ctcgtcgtca ccgacgtccg catgcccggc ctgagtggct
     1081 gggacgtcgt ggtcgcgatc cgccgccggt cgccgcgggt accgatcgtg atcgtgtcgg
     1141 gcgctgacgt cacaccgccg gacccggccc tggccagggc cccggcgctc atgatcctca
     1201 ccaagcccgt cggcttgacc cagctcggcg acgcggtcgc gcgcctgacc gccgcgccgt
     1261 ccgcctgagc gccgcccggc gccgggggga ccggtgcctc gcccggcggc ctggcgcggg
     1321 cgtgctacca ttccgcccgg atgccagcgc gtctggccac cgaggtgctc agagccagtc
     1381 cggtcttcgc cgggctcccc gccagagaga tcgaggcgct cgccgccgtc gcgcgcgagg
     1441 agacgtatcg cccgcgcacg tacgtgttcg aggaaggcga cccggccacc tgggtctgca
     1501 tcgtgaagtc cgggcacgtg aagatcgtcc ggcactcgcg tgccggcagg gacgtcgtgc
     1561 tcgagctgct gggtcccggc gaggtgttcg gaggcgtcgc ggtgatggag cgtcgcccct
     1621 atccggccgc cgcccaggcg accgagacga gcgtcgtgct gaagatcccg gccgcggccg
     1681 cccttgcgct ggccgagcgg cacccctcgg tgatccgcga gatggctctg gtgatcggac
     1741 gccggctgcg cgccgctcac gactcggtga agtcgctgtc cgtcgatccg gtggaggccc
     1801 ggctggccgg ggcggtggtg cgcctggccg ggcgcgaggg cacgcggagc aaggagggcg
     1861 tgacgctgcc cttccacctg acgcgccaga gcctcgccga catgacgggc acgacggtgg
     1921 agacgacgat ccgcgtcgtg agccgctgga tccgcgaccg gatcgtcgcg gaggacggcg
     1981 gccacctggt gctgagggac ctcgacgcgt tgcgggcgct ggccgagggg gaatcggcgt
     2041 agtgtccctc gccgcacgca gcgtgcacgt gctcgccgcc atcacgtggg tcggcggcat
     2101 ggtcttcatc gcgctcgtgc tggtaccggt cacgcggcgc ctcaccgacc ccgcgctgcg
     2161 cacccggctc gtccacgaga tcggcgtgcg attccgcacg gtcggctgga tcgcgctcgg
     2221 gctgctggtg ctcagcggat tcgtgaacct ctggatgcgt ccgtacctcc tcggcgtgcc
     2281 gcgcttccac gtcaagctcg ggctcgtggt cgtggcgctg gcgctcagcg tgctgcacga
     2341 cgtcgtgctc ggtccgcgcg ccggcgcgcc cggggccgat cccgcgctcc gcgtgcgcgc
     2401 ctcctgggtg gcgcgggtga acatgctggt cgtgctggcg atcgtcgtcc tgggtctcgc
     2461 gctgcggggg tgaccgctgc ccgcgctggt ccgccgctac atcaagacga gcttcgtctt
     2521 cctcggcctc gggctgttgc tcggaggcta cctggtcgtc ggcgagttcc tcctcgggac
     2581 gtatccctcg cggctgctca tcaccgcgca cgtgcacctg ctcctcgtcg gcttcgtgct
     2641 catgatcgtc atgggcgtgg cgacgtggat gttcccacga cccgcgcgtg acgacgcccg
     2701 ctaccggccc gggctcgccg agaccgtgta ctgggtgatg acgtgcgcga ccgcgctgcg
     2761 ggcggcggcg gagatactcg ccgcggccac cggcgcgccg ccgctgcgcg cgctcgtcgc
     2821 cgtcggcggc ctcggtcagc tcgccggcgc gctcatgttc gcggtcaaca tgtggccccg
     2881 tgtccggatg cccgccgttc ccccaccgcc gccgcgctga gcccaccaga gcgccgtgtg
     2941 tgctccggtt caccacctat gaccggcgtc ataggaggcg gcgggactcg cgcgtaggct
     3001 gggggccgga gggagggccc cccgatggcc gacacgtgct cctgctgtgg tggtgtggag
     3061 atcggcaagc ctgcggcggc gccggctcgc gtggacgcga atcgaacggt gaacgaactc
     3121 attgcagcct cacccgcggc gctcgccgtc atgcagcgct ccggcatcga tcattgctgc
     3181 ggcgcggcac tcacgctcac cgaggcggcg gcgtccgccg gcgccgacct tcccgcgctg
     3241 ctggcagccc tggccgaggc atgatctcgg ccgacaccgg gatggcggag ctcttggacg
     3301 cccaccccca gctcgtcgag gtgctggccg ggttccaccc gcacttcgcc ttgctccgga
     3361 gccgcctgct gcgcaaggtc gtcgcgccac gcgtgacggt ggcgcaggcg gcgcgcatcg
     3421 cgggcgtacc cgtcgaggcc ctcctgacca cggtgcagcg cgccgtcggc gagcccgagg
     3481 tggcgccggc gccgcgcgaa acgcccgaac gggaggcggt cgccgagccg cggccggcgg
     3541 cgctcgacga ccggcgcgcc gtgtacgtcg acgtccgcga agacatcgcg cgcggcgccg
     3601 agcccttcgc gcgcatcgtg gcggcggcga aggcgctggg cgaccgtgag acgctcgtcc
     3661 tgcgcgcgcc gttcgagccg atcccgctgt acgacgtgct cggccggcgg gggctcgcgc
     3721 actggaccga gcggcacggt gccggcgact ggtcggtctg gttctatcgc ggccccggcg
     3781 cgtccggccc cggcgcgacg gcggccggcg gcgcggcagg gcgggagcgc cgcgtggtcc
     3841 tcgacgtgcg cggtctcgag cccccgcagc cgatggtgcg cgtgctcgag aggctcgacg
     3901 cgatgagcgc cggtgacgag ctgctggtgg tgcacgaccg caagccgctg ttcctctacc
     3961 cgcagctcga cgaccgcggc ttcacgcacg acacgcgtga gatggcgccc gggcgcgtcg
     4021 agatcctgat ccgccgccgg agcgcctgat gatctctccg ctgacggccc gcgccccatc
     4081 ggccgggctg ccgctggcgt atctggtcgc cgcgacggct gcattcgcgg ccgcggcggc
     4141 aagcgtgccg tggctcgcga cggagctcgc cggccactat taccagccgc gcatcctcgc
     4201 tctcacccac ctcgtgacgc tcggctggat caccgtgacg atcatgggcg cgagctatca
     4261 gctgatcccc atcgtgctcg cgcgccccgt gtggagcgag cggctggccc gctggcagct
     4321 cggcgtgctc gtcgccggcg tggccggcat ggtcggtcac ttctggatcg ggcaatggtc
     4381 cggtctcgtg tggagcgccg ggctcgtcgg gctgggcatc ctcgcgcacc tcgtcaacgc
     4441 cgcgctgagc gtgcgcgggc tcgcgcgctg gaccttcacc gcccgcctgg tggtgctggc
     4501 gctcgccggg ctggccctca cggcgctctt tggcctcgcg ctcggcgccg acaagctctg
     4561 gcgcttcctg ccgggccccc tgttcgcgaa cctccacgcg cacgtgcacc tggcactgct
     4621 cggctgggtg ctgccgatgg tgatcggcgt ggccgcgcgc gtctacccga tgttccttct
     4681 ggcgaacgag ccggacggct ggggtgggcg gatccagctc tggagtctcg gcctcggcgt
     4741 gcccacggtc gtcgcggggc tcctcgtcca gcgcccggcg ctcgccgtcg ccggagcgct
     4801 gttcatggtc gcgacggtgg ccgcgcacct ggcctgggtc gcggggatgg tggcgcatcg
     4861 caagcggccc gcgctcgact gggg
//
LOCUS       DATIAZ010000364         5113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1171770, whole genome shotgun sequence.
ACCESSION   DATIAZ010000364 DATIAZ010000000
VERSION     DATIAZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171770"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VLF19_12390"
     CDS             complement(<1..265)
                     /locus_tag="VLF19_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSE94099.1"
                     /translation="MYERGVRAPPSSPVVLFCVRDAASSGVDALRGGGAEAMHFELSD
                     ELKALVQVAREFAAERIVPFVEEWDKTHYFPYKEVIKPMAELGF"
     gene            659..880
                     /locus_tag="VLF19_12395"
     CDS             659..880
                     /locus_tag="VLF19_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HSE94100.1"
                     /translation="MADHVVEAPAPGKILKVYVEQGKPVVDGDRLCLMEALKMELPIV
                     APAGGTVKALHVSAGQNVEAGDLLAVIEE"
     gene            895..1038
                     /locus_tag="VLF19_12400"
     CDS             895..1038
                     /locus_tag="VLF19_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94101.1"
                     /translation="MLNATWFVLAGMGIVFATLGLLALVMTALDRWCGPETGGHRKRA
                     GRA"
     gene            1085..2167
                     /locus_tag="VLF19_12405"
     CDS             1085..2167
                     /locus_tag="VLF19_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002550036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HSE94102.1"
                     /translation="MLALGGLLIYLGIARQVEPVLLVPIGMGIILANIPHGGLTEAGG
                     LLAVLRTIGIDTELFPLLIFIGVGAMIDFGSLLERPYTVFLGAAAQFGIFGTFFLAYF
                     LGDGGLRLFDFSFKDAVSIGIIGSADGPTSIYVATVLGSKYAPAIVVAAYSYMALVPI
                     IQPPIMRVLTTAAERRLAMPARARKVSRRTRVLFPVVTILVAGLIAPKSTPLIGTLMF
                     GNLLRESGVLDRLSQAAQNELANIVTILLGLAVGGMMAAEEFLRPETIMIYVMGVVAF
                     AVGTGAGVLLGKGMYVASGKRINPLIGAAGISAFPMAARVVQKVGLEANPHNHLIMHA
                     AGANTAGQIASVVAGGAVLMLVPFFA"
     gene            2241..3257
                     /gene="phnD"
                     /locus_tag="VLF19_12410"
     CDS             2241..3257
                     /gene="phnD"
                     /locus_tag="VLF19_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HSE94103.1"
                     /translation="MAPPATRLAPLIVLVVIAIVAGAALAADPCAHRGKLDVLYCDEN
                     RDGIADPPKDGAKLVSPDPLVFTYAPVEDPAVYEGAWGDFLKHVEKVTGKKTQYFGLQ
                     NYAAQIEAMRSGRLHLSAYSTGSLVYAVHLAGAVPFAIMRDAKGPSGYHLVLITQARN
                     DKIKSVADLKGKRVAHVSQTSSSGHQAPVYFFSKMGVVPGKDYQIVFSGKHDNSALGV
                     VNGDYDAAAVADTVLERMVARNVIKAADFKVIYTSPVFPTAGFAHSHNLEPGLVEKIK
                     EAFYSFKFEGTSVGREFKGRTGFLPVNYAKDWESVVGILEANGVAFTRESPEYRKLQK
                     PGGE"
     gene            3273..4037
                     /gene="phnC"
                     /locus_tag="VLF19_12415"
     CDS             3273..4037
                     /gene="phnC"
                     /locus_tag="VLF19_12415"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HSE94104.1"
                     /translation="MLEVTALRKTYATGDAALRGVAFAIDRPEPVFVIGSSGAGKSTL
                     LRCINRLVEPDGGSVKLDGVEITTLAGRALREARRAMGMIFQEFNLVERLTVMENVLS
                     GRLGYVSAWRAWRRRFPPDAVEAAFRTLARVGLEGFENKRADALSGGQRQRVGIARAL
                     VQRPKILLVDEPTSSLDPRTSEAVMRLIVELAAEDGIPALVNIHDVPLARAFARRVIG
                     LRDGVIVYDGPEAGLSAGALERIYGGSWAAAGEAAR"
     gene            4097..4837
                     /gene="phnE"
                     /locus_tag="VLF19_12420"
     CDS             4097..4837
                     /gene="phnE"
                     /locus_tag="VLF19_12420"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HSE94105.1"
                     /translation="MAPLAWTAAAAFLAWNAVSLEVDPARIARGLSRFGDAAGRAFPP
                     DFHRLELLAGGVLESIEIATLSTLLGVVFGIPVAVLASRNVVPLPVYGAGRGTITLGR
                     TFHELIVGILFVKAVGFGPLAGVLTLTVNSVGFFSKLLAEHIETIDRGQIEAVRATGA
                     SGAQVLIFGVLPQVLPRVIGLTIYQWDIHLRQSTIIGIVGAGGIGATLLNAFSRYDYD
                     FALAILLVIVAIVLVGEAISALARKAVQ"
     gene            4929..>5113
                     /locus_tag="VLF19_12425"
     CDS             4929..>5113
                     /locus_tag="VLF19_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HSE94106.1"
                     /translation="MSAREWRRFSPARAAARMLGQLALATAVAVMLARLGVRWEYVAD
                     APAQIGDLLGRMFPPEWS"
BASE COUNT          690 a         1789 c         1804 g          830 t
ORIGIN      
        1 ggaatcccag ctcggccatg ggcttgatca cttccttgta ggggaagtag tgcgtcttgt
       61 cccactcctc gacgaacggg acgatcctct ccgcggcgaa ttcccgggcc acctgcacga
      121 gggccttcag ctcgtcggac agctcgaagt gcatggcttc ggcgccccct ccccggagcg
      181 catcaacgcc ggaactcgcc gcgtcccgaa cacagaacaa taccacgggc gaagatggtg
      241 gagcccgcac gccccgctcg tacatcagcc cttccgggta ccatgtgccc ggccgtcacg
      301 cccgtggtcg gccggttcgg cgcgacggcc gcgcgctcgg gcaccaggga gacggagcgt
      361 gtgaacggac gagggagctg gtggtggggg gggcggccgt gccgcggctc ctcggggggc
      421 tcgtgactgg ttcctggggt tcctgccggt cgggccgacg ctgttcgcgc tccagccggt
      481 gctgatctac cgggtgtgtg cgccgaccgt gcggcgtggc tgagggcgga ccgcactccg
      541 cccgccggcg acgccgcgag gggcttatga tgaggtgcgc ggcgcgtttg actcactggg
      601 gtgcgcgtgg tagcgtgccg cggatttccg accgacatca gaaagagggt gacgacaggt
      661 ggccgatcac gtcgtggaag cgccggcgcc gggcaagatc ttgaaggtgt acgtcgagca
      721 gggcaagccc gtggtggacg gagaccgcct ctgcctcatg gaggcgctga agatggagct
      781 gccgatcgtt gctcccgcgg gcggcacggt gaaggcgctc cacgtttccg ccgggcagaa
      841 cgtcgaggcc ggcgatctgc tggccgtcat cgaggagtag ccgcaggggt tcgcgtgctc
      901 aacgccacct ggttcgtcct ggctggcatg ggcatcgtct tcgccacgct gggcctgctc
      961 gctctcgtga tgaccgctct cgaccgctgg tgcgggcccg agaccggcgg ccaccggaag
     1021 agggccgggc gtgcctgagg gcgcgggttt cggccttctc gccctcacgt ggcagggcgc
     1081 cgtcatgctc gcgctcggcg ggctcctcat ctaccttggc atcgcccggc aggtcgagcc
     1141 ggtgctcctc gttcccatcg ggatggggat catcctggcg aacatccccc acggcgggct
     1201 caccgaagcg ggcgggctcc tcgccgtgct gcgcaccatc ggcatcgaca ccgagctgtt
     1261 cccgctcctg atcttcatcg gcgtgggcgc catgatcgac ttcggctcgc tgctggagcg
     1321 gccgtacacc gtgttcctcg gcgccgccgc ccagttcggc atcttcggca cgttcttcct
     1381 ggcctacttc ctcggcgacg gcggcctccg cctcttcgac ttcagcttca aggacgcggt
     1441 ctcgatcggc atcatcggct ccgccgacgg ccccacgtcc atctacgtcg ccaccgtcct
     1501 cggctccaag tacgcgcccg ccatcgtggt ggcggcgtat tcctacatgg ccctggtgcc
     1561 catcatccag ccgcccatca tgcgcgtgct cacgacggcg gccgagcggc gcctggcgat
     1621 gcccgcgcgg gcccggaagg tgtcccggcg cacccgcgtg ctgttcccgg tcgtcacgat
     1681 cctcgtcgcg ggtctgatcg cgcccaagtc cacgcccttg atcggcaccc tcatgttcgg
     1741 caacctcctg agagagtcgg gagtgctgga tcgcctctcg caggcggcgc agaacgagct
     1801 ggccaacatc gtcacgatcc tcctcggact cgccgtggga gggatgatgg cggcggagga
     1861 gtttctgaga ccggagacca tcatgatcta cgtcatgggg gtggtggcct tcgccgtggg
     1921 caccggcgcc ggggtgctcc tgggcaaggg gatgtacgtc gcctccggca agcggatcaa
     1981 tcccctgatc ggtgcggccg ggatctccgc cttccccatg gcggcgcgcg tcgtccagaa
     2041 ggtggggctc gaggccaacc cgcacaacca tctgatcatg cacgccgccg gcgccaacac
     2101 cgccggccag atcgcctcgg tggtggccgg aggcgccgtg ctgatgctcg tgccgttctt
     2161 cgcctgatgc cgttgcgttg cgctcccggc gaggtccgcg gcgcggtgag gtgctagagt
     2221 acgcgccggg aggactatcg atggcaccgc ccgccacccg cctcgcaccg ctgatcgtgc
     2281 tcgtcgtgat cgccatcgtc gcgggcgccg cgcttgcggc cgacccgtgc gcgcaccgcg
     2341 gcaagctcga cgtgctctac tgcgacgaga accgcgacgg catcgccgac ccgcccaagg
     2401 acggcgcgaa gctcgtgagc cccgatccgc tcgtcttcac gtacgcgccg gtcgaggatc
     2461 cggccgtgta cgagggcgcc tggggcgact tcctcaagca cgtcgagaag gtgaccggca
     2521 agaagacgca gtacttcggg ctgcagaact acgcggcgca gatcgaggcc atgcgctccg
     2581 gccggctgca cctctcggcg tactcgacgg gaagcctcgt ctacgccgtg cacctggccg
     2641 gcgcggtgcc gttcgcgatc atgcgcgacg ccaaggggcc gtcgggctac cacctcgtgc
     2701 tgatcaccca ggccaggaac gacaagatca agtccgtggc cgacctcaag ggcaagcggg
     2761 tcgcgcacgt ctcccagacg tcgagctccg gccaccaggc gccggtctac ttcttctcga
     2821 agatgggcgt ggtgccgggc aaggactacc agatcgtctt ctccggcaag cacgacaaca
     2881 gcgcgctcgg cgtcgtgaac ggagactacg acgccgccgc ggtggcggac acggtgctgg
     2941 agcgcatggt cgcgcgcaac gtgatcaagg ccgccgactt caaggtgatc tacacctcgc
     3001 cggtgttccc caccgcgggg ttcgcccaca gccacaacct cgagcccggg ctcgtcgaga
     3061 agatcaagga ggcgttctac tccttcaagt tcgaagggac gagcgtcggc cgcgagttca
     3121 aggggcgtac gggcttcctg ccggtgaact acgcgaagga ctgggagtcg gtggtcggca
     3181 ttctcgaggc caacggcgtg gcctttaccc gggagtcgcc ggagtacagg aagctgcaga
     3241 agcccggcgg ggagtaggcc gcgcccgtcc cgttgctcga ggtcacggcg ctgcgcaaga
     3301 cgtacgcgac gggcgacgcc gcgctgcgcg gcgtcgcctt cgccatcgac cgacccgagc
     3361 ccgtcttcgt gatcggctcc tcgggcgcgg gcaagtccac cctgctccga tgcatcaacc
     3421 ggctcgtgga gcccgacggg ggctcggtga agctcgacgg ggtggagatc accaccctcg
     3481 ccggccgcgc gttgcgcgag gcccgccgcg cgatggggat gatcttccag gagttcaacc
     3541 tcgtcgagcg tctcacggtc atggagaacg tgctctccgg acgcctcggc tatgtgtccg
     3601 cgtggcgggc gtggcgccgc cgcttccccc cggacgccgt ggaggcggcg ttccggacgc
     3661 tcgcgcgcgt gggcctggag ggattcgaga acaagcgcgc cgacgcgctg tcgggcggcc
     3721 agcgtcagcg cgtgggcatc gcgcgggccc tggtgcagcg gcccaagatc ctccttgtgg
     3781 acgagcccac ctcgagcctc gacccgcgca cctccgaggc cgtcatgcgg ctcatcgtgg
     3841 agctggccgc cgaggacggc atccccgcgc tggtgaacat tcacgacgtg ccgctggccc
     3901 gcgcctttgc ccgtcgcgtg atcgggttgc gcgacggggt catcgtgtac gacggaccgg
     3961 aggccggcct gtcggccggg gcgctcgagc gcatctacgg cggcagctgg gcggccgccg
     4021 gcgaggccgc acggtgagcg ccgggggccc gccgccgcgc gtgtggcggc ctccgagcgt
     4081 gtggccgcgc ccgtggatgg cgcccctcgc ctggacggcg gccgcggcct tcctcgcctg
     4141 gaacgcggtc agcctcgagg tcgaccccgc gcgcatcgcc cgggggctct cgcgattcgg
     4201 cgacgccgcc gggcgcgcgt ttccccccga ttttcaccgc ctggagctgc tcgccggcgg
     4261 cgtgctcgag tcgatcgaga tcgcgacgct ctcgacgctg ctcggcgtcg tgttcggcat
     4321 ccccgtggcg gtgctggcgt cgcgcaacgt cgtgcccctg ccggtctacg gggccggccg
     4381 gggaacgatc acgctgggcc ggaccttcca cgagctgatc gtgggcatcc tcttcgtgaa
     4441 ggccgtgggg ttcgggccgc tggccggcgt gctgacgctg acggtgaact cggtgggctt
     4501 cttctcgaag ctgctcgccg agcacatcga gacgatcgac cgcgggcaga tcgaggcggt
     4561 gcgggccacc ggcgcgtcgg gcgcgcaggt gctgatcttc ggtgtcctgc cccaggtact
     4621 gccgcgcgtg atcgggctca cgatctatca gtgggacatc cacctgcggc agtccacgat
     4681 catcggcatc gtgggcgccg gcggcatcgg cgccacgctg ctgaacgcgt tctcccgcta
     4741 cgactacgac tttgcgctgg cgatcctgct cgtcatcgtc gcgatcgtcc tcgtcggcga
     4801 agccatcagc gccctcgccc gcaaggccgt ccagtgacgg gttctgtcga cgaccgtccg
     4861 gcgccggcgt ccggcggggg cgtcccgacg acccggtccc gaacgaccgg aggtcggctc
     4921 ggcgtcggat gagcgcgcga gaatggcggc ggttctcccc ggcgcgcgcc gccgcgcgca
     4981 tgctgggaca gctcgcactg gccaccgccg tggcggtcat gctggcgcgg ctgggcgtcc
     5041 gctgggagta cgtcgccgac gcgcccgcgc agatcggcga cctgctcggg cgcatgttcc
     5101 cgccggagtg gtc
//
LOCUS       DATIAZ010000365         3189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_647794, whole genome shotgun sequence.
ACCESSION   DATIAZ010000365 DATIAZ010000000
VERSION     DATIAZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647794"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VLF19_12430"
     CDS             complement(<1..1284)
                     /locus_tag="VLF19_12430"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSE94107.1"
                     /translation="MSSPSLVIRNGRVLDPGNAVDAVADVLVEGGQVARVGPRLEPPA
                     GAEVIDATGKVVCPGFIDLHVHLREPGFEYKETVATGTRAAAAGGFTAVCCMANTNPV
                     NDSGSITEYILDRARTEGSGVRVYPIGAVTRGLRGEELAELAELAESGCVAFSDDGKC
                     VMNAALYRRAMEYTLPFGTPVVSHAEDDRLAKGGAMHEGVVSTELGLPAAPAAAEDVM
                     VARDIILAELTGAHVHIAHLSTAGAVRLVRDAKARGVRVTAEVTPHHLVLTDEAVRTF
                     DPNTKMSPPLRSKRDVEALMEALADGTIDCVATDHAPHASSEKEGEYDQAAFGVVGLE
                     TALAVLLDRLVRPGIVPLETLVARLSRDPARLLNLPGGRLSPGAPADVTILDLDRELT
                     VDPARFHSRSRNTPFAGWTLRGGPWMTIVGGRKVFS"
     gene            complement(1281..2216)
                     /locus_tag="VLF19_12435"
     CDS             complement(1281..2216)
                     /locus_tag="VLF19_12435"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HSE94108.1"
                     /translation="MAWTRKDLLAMRDLDRGEITLLLDTAASLQEIATREIKKVPALR
                     GKTVVNLFYEPSTRTRTSFEIAGKWLSADVINFTASASSASKGESMLDTAKNIAAMSP
                     DVVVVRHSSAGAAALVARELTCSIVNAGDGAHEHPTQALLDLLTIREKKGHLEGLHVA
                     IVGDIAHSRVARSNIQGMRKLGMTVTVAGPPTLIPPFVQELGVKVAHRLEDAIRDVDV
                     IMMLRLQHERMTAGLIPSLREYSRYWGLTLDRLRAHARPDVLIMHPGPVNRGIELSPE
                     VADGAFSVILDQVAKGVAVRMGVLFLLAGARAEHA"
     gene            complement(2222..2788)
                     /gene="pyrR"
                     /locus_tag="VLF19_12440"
     CDS             complement(2222..2788)
                     /gene="pyrR"
                     /locus_tag="VLF19_12440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSE94109.1"
                     /translation="MSGALPPRFREKAQVLDEHALDRALTRIAHEILERNAGAKDLAF
                     VGLRTRGVTIAQRLAQKIARIDGTTLPVGTLDVSLYRDDLGLRSAPVVRGTEVPFALK
                     DKTVVLVDDVLFTGRTIRAALDAIIDLGRPRMVQLAILIDRGHRELPIRPDYVGKNLP
                     TSRRESVAVRLREHDGEDRVVIEEPEEA"
     gene            complement(2905..>3189)
                     /locus_tag="VLF19_12445"
     CDS             complement(2905..>3189)
                     /locus_tag="VLF19_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE94110.1"
                     /translation="DLSHMAFLAAVAAHMGAPDDLRALVDTITIHPARIMRLPDRGLA
                     PGGRADLVLWECERVEDVIAALAPRSLVMKDGRVTVVHERSVREPWREPA"
BASE COUNT          446 a         1123 c         1150 g          470 t
ORIGIN      
        1 ggagaacacc ttcctgccgc ccacgatcgt catccacggc ccgccgcgga gcgtccagcc
       61 ggcgaacggc gtgttccggc tacgggagtg aaaccgcgcg ggatccaccg tgagttcccg
      121 gtcgaggtcg aggatcgtga cgtcggcggg cgcccccggc gacaggcggc cgccgggcag
      181 gttgagcagg cgcgcggggt cgcggctgag ccgcgcgacc agcgtctcga ggggcacgat
      241 cccggggcga acgaggcggt cgagcagcac ggccagcgcg gtctccagcc cgacgacccc
      301 gaaggcggcc tgatcgtact cgccctcctt ctcgctcgag gcgtgcggcg cgtggtccgt
      361 cgcgacgcag tcgattgtgc cgtcggcgag ggcctccatc agggcttcca cgtcgcgctt
      421 gctccggagc ggcgggctca tcttggtgtt ggggtcgaac gtccggaccg cctcgtcggt
      481 cagcacgagg tgatgtggcg tgacctccgc ggtcaccctg acgccgcggg ccttggcgtc
      541 gcgcacgagc cgcacggcgc ccgccgtcga caggtgggcg atgtgcacgt gggcacccgt
      601 cagctcggcc aggatgatgt cgcgggcgac catcacgtcc tcggcggcgg ccggcgccgc
      661 cgggagcccc agctccgtgg agacgacgcc ctcgtgcatt gcgccgccct tcgccagccg
      721 atcgtcctcg gcatgactga cgaccggcgt gccgaagggc agcgtgtact ccatggcccg
      781 ccggtagagc gcggcgttca tcacgcactt gccgtcgtcg gagaacgcga cgcagccgga
      841 ctcggccagc tcggcgagct cggccagctc ctccccccgg agcccgcggg tcaccgcgcc
      901 gatcggatag acccgcacgc cgctgccctc ggtgcgcgcc cggtcgagga tgtactccgt
      961 gatcgagccg ctgtcgttca cggggttcgt gttggccatg cagcagacgg cggtgaagcc
     1021 gccggccgcg gccgcgcgcg tgcccgtggc cacggtctcc ttgtactcga agcccggctc
     1081 gcgcaggtgg acgtgcagat cgatgaagcc cgggcagacg accttgcccg tcgcgtcgat
     1141 cacctcggcg ccggccggcg gctcgagccg cgggccgacc cgggcgacct ggccgccctc
     1201 gacgagcacg tcggcgacgg catcgacggc attgcccgga tcgaggaccc gaccgttccg
     1261 gatcacgagg ctcggcgagc tcatgcgtgc tccgcgcggg cgcccgcgag caggaacagc
     1321 acgcccatcc tcaccgcgac ccccttggcg acctggtcca ggatcaccga gaacgccccg
     1381 tcggcgacct ccggcgacag ctcgatgccg cggttgaccg ggccgggatg catgatcagc
     1441 acgtcgggcc gcgcgtgggc ccggagccgg tcgagcgtga gcccccagta gcgcgaatat
     1501 tcccgcagcg agggaatgag gccggccgtc atgcgctcgt gctgcagccg cagcatcatg
     1561 atcacgtcca cgtcgcggat ggcgtcctcg agccggtggg cgaccttcac gcccagctcc
     1621 tgcacgaacg gcgggatcag ggtgggcggc ccggccacgg tgacggtcat gccgagcttg
     1681 cgcatgccct ggatgttcga gcgggccacg cggctgtgcg cgatgtcacc gacgatggcc
     1741 acgtgcagcc cctcgaggtg gcccttcttc tcgcggatcg tgaggaggtc gagcagggcc
     1801 tgggtcggat gctcgtgggc gccgtccccg gcgttgacga tggagcaggt gagctctcgg
     1861 gccaccagcg cggcggcgcc ggcggaggag tgccggacga cgaccacgtc cgggctcatc
     1921 gccgcgatgt tcttcgcggt gtcgagcatg gactcgccct tgctggcgct cgaggccgac
     1981 gccgtgaagt tgatgacgtc ggcggacagc cacttgccgg cgatctcgaa ggacgtccgg
     2041 gtgcgagtgg acggctcgta gaagaggttc accaccgtct tgccacgcag cgccggcacc
     2101 ttcttgatct cccgcgtcgc gatctcctgg agcgacgccg ccgtgtcgag gagcagcgtg
     2161 atctcgcccc ggtcgagatc gcgcatggcc aagaggtcct tccgtgtcca cgccatcgtg
     2221 ctcacgcctc ctcgggttcc tcgatcacca ctcggtcctc accgtcgtgc tcgcgcagcc
     2281 gcaccgcgac gctctcgcgc cgggaggtgg gcaggttctt gccaacgtag tcgggacgga
     2341 tcggcagctc ccggtgcccg cgatcgatca ggatcgcgag ctgcaccatg cgcggccggc
     2401 ccaggtcgat gatggcgtcg agggcggccc ggatggtccg gccggtgaag agcacgtcgt
     2461 ccacgagcac gaccgtcttg tccttgagag cgaacggcac ctcggtgccg cggaccaccg
     2521 gcgcgctgcg caggccgagg tcgtcacgat agagcgagac gtcgagggtg ccgacgggta
     2581 gcgtcgtgcc gtcgatgcgg gcgatcttct gggccaagcg ctgggcgatg gtgacgccgc
     2641 gggtgcggag gcccacgaac gccaggtcct tggcgccggc gttgcgctcg aggatctcgt
     2701 gggcgatgcg ggtcagcgcc cgatcgaggg cgtgctcgtc gaggacctgg gccttctctc
     2761 ggaaccgcgg aggcagggca ccactcatca tcgctccttg cggacctcgc cgggcccgct
     2821 taaaggagtt cacgtctcgc aatcgcaccc aactctacac agaatgtggc cccgttgtca
     2881 accggagccc ctaggggtgg ggcgtcaggc cggctcgcgc cacggctcgc gcacgctccg
     2941 ctcgtgcacc acggtcacgc ggccgtcctt catcacgaga ctccgcggag cgagtgccgc
     3001 gatcacgtcc tccacgcgct cgcactccca gagcacgagg tcggcacggc cgcccggggc
     3061 cagcccacgg tcgggcagtc gcatgatccg cgccgggtgg atcgtgatgg tatcgacgag
     3121 ggcgcggagg tcgtcgggcg cccccatgtg cgcggcgacg gcggcgagga acgccatgtg
     3181 ggagaggtc
//
LOCUS       DATIAZ010000366         3638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_250918, whole genome shotgun sequence.
ACCESSION   DATIAZ010000366 DATIAZ010000000
VERSION     DATIAZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250918"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VLF19_12450"
     CDS             <1..1053
                     /locus_tag="VLF19_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSE94111.1"
                     /translation="EVVAWVGVATALFAATIATVQTDIKRVLAYSTVSQLGYMFAAVG
                     LGAHAAGIFHLVTHAFFKALLFLGAGSVIHGLAGEQDLRKMGGLAPKMVLTTVTMAIG
                     AAGLAGVPGLAGFFSKDEILAAAFATGHRAQWVLLLVGAFLTAFYSFRLVFLAFLGAP
                     RMPREVAHHVHESPPAMTVPLVVLAILTAVAGWAVGVPSEAGTRFARFLGSVFPLHHG
                     GGHGGFVGYVLLVLNVVVAAAGIVLAWLTHGARSVRPETIGRRRNAVHALLLNAWYVD
                     RLYDVAVVRPLYALSVFLARVFDLGVIDGLVNATGRAVAAWAAAGRRLQTGYVVNYAL
                     TMLVGAVVVVGYLLAR"
     gene            1050..2591
                     /locus_tag="VLF19_12455"
     CDS             1050..2591
                     /locus_tag="VLF19_12455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSE94112.1"
                     /translation="MTVGLLTVVTFLPAAAALALLLVPRRADTLVRGGALAAAVATFV
                     VSLPLYFAFDAASAEYQFVEYAAWIPSLGVGYHVGVDGISLLLVLLTTLLTPIALASA
                     AHAVEDRAKEFGVVMLLLETGMTGVFVSLDLFLFYVFWEAMLIPMYFVIGVWGGANRI
                     YAAVKFVLYTMAGSVLMLVAILALYFQHGAATGTHTFDLPTLARWVMEPGPAQTLMFL
                     AFAFAFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFCLPLFPHAS
                     LAFGPLVFTLAVVGIVYGAWVSTVQPDLKKLVAYSSVSHLGFVMLGIFTLNSQGLVGG
                     VIQMINHGLSTGALFLMVGMIYERRHTRLIADFGGLWAVTPAFSALFLIVVLSSLGLP
                     GLNGFVGEFLILVGAFRVNGLLAAAATTGIIFAAVYLLWMYQRVVFGPLTHEENRRLP
                     DLTPREWALVAPLVLLIVWIGVYPAPFTRPTEATVEALIAQVQTKADVTVAGAGPTRP
                     QLARP"
     gene            2588..>3638
                     /locus_tag="VLF19_12460"
     CDS             2588..>3638
                     /locus_tag="VLF19_12460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSE94113.1"
                     /translation="MTGVPVPQVALGALLPALIVVATALVVLMLDLVPSRGGKTHLAV
                     VALAGVVGALLATVSAWGGAGRAFRDMVVLDNYALFFHVVVCYAAALVLLVSVDYLRR
                     AGAESGEYYALVLFSASGMLLLTSANDLIVVFLAVELMSLSLYVLSGLFKRRQEAGEA
                     SMKYFLLGVFASAFLLYGIALVYGATGTTNFDRIAAAAAGPRDPLLLVGLALLLVGFG
                     FKISSAPFHMWAPDVYEGAPTSVTAFIATGSKAAVFAALIRLAVTGVRTFQADAAALL
                     WGLAAVTMTVGNVVAIAQSNLKRMLAYSSIAHVGYMLIGLVVGGTAGAAAVLFYLLAY
                     TFTTAGAFGVIALCER"
BASE COUNT          387 a         1347 c         1219 g          685 t
ORIGIN      
        1 gaggtcgtcg cctgggtggg cgtggccacc gcgctgttcg cggccacgat cgccaccgtg
       61 cagaccgaca tcaagcgcgt gctggcctat tcgacggtga gccagctcgg ctacatgttc
      121 gccgcggtgg ggctcggcgc ccacgccgcc gggatcttcc acctggtgac acacgccttc
      181 ttcaaggcgc tcctcttcct cggcgccggc tcggtgatcc acgggctcgc cggggagcag
      241 gacctccgga agatgggcgg gctcgcgccc aagatggtgc tcacgaccgt caccatggcc
      301 atcggcgccg ccggcctcgc cggcgtgccc gggctcgccg gcttcttctc caaggacgag
      361 atcctcgccg cggccttcgc caccggccat cgcgcacagt gggtgctgct cctcgtcggc
      421 gcgttcctca ccgcgttcta ctcgttccgc ctggtcttcc tcgccttcct gggcgcaccg
      481 cgcatgccgc gcgaagtcgc ccaccacgtg cacgagtcgc cgccggcgat gacggtgcct
      541 ctcgtcgtgc tggccatcct cacggcggtg gcgggctggg ccgtgggcgt gccctccgag
      601 gcgggcaccc gcttcgcgcg cttcctgggg tcggtgttcc cgctccacca cggaggcggt
      661 cacggcggct tcgtcggcta cgtcctgctc gtcctcaacg tcgtcgtcgc ggccgcgggc
      721 atcgtgctcg cctggttgac ccacggcgcc cgctccgtgc gtccggagac gatcggccgc
      781 cggcgtaacg ccgtccatgc cctgctgctc aacgcctggt acgtcgaccg gctgtacgac
      841 gtcgccgtcg tccgccccct gtacgcgcta tcggtgttcc tcgcgcgcgt gttcgacctc
      901 ggcgtcatcg acggcctggt gaacgcgact ggccgcgcgg tcgccgcgtg ggccgccgcc
      961 ggccgccgcc tgcagacggg atacgtcgtg aactacgcgc tgaccatgct cgtgggcgcg
     1021 gtcgtcgtgg tcgggtacct gctcgcccga tgaccgtcgg cctgttgacg gtggtgacgt
     1081 tcctgccggc ggccgccgcg ctggcgctgt tgctggtgcc tcgccgcgcc gacaccctgg
     1141 tccgtggcgg cgcgctggcc gcggccgtgg ccacgttcgt ggtgtcgctg ccgctgtact
     1201 tcgcgttcga cgcagcctcg gccgagtacc agttcgtcga gtacgcggcc tggataccct
     1261 cgctcggcgt cggctatcac gtcggcgtcg acggcatcag cctgctgctg gtgctcctca
     1321 ccaccctcct cacgcccatc gcgctggcgt cggcggcgca cgccgtcgag gaccgcgcca
     1381 aggagttcgg cgtcgtcatg ctgctcctcg agacgggcat gaccggcgtg ttcgtgagcc
     1441 tcgacctctt cctcttctac gtgttctggg aggccatgct gatcccgatg tacttcgtca
     1501 tcggggtctg gggcggcgcc aaccggatct acgctgcggt caagttcgtc ctctacacca
     1561 tggccggctc cgtgctgatg ctggtggcga tcctcgccct ctacttccag cacggcgccg
     1621 cgaccggcac gcacaccttc gacctgccca cgctggcgcg atgggtcatg gagccggggc
     1681 ccgcgcagac cctgatgttc ctggcgttcg cgttcgcgtt cgccatcaag gtgccgctgt
     1741 tcccgttcca cacgtggctg cccgacgcgc acgtggaggc ccccacggcg ggttccgtga
     1801 tcctggccgg cgtgctgctc aagatgggga cctacgggtt cctccgcttc tgcctgccgc
     1861 tctttcccca cgcgagcctg gccttcggcc ccctcgtgtt cacgctcgcc gtggtcggca
     1921 tcgtctacgg cgcgtgggtg tccaccgtgc agccggacct caagaagctg gtcgcgtact
     1981 ccagcgtgag ccacctcggc ttcgtgatgc tggggatctt cacgctgaac agccagggcc
     2041 tggtgggcgg cgtcatccag atgatcaacc acgggctcag cacgggggcg ctcttcctca
     2101 tggtcggcat gatctacgag cgccgacaca cccggctcat cgcggacttc ggggggctct
     2161 gggcggtgac gccggccttc tcggcgctgt tcctgatcgt cgtgctgtcc tcgctcgggc
     2221 tgccggggct caacggcttc gtgggcgagt tcctcatcct cgtcggtgct ttccgggtga
     2281 acggcctcct ggccgcggcg gcgacgacgg gcatcatctt cgccgccgtg tacctgctct
     2341 ggatgtatca gcgcgtggtc ttcggcccgc tgacccacga ggagaaccgt cgcctgcccg
     2401 acctgacgcc gcgggagtgg gccctcgtcg cgccgctcgt gctgctcatc gtctggatcg
     2461 gcgtgtaccc cgcgcccttc acgcggccca cggaggccac cgtcgaggcg ctgatcgcgc
     2521 aggtgcagac gaaggccgac gtcaccgtcg ccggggccgg cccgacccga ccccagctgg
     2581 cgcggccatg accggcgtcc ccgtgccgca agtcgccctc ggcgcgctgc tgccggcgct
     2641 catcgtcgtg gccacggccc tggtcgtcct gatgctcgac ctggtgccct cgcgcggcgg
     2701 caagacccac ctggccgtcg tcgcgctggc cggcgtcgtc ggcgcgctgc tggccacggt
     2761 gagcgcctgg ggcggcgccg gccgagcgtt ccgcgacatg gtggtgctcg acaactacgc
     2821 gctcttcttc cacgtcgtcg tctgctacgc ggcggcgctc gtgctgctgg tgtcggtgga
     2881 ctacctgcgg cgggcgggcg ccgaatcggg ggagtactac gcgctcgtcc tcttctcggc
     2941 gtcgggcatg ctactgctca cgtccgccaa cgatctcatc gtcgtgttcc tcgccgtcga
     3001 gctgatgtcg ctctcgctct acgtcctctc cggcctcttc aagcgccggc aggaggccgg
     3061 cgaggcgtcg atgaagtact tcctcctcgg cgtgttcgcc tccgccttcc tgctgtacgg
     3121 gatcgccctc gtgtacggcg ccacgggcac gacgaacttc gaccgcatcg ccgcggccgc
     3181 ggcggggccc cgcgatccgc tgctgctggt cggcctcgcg ctcctcctgg tcggcttcgg
     3241 cttcaagatc tcgtcggcgc cgttccacat gtgggcgccg gacgtctacg agggggcgcc
     3301 gacgagcgtc accgcgttca tcgccacggg ctcgaaggcg gcggtgttcg cggcgctgat
     3361 ccgtctggcc gtgaccgggg tccgcacctt ccaggccgac gcggcggcgc tcctgtgggg
     3421 actggccgcg gtcacgatga cggtcggcaa cgtcgtggcg atcgcgcagt cgaacctcaa
     3481 gcgcatgctc gcctactcct ccatcgcgca cgtcggctac atgctgatcg gcctcgtggt
     3541 cggcggcacg gcgggagcgg ctgcggtcct cttctatctc ctggcgtaca ccttcacgac
     3601 cgccggcgcc ttcggcgtga tcgccctctg cgagcggg
//
LOCUS       DATIAZ010000367         9952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1699543, whole genome shotgun sequence.
ACCESSION   DATIAZ010000367 DATIAZ010000000
VERSION     DATIAZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699543"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VLF19_12465"
     CDS             complement(<1..417)
                     /locus_tag="VLF19_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSE94114.1"
                     /translation="MTTLVRDVMTRAPLTIDPQAPVATAAAVMRERGFRHLPVVEDDG
                     RLVGMISDRDIRSACLAAAVAEYLPEEARRRRQALEALEETCVRHVMTWHVVTTHPQA
                     SVAQAAAEMLLAEVGSLAVVEHGRLVGIVTERDVLKA"
     gene            716..1162
                     /locus_tag="VLF19_12470"
     CDS             716..1162
                     /locus_tag="VLF19_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSE94115.1"
                     /translation="MKRIQRILHPSDFSPASAPAFATATEMAKARRAELILVHVLSPV
                     VPVVGDGYISPKAYDDLVRSTRAAGQKQLDSLLARARKAGVRGRALLLEGVTHDQIVR
                     AARSRRADLIVMGTHGRGGMAKLFLGSVAERVIGSARCPVLTVRKR"
     gene            1215..1568
                     /locus_tag="VLF19_12475"
     CDS             1215..1568
                     /locus_tag="VLF19_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94116.1"
                     /translation="MTIRRTSIAVGVLVVLAVTPAAAQRKSGPALDPQPEEPRMEAMM
                     KMMGEMRDEMQRMHAEMGRMQGMGAMSGRMGGMSARMGEMRGMMERHRQQMMKQCPAM
                     EAPARKPGEAPKPGS"
     gene            1572..2684
                     /locus_tag="VLF19_12480"
     CDS             1572..2684
                     /locus_tag="VLF19_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSE94117.1"
                     /translation="MSSSRIGAEALARYLAGVLGVEVRVLAVRPLSSKHADADDPKGF
                     GYGVPLEVECTVGGAPRSLVVARTRPAQGFGHDYPADRAWQALYGHTAYNGFPRHARS
                     LDVGFVRAGGELVSAADATEFFQLVEKIEGTLYWLDLDRLLEAPARDLDLARAEALGR
                     FLAEAHAVKRDEPTLYHRRIRELVGHGECLMGILDSYPHPYPLLPPAACAEIERAAVA
                     QRWRLRGRAHRLSRTHGDFHPWNLLFREGTDFSVLDRSRGDWGEPADDVAALGVNYLF
                     YGLRRRQGPAGDAGPFPRLFDAFLDAYLRASRDEEVREALPPFFAFRAIVIAHPLWYP
                     ELGDALRGALIAFAQRMMADTLFDAADVRALLEAAR"
     gene            2681..3277
                     /locus_tag="VLF19_12485"
     CDS             2681..3277
                     /locus_tag="VLF19_12485"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HSE94118.1"
                     /translation="MSWAIWVTGVPGSGKSAIARALAERLRATGEPVDVLELDVIRRA
                     LTPTPTYSDDEREVVYRALVYLARSLVEHGVPVIIDATAHRRAWRDLARASIAAFAEV
                     QLVCPLEVARERERTRHDSHAPPGIYAGATRPGATVPGVNVAYEPALAPEVTIDTSAE
                     EVDSAVARLLPLARELARRAPPSRGRRDACAGSARPAE"
     gene            3640..4752
                     /gene="ald"
                     /locus_tag="VLF19_12490"
     CDS             3640..4752
                     /gene="ald"
                     /locus_tag="VLF19_12490"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HSE94119.1"
                     /translation="MIVGVPKEIKPGEQRVAVTPAGARALIERGHEVLVEAGAGAGSG
                     IRDDDFARVGVRLTAVDDVWARAALVLKVKEPLPPEIERLRPGQVLFTYLHLAADHAL
                     TRALQKTDAIAVAYETVQRADGSLPLLTPMSEVAGRLAVQEGAFFLGRSRGGRGVLLA
                     GVPGVPRGNIAILGAGTVGLNAARTAVGLGADVSLLDVNLDRLRHAEEVFAGHVVTLY
                     STSLNVEQVVRRADLLVGAVLVPGARAPVLVSDAMVGTMKEGAVIVDVAVDQGGCVAT
                     MRPTTLAEPTYTVSGVVHYGVTNMPALVPRTSTFALTNATLPYALALADKGVARAARE
                     DPALALGVNVWRGRLVHRAVAEAAGELSAPLEDCLD"
     gene            complement(4801..6090)
                     /locus_tag="VLF19_12495"
     CDS             complement(4801..6090)
                     /locus_tag="VLF19_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HSE94120.1"
                     /translation="MTGSALLEARQLSKRFPLPGGGGEFTVLEKIDLALHEGEIVALL
                     GKSGSGKSTLLRILAGLIPATEGEVRYRGARVTGPCPGVAMVFQSFALLPWLDVLGNV
                     ELGLKARGVPAGVRLDTALRMVDVIGLDGFESAYPRELSGGMRQRVGIARALAVEPDI
                     LLMDEPFSALDVLSAESLRGELMDLWVGRKIPTRAILIVTHNIEEAVLLADRVVILGN
                     APGHVRGDLHVDLPRPRTREDPDVQAFSDELYQLLTRPEVAPEAARPEARAIPVPLPH
                     ARVGALAGFLEIVGERRGRVDLHRLAGELRLEVRELLPLVDAAALLGWVEVHEGDAIL
                     SVEGRAFVQAPVLARKELFRAALQQRPTLVTRIHAALAGKANRRLSESFFLGLLEQHF
                     SAVEARRQLDTAIDWGRYAELFGFEDDTNELFLESPD"
     gene            complement(6087..7808)
                     /locus_tag="VLF19_12500"
     CDS             complement(6087..7808)
                     /locus_tag="VLF19_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSE94121.1"
                     /translation="MTPRTFAPRLGAVGLNWADGAVFLALVGGLATLAWLGHGLWAPF
                     TPDAAPTLELDPWWLPYYAARSLLRMFAALGAALAFTLVVASWAARSARAGRVILPAL
                     DILQSVPVLGFLSATVTFFIAIVPGHVLGLELASIFAIFTSQVWNLTFAFHQVLVTLP
                     REQAEALRLYRVSGWQRFTHFELPAGIIPLVWNGMMSFGGGWFFLAASEAITVLNREL
                     LLPGVGSFMALAVKAADVRALGWAVLTMVVLILMVDAVVWRPLVAWSEKFKVEESQAT
                     DVPSSRVLDVLRRSHLVLWVGAGLARLDERLDRGWRRWLGAPGAGRPERRRWGRALTA
                     MTWTVIALLALRGAAFVMTALSGAEVVRVLGLGALTLLRVAGVVALAALVWTPIGTWI
                     GMNPRVARFAQPIVQVLASFPANFLFPLVALVFVRFGLSLEWGGALLMALGAQWYVLF
                     NTIAGATAIPNDLREMARNLGVRRVALWRALILPAIFPAWVTGGLTAAGGAWNASIVA
                     EVVTWGGTTLQATGVGAYVAAATAVGDWPRIVLGVAVMSAYVVALNRLVWRRLERLAV
                     ARYSLAA"
     gene            complement(7814..8881)
                     /gene="rtcA"
                     /locus_tag="VLF19_12505"
     CDS             complement(7814..8881)
                     /gene="rtcA"
                     /locus_tag="VLF19_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HSE94122.1"
                     /translation="MAGVLTLDGSRGEGGGQIVRTALALAVALGRRVAIERIRLRRPR
                     PGLQPQHLTVVRALAAISDAAVEGDALDSTAVSFAPRTTRAGEYRFDVGAVKGSAGSV
                     SLLLQALLLPLAGADRPSRLTLVGGTHVPWSPSVHYLTTVFLPALRALGVAADLTLRR
                     WGWYPAGGGEVEATIEPIRRVDALVVAAPATPVVLTGCSAVSRLPRSIAERQRCRAET
                     RLAAAGLKADIAIEEDRTAIGAGTMIFLGVPGRAGFSALGRRGLLAERVADAAVDALL
                     EWRESAAALDVHLADQLLPFLVLADGVSSFTCPEVSLHLRTVAWVVQRFVPAQIALLD
                     GRPARVEIHPAERRPSARSVE"
     gene            complement(8897..>9952)
                     /locus_tag="VLF19_12510"
     CDS             complement(8897..>9952)
                     /locus_tag="VLF19_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSE94123.1"
                     /translation="VGALDEDGYFLTDPEVVATLGVTPQDVERARGRVAREIERRMRL
                     YGTPPLARFLPDREVVFVDDGLATGLTMRAAVAYARRHGARRITIAVPCASAAAAERL
                     RQEADRVVSLAVDEAFTAVGAYYREFEPVSDDEVVAMLDRAGEAAQPRTDALRVSFEN
                     ARGLRLAGELLLPKAPGPHPVVVVAHGWAGSKGCPYDRAVADALVAAGVAAFLFDFSG
                     HGDSEGAPAASTRAQQVDDLRAALDVLDDVDDVTAARLAVAAEGSGAAASLRLAADDR
                     RIRALALCSPDAEGAETDVPRVTAPTLLVAGEHDEATRETCETLLPRLGGPGRLEVVP
                     LARAARLIADWLATHVR"
BASE COUNT         1409 a         3709 c         3614 g         1220 t
ORIGIN      
        1 cgccttgagg acgtcgcgct cggtgacgat gccgacgagg cgcccgtgct cgaccacggc
       61 caggctgccc acctctgcga gcagcatctc ggcggcggcc tgcgccacgg acgcctgcgg
      121 gtgggtcgtg acgacgtgcc acgtcatgac gtgccggacg caggtctctt ccagggcctc
      181 gagggcctgg cgccgccggc gcgcctcttc cggcagatat tcggcgacgg ccgccgcgag
      241 gcaggcgctc ctgatgtccc gatcgctgat catcccgacc agccggccgt cgtcctccac
      301 taccggtagg tgacggaagc cgcgctcgcg catgacggcc gccgcggtgg cgacgggcgc
      361 ctgcggatcg atcgtcagcg gcgccctggt catcacgtcc cgtacgagcg tggtcacgag
      421 gtcaccgggc ccttcggcgt cccgatgatg gcgatccggt ggcacatgcc cctaggggac
      481 gcaatggcgg tgccacgcct ggagcgcgat gattcgcgcg gttgctcgac gcctgcggca
      541 ccggcgacgg ggcgttgctg acccaagcgg ggaacggcag ccccagggcc gatcctgtcg
      601 gagatcccaa gtccgtgttt tgccgagctt cgcgtggtgg caatccggtt gcagcgcggg
      661 ccgagcggca tttcacgggc gccgacgggt cgtcggccga gagggaggtc gagcgatgaa
      721 gcgcattcag cggatcctgc acccgtcgga cttctctccc gcgtcggcgc cggccttcgc
      781 gacggcgacg gagatggcga aggcccggcg ggccgagctg atcctcgtgc acgtgctgtc
      841 gccggtcgtc cccgtggtcg gcgacgggta catctcgccc aaggcctacg acgacctcgt
      901 gcggtcgacc cgcgcggccg gccagaagca gctcgacagc ctcctggcgc gggcacgcaa
      961 ggccggcgtg cgcgggcggg cgctgctgct cgagggcgtc acgcacgacc agatcgttcg
     1021 cgccgcgagg tccaggcgcg cggacctcat cgtgatgggc actcacggac ggggcggcat
     1081 ggcgaagctc ttcctcggca gcgtcgccga gcgagtgatc ggcagcgccc ggtgcccggt
     1141 cctgaccgtc cgaaagcgat gagcggcggg cgccgggacc gcgccgatcg cgcgacacag
     1201 aggagaacga ggccatgacg atcaggagaa cctcgatcgc ggtgggcgtg ctcgtggtgc
     1261 tcgccgtcac ccccgccgcg gcccaacgca agagcgggcc ggccctggat ccgcagcccg
     1321 aagagccgcg gatggaagcg atgatgaaga tgatgggcga gatgcgcgac gagatgcagc
     1381 gcatgcacgc cgagatgggg cgcatgcagg gcatgggcgc catgtccggc cgcatgggcg
     1441 gcatgtcggc gcggatgggt gagatgcgcg gcatgatgga gcgccaccgg cagcagatga
     1501 tgaagcagtg cccggcgatg gaggcgcccg cgcggaagcc cggcgaggct ccgaagccgg
     1561 gcagctaggc cttgtcctct tccaggatcg gcgccgaggc gcttgcccgg tacctggcgg
     1621 gcgtcctcgg cgtcgaggtc cgcgtcctcg ccgtgcggcc gctgtcctcc aagcacgcgg
     1681 acgccgacga tccgaagggt ttcggctacg gcgtgcccct ggaggtcgag tgcaccgtgg
     1741 gcggcgcgcc gcgctcgctc gtggtggctc ggacgcggcc ggcacagggc ttcgggcacg
     1801 actatccggc cgaccgcgcc tggcaggcgc tctacgggca caccgcctac aacggcttcc
     1861 cgcgtcacgc caggagcttg gacgtcggct tcgtgcgcgc cggcggcgag ctggtctcgg
     1921 cggcggacgc caccgagttc ttccagctcg tggagaagat cgagggcacg ctctactggc
     1981 tcgatctcga ccgcctgctc gaggcgccgg cgcgcgacct cgacctcgcg cgggccgagg
     2041 cgctcggccg gttcctcgcc gaggcgcacg ccgtcaagcg cgacgagccc acgctgtacc
     2101 accgccggat ccgagagctg gtcggtcacg gcgagtgcct catgggcatc ctcgacagct
     2161 atccccaccc ctacccgttg ctgccgcccg ccgcgtgcgc ggagatcgag cgggccgccg
     2221 tcgcccagcg ctggcggctg cgcgggcgcg cgcaccggct gtcgcgcacg cacggcgatt
     2281 tccatccgtg gaacctgctg ttccgcgagg gcaccgactt ctcggtgctc gaccggagtc
     2341 gcggggactg gggggagccc gccgacgacg tggcggcgct cggcgtgaac tacctcttct
     2401 acggattgcg ccgccggcag ggacccgccg gcgacgccgg gccgttcccg cgcctgttcg
     2461 acgccttcct cgacgcctac cttcgcgcct cgcgcgacga ggaggtgcgg gaagcgctgc
     2521 cgccgttctt cgcattccgc gccatcgtca tcgcccatcc gctctggtac ccggagctcg
     2581 gcgacgcgct gcgcggcgcc ctcatcgcgt tcgcccagcg catgatggcc gacacgctct
     2641 tcgacgcggc cgacgtgcgg gcgctgctcg aggcggcacg atgagctggg cgatctgggt
     2701 caccggggtt ccgggcagcg gcaagtccgc catcgcccgg gcgctcgccg agcggctgcg
     2761 cgcgacgggc gagccggtcg acgtgctgga gctggacgtc atccgccggg ctctgacccc
     2821 gacgccgacc tacagcgacg acgagcggga ggtcgtgtac cgtgcgctcg tgtacctcgc
     2881 gcgctcgctg gtcgagcacg gcgtgccggt gatcatcgac gccaccgccc accgccgcgc
     2941 ctggcgtgac ctcgcgcgag cctcgattgc cgcctttgcg gaggtacagc tcgtctgccc
     3001 gctcgaggtc gcccgcgagc gcgagcgcac ccgccacgac agccacgcgc cgcccgggat
     3061 ctacgcaggc gcgaccaggc ctggcgccac ggtccccggc gtcaatgtcg cctacgagcc
     3121 cgcgctcgcg cccgaggtca cgatcgacac gtcggccgag gaagtcgaca gcgccgtcgc
     3181 gcgcctcctg cctctcgcgc gcgagctggc ccgccgggcg ccgccgtcgc gcggccggcg
     3241 ggacgcgtgt gccggaagcg cccggccggc cgagtagggg ccgctcggcg agatgatccg
     3301 ctcgggcgtc gtgctcgacg tcgcgggcgc catcatcatc tgggtggggc ggcggctgct
     3361 ctgcccgctc ttgggcgtga tgtgaccgcg gccccccctt cactcgtgct gctcgatctg
     3421 gtcgggccgc aagttacaag aatcgggcgc cacggcgacg gctcgcgcga ttcgcgccgc
     3481 gatccgacga tcgtggggac gggcgctggg accccggggg gtggcatacg acgtggccgg
     3541 ccgcgccggg catggtcgcg ccgtcgcctc ggcaaccgtc gacaacgcgg cgcccccgtg
     3601 ccacgatgga cggcgacggc gtgtcgggag gagtccactg tgatcgtcgg cgtgccgaag
     3661 gagatcaagc ccggcgagca gcgggtcgcc gtgacgccgg cgggcgcgcg cgcattgatc
     3721 gagcgcggtc acgaggtgct cgtcgaagcc ggcgccgggg ccggcagcgg gatccgcgac
     3781 gacgacttcg cccgcgtcgg cgttcgcctc acggcggtgg acgacgtctg ggcccgcgcc
     3841 gcgctcgtcc tcaaggtgaa ggagccgctg ccgccggaga tcgagcgcct gcgtccgggc
     3901 caggtcctgt tcacgtacct gcatctcgcc gccgaccacg cgctgacgcg agcgctccag
     3961 aagaccgacg ccatcgccgt cgcctacgag acggtccagc gcgccgacgg cagcctgccg
     4021 ctgctgaccc cgatgagcga ggtggccgga cgcctggccg tgcaggaggg cgcgttcttc
     4081 ctcggccgct cgcgcggcgg tcgcggcgtc ctgctcgccg gcgtgcccgg cgtgccccgc
     4141 ggcaacatcg ccatcctcgg cgccggcacc gtgggcctga acgccgcccg cacggccgtc
     4201 gggctcggcg cggacgtctc gttgctcgac gtgaacctcg accggctccg tcacgcggag
     4261 gaagtcttcg ccggccacgt cgtgacgctc tactcgacga gcctcaacgt cgagcaggtc
     4321 gtccggcgcg ccgatctcct cgtcggcgcc gtgctcgtgc ccggagcgcg ggcgcccgtc
     4381 ctggtgagcg atgccatggt ggggacgatg aaggagggcg cggtgatcgt cgacgtcgcc
     4441 gtggaccagg gcggatgcgt ggccacgatg cgccccacca cgctggccga gccgacctac
     4501 acggtgtccg gcgtcgtcca ctacggcgtg acgaacatgc cggccctggt gccgcgcacc
     4561 tcgacgttcg cgctcaccaa cgccacgctg ccctatgcgc tggcgctggc ggacaagggc
     4621 gtggcgcgcg cggcgcgcga ggatcccgcc ctcgccctcg gcgtgaacgt ctggcgcggc
     4681 cggctcgtgc accgcgcggt agcggaggcc gcgggcgagc tttccgctcc actcgaagac
     4741 tgcctcgatt aacagttggc gcacgaggcg ccagccggga gggggtcggg gtggcggggg
     4801 tcagtcgggg gattcgagga acagctcgtt ggtgtcgtcc tcgaagccga agagctcggc
     4861 gtagcggccc cagtcgatcg cggtgtcgag ctgccgccgg gcctccaccg cgctgaagtg
     4921 ctgctcgagg aggcccagga agaacgattc ggacagccgc cggttggcct tgcctgcgag
     4981 cgccgcgtgg atccgcgtca ccagcgtcgg acgctgctgg agcgccgcac ggaagagctc
     5041 cttgcgcgcg agcaccggcg cctggacgaa cgcgcggccc tcgacggaga ggatcgcgtc
     5101 gccctcgtgc acctcgaccc agccgagcag ggccgcggcg tccaccagcg gcagcagctc
     5161 gcggacctcc aggcgcagct cgccagccag ccgatgcagg tccacgcggc ctctccgctc
     5221 gccgacgatc tcgagaaagc ccgccagcgc gccgacccgc gcgtgcggca gcggcaccgg
     5281 gattgcccgg gcctccggcc gcgccgcctc cggcgcgacc tccggccgcg tgagcagctg
     5341 atacagctcg tcggagaagg cctggacgtc ggggtcctcg cgcgtccgcg gtcggggcag
     5401 gtcgacgtgg agatcgcccc ggacgtgccc gggggcgttg cccaggatca cgacgcggtc
     5461 ggccagcagc accgcctcct cgatgttgtg ggtcacgatg aggatcgcgc gggtggggat
     5521 cttccgtccc acccacaggt ccatgagctc gccgcgcagg ctctccgccg agagcacgtc
     5581 cagcgccgag aacggctcgt ccatcaggag gatgtctggc tccacggcga gggcccgcgc
     5641 gatccccacg cgctgccgca tcccgcccga cagctcgcgc gggtaggcgg actcgaaccc
     5701 gtcgagcccg atgacgtcga ccatccggag cgccgtgtcc agccgcacgc cggccggcac
     5761 gcccctggcc ttgagaccca gctcgacgtt gccgaggacg tcgagccacg gcaacagcgc
     5821 gaagctctgg aagaccatgg ccacgcccgg gcagggcccg gtcaccctgg ccccgcgata
     5881 gcgtacctcg ccctcggtgg ccgggatgag gccggcgagg atccgcagca gcgtggactt
     5941 gcccgagccc gacttgccga ggagcgcgac gatctcgccc tcgtgcagcg cgaggtcgat
     6001 cttctcgagc accgtgaact ccccgccacc gccgggcagc gggaagcgct tgctcaactg
     6061 ccgggcctcg aggagcgcgc tccccgtcat gccgcgaggc tgtagcgggc gacggccagc
     6121 cgctcgagcc ggcgccacac gagccggttc agggcgacca cgtaggcgct catgaccgcc
     6181 acgccgagca cgatgcgcgg ccagtcgccg acggcggtcg ccgcggcgac gtacgccccg
     6241 accccggtgg cctggagcgt cgtgcccccc caggtcacca cctcggccac gatgctcgcg
     6301 ttccaggcgc cgcccgccgc ggtgaggccg cccgtcaccc aggccgggaa gatcgcgggc
     6361 aggatcagcg cgcgccagag cgccacgcgg cggacgccga ggttgcgcgc catctcgcgc
     6421 aggtcgttcg gaatcgccgt cgcgcccgcg atcgtgttga agaggacgta ccactgtgcg
     6481 ccgagcgcca tgaggagcgc cccgccccat tccaggctca gcccgaaccg gacgaagacg
     6541 agggcgacca gcggaaacag gaagttcgcg ggaaacgagg ccagcacctg gacgatcggc
     6601 tgcgcgaacc gggcgacgcg cgggttcatg ccgatccacg tgccgatcgg cgtccagacg
     6661 agcgcggcca gcgcgaccac ccccgccacc cgcagcaggg tcagcgcgcc gaggccgagc
     6721 accctgacca cctcggcgcc ggagagcgcc gtcatgacga aggcggcccc gcgcagcgcg
     6781 agaagggcga tgacggtcca ggtcatggcc gtgagcgcac gcccccaccg gcgccgctcc
     6841 ggccgcccgg cgccgggggc gccgagccag cgccgccacc cgcggtcgag ccgctcgtcg
     6901 aggcgagcca ggcccgcccc gacccacagc accagatggg agcgccggag cacgtcgagc
     6961 acgcgcgagc tcggcacgtc cgtggcctgt gactcctcga ccttgaactt ctccgaccag
     7021 gcgaccagcg gccgccagac gacggcgtcg accatcagaa tcaggaccac catggtgagc
     7081 accgcccagc ccagggcccg gacgtctgcc gccttcacgg ccagcgccat gaacgagccg
     7141 acgccgggga gcagcagttc gcggttcagc accgtgatcg cctcgctcgc ggccaggaag
     7201 aaccagccgc ccccgaacga catcatgccg ttccagacga gcgggatgat tcccgccggc
     7261 aactcgaagt gggtgaaccg ctgccagccc gagacgcggt agagccggag cgcttcggcc
     7321 tgctcgcgcg gcagcgtgac gagcacctgg tggaacgcga acgtgagatt ccacacctgg
     7381 ctcgtgaaga tcgcgaagat cgacgccagc tcgaggccca ggacgtgtcc cggcacgatc
     7441 gcgatgaaga aggtcaccgt cgccgacagg aagccgagca ccggcacgga ctggagaatg
     7501 tcgagggccg gcaggatcac gcgcccggcc cgcgccgacc gggccgccca ggaggcgacg
     7561 accagcgtga aggcgagcgc cgccccgagc gcggcgaaca tgcgcaggag cgaccgcgcg
     7621 gcgtagtacg gcagccacca ggggtcgagc tcgagcgtcg gtgcggcgtc cggcgtgaac
     7681 ggcgcccaga ggccgtggcc gagccacgcg agcgtggcga ggccgcccac cagcgccagg
     7741 aacaccgcac cgtcggccca gttcaggccg acggcaccga gccgaggggc gaacgtccgc
     7801 ggcgtcatcg cggtcactct acgcttcgcg cggaaggacg acgttccgcc ggatggatct
     7861 cgacccgggc ggggcggccg tcgaggaggg cgatctgcgc gggcacgaac cgctggacga
     7921 cccaggcgac cgtgcgcaga tggagcgaca cctccgggca cgtgaacgac gagacgccgt
     7981 cggccagcac gaggaacggc aacagctgat cggccaggtg gacgtccagc gcggcggcgc
     8041 tctcccgcca ctcgagcagc gcgtccaccg ccgcgtcggc gacccgctcg gcgaggagac
     8101 cccgccggcc gagggccgag aagccggccc gccccgggac gccgagaaag atcatcgtgc
     8161 cggcgccgat cgccgtgcga tcctcctcga tcgcgatgtc cgccttcagg cccgccgccg
     8221 ccaggcgcgt ttcggcccga caccgctggc gctccgcgat cgagcggggc agccgcgaga
     8281 ccgcggagca cccggtgagg acgaccggcg tcgcgggggc agccaccacg agcgcgtcga
     8341 ctcgccggat cggctcgatc gtggcctcca cctccccgcc gccggccggg taccagcccc
     8401 accggcgcag cgtcaggtcc gccgcgacgc cgagggcgcg cagcgccggc aggaacaccg
     8461 tcgtgaggta gtgcacgctc gggctccacg gcacgtgcgt gccgccgacg agcgtcagcc
     8521 gcgagggtcg atcggcgccg gccagcggga gcagcagcgc ctggaggagc agcgacaccg
     8581 atcccgcgct gcccttgacg gcgccgacat cgaaccggta ctcgccagcc cgggtcgtcc
     8641 gcggcgcgaa cgacaccgcc gtcgagtcga gcgcgtcgcc ctcgacggcg gcgtcgctga
     8701 tcgcggcgag cgcgcggacg accgtgaggt gctgcggctg gagccccggc ctcgggcgac
     8761 gcaggcggat gcgctcgatc gccacccggc gccccagggc caccgcgagc gcgagcgccg
     8821 tgcggacgat ctggcccccg ccctcaccgc gcgatccgtc gagggtcagc acgcctgcca
     8881 cgccgccctc gagcgctcag cgcacgtgcg tggcgagcca gtcggcgatc agacgcgccg
     8941 ctcgggccag cgggacgacc tcgaggcgcc cggggccgcc caggcggggc aggagcgtct
     9001 cgcacgtctc ccgggtcgcc tcgtcgtgct cacccgccac gagaagcgtc ggggcggtca
     9061 cccgtggcac gtcggtctcg gcaccctccg cgtccggtga gcacagggcg agcgcgcgga
     9121 tccgtctgtc gtccgcggcg agccgcagcg acgcggccgc gccggagcct tctgcggcga
     9181 cggccagtcg cgcggccgtc acgtcgtcga cgtcgtcgag cacgtcgagc gcggcgcgca
     9241 ggtcgtccac ctgctgtgcc cgggtgctcg ccgccggcgc cccctcgctg tcgccgtgcc
     9301 cgctgaagtc gaacaggaag gccgccacgc cggcggcgac gagggcgtcg gccacggccc
     9361 ggtcgtacgg gcagcccttg ctgccggccc agccgtgcgc gaccaccacg accgggtgcg
     9421 ggccgggcgc cttcggtaac agcagctcgc ccgccagccg gagcccgcgc gcgttctcga
     9481 acgacacgcg cagcgcgtcc gtccgcggct gcgcggcctc tcccgcgcga tcgagcatcg
     9541 ccaccacctc gtcgtccgac acgggctcga actcccggta gtatgcgccg acggccgtga
     9601 aagcctcgtc gacggcgagg ctcacgaccc gatccgcctc ctggcgaagc cgctcggccg
     9661 ccgctgccga ggcgcagggc accgcgatcg tgatccgccg cgccccgtgg cggcgcgcgt
     9721 atgccaccgc cgcacgcatg gtgaggccag tggccagtcc gtcgtcgacg aagacgacct
     9781 cgcgatcggg caggaagcgc gcgagcggcg gcgtcccgta cagccgcatc cgccgctcga
     9841 tctcccgggc gacgcgaccg cgcgcccgct cgacgtcctg cggcgtgaca ccgagcgtgg
     9901 cgaccacctc cggatcggtc agaaaatacc cgtcctcgtc gagcgcgccc ac
//
LOCUS       DATIAZ010000368         5244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1568141, whole genome shotgun sequence.
ACCESSION   DATIAZ010000368 DATIAZ010000000
VERSION     DATIAZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568141"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /gene="tuf"
                     /locus_tag="VLF19_12515"
     CDS             <1..140
                     /gene="tuf"
                     /locus_tag="VLF19_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589624.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSE94124.1"
                     /translation="MVMPGDNVTMAIELIVPIAMEKELRFAIREGGRTVGAGVVTEIT
                     E"
     gene            144..293
                     /gene="rpmG"
                     /locus_tag="VLF19_12520"
     CDS             144..293
                     /gene="rpmG"
                     /locus_tag="VLF19_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HSE94125.1"
                     /translation="MRDIISMACTTCQRRNYTTTKNKRTHPDRLELKKFCRWCRKHTP
                     HKESK"
     gene            446..522
                     /locus_tag="VLF19_12525"
     tRNA            446..522
                     /locus_tag="VLF19_12525"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Trp,seq:cca)
     gene            559..750
                     /gene="secE"
                     /locus_tag="VLF19_12530"
     CDS             559..750
                     /gene="secE"
                     /locus_tag="VLF19_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSE94126.1"
                     /translation="MGVIAKVREFTREVMVEFRKVTWPSRPELVNSTTVVVVVTVVLA
                     LFLGAVDIALARIVERILR"
     gene            747..1301
                     /gene="nusG"
                     /locus_tag="VLF19_12535"
     CDS             747..1301
                     /gene="nusG"
                     /locus_tag="VLF19_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSE94127.1"
                     /translation="MSGETATAAPKQWFVVHTYSGFENKVAEAIQSRAKIFNLGEKIG
                     RVLVPTEKVVEVRNKQKRETEQKFFPGYVLVEMELTDDTWQLVRATPKVTGFVGSGAR
                     PVALTGDQVDEIMRQMEAGAEKPKPKSVFQRGDKVRVIDGPFVNFQGVVEDINPERGR
                     MKVVVPVFGRPTPVELEYYQVERL"
     gene            1328..1747
                     /gene="rplK"
                     /locus_tag="VLF19_12540"
     CDS             1328..1747
                     /gene="rplK"
                     /locus_tag="VLF19_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSE94128.1"
                     /translation="MKKVQAMVKLQIPAGKANPSPPVGPALGQHGVNIMEFCKGFNAQ
                     TQSQEGLILPVVVTIYQDRSFTFVVKTPPAAVLLKRAAGIAKASAVPHKDKIGRVTKQ
                     QVREIAQTKLVDLNTDNIDAAMRIVEGTARSMGIDVI"
     gene            1759..2490
                     /gene="rplA"
                     /locus_tag="VLF19_12545"
     CDS             1759..2490
                     /gene="rplA"
                     /locus_tag="VLF19_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSE94129.1"
                     /translation="MATVVEKKAGKRYDAGTAKLEAGREYSIEEAVAVLKEMPAAKFN
                     ESVDLSLRLGVDPKHADQMVRGAVVLPHGIGKTVRVAVFAKGEKEREAREAGADVVGA
                     EDLVEKVQGGFMEFDTAVATPDLMGQVGRLGKVLGPRGLMPNPKLGTVTFDVARAVRD
                     AKAGKVEFRVDKAGNVHVPIGKRSFPVEQLAANALAIIEAIVRAKPAAAKGTYLRSLT
                     LSSTMGPGVAIDTQRTANLFKKQQA"
     gene            2518..3054
                     /gene="rplJ"
                     /locus_tag="VLF19_12550"
     CDS             2518..3054
                     /gene="rplJ"
                     /locus_tag="VLF19_12550"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSE94130.1"
                     /translation="MPTQEKVTAVEDLKGRLDGAKTVVLAEYRGLTVRQLSELRKQLR
                     AVSAQYKVVKNRLAKIAIASTELEGLAKHLNGPTGMIVSKEDPVAVAKALQTFARTNQ
                     ALAIKAGVVDGQVLEPAGLKALADLPSREALRAQIVGAVQGPLAQLVGLLSTPQRELA
                     YVLAQRGKDAADTNGEDA"
     gene            3080..3466
                     /gene="rplL"
                     /locus_tag="VLF19_12555"
     CDS             3080..3466
                     /gene="rplL"
                     /locus_tag="VLF19_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSE94131.1"
                     /translation="MPTIEEIADQLDKLSLLEAANLSKLLQERWGVSAAAPVAVAAVP
                     GAAGAAAAGAAAEEKTEFDVILSAAGDKKIQVIKVVRELTGLGLKEAKDLVDGAPKPV
                     KEKVTKAEAEDMKKKLEEQGAAVQVK"
     gene            3576..>5244
                     /locus_tag="VLF19_12560"
     CDS             3576..>5244
                     /locus_tag="VLF19_12560"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSE94132.1"
                     /translation="MAGVIQCGRRSRKDFGKIPSIVEIPNLIEVQKRSYETFLQKDVP
                     ADRREDIGLQAVFKSVFPIADYNDNALLEFDSYHFGEPKYTVEECHDRGMTFAIPLKV
                     TLRLVVFDHDKEAKTRTIREQRGQEVYLGELPLMTEKGTFIINGTERVVVSQLQRSAG
                     VFFDDDKGKTLASGKPLYSARVIPYRGSWVEFDFDANDVLHVRVDRRRKMLATAFLRA
                     FWFLEKGVILSDEEILGQFYETEDVQGFEGGNAWVKLHPEAHVGAKVAEDVKPPRHRE
                     PIVTAGKVLNAKLVEKMVEAGVDRIAVRAESVVGRRTASRIVDTESGEVLVETNTEIT
                     STVLSQLMSRRVAPFKLLVVASGKVDASIYETLGKDQFKNPDEALVEIYRRLRPGDPP
                     TIDSARALFRGMFMDARRYDLARVGRFMINKKLGIDAPLHIKTLRSEDVVSVIRHLLM
                     VKLGSKPTDDIDHLGNRRVRSVGELLENQFRVGLTRMERAVKERMSISDIAALMPHDL
                     INAKPVSAVVKEFFGSSQLSQFMDQTNPLAELTHKRRLSALGPRGLSR"
BASE COUNT          969 a         1647 c         1885 g          743 t
ORIGIN      
        1 agatggtgat gccgggggac aacgtgacga tggcgatcga gctgatcgtg ccgatcgcga
       61 tggagaagga gctgcggttt gcgatccgcg aggggggccg cacggtgggc gccggcgtgg
      121 tgacggagat cacggagtag gccatgcgcg acatcatctc gatggcctgc accacgtgcc
      181 agcgccgtaa ctacacgacg acgaagaaca agcgcaccca ccccgaccgt ctcgagctca
      241 agaaattctg ccgctggtgc cgcaagcaca cgccgcacaa agagtcgaag tagtgcgcag
      301 ggcgaaggcg gttcatcgga aggaagacgg tgcgaccgga acgcttgaag acgtggcgag
      361 actggaaatg gcacttcgag cgcgggcgtt gtccgcgcaa tcgattcgga gattcggaga
      421 gagccgtcgg ggcactctcc gaggtagggg tgtagctcta acggctagag caccggactc
      481 caaatccggg ggatgggggt tcgaatccct ccacccctgc cacttatcga cggcacgagg
      541 ggcggtcgag gcgagacgat gggtgtgatc gcgaaggtcc gcgagttcac gcgcgaagtc
      601 atggtggagt tccgcaaggt gacgtggccc agccgtcccg agctggtcaa ctccacgacg
      661 gtggtggtcg tggtgaccgt cgtgctggcc ctgttcctgg gggccgtgga catcgcgctg
      721 gcgcgcatcg tggagaggat cctgagatga gcggcgagac ggcgacggcg gcgcccaagc
      781 agtggttcgt cgtccacacg tattccgggt tcgagaacaa ggtcgcggag gcgatccagt
      841 cgcgggcgaa gatcttcaac ctcggggaga agatcggccg cgtgctggtg ccgaccgaga
      901 aggtggtcga ggtccgcaac aagcagaagc gcgagaccga gcagaagttc ttccccgggt
      961 acgtgctggt ggagatggag ctgacggacg acacgtggca gctcgtgcgc gcgacgccca
     1021 aggtcaccgg cttcgtgggg tcgggggcgc ggccggtcgc gctcacaggc gaccaggtcg
     1081 acgagatcat gcgccagatg gaggcgggcg ccgagaagcc caagcccaag tccgtcttcc
     1141 agcggggtga caaggtgcgc gtcatcgatg gcccgttcgt gaacttccag ggcgtcgtcg
     1201 aggacatcaa ccccgagcgc gggcgcatga aggtggtcgt gccggtgttc ggccggccga
     1261 ccccggtcga gctcgagtac taccaggtgg agagactctg atgggcgtct ctggcggggc
     1321 gtcatagatg aagaaggtcc aggcgatggt gaagctgcag atcccggccg gcaaggccaa
     1381 tccctcgccg ccggtggggc cggcgctcgg ccagcacggc gtgaacatca tggaattctg
     1441 caagggcttc aatgcccaga cgcagagcca ggaaggcctc atcctgccgg tggtcgtgac
     1501 catctaccag gaccggtcct tcacgttcgt cgtcaagacg ccgccggcgg ccgtgctgct
     1561 caagcgcgcg gccggcatcg cgaaggcctc ggcggtgccc cacaaggaca agatcggcag
     1621 ggtgacgaag cagcaggtgc gcgagatcgc gcagaccaag ctcgtggacc tcaacaccga
     1681 caacatcgac gccgcgatgc gcatcgtcga gggcaccgcc cgcagcatgg gcatcgacgt
     1741 catctaggag agcggagcat ggccaccgtg gtcgagaaga aggccggcaa gcgctacgac
     1801 gccggcacgg cgaagctcga ggcgggacgc gagtactcca tcgaggaggc ggttgcggtg
     1861 ctcaaggaga tgccggcggc gaagttcaac gagtcggtcg acctgtcgct ccgcctgggc
     1921 gtcgatccca agcacgccga tcagatggtg cgcggtgccg tggtgctgcc gcacggcatc
     1981 ggcaagaccg tccgggtggc cgtcttcgcc aagggcgaga aggagcgcga ggcccgcgag
     2041 gccggcgccg acgtcgtggg cgcggaggat ctcgtcgaga aggtgcaggg cggcttcatg
     2101 gagttcgaca ccgccgtcgc cacaccggac ctcatgggcc aggtcggtcg gctcggcaag
     2161 gtgctgggtc cccgcggact catgcccaac ccgaagctcg gcacggtgac gttcgatgtc
     2221 gcccgcgctg tgcgcgacgc gaaggccggc aaggtcgagt tccgggtgga caaggccggc
     2281 aacgtccacg tgcccatcgg caagcgctcg ttcccggtag agcagctggc cgccaacgcg
     2341 ctggccatca tcgaggccat cgtccgcgcg aagcctgcgg ccgcgaaggg gacgtacctc
     2401 cggtcgctca cgctgtcgtc gacgatgggg cccggcgtcg ccatcgacac ccagcggacg
     2461 gcgaacctgt tcaagaagca gcaggcgtag ccgaccacga ggagggggaa agccaccgtg
     2521 ccgacgcagg agaaggtcac tgcggtcgag gatctcaagg gacgtctgga cggcgccaag
     2581 acggtggtgc tggccgagta ccgcggcctg accgtccggc agctcagcga gctccgcaag
     2641 cagctccggg ccgtctcggc ccagtacaag gtcgtcaaga accgtctggc gaagatcgcc
     2701 attgcatcga ccgagctcga ggggctcgcc aagcacctga acggtccgac ggggatgatc
     2761 gtctcgaaag aggaccccgt cgccgtcgcc aaggcgctgc agacgttcgc gaggaccaac
     2821 caggcgctgg ccatcaaggc gggcgtggtc gatggccagg tgctggagcc agcgggcctg
     2881 aaggcgctgg ccgacctgcc gtcgcgcgaa gcgttgcgcg cgcagatcgt gggcgcggtt
     2941 caggggccgc tggcccagct cgtgggcctc ctgagcacgc cgcagcgcga gctggcctac
     3001 gtgctcgcgc agcggggcaa ggacgccgcg gacaccaacg gggaagacgc gtaggtcgcc
     3061 cggacaaagg agacaatgga tgccgaccat cgaagagatt gctgatcagc tcgacaagct
     3121 ttcgctgctg gaagcggcga acctctccaa gctgctccag gagcgctggg gtgtgtcggc
     3181 cgcggcgccg gtcgcggtcg ccgcggtccc gggcgcggcg ggtgccgccg ccgcaggcgc
     3241 cgccgccgag gagaagacgg agttcgacgt gatcctgtcc gcggccggcg acaagaagat
     3301 ccaggtgatc aaggtcgtcc gggagctgac gggcctgggc ctcaaggagg ccaaggacct
     3361 cgtggacggc gcgccgaagc ccgtcaagga gaaggtgacg aaggccgagg ccgaggacat
     3421 gaagaagaag ctggaggagc agggcgccgc ggtgcaggtg aagtgagctc ggcctcccca
     3481 ggacgggcag ggattgcgcc ggtaaagccg gcgcgcgagg cgaagcaaca ccatcgcgtt
     3541 gaggacggcc cggagggccg tcctcgggag acgatatggc aggagtgatt cagtgcgggc
     3601 gccgcagccg caaggacttc ggcaagatcc cgtccatcgt cgagattccg aacctgatcg
     3661 aggttcagaa gcgctcgtac gagaccttcc tccagaagga cgtgccggcg gaccggcgcg
     3721 aggacatcgg cctgcaggcg gtgttcaagt cggtctttcc gatcgccgac tacaacgaca
     3781 acgcgctgct cgagttcgac tcgtaccact tcggcgagcc caagtacacg gtcgaggagt
     3841 gccacgaccg cggcatgacc ttcgcgatcc cgctcaaggt cacgctccgc ctcgtcgtgt
     3901 tcgaccacga caaggaggcg aagacccgca ccatccgcga gcagcgcggt caggaggtct
     3961 acctgggcga gctgccgctc atgaccgaaa agggcacgtt catcatcaac ggcaccgagc
     4021 gcgtggtcgt ctcgcagctg cagcgctcgg ccggcgtgtt cttcgacgac gacaagggca
     4081 agacgctggc gtccggcaag ccgctctact cggcgcgggt catcccctac cgcggctcct
     4141 gggtggagtt cgacttcgac gccaacgacg tgctgcacgt gcgtgtggac cggcgccgca
     4201 agatgctggc cacggcgttc ctgcgcgcct tctggttcct cgagaagggc gtcatcctgt
     4261 ccgacgagga gatcctgggc cagttctacg agaccgagga cgtgcagggc ttcgagggcg
     4321 gcaacgcgtg ggtgaagctc caccccgagg cccacgtggg ggccaaggtg gccgaggacg
     4381 tcaagccgcc gcgccaccgc gagccgatcg tcaccgccgg caaggtgctc aacgccaagc
     4441 tcgtcgagaa gatggtcgag gccggcgtgg accggatcgc cgtccgggcc gagtccgtcg
     4501 tgggccgccg gacggcctcg cgcatcgtcg acaccgagag cggcgaggtg ctcgtcgaga
     4561 ccaacacgga gatcacctcc acggtgctct cgcagctgat gagccggcgg gtggctccgt
     4621 tcaagctcct ggtcgtcgca tccggcaagg tggacgcctc gatctacgag acgctcggca
     4681 aggaccagtt caagaacccg gacgaggcgc tggtggagat ctaccggcgg ctgcggccgg
     4741 gcgacccgcc gaccatcgac tctgcccgcg cgctgttccg cggcatgttc atggacgcgc
     4801 ggcgctacga tctggcgcgg gtcggccggt tcatgatcaa caagaagctc ggcatcgacg
     4861 ctccgctgca catcaagacg ctgcgcagcg aggacgtggt gtccgtcatc cggcacctgc
     4921 tgatggtgaa gctcggcagc aagcccaccg acgacatcga ccacctcggc aaccggcggg
     4981 tgcggtcggt gggcgagttg ctcgagaacc agttccgcgt gggcctcacc cgcatggagc
     5041 gcgcggtgaa ggagcgcatg tccatctcgg acatcgcggc gctcatgccc cacgatctca
     5101 tcaacgccaa gccggtctcg gcggtggtga aggagttctt cggctcctcg cagctgagcc
     5161 agttcatgga ccagaccaac ccgctcgccg agctgaccca caagcgccgc ctgtccgcgc
     5221 tgggcccgcg cgggctgtcg cggg
//
LOCUS       DATIAZ010000369         4791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_252985, whole genome shotgun sequence.
ACCESSION   DATIAZ010000369 DATIAZ010000000
VERSION     DATIAZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252985"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VLF19_12565"
     CDS             complement(<1..798)
                     /locus_tag="VLF19_12565"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HSE94133.1"
                     /translation="MNSPGDPSGPGSFRRGGDRSTGGRHAGPAPAADTPPTPEPTFAE
                     RARTLLHQGRAGTLATLARRPPGHPFASLMPYAPDADGQPLLLISSMAVHTQNLAGDP
                     RASLLVTQPDMSGEPLAGGRVTLMGEGRRLPEGEVGVARETYLARQANAAYWADFEDF
                     AFFRLEVTDVYFVGGFAAMGWVDADEYRAARPDPLADAASSILEHMNRDHAGALIEYA
                     RFFAGAEAEEATMAAVDRLGFKLRLRQGHRRWSVRIGFPRGVTSAGET"
     gene            complement(795..1238)
                     /gene="trxA"
                     /locus_tag="VLF19_12570"
     CDS             complement(795..1238)
                     /gene="trxA"
                     /locus_tag="VLF19_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSE94134.1"
                     /translation="MATELRCPACGAVNRVPEARAGQPARCGKCKAPLATTIAPVDVT
                     DATFEATVARASAPVLLDCWADWCGPCHALAPTIDALARAWAGRAIVGKLDVDASPAT
                     ASRFEVRSIPTLLLFKDGRLVDRLVGVPPRAAIEARLQALAGPAA"
     gene            complement(1282..4248)
                     /locus_tag="VLF19_12575"
     CDS             complement(1282..4248)
                     /locus_tag="VLF19_12575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSE94135.1"
                     /translation="MAVDRILEGLNEAQREAVTHDVGPLLIVAGAGTGKTSVITRRIA
                     WLIAQRRARPEEILALTFTDKAAAEMEERVDTLVPYGYADVEISTFHAFGDRILREHA
                     LEIGLTPDFRVLNRAEQVIFVRDRLFDLPLEHYRPLGDPTRHVQALITLISRCKDEDV
                     SPEAYLAYAERLAREAPAAPDYEEARERAAQQRELAATYAKCQELMAREGAVDFGDQI
                     VHVLRLLRARPYVLSAYQRRFKYIMVDEFQDTNHAQFELVKLLAARHGNVACVGDEDQ
                     AIYRFRGAAVSNILGFLDRYPEARQVVLTENYRSHQASLDAAYRLIVHNNPDRLEVRR
                     GISKRLVAARAVTGSPPVHLHYETGTQEAEAVAAMIRERVGVGAWKHDDVAILVRSND
                     DADQFLRSLNLRSIPWTFSGNAGLYGRPEIRLLIAFLRAVVHLDESVSLHYLASSDLY
                     QVPIADLTRCSTYADRKHVHLFDVLRRTGEIRDLRDEIDEAGHAAIGHLVADLERYME
                     LGRETPTGELLYLFLKDSGWLGRMYREETARDVAETKNIAKFFDRVKSASQALRYDNV
                     REFVKHLDALIEAGEDPAVAEADLETPAVRVLTVHKAKGLEFPVVFLVNLVHEKFPSR
                     RRRDALELPVALVKDALVSGDVHVQEERRLFYVGMTRARHELYLTSAADYGGTRRRKV
                     SEFVLEALDLPRDAARPFKARPVEAIEHFAPSLAADDSALQPLAPDAELVVSHKQIDD
                     YQTCPLKYLNVHVRRIPIRRHHAVAYGAVVHKVVEYYLRRRAVGNYTPLDDLLAVYDR
                     AWAGEDILHDRPGSHEPAEGFLTREHEEARRAAGRDALKRFWHQEEADGVKPTWIEKE
                     FGFALGLDRVRGRYDRVDEDLLGAVIIDYKTSDVRRQKDADRRVAGSLQLKMYALAWR
                     EMTGGLPQRVELRFIDSSVVGRHVPTGDDCEEAIDAVKAAAAGIRARRFDATPSFGAC
                     RQCAYNQICPYTATSE"
     gene            4279..>4791
                     /locus_tag="VLF19_12580"
     CDS             4279..>4791
                     /locus_tag="VLF19_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94136.1"
                     /translation="MRLTIRAKLVLLSLPILVVVSFGFMLFQQRLSRGWVEEDLRERA
                     IVFAREMAATIGDRREFESGELLARQIGQIMDVRPSVLQLDVLAFDATGSRVMATSHP
                     LSRLPVTRRDADQVRRGRAVSRLVDAERARYWEVVAPVALDGGVVGAVAAKFSLARAD
                     ALAARIGTAAL"
BASE COUNT          649 a         1672 c         1762 g          708 t
ORIGIN      
        1 ggtctcgccg gcgctcgtca ccccgcgtgg aaatccgatg cggacgctcc accgccggtg
       61 gccctgccgg agccggagct tgaagccgag gcgatcgacg gcggccatcg tggcctcctc
      121 ggcctcggcg ccggcgaaga agcgcgcata ctcgatgagc gcgccggcgt ggtcccgatt
      181 catgtgctcg aggatggagc tcgcggcgtc cgcgagcggg tcgggccggg ccgcgcggta
      241 ctcgtcggca tccacccagc ccatcgccgc gaagccgccg acgaagtaga cgtcggtgac
      301 ctccaggcgg aagaaggcga agtcctcgaa gtccgcccag taggcggcgt tcgcctgccg
      361 cgccaggtag gtctcgcgcg ccaccccgac ctcgccctca ggaagccggc ggccctcacc
      421 catcagcgtg acgcggccgc cggccagcgg ctcaccggac atgtcgggct gcgtgacgag
      481 caggctcgcg cgggggtcac cggcgaggtt ctgggtgtgc acggccatcg acgagatcag
      541 cagcaggggc tgcccgtcgg cgtcgggcgc gtagggcatg agcgacgcga agggatggcc
      601 cgggggcctg cgggcgagcg tggccagcgt cccggcgcga ccctgatgca ggagggtccg
      661 agcgcgctcg gcgaacgtcg gctcgggagt gggcggtgtg tccgccgccg gcgccggccc
      721 ggcgtgacgt ccgccagtgc tccggtcgcc cccgcgacgg aaggagcccg gcccgctcgg
      781 atcgcctggc gagttcaagc cgcgggcccg gcgagcgcct gcaggcgtgc ctcgatcgcc
      841 gcccggggcg gcacgccgac gagtcgatcc accagccggc cgtccttgaa cagcagcagc
      901 gtcgggatgc tccggacctc gaagcgggaa gcggtcgccg gactcgcgtc gacgtcgagc
      961 ttgccgacga tcgcgcgtcc ggcccaggcg cgggcgagcg cgtcgatggt cggggccagc
     1021 gcgtggcacg ggccgcacca gtcggcccag cagtcgagca gcaccggcgc gctcgcccgg
     1081 gccaccgtcg cctcgaacgt ggcgtcggtc acgtcgaccg gcgcgatcgt cgtggccagc
     1141 ggcgccttgc acttaccgca gcgcgccggc tgtccggcac gcgcctcggg cacgcggttg
     1201 acggctccgc acgcgggaca gcgcaactcc gtggccatga ggtcagtgta cgcgggctga
     1261 caaccgcgcc ggagacgacg gctactcgct ggtcgcggtg tacgggcaga tctgattgta
     1321 ggcgcactgc cggcaggcgc cgaacgacgg cgtggcgtcg aagcggcggg cgcggatgcc
     1381 agcggccgcc gccttgacgg cgtcgattgc ctcctcgcag tcgtcgccgg tcggcacgtg
     1441 gcggcccacg acgctggagt cgatgaagcg cagctcgacg cgctggggca ggccgcccgt
     1501 catctcccgc cacgccagcg cgtacatctt gagctggagg ctcccggcca cccggcggtc
     1561 ggcgtccttc tggcgcctga cgtcgctcgt cttgtagtcg atgatcaccg cgcccaggag
     1621 gtcctcgtcc acgcggtcgt agcggccgcg cacgcggtcg agccccagcg cgaagccgaa
     1681 ctccttctcg atccaggtcg gcttcacgcc gtccgcctcc tcctggtgcc agaagcgctt
     1741 gagcgcgtcg cggccggccg cccggcgcgc ttcctcgtgc tcgcgggtga gaaagccctc
     1801 ggccggctcg tggctgccgg ggcggtcgtg caggatgtcc tcgccggccc acgcgcggtc
     1861 gtagacggcg agcaggtcgt cgagcggcgt gtagttcccg acggcgcggc gccgcaggta
     1921 gtactcgacg accttgtgca cgacggcgcc gtacgcgacg gcatggtgcc gccggatcgg
     1981 gatccgccgc acgtggacgt tgaggtattt cagcggacag gtctgatagt cgtcgatctg
     2041 cttgtggctg acgaccagct cggcgtccgg cgccagcggc tgaagcgcgg aatcgtcggc
     2101 cgcgagcgag ggcgcgaagt gctcgatcgc ctcgacgggg cgcgccttga agggccgcgc
     2161 tgcgtcgcgc ggcaggtcga gcgcctccag cacgaactcg ctcaccttgc gccggcgggt
     2221 gccgccgtag tcggcggcac tcgtgaggta cagctcgtgg cgcgcgcgcg tcatgcccac
     2281 gtagaagagc cggcgctcct cctgcacgtg gacgtccccc gacaccaggg cgtccttgac
     2341 cagtgccacc ggcagctcga gcgcgtcgcg ccgccgccgc gagggaaact tctcgtggac
     2401 gaggttcacg aggaagacca cggggaactc caaccccttc gccttgtgga cggtgagcac
     2461 gcgcacggcg ggcgtctcga ggtccgcctc ggccactgcc gggtcctcgc cggcctcgat
     2521 gagcgcatcg agatgcttga cgaactcccg cacgttgtcg taccggaggg cctgggaggc
     2581 ggacttcacc cggtcgaaga acttcgcgat gttcttggtc tcggcgacgt cccgcgccgt
     2641 ctcctcccgg tacatgcggc cgagccagcc ggagtccttg aggaacagat agagcagctc
     2701 gcccgtcggc gtctcccgcc cgagctccat gtaccgctcg aggtcggcga ccaggtgccc
     2761 gatcgccgcg tgtcccgcct cgtcgatctc gtctcgcagg tcgcggatct cgccggttcg
     2821 cctcagcacg tcgaagaggt ggacgtgctt gcggtcggcg taggtcgagc agcgcgtcag
     2881 gtccgcgatg gggacctggt acaggtccga ggaggcgagg tagtgcaggc tgaccgactc
     2941 gtcgaggtgc acgaccgcac ggaggaaggc gatgagcagt cggatctccg ggcgaccgta
     3001 gaggccggcg ttgcccgaaa acgtccacgg gatgctgcgg aggttcagtg atctcaggaa
     3061 ctgatcggcg tcgtcgttcg accgcacgag gatggcgacg tcgtcgtgct tccacgcgcc
     3121 caccccgacc cgctcgcgga tcatggcggc gacggcctcg gcctcctgcg tgcccgtctc
     3181 gtagtgcagg tgaaccggcg gcgaccccgt caccgcgcgc gcggcgacga ggcgcttgct
     3241 gatgcccctc cggacctcga ggcggtcggg gttgttgtgg acgatgagcc ggtaggccgc
     3301 gtcaaggctc gcctggtgcg agcggtagtt ctccgtgagt accacctgtc gcgcctcggg
     3361 atagcggtcg agaaagccga ggatgttgga cacggccgcg ccacggaacc gatagatggc
     3421 ctggtcctcg tcgccgacgc acgcgacgtt gccgtggcgc gcggcgagca gcttcaccag
     3481 ctcgaactgc gcgtggttcg tgtcctggaa ctcgtccacc atgatgtact tgaaacgccg
     3541 ctggtaggcg ctcagcacgt acgggcgcgc gcggagcagg cggagcacgt ggacgatctg
     3601 gtcgccgaag tcgacggcgc cctcgcgcgc catcagctcc tggcacttcg cgtaggtcgc
     3661 ggccagctcc cgctgctggg ccgcgcgctc gcgcgcctcc tcgtagtcgg gggccgccgg
     3721 cgcctcgcgg gccagccgct cggcgtacgc caggtacgcc tccggcgaga cgtcctcgtc
     3781 cttgcagcgg ctgatgagcg tgatgagggc ctgcacgtgg cgggtggggt cgccgagtgg
     3841 ccggtagtgc tcgaggggca ggtcgaagag ccggtcgcgg acgaagatga cctgctcggc
     3901 gcggttcagc acgcggaagt ccggagtgag gccgatctcc agcgcgtgct cgcgcaggat
     3961 gcggtcgccg aaggcgtgaa acgtcgagat ctcgacgtcc gcgtagccgt acgggacgag
     4021 cgtgtcgacg cgctcctcca tctccgcggc cgccttgtcg gtgaaggtca gcgcgaggat
     4081 ctcctcgggg cgcgcgcggc gctgcgcgat gagccaggcg atgcggcggg tgatgacgct
     4141 cgttttgccg gtgccggcgc cagcgacgat caggagcgga cccacgtcgt gggtgacggc
     4201 ctcccgctgc gcctcgttga gcccctcgag gatgcggtcc accgccatgc taccgggact
     4261 atactacagc cgtcgccaat gcggctcacg atccgcgcca agctggtcct gctcagcctg
     4321 ccgatcctgg tcgtcgtctc gttcggcttc atgctgttcc agcagcgcct gtcgcgaggc
     4381 tgggtcgagg aagacctacg cgagcgcgcg atcgtgttcg cccgcgagat ggccgccacc
     4441 atcggtgacc gccgggagtt cgagagcggc gagctcctgg cgcggcagat cgggcagatc
     4501 atggacgtgc ggcccagcgt gctgcagctc gacgtcctcg ccttcgatgc gacgggcagc
     4561 cgcgtgatgg ccacgagcca cccgctgagc cgcctgcccg tcacgcgccg ggacgccgac
     4621 caggtgcgcc gcgggcgcgc cgtgtcgcga ctcgtcgacg ccgagcgcgc gcggtactgg
     4681 gaggtcgtcg cccccgtcgc cctcgacggc ggcgtggtgg gcgccgtcgc cgcgaagttc
     4741 tcgctggcgc gcgccgacgc cctggcggcg cgcatcggca ccgccgcgct g
//
LOCUS       DATIAZ010000370         6765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_123359, whole genome shotgun sequence.
ACCESSION   DATIAZ010000370 DATIAZ010000000
VERSION     DATIAZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123359"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..596
                     /locus_tag="VLF19_12585"
     CDS             237..596
                     /locus_tag="VLF19_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HSE94137.1"
                     /translation="MRAWRFAPVGIGAVLVAMVAPAAAHHGWSGYDSSQPLTLTGVIR
                     EAGYEHPHGYVRLQLPGKTWLVVLAPPSRMERRGLPASTLAVGTAATVVGYPNRTDAQ
                     EMRAERITVNGKTVELR"
     gene            593..1090
                     /locus_tag="VLF19_12590"
     CDS             593..1090
                     /locus_tag="VLF19_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94138.1"
                     /translation="MSHAPAGPAWLVWLETTALADAMRQWLWLYPIVEMLHIAGIVLL
                     VGAAAMFDFRLLGFSKRLPVSDMARHLLPWARVGLGALGATGVLLFVAHATEFASNPA
                     FQLKVALIAVACLNAAIFHRWPFRAVSAWNVSAEAPGSAKAAAVASLTLWAGVIACGR
                     LLAYL"
     gene            1095..1946
                     /locus_tag="VLF19_12595"
     CDS             1095..1946
                     /locus_tag="VLF19_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSE94139.1"
                     /translation="MNAPLLAAMAAGLTLLLVAERRWPLRRAVDPGPSRRRVNLAVGA
                     ITAVAMQAVLLPAVVGVAAAVEARGWGLLGVLANVVPLPPVLSLLAALALLDYTFYWW
                     HRLLHGVPLLWRFHLVHHTDLDLDVMTAARFHVGEYALGAGFRVLQVLVIGSGPVATG
                     LFEGLVVAAAAFHHANVRLPLALERRLVRLLVTPRMHGIHHSTVERETNSNWSTVLTV
                     WDRLHDTLRLDVAQAAVTIGLPAYRDTRELVLGALLAMPFRRQRPAWAPHHDRPEGGA
                     PRTRLAA"
     gene            complement(1987..3072)
                     /locus_tag="VLF19_12600"
     CDS             complement(1987..3072)
                     /locus_tag="VLF19_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSE94140.1"
                     /translation="MSWTPPPPRPGHPYFMHDAIYAQPGALRLVLRSNADAIAAAAER
                     LRAMDRVLLSGIGTSWHATLVGELLLAQAGGLGHRVRAFHSFEFKNYWPGPDARTGVI
                     VVSHRGTKRFSLEALEKAKAGAGAAVVVTGKGSGEGLGIADVTLRTVEQEASAAHTVS
                     YTCAMALLASVAAEMGGAADLRHELQEIPDHLATLLGQEDWEGLAARFGDRRCYWFVG
                     GGPNTATALEAALKMNEANHTKTVGYECEQFLHGPWAALDAEDVLFVVAPPGPARERC
                     LDAARAAKAVGAPVVALGREDDRELRVLAAETIALPPVPELLSPVVAVVPLQLFTYHT
                     ALRRGVNPDTMRGDQPAHGRARAAISL"
     gene            3516..4403
                     /locus_tag="VLF19_12605"
     CDS             3516..4403
                     /locus_tag="VLF19_12605"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSE94141.1"
                     /translation="MDVPSLQALGALAALGSAATWAVTSLLVRRLMPPYTSVGVGAIR
                     SCVGGALLLGMILLLHGPAPLLAIPSGSLALLVVSIVAAICVGDTAFFESTRRLGLAR
                     GMSISTTYPLVAAVLAATLLGEPLTARVAIGALLTLAGVLLIVVRRHEPGIARDGWWL
                     GVAGAVIASVAWGVSAVALKPALREVDAVTAQAVRLPIAGALLFATPWARGTVSRLRG
                     ERTGLRSLVVLGLLTSVSSVMFVVGLKHAGVAVGSALSATAPMFAIPLGLVFLGERLT
                     AAPLVGAAVTIAGIAVLQT"
     gene            4418..4618
                     /locus_tag="VLF19_12610"
     CDS             4418..4618
                     /locus_tag="VLF19_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94142.1"
                     /translation="MAGTRVANIGSCGRRRRTILGVVSVALGAAVVVAVAATGISPGW
                     ALVAFVPFTFGMLGFVQAGKST"
     gene            4619..4795
                     /locus_tag="VLF19_12615"
     CDS             4619..4795
                     /locus_tag="VLF19_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94143.1"
                     /translation="MRLAARGARETESGTQQVSDPEEAQLLKDQARRVVAEALALGAL
                     LTGLGLFGSAWFQA"
     gene            complement(4975..6402)
                     /locus_tag="VLF19_12620"
     CDS             complement(4975..6402)
                     /locus_tag="VLF19_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE94144.1"
                     /translation="MLDLVVRGGQVVTPHGVGVFDIGIQGEQIVLVGPPGTLPATART
                     LDATGTVVVPGGVDPHTHLAHPIMSHPEEPGITMGPEDDTRGMVFGGTTTHIDFTFVR
                     PGGDIPGAIEPRTARWKSNSYNDYAFHVTLAGPLPLKVFEQIPEAVQEGFASFKVFTT
                     NILPPHPKRAGNRIDFGRIQHAMGKIAAAGGLMTVHGEDEDIVQFMYEKFREEGRMEG
                     WNMHLVHNKLSELLAFRRTVAVARHTGCAVYFVHTSAREGVETIREARAAGLPVYGET
                     LHQYTCFDADHYRTPRGFCSHTYPSLKTPDDRQALWDGLVRDGLSTLATDEYPTTLAL
                     KLKGKTIEDVTGGNVGAEARMGIAYTEGVVKRGMTLERFAAITSTNAARIFGLYPRKG
                     AIAPGSDADLAIVDPRVRKTLARDDFHVTDYSPWEGWEIQGWPVTTLLRGRVVVEQGR
                     LVDDRRLGRLVPRKIDSAVLNRPAC"
     gene            complement(6452..6700)
                     /locus_tag="VLF19_12625"
     CDS             complement(6452..6700)
                     /locus_tag="VLF19_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94145.1"
                     /translation="MKLTAEERRRMFETQQKAREDLLTRLHAPVLAAGPSIEPGHAIR
                     RLLKPAMVVLLLGGGLLAYQTFDFHLPHSLMEALLPRL"
BASE COUNT          814 a         2416 c         2496 g         1039 t
ORIGIN      
        1 gggcgtcgag cgcggcggcg aggccgcgcc gcggcgggcg gagccgccgc tcgcatcgcc
       61 cggcggtggt cccgaccccg cccccaggca tcgcggccgc cggggccggc gaccggaagg
      121 ttcgccgccc gcccagccgt agccgtccgc gcccatcggg cgatcggcgc gcgctcgacg
      181 ccacgcggtg gcccgacgcg tcgcatcggt ctggtaggct gcgcgaggag gttgacatgc
      241 gtgcatggag gttcgctccg gtcgggatcg gcgcggtgct cgtcgcgatg gtggcgccgg
      301 ccgcggcgca ccacgggtgg agcggctacg actccagcca gccgctgacg ctgaccggcg
      361 tcatccggga ggccggttac gaacacccgc acggctacgt gcgcctgcag cttccgggca
      421 agacctggct cgtcgtgctc gccccgccgt cgcgcatgga gcgccgcggc ctgccggcgt
      481 cgacgctggc cgtgggcacc gcggcgaccg tcgtcggcta cccgaaccgc acggacgctc
      541 aggagatgcg cgcggagcgg atcaccgtca acggtaagac cgtcgagctg cggtgagcca
      601 cgcgccggcc ggtccggcct ggctcgtctg gctcgagacc acggcgctcg cggacgccat
      661 gcggcagtgg ctgtggctct acccgatcgt cgagatgctc catatcgccg gcatcgtcct
      721 cctcgtgggc gccgcggcga tgttcgactt ccggctcctc gggttctcga agcgcctgcc
      781 cgtctcggac atggcccggc acctcctgcc gtgggcgcgc gtcgggctcg gcgcgctggg
      841 ggcgaccggg gtgctcttgt tcgttgccca cgcgacggag ttcgcctcca acccggcctt
      901 tcagctgaag gtcgcgctca tcgcggtcgc ctgcctgaac gccgccatct tccatcgctg
      961 gcccttccgc gccgtgagcg cctggaacgt gagcgccgag gcgccgggct ccgcgaaggc
     1021 tgccgccgtg gcctcgctga cgctgtgggc cggggtcatc gcatgcgggc gcctgctggc
     1081 ctacctctga gctcgtgaat gcgcccctgc tcgccgccat ggcggcgggc ctcacgctgc
     1141 tcctggtggc cgagcgtcgc tggccgctcc gccgcgcggt cgaccccggg ccgtcgcggc
     1201 gccgtgtgaa cctcgccgtg ggcgcgatca ccgcggtggc catgcaggcg gtcctgctgc
     1261 cggcggtcgt cggcgtggcc gcggccgtcg aggcgcgcgg ctggggcctg ctcggggtcc
     1321 tggcgaacgt cgttccgctc ccgccggtcc tcagcctcct tgcggcgctc gcgctcctcg
     1381 actacacgtt ctactggtgg catcggctgc tgcacggggt gcccctcctg tggcggttcc
     1441 acctcgtgca ccataccgac ctcgatctcg acgtgatgac cgccgcgcgc ttccacgtcg
     1501 gcgagtatgc gctgggcgcc ggcttccgtg tcctgcaggt cctcgtgatc ggatccggtc
     1561 cggtcgccac cggtctcttc gagggtctcg tcgttgcggc cgccgcgttc caccacgcca
     1621 acgtccggct gccgctcgcg ctcgagcgcc ggctcgtccg gctgctggtc acgccgcgca
     1681 tgcatggcat ccaccactcg accgtggagc gtgagacgaa ctcgaactgg tccaccgtgc
     1741 tcacggtgtg ggaccggctg cacgacaccc tgcggctcga cgtggcgcag gcggccgtga
     1801 cgatcgggct gccggcgtac cgtgacacgc gggagctcgt cctcggggcc ctgctggcga
     1861 tgccgttccg gcgccagcgg ccggcatggg cgccacacca cgaccggccg gagggcggag
     1921 cgccgcggac ccggctggcc gcctgagcgg ccagggactg gaatgcgcct cgcgcgccgt
     1981 cgtcggctag agcgagatcg ccgcgcgagc gcggccgtga gcgggctggt cgccgcgcat
     2041 cgtgtcgggg ttgacgccac gccggagcgc cgtgtggtac gtgaagagct gcagcgggac
     2101 cacggcgacg accggcgaca gcagctcggg caccggcggc agcgcgatcg tctcggcggc
     2161 gagcacgcgc agctcgcggt cgtcctcccg accaagcgcg accaccggcg cgcccaccgc
     2221 cttcgcggcg cgagccgcat ccaggcaacg ctcccgtgcg ggcccgggcg gcgcgacgac
     2281 gaacaggacg tcctccgcgt cgagcgcagc ccagggcccg tgcaggaact gctcgcactc
     2341 gtagccgacg gtcttcgtgt ggttggcctc gttcatcttg agcgcggcct cgagcgcggt
     2401 cgccgtgttg ggaccgccgc ccacgaacca gtagcaccgc cggtccccga agcgcgcggc
     2461 gagcccctcc cagtcctcct gaccgagcag cgtcgcgagg tggtcgggga tctcctgcag
     2521 ctcatggcgc aggtcggccg cgccgcccat ctcggccgcg accgaggcga gcagcgccat
     2581 cgcgcacgtg taggagacgg tgtgggcggc cgaagcctcc tgctcgaccg tgcgcagggt
     2641 gacgtcggcg atgccgagcc cctcgccgct gcccttgccg gtgacgacga ccgccgcgcc
     2701 tgcgccggcc ttggccttct cgagcgcttc cagcgagaag cgcttggtgc cgcggtggct
     2761 caccacgatg acgcccgtgc gcgcgtccgg gccgggccag tagttcttga actcgaacga
     2821 gtggaaggcg cgcacccggt ggccgaggcc tccggcctgc gcgaggagca gctcgcccac
     2881 gagcgtcgcg tgccacgacg tgccgatgcc ggacaggagc acgcggtcca tggcgcgcag
     2941 ccgctcggcc gccgcagcga tcgcgtctgc gttcgagcgc aggaccaggc ggagggcgcc
     3001 cggctgggcg tagatcgcgt cgtgcatgaa gtagggatgt ccggggcgcg gcggcggcgg
     3061 ggtccagctc atggcgcctg gactctaggc acgcgggggc ggggtgtcaa cgtccaatgg
     3121 acgcgcggtg cgccgtccac cggcgctcag ccattgacct ccggttcgcc ctcgtccacc
     3181 caaggattgc gaggcggctg tgtcgcgccc gggtggtagc ggtggccgcg cgggccgtcg
     3241 gccggcgcgg gacgcacctt ccgcggccgc cggcggggtc ccagcgtggg cgtgtcgtgc
     3301 gccggtgttt ccccggcgcc cgtcttcgca cggcgacggc tggccatcgt ctccagaccg
     3361 agcaacgccg gtgccatcgt gtatggtaat gcgatgacaa cctcagggat ctcgcggccg
     3421 cgtgcggcgc ggcgcggccg ccaggagctc ctgctgacgg tcgcgcccca gcggctcgcc
     3481 gcagccatcg gctcccccat cgcgctgttc tgagcgtgga cgttccctcc ctccaggcgc
     3541 tcggggcgct ggcggcgctt ggctcggccg cgacgtgggc cgtcaccagc ctgctggtgc
     3601 gccgcctcat gccgccctac acgtccgttg gcgtcggcgc gatccgctcg tgcgtcgggg
     3661 gggcgctgct gctcgggatg atcctcctcc tgcacggccc cgccccgctg ctcgcgatcc
     3721 catccgggag tctggcactg ctcgtagtgt ccatcgtggc ggcaatctgt gtcggcgaca
     3781 ccgcgttctt cgagtcgacc cgccggctcg ggctggcgcg cgggatgtcg atctcgacga
     3841 cgtacccgct cgtggcggcc gtgctcgcgg cgaccttgct gggcgagccg ctcaccgcgc
     3901 gcgtggccat cggagcgctg ctgacgctgg ccggcgtcct gctcatcgtc gtgcgacggc
     3961 acgagccggg catcgcgcgg gacggctggt ggctcggcgt cgccggagcg gtgatcgcct
     4021 cggtggcctg gggcgtgtcg gcggtcgcgc tcaagccggc gctgcgcgag gtggacgcgg
     4081 tgaccgctca ggccgtgcgg ctgccgatcg ccggtgcgct cctcttcgcc acaccgtggg
     4141 ctcggggcac ggtgagccgg ctcagaggcg agcgcacggg cctgcgcagc ctcgtggtcc
     4201 tcggcctgct gacctcggtg agctcggtca tgttcgtcgt aggcctcaag cacgcgggcg
     4261 tggcggtcgg ctcggcgctg tccgcgaccg cgccgatgtt cgcgatccca ctggggctcg
     4321 tgttcctggg cgagcggctt accgcggcgc cgctcgtcgg cgccgccgtg accatcgccg
     4381 gcatcgcggt gctccagacc tgaaggggag ggcagccatg gcggggacgc gtgtcgcgaa
     4441 catcggctcg tgcggccggc gccgccgaac gatcctgggc gtcgtgagcg tcgccctcgg
     4501 cgccgccgtg gtcgtggcgg tcgcggccac ggggatctcg ccgggctggg cgctggtggc
     4561 gttcgtgccg ttcacgttcg gcatgctggg attcgtgcag gctggcaagt ccacctgagt
     4621 gcggctcgcc gcgcgcggtg cgcgcgagac cgagagcggg acgcagcagg tgtccgatcc
     4681 cgaggaggcg cagctcctga aggaccaggc ccgtcgggtc gtggcggagg cgctcgcgct
     4741 cggcgcgctg ctgaccgggc tgggactttt cggctcggcc tggttccagg cctagagaag
     4801 ccggggtggg taccggtccg ccgacctgtc ccccgcccag cacacttcac ccgacgggac
     4861 gatggaaatg cacaccgcag gacaacgcgc aacgcatcag gggtcgccgg acccgtgccc
     4921 accgcggccc cggcccggcc ggggggacgt cccgcgaact cctgatgcgc gaccctagca
     4981 cgccggacga ttcagcaccg cggagtcgat cttccgcggg acgagccggc cgagccggcg
     5041 gtcgtccacg aggcggccct gctcgacgac gacccgtccg cggaggaggg tcgtcaccgg
     5101 ccagccctgg atctcccagc cctcccacgg cgagtagtcc gtcacgtgga agtcgtccct
     5161 ggcgagcgtc ttccggacgc gcggatcgac gatggcgagg tcggcgtcgc tgccgggggc
     5221 gatggccccc ttgcgcggat agaggccgaa gatcctcgcg gcgttcgtgg aagtgatggc
     5281 cgcgaagcgc tccagcgtca tgccacgctt caccacgccc tcggtgtagg cgatgcccat
     5341 gcgggcctcg gcgccgacgt tgccgcccgt cacgtcctcg atcgtcttgc ccttgagctt
     5401 cagcgcgagc gtcgtcgggt actcgtcggt ggcgagcgtg ctgagcccgt cgcgcacgag
     5461 cccgtcccag agcgcctggc ggtcgtccgg cgtcttgagc gacggatacg tgtgcgagca
     5521 gaagccccgc ggggtccggt agtggtcggc gtcgaagcag gtgtactggt ggagcgtctc
     5581 gccgtacacg ggcaagcccg cggcgcgcgc ctcgcggatg gtctcgacgc cctcgcgcgc
     5641 ggaggtgtgg acgaagtaaa cggcgcagcc cgtgtggcgc gcgacggcga cggtgcggcg
     5701 gaaggcgagc aactcggaga gcttgttgtg gacgaggtgc atgttccacc cctccatccg
     5761 gccctcctca cggaacttct cgtacatgaa ctgcacgatg tcctcgtcct caccgtgcac
     5821 ggtcatgagt ccgccggcgg cggcgatttt gcccatggcg tgctggatgc ggccgaagtc
     5881 gatccggttg ccggcgcgct tgggatgggg tggcaggatg ttggtcgtga acaccttgaa
     5941 cgaggcgaac ccctcctgca ccgcctcggg gatctgctcg aacaccttga gcggcagcgg
     6001 tccggcgagc gtgacgtgaa aagcgtagtc gttgtacgag ttgctcttcc agcgtgccgt
     6061 ccgcggctcg atcgcgccgg ggatgtcgcc gccgggccgg acgaacgtga agtcgatgtg
     6121 ggtcgtggtg ccgccgaaca ccatcccacg ggtgtcgtcc tcggggccca tcgtgatccc
     6181 gggctcctcg ggatggctca tgatcgggtg cgccaggtgc gtgtggggat cgacgccgcc
     6241 cgggacgacg acggtgccgg tcgcgtccag cgtgcgcgcg gtcgcgggta gcgtgccggg
     6301 cggaccgacg aggacgatct gctctccctg gatgccgatg tcgaaaacgc cgacaccgtg
     6361 aggggtgacg acctgaccgc cgcggacgac gagatcgagc atgccgcgga ttatacgacc
     6421 gatgcggggc cggtgtcggc ccgcgccggc cctacagacg cggcagcagg gcctccatca
     6481 aggagtgtgg caggtggaag tcgaacgtct gataagcgag caggccgccg cccagcagca
     6541 ggacgaccat cgccggcttg aggagacgcc tgatcgcgtg accgggctcg atgctcgggc
     6601 cggccgcgag aaccggcgcg tgaagccggg tgagcaagtc ctctcgcgcc ttctgctgcg
     6661 tctcgaacat ccgccgccgc tcttccgcag tgagcttcat ctgtccctcc gagctcacga
     6721 gtgcttctgg tgccccgatg ctgatgcaaa gatctcgccg gctcc
//
LOCUS       DATIAZ010000371        16142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1175056, whole genome shotgun sequence.
ACCESSION   DATIAZ010000371 DATIAZ010000000
VERSION     DATIAZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 16142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16142
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175056"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VLF19_12630"
     CDS             complement(<1..415)
                     /locus_tag="VLF19_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSE94146.1"
                     /translation="MPVKLFVGGLSFSTTNESLHAAFARFGTVTSAIVMTDRATGRSR
                     GFGFVEMGTDQEAERAISSLNGAQLDGRMIRVDKATPRGTAPARRPGGPGGGFAPRGP
                     GGGGFAPRGPGTGGGFAPRGPGGGFAPRAPGPGGGF"
     gene            complement(455..2173)
                     /gene="polX"
                     /locus_tag="VLF19_12635"
     CDS             complement(455..2173)
                     /gene="polX"
                     /locus_tag="VLF19_12635"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HSE94147.1"
                     /translation="MRNFVVARLFYEVATLLEVNDESRFRVRAWQRAAQTLETLGEDV
                     AAVAARGGLTALPGIGRELAARIEEYLATGRIAVLETLRGGLPPRFLTLLEIRGLGPR
                     TARLLWERLGVDSVERLEEVCRDGSVLTVPGVQRKTCENVLKGIALWRAGRARTPLSR
                     AREVAARVVETLRAHGGVERIEVAGSLRRLRETVKDVDVLVTSTEPTRVIATLSEMPS
                     VVEVIARGDTKASVRHQDGLQIDLRVVEPGAFGAALQYFTGSKDHNVRLRELAKRRGL
                     TVSEYGVFEERTGARVAGATEGDVYAAVGLPWIPPELRENGGEIEAAREGRLPELVEA
                     RAIRGDLHAHTDWSDGHHPVERLVEAAQTRGYEYVIVSDHSRSTAIAGGLTVERLREQ
                     IARIHELQPRYDVRILTGTECDILADGSLDFPDDVLAGLDVVLGAVHSRFKQTRQEMT
                     ARIIRALENPHVDILAHPTGRLLGSRDPYDVDLDAVFAAARRHGKAIEINASPDRLDL
                     ADLHARRAAELGVPIAINTDAHYLSELDNLELGLGVARRAWLGPAQVLNARPLSDLLA
                     WADRDR"
     gene            2199..2564
                     /locus_tag="VLF19_12640"
     CDS             2199..2564
                     /locus_tag="VLF19_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94148.1"
                     /translation="MIAAWCWELVEPYLRRNLLNRGVERPTRRQVLEEFVRVWPEFTA
                     TIGVQEPFAGTIRFKWLARLLSAEMEPFLADPEGWLAGRWGGGKYKMNLHHGLHFVNT
                     RNFKPGGAPRWQAAPALEE"
     gene            2572..2754
                     /locus_tag="VLF19_12645"
     CDS             2572..2754
                     /locus_tag="VLF19_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94149.1"
                     /translation="MASDETRRLLKLFGVAVTNLEEAIDGKAPVEKVAELDEELATRW
                     REVESYLERLRSRRIA"
     gene            2751..3554
                     /locus_tag="VLF19_12650"
     CDS             2751..3554
                     /locus_tag="VLF19_12650"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HSE94150.1"
                     /translation="MTALVDASGATVALSRPPRRIVSLIPSTTETLCALGLAEALAGV
                     TVYCREPADVVRGKTRIGGEKDPDLAKIRALAPDLVIANVEENLREHVDALRAWGIPV
                     WVTYPRTVADGIRMIRELGAVTGADARAAAIAGELEALYGEVTAATARRAPVPVFYAI
                     WREPWMTIGSDTYIHDMLRVCGGANVFADSADRYPTVALDDVAGRRPAVVLLPDEPYR
                     FRRAHLGDFAPYADVPAVRDGRIHLVDGKPFSWHGPRIADALRTLPALF"
     gene            complement(3568..3720)
                     /locus_tag="VLF19_12655"
     CDS             complement(3568..3720)
                     /locus_tag="VLF19_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94151.1"
                     /translation="MDVVLDLICAVISLTFLTILGIVSGVAIPGLFLAAAAPWLDWIL
                     PARKKR"
     gene            complement(3699..4925)
                     /locus_tag="VLF19_12660"
     CDS             complement(3699..4925)
                     /locus_tag="VLF19_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HSE94152.1"
                     /translation="MVLGVVFYSYTYQWFQPGHRGSRPLRMAVNGVLFGLLALALMTW
                     RIELASGDYIDARVVPIALVGLLEGWPAALLAAVLPVLYRVWLGGSGAPAGVTAILAS
                     AALGALAHLWARRDGRLTLRHTLALTAAVYLVTWVSFVLAGAYAVSLFARSWFWLLVT
                     YVVGIVFTGRLMGDVVEQARLMAERGRFRAILDEASEAIRIVDPDTHRIIDVNRRDCE
                     LSGYARAELVGRDDRDRWPAEPELRALHEAAEAEVRQAGACRTFGLPCRRGDGTLVTI
                     DSSRRVVTHGGRRYEIVVGRDAAEREATEAARREAAELRAVTMLAGAAAHEINNPLAV
                     VMGALDLLARDLPGDGRPRRLIDQGLAGVRRVSDIVLRMRTITRVQAQEVAPNLPPIL
                     DIEKSSREEPWTSSST"
     gene            complement(4972..5436)
                     /locus_tag="VLF19_12665"
     CDS             complement(4972..5436)
                     /locus_tag="VLF19_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSE94153.1"
                     /translation="MGAEARLKEKNITLPTPATPVANYVGAVRVGNLLFVSGHGPIRS
                     DGKPMARGKLGRDLSVEQGYHVAREVGLNLLATTRATLGSLDKVRRVVKVLGMVNSAD
                     GFGDQPKVINGFSDLMVDIFGDAIGKHARSAVGMAELPMGIPVEIEMILEVE"
     gene            5498..6289
                     /locus_tag="VLF19_12670"
     CDS             5498..6289
                     /locus_tag="VLF19_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSE94154.1"
                     /translation="MPVKPSPAVPRPAATLVLLRDRPGGGCETLLIRRHEKSRFAAGD
                     FVFPGGRIEPEDAPADVARWCRGLDDATAAGRLGLDAPAALAYWIAAIREAFEEVGVL
                     LACDTAGRPASLDPARLAERRRACQRDHRAFWTLLREENLVLPVDRLVYFAHWVTPEE
                     RPLRFDTRFFAAQAPAGQDAEPDAHEITEVRWLTPAAALAARARGEISLRLPTATNLA
                     LFDGAPAAAAALERLAGREVAMIRPRLVADGAAQRTLLPGQAGWY"
     gene            complement(6311..7573)
                     /gene="pfp"
                     /locus_tag="VLF19_12675"
     CDS             complement(6311..7573)
                     /gene="pfp"
                     /locus_tag="VLF19_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HSE94155.1"
                     /translation="MRRRDARMGGIGKTLAILVGGGPAPGINAVIAAATIEARNHGLR
                     VLGCYDGFRWLVQGDTQHVAELSIDDVSRMHFEGGSILRTSRTNPTRTPDGVARVADT
                     LQRLGVHHLITVGGDDTAYASSRLAQALPGLTVAHVPKTIDNDLPLPSDIVTFGFTTA
                     VNLGKELVRNLMQDAATTERWFFVTVMGRHAGHLALGIGGSAAATLSLIAEEFPAPHV
                     AVDRLVDTLEGAIIKRRVHGREHGVAILSEGLAEKLDPETLGPVERDQYGNVRLAELE
                     LGRMLKERVQASLKSRGIDVTIVDKEMGYELRCAPPGAHDIQYCRSLGYWATRFLLEG
                     GSNAMVTIQAGRLVPVPFGLMMDPKTGKIRVRYVDVESEMYQTLWAYMIRLKPEDFDD
                     EQMLAALAKAGNMSEPEFLTRFGPLVGR"
     gene            7791..8315
                     /locus_tag="VLF19_12680"
     CDS             7791..8315
                     /locus_tag="VLF19_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94156.1"
                     /translation="MTAQTGASTVVAPTASHDPARLYQEGIIAGVIGAATVAAWFLVL
                     DALSGRPLWTPTVLGSALFRRGLPPGPLEALPVSLEMVLMFTWVHGLVFAAIGGLASR
                     LLGLAERNPSVGFGVLLLFVVFEAGFTVVAMVLAAPVLQALTWPAVLVANLLAAAAMG
                     VYFWFRHPALTVSP"
     gene            complement(8497..9738)
                     /gene="ccrA"
                     /locus_tag="VLF19_12685"
     CDS             complement(8497..9738)
                     /gene="ccrA"
                     /locus_tag="VLF19_12685"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HSE94157.1"
                     /translation="MKDLYDLGDRPPLGEVPAKMHAYVVRQSRFGQPRDAWKREVIST
                     PALGADEVLIYVMASGINYNNVWAALGYPLDVIAERQKTGEPEDFHAGGSDCSGIVWA
                     VGRDVDTVAVGDEVIVHSGWWRRDDPWVRAGKDPMLAESTRIWGYQTNYGSYCQFARA
                     QGHQCLPKPRRLTWEEAGCFLLCGSTAYRMLMGWPPHTVEPGDIVLVWGAAGGLGSMA
                     LEITRALGGRPIAVVSDEDKRKFCVDHGAVGVVNRTGFTHWGKMPDTTDARAYGEWAK
                     GARAFGKAVWDALGERVGPKIVFEHPGEATLPTSEFVCATGGMIVICAGTTGYNVTLD
                     LRYHWMRQKRFQGSHLSNDPQAAAVTALVAEGKVDPCLSRTFGFDDIPECHQLMLDNR
                     HPYGNMGVLVNARQPGTGRTA"
     gene            complement(9752..10771)
                     /locus_tag="VLF19_12690"
     CDS             complement(9752..10771)
                     /locus_tag="VLF19_12690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HSE94158.1"
                     /translation="MKLDVGMPTHDLKSIPAYARKVEALGFDCLWSSETQHDPYLPLA
                     VAATVTARMKLGTAIAVVFPRSPMITAHIAWDLQKASDGRFILGLGTQVKGHNERRFS
                     VKFESPGPKMREAVRALRAIWDCWQNGTKLNFKGEFYRFDLMTPFFSPGPIDHPKIPI
                     YIAGVNRYICRVAGEVCDGLHVHPFNSAKYLREYVHPAVEEGLRASGRTREDFTYSTA
                     SFVVIGDTEEERARARQAVRQQIAFYASTRTYEPVLAAHGWQDLVPHLHRKSVEGDWV
                     SMASLVTDEMVETYAVTGTYDTIAAALQERYAGLLDRTAFYEPQHRGGLDDPRLARVV
                     KQFNG"
     gene            complement(11127..11975)
                     /locus_tag="VLF19_12695"
     CDS             complement(11127..11975)
                     /locus_tag="VLF19_12695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSE94159.1"
                     /translation="MRALVLAVLVLAAPVWAADYAGPIIDAHSHVSSSKAIDAYAGAA
                     KRHNVKKVVLLGVGGAQRDDDAWITGAAKKYPDLVVAAVPVPDPMSATGARMLDEALV
                     ARRGRVAGEVHVRQVGSRNIDRDPASPAFGRVLEIVAKHGVPLVIHYELTDQATAALD
                     RALAAHPKATVVLAHAGEGPPARVQGLLERNANLMVDLSGMHFQRKPALATETGPLDR
                     DWKTLIEKMPDRFLIGIDVWSPRLLEPVMLDRLMKWTRRILGELPPDAAESVAHRNAA
                     RLFRIE"
     gene            complement(11976..12098)
                     /locus_tag="VLF19_12700"
     CDS             complement(11976..12098)
                     /locus_tag="VLF19_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94160.1"
                     /translation="MVDLVRVVGISHVPTEVTQEMWINSTGLVRTMLPVPKEAK"
     gene            complement(12134..12286)
                     /locus_tag="VLF19_12705"
     CDS             complement(12134..12286)
                     /locus_tag="VLF19_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94161.1"
                     /translation="MQFEVEVYRNEAGDWIAEAVEYKVTAKGLTEKEALARVMDALAQ
                     HFKNLK"
     gene            complement(12423..13508)
                     /locus_tag="VLF19_12710"
     CDS             complement(12423..13508)
                     /locus_tag="VLF19_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HSE94162.1"
                     /translation="MTAWRTDVVGSLLRPAALVKARADREAGQLDAAAFKRVEDRAVD
                     EAIALQEQVGLDVVTDGELRRYAFFGHLVEALDGFDRFGGWAIPFHNEQGEELVFKRP
                     VVVERLRWRRSMCAEEFVYGRARARRPVKVTLISAQQAAAYWDPGKSAAAYPTRDAYL
                     ADVVDFTRREVEELARLGCTYVQIDAPQYAALLDPKIRAGYVERGADPDTMLDACVDM
                     DNAVIAGHPGITFGLHICRGNNQSMFYASGGYDPIERVFRRTRFQRFLLEYDDERSGG
                     FGPLRAVPDDRVVVLGLVSTKKPALEPLETLKSRITEAAASVPLERLALSPQCGFAST
                     MEGNRVTEDDQRKKLERVVAAAREVWG"
     gene            complement(13520..13987)
                     /locus_tag="VLF19_12715"
     CDS             complement(13520..13987)
                     /locus_tag="VLF19_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSE94163.1"
                     /translation="MEKHHVSWSDVERMVGKLLRTLSRDYDNILVVTRGGMVPACLIS
                     ERTGIRNILCAAVVLYDGEERALPAPAFVQFPDDRHVEGRRILVVDDVWDSGSTVVAV
                     RDRLRKAGAAVDVCVLHYKPHHSKFPGDGPDHHAEETDGWIVYPWDPVNPLPQ"
     gene            complement(14043..14471)
                     /locus_tag="VLF19_12720"
     CDS             complement(14043..14471)
                     /locus_tag="VLF19_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HSE94164.1"
                     /translation="MIADVPGWALDLLREARVARLGTADASGRPLVVPVCYAFDGARL
                     FSVIDAKPKRTRDLRRLRNIAENPRVSLVVDEYDEDWTRLRWVIVEGRADVLTDGVEY
                     TGAIDLLMAKYPQYRAMALDRAHGTLIRVIPERLLRWRFA"
     gene            complement(14468..15304)
                     /locus_tag="VLF19_12725"
     CDS             complement(14468..15304)
                     /locus_tag="VLF19_12725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSE94165.1"
                     /translation="MNIGVCMFNTDYAIRIEDLARACEERGFESLWVPEHTHIPVSRK
                     TPFPSGGELPREYKHTLDPFVALAAAAMVTRNLKLGTGILLLIQRDTITTAKEVASLD
                     FVSNGRFLLGVGGGWNQDEMENHGTDFKTRFKRLGEQVRAMKAIWTEETAEFHGEHVN
                     FDPIWMYPKPVQKPHPPVLFGGETEHTLQRVVEIGDGWFPRARSAERIFAGLEDLKTR
                     AARAGRDMKTISVSVFGAPGDAATLDRYRQAGLTRAIIRLPSEPREKVLPLLDQHAKL
                     LR"
     gene            complement(15471..>16142)
                     /locus_tag="VLF19_12730"
     CDS             complement(15471..>16142)
                     /locus_tag="VLF19_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006089643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSE94166.1"
                     /translation="APFGWTNIRPGRGLLPEDDFPKPYLAIWDLSDGLLARLGARIAV
                     PIDPFPGVIGVALEEPGGHSTMPPRKNGGNMDVKQLTAGTTVYLPVWVDGALFSVGDG
                     HGAQGDGEVCITAIEMMAQVTARFDLQPGRRLAEPRFRTRGPVGARTNTGPHFATTAH
                     GHDLYRSAQQAIRYMIDHLVEERGLSREEAYILCSVAVDLRISEIVDAPNWIVSAFLP
                     ESIFV"
BASE COUNT         2218 a         5721 c         5787 g         2416 t
ORIGIN      
        1 cgaaaccgcc gccggggccc ggtgcgcgtg gcgcgaagcc gccaccagga ccgcgcgggg
       61 cgaagccgcc gccagtcccc gggccgcgcg gcgcgaagcc acctccaccc gggccgcgcg
      121 gggcgaaacc gccgccgggc ccgccgggtc ggcgcgccgg cgccgtgccc cgcggcgtcg
      181 ccttgtccac ccggatcatc cgtccgtcga gctgcgcgcc gttcaggctg ctgatggcgc
      241 gctcggcctc ctggtcagtg cccatctcga cgaagccgaa gccgcgcgag cggccggtcg
      301 cacggtcggt catgacgatg gcggacgtca ccgtgccgaa gcgggcgaac gccgcgtgaa
      361 ggctctcgtt cgtggtgctg aacgagagac ctccgacgaa cagcttcacg ggcatgacgg
      421 aactcctttc gtgtcggcgc gacgcggcac gcgatcagcg gtcacggtcg gcccaggcga
      481 gcaggtctga caggggccgc gcgttcagca cctgcgcggg gccgagccag gcgcggcggg
      541 cgacgccgag gcccagctcg aggttgtcga gctccgagag atagtgcgcg tcggtgttga
      601 tggcgatcgg cacgcccagc tcggcggcgc gccgggcgtg caggtcggcg aggtcgaggc
      661 ggtccggaga ggcgttgatc tcgatggcct tgccgtggcg gcgagcggcg gcgaagacgg
      721 cgtcgaggtc cacgtcgtac gggtcgcgcg atccgaggag ccgtcccgtc gggtgcgcca
      781 ggatgtccac gtggggattc tcgagcgcgc ggatgatgcg cgcggtcatc tcctggcgcg
      841 tctgcttgaa gcgcgagtgg acggcgccga gcacgacgtc gagcccggcc aggacgtcgt
      901 ccgggaagtc gagactgccg tcggcgagga tgtcgcactc ggtcccggtg aggatgcgga
      961 cgtcgtagcg cggctgcagc tcgtggatgc gcgcgatctg ctcgcgcagc cgctccacgg
     1021 taagccctcc ggcgatggcc gtggaccgcg agtgatccga cacgatcacg tattcgtacc
     1081 cgcgcgtctg agcggcctcc acgaggcgct cgaccgggtg gtggccgtcg gaccagtcgg
     1141 tgtgggcgtg caggtcgccg cggatggccc tggcctcgac gagctcgggc agccggccct
     1201 cgcgcgcggc ctcgatctcg ccgccgttct cccgcagctc gggcgggatc cagggcaggc
     1261 ccacggctgc gtagacgtcc ccctcggtgg cgccggccac gcgcgcgcca gtgcgctcct
     1321 cgaacacgcc gtactcgctg acggtgaggc cgcgacgctt ggccagctcg cgcagccgga
     1381 cgttgtggtc cttcgaaccc gtgaagtact ggagcgcggc gccgaaggcc ccgggctcca
     1441 cgacgcgcag gtcgatctgc aggccgtcct ggtggcggac cgacgccttc gtgtcgccgc
     1501 gcgcgatgac ctcgacgacc gacggcatct cgctcagcgt ggcgatgacg cgggtgggct
     1561 cggtggaggt gaccagcacg tcgacgtcct tgaccgtctc ccgcagccgc cggagcgagc
     1621 ccgcgacctc gatgcgctcg acgcccccgt gggcccgcag cgtctcgacg acgcgcgcgg
     1681 cgacctcgcg ggcccgggag agcggcgtcc gggcgcggcc ggcgcgccac aacgcgatgc
     1741 ccttgaggac gttctcgcac gtctttcgct ggaccccggg gacggtgagg accgagccgt
     1801 cccggcagac ctcttccagg cgctcgaccg agtccacgcc cagccgctcc cagagcagcc
     1861 gcgccgtgcg gggcccgagc ccgcgaattt cgagcagcgt gaggaagcgc ggaggcaggc
     1921 cgccgcgcag ggtctcgagc acggcgatgc ggccggtggc gaggtactcc tcgatgcgcg
     1981 ccgcgagctc gcggccgatg ccgggcagcg cggtcagtcc cccgcgcgcg gcgaccgcgg
     2041 cgacgtcctc gccgagcgtc tcgagcgtct gggccgcccg ctgccaggcg cgaacccgga
     2101 aacggctctc gtcgttcacc tccaggagcg tcgcgacctc gtagaagagg cgcgccacga
     2161 cgaagtttcg cacggccaca tgatacactc ggcccgtcgt gattgccgcc tggtgctggg
     2221 agctggtgga gccctatctc cggcgcaatc tcctgaaccg tggcgtcgag cggcccacgc
     2281 gccggcaggt cctcgaggag ttcgtccgcg tctggcccga gttcaccgcc accatcggcg
     2341 tccaggagcc cttcgctggc accatccgct tcaagtggct cgcgcggctg ctgtcagccg
     2401 agatggagcc gttcctggcc gaccccgagg gctggctcgc cggccggtgg ggtggcggaa
     2461 agtacaagat gaacctgcac cacgggctgc acttcgtgaa cacgcgcaac ttcaagcccg
     2521 ggggcgcgcc gcgctggcag gcggcgccgg ccctcgagga gtgagcgagc gatggccagc
     2581 gacgagacgc gcaggctgct gaagctgttc ggggtcgcgg tgacgaatct cgaggaggcg
     2641 atcgacggga aggcgcccgt ggagaaggtc gccgagctgg acgaagagct ggccacccgg
     2701 tggcgcgagg tggaatcgta cctcgagcgc ctccgcagcc gccgcatcgc gtgacggcgc
     2761 tggtcgacgc ctccggcgcg actgtcgcgc tctcccggcc gccgcgccgc atcgtctccc
     2821 tgatcccgag caccacggaa actctgtgcg ctctgggtct cgccgaggcg ctcgccggcg
     2881 tgaccgtcta ctgccgcgag ccggccgacg tcgtgcgcgg caagaccagg atcggcggtg
     2941 agaaggaccc cgacctcgcg aagatccgcg cgctggcgcc cgacctcgtg atcgccaacg
     3001 tcgaggagaa cctgcgcgag cacgtcgacg ctctgcgggc gtggggcatc cccgtgtggg
     3061 tcacctatcc gcggaccgtc gccgacggca tccgcatgat ccgcgagctc ggcgccgtca
     3121 ccggagccga cgcgcgggcg gcggccatcg ccggcgagct ggaggccctc tacggcgagg
     3181 tgaccgcggc gacggcgcgt cgggcgccgg tgcccgtgtt ctacgcgatc tggcgcgagc
     3241 cctggatgac gatcgggagc gacacgtaca tccacgacat gctgcgcgtc tgcggcggtg
     3301 ccaacgtgtt cgccgacagc gccgaccgct acccgacggt cgccctcgac gacgtcgccg
     3361 ggcgccggcc ggcagtggtc ctcctgcccg acgagcccta ccgcttccgg cgcgcgcacc
     3421 tcggcgactt cgccccgtac gccgacgtgc ccgcggtgcg cgacggccgg atccacctcg
     3481 tggatggcaa gccgttctcg tggcacggcc cgcggatcgc cgacgcgctc cgcacgctgc
     3541 cggcgctgtt ttgatcaaac gagcccgcta cctcttcttc ctcgccggca ggatccagtc
     3601 gagccacggc gcggccgcgg ccaggaacag accggggatg gccacgcccg acacgatccc
     3661 caggatcgtg aggaaggtga gagagatgac cgcgcagatc aggtcgagga cgacgtccat
     3721 ggctcctccc ggctggactt ctcgatgtcg aggatcggcg gcaggttcgg cgcgacctcc
     3781 tgcgcttgca cgcgcgtgat cgtgcgcatg cgcagcacga tgtcgctcac ccggcgcacg
     3841 ccggccaggc cctggtcgat gagcctgcgg gggcggccgt cgccgggcag gtcgcgcgcg
     3901 agcaggtcga gggcgcccat gacgacggcg aggggattgt tgatctcgtg cgcggcggcg
     3961 ccggcgagca tggtcaccgc ccgcagctcg gccgcctcgc gccgcgcggc ctcggtggcc
     4021 tcgcgctcgg ccgcgtcccg gcccaccacg atctcgtagc gccggccgcc gtgcgtcacc
     4081 acacggcggc tggagtcgat ggtgacgagg gtgccgtcgc ccctccggca gggcaggccg
     4141 aacgtccggc aggcccccgc ttgccgcacc tcggcctcgg ccgcctcgtg cagcgcgcgc
     4201 agctccggct cggccggcca ccggtcgcgg tcgtcgcgcc cgaccagctc cgcgcgcgcg
     4261 tagcccgaca gctcgcagtc gcgccggttc acgtcgatga tgcggtgggt gtcgggatcg
     4321 acgatgcgga tcgcctcgga cgcctcgtcg aggatcgcgc ggaaccttcc gcgctcggcc
     4381 atgagtcgcg cctgctccac gacgtcgccc atgaggcgtc cggtgaagac gatgccgacg
     4441 acgtaggtga cgagcagcca gaaccacgat cgcgcgaaca gcgacacagc gtacgctccc
     4501 gccagcacga aggagaccca ggtcaccagg tagacggcgg cggtcaacgc gagcgtgtgg
     4561 cgcagggtca ggcggccgtc tcgccgggcc cagaggtggg cgagggcgcc gagggcggcg
     4621 gaggccagga tggcggtgac cccggccggc gcgccggagc cgccgagcca cacccgatag
     4681 aggaccggca acacggcggc gaggagcgcg gccggccagc cttcgagcag gccgacgagc
     4741 gcgatcggga cgacgcgggc gtcgatgtag tcgccgctcg ccagctcgat gcgccacgtc
     4801 atgagcgcca gcgcgagcag gccgaacagc acaccgttga cggccatgcg cagtggccgg
     4861 ctgccgcggt gtcccggctg gaaccactgg tacgtgtacg agtagaagac cacgccgagc
     4921 accacgacgc cggccgactc gaggacgtga tgcaggtcga tcgccggtcc tctattccac
     4981 ctcgaggatc atctcgatct cgacagggat gcccatgggc agctcggcca tgcccaccgc
     5041 ggagcgggcg tgcttgccga tggcgtcgcc gaagatgtcg accatgagat ccgagaagcc
     5101 gttgatcacc ttgggctggt cgccgaagcc gtccgcggag ttcaccatgc cgagaacctt
     5161 cacgacgcgc cggaccttgt ccaggctgcc cagggtcgcg cgcgtcgtgg cgagcaggtt
     5221 cagacccacc tcccgcgcca cgtgatagcc ctgctcgacc gacaggtcgc gtccgagctt
     5281 gccccgcgcc atgggtttgc cgtcgctcct gatcggtccg tggcccgaga cgaacagcag
     5341 atttccgacg cggacggcgc cgacgtagtt ggccaccggc gtcgccggcg ttggcagtgt
     5401 gatgttcttc tccttgaggc gggcttcagc gcccatcgga acggatcctc cttcgacgga
     5461 tgtgacagag ccgcggtcat cgtgtcacac tggccgggtg cccgtcaaac cttcgcccgc
     5521 cgtccccagg cctgccgcca cgctcgtgct gctccgcgat cggccgggcg gtggctgcga
     5581 gacgctgctg atccggcgtc acgagaagag ccgcttcgcc gcgggtgact tcgtgttccc
     5641 gggcggccgt atcgaaccgg aagacgcgcc ggccgacgtc gcgcgctggt gccgcgggct
     5701 cgacgacgcg accgccgcgg gccgcctggg cctcgacgcc ccggccgcac tcgcctactg
     5761 gatcgccgcc atccgcgagg ccttcgagga ggtgggcgtg ctgctcgcct gcgacacggc
     5821 cggtcggccc gcgagcctcg atccggcgcg cctggccgag cgccggcgcg cctgccagcg
     5881 cgaccatcgc gccttctgga cgctgctccg cgaggagaac ctcgtgctcc cggtggatcg
     5941 cctcgtgtac ttcgcacact gggtcacgcc ggaggagcgg ccgctccgct tcgacacgcg
     6001 cttcttcgcc gcgcaggcgc cggccggcca ggacgccgag ccggatgcgc acgagatcac
     6061 cgaggtccgc tggctcacgc ccgccgcggc gctcgccgcc cgcgcccgcg gcgagatctc
     6121 gctgcgcctg cccaccgcca cgaacctggc gctcttcgac ggcgcgcccg ccgccgccgc
     6181 ggccctcgag cgtctggccg gccgtgaggt cgccatgatc cggccacgcc tggtcgccga
     6241 cggcgccgcc cagcgcacgc tcctccccgg ccaggccggc tggtactgat ggggcctcga
     6301 acggcgctga ctaacggccg acgagggggc cgaagcgcgt gagaaattcc ggctcgctca
     6361 tgttgccggc cttcgccagc gccgccagca tctgctcgtc gtcgaagtcc tcgggcttga
     6421 gccggatcat gtacgcccag agcgtctgat acatctccga ctcgacgtcg acataccgga
     6481 cgcggatctt gccggtcttg gggtccatca tgaggccgaa ggggacgggc acgagtcggc
     6541 cggcctggat cgtcaccatg gcgttgctgc cgccctcgag caggaagcgc gtcgcccagt
     6601 agccgaggct ccggcagtac tggatgtcgt gggctccggg cggcgcgcag cgcagctcgt
     6661 agcccatctc cttgtccacg atcgtcacgt cgatgccgcg tgacttgaga ctggcctgca
     6721 cgcgctcctt gagcatccgc cccagctcca gctcggccag ccgcacgttg ccgtactggt
     6781 cgcgctccac cgggcccagc gtctccggat cgagcttctc ggcgaggccc tcggagagga
     6841 tcgccacgcc gtgctcgcgg ccgtgcacgc ggcgcttgat gatcgcgccc tccagcgtgt
     6901 ccacgaggcg atcgacggcg acgtgcggcg ccgggaactc ctcggcgatc agcgacagcg
     6961 tggctgccgc cgagccgccg atgcccagcg cgaggtggcc cgcgtgccgg cccatgacgg
     7021 tgacgaagaa ccagcgctcg gtcgtggccg cgtcctgcat gaggttgcgg acgagctcct
     7081 tgccgagatt gacggccgtg gtgaagccga acgtgacgat gtcgctcggc agcggcaggt
     7141 cgttgtcgat cgtcttgggc acgtgggcga cggtgagtcc cggcagggcc tgggccagtc
     7201 gtgacgacgc gtaggcggtg tcgtcgccgc cgaccgtgat caggtggtgg acgcccaggc
     7261 gctggagcgt gtcggccacg cgcgcgacgc cgtccggcgt gcgcgtggga ttcgtgcgcg
     7321 aggtgcgcaa gatggagccg ccctcgaagt gcatgcgcga gacgtcgtcg atgctgagct
     7381 cggccacgtg ctgcgtgtcg ccctggacga gccagcggaa gccgtcgtag cagccgagga
     7441 cgcgcaggcc gtggttgcgc gcctcgatcg tcgccgccgc gatgaccgcg ttgatgccgg
     7501 gcgcgggccc gccaccgacc aggatcgcga gtgtcttgcc gatgccgccc atgcgtgcgt
     7561 ctctccgtct catcctaagc gatcggtcaa cgaaaccgat cgattcacgt gagccccccg
     7621 gcgctcctgc gcgtccggcc gccgcccctt gcgtccgggc cgtcaccggt acgccacccg
     7681 tcgctgccgc cgcggccggc gcccgtgccc gtttgctccc ggcggggccg gggagtatgc
     7741 tgccgccaac cgtcgaagat cggtccacgc acccggaaag gagaggggtg atgaccgctc
     7801 agaccggcgc gagcaccgtc gtcgctccca ccgccagtca cgatcccgcg cggctctacc
     7861 aggagggcat catcgccggc gtcatcggcg ccgcgaccgt cgccgcctgg ttcctcgtcc
     7921 tggacgcgct ctccggtcgc ccgctctgga ccccgaccgt gctgggcagc gcgctcttcc
     7981 gccgcggcct gccaccgggc ccactcgagg cgctgcccgt gtcgctcgag atggtgctga
     8041 tgttcacgtg ggtccacggg ctcgtcttcg ccgcgatcgg aggcctcgcc tcgcggctgc
     8101 tgggtctggc cgagcgcaac ccgagcgtcg ggttcggcgt cctgctgctg ttcgtcgtgt
     8161 tcgaggcggg cttcaccgtc gtggccatgg tcctggcggc gccggtgctg caggcgctga
     8221 cgtggccggc cgtgctggtg gccaatttgc tcgccgcggc ggcaatgggc gtgtatttct
     8281 ggttccgcca cccggcgctg accgtgagcc cgtagccatc ggccgtcgcg gcggcggccg
     8341 ggcgacggcc acgccgtcgg cgcgaagaat ccgtgacgcg ggcacagcgg cggtccgacg
     8401 ccccgagtgc gcgcgtcgcc cgcgcgccac ctcgtccccg atcgtccgca gcaatggcct
     8461 tcccgcacga agagatcgcg ggtggtcggg cggccttcac gcggtgcggc cggtgccggg
     8521 ctgacgggcg ttcacaagga cgcccatgtt gccgtacggg tggcggttgt cgagcatgag
     8581 ctgatggcac tccgggatgt cgtcgaagcc gaaggtgcgg ctgagacagg gatccacctt
     8641 gccctcggcg acgagggccg tcaccgccgc cgcctgcgga tcgttggaga ggtggctgcc
     8701 ctggaaccgc ttctgccgca tccagtggta gcgcaggtcc agcgtcacgt tgtagccggt
     8761 cgtgccggcg cagatgacaa tcatgccgcc ggtcgcacag acgaactcgg aggtcggcag
     8821 cgtcgcctcg cccggatgtt cgaacacgat cttcgggccg acgcgttcgc cgagggcgtc
     8881 ccagaccgcc ttgccgaagg cgcgcgcgcc cttggcccac tcgccgtacg cccgggcgtc
     8941 ggtcgtgtcg ggcattttcc cccagtgggt gaagcccgtg cggttgacga cgccgacggc
     9001 gccgtgatcg acgcagaact tgcgcttgtc ctcgtcggag acgacggcga tcgggcggcc
     9061 gccgagcgcc cgggtgatct cgagcgccat gctgcccagc ccgccggcgg cgccccacac
     9121 gagcacgatg tcgccgggct cgacggtgtg cggcggccag cccatcagca tgcggtaggc
     9181 cgtggagccg cagagaagga agcaaccggc ttcctcccag gtgaggcgcc tgggcttggg
     9241 caggcactgg tgaccctgtg cccgggcgaa ctggcagtag ctgccgtagt tcgtctggta
     9301 gccccagatc cgcgtgctct cggccagcat cggatccttg ccggcacgca cccacggatc
     9361 gtcccgccgc caccagccgc tgtggacgat gacctcgtcg ccgacggcga cggtgtcgac
     9421 gtccctgccg acggcccaca cgatgccgga gcagtcgctg ccgccggcgt ggaagtcctc
     9481 gggctcgccg gtcttctgcc gctcggcgat gacgtcgagc ggatagccga gcgcggccca
     9541 cacgttgttg tagttgatgc cggaggccat cacgtagatc agcacctcgt cggcgccgag
     9601 ggcgggggtg gagatcacct cgcgcttcca tgcgtcgcgc ggctgaccga agcggctctg
     9661 ccggacgacg taggcgtgca tcttcgccgg cacctcgccg agcggaggcc ggtcgccgag
     9721 gtcgtagagg tctttcatgg atcgggctcc ttcagccgtt gaattgcttc acgacgcggg
     9781 ccaggcgcgg gtcgtcgagg ccgccccggt gctgcggctc gtagaacgcc gtgcggtcga
     9841 gcaggcccgc gtagcgctcc tggagcgccg cggcgatggt gtcgtaggtc ccggtgacgg
     9901 cgtaggtctc caccatctcg tcggtgacga ggctcgccat gctcacccag tcgccctcca
     9961 ccgatttgcg gtgcaggtga gggacgaggt cttgccagcc gtgggcggcg agcacgggct
    10021 cgtaggtgcg cgtggaggca tagaaggcga tctgctgacg gacggcctgg cgggccctgg
    10081 cgcgttcctc ctcggtgtcg ccgatgacca cgaagctcgc cgtggagtac gtgaagtcct
    10141 cacgcgtgcg ccccgacgcc cgcaggccct cctcgacggc cgggtgcacg tactcgcgca
    10201 ggtacttcgc cgagttgaag ggatggacgt ggaggccgtc gcagacctcg ccggccacgc
    10261 ggcagatgta gcggttcacg ccggcgatgt agatggggat cttcgggtgg tcgatggggc
    10321 ccgggctgaa gaacggcgtc atgagatcga agcggtagaa ctctcccttg aagttcagct
    10381 tcgtcccgtt ctgccagcag tcccagatgg cgcgcagcgc ccgcacggcc tcgcgcatct
    10441 tcggccccgg ggattcgaac ttcaccgaga accgacgctc gttgtggccc ttgacctgcg
    10501 tgccgagccc caggatgaag cggccgtcgg aggccttctg gagatcccac gcgatgtgcg
    10561 cggtgatcat ggggctgcgc gggaagacga cggcgatggc ggtgccgagc ttcatgcggg
    10621 cggtcaccgt ggccgcgacg gccagcggca ggtaggggtc gtgctgggtc tccgaggacc
    10681 agaggcaatc gaagccgagc gcctcgacct tgcgggcgta ggcgggaatc gacttgaggt
    10741 cgtgggtggg catgccgacg tcgagcttca cggcctggtc tcctctcgca gggttcgccc
    10801 gcgtatctta cctcggccgg cgatcgatga ccgcccgcgc ctccccgacg atcgtctgca
    10861 ctccctccgt ggcgccgggc aggtcgcgga cgagcccaac gctgggcccc cccacatcgg
    10921 gtagtactcg ccggtctgcc gggccgcgga ggcgttgtag acgtcctggg cgatggcgcg
    10981 gcggagcctc ggcgggagca cgggcgcgcc ggccgcggcg tgctcccggc tacgcagtct
    11041 catgaatcgt gtctccttcg cgggtcccgg cggccgggcc actccaccgc gccgaggaag
    11101 cacggtgcca gctcccgggc tcacggctac tcgatgcgga agaggcgcgc ggcgttccgg
    11161 tgcgccacgc tttcggcggc gtccggaggc agctcgccca ggatccggcg agtccacttc
    11221 atcaggcggt ccagcatgac cggctcgagg agccgaggcg accacacgtc gatcccgatc
    11281 aggaaccggt cgggcatctt ctctatcagc gttttccagt cgcggtcgag cggcccggtc
    11341 tcggtggcga gcgcgggctt gcgctggaag tgcatacccg acagatcgac catcaggttt
    11401 gcgttccgct cgagcaggcc ctggacgcgg gccggcggtc cctctccggc gtgggcgagc
    11461 acgaccgtcg ccttggggtg ggcggccagc gcgcggtcga gcgcggcggt ggcctgatcg
    11521 gtcagctcgt agtggatgac gaggggcacc ccgtgcttcg caacaatttc cagcacgcgg
    11581 ccgaaggccg ggctggccgg gtcgcggtcg atgttgcggc tgcccacctg gcgtacgtgc
    11641 acctcaccgg ccacgcggcc acgccgggcg accagcgcct cgtcgagcat cctggctccg
    11701 gtcgcgctca tcgggtccgg taccggtaca gcggcgacga ccaggtcggg atacttcttc
    11761 gcggccccgg tgatccacgc atcatcgtcc ctctgcgccc cacccacgcc gagcagcacg
    11821 accttcttga cgttgtggcg tttcgccgcc ccggcgtacg cgtcgatggc cttggagctg
    11881 gagacgtgcg agtgtgcgtc gatgatcggg ccggcgtagt cggcggccca caccggcgcc
    11941 gccaggacga gaacggcgag aacgagagcg cgcacctact tcgcctcctt cggcaccggc
    12001 agcatcgtcc ggaccagacc ggtggagttg atccacatct cctgggtcac ctcggtgggc
    12061 acatgtgaga tccctacgac ccggacgaga tcgaccatgc tgaccggcgg gtgtcttccc
    12121 ggatcttgcg gtgctacttg aggttcttga agtgctgggc cagggcgtcc atcacgcggg
    12181 cgagggcctc cttctcggtc agccccttgg ccgtcacctt gtactccacg gcctcggcga
    12241 tccagtcgcc ggcctcgttc cggtacacct cgacctcgaa ctgcacgggt ccgagcttac
    12301 cccagaatcg ccgttgacgc cgcctcgtcg tgacgaccag catcccgcat ccgccactcc
    12361 cggcgcgagc gggtggaccg ggcgcagccg ccgtgcggag gcgggtcacc gggccgccgg
    12421 gctcagcccc acacctcgcg cgcggcggcg accacacgct cgagcttctt ccgctgatcg
    12481 tcctccgtca cgcggttacc ctccatggtc gaggcgaacc cgcactgcgg cgagagggcc
    12541 aggcgctcga gcggcaccga ggcggcggct tccgtgatcc gcgacttcag cgtctcgagc
    12601 ggctccagcg ccggtttctt ggtgctcacg agccccagca ccaccacgcg gtcgtcgggc
    12661 accgcgcgca gcgggccgaa gccgcccgac cgctcgtcgt cgtactcgag caggaagcgc
    12721 tggaaccgcg tccggcggaa gacgcgctcg atgggatcgt agccgccgct cgcgtagaac
    12781 atcgactggt tgttgccccg gcagatgtgc agcccgaacg tgatgcccgg gtgtccggcg
    12841 atgacggcgt tgtccatgtc cacgcaggcg tcgagcatcg tgtctggatc cgcgccccgc
    12901 tcgacgtagc cggcgcggat cttcgggtcg agcagcgcgg cgtactgcgg cgcgtcgatc
    12961 tgcacgtacg tgcagccgag ccgggccagc tcctccacct cgcggcgggt gaagtccacg
    13021 acgtcggcga ggtacgcgtc gcgtgtgggg taggcggcgg ccgacttgcc cgggtcccag
    13081 taggcggcgg cctgctgggc gctgatcagc gtcaccttga ccggccggcg cgcgcgggcc
    13141 cgcccgtaca cgaactcctc ggcgcacatg gaacgccgcc agcgcagccg ctcgacgacg
    13201 acgggccgct tgaagaccag ttcctcaccc tgctcgttgt ggaaggggat ggcccagccg
    13261 ccgaagcggt cgaagccgtc gagagcctcg acgaggtggc cgaagaaggc gtagcggcgc
    13321 agctcgccgt cggtgaccac gtcgagcccg acctgctcct ggagcgcgat cgcctcgtcc
    13381 accgcgcggt cctccacgcg cttgaaggcc gcggcgtcga gctgtccggc ctcgcggtcg
    13441 gcgcgggcct tcacgagggc tgccgggcgc aggaggctgc ccaccacgtc cgtgcgccag
    13501 gccgtcacgg cggccgccgt cactgcggca gcgggttcac cggatcccac gggtacacga
    13561 tccatccgtc ggtctcttcc gcgtgatggt cggggccgtc gcccgggaac ttgctgtggt
    13621 gcggcttgta gtggagcacg cagacgtcca cggcggcgcc ggccttgcgc agccggtcgc
    13681 ggacggcgac cacggtgctg ccgctgtccc agacgtcgtc caccacgagg atgcgccggc
    13741 cttcgacgtg gcggtcgtcg gggaattgca cgaaggccgg tgcgggcaac gcgcgctcct
    13801 cgccgtcgta gagcacgacg gccgcgcaca ggatgttgcg gatgccggtc cgctcgctga
    13861 tgaggcaggc cggcaccatg ccgccgcggg tgacgaccag gatgttgtcg tagtcgcgcg
    13921 acagcgtgcg gaggagcttg ccgaccatgc gctcgacgtc gctccagctg acgtggtgct
    13981 tttccaagcc ccgtgctccc tccgcgggcg actcgcgttg cccttcttct accacaagtc
    14041 ggtcacgcga agcgccagcg caggagccgc tcggggatca cgcggatcag cgttccgtgc
    14101 gctcggtcga gcgccatggc gcggtactgc gggtacttcg ccatcagcag gtcgatcgcg
    14161 cccgtgtact cgacgccgtc cgtcagcacg tcggcgcggc cctcgacgat gacccagcgc
    14221 aggcgcgtcc agtcctcgtc gtactcgtcc accaccagcg acacgcgcgg gttctcggcg
    14281 atgttgcgga gccggcgcag gtcccgtgtc cgtttgggct tggcgtcgat caccgagaag
    14341 aggcgggcgc cgtcgaaggc gtagcacacc ggcaccacca gcggccggcc cgacgcgtcg
    14401 gccgtcccca gccgggccac ccgcgcctca cggaggaggt cgagggccca gccggggacg
    14461 tccgcgatca ccggaggagc ttcgcgtgct ggtcgagcag cggcagcacc ttctcccgcg
    14521 gctccgaggg cagccggatg atggcgcgcg tcaggcccgc ctgccggtaa cggtcgagcg
    14581 tcgccgcgtc gccgggggcg ccgaacaccg agaccgagat ggtcttcatg tcgcgccccg
    14641 ccctcgcggc gcgcgtcttg aggtcctcga gccccgcgaa gatgcgctcg gcgctgcgcg
    14701 cccgagggaa ccagccgtcg ccgatctcga ccacccgctg cagtgtgtgc tcggtctcgc
    14761 cgccgaacag cacgggggga tgcggcttct gcacgggctt gggatacatc cagatcgggt
    14821 cgaagttgac gtgctccccg tggaactcgg ccgtctcctc cgtccagatc gctttcatcg
    14881 cgcgcacctg ctcgcccagg cgcttgaagc gcgtcttgaa gtcggtgccg tggttctcca
    14941 tctcgtcctg gttccagccg ccgcccacgc cgagcaggaa gcgcccgttg gacacgaagt
    15001 ccagggaggc gacctccttg gcggtcgtga tcgtgtcacg ctggatgagc aggagaatgc
    15061 ccgtgccgag cttcaggttc ctggtcacca tcgccgccgc ggcgagggcg acgaaggggt
    15121 cgagggtgtg cttgtactcg cggggcagct cgccgccgct cgggaagggc gtcttgcggc
    15181 tcaccgggat gtgggtgtgc tcgggcaccc agagcgactc gaagccgcgc tcctcgcacg
    15241 cccgggccag gtcctcgatg cggatggcgt agtcggtgtt gaacatgcag acgccgatgt
    15301 tcataaggcc tcctctggcc gctggagttg ccgtactgta cctcagcccc cgccgggggt
    15361 gagcgagaag tggaggcgac gacctccgtg ccgggggccc gcacgtagaa gtcgcaggtg
    15421 gccgcgaggt cgcggacggt gagcacgagg tggtcgaccc aggtcgatga tcagacgaag
    15481 atgctctcgg ggaggaaggc cgacacgatc cagttgggcg cgtccacgat ctcgctgatc
    15541 ctgaggtcca cggccacact gcagaggatg taggcctcct cgcgcgacag cccgcgctcc
    15601 tcgacgaggt ggtcgatcat gtagcggatg gcctgttgcg ccgagcgata gaggtcgtgg
    15661 ccgtgagccg tcgtggcgaa gtgcggaccg gtgttggtcc tggccccgac gggaccgcgc
    15721 gtccggaagc gcggctcggc cagtcggcgg cccggctgca ggtcgaagcg ggccgtcacc
    15781 tgcgccatca tctcgatcgc ggtgatgcac acctcgccgt cgccctgcgc gccgtggccg
    15841 tccccgacgg agaacagcgc accgtccacc cagacgggca ggtagacggt cgtgcccgcg
    15901 gtgagctgct tcacgtccat gttgccgccg ttcttgcgcg gcggcatcgt gctgtgaccg
    15961 cccggctcct cgagcgcgac gccgatgacg cccgggaacg gatcgatggg cacggcgatc
    16021 cgcgcgccca agcgcgcgag gagaccgtcg gagagatccc agatcgccag gtacggcttg
    16081 gggaagtcgt cctcgggcag cagcccccgg ccgggccgga tgttcgtcca gccgaagggc
    16141 gc
//
LOCUS       DATIAZ010000372         6199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1306868, whole genome shotgun sequence.
ACCESSION   DATIAZ010000372 DATIAZ010000000
VERSION     DATIAZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306868"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VLF19_12735"
     CDS             complement(<1..271)
                     /locus_tag="VLF19_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSE94167.1"
                     /translation="MSVTVGVSVKRREDARLLGGAGRYLDDLRVPGMLHLAIVRSPHP
                     HARIVSVGRRAARACPGAVAVFTVDDLPELGGSVPPLVPAPAIRAY"
     gene            428..1249
                     /locus_tag="VLF19_12740"
     CDS             428..1249
                     /locus_tag="VLF19_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSE94168.1"
                     /translation="MAALAAIFLLLLAPVAASAADLPIFDAHVHYSHDAWEVLSPKEA
                     VALLRKGGVVRALVSSSNDEGTQKLHAEAPDIVIPELRPYRRRGETGTWVRDESVITY
                     LEERLRKHRYVAIGEFHLSGADAELPVVRRMVELARQYGVMLHAHSDADAVERLFRLD
                     PGARILWAHAGFEPPSRVREMLRKHRNLWADLAFRSDHAPGGKLAPEWRAAFVEFPDR
                     FMVGTDTFVPERWQHVPEHAAWSRGWLAELPPDVAERIAWKNGEAVLGAAFSRPR"
     gene            complement(1297..2238)
                     /locus_tag="VLF19_12745"
     CDS             complement(1297..2238)
                     /locus_tag="VLF19_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSE94169.1"
                     /translation="MPELPDVEAYLEALRPRVTGARLAGVRLATPFLLRSVEPPLTEA
                     CGLTVSGLRRLGKRIVVALEGQLFLVLHLMIAGRLHWKPGSARIPGRIGLAAFDFSTG
                     TLILTEAGTKRRAALWAVRGEAALRDHDPGGVEPLDAGLDAFRTVLGRERHTVKRTLT
                     DPRFFSGIGNAYADEILHRARLSPVRMTEQLTDEEVARLHAATRATLTEWVERLRAEA
                     RDRFPEGVTAFRPGMAVHGRYGQPCPVCGAAIQRIVHADNETNYCARCQTGGRLLADR
                     ALSRLLRSDWPRTLEEMEERRAAAAGHGLAAPPKRRV"
     gene            complement(2231..3295)
                     /locus_tag="VLF19_12750"
     CDS             complement(2231..3295)
                     /locus_tag="VLF19_12750"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSE94170.1"
                     /translation="MPSATLQPQEHFPLAPFCTLGVGGPARFFVEARDEAAVVDALGW
                     ARRRRVPLRVLGGGSNLVVADEGVDGLVVRVATRGIELREDGGAVTLTAAAGEVWDDI
                     VRRAVERGWAGIECLSGIPGLVGATPIQNVGAYGQDVSETVVSVRALDTTTSRAVGLT
                     AADCGFTYRDSVFKSGEPDRYVVLAVTYRLRPGAPPSVRYTEVAAHLERAGVESPTLA
                     QVRETVVAIRRSKSMVIEDGDPNRRSCGSFFTNPVVSAAEAERVAALAGDAAMPRWPQ
                     PGGGTKLAAAWLIERAGFRRGHREGTVGLSTRHALAIVAHDGARAGDVLAFARRVQTA
                     VEDRFGVRLAREPVLWSDGA"
     gene            3349..4719
                     /locus_tag="VLF19_12755"
     CDS             3349..4719
                     /locus_tag="VLF19_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSE94171.1"
                     /translation="MALPVGRSADPAAGVRASAAEPRPALRTIDATALIVGTVVGAGI
                     FRTPSLVAANSGTESAMLLAWVAGGLISLVGALCYAELAAAYPHPGGDYHYLGRAFGR
                     RLAFLFAWARISVIQTGSIALLSFVFADYATDILPLGPFSSPLYAALCVAALTALNVA
                     GVRQGKTVQNLLTTVEVLGLALVVGAGLLVTPAAGPAAAAPTAGSTAFGLVMIFVLLT
                     YGGWNEAAYISAEMHGRRDIVRALVWSLLVVTVLYVLVNWAYLRGLGLQGVAGSRAVA
                     ADLLERSFGVGAGRLVGVLIAVAALTSANATIFTGARSAYALGRDFRVFALLGRWHPG
                     PRTPVNALLAQGVAALVLVLLGAATRKGFETMVEYTAPVFWLFFMLTGVALFVLRRRE
                     AATPRPFRVPLYPLTPVLFCATCAYLLYASLAYTGVGALVGVAVLAVGGVVLALSRGR
                     EAQGGS"
     gene            4721..5302
                     /locus_tag="VLF19_12760"
     CDS             4721..5302
                     /locus_tag="VLF19_12760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE94172.1"
                     /translation="MPVHRVLTALLAVVLLGCAAGVGAQAPATTPKPLDVPFVPTKEP
                     IVDQMLKMAGVKTGDVVYDLGCGDGRIVITAAKRFGIRGVGIDLDPERISEANANALK
                     AGVADRVKFIQGDLFDAKIDEATVVTLYLLPDVNLKLRPKLLSELKPGTRIVSHNYDM
                     GDWKPEQTVKLTVDGTEHIVYAWTVPKRAARNQ"
     gene            complement(5330..5929)
                     /locus_tag="VLF19_12765"
     CDS             complement(5330..5929)
                     /locus_tag="VLF19_12765"
                     /inference="COORDINATES: protein motif:HMM:NF022060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HSE94173.1"
                     /translation="MRMIPADPIAEIVAERERARAAGDPLADVCLLATSGGVGAPGVR
                     PLILRDVGPDGVGLLVSATSPKWEPLRGGRYEGLLLWTSVRRQYRVRGGLAPMPEPLV
                     ERYWSQKVHESRLLDVYYETAQPQSSVVASHEAFRSGIEALRARHPTPDAVPRPPLLR
                     GVYLVPFRVEVWHGALDRLHDRRAYVKTPAGWRVDVLVP"
     gene            5980..>6199
                     /locus_tag="VLF19_12770"
     CDS             5980..>6199
                     /locus_tag="VLF19_12770"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HSE94174.1"
                     /translation="MAYTDHEQTIVTVRGWIDRARRIVVLTGAGISTDSGIPDFRGPQ
                     GVWTKNPAAEKMATLQHYVADPEVRRRSW"
BASE COUNT          803 a         2266 c         2273 g          857 t
ORIGIN      
        1 cgtacgcccg gatcgccggc gccggcacca gcgggggaac cgacccgccc agctcgggca
       61 gatcgtcgac ggtgaacacg gcgacggcgc ccgggcacgc gcgcgccgcc cgccgtccga
      121 cggacacgat gcgagcatga ggatgcgggc tcctgacgat ggcgaggtgg agcatcccgg
      181 gcacccggag gtcgtcgaga taccggccgg cgcccccgag gaggcgcgcg tcctccctgc
      241 gcttgacgga gacgccgacc gtcacggaca cgtggccgcg gccgtcagcg ggcgggcgcg
      301 acctgaacga aggtggagtt gaaggggctg gccccggccg gcgacgcgcg gtcagcgatc
      361 agcattgcca tcgcgtgtat tctagtcgcc agccggattc ctggcacgct cggtggaggt
      421 gcgacagatg gccgccctgg ccgcgatctt cctgctgctc ctcgccccgg tcgccgcgtc
      481 ggcggcggac ctgccgatct tcgacgccca cgtccactac agccacgacg cctgggaggt
      541 gctgtcgccg aaggaggccg tggcgctgct ccgcaagggc ggcgtcgtgc gggcgctcgt
      601 gtccagctcg aacgacgagg gtacccagaa gctccacgcc gaggcccccg acatcgtcat
      661 ccccgagctg cgtccctacc gccgccgggg cgagaccggc acgtgggtgc gcgacgagtc
      721 cgtcatcact tacctcgagg agcgcctgcg caagcaccgc tacgtcgcca tcggtgagtt
      781 tcacctctcc ggcgccgacg ccgagctgcc ggtcgtgcgc cgcatggtcg agctggccag
      841 gcagtacggg gtgatgctcc acgcccactc ggacgccgac gcggtggagc gactcttccg
      901 gctggacccg ggcgcgcgca ttctctgggc gcacgccggc ttcgagccgc cgtcccgggt
      961 gcgggagatg ctgcgcaagc accggaacct gtgggccgac ctggccttcc gcagtgatca
     1021 cgcgcccggc ggcaagctcg cgcccgagtg gcgcgcggcg ttcgtcgagt tccccgaccg
     1081 cttcatggtc ggcaccgaca ccttcgtgcc cgagcgctgg cagcacgtcc ccgagcacgc
     1141 cgcgtggtct cgcggctggc tcgccgagct gccgccggac gtcgccgagc gcatcgcctg
     1201 gaagaacggc gaggccgtgc tcggcgccgc tttctcccgg ccgcgctagc gggagatcac
     1261 ctcgacgctg gcgcggcggc gccgacgctc gccggcctac acccgccgct tcgggggggc
     1321 ggccagtccg tggccggccg ccgcggcgcg ccgctcctcc atctcctcca gcgtgcgcgg
     1381 ccagtcgctg cgcagaaggc gcgagagagc gcggtcggca agcaggcggc cgccggtctg
     1441 gcaccgcgcg cagtaattgg tctcgttgtc cgcgtggacg atgcgctgga tggccgcgcc
     1501 gcacaccggg cagggctggc cgtagcggcc gtggacggcc atgcccggcc ggaacgcggt
     1561 gacgccctcg gggaagcgat cgcgcgcctc ggcgcgcagg cgctcgaccc actccgtcag
     1621 cgtcgcgcgg gtcgccgcgt gcagccgcgc gacctcctcg tcggtgagtt gctcggtcat
     1681 gcggacgggg ctgagccgcg cacggtgcag gatctcgtcg gcgtaggcgt tgccgatccc
     1741 ggagaagaaa cgcgggtcgg tgagcgtgcg cttgaccgtg tgacgctcgc gccccaggac
     1801 cgtgcggaac gcgtcgaggc cggcgtcgag cggctcgacg ccgccggggt cgtggtcgcg
     1861 cagcgcggcc tcgccgcgaa cggcccagag cgcggcgcgg cgcttcgtcc ccgcttctgt
     1921 caggatcagc gtgcccgtcg agaaatcgaa cgcggcgagg ccgatccgtc ccgggatgcg
     1981 agcgctgccg ggcttccagt gcaggcggcc ggcgatcatc aggtggagca ccaggaacag
     2041 ctggccctcg agggcgacga cgattcgctt gccgagccgg cgcaggccgc tcaccgtcag
     2101 cccgcacgcc tcggtgagcg gcggctcgac cgagcgcagc agaaacggcg tcgcgagccg
     2161 cacgccggcg agccgtgcac ccgtcacgcg cgggcgcagc gcctcgaggt aggcctcgac
     2221 gtcgggcagc tcaggcaccg tcgctccaga ggacgggctc gcgcgcgagg cgcacgccga
     2281 agcggtcctc gaccgccgtc tgcacgcgcc gggcgaaggc gaggacgtcg ccggcgcggg
     2341 cgccgtcgtg cgccacgatc gccagcgcgt ggcgcgtgga caggcccacc gtcccctcgc
     2401 gatggccgcg gcggaagccc gcgcgctcga tgagccacgc ggccgccagc ttcgtgccgc
     2461 cgcccggctg cggccagcgt ggcatcgcgg cgtctccggc gagcgccgcg acgcgttcgg
     2521 cctcggcggc ggagacgacc gggttggtga agaaggagcc gcacgagcgc cggttggggt
     2581 cgccgtcctc gatcaccatc gacttcgagc gccggatggc caccaccgtc tcgcgaacct
     2641 gggccagcgt ggggctctcc acgcccgcgc gctcgaggtg ggccgcgacc tccgtgtagc
     2701 ggacgctcgg gggcgcgccg ggtcgaaggc ggtacgtgac ggcgagcacg acataccggt
     2761 cgggctcacc gctcttgaag accgagtcgc ggtaggtgaa gccgcagtcg gccgcggtca
     2821 acccgacggc acggctcgtc gtcgtgtcga gcgcgcgcac gctgacgacg gtctcggaga
     2881 cgtcctggcc gtaggcgccg acgttctgga tcggcgtggc gccgaccagg ccgggaatgc
     2941 cgctcaggca ctcgatcccg gcccaccccc gctcgactgc gcgccgcacg atgtcgtccc
     3001 acacctcccc ggcggccgcc gtcagcgtca ccgcgccgcc gtcctcgcgg agctcgatac
     3061 cgcgggtggc cacgcgcacc acgagaccgt ccacgccttc gtcggcgacg accaggttgg
     3121 agccgccgcc caggacgcgt agcggcacgc ggcgacgccg cgcccacccg agcgcgtcga
     3181 cgacggcggc ctcgtcgcgc gcctcgacga agaagcgcgc ggggccgccg acgcccaggg
     3241 tgcagaacgg tgcgagcggg aagtgctcct ggggctgcag ggtggcgctg ggcatgcttc
     3301 ggagcgagtc tacacccggg tcttgcgttt cctgcgatac tcacccccat ggcccttccc
     3361 gtggggaggt ctgccgaccc cgcggccggc gtgcgtgcgt cggccgccga gccgcgtccg
     3421 gcgctccgca ccatcgacgc caccgcgttg atcgtcggca ccgtggtggg cgccggcatc
     3481 ttccggacgc cctcgctcgt ggccgcgaac tccggcaccg agtcggccat gctgctcgcc
     3541 tgggtggcgg gcggtctgat ctcgctcgtg ggcgccctgt gctacgcgga gctcgcggcc
     3601 gcctatccgc accccggcgg cgattatcac tatctcgggc gcgcgttcgg ccgccgcctc
     3661 gcgttcctct tcgcctgggc caggatcagc gtgatccaga cgggctcgat cgcgctcctg
     3721 tccttcgtct tcgcggacta cgcgacggac attctgccgc tggggccctt ctcctcgccg
     3781 ctgtacgcgg ccctctgcgt cgccgcgctg acggccctca acgtcgccgg cgtccggcag
     3841 ggcaagaccg tgcagaacct cctgaccacg gtcgaggtgc tcgggctggc gctggtcgtc
     3901 ggcgccgggc tgctggtcac gccggccgcg gggccggccg cggccgcccc gaccgccggc
     3961 tcgacggcgt tcggtctcgt gatgatcttc gtcctgctca cctacggcgg gtggaacgag
     4021 gcggcgtaca tctcggccga aatgcacggc cgtcgcgaca tcgtgcgggc gctcgtctgg
     4081 agcctcctcg tcgtcaccgt gctctacgtg ctcgtcaact gggcgtatct ccgcggcctc
     4141 gggctccagg gcgtcgcggg gtcgcgcgcc gtcgccgccg acctcctgga gcggtcgttc
     4201 ggggtgggtg cggggcgcct ggtgggggtg ctcatcgccg tggccgccct gacgtccgcc
     4261 aacgccacga tcttcaccgg cgcccgcagc gcctacgcgc tcggccgcga cttccgcgtg
     4321 ttcgcactcc tgggccgctg gcatccggga ccccgcacgc ccgtcaacgc gctgctcgcc
     4381 cagggcgtgg cggcgctggt gctggtgctc ctgggcgcgg ccacgcgcaa gggtttcgag
     4441 acgatggtcg agtacaccgc gccggtgttc tggctcttct tcatgctcac cggcgtggcg
     4501 ctcttcgtgt tgcggcggag ggaggcggcc acgccgcgcc cgttccgcgt cccgctctat
     4561 ccactgactc cggtgctgtt ctgcgcgacg tgcgcgtacc tgctctatgc cagcctcgcg
     4621 tacacggggg tcggcgccct cgtgggtgtc gcggtgctgg ccgtgggcgg cgtggtgctc
     4681 gcgctctcga gaggccgcga ggcccaagga ggaagctgag atgcccgttc accgcgttct
     4741 caccgcgctg ctcgccgtgg tcctgctggg gtgtgccgcc ggcgtcggcg cccaggcgcc
     4801 cgccaccacg ccgaaacccc tcgacgtccc cttcgtgccg acgaaggagc ccatcgtcga
     4861 ccagatgctc aagatggccg gcgtcaagac gggtgacgtc gtctacgacc tcggctgcgg
     4921 cgatggacgc atcgtgatca cggccgcgaa gcgcttcggc atccgtggtg tcggcatcga
     4981 cctcgatccc gagcgcatct ccgaggccaa cgcgaacgcg ctcaaggccg gagtcgccga
     5041 ccgcgtgaag ttcatccagg gcgacctgtt cgacgccaag atcgacgagg cgacggtggt
     5101 gacgctctac ctgctgccgg acgtcaacct caagctgcgg cccaagctgc tgagcgagct
     5161 caagccgggc acgcggatcg tctcgcacaa ttacgacatg ggggactgga agcccgagca
     5221 gacggtgaaa ctcaccgtgg acgggaccga gcacatcgtc tacgcctgga cggtgccgaa
     5281 gagggccgcc cggaaccagt gaccccgggg ggccggggcg cctgggctac taggggacga
     5341 ggacgtcgac gcgccagccg gcgggcgtct tgacgtaggc gcggcggtcg tggaggcggt
     5401 cgagggctcc gtgccagacc tcgacgcgga agggcacgag gtagacgccg cggagcagtg
     5461 gcgggcgcgg caccgcgtcg ggcgtcgggt gccgtgcgcg cagcgcctcg atgccggacc
     5521 ggaacgcctc gtgcgaggcc acgacgctgc tctgcggctg cgcggtctcg tagtagacgt
     5581 cgagcaagcg cgactcgtgg accttctggc tccagtaccg ctccacgagc ggctcgggca
     5641 tgggggcgag cccgccccgc acgcggtact ggcgccggac ggacgtccag aggagcaggc
     5701 cctcgtagcg cccgccgcgc agcggctccc acttcgggct cgtggcgctg acgagcaggc
     5761 ccacgccgtc ggggccgacg tcgcggagga tgagcggccg cacgccgggc gcgccgacgc
     5821 cgccggaggt ggccaggagg cagacgtccg cgagcggatc gccggccgcg cgtgcccgct
     5881 cgcgctcggc gacgatctcg gcgatcgggt cggccgggat catgcgcatg cgcgcagcat
     5941 atacaatcac cgcggcgccg gtcgggtaga atcgccgcca tggcgtacac ggaccacgag
     6001 cagacgatcg tcaccgtgcg cggctggatc gaccgggcgc gccgcatcgt cgtgctcacc
     6061 ggcgccggca tctcgaccga ctccggcatc cccgacttcc gcggaccgca gggcgtgtgg
     6121 acgaagaatc cggctgcgga gaagatggcg accctgcagc actacgtggc cgatcccgag
     6181 gtgcgcaggc ggtcgtggc
//
LOCUS       DATIAZ010000373         6156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_387845, whole genome shotgun sequence.
ACCESSION   DATIAZ010000373 DATIAZ010000000
VERSION     DATIAZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387845"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /gene="rpsU"
                     /locus_tag="VLF19_12775"
     CDS             complement(<1..107)
                     /gene="rpsU"
                     /locus_tag="VLF19_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HSE94175.1"
                     /translation="MTKVIVEPDESFESALKRFKKQCEKAGLLSEFKKR"
     gene            320..793
                     /locus_tag="VLF19_12780"
     CDS             320..793
                     /locus_tag="VLF19_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HSE94176.1"
                     /translation="MTASILLFVACFALWLVLSGHFGLLEIALGVASAAAVTAANRNL
                     EALSPFLRVAPRLLAYGVWLLREIVVANLQVTRVVLHPRMPIEPVVSRFEVTLPGDLA
                     IATLANSITLTPGTITLDIEGRALVVHALLGPASVLAAEGEMARRVGRVFAGRMA"
     gene            790..1065
                     /locus_tag="VLF19_12785"
     CDS             790..1065
                     /locus_tag="VLF19_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HSE94177.1"
                     /translation="MTPEGLFLAMTLGLLGLVTVYLYRVAAGPTIFDRILGLSGFGTK
                     TTVVLLLVGALYARLDMFVDLAFAYALLSFVGSLAAARYFERGGPHR"
     gene            1062..1370
                     /gene="mnhG"
                     /locus_tag="VLF19_12790"
     CDS             1062..1370
                     /gene="mnhG"
                     /locus_tag="VLF19_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HSE94178.1"
                     /translation="MTIVIAILLAGGFFFQAVAALGVIRLPDFYTRLHAVSKAETLGV
                     LLTLAALVVWTGPSLVAVKVGFVGLFMFLANPTSTHAIGRAAFRTGVRPWLRDPEEGR
                     "
     gene            1367..1891
                     /locus_tag="VLF19_12795"
     CDS             1367..1891
                     /locus_tag="VLF19_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HSE94179.1"
                     /translation="MSEVVVAVLLVFLGVLAVVAVRAADVLLAAIALGVYSFVMALTW
                     AALGAVDVSFTETVVGAGASTVFFLAALLRTSRRRSPAPGRFRWAALAVVAAVGVLLI
                     GATTDLPPFGDPAAPPSVHVSPRYLERTREETRTPNAVTAVVADYRGYDTLIETVVIF
                     TGGLAGSLLLARPR"
     gene            1888..2325
                     /locus_tag="VLF19_12800"
     CDS             1888..2325
                     /locus_tag="VLF19_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HSE94180.1"
                     /translation="MIERHDSPVVTSFVRVLVPISQLFGLYVLVHGHESPGGGFQAGV
                     ILAASYIVLALGLGREALDRRVNEPWCLALGALGVLIYALTGVVGVVRGRAFLDYSAL
                     PLPVSPAEARYFGILFVETGVAIAVAATIVVLFCRLADREHAP"
     gene            2322..2687
                     /locus_tag="VLF19_12805"
     CDS             2322..2687
                     /locus_tag="VLF19_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HSE94181.1"
                     /translation="MSAPYWMAVALLMLGLYVMIAHQNLFKKFVGMTIFQTAIILFFL
                     LLSVKRHGDLPIVPNGPVSSESFMNPLPHALMLTAIVVAAATTGVALAILIRLHAAYG
                     SLEEDVVTGHAVRESSPDS"
     gene            2684..4120
                     /locus_tag="VLF19_12810"
     CDS             2684..4120
                     /locus_tag="VLF19_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSE94182.1"
                     /translation="MTDHLAVLPLVIVLVAALLQPIVGLAGRRAAWPLAVAATAVATL
                     AALVGLGAVLTRGTLRYAVGGWDPPWGIEVVLDPLSGFAASVILGVATLSLLGSGPLV
                     RARHRSEEPVYYALALLMLTGLTGIVISGDLFNVFVFLEVASISSYALVASGGGPALA
                     AGFRYLVLGTVGASFYLLGVGLLYALTGTLNMADLAARLPAVTGSSPGAGGMALIVAG
                     LALKMGLFPLHGWLPDAYTYSSPSVASLLGPVATKAAAYALARLLLYVLAARETPVAT
                     IVAWAGAASIMAGGVMALRQVDPRRLLAYSSVSQIGYIALGIGLANASALSGAYLHVL
                     NHAVLKATLFIAVGASASDPPAGMPTWRKPITAACAVVAALSMVGVPPVGGFFSKWYL
                     LEGALEARQPIFVAVILAGSLLAVGYTYRLTEAVWFEGSRADGRREEAPPSVLIGLVL
                     LAVLAVAAGLANSVIVDAILLPAVAGAR"
     gene            4120..5598
                     /locus_tag="VLF19_12815"
     CDS             4120..5598
                     /locus_tag="VLF19_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSE94183.1"
                     /translation="MDVASTLPGWALVPPAAATALIAASRRRPVVREAWSVAAAIAQS
                     AIVAVMLPRVLAGERLVWEPVSLAPGVPLLLRVDPLGVLFALLASALWVVTTVYSIGY
                     TRALTEPRQTRYFASFALSLLAAAGVAMAGNLLTFFVFFEILTVATYPLVVHRGNAEA
                     ARAGRIYLGYTLGGGTALLGAVAWTQVLAGRLDFQPGGMLHATAASPSQLWGLFGLFA
                     IGVGVKAALVPLHSWLPVAMIAPTPVSALLHAVAVVKAGVFGIVRVVGFVFGIDLLQD
                     LGAAPVLAGIAGATIVLGSLAALAQDNLKRLLAFSTVSQLSYIGLGASLGTPDAMAGA
                     VMHIASHALLKITLFFCAGAIYATLHLERVSELDGVGRRMPLTMGAFATAAFMLAGLP
                     PGPAFVSKWYLLTGAVAASAWWAVGVLLASTLLNLAYFTPIVVRAFWGRGAGPSQEAR
                     PALLIPLLATAGAAIVLGLAPEAPGGLLALARDVATGTIAAR"
     gene            5595..5786
                     /locus_tag="VLF19_12820"
     CDS             5595..5786
                     /locus_tag="VLF19_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94184.1"
                     /translation="MMRVLAALSGLLILLVALEIALVPEPHRVRGAFAVIGLVSCVTI
                     VLVSKLLGKVWLQRPEPRD"
     gene            5783..>6156
                     /locus_tag="VLF19_12825"
     CDS             5783..>6156
                     /locus_tag="VLF19_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94185.1"
                     /translation="MSEWAHPAWPYLVGAALVGVLPRRALRPVLVVAPVAAVLVAWTL
                     PVEARVEWRLMGHPLLWMSVDRLARVFAVIFALIGGLGAVYAWSSDSRRLHVSAFTAA
                     AAAIGVSLAGDWVTLYLWWETLA"
BASE COUNT          658 a         2241 c         2126 g         1131 t
ORIGIN      
        1 tggcgcttct tgaattccga gaggagcccg gccttctcgc actgcttctt gaaccgtttc
       61 agtgcgctct cgaaggactc gtcgggctcg acgatgacct tggtcactcg actcccccct
      121 ctgcgatgcc gccgaccacc gtctgggtaa acgacgacta tataccaccc gccggcacca
      181 tccgctaccc ggagcgccca cgcgtgcgcg ccgctttcga gtcgaggccg tcggccgagg
      241 gacgagtaga atacccccga ctagaatacc cccggccccg gccggggggg agccatatga
      301 gagagcgtgc gggaggatgg tgaccgcttc gatcctgctc ttcgtggcct gcttcgcgct
      361 ctggctcgtc ctctcgggtc acttcgggct cctcgagatc gccttgggag tggcgtcggc
      421 ggcggccgtc acggcggcga accgcaatct cgaggcgctg agcccatttc tgagggtcgc
      481 gccgcggctg ctggcgtacg gggtgtggtt gctccgggag atcgtcgtgg ccaacctgca
      541 ggtgactcgc gtcgtgctgc atccccggat gccgatcgag ccggtcgtga gccgcttcga
      601 ggtgacgctg ccgggggacc tcgcgatcgc gaccctcgcc aactccatca cgctcacccc
      661 ggggaccatc accctcgaca tcgagggccg cgcgctcgtg gttcacgccc tcctcggccc
      721 ggccagcgtg ctcgcggccg agggtgagat ggcccggcgg gtgggccgcg tcttcgcggg
      781 acggatggcg tgactccgga gggcctgttc ctggccatga cgctcggctt gctggggctg
      841 gtcaccgtgt acctgtatcg cgtcgcggcg ggcccgacga tcttcgaccg gatcctcggg
      901 ctcagtggct tcggcaccaa gacgaccgtg gtgctgctgc tggtgggcgc gctgtatgcc
      961 cgcctcgaca tgttcgtgga cctggccttc gcctacgcgc tcctgagctt cgtcgggtcg
     1021 ctggccgcgg cccgctattt cgagcggggc ggtccgcatc ggtgacgatc gtcatcgcca
     1081 tcctcctggc cggggggttc ttcttccagg ccgtggcagc cctgggcgtc atccggctgc
     1141 cggacttcta cacgcgtctg cacgccgtga gcaaggcgga gacgctcggc gtgctgctca
     1201 cgctcgcggc gctcgtcgtg tggaccgggc cgagcctcgt cgcggtgaag gtgggcttcg
     1261 tggggctatt catgttcctt gccaacccca cgtcgaccca cgcgatcggt cgcgcggcct
     1321 tccggacagg tgtgaggccc tggctccgcg acccggagga gggccggtga gcgaggtcgt
     1381 cgtcgccgtc ctcctcgtgt tcctcggcgt gctcgccgtc gtcgcggtcc gggcggcgga
     1441 cgtgctcctc gcggccatcg ccctcggcgt ctacagcttc gtgatggcgc tgacgtgggc
     1501 ggcgctcggg gcggtcgacg tgtcgttcac ggagaccgtc gtcggtgccg gggcttccac
     1561 ggtgttcttc ctcgcggcgc tgcttcggac ctcgcgccgt cggtcgccgg cgccggggcg
     1621 gttccgctgg gcggcgctcg ccgtggtcgc cgcggtcggg gtgctgttga tcggcgcgac
     1681 gaccgacctg ccgcccttcg gcgacccggc tgcgccaccg tctgtccacg tctcgcctcg
     1741 ctacctcgag cgcacgcgag aagagacgcg cacgcccaac gccgtcaccg cggtcgtggc
     1801 cgattaccgc ggctacgaca cgctcatcga gaccgtggtg atcttcaccg gggggctggc
     1861 cggctcgctg ctgctggcgc gaccgcgatg atcgagcggc acgacagccc ggtcgtcacg
     1921 tcgttcgtgc gggtcctggt cccgatctcc cagctcttcg gcctctatgt cctggtgcac
     1981 ggccacgaga gtccgggggg cgggttccag gccggcgtga tcctggccgc cagctacatc
     2041 gtgctcgcgc tcggcctggg gcgtgaggcg ctggatcgcc gggtgaacga gccctggtgc
     2101 ctggcgctgg gcgcgctcgg ggtgctgatc tacgcgctca ccggagtcgt gggggtcgtg
     2161 cgggggcggg cattcctcga ctactccgcc ctgccgctcc ccgtctcgcc ggccgaggcc
     2221 cgttacttcg ggatcctgtt cgtcgagacc ggggtggcga tcgccgtcgc ggcgaccatc
     2281 gtggtcctgt tctgccggct ggcggacagg gagcacgcgc cgtgagcgcg ccgtactgga
     2341 tggccgttgc cctcctgatg ctcggactct acgtgatgat cgcccatcag aacctcttca
     2401 agaagttcgt gggcatgacg atcttccaga cggcgatcat cctgttcttc ctcctgctga
     2461 gcgtgaagcg gcacggcgac ctgcccatcg tgcccaacgg tccggtgtcg agcgagagct
     2521 tcatgaaccc gctgcctcac gcactcatgc tcaccgccat cgtcgtggcc gccgccacga
     2581 ccggcgtcgc gctcgccatc ctcatccggc ttcacgccgc ctacggatcg ctcgaggagg
     2641 acgtcgtcac ggggcacgcc gtgcgcgaat cctcgcccga ctcgtgaccg accacctggc
     2701 cgtcctcccc ctcgtcatcg tgctggtcgc ggcgctgctg cagcccatcg tcggactcgc
     2761 cgggcgccgg gccgcctggc cgctcgccgt cgccgccacc gccgtcgcca cgctcgccgc
     2821 gctggtcggc ctcggcgcgg tcctgacccg cggcacgctg cggtacgcgg tgggcgggtg
     2881 ggatccgccg tgggggatcg aggtcgtgct cgaccccttg agcggtttcg cggcttcggt
     2941 catcctcggc gtggcgacgc tgagcttgct cggcagtggc ccgttggtgc gcgcccgcca
     3001 ccggagcgag gagcccgtct actacgcgct cgccctgctg atgctcaccg ggctgaccgg
     3061 catcgtcatc agcggcgacc tgttcaacgt cttcgtgttc ctcgaggtcg cctcgatctc
     3121 cagctacgcc ctggtggcca gcggcggcgg tccggcactc gccgcagggt tccgctacct
     3181 cgtgctgggg acggtcggcg catccttcta cctgctcggt gtcggtctgc tctacgcgct
     3241 gactgggacg ctcaacatgg ccgatctggc ggcccggctc cccgccgtga ccggctcatc
     3301 ccccggggcc ggcgggatgg ccctcatcgt ggcgggcctg gcactcaaga tggggctgtt
     3361 cccgctccac ggctggctgc ccgacgccta cacgtactcc tctccgtcgg tcgcctcgtt
     3421 gctcggcccg gtggcgacga aggcggcggc ctacgcgctg gcccgcctgc tgctctacgt
     3481 cctggctgcc cgggagacgc cggtcgcgac gatcgtggcc tgggccgggg ccgcgtcgat
     3541 catggcgggc ggcgtcatgg cgctccgaca ggtcgatcca cgccgactgc tcgcctattc
     3601 gagcgtgagc cagatcgggt acatcgcgct gggaatcgga ctcgccaacg cgtcggctct
     3661 ctccggcgca tacctgcacg tgctcaacca cgccgtgttg aaggccacgc tcttcatcgc
     3721 cgtcggcgcc tcggccagcg atccgccggc cggaatgccc acgtggcgga agccgatcac
     3781 ggcggcctgc gcggtcgtcg ccgccctgtc gatggtcggc gtcccgccgg tcggcggatt
     3841 cttctcgaag tggtacctgc tcgagggggc gctggaggct cggcagccca tcttcgtcgc
     3901 cgtcatcctc gcgggtagcc tgctggccgt cggctacacg tatcgactca ccgaggccgt
     3961 gtggttcgag ggatcacgcg cggacggtcg ccgcgaagag gccccgccat ccgtcctgat
     4021 cgggctcgtc cttctcgccg tcctcgccgt ggccgccggg ctcgcgaact cggtgatcgt
     4081 cgacgcaatc ctcctgccgg cagtcgccgg agcgcggtag tggacgtcgc ctccacgctc
     4141 cccggctggg cgctggtgcc tccggcggcc gccacggcgc tgatcgccgc ctcccgtcgg
     4201 cgcccggtgg tcagggaagc ctggagcgtg gcggcggcca tcgcgcagtc cgcgatcgtc
     4261 gccgtcatgc tgccgcgggt gctggcggga gaacgcctcg tgtgggagcc ggtgtccctt
     4321 gcgcccgggg tgccgctgct ccttcgcgtc gatccgctcg gcgtgctgtt cgcgctcctg
     4381 gcctccgccc tctgggtcgt gacgaccgtc tactccatcg gctacacgcg ggccctgacc
     4441 gagccccgcc agacgcgtta cttcgcctcg ttcgctctca gcctgctcgc ggcggccggg
     4501 gtcgctatgg cgggcaacct cctcacgttc tttgtgttct tcgagatcct caccgtcgcg
     4561 acgtatccgc tcgtcgtcca ccgtggcaac gccgaggcgg cccgggctgg ccgcatctat
     4621 ctcgggtaca cgctgggggg cggcacggcg ctgctcggcg ccgtggcctg gacccaggtg
     4681 ctggccgggc ggctcgactt ccagccgggg ggcatgctcc acgccactgc tgcgagcccg
     4741 tcgcagctct gggggctgtt cgggctcttc gcgatcggcg tcggcgtcaa ggcggccctg
     4801 gtccccctgc acagctggct tccggtcgcc atgatcgcgc cgacgcccgt gagcgcgctc
     4861 ctccacgcgg tggccgtggt caaggccggt gtcttcggca tcgtacgggt tgtcggcttc
     4921 gtgttcggca tcgatctgct gcaggacctc ggagcggctc ccgtgctggc cggcatcgcc
     4981 ggcgcgacga tcgtgctcgg gtcgctcgcc gcgctcgccc aggacaacct caagcgtctc
     5041 ctggcgttct ccacggtcag tcaactgtcc tacatcggcc tcggcgcgtc cctcggcacc
     5101 ccggacgcca tggccggcgc cgtgatgcac atcgccagcc acgctctcct gaagatcacg
     5161 ctgttcttct gcgccggcgc gatctacgcg acgctgcacc tggaacgggt gagcgagctg
     5221 gatggcgtgg gacgccggat gccgctgacg atgggcgcgt tcgcgacggc agcgttcatg
     5281 ctggccggcc tgccgccggg acccgcgttc gtcagcaagt ggtatctcct gaccggcgcc
     5341 gtggcggcca gcgcctggtg ggccgtcggc gtgctgctgg ccagcacgct cctgaatctc
     5401 gcctatttca cgcccatcgt cgtcagggca ttctggggcc gaggggcggg cccgtcacag
     5461 gaggcgcggc cggccctgct gatcccgctg ctggccacgg ccggcgcagc gattgtcctc
     5521 ggcctggccc cggaggcgcc cggcgggctg ctggcgctgg cgcgcgacgt cgccaccggc
     5581 acgatcgccg cccgatgatg agggtcctgg cggcgctgag cggtctcttg atcctgctcg
     5641 tcgcgctcga gatcgcgctc gtgcccgagc ctcaccgcgt gcgaggggcc ttcgccgtca
     5701 tcggcctcgt gagctgcgtg acgatcgtcc tcgtgtccaa gctcctcggc aaagtctggt
     5761 tgcagcgtcc ggagccccgc gattgagtga gtgggcgcac ccggcctggc cgtacctcgt
     5821 cggcgccgcg ctcgtcggcg tccttccccg ccgggccctc cgcccggtcc tcgtcgtcgc
     5881 gccggttgcg gcggtcctcg tcgcctggac gcttccggtc gaagcccggg tcgagtggcg
     5941 gctcatggga caccccctcc tctggatgag cgtcgaccgg ctggcgcggg tcttcgccgt
     6001 gatcttcgcc ttgatcggag gcctcggcgc cgtctacgcc tggtcctccg actcgcggcg
     6061 cttgcacgtc tccgcgttca ccgcggccgc cgccgccatc ggtgtcagcc tcgccggcga
     6121 ctgggtcacg ctgtacctct ggtgggagac cctggc
//
LOCUS       DATIAZ010000374         5976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1572999, whole genome shotgun sequence.
ACCESSION   DATIAZ010000374 DATIAZ010000000
VERSION     DATIAZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572999"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VLF19_12830"
     CDS             complement(<1..563)
                     /locus_tag="VLF19_12830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HSE94186.1"
                     /translation="MRRFTVAPERVDGDRVTFDREETRHMRSVLRLAPGDVVVAGDGR
                     GRDYTVRIEALDEIASGTVVGVAASSAEPPLAITLIQGVPKRDKMELIVRAATELGVA
                     RVCPALTERTIVRLEPGRWRERARRWQRVAKEAAKQSGRAVVPDVELPRPIAEWLAEA
                     RQELRICLWEGQAPALAVVLAAVARPPT"
     gene            complement(570..1448)
                     /locus_tag="VLF19_12835"
     CDS             complement(570..1448)
                     /locus_tag="VLF19_12835"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSE94187.1"
                     /translation="MTAAYWELAVAVPPDAAEPVTNFLWELGALGVVEEEMPGASPRL
                     RAFFPAHADAPQLATRLSAYGEGLGALGLPSPGPPSVAPLADADWAAAWRDHFRPLPV
                     GRRLVIAPPWDAPALAGRLTVVIEPGRAFGTGHHGTTAGCLEALEALVEQGVPAHAID
                     LGTGSGILAVAAACLGVERVVAVDEDPDAVAAAIANAARNGVTDRVRCAVADVGGLDT
                     AAAPLVLANLLSAAHARLAPRYAEYVAPGGALVLGGILDHEAAGVARALARHGFVSRA
                     TLSLDGWTTLHLARGH"
     gene            complement(1473..2573)
                     /gene="dnaJ"
                     /locus_tag="VLF19_12840"
     CDS             complement(1473..2573)
                     /gene="dnaJ"
                     /locus_tag="VLF19_12840"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSE94188.1"
                     /translation="MARDYYEILGVPREATDTDIKKAYRQLAMQHHPDRNPGDKQAEE
                     RFKEASEAYAVLSDPDKRAQYDRFGTVAAGGGFSGDFGQGFGTLFEDIFENFFSGGGG
                     RGRRSRVARGDDLQYELKITLEEAANGLETKVQIPRMEPCEACHGDGVEPGARRATCD
                     ACGGRGEIRLSQGLLTVARTCPRCHGEGEVNRNPCTACRGEGRQRAERMLSIKIPAGI
                     DDGMQLRLSGEGSAGWHGGPPGDLYVLVRLREHALFARNGADLHCDLPVSFAQLALGA
                     EIEVPVLGGTAKLKVPAGSQPHQVLRLRGRGMPRLRERGQGDTCYRLILEVPQKLNGR
                     QREALEAYEAASKGQSGPLGASFLERMKKLLE"
     gene            complement(2577..3092)
                     /locus_tag="VLF19_12845"
     CDS             complement(2577..3092)
                     /locus_tag="VLF19_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSE94189.1"
                     /translation="MTEPGAETPASDGSPESVLDDVRRQLEEKQDRLLRTLAEMENMR
                     RRGQREREDYVRYANESLLRDLVPVLDNFDRALAAARATGEAPKVVEGVELIQRELSR
                     MLERHGVTRYSALGQPFDPTRHEATASVVSADQPANTVVGEIAPGYLLNGRVLRAAQV
                     AVAVAPDQDAA"
     gene            complement(3165..4187)
                     /gene="hrcA"
                     /locus_tag="VLF19_12850"
     CDS             complement(3165..4187)
                     /gene="hrcA"
                     /locus_tag="VLF19_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HSE94190.1"
                     /translation="MDERHRGVLVALIREYVDSAQPVGSRLLAKRHFRRLSPATIRNV
                     MADLEEMGYLAQPHTSAGRVPTDKAYRFYVDSFPRPSGTDERAPETRPARPPAIDGFM
                     ERTSEHLSTVTKMTGLLLAPPLRHTTIGRVELMPLEDDRALAVIVTDTGWVTARAITL
                     DPPLAADEVRKIGRELTRRFRDRTVAEIVEMEAAPADPLDELHTRARAITEQILALLR
                     GRTLYVSGAMNMLDHPEFWNLETTRDLLRTFEQKERLADLMASLAQGEGMQVTIGREN
                     PYAEMRECTLITSTYLYRNQVLGILGVVGPRRLPYPDVISVVNETARHVTEALSRVRQ
                     DLYLPS"
     gene            complement(4412..5509)
                     /gene="hemW"
                     /locus_tag="VLF19_12855"
     CDS             complement(4412..5509)
                     /gene="hemW"
                     /locus_tag="VLF19_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSE94191.1"
                     /translation="MPFCEQRCHYCSFNTAPRDDEGLVRYLDAVNREVRLLAALPWAG
                     AVTIGTVFLGGGTPSLLAPDDLAHLLHGLRAGLALAPGAEVTVECNPESVTRAKLERY
                     RAAGVNRLSLGVQSLDDVLLERLGRLHDGRRAREAYVAAREAGFDNVSVDLMYGLPDQ
                     DVAIWSRTVDAVLAWSPDHVSAYGLTLDGGSAWGAGGVAGLPAEDTVVEQYWALTRAA
                     AARGLEHYEISNYARPGFHARHNEIYWQAGEYLAAGPGGCGHVGGVRYGTARSLPRYC
                     AALEGGALSIDSAERLTPRQRLGERLMLGLRTADGVPAAWFRERLDADPALTRRVDAW
                     REHGLLVTREDRVALSEAGFLLSDALFVELL"
     gene            complement(5839..>5976)
                     /locus_tag="VLF19_12860"
     CDS             complement(5839..>5976)
                     /locus_tag="VLF19_12860"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HSE94192.1"
                     /translation="DNSQDSRYWGFVKRDKIKGKAFLIYWSWDSDRHWLRWWRLGNYI
                     P"
BASE COUNT          849 a         2135 c         2173 g          819 t
ORIGIN      
        1 gtcgggggcc tggcgaccgc cgcgaggacg acggccaagg cgggcgcctg gccttcccac
       61 agacagatcc tgagctcttg gcgcgcttcg gcgagccact cggcgatcgg ccggggaagc
      121 tcgacgtcgg ggaccacggc gcggcccgac tgcttggcgg cttccttggc cacccgctgc
      181 caacgccgcg cccgctcgcg ccagcggccg ggctcgagcc tcacgatggt gcgctcggtg
      241 agcgcggggc agacgcgcgc gacgccgagt tccgtggcgg cgcgcacgat gagctccatc
      301 ttgtcgcgct tgggcacgcc ctggatcagc gtgatcgcga gcggaggctc ggcgctgctc
      361 gcggccacgc cgacgacggt gccgctcgcg atctcgtcga gcgcctcgat gcggacggtg
      421 tagtcgcggc cgcgcccgtc gccggccacc acgacatcgc ccggagccag gcgcagcacc
      481 gagcgcatgt ggcgcgtctc ttcccgatcg aacgtcacgc ggtcgccgtc cacgcgttcg
      541 ggggccacgg tgaaccggcg cacggatcgt cagtgtccgc gtgcgaggtg gagcgtcgtc
      601 cagccgtcga gcgagagcgt cgcacgcgac acgaagccgt gacgggcgag cgccctggcc
      661 acgccggccg cctcgtgatc gaggatgccg cccagcacca gtgcgccacc gggcgcgacg
      721 tactcggcgt agcgcggtgc gaggcgcgcg tgggccgccg acaacagatt cgcgagcacg
      781 agaggcgccg ccgcagtgtc gaggccgccc acgtcggcca ccgcgcagcg cacccggtcg
      841 gtcacgccgt tgcgcgcggc gttggcaatc gcggccgcca cggcgtcagg gtcttcgtcc
      901 accgccacca cgcgctcgac ccccaggcag gcggcggcga cggccagaat gccggagccc
      961 gtgccgaggt cgatcgcgtg ggcggggacg ccctgctcga cgagcgcctc gagggcctcg
     1021 agacagcccg cggtcgtgcc gtgatggccg gtgccgaagg cgcggccggg ctcgatcacg
     1081 acggtgaggc gtcccgcgag agcgggcgcg tcccacggtg gggcgatcac gagccgacgg
     1141 ccgacgggca ggggccggaa gtggtcccgc cacgccgccg cccagtcggc gtccgcgagc
     1201 ggcgccacgg agggcgggcc tggcgacggc aggccgagcg cgccgagccc ttcgccgtac
     1261 gcgctcaggc gagtcgcgag ttgcggcgcg tcggcgtgag cgggaaagaa ggcgcggagc
     1321 ctcggggagg caccgggcat ctcctcctcg acgacgccga gcgcgccgag ctcccagaga
     1381 aagttggtga ccggctcggc agcgtccggg gggacggcga cggcgagctc ccagtaggcg
     1441 gcggtcatca gcgctctgcg ggcgccggac ggctactcga gcagcttctt catgcgctcg
     1501 agaaacgacg ctccgagcgg cccgctctgg cccttggagg cggcctcgta ggcttcgagg
     1561 gcctcgcgct gccggccatt gagcttctgg ggcacctcga ggatcaggcg gtagcaggtg
     1621 tcgccctggc cgcgctcgcg caggcgcggc atgccccggc cgcgcagtct caggacctga
     1681 tggggctggc tgccggccgg caccttgagc ttcgccgtgc cgccgagcac gggcacttca
     1741 atctcggcgc cgagggcgag ctgcgcgaac gacacgggca ggtcacagtg gagatcggcg
     1801 ccgttgcgag cgaacagcgc gtgctcgcgg aggcggacca gcacgtacag gtcaccgggc
     1861 ggcccgccgt gccagcccgc ggagccctcg ccggaaaggc gaagctgcat gccgtcgtcg
     1921 atccccgccg ggatcttgat cgagagcatg cgctctgcgc gctgacgccc ctcgccccgg
     1981 cacgccgtgc agggattgcg gttcacctcg ccctcgccgt gacacctcgg acacgtgcgc
     2041 gccaccgtga ggaggccctg cgacagccgg atctcgccgc gcccgccgca cgcgtcgcac
     2101 gtcgcgcgcc gtgcgccggg ctcgacgccg tcgccgtgac acgcctcgca cggctccatg
     2161 cgcgggatct ggaccttggt ctcgagaccg ttcgccgcct cctcgagcgt gatcttcagc
     2221 tcgtactgga ggtcgtcgcc gcgcgccacg cgcgagcggc gcccgcgccc gcccccaccc
     2281 gagaagaagt tctcgaagat gtcctcgaag agcgtgccga agccctggcc gaagtcgccg
     2341 ctgaagcccc cgcccgcggc gacggtgccg aaacggtcgt actgggcacg cttgtcggga
     2401 tcggacagca ccgcgtaggc ctcgctggct tccttgaagc gctcctcggc ctgcttgtct
     2461 ccgggattgc ggtccggatg gtgctgcatg gcgagctggc ggtaggcctt cttgatgtcg
     2521 gtgtcggtgg cctcgcgcgg gacgccgagg atctcgtagt agtcgcgcgc catggactag
     2581 gcggcgtcct ggtcgggcgc gacggcgacg gcgacctgcg cggcgcgcag gacccgcccg
     2641 ttcagcaggt agccgggcgc gatctcgccg accacggtgt tggcgggttg gtcggcgctc
     2701 accacgctgg cggtggcctc gtgacgcgtg gggtcgaagg gctgaccgag ggcggagtag
     2761 cgcgtcaccc cgtgacgctc gagcatcctg gagagctccc gctggatcag ctcgaccccc
     2821 tccacgacct ttggagcttc cccggtcgcc cgcgccgcag ccagcgcgcg gtcgaagttg
     2881 tcgagcacgg gcaccaggtc gcggaggagc gattcgttgg cgtagcggac gtaatcctcg
     2941 cgctcccgct ggccgcggcg ccgcatgttc tccatctcgg cgagcgtgcg caggagccgg
     3001 tcctgcttct cctccagctg gcgtcggacg tcgtccagga cgctctcggg gctgccgtcg
     3061 gacgccgggg tctcggcgcc cggttcggtc acgcgcgaat ccggtcgatc aaggtcactc
     3121 atggcacgag aaattatagc agcgccccac cggggcggcc gcgctcagga gggcaggtag
     3181 aggtcctgcc gcacgcgcga cagggcctcg gtgacgtgac gcgcggtctc gttgacgacc
     3241 gagatgacgt cgggataggg caggcgccgc gggccgacca cgcccaggat gccgagcacc
     3301 tggttccggt acaggtacgt ggaggtgatc agcgtgcact cccgcatctc cgcgtacggg
     3361 ttctcacggc cgatggtgac ctgcatgccc tcgccctggg caaggctggc catgaggtcg
     3421 gcgagccgct ccttctgctc gaacgtacgc agcaggtcac gcgtggtctc gaggttccag
     3481 aattccgggt gatcgagcat gttcatggcg ccgctcacat acagcgtgcg gccgcgcagg
     3541 agcgcgagga tctgctcggt gatggcgcgt gcgcgcgtgt gcagctcgtc gagcgggtcg
     3601 gccggggcgg cctccatctc gacgatctcg gcgaccgtac ggtcgcggaa ccgccgtgtg
     3661 agctcgcgtc cgatcttccg cacctcgtcg gcggccagcg gcgggtcgag cgtgatcgcg
     3721 cgcgccgtca cccacccggt gtcggtcacg atcacggcga gggcgcggtc gtcctccagc
     3781 ggcatcagct ccacgcggcc gatggtcgtg tgtctgagcg ggggggccag gagcaggccc
     3841 gtcatcttgg tcacggtgga gagatgctcg gacgtccgct ccatgaagcc gtcgatcgcg
     3901 ggcgggcggg ccggtcgcgt ctccggcgcc cgttcgtcgg tcccggacgg ccgcgggaag
     3961 gagtcgacgt agaagcgata ggccttgtcg gtcggcacgc ggcccgcgct ggtatggggc
     4021 tgcgcgaggt atcccatctc ctccaggtcg gccatgacgt tgcggatcgt ggccggcgac
     4081 agccgtctga agtggcgctt ggccagcagg cgagagccga ccggctgcgc cgagtccacg
     4141 tactcgcgga tcagggcgac gaggacgccg cggtgccgct catccatcta aaatcttcta
     4201 acttttgcct gggcccaagt caagccacgc cgaccgtgcc gtcggccgaa gcgcgcgctc
     4261 gacaccgtcg agcttcctgc gagatggtct ggctcgccgg tcctcccgaa cggagcgggt
     4321 gggaagtggc cgcgactcga gcgccggcgc cgccggcgcc aaccggttcc gggaggtctc
     4381 ggccggggcg gtcgaggccc ccgccgacga gctacagaag ctcgacgaag agggcatcgg
     4441 agagcaggaa gccggcctcg ctgagcgcga cgcggtcctc cctcgtcacg agcaggccgt
     4501 gctcgcgcca ggcgtcgacg cgacgggtga gggccggatc ggcgtcgagg cgctcgcgga
     4561 accacgcggc cggcacgccg tcggcggtgc gcaggcccag catcagccgc tcgccgagtc
     4621 gctggcgcgg tgtcaggcgc tcggcggagt cgatcgacag ggcgccgcct tccagcgcgg
     4681 cgcagtagcg cggcagcgac cgggcggtgc cgtagcggac accgcccacg tggccgcacc
     4741 cgccgggccc ggcggccagg tattcccccg cctgccagta gatctcgttg tgtcgcgcat
     4801 ggaagcccgg ccgcgcgtag ttcgagatct cgtagtgctc gaggccgcgc gccgcggcgg
     4861 cgcgcgtcag tgcccagtac tgttcgacca cggtgtcctc ggcgggcagc ccggcgacgc
     4921 cgcccgcgcc ccacgcgctc ccgccgtcga gggtgaggcc gtaggcggac acgtggtcgg
     4981 gcgaccacgc gagcacggca tcgaccgtcc ggctccagat ggcgacgtcc tgatcgggca
     5041 ggccgtacat gagatcgacg ctcacgttgt cgaagccggc ctcgcgggcg gcgacgtagg
     5101 cctcgcgcgc gcggcggccg tcgtgcaggc gacccaggcg ctcgagcagc acgtcgtcga
     5161 gcgactgcac gccgaggctc agtcggttca cgcccgccgc acggtagcgc tcgagcttcg
     5221 cgcgcgtcac actctccgga ttgcactcga ccgtgacctc ggcccccgga gccagggcca
     5281 ggccagcgcg cagcccgtgg aggagatgag cgagatcgtc gggcgccaag agcgacggcg
     5341 tgcccccccc gagaaacacc gtgccgatcg tcaccgcccc agcccacggc agcgccgcca
     5401 gcaaccgcac ctcgcggttg acggcgtcga gatatcgtac gagcccctcg tcgtcccgcg
     5461 gggccgtgtt gaaggaacag taatgacagc gctgctcaca gaacggcatg tggatgtaca
     5521 acccgagcga ccgaccgtcc ggggccgtgt ccgtcaacgg tccgaacggc gacggccagg
     5581 cattcgcatc ggggtcgagg aggcggcgcg ccgtcgggga ggccccggcc agcccccctt
     5641 ccctggcagg ccgcatcatg gggacaccat caatcagatc cgatcgacgc ccgcggcccc
     5701 gaccggcggg tacggccacc cacggagcga accggccgcg ccgccgccgc tacgatgaac
     5761 acgagcggta acaccacccg cgctccatcg acctcaccaa ggaacacgag cgagcccacc
     5821 acggccgctc gatcaacctc acgggatata gtttcccagc cgccaccagc gaagccagtg
     5881 acggtcgctg tcccacgacc agtagatcag gaaggccttg cccttgatct tgtcccgctt
     5941 cacgaacccc cagtaccggc tgtcctggga gttgtc
//
LOCUS       DATIAZ010000375         3538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1706421, whole genome shotgun sequence.
ACCESSION   DATIAZ010000375 DATIAZ010000000
VERSION     DATIAZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706421"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VLF19_12865"
     CDS             <1..195
                     /locus_tag="VLF19_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94193.1"
                     /translation="EPAPDRPFALVSDGVVVGGIPMPGPLVDWVMRHYDPSRALAARA
                     PFDIAIAPVHIAPDAVRIGE"
     gene            406..1098
                     /gene="nth"
                     /locus_tag="VLF19_12870"
     CDS             406..1098
                     /gene="nth"
                     /locus_tag="VLF19_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSE94194.1"
                     /translation="MAESREAKKARARKILGALERAYPDAKIALEFDSPLELLVATIL
                     SAQCTDERVNMVTRSLFEKYRRAEDYAAADPATFEAEIRSTGFFRSKARSVVGMAQAL
                     VARHGGRVPRTREALRALPGVGLKTANVVLGNAFGQPAIAVDTHVFRVTQRLGLARGD
                     DPDRIHDQLSEVLPRAKWTRACHLVQAHGRRTCVARTPLCPVCPVRALCPWPGKTRPP
                     ARASSRAGAAVR"
     gene            1194..1622
                     /gene="rplM"
                     /locus_tag="VLF19_12875"
     CDS             1194..1622
                     /gene="rplM"
                     /locus_tag="VLF19_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSE94195.1"
                     /translation="MPKETEIERKWFVVNAEGQVLGRLATRVATVLRGKHKPTFTPHL
                     DVGDHVVVVNAEKVHLTGRKLRDKMYRWHTGYIGGLREVNAEKMLRTHPERVIEWAVQ
                     GMLPKTRLGRAMAKKLKVYRGAEHPHQAQRPEPLELREAR"
     gene            1622..2017
                     /gene="rpsI"
                     /locus_tag="VLF19_12880"
     CDS             1622..2017
                     /gene="rpsI"
                     /locus_tag="VLF19_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSE94196.1"
                     /translation="MAVLDRAYGTGRRKTSVARVWVRPGSGRIVINRRSFEDYFPRET
                     LRMIIAQPLQITNVAGQIDIVCTVGGGGPTGQAGAVRHGISRALVRFDENFRQPLKKA
                     GLLTRDPRMRERKKYGQPGARQKFQYSKR"
     gene            2022..3083
                     /gene="argC"
                     /locus_tag="VLF19_12885"
     CDS             2022..3083
                     /gene="argC"
                     /locus_tag="VLF19_12885"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSE94197.1"
                     /translation="MGGKKIRVAVAGASGYMGAEALRLLLRHPMVELTGVTSDRLAGD
                     PLARAFPHLRGLTDLAFHDLDAEWLASVADAVFLALPHMESQKVVPVLRERRVKAIDL
                     SADYRLRNANDYTVWYKAPHVDPTGLAEAVYGLPELHRKAIAGASLVAAPGCYPAGAI
                     LATAPLLKGGLARLEGIVIDGKSGVSGAGAQGRKADPMYLYTEANENVQAYALASHRH
                     TPEIEQELSALAGAPLRVSFTPHLVPLNRGLFTTASVPLAVSTTTAELTALYRDFYAD
                     EPFVRVLAEGERPATRMVVGSNFCDVAVVADPRTSRAVCVSALDNLGKGGSANGVQNL
                     NILFGWDERTGLDAPPVYP"
     gene            3155..>3538
                     /locus_tag="VLF19_12890"
     CDS             3155..>3538
                     /locus_tag="VLF19_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HSE94198.1"
                     /translation="MTAVPGILAGGLAAGIKPSGKKDLALVYSSAPARAAALFTTNQV
                     KGAPVIVSMEHVRGGVAQAIVASSGCANVCTGEQGLRDAREIARITGELLRVPARHVL
                     VASTGVIGVPLPMDRIRAALPKLAKA"
BASE COUNT          519 a         1243 c         1274 g          502 t
ORIGIN      
        1 gagcccgcgc cggaccgtcc cttcgccttg gtgagcgacg gggtcgtcgt gggcggcatc
       61 ccgatgccgg ggccgctcgt agactgggtc atgcgtcatt acgatccctc gcgcgccctg
      121 gccgcccgcg cgcccttcga catcgcgatc gctcccgtcc acatcgcccc cgacgccgtt
      181 cgcatcggcg agtgacgtct ctcgtcggtt gctcgcgaac cgtgggccgg gcggggagtc
      241 cgcccgggac cgtgtcttct gcggatagac gagaactctg acagcatcgg cgcgggtcag
      301 ggcctgcgca cgcgcacggt ggatagcgcg tcccggggcg ggcgacccag gccccaaccg
      361 gcgcctcggc aggcgagggc ggactcggga aaccaggagg cggagatggc agagtcgcgg
      421 gaggctaaga aggcgcgggc ccggaagatc ctcggcgcgc tcgagcgcgc gtacccggac
      481 gcgaagatcg cgctggagtt cgacagcccg ctcgagctcc tggtggcgac gatcctctcc
      541 gcgcaatgca ccgacgagcg ggtcaacatg gtcacgcgct cgctcttcga gaagtaccgg
      601 agggccgagg attacgccgc cgccgacccg gccaccttcg aggccgagat ccgttcgacc
      661 ggattctttc gatcgaaggc cagatccgtg gtcggcatgg cccaggcgct ggtggcccgg
      721 cacggcggcc gggtgccgcg cacgcgcgaa gcgctgcggg cgctgccggg ggtcgggctc
      781 aagaccgcca acgtcgtgct cggaaacgcc ttcggccagc cggcgatcgc cgtggacacg
      841 cacgtcttcc gcgtgacgca gcgcctgggg ctcgcgcgcg gcgacgaccc cgaccgtata
      901 cacgaccagc tctccgaggt gctcccacgc gcgaagtgga cgcgcgcctg ccacctggtc
      961 caagcccacg gccggcgcac gtgcgtggcc cggaccccgc tctgcccggt ctgtcccgtg
     1021 cgcgcgctct gcccgtggcc gggcaagacc cgccccccgg cacgggcgtc atcgcgggcc
     1081 ggcgccgcgg ttcgttgaca ggggtgggct tttcaactac acttggccgt tgcaaatttc
     1141 tccgtatcca ggcgcactta gagagtcctg aaggggtaac gatgccgacg ccgatgccga
     1201 aggagaccga aatcgaacgg aagtggttcg tcgtgaacgc ggaggggcag gtgctgggac
     1261 gactggcgac ccgcgtggcg acggtcctcc ggggcaagca caagccgacc ttcacgcccc
     1321 acctcgacgt cggcgatcat gtcgtcgtcg tcaatgccga gaaggtccac ctcaccggcc
     1381 gcaagctgcg cgacaagatg taccgctggc acaccggcta catcggcggg ctgcgcgagg
     1441 tgaacgccga gaagatgctc cgcacgcatc ccgagcgcgt catcgaatgg gcggtgcagg
     1501 gaatgctgcc gaagacccgt ctggggcgcg cgatggccaa gaagctcaag gtctaccgcg
     1561 gcgccgaaca cccgcaccag gcccagcggc ccgagccgct ggagctccgg gaggcccgtt
     1621 gatggccgtg ctcgaccgcg cctacggtac gggacgtcgg aagacctcgg tggcgcgcgt
     1681 ctgggtccgg cccggctccg gccggatcgt gatcaaccgc cggtccttcg aggactattt
     1741 tccgcgcgag acactgcgga tgatcatcgc gcagccgctg cagatcacca acgtcgccgg
     1801 ccagatcgac atcgtctgca cggtcggcgg cggtggcccc accgggcagg cgggcgccgt
     1861 gcgtcacggc atctcgcgcg cgctcgtgag gttcgacgag aatttccgcc agcccctcaa
     1921 gaaggcgggc ctcctcaccc gcgatccgcg catgcgggag cgcaagaagt acgggcagcc
     1981 gggcgcgcgc cagaagttcc agtactccaa gcggtagcgc ggtgggcggg aagaagatcc
     2041 gggtcgccgt cgccggcgcc agcggctaca tgggcgcgga ggcgctgcgt ctgctgctcc
     2101 gccatcccat ggtcgagctg acgggcgtca cctccgaccg cctggccggt gatccgctgg
     2161 cccgggcgtt cccacacctg cgtgggctga cggatctcgc gttccacgat ctcgacgccg
     2221 agtggctggc gtccgtcgcg gatgccgtgt tcctcgcgct gccccacatg gagtcccaga
     2281 aggtcgtccc ggtgctccgc gagcggcgcg tgaaggcgat cgacctgtcg gcggactatc
     2341 ggctgcggaa tgccaacgac tacaccgtct ggtacaaggc gccgcacgtg gacccgaccg
     2401 gtctggcgga ggccgtctac gggctgccgg agctgcaccg caaggcgatc gccggcgcgt
     2461 cgctggtggc agcaccgggc tgctacccgg ccggcgcgat cctcgcgacg gcgccgctgc
     2521 tcaagggcgg gctggcgcgg ctcgagggca tcgtgatcga cggcaagtcc ggcgtgagcg
     2581 gagcgggagc gcagggccgg aaggccgatc cgatgtacct ctacaccgaa gccaacgaga
     2641 acgtgcaggc gtacgcgctg gcgagccatc gccacacgcc ggagatcgag caggagctgt
     2701 cggcgctcgc gggggcgccg ctgcgcgtgt cgttcacccc gcacctggtg ccgctcaacc
     2761 gggggctctt caccaccgcg tcggtgccgc tggccgtgtc gacgacgacg gccgagctga
     2821 ccgcgctcta ccgcgacttc tacgcggacg agccgttcgt ccgcgtgctc gccgagggcg
     2881 agcggccggc gacgcgcatg gtcgtcggct cgaacttctg cgacgtcgcc gtggtggccg
     2941 acccgcgcac gtcgcgcgcg gtgtgcgtgt cggcgctgga caacctcggc aagggcggct
     3001 ccgccaacgg tgtgcagaac ctcaacatcc tgttcggctg ggacgagcgc acgggtctcg
     3061 acgcgcctcc ggtgtatccg tagtgccggg cacgcgcgtg tccccgagcc gggcggtttc
     3121 ccgtccgtcg gacgtcgagt ggctggaagg cggcgtgacc gcggtgcccg gcatcctggc
     3181 cggcggcctc gccgccggca tcaagccgag tggcaagaag gatctggccc tggtgtactc
     3241 gtcggcgccc gcccgggcgg cggcgctctt cacgaccaac caggtcaagg gcgcgccggt
     3301 gatcgtatcg atggagcacg tgcgcggcgg cgtggcccag gccatcgtgg cctccagcgg
     3361 ttgcgcgaac gtgtgcacgg gcgagcaggg cctgcgcgac gcgcgtgaga tcgcgaggat
     3421 caccggcgag ctcctgcgcg tccccgcccg gcacgtgctg gtcgcgtcca ccggcgtgat
     3481 cggcgtgccg ctcccgatgg acaggattcg cgccgcgctc cccaagctcg cgaaggcc
//
LOCUS       DATIAZ010000376        17454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1310862, whole genome shotgun sequence.
ACCESSION   DATIAZ010000376 DATIAZ010000000
VERSION     DATIAZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 17454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17454
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310862"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1377)
                     /locus_tag="VLF19_12895"
     CDS             complement(289..1377)
                     /locus_tag="VLF19_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE94199.1"
                     /translation="MPGVSVRGLTKRYGDLAAVESLSLDVKPRELVALLGPSGCGKTT
                     TLRLVAGFLTPEAGEIWVGDRCLSSPSSVVPPERRRMAMIFQSYALWPHLTVAQNVGY
                     GLRFKRDLSRAERATRVKEILQVVQLAGFEDRYPGELSGGQQQRVAVARSLVVEPEIL
                     LLDEPLSNLDANLREEMRFEIRRLHERFGITTLYVTHDQGEAMVISDRIAVLQRGRVA
                     QIGTAEELFQQPRTRFVADFIGKTNLVDAVAGGRDVVERGDLRLRVAAADLEPGRRVA
                     VSIRPHEIAIAAGDRPSEVVASRNNLLRGTVQRTAYLGDALHYQVELERGDLTLRVVG
                     LPAMRIQAGAAVTLAIAPEACVVLADDD"
     gene            complement(1481..3157)
                     /locus_tag="VLF19_12900"
     CDS             complement(1481..3157)
                     /locus_tag="VLF19_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSE94200.1"
                     /translation="MRAIAMDRSVAVWVLAAVALVVMMALPLGWLGWLSVSSERGPTL
                     SHYARVFADGHLQKALWNTVVLALSVGLASLVVGAPMAWLTARTDLPGKGLIRGLIMA
                     SFVTPPFLGAFAWVMLAGPNAGLLNKAWRAATGAEDALIDIFTMPGLVFVVAIYTFPY
                     VYIMITNTLGLISSDLEEAASILGASRLRVAATVTLPMVAPAILSGFILAVLQALALF
                     GSPAILALPAGFHTITTQIWSFFHYPPKAEMAAAFSIPLLLATALLLQAQKRLLGRKG
                     YAAIGGKGGQRRAIRLGTWRWPALAACVAVMACSIFLPYGILTKAALARAWAQPLGWD
                     NLTLSNITFTFFEYGATKSAILNTLELGILTATVGAGLAALLAYVANRRLVYGHQVLA
                     FLALAPVVIPGVVLAVGLFIAYTRPPFLLYGTIWILFIAYVTKEMPVGYSQADATFKG
                     VHVELEEAGRVLGAGRLRVLREITAPLAKSGIIATWCFIFIGVIRELSASIILFTPDT
                     KVMSVVIFDLKEEGQFGAIAVLGLFMLLMTFTTVVLMQRVLGRDFMGVRE"
     gene            complement(3168..4196)
                     /locus_tag="VLF19_12905"
     CDS             complement(3168..4196)
                     /locus_tag="VLF19_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSE94201.1"
                     /translation="MDRREFVTLTVTGALALTASIPAARAQDARVEAAKKEGKVVWYT
                     SLVLPSAEKVAKMFEAAHPGIKVEVHRTGSQRVLQRLMQELQANIRNADVVHTSDAGH
                     FVLLKDKKLLMKYTPPGVDSFPVGFKDRDGYHFGLRATVNAIAYNTTLVPAAEAPKTW
                     KDLLDPKWKGKLVSAHPGYSGVIATHVLALVHLHGWDYFKQLAQNRVMLVQSAADPPG
                     VVAAGERQVAVNGGDYSAYQAKKKGAPIELVYPKEGVPLVVSPTAITAFAPHPNAAKL
                     FTDFTFTRELQQVMADSEGLYTGHPRVTYPADKPRLTELKLLQVDPDELEKRNEEIKK
                     RFVEFFGA"
     gene            complement(4208..5587)
                     /locus_tag="VLF19_12910"
     CDS             complement(4208..5587)
                     /locus_tag="VLF19_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HSE94202.1"
                     /translation="MGDVSTARRLARFVLGLDLASVPASVVERARRLTLDILGCALAA
                     SSMDFARAALATARGLGGPAESTLIGGGARVGAANAVLANATLAHGLDFDDTREDAIV
                     HTGCVAVPVALALAQATAASGAAVLTALVAAVEVMCRVGLAVPGRLHARHFHPTAVTG
                     VFGAAAAAGRLYRLDEDRLVNAFGICGSQASGIIEYLTDGTWTKRLHPGWAAHAGVVA
                     ALLARDGFTGPEHVLEGRHGFYAAFADGHNEVTLEALLGTLGREWEIERLTFKPFPCG
                     SIAQPYMDCALRLRSEHAIAPDDVVEIRCRTAAGPVPRLWEPLAAKHRPPNGYAAKFS
                     LPYLIAVILIRGRASLAEFTDEAVRDEAVLRLAGRVSYDLDETIDYPRQFIGQVSVRL
                     RDGRVLDARQNHPRGGADHPLTAAELEAKFRGNAGLVLPAARIDRIIAEVGGLGQALP
                     LDDLMEALQ"
     gene            complement(5598..6383)
                     /locus_tag="VLF19_12915"
     CDS             complement(5598..6383)
                     /locus_tag="VLF19_12915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSE94203.1"
                     /translation="MASLERRVAIDAARDAGDLLRREVRGPRRITYKGAPTNLVTEMD
                     ARAEALILQRLVAAFPDDGVLSEEAGAQPGRSGRRWIVDPLDGTTNYAHGVPVFAVSI
                     ALEAAGRAVLGVVYDPNLEELFVAERGGGAFLREERLAVSATSVLDESLLTTGFPYSI
                     RQIADNNLREYAAFSVRARAVRRLGSAVIDFAWVAAGRYDGFWELTLGAWDAAAGALL
                     VEEAGGRVTDLGGGPLDLDRPAIVATNGRIHDEVLAVLKEIRG"
     gene            6518..7477
                     /locus_tag="VLF19_12920"
     CDS             6518..7477
                     /locus_tag="VLF19_12920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HSE94204.1"
                     /translation="MSTLKVVVMGSGGVGGYFGAKLARAGHAVTFVARGAHLDAIRRR
                     GLTVHSAAEGEFSVAVEAAERLDDRSPADLVLITVKCFDTADALAAVRPVVGPDTAVL
                     SLQNGVEAIERIDAALGPGHALGGAAYVFASIEAPGVIVHRFAGRIVLGEPGGGTSPR
                     AERICEAIAKAGIPVEISPDIRPVLWEKYVMICAQAGATAVTRCPTGVVRAVPETWRL
                     FRALAEELAALAAAAGVRLSAGIVDTVVATAAGLAPQTSSSLAFDLSQGKRLELEALH
                     GHAVRLAERLGVAVPSVFAVYAALKPWLDGPPPDPASAGGRDP"
     gene            7632..9305
                     /locus_tag="VLF19_12925"
     CDS             7632..9305
                     /locus_tag="VLF19_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE94205.1"
                     /translation="MGVVCLGTGTVLSLAVPRTVQRAVEALETDAAGAPVGSYVTLIL
                     ALAVASGLARLGSRFSIIGAAQRVEYDVRNDLYASLLRYPPAFYATSSIGDLMSRSTS
                     DVSAVRSLVGFGVVSFISTAFAFVGAVGAMLTVDPRLTVWALAPLSVLVVVARRANAT
                     VHERTRAAQDQLGVLSSRVQEHLSGIAVVRAYTLEPRAEEEFDRANADYLTKSLALQR
                     TLSQFAPLSGLITGLGALIVLWLGGREVIAGRLTLGALVAFNGYLAYLAWPTLALGYT
                     LSMVRRGATSMERIREVVAAAPPPDPPGLALASPPAVSFQHLTFAYPGRPAALTDVSF
                     AVAPGEMVAVVGPTGGGKSTLGLLVARLWDAPPGTVLVDGREVSAWSRPALRGGLGYV
                     PQEAFLFSRSIADNVTLRREDVTPDAARAAAAVAGVADEVERLPSGWDTVVGERGLTV
                     SGGQRQRLALARALAGRPRLLVLDDVFASVDAVKEEEIIVALRAATAGLTVLLMTHRL
                     RAARAADRIVVLAEGRVVETGTHDTLLTQGGAYAQLWRLQQLEEEIARA"
     gene            9298..11082
                     /locus_tag="VLF19_12930"
     CDS             9298..11082
                     /locus_tag="VLF19_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSE94206.1"
                     /translation="MHDVEHDDLLGRAFDRRLIARLAAVARPHARLIAGTTALFPLIA
                     GVELLQPYLLKVAIDEHILTGDWPGLTGVAGLFLAVLVTLAALRAVESYLMQLTGLRV
                     MHDLRALLFRHLLRLEAAFFDRTPVGRLMTRVLNDAEAVSEAFTAGLFAVMGDVVTLA
                     GVVAVMLWMDWRLALVTFSLIPVLGTVAGWFRVHARDAYREVRRRLARLNGFLHESLQ
                     GMTVIQLFARERHETEAFRRLNRDYRRAMFGSTVWEASLYATVEALGSAALALLIWYS
                     AGRIAAETLTFGTLVAFIQYTNRFFLPIRDLGAKYTVMQAAMASSERIFGLLDRAPAI
                     VAPAVPRAPQRRAGDTAAAFQSVWFAYDGEDWVLRDCTFTVASGEHVAIVGATGEGKT
                     TCARLLNRAYDVGRGCVLVDGVDVREWDLPRLRRHVGVVFQDTMLFTGSVEANVRLGA
                     DGRVSRADLERAVDAANCRRVIDALPSGWAEELSERGANLSHGQRQLLAIARALVYNP
                     PVLVLDEATSSVDAESEALIRQAMARLAEGRTTITIAHRLSTVQHADRILVLHRGRVH
                     EEGRHAELLRRGALYARLWELSVRPRSA"
     gene            11097..11270
                     /locus_tag="VLF19_12935"
                     /pseudo
     CDS             11097..11270
                     /locus_tag="VLF19_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
     gene            11334..11462
                     /locus_tag="VLF19_12940"
     CDS             11334..11462
                     /locus_tag="VLF19_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94207.1"
                     /translation="MTDSMLTQRLRLQAEGVALRRGRVALARHRWRPANGGGPKET"
     gene            11464..12480
                     /locus_tag="VLF19_12945"
     CDS             11464..12480
                     /locus_tag="VLF19_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HSE94208.1"
                     /translation="MRLALHLSYSGPTFALDMDKVLEAERLGYDSVWTAEAYGSDAVT
                     PAAWIAARTQRIHVGTAIMQVAARTPAMAAMTAMTLDALSGRRFRMGLGVSNPQVVEG
                     WHGQPFAQPLARTREYVQVVRAIFRREKPLEHRGEHYQIPYAGPHATGLGKPLRSILH
                     GRPELPIYLAAIGPKNVALAAEIADGWIPTFFSPRRMGMFREWLDEGFRARGARPPEF
                     DLMPSVGVVVGERLDECRKPIKARLALYVGGMGARNRNFYNELARRYGYEEAARTLQD
                     LYLSGRKAEAEAAVPDALVDEVALCGPRERIRERLAEWKSAGITTLMVGGNTEAVRAM
                     AELA"
     gene            12494..13435
                     /locus_tag="VLF19_12950"
     CDS             12494..13435
                     /locus_tag="VLF19_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94209.1"
                     /translation="MKVLPLAAESLGVRSMATFVQAGHLAILIDPGATLAANRYGLPP
                     ADEEWEALRRANDRISAYALRADVVFVSHYHEDHFRSDPASYAGRLAVLKDPRRMVQG
                     LQARRALELWKTLPGHARIESADGWTRREPPVSLEASAPLAHGLEGTPLGYVVALTVV
                     DEAERERFVFASDVQGPLSPVVAAWLVRQRPTLLYLSGVPSYVEREVGADTVQRGVEN
                     LRRVIDATGCRVILDHHALRDIRWRERFTDLFATGRVTTAAGHLGLPEAALEAGRQRL
                     WTGVRRPAARVTSSRAIMARHSPSRAASRSAKGGITE"
     gene            13465..13908
                     /locus_tag="VLF19_12955"
     CDS             13465..13908
                     /locus_tag="VLF19_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:13585..13587,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSE94210.1"
                     /translation="MGHRAPDFTLKTIGLKDIRLHDYRGKNVVLLFYPLDWTPGUSTC
                     MPAFDKRIRDFEAANTQVLGISTDSPFSHENWAKSVGIAHYPLLSDIQRTVVKDYGVY
                     WPDWNANSRATFIVDTQGVVRFIERYGKGQLPEPDKILAEVKKLS"
     gene            14012..14455
                     /locus_tag="VLF19_12960"
     CDS             14012..14455
                     /locus_tag="VLF19_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSE94211.1"
                     /translation="MWLAKPRAEVFAFFAEPVNLARITPPWLHFRLLTRAPVLAPFTV
                     LDYRVQWLGVPLRWRTLIREYDPPARFVDVQVRGPYDRWEHRHVFVEERGGTWVEDRV
                     TYRLPLGPLGRAAHGLLVRRQLDGIWAYRRRRIGELIAPALDEPP"
     gene            complement(14547..15179)
                     /gene="nth"
                     /locus_tag="VLF19_12965"
     CDS             complement(14547..15179)
                     /gene="nth"
                     /locus_tag="VLF19_12965"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSE94212.1"
                     /translation="MIRRLERSRPRWAATALAVVAERTRDPYRVLIACILSLRTQDTT
                     TAPAAERLFALADTPAGMLRLSAPTIARTIYPVGFYRTKARVIRAISRDLLERFGGRV
                     PDDIDALLTLDGVGRKTANLVVTMGYGKPGICVDTHVHRISNRLGYVRTRTPEETEMA
                     LRARLPRRYWIGYNDLLVAFGQNVCTPLSPRCSICPARALCRRVGVTTSR"
     gene            complement(15191..15934)
                     /locus_tag="VLF19_12970"
     CDS             complement(15191..15934)
                     /locus_tag="VLF19_12970"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSE94213.1"
                     /translation="MSLRQALPALVLVPMLALPSGAAAQPVADRVRALLVRYDEDPRR
                     IDRARDLLEEALARERPVALLELAARVYFLVGDIRAATPGDKLAAYARGRELGQRAVE
                     LAPRSEDAHLWYAINMGRWGQTKGVLRSLFLLAAMFEEVEAIFAINPRSVRGHALAGN
                     LYFEVPPLFGGDRKKAEQHYRKGLEIEPRFTVLRVDLARLLIATGRVAEARRELARVV
                     AETAPASRADWTVKDRPRARELLDSLKAL"
     gene            complement(15969..16394)
                     /locus_tag="VLF19_12975"
     CDS             complement(15969..16394)
                     /locus_tag="VLF19_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94214.1"
                     /translation="MKPAPAAILDDEARLLALARELTALARGRPPAEALDEALARLPE
                     VERAARHDASHALALGWAREQARLALADVLERAAKAGAARADVAAGTLAWLVLAAAEA
                     VAREPADAAPDRLHALADFVRPPVERESRGFGARPPAPR"
     gene            16485..16862
                     /locus_tag="VLF19_12980"
     CDS             16485..16862
                     /locus_tag="VLF19_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94215.1"
                     /translation="MPFTVDAVPPPWVAAGTGIRVDEAAGVVVLQDGRMDRVGGERRA
                     IVDARPVTISTVRPGTRVVIRAAQAVQLTRPRIYGHRGRAVVRRVYRGAVGFAGDDDG
                     HRNKRADALADRQRSREGYGGEP"
     gene            16849..17037
                     /locus_tag="VLF19_12985"
     CDS             16849..17037
                     /locus_tag="VLF19_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94216.1"
                     /translation="MASHEPLSGLIVFTDGRSFYMGLRAVIMVNDRPVSVDAVRPGTP
                     ITVSGVTRSPPATGVPCS"
BASE COUNT         2404 a         6178 c         6374 g         2498 t
ORIGIN      
        1 acgcatctcg cgcatcagct atggcgagga gcgcggtgtc tgcggcgacc ggaccgacga
       61 ctgctgggcg aagaatcgcc gcgcccactt ctccgtcaag gcgcagtagc gtccatcggg
      121 cgtgcgggga ggagccgagc gctcctcccc ggcgccgacc ttcccccgct cccgcctttc
      181 ccgcgcacac ctcggagccc gtgaacgaat cgcgctgtcg aggggcgcca cagctgccgt
      241 ggcgcaccga gcctcggggg gcttgggggt gtccggaggc ccgtgtactc agtcgtcatc
      301 ggccaggacc acgcaggctt ccggcgcgat ggccagcgtg acggccgcgc cggcctggat
      361 ccgcatggcg gggaggccga ccacgcgaag ggtgaggtca ccgcgctcca gctcgacctg
      421 gtagtgcagg gcgtcgccga ggtaggccgt ccgctggacc gtgccgcgca ggaggttgtt
      481 gcgggaggca acgacctcgg acggccggtc gcccgccgcg atcgcgatct cgtgaggccg
      541 gatcgagacg gccacccgcc ggcccggctc cagatctgcc gcggcgacgc gcaggcgcag
      601 gtcgccacgc tcgacgacgt cccggccgcc ggcgacggca tcgacgaggt tggtcttgcc
      661 gatgaagtcc gcgacgaagc gggtccgtgg ctgctggaac aactcctcgg cggtcccgat
      721 ctgggccacg cggccgcgct gcagtacggc gatccggtcc gagatgacca tggcctcgcc
      781 ctggtcgtgg gtgacgtaga gcgtggtgat tccgaaccgc tcgtgcagcc ggcgaatctc
      841 gaaccgcatt tcctcccgga gatttgcatc gagattgctg agaggctcgt cgagcaggag
      901 gatctccggc tcgacgacga gcgatctggc gacggccacg cgctgctgct gtccgccaga
      961 cagctccccg gggtagcggt cctcgaaccc ggcgagctgg accacctgca gaatctcctt
     1021 cacgcgcgtg gcgcgctccg cccggctcag gtcgcgcttg aaccggagcc cgtacccgac
     1081 gttctgggcc accgtgaggt gcggccagag cgcgtagctc tggaagatca tggccatgcg
     1141 ccgtcgctcg ggtggcacga cggagctcgg cgaggacagg caccggtcgc cgacccagat
     1201 ctcgccggcc tccggcgtga ggaagccggc gacgagcctc agggtggtgg tcttgccgca
     1261 gcccgagggg ccgagcagcg cgaccagttc gcgcggcttg acgtcgagcg agaggctctc
     1321 gacggcggcg aggtcgccat agcgcttggt gagtccccgg acgctcacgc cgggcattcg
     1381 attccatggg gggctccgag cgccccccga gccccccaac gctcggggcg ccccggctgg
     1441 gccgtggcgc ccctcgtcgc cgcgtctcgt gggcgagcgc tcattcccgg acgcccatga
     1501 agtcacgtcc gagcacccgc tgcatcagca ccacggtcgt gaacgtcatc aggagcatga
     1561 agagcccgag cacggcgatg gcgccgaact ggccctcctc cttcagatcg aagatcacga
     1621 cggacatcac cttggtgtcc ggcgtgaaca ggatgatcga ggccgacagc tcgcggatca
     1681 cgccgatgaa gatgaagcac cacgtggcga tgatgccgct cttggcgagc ggcgcggtga
     1741 tctcgcgcag cactcgtagc cgccccgcgc cgagcacgcg cccggcttct tccagctcga
     1801 cgtggacccc cttgaaggtc gcgtcggcct gcgagtagcc gaccggcatc tccttcgtga
     1861 cgtacgcgat gaagaggatc cagatcgtgc cgtagagcag gaagggcggc cgggtgtagg
     1921 cgatgaagag gccgacggcg agcacgacgc cggggatgac cacgggcgcc agcgccagga
     1981 atgccaggac ctggtggccg tacacgaggc gccggttggc gacgtacgcc agcagcgcgg
     2041 cgaggccggc cccgaccgtg gccgtgagga tgcccagctc gagggtgttg aggatggccg
     2101 acttcgtcgc gccgtactcg aagaacgtga aggtgatgtt ggagagcgtg aggttgtccc
     2161 agccgagcgg ctgtgcccac gcccgcgcca gggccgcctt ggtcaggatg ccgtagggca
     2221 ggaagatcga gcacgccatg acggccacgc acgcggccag cgcgggccag cgccaggtgc
     2281 ccaggcggat ggcgcgccgc tggccgccct tgccgccgat cgccgcgtaa cccttgcgtc
     2341 cgagcagccg cttctgggct tgcaggagga gcgcggtcgc cagcaggagc gggatcgaga
     2401 acgccgctgc catctccgcc ttcggcgggt agtggaagaa cgaccagatc tgggtggtga
     2461 tggtgtggaa gccggccggc agcgccagga tggcgggcga gccgaagagc gccagggcct
     2521 gcagcacggc caggatgaaa ccgctgagga tcgccggcgc caccatcggc agggtcaccg
     2581 tggccgcgac gcgcaagcgg ctcgccccga ggatcgaggc cgcctcctcg agatcggacg
     2641 agatcagccc cagcgtgttc gtgatcatga tgtagacgta cggaaatgtg tagatggcca
     2701 cgacgaagac cagccccggc atcgtgaaga tgtcgatgag cgcgtcctcg gcgccggtcg
     2761 ccgcccgcca ggccttgttc agcaggccgg cgttgggccc ggccagcatg acccacgcga
     2821 aggcgcccag aaacggcggc gtcacgaacg aggccatgat gagcccgcgg atcaggccct
     2881 tgccgggcag gtccgtgcgg gccgtgagcc acgccatcgg tgcccctacg acgagcgagg
     2941 cgagacccac cgagagggcg aggacgaccg tgttccagag cgccttctgg agatggccgt
     3001 cggcgaagac gcgcgcgtag tgggacagcg tcgggccccg ctcgctgctg acgctgagcc
     3061 agccgagcca gcccaggggc agcgccatca tcacgacgag cgcgaccgcc gccagcaccc
     3121 acaccgccac cgaccggtcc atcgcgatcg cgcgcatcga ctcctcgtca ggccccgaag
     3181 aactcgacga agcgcttctt gatctcctcg ttccgcttct cgagctcgtc cggatccacc
     3241 tgcaggagct tcagctccgt gagcctgggc ttgtcggccg ggtacgtcac ccgagggtga
     3301 ccggtataga gaccctcgct gtcggccatg acctgctgga gctcgcgggt gaacgtgaag
     3361 tcggtgaaga gcttcgccgc gttcgggtgc ggcgcgaagg ccgtgatcgc cgtcggcgaa
     3421 accacgagcg gcacgccctc tttcgggtag acgagctcga tgggcgctcc cttcttcttc
     3481 gcctggtagg cgctgtagtc gccgccgttg acggcgacct gccgctctcc ggccgccacc
     3541 acgcccggag ggtcggccgc cgattgcacg agcatcacgc ggttctgggc gagctgcttg
     3601 aagtagtccc agccgtgcag gtgcacgagc gccagcacgt gggtggcgat caccccgctg
     3661 tagcccggat gcgccgagac cagcttgccc ttccacttcg ggtcgagcag gtccttccac
     3721 gtcttcggcg cctcggctgc cggcacgagc gtggtgttgt aggcgatcgc gttgacggtg
     3781 gcgcgcaggc cgaagtggta gccgtccctg tccttgaagc ctaccgggaa gctgtccact
     3841 ccgggcggcg tgtacttcat gaggagcttc ttgtccttca acagcacgaa gtgcccggca
     3901 tcggaggtgt gcacgacgtc ggcgttcctg atgttggcct gcagctcctg catcagccgc
     3961 tgcaggacac gctgggaccc cgtccggtgc acctcgacct tgatccccgg gtgggcggcc
     4021 tcgaacatct tggcgacctt ttcggcgctc ggcaggacga gcgacgtgta ccagacgacc
     4081 ttgccttcct tcttcgcggc ctccacgcgc gcgtcctggg cgcgcgcggc cgggatgctg
     4141 gcggtgagcg ccagggcgcc cgtgacggtc agggtcacga actcgcgtcg atccatccgc
     4201 gtctctgtca ctggagggcc tccatgagat cgtcgagcgg cagcgcctgg cccagcccgc
     4261 cgacctccgc gatgatgcgg tcgatgcgtg ccgccggcag cacgagcccg gcgttgccgc
     4321 ggaattttgc ctccagctcc gcggccgtca aggggtggtc ggcgcccccg cgggggtggt
     4381 tctggcgggc gtccagcacc cgcccgtcgc gcaggcgcac cgacacctgt ccgatgaact
     4441 gccgggggta gtcgatcgtt tcgtcgaggt cgtacgacac ccggccggcc agccgcaaca
     4501 ccgcctcgtc gcgcacggcc tcgtcggtga actcggcgag tgaggcccgc ccgcggatca
     4561 ggatgaccgc gatcagatag ggaaggctga acttggcggc gtaaccgttc ggcggccggt
     4621 gcttggcggc gagcggctcc cacaagcgcg gcaccggacc cgcggccgtc cggcagcgga
     4681 tctcgacgac gtcgtccgga gcgatcgcgt gctcgctccg cagtcggagc gcgcagtcca
     4741 tgtacggctg ggcgatcgag ccgcacggga agggcttgaa cgtcagacgc tcgatctccc
     4801 actcgcggcc gagcgtgccg agcagggcct cgagcgtgac ctcgttgtga ccgtcggcga
     4861 aggccgcgta gaagccgtgg cggccctcca ggacgtgttc cggtccggtg aagccgtcgc
     4921 gggcgagcag ggccgccacg accccggcat gggccgccca gccgggatgc aggcgcttcg
     4981 tccacgtgcc gtccgtcagg tactcgatga tcccgctcgc ctggctgccg cagatgccga
     5041 aggcgttgac caggcggtcc tcgtcgagac ggtagaggcg gccggccgcc gcagcggcgc
     5101 cgaagacgcc ggtgaccgcc gtggggtgga agtgccgcgc gtggagccgg ccgggaacgg
     5161 cgaggccgac gcggcacatg acctcgacag cggcgacgag cgccgtgagc accgcggcgc
     5221 cggaggcggc tgtcgcctgg gccagggcga gggccaccgg cacggcgaca catcccgtgt
     5281 gcacgatcgc gtcctcgcgc gtgtcgtcga agtccaggcc gtgggcgagc gtcgcgttgg
     5341 cgagcacggc attcgccgcg cccacacggg cgccgccgcc gatcagcgtc gactcggccg
     5401 ggccgccgag acctcgcgcg gtcgccagcg cagcgcgcgc gaagtccatg gaggaggcgg
     5461 cgagcgcgca gccgaggatg tcgagcgtga gccgtcgggc gcgctccacc accgaagccg
     5521 gcacgctggc gaggtcgagt ccgagcacga agcgcgcgag cctccgcgcc gtcgagacgt
     5581 cgcccatcag cggtcgttca tccgcggatc tccttcagca cggccagcac ttcgtcgtgg
     5641 atccgtccgt tggttgcgac gatggcgggc ctgtccaggt cgagcgggcc gccgccgagg
     5701 tcggtgaccc ggcctccggc ctcctcgacc agcaaggcgc cggccgcggc atcccaggcg
     5761 cccagcgtga gctcccagaa cccatcgtag cggccggccg ccacccaggc gaagtcgatc
     5821 acggcggagc cgagccgccg cacggcacgg gcgcggacgc tgaaggccgc gtactcgcgc
     5881 aggttgttgt ccgcgatctg gcggatgctg taggggaagc ccgtggtcaa caggctctcg
     5941 tcgagcacgc tcgtcgccga caccgcgagc cgctcctcgc gcaggaacgc gccgccgccg
     6001 cgctcggcga cgaacagttc ctcgaggttc gggtcgtaga cgacgccgag cactgcgcgg
     6061 ccggcggcct cgagcgcgat ggagaccgcg aagacgggga cgccgtgcgc atagttggtg
     6121 gtgccgtcga gcgggtccac gatccagcgc cggccggagc gccccggttg cgcgcccgcc
     6181 tcctcggaca gcacgccgtc gtcggggaag gccgccacga ggcgctggag gatcagtgct
     6241 tccgcgcgcg cgtccatctc ggtgacgagg ttggtgggag cgcccttgta ggtgatccgc
     6301 cgcggccctc gcacctctct ccggagcagg tcgccggcgt cgcgcgcggc gtcgatcgcc
     6361 acgcgccgct cgagggaggc catgccgccg cgagcgtatc acagttgccc cccaccttcg
     6421 gagcaccccg tccgagccgg ggtgcgcctc tcatctgatg ccgcgttcca gtctgcggct
     6481 tgacaaccaa cggagcctcc cggagcatga gcatctcgtg agcacgctga aggtcgtcgt
     6541 gatgggctcg ggcggcgtcg gcggctactt cggtgccaag ctcgcccggg ccggccacgc
     6601 tgtgaccttc gtcgcgcgcg gtgcccacct ggacgcgatc cgccgccgtg gcctcaccgt
     6661 ccactcggcc gccgagggcg agttcagcgt cgccgtggag gccgccgagc gcctcgacga
     6721 ccggtcgccg gccgatctcg tgctgatcac cgtgaagtgc ttcgacaccg cggacgccct
     6781 tgccgccgtg cgccccgtcg tcgggccgga caccgcggtg ctctcgttgc agaacggcgt
     6841 ggaggccatc gagcgcatcg acgccgcgct cgggcccggc cacgcgctcg gcggcgccgc
     6901 ctatgtgttc gcgtccatcg aggcccccgg ggtcatcgtc caccggttcg cgggacggat
     6961 cgtgctgggc gagccgggcg gcggcacgag cccgcgtgcc gagcgcatct gcgaagcgat
     7021 cgccaaggcc ggcatccccg tggagatctc gcccgacatc cggccggtgc tgtgggagaa
     7081 gtacgtcatg atctgcgccc aggccggagc gacggcggtg acgcggtgtc cgaccggcgt
     7141 cgtgcgtgcg gtgcccgaga cgtggcggct gtttcgcgcg ctggccgagg agcttgcggc
     7201 gctcgccgcg gccgcgggcg tccggctgtc ggccggcatc gtggacacgg tcgtggccac
     7261 tgccgcgggg ctcgcgccgc agacgagctc gtccctcgcc ttcgacctga gccagggcaa
     7321 gcgcctggag ctcgaagccc tgcacggcca cgccgtccgg ctcgccgaga ggctcggcgt
     7381 ggccgtgcca tcggtgttcg ccgtgtacgc ggcgctcaag ccctggctcg acgggccgcc
     7441 accggaccct gcgtccgccg gcggccgtga cccgtgaacg aagcgcggac gccacggctg
     7501 cgccggggtg caccgagcgt tcggggcgct cgggggacgc tcggaggccc ccacatgacg
     7561 cggggcgcgc gctgacgtcc gccgcgcgcg ccctctggca ctacgccggc cgctaccgcc
     7621 ttcgctacgc cgtgggtgtc gtctgcctcg gaaccggcac cgtgctgagc ttggccgtgc
     7681 cccgcacggt ccagcgggcc gtggaggcgc tggagaccga tgccgccgga gcgcccgtcg
     7741 gctcctacgt gacgctgatc ctggcgctcg ccgtggccag cggcctggcg cggctgggct
     7801 cgcggttctc catcatcggc gcggcacagc gggtcgagta cgacgtccgc aacgatctct
     7861 acgcgagcct gctccgctac ccaccggcct tctacgccac gagctcgatc ggcgacctca
     7921 tgtcgcgatc caccagcgac gtgagcgccg tgcgctccct ggtcggcttc ggtgtcgtca
     7981 gcttcatcag cacggccttc gcgttcgtcg gcgcggtcgg cgcgatgctc accgtcgacc
     8041 cccggctgac ggtctgggcg ctggcgccct tgtcggtcct ggtggtcgtg gcgcggcggg
     8101 ccaacgccac agtgcacgag cgcacgcgcg cggcccagga ccagctcggc gtgctgtcgt
     8161 cgcgcgtgca ggagcacctg agcggtatcg ccgtcgtccg cgcctacacc ctcgagcccc
     8221 gtgcggagga ggagttcgat cgcgccaacg cggattacct cacgaagagc ctcgccctgc
     8281 agcgcacgct ctcgcagttc gcaccgctgt ccgggctgat caccgggctc ggggccttga
     8341 tcgtgctgtg gctgggcggc cgcgaggtga tcgccggacg gctgaccctg ggggcactcg
     8401 tggcgttcaa cggctacctg gcgtacctgg cctggcccac gctggcgctc ggctacacgc
     8461 tctcgatggt gcgccgcggc gcgacgtcga tggagcgcat ccgcgaggtc gtcgccgcgg
     8521 cgccaccgcc cgaccccccc ggcctcgccc tcgcctcgcc gccggcggtg agcttccagc
     8581 acctgacgtt cgcgtatccg ggacgtccgg ccgcgctgac ggacgtgagc ttcgccgtcg
     8641 cgccgggcga gatggtggcc gtcgtcgggc ccaccggcgg cggcaagtcc accctgggct
     8701 tgctggtggc gcgcctctgg gacgcgccgc cgggcaccgt gctcgtggac ggccgggagg
     8761 tttcggcgtg gagccggccg gcgctgcgcg gcggactcgg ttacgtcccg caggaggcgt
     8821 tcctcttctc ccggtcgatc gccgacaacg tgacgctccg gcgagaggac gtgacgcccg
     8881 acgccgcgcg ggccgccgcg gcggtcgccg gcgtcgccga cgaggtcgag cgcttgccgt
     8941 cgggctggga cacggtcgtc ggcgagcgcg gcctgacggt ctcgggcggc cagcgccagc
     9001 ggctggcgct ggcgcgcgcg ctcgccgggc ggccgcggct gctggtgctc gacgacgtct
     9061 tcgcgagcgt ggatgccgtc aaggaggagg agatcatcgt cgctctgcgc gcggcgacgg
     9121 cggggctcac ggtgctcctg atgacccacc gcctgcgcgc cgcgcgcgcc gccgaccgca
     9181 tcgtcgtgct ggccgagggc cgcgtggtgg agaccggtac gcacgacacg ctgctcacgc
     9241 agggcggcgc ctacgcgcag ctctggcgac tgcagcagct cgaggaggag atcgcgcgtg
     9301 catgacgtcg agcacgacga cctcctgggg cgcgcgttcg accggcgcct catcgcgcgc
     9361 ctggccgccg tggcgcggcc ccacgcccgc ctgatcgcgg ggaccacggc gctcttcccg
     9421 ctcatcgccg gtgtcgagct gctgcagccg tacctcctca aggtcgccat cgacgagcac
     9481 atcctgaccg gcgactggcc cgggctgacc ggcgtcgccg gcctgttcct ggcggtgctg
     9541 gtgactctcg ccgcgctccg cgccgtcgag tcgtacctga tgcagctcac cggcctgcgc
     9601 gtgatgcacg acctgcgcgc gctgctcttc cgccacctgc tgcggctgga ggcggccttc
     9661 ttcgatcgca cgccggtcgg ccggctcatg acccgtgtcc tcaacgacgc cgaggcggtg
     9721 agcgaggcgt tcacggccgg gctgtttgcg gtgatgggcg acgtggtcac gctcgccggc
     9781 gtcgtcgcgg tgatgctctg gatggactgg cggctggcgc tggtgacgtt ctcgctgatc
     9841 cccgtgctcg gcaccgtcgc cgggtggttc cgggtgcacg cgcgcgacgc ctaccgggag
     9901 gtgcggcggc gcctggcccg cctcaacggc ttcctgcacg agtcgctcca gggaatgacg
     9961 gtgattcagc tcttcgcccg cgagcgccac gagacggagg cctttcgtcg tctgaatcgt
    10021 gactaccggc gcgcgatgtt cggctcgacg gtctgggagg cgtcgctcta cgcgacggtc
    10081 gaggcgctcg gctcggcggc gcttgccctg ctcatctggt acagcgccgg ccgcatcgcc
    10141 gccgagacgt tgacgttcgg cacgctggtg gccttcatcc agtacacgaa ccgcttcttc
    10201 ctccccatcc gcgacctcgg cgcgaagtac acggtgatgc aggcggcgat ggcctcgtcc
    10261 gagcgcatct tcgggctcct cgaccgcgca ccggcgatcg tggcgccggc cgtgccgcgc
    10321 gctccccagc gacgggcggg ggacacggcc gccgccttcc agtcggtgtg gttcgcctac
    10381 gacggcgagg actgggtgct ccgggactgc acgttcacgg tggcgtcggg cgagcacgtg
    10441 gcgatcgttg gcgccaccgg cgagggcaag acgacgtgcg cgcgcctgct caaccgtgcc
    10501 tacgacgtgg ggcggggttg cgtgctcgtc gacggcgtgg acgttcgcga gtgggacctt
    10561 ccgcgcctgc gacggcacgt cggcgtcgtc ttccaggaca cgatgctctt caccggcagc
    10621 gtggaggcca acgtccgcct gggcgccgac gggcgcgtga gccgcgccga cctcgagcgc
    10681 gcggtggacg ccgccaactg ccgccgcgtc atcgacgcgc tcccgagcgg ctgggcggag
    10741 gagctgtccg agcgcggcgc gaatctctcg cacggtcagc gccagctcct ggccatcgcc
    10801 cgcgccctcg tatacaatcc tcccgtcctc gtcctggacg aggcgacgtc gagcgtcgac
    10861 gcggagtccg aagcgctgat ccgacaggcc atggcgcggc tggcggaggg tcgaacgacc
    10921 atcacgatcg cccaccggct ctccaccgtc cagcacgccg accgcatcct cgtgctccat
    10981 cgcgggcgtg tccacgagga gggccggcac gccgagttgc tgcgccgggg agcgctctac
    11041 gcgcggctgt gggagctgtc ggttcgcccg cggtcggcct gacgttcgcg cgcgcggtgt
    11101 ggcggctcgt gcgacgcgtt ccgcgcgggc gggtggtcac gtacggacag atcgcggcgc
    11161 tcctcgggca gcccggcgcc gcgcgccgtg tcggctgggc gatgcggatc tgcccggccg
    11221 acctgccatg gcaccgcgtg gtgaacgcgc agggaaggat cagcgtgcga ggccgtggcc
    11281 gggcgaaccg cgggacgacg cgaggcggga gtcacgcggt ccgaggcggg ccgatgaccg
    11341 atagcatgct gacgcagcgc ctgcgcctgc aggcggaggg cgtggcgctc cgacgaggcc
    11401 gcgtggcgct ggcgcgccac cggtggcggc ccgcgaacgg cggagggccg aaggagacat
    11461 gagatgcggc tggcgctcca tctgagctat tccggtccca ccttcgccct cgacatggac
    11521 aaggttctgg aggcggagcg tctcggctac gactcggtgt ggacggccga ggcctacggc
    11581 tccgacgccg tcacgccggc cgcgtggatc gccgcacgca cgcagcgcat ccacgtcggc
    11641 acggcgatca tgcaggtcgc cgcgcgaacg ccagcgatgg ccgcgatgac ggcgatgacc
    11701 ctcgacgccc tgtcgggtcg ccgcttccgc atgggcctcg gcgtctcgaa cccccaggtc
    11761 gtcgagggct ggcacggcca gcccttcgcc cagccgctgg cgcgcacgcg cgaatacgtg
    11821 caggtcgtcc gcgccatctt ccggcgcgag aagccgctcg agcaccgggg cgagcactat
    11881 cagattccgt atgccggacc ccacgccacc ggcctcggca agccgctgcg ctcgatcctg
    11941 cacggacggc ccgagctgcc gatctacctg gcggcgatcg ggccgaagaa cgtcgccctc
    12001 gctgccgaga tcgccgacgg ctggattccc accttctttt cgccccgtcg gatgggaatg
    12061 ttccgcgagt ggctggacga gggcttccgc gcccgcggtg cccggccgcc ggagttcgac
    12121 ctgatgccgt cggtcggcgt cgtcgtcggg gagcgcctcg acgagtgccg caagcccatc
    12181 aaggcgcgcc tggcgctcta cgtcggcggc atgggggcgc ggaacaggaa cttctacaac
    12241 gagctggcgc gccgctacgg ctacgaggag gccgcgcgga ccctgcaaga cctgtatctc
    12301 tcggggcgga aggccgaggc cgaagccgcg gtgcccgacg ccctggtgga cgaggtcgcc
    12361 ctctgcgggc cgcgggagcg cattcgcgag cggctcgcgg agtggaagtc ggcaggcatc
    12421 accacgctga tggttggggg caacaccgag gccgtgcgcg cgatggccga gctggcgtaa
    12481 ccgcgtccgt cacatgaagg tcctgcccct cgccgccgaa tcgctcggcg tccggtccat
    12541 ggccaccttc gtccaggccg gtcacctcgc aatcctcatc gaccccgggg cgacgctcgc
    12601 ggcgaaccga tacgggctgc cgccggccga cgaggagtgg gaagcgctgc ggcgcgccaa
    12661 cgaccgcatc tcggcctacg cgctgcgcgc cgacgtcgtc ttcgtgagcc actaccacga
    12721 ggaccacttc cgctccgacc cggcgagcta cgccggacgc ctggccgtgt tgaaggatcc
    12781 gcgccggatg gtgcaaggac tccaggcccg tcgcgcgctt gagctgtgga agacgctccc
    12841 ggggcacgca cggatcgaga gcgccgacgg ctggacgcgc cgcgagccgc cggtgagcct
    12901 cgaggcctcc gcgcccctcg cgcacggcct ggagggcacg ccgctcggct acgtcgtggc
    12961 gctgaccgtc gtggacgagg cggagcgcga gcggttcgtg ttcgcctccg acgtgcaagg
    13021 gcccctctcg ccggtggtcg ccgcctggct cgtgcgccag cgaccgacgc tgctgtacct
    13081 gtccggagtc ccgtcgtacg tggagcgcga ggtcggggcc gacaccgtgc agcgcggcgt
    13141 ggagaacctt cggcgtgtca tcgacgcgac ggggtgccgg gtgatcctcg atcaccacgc
    13201 gctccgggac attcgctggc gcgagcgctt cacggacctc ttcgccaccg gccgcgtgac
    13261 gaccgcggcg gggcacctcg ggctcccgga agcggccctc gaggcaggtc gccagcgact
    13321 ctggacgggc gtgcgaaggc cggcggcgcg ggtcacgagc tcgcgtgcta tcatggcgcg
    13381 gcattcaccg tcgcgcgccg ccagccgatc cgcgaaaggg ggaatcaccg agtgagcagc
    13441 gccaccgaga acaagacgct cgacgtggga caccgggcac ccgacttcac gttgaagacc
    13501 atcgggctca aggacatccg cctgcacgac tatcgcggca agaacgtcgt gctgctcttc
    13561 tacccgctcg actggacgcc gggctgaagc acgtgcatgc ccgccttcga caagcgcatt
    13621 cgcgacttcg aggcggccaa tacccaggtg ctgggtatca gcacggacag ccccttcagc
    13681 cacgagaact gggcgaagtc ggtcggcatc gcgcactacc cgctgctgtc cgacatccag
    13741 cgcaccgtcg tcaaggacta cggcgtgtac tggcccgact ggaacgcgaa cagccgtgcc
    13801 acgttcatcg tggacacgca gggggtcgtg cgcttcatcg agcgttacgg caagggccag
    13861 ctgcccgagc ccgacaagat cctcgccgag gtgaagaagc tgagctgacg ccgatcccgg
    13921 tcgccgtgtc gtctcgcgcg agccgggggg cctgcggggg ggcgcctggc ccccccgttc
    13981 ttcgttgatg gaccatattc tcgaggtacg ggtgtggctc gcgaagccgc gtgcggaagt
    14041 cttcgccttc ttcgccgagc ccgtcaacct cgctcggatc acgccgccct ggctccactt
    14101 ccgcctgctg acccgcgcgc cggtgctggc cccgttcacc gtgctcgact accgtgtgca
    14161 gtggctcggg gtgccgctgc gctggcgcac cttgatccgc gagtacgatc cgccggcgcg
    14221 gttcgtggac gtgcaggttc gcgggcccta cgaccggtgg gagcaccgcc acgtcttcgt
    14281 cgaggagcgc ggcgggacgt gggtcgaaga ccgcgtgacc taccggctgc cgctggggcc
    14341 gcttggccgc gcggcgcacg ggcttctcgt gcggcgccag ctcgacggca tctgggcgta
    14401 ccggcgccga cggatcggcg agctgatcgc acccgcgctg gacgaaccgc cgtgaggggt
    14461 gggcgcgcgc ccgttcggtg ctcgagatgc cgtgagcatg cattcgagga cggagcgcgc
    14521 aggccggtcc ttcgatgtgg gctggatcag cgtgaggtcg tgaccccgac gcgacggcag
    14581 agtgcgcgcg cagggcagat cgagcagcgg ggcgacaggg gcgtgcagac gttctggccg
    14641 aacgccacga gcaggtcgtt gtagccgatc cagtaccgcc gcgggagccg cgcccgcagc
    14701 gccatctcgg tctcctcggg cgtgcgcgtg cgcacgtagc ccaggcggtt cgagatgcgg
    14761 tggacgtggg tgtccacgca gatgccgggc ttgccgtacc ccatcgtgac gaccagattc
    14821 gctgtcttgc gaccgacgcc gtccagggtg agcagcgcgt cgatgtcgtc ggggacacgg
    14881 ccgccgaagc gctccagcag atctcgcgag atcgcccgga tgacgcgggc cttcgtgcgg
    14941 tagaagccga cgggatagat cgtccgcgcg atggtcggcg ccgacaggcg gagcatcccc
    15001 gccggggtgt cggcgagcgc gaacagccgc tcggcggcgg gcgccgtggt cgtgtcctgc
    15061 gtccgcagcg acaggataca ggcgatcagc acgcggtagg ggtcgcgcgt gcgttcggcg
    15121 acgacggcga gcgccgtcgc ggcccaccgc gggcggctcc gctcgagccg ccggatcacg
    15181 gcgccgatcg ctacagggcc ttgagcgagt cgagcagctc gcgggcgcgt gggcggtcct
    15241 tgacggtcca gtcggcgcgg ctcgccggcg cggtctccgc gacgacccgg gcgagctcgc
    15301 gccgggcctc ggcgacacgg ccggtggcga tgaggagcct cgccaggtcg actcgcagca
    15361 cggtgaagcg gggctcgatc tcgagaccct tgcggtagtg ttgctccgcc ttcttccggt
    15421 ccccgccgaa cagcggcggc acctcgaagt agaggttgcc ggcgagcgcg tggccacgca
    15481 ccgatcgcgg attgatcgcg aagatggcct ccacctcttc gaacatcgcc gccagcagga
    15541 acagcgagcg tagcaccccc ttcgtctggc cccagcggcc catgttgatc gcgtaccaga
    15601 gatgcgcgtc ctcgctgcgt ggcgcgagct ccaccgcccg ctgccccagc tcccggccgc
    15661 gtgcgtaggc ggccagcttg tcgccgggcg tcgccgcacg gatgtcgccg acgaggaaat
    15721 acacgcgggc ggcgagctcc agcagcgcca ccgggcgctc gcgggcgagg gcctcctcca
    15781 ggaggtcgcg agcgcggtcg atgcgtcggg gatcctcatc gtaacgcacg agcagggcgc
    15841 gcacgcgatc ggcgacgggc tgggcggcgg cgccggaggg cagggcgagc atggggacga
    15901 gtacgagcgc cgggagcgcc tgacgcagcg acatcgcggc gcactctacc gaagcgcggc
    15961 gcgcggtgtc aacgcggtgc cggcggccgc gcgccgaacc cgcgcgactc gcgctccacg
    16021 ggcggacgga cgaaatccgc gagggcgtgc aggcggtcgg gcgcggcgtc ggccggctcg
    16081 cgggcgacgg cctcggcggc cgcgagcacc agccaggcga gggtgcccgc ggcgacgtcg
    16141 gcgcgggctg cgccggcctt cgcggcccgc tcgaggacgt cggcgagcgc caggcgcgcc
    16201 tgctcgcgcg cccagccgag cgccagcgcg tggctcgcgt cgtggcgcgc cgcgcgctcc
    16261 acctccggga gccgggcgag cgcctcgtcg agcgcctctg ccggcggccg tccgcgcgcg
    16321 agcgccgtca actcgcgcgc gagggcgagc agcctcgctt cgtcgtcgag gatggccgcg
    16381 ggagcgggct tcacgggcgg agcgaccttg gctcgcacgc ggcggcgctt cggccgacgg
    16441 cgcttcgatc gcctgtccgc catgtccgct ccacgataac agaggtgccg ttcacggtgg
    16501 atgccgtgcc gccgccctgg gtggccgcgg gcaccgggat acgcgtggac gaggccgcag
    16561 gtgtcgtcgt cctgcaggac gggcggatgg accgggtcgg gggcgagcgc cgcgcgatcg
    16621 tggacgcccg acccgtcacg atctcgacgg tgcgcccggg cacgagggtg gtgatccgtg
    16681 ccgcgcaggc cgtccagctt acgcgaccga gaatatatgg tcatcgaggc cgcgccgtcg
    16741 tccggcgtgt ctatcgcggc gccgtcggct tcgccggcga cgacgacggt caccgtaaca
    16801 agcgcgccga cgcccttgct gatcgccaac gaagtcgtga agggtacggt ggcgagccat
    16861 gaaccgctga gcggcctcat cgtgttcacc gacggccggt cgttctacat gggcctgcgc
    16921 gccgtcatca tggtgaacga ccgcccggtc tcggtcgacg cggtccggcc gggtacgccg
    16981 atcacggtga gcggagtgac ccggtcgcct cccgcgacgg gcgttccgtg ctcctgagcg
    17041 agggcttccg tgactcggcg agagcccgat ggcgcccgac gcgaaatgcg tgagtcacgc
    17101 ggtcgaggcg gcggacgccg cgatgcagat ccaggccggc ggataggtcg agccgcagac
    17161 aggtcctgcg tgcggggggc acgtcgatcg aaggcacggc gccgccgagg gccggggaaa
    17221 gcctgggacg ccgggtgagc ggtatcgacg cccaccggcc gcaacgcttc ccgggtccgt
    17281 cactcgtacc gcagcgcttc gatcggatcg agccgcgcgg ccttgcgagc gggatagaag
    17341 ccgaaaaaga tcccgatcgt cgcggagaac ccgaaggcga gcgcgatcgc ttccggggcc
    17401 accagcgtcc gccacgccgc caggtagctg atggcccgcg agccaccgag tccc
//
LOCUS       DATIAZ010000377         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_391744, whole genome shotgun sequence.
ACCESSION   DATIAZ010000377 DATIAZ010000000
VERSION     DATIAZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391744"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VLF19_12990"
     CDS             <1..214
                     /locus_tag="VLF19_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSE94217.1"
                     /translation="NSVHPGPVDTEMFHSAFRDPEALERRLQRIPLGRMGTVAEIVSA
                     VLYLASDESSYMTGSELIIDGGALAQ"
     gene            352..1512
                     /locus_tag="VLF19_12995"
     CDS             352..1512
                     /locus_tag="VLF19_12995"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HSE94218.1"
                     /translation="MAPAWDLLIRGGTLVDPAQSVSARRDIAFRGGKVAAVAQTLTGE
                     ATEAIDATGALVTPGLIDIHTHVYAGLSTGRHADQTSLANGVTTVVDAGSAGWMTLPG
                     LRDYVVPTYRTRVYAFLHISATGLTINRIMPELAEIKFAQVEEAARAATENRALVLGI
                     KVRIAHGATGQGNQANAREALRRGRQAADLAGVRLMVHVSDTPIPLDEILDHLREGDI
                     ATHIFNGNVEQVLGPDGRVRGAVRAAQERGVVLDVGHASIHCDAKVARRAIGEGLLPT
                     TLSTDLHTPPPGRIVYNLRAIMSKFLALGMTLDDVVASVTSRAAAAVGKSAELGSLAP
                     GMAGDAAVMDLEDGHFTYVDGARNEVKATRRFRTRHVIREGRRLPAAHADYV"
     gene            complement(1544..>2642)
                     /locus_tag="VLF19_13000"
     CDS             complement(1544..>2642)
                     /locus_tag="VLF19_13000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HSE94219.1"
                     /translation="VVKIESRAGDETRGQLADPTWAERKMAPSFLAVNGNKRSITLDL
                     RRPEAVEIVKRLVVGADVVWENFRGGVMDRLGLGYDALSAINPRLIYCGVSGFGRTGP
                     ERATAAFDGKLQAMSGIMSITGEPASGPTRAGFAICDTIGGMTAALAVASALYQRTHT
                     GRGQLVDVAMLDAALAFIPGPVSEYTVAGIEQRQIGNGSVSRKPTASRFRARDGYIVL
                     AVLTEKQFASLMRTLGRPDALDDPRFKDWPARTENEAALREIIESALATDDPKTWEAR
                     LTAADVPCGSIWKIHEIVHHPQLEHRDVLQTIDSRYGRMRLVGAGFRLAHGSPGLDRE
                     PPTLGEHTDEILNEAGYGAEEIARLRRDAVI"
BASE COUNT          386 a          959 c          924 g          373 t
ORIGIN      
        1 caactccgtg catccgggcc cggtcgacac ggagatgttc cacagcgcct tccgggaccc
       61 ggaggccctg gagcgacggc tgcagcgcat cccgctcggg cggatgggca cggtggccga
      121 gatcgtgagc gccgtgctct acctggcgtc ggacgaatcg tcgtacatga ccggaagcga
      181 gctgatcatc gacggcggcg cgctcgcgca atagtgcagg aggagatgga cgcgtcaggc
      241 ggcgccacgc cctgaccgcg ggagagggct gccacacgcc ccgcgtgggt cgactggatc
      301 agtccacgcc cggccccgtc cgctcgtgca cctcaaacgg gggagcctca gatggcacca
      361 gcctgggacc tgctcatccg cggcgggacg ctcgtcgatc ctgcccagtc cgtctcggcg
      421 aggcgagaca tcgcattccg cggcgggaag gtggcggccg tggcgcagac gctgaccggc
      481 gaggccaccg aggcgatcga cgcgacgggt gcgctggtca cgcccgggct catcgacatc
      541 cacacccacg tctacgccgg actctccacg ggccgccatg ccgaccagac gtcgctcgcc
      601 aacggcgtca ccacggtggt cgacgcgggg agcgccggct ggatgacgct ccccgggctc
      661 cgcgactacg tcgtcccgac ctaccggacg cgcgtctacg cgttcttgca catcagcgcg
      721 accgggctca cgatcaaccg gatcatgccc gagctcgcgg agatcaagtt cgcccaggtc
      781 gaggaggccg cacgcgccgc gaccgagaac cgcgcgctcg tcctcggcat caaggttcgc
      841 atcgctcacg gcgcgaccgg ccagggaaac caggccaacg cgcgcgaggc gctgcggcgg
      901 gggcgacagg cggccgacct ggccggtgtc cgtctcatgg tgcacgtctc cgacacgccg
      961 atcccgctcg acgagatcct cgatcacctc cgcgagggtg acatcgccac ccacatcttc
     1021 aacggcaacg tcgagcaggt gctcggtccc gacggccgcg tgcgcggtgc ggtccgggcg
     1081 gcgcaagagc gcggcgtggt gctcgacgtg ggccacgcct cgatccactg cgacgcgaag
     1141 gtggctcggc gagcgatcgg cgaaggcctg ctgcccacga ccctcagcac cgatctccac
     1201 acgccgccgc cggggcggat cgtctacaac ctccgggcga tcatgtcgaa gttcctggcc
     1261 ctcggcatga ccctcgacga cgtggtggcc tcggtgacga gccgggcggc cgccgccgtc
     1321 ggcaagtcgg cggagctcgg atcgctcgcg ccggggatgg ccggcgacgc cgccgtcatg
     1381 gacctcgagg acgggcactt cacctacgtg gacggcgcgc gcaacgaggt gaaggccacg
     1441 cgtcgcttcc ggacgcgcca cgtgatccgc gaaggccgcc ggctccccgc ggctcacgcc
     1501 gactacgtgt gagcgacggc acgacgcgcg ggcgcagccg gggtcagatc accgcgtcgc
     1561 gccgcagccg cgcgatctcc tcggcgccgt agccggcctc gttcaagatc tcgtcggtgt
     1621 gctcgccgag cgtcggcggc tcgcgatcga gaccggggct gccgtgcgcg agccggaacc
     1681 cggcgcccac gagccgcatg cgaccatagc gcgagtcgat cgtctggagc acgtcgcggt
     1741 gctcgagctg cgggtggtgg acgatctcgt ggatcttcca gatggatccg cacggcacgt
     1801 cggcggccgt cagccgcgcc tcccacgtct tggggtcgtc ggtcgccagc gcgctctcga
     1861 tgatctcgcg cagcgccgcc tcgttctcgg tgcgtgcggg ccagtctttg aatcgtggat
     1921 cgtcgagggc gtcggggcgc cccagcgtcc gcatgaggct cgcgaattgc ttctcggtga
     1981 gtaccgccag cacgatgtag ccgtcacgtg cccggaagcg gctcgcggtc ggtttccggc
     2041 tgaccgaccc gttgccgatc tgacgctgct cgatgccggc cacggtgtac tcggagaccg
     2101 gcccggggat gaaggcgagc gccgcgtcga gcatggcgac gtccacgagc tggccgcgcc
     2161 cggtatgcgt gcgctggtag agcgcgctcg ccacggcgag cgccgccgtc atgccgccga
     2221 tcgtgtcgca gatggcgaac cccgcacgcg tcgggccgct ggcgggctcg cccgtgatcg
     2281 acatgatccc cgacatggcc tgcagcttgc cgtcgaaggc ggccgtcgcg cgctccggtc
     2341 ccgtccgacc gaagcccgag acgccgcagt agatgagccg cgggttgatg gccgagagcg
     2401 catcgtagcc gagcccgagc cggtccatga cgccgccacg gaagttctcc cacaccacgt
     2461 cggcgccgac caccagccgc ttgacgatct ccaccgcctc gggccgccga aggtcgagcg
     2521 tgatgctgcg cttgttgccg ttcaccgcca ggaaggacgg cgccatcttg cgctccgccc
     2581 acgtggggtc ggcgagctgc ccgcgcgtct cgtctcccgc gcgcgactcg atcttgacga
     2641 cg
//
LOCUS       DATIAZ010000378        10628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_259778, whole genome shotgun sequence.
ACCESSION   DATIAZ010000378 DATIAZ010000000
VERSION     DATIAZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259778"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6027)
                     /locus_tag="VLF19_13005"
     CDS             complement(<1..6027)
                     /locus_tag="VLF19_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSE94220.1"
                     /translation="MPALALLVRLALVVAAVVALWGAGAGALLRQAAGREEARVEEVV
                     AAARAAFDEEAQALAREAAMLARDGAVVDGAARGDLAALTRSTSPRMLTLTLERVADV
                     LLLSDGGGGTLLQVPALPPVAIPGLVSPAAPTAVVRELDGHAWLLGLAPVRGQDDRSV
                     GVVVAGRRLEQLERRLAHAGGRPAIVALAGDRLLDATRAGVPAAGWLEAARAGAVQIG
                     GAMWALRPLGGHEALWVAVPTDLAPRRQLWLGLVAALVVMVAAVVAAAWIATRPRRPT
                     GQLAMRRELEALYTAARTMAGGADVRTTAAELLTAACGVARAEAGAVYRLDGDVLTPV
                     AHRGLTPAELSQMGARSLPASLAGEAVRAGRPVVTELRRSGLIEPELVEVARAAGRRL
                     QVALPIGADGAPWGVLILLWGARGEPVGEELRLLEAVAQQIGLAVVRASLEAIAREKS
                     RRLEALTRLGRTLAATLSLDDLLQRVADAAVEVFGASEAGVWLVDDGAGTVSLGAQSP
                     AAAAGAQRLSAGEALAGVVVASRTLLIIADLATDPRVLDIDHARFAGLASFAGVPLTL
                     GEHVLGALTIATLEPHASTDEEVALLQSLASHAAAAIESTRRFAAETRHRDQLADLFE
                     INKRFAAAESAEALLGAIALEAARLLEIDNARFWLVEGGDLVLAGLAGTATETVPRHR
                     IRIGESLPGRVVREDRTIVATLGELHDLLPEHRLADPRAGYLQFLGVPLRTEERIVGV
                     LTFRARRPFTARHRGLAETFAGQAALALERARLYREASRQVERMTALADVERLLTETL
                     DPDVVAQRIADGLPLLVRAHWAGVYRLEAESGDLVGVSVSGAAPWSPRLPAGDGAVGL
                     AVREGRPIVTTDILSDPRITLTPESRAVAEAALHHAVLAMPLVIRDRMIGGISVAHGE
                     EHAFDEEDVRIVQTFANRAAVALENARLFGEAARQAERMAALADVERLLSEALDPNVV
                     AQRIVDSVCSLLGARSAALYGVEAESGALVAVTVSRDVGATFHWTPRLPPGHGLVGLA
                     VAERCPAVAADVLADPRLRYGDEARAAIAAATHRSMVAVPLLVGDRVLGALAVGDVTG
                     RGFAPEELRLARGFADQAALVLENARLFSLETARRSQIEALAAVERELAAELDRERLL
                     HLIIDRASRLFSGFGSIYLLEDGARLVRAACSGSPVGDGQLPVGAGVSGASAAERRGL
                     LVNDYARSPHALAAFKAVGTNRAMAQPLVARDRVLGVVTMSRRGADAPPFSADDLAVL
                     ESFANQTAIVLENARLHDEARRGRDEAHELARVAGVLTESLEVSAVGARVVDSVLTLL
                     SAQSAGLRLLAPDGRLMVVAWRGPESARFEIGHGQPGDAGLAGRAIAEARAVWSADVH
                     ADANLGVPDDIRDRLTRYGHRAVLAVPLFAKGRTIGVLIVADAGPRVFSEAEVALARG
                     FADQAALALDNARLHEEAELRRHEAQFLADLARTINSSLDLGLVLQRITEGAQELCTS
                     DLARIALREPGTERFVYRHWIGARYDGYDGFRIQPGDGASGIVIATGRPFRSDDQESD
                     PRITRVSAEVVRAEGMVTGLTVPIRIDERVDGLLSVHNRTERPFTDRDEAILVQLADH
                     AAIAIRNASLYESVETRAARLRTLADVNRVVSSSLDTVEVLHAIARAASELMDRAFVA
                     FWVADETARTLTFSVVSDPTVAADPPITTLAFGEGVAGQAAAERRTIDVPDVAASDRF
                     RTVAWARAHGIVGFRAVPIMLQGALLGVLALGVGHPLRVDADDEALLDSFVAQAAVAL
                     DHARLYAETRRRLEETGALLTVAEILNSTLDPRQLLERAAVKIAQVCGVSRCTLELWD
                     GDRVTPLVSQFADGHRDEAMWQAFLSVRPYSPRAAPAQARALETRRPVVIEDATASDQ
                     LPREWIETFGPKSYMVVPLIRQDQVMGLLTLDYTERVTPFQDWQVSRATAIAGQLALA
                     LANARLYAEAQERLRETSTLLAVGEALSRPAPLEEGMRALAREVARATGAD"
     gene            complement(6033..7469)
                     /gene="pyk"
                     /locus_tag="VLF19_13010"
     CDS             complement(6033..7469)
                     /gene="pyk"
                     /locus_tag="VLF19_13010"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSE94221.1"
                     /translation="MRRTKIVCTIGPGSASAEQLDRLVAAGMDVARVNFSHGTHAEHA
                     GVIRRLREGEDRWGRPVAILQDLQGPKIRLGTFGAGGGGRVDLETGQAFTLTGKAVVG
                     TAERASVSHPEYLEQVRPGDEIWMDDGMIQLRVEQVGGDEVGCRVVAGGRVSDHKGIS
                     LPHVPLPVSCLTDKDREDLRFGIRQGVDFVAVSFVRSATDIAEVRKFLRDQGADLPIV
                     AKLERQEIVANLPGILTMVDAVMVARGDLGVDVPLEEVPHIQKEVIRQARAAKVPVIV
                     ATQMLESMVTHLRPTRAEVSDVSTAIFDGADAIMLSAETATGRYPVEAVEVMARVATR
                     AEQAVLGDETRRRRREERVGVGFPEAISDAAATAAHVLGARAIVAFTQSGFSARLISQ
                     ERPAVPIIALTPFVEVQRRLALCWGLSSRLIRKVETTDEMIEEVEATLLGDGSVRTND
                     VIVIISGSPMWVTGTTNLLKLHRIGERR"
     gene            complement(7487..10402)
                     /gene="uvrA"
                     /locus_tag="VLF19_13015"
     CDS             complement(7487..10402)
                     /gene="uvrA"
                     /locus_tag="VLF19_13015"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSE94222.1"
                     /translation="MASDRIVIRGAREHNLKNVDLEIPRDQLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLEQMEKPEVDSIEGLSPAISIEQKTTSRNPRSTVGT
                     VTEIYDYLRVLFARVGVPHCPSCGRVISAQTVQQMVDRVLALPSGTRLLVLAPVVRGR
                     KGEYRKLFFDLRRQGYSRVRVNGAVRELTEDIDLDKNRKHTIEVVVDRVVIRENLGSR
                     LADSLETALRLADGIAQVEAVESGGAEPRGARASAGTGSTDMGGGEARAATTDASARL
                     SPPGRSATAVVREPMVFSERLACAQCGISLPEVAPRMFSFNSPYGACEACAGIGTRWE
                     IDPGRLVPNPERSLKDGALAAWAGRESPYFRQTLAVLAKRHRFALDAPWRSLKKAVRD
                     VLLYGEPDGGFEGFVRALERRYRETEAEDARREIEQFMAERDCPDCKGSRLRRESLGI
                     EIGGRSIADVVRFTIKEAAAFFDALTLSEREALIARRVMKEIRERLGFLRDVGLDYLT
                     LDRPAGTLSGGEGQRIRLATQIGSSLVGVLYILDEPSIGLHQRDNRRLLDTLKRLRDL
                     GNTVLVVEHDEETIRSADFVVDLGPGAGELGGHVVAIGTPDEIMANPASLTGRYLSRA
                     LAIPVPPQRRRGAGAWLTVHNPREHNLKGQAVKIPLGTFTCVTGVSGSGKSTLVNDIL
                     YRGLAQMLHRAQDRPGAHDRIEGAQHLDKVIDIDQSPIGRTPRSNPATYTGVFGFIRA
                     LFARTPDARMRGYQPGRFSFNVKGGRCEACQGDGLVKIEMHFLPDVYVTCDVCKGRRY
                     NRETLEVRYKARSIADVLDMTVREALGFFDAVPVIKGKLQTLDDVGLDYIRLGQSATT
                     LSGGEAQRVKLATELSRRATGRTLYILDEPTTGLHFADIQRLLDVLNRLVDAGNTVVI
                     IEHNLDVIKTADFVLDLGPEGGDEGGRVVATGTPEALAAQADRSYTGQFLRPALGRV"
     gene            complement(10444..>10628)
                     /locus_tag="VLF19_13020"
     CDS             complement(10444..>10628)
                     /locus_tag="VLF19_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94223.1"
                     /translation="AAGDPRARAAGAAAGALVLGALAAAAGGLEAAAWPAPALSAWPA
                     LAAAPLAWGVTRVLAR"
BASE COUNT         1462 a         3950 c         3871 g         1345 t
ORIGIN      
        1 gtccgcgccg gtcgcgcggg ccacctcgcg cgccagcgcc cgcatgccct cctcgagggg
       61 cgccggccgc gacagcgcct cgcccacggc gagcagcgtg ctcgtctcgc gcaggcgttc
      121 ctgggcctcg gcgtagaggc gggcgttcgc gagggcgagc gcgagctgcc cggcgatggc
      181 cgtcgcacgg ctcacctgcc agtcctggaa cggcgtcacg cgctcggtgt agtcgagggt
      241 cagcaggccc atgacctggt cctgacggat cagcggcacg accatgtacg acttggggcc
      301 gaacgtctcg atccactcgc gcggcagctg gtcgctcgcg gtcgcatcct cgatcaccac
      361 gggccggcgc gtctcgagcg cgcgggcctg cgcgggtgcc gcgcgcggcg agtacgggcg
      421 cacggagagg aaagcctgcc acatcgcctc gtcgcggtga ccgtcggcga actgcgacac
      481 cagcggggtc acgcggtcgc cgtcccacag ctcgagcgtg cagcggctca ccccgcagac
      541 ctgggcgatc ttcacggcgg cgcgctcgag gagctgccgg ggatcgagcg tggagttgag
      601 gatctcggcg actgtgagga gcgcgccggt ctcctcgagc cggcggcggg tctccgcgta
      661 caggcgggcg tgatcgaggg cgaccgcggc ctgagccacg aagctgtcca gcaacgcctc
      721 gtcgtcggcg tcgacgcgga ggggatggcc gacgccgagg gccagcacac cgagcagcgc
      781 cccctgcagc atgatcggca ccgcccggaa gcccacgatg ccgtgggcgc gcgcccacgc
      841 aaccgtccgg aagcggtcgc tggcggcgac gtcgggcacg tcgatcgtgc gtcgctcggc
      901 ggcggcctgg ccggcgaccc cctcgccgaa ggccagcgtc gtgatcggcg ggtcggcggc
      961 gacggtcggg tccgacacga cgctgaaggt cagcgtacgc gcggtttcgt cggcgaccca
     1021 gaacgccacg aaggcccggt ccatcagctc cgacgccgcc cgggcgatgg cgtgcagcac
     1081 ctcgaccgtg tcgagcgacg aggagacgac gcgattgacg tcggccagcg tgcgcagtcg
     1141 ggccgcgcgc gtctccacgc tctcgtagag cgaggcgttc cggatcgcga tggccgcgtg
     1201 atcggcgagc tggaccagga tggcctcgtc gcggtcggtg aaagggcgtt ctgtccggtt
     1261 gtgcacggac agcaggccgt ccacgcgctc gtcgatccgg atcggcaccg tgagcccggt
     1321 caccatgccc tcggcgcgca cgacctcggc gctgacgcgc gtgatcctcg ggtcgctctc
     1381 ctgatcgtcg ctgcggaacg gacgtccggt ggcgatgacg atgccagacg caccatcgcc
     1441 gggttggatc cggaagccat cgtatccgtc gtaccgcgcg ccgatccagt gccggtagac
     1501 gaaccgctcg gtcccgggct cacgcagggc gatgcgggcg aggtcgctcg tgcacagctc
     1561 ctgcgcaccc tcggtgatgc gctggagcac cagtcccagg tcgagggacg agttgatcgt
     1621 gcgtgccagg tccgcgagga actgggcctc gtgccggcgt agctcggcct cctcgtggag
     1681 gcgcgcgttg tcgagcgcga gcgcggcctg gtcggcgaag ccccgggcca gcgcgacctc
     1741 cgcctccgag aacacgcgcg ggccggcgtc ggcgacgatg aggacgccga tcgtccgccc
     1801 cttggcgaag agcggaacgg cgagcaccgc gcgatgcccg taccgcgtga gtcgatcacg
     1861 aatgtcgtcg gggacgccga ggttggcgtc ggcgtgcacg tccgccgacc acaccgcccg
     1921 cgcctcggcg atggcccgcc cggccagccc ggcgtcccct ggctggccgt ggccaatctc
     1981 gaagcgcgcg ctctcgggcc cccgccaggc gacgaccatc agcctgccgt cgggcgcgag
     2041 cagccgcaag ccggccgact gcgcggacag cagcgtgagc acgctgtcga cgacgcgggc
     2101 gcccacggcg gagacctcga ggctctccgt gaggactccg gccacccggg cgagctcgtg
     2161 cgcctcgtcg cgaccgcgcc gggcctcgtc gtgcagccgg gcgttctcga gcacgatggc
     2221 cgtctggttc gcgaagctct cgagcacggc gagatcgtcg gccgagaacg gcggcgcgtc
     2281 ggcgccgcgg cgggacatgg tgaccacgcc gagcacgcgg tcccgcgcca cgaggggctg
     2341 cgccatcgcg cggttcgtgc ccacggcttt gaaggcggcc agcgcgtggg gcgagcgagc
     2401 gtagtcgttg acgagcaggc cgcggcgctc cgccgcgctc gccccggaca cccccgctcc
     2461 gacaggcagc tggccgtcgc cgacgggcga gcccgagcac gccgcccgga cgagccgggc
     2521 gccgtcctcg agcaggtaga tcgatccgaa gccgctgaag agccggctgg cgcggtcgat
     2581 gatgaggtgg agcaggcgct cgcggtccag ctcggcggcg agctcccgct cgacggcggc
     2641 cagggcctcg atctgcgacc ggcgcgccgt ttcgagcgag aacaggcgcg cgttctccag
     2701 gaccagcgcc gcctggtcgg cgaacccccg ggccaggcgc agctcctccg gggcaaaccc
     2761 gcgcccggtc acgtcgccga cggccagcgc gcccagcacc cggtcgccca cgagcagggg
     2821 caccgcgacc atcgaccggt gtgtcgccgc cgcgatcgcc gcgcgggcct cgtcgccata
     2881 gcgcaggcgg ggatccgcca ggacatccgc ggcgacggcc gggcaccgtt cggcgacggc
     2941 cagcccgacc aggccatggc cgggcggcag ccggggagtc cagtggaacg tcgctcccac
     3001 gtcgcgcgag accgtgacgg cgacgagcgc cccggactcg gcttccacgc cgtagagggc
     3061 ggcggagcgc gcgccgagca gcgaacacac gctgtccacg atgcgctggg ccacgacgtt
     3121 ggggtccagt gcctcggaga gcagacgctc gacgtcggcc agcgccgcca tccgctcggc
     3181 ctggcgcgcg gcctcgccga agagccgggc gttctcgagg gccaccgcgg cccgattggc
     3241 gaacgtctgg acgatccgga cgtcctcctc gtcgaaggcg tgttcctcgc cgtgggccac
     3301 gctgatccct ccgatcatcc ggtcgcggat caccagcggc atcgcgagga cggcgtggtg
     3361 gagagcggcc tccgcgacgg cgcggctctc gggggtgagc gtgatgcgag gatcgctcaa
     3421 gatatccgtc gtcacgatgg ggcgcccctc gcgcacggcc agcccgaccg ctccatcgcc
     3481 ggccgggagc cgggggctcc acggcgcggc gcccgacacc gacaccccga cgaggtcgcc
     3541 gctctcggcc tcgaggcggt agacgcccgc ccagtgggcc cgcacgagga ggggcaggcc
     3601 gtcggcgatc cgctgcgcga cgacgtccgg atccagcgtc tcggtcagca ggcgctccac
     3661 gtccgccagg gccgtcatgc gctccacttg ccggctcgcc tcgcggtaca ggcgggcgcg
     3721 ctcgagcgcc agggccgcct ggcccgcgaa cgtctcggcg agtccgcgat gccgcgccgt
     3781 gaacggccgc cgcgcgcgga acgtcagcac gccgacgatg cgctcctcgg tccgcagcgg
     3841 gacgccgagg aactgcaggt agccggcgcg cgggtcggcc aggcggtgct ccggcagcag
     3901 gtcgtgaagc tcgccgaggg tggcgacgat cgtccggtcc tcgcgcacga cgcggcccgg
     3961 caggctctca ccgatccgga tccggtgccg cggcaccgtc tccgtggccg taccggcgag
     4021 tccggcgagc accaggtcgc cgccctccac gagccagaag cgcgcgttgt cgatctcgag
     4081 cagacgcgca gcctccaggg cgatcgctcc gagcagcgcc tcggcagact cggcggcggc
     4141 gaagcgcttg ttgatctcga acaggtcggc gagctggtcg cggtggcgcg tctcggcggc
     4201 gaagcgccga gtgctctcga tcgcagccgc cgcgtggctc gccagcgact gcagcaatgc
     4261 gacctcctcg tcggtggagg cgtggggctc gagggtggcg atcgtcaagg cgccgagcac
     4321 gtgctctccg agcgtcagcg ggacgccggc gaaggacgcg aggcccgcga agcgcgcgtg
     4381 gtcgatatcg agcacgcggg ggtcggtggc gaggtcagcg atgatcagga gtgtccgcga
     4441 cgcgacgacc acgcccgcca gcgcctctcc ggcagagagc cgctgcgcgc cggcggcggc
     4501 cgcggggctc tgggcgccga gcgacaccgt cccggcgccg tcgtccacga gccagacccc
     4561 ggcctcgctg gccccgaaga cctcgaccgc cgcgtccgcg acgcgctgga gcaggtcgtc
     4621 gagcgagagc gtcgccgcca gcgtgcggcc caggcgcgtc agcgcctcga gccggcggct
     4681 cttctcgcgc gcgatggcct cgagcgaggc ccgcacgacg gcgaggccga tctgctgggc
     4741 cacagcctcc agcagccgga gctcctcgcc taccggctcg ccgcgtgcgc cccacaggag
     4801 gatcagcacg ccccacggcg cgccgtcggc gccgatcgga agcgccacct gcagccggcg
     4861 acccgcggcg cgcgcgacct cgaccagctc gggctcgatc aggccagacc ggcgcaactc
     4921 cgtgaccacg gggcggccgg cccgcaccgc ctcccccgcg aggctcgcgg gcagcgaccg
     4981 cgcacccatc tgcgagagct cggcaggggt caggccgcgg tgggcgaccg gcgtcagcac
     5041 gtcgccgtcg aggcgataca ccgcacccgc ctccgcgcgc gcgacgccgc acgcggcggt
     5101 gagcagctcg gccgcggtcg tccgcacgtc ggcgccaccg gccatcgtgc gcgcggccgt
     5161 atagagcgcc tcgagctcgc ggcgcatggc cagttgtccc gtgggccgcc gggggcgcgt
     5221 cgcgatccac gccgcggcca cgacggccgc gaccatgacg accagcgccg cgaccaggcc
     5281 aagccagagc tgccggcgcg gcgcgagatc ggtgggcacg gcgacccaca gggcttcgtg
     5341 gccgccgagc gggcgcaacg cccacatggc gccgccgatc tgcacggccc ccgcccgggc
     5401 cgcttcgagc cagccggcgg cggggacgcc ggcgcgggtg gcgtcgagca gccggtcgcc
     5461 cgccagcgcc acgatggccg ggcgtcctcc agcgtgcgcg agccgtcgct cgagctgctc
     5521 gagccggcgg ccggcgacga cgacaccgac gctgcggtcg tcctgcccgc gcacgggggc
     5581 gaggccgagc agccacgcgt ggccatcgag ctcgcgcacg acggccgtcg gcgcggccgg
     5641 cgatacgaga cccggaatcg ccaccggcgg gagcgccggc acctgtaaca gcgtgccgcc
     5701 gccgccgtcg gacagcagga gcacgtcggc gacacgctcc agcgtgaggg tgagcatgcg
     5761 cggcgacgtg ctgcgcgtca gcgcggccag gtccccccgc gccgcgccgt ccacgacggc
     5821 cccatcgcgc gcgagcatcg ccgcctcgcg ggcgagggcc tgcgcctcct cgtcgaaggc
     5881 ggcccgcgcg gcggccacca cctcctcgac ccgagcttcc tcgcgcccgg cggcctgccg
     5941 gaggagcgcg ccggcacccg cgccccagag ggcgacgacc gccgcgacga ccagggcaag
     6001 ccggacgagg agagccaggg ccggcacgct cctcagcggc gctcgccgat ccggtggagc
     6061 ttcaagaggt tggtcgttcc cgtcacccac atgggcgagc ccgagatgat gacgatcacg
     6121 tcgttggtcc gcaccgagcc gtcgccgagc agcgtggcct cgacctcctc gatcatctcg
     6181 tcggtggtct cgaccttccg gatcagtcgc gaggagaggc cccagcacag cgccaggcgg
     6241 cgctgcacct cgacgaaggg cgtgagcgcg atgatcggca ccgcggggcg ctcctgggag
     6301 atcagccggg cggagaaccc cgactgggtg aaggccacga tcgcccgcgc accgagcacg
     6361 tgcgccgccg tcgccgctgc gtccgagatg gcctccggga agcccacgcc cacacgctcc
     6421 tcgcgccgcc gccggcgggt ctcgtcgccg agcaccgcct gctcggcgcg cgtggccacg
     6481 cgcgccatca cctccaccgc ctcgaccgga taccggccgg tggcggtctc cgccgagagc
     6541 atgatcgcgt cggcgccgtc gaagatcgcg gtggagacgt cggacacctc ggcgcgcgtc
     6601 gggcggaggt gcgtgaccat cgattccagc atctgcgtgg cgacgatcac cggcaccttg
     6661 gcggcgcggg cctggcggat gacctccttc tggatgtgcg gcacctcctc gagcgggacg
     6721 tcgacaccca ggtcgccacg ggccaccatc accgcgtcca ccatcgtgag gatcccgggc
     6781 aggttcgcga cgatctcctg gcgctcgagc ttggccacga tgggcagatc ggcgccctgg
     6841 tcgcgcagga acttgcggac ctccgcgatg tccgtcgcgg accgcacgaa cgagacggcc
     6901 acgaagtcca cgccctggcg gatgccgaag cggagatcct cgcggtcctt gtcggtgagg
     6961 cacgacacgg gcagcggcac gtgcggcagc gagatgccct tgtgatcgct cacccggcca
     7021 cccgccacca cgcggcagcc cacctcgtcg ccgccgacct gctccacgcg gagctggatc
     7081 atgccgtcgt ccatccagat ctcgtcgccc ggacgcacct gctcgaggta ctccggatgc
     7141 gagacgctgg cgcgctcggc ggtccccacg acggccttgc cggtgagcgt gaacgcctgg
     7201 ccggtctcga ggtccacgcg cccaccgccg ccggcgccga acgtgccgag tcggatcttc
     7261 gggccctgca ggtcttgcag gatcgccacc ggccggcccc accgatcctc gccctcgcgc
     7321 aggcggcgga tgaccccggc gtgctcggcg tgcgtgccgt gggagaagtt cacgcgggcg
     7381 acgtccatgc cggcggcgac gagccggtcc agctgctcgg cgcttgcgct gcccggcccg
     7441 atggtgcaca cgatcttggt tcggcgcatg ggactcctcc cggcgctcag acgcgcccga
     7501 gcgccggcct caggaactgg ccggtgtagg accggtccgc ctgggcggcg agcgcctcgg
     7561 gcgtaccggt ggcgacgacg cgcccgccct cgtcgccgcc ctcgggcccg aggtcgagga
     7621 cgaagtccgc ggtcttgatg acgtcgagat tgtgctcgat gatcaccacg gtgttgccgg
     7681 cgtccacgag ccggttcagc acgtcgagca ggcgctggat gtcggcgaag tgcagacccg
     7741 tcgtcggctc gtcgaggatg tagagggtgc ggccggtcgc ccggcgcgag agctctgtcg
     7801 ccagcttcac ccgctgcgcc tcgcccccgg acagcgtggt cgccgactgg ccgagacgga
     7861 tgtagtcgag gccgacgtcg tcgagcgtct ggagcttccc cttgatgacg ggcaccgcgt
     7921 cgaagaaccc cagtgcctcc cgcaccgtca tgtcgagcac gtcggcgatg gacctggcct
     7981 tgtagcgcac ctcgagcgtc tcgcggttgt agcgccgccc cttgcagacg tcgcacgtca
     8041 cgtagacgtc gggcaggaag tgcatctcga tcttcaccag gccgtcgccc tgacaggcct
     8101 cgcagcgccc gcccttgacg ttgaacgaga agcgcccggg ctggtagcca cgcatgcggg
     8161 cgtcgggcgt gcgggcgaag agcgcccgga tgaagccgaa cacgcccgtg taggtggccg
     8221 ggttcgagcg cggggtgcgg ccgatcgggg actggtcgat gtcgatcacc ttgtcgaggt
     8281 gctgggcacc ctcgatgcgg tcgtgcgcgc ccggccggtc ctgggcgcgg tgcagcatct
     8341 gcgccaggcc gcggtagagg atgtcgttga ccaacgtgga cttgccggat ccgctcaccc
     8401 cggtcacgca cgtgaacgtc cccagcggga tcttcaccgc ctggcccttc aggttgtgct
     8461 cgcgcggatt gtggaccgtg agccacgccc ccgcgcccct gcggcgctgg ggaggcacgg
     8521 gaatcgccag ggcgcgagag agatagcgtc ccgtcagcga cgcgggattg gccatgatct
     8581 cgtcgggcgt gccgatggcg accacgtggc cgccgagctc gccggcgccc ggccccagat
     8641 cgaccacgaa gtcggccgag cggatggtct cctcgtcgtg ctccaccacg agcacggtgt
     8701 tgccgaggtc gcgaaggcgt ttgagcgtgt cgagcagccg ccggttgtcg cgctggtgca
     8761 atccgatgga cggctcgtcg aggatgtaga ggacgcccac gaggctcgac ccgatctgcg
     8821 tggccaggcg gatgcgctgg ccctcgccgc cggacagggt gccggccggg cggtccagcg
     8881 tcaggtagtc gagcccgacg tcgcggagga agccgagccg ctcgcggatc tccttcatca
     8941 cccggcgcgc gatgagcgcc tcgcgctcgg acagcgtgag cgcgtcgaag aacgccgccg
     9001 cctccttgat cgtgaaccgc acgacgtcgg cgatcgaccg gcccccgatc tcgatgccga
     9061 gcgactcgcg gcggagccgc gaccccttgc agtcggggca gtcgcgctcg gccatgaact
     9121 gctcgatctc gcgccgggcg tcctcggcct ccgtctcgcg gtagcgccgc tcgagcgcgc
     9181 gcacgaagcc ctcgaagccg ccgtccggct cgccgtagag gagcacgtcg cgaacggcct
     9241 tcttgagcga cctccacggc gcgtcgagcg cgaaccggtg ccgcttggcc agcacggcca
     9301 gggtctgcct gaagtagggc gactcccgtc ccgcccaagc agccagcgcg ccgtccttga
     9361 gcgagcgctc ggggttcggc accaggcgcc cggggtcgat ctcccagcgg gtgccgatac
     9421 cggcgcaggc ctcgcaggcg ccataggggc tgttgaacga gaacatgcgc ggtgccacct
     9481 cgggcagcga gatgccgcac tgcgcgcacg ccagccgctc cgagaacacc atgggctccc
     9541 ggacaacggc cgtcgcggat cgccccggcg gggagagcct ggccgaggcg tccgtcgtcg
     9601 ccgcccgtgc ctcgccccct cccatgtcgg tcgatcccgt gcccgcggag gccctggccc
     9661 cccgtggctc ggccccaccc gactcgacgg cctccacctg tgcgatgccg tcagccagcc
     9721 gcagcgcggt ctccagcgag tcggccaggc gcgagccgag gttctcgcgt atcaccacgc
     9781 ggtccacgac gacctcgatc gtgtgcttgc ggttcttgtc gaggtcgatg tcctcggtca
     9841 gctcgcgcac ggcgccgttc acgcgcacgc gcgagtaacc ctgccggcgc aggtcgaaga
     9901 agagcttgcg gtactccccc ttgcggcccc gcaccaccgg cgctagcacg agcagccggg
     9961 ttcccgacgg cagcgccagc acccggtcca ccatctgctg caccgtctgg gcggagatca
    10021 cccggccgca gctcgggcag tgcggcacgc ccacgcgcgc gaagagcacg cgcaggtagt
    10081 cgtagatctc cgtgacggtg ccgacggtgg agcgcgggtt ccgggacgtc gtcttctgct
    10141 cgatggagat ggccggcgac aacccctcga tggaatcgac ctcgggcttt tccatctgct
    10201 cgaggaactg gcgggcgtag gccgagagcg actccacgta gcgccgctgg ccctcggcgt
    10261 agatcgtgtc gaacgcgagc gaggacttgc ccgagccgga cagcccggtg atgacgacga
    10321 gctgatcgcg cgggatctcg agatcgacgt tcttgaggtt gtgctcgcgc gccccgcgga
    10381 tgacgatgcg gtcgctcgcc atggtcagcc tattctgtca gcagcgccgc gtggcggcaa
    10441 caatcaccgc gcgagcacgc gcgtcactcc ccacgcgagc ggcgccgccg ccagcgccgg
    10501 ccacgccgag agcgccggcg ccggccatgc ggccgcctcg agtccgccgg cggccgcggc
    10561 cagggcgccc aggaccagcg cgcccgctgc ggcgcccgcc gcgcgcgctc gcgggtcgcc
    10621 ggcggcga
//
LOCUS       DATIAZ010000379         6052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_786351, whole genome shotgun sequence.
ACCESSION   DATIAZ010000379 DATIAZ010000000
VERSION     DATIAZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786351"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VLF19_13025"
     CDS             complement(<1..349)
                     /locus_tag="VLF19_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSE94224.1"
                     /translation="MAKQVLSLTVNDEAVEVLVQPYDTLLDSLREGLGLTGPKEGCGT
                     GDCGACTVHLDGKPVASCLVLAMQARGRSVKTIEGLGRRDALHPLQDAFVRHGVPQCG
                     FCIPGVLMAAAALL"
     gene            complement(461..1327)
                     /locus_tag="VLF19_13030"
     CDS             complement(461..1327)
                     /locus_tag="VLF19_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HSE94225.1"
                     /translation="MRRFELAMPGSVDECLQMLVRRGPDAKMVAGGTDLLPQMKNGLL
                     KPALVVDLSGVAALHAVRRDDGRGLRVGAAVTARTLEQHEHVRSRYPSLAESAALVGS
                     VQIRNLATVGGNVCNAAPSADMAAPLVALEAEAVIAGPRGERRVPVAEFFTGVRRTVL
                     APDELLVEFVVPAPGPRSGGQYVRHTPRRELDIAVVGVASQLTLTDGVCGKARIALAA
                     VAPTPVRATAAERALEGQRVTPELIERAAGLALEAARPISDQRGSADFRRHLVRVLTR
                     RTLTTALARADH"
     gene            complement(1482..2927)
                     /locus_tag="VLF19_13035"
     CDS             complement(1482..2927)
                     /locus_tag="VLF19_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSE94226.1"
                     /translation="MPTGQIFVNGAWRAPASGETYAPVNPATEEPISQVARGDERDVD
                     AAVGAARKAFDQGPWPRLSPHERGRVVWRLGELIQQHLDEMARLESLCTGKTLFDSGK
                     VEIPFAAEVFRYYAGWATKIHGETLALRENAFTFTLRHPVGVVGAIVPWNFPFLLSSW
                     KLAPALAAGNTVVLKPASQTPLTALKFAELAAEAGLPEGVLNVVTGPGGKVGMAMVRD
                     PRVDKIAFTGSTEVGKQIMREGAGTLKRLSLELGGKSPNIVFADADMEAAVRGALTGI
                     FYNKGEVCAAGSRLFLEERIHDEFMAKLTERVKGLKVGDPMDRSTRMGPVVSKQQMDT
                     VLGYIDSGKQEGARLVAGGGRAAVGDGKGYFVEPTIFDGVTNRMKIAREEIFGPVLAV
                     IPFKSVEEGIAEGNATTYGLAAAVWTRDVAKALKAARAIRAGTVWVNAYNLFDAALPF
                     GGFKESGFGREMGSAGLDHYTEVKTVWVDLS"
     gene            complement(2961..4559)
                     /locus_tag="VLF19_13040"
     CDS             complement(2961..4559)
                     /locus_tag="VLF19_13040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HSE94227.1"
                     /translation="MTWSLLLRGGTLIDGTGAPRHPADVAIEGDRIAVVAPGLAGEAA
                     RVIDATGHVVAPGFIDAHSHSDLFYFACPSAESKIRQGVTTEVVGMCSFSQAPLRPEQ
                     RDVVHGWAGGLGANLDLRWETFGQYLDVLRAMRPSVNVAHFVGHGALRIAAMGFEGRP
                     AGADDLRAMERLLGEALDAGAFGFSTGLVYPPSAYAETGELIALAKTMSRRGGLYFSH
                     IRGESSMLLDSIAEAIRIGEESGVGVQISHVKASGRENWPKIDAALRLIEDARTRGVD
                     VLGDVYPYNAGSTKMDNLMPAWALEGGVTKLLERLADRATRRRIVEECLVDGERWRNV
                     SQGGTGFDQIFVASCRRRELEGLHLAHIARQTGRPPAEALMDLLLEERCTVGMVSFSQ
                     SLENVAKVLAHPNLMIGSDSIPLFEGEGDKPGKPHPRTYGTFPRVVGEYARERRLFSL
                     ETAVHKMTGMPAARLGFRDRGLVRTGYAADLTVFDPAIVKDESTYADPHRYPSGISHV
                     IVNGAVVVDDNRLGSARPGRVLIP"
     gene            complement(4647..5081)
                     /locus_tag="VLF19_13045"
     CDS             complement(4647..5081)
                     /locus_tag="VLF19_13045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSE94228.1"
                     /translation="MAQTAVITLESGGEITIEFFPQDAPGTVENFVTLAKKGFYDGLV
                     FHRVVPDFVVQGGDPKGNGTGGPGYTIKAEFNKRKHVRGSLAMARSQHPDSAGSQFYI
                     TYGTQPHLDNNYTVFGQVVSGMEHVDRIKQGDRMRSVAIVEQ"
     gene            complement(5091..5660)
                     /locus_tag="VLF19_13050"
     CDS             complement(5091..5660)
                     /locus_tag="VLF19_13050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSE94229.1"
                     /translation="MIRRLAPIFVIALLGSGIAAAQPKEPPKSTAPPAPKASAAVITM
                     ESGGQVRLEFWPGDAPNHVKNFLDLARKGFYDGQLVHRVEPGFVVQFGDPQTKKLPVN
                     DPRVGGGGPGYTIKAEFNKRPFDRGVLGMARTDDPHSAGSQVYLMLGPAHFLNGQYTA
                     FGRVTSGMDVVDRIKVGDRITSIKVVDDK"
     gene            5750..5932
                     /locus_tag="VLF19_13055"
     CDS             5750..5932
                     /locus_tag="VLF19_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSE94230.1"
                     /translation="MDPVTAVDARLEVLRGRPIDSDPTRVRFCSLASLRRTLGLHFAV
                     EEIVVRGGAARGRVVR"
     gene            5937..>6052
                     /locus_tag="VLF19_13060"
     CDS             5937..>6052
                     /locus_tag="VLF19_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSE94231.1"
                     /translation="MQQRDDVRNIAIIAHVDHGKTTLVDAMLWQSGLFRENQ"
BASE COUNT          876 a         2166 c         2111 g          899 t
ORIGIN      
        1 cgagcaacgc cgccgcggcc atgagcaccc ccgggatgca gaacccgcac tggggcacgc
       61 cgtgacgcac gaacgcgtcc tgcagcgggt ggagggcgtc gcgccgcccg agcccttcga
      121 tcgtcttcac cgaacggcca cgagcctgca tggccagcac gaggcagctc gcgaccggct
      181 tgccgtcgag gtggacggtg caggccccgc aatccccggt gccgcagcct tccttcggcc
      241 ccgtcaggcc aaggccctcg cgcagtgaat ccagcagcgt gtcgtagggc tgcacgagca
      301 cctcgaccgc ttcgtcgttg accgtgaggc tcagtacctg cttggccacg tggcgactcc
      361 ttgcctggtt caggaaactc agcgggcccc agaggcgcgc cacggctgcg ccggggcgtg
      421 ccgaacgctg gggttggggg ccatctcggg gccccccatg tcaatgatcc gcgcgggcga
      481 gcgcggtggt cagcgtgcga cgcgtcagga cacggaccag gtgacgccgg aagtcggccg
      541 agccacgctg gtcgctgatc gggcgcgccg cctcgagggc cagtcccgcc gcgcgctcga
      601 tcagctcggg ggtcacgcgc tggccctcga gcgcccgctc ggccgccgtc gcccgcaccg
      661 gcgtcggcgc caccgcggcg agggcgatcc gtgccttgcc gcacacgccg tccgtcagcg
      721 tgagctgcga ggccacgccg accacggcga tgtccagctc gcggcgcggc gtgtggcgga
      781 cgtactggcc cccgcttcgg gggcccggcg cgggaacgac gaactcgacc agcagctcgt
      841 cgggagccag caccgtgcgc ctcacgccgg tgaagaattc cgcgaccggc acgcgtcgct
      901 cgccgcgagg acccgcgatg accgcctcgg cctccagcgc cacgagcggg gccgccatgt
      961 cggcggaggg cgcggcgttg cacacgttgc cgccgaccgt cgccaggttg cggatctgca
     1021 ccgatccgac cagggcggcg ctctcggcga gcgagggata gcgcgacctc acgtgctcgt
     1081 gctgctcgag cgtccgcgcg gtcaccgccg caccgaccct gagaccgcgc ccgtcgtcac
     1141 ggcgcaccgc gtgcagcgcg gccacaccgg acagatccac cacgagggcg ggcttgagca
     1201 gaccgttctt catctggggc agcaggtcgg tccctcccgc caccatcttc gcgtcgggcc
     1261 cacggcggac gagcatctgg aggcactcgt ccacgctgcc cggcatcgcc agctcgaatc
     1321 gtcgcacgcg ctgttctccg tgaagtaggt cggggcgaag cgccccggtc tcgtcggccg
     1381 cctgggacgt tacatgctcg tccgggccac gtcaacgatc gccccggttt tggctggcgg
     1441 cctctatcta tgccccgacc ggcatgctat cgcaactcgc gtcaggagag gtcgacccag
     1501 acggtcttga cctccgtgta gtggtcgagc cccgccgagc ccatctcgcg cccgaacccg
     1561 gactccttga agccaccgaa gggcagggcg gcgtcgaaca ggttgtaggc gttgacccac
     1621 acggtgccgg cgcggatggc gcgcgcggcc ttcagcgcct tggcgacgtc gcgcgtccac
     1681 accgccgcgg ccaggccgta cgtggtggcg ttgccctcgg cgatgccctc ctcgacggac
     1741 ttgaacggga tcaccgcgag gacgggcccg aagatctcct cgcgggcgat cttcatccgg
     1801 ttggtcacgc cgtcgaagat cgtcggctcg acgaagtacc ccttgccgtc gccgacggca
     1861 gcgcgcccgc ccccggccac caggcgggcg ccttcctgct tgcccgagtc gatgtagccg
     1921 agcaccgtgt ccatctgttg cttgctgacg acgggcccca tgcgcgtgga cctgtccatc
     1981 gggtcgccga ccttgaggcc cttcacccgc tccgtcagct tcgccatgaa ctcgtcgtgg
     2041 atcctctcct cgaggaacag gcgcgagccc gcggcgcaca cctcgccctt gttgtagaag
     2101 atgccggtca gggcgccgcg gacggcggcc tccatgtcgg cgtcggcgaa cacgatgttg
     2161 ggggacttgc cgcccagctc cagcgacagt cgcttcaagg tgcccgcgcc ctcgcgcatg
     2221 atctgcttgc ccacctcggt ggagccggtg aacgcgatct tgtccacgcg cgggtcgcgt
     2281 accatcgcca tccccacctt gccgccaggg ccggtgacca cgttgagcac gccctcgggc
     2341 aacccggcct ccgcggcgag ctcggcgaac ttcagcgccg tgagcggcgt ctgcgaggcc
     2401 ggcttgagga cgacggtgtt cccggccgcc agcgcgggcg ccagcttcca cgaggacaga
     2461 aggaagggga agttccacgg cacgatggcg ccgaccacgc cgacggggtg gcgcagcgtg
     2521 aacgtgaatg cgttctcgcg gagcgcgagc gtctcgccgt ggatcttggt ggcccagccc
     2581 gcgtagtagc ggaacacctc ggcggcgaac gggatctcga ccttgccgga gtcgaagagc
     2641 gtcttgcccg tgcagagact ctcgagccgc gccatctcgt cgaggtgctg ctggatcagc
     2701 tcgcccagcc gccacacgac gcggccgcgc tcgtgggggg acagccgcgg ccacggcccc
     2761 tggtcgaagg ccttccgcgc ggcgccgacg gcggcgtcga catcgcgctc gtcgcccctg
     2821 gcgacctggg agatcggttc ttcggtggcg gggttgacgg gcgcataggt ctcgcccgac
     2881 gcgggcgcgc gccaggcacc gttgacgaag atctgtccgg tcggcatggg ggtgacctcc
     2941 tcctctggaa ccagcgcgcg ctaggggatc agcacgcggc ccggtctggc cgagccgagc
     3001 ctgttgtcgt cgacgacgac ggcgccgttg acgatcacgt gggagatgcc gctcggatag
     3061 cggtgcggat cggcgtacgt ggactcgtcc ttcacgatgg cggggtcgaa cacggtcagg
     3121 tccgccgcgt accccgtgcg cacgagcccg cggtcgcgga aaccgagccg cgccgccggc
     3181 atcccggtca tcttgtggac cgccgtctcc agggagaaga gccgccgctc ccgcgcgtac
     3241 tcgccgacga cgcgcgggaa cgtgccatac gtccgcggat gcggcttgcc gggcttgtcg
     3301 ccctccccct cgaacagcgg aatcgagtcg gagccgatca tgaggttcgg gtgggcgagc
     3361 accttggcga cgttttcgag gctctgcgag aagctgacca tcccgaccgt acaccgctcc
     3421 tccagcagca gatccatcaa cgcctcggcc ggcggccggc ccgtctgccg cgcgatgtgg
     3481 gcgaggtgga gcccctccag ctcgcgccgg cggcaggagg ccacgaagat ctgatcgaag
     3541 ccggtgccgc cctgcgagac gttgcgccag cgctcgccgt ccaccaggca ctcctcgacg
     3601 atgcgccggc gcgtggcgcg gtcggccagg cgctccagca gcttcgtgac accgccctcg
     3661 agcgcccacg cgggcatgag gttgtccatc ttggtgctgc cggcgttgta ggggtagacg
     3721 tcgccgagca cgtcgacgcc gcgcgtgcgg gcgtcctcga tcagccgcag ggcggcgtcg
     3781 atcttcggcc agttctcccg ccccgaggcc ttgacgtgcg agatctgcac gccgaccccg
     3841 ctctcctcac cgatgcggat ggcctcggcg atcgagtcga gcagcatgga agattcgccg
     3901 cggatgtggg aaaagtagag tccgccgcgg cggctcatcg tcttcgccag cgcgatcagc
     3961 tcgccggtct cggcataggc gctgggcgga tagacgagcc cggtcgagaa gccgaaggcg
     4021 cccgcgtcca gcgcctcgcc caggagccgc tccatcgcgc gcaggtcgtc cgcgccggcc
     4081 ggccggccct cgaagcccat ggccgcgatg cgcagggcgc cgtgtccgac gaagtgcgcg
     4141 acgttcacgg acgggcgcat cgcccgcagc acgtcgaggt actgcccgaa ggtctcccag
     4201 cgaaggtcca ggttggcgcc gaggccgccc gcccagccgt gcaccacgtc gcgctgctcg
     4261 ggacgcagcg gcgcctggga gaacgagcac atgcccacga cctcggtggt cacgccctgc
     4321 cggatcttcg actccgccga cgggcacgcg aagtagaaga ggtcggagtg cgagtgcgcg
     4381 tcgatgaagc cgggggcgac gacgtggccg gtcgcgtcga tgacgcgcgc ggcctcgccg
     4441 gcgaggcccg gcgcgaccac ggcgatccgg tcgccctcga tggccacgtc ggccgggtgg
     4501 cgcggcgcgc cggtgccgtc gatcagggtg ccaccgcgga gcagcaacga ccaggtcatc
     4561 tgctctcgga gggggcctcg acggcccccg gcgagacctc ccccgggaca gattgcgccg
     4621 gcaaagccga cgctcggaac gcgcgactac tgctcaacga tggccaccga gcgcatccgg
     4681 tcgccctgct tgatgcggtc gacgtgctcc atgccggaga cgacctgacc gaagaccgtg
     4741 tagttgttgt cgagatgcgg ctgcgtgccg tacgtgatgt agaactggct gcccgcggag
     4801 tcgggatgct ggctgcgcgc catcgccagc gagccgcgca cgtgcttgcg cttgttgaac
     4861 tcggccttga tggtgtagcc gggcccgccg gtgccgttgc ccttcgggtc gccgccctgc
     4921 accacgaagt cggggacgac ccgatggaac acgaggccat cgtagaaccc cttcttcgcc
     4981 agcgtcacga agttctcgac ggtcccgggc gcgtcctggg ggaagaactc gatcgtgatc
     5041 tccccgccgc tctcgagcgt gatgaccgcg gtctgcgcca cgaacgcctc ctacttgtcg
     5101 tcgacgacct tgatggacgt gatgcggtcg ccgaccttga tcctgtccac cacgtccatg
     5161 ccgctcgtca cgcggccgaa cgccgtgtac tggccgttca ggaagtgcgc cggcccgagc
     5221 atgaggtaga cctggctgcc ggccgaatgg ggatcgtccg tgcgcgccat gccgagcaca
     5281 ccccggtcga acggccgctt gttgaactcc gccttgatcg tgtaccccgg cccgccgccg
     5341 cccacgcggg ggtcgttgac cggaagcttc ttcgtctggg ggtcgccgaa ctggacgacg
     5401 aaccccggct cgacccggtg cacgagctgg ccgtcgtaga accccttgcg cgccaggtcc
     5461 aggaagttct tcacgtggtt gggagcgtca ccgggccaga actcgagccg cacctggccg
     5521 cccgactcca tggtgatgac ggccgcgctc gccttgggcg cgggtggcgc cgtggacttc
     5581 ggcggctcct tgggctgggc cgcggcgatg cccgacccga gcagggcgat gacgaagatc
     5641 ggagcgagcc ggcggatcat cgtgaccctc ccgaggcgtg agatgggcgc cattataccc
     5701 acgaccctcg gggtcacatg atttgcccga gggcgtcgcg cgacccgaca tggacccggt
     5761 gacggcggtg gacgcccgac tcgaggtcct gcgcgggcgg ccgatcgaca gcgacccgac
     5821 tcgtgtgagg ttctgctcgc tcgcgtcgct acgccggacg ctcggcctgc acttcgcggt
     5881 ggaggagatc gtggtgcgtg gtggggccgc ccgggggcgc gtggtacgat gaagcgatgc
     5941 agcagcgcga cgacgtccgg aacatcgcga tcatcgccca cgtcgaccac ggcaagacca
     6001 cgctcgtgga cgcgatgctc tggcaatcgg gcctcttccg cgagaaccag ga
//
LOCUS       DATIAZ010000380         5394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilota bacterium isolate
            SMAG_U16277 k141_1709240, whole genome shotgun sequence.
ACCESSION   DATIAZ010000380 DATIAZ010000000
VERSION     DATIAZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935772
            Sequence Read Archive: SRR25158332
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilota bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilota bacterium
            Bacteria; Candidatus Methylomirabilota.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:16:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,618
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,578
            CDSs (with protein)               :: 2,578
            Genes (RNA)                       :: 16
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 14
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 1 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Candidatus Methylomirabilota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709240"
                     /isolate="SMAG_U16277"
                     /isolation_source="soil"
                     /db_xref="taxon:2072417"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..498
                     /locus_tag="VLF19_13065"
     CDS             112..498
                     /locus_tag="VLF19_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSE94232.1"
                     /translation="MRSILVVEDDPETRQALGEYLATFFPEARVLMTDSGETGLELAR
                     RTRPSVVLLDLRLKGMGGFEFGERLRRLPAGAGVPIVALTGDMRPDTLLKAEAAGFVA
                     FLRKPADMDRLETVLRPLFEGTTRAS"
     gene            520..1344
                     /locus_tag="VLF19_13070"
     CDS             520..1344
                     /locus_tag="VLF19_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSE94233.1"
                     /translation="MHDREHVIELRGTVGVVTGGASGIGRALSAGLAREGVRVVVADL
                     DEAGMAETVASIRALGGEAIGVRTDVSDLAQVQVLAERAFGAFGRVHVLCNNAGVATW
                     GGLETATHRDWQWVLGVNLWGVINGLEAFLPRMIAQHEGGHVLNTASMAGLIASQGLG
                     VYNASKYAVVGLSETLAKDLRPYGIGVSVLCPMGVATGIRASERSRPAHLRNEATRGS
                     EPTELIGRTLTADGVAAMAIEAIRDNRLYIITHEEGLEPLRRRFQRLEQAILQRKT"
     gene            1341..2477
                     /locus_tag="VLF19_13075"
     CDS             1341..2477
                     /locus_tag="VLF19_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HSE94234.1"
                     /translation="MTASSPWVRTVADLERLARRLREAGEIALDTEGDSLHHYPERLA
                     LIQIADRAGNAWLVDPLALPDLGALAPVFAAKAPLLVLHAGDNDLAHLKRRHAFTFGA
                     IFDTSLAARFLGGRALGLDVLLREHLGVELPPSRQKDDWSVRPLSPAQELYAVADVEH
                     LLALKDQLVERLERAGRRAWVEEECAALASEVVPERAPDPDAWTRLKGARDLSPRGLA
                     ILRAVHETRERLALATARPPFKILGDATLVAMALAPPPDAAALAALPGCSPRVVARWG
                     DAVLEAVRRALALPEAELPVLARPTRPPGLSGAVRRRIEALRAWRSEAAPRLDLDPGV
                     LLPNRLIGPIAQAAPRTRDELARVEGIRRWRVDALGEGILAAIP"
     gene            2495..2941
                     /locus_tag="VLF19_13080"
     CDS             2495..2941
                     /locus_tag="VLF19_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSE94235.1"
                     /translation="MNDWRPRLVSDDDGLRAILREAKRIAVLGAKRGEGEPSFYVPRY
                     LESRGARIVPVNPTLAGERLFGVAVVPRLADLAEPVDVVDVFRRPEYLPAHAGEILAL
                     PWRPRAVWFQLGIRHDGAAETLARAGIRVVQDRCMMPEHRRLLGSA"
     gene            2960..4648
                     /gene="ettA"
                     /locus_tag="VLF19_13085"
     CDS             2960..4648
                     /gene="ettA"
                     /locus_tag="VLF19_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HSE94236.1"
                     /translation="MGAVAGEYAFVMKDLRKVVPPRREILRGIWLSFYHGAKIGVLGA
                     NGAGKSSLLRIMAGADQDFLGEAFPAAGLRIGYLSQEPTLDASKDVRGNVEEGVAATR
                     ALLTRFEEVSAKLGEPLDGDEMEKLLEEQARLQDGIDASSAWDLDHTVEMAMDALRCP
                     PGDADVGQISGGERRRVALCRLLLSRPDMLLLDEPTNHLDAESVAWLERYLKEYPGTI
                     VAVTHDRYFLDNVAGWILELDRGHGIPWEGNYSSWLAQKHERLAREEKTDLARQRTLA
                     RELEWVRLAPRARHAKSRARLQAYEAMAAESYEQRAERLEIAIPPGPRLGDVVIEAEG
                     VGKAYGDRVLIEDLSFKLPRGGIVGVIGPNGAGKTTLFRMIGGQEKPDRGTLRVGETV
                     KLAYVDQSRDALDPTKTVWEEISGGAETLSLGGRTVPSRAYVASFNFKGSDQQKRVGD
                     LSGGERNRLHLATMLTTGGNVLLLDEPTNDLDVDTLRALEDALLEFAGCVVVISHDRW
                     FLDRIATHMLAFEDEGRVVWFEGNYQDYEADRKRRLGAEAERPHRLKFKRVPRA"
     gene            complement(4752..>5394)
                     /locus_tag="VLF19_13090"
     CDS             complement(4752..>5394)
                     /locus_tag="VLF19_13090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSE94237.1"
                     /translation="TRSALSGRIRVIDGVRERRLIVAGDTLSVLPLDGDWARLRPEYW
                     WQALTIAPLPLRPAVLLVGLGGGTQLHLLTRLAPPRTVTVIERDPAILRVARDWFGLG
                     TIGGVEYLCGGAEAVVPWLGRIGRRFDYIMEDAAYADTPERARPLALSVASLVAPGGV
                     LVLNRHWRSDAAGVAALLRPRFARVTQRRVRRGGENVLICCAGPASVTAAAWA"
BASE COUNT          746 a         1939 c         1972 g          737 t
ORIGIN      
        1 cgatctcggt gatcccgcgc gcgtgctcgc ctcactgcga cgcaccggcc ggcgtccggc
       61 ctggctcggc gactcggagc tcgcctcgac ggcgtagcgt acactggcgc catgcgatcg
      121 atcctcgtgg tcgaggacga ccccgagacg cggcaggcgc tgggggagta cctcgccacc
      181 ttcttccctg aggcgcgcgt gctcatgacc gattccggcg agacgggcct cgagctcgcc
      241 cgccgcaccc gcccgagtgt cgtgctgctc gacctccgtc tcaagggcat gggtggcttc
      301 gagttcggcg agcggctgcg tcgtctgccc gccggcgccg gcgtgcccat cgtcgcgttg
      361 accggggaca tgaggccgga taccttactg aaggccgagg cggccggctt cgtcgccttc
      421 ctccggaagc ccgcggacat ggatcgcctg gagacggtgc tccgcccgct cttcgagggc
      481 accacgcgcg cgagctgacc gggctcgacc caaggcggat tgcacgaccg ggagcatgtc
      541 atcgagcttc gaggcacggt aggagtcgtc acgggcggcg ccagcggcat cggccgggcg
      601 ctatcggcgg ggcttgcgcg cgagggcgtg agggtcgtcg tggccgatct cgacgaggcc
      661 ggcatggccg agaccgtcgc ctcgatccgt gccctcggcg gcgaggcgat cggcgtcagg
      721 accgacgtga gcgacctcgc ccaggtgcag gtcctggccg agcgggcatt cggcgccttc
      781 ggccgcgtgc acgtcctgtg caacaacgcc ggcgtggcga cctggggtgg actcgagacg
      841 gcgacccatc gggactggca gtgggtgctg ggcgtgaacc tgtggggcgt catcaacggc
      901 ctcgaggcgt tcctgccgcg catgatcgcg cagcatgagg gcgggcacgt cctcaacacg
      961 gcgtcgatgg cgggcctgat cgcctcccag gggctcgggg tctacaacgc gtcgaagtac
     1021 gccgtggtcg gcctgtcgga gacgctcgcc aaggacctca ggccttacgg gatcggtgtc
     1081 tccgtgctgt gcccgatggg ggtggccaca ggcatccgcg cgagcgagcg ctcgcggccc
     1141 gcgcacctca ggaacgaggc cacccggggg tcggagccca cggagctgat cgggcgcacg
     1201 ctcacggccg acggcgtcgc ggccatggcc atcgaggcga tccgcgacaa ccgcctctat
     1261 atcatcaccc acgaggaggg gctcgagccg ctgcggcgac ggttccagcg gctcgagcag
     1321 gcgatcctcc agcgaaagac ctgaccgcgt cgtccccctg ggtccggacc gtcgccgatc
     1381 tcgagcgact cgcgcgccgc ctgcgcgagg cgggcgagat cgccctcgac accgagggtg
     1441 acagcctcca ccactacccc gagcgcctgg ccctcatcca gatcgccgac cgtgccggaa
     1501 acgcctggct cgtcgacccc ctggcgttgc cggatctcgg cgccctggcg ccggtgttcg
     1561 cggcgaaggc accgctgctg gtcctgcacg ccggcgacaa tgacctcgcg cacctcaagc
     1621 ggcgtcacgc cttcaccttc ggcgcaatct tcgacacctc gctggccgcg cgcttcctgg
     1681 gcggccgcgc gctcggcctc gacgtgctcc tgcgcgagca tctcggcgtg gagctcccgc
     1741 cgtcgcgaca gaaggacgac tggtccgtgc ggccgctctc cccggcgcag gagctctacg
     1801 cggtggccga cgtcgagcac ctgctggcgc tcaaggacca actcgtggag agactggagc
     1861 gcgccggtcg ccgcgcctgg gtcgaggagg agtgcgcggc gctggcctcc gaggtcgtgc
     1921 ccgagcgcgc gccggatccc gacgcgtgga cgcggctcaa gggcgcccgg gacctgtcgc
     1981 cgcgggggtt ggcgatcctg cgcgcggtgc acgagacgcg cgagcggctg gcgctcgcca
     2041 ccgcccgccc gccgttcaag atcctcggtg acgcgacgct ggtggcgatg gcgctggcgc
     2101 cgcctccgga cgccgccgcg ctcgctgctc tgcctgggtg ctcgccgcgg gtcgtcgcgc
     2161 gctggggcga cgccgtgctc gaggccgtgc gccgcgcgct ggcgctgccc gaggccgagc
     2221 tgcccgtgct cgcccgcccg acccgcccgc ccggcctgtc gggcgccgtg cggcggcgta
     2281 tcgaggcgct ccgtgcctgg cgcagcgagg cggccccgcg cttggatctc gatcccggcg
     2341 tcctcctgcc caaccggctc atcggcccga tcgcccaggc cgcgccgcgc acccgtgacg
     2401 agctggcgcg ggtcgagggc atccgccggt ggcgcgtcga cgcgctgggc gaggggatcc
     2461 tcgccgcgat cccgtgacct gtcataatga gcgcgtgaac gactggcggc cgaggctcgt
     2521 gagcgacgac gatggcctgc gcgcgatcct gcgcgaggcc aagcgcatcg ccgtgctcgg
     2581 cgccaagcgt ggcgagggcg agccgtcgtt ctacgtaccg cgttacctcg agtcacgcgg
     2641 tgcccggatc gtcccggtga atcccacgct ggcgggggag cggctgttcg gcgtggcggt
     2701 cgtgccgagg ctcgcggacc tcgccgagcc ggtggacgtg gtcgacgtct tccgccggcc
     2761 cgaatacctg ccggcgcacg cgggggagat cctcgccctg ccgtggcgcc cgcgggcggt
     2821 gtggtttcag ctcggcatcc gtcacgacgg cgcggccgag acactggcgc gcgcgggcat
     2881 ccgcgtggtg caggaccgct gcatgatgcc cgagcaccgc cgcctgctcg ggtcggcgtg
     2941 atcggcgcgc tccgaaagga tgggcgcggt ggctggtgaa tacgcgttcg tgatgaagga
     3001 cctcaggaag gtcgtgccgc ccaggcggga gatcctgcgc gggatctggc tgtcgttcta
     3061 ccacggggcg aagatcggcg tgctcggcgc caacggcgcc ggcaagtcct cgctcctgcg
     3121 catcatggcc ggcgccgacc aggatttcct gggcgaggcg tttccggccg cgggactgcg
     3181 gatcggctat ctctcgcagg agccgacgct cgacgcctcg aaggacgtgc gtggcaatgt
     3241 ggaggagggt gtcgcggcga cgcgagccct gctcacgcgc ttcgaggagg tctcggcgaa
     3301 gctcggcgag ccgctcgacg gcgacgagat ggagaagctg ctcgaggagc aggcgaggct
     3361 tcaagacggg atcgacgcct ccagcgcctg ggatctcgac cacacggtcg agatggcgat
     3421 ggacgcgctg cgctgcccgc ccggcgacgc cgacgtgggg cagatctcgg gtggcgagcg
     3481 gcggcgcgtg gcgctctgcc ggctgctgct gtcgcgcccc gacatgctgc tgctcgacga
     3541 gccgaccaac cacctcgacg ccgaatcggt cgcgtggctg gagcgctatc tcaaggagta
     3601 ccccgggacc atcgtcgcgg tgacgcacga ccggtatttt ctcgacaacg tcgccgggtg
     3661 gatcctggag ctcgaccggg gccacgggat cccctgggag ggcaactact cgtcgtggct
     3721 cgcccagaag cacgagcggc tcgcgcgaga ggagaagacc gacctcgccc gccagcggac
     3781 gctcgcgcgg gagctcgagt gggtgcgcct ggcgccccgg gcccgccacg ccaagagtag
     3841 ggcgcgcctg caggcctacg aggcgatggc cgccgagtcg tacgagcagc gcgccgagag
     3901 gctcgagatc gcgatcccgc cggggccgcg cctcggcgac gtcgtgatcg aggccgaggg
     3961 agtcggcaag gcctacggcg accgcgtgct catcgaggac ctctcgttca agctgccgcg
     4021 cggcggcatc gtcggcgtca tcggccccaa cggggccggc aagaccacgc tcttccgcat
     4081 gatcggcggt caggagaagc ccgaccgtgg cacgctcagg gtcggcgaga ccgtgaagct
     4141 cgcctacgtc gaccagagcc gggacgctct cgacccgacg aagaccgtgt gggaggagat
     4201 ctcgggcggc gccgagacgc tgtcgctcgg cggccggacc gtgccgtcgc gggcctacgt
     4261 cgcctccttc aatttcaagg gctccgacca gcagaagcgc gtcggcgacc tctccggcgg
     4321 cgagcgcaac cgcctgcatc tcgccacgat gctgacgacc ggcggcaacg tcctgctgct
     4381 cgacgagccg acgaacgacc tcgacgtcga caccctccgg gcgctggagg atgcgctgct
     4441 cgagttcgcg ggctgcgtcg tcgtcatcag ccacgaccgg tggttcctcg atcgcatcgc
     4501 cacgcacatg ctcgccttcg aggacgaggg acgcgtggtg tggttcgagg gcaactatca
     4561 ggactacgag gccgaccgca agcgccgcct cggcgccgag gccgagcggc cccaccgcct
     4621 caagttcaag cgcgtgccgc gcgcttagcc tcccgagtcc gaagtgcgtc gtcgcgcggc
     4681 ggcgggcggg gtgggcatga ggtgagagtg ctcgcacccg cctcatccga ctcgccacaa
     4741 ggccgcgtgg gtcaggccca cgccgccgcg gtgacgctgg ccggcccggc gcaacagatc
     4801 aggacgttct cgccacctcg gcggacgcga cgttgcgtca cccgcgcgaa ccgcggccgc
     4861 agcagagccg ccacgccggc cgcgtcgctc cgccaatggc ggttcagcac gagcacgccc
     4921 cccggcgcaa cgagcgaggc cacggagagt gcgaggggcc gggcgcgctc gggggtgtcg
     4981 gcatacgccg cgtcctccat gatgtagtcg aagcgccgcc cgatccgccc gagccacggg
     5041 accaccgcct ccgcgccgcc gcacaggtat tcgacgccgc cgatggtgcc gagtccgaac
     5101 cagtcgcgcg ccacgcgcag gatggcggga tcacgctcga tgacggtgac ggtgcgcggc
     5161 ggcgccagcc gcgtgagcag atgaagctgg gtcccgcccc ccagccccac gagcagcacc
     5221 gccggacgga gcggcaaggg ggcgatggtc agcgcctgcc accagtactc ggggcggagg
     5281 cgcgcccagt cgccgtcgag cggcagcacc gacagcgtgt ccccggccac gatcagccgc
     5341 cgctccctca caccgtcgat gacgcggatc cggccggaca gcgccgaccg cgtc
//
